LOCUS       DAJTRW010000001       136796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRW010000001 DAJTRW010000000
VERSION     DAJTRW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            172..1605
                     /gene="cscA"
                     /locus_tag="OO326_000001"
     CDS             172..1605
                     /gene="cscA"
                     /locus_tag="OO326_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5964558.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1613..2671)
                     /locus_tag="OO326_000002"
     CDS             complement(1613..2671)
                     /locus_tag="OO326_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5964559.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2839..3057
                     /locus_tag="OO326_000003"
                     /pseudo
     CDS             2839..3057
                     /locus_tag="OO326_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3075..4403
                     /gene="dsdA"
                     /locus_tag="OO326_000004"
     CDS             3075..4403
                     /gene="dsdA"
                     /locus_tag="OO326_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5964560.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4511..6049)
                     /gene="emrY"
                     /locus_tag="OO326_000005"
     CDS             complement(4511..6049)
                     /gene="emrY"
                     /locus_tag="OO326_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5964561.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6049..7212)
                     /gene="emrK"
                     /locus_tag="OO326_000006"
     CDS             complement(6049..7212)
                     /gene="emrK"
                     /locus_tag="OO326_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5964562.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7628..8242
                     /gene="evgA"
                     /locus_tag="OO326_000007"
     CDS             7628..8242
                     /gene="evgA"
                     /locus_tag="OO326_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5964563.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8247..11840
                     /gene="evgS"
                     /locus_tag="OO326_000008"
     CDS             8247..11840
                     /gene="evgS"
                     /locus_tag="OO326_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5964564.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11896..13041)
                     /gene="yfdE"
                     /locus_tag="OO326_000009"
     CDS             complement(11896..13041)
                     /gene="yfdE"
                     /locus_tag="OO326_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5964565.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13115..14059)
                     /gene="yfdV"
                     /locus_tag="OO326_000010"
     CDS             complement(13115..14059)
                     /gene="yfdV"
                     /locus_tag="OO326_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5964566.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14129..15823)
                     /gene="oxc"
                     /locus_tag="OO326_000011"
     CDS             complement(14129..15823)
                     /gene="oxc"
                     /locus_tag="OO326_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5964567.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15877..17127)
                     /gene="frc"
                     /locus_tag="OO326_000012"
     CDS             complement(15877..17127)
                     /gene="frc"
                     /locus_tag="OO326_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5964568.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17640..18263)
                     /locus_tag="OO326_000013"
     CDS             complement(17640..18263)
                     /locus_tag="OO326_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5964569.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18560..18835
                     /locus_tag="OO326_000014"
     CDS             18560..18835
                     /locus_tag="OO326_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964570.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18912..19154)
                     /locus_tag="OO326_000015"
     CDS             complement(18912..19154)
                     /locus_tag="OO326_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5964571.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19507..20427
                     /gene="lpxP"
                     /locus_tag="OO326_000016"
     CDS             19507..20427
                     /gene="lpxP"
                     /locus_tag="OO326_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5964572.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20782..20853
                     /gene="ypdK"
                     /locus_tag="OO326_000017"
     CDS             20782..20853
                     /gene="ypdK"
                     /locus_tag="OO326_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5964573.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20918..22156)
                     /gene="alaC"
                     /locus_tag="OO326_000018"
     CDS             complement(20918..22156)
                     /gene="alaC"
                     /locus_tag="OO326_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5964574.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22533..24019
                     /locus_tag="OO326_000019"
                     /pseudo
     CDS             22533..24019
                     /locus_tag="OO326_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24034..24768
                     /gene="ypdB"
                     /locus_tag="OO326_000020"
     CDS             24034..24768
                     /gene="ypdB"
                     /locus_tag="OO326_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5964575.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24781..25638
                     /locus_tag="OO326_000021"
                     /pseudo
     CDS             24781..25638
                     /locus_tag="OO326_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25641..28136)
                     /gene="ptsP"
                     /locus_tag="OO326_000022"
     CDS             complement(25641..28136)
                     /gene="ptsP"
                     /locus_tag="OO326_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5964576.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28161..29198)
                     /gene="ypdE"
                     /locus_tag="OO326_000023"
     CDS             complement(28161..29198)
                     /gene="ypdE"
                     /locus_tag="OO326_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5964577.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29198..30281)
                     /gene="ypdF"
                     /locus_tag="OO326_000024"
                     /pseudo
     CDS             complement(29198..30281)
                     /gene="ypdF"
                     /locus_tag="OO326_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30296..31543)
                     /locus_tag="OO326_000025"
     CDS             complement(30296..31543)
                     /locus_tag="OO326_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5964578.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31565..31891)
                     /locus_tag="OO326_000026"
     CDS             complement(31565..31891)
                     /locus_tag="OO326_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5964579.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32110..33075)
                     /gene="glk"
                     /locus_tag="OO326_000027"
     CDS             complement(32110..33075)
                     /gene="glk"
                     /locus_tag="OO326_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5964580.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33279..34535
                     /locus_tag="OO326_000028"
     CDS             33279..34535
                     /locus_tag="OO326_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5964581.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34650..34976
                     /locus_tag="OO326_000029"
     CDS             34650..34976
                     /locus_tag="OO326_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5964582.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35117..36355)
                     /locus_tag="OO326_000030"
     CDS             complement(35117..36355)
                     /locus_tag="OO326_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5964583.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36691..37893
                     /gene="nupC"
                     /locus_tag="OO326_000031"
     CDS             36691..37893
                     /gene="nupC"
                     /locus_tag="OO326_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5964584.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37943..40055)
                     /locus_tag="OO326_000032"
                     /pseudo
     CDS             complement(37943..40055)
                     /locus_tag="OO326_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40263..40338)
                     /locus_tag="OO326_000033"
     tRNA            complement(40263..40338)
                     /locus_tag="OO326_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40303..40305),aa:Ala,seq:ggc)
     gene            complement(40378..40453)
                     /locus_tag="OO326_000034"
     tRNA            complement(40378..40453)
                     /locus_tag="OO326_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40418..40420),aa:Ala,seq:ggc)
     gene            40674..41033
                     /locus_tag="OO326_000035"
     CDS             40674..41033
                     /locus_tag="OO326_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5964585.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41035..41427
                     /locus_tag="OO326_000036"
     CDS             41035..41427
                     /locus_tag="OO326_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5964586.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41479..42894)
                     /gene="gltX"
                     /locus_tag="OO326_000037"
     CDS             complement(41479..42894)
                     /gene="gltX"
                     /locus_tag="OO326_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5964587.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITYWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43153..43228
                     /locus_tag="OO326_000038"
     tRNA            43153..43228
                     /locus_tag="OO326_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43186..43188,aa:Val,seq:tac)
     gene            43273..43348
                     /locus_tag="OO326_000039"
     tRNA            43273..43348
                     /locus_tag="OO326_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43306..43308,aa:Val,seq:tac)
     gene            43353..43428
                     /locus_tag="OO326_000040"
     tRNA            43353..43428
                     /locus_tag="OO326_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43386..43388,aa:Lys,seq:ttt)
     gene            43459..43584
                     /locus_tag="OO326_000041"
     ncRNA           43459..43584
                     /ncRNA_class="other"
                     /locus_tag="OO326_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43704..44045
                     /locus_tag="OO326_000042"
     CDS             43704..44045
                     /locus_tag="OO326_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5964588.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44036..44962)
                     /locus_tag="OO326_000043"
     CDS             complement(44036..44962)
                     /locus_tag="OO326_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5964589.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45052..46050
                     /locus_tag="OO326_000044"
     CDS             45052..46050
                     /locus_tag="OO326_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5964590.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46047..46265)
                     /locus_tag="OO326_000045"
     CDS             complement(46047..46265)
                     /locus_tag="OO326_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5964591.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46267..48282)
                     /gene="ligA"
                     /locus_tag="OO326_000046"
     CDS             complement(46267..48282)
                     /gene="ligA"
                     /locus_tag="OO326_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5964592.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48353..49339)
                     /gene="zipA"
                     /locus_tag="OO326_000047"
     CDS             complement(48353..49339)
                     /gene="zipA"
                     /locus_tag="OO326_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5964593.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49569..50330
                     /gene="cysZ"
                     /locus_tag="OO326_000048"
     CDS             49569..50330
                     /gene="cysZ"
                     /locus_tag="OO326_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5964594.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50515..51486
                     /gene="cysK"
                     /locus_tag="OO326_000049"
     CDS             50515..51486
                     /gene="cysK"
                     /locus_tag="OO326_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5964595.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51870..52127
                     /gene="ptsH"
                     /locus_tag="OO326_000050"
     CDS             51870..52127
                     /gene="ptsH"
                     /locus_tag="OO326_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5964596.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52172..53899
                     /gene="ptsI"
                     /locus_tag="OO326_000051"
     CDS             52172..53899
                     /gene="ptsI"
                     /locus_tag="OO326_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5964597.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53940..54449
                     /gene="crr"
                     /locus_tag="OO326_000052"
     CDS             53940..54449
                     /gene="crr"
                     /locus_tag="OO326_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5964598.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54491..55342)
                     /gene="pdxK"
                     /locus_tag="OO326_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54491..55342)
                     /gene="pdxK"
                     /locus_tag="OO326_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5964599.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55447..55821
                     /locus_tag="OO326_000054"
     CDS             55447..55821
                     /locus_tag="OO326_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5964600.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55854..56577
                     /locus_tag="OO326_000055"
                     /pseudo
     CDS             55854..56577
                     /locus_tag="OO326_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56781..57692)
                     /gene="cysM"
                     /locus_tag="OO326_000056"
     CDS             complement(56781..57692)
                     /gene="cysM"
                     /locus_tag="OO326_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5964601.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57826..58923)
                     /gene="cysA"
                     /locus_tag="OO326_000057"
     CDS             complement(57826..58923)
                     /gene="cysA"
                     /locus_tag="OO326_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5964602.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58913..59788)
                     /gene="cysW"
                     /locus_tag="OO326_000058"
     CDS             complement(58913..59788)
                     /gene="cysW"
                     /locus_tag="OO326_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5964603.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59788..60621)
                     /gene="cysT"
                     /locus_tag="OO326_000059"
     CDS             complement(59788..60621)
                     /gene="cysT"
                     /locus_tag="OO326_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5964604.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60621..61637)
                     /gene="cysP"
                     /locus_tag="OO326_000060"
     CDS             complement(60621..61637)
                     /gene="cysP"
                     /locus_tag="OO326_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5964605.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61941..62732)
                     /gene="ucpA"
                     /locus_tag="OO326_000061"
     CDS             complement(61941..62732)
                     /gene="ucpA"
                     /locus_tag="OO326_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5964606.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62919..63719)
                     /gene="murR"
                     /locus_tag="OO326_000062"
     CDS             complement(62919..63719)
                     /gene="murR"
                     /locus_tag="OO326_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5964607.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63883..64779
                     /gene="murQ"
                     /locus_tag="OO326_000063"
     CDS             63883..64779
                     /gene="murQ"
                     /locus_tag="OO326_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5964608.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64783..66207
                     /gene="murP"
                     /locus_tag="OO326_000064"
     CDS             64783..66207
                     /gene="murP"
                     /locus_tag="OO326_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5964609.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66387..67286)
                     /gene="yfeX"
                     /locus_tag="OO326_000065"
     CDS             complement(66387..67286)
                     /gene="yfeX"
                     /locus_tag="OO326_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5964610.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67382..67957)
                     /locus_tag="OO326_000066"
     CDS             complement(67382..67957)
                     /locus_tag="OO326_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5964611.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68018..68467)
                     /locus_tag="OO326_000067"
     CDS             complement(68018..68467)
                     /locus_tag="OO326_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5964612.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68454..68879)
                     /locus_tag="OO326_000068"
     CDS             complement(68454..68879)
                     /locus_tag="OO326_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5964613.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69093..69962
                     /gene="amiA"
                     /locus_tag="OO326_000069"
     CDS             69093..69962
                     /gene="amiA"
                     /locus_tag="OO326_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5964614.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69966..70865
                     /gene="hemF"
                     /locus_tag="OO326_000070"
     CDS             69966..70865
                     /gene="hemF"
                     /locus_tag="OO326_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5964615.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70871..71923)
                     /gene="eutR"
                     /locus_tag="OO326_000071"
                     /pseudo
     CDS             complement(70871..71923)
                     /gene="eutR"
                     /locus_tag="OO326_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71969..72469)
                     /gene="eutK"
                     /locus_tag="OO326_000072"
     CDS             complement(71969..72469)
                     /gene="eutK"
                     /locus_tag="OO326_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5964616.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72482..73141)
                     /gene="eutL"
                     /locus_tag="OO326_000073"
     CDS             complement(72482..73141)
                     /gene="eutL"
                     /locus_tag="OO326_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5964617.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74323..75114
                     /locus_tag="OO326_000074"
     CDS             74323..75114
                     /locus_tag="OO326_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964618.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75834..76229
                     /locus_tag="OO326_000075"
     CDS             75834..76229
                     /locus_tag="OO326_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5964619.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76247..76510
                     /locus_tag="OO326_000076"
     CDS             76247..76510
                     /locus_tag="OO326_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964620.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76533..77075
                     /locus_tag="OO326_000077"
     CDS             76533..77075
                     /locus_tag="OO326_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964621.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77214..77501
                     /locus_tag="OO326_000078"
     CDS             77214..77501
                     /locus_tag="OO326_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964622.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77540..77746
                     /locus_tag="OO326_000079"
     CDS             77540..77746
                     /locus_tag="OO326_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964623.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78058..78543
                     /locus_tag="OO326_000080"
     CDS             78058..78543
                     /locus_tag="OO326_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5964624.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78524..78802
                     /locus_tag="OO326_000081"
     CDS             78524..78802
                     /locus_tag="OO326_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964625.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78795..80255
                     /locus_tag="OO326_000082"
     CDS             78795..80255
                     /locus_tag="OO326_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5964626.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80289..81098
                     /gene="murI"
                     /locus_tag="OO326_000083"
     CDS             80289..81098
                     /gene="murI"
                     /locus_tag="OO326_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5964627.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81217..81911)
                     /locus_tag="OO326_000084"
     CDS             complement(join(81217..81665,81665..81911))
                     /locus_tag="OO326_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5964628.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82430..83803)
                     /gene="eutA"
                     /locus_tag="OO326_000085"
     CDS             complement(82430..83803)
                     /gene="eutA"
                     /locus_tag="OO326_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5964629.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83778..84257)
                     /gene="eutP"
                     /locus_tag="OO326_000086"
     CDS             complement(83778..84257)
                     /gene="eutP"
                     /locus_tag="OO326_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5964630.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84270..84605)
                     /gene="eutS"
                     /locus_tag="OO326_000087"
     CDS             complement(84270..84605)
                     /gene="eutS"
                     /locus_tag="OO326_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5964631.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84898..87177)
                     /gene="maeB"
                     /locus_tag="OO326_000088"
     CDS             complement(84898..87177)
                     /gene="maeB"
                     /locus_tag="OO326_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5964632.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87466..88416
                     /gene="tal"
                     /locus_tag="OO326_000089"
     CDS             87466..88416
                     /gene="tal"
                     /locus_tag="OO326_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5964633.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88436..90439
                     /gene="tkt"
                     /locus_tag="OO326_000090"
     CDS             88436..90439
                     /gene="tkt"
                     /locus_tag="OO326_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5964634.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90542..91585)
                     /locus_tag="OO326_000091"
     CDS             complement(90542..91585)
                     /locus_tag="OO326_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5964635.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91711..92286)
                     /gene="nudK"
                     /locus_tag="OO326_000092"
     CDS             complement(91711..92286)
                     /gene="nudK"
                     /locus_tag="OO326_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5964636.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92354..94333)
                     /gene="aegA"
                     /locus_tag="OO326_000093"
     CDS             complement(92354..94333)
                     /gene="aegA"
                     /locus_tag="OO326_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5964637.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94539..96256
                     /gene="narQ"
                     /locus_tag="OO326_000094"
                     /pseudo
     CDS             94539..96256
                     /gene="narQ"
                     /locus_tag="OO326_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96458..99571
                     /gene="acrD"
                     /locus_tag="OO326_000095"
     CDS             96458..99571
                     /gene="acrD"
                     /locus_tag="OO326_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5964638.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99670..99729)
                     /gene="ypfM"
                     /locus_tag="OO326_000096"
     CDS             complement(99670..99729)
                     /gene="ypfM"
                     /locus_tag="OO326_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5964639.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100110..100466
                     /locus_tag="OO326_000097"
     CDS             100110..100466
                     /locus_tag="OO326_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5964640.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100470..101597
                     /gene="dapE"
                     /locus_tag="OO326_000098"
     CDS             100470..101597
                     /gene="dapE"
                     /locus_tag="OO326_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5964641.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101625..101825
                     /locus_tag="OO326_000099"
     CDS             101625..101825
                     /locus_tag="OO326_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5964642.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101935..102633)
                     /gene="ypfH"
                     /locus_tag="OO326_000100"
     CDS             complement(101935..102633)
                     /gene="ypfH"
                     /locus_tag="OO326_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5964643.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102707..104722)
                     /gene="tmcA"
                     /locus_tag="OO326_000101"
     CDS             complement(102707..104722)
                     /gene="tmcA"
                     /locus_tag="OO326_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5964644.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104737..105600)
                     /locus_tag="OO326_000102"
     CDS             complement(104737..105600)
                     /locus_tag="OO326_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5964645.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105768..106481)
                     /gene="purC"
                     /locus_tag="OO326_000103"
     CDS             complement(105768..106481)
                     /gene="purC"
                     /locus_tag="OO326_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5964646.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIDLNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106694..107728)
                     /gene="bamC"
                     /locus_tag="OO326_000104"
     CDS             complement(106694..107728)
                     /gene="bamC"
                     /locus_tag="OO326_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5964647.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107745..108623)
                     /gene="dapA"
                     /locus_tag="OO326_000105"
     CDS             complement(107745..108623)
                     /gene="dapA"
                     /locus_tag="OO326_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5964648.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108769..109341
                     /locus_tag="OO326_000106"
     CDS             108769..109341
                     /locus_tag="OO326_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5964649.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109341..109811
                     /gene="bcp"
                     /locus_tag="OO326_000107"
     CDS             109341..109811
                     /gene="bcp"
                     /locus_tag="OO326_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5964650.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110064..110681
                     /gene="hyfA"
                     /locus_tag="OO326_000108"
     CDS             110064..110681
                     /gene="hyfA"
                     /locus_tag="OO326_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5964651.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110681..112698
                     /gene="hyfB"
                     /locus_tag="OO326_000109"
                     /pseudo
     CDS             110681..112698
                     /gene="hyfB"
                     /locus_tag="OO326_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112709..113656
                     /gene="hyfC"
                     /locus_tag="OO326_000110"
     CDS             112709..113656
                     /gene="hyfC"
                     /locus_tag="OO326_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5964652.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113673..115112
                     /gene="hyfD"
                     /locus_tag="OO326_000111"
     CDS             113673..115112
                     /gene="hyfD"
                     /locus_tag="OO326_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5964653.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115124..115774
                     /gene="hyfE"
                     /locus_tag="OO326_000112"
     CDS             115124..115774
                     /gene="hyfE"
                     /locus_tag="OO326_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5964654.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115779..117371
                     /gene="hyfF"
                     /locus_tag="OO326_000113"
     CDS             115779..117371
                     /gene="hyfF"
                     /locus_tag="OO326_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5964655.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            117361..119075
                     /gene="hyfG"
                     /locus_tag="OO326_000114"
                     /pseudo
     CDS             117361..119075
                     /gene="hyfG"
                     /locus_tag="OO326_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119085..119630
                     /gene="hyfH"
                     /locus_tag="OO326_000115"
     CDS             119085..119630
                     /gene="hyfH"
                     /locus_tag="OO326_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5964656.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119627..120385
                     /gene="hyfI"
                     /locus_tag="OO326_000116"
     CDS             119627..120385
                     /gene="hyfI"
                     /locus_tag="OO326_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5964657.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120378..120791
                     /gene="hyfJ"
                     /locus_tag="OO326_000117"
     CDS             120378..120791
                     /gene="hyfJ"
                     /locus_tag="OO326_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5964658.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120821..122650
                     /gene="hyfR"
                     /locus_tag="OO326_000118"
     CDS             120821..122650
                     /gene="hyfR"
                     /locus_tag="OO326_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5964659.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122651..122833
                     /locus_tag="OO326_000119"
     CDS             122651..122833
                     /locus_tag="OO326_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5964660.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122855..123727
                     /locus_tag="OO326_000120"
                     /pseudo
     CDS             122855..123727
                     /locus_tag="OO326_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123821..124882)
                     /locus_tag="OO326_000121"
     CDS             complement(123821..124882)
                     /locus_tag="OO326_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5964661.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125095..126558
                     /gene="bepA"
                     /locus_tag="OO326_000122"
     CDS             125095..126558
                     /gene="bepA"
                     /locus_tag="OO326_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5964662.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126579..126938
                     /gene="arsC"
                     /locus_tag="OO326_000123"
     CDS             126579..126938
                     /gene="arsC"
                     /locus_tag="OO326_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5964663.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127071..127817)
                     /gene="hda"
                     /locus_tag="OO326_000124"
     CDS             complement(127071..127817)
                     /gene="hda"
                     /locus_tag="OO326_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5964664.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127867..129156)
                     /gene="uraA"
                     /locus_tag="OO326_000125"
     CDS             complement(127867..129156)
                     /gene="uraA"
                     /locus_tag="OO326_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5964665.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129242..129868)
                     /gene="upp"
                     /locus_tag="OO326_000126"
     CDS             complement(129242..129868)
                     /gene="upp"
                     /locus_tag="OO326_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5964666.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130192..131229
                     /gene="purM"
                     /locus_tag="OO326_000127"
     CDS             130192..131229
                     /gene="purM"
                     /locus_tag="OO326_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5964667.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131229..131867
                     /gene="purN"
                     /locus_tag="OO326_000128"
     CDS             131229..131867
                     /gene="purN"
                     /locus_tag="OO326_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5964668.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            132039..134105
                     /gene="ppk1"
                     /locus_tag="OO326_000129"
     CDS             132039..134105
                     /gene="ppk1"
                     /locus_tag="OO326_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5964669.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134110..135651
                     /gene="ppx"
                     /locus_tag="OO326_000130"
     CDS             134110..135651
                     /gene="ppx"
                     /locus_tag="OO326_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5964670.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135690..>136796)
                     /gene="pdeF"
                     /locus_tag="OO326_000131"
     CDS             complement(135690..>136796)
                     /gene="pdeF"
                     /locus_tag="OO326_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5964671.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34151 a        35192 c        33617 g        33836 t
ORIGIN      
        1 ctttctcgca aaatcgtcat ggatttacta aaacatgcat attcgatcac aaaacgccat
       61 agttaacgtt aacatttgcg atattcatcg catttatgaa agtaagggac tatattttta
      121 taaaagttaa cgttaataat tcaccaaatt tgcttaacca ggatgattaa aatgacgcaa
      181 tctcgattgc atgcggcgca aaacgcacta gcaaaacttc atgagcaccg gggtaacact
      241 ttctatcccc attttcacct cgcgcctcct gccggatgga tgaacgatcc aaacggcctg
      301 atctggttta acgatcgtta tcacgcgttt tatcaacatc acccgatgag cgaacactgg
      361 ggaccaatgc actggggaca tgccaccagt gacgatatga tccactggca gcatgagcct
      421 attgcgctag cgctaggaga cgataatgac aaagacgggt gtttttcagg tagtgctgtc
      481 gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt
      541 aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc
      601 gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa
      661 gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg
      721 gggcagatcc tgctttatcg cggcagttca ttgcgtgaat gggccttcga tcgcgtactg
      781 gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc
      841 gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga
      901 aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa
      961 tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta
     1021 gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc
     1081 tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat
     1141 ggcaaacttc tacaacgccc ggttcacgaa gctgagtcgt tacgccagca gcatcaatct
     1201 gtctctcccc gcagaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag
     1261 attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc
     1321 actggaatgc ggctgtatat tgataaccaa tctgagcgac ttattttgtg gcggtattac
     1381 ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc
     1441 ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg
     1501 atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac
     1561 ggagtggctg tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg
     1621 aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa
     1681 taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg
     1741 gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattaca gcgacatcct
     1801 gcgggatacg tacccccttc gccaaaagaa cctgataagc cacaaaggct gcgcgatcgt
     1861 taccacatat cagaacatca aaatctggtt tgcccgattt gaagtgagca ttgagtaaac
     1921 ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca
     1981 gatctcgtcc agcatcacgc caagcctgct caaatccctg ccgacgatac cctgttgcca
     2041 acgcactttc cggtagccag aagcataatg gttgacgata gcccgccgcg agcaaatgct
     2101 gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat
     2161 ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat
     2221 gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg
     2281 ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact
     2341 cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt
     2401 cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg
     2461 aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag
     2521 gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca
     2581 ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt
     2641 agcagaaaca gacgtcactg gcgagatcca acgccctatc acctgacaca gcaatacaat
     2701 aaaaaataac aataattccc ggacaattgt ccccaattcc gcctctgttc tcgccaacga
     2761 gtaaccatat aggcaagtaa gtaaaacatg ccaactggca tcgccaacct ggagaagaaa
     2821 atataaatat ggatcattgt gctgccactg tatcccgaca tcagcccgga aattattgcg
     2881 attgctatcg gttcaggtgc aattggctgc acgatcgtta cggactcgct tttctggctg
     2941 gtgaagcaat attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca
     3001 tttatcgctt cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg
     3061 caaagagacg tactatggaa aacgctaaaa tgaactcgct catcgcccag tatccgttgg
     3121 taaaggatct ggttgctctt aaagaaacca cctggtttaa tcctagcacg acctcattgg
     3181 ctgaaggttt accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct
     3241 tatcccgttt tgcaccctat ctggcaaaag catttgctga aacagctgcc actgggggga
     3301 ttattgaatc agaactggtt gccattccgg ctatgcaaaa acggctggaa aaggaatatc
     3361 aacaaccgat cagcgggcaa ctcctactga aaaaagatag ccatttgccc atttccggct
     3421 ctataaaagc acgcggcggg atttatgaag tcctggcaca tgcagaaaaa ctggctctgg
     3481 aagcggggtt gctgacgctt gatgatgact acagcaaact gctttctccg gagtttaaac
     3541 agttctttag ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg
     3601 gcattatgag cgcccgcatt ggctttaagg tgacagttca tatgtctgct gatgcccggg
     3661 catggaaaaa agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt
     3721 atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta
     3781 ttgatgacga aaattcccgc acgttgttcc ttgggtattc cgttgctggc cagcgtctta
     3841 aagcgcaatt tgcccagcaa ggccgtatcg tcgatgctga taaccctctg tttgtctatc
     3901 tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg
     3961 gcgatcatgt tcactgcttt tttgccgaac caacgcactc cccttgtatg ttgttaggcg
     4021 tccatacagg attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg
     4081 cagcggatgg ccttgcagtt ggtcgcgcat catgctttgt cgggcgggca atggagcgtc
     4141 tgctggatgg cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg
     4201 cgcaggaaga aggtattcgt cttgaacctt cagcactggc gggtatggcc ggacctcagc
     4261 gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt cagcgcagaa caattgcgta
     4321 atgccactca tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatgaatc
     4381 aatatctggc aaaaggccgt taataacgtt tcaacgcaac atcgcaatcc tttccctggg
     4441 tgagcgatgc tgccgatggc gcagacttaa gatccccggt cttacccgct ataaccccct
     4501 ccttttatca tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa
     4561 ccgtaagtaa gataaaagca atagccgcca tacggaaaat ctcatttgca gaaatagaaa
     4621 gtgactgctg ggttatttca ttattaattt cattaagaac tcctgaaagc gaaccatagt
     4681 atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg
     4741 tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac
     4801 caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat
     4861 tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga
     4921 atccctggaa aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca
     4981 cagaacgcca gtaatagcaa accgcataca tcaaaaaact gaatgtcact aacacccgca
     5041 tgtctatttt attgccataa cgtcctatca aaggtgaaat caatagtggc atgatgccga
     5101 tgggcgcata agcaagcccg gcccatatcg cattataccc catcgttttc tggagtaact
     5161 gcggcataag gacgatcgct ccagagtaaa ataaatacgc gcaggtgata ctcacaatac
     5221 caatggtgaa attacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg
     5281 attcccaaat gactaaagag atcagagaaa taactgatac tactgttaat attattattg
     5341 tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cataatttgc aagccaccaa
     5401 caccgagcac taacagggtc agtcttggta gattcatttt taccggtgaa gtttcagttt
     5461 ctcttccttt aagtaaggtt aagcataatg tcaggacgat aatccccata gggacattga
     5521 ttaaaaatat ccaaccccag ctaaagttat cgcaaatata gccgcccaat atcggcccac
     5581 atatcggagc gataatcacg gtcattgacc ataatgccag agcaaatgtt cttttttctg
     5641 gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc
     5701 cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg
     5761 aagacagcga aaaaaaagtg actgaaagta aaaataacct taattcgcct attctttgtg
     5821 ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgag gtgataaccc
     5881 aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag
     5941 aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata gtaacgcacc
     6001 ataacgtccc accggttaat ggtgccggag ttgatttagt gattgccatt aaagttgtcc
     6061 attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc
     6121 cttactggta taagcaggca tggaggtcac ggttgaagcc agatcaggca tctcagcaat
     6181 gtcttcgtcc ttcgtatcaa tagttgcagt catcgataaa ccaatgcgca aggggtgttc
     6241 catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca
     6301 gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat
     6361 ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac
     6421 tgattgacca atccgtacat ccgtgagttg tgtttcttta aagttggcat taacccacat
     6481 ttgacgtgcc ggtacgacag ccattaacga ttgtccggaa ctcactgttt cgccgacctg
     6541 aacacttcgc tgggcaatat agccagtaac cggactcctt atatccgtac gtttaagcac
     6601 caaccaggct tctttggttg catccgccgc ttcaactact tgtggctgac ggtttaatgg
     6661 tgtgttcatt actaaagctt tattcgcttt ataagcctgg atagcggcat tcagtgccgc
     6721 tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg
     6781 cttcgctaaa ggcacacgac ggttataatc ttctaaagat tgttgatact gaatacgtgc
     6841 tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac
     6901 aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag ttttatccag
     6961 tgaaactaaa atgtcaccct gtcgaacgta gttcgtatct ttatgattaa ctacagtgac
     7021 actacctgag acttgtgcag aaattggatc tgcattcccc gtgacatagg cgtcatctgt
     7081 actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa
     7141 taaaactatc accaataaag aaaagtattt ttttctgtta gactgttttt tatttgaatt
     7201 aatctgttcc actattatct ctcatttcgc atagatgaaa tttatgaatt gaatgaaaag
     7261 agtattcatt cttcatatca aacacagtgc atgtgttgca cattatccca ggaacgttgg
     7321 aggcctaatt taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa
     7381 tacaattctt acgcctgtag gattagtgag aagacttata gtgctaactt gaaactataa
     7441 atcatcggta taatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg
     7501 gtatacttgt cgaattatct taaaggaaac tcagattttc ttatttttat tgagaaaatg
     7561 agatgacgcc ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga
     7621 ataatctatg aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa
     7681 tttattgatc aaaaacgata ttgaaatctt agcggagtta actgaaggcg gaagcgccgt
     7741 tcagcgggtg gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt
     7801 taacggtatc caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat
     7861 cgtctccgct aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg
     7921 tttcgtgagt aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa
     7981 tggctactgc tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca
     8041 gcaaaaactc gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg
     8101 caaggataat aatgacattg cggaaaaaat gttcatcagc aacaaaactg tcagcacgta
     8161 taaaagtcgc ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc
     8221 gcaacgtaac aaaatcggct aatcacatga agtttttacc ctattttttt cttctctgtt
     8281 gtggtctttg gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca
     8341 gtagtaacaa ccgtgtcaca cttgatccag tacgtctgag caacaaggaa ttacgttggt
     8401 tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc ccaaacggct accttgttgc
     8461 ataccgattc gcagcaacag gttcgtggta ttaatgctga ttatttaaat cttttaaaaa
     8521 gagcgttaaa tatcaaatta acactccggg aatacgcaga tcatcaaaaa gcaatggacg
     8581 cgcttgcaga aggtgaagtc gatatagtgt tatcacattt agttacttcg ccgcctctta
     8641 ataatgacat tgctgcaacc aaaccattga taattacctt tccggcgctg gtaaccaccc
     8701 ttcacgactc aatgcgaccg cttacctcac caaaaccagt aaatattgct cgggtagcaa
     8761 attacccccc agacgaggta attcatcaat catttccaaa agcaacaatt atctctttta
     8821 caaatttata tcaggcatta gcatccgtct cagctgggca caatgattac tttattggta
     8881 gtaacatcat taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga
     8941 aatattataa ctcgccgcgt caatataatt ttttcttgac cagaaaagaa tctgtcattc
     9001 ttaatgaagt actcaataga tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat
     9061 cacaaaattg gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg
     9121 aacatgaaaa acagtggatt aagcagcatc ccaatttaaa ggtgctggaa aatccttact
     9181 cgccccccta ttctatgacg gatgaaaatg gctcggttcg gggcgttatg ggggacattc
     9241 ttaatattat taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata
     9301 tccatgctgg aacacaactt agccccggag gatgggatat aatacctggc gctatttata
     9361 gtgaagatcg agaaaataat gttttatttg ctgaagcctt cataacaacg ccttacgttt
     9421 ttgtcatgca aaaagcgcct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca
     9481 ttccatatta ttatgagctg cattcgcaat taaaagagat gtatccggag gttgaatgga
     9541 tacaggtcga taatgccagc gctgcatttc acaagattaa ggaaggtgaa cttgatgctc
     9601 tggtcgcgac acagctaaat tcgcgttaca tgatcgatca ttactatcct aatgaacttt
     9661 atcattttct tattcctggc gttccgaatg catcgctttc gttcgctttt cctcgcggag
     9721 aaccggaact taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc
     9781 tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctat
     9841 atagcgagca attttatatt gttacgacat tatccgtttt attagttggc agtagccttt
     9901 tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag
     9961 aaaaccaaat atcattccga aaagcactct cggattcctt accgaatcca acttatgttg
    10021 taaactggca aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg
    10081 attactacaa aaatgcaatg ttaccattag aaaacagtga ttcacccttt aaagatgttt
    10141 tttctaatgc gcatgaagtc acagcagaaa cgaaagaaaa tcgaacaata tacacacagg
    10201 tatttgaaat tgataatggc atcgagaaaa gatgcattaa tcactggcat acattatgca
    10261 atcttcctgc aagtgacaat gcagtatata tttgtggttg gcaagatatt actgaaacgc
    10321 gtgatctaat taatgcactc gaggtagaaa aaaataaagc gataaaggct accgtagcaa
    10381 aaagtcagtt tctggcaacg atgagtcacg aaataagaac accaataagc tctattatgg
    10441 gcttcctgga acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt
    10501 cacttgccta cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg
    10561 acaaaattga atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag
    10621 tccagaacac ttgtcactct ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt
    10681 gcagcagtac atttcctgat cattacctgg ttaagatcga ccctcaggcg tttaagcagg
    10741 tcttatcaaa tttactgagt aatgctctca aatttaccac cgagggggca gtaaaaatta
    10801 cgacctccct gggtcacatt gatgacaacc acgctgttat caaaatgacg attatggatt
    10861 ctggaagtgg attatcgcag gaagaacaac aacaactgtt taagcgctac agccaaacaa
    10921 gtgcaggtcg tcagcaaaca ggttctggtt taggcttaat gatctgcaaa gaattaatta
    10981 aaaatatgca gggcgattta tcattagaaa gtcatccagg cataggaaca acatttacga
    11041 tcacaatccc ggtagaaatt agccagcaag tggcgactgt cgaggcaaaa gcagaacaac
    11101 ccatcacact acctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc
    11161 tattactcaa acgccagcta aatctattag gatatgatgt tgatgaagcc actgatggtg
    11221 tgcaagcgct acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata
    11281 tgccgaatat ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac
    11341 ccatctgggg gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg
    11401 gcatgaactt atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc
    11461 agttacacca ggttgcgcat attgcacctc agtatcgcca ccttgatatc gaagccctga
    11521 aaaataatac ggcgaatgat ctacaactga tgcaggagat tctcatgact ttccagcatg
    11581 aaacgcataa agatctaccc gctgcgtttc atgcactaga agctggcgat aacagaactt
    11641 tccatcagtg tattcatcgc atccacggtg cggctaacat cctgaatttg caaaagttga
    11701 ttaatattag ccatcagtta gaaataacac ctgcttcaga tgacagtaag cctgaaattc
    11761 ttcagctgct gaactctgtt aaagaacaca ttgcagagct ggaccaggag attgctgttt
    11821 tctgtcagaa aaatgactaa attgcggctc ccacaatgtt ctaatgtggg agctatttac
    11881 cagcacatct tacatttatg atgagaactc ctggcgaatt tgttccccat gctggtcgag
    11941 cgttgccgct cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc
    12001 cggcatcatt attcctccgg cttcaatcaa catatttctc gcctgagttt gtggcaaatt
    12061 aatggcctca gccacactta ataacggcgc gacgggcaca ccaacttcat gtattttggc
    12121 taaccaaact tcagctgcct gcgtttttaa cgttcgctca atatattgtt taagaatagc
    12181 ctggttttgt acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag
    12241 ctccagtgcc tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg
    12301 cttatcctga gtatcgaaaa catcaaaagg tgccatgtag ggatggcgat ttcccaggcg
    12361 ttgtggtgac ttccctgtcg caatatatgc catcagacca tgctccagaa aactcagcgt
    12421 ggcatcaaac atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag
    12481 tgcactcact attccgctga ataaatagac accgccgcat agatccgcaa gagaggtacc
    12541 aacacgcact ggcggagcat cagggtaccc tgtttccatc attatcccac tcattgcctg
    12601 aatgatggta tcgtaggcag gagcatcttt tagcggaccg gtatgtccga aacctgacga
    12661 tgaagcatat atgaggcgcg ggttgatttc ttgtaacctt tcccatgaaa accccagttt
    12721 ttccattgta cctaggcgaa aattctcagc taatacatca gcttgtttga gcatatttat
    12781 aaatatactt ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt
    12841 aataaaactg taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc
    12901 accatgacct ggtggctcaa ctttaattac ccttgcgccc atattataaa gaagttgagt
    12961 tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc
    13021 tttgctttca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac
    13081 ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa
    13141 tccacaaggg agccgtgacg acaaagccca gtacgctcac agccaatgac gctgtaccag
    13201 tacgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaatg
    13261 cgcctgccag taccatcatc tgcaaatgtt cgctgttcaa atgacatgcc ataccaacga
    13321 gaagcagcgc cagtggcatc agaatcaact tcaggaaggt gttataagca atttcggcac
    13381 tgaactcgaa tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt
    13441 tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca
    13501 gcaccaggat cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta
    13561 atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca
    13621 gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctacc actaaacctg
    13681 ttgatacgga atcaccataa ataggatcga gaactgcaaa ccccaggaat ccaatggtag
    13741 gtgaacccgc aattaatgca catacagctg cttctgcatg ggtacgttta aaaaatttgt
    13801 agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca
    13861 gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagagccg
    13921 caggaagagc gtagtttaat accagtttat taaatgcgcg ggcttgatct tctgaaaaag
    13981 tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc
    14041 caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca tcgcataatg
    14101 agttggcaag aggagacgat gtcagtattt aattaccagc gacttgtttt gggttaagtt
    14161 tggtaatatg gccactttca gttcctgctg cagggtcgat aaccacatta ataatggtcg
    14221 gtttgcgcga ctgaataccg gtggttaaag catgacgaag ttcatctgtc gtggtgacgt
    14281 tatagccaac gccacgaaac gcatccatta atttgtcata ccttgcatgg tgcaacagat
    14341 ccgttggtga tggtgcacca gcgccactga gatcaacacc gtctcctctg tagatgccgc
    14401 cattattaaa aataacgatc gtcaccggca ggttatatcg acaaatcgtt tcaatttcca
    14461 tcccactgaa accaaaagca ctatcacctt caatggcgac aaccggagaa ccagaagtca
    14521 cgctagcacc gatggcatag cccataccga tgcccatgac accccaggta ccacaatcca
    14581 gacgacgacg tggtttatac atatcaataa tatttcgtgc attatccagg gtatttgcac
    14641 cttcattaac taaataaata tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg
    14701 cattaaagta atttaatggt tgagtatctg tacttaattt ttcatgcatt ttttgtgcat
    14761 tttgctgctt gtggatattt aaaatatcgc gccataccag tggagtcgta aatgtgtttt
    14821 gtttcagttc tgccagcata ccttgcatac tggatgcaat atcaccaacg actggcacag
    14881 caatggggcg gttgctgtca atttcctgcg gttcaatatc cagttgaata aactgtgtat
    14941 ctgccgccca tcctttttta ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca
    15001 tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg
    15061 tatcttcaag gatccctttc gccatagaca ttggcaggaa tggaatctga gcactttcaa
    15121 taaattcacg aagctgttca tcagcttgtg aatacgccgc gcctttgcca aggataatta
    15181 atggccgttc agcttttgct aaaagcgaaa ttgcgctagt gactgacttc gggcatggca
    15241 ataatgctgg cgacggattt tcaactttaa caatcgtggt taacgcttcg tctttttcca
    15301 tcgtcgcggc caggacattt gctggcaaat caagataaac tccgccaggg cgacccgata
    15361 cagagacccg gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa
    15421 atgctgcttt ggcatacggt tttgccgcat tcatttggtc cagctcttca taatcacctt
    15481 gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat catgataatc ggaaaaccat
    15541 ttaccgttgc gttggccaat gcggtcaaac cattgaggaa tcctggcgca gaaactgtca
    15601 ggcagatccc cggtttttgg gtaagaaaac cgcttgccgc agcggcatag cctgccgact
    15661 gctcatgacg aaaaccaata taacgaatgc cttccgcctg ggcatggcgt gccatatccg
    15721 tcacaggaat acctacaaca ccataaatag tgtcaatatt attctgtttt aatgcttcaa
    15781 cgatgatatg cataccatct gtcatttgaa gttgatctga catgattaat aacctcgaaa
    15841 tttttataaa ttgggagcca gaagcccccg ttaatatcag atggcgtggt tttgcttcat
    15901 tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat acagcagcgg tatgttcacc
    15961 taatagcggc gcagctttaa tatccggcgt aaaggcagag aatttcattg gacaaccaac
    16021 ggtcagatat tttccacgca acggttgttc cacttcaaca acactgccac tttggcgcaa
    16081 agagggatca agtgaaattt ctttcatact taaaaccggt gcacaaggaa tatcaaactg
    16141 agtcaaatag gccaccgctt catgtttatc aatagtgaca gtgtattttt cgatttcagc
    16201 aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta ctgtatgccg gatcggtaat
    16261 ccattctggt ttgccgatgg ctttacaggt gttttcccag ttttgctcct gaatagtgaa
    16321 ataaatatag gcgttaggat cggtttccca gcctttacat ttcaggatcc agccaggctg
    16381 accgccacca cctgcattac caccgcgggg aactgcatca ccaaatgtac cattcggata
    16441 ctgcgggtat tcttccagat aacccaattt atcgagacgc tgctggtcac gtaatttcac
    16501 gcggcaaagg ttcaatacgg catcctgcat tgacatggtg actcgttgcc cacgccccgt
    16561 tttttcgcga tgcagcaaag cagcaagtaa accgatcagc aaatgcattc cggtgttgct
    16621 gtcacccaac gctgcagcgc ttaccagcgg cggaccatcc caaaaacccg tagtggatgc
    16681 cgcgccaccc gctgcctgag caacgttttc ataggctttt acattcacat aaggcgaaca
    16741 ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga ttgatttctt gaatatgctc
    16801 ccaggtgaag cccatgtgat caatggcccc tggatgaaag ttctcgacta agatatcagc
    16861 ttcgcggatc agcttttcca ttacctcttt gccttccgct gttttggtat ttaactcaat
    16921 agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg
    16981 cagttggtga cgcgttacgt caccaacgcc gggacgttca attttaataa cgtcagcgcc
    17041 aaaccaggcc agcatttgag tacaagatgg gccagattgc acaccggtga aatcgagaac
    17101 tttaattcct tgaagtggag ttgacatagc aatacctatt agttaattaa atatgtgtta
    17161 atttagcgtt tatttaaggc ataacgccct ctataaataa tctgcaaaat atcttcgtag
    17221 cggcactcga tatttttaaa ttcctgtatc aagtactgaa aattatttta tcgccacttc
    17281 caattccttt ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattaatt
    17341 cgtggtaatc agagagagtc gcgacatttg tgatgttatt cttacaactg taagaagcgc
    17401 cggtgattta caagtaagag tatgattacg ctttttaaag cgtaccataa agcataacat
    17461 aataaatgat ttgtgatgac acgggattat tgcggtttac tttgaggaaa ataaaatgcg
    17521 tgtttataga accgcgagcg ccaaataatt ttatgaccac aacctgtcgt attgtggtca
    17581 taaaaaattg tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcat
    17641 tagttcacaa acagtgcttc gctatcgacg atgattccat cttccgcacc tttaagcgcc
    17701 aggtttgcct cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta
    17761 cgagtctgtt taagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga
    17821 agctcttcca ttgcgccctt tttatcccct ttcgccattt tttcattggc aatttttatc
    17881 gcggcttctt tttcgggtgt tgctacataa ctctcagaaa ttccgacaga tgcattaatg
    17941 acaatatatt ggtcatcatt gagattggtc tttttacctg gcttagcgaa ctttgcccat
    18001 tcggtactgt catctgacag caaagcggaa gcttcattgg tcagttcttt tgccttttcc
    18061 ggatcgccat gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct
    18121 tgttcagaga tacgctctgc tgctgccgtt ttctgggttt gctgagtttg ctgttgtgcc
    18181 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
    18241 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
    18301 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
    18361 atcttacaat tgtagttatt atcgcaaagc ggcatttagt cttacagttg taggaatatg
    18421 cctcctgtcg cagagaacta ttcttatata ccacaaagag atgtcattta ttatattgat
    18481 gtaaatattg attaattata tgtaaataca gccataacat cgtatggttc tcagtgaacc
    18541 attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat ttttattggt
    18601 ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa gtgagaggag
    18661 ctataaagag actgaaaaaa catcagcgat gacgaccaca cactctacaa aacttcagcc
    18721 atcacaggcg attttgttta agatgagaga agatgtgcca ccattaaacc tcacagaaga
    18781 aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct
    18841 gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgagcc
    18901 atcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa gccacaagcc
    18961 ggggcaagaa acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg
    19021 aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc
    19081 acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc gagaaacatc
    19141 cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt
    19201 taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg aaaacagcac
    19261 gttaaatgca acgctattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta
    19321 gctgaaagca gtcagccagt gataaagtag agccaccgca ttcatgctcc tcgtggttat
    19381 gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa
    19441 ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag
    19501 gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc
    19561 acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc
    19621 tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc
    19681 gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg cgagaagatg
    19741 attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat ggcatggttc
    19801 tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa ccttaaacgc
    19861 gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg
    19921 ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc acataataat
    19981 cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc
    20041 agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct
    20101 cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat
    20161 gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc
    20221 gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg
    20281 gaaggctacc cgacagatga aaatcaagcc gctgcttata tgaacaagat tatcgagaaa
    20341 gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg
    20401 gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg
    20461 gccataataa cctcatgatt tttatagctt ttttcttaag ttttttcctt cgctgaatat
    20521 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20581 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc
    20641 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20701 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20761 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20821 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20881 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    20941 gtttgctgct gacgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21001 cctgacgaat acggtcgtgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21061 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21121 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21181 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacgac
    21241 ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21301 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21361 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21421 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21481 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21541 gcacatcgta gcatttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21601 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21661 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21721 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21781 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21841 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21901 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    21961 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22021 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22081 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22141 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22201 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22261 agaacatttt tatcaatgga tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22321 tacgctaaca agaaaatcac caccaatttt attaacatta taatttcaat aaatttacca
    22381 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt taatcccctt
    22441 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22501 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22561 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22621 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22681 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22741 tggtgaacgt acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag
    22801 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22861 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22921 tgaaaatccc caaagcacag cgctggcgct ggatatcgcc aacaaaacgc taccgctgtt
    22981 tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa attatccgcg atgacattca
    23041 cgccgatgcg gtggcgatta ctaataccga tcatgtgctg gcctatgttg gcgtgggtga
    23101 acataactat cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta
    23161 cggaaaaatc atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat
    23221 gctggtgatc ccattgtggg agaaaggggt cgtgaccgga acgctgaaaa tttactactg
    23281 ccacgcgcat cagatcacct cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat
    23341 ctccacgcaa ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga
    23401 gattcgcgcc ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat
    23461 ttcatcgtca atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg
    23521 ttatctgcgc tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct
    23581 gtatcaaatt aaagactata ttgccattga gcaggcccgc tttggcgaca agctgacggt
    23641 tatctatgat attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt
    23701 ggtggagaac gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat
    23761 cagcgttgca gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat
    23821 cgttccaaag gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct
    23881 gctgaatgtc catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct
    23941 ggagccgggg acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca
    24001 ggctacgtta ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa
    24061 ttcctggcac aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc
    24121 ggcacctttg acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc
    24181 atttttctgg atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc
    24241 cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa
    24301 gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc
    24361 gggatgctgc aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc
    24421 gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc
    24481 acgccaatta acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg
    24541 cggcgtgaat cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg
    24601 tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc
    24661 gaaccgtggt ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg
    24721 gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc
    24781 atgaaagcac caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggccta
    24841 gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg
    24901 gtgggcagtt tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc
    24961 aacctgctgg aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca
    25021 cgcgcggcta atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca
    25081 ccgacctggc ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct
    25141 cgccagaccc agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc
    25201 acccatctta gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg
    25261 ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta attcacaacc gcgcggcgat
    25321 tttctctatc acagcatttg taactgggtt taggataatt atgcccagcc gctcacccgc
    25381 gagagcgtgg cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag
    25441 catggaacga tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg
    25501 attttgcaga aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat
    25561 agcgactatt tttgtcgcgt tttccggcgt cagtttagtc tgacgccggg agagtacagc
    25621 gcccgttttc agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt
    25681 tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc
    25741 agctgcgaga agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc
    25801 accagttcga cttcgcccat ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg
    25861 ctgatactgg aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca
    25921 acgccggtgg taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt
    25981 tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg
    26041 ctaaaatcct gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc
    26101 ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga
    26161 cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga
    26221 atacgcgggc tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa
    26281 cggctttcac cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg
    26341 gtaactattt gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat
    26401 acgcgcgggt tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata
    26461 ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc
    26521 accatgatcc caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg
    26581 atcgcttttt ggatctcgcc tttcacccat aagatctgat cgaggctgtg aaccatcggg
    26641 atcatcaact gggcgttgcc gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg
    26701 aacaggccag caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct
    26761 tcctggggaa tattcagata aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag
    26821 ataatcggct tgtcgcccgc cgccagtagc acctgctggt aggcttcaaa ttgctcctgc
    26881 tcgtccggcg cgctgtcgcg atccatatag agcatttcgg tacggaacag cccgacacct
    26941 tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata
    27001 tcaatacgct tgttatcacg ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt
    27061 tggcgtttat ccgccagcgt ctgcgcgacc tgataataac cgctcaccgc gtcattcggg
    27121 ttaatcgcca gcacgccgca ctgggcgtca agcactgcag gttgcccggc ataacgggca
    27181 atcgcatcca gcggcaagcc actcagtacc gggatcgccg aggcacgggc cagaatcagt
    27241 gtatgcgagg tgcgcccggt tttctccaga atcatacccg caagattttt caaatcaagg
    27301 ctcaaaaact ggcttggggt taaatcttca gccaccagaa tggtcggttt ttccagcacc
    27361 agattgttgc gcggcttcag ttccggccag gtgatatgca gcaactgttc gctgatatcg
    27421 cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag aagcagatag tttggcgcaa
    27481 acctgctcca tattgctgat gatcgccgcc cccagtccct gatgctgttc tgtcatcagg
    27541 cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgagc gctgaggata
    27601 gttttgcttt cgccgtcacg ctcacgcaat tgctgattca gttgctcggc aagcgttgcc
    27661 aggctgtgct ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg
    27721 aggctgtcgc tttgtaacag ggtcagcgta cccacgccga cgccgcttgc cagcacattg
    27781 ccgtacagta aatccgggtt aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt
    27841 tctgcctgcg tcggctgaac gctgtcgctg tcgataaagc gcaccaggat gtactcttcc
    27901 agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta
    27961 aataaggtgc cagttccaat caacgccagc gagcttttag catcagcctt tgcgttctgg
    28021 cgatggttga taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac
    28081 gccggacgag catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc
    28141 gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta
    28201 agacgttgaa taagggcaga taaaagttgc tccatctgca aaatgtcgcg gcaatcggca
    28261 atcgatgccg cgcaatgtcc gtggcgggtg gcaggcccca ttaccaatgt gggtacgcca
    28321 gtaccggtta agtgcaccgc tccaccgtcc gtgccgccgt tactgaacat atccgcctgc
    28381 aacggaacgc caatttctgc cgccacggtt tcaatccacg cggtaagttt tggcggcgca
    28441 atcagcgatt tatcgcttaa caccagcatc ggcccgttac caatctgacg atggttagcc
    28501 gcgccataat caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacgtccggc
    28561 gacaccgcgc gggtagcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc
    28621 accagccaca cttccgcagg cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc
    28681 agatagcaac tgaggcgatc atcaaaggct ttgcccatca cccgctggtg agggaggacc
    28741 tgaaaagtgg tatcgaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg
    28801 tcataggtgc gcgcgccaat gtccacgcgc atggcgctaa cgtcattccc ctgccggtcg
    28861 ccgtcaagca ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc
    28921 tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt caatagcgcc ttcgcgggag
    28981 atgctgcgca ccataaatcc cacttcgtcc atatgggcgc agatcatcac cttcggacct
    29041 gtcgattcat tgaggcggat cagcaccgat cccagaccat caaatcgcac ttctttttgc
    29101 aggcgagccg cttcttccag caggatctgc cgcacttcct gttccgagga ggcgatcgca
    29161 tctgcctcgc tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca
    29221 ctgttttcgg catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt
    29281 cgatgcgcac gcccccttgc cctagcaaat aaatccccgg ctccacggtc agcaacatgc
    29341 ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg acttcaatgc
    29401 cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa tccagcctcg gtaatgaccc
    29461 ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg gcgaattgca gagattgccg
    29521 cgagctgtgc ctgcaggaca atctgataga cgtcaaacag cgggtgagat tcggcgctca
    29581 ccccttcgcc attcaccagc aaggtgcgcg tcatatcagc agtagccctg atacagcgca
    29641 ccaaaatcga gagtgacaaa ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc
    29701 ggcagcgccc cgcgccagcc gctggcgaca atggtgtcaa aagaggcttt ttctgcgccc
    29761 tgctggcgca taaaccactc cagttcagcg gctatctcgc gctcgctcat ccccgcctga
    29821 ataaagcggc gaatatgctc cgctccgcga tcggcaatcc cacaggcgag gcggattatc
    29881 tccacctcct ctggcgtttt gatttgccgc agcacatccg gcgtggcgct aaccagtttc
    29941 gcattgagtt cagacttcca gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa
    30001 ccgagcgttt gcaactgctc atcggcaatg atttgattga cgatagtggt aagcgtgttc
    30061 gttgcgtcaa gcaaatgcag ctggtagcct tgcgcacggg cttccacctc aacgaaatag
    30121 cgcgaatcta ccagaatgtg cgcactttca cggctaataa ccacatagcc tgatccggtg
    30181 gagatcccca gatgcggctg tttgttctgc cgtgaggaga gaagcaccgc atccagttgt
    30241 tgcaccttaa gccagttgcg cagcgaagcg agtaatgtca tctggatttt ccttcttaca
    30301 ggctatcgat taacaatttg cctttgcgga acatcatcag acgcaggaac accaccatca
    30361 gcgcagtaat gattgccccc agcgcgatcc ccgccatata aacgcccaag ttagtgacca
    30421 gcggccatgc ccagatagcg gattccggga accattgcac tgcgcccagc cagacagctg
    30481 cggttgagcc gacaatcgcg ccgaccatat atgacggaat agcggtgatg gggctttcca
    30541 gcgcaaacgg aatcgcaccc tcactaatcc ccataaaggc caggaacatc gcggtcttac
    30601 cctgcggata aagttgagcg ttgaacaggc gtttgccggt taaacgacgg tcaataatgg
    30661 tcgccagacc cagaccaatc ggcggaataa cgatagcgat agaacgagcg gtgaccggta
    30721 atacgtggtc agtggtaaag ctgaaggcaa cgaaacctgc ggctttgttg atcgggccac
    30781 caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta catcagcgcg cccttctcac
    30841 ctgcggcagt cagcacagta cggataccgc cgttgatcca gccaccaaag ggagtgatga
    30901 cgtagtacat cgccagcatg acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt
    30961 tgaacgctaa caggaaatcc ggtaactgga ttttctggtt catccatttc acaaggtaac
    31021 cggcaacgat agaaataatc agcgcaccaa tgaaggtgga cggcaccggc gaagaggtcg
    31081 cccattgcat agtgctggga tcgaagttca gcagttgcgt cggctgggta gacatcaacc
    31141 cgccgataaa accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag
    31201 caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa
    31261 atttaagtaa ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc
    31321 cgatctcagc aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca
    31381 caccgcccat aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga
    31441 gttcacccca gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg
    31501 atgcgccagg cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg
    31561 aatattactg ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt
    31621 ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat
    31681 cttccggggt aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg
    31741 tcaggcgatt ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt
    31801 ctaccgccgc ttcttccagc acctgagcgg ccataaaagt gtgagccagg cccattgggc
    31861 aggcacataa ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac
    31921 tagtgaaatt atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg
    31981 gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag
    32041 aaaaattgaa ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac
    32101 aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag
    32161 acccggatag tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg
    32221 ccctttatct tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg
    32281 cacgataccg cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc
    32341 gccaaaacgg cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct
    32401 gtcagccagc gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc
    32461 agctttcaca attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc
    32521 cgaaacatga ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt
    32581 cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg
    32641 gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc
    32701 gaccggttct gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat
    32761 cgatacagcg gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat
    32821 ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg
    32881 gcaagcgatg gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg
    32941 aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc
    33001 accactggca atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa
    33061 tgcatacttt gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg
    33121 taaatacctg ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct
    33181 gggtcacaca attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg
    33241 ggcaaactaa gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa
    33301 ccatgttgtt attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga
    33361 ttgtggtgat gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa
    33421 ctctggggat agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta
    33481 ttgcggtggg gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg
    33541 aaccgctgat cggcgcaccg gttccgccct ctgcgctacc tagacttatc gtagcattaa
    33601 ttctcggtct tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata
    33661 tcgcccttgc cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga
    33721 ctattttagc ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt
    33781 tgatattttc gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg
    33841 cgccgttaat ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt
    33901 tttcactgcc cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa
    33961 ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc
    34021 acgcgatgat gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc
    34081 tcggaattct gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc
    34141 agcagatggt ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta
    34201 aacttgccgc cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg
    34261 cagtgtttgt cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag
    34321 cggcaataac cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct
    34381 ggttaagtct ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct
    34441 gtatcgtcat gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc
    34501 gtccgaagca acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt
    34561 aagagttatg gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct
    34621 gacggcttcg atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc
    34681 tgatggcatt taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca
    34741 aattacaaat tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag
    34801 atcgtgatta ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata
    34861 atggctatca tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct
    34921 ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc
    34981 gtcctccaat ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac
    35041 cggctcttta acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga
    35101 tgcgacgctc attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg
    35161 ccacgaccag cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat
    35221 cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc agggcgatac
    35281 caaaacttaa cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa
    35341 tgacaataaa tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc
    35401 gaatgaatcc ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc
    35461 cggcagcaac cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca
    35521 gcgtcagata agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg
    35581 ccgcagctgt agccatcatc accagattga caaaaccggc aatcgtcatg gcgatagcca
    35641 catcccattt ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga
    35701 gcgaagagtg caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca
    35761 ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca
    35821 ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc
    35881 cgccaatcac tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag
    35941 tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac
    36001 cgatcgccgc accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa
    36061 cccaatagaa ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt
    36121 taccggtggc aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg
    36181 cccaaacgac aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg
    36241 caaagttacc gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga
    36301 gcctcatctt acgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt
    36361 gcctctaaaa catagccttt gctatgtttc atgctatgct aaacgagaat gattatcaaa
    36421 ttcatttaaa tggattgtgg tgatttctct gatagaccaa gattatgatt acgaaaagat
    36481 tgatgacttg aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc
    36541 catacacaac atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg
    36601 tcattatagt gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga
    36661 aatttaacct gcctcatatt tggagcaaat atggaccgcg tccttcattt tgtactggca
    36721 cttgccgttg ttgtgattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc
    36781 cgttatgtta ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc
    36841 gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt
    36901 gccaacgaag ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc
    36961 ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac
    37021 attcgcgtgt tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt
    37081 atgggcaaac tggaatcctt taacgccgtc agctccctga ttctgggtca gtcagaaaac
    37141 tttattgcct ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct
    37201 gccacggcaa tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa
    37261 ccgaaatacg tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg
    37321 ctgatcaatc cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac
    37381 gaaggtcaga gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc
    37441 attatcgttg ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg
    37501 tttgccaccg ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac
    37561 atcttctatc cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc
    37621 agtatcatgg cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa
    37681 attgcttcca cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct agtttccttc
    37741 gctaacttct cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa
    37801 ggtaacgtgg tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg
    37861 ctgtctgcgt caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca
    37921 ttccccggct tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc
    37981 cttgcagata ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa
    38041 actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca
    38101 tcgcatccag cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat
    38161 cagcctgcaa acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg
    38221 caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag
    38281 aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct
    38341 taaacagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat
    38401 tgactgaaaa gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg
    38461 attccagcac ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa
    38521 acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac
    38581 cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg
    38641 ccgtgcgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct
    38701 ctctgctatc cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta
    38761 actgccccaa caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac
    38821 catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa
    38881 ccatatgctg gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac
    38941 ccggcaattg atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg
    39001 agcggataca gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat
    39061 caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac
    39121 gaaagttggg taggagcgtt aacggatctg ttagcgcctg caaatgccag cggcgattaa
    39181 gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca
    39241 gcaccgcgag aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctgat
    39301 tgtaattcag aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa
    39361 gattaccgac cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa
    39421 agtcgttttc ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc
    39481 ttaaggtaaa tgcgagtgag atagtagaaa agcatgttgt aaatcagcac tgcggtgaaa
    39541 aggcttagta aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc
    39601 tttagcggga aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc
    39661 gtcaccaaac caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc
    39721 caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg
    39781 ctgccgagca tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa
    39841 aacatcccgc ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc
    39901 attacgcaca gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc
    39961 gaaataaaac gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc
    40021 ttgatatttt ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg
    40081 gttttcaggc acacaaataa taagtttatt cgcatcgcga agcaaacagg ggccagcctg
    40141 ttgccgattt ttcatccaat gatgctttca ccaggcggcg ctaacccgga cgtagactgt
    40201 attacaaaag cggcaaaaag cagagacaaa aaacccccgc tttgcagcga gggttggaaa
    40261 tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc
    40321 cagctgagct atagccccac gatgcgttta cgtaccaaat ttgctgggtg caaaatttgg
    40381 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    40441 gagctatagc cccgtcacgt aaagcttgtc gagttgacgg gcggcatcat atgaattccg
    40501 cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa tcgctgaaaa atcaggcaaa
    40561 tgaacaattt tggaaaaccg ctcgcattca gtagttattc atgtcacggt ttcctgtaaa
    40621 gtggtgttat aaaatgaact actaatagac ccacatacat tcagggaatt gttatgttca
    40681 aggagaggat gacaccagat gaacttgcca gactgaccgg ctatagccgc cagaccatta
    40741 ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa acctggcgtc cagggtggca
    40801 aagcccgact ggttcacgtc aatgaacagg ttcgtgaata tattcgcaat gctgaacgtc
    40861 cagaaggtca gggagaagcg cctgcccttt ctggtgatgc gccgcttgaa gtattgctgg
    40921 tgacactggc gaaagagatg acgccagttg aacaaaaaca gtttacatcc ttgcttctgc
    40981 gggaagggat tatcggattg ttacaacgct tagggattcg cgatagcaaa taatatgaaa
    41041 agattacgca ataaaatgac caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa
    41101 actgttaacc gttggaccag agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa
    41161 ggcggtcgtg ccagacttat tctggtcgat acgcaagttt gcgagtttat tcagaacacg
    41221 ccagccttcc ataacacacc aatgttgatg gaagctgaag agagaattgc tgaatacgct
    41281 cctggagcac gcgccccggc gtatcgacaa atcatcaacg cgatagataa tatgacagac
    41341 attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag gaattcgcaa tttcctcgcc
    41401 cgtctcgata ttgacgaatc agcataaata aaaaacggca ggatattatc tcctgccgtt
    41461 tatcttttta cacgctaatt actgctgatt ttcgcgttca gcaataaaat ccagcgcttt
    41521 gttgatacgc tcgatactgc gggtcttacc aattgcgtga acggtaacat ccagtgctgg
    41581 agactgcccc gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac
    41641 ttccagctca tccgccgtcg cctgaatagc gtgatgaacg ttttcagcgg tccagtaagt
    41701 aatcgcggcc agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg
    41761 ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg
    41821 tgccatctct ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat cagccagctg
    41881 cgggccgtta cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagcaac
    41941 atactccggc ggcagcgcgt taatgtagtg atggttcagc cacagcagct tgtcggtgtt
    42001 gaacgcactg gcagatttgc tgacggcatt caaagtgaag tatttgatca tctcttcacg
    42061 agtgaagatt tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc
    42121 ttctggcaaa taaccgtcat cacgatactg cattacgctg actgccccgt gacgtttgga
    42181 cagtttttta ccgtcatcgc cattgatcat agaaacgtgc gcgtaaaccg gcaccggcgc
    42241 tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg ttgatatggt cttcgccacg
    42301 gataacgtgg gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg
    42361 ggaaccatcg gtacggcgga taataagatc gtccagttcc tggttgctga actcgatcgg
    42421 accacggatc tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacaac
    42481 acacggttca tcatcagcat gatgctcatg gctgtggcgg cagcgaccgt cataacgcgg
    42541 cttctcacct ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata
    42601 gcatttataa gcagtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa
    42661 acgtttggtc tggtagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc
    42721 cataatggct tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag
    42781 cacgaactca ccgccgtggt tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc
    42841 gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg
    42901 gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata
    42961 ttctatcact cccgcctgat tcctcaatgt agttcgggtt tctaccttac gcattattgt
    43021 tttcgttgac aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac
    43081 gaacgaacaa tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac
    43141 tccacacagc gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
    43201 ggcggttcga tcccgtcatc acccaccaac tactttatgt agtctccgcc gtgtagcaag
    43261 aaattgagaa gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
    43321 ggcggttcga tcccgtcatc acccaccact tcgggtcgtt agctcagttg gtagagcagt
    43381 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccaat gtaaaaaagc
    43441 gccctaaagg cgcttttttg ctatctgcga tactcaaaga ttcgaacctg cagcaggttt
    43501 gagttgagcg cagcgaaaca acggagccgc tcgcggcgac ggcccgaagg gcgagcgaag
    43561 cgagtcatcc tgcacgaccc accaatgcaa aaaagcgccc taaaggcgct tttttgctat
    43621 ctgcgatttg taagattgat ttttcttctc aagttccttg ttcttctacc gatattacct
    43681 cgtacagtga cgaggactga acaatggcca ctatctcttc aacttcaatt ccttccatac
    43741 aaacacagag tagtaacagg gcatcacaag gtagcgatgt tgcttcacaa atcgcgcgga
    43801 taagtcagca aatcatcaaa ctcactcaac aaattaaaga gattgtagac actagcggca
    43861 gtgctgaaga caagcaaaaa caggccgaat tgattcaaca gcaaatcacc ctactggaaa
    43921 cacaactggc gcagttgcag aaacagcagg cagaaaaggc gcaggagaaa gagcagcgtt
    43981 tgtctctgaa tgtatccctt ctaaatcctg ttgaaaaaac gactcatatc gatatctata
    44041 tctgatacaa cggatcccct tcccgaccag ccattagcgc attgccctgg tctcgtacca
    44101 cgtcccataa cgcttcagcg gcggtggaaa gtgaacggtt cttcctgcgc accagcatca
    44161 actggcgttc aaccaccggc gtaatgcgct taaccaccag tgggctacct tcaggcaatg
    44221 gtagcgccag tgcaggaaga atactgatgc cgatgcctgc ggctaccatc gggaacagcg
    44281 tcgccggatg tccaatctcc tgcacaatat tcgcctgaat accattgcgc gccagcgctg
    44341 cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa caccaatttc gctccctgta
    44401 atgcctgcca gggtacataa tcttccacgg ccaaagcgct atcgcgatgg caaagcagaa
    44461 agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc cactgggcct ggatcgatga
    44521 cgatgccaaa gtcgacatct ccctggcgaa tactttccat cacccactgc tgcggacgat
    44581 catgcaggac aaactgaata tctggatagc ggcgatggct ttcagcaatg cattgcggaa
    44641 taagatgcgc ggaaatggtc tggctggcag cgacccgaac ttttccgctc agttgttgcc
    44701 ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc cagcagtcgc tcaagacgca
    44761 aagccaactg ctgccctgca tctgtaagca ccacttcacg cgtggttctg tccagcaggc
    44821 gaacaccagt atgattttcc agctccttca cactgtgact tactgccgac tggctcaagc
    44881 cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta
    44941 attgttttaa agaataattc atctgttttc ttcatgaatg gatgcaataa atcaatttta
    45001 tttctcaaac tggaagaagc acaatagaac catcgatcat ctggagtctt tatgaaactt
    45061 tttcgtatcc tcgatccttt caccttaacc ctgatcacgg tggtgttgct ggcctctttc
    45121 tttccggcca gaggcgattt cgtccccttc tttgaaaatc tgaccaccgc agctattgcc
    45181 ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt
    45241 cactggcgac tgcatttgtg ggtaatgtgc agcaccttcg tgctgtttcc gattctgggt
    45301 gtactgtttg cctggtggaa accggtaaat gtcgacccga tgctctactc cggttttctc
    45361 tacttgtgca ttctcccggc taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc
    45421 ggtaacgtcg cggcggcggt ttgttctgcg tcagcatcca gcctgctggg gattttcctt
    45481 tcaccattgc tggttggtct ggtgatgaat gttcacggtg caaggggcag ccttgagcag
    45541 gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt tggggcatct ttcccggccg
    45601 tggattggtg actgggtgtc gcgcaataaa aaatggattg cgaaaactga ccagacgtcc
    45661 attctgttgg tggtttatac agcgttcagc gaagccgtcg ttaatggtat ctggcataaa
    45721 gttggctggg gatcattgct gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg
    45781 attgtagtta acgtctttat ggcacgccga ctgagcttca ataaggcaga tgaaattact
    45841 atcgtctttt gtggttcgaa aaagagtctg gcaaatggca tcccgatggc aaacattctg
    45901 ttccccacat cggtgatcgg tatgatggtg ctgcccctga tgattttcca tcagatccaa
    45961 ttgatggtct gtgcggtgct ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg
    46021 cagcaggaaa gcagcgccga taaagcttaa agcggacgct tcaggggctg gaccagttgc
    46081 gtcagcccct cggttttaat cagcagcgtg atttgcatta gctcaccgag cttccctgcc
    46141 gggaactcat ctttgcgggc aaaccacagc agatactcct ccggcaggtc aattaagcga
    46201 cgccctttgt atttgccaaa cggcattatc gtattggcta tttcaatcag ctgctctttt
    46261 tccatctcag ctacccagca aacgcagcat ttccgcttcg tcgatgactt caatgcccag
    46321 ttcctgcgcc ttcgccagtt tagatcctgc agcttcaccc gctatcacca gatcggtttt
    46381 cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc
    46441 acgcgacatc tggcttaagc tgcccgtaag caccacggtt ttaccagcaa acgggctgtc
    46501 aatctcttcc gcgttgataa cgatcggcgc aggccagtga acaccttccg ccaacagctc
    46561 gctgatgaca ttgcggttgc tttcttcggc aaagaagttg tgaacgtggg atgcaacgac
    46621 aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag
    46681 cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg ccgacttcac ggatgccaag
    46741 tgcatagagg aagcgagcaa aggtggtttc tttcgctttt tccagcgcgt taaccacgtt
    46801 ttgtgccaat tttggcccca tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa
    46861 cagatctgcc ggagtatgga catattcttt ttcaaccagc tgatcgatga ttttgtcgcc
    46921 cattccgtca acatccatcg cacggcggga aacaaagtgt ttcagcgact ctttacgctg
    46981 cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct tcaccttcca cacgctcaac
    47041 gtcagaacca cataccggac aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg
    47101 ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac
    47161 tttatcgcca atgcgtaaac caagacgttc gatttcatcc gcattgtgta aggttgcgtt
    47221 actcaccagc acgccagcaa catggacagg ttccagacgc gcaacaggcg taatcgcgcc
    47281 agtacgccca acctgaaact cgacgtcacg cacaaaggtc atctgctcct gcgccgggaa
    47341 tttaaacgca accgcccagc gcggggcacg cgcgacaaag ccaagctgct cctgctgttc
    47401 cagtgagttg accttaatca ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc
    47461 ttccactttg tgatagaacg ccagcacttc ttccgccgat tcacaaagcg ttacccgatc
    47521 gctgaccggc accccccact ttttaaattg cagtaaacgg ccaagatgag tatccggcag
    47581 ctcgccacct tccagaacac caacgccata gcagaaaaaa gtgagcggtc gcttcgctgt
    47641 aatacgcgga tcaagctgac gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac
    47701 tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg aaccccgcct gcggcaggaa
    47761 cacttcacca cgcacttcca gacgcgccgg gatattctct ccgtgcagct tcagcggaat
    47821 ggcgcgaata gtacgcacat tagacgtgat atcttccccg gtggtgccat cgccacgggt
    47881 cgcggcactg actaaaacgc cattttcata cagaatactg acggcaaggc catccagctt
    47941 cagctcacag caccaggtga ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt
    48001 gaaagcaaga aagctttctt catcaaaaac gttatccagt gacagcattg gtacttcatg
    48061 gcgtatctgg ctgaaagctg ccagcggcgc agcacctaca cgttgggtag gcgaatcagg
    48121 cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt tcgcgcatca gcctgtcgta
    48181 ttcagcgtcg ggaatttccg gcgcatccat cacatgataa agatattcat gatggcaaag
    48241 cgtcgttcgc agttctgtca gttgttgttc gattgattcc atatcgcacc atcaatgcta
    48301 aaaacccccg acaagcgggg gttcgaagag gagttaattt gccttaagtg tatcaggcgt
    48361 tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg caatttctgc ggagtcatca
    48421 tacggcgctg atcgtcaagc acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt
    48481 gcagcatcag cttgaagttc tgcagctcgt caccgtaaga cggtacctgc ataaagatag
    48541 tgacacccgg agtagtgaaa tccttcattt caggatcaaa ggttcccggt ttcaccatat
    48601 tcgccaggct gaataacgcc gggccgctgc catccgggct aagatgacga tggtaaatat
    48661 tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta
    48721 gctcgctacc gtgatgcgcc gcgacgttca tgataatcac cgcttctttg cgcttcggtt
    48781 tatccataac tggagccggt tccgctacag gctcaggctg tggtgccgct acgggttctg
    48841 caggctggaa agcctgttgt gccggttgcg gtgctgaatg cacaggctgc ggcgctggcg
    48901 cgacctgtgg cgaaactggc tgctgcaacg gctgttcagg ctgcggctga taggcaggct
    48961 gctgcaccgg ctgcgctgga cgcggagcat gttgcggcgg tacctgcgct tcaggcggct
    49021 gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg taggtactgg tgttgcggcg
    49081 acggacgagc agcctcatgc tcctgagcgt tagccggggc atgattcacg cggtgaacac
    49141 gaacctcacc aacgccctca tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt
    49201 ttgacttcat tcgttttaat ggccgatcgc ggaacataga agatcgttct ttacggctgg
    49261 tccagaaacc atgtaccagt aaagcgatta tggcgatcgc gccaacaatg attaatatca
    49321 gacgcaaatc ctgcatcatt atattctctg ttgttctaac accttgccac cacggcaaac
    49381 atttactcac taagagtatt tgccgattac ctcaagtgca agtgcactat taactttcac
    49441 agcacaaaga tagatgaaat cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc
    49501 attgcgcaat tacccggtaa aatacgcaga attttcctgg gattggtcaa aaggagctca
    49561 tcctgattat ggtttcatca ttcacatctg ccccacgcag cggtttttac tattttgcgc
    49621 aaggctggaa gctcgtctcg caacctggga ttcggcgttt cgttatttta ccgctgctgg
    49681 tcaatatttt gttgatgggg ggcgcattct ggtggctctt tacacagctc gatgtctgga
    49741 tcccgacttt catgagttac gttccggact ggctacaatg gctgagttat ctgttgtggc
    49801 ctctggctgt catctctgtg ctgttagtgt ttggctattt cttctccacg attgctaact
    49861 ggattgccgc tccgtttaac ggtttattgg ctgaacaact ggaagcacga ttgactggcg
    49921 ctacaccgcc agataccggg attttcggta tcatgaaaga tgtgccgcga atcatgaaac
    49981 gcgaatggca aaaatttgtc tggtatctgc cgcgcgcaat tgtattacta attctttact
    50041 tcatccctgg tattgggcaa accgtcgcgc cggtactgtg gttcctgttt agcgcctgga
    50101 tgttagccat ccagtattgc gattacccct tcgataacca caaagtgccg tttaaagaga
    50161 tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc
    50221 tgtttacgat gatcccgctg cttaatctgt tcatcatgcc cgttgccgtt tgtggcgcga
    50281 cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat gtggcggtaa caatctaccg
    50341 gttattttgt aaaccgtttg tgtgaaacag gggtggctta tgccgcccct tattccatct
    50401 tgcatgtcat tatttccctt ctgtatatag atatgctaaa tccttacttc cgcatattct
    50461 ctgagcgggt atgctacctg ttgtatccca atttcataca gttaaggaca ggccatgagt
    50521 aagatttttg aagataactc gctgactatc ggtcacacgc cgctggttcg cctgaatcgc
    50581 atcggtaacg gacgcattct ggcgaaggtg gaatctcgta accccagctt cagcgttaag
    50641 tgccgtatcg gtgccaacat gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc
    50701 gttgaactgg ttgaaccgac cagcggtaat accgggattg cactggccta tgtagctgcc
    50761 gctcgcggtt acaaactcac cctgaccatg ccagaaacca tgagtattga acgccgcaag
    50821 ctgctgaaag cgttaggtgc aaacctggtg ctgacggaag gtgctaaagg catgaaaggc
    50881 gcaatccaaa aagcagaaga aattgtcgcc agcaatccag agaaatacct gctgctgcaa
    50941 caattcagca atccggcaaa ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg
    51001 gaagataccg acggtcaggt tgatgtattt attgctggcg ttgggactgg cggtacgctg
    51061 actggcgtca gccgctacat taaaggcacc aaaggcaaga ccgatcttat ctctgtcgcc
    51121 gttgagccaa ccgattctcc agttatcgcc caggtgctgg caggtgaaga gattaaacct
    51181 ggcccgcata aaattcaggg tattggcgct ggttttatcc cggctaacct cgatctcaag
    51241 ctggtcgata aagtcattgg catcaccaat gaagaagcca tttctaccgc ccgtcgtcta
    51301 atggaagaag aaggtattct tgcaggtatc tcttctggag cagctgttgc cgcagcgttg
    51361 aaactacaag aagatgaaag ctttaccaac aagaatattg tggttattct accatcatcg
    51421 ggtgagcgtt atttaagcac cgcattgttt gccgatctct tcactgagaa agaattgcaa
    51481 cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg
    51541 cctgcttcaa actttcgccc ctcctggcat tgattcagcc tgtcggaact ggtatttaac
    51601 cagactaatt attttgatgc gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga
    51661 ttttatgatt tggttcaatt cttcctttag cggcataatg tttaatgacg tacgaaacgt
    51721 cagcggtcaa cacccgccag caatggactg tattgcgctc ttcgtgcgtc gcgtctgtta
    51781 aaaactggcg ctaacaatac aggctaaagt cgaaccgcca ggctagactt tagttccaca
    51841 acactaaacc tataagttgg ggaaatacaa tgttccagca agaagttacc attaccgctc
    51901 cgaacggtct gcacacccgc cctgctgccc agtttgtaaa agaagctaag ggcttcactt
    51961 ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc
    52021 agactctggg cctgactcaa ggtaccgttg tgactatctc cgcagaaggc gaagacgagc
    52081 agaaagcggt tgaacatctg gttaaactga tggcggaact cgagtaattt cccgggttct
    52141 tttaaaaatc agtcacaagt aaggtagggt tatgatttca ggcattttag catccccggg
    52201 tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa attgtcattg accggaaaaa
    52261 aatttctgcc gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc
    52321 atcagcccag ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga
    52381 agccatcttt gaagggcata ttatgctgct cgaagatgag gagctggagc aggaaatcat
    52441 agccctgatt aaagataaac acatgacagc tgacgcagct gctcatgaag ttatcgaagg
    52501 tcaggcttct gccctggaag agctggatga tgaatacctg aaagaacgtg cggctgacgt
    52561 acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta tcgacctgag
    52621 cgccattcag gatgaagtca ttctggttgc cgctgacctg acgccgtccg aaaccgcaca
    52681 gctgaacctg aagaaggtgc tgggtttcat caccgacgcg ggtggccgta cttcccacac
    52741 ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc
    52801 tcaggtgaaa aatgacgact atctgattct ggatgccgta aataatcagg tttacgtcaa
    52861 tccaaccaac gaagttattg ataaaatgcg cgctgttcag gagcaagtgg cttctgaaaa
    52921 agcagagctt gctaaactga aagatctgcc agctattacg ctggacggtc accaggtaga
    52981 agtatgcgct aacattggta cggttcgtga cgttgaaggt gcagagcgta acggcgctga
    53041 aggcgttggt ctgtatcgta ctgagttcct gttcatggac cgcgacgcgc tgcccactga
    53101 agaagaacag tttgctgctt acaaagcagt ggctgaagcg tgtggctcgc aggcggttat
    53161 cgttcgtacc atggacatcg gcggcgacaa agagctgcca tacatgaact tcccgaaaga
    53221 agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg atggatcgta aagagatcct
    53281 gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt
    53341 cccgatgatc atctctgttg aagaagtgcg tgcactgcgc aaagagatcg aaatctacaa
    53401 acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt
    53461 ggaaacaccg gctgccgcaa caattgcacg tcatttagcc aaagaagttg atttctttag
    53521 tatcggcacc aatgatttaa cgcagtacac tctggcagtt gaccgtggta atgatatgat
    53581 ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga
    53641 tgcttctcat gctgaaggca aatggactgg catgtgtggt gagcttgctg gcgatgaacg
    53701 tgctacactt ctgttgctgg ggatgggtct ggacgaattc tctatgagcg ccatttctat
    53761 cccgcgcatt aagaagatta tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga
    53821 gcaggctctt gctcaaccga caacggacga gttaatgacg ctggttaaca agttcattga
    53881 agaaaaaaca atctgctaat ccacgagatg cggcccaatt tactgcttag gagaagatca
    53941 tgggtttgtt cgataaactg aaatctctgg tttccgacga caaaaaggat accggaacta
    54001 ttgagatcat tgctccgctc tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg
    54061 tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa accaacgggt aacaaaatgg
    54121 tcgcgcctgt agacggtacc attggtaaaa tctttgaaac caatcacgcg ttctctatcg
    54181 aatctgatag cggcgttgaa ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag
    54241 gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt gaaagttggc gatactgtca
    54301 ttgaatttga tctgccgctg ctggaagaga aagccaagtc taccctgact ccggttgtta
    54361 tctccaacat ggacgaaatc aaagaactga tcaaactgtc cggtagcgta accgtgggtg
    54421 aaaccccggt tatccgcatc aagaagtaat tcttgccgca gtgaaaaatg gcgcccatcg
    54481 gcgccatttt ttatgcttcc gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct
    54541 gggtgtagcg catcacttcc agtacgcgca accccgcacg gtgcaccgca tcgtttaacg
    54601 ccttcccttt cagcaaacca ctgataagct gagcacaaaa caggtcgcca gtccctttca
    54661 ggtcggtttt tacccgtgaa tgggaaatga cattcacgct gtcggcactg accaccacca
    54721 cctgcatctc ctgattttct tcattaccgg aggcgctggt aatcaccacc cattttaatg
    54781 tgtctgaaag cagacttttt gcggcagcaa tggcactgtc gagatcgcgg caatttttac
    54841 cggtcaggat ttccagctca aagatattgg gggtaattcc ctgcgccagc ggcagtaaat
    54901 attgtcgata cgcctcggga aggtcaggtt tgacataaat tccgctatca atatcgccaa
    54961 tcaccggatc gaccatgatc aataggtcag gatggtcttt gcgtagcgca gtcagccact
    55021 cggcaaggat tttgatttgc gatgccgttc ccatatagcc cgtggttaca gcacgaagtt
    55081 ggcgcagcgc atcacgctcc tgaagcgcac gcaaatagcc gctaaaccat tcgtccggaa
    55141 tcgcaccacc gtagaaagtg tcataatgcg gcgtattgct cagcaatacc gtcggcacgg
    55201 caaagacatt caagccgttc tgttttatag caggcaccgc aatgctgttg cctacgcttc
    55261 cgtacactac ctgcgactgc acggcgacga tatccgcctg cagcgcccta ctcttatcgt
    55321 taaacaacaa caaactactc atttaatttt ttctccttgc cgatgatcct catcgtattc
    55381 caaccgaaac tttacctgat tctggcagtc aaatcggcta ttacaaaaca aagataaggt
    55441 aattcagtga agaaaatcat ttgtctggcc attacgctgc taatgacact ccccgcttac
    55501 gcgaagttaa ctgttcatga agaagcccgc atcaacgcca tgctggaggg attagcacag
    55561 aaaaaggatt tgatatttgt gcgcaacggt gatgaacata cctgcgatga agcggtttcc
    55621 cacctgcgtc tgaagctcgg caatacccgt aaccgcattg acactgccga gcagtttatt
    55681 gataaggtag cttcgtcgtc atcgattacc gggaagccgt atatcgtgaa gatccccggt
    55741 aagagcgatg agaacgcaca gcctttttta catgcgttaa ttgcgcagac ggataaaacg
    55801 gtgcctgcgg aaggaaatta atccgctttg gtaaggcatt tacaggaggt aacatgaaaa
    55861 aatgctttat ttatcacgat gaaaaatcga ataaattttg gtggatagat tacgaagggg
    55921 atagtttagc tgtcaactat ggcaaggtag gtagtattgg taaattccat acaaaagaat
    55981 tcgataatga agaacagtgt ctgaaagaag ccagtaaatt gattgccgca aaaatgaaaa
    56041 aaggatatca agaaactcca gactttaact tcatggatct ctactatttt gatgatgaag
    56101 agattgggtt acatcttaaa acgtcacacc caaacttcca gtgccatttt actgatccac
    56161 tttatatgtg ttgctgggat gaagaatctc cttttggtag cgatgaaggt gctgatgctc
    56221 taaacgaact tgaaaatagc ctccgttctg gactgtgctg atttccctca aatgttaatt
    56281 gaaactatgt ggggtatgaa atacatcgct atggacagta ttcttgaaga ggatgtgcgt
    56341 gcgcaattac tagccgatga aatgagctct atccagagca atatgattac ctacgcgact
    56401 gcattcggtc agatcaaagt catgggtaaa atctcccata aacttaaaaa gatgggactc
    56461 aatgcactag cgcgtcatca gcttaccgca aaaattcttc aatggggtga cggtcaggac
    56521 tcaccaatac ttcaaaaaat gattgatgac cttactgctt ttccacacga aaattaaaaa
    56581 ctgcatttgt cggcagcaac aactgccgac aaatcagatt attgacaaag tgcactttgt
    56641 ttatgccgga tgcggcgtaa acgcctgatc cggcataaaa atattgctaa ttcaatatat
    56701 tgcagaaaca ttgtaggcct gataagcgga gcgcatcagg cagttttgcg tttgtcatca
    56761 gtctccaatg ctattaatcc ttaaatccct gccccctgac taaaatgctc ttccccaaac
    56821 actccggtgg agaggtagcg atcgccacga tcgcagatga tcgctaccac caccgcacca
    56881 gggttagctt ttgccacccg cagtgctcca gcaaccgcgc cgcctgagct gacgccacag
    56941 aatattcctt cccgcaccgc cagttcgcgc atggtgtttt ccgcatcgcg ctgatgaata
    57001 tccagcacct catccaccag cgcagcgttg aaaatccccg gcagatattc cgcaggccag
    57061 cggcgaatgc ccggaatact gctgccttct tccggttgca ggccaacaat ggtcaccggt
    57121 ttggattgtt cgcgcataaa gcatgaaacg ccggtgatag tgccggtcgt ccccatgctg
    57181 gagacaaaat gagtgatgcg cccgccggtt tgctgccaga tttccggccc ggtggtggtg
    57241 taatgcgcat aagggttatc gggattattg aactgatcga gcagctttcc ttcgccacga
    57301 ttcgccatct ccagcgccag atcgcgcgca ccttccatgc cctgctcttt ggtgacaaga
    57361 atcagttccg caccataagc acgcatcgcc gcacggcgtt cctggctcat gttgtcgggc
    57421 atcagcaatt tcatgcgata gcctttcagc gcggcaatca ttgccagcgc aatgccggtg
    57481 ttaccactgg tggcttcgat taagacatca cccggtttaa tttccccgcg cttttccgcc
    57541 tcgacgatca tcgaaagtgc cgcacgatct ttcaccgaac ctgccgggtt attgccttcc
    57601 agttttaacc acacttcact gccgttatcc ggccccattc gctgcaactt caccagaggc
    57661 gtattgccta ttgtttgttc taatgtactc acaatctcta tccatacgtg gtgttgcctg
    57721 atgcgacgct tgcgcgtctt atcaggccta caggttacaa accttgccat taaaaagccc
    57781 ggatcgcggg aagcgcctcc gggcgtttaa cattcactca acctatcagg cgctttgtgc
    57841 gagagcaagt tcctcatcgc gggtttcaat acgctcgtcg ccgttataca gccgcgcatg
    57901 ttgcagacca acgaataaac gctcgccatg ctgcggggca tcgtcgccat gcatcacgac
    57961 cgtcagcggt tcgttgtacc accccagcgg ctgcaccact aattgggtgt agtgaccttt
    58021 cgggctggct tccagtacct gtaccggcag cggcgaatcg agactggtac ggcggctgat
    58081 atccacttcc cacgggcgca ggaagagatc caccggcccc tgatacgcag gtgtgtagcc
    58141 cagcggccag cgatgcgcgc caacatggaa ctgcccgccg cgaatggttc cctgcaggcg
    58201 gttcacttcg cccataaatt cgagtacaaa acgggtcgct ggttcgcgcc atacctgatt
    58261 cggcgcatca gcctgttcaa tattcccctg actcatcacc actacacgat cggctacttc
    58321 ggtcgcctct tcctggtcgt gggtcacaaa cacgctggtg aatttcagct cttcatgcag
    58381 ttgacgcagc cagcgacgca gctctttacg cacctgcgca tccagcgcgc cgaacggttc
    58441 atcaagcagc agaatttgcg gttctacagc cagcgcgcgc gccagcgcca cacgctgttt
    58501 ctggccgccg gaaagctgcg ccggataacg atccgccaga tgcgcaagct ggaccatttc
    58561 cagcaatttt gtcactttcg ctttgatggc tgcggcattc gggcgctcgc gacgcggcag
    58621 caccgtcagg ccaaaagtga tattgtcgaa taccgtcata tggcggaaca gcgcgtaatg
    58681 ctggaacacg aaaccgactt tacgatcgcg cgcgtgcagg cgactcacgt cggtgccgtg
    58741 gaagcgaata tgcccgctgg tttgatgctc cagcccggcg ataatccgca gcagcgtggt
    58801 tttcccggaa ccggacggcc ccagcaacgc gaccatctga ccagaaggaa tatccagtga
    58861 gatatcgttc agcacctggg tgcgaccaaa agacttcttg atattggcaa tctcaatgct
    58921 catgatgctc ctcctgctgc gcgcgttttt cctgattctc caggcgccac tgcaacatac
    58981 tctttaaaaa cagggtgata atcgccatca acgtcaacag cgcggcggcg gtaaaggagc
    59041 cgacggtgtt gtagtcctgc tccaacaatt caatctgtaa cggcagcgaa agtgtttcgc
    59101 cgcgaatcga gccggaaacc accgacactg caccaaactc gccaatcgcg cgggcgttgg
    59161 tcagcaccac gccgtaaagc agcgcccagc gaatattcgg caatgtgacg cgacggaaca
    59221 tctgccagcc ggacgcgcca agcaaaatcg ccgcttcgtc ttcctggctg ccctggctta
    59281 acatcaccgg caccagctcg cgcaccacaa acggacacgt cacaaagatg gtgaccagca
    59341 ccattccggg ccaggagaac ataatttgca gattatgttc atccagccag ccgccgagcg
    59401 ggccgttgga gccgtagaac agcagataca ccagaccggc aaccaccggc gatacagcaa
    59461 acggaatgtc cagtagcgtc agcagtaact ggcgtcccgg gaagttaaag cgcgtcacca
    59521 gccaggccag cagaatgccg aacaccaggt ttaccggtac ggcaatcagc gcgatcatca
    59581 ccgtcagcca gatggcgtgc agcatgtccg gatcggccag attctgtaaa accggcatca
    59641 gccccttgct gaatgcctgc acgaagatgt aaatcatcgg caccagcagg atgaacgccg
    59701 aaaccagcat cccgatgcca atcagaaacc atttgcccca gttaatcggg cgcgcgtcat
    59761 aacgcttcaa ttgggtaact tccgccatta atgacctacc acacgccgac caaagcgact
    59821 ttgcagagtg ttaattgaga acagcagcag cagagatgcc gcgaggatca ccgaagcaat
    59881 cgcgctcgct gccgggtaat caaactcctg taagcgcaca aaaatcatca gcgacgtcac
    59941 ttccgtcttc cacgcgatat ttccggcgat aaaaatcacc gcgccaaatt caccaagact
    60001 acgggtaaac gacagcgcca cgcccgccac cagcgccgga gaaagctccg gcagcaccac
    60061 tttgcagaaa ctctgccagc gcgttgcacc aagcgtttcc gccgcttctt catattccgg
    60121 gcctaactct tccagcaccg gctgcacggt acgcaccaca aacggaatgc tggtaaaggc
    60181 catcgccacc gcaataccaa gccaggtata ggtgactttg atatcaaact tcgccagcca
    60241 ttcaccgtaa aaaccgttta cggaaaagag cgaggccagc gttaaaccgg cgactgccgt
    60301 tggcagcgca aagggtaaat ccatcagcgc atcaagcagc gtgcggcccg gaaagcgata
    60361 acgtgttaaa atccacgcca tcagcagacc gaaaacgccg ttaaaaatcg atgccacaaa
    60421 cgccgacagc agcgtcactt tgtaggccgc gaccacctgc gggttggtga tcacctccca
    60481 gtactgcgcc cagctcatct cagaaagctg catcaccagc gcagagagcg gcagcagcaa
    60541 tatcaggcac acaaacagca gactggtgcc aaggcttaaa gtaaagcccg gcagcacgcg
    60601 tcttgaagaa actgcaaaca ttacttacgc cccgccgcta acagcttgtc taactcgccg
    60661 ccgctggtga agtgggtttt catcacttcc ggccatgagc caaatttgtc ttccacgcgg
    60721 aacagttcgg tctgcgggaa tttatctttc agtttgtcca tgacttccgg gttattcaca
    60781 cggtagtaat agtcggtgat gatggtctgc gcctgtgggc tgtagagcca gttcaggtag
    60841 gcttttgcgg ctttttccgt accgttggcc tgcacatttt tatcgaccca cgccaccggg
    60901 aattccgcca gaatattggt tttcgggatc accacttcaa agccctgtgc ttcatactgt
    60961 ttacggatgt tgttcacttc cgattcaaag ctaatcagca catcgcccaa gccacgctcg
    61021 gcaaaagtgg tggttgcgcc acgaccgccg gtatcgaaca cttcaacgtt tttcaggaac
    61081 tgggtcataa actgttcggt tttggcttta tcgccaccgt cagctttatc cgctgcgccc
    61141 catgccgcca gataggtata acgcgcgtta cccgacgttt tcgggttcgg gaaaatcagc
    61201 ttcacgtcgg agcgcaccag gtcgttccag tcgtggatat tcttcgggtt gcccttacgc
    61261 accaggaagc ccatggtgga gtagaacggc gagctattat tcggcaggcg cgactgccag
    61321 tcagccggga tcagcttgcc tttgtcgtgc aggatttgta cgtcggtcac ctggttataa
    61381 gtgacaacgt ctgcttttaa gccctgcaaa atcgccagcg cctgttttga tgacccggca
    61441 tgagattgtt ttatcgtcag tttgtcgccg ccgttatctt ttgcccattg ctgttcaaac
    61501 ggcggattca gggcggcaaa cagctcgcgg gagacgtcat aagaactgtt cagcagttcc
    61561 gttgcctgta catggcccgc cagcagcaga gaagcgacca gcgcgagtga gttctttttc
    61621 agtaagttaa cggccattgc gcacccttat aaatttaatg actttctaat agccatcata
    61681 tttataacgg acgtgaaagg agtaacggtt ttatataccg tttggtgatt tggaagttaa
    61741 aaagggaata agaacttggt tcaacaggcg taattgttgc aggcagtttg gacacggaca
    61801 gcgcggagaa accggagcgt acacatagta cgtgaggatt tcgagcactg cccggggcca
    61861 aaatgacaaa tgaaatagcc tggtgaactt agttcaagac gaaatcctcc ccacacatgc
    61921 agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg
    61981 ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc
    62041 gccagttcgc cgacttccag cggatcggcg aggcgacaca gcgggattgc ttttgccatt
    62101 tcagtcagca ccgattctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt
    62161 ggcgtgcgga cgtatcccgg gcagatggcg ttaacgcgaa taccggattg cgcgtactcc
    62221 accgccagcg atttagtcag gccaacaatc gccgctttcg ttaaggcata cgccgtttcg
    62281 ccaggatcgg ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg
    62341 cgcgcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgata
    62401 tcaatatgga aatcgcgatc ttcatcgctc atatcgagga aactgcccag acgacaaacg
    62461 cctgcgttat tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct
    62521 gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga
    62581 ccacacagtt cgtccgctag cttttcgatc tcaggggaga tatccagcaa gattaggttc
    62641 gcgccatgac gtgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta
    62701 atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc
    62761 gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca
    62821 ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta
    62881 gctcactgct gcgctgaatc attttcaatg acgccacatc attgatgttg cacaagacca
    62941 acaaacagta aatcggtaac agaattttgc gccgtgcggg tagacatcga cgagctacgc
    63001 cattcggttt caccggaaac ggtatcgagc gtgaaatgcg ccagccgccg cagcggagag
    63061 tcagccagag aagtaatggc aatcaccgtt gccccctgct ttcgtgccgc ttctgcgcac
    63121 aagacaatct cttttttact gccgctgtaa gagatagcga tttgtacatc gccttttttc
    63181 aacgcctggg aaacggtggc ttgtacgtgc gtgtcggctt cgcaggccac gcgatagcca
    63241 attttcatca gtttgaacga cagatcgcgc cccaccagcg ccgatccgcc caggcccgtt
    63301 atctggataa acggtgcttt actgatgacc tcaatgattt tttgcagccg cgcgtagtcg
    63361 aacaacgcac aggtttgctc cagcgccagc tctttttcgc gattcagttt gcgggcaatc
    63421 acttccagcg aatcatcact ggtaatcgaa ctgtgcaggt gcagcgccgt ggcattcgtt
    63481 ttctcccggc tggcactgta ttcgccaatt aacgccatac gcaattcagt aaatccttgc
    63541 gcgccgagct tctgggcaaa tttcacaatg ctcgactggc tgataccgag ctgtttcgcc
    63601 atctgccgtg aagagacgga tttcagctcg ctcactctgg cccgcagaaa gtcagcaatt
    63661 ttttgctcgt tcccggtaaa ttcactttcc gcattgcgta ttttcgtcag gtacaacatc
    63721 ttccactctc ctgggctggc agcaaaaatt ccgcttaagt tcttatgacg ctcttcacac
    63781 tctgcgagtg tagactcaat gattccttta agtctgtcaa ttcgggatat atgattccat
    63841 acatctgcaa ttatttgatc gtaaatagta aggtcaccac cgatgcaact agaaaagatg
    63901 attactgaag gctcgaacac cgcctcggct gaaattgacc gcgtatcgac gctggaaatg
    63961 tgccggatta tcaacgatga agataaaacc gtaccgcctg ccgttgatcg cgtcctgccg
    64021 gatatcgccg cggcgatcga tgttatccac gcccaggtca gcggcggcgg tcgtctgatt
    64081 tatctcggtg cgggaacatc cggtcgtctg gggattctgg atgccagcga atgtccgccc
    64141 acctacggcg tgaaaccggg tctggtggtt ggtttgattg ctggaggcga atatgccatt
    64201 cagcacgcgg tggaaggagc tgaagatagc cgggaaggtg gtgttaacga tctgaaaaat
    64261 attaatttaa cggcacagga tgtggtggtt ggcattgctg ccagcggtcg cacgccgtat
    64321 gtgattgccg gactggaata cgcacgccag ctcggctgcc gcacagtggg aatttcctgt
    64381 aatccgggga gcgccgtttc aaccaccgct gagtttgcca ttacaccgat tgtaggtgcc
    64441 gaagttgtta ccggttcttc gcggatgaaa gcaggtacag cgcagaaact ggtgctcaat
    64501 atgctttcca ccgggctgat gattaaatcc ggcaaagtgt tcggcaacct gatggtcgat
    64561 gtggtcgcca ccaacgaaaa actgcatgtg cgccaggtca atatcgttaa aaacgccacc
    64621 ggatgtaatg ccgaacaagc ggaagcggcg ttaattgcct gcgagcgcaa ctgtaaaacg
    64681 gccattgtga tggtgctgaa aaatctcgat gccgcagaag ctaaaaaacg cctggatcaa
    64741 cacggcggct ttattcgtca ggttttagac aaggaataac ccatggccaa agagatcagc
    64801 agtgaacttc tgaacaccat tcttacccgt gtcggcggac cgggaaatat cgccagttgt
    64861 ggtaactgta tgacgcgcct gcgtctgggt gtacatgaca gttcactggt tgatcccaat
    64921 atcaaaacgc tggaaggcgt gaagggcgtc attttgacca gcgaccaggt gcaggtcgtt
    64981 tttgggcctg gtaaagcgca tcgtgccgca aaagcgatga gcgagctgct gggcgatgca
    65041 ccggtacagg atgccgcaga aatcgccgcc cagaacaaac gtcagttaaa agccaaacaa
    65101 acctccggcg tgcaacaatt tctcgccaaa ttcgccacca tcttcacgcc gctgatcccc
    65161 ggttttattg ccgccggtct gctgctgggg atagcgacgt taattgccac ggtgatgcac
    65221 gttccggcag atgctcaggg cacgctaccc gacgcgctga attttatgaa ggtgttcagc
    65281 aaaggtttgt tcaccttcct agtgattctg gtgggctata acgccgccca ggcatttggc
    65341 ggcacgggcg tcaatggcgc aattattgcc gcactctttt tgctcggtta taaccccgct
    65401 gcaaccaccg gttactacgc cggttttcac gatttctttg gtctgcccat cgatccgcgc
    65461 ggcaatatta tcggcgtgct gattgccgcg tgggcctgcg cccgcattga aggcatggtg
    65521 cgccgcttta tgccggacga tctcgacatg ctgctgacct cgttaatcac cctgctgatc
    65581 accgctacgc ttgcgtacct gatcattatg ccgctgggtg gctggttatt cgaaggtatg
    65641 tcatggctgt ttatgcacct gaacagtaat ccgttcggtt gtgcggtttt agccgggctg
    65701 ttcctgatcg ccgtggtatt tggcgtgcat cagggcttta ttcctgttta cctcgcgtta
    65761 atggacagcc agggattcaa cagcttattt cccatccttt caatggcagg cgcgggccag
    65821 gtgggcgcgg cgctggcact ctactggcgg gcgcaaccgc acagtgcgct acgcagtcag
    65881 gtacgcgggg cgattattcc cggcctgctg ggcgttggtg aaccgctgat ttacggtgtc
    65941 accctgcccc gcatgaagcc gtttgttacc gcctgtttag gcggcgcggc gggcggtttg
    66001 tttatcggat tgatagcctg gtggggtctg ccgatgggct taaacagcgc ctttgggccg
    66061 tctggtctgg tggcgctgcc gctgatgact tccgcacaag gcatccttcc ggcaatggcg
    66121 gtttatgctg gcgggattct ggtggcatgg gtttgcgggt ttattttcac cacgctcttt
    66181 ggctgccgta acgtcaatct ggactgatat tatgaaacgg acaatgctct atctttctct
    66241 gctggctgtt agttgtagcg tcagcgccgc aaaataccct gttctgacag aaggtagttg
    66301 atcaagtata tgcggcgtta aagcagtaat aaaaaggcca gtcagtaatg ttactgcctg
    66361 gcctttaatc aatgaatcag aaacgcttac agcgccatca acttgtccag cgacggtgcg
    66421 aaataatagc cgccggttac cggtttggtg aaacgcaaca tcgcatcacg tttgccatcg
    66481 gtatcgccaa acatgctcag cagttgctgc tcaatgttat gcagacgcgc gcagtaggcg
    66541 cagaagtaca gaccgtgagt gccactggcg gtgccgtacg gcaggctctg gcgaacaatc
    66601 ttcagccctt tgccatcttc tttcagatca acgcgggtga ggtgagaggt ttccggacgt
    66661 tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc cgatcatcat ctcctgatcg
    66721 tgaacgctca tccggttgag ctgcttcagg ttgtgctccc aacgctgcac aaacacatag
    66781 ctgccgccgg aatccacgcc gtctttgata accgccactt cgcggcgcgt ctcttcaccc
    66841 gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac gctcttccac ccaacggaag
    66901 ccgtggatct cttctttcac ttcaatgcag tcaccaaagg cttccatcgc cgcctgagcg
    66961 acagagaagt ttacgtcgtg acgcagagaa agaatgtgga tcaacacatc gaactgggtc
    67021 gtcggcgcaa ggcctttacc gtagcccgga aaatctttca gctcttctgc cccaacgccg
    67081 ccgctcagag cgcgccaggt gttgttacca aaggcaacca ccgcaccaag atgcgcgtcc
    67141 gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg ttttactggc cgcacgcagg
    67201 gcgtcaactt cccctttcac gttggcttcg atccaaatcg ccgcgcggca atgttctggc
    67261 aaaatgccac tctgaacctg agacattgtt cctcctgaaa ataagaatgc cacgcaatcc
    67321 gtggcattgt tgagcgctat tttacgtgtt ttttcgcgac ggcggtttgt tcagacgcaa
    67381 attaacgccg ccagataatt ttactgactt tccagttttt cagggtatcg tccgaaggca
    67441 ttaacccttc aggaccacgc cattccccgc tgaactggta gctaatatgt tgactgcctt
    67501 cggctttaca ttcgacggca cgattatcat caccatcagc tttttggcaa ttgccaaatg
    67561 ctttgctgta aaggtcgcta aacggtgtac cgattttaac accggtgtca gcaggaatat
    67621 cgctatccag cacgtcaatg cgactgatcg tgccttgatc gccgttaatc accatcgcca
    67681 cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt gccgttcgtg gttttcattc
    67741 cgctgcgcag gcgataatcg ccatcaagcg catcagcaat ggcttgttct tgcagtggtg
    67801 tggacgccgt taattcaccc acgccctgct cgctcacttt ggtggacgat ccaaaccagt
    67861 tccacgggtt agcggcagac cagttaactg acgacatcgt ggaacagccg gtcagcatca
    67921 acggcatagc gcataacatt aaacgcagcg atttcatgtc acttcctttg gttattcaat
    67981 aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta ttctttttct tcattaaaac
    68041 aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc cacaatatcc gccactacca
    68101 gcgccagacc gataccgcta acggattcac cgttcagcca cagccacggt tgccagcaaa
    68161 gtaaaaccac ctgcgccaac agcaacagaa aatagagcac atgccaggtg cgaggaaacg
    68221 aagcccgccg accgctcaac aaaaacgcca gcaccgccgg aataccggga atcagcccca
    68281 gccagaaatt atcgtgatcg ggataaaaca gatttagcag cgcagtaccc tgctcacgcg
    68341 acgcaccggc aatgacaaac agcacccagg ttcgcgcctg aagcaatagc acaagccaga
    68401 agagcaaggg taaacgcagg cgtccgtgcg catcataatg gacaggatgg aactcagtac
    68461 tcttcatctt caatcaaacg cttacccaga ctcagcacgt cggcgtgttc atatcccagg
    68521 cgttcataca ttccgagcac catgtcgtta tcttccggca cgttgatctg aattttcggg
    68581 cagccgcgag caatcagctt tttctccagc cggttaagca acgcattggc aattccacgc
    68641 ccacgaaact ctggatgcac gccaagataa taagcagacc cgcgatgccc gtcataaccg
    68701 cccatcaccg ttccgaccac ttcaccgttt acctcagcga ccaaaaacaa actgacgtca
    68761 tggttcatct tacgctcgat gtccatttcc ggatcgttcc acggacgcag caagtcgcaa
    68821 cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc gaaatacgcg tatctccatg
    68881 gtattcgtta cctttttgcg ggttaaaagg ctgattatgg cgtgaacggt cgaattagcc
    68941 aatatctgac gaaaatcggt tgaaaaagtg gcataatggg gagttgtcaa ctattgaaat
    69001 gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg attacgcgat acgatataac
    69061 atctggaact ttattattac aactcaggcc gtatgagcac ttttaaacca ctaaaaacac
    69121 tcacttcgcg ccgccaggtg ctgaaagccg gtttagctgc cctgacgttg tcaggaatgt
    69181 cgcaagccat tgccaaagac gaacctttaa aaaccagcaa cggacacagc aagccgaaag
    69241 ccaaaaaatc tggcggcaaa cgtgtcgttg ttctcgatcc aggccacggc ggaattgata
    69301 ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt ggtgctggcg attgctaaaa
    69361 acgtccgttc cattttgcgt aatcatggga ttgatgcgcg tttaacgcgt tctggcgata
    69421 cgtttatccc actttacgat cgcgttgaaa tcgcccataa acatggcgca gatctgttta
    69481 tgtcaattca tgccgatggc tttaccaacc cgaaagctgc cggtgcttcg gtatttgccc
    69541 tctctaaccg tggggcaagt agcgcaatgg cgaaatacct gtctgaacgc gaaaaccgcg
    69601 ccgatgaagt tgccggtaaa aaggcgactg acaaggatca cctattgcag caagtactgt
    69661 ttgatctggt gcaaaccgat actattaaaa acagcctgac gcttggctcg catattctga
    69721 aaaagattaa gccggtgcat aaactgcaca gccgcaacac cgaacaagcc gcgtttgtgg
    69781 tgttgaaatc gccgtcggtt ccttcggtgc tggtggaaac ctcgtttatc accaacccgg
    69841 aagaagagcg gctgttaggc acggcggcgt ttcgtcagaa aatcgccaca gcgattgctg
    69901 aaggcgtgat cagttatttc cactggttcg acaaccagaa agcacattcg aaaaagcgat
    69961 aagttatgaa acccgacgca caccaggtta aacagtttct gctcaacctt caggatacga
    70021 tttgtcagca gctgaccgcc gtcgatggcg cagaatttgt cgaagatagt tggcagcgcg
    70081 aagctggcgg cggcgggcgc agtcgggtgt tgcgtaatgg tggtgttttc gaacaggcgg
    70141 gcgtcaactt ttctcacgtc cacggtgagg cgatgcctgc ttccgccacc gctcatcgcc
    70201 cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc actggtagtg catccgcata
    70261 acccgtatgt tcccaccagc cacgcgaatg tgcgtttttt tattgccgaa aaaccgggtg
    70321 cagagcccgt ttggtggttt ggcggcggct tcgatttaac ccctttctat ggttttgaag
    70381 aagacgccat tcactggcac cgcaccgccc gtgacctgtg ccagccattt ggtgaagacg
    70441 tttatccccg ttacaaaaag tggtgcgacg attacttcta cctcaaacat cgcaacgaac
    70501 agcgcggcat tggcgggctg ttctttgatg atctgaacac gccagatttc gaccactgtt
    70561 ttgcctttat gcaggcggta ggcaaaggct acaccgacgc ttatttacca attgtagagc
    70621 gacgtaaagc gatggcctac ggcgagcgcg agcgcaattt tcagctctac cgtcgcggtc
    70681 gttatgtcga gttcaatctg gtctgggatc gcggcacgct gtttggcctg caaactggcg
    70741 ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt acgctgggag tatgattatc
    70801 agccaaaaga tggcagccca gaagcggcgt taagtgagtt tattaaggtc agggattggg
    70861 tgtaactccc tcacccccat tcccgcatcc gctgatgcaa cgtcaacgac ggcttctcgg
    70921 caaacagctg ctggtaatcc gtggcaaatt gccccagatg ccagaatccc cactgcatgg
    70981 cggcgtcttt taccgttgtg ctttgcgacc acgggcttat cagttcgcgg cgtacgacgt
    71041 tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc taaaatagcg tgaaacgcgt
    71101 tttgtagcgt gcggcgactg acatgcagtt gattacacaa atccagcacc gtcaccggtt
    71161 cggacatgtt ttccagcaca tattcacggg cgcgggaaag caaccgacgg taactctgat
    71221 gactgatgct ttccgccgtc accattggtt gcgcttcttc cagcatcgcc cccatcgcca
    71281 ttagcaaatt atcccccagc acttttcgca ctgcaggctg atggagattt tccgggttct
    71341 cgcaaaacgt cgccagcgcc tgttggacaa agccccacag cgcggcttta tgctgctctt
    71401 tcacttccag cgccgactgg ctacgcagca tatgtaatac ccgatccgga ttgtgcaaaa
    71461 agttagcctg acaggtgatg acatcttcag acagcactac gcccaggatc gtataatcgt
    71521 ccggcgtact taattcaaac tcggttccac cggggcgcgt cgctatttct gcgcttccca
    71581 gacattgcga accgataaat ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg
    71641 agttcggcca gaccaggcac gactgacgca gcgccagacc ggtgtattca cgaaaaacct
    71701 gaatatcatc gagtagaatt tccgtaaatt caccatgaaa cttgcccgga tgcagctgat
    71761 cgtaaatctg ctgctaggcg gtaatcgtca aagcatgttc atagacatcc gttgtctgtc
    71821 gttgatgaac attatccact tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt
    71881 catgataaag atggtgcaaa ttggctgtac gggtcttttt catgatgtta atgccgggtg
    71941 ttgtaggaca cccggcacct ccgacaggtt aatggggctt gagacgataa cgactactgc
    72001 gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc atttctggct ttttccagcg
    72061 gccagccaaa gtgggcggcc acttctcctg ccgtcattcc ctgacatact gatgttaaca
    72121 gcgccaacaa ctcgtcagca gattccgcga cgataactgg cgcgtcgggt tcctttacgg
    72181 gttgcttcgg ctggcggtta aagccagtaa ccagccactg ggtgtcatcg tctggccgac
    72241 cgatctcctt acggctgatg acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca
    72301 gcgccgcacg acacgccgcc agatcgcctt ccaccaccag cgttaagcga ccagggtcaa
    72361 gcacttcgtg actgagcaga cgaacgttag ctgctttgag catggcatcc gctgcatcta
    72421 tcgcggcgac catgccgtcc acttccagca atcccagtgc attgatcatc ggcaacctcc
    72481 gttacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta aaggcgttac
    72541 aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag
    72601 acggtggcgg gacgtaggtc accagctgta cgtcggcaga tttcaacgcg gcatcaatgc
    72661 cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca agcgtgatcc
    72721 cggcggttga tgaaagataa gaaccggtac gcgaaaccac atgcgccagg aaggcggtgt
    72781 tttctgcgtc attggcccac tggaaagctg cgccattttc aatatgtgca accatcgcat
    72841 ccagaccggc acgcacttcc gccgggtttg gcccaccaag cataatcagc acttcaccgg
    72901 cagtcggtga cgggccgtgt gccgctcccg catacagcga gcgaccatat accacttcga
    72961 ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt tacgtcgtca gaatcggcag
    73021 aaatcagccc gaggctacga atatgcggcg gcaattttag ttcacgcgca aattcagcgt
    73081 taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct aaagctggca
    73141 tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc atacgtttcg
    73201 ccaaatccac aatgacggca cagcagcacg aaagagaagc aaaagtcagc tacctggtac
    73261 actaggcagc gggtcagcaa aggggggcaa atgccccttt tttcatgtca gataatcaga
    73321 tcattacctc aataaactaa taatcacatc gcggttacaa ccagtttgat agtgatagct
    73381 caaacccaga cgtggcgctg gctgcaagca aaataagccg caaaaatgcg accccatgtt
    73441 tactagccgc aaaaatgcgg tcctatttgc cagaaccttt tattattcaa ttggttagtg
    73501 caagaaaaag cgtgattact gttcaagaag tatccagaaa ggtcatgatt ttgacgctga
    73561 aaaaaaatag gtgggcattt ttgcccactc actagccgca aatttgccca ctcactagcc
    73621 gcatttttgc ggcccaaagt aataaataca gagaaataat aaatacagat cagcgccaag
    73681 atttttcgtc ttttcggcgg aatcatgaga ctagtgctta cgtcctgata acctccagag
    73741 cagaccttaa cttctgtgca gcaggactac gctcattgcc gctcggctag gtcaaatcct
    73801 cacgataaag ctgttcggat tgtgaccata atgttaaagg actctttaaa tcattccttg
    73861 acgaattaac tagctggctt aagcggaata gcatgcagga taaaaacaaa accagcttag
    73921 cagtggggag agcttcgctt cgctcgtctc ctggggccaa tgctcaatga aacgacgtaa
    73981 ggcacggagc gctcaccata ggtagacaca taaacatctg agagcgcacc ttctatcgcg
    74041 ctacgcgctc aataaaaacc aacgacaccg ccacactgca agaccaatgg tttttatcgc
    74101 gtatacagac cagacaggaa acagcagcaa agcagcgtat agccaggtgc agacagcaca
    74161 ctcacacgac gcacagcagg cttaagtgat cactcaggaa actttatccc cgccgccgat
    74221 caccgcgaac tatcatgacc gcgccaaatt cgaaaggaat ggcccataga acgcgatgaa
    74281 cacacataag cgctacactt acttaacgtc gccacagagc gattgagagc gcttaaaatc
    74341 gatgcgattt cattaaattt ttctcactcg cctgaatggt ggcgatcagg actgatcgca
    74401 acattgcccc gcctcgatat tgagcgcata atagatacat acattaagcg gaaaagagat
    74461 aagtttattg tgaaaatcag ggggttacat atcatgcgca tatcagaaca acaacggcag
    74521 atcctcgact atctggcaaa aaacgaatca atcaccgcac gacaagccgc agatctcgtt
    74581 tatggtggca atgttacccg cgtccaggtt gacgccgccc gccgctcact catgacgatg
    74641 gttagtaatg ggctaatcag gaaacaaggc cgcgctttcg tagctgaaag acatatcaat
    74701 cacgcttata gccaggacgt tgaagaattg cgccgccaga tcctttgggt attcagcaaa
    74761 cagggtcagg agtatttcct ttctgaacac ccaaatttcg ccgccaaaga gttaacagta
    74821 agcggattgt cgtgcgcact ggattatctt gtcggtgaag gttgcgttac ttacaagcag
    74881 ctatggcacg ccctgaatac gcttgtagaa gatggtttac tcattgccac gcgtgagaac
    74941 atcgaatact ggataatgcc gcgttcaggt cgccgcaatc atgcacggta tatttcaggc
    75001 aacagataca gaacgccgga ctttaatccg acacaggaag atcgggaccg tgccgaaatg
    75061 atgtgcaggg aacaggagaa aagaattatg gataagttat tttcgatgca gtgaacacaa
    75121 gcgggcgcaa tgcccgtttt tattgcctta ttccacgcct gacgaatcaa tttgatcaca
    75181 cctgagaaaa tgcatagcca gaagttaagg ccgttatatt gcgattggga gcgttttaaa
    75241 cggtatcaat ttaattaatt ctgatgtggt gatttgcagg caaaaaaaac ccgccggaga
    75301 agcgggaaat agagacatat cgttgtagtt attaacagat cactttgagg tcgagggcgg
    75361 ctaaccctcg ctaatattat cccgctatta ccttgcgggt gcaaataatc ttttattgct
    75421 ttgatgattt aataaatagc actttttgct aacaaaaacc gctgtcaata tatggccaaa
    75481 aatgtcaaca gaaaaaataa ggtttataat attaagtatc acatgggtaa tgggttcagg
    75541 gaatcggaat ccaccgcctc acggaaattc aaaatttgct tcttctttcc tccgtgggcg
    75601 gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt tcgcagcaat
    75661 ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa ccggagtccc
    75721 tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat gcaaagaatg
    75781 gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac atcatgacca
    75841 atatcgaaag attagaacgc tgtgccgata ttatgcgacg cagatggatt tatgaccctg
    75901 aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc atgaccaata
    75961 aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc acctatcaga
    76021 aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa gtgcaccacg
    76081 ttaatttgaa caaacaggat aaccgcatca agaatattcg cctgatgccg aaagaaaagc
    76141 accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca caatggcttc
    76201 aaccgcaatt aattgaacaa tcactataaa cacaggagta aataccatgc attcattaga
    76261 cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc gtgaggaatt
    76321 ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg aaacggttcg
    76381 tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag atatggaagg
    76441 cactgcaaag cggcgactca tttgcgaatt aagtcgtcgt tttggcgtgt caataagcgc
    76501 ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta ctggcaaata
    76561 aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa cgcgcactgc
    76621 taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt aatgaagtta
    76681 tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa gccgccggac
    76741 tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg tacaaacaga
    76801 gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg gcgcaggttg
    76861 aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat ggagtggata
    76921 gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat tttgatgccg
    76981 ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct gagcaacgga
    77041 aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag ccgggaatta
    77101 ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac aggataagca
    77161 gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc atcatgttgg
    77221 cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta gacctgaaac
    77281 attcgccact gagcaaacag gccatgaagc gccaaacgct tcgtctgtgg gctgaatatt
    77341 cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac cagagcgcgg
    77401 aggaaatgga gttcatcagg cgtttaattc tcattcgcag agacattcat agccagttac
    77461 acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac aagtactatg
    77521 cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga tactcgaagg
    77581 ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca ggaacaacga
    77641 agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg agtgttacaa
    77701 ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag aataacacca
    77761 cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat ttagtgtgcc
    77821 acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc tctcacatat
    77881 gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata aagtaatttt
    77941 ttgatatgtt tatggtttta tattaattaa aatatatcta aatttgatag ttttaaaatt
    78001 gcaaaatcca gattgtgttt gtaatgttta atctgattgt attgagtggc gtttattatg
    78061 aaaaataaat caggtaattc ttgttataga gaagcagtcg atattcatag ctcacttgag
    78121 ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc tcgaaatgta
    78181 ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa cttagctact
    78241 ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc tattgataaa
    78301 aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg ctgtgtgcgt
    78361 atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg ctcaaccatt
    78421 ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg gtgtaaaaaa
    78481 cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa tatgctggca
    78541 taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg atcatcacct
    78601 gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg gtgtcgttac
    78661 gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta agataagtgt
    78721 taaagttaat attagttttt atatttacat cgtaattata tgtttctatt ttaaaaatgt
    78781 tcgagagtat aaaaatgtct aatgctaata tcgcacccaa aagtaagaaa atgacagtgg
    78841 ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata ccttctgaag
    78901 cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg tttttagtac
    78961 ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg ggggcatttc
    79021 gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt atgacctggc
    79081 tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctcg gttgcattta
    79141 ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat attttatttt
    79201 tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt ggtattttct
    79261 cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt atccttgtta
    79321 ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca ttaggttggg
    79381 gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc agcatatttc
    79441 ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat aatccaacta
    79501 aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata ttcctgctca
    79561 gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact caaagtttgt
    79621 tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc tctgtgatgg
    79681 catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca gggcctaata
    79741 caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa aaaaccaatc
    79801 gtcatggaat ggggcaccat ctaatgtttg tacaaggtat aattgtctca gttctttctg
    79861 ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca cagttaacag
    79921 ttatgctcta tttagttatg tatatgctca tgttcgctag tgctatctat ctacgacatt
    79981 ctcaacctga tacaaaacgc ccatatcgag cccctgcttt atttttctgg gcaacacttg
    80041 gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag caaatcccaa
    80101 ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta tttttattaa
    80161 ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac tctgatttcg
    80221 aaccattttc ttgggaaaaa agaactagta attaaatttt ccataagcta caaaagatga
    80281 ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt gtcggtggat
    80341 tgacatcggt aaaggaaata attaatttgc ttcctggtga agatattatc tattgtggag
    80401 ataatatgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt ttgactaaaa
    80461 aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgtcgca tgtaatacta
    80521 tatcctcaac tttggaatcc gaagaataca gcggatatgc taagagcttt ccgtttccta
    80581 tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcaatat aaaaatgttg
    80641 gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta ataaaaaaat
    80701 tgaattcaac aataaatgta tttggagaac caagcaaaaa tcttgcaatg ttaattgagg
    80761 aaggtaatct taatgctcct gcaatcttga atgacatcaa aaaacatgtt aaccatttaa
    80821 tttccctcca tccagtaaat gaaattattc tcggctgtac tcactatcca attgttcaga
    80881 atttttttga agcagttgca ccggatataa aatttattaa tcctgcacat gatcaggcta
    80941 tctctattaa aaatcacttg ggacagctaa atttattaaa ctcctcaaat ataggaactt
    81001 tgcaaataaa tactagtgga agtatggaga tctacaaaac ggtactaggg gaattgagca
    81061 ttacgaaacc tcatacattc agtatcaggc agttttaaat ttatccggta caccgtaaaa
    81121 tcccgtcctt ctgaactgca ccccaaaagt tggacaccaa ctgagtaagg tgcattttat
    81181 ggacaaaccc agttacatac tgggtagtgc atccaattag tagaacatgt gtttttcgat
    81241 aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga ttgttttacg
    81301 acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc gttgggtgaa
    81361 tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc cccagtcatc
    81421 gctggtcagc atgccgatgt tgaagggtgt aagcagtgcc agtagctccc ggcacgtttg
    81481 atcggttcgg ggaccaaaag tgtaggccag tacaccgcct gttttggtgt tgtacgcgta
    81541 cccgagccaa tgttgccggg ctttactgcc aacgtagctc cattgctcat caagctcgca
    81601 gataaacgcc acatcagcat gggcaacagg cgaagaagtt attcgctttg gcgcgagttt
    81661 tttaaagtcc ggatgacggt gttactacca attttcagtg tcctggcggt atcgcgaacc
    81721 ccggcaccat taaaggccat ttcagttatc agctctttca tacccggctt acgtgcttga
    81781 taagtataag tgaactgaaa cacacggtgg cagtcacggc agcgaagtct gtcacggcct
    81841 ttagggttct gaccatggcg gtaaacctga gctgactgac aacggggaca atgaatatta
    81901 acgctggtca tgagataacc tcaaaagccc gtattataca tcagattcaa ctaattagag
    81961 gcatcaccca tccagcatta taaaacatac taacaataac taaattataa taatcacaac
    82021 ttacaatcaa ataataaatg cataagatta acaatgattt tttcgggtta tatttatcta
    82081 aaaaagcaga acattatgta acttattatt ttctaagcga aaatttattg tgcggtcaga
    82141 ctaacaaact tgcgtaccac ccgaagaaca tgcgaagatt taagtattct ttcttttttg
    82201 gtttttattt ttcattttct cttttcttct ctttccggtt ctgttctggt tttggttttt
    82261 tcctttttat ttttattctt tcttcagggt tatgctaaaa caccaaaaca tttttcacca
    82321 caaaagcatc caatcatctt gccagaccaa tcaccttaac cggatacact gccggatata
    82381 atcccactaa cacccagaca ccaagcacca tacctgataa ctaccgtatt caggaaggaa
    82441 atgcgagtga tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt
    82501 cgatatagtc ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg agttgttgta
    82561 gctgtgggcg caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca
    82621 ggggatgcgg attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg accgtcagaa
    82681 ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacacg tcagttttgg
    82741 gatcaagatc cagctgaagc agcgcctgtt gccaggcact caccagatcc gtttcatcaa
    82801 tcgggatcgc caccggcaaa ttgcgcagcg gcagttgtac gccctccagc cagattgtgc
    82861 tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggagcgt acggtttgcg
    82921 ccggaaactg cacattcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa
    82981 gtgggccaat atcggcaaaa cagaacgggt tggcgggctg gtggcgataa cattcgccca
    83041 cgcctccaga aagcgtaatg atttcgggcg taacacctgc gggcagcaaa ccggtttgca
    83101 tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agcgccgcca
    83161 tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcgtca gtgcctgcac
    83221 cgaagcactc atccacaatc atctgccccg gtttatgagc gtaaaccacg cgcccctggc
    83281 tgtcggtttc cagcaggcga ccaccgacgt tgagacaggc agtgccgctg atttttccgg
    83341 catcgaacag ggcgtagttc gcggtgccac cgccgatatc gatattcagt acccgacaca
    83401 gccgttgttc agaaagggtt tgcgccccgg ctccgtgacc ggcgatcacg gattcgaggt
    83461 gcggcccggc gctggcgacg acaaaatcgc ccagcgactg tttttccttc atcacttttt
    83521 ccatcagctg tgcgaccagg ctttcggtaa actggccttc cggcagctgg gcaatgatgg
    83581 tttcgcggat gcgctggctt tcggttttgt cggctggcac gctggcgggc acggacgccg
    83641 ttaccgggat cgattcatca cattcggtga tggtaaaacc cagtagatcc gccacttcac
    83701 gggcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg
    83761 cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg
    83821 gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc
    83881 tcgaaaatcg gctcttcgaa acccgtctca agcaataact ttcgcgttgc ggcgacgtca
    83941 gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac gccaatatcc
    84001 agcaacccgg caggcaaccg actttccggg tcatttgcgc catggacata gatcagcatg
    84061 tctacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggct aaaatattca
    84121 cccggcgtat caatatcgcc cttatcatta aattccacgg ccagtgtttt tctggcgagg
    84181 gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac gctacccaca
    84241 aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca aagcgtataa
    84301 tttaataagc gacctaaacc gctgacggtt tgtgataacg cttcttctac tgcgcccacc
    84361 gagccataaa tcaccaatgc accgctgaag cggtcgagaa aaccgatatg cacatcagcg
    84421 gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag cgtcataatg
    84481 ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg
    84541 atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta
    84601 tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa
    84661 ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcaataagaa tggagagata
    84721 tttactgtta tgaataaact ttcatatgcg acgcatgaat ataaataaaa taatatcgag
    84781 atattttata tactgatttt tttattattc gcaaactcag taataagctt tgctaatata
    84841 tttcaggaaa accgcttacc ctgaatattc agggtaagcg tgagagttaa gaatgaatta
    84901 cagcggttgg gtttgcgctt caaccacggc cagcgccacc atattgacga tacgacgcac
    84961 cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca ccggaccgac
    85021 agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg cagcttccat
    85081 gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca tacggtcgtt
    85141 gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca tcagatctgg
    85201 cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga
    85261 agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa aacgacggac
    85321 agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt cgtcattgac
    85381 gtaggtatcg gcaataaagg tgttaccact cggcagcagc agcgcattca ttgcacctgc
    85441 ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acactaaaat gttcatgata
    85501 atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca tgatcgcgcc
    85561 gatcactgtc gggttactga tcagcgcacg ctgcgcctgt tcctgagtga caccgcgacg
    85621 cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg attcgttatt
    85681 gacgatttca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc
    85741 gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta ccagttcctg
    85801 agtcgcatgc agaacgcgcg cctcttcccc ttccggcaga actacgcgct tcggcgcttt
    85861 gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga actcagtcag
    85921 cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg actccatcgc
    85981 cgcttttgcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg gttttggaat
    86041 gatgtattcc ggaccaaagc tcagatcctg aacgccatac gctgaagcca ccacttcact
    86101 ctgttctgca tgggccagtt ctgcaatcgc gcgtaccgcc gccagtttca tctcttcgtt
    86161 gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg aacgggaagc aaagaacgtt
    86221 gttcacctgg ttcgggtagt cggaacggcc ggtacagatg atggcatccg gacgcacttc
    86281 tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga tcattggtgc
    86341 ccgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac agcccaggaa
    86401 aatatccgca ccttcaatca catcatcgag ggtacgtttg ccgtcatcca ccaccgcata
    86461 tgcggctttg gtttccgcca tatttggctc acggccctga tagataacgc cttttgaatc
    86521 gcaaaccacg atgttatgtt tttgcagccc cagcgctacc agcaggttca tacaggcgat
    86581 tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt tctccaccac
    86641 gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct gatcgtcgtg
    86701 gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac attctggcgc
    86761 tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg agcgcggcga cgacttcaat
    86821 aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc cagcgaattt
    86881 cttaaataga acgcccttgc cttccatcac cggtttgcct gccagcgcgc caatgttgcc
    86941 taaccccagc accgccgtac cgttagagat caccgcaacc aggttaccgc gcgcggtata
    87001 tttgtaggct tttagcgggt ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga
    87061 gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa cctggatttt
    87121 ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt catccatttg
    87181 gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa
    87241 cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc aaactctcac
    87301 cattaatata atgttttggt aataatccta taacactgat gttacctgct taatccagca
    87361 ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc agaagtgtga
    87421 attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa cgagttagac
    87481 ggcatcaaac agttcaccac tgtcgtggca gacagctgcg atattgagtc cattcgccat
    87541 tatcatcccc aggatgccac cactaatcct tcgctgttac tcaaggctgc cggattatca
    87601 caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg caagacccag
    87661 gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca atttcggtgc tgaaattctc
    87721 aaaatcgtac ccggttgcgt gtcaacagaa gttgatgcac gcctctcttt tgataaagaa
    87781 aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg cgttgagaaa
    87841 tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa ttcgcgcggc cgaagagctg
    87901 gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttaccca ggcgcgggcc
    87961 tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag ggcgtattta tgactggtat
    88021 caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt taaatcggtg
    88081 cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat gggcgcgagc
    88141 ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac tatcgcaccg
    88201 aatttgctga aggagctgca ggaaaaagtt tctccagtgg tacgtaaatt aatcccgcct
    88261 tctcagacgt tcccacgccc agcacccatg agcgaagcgg agttccgttg ggagcacaat
    88321 caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc tgttgatcaa
    88381 cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt gttatatgtc
    88441 ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgctgtac aaaaagccaa
    88501 ttctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc tgtggaacga
    88561 ttttcttaaa cataatccta ccgacccaac ctggtatgat cgcgaccgct ttattctttc
    88621 caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt acgacctgcc
    88681 gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc acccggagat
    88741 tggctatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt tggcgaacgc
    88801 cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc cagaccatga
    88861 gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg aaggtatttc
    88921 ccacgaagtc tgttcgctgg caggcacgct gggactaggc aagctgattg gtttttacga
    88981 tcacaacggt atttccatcg acggtgaaac agaaggctgg tttaccgacg atacggcaaa
    89041 acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc
    89101 ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc tgattatctg
    89161 ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc
    89221 accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc accatccgcc
    89281 atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag gcgaaaaagc
    89341 gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc aactggcaga
    89401 agagtttccc cgacggatga gcggtggttt accgaaggac tgggagaaaa cgactcagaa
    89461 atatatcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg cttcgcaaaa
    89521 tacgcttaac gcttacgggc cgatgctgcc tgagttgctc ggcggttcgg cggatctggc
    89581 tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc cggcgggcaa
    89641 ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg gcatcgcgca
    89701 ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat acgcccgtaa
    89761 cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata cccacgactc
    89821 aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg ccagcctgcg
    89881 cttaacgcca aatttcagca cctggcgacc gtgcgatcag atggaagcgg cggtgggctg
    89941 gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa ggcagaatct
    90001 ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg gttatgtgct
    90061 gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag agatggaaat
    90121 taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg tagtttccct
    90181 gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg tgttgccttc
    90241 taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt acaagtatgt
    90301 tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc cggcggataa
    90361 gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct
    90421 gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta tgttcccctc accctaaccc
    90481 tctccccaga agggcgaggg gacggtccgt gcgcttttta gggccgcatc tgcgattttg
    90541 tttaacgggt gatccacaac gtgggcctgg cgtctggccc atgccagtta tcgcaggtag
    90601 gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa
    90661 tgccacaatc gcttaatccg cgccctttcg ctaaggtcac cagttcacgc gatttctcat
    90721 caaatgttgc gttcatcagt tccagttcat tcgtctcctg gccgttgttg aacggcaaac
    90781 gcaaccgaac cgggcgcgag gctagcggct ttttacgcga cacaatccat gccaaatcaa
    90841 tggtgttata ggcccctgcc tcacagctaa tcatcatcag tgctttatca tcggtcagcg
    90901 cagtgacatt cacctcacga cgtaacggat caagcgagca gcgcagacca ttcatccgcc
    90961 agtttccata atccagcaaa tcgttacgtt cttcgagtga gagtggcgtc ggcgttgggt
    91021 taaccaccgc gacctctttc agcgcaggcg cgggcggtac gctgagaggc ggttcgtccc
    91081 ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca ataaacaaca
    91141 acgccgcttt taagccgctc agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct
    91201 tcccttcctg aattatctgc aaaaacgcgg tgatggttgc cgtatcatcg gttactaata
    91261 accatggtga aatccgccac ttgtcaccac ttaatgccaa cggctcacca tctaatagca
    91321 gccgtggcgc tatctcccct tcagacgcat ccggcgactt caacccaccg cgctcaatac
    91381 gtaaaacggc atcggtatgc gccccagcgc tgcggctcag ggtcatcacc agtccattat
    91441 gatcgcccgt attgcgcgcc acacaaaaat tttgattatt acaggtgacc tgccagtcgg
    91501 aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa
    91561 agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa acaaacagca
    91621 caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg gatttgtcgg attgagccaa
    91681 taataaacaa cgaaatatta ttgctatttt tcagtccatt aaatgtgaca tttgcaaata
    91741 gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca tctccagcgc
    91801 ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc caccggcgtt
    91861 agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag tcacaccgcc
    91921 aggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc ccgtctcttc
    91981 aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc cggcgcaggt
    92041 ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga actgacgaat
    92101 cagaaccacg ctctttttct tcgcgttgta caggaggatc gtcgcaccat taccgcgatc
    92161 gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat cgtaagtaat
    92221 gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg tgatttgttg
    92281 cgtcataccg actccacagc gcgaaatgaa caattatatt acgctgtgaa gtcgggtttg
    92341 tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtca ataatcccct
    92401 gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca cgcacggcgt
    92461 cgccaccggc gaagattttc ggattggtgg tctggtaacg gtactggctt tccacatccg
    92521 cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac ggcatcccgt
    92581 gtggattgaa gccaaacgcc ataatcaccg catcggctgg catgacaaat tcactgccct
    92641 caatcggtac aggtcgccga cgcccctggg catccggctc gccaagacgc gtgcgcagga
    92701 aacgaattcc gcaaacgtga ccttgctcat tcagctcaag cgccaccggc tggacgttaa
    92761 attcgaagtt ggccccctct tcgcgggcgt tcttcacctc tttcttcgag cctggcatgt
    92821 tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac
    92881 gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt ccggcggtat
    92941 tgataaacgg ctcttccggt agctcttcga gccccatcac ctgtttagtg ttggcaatga
    93001 ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc gctttcatgg
    93061 aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc aaatccaaag
    93121 agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg ctgaagattt
    93181 cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg aaagtgagca
    93241 agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa
    93301 catccgcaca ggccagccct gccggacctg ccccgataat cgccacccgc ttgtccactt
    93361 tggtgacatg acttaagtca ggacgccaac ctttcgccaa cgcctgatct gaaatatagc
    93421 gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg aatagtacag gcaccttcac
    93481 acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg gtctggtgag
    93541 aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac tgcggaatat
    93601 ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgaactta aggcagcgcg
    93661 aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca aaaccggttt
    93721 tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt
    93781 cgactttact cattaccggc atttcttgtg cagcggtact ggcatgccac ggttgatgct
    93841 cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg acgtcagtga
    93901 ccagttgcag cgcgtccgcc gggcaattct cgacacaggc aggaccgttt tcgcgccctg
    93961 cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg ggtgtcagga
    94021 cgatttgcat cgtaccaaac ggacaggcca ccacgcagga tttacagcca atacactttt
    94081 gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg cagctacggg
    94141 cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgc tgatgtttga
    94201 taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca tcattgtgag
    94261 ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg ttggccataa
    94321 taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt tatatgagtg
    94381 ttgttattac ccgacttaca ggggaaccgg caatgttcat gtgcccagaa taagtaacta
    94441 tttcgctata aactgtggca gatcaaataa tcccatcact gactaaattg cgtttcagcg
    94501 aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa cgacccgtct
    94561 cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt tccacgggta
    94621 tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat attgccggat
    94681 cgctgcgtat gcagagttac cgcctgggct acgacttgca aagtggcagt ccacaactca
    94741 atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc aacctcaacg
    94801 tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc aactggctgg
    94861 agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat attaataatt
    94921 atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc
    94981 tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg gtctttttta
    95041 ctttgcgccg catacgccat caggtggttg ccccgctgaa tcagctggtt accgccagtc
    95101 agcgtattga acacgggcag ttcgactcgc cgccgctgga taccagtctg ccgaatgagc
    95161 ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga gctgcataaa ttgtaccttt
    95221 cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgagaccaag cgtcgtctgg
    95281 aggtgttgta tcagtgcagt gttcccaggc gctgaacacc agccagattg atgtgcattg
    95341 tttccgccat attttgcaga ttgttcgcga caatgaagca gctgaatatc tggagctgaa
    95401 cgttggggat aactggcgga ttagcgaagg gaagccaaac cctgagctgc caatgcagat
    95461 tttaccggtg acaatgcaag agacggttta cggcgaactg cactggcaaa atagtcacgt
    95521 ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg ctgggacgcg gtttgtactt
    95581 taatcaggcg cagaagcatt ttcagcagtt attgttgatg gaagaacgtg cgaccatcgc
    95641 ccgcgaattg cacgactcgc tggctcaggt gctttcttac ttacgtatcc agttgacgtt
    95701 actgaagcgt tcgataccgg aagataacgc caccgcacaa agtatcatgg ccgatttttc
    95761 ccaggcgttg aatgatgctt atcggcagtt acgcgagctg ttgactactt tccgcctgac
    95821 gctgcagcag gcggatctcc cctccgcgtt gagggaaatg ctggatacgt tacaaaatca
    95881 aaccagcgct aaactgaccc tcgactgccg tctgccaacc ctggcactgg atgcgcaaat
    95941 gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg aatgcgatga agcacgccaa
    96001 cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac ggcaatcaca cggtttatat
    96061 ccgtgataac gggattggta tcggtgaacc gaaagaaccc gaaggtcatt atggactgaa
    96121 tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg actttttcac aaccttccgg
    96181 cggcggcacg ttagtgagta ttagctttcg ctccgcagag ggtgagggga aggggaacac
    96241 gccggctgag gtgtaaataa ttaccctcac accggccctc tccctttaaa ggcgagaggg
    96301 aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc aatctcatga taatttacat
    96361 taactccttt ttttctccac gattggctcg taccttgccg ctacagtgaa gcaagtcaag
    96421 cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg gcgaatttct ttattgatcg
    96481 ccccattttt gcctgggtgc tgacaatcct gttgtgtctg acaggtaccc tggcgatttt
    96541 ttcattgccc gttgaacaat accccgatct cgcgccaccg aatgtgcgag tgaccgctaa
    96601 ctatcccggc gcatcggccc agacgctgga aaacaccgtg acccaggtta tcgagcaaaa
    96661 tatgaccggc ctcgataatc tgatgtatat gtcatctcag agcagtggca ccggtcaggc
    96721 atctgtcact ttaagtttta aagcaggcac cgatccggac gaagccgtgc agcaagtaca
    96781 aaaccagctg caatcagcca tgcgaaagtt accgcaggcg gtgcaaaatc agggcgtgac
    96841 ggtgcgtaaa accggcgata ccaacattct gaccattgcc ttcgtctcta ccgatggctc
    96901 gatggataaa caggatattg ctgattatgt tgccagtaat attcaggacc cgttaagccg
    96961 cgtgaatggc gtcggggata tcgatgccta tggttcgcaa tattccatgc gtatctggct
    97021 ggacccggcg aaactcaaca gtttccagat gacggctaaa gatgtcactg atgccattga
    97081 gtcacagaac gcgcagattg cggttgggca acttggtggt acaccttccg tcgataagca
    97141 ggcgctcaac gccaccatta acgcccagtc actgctgcaa acaccagaac agttccgcga
    97201 tatcaccttg cgggtgaatc aggacggctc agaggtaagg ctgggcgatg tcgccaccgt
    97261 cgaaatgggg gcggagaaat acgattatct tagccgcttc aatggtaagc cagcctccgg
    97321 gttgggggta aaactggcct ccagcgcaaa cgaaatggcg acagcggagc tggtgctcaa
    97381 tcgtctcgac gagctggcgc agtatttccc gcacggactg gaatacaagg tggcgtatga
    97441 aaccacctcg tttgttaaag cctccattga agacgtggtg aaaacgctgc tggaagctat
    97501 cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca cgctgatacc
    97561 cactatcgcc gtgccggtgg tgttgatggg aaccttctcc gtactttacg ccttcggtta
    97621 cagcgtcaac accttaacca tgttcgcgat ggtgctggcg atcggtctgc tggtggatga
    97681 cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt gaggaaggac tcactcctcg
    97741 cgaagccaca cgtaaatcga tggggcagat ccagggggca ctggtcggga ttgcgatggt
    97801 gctttcggcg gtatttgtac caatggcctt cttcggcggc accaccggtg ccatctatcg
    97861 ccagttctct attaccattg ttgcggcgat ggtgctgtca gtactggtag cgatgctcct
    97921 cactccggct ctgtgtgcca cgctacttaa gccactgaaa aaaggtgagc atcacgggca
    97981 aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc aacgccgaac gctacgaaaa
    98041 aggggtggcg aaaattctcc accgtagcct gcgctggatt gtgatttatg tcctgctgct
    98101 tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc tggaagaccg
    98161 gggcatgttt actacctcgg tacaattgcc cagcggttca acccaacaac agaccctgaa
    98221 agtcgttgag caaatcgaga aatattactt cacccatgaa aaagacaaca tcatgtcggt
    98281 gtttgccacc gttggttctg gccctggggg taacgggcaa aacgtggcga gaatgtttat
    98341 ccgcctgaaa gactggagcg aacgcgacag taagaccggc acctcgtttg ccattatcga
    98401 gcgtgcaacg aaggcgttta accaaattaa agaagctcgc gttatcgcca gcagcccacc
    98461 agcaattagc ggtcttggta gttctgcagg ttttgatatg gagttgcagg accacgctgg
    98521 agcgggtcac gatgcgctga tggcagcacg taatcagttg ctggcgctgg cggcggaaaa
    98581 cccggagcta acccgtgtgc gccataacgg cctcgatgac agtccgcagt tgcagattga
    98641 tatcgaccag cgtaaagctc aggcgctggg cgttgatatc gacgatatta acgacacact
    98701 gcaaaccgcc tggggttcga gctatgtgaa tgactttatg gatcgcggtc gcgtgaagaa
    98761 agtctatgtg caggcagctg cgccgtatcg catgctgcca gatgacatca atctctggta
    98821 tgtccgaaat aaagatggcg gcatggtgcc cttctctgct ttcgcgacct cacgctggga
    98881 aacaggctcg ccgcgtctgg aacgctataa cggttattct gcggttgaga ttgttgggga
    98941 agccgcaccg ggggtcagta ccggtacggc gatggatatt atggaatcgt tagtgaagca
    99001 gctgccaaac ggctttggtc tggagtggac ggcgatgtcg tatcaggagc ggctttccgg
    99061 cgcgcaggct ccggcgctgt acgccatttc cttgctggtg gtattcctgt gtctggcggc
    99121 attgtatgaa agctggtcgg taccgttctc ggtaatgctg gtcgtgccgc tgggggtaat
    99181 cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac gacgtttact tccaggtggg
    99241 cctgttaacg gtcattggtt tatcggcgaa aaacgccatc ctgatcgtcg agtttgctaa
    99301 cgagatgaac caaaaaggcc atgacctgtt tgaagcgacg ctccacgcct gccgtcagcg
    99361 tttacgcccg attctgatga cctcgctggc atttatcttc ggcgtattgc caatggcaac
    99421 cagcacgggt gccggttccg gtggtcagca tgcggtgggt actggcgtaa tgggcgggat
    99481 gatttcagct actattctgg ctatttactt cgtgccgctg ttctttgtgc tggtgcgccg
    99541 ccgcttcccg ctgaagccgc gcccggaata agcaataaaa aaggcgacat gccaatgtgt
    99601 cgcctttttc aactttccga ttaagaacct gctcagcggg ttcttgctgt ttatactttg
    99661 tctcaggaat tacttacgaa gcataacttc gataaagtct ttccagttcc ccagttcacg
    99721 ttcaatcata acaacctctc ttataattat gggtattcta cggaaacaat ataccgtggt
    99781 gaagctaatt tactcgattg ctgcgatgac tacctccggg ggacaaacct tatgtaaata
    99841 ctatggtcct gcggtgataa tttgtatgtg atacacagca acatttcgag atattcatac
    99901 ggcatctaat actgatttaa ttctggttaa aatacagaca gataacaaga tgaatattct
    99961 taatgtttac gttaaaaatg tttaatatta tttaatagtt gttaatttga atacttcgat
   100021 aatgttatat tttctgataa tcatttgcag gcaaaatgtt ttcaccctta aatgagtatt
   100081 tattctcata aatcgaaaaa ggattcatta tggttacact ttacggcatc aaaaattgtg
   100141 acaccattaa aaaggctcgc cgttggctgg aagccaataa catcgactat cgttttcatg
   100201 attaccgcgt cgatgggctg gacagcgaat tattgaacgg ttttatcaac gaattaggct
   100261 gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa actggacgaa actacccgca
   100321 ataaaatcac cgatgcggcc tctgcggcgg cattaatgac tgaaatgcct gcaattatca
   100381 aacgtccatt gctctgcgcg cctggtaagc ctatgctgct gggtttcagt gattccagtt
   100441 atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc ggttattgag ctgacacaac
   100501 agcttattcg ccgcccttcc ctgagtcctg atgacgcagg atgtcaggct ttgttgattg
   100561 aacgtttgca ggcgatcggt tttaccgttg aacgcatgga ttttgccgat acgcagaatt
   100621 tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc cgggcatacc gacgtggtgc
   100681 cgcctggcga cgccgatcgt tggatcaatc cgccgtttga acccaccatt cgtgacggca
   100741 tgttattcgg gcgcggtgcg gcagatatga aaggctcgct ggcggcgatg gtggtagcag
   100801 cagaacgttt tgtcgcacaa catcccaacc atacggggcg actggcattt ctgatcactt
   100861 ctgatgaaga agccagtgcc cacaacggta cggtaaaagt cgtcgaggcg ttaatggcac
   100921 gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc gagtatcgaa gtggtaggtg
   100981 atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa cctcaccatt catggcgttc
   101041 aggggcatgt tgcctaccca catctggctg acaatccggt acatcgcgca gcacctttcc
   101101 ttaatgaatt agtggctatt gagtgggatc agggcaatga attcttcccg gcgaccagta
   101161 tgcagattgc caatattcag gcgggaacgg gcagtaacaa tgttattccg ggtgaactgt
   101221 ttgtgcagtt taacttccgc ttcagcaccg aactgactga tgagatgatc aaagcgcagg
   101281 tacttgccct gcttgaaaaa catcaactgc gctatacggt ggattggtgg ctttccgggc
   101341 agccattttt gaccgcgcgc ggtaaactgg tggatgcggt cgttaacgcg gttgagcact
   101401 ataatgaaat taaaccgcag ctactgacca caggcggaac gtccgacggg cgctttattg
   101461 ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa tgccactatt cataaaatta
   101521 atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg tatgtatcaa cgtatcatgg
   101581 aacagctcgt cgcgtgatga gtggttctgc aagaggaaat aagcatggac tggctggcta
   101641 aatattggtg gattctggtg attgtctttt tggtaggcgt cctgctgaac gtgattaaag
   101701 atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa gccggagctt cccccgcatc
   101761 gtgatttcaa cgataagtgg gacgatgacg acgactggcc gaaaaaggat caaccgaaga
   101821 agtaagctga gtgctcaacc gtaggccgga taaggcgtta acgccgcatc cggcaatggt
   101881 gaacgatccc tgatgcgacg ctcacgcgtc ttatcaggcc aggtaatctg tcttttacat
   101941 catttcaatc acatcatcat cgccaggctt accgccactt agcgcttggt cgaaataatg
   102001 tttcggaatg gtataacgca agtgatccag ggcaaattgc atactgcgat tgtcgatcgc
   102061 atggcctaaa tcctccacga tatccagcgt cacgtcacca ccggcactga ttaacgcttc
   102121 ctgcgccgca actgcatgcg ccagatcgat aaccggatct tcaccaccat gaatcaagtg
   102181 aattgtcgtg gctgtagacg cggtctccgg caggctcgca tagcgtccgt taaaggcaat
   102241 gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc agcgccataa tcgcgccttg
   102301 tgagaaacca attagcgccg tggcgttagc ccctactccg ctctgtttct gccagtagcg
   102361 taccgtttca ataaacgtcg gcatgatggc atccacacgc gcctggcgat tatcttccgt
   102421 aataccctgc actgaaaacc actgacgccc cgccggatta ccgctcggct ccgcgccacc
   102481 gacactcacc accagcgcat caggaaacag cggtgcaaac cagctgccta tttcccccat
   102541 tgccaccggg ttatccccga caccgtggaa aagcagtaac aactgttgtg caggtttatc
   102601 cgggctttga acaacaaaat ggtcatgttt catggcgaac tccttaactg atgtcattaa
   102661 ttttacgccg ctgactcaca atgaccatgg gagaaaattg aaggagtcag tgaaaaaatt
   102721 gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac ctcatttagt gcgaacagcg
   102781 cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt gcggcctgaa agtttaagtg
   102841 tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg cccacgcagc gccggtaatg
   102901 ccagttcact ggtttgtagc agacgcaata agcaacctaa cgatgttaat agcggacgat
   102961 gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc agaaaggatg gcatcgttta
   103021 gtggatcaac aggaagcatt tcgccattcc actgcgcgag agcttgcgca tcgcgacgta
   103081 aacggtaatg ctcacgttca gccagctgtt tacccgcatc actcatcggt aacagcgcca
   103141 tcgccgtata gcaaccgctg ctggcttccc gatgattacc catccgcacc agcacaaaac
   103201 cgcagcgttg ccagaaacgc cataactccc cagtgtaacc aaaactcacc gaaagatagt
   103261 cgaggtcatg cgtatattgc aaagctccag caataagttg tcgccctgtg ccttcccgct
   103321 gacgagccgg atgaactgct atccggctaa cccgccttcc gcgcaatgtc gccgccagtg
   103381 gattgctgcc gtgcgccgcc agcgactggg ccaccagatt accccgcggg cgacgaaaac
   103441 ctgcccatac cgcctgactg agttcttgag ataatccgcc ctcatccacc agccacaacg
   103501 ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg ttgccctggt gcatccatca
   103561 tccggcgtaa atccagcggc gaggtccggt agtgcgcacc agacaataac tgataaacct
   103621 ttaacggcgt ttctggctcg cttcgccata acgtttgttc aaatgcggaa atgacgatat
   103681 tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac cagtgcctca ctaaccattt
   103741 tttccagcgg gcatccctgt gcccagcgga tcggctgttg cagttcaaaa cggtgtaaat
   103801 gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc ggtaccttcg tagccctgca
   103861 ccgtagtggt taacaacgtt cgaggaaaac gcgataccag ttgatgcaac aacggcgcag
   103921 gtatggctgc ggcttcgtcg accaccagcc agtcggcttg ctcatcgctg gctaacaagg
   103981 catccggtgc aataaagcga aacttctcgc ccgcaaattg tgccagtaca tccgttgccg
   104041 cttttgcggg cgcggtgaca atcgcactgc ccgcaatacg agaaatgagt tgccctgcca
   104101 acgccgactt accgcgccca cgcgcagccg ttaccgctgc cacgcccggc ggcatggtca
   104161 gtagctgctg taagagttgc tgttgttctg gttgtggtgc gccggtcgcg gggtgccagt
   104221 cagtacgggg agtaaaatgc gccaacgaga acggctggtt ttgccgccag aggatagcgt
   104281 cgttatccgc cgtaagtacg cgtttgagat gctggacaaa atgcggcgtc gcaatagggt
   104341 cagggcaatc actccagcgc agcgagtcgg catcaggttg gttttcccac tcttcccata
   104401 cagggagtaa caaaaccagc cagcttcccg ctttcaacgt tccgctaagt gctgcaaagg
   104461 cagcggcatc aaagccgtgg cgggcgtcga ataccgcatg ccggaactcg cgcccaagta
   104521 aagtttgtag cgccgagggt gaacagtggt tttcagcatc tagctgcggc gaaatccaca
   104581 gccagtcgcc aggtaaggca tcacgcaact taagagcatg atcaaaacac caaccctctt
   104641 ccccgctcaa caccagcaag cggcagatcc cttcacgttt catttgcgct gttaatgtgt
   104701 aaagcgcagt cagttcagcc atccctgccc cggaaattaa atgcttttac caaaagtatt
   104761 gcattgtgcc ggatcgccgc tgtcgaaacc acgtttaaac cagctgtagc gttgctgaga
   104821 agtgccatgt gtgaaactgt ctggtactac tcgcccctga ctttgctgtt gtaaacggtc
   104881 atcgccgatg gcctgcgccg cattcagcgc ctcttccaga tcgccagttt ccagaacgcc
   104941 ttgctgctgc atactatgcc cccagacacc ggcaaaacag tcggcctgga gttccatacg
   105001 cacagataag cggtttactt ccgcctgcgt cgcgttttgt tgcagttgac gaactttcgg
   105061 ctcgatgcct aacagtttct gcacatgatg accgacttca tgggcgataa cgtacccctg
   105121 ggcaaaatcg ccatccgcgc caagtttgtc tttcatgtca tcatagaagg agagatcgat
   105181 ataaactgtg ccatccgccg ggcaatagaa cggccccata atggactggc ccgccccgca
   105241 gccggtgcgc gtcattccac ggtacatgac cagcttcggt tgctgatagg tcttacccat
   105301 cttttcgaac tgttgtcccc aggtgtcttc cgtggttgcc tgaatcaccg aggtgaattt
   105361 tgcggcttcg tcttcattag ggctaattga ccgcgttgat tgttgttgag aaaccggctg
   105421 cccggtcatc aacccggtta aatcaacgcc atagtagcct gcaaccagca cgactatcag
   105481 taaaatcagc ccgcctttac cgctcggcag acggaaaccg ggaccgccca ttgacggacc
   105541 gccagagctg ttgcgcctgt cttcaacatt gtcactttca cgtcgccctt gccaacgcat
   105601 agatacctca acaatatatt cattatagaa atgatcgtag gtggttcaga ggaagattac
   105661 cacaggaaag cgggatgaaa tggcggagga atgagcgttc aatgcatata ttccaagagc
   105721 ggttgtcttg ccgtgccagc tgcacggcaa gatgatgaac agaaaaatca gtccagctgt
   105781 acacccaggc ggcgggcgac ggcttcatag gcttcgatca ggccaccgag gctctggcgg
   105841 aaacggtctt tgtccatttt ctccagtgtt tctttgtccc acaggcggct gccgtccggg
   105901 gagaactcat cgcccagtac cacttcacct ttgtacagac caaactccag cttgaagtcg
   105961 accagaatca gaccagcatc atcgaacagt tttttcagca cgtcgttcgc tttgtaggtc
   106021 agctctttca tacgcgccag gttctctttg ctcacccagc caaaggtttc gcagtaagat
   106081 tcgttgacca tcggatcgtg catggcatcg tttttcagga acagatcgaa cagcggcggg
   106141 ttcagatcaa taccttcttc gattccaaga cgtttcacta gagagccagc agcacggtta
   106201 cgcacgacac actcaaccgg caccatatcc agctttttca ccagacattc ggtatcggag
   106261 agcagacgct ccatttgagt cgggataccc gcttcagcca gtttgctcat aatgaagtag
   106321 ttgaacttgt tgttcaccat acctttgcga tcaaactgct caatgcgcgc gccatcccct
   106381 gctgacgtat cattgcggaa ttcgagcacc aacaggtccg ggttttccgt gctgtatacg
   106441 gttttcgctt taccacgata caactcagct tgcttttgca tctttatcac tcctgggtgt
   106501 gaattaacgt tttaaaatct tttgctgtct ggtgtgccgg atgttttgtc ggatgcggcg
   106561 tgaacgcctt atccgaacta cgtccgacaa acaaatttcg tgcgaattac cgctaaaatc
   106621 gcgttttcct gccgacgcac acgtttgcgt atcatatcag aaaaaagggc cggatgattc
   106681 cagccctgta tttttacttg ctaaacgcag cctggaagac agctaccagc gcgtcgttct
   106741 gactctgagt cagagtatga cctttcggat cgatgaactg taggctgctg cggttatcta
   106801 aatcgccaac ctgcagttta tagtcaccgg atgccaggcc tggatcgctc gcgcccagtt
   106861 cctgccagtc gctgtcagac agcggcttat aagttacggc catgttgccc tgcgaacggg
   106921 tgctgtcggt cactttcatg cccacttttt ccagcgccgc tggcagacgt tgccaaacca
   106981 cattgaacgg cccgcgtacg accagcattg gtaaaccggt gtcatcagct gcactttgta
   107041 cgtccatagt ggtggaggca cgattttgcg cagcgttcgc ggcgtcagtg gcagatttat
   107101 ccagaccggc ggaaataacg ttcatcatct ccgtgctgta acgctgcatg gaagccgcgt
   107161 ctgcaaccgg tttgcccgcc tgttccaggt tcagcagttt aaccgtaacc gcctgctgat
   107221 aaccctgcgg cttaacagag atttgataac gaccacgata ctgctcgtct tcgtccagac
   107281 ggttccattg tacccaatcg gtggtcagtg tctgaccagc atcatcacgt tgggtgatgg
   107341 tgtagttttt cgcctgcagc acgctaacca cctgcggcca cagagtattg ccacgaccat
   107401 tttccaccag caatgaagcg gtatcgcccg tgaactgggt acgcgcgcca gaaaccagtg
   107461 ccaacggctg ggctggtgga cgaatgtcca gcgccttacc gacagcacca ctaccgttgg
   107521 tcaccgggat tgcataatca ccggaggtca ccggcaaaat cattccagcc ggggcatgaa
   107581 gctccgcaag cggtgccgct tccaggtagg cttcatcacc actgacctga cgcttatagc
   107641 gtgagtcaga actacaggca gcgagtaata aaacaagcga aacacccgca acctttgcca
   107701 ggcgcgactt ttgaacagag taagccatca aatctcccta aactttacag caaaccggca
   107761 tgcttaagcg ccgctctgac cgtctcacga ccactgtcgg tgattggtgt cattggcagg
   107821 cgcagcgtat cggtcgccac aagacccagt tccttacatg cccatttcac cgggattgga
   107881 ttgggttcga caaatagttt gttgtgtaat ggcatcagac gctgattaat aacgcgtgcc
   107941 tcggcaaaat gcccttctgc tgccagtttg cacatctggg ccatatcacg cgctgcgacg
   108001 ttagccgtaa cggaaataac tccatgaccg cccaattgca tgaagtccag cgcgctcgca
   108061 tcatcgccgc tcagcagaac aaaatcatct gaaaccagct ctttgatctg gtttacacgc
   108121 gttaagttcc ctgttgcctc tttgattccg ataatatttt ttactttcgc cagacggccc
   108181 accgtttccg ggagcagatc gcagccagta cgggacggca cattatacag aatttgcggc
   108241 aggtcagtat gctcagcgat ggctttgaaa tgctgataca aaccttcttg cgacggacga
   108301 ttgtagtaag gggttaccgt caggcagccg acgataccac tgtcattgaa gcgctgcgtc
   108361 aggctaatgg cttccgcagt agcgttagcg ccggtcccgg caattaccgg aatgcgccca
   108421 tcagccagat ccagcgtcat catcaccaca tcagcatgtt cgtcatgatt taaggtagcg
   108481 gactcgccag tggtgccaac agaaacgatc gccgaagtac cgctggcgac atgataatca
   108541 atcagttttt tcaagctagc ccgacagaca ttaccttttt catccatcgg agtaacaatc
   108601 gcgacaatac ttcccgtgaa catgggccat cctctgtgca aacaagtgtc tcaatggtac
   108661 gtttggtatg gcattaaaag caagcagaca gaaccgttct gattgttgta tgcatgattt
   108721 ttttatgctt tccttaagaa caactcaccc cttaaaggaa taaccagttt gacactgtca
   108781 tcgcaacatt atctggtgat cactgcgttg ggtgccgatc gccctggaat tgtgaacacc
   108841 atcacccgtc atgtcagtag ttgcggctgt aatattgaag acagtcgcct ggcgatgctg
   108901 ggagaagagt tcacgtttat catgctgctt tccggttcat ggaatgccat cactctgatt
   108961 gaatcaacgt taccgttgaa aggtgccgaa ctggatcttt taatcgtgat gaagcgcacg
   109021 acggcgcgtc cgcgtccgcc aatgccagca tctgtctggg ttcaggtcga tgttgcagac
   109081 tccccgcatt taattgaacg cttcacagca cttttcgacg cgcatcatat gaacattgcg
   109141 gagctggtgt cgcgcacaca acctgctgaa aatgaacggg ctgcgcagtt gcatattcag
   109201 ataaccgccc acagccccgc atctgcggac gcagcaaata ttgagcaagc gttcaaagcc
   109261 ctatgtacag aactcaatgc acaaggcagt attaacgtcg tcaattattc ccaacatgat
   109321 gaacaggatg gagttaagta atgaatccac tgaaagccgg tgatatcgca ccgaaattta
   109381 gcttgccgga tcaagacgga gaacaagtta atttgaccga cttccaggga cagcgtgttc
   109441 tggtttattt ctacccgaaa gccatgaccc ccggctgtac cgtacaggcc tgcggcttac
   109501 gcgataacat ggatgagttg aaaaaagcgg gcgttgatgt gctgggtatc agcaccgata
   109561 aacccgaaaa actctcccgt tttgcggaaa aagagctgct taactttacg ctcctgtctg
   109621 atgaggacca ccaggtgtgc gaacaattcg gcgtctgggg tgaaaagtcc ttcatgggca
   109681 aaacctacga tggtattcat cgcatcagct tcctgattga cgctgatggc aaaatcgaac
   109741 atgtctttga cgatttcaaa accagcaatc accacgacgt tgtgctgaac tggctgaaag
   109801 aacacgcctg attactttgc tccattccgt gctggctgcg cttgcggcca gcatacctca
   109861 cttctcgtga tcaagatcac attctcgctt tcccctgcga cacgggtgtc gaatccattt
   109921 tttgctgaac gttaatgacc atcatttttg taccgttcag aatccagtta atacataact
   109981 tattgaatag attgagttaa tcagaatggc atcctttata caatatgaaa tgcaatgttt
   110041 catatcattt tcaaggagcc gacatgaacc gctttgtggt ggccgaacca ctgtggtgta
   110101 caggatgtaa tacctgtctc gctgcctgtt cggacgtgca taaaacgcaa ggtttacagc
   110161 aacacccgcg cctggccctg gcgaagacgt caacaatcac tgcccctgtc gtgtgtcatc
   110221 actgtgagga agccccttgc ctgcaggtct gcccggtcaa tgccatctct cagagggatg
   110281 atgcgatcca actcaacgaa agcctctgta ttggctgcaa gctttgcgcc gtggtctgcc
   110341 catttggcgc aatcagcgct tcaggaagcc gtccggtgaa tgcccatgcg caatatgttt
   110401 ttcaggctga aggctcactc aaagacggcg aagaaaacgt gccgccccaa catgcgttgc
   110461 tgcgctggga accgggtgtc cagaccgtcg cggtgaaatg cgacctgtgt gatttcttgc
   110521 cagaaggtcc ggcctgcgtt cgcgcttgcc cgaatcaggc gttacggctg atcaccgacg
   110581 atagcctgca acgtcagatg aaagaaaaac agcgccttgc cgcaagctgg tttgccaatg
   110641 gcggggagtc tcccctttcc ctcactcagg agcaacacta atggatgccc tgcaattatt
   110701 aacctggtcg ctgatcctct atctgtttgc cagtctggct tcgctgtttt tactcggtct
   110761 ggacagactg gctattaagc tttccggcat cacatcgctg gtgggcggcg tgattggcat
   110821 catcagcgga attacgcaat tacatgctgg tgtaacttta gtcgcccatt ttgccacccc
   110881 ttttgacttt gccgatttaa ccctgcgaat ggatagcctc tcggcattta tggtgctagt
   110941 tatctccttg ctggtggtgg tttgttcgct ctattcattg acttatatgc gcgaatacga
   111001 gggcaaaggc gcggcggcga tgggcttctt tatgaatctt ttcatcgcat cgatggttgc
   111061 cctgctggtg atggacaacg ctttttggtt catcgtgctg tttgaaatga tgtcgctgtc
   111121 ttcctggttt ctggtcattg ccaggcagga taaaacgtcg atcaacgctg gcatgctcta
   111181 cttttttatc gcccacgccg gatcggtgct gattatgatc gccttcttgc tgatggggcg
   111241 cgaaagcggc agcctcgatt ttgccagttt cgcacgcttt cactttctcc ggggctggcg
   111301 tcggcggtgt ttctgctggc ctttttcggt tttggcgcga aagccgggat gatgccgttg
   111361 cacagctggt tgccgcgcgc tcaccctgcc gcaccatcgc acgcttcggc gttgatgtct
   111421 ggcgtaatgg tcaaaatcgg tattttcggc atcctgaaag tagcgatgga tctgctggcg
   111481 caaacgggtt tgcctctgtg gtggggcatt ctggtgatgg cgatcggcgc aatctccgcg
   111541 ctcctgggcg tgctatatgc gctggcggaa caggatatca aacggctgct ggcctggagt
   111601 accgtcgaaa acgtcggcat tattttgctg gcagtcggtg tggcgatgga cggtctgtca
   111661 ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg cactgtttca tctgctcaac
   111721 catgcgctgt tcaaagggct gctatttctc ggcgcggacg cgattatttc gcgtttgcat
   111781 acccacgaca tggaaaaaat gggggcactg gcgaaacgga tgccgtggac agccgcagca
   111841 tgcctgattg gttgcctcgc gatatcagcc attcctccgc tgaatggttt tatcagcgaa
   111901 tggtacacct ggcagtcgct gttctcacta agtcgtgtgg aagccgtagc gctacaactt
   111961 gcgggtccta ttgctatggt aatgctggca gtcactggtg ggctggcagt aatgtgcttc
   112021 gtcaaaatgt acggtattac tttctgtggt gcgccgcgca gtacacacgc tgaagaggca
   112081 caggaagtgc caaatacgat gatcgtcgcc atgctactgc tcgcggcact ctgcgtatta
   112141 attgcgctta gtgccagttg gctggcaccg aagataatgc atattgccca tgcgtttacc
   112201 aatacccctc ccgtcactgt cgccagcgga atagcacttg tacccggcac gtttcataca
   112261 cgggtcactc cttcattact gttgctgtta ctactggcga tgcctttgct gccaggcctt
   112321 tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc gcacaggaga tgcctgggca
   112381 tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca atggcgtgat gcagccgctg
   112441 cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc tcgaccctac gctgaggcta
   112501 aataaaggtc ttgcgcacgt caccgccagg gctcagagca cagaaccctt ctgggatgag
   112561 cgggtgatcc gccccatcgt gagcgccacc caacggctgg tcaaagaaat acagcatctg
   112621 caaagcggcg actttcgtct ctattgcctg tatgtggtcg ccgcactggt tgtgctgcta
   112681 atcgctattg ccgtctaagg aaatcaccat gagacaaact ctttgcgacg gatatctggt
   112741 catttttgcg ttagcacagg ccgtgattct gctgatgcta accccacttt ttacgggtat
   112801 ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg ccggggatct ggcaggatta
   112861 tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg ccgacatctt caggtctgat
   112921 gttccgcctg atgccgtggg tattaatcag cagcatgctg gtgctggcga tggccttacc
   112981 actatttatt accgtttccc cttttgcggg cggcggcgat ctgatcaccc ttatctatct
   113041 tcttgccctg tttcgttttt tctttgctct ttccgggctg gataccggaa gtccgtttgc
   113101 gggagtcggt gccagtcgcg agttgacgct cggcattctg gtcgaaccaa tgcttattct
   113161 ctcactgctg gtattggcgc tgatagcagg ttccacgcat atcgagatga tcagcaatac
   113221 gctggctatg ggctggaact cgccgctaac caccgtactg gcgttactgg cctgtggttt
   113281 tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt gctgaagcag aacaggaatt
   113341 acaggaaggc ccgctgaccg aatattccgg tgccgggctg gcgctagcga aatgggggct
   113401 ggggctgaaa caggtcgtga tggcagcact gtttgtggcc ctgtttctgc cctttgggcg
   113461 cgcgcaagaa ctttctctcg cctgcctgct gacttcactt gtcgttacgc tgctcaaggt
   113521 tttgctgatt tttgtactgg cctcaatcgc agaaaacacg ctggcacgcg ggcgtttttt
   113581 actcattcac catgtgacct ggcttggctt cagccttgct gcgcttgcat gggtcttttg
   113641 gttaaccggt ctgtaaggag cactgacgga atatggaaaa tcttgctctg acgacgttaa
   113701 tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg gccgccgaat
   113761 ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt gtcgctgatc tccgcgtttt
   113821 atcaggccga taaagttgcc gtcacgttga cgttggtcaa cgtgggggat gtggcattat
   113881 ttggcctggt cattgatcgc gtgagtacgc tgattctgtt tgtggtggtg tttctcggtt
   113941 tgctggtcac gatctactcc acgggttatc tgacggataa aaatcgcgaa cacccgcata
   114001 acggcacgaa tcgttattac gcatttttgc tggtgtttat cggcgcgatg gcgggactgg
   114061 tactctcctc gacgctgctc ggtcagttgt tgttttttga aattacgggc ggctgctcct
   114121 gggcgttgat cagttattac cagagcgata aagcgcagcg ttcagcacta aaagcgttgc
   114181 ttatcaccca tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga
   114241 ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt
   114301 atggcggcat cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc
   114361 taccggacgc aatggaagcg ccaacaccga tcagcgccta tctccacgcc gcatcgatgg
   114421 tgaaagtggg cgtttacatt tttgcccgcg ccattatcga cggcggcaat atcccgcatg
   114481 tgattggcgg cgttggcatg gtcatgacac tggtcaccat tctttatggc tttctgatgt
   114541 atttgccaca gcaggatatg aagcggttgc tggcctggtc gaccatcact caacttggct
   114601 ggatgttctt cggcttgtcg ctctccatct tcggctcgcg gctggcgctg gagggtagca
   114661 tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt tttccttgta gcaggtgcgc
   114721 tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg tggcgtattg cacaccctgc
   114781 cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat taccggtgtg ccgccgttca
   114841 acggcttctt cagtaaattc ccgctgtttg ccgccggttt tgcgttgtca gtggagtact
   114901 ggatcctgct gcccgccatg attctgctga tgattgaatc ggtcgccagt ttcgcctggt
   114961 ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag cgaggccgtc gccgatgccg
   115021 caccgctgcc agggtcaatg cgcctggtgt tgattgtact gattgtgatg tcgctgattt
   115081 ccagcgtaat cgccgcgacc tggttgcagt aaggagatga tgaatgaccg gttctatgat
   115141 cgtaaataat ctggcgggac tgatgatgct gacatcgctg tttgtgatta gcgtcaaaag
   115201 ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg gtgctggtgt ctattttcgc
   115261 cactctctcg tgcctgttcg ccacagagca actgctgatc tggtccgcca gcgcctttat
   115321 caccaaagtg ctgctggtac cgttaatcat gacttacgct gcacgaaata ttccccagaa
   115381 catcccggaa aaagcgttat tcggtccggc aatgatggca ctgctcgcgg cgttaattgt
   115441 cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg atggctaccg ggctgaaacc
   115501 ggcgctggcg gtagcgttag gtcattttct gcttggcctg ctgtgcattg tcagccaacg
   115561 caatatcctg cggcaaattt ttggttactg cctgatggaa aacggctccc atctggtgct
   115621 ggcgcttctt gcctggcgag caccggaact ggtggaaata ggtatcgcta ccgacgccat
   115681 cttcgccgtc attgtgatgg tgttactggc aagaaaaata tggcgtaccc acggcacgct
   115741 ggacgtgaac aacttgaccg cgctgaaggg ataatgagat gagttattct gtgatgttcg
   115801 ctttactcct gctcacgccg ctgctttttt cgctgctctg ttttgcctgc cggaaacggg
   115861 ggctctctgc gacttgtacg gtgactgtat tacatagctt agggatcaca ctgctgctga
   115921 ttctagcact ctgggtggtc caaactgccg ctgatgcagg agaaatattc gctgcgggac
   115981 tgtggcttca tattgatggt ctgggcggtt tgttcctcgc cattcttggt gtgattggct
   116041 ttctcaccgg tgtttactcg attggctaca tgcgtcatga agtggcacac ggcgagcttt
   116101 cacccgttac actgtgcgat tactacggtt tcttccatct gtttttgttc accatgctgc
   116161 tggttgttac cagcaataac ctgattgtga tgtgggcggc gatcgaagcc accaccttaa
   116221 gctcggcgtt tctggtaggc atttacggtc agcgttcatc gctggaatca gcatggaagt
   116281 acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt cggtaccgtg ctggtatacg
   116341 ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat attctggagc gaggttctta
   116401 agcaatcgtc cttgcttgac ccaacattaa tgctgttggc ctttgtgttt gtgctaattg
   116461 gctttggtac caaaaccggg ctatttccca tgcacgcctg gctgccggat gctcacagtg
   116521 aagcgccgag tccggtcagc gccctgctct ccgccgtatt gctgaactgc gcgctgttgg
   116581 tgctgattcg ctattacatc attatttgcc aggccatcgg cagcgatttc cccaaccggt
   116641 tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc atttttcatt ctggtacagc
   116701 gggacattaa gcgtctgctg gcgtactcca gcgtggagaa catggggctg gtcgcggtgg
   116761 cgctaggcat tggcgggccg ctgggaattt ttgccgcgct gctgcacacc ttaaaccaca
   116821 gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt actgctcaag tacggcacgc
   116881 gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc atttaccgcc gtgctgtttg
   116941 gcggcggtgc gctggcgctg gcgctggcgc tggcagggat gccgcccttc aacatttttc
   117001 ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg attatcgtcc
   117061 tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct gcgcgggtgt
   117121 taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg accacctcgc
   117181 caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca caacctgtca
   117241 tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac ctgcctgcac
   117301 aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg gagaaacaca
   117361 gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc tcgccgccct gaaaacgcag
   117421 ttccccggcg cggtgctgga tgaagagcga caaacgcctg aacaggtcac cattacggtg
   117481 aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga tggctggctt
   117541 ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt ttattatgcc
   117601 ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt cgatgccgac
   117661 agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg cgagcgagaa
   117721 attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg cctggtgttg
   117781 cccgatgact ggccggaaga tatgcatccg ctgcgcaaag atgcgatgga ttatcgactg
   117841 cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg caacagcgat
   117901 gcgcgggtga tccctgtcgg cccgctgcat atcacctcct atgaaccggg tcacttccgc
   117961 ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta tgtccatcgc
   118021 ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt cttatcggac
   118081 cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc ggttgaaaat
   118141 gcactgggga ttgaggtgcc gcaacgagca catactattc gctcgattct gctggaagtc
   118201 gagcggctac acagtcattt gctcaacctt ggcctctcct gccattttgt tggttttgat
   118261 accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc ggaattgctg
   118321 accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg cgatattctc
   118381 aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga cgtgtcggag
   118441 ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg cattggcatt
   118501 ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg cgcagtggtt
   118561 ttgcccgtga tttgcgcttt gatcacccct acgccgacta cggcaatatt ccaaaaacac
   118621 tgtttacctt taccggcggc gatgttttct cccgcgtgat ggtccgtgtc aaagagacgt
   118681 ttgattcgct ggcaatgctg gaatttgccc tcgacaacat gccggatacc ccactgctga
   118741 ccgaaggctt tagctataaa cctcacgcat tcgcgctggg ctttgttgaa gcgccacgcg
   118801 gtgaagacgt gcactggagc atgctcggtg ataaccaaaa attgttccgc tggcgctgtc
   118861 gtgccgccac ctacgccaac tggccggtgt tgcgttacat gctgcgcggc aataccgttt
   118921 ctgacgcacc gctgattatc ggtagccttg atccctgcta ctcctgtacc gaccgtgtga
   118981 cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgct gtataaagag atcgaacgct
   119041 acggcattga tcgtaaccgt tcgccgctga agtaaggaca gaagatgctg aagttactga
   119101 aaactattat gcgcgccgga accgcgacgg tgaaatatcc cttcgcgcca ctggaggtca
   119161 gcccaggctt tcgcgggaaa ccggacctga tgcccagcca atgtattgcc tgcggtgcct
   119221 gcgcctgtgc ttgtccggca aatgcgctga ctatccagac cgacgaccag caaaattcgc
   119281 gcacctggca gctctatctg gggcgttgta tttactgcgg acgttgtgaa gaagtgtgcc
   119341 cgaccagagc catccagctt accaataact ttgaactgac cgtcacccat aaagccgatc
   119401 tctataccca cgcgacgttc catctacaac gttgcagccg ttgcgaacgc ccgtttgccc
   119461 cgcaaaaaac cgtcgcactg gctgctgaat tgttggcaca gcaacaaaat gctccacaaa
   119521 accgcgaaat gttatgggcg caagcgagcg tctgcccgga gtgcaaacaa cgcgcaacgc
   119581 tgatcaacga cgatacagat gtaccgctgg tggctaagga gcagctatga gtccagtgct
   119641 tacgcaatat gtcagccagc ccatcacgct ggacgagcaa acgcaaaaga tgaagcggca
   119701 tttgctacag gatatccgtc gctcggctta cgtttatcgc atcgattgcg gcggctgcaa
   119761 cgcctgtgaa atcgaaattt ttgctgccat cacccccgta ttcgacgcag aacgttttgg
   119821 cattaaggtt gtttcatcac cgcgtcacgc cgatattttg ttatttactg gcgcagtcac
   119881 ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct gcccccgatc ataaaatctg
   119941 tgtttcctac ggcgcgtgcg gtgtcggcgg cggtattttc cacgatctct acagcgtctg
   120001 gggcggcagc gacaccattg tccccattga tgtttggatc cccggctgcc cgccaacacc
   120061 ggccgccacc attcacggtt tcgccgtggc gctcggtttg ctgcaacaga agattcacgc
   120121 ggtggattat cgcgatccca ccggggtgac tatgcaaccg ttgtggccgc agatcccgcc
   120181 atcacagcgt atcgccattg agcgagaagc gcggcggctg gcgggctatc gtcaggggcg
   120241 agaaatttgc gatcggctcc tgcgccattt aagcgacgat cctacaggaa atcgggttaa
   120301 cacctggttg cgcgatgccg acgatccacg tctcaatagt atcgttcagc aactctttcg
   120361 cgtactccgg gggttacatg actgaagagt gcggggaaat tgttttctgg acgctgcgaa
   120421 aaaagtttgt cgccagtagc gacgagatgc cggaacacag ctctcaggta atgtattact
   120481 cgctggctat cggtcatcac gttggcgtga ttgattgtct gaatgtcgcc ttccgctgcc
   120541 cactgacgga atacgaagat tggcttgcac tggtcgaaga ggagcaagcc cgacgtaaga
   120601 tgctgggggt gatgactttt ggtgagattg ttattgacgc cagccacacc gccctgttga
   120661 cccgggcatt cgcgccactg gcagatgacg cgacgtctgt gtggcaagcg cgtagcattc
   120721 aattcattca tctgttggat gaaattgtgc tggaaccggc tatctatctg atggccagaa
   120781 aaattgcgtg agaaggattt ctcattaata aggactgttg atggctatgt cagacgaagc
   120841 gatgtttgcc ccgccgcaag gaataacaat tgaagcggta aacggaatgc tcgcggagcg
   120901 gttagcgcag aaacacggca aggcgtcttt attacgcgcc ttcatcccgc tgccgccgcc
   120961 gttcagcccg gtacaactta ttgaactgca tgttctcaaa agcaacttct attaccgcta
   121021 ccatgatgat ggcagcgatg tgacggcaac aacagagtat cagggcgaga tggtcgatta
   121081 ttcgcgttac gccgtccttc tcggcagtag tggaatggcg gagctacgct ttattcgtac
   121141 ccacggcagc cgttttactc cccaggattg cacactgttt aactggctgg cacgcattat
   121201 caccccggtt ctgcaatcat ggctcaatga tgaagcacag caggtggcgc tgcgtttgct
   121261 ggagaaagat cgcgatcatc atcgggtact ggtggatatc actaatgcag tgctgtcaca
   121321 tcttgatctc gacgatctga tcgctgacgt cgctcgtgag atccatcatt ttttcggtct
   121381 ggcttcagtc agtatggtac tgggcgatca tcgaaagaac gagaagttca gcctgtggtg
   121441 cagcgatctt tctgcctcac attgtgcgtg tctgccacac aatatgcctg gcgacagtgt
   121501 attgctgaca caaacgctac aaacccgaca accgaccttg acgcaccgtg cagatgatct
   121561 gtttctctgg caacgcgacc cgttattact cttacttgca tctaacggct gcgaatctgc
   121621 gctccttata ccgcttacct ttggcaacca tacaccgggt gcattgttgc tggcgcatac
   121681 ctcttccact ctctttagtg aggaaaactg ccagctacta caacacatag ccgatcgcat
   121741 cgctattgcc gttggcaatg ccgatgcctg ccgtagaatg accgatttgc aggaaagttt
   121801 gcagcaagaa aaccaccagc ttagcgagca gctcctttcg aatctgggcg tcggtgacat
   121861 tatctatcaa agccaggcaa tggaagacct gctccagcag gtagatattg tggcgaagag
   121921 cgacagtacg gtgttgattt gcggtgaaac cggaaccggc aaagaggtga tcgccagagc
   121981 gatccatcaa cttagcccgc gacacgacaa gccgctggtc aaaatcaact gcgctgccat
   122041 ccccgccagt cttctggaaa gtgagttatt cggtcatgac aaaggggcgt ttactggtgc
   122101 gattaatacc catcgtggtc gttttgaaat tgccgatggc ggcacgttgt ttctcgatga
   122161 aattggcgat ctgccgttag aacttcagcc taaactgctg cgcgtattgc aggaacggga
   122221 gattgagcgt ctcggcggga gtagaacgat cccggtaaat gtcagagtca ttgccgccac
   122281 caaccgtgat ttgtggcaaa tggttgaaga tcgccagttt cgcagcgatc tcttttatcg
   122341 cctgaatgtc ttcccactgg aattgcctcc gctgcgcgac cgtccggaag atatccctct
   122401 tttagcaaag catttcacgc aaaaaatggc gcgccatatg aatcgttcaa ttgacgccat
   122461 cccgaccgag gcgctacgcc agttgatgtc gtgggactgg ccgggcaacg tgcgcgagct
   122521 ggaaaacgtg attgagcgag cggtactgct gactcgcggt aacagtctga atttacatct
   122581 taatgtccga caaagccgtt tactgccgac gctaaatgaa gattcagcgc ttcgcagttc
   122641 aatggcgtag ttgctgcacc cgacgacgcc agagaatgac gaagaagaac gtcagcgcat
   122701 tgttcaggta ttgcgagaaa ccaatggcat tgttgccggg ccccgtggcg cggcgacacg
   122761 attagggatg aagcgcacca cgctgctgtc acgaatgcag cgtctgggaa tctcggttcg
   122821 cgaggtgttg taatctgctt ttgcaggagt atgcatgaga aacaaactct ctttcgactt
   122881 gcagttgagc gccagaaaag cggcaatcgc tgaacggatt gccgcccata aaattgcccg
   122941 cagtaaagtg tcggtctttt taatggcgat gtccgctggc gtgtttatgg cgatgtccgc
   123001 tggcgtgttt atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc
   123061 ttcataggca ttaacccatc tggtgggcgg cctttgcttt acacttggct ttattttgct
   123121 ggcggtttgc ggcaccagcc tgttcacctc gtcggtaatg acggtaatgg caaaaagtcg
   123181 gggcgttatt agttggcgaa cttggctgat taacgcactt ctggtggcct gcggtaatct
   123241 ggcaggtatt gcctgtttca gtttgttaat ctggttttcc gggctggtga tgagtgaaaa
   123301 cgcgatgtgg ggagtcgcga ttttacactg cgccgagggc aaaatgcatc atacatttac
   123361 tgaatctgtc agcctcggca ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag
   123421 ttattgcgga cgttcgttat gcgacaaaat cgtcgccatg attttgtcca tcaccctgtt
   123481 tgtcgccagt ggctttgagc actgtatcgc caatttgttt gtgattccgt tcgccattgc
   123541 cattcgccat ttcgccccta cctccttctg gcaactggcg cacagtagcg cagacaattt
   123601 tccggcactg acggtcagcc attttattac cgccaatctg ctcccggtga tgctgggtaa
   123661 tattatcggc ggtgcagtgc tggtgagtat ttgttatcgg gctatttatt tacgtcagga
   123721 atcctgaaaa atcagcccgg cgaaacagtt cgtcgggcta tttatttacg tcaggaatcc
   123781 tgaaaaatca gcccggcgaa acagttcgtc gggctgaaga ttattcttgc gcgatttgcc
   123841 catcgggcca ggcgtggatc acggctttga tcagcgtcgc caatggaatg gcgaagaata
   123901 cgccccagaa tccccacaaa ccaccgaaga tcaccaccga taaaataatc accagcggat
   123961 gcaggttaac cgcttcggag aacaacaccg gtactagcag gttgccgtcc agcgcctgaa
   124021 taatcagata caccgcgaag cagctccaga attcagtacc tgcgccaaac tggaatagcg
   124081 ccacgccaac caccggaatg gtcaccacaa atgcgccaat gtacggaata agaaccgaga
   124141 agccgaccag cactgccagc agcagcgaat agttcagccc gaagagcaag aaccccagcc
   124201 aggtggcgat ccccaccacg atcatctcca gcactttgcc gcggatatag ttggtgattt
   124261 gttgattcat ctccttccac acctgtcctg ccagcccacg gttacgcggc agcacccggc
   124321 gaacggcgtt cagcatctgc tctttgtctt tcagcaggaa gaagaccatc aatggcacca
   124381 gcaccagata gacggctatg gtcagcaaac cgaccagcga ggcgagggaa attttcacca
   124441 ccgaatcgcc catggtcagc atccgactgc gcatattttc ggccattgca tcaataatgc
   124501 ccgcgtccat taacaccgga tagcggcgcg gcaacgtggc ggcaaagtca gaaagcttat
   124561 tgagcatccc cggcatatcg cggattaagt agatgccctg ttgccaggcg ataggcagta
   124621 ccacgaacgc catcagtagc aatataccga caaaaaccac caatacaatc gacgtcgccc
   124681 agcggcggga gcagccaata gattgcaggc gcacggttgg ccattccagc aaataagcca
   124741 gcacaatagc caccagcaac ggagcaagca ggccactaaa gaaaaagata atgccaaatc
   124801 cggcaactaa aataaccagc aaggcaatcg cttccgggtc gctaaaacgg cggcgatacc
   124861 attgcatcaa catttcgagc ataaagacct tccctgaacc tcaagagcgg gattgcgatc
   124921 cgcaattgta tcgaaatgtc acaaaaaaga cttcgctttt tatgacggat tcaggaaact
   124981 gaaaagtcat ttgagtgggc taatcttcgc cgttacactc aaaggcggcg aggtgggaac
   125041 gatatttcac agtatcggtc aaatgactat cttcagaaat acaggataga ggttatgttc
   125101 aggcagttga aaaaaaacct ggttgcaacc ctcattgctg ctatgaccat tggtcaggta
   125161 gccccggcgt ttgccgacag cgcagacacc ttgccggata tgggaacctc cgcaggaagc
   125221 acgctttcca ttggtcagga aatgcagatg ggcgactatt atgtccgcca gctacgcggc
   125281 agcgcgccgt taattaatga cccgctgtta acgcaatata ttaattcgct ggggatgcgt
   125341 ctggtttcgc atgccaattc ggttaagaca ccgtttcatt tttttctgat caacaacgac
   125401 gaaattaacg cctttgcttt ctttggcggc aacgtggtgc tgcactctgc cctgttccgt
   125461 tattccgata acgaaagtca actggcttca gttatggcgc acgaaatctc ccacgtcacc
   125521 caacgtcacc tggcgcgagc gatggaagat caacaacgca gcgcgccgct gacctgggtc
   125581 ggcgcgttag gttctatttt actggcgatg gccagtccgc aggcggggat ggcggcgctg
   125641 accggtacac tggcgggaac gcgccagggg atgatcagtt tcacccagca aaatgaacag
   125701 gaagcggacc gcattggtat tcaggtgctg caacgctcgg gattcgatcc acaggcgatg
   125761 ccgaccttcc tggaaaaatt gctcgatcag gcgcgttact cctcgcgccc gccggaaatt
   125821 ttattgactc acccgttgcc ggaaagtcgt ctggcagatg cccgcaaccg tgctaatcag
   125881 atgcgcccga tggtggtgca gtcgtcggaa gatttctatc tggcgaaagc gcgcacactg
   125941 gggatgtata attccggacg taaccagctc accagtgatt tgctggatga atgggcgaaa
   126001 ggaaacgttc gtcagcaacg agcggcgcaa tatggtcgtg ctttacaggc gatggaagcc
   126061 aataaatacg acgaggcgcg taaaacgctg caaccgttac tggcggcaga acctggcaac
   126121 gcatggtatc tcgatctggc taccgatatc gatcttgggc aaaacaaagc caatgaggca
   126181 atcaatcgcc tgaaaaatgc ccgcgattta cgcaccaatc cggtgttgca gctcaacctg
   126241 gcgaacgctt atctgcaagg cggtcaacca caagaagcgg ccaatattct gaatcgctac
   126301 acctttaata ataaagatga cagcaacggc tgggatttgc tggcacaggc ggaagccgcg
   126361 ctaaataacc gcgatcagga gctggctgcg cgagcagaag gttatgcgct cgccggacga
   126421 ctcgatcagg ccatttcgct gttgagtagc gccagttcgc aggtgaaatt aggcagcctg
   126481 caacaagcgc gttacgatgc gcgcatcgac cagttgcgcc agctgcagaa acgctttaag
   126541 ccttatacca agatgtaatt cgcattaaag gaagaacaat gaccaaacag gtaaaaatct
   126601 accataaccc acgctgttca aagagccggg aaacgctgaa cctgctgaaa gaaaacggcg
   126661 tggagccaga agtggtgctc taccttgaga cacccgccga tgcggcaacg ctgcgcgatt
   126721 tgctgaaaat gctggggatg aatagcgccc gtgaattgat gcgccagaaa gaggatcttt
   126781 ataaagaact aaacctggct gacagttcgc ttagcgaaga ggcgctgatt caggcaatgg
   126841 tggataatcc gaagctgatg gagcgcccga ttatggtcgc gaatggcaaa gcgcggattg
   126901 gtcgtccacc ggagcaggta ctggaaatcg tgggttaaga tgtgctccat cgctagttga
   126961 agcacattgc cggatgcgac gcttgccacg tcttatccgg cctacgaata ctatggatta
   127021 acttcgcgta ggtcaggcgt tcgcgccgca tccgacaata aacaccttat ctacaacttc
   127081 agaatttctt tcacaaacgg aatggtcagc ttacgttgcg cggtaatcga cgcacgatcc
   127141 aactgatcca acgtcataaa tagcgtgcgc atttctctgt cgagccgctt cagcaagaaa
   127201 cgccccacat cttccggcag ttcaaaacca cgcaaacgcg cgcgtaactg tagcgcctgc
   127261 aacttatctt catcagaaag tggctgcaat ttgtagatct gcccccagtc gagacgcgac
   127321 gcgagatccg gtaatcccag attcaactgc cgcggtggac gatcgccggt gatcaacaac
   127381 cgtgttttgc ccgattccag aattcgattg tagagatcga aaatcgccat ctcccacaac
   127441 tcatcgcctg caatacactc aatgttgtcg atacagacca gcgacaaatg ctccataccg
   127501 tcgagcactt ccggaacaaa ccaggtgcgt ttatccagcg ggacatagcc caccgcatcg
   127561 ccacgctgcg acaattccgc gcaagccgcg tgcagcagat ggctgcgccc cgcgccttcg
   127621 cgtgcccaga gatagatgta accgctatgt tcctgacgca gcacgttttg cagcgcggcc
   127681 agtaaagagg agttatcccc cggccagaaa cttgcaaagg tttcgtcgtc aggaagataa
   127741 agtggcaaag agagctgtgc cggtgtgttc agagatacct caaccaggat ttcacaaaat
   127801 cgcgagaagt ttaccacaga atcccatgat gtttgaaccg ggcagcaaca ctgcccggtc
   127861 ggtacattat ttgtctgtta tatccgcgtc ttctgcgtcc agcaccactt cttcagggcg
   127921 cagcacactg atcagtttga aaatcaaact taagccgata ccgacgatgg tcgccagcgc
   127981 catacctttt aactccgccg cgccgatgtt taccttcgcg ccactgacgc cgatgatcaa
   128041 aatcacggaa gtcaggatca ggttctgtgc tttgttgtaa tccactttcg attcgatcaa
   128101 aacacgaata ccggaagcac caatgacacc ataaagcagc agcgaaacgc cgcccatcac
   128161 cggcaatggg atcatctgga tagcggcagc cagtttaccg acgcaggaaa gcaggatagc
   128221 gaaaatcgcc gccccgccga taacccaggt actgtaaaca cgggtgatcg ccatcacgcc
   128281 aatgttttct ccgtaagtgg tatttggcgt agagccaaag aagccggaga tcacggtcga
   128341 caggccgtta gcaaacatcg aacggtgcag acctggatcg cgcagcagat cttttttgac
   128401 gatattagcc gttactacca ggtgccctac gtgttcggca ataaccacta acgccgctgg
   128461 cagaatagtc agaatggcaa accactcgaa gcgcggcgta tagagggttg gcagtgcaaa
   128521 ccagtgagca ttaataatcg gcgtggtatc gacaattccc attgcgaaag agagcgcgta
   128581 ccccaccagc acgccaatta aaatcgggat aattgccagg aaaccacgaa acagcacgga
   128641 acctaaaacc gtgaccgcca gggtggtaat agagatgatg atggttttgg agtttggcgt
   128701 ttgcccttca gccgggagta aacccgccat accggcagct acgcccgcca gctccagacc
   128761 gatgacggca acgattgcgc ccattgccgc aggtggaaac agcacgtcca accagccggt
   128821 ccccgctttc ttcacgataa aagaaaccag gcagaacagc acgccgcaca taataaagcc
   128881 gcccagcgcg acttcatacc ctaacggcaa cagtaacaat accggtgaaa taaaggcaaa
   128941 gctggaacca agataagccg gaattttccc tttacagatg aagagataca gcagcgttcc
   129001 aataccgtta aataacagta cagtcgccgg gttaatatga aataagacgg gcaccaggac
   129061 ggttgcacca aacatggcga acaaatgttg caaactaagc gggattgtct gtaaaagtgg
   129121 cggtctttca ctcaccccga tagcacggcg cgtcatagta ttatcctctg tattatgtgt
   129181 tataggcgct ttactcaaaa aaaagccgac tcttaaagtc ggctttaatt atttttattc
   129241 tttatttcgt accaaagatt ttgtcaccgg catcgccgag gcccggaata atgtatccgt
   129301 gctcgttcag tccctggtca atcgatgcag tatacagttc gacgtccggg tgcgcttttt
   129361 ccagcgcagc gataccttct ggcgcagcga ccagcaccag aactttgatg ctgctgcagc
   129421 ccgctttttt cagcaggtcg atggtcgcga taacggaacc accggttgcc agcattgggt
   129481 caacgatcag cgccatacgc tcatcgatgt tagaaaccag tttctggaag tacggtaccg
   129541 gctccagcgt ttcttcatta cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt
   129601 tttccagcac accgtccatc ataccaagac ccgcacgcag aattggcaca acggtaattt
   129661 tcttaccttt gatctggtcg atttctaccg ggccgttcca gccttcgata gttacttttt
   129721 ccgtttcgag gtcggcggtc gcttcgtaag tcagcaggct acccacttcg gaagcgagtt
   129781 cgcgaaagcg cttggtgctg atatcttgct cacgcatcag tcccagcttg tgtttgacga
   129841 gtgggtgttt gacttccacg atcttcatac tctttctcct ttgaggggca gccacaaaaa
   129901 aatcgacgga ttatacctcc tttcttcagg gcggcaatat tattttcgtt gactttagtc
   129961 aaaatgataa cagtttgaga taaagttatt ttatattcaa atggttatga aagaagatta
   130021 ttccatccga aaactaacct ttgccctggc acaagtcttc tttcgccgcg cgcccgggga
   130081 aaagacgtgc aaaaaggttg tgtaaaacag tctcgcaaac gtttgctttc cctgttagaa
   130141 ttgcgccgaa ttttattttt ctaccgcaag taacgcgtgg ggacccaagc agtgaccgat
   130201 aaaacctctc ttagctacaa agatgccggt gttgatattg acgcgggtaa tgctctggtt
   130261 ggaagaatca aaggcgtagt gaagaaaacg cgtcgtccgg aagtgatggg cggtctgggc
   130321 ggcttcggtg cgctgtgtgc attgccgcaa aaatatcgtg aacccgtgct ggtttccggc
   130381 actgacggcg taggtaccaa gctgcgtctg gcaatggact taaaacgtca cgacaccatt
   130441 ggtattgatc tggtcgccat gtgcgttaat gacctggtgg tgcaaggtgc ggaaccgctg
   130501 tttttcctcg actattacgc aaccggaaaa ctggatgttg ataccgcttc agcggtgatc
   130561 agcggcatcg cggaaggttg tctgcaatcg ggctgttctc tggtgggtgg cgaaacggca
   130621 gaaatgccgg ggatgtatca cggtgaagat tacgatgtcg cgggtttctg cgtgggcgtg
   130681 gtagaaaaat cagaaatcat cgacggctct aaagtcagcg acggcgatgt gctgattgca
   130741 ctcggttcca gcggtccgca ctcgaacggc tattcgctgg tgcgcaaaat tcttgaagtc
   130801 agcggttgtg atccgcaaac caccgaactt gatggtaagc cattagccga tcatctgctg
   130861 gcaccgaccc gcatttacgt gaagtcagtg ctggagttga ttgaaaaggt cgatgtgcat
   130921 gccattgcgc acctgaccgg cggcggcttc tgggaaaaca ttccgcgcgt attgccagat
   130981 aatacccagg cagtgattga tgaatcttcc tggcagtggc cggaagtgtt caactggctg
   131041 caaacggcag gtaacgttga gcgccatgaa atgtatcgca ccttcaactg cggcgtcggg
   131101 atgattatcg ccctgcctgc tccggaagtg gacaaagccc tcgccctgct aaatgccaac
   131161 ggtgaaaacg cgtggaaaat cggtatcatc aaagcctctg attccgaaca acgcgtggtt
   131221 atcgaataat gaatattgtg gtgcttattt ccggcaacgg aagtaattta caggcaatta
   131281 ttgacgcctg taaaaccaac aaaattaaag gcaccgtacg ggcagttttc agcaataagg
   131341 ccgacgcgtt cggccttgaa cgcgcccgcc aggcgggtat tgcaacgcat acgctcatcg
   131401 ccagcgcgtt tgacagtcgt gaagcctatg accgggagtt gattcatgaa atcgacatgt
   131461 acgcacccga tgtggtcgtg ctggctggtt ttatgcgcat tctcagcccg gcgtttgtct
   131521 cccactatgc cgggcgtttg ctgaacattc acccttctct gctgccgaaa tatcccggat
   131581 tacacaccca tcgtcaggcg ctggaaaatg gcgatgaaga gcacggtaca tcggtgcatt
   131641 tcgtcaccga tgaactggac ggtggcccgg ttattttaca ggcgaaagtc ccggtatttg
   131701 ctggtgatac ggaagatgac atcaccgccc gcgtgcaaac ccaggaacac gctatttatc
   131761 cactggtgat tagctggttt gccgatggtc gtctgaaaat gcacgaaaac gccgcgtggc
   131821 tggatggtca acgtctgccg ccgcagggct acgctgccga cgagtaatgc ccccgtagtt
   131881 aaagcgccag ctctgccgct ggcgtttttc aattcacctg taaatcgcaa gctccagcag
   131941 tttttttccc ccttttctgg catagttgga catctgccaa tattgctcgc cataatatcc
   132001 aggcagtgtc ccgtgaataa aacggagtaa aagtggtaat gggtcaggaa aagctataca
   132061 tcgaaaaaga gctcagttgg ttatcgttca atgaacgcgt gcttcaggaa gcggcggaca
   132121 aatctaaccc gctgattgaa aggatgcgtt tcctggggat ctattccaat aaccttgatg
   132181 agttctataa agtccgcttc gctgaactga agcgacgcat cattattagc gaagaacaag
   132241 gctccaacgc tcattcccgc catttactgg gcaaaattca gtcccgggtg ctgaaagccg
   132301 atcaggaatt cgacggcctc tacaacgagc tattgctgga gatggcgcgc aaccagatct
   132361 tcctgattaa tgaacgccag ctctccgtca atcaacaaaa ctggctgcgt cattatttta
   132421 agcagtatct gcgtcagcac attacgccga ttttaatcaa tcctgacact gacttagtgc
   132481 agttcctgaa agatgattac acctatctgg cggtggaaat tatccgtagc gatagcatcc
   132541 gttacgcgct gctggagatc ccatcagata aagtgccgcg ctttgtgaat ttaccgccag
   132601 aagcgccgcg tcgacgcaag ccgatgattc ttctggataa cattctgcgt tactgccttg
   132661 atgatatttt caaaggcttc tttgattatg acgcgctgaa tgcctattca atgaagatga
   132721 cccgcgatgc cgaatacgat ttagtgcatg agatggaagc cagcctgatg gagttgatgt
   132781 cttccagtct caagcagcgt ttaactgctg agccggtgcg ttttgtttat cagcgcgata
   132841 tgcccaatgc gctggttgaa gtgttacgcg aaaaactgac tatttcccgc tacgactcca
   132901 tcgtccccgg cggtcgttat cataatttta aagactttat taatttcccc aatgtcggca
   132961 aagccaatct ggtgaacaaa ccactgccgc gtttacgcca tatttggttt gataaagccc
   133021 agttccgcaa tggttttgat gccattcgcg aacgcgatgt gttgctctat tatccttatc
   133081 acacctttga gcatgtgctg gaactgctgc gtcaggcttc gttcgacccg agcgtactgg
   133141 cgattaaaat taacatttac cgcgtggcga aagattcacg catcatcgac tcgatgatcc
   133201 acgccgcaca taacggtaag aaagtcactg tggtggttga gttacaggcg cgtttcgacg
   133261 aagaagccaa cattcactgg gcgaagcgcc tgaccgaagc aggcgtgcac gttatcttct
   133321 ctgcgccggg gctgaaaatt cacgccaaac tgttcctgat ttcacgtaaa gaaaacggtg
   133381 aagtggtccg ttacgcacac atcgggaccg ggaactttaa cgaaaaaacc gcgcgtcttt
   133441 atactgacta ttcgttgctg accgcagatg cgcgtatcac caacgaagta cggcgggtat
   133501 ttaactttat tgaaaaccca taccgcccgg tgacatttga ttatttaatg gtgtcaccgc
   133561 aaaactctcg ccgtctgtta tatgaaatgg tagaccgcga aatcgccaac gcgcagcaag
   133621 ggctgcccag tggtatcacc ctgaagctaa ataaccttgt cgataaaggc ctggttgatc
   133681 gtctgtatgc ggcctccagc tccggcgtac cggttaatct gctggttcgc ggaatgtgtt
   133741 cgctgatccc caatctggaa ggcattagcg acaacattcg tgccatcagt attgttgacc
   133801 gttaccttga acatgaccgg gtttatattt ttgaaaatgg cggcgataaa aaggtctacc
   133861 tttcttccgc cgactggatg acgcgcaata ttgattatcg tattgaagtg gcgacaccgc
   133921 tgctcgatcc gcgcctgaag cagcgggtgc tggacatcat cgacatattg ttcagcgata
   133981 cggtcaaagc acgttatatc gataaagaac tcagtaatcg ctacgttccc cgcggcaatc
   134041 gccgcaaagt acgggcgcag ttggcgattt acgactacat caaatcactc gaacaacctg
   134101 aataacccta tgccaataca cgataaatcc cctcgtccgc aggagtttgc tgcggtcgat
   134161 cttggttcaa acagttttca catggtcata gcccgtgtgg tagatggtgc catgcagatt
   134221 attggccgcc tgaaacagcg ggtgcatctg gcggacggcc tggggccaga taatatgttg
   134281 agtgaagagg caatgacgcg cggtttgaac tgtctgtcgc tgtttgccga acggctacaa
   134341 gggttttctc ctgccagcgt ctgtatagtc ggcacccata cgctgcgtca ggcgctgaac
   134401 gccactgact ttctgaaacg cgcggaaaag gtcattccct acccgattga aattatttcc
   134461 ggtaatgaag aagcccgtct gatttttatg ggcgtggaac atacccaacc ggaaaaaggt
   134521 cgcaaactgg ttattgatat tggcggcgga tctacggaac tggtgattgg tgaaaatttc
   134581 gaacctattc tcgttgaaag ccgccggatg ggttgtgtca gctttgccca gctttatttt
   134641 cctggcgggg tcatcaataa agagaatttt cagcgcgctc gcatggcggc agcacaaaaa
   134701 ctggaaactt taacctggca attccgtatt cagggctgga acgttgcaat gggcgcttcc
   134761 ggtaccataa aagccgccca tgaagtgtta atggaaatgg gcgagaaaga cgggataatt
   134821 accccggaac gtctggaaaa actggtaaaa gaaattttac gtcaccgtaa tttcgcatcg
   134881 ctgagtttac cgggtctttc cgaagagcgg aaaacagtct tcgttcctgg actggcgatt
   134941 ttatgtggtg tgtttgatgc tttagccatc cgtgaactgc gcctttctga cggggcgtta
   135001 cgcgaaggcg tactgtatga aatggaagga cgtttccgtc atcaggatgt gcgtagtcgc
   135061 accgccagca gcctcgccaa ccagtatcac atagacagcg aacaggcccg acgagtgctg
   135121 gataccacta tgcaaatgta cgaacagtgg cgggaacagc aaccgaagct ggcgcatccg
   135181 caactggagg cgctactgcg atgggccgcc atgctgcatg aggtcgggtt gaatatcaac
   135241 cacagcggtt tgcatcgcca ctccgcttat attctgcaaa acagtgactt gccgggtttt
   135301 aatcaggaac agcagctgat gatggcgaca ctggtgcgct atcaccgtaa agcgattaag
   135361 ctcgacgatc tgccgcgctt taccttgttt aagaagaaac agttcctgcc actgatacag
   135421 ctattgcgcc ttggcgtatt actaaacaat caacgtcagg caaccaccac accgccaaca
   135481 ttgacactga ttactgatga cagtcactgg acactgcgtt tcccgcatga ctggtttagt
   135541 cagaatgcgc tggtactgct tgatctggaa aaggagcaag aatactggga aggcgtggct
   135601 ggctggcggt tgaaaattga agaagaaagt accccagaaa tcgccgctta atgctttgcg
   135661 ggccgacgag aaatgtcggc ccgcattatt caggcacttt cgcgaatggg ttcgatttca
   135721 ttcagcgtat caattaacgg ttgcggctta ccaataagat aaccctgcat ataatcgatc
   135781 cccaaagaga gcaccgcctc gcggatctct tcgttttcaa cgtactctgc cactacccgc
   135841 attttcttca ttcgcgccag atggcaaatc gacgccacaa tctgataatc cagactattt
   135901 gacacaatat tgcggataaa gctgccgtca attttaagca gatcggcatt cacattttta
   135961 agccgtgcat agctggcgta gccggtgccg aaatcatcaa tcgcaatctg gcagcctaat
   136021 tcctgaagat gctgcaaggt gatttgcgcc tgcttaacat tggtcagagc attactttcg
   136081 gtgacttcaa aaataagttg ccacgcttca atctgatatt tagccagcaa ctgactgact
   136141 tcaacaggaa aacgagcctg acataccgag gttggagaca gattaatagc aaaacggtga
   136201 gcgggcatct tcgctctgtt ttcagccata aattgcagcg tatgctcaat gacccacatg
   136261 tcgatactcg acgataaacc aaattcgtgc gcgaccggca agaagctacc ggggccgatc
   136321 agttcatcat tctcaccttt catgcgcaga agaatttcat ggtaaacatc accacgcata
   136381 ccggtaatcg gctgggccat caggaaaaaa tggttgtgtt ccagcgcctg ctgtagtcga
   136441 ttcatcatcg cgactttatc tttcaattcg cgttgcaaat acattgcccc gcaacgctgc
   136501 atattttccg gggcgttggt cacgatggaa agttcggcga ccgtatttag ctctcccagc
   136561 agcaggtaga tatgattcac tggcgagcgc acatagcagt aactgacgcc aatctgcggt
   136621 tgcatcggca tcccatccca aaagaaacga aattgcttga gatgactatc cagtgcggta
   136681 atgcgctcct ggtgcgattc tgtattcagt cgcagcgcga gatcgttacc cgaaagctga
   136741 taaacatctt cacccggttc cagcaagggt gacagccagt gagaaagttt ttgctt
//
LOCUS       DAJTRW010000002        95444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRW010000002 DAJTRW010000000
VERSION     DAJTRW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO326_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO326_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5964672.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO326_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO326_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5964673.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO326_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO326_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5964674.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO326_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO326_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5964675.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO326_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO326_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5964676.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO326_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO326_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5964677.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO326_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO326_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5964678.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO326_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO326_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5964679.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO326_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO326_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964680.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO326_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO326_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5964681.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO326_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO326_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5964682.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO326_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO326_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964683.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO326_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO326_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5964684.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO326_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO326_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5964685.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO326_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO326_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5964686.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO326_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO326_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5964687.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO326_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO326_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5964688.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO326_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO326_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO326_000150"
     CDS             complement(15781..16005)
                     /locus_tag="OO326_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964689.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO326_000151"
     CDS             complement(16147..16308)
                     /locus_tag="OO326_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5964690.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO326_000152"
     CDS             complement(16318..16623)
                     /locus_tag="OO326_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964691.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO326_000153"
     CDS             complement(16620..16898)
                     /locus_tag="OO326_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964692.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO326_000154"
     CDS             complement(17187..18935)
                     /locus_tag="OO326_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5964693.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO326_000155"
     CDS             complement(18932..19231)
                     /locus_tag="OO326_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5964694.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO326_000156"
     CDS             complement(19237..19470)
                     /locus_tag="OO326_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964695.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO326_000157"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO326_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO326_000158"
     CDS             complement(19685..19906)
                     /locus_tag="OO326_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964696.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO326_000159"
     CDS             complement(19896..20096)
                     /locus_tag="OO326_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5964697.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO326_000160"
     CDS             20226..20405
                     /locus_tag="OO326_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964698.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO326_000161"
     CDS             20530..20859
                     /locus_tag="OO326_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964699.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO326_000162"
     CDS             complement(20909..21097)
                     /locus_tag="OO326_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5964700.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO326_000163"
     CDS             complement(21472..22701)
                     /locus_tag="OO326_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5964701.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OO326_000164"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OO326_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5964702.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO326_000165"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO326_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5964703.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OO326_000166"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OO326_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5964704.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OO326_000167"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OO326_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5964705.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OO326_000168"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OO326_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5964706.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OO326_000169"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OO326_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5964707.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO326_000170"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO326_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5964708.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO326_000171"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO326_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5964709.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO326_000172"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO326_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5964710.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO326_000173"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO326_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5964711.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO326_000174"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO326_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5964712.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OO326_000175"
     CDS             34739..35812
                     /locus_tag="OO326_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5964713.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OO326_000176"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OO326_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5964714.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OO326_000177"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OO326_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5964715.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OO326_000178"
     CDS             complement(40574..40786)
                     /locus_tag="OO326_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5964716.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OO326_000179"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OO326_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5964717.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OO326_000180"
     CDS             complement(41765..42304)
                     /locus_tag="OO326_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5964718.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO326_000181"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO326_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5964719.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OO326_000182"
     CDS             complement(44243..44785)
                     /locus_tag="OO326_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5964720.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO326_000183"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO326_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5964721.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO326_000184"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO326_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5964722.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO326_000185"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO326_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5964723.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OO326_000186"
     CDS             complement(47305..47682)
                     /locus_tag="OO326_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5964724.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO326_000187"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO326_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5964725.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48377..50562
                     /gene="fhuE"
                     /locus_tag="OO326_000188"
                     /pseudo
     CDS             48377..50562
                     /gene="fhuE"
                     /locus_tag="OO326_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO326_000189"
     CDS             complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO326_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5964726.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52350..53147)
                     /locus_tag="OO326_000190"
     CDS             complement(52350..53147)
                     /locus_tag="OO326_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5964727.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO326_000191"
     CDS             complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO326_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5964728.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54159..54800)
                     /locus_tag="OO326_000192"
     CDS             complement(54159..54800)
                     /locus_tag="OO326_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5964729.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO326_000193"
     CDS             complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO326_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5964730.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO326_000194"
     CDS             complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO326_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5964731.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO326_000195"
     CDS             complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO326_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5964732.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO326_000196"
     CDS             complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO326_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5964733.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO326_000197"
     CDS             complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO326_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5964734.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO326_000198"
     CDS             complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO326_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5964735.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO326_000199"
     CDS             complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO326_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5964736.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO326_000200"
     CDS             complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO326_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5964737.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO326_000201"
     CDS             complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO326_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5964738.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO326_000202"
     CDS             complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO326_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5964739.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63328..63912
                     /gene="yceF"
                     /locus_tag="OO326_000203"
     CDS             63328..63912
                     /gene="yceF"
                     /locus_tag="OO326_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5964740.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO326_000204"
     CDS             complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO326_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5964741.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65556..68747
                     /gene="rne"
                     /locus_tag="OO326_000205"
     CDS             65556..68747
                     /gene="rne"
                     /locus_tag="OO326_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5964742.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO326_000206"
                     /pseudo
     CDS             complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO326_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO326_000207"
                     /pseudo
     CDS             complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO326_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO326_000208"
     CDS             complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO326_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5964743.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO326_000209"
     CDS             complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO326_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5964744.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO326_000210"
     CDS             complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO326_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5964745.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO326_000211"
     CDS             complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO326_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5964746.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO326_000212"
                     /pseudo
     CDS             complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO326_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76098..77237)
                     /locus_tag="OO326_000213"
     CDS             complement(76098..77237)
                     /locus_tag="OO326_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5964747.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OO326_000214"
     CDS             complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OO326_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5964748.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OO326_000215"
                     /pseudo
     CDS             complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OO326_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OO326_000216"
     CDS             complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OO326_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5964749.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78947..79606
                     /gene="flgA"
                     /locus_tag="OO326_000217"
     CDS             78947..79606
                     /gene="flgA"
                     /locus_tag="OO326_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5964750.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79682..79975
                     /gene="flgM"
                     /locus_tag="OO326_000218"
     CDS             79682..79975
                     /gene="flgM"
                     /locus_tag="OO326_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5964751.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79980..80396
                     /gene="flgN"
                     /locus_tag="OO326_000219"
     CDS             79980..80396
                     /gene="flgN"
                     /locus_tag="OO326_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5964752.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OO326_000220"
     CDS             complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OO326_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5964753.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82171..83004)
                     /locus_tag="OO326_000221"
     CDS             complement(82171..83004)
                     /locus_tag="OO326_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5964754.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83006..83653)
                     /locus_tag="OO326_000222"
     CDS             complement(83006..83653)
                     /locus_tag="OO326_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5964755.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OO326_000223"
     CDS             complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OO326_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5964756.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84484..85692
                     /gene="mdtH"
                     /locus_tag="OO326_000224"
     CDS             84484..85692
                     /gene="mdtH"
                     /locus_tag="OO326_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5964757.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85756..86403
                     /gene="grxB"
                     /locus_tag="OO326_000225"
     CDS             85756..86403
                     /gene="grxB"
                     /locus_tag="OO326_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5964758.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86537..87097
                     /locus_tag="OO326_000226"
     CDS             86537..87097
                     /locus_tag="OO326_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5964759.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87203..88249
                     /gene="pyrC"
                     /locus_tag="OO326_000227"
     CDS             87203..88249
                     /gene="pyrC"
                     /locus_tag="OO326_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5964760.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88323..88568
                     /gene="dinI"
                     /locus_tag="OO326_000228"
     CDS             88323..88568
                     /gene="dinI"
                     /locus_tag="OO326_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5964761.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88858..89112
                     /gene="bssS"
                     /locus_tag="OO326_000229"
     CDS             88858..89112
                     /gene="bssS"
                     /locus_tag="OO326_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5964762.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89227..90345
                     /gene="solA"
                     /locus_tag="OO326_000230"
     CDS             89227..90345
                     /gene="solA"
                     /locus_tag="OO326_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5964763.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90393..90506
                     /locus_tag="OO326_000231"
     CDS             90393..90506
                     /locus_tag="OO326_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5964764.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90767..91333
                     /locus_tag="OO326_000232"
     CDS             90767..91333
                     /locus_tag="OO326_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5964765.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91337..91912
                     /locus_tag="OO326_000233"
     CDS             91337..91912
                     /locus_tag="OO326_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5964766.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91954..93006)
                     /locus_tag="OO326_000234"
     CDS             complement(91954..93006)
                     /locus_tag="OO326_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5964767.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93231..94151
                     /gene="lpxL"
                     /locus_tag="OO326_000235"
     CDS             93231..94151
                     /gene="lpxL"
                     /locus_tag="OO326_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5964768.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94323..94520
                     /locus_tag="OO326_000236"
     CDS             94323..94520
                     /locus_tag="OO326_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5964769.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94544..>95444
                     /locus_tag="OO326_000237"
     CDS             94544..>95444
                     /locus_tag="OO326_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5964770.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFR"
BASE COUNT        22965 a        25296 c        23548 g        23635 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gtggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc agcaaaatta
    48301 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    48361 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagtcat
    48421 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    48481 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    48541 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    48601 acatgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    48661 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    48721 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
    48781 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt
    48841 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    48901 tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag gcgatgtctc
    48961 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    49021 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    49081 ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    49141 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
    49201 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
    49261 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    49321 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    49381 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    49441 gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt ggaacagtgg
    49501 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
    49561 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt cagttcatgg
    49621 gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa
    49681 gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa atcgttatat
    49741 gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc
    49801 aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct
    49861 ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc
    49921 cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac
    49981 tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc
    50041 ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac
    50101 ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc
    50161 aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta tcttgcagag
    50221 gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc
    50281 agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat
    50341 cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac
    50401 gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac
    50461 gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc
    50521 gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag
    50581 gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac
    50641 tcatccatct cggttttcag gttatcggat ttagtaccga aaatagcctg aacaccagaa
    50701 ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac
    50761 acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct
    50821 ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca
    50881 tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg
    50941 aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag ccacagtttg
    51001 ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca
    51061 cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg
    51121 tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat accggtcagg
    51181 aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta
    51241 gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca
    51301 ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa
    51361 acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg
    51421 tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact
    51481 accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc
    51541 caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca
    51601 aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta
    51661 aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt atccgccaag
    51721 tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg
    51781 gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg
    51841 gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg
    51901 cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg
    51961 acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag cgatttaccg
    52021 accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg
    52081 tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc
    52141 taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct
    52201 atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca
    52261 atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc
    52321 cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg
    52381 atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca
    52441 cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt
    52501 cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa tcgatccagg
    52561 gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa
    52621 aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa
    52681 cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg
    52741 gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata
    52801 tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc
    52861 ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca
    52921 tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt
    52981 tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa
    53041 aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat
    53101 tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta
    53161 aagatgagga accggtaaca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag
    53221 atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg
    53281 gcaaacatcc cccagtatag cctgcaggca cgagggagaa agatggtttg ccagttcggc
    53341 gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc
    53401 atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc
    53461 cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt
    53521 ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc
    53581 gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca
    53641 agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac gactacgtaa
    53701 tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg
    53761 ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt
    53821 tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc
    53881 acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc
    53941 gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc
    54001 ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat
    54061 gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac
    54121 cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc
    54181 cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca
    54241 atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta
    54301 aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt
    54361 aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa atccccgaga
    54421 acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac
    54481 gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt
    54541 ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta
    54601 tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg
    54661 gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg
    54721 agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg
    54781 acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt
    54841 gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata
    54901 gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc
    54961 tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag
    55021 gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc
    55081 ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg
    55141 ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt
    55201 atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat
    55261 tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat acatcaaggt
    55321 gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac
    55381 ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac gcaattgctt
    55441 gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc
    55501 gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc
    55561 ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa cccgtgggcg
    55621 attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg tccctggctt
    55681 cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt
    55741 ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa
    55801 cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt
    55861 gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat taacgcatta
    55921 caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac ttcgacaagc
    55981 tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc gttaacacct
    56041 gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa attagccgca
    56101 cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc
    56161 tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga
    56221 tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc gttacgcaaa
    56281 cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg tccgctgttc
    56341 aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag cacaccattt
    56401 tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacaggaa
    56461 atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa caaactgact
    56521 ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg
    56581 cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag
    56641 agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa
    56701 aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc tttttaaaga
    56761 tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc
    56821 tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca
    56881 ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta
    56941 ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac
    57001 ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg
    57061 gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac
    57121 gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat
    57181 gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt
    57241 ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc
    57301 catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata
    57361 atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac
    57421 ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt
    57481 gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga
    57541 tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct
    57601 tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa
    57661 tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag
    57721 aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc
    57781 gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt
    57841 tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa
    57901 gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg
    57961 tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat gattctagtg
    58021 ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg
    58081 gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc
    58141 cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga
    58201 atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc
    58261 cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt
    58321 cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca
    58381 actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata
    58441 acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc
    58501 attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc
    58561 cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc
    58621 gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag
    58681 cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg
    58741 tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc
    58801 gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg
    58861 accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga
    58921 aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc
    58981 acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt
    59041 ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt
    59101 ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac
    59161 tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc
    59221 ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc
    59281 cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt
    59341 cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc
    59401 tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc
    59461 gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac
    59521 tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt
    59581 catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt ttgcgccagc
    59641 agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg
    59701 tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc
    59761 tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg tgccttccgg
    59821 tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc
    59881 aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc
    59941 cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg cagtcaacag
    60001 cgctggctgg gtttgccagg ttttattcag ttcttcagct gacccctgct gggtcagcgc
    60061 ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata
    60121 gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa
    60181 tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag agccccaagt
    60241 gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg
    60301 tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag
    60361 cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac tgataatacg
    60421 caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgc
    60481 cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc
    60541 cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg gcagcgtcag
    60601 caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg gctcttcaga
    60661 ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatctgt
    60721 tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc
    60781 agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac aggctgctgc
    60841 aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac aagctgcgct
    60901 cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc
    60961 aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc
    61021 gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga
    61081 ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc
    61141 agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact
    61201 tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt
    61261 ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa
    61321 cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg
    61381 ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact
    61441 ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata
    61501 acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca
    61561 tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg tcacaaacca
    61621 gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag
    61681 aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc tttacttctt
    61741 cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca
    61801 gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg acgttggccc
    61861 ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg
    61921 gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg
    61981 tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc agggccatac
    62041 gcattgaact cccacggctg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa
    62101 ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta
    62161 atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca
    62221 gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta
    62281 acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc
    62341 ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt
    62401 gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt
    62461 tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg
    62521 tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt
    62581 tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa
    62641 tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac
    62701 ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc
    62761 aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc
    62821 cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac
    62881 ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc
    62941 gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc
    63001 actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc
    63061 ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac
    63121 cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc
    63181 ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc
    63241 agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc
    63301 aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca
    63361 tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc acctgaggtc
    63421 gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa
    63481 aaatcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta
    63541 tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa
    63601 ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg
    63661 gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg tcacctaagc
    63721 gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt
    63781 aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg
    63841 ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg
    63901 ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat cggtgcaggc
    63961 ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt
    64021 gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc
    64081 ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag
    64141 gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc
    64201 gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg
    64261 agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac
    64321 tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct
    64381 ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc
    64441 gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga
    64501 acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc ggtcgagacg
    64561 atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc
    64621 gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat
    64681 atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga
    64741 aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc
    64801 ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac ctttacgcaa
    64861 aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat
    64921 acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt
    64981 catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttatt
    65041 tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa
    65101 tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    65161 gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac
    65221 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    65281 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    65341 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    65401 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    65461 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    65521 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    65581 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata
    65641 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    65701 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    65761 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    65821 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    65881 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    65941 tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta
    66001 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    66061 gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga
    66121 aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc
    66181 aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat atcggcgaaa
    66241 tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct gcattaggtc
    66301 gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact
    66361 accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccatccggtg
    66421 gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac tccgcacgcg
    66481 cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa gccgccgatg
    66541 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    66601 acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    66661 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    66721 gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg
    66781 gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag
    66841 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt
    66901 cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg
    66961 ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg
    67021 tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag
    67081 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac
    67141 tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa cctgccgcga
    67201 ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg
    67261 ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca
    67321 aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    67381 gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    67441 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    67501 aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca
    67561 ccgacgagca gcaagtgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    67621 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    67681 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    67741 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    67801 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    67861 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    67921 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    67981 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    68041 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    68101 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    68161 cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcgccagttg
    68221 ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag
    68281 tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga
    68341 ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa ccgatggttg
    68401 aaccgcagga agagacggca gatgcagata ttgaagaagt tgccgaaact gctgaggttg
    68461 tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag
    68521 cagaagttga aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta
    68581 ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg
    68641 actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa
    68701 cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa
    68761 gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa
    68821 aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc
    68881 cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg
    68941 agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga
    69001 tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc
    69061 aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag
    69121 cgactccagt ttgttcagct gcgtgcctaa ttccgcgcga acggtaagga cattattcag
    69181 cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc
    69241 ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag
    69301 attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa
    69361 aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat
    69421 actttctgtt ccgccaacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt
    69481 atatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    69541 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    69601 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    69661 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    69721 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    69781 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    69841 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    69901 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    69961 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    70021 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    70081 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    70141 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    70201 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    70261 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    70321 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    70381 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    70441 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    70501 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    70561 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    70621 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    70681 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    70741 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    70801 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    70861 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    70921 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    70981 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    71041 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    71101 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    71161 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    71221 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    71281 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    71341 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    71401 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    71461 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    71521 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    71581 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    71641 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    71701 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    71761 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    71821 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    71881 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    71941 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    72001 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    72061 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    72121 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    72181 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    72241 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    72301 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    72361 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    72421 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    72481 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    72541 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    72601 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    72661 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    72721 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    72781 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    72841 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    72901 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    72961 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    73021 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    73081 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    73141 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    73201 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    73261 gcccttaagc ggagtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    73321 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    73381 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    73441 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    73501 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    73561 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    73621 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    73681 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    73741 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    73801 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    73861 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    73921 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    73981 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    74041 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    74101 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    74161 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    74221 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    74281 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    74341 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    74401 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    74461 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    74521 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct
    74581 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    74641 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    74701 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    74761 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    74821 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    74881 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    74941 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    75001 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    75061 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    75121 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    75181 actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc acgcgacagt
    75241 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    75301 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    75361 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    75421 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    75481 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    75541 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    75601 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    75661 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    75721 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    75781 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    75841 cgtccagcgg gcgagaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    75901 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    75961 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    76021 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    76081 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    76141 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    76201 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagagtgcc aaagtttccc
    76261 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    76321 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    76381 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    76441 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggtt
    76501 gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa ttccagtgtt
    76561 gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac ctgccagtta
    76621 ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg actgtcgaaa
    76681 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca
    76741 gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    76801 gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    76861 ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac cttgcgtatt caacaggtta
    76921 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    76981 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    77041 gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc
    77101 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    77161 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    77221 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    77281 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct gttgccgcgg
    77341 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    77401 ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc agtcaacgaa
    77461 ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat ggtgcgcaca
    77521 accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca
    77581 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc cgcaagaaca
    77641 gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    77701 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccgctgacc
    77761 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    77821 tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    77881 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg
    77941 gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    78001 tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg
    78061 tgttaaccat ctccccgacg acatcaacgt tcggcatttt tatgtagccc tttgcatctg
    78121 ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct taacatcggc
    78181 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    78241 tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca gattactggc
    78301 cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    78361 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc
    78421 gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc
    78481 gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac ggtattgcag
    78541 ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc tgcgttgagg tcatcgtcag
    78601 tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa
    78661 atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac
    78721 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    78781 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    78841 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    78901 ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    78961 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga gcaatctcac
    79021 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    79081 ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc tttcgatgag
    79141 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    79201 atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga tgcctattgt
    79261 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc tggataccct
    79321 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    79381 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    79441 acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    79501 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg aacaggtagt
    79561 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    79621 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    79681 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    79741 caccgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    79801 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    79861 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    79921 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta actgatcgta
    79981 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    80041 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    80101 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    80161 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    80221 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    80281 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    80341 gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg atctgaatat
    80401 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    80461 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    80521 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    80581 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    80641 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    80701 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    80761 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    80821 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    80881 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    80941 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    81001 gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    81061 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    81121 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    81181 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    81241 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctcca
    81301 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    81361 tgatacacca gctgcagaat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    81421 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    81481 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    81541 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    81601 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    81661 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    81721 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    81781 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    81841 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    81901 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    81961 aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct
    82021 gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat gttacagggt
    82081 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgccta
    82141 ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat tcaatgaagt gccgcgcaca
    82201 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tattcactac
    82261 accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    82321 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aagtggtgat
    82381 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    82441 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    82501 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    82561 acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta actcaccgta
    82621 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    82681 ttccaccagc ctttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    82741 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac
    82801 aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    82861 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggctcctt gtaacgtcca
    82921 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    82981 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    83041 gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt
    83101 caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca ctgaaaagat
    83161 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    83221 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    83281 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    83341 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    83401 aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt agataatgac
    83461 gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc atgaccggtt
    83521 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    83581 ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc agggcagtaa
    83641 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    83701 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    83761 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    83821 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    83881 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    83941 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    84001 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    84061 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    84121 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    84181 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    84241 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    84301 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    84361 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatactgtcc ccgaagtgat
    84421 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    84481 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    84541 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    84601 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    84661 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    84721 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    84781 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    84841 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    84901 ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag ctggctgttg
    84961 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg
    85021 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    85081 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    85141 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    85201 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    85261 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    85321 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctacaacaa
    85381 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    85441 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    85501 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    85561 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    85621 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    85681 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga actgacagcc
    85741 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    85801 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    85861 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    85921 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    85981 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    86041 tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    86101 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    86161 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    86221 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    86281 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    86341 aattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    86401 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    86461 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    86521 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    86581 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    86641 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    86701 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    86761 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    86821 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    86881 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    86941 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    87001 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    87061 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    87121 ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca aaaacatccc
    87181 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgccc
    87241 agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac
    87301 cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg tgaccacagt
    87361 tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg ggcacgattt
    87421 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    87481 atttaatgaa gccgtgttca ccgctgcaaa actttacccg gcaaacgcaa ccactaactc
    87541 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    87601 cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca tttttgatcg
    87661 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    87721 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    87781 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    87841 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    87901 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    87961 gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    88021 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    88081 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    88141 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    88201 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    88261 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    88321 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    88381 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    88441 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    88501 aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca
    88561 gcgaataata tgcagtgatt tttgtttttg ccggatcgcc ccggcttttt tattttactt
    88621 acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat atgctaaaaa
    88681 aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa atttatctgt
    88741 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
    88801 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
    88861 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
    88921 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
    88981 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
    89041 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
    89101 cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
    89161 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
    89221 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
    89281 tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
    89341 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
    89401 aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac
    89461 gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc tgactccgca
    89521 tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga aaagctcgat
    89581 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta catcggctta
    89641 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
    89701 gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac
    89761 gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc
    89821 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa
    89881 gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt cccggcgttt
    89941 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
    90001 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
    90061 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
    90121 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
    90181 attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct gagtgggcac
    90241 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agaccaaaaa
    90301 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
    90361 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
    90421 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
    90481 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
    90541 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    90601 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
    90661 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
    90721 cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa
    90781 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
    90841 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
    90901 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
    90961 tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat
    91021 gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt tcgccatcgg
    91081 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
    91141 tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca
    91201 tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta tggccctgaa
    91261 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
    91321 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt
    91381 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
    91441 tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct ttaaagattt
    91501 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
    91561 taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga
    91621 ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
    91681 cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
    91741 gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt
    91801 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
    91861 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
    91921 tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag gaatacccag
    91981 tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
    92041 ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca
    92101 acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt ggcagccatc
    92161 atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat
    92221 ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
    92281 caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg
    92341 ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
    92401 gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
    92461 tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac
    92521 ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc
    92581 gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc
    92641 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
    92701 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
    92761 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
    92821 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
    92881 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
    92941 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
    93001 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
    93061 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
    93121 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
    93181 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
    93241 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
    93301 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
    93361 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
    93421 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
    93481 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
    93541 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
    93601 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
    93661 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
    93721 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
    93781 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg
    93841 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
    93901 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
    93961 agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct ccgccactgg
    94021 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
    94081 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
    94141 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
    94201 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
    94261 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
    94321 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
    94381 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
    94441 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
    94501 tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag
    94561 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
    94621 gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt
    94681 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
    94741 tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt
    94801 agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag
    94861 ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa
    94921 agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt
    94981 gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg
    95041 tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt
    95101 cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga gattacaggg
    95161 tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt cattgaatca
    95221 atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactgc tcagaaatcg
    95281 tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa gttcagggtt actaccccgc
    95341 aataatcagt gagtcactat tctatcgagt acagcaactg aacacagggc aatatggtag
    95401 ggcttctgta tcaagtaatc cgctatcagt caacttgttc aggg
//
LOCUS       DAJTRW010000003        75631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRW010000003 DAJTRW010000000
VERSION     DAJTRW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(437..643)
                     /locus_tag="OO326_000238"
     CDS             complement(437..643)
                     /locus_tag="OO326_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5964771.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OO326_000239"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OO326_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5964772.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="OO326_000240"
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="OO326_000240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5964773.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGVQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="OO326_000241"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="OO326_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="OO326_000242"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="OO326_000242"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5964774.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..8042
                     /gene="kdpD"
                     /locus_tag="OO326_000243"
                     /pseudo
     CDS             5362..8042
                     /gene="kdpD"
                     /locus_tag="OO326_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8039..8724
                     /gene="kdpE"
                     /locus_tag="OO326_000244"
                     /pseudo
     CDS             8039..8724
                     /gene="kdpE"
                     /locus_tag="OO326_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8999..9103
                     /locus_tag="OO326_000245"
     CDS             8999..9103
                     /locus_tag="OO326_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5964775.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9414..11611
                     /gene="speF"
                     /locus_tag="OO326_000246"
                     /pseudo
     CDS             9414..11611
                     /gene="speF"
                     /locus_tag="OO326_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11608..12927
                     /gene="potE"
                     /locus_tag="OO326_000247"
     CDS             11608..12927
                     /gene="potE"
                     /locus_tag="OO326_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5964776.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12983..14623)
                     /gene="pgm"
                     /locus_tag="OO326_000248"
     CDS             complement(12983..14623)
                     /gene="pgm"
                     /locus_tag="OO326_000248"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5964777.1"
                     /translation="MAIHNRAGQPAQLSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14649..15194)
                     /gene="seqA"
                     /locus_tag="OO326_000249"
     CDS             complement(14649..15194)
                     /gene="seqA"
                     /locus_tag="OO326_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5964778.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15379..16143
                     /gene="ybfF"
                     /locus_tag="OO326_000250"
     CDS             15379..16143
                     /gene="ybfF"
                     /locus_tag="OO326_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5964779.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16283..16576
                     /gene="ybfE"
                     /locus_tag="OO326_000251"
     CDS             16283..16576
                     /gene="ybfE"
                     /locus_tag="OO326_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5964780.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16716..17246
                     /gene="fldA"
                     /locus_tag="OO326_000252"
     CDS             16716..17246
                     /gene="fldA"
                     /locus_tag="OO326_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5964781.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17456..17542
                     /gene="uof"
                     /locus_tag="OO326_000253"
     CDS             17456..17542
                     /gene="uof"
                     /locus_tag="OO326_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5964782.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17535..17981
                     /gene="fur"
                     /locus_tag="OO326_000254"
     CDS             17535..17981
                     /gene="fur"
                     /locus_tag="OO326_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5964783.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18065..18391)
                     /locus_tag="OO326_000255"
     CDS             complement(18065..18391)
                     /locus_tag="OO326_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964784.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18428..19834)
                     /gene="chiP"
                     /locus_tag="OO326_000256"
     CDS             complement(18428..19834)
                     /gene="chiP"
                     /locus_tag="OO326_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5964785.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20413..22077)
                     /gene="glnS"
                     /locus_tag="OO326_000257"
     CDS             complement(20413..22077)
                     /gene="glnS"
                     /locus_tag="OO326_000257"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5964786.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="OO326_000258"
     CDS             complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="OO326_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5964787.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24559..25359
                     /gene="nagB"
                     /locus_tag="OO326_000259"
     CDS             24559..25359
                     /gene="nagB"
                     /locus_tag="OO326_000259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5964788.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25419..26567
                     /gene="nagA"
                     /locus_tag="OO326_000260"
     CDS             25419..26567
                     /gene="nagA"
                     /locus_tag="OO326_000260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5964789.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26576..27796
                     /gene="nagC"
                     /locus_tag="OO326_000261"
                     /gene_synonym="nagR"
     CDS             26576..27796
                     /gene="nagC"
                     /locus_tag="OO326_000261"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5964790.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27844..28596
                     /gene="nagD"
                     /locus_tag="OO326_000262"
                     /pseudo
     CDS             27844..28596
                     /gene="nagD"
                     /locus_tag="OO326_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28993..30657
                     /gene="asnB"
                     /locus_tag="OO326_000263"
     CDS             28993..30657
                     /gene="asnB"
                     /locus_tag="OO326_000263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5964791.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31036..31112
                     /locus_tag="OO326_000264"
     tRNA            31036..31112
                     /locus_tag="OO326_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31070..31072,aa:Met,seq:cat)
     gene            31121..31205
                     /locus_tag="OO326_000265"
     tRNA            31121..31205
                     /locus_tag="OO326_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31155..31157,aa:Leu,seq:tag)
     gene            31229..31303
                     /locus_tag="OO326_000266"
     tRNA            31229..31303
                     /locus_tag="OO326_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31261..31263,aa:Gln,seq:ttg)
     gene            31338..31412
                     /locus_tag="OO326_000267"
     tRNA            31338..31412
                     /locus_tag="OO326_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31370..31372,aa:Gln,seq:ttg)
     gene            31428..31504
                     /locus_tag="OO326_000268"
     tRNA            31428..31504
                     /locus_tag="OO326_000268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31462..31464,aa:Met,seq:cat)
     gene            31553..31627
                     /locus_tag="OO326_000269"
     tRNA            31553..31627
                     /locus_tag="OO326_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31585..31587,aa:Gln,seq:ctg)
     gene            31665..31739
                     /locus_tag="OO326_000270"
     tRNA            31665..31739
                     /locus_tag="OO326_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31697..31699,aa:Gln,seq:ctg)
     gene            complement(31893..33068)
                     /gene="ubiF"
                     /locus_tag="OO326_000271"
     CDS             complement(31893..33068)
                     /gene="ubiF"
                     /locus_tag="OO326_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5964792.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33214..34638
                     /gene="miaB"
                     /locus_tag="OO326_000272"
     CDS             33214..34638
                     /gene="miaB"
                     /locus_tag="OO326_000272"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5964793.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34791..35831
                     /locus_tag="OO326_000273"
     CDS             34791..35831
                     /locus_tag="OO326_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5964794.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35828..36295
                     /gene="ybeY"
                     /locus_tag="OO326_000274"
     CDS             35828..36295
                     /gene="ybeY"
                     /locus_tag="OO326_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5964795.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36385..37263
                     /gene="corC"
                     /locus_tag="OO326_000275"
     CDS             36385..37263
                     /gene="corC"
                     /locus_tag="OO326_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5964796.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37288..38826
                     /gene="lnt"
                     /locus_tag="OO326_000276"
     CDS             37288..38826
                     /gene="lnt"
                     /locus_tag="OO326_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5964797.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39223..40131
                     /gene="gltI"
                     /locus_tag="OO326_000277"
     CDS             39223..40131
                     /gene="gltI"
                     /locus_tag="OO326_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5964798.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40301..41041
                     /gene="gltJ"
                     /locus_tag="OO326_000278"
     CDS             40301..41041
                     /gene="gltJ"
                     /locus_tag="OO326_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5964799.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41041..41715
                     /gene="gltK"
                     /locus_tag="OO326_000279"
     CDS             41041..41715
                     /gene="gltK"
                     /locus_tag="OO326_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5964800.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41715..42440
                     /gene="gltL"
                     /locus_tag="OO326_000280"
     CDS             41715..42440
                     /gene="gltL"
                     /locus_tag="OO326_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5964801.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42558..43493
                     /gene="rihA"
                     /locus_tag="OO326_000281"
     CDS             42558..43493
                     /gene="rihA"
                     /locus_tag="OO326_000281"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5964802.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43577..45247
                     /gene="hscC"
                     /locus_tag="OO326_000282"
                     /pseudo
     CDS             43577..45247
                     /gene="hscC"
                     /locus_tag="OO326_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45307..46758)
                     /gene="djlC"
                     /locus_tag="OO326_000283"
     CDS             complement(45307..46758)
                     /gene="djlC"
                     /locus_tag="OO326_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5964803.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46755..47462)
                     /locus_tag="OO326_000284"
     CDS             complement(46755..47462)
                     /locus_tag="OO326_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5964804.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47564..48118
                     /locus_tag="OO326_000285"
     CDS             47564..48118
                     /locus_tag="OO326_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5964805.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48128..49555)
                     /locus_tag="OO326_000286"
     CDS             complement(48128..49555)
                     /locus_tag="OO326_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5964806.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49552..50259)
                     /locus_tag="OO326_000287"
     CDS             complement(49552..50259)
                     /locus_tag="OO326_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5964807.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50423..51400
                     /locus_tag="OO326_000288"
     CDS             50423..51400
                     /locus_tag="OO326_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5964808.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51470..51952)
                     /locus_tag="OO326_000289"
     CDS             complement(51470..51952)
                     /locus_tag="OO326_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5964809.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52187..54769
                     /gene="leuS"
                     /locus_tag="OO326_000290"
     CDS             52187..54769
                     /gene="leuS"
                     /locus_tag="OO326_000290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5964810.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54784..55365
                     /gene="lptE"
                     /locus_tag="OO326_000291"
     CDS             54784..55365
                     /gene="lptE"
                     /locus_tag="OO326_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5964811.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55365..56396
                     /gene="holA"
                     /locus_tag="OO326_000292"
     CDS             55365..56396
                     /gene="holA"
                     /locus_tag="OO326_000292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5964812.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56398..57039
                     /gene="nadD"
                     /locus_tag="OO326_000293"
     CDS             56398..57039
                     /gene="nadD"
                     /locus_tag="OO326_000293"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5964813.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57063..57674
                     /locus_tag="OO326_000294"
     CDS             57063..57674
                     /locus_tag="OO326_000294"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5964814.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57934..58251
                     /gene="rsfS"
                     /locus_tag="OO326_000295"
                     /gene_synonym="rsfA"
     CDS             57934..58251
                     /gene="rsfS"
                     /locus_tag="OO326_000295"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5964815.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58255..58722
                     /gene="rlmH"
                     /locus_tag="OO326_000296"
     CDS             58255..58722
                     /gene="rlmH"
                     /locus_tag="OO326_000296"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5964816.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58753..60654
                     /gene="mrdA"
                     /locus_tag="OO326_000297"
     CDS             58753..60654
                     /gene="mrdA"
                     /locus_tag="OO326_000297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5964817.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60657..61769
                     /gene="mrdB"
                     /locus_tag="OO326_000298"
                     /gene_synonym="rodA"
     CDS             60657..61769
                     /gene="mrdB"
                     /locus_tag="OO326_000298"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5964818.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61780..62868
                     /gene="rlpA"
                     /locus_tag="OO326_000299"
     CDS             61780..62868
                     /gene="rlpA"
                     /locus_tag="OO326_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5964819.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63007..64218
                     /gene="dacA"
                     /locus_tag="OO326_000300"
     CDS             63007..64218
                     /gene="dacA"
                     /locus_tag="OO326_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5964820.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64329..64592
                     /locus_tag="OO326_000301"
     CDS             64329..64592
                     /locus_tag="OO326_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5964821.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64693..65334
                     /gene="lipB"
                     /locus_tag="OO326_000302"
     CDS             64693..65334
                     /gene="lipB"
                     /locus_tag="OO326_000302"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5964822.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65593..66546
                     /locus_tag="OO326_000303"
     CDS             65593..66546
                     /locus_tag="OO326_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5964823.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66755..67720
                     /gene="lipA"
                     /locus_tag="OO326_000304"
     CDS             66755..67720
                     /gene="lipA"
                     /locus_tag="OO326_000304"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5964824.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67821..68024)
                     /gene="tatE"
                     /locus_tag="OO326_000305"
     CDS             complement(67821..68024)
                     /gene="tatE"
                     /locus_tag="OO326_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5964825.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68153..68941)
                     /locus_tag="OO326_000306"
     CDS             complement(68153..68941)
                     /locus_tag="OO326_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5964826.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69034..69417
                     /gene="crcB"
                     /locus_tag="OO326_000307"
     CDS             69034..69417
                     /gene="crcB"
                     /locus_tag="OO326_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5964827.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69471..69680)
                     /gene="cspE"
                     /locus_tag="OO326_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69471..69680)
                     /gene="cspE"
                     /locus_tag="OO326_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5964828.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69855..70415)
                     /gene="pagP"
                     /locus_tag="OO326_000309"
     CDS             complement(69855..70415)
                     /gene="pagP"
                     /locus_tag="OO326_000309"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5964829.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71004..72389
                     /gene="dcuC"
                     /locus_tag="OO326_000310"
     CDS             71004..72389
                     /gene="dcuC"
                     /locus_tag="OO326_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5964830.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72430..72771)
                     /locus_tag="OO326_000311"
                     /pseudo
     CDS             complement(72430..72771)
                     /locus_tag="OO326_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73109..73618)
                     /locus_tag="OO326_000312"
     CDS             complement(73109..73618)
                     /locus_tag="OO326_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5964831.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73781..74026
                     /locus_tag="OO326_000313"
     CDS             73781..74026
                     /locus_tag="OO326_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5964832.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            74001..>75631
                     /locus_tag="OO326_000314"
     CDS             74001..>75631
                     /locus_tag="OO326_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5964833.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHSTV"
BASE COUNT        18619 a        18991 c        19206 g        18815 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggctatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1621 ggtgcgcagc aaaactttct gccctatcag gcagtgaata ccgttgaagg agtgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat agacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatgc ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgttac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctac ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggctaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcgccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggctg ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaaagtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcacggcg gcgcggctga
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggtgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctcc acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggaattac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacgggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ccggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggt attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta
     8461 gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc
     8521 ctgctggcgg tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac
     8581 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     8641 ctgcgacaaa aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt
     8701 attgggtatc ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa
     8761 tttattatta aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat
     8821 ataaaacacg aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat
     8881 aattaatgat ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata
     8941 gccatttcat ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat
     9001 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttac
     9061 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
     9121 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
     9181 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
     9241 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
     9301 caaactactg atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt
     9361 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag
     9421 aattaaaaat tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg
     9481 tgaatattga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg
     9541 ttgaacgtgg aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg
     9601 caacggaaaa tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg
     9661 agcattgcga atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt
     9721 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca
     9781 atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag
     9841 ggaatcagtt cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag
     9901 acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg
     9961 cggcaaaagt gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta
    10021 acaaagtggt tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata
    10081 accacaaatc taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg
    10141 aaacggcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa
    10201 gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt
    10261 tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg
    10321 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
    10381 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
    10441 acgatccggg tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
    10501 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
    10561 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
    10621 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
    10681 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
    10741 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
    10801 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
    10861 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
    10921 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
    10981 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taacgccatc ctcttcctgc
    11041 tgactccggc agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga
    11101 aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca
    11161 tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta
    11221 tgcccgccac aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca
    11281 cgtcagcatg aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt
    11341 tcgtctaccg gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg
    11401 tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc
    11461 tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat
    11521 cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca
    11581 aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc
    11641 gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg
    11701 acaaagcttg ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc
    11761 tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt
    11821 atgggcggtt atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc
    11881 tacggcgtct cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc
    11941 accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg
    12001 ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc
    12061 attaccgtgt gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg
    12121 tttagcccga cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca
    12181 gtaggttctt ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg
    12241 aatactgatg tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg
    12301 ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat
    12361 atggagctgg caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca
    12421 gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc
    12481 tggcagttca ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa
    12541 attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt
    12601 atccagagcg ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg
    12661 ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta
    12721 gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt
    12781 gcttttgttg gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg
    12841 ctgtacggtt ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc
    12901 tttgaactga aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc
    12961 gctctttttt tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg
    13021 cttctttctc aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat
    13081 atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca
    13141 gaccgccaat agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac
    13201 ctgccagggt gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg
    13261 cggaagtcgc agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt
    13321 cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac
    13381 acataatgat gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag
    13441 cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc
    13501 atttgaaacc taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga
    13561 tcatcgcaga agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat
    13621 gctggaacag gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg
    13681 gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat
    13741 cacgcagtgc cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac
    13801 cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca
    13861 ggttgaggtt gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg
    13921 gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca
    13981 gcccttccac gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt
    14041 cgtcgaggga gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccttgt
    14101 cttccaccac tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga
    14161 taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc
    14221 cacctttttt attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat
    14281 tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga
    14341 acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt
    14401 ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc
    14461 tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat
    14521 tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat
    14581 cactcagttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct
    14641 gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac
    14701 tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa
    14761 tacggcgtgc ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt
    14821 tcatctgccg caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac
    14881 gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc
    14941 ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta
    15001 atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc
    15061 accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat
    15121 gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact
    15181 tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta
    15241 tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa
    15301 accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc
    15361 gaaaaaaaga actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac
    15421 aataattctc ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta
    15481 ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt
    15541 ctttcaccga gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc
    15601 ctggatgcac tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg
    15661 gtaatggcac ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc
    15721 gcaccggtcg actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc
    15781 agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat
    15841 gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac
    15901 gtgccggtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg
    15961 gatcaccctg ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt
    16021 gatgatttac tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac
    16081 tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat
    16141 taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg
    16201 actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt
    16261 ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct
    16321 gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca
    16381 gctgcgtatt aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt
    16441 cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa
    16501 tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag
    16561 tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc
    16621 tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc
    16681 cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg
    16741 gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag
    16801 acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca
    16861 ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact
    16921 tcttcccgac tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg
    16981 gtgaccagga agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca
    17041 ttgaaccgcg cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag
    17101 catcaaaagg tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc
    17161 agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc
    17221 atctcgacga aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat
    17281 taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtaccta
    17341 tacaatgtcc cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt
    17401 attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat
    17461 acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac
    17521 aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa
    17581 cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg
    17641 cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat
    17701 atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg
    17761 gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact
    17821 gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg
    17881 caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag
    17941 gcgattgccg cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa
    18001 gccaacctgc gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc
    18061 tcacttattt gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg
    18121 cctgatcatt tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac
    18181 tttcctggtc ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct
    18241 ggcaggcttc aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct
    18301 ctttcagacg gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg
    18361 ccatcatggc gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg
    18421 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct
    18481 ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
    18541 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
    18601 ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
    18661 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
    18721 ctggaagatt ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt
    18781 tagcgttgaa gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt
    18841 aggttggggt catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc
    18901 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    18961 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat
    19021 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    19081 caaaatattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    19141 ttttgaagtc gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt
    19201 tctgataaaa ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt
    19261 aggagaaact caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct
    19321 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa
    19381 tgtaacctgc cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc
    19441 ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    19501 cgatttcgtt cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag
    19561 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc
    19621 aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc
    19681 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    19741 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg
    19801 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    19861 tgctgcacca aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa
    19921 gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt
    19981 aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt
    20041 aagtttgcgg ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag
    20101 cggttacatt ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg
    20161 aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat
    20221 tgtttgctat ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca
    20281 aattcaatat attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag
    20341 cgtttgaaat ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg
    20401 tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt
    20461 aaataccggt ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc
    20521 aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt
    20581 gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg
    20641 cacgctgaac agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac
    20701 ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc
    20761 gtcataagta cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc
    20821 cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg
    20881 cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac
    20941 ctgacggctc cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc
    21001 gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc
    21061 attttcgttg agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc
    21121 ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg
    21181 acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc
    21241 ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga
    21301 gaattcgtac tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag
    21361 acgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc
    21421 gctgatgcaa tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg
    21481 gtgttcagca aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc
    21541 catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt
    21601 ttcgaacagc gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg
    21661 ctgcgtcagg gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc
    21721 caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga
    21781 gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc
    21841 gacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc
    21901 tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag
    21961 atagccattc ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag
    22021 atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt
    22081 ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg
    22141 acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa
    22201 aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc
    22261 gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
    22321 catggccctg agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg
    22381 ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
    22441 gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg
    22501 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga
    22561 acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt
    22621 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
    22681 gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata
    22741 ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag
    22801 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
    22861 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag
    22921 cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca
    22981 gacgggtaat acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat
    23041 agttggttgc cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga
    23101 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
    23161 tgaacaccac gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt
    23221 tctggctggc ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag
    23281 agaagccggc atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg
    23341 cgtgcaggag gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca
    23401 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
    23461 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga
    23521 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga
    23581 tgtcgccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga
    23641 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
    23701 taccggaacc cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct
    23761 gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg
    23821 cgatcggcac aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg
    23881 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
    23941 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
    24001 ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga
    24061 agattaatgc gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa
    24121 gtaaatctgg ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga
    24181 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
    24241 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg
    24301 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca
    24361 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
    24421 cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg
    24481 cctgtctgcg caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt
    24541 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
    24601 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg
    24661 tttgtactgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
    24721 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
    24781 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
    24841 gatcacgttg atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc
    24901 gatgccgagt gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt
    24961 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
    25021 tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt
    25081 gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg
    25141 gatgccgaag aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc
    25201 gccgttgaag gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa
    25261 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
    25321 ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc
    25381 cttgctcagg gcaatatttt ttaaaatcgg ggggcagaat gtatgcatta acccagggcc
    25441 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
    25501 ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
    25561 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
    25621 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
    25681 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
    25741 aacagggcgt gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc
    25801 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
    25861 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca
    25921 aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg
    25981 ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc
    26041 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
    26101 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg
    26161 atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac
    26221 tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
    26281 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
    26341 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac
    26401 tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac
    26461 gtctcggcac gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta
    26521 aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
    26581 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
    26641 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
    26701 ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
    26761 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
    26821 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct
    26881 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
    26941 gcagacgctg gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg
    27001 caagctgcgc gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag
    27061 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact
    27121 ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc
    27181 ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
    27241 cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt
    27301 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
    27361 tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt
    27421 aaagcagggc taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa
    27481 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct
    27541 gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg
    27601 tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
    27661 agcgctgaag gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc
    27721 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
    27781 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
    27841 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
    27901 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
    27961 ttgctgacca actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc
    28021 ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg
    28081 cgtcgctagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
    28141 tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca
    28201 cgttcctaca actgggacat gattcataaa gcagcctatt tcgtcgctaa cggtgcacgc
    28261 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg
    28321 tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg
    28381 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc
    28441 ggcgataacc tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg
    28501 gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg
    28561 atttacccgt cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg
    28621 gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag
    28681 ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
    28741 ccggcatgaa caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa
    28801 taaaacccgc taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt
    28861 caccaatcga caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca
    28921 gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta
    28981 acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag
    29041 ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc
    29101 ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt
    29161 aacgcggggg cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt
    29221 gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca
    29281 gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac
    29341 gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt
    29401 ggtcgcgacc atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg
    29461 tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg
    29521 gcggggagct atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg
    29581 ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg
    29641 gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt
    29701 ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa
    29761 gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg
    29821 ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac
    29881 gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc
    29941 gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag
    30001 atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc
    30061 ggttatcttt acttccataa agcgcccaac gttaaagaac tgcatgaaga gacggtgcgt
    30121 aaactgctgg ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg
    30181 ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt
    30241 aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt
    30301 tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc
    30361 gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag
    30421 caactggaaa ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat
    30481 ctgtaccgcg agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg
    30541 ggcggtcctt ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa
    30601 aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa
    30661 atgcggtact gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc
    30721 tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg
    30781 taggccagta tttcatagat taatttccca ccactattgt tgaattccga atttcatcct
    30841 gtacgacaaa agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa
    30901 tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa
    30961 aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta
    31021 tcgcgaaaaa aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    31081 ggtcacaggt tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt
    31141 agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg
    31201 caccattcac cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt
    31261 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg
    31321 gttcaccgcc cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc
    31381 attccctggt tcgaatccag gtaccccagc catcgaagaa ataatctggc tacgtagctc
    31441 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
    31501 accaaattct gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat
    31561 cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc
    31621 ccagccaatt tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc
    31681 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac
    31741 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
    31801 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
    31861 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
    31921 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
    31981 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
    32041 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
    32101 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
    32161 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
    32221 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
    32281 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
    32341 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
    32401 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
    32461 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
    32521 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
    32581 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
    32641 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
    32701 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
    32761 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
    32821 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
    32881 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
    32941 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
    33001 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
    33061 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
    33121 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
    33181 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
    33241 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
    33301 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
    33361 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
    33421 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
    33481 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
    33541 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
    33601 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
    33661 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
    33721 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
    33781 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
    33841 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
    33901 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
    33961 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
    34021 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
    34081 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
    34141 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
    34201 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
    34261 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
    34321 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
    34381 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
    34441 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
    34501 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
    34561 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
    34621 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
    34681 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
    34741 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
    34801 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
    34861 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
    34921 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
    34981 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
    35041 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
    35101 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
    35161 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
    35221 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
    35281 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
    35341 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
    35401 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
    35461 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
    35521 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
    35581 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
    35641 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
    35701 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
    35761 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
    35821 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
    35881 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
    35941 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
    36001 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
    36061 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
    36121 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
    36181 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
    36241 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
    36301 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
    36361 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
    36421 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
    36481 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
    36541 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
    36601 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
    36661 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
    36721 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
    36781 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
    36841 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
    36901 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
    36961 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
    37021 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
    37081 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
    37141 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
    37201 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
    37261 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
    37321 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
    37381 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
    37441 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
    37501 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
    37561 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
    37621 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
    37681 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
    37741 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
    37801 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
    37861 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
    37921 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
    37981 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
    38041 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
    38101 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
    38161 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
    38221 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
    38281 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
    38341 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
    38401 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
    38461 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
    38521 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
    38581 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
    38641 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
    38701 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
    38761 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
    38821 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
    38881 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
    38941 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
    39001 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
    39061 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
    39121 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
    39181 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
    39241 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
    39301 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
    39361 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
    39421 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
    39481 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
    39541 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
    39601 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
    39661 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
    39721 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
    39781 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
    39841 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
    39901 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
    39961 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
    40021 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
    40081 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
    40141 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
    40201 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
    40261 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
    40321 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
    40381 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
    40441 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
    40501 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
    40561 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
    40621 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
    40681 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
    40741 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
    40801 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
    40861 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
    40921 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
    40981 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
    41041 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
    41101 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
    41161 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
    41221 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
    41281 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
    41341 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
    41401 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
    41461 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
    41521 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
    41581 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
    41641 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
    41701 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    41761 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    41821 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    41881 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    41941 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    42001 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    42061 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    42121 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    42181 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    42241 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    42301 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    42361 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    42421 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    42481 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    42541 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    42601 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    42661 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    42721 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    42781 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    42841 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    42901 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    42961 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    43021 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    43081 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    43141 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    43201 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    43261 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    43321 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    43381 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    43441 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    43501 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    43561 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    43621 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    43681 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    43741 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    43801 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    43861 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    43921 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    43981 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    44041 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    44101 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    44161 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    44221 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    44281 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    44341 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    44401 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    44461 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    44521 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    44581 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    44641 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    44701 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    44761 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    44821 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    44881 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    44941 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    45001 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    45061 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    45121 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    45181 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    45241 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    45301 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    45361 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    45421 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    45481 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    45541 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    45601 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    45661 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    45721 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    45781 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    45841 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    45901 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    45961 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    46021 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    46081 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    46141 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    46201 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    46261 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    46321 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    46381 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    46441 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    46501 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    46561 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    46621 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    46681 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    46741 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    46801 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    46861 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    46921 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    46981 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    47041 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    47101 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    47161 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    47221 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    47281 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    47341 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    47401 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    47461 attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg atgagtataa
    47521 acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata aaaaattaat
    47581 gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac ccaagaaaac
    47641 gatgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat caccaggcag
    47701 tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact caacatcgca
    47761 atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
    47821 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
    47881 ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag caattctctg
    47941 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg gtttgatcta
    48001 cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
    48061 cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg cacattaacg
    48121 tcgactgtca cccgaagaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
    48181 agaaaataat tttgatgacg cccagacagc ccagcccgcc atcatcaacg ggcattggct
    48241 gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc tgaattaata
    48301 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg cggctcacaa
    48361 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
    48421 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
    48481 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
    48541 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agcggttttc
    48601 cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac cactaatggg taataataga
    48661 cccgatgctg acggcaccat tgcaacatta aagcatccgc ctgggtggag ggatactcgc
    48721 gccagtagta acaaagttct gccagcaggc tttcgccttc tccacaatag acgcgttgtg
    48781 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
    48841 aatccaggag ttcggggatt cccaggcggc ttgcgctata ccagcggagt aaatttctgt
    48901 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
    48961 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
    49021 gcacggcaac tggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
    49081 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
    49141 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
    49201 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
    49261 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
    49321 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
    49381 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
    49441 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
    49501 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
    49561 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
    49621 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
    49681 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
    49741 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
    49801 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
    49861 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
    49921 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
    49981 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
    50041 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttatgcgcag
    50101 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
    50161 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
    50221 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
    50281 atttaatgaa tgtaaactac atttcgaggc tcagttattt atacttttct gacgcaacat
    50341 aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa tatattttat
    50401 ctacaaccat aaggaaatga taatgatttt tacgtcaagt tgctgcgata atttatcaat
    50461 agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt
    50521 ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct tttactggct
    50581 gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc aaaagtatac
    50641 cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga aaaaagctgc
    50701 cctacaaggc catactttcg cttccaacgc ccttggctgg atactggatc gtggagaaga
    50761 ccccaactat aaagaagcgg ttacctggta tcagatagcc gcggagagcg gaatgtctta
    50821 tgcgcaaaat aatcttggat ggatgtacag aaatggcaac ggagtcgcac aagactatac
    50881 gctggcattt ttctggtaca aacaagctgc attacaaggc catagttacg cacaagacaa
    50941 tctggccgat ctttataaag acggagaagg cgttgctcaa aacaagacac tcgccgcatt
    51001 ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga
    51061 ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact ggtatctgaa
    51121 ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt ataaacacgg
    51181 acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg
    51241 caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag aaggaagacc
    51301 tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga
    51361 tgtcagtcag gaaattcgtg agactgaagc tttactgtag aaataaaaac aagggccggg
    51421 gaatttcatt ctgcccatat tctctttatt gctgtgcgaa atcagactat tacggctcaa
    51481 acgggcgtct ctggaactgg tcataaccac atttcgggca tagcgtcagc acttccggtg
    51541 tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc ccgaccactt
    51601 ctccgctgtg ataaacccca tgatgattga ggtcctggaa aatttcgcgc cattcaagct
    51661 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    51721 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    51781 cacgtctgac agctcgcgtc agctcatcga tctcggttcg cgttaactcc cctgttttta
    51841 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    51901 gttcgttcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    51961 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    52021 gcccacaaac tctgtaaatt aatgtacgaa taatccgggc atcatttcaa cgtcgagtgc
    52081 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    52141 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    52201 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    52261 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    52321 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta
    52381 ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc
    52441 tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt acgacaacat
    52501 cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    52561 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    52621 aaaaggcctg gtatataaaa agacttctgc ggtcaactgg tgcccgaacg accagaccgt
    52681 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg
    52741 taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc tgctcaacga
    52801 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    52861 tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    52921 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag ctgcgggtca
    52981 tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg
    53041 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    53101 tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    53161 cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    53221 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg
    53281 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    53341 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    53401 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgcca
    53461 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccaac
    53521 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    53581 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    53641 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    53701 cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga
    53761 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    53821 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg
    53881 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    53941 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    54001 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    54061 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    54121 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg
    54181 ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcacacag caaaaggtga
    54241 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    54301 gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    54361 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    54421 ggatcgcgct ctgatgcagg aagcactgca ggccgttgtc cgtatgctca acccgttcac
    54481 cccgcacatc tgcttcacgc tgtggcagga actcaaaggc gaaggcgata tcgacaacgc
    54541 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    54601 ggttaacggt aaagtccgtg ccaaaatcac cgtcccggtg gacgcaacgg aagaacaggt
    54661 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg
    54721 taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga
    54781 agcgtgcgat atctggcaac actgttgtta tctctggcgg tgttaatcac cgccgggtgt
    54841 ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    54901 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt
    54961 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    55021 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    55081 atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc
    55141 gccaaagtct tccgttcatt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    55201 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    55261 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    55321 actccggcaa cgcctgcacg cgtctccacc atgctgggta actgatgatt cggttgtacc
    55381 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    55441 acgatcctct gttattgcag gaaagccagg acgctgttcg ccaggtagct gcggcacaag
    55501 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    55561 cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    55621 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    55681 acgacctgct attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct
    55741 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    55801 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagaactg gatgacgcgg
    55861 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    55921 agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt gaacaggcgg
    55981 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    56041 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt
    56101 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    56161 atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    56221 tgggcgaggc gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga
    56281 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    56341 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    56401 aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatctaaaa
    56461 cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat
    56521 gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa
    56581 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    56641 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg
    56701 ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    56761 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    56821 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    56881 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    56941 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    57001 gtattgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    57061 tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca
    57121 gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata
    57181 cactgctaga tgatgtttcc tttgacttgg ttttatgcag tgaactggaa cgggcacagc
    57241 ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca
    57301 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    57361 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    57421 gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc
    57481 actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt
    57541 taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg
    57601 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    57661 tcgagaacgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    57721 cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat
    57781 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    57841 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    57901 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    57961 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc
    58021 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg
    58081 tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    58141 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    58201 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    58261 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    58321 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggcaaa
    58381 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    58441 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    58501 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtaag tctactgatt
    58561 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg
    58621 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    58681 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    58741 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    58801 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    58861 gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa
    58921 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    58981 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    59041 aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga tgacgatatt
    59101 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact
    59161 aacctaaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc
    59221 gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc
    59281 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    59341 gccaactatg cggcaacgca tgatattggt aagctgggca ttgagcgtta ctatgaagac
    59401 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    59461 cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc
    59521 aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgttacc
    59581 gaaccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    59641 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    59701 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    59761 gcggtttcgg cattaagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc
    59821 tggtggcaac taccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    59881 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    59941 tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    60001 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    60061 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggccagggt
    60121 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    60181 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    60241 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa
    60301 gacggtatgt acggtgttgc taaccgccct aatggtacgg cgaataaata ctttgccagc
    60361 gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct gaaagcgaac
    60421 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    60481 tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggcggcgcg
    60541 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    60601 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    60661 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    60721 tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    60781 atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg
    60841 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    60901 gtattatttt gctggtggcg gtagatgctt tcggagccat ctctaaaggc gctcaacgct
    60961 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    61021 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aatactggca
    61081 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    61141 catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc
    61201 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    61261 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    61321 tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    61381 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccatactg
    61441 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg
    61501 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    61561 ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    61621 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    61681 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    61741 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    61801 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    61861 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    61921 ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc
    61981 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    62041 gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac aaagctgacg
    62101 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    62161 cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg
    62221 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    62281 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc
    62341 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    62401 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    62461 gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg
    62521 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagcttcg
    62581 tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    62641 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    62701 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    62761 tggcggatcc agcttggccc attcgccaac aaagccgaag ccagtacctt gcagcaacgt
    62821 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    62881 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    62941 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    63001 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    63061 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    63121 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    63181 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    63241 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    63301 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    63361 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    63421 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    63481 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    63541 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    63601 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    63661 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    63721 gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc ttctgcgact
    63781 gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa aggccgtgaa
    63841 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    63901 aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga tcgcgcttcg
    63961 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    64021 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    64081 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    64141 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    64201 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    64261 agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    64321 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    64381 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    64441 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    64501 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    64561 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    64621 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    64681 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    64741 gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag tacccttgat
    64801 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    64861 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    64921 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    64981 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    65041 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    65101 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    65161 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    65221 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    65281 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    65341 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    65401 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    65461 gtaactcacc ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc
    65521 gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat
    65581 tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca
    65641 gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt
    65701 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
    65761 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
    65821 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
    65881 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
    65941 cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat
    66001 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
    66061 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
    66121 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcaaggga
    66181 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
    66241 ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg
    66301 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
    66361 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg
    66421 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
    66481 aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
    66541 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
    66601 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
    66661 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
    66721 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
    66781 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
    66841 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
    66901 tacacgtatc cagggcatca aagccgcaat gcgtaaaaat ggcctgcatt ctgtctgcga
    66961 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
    67021 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
    67081 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
    67141 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccaacactt
    67201 tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
    67261 gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa cgcccccaga
    67321 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac ggcctggcgc
    67381 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
    67441 gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta ttgaggtaat
    67501 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
    67561 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
    67621 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
    67681 ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata attacttaca
    67741 tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag
    67801 ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg
    67861 tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg
    67921 gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc
    67981 agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca
    68041 tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc
    68101 gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata
    68161 attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc acctgacgca
    68221 cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg
    68281 tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg
    68341 ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat
    68401 gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca gctccctgta
    68461 aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct
    68521 tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt
    68581 cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg
    68641 cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga tgaatcgtca
    68701 gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt
    68761 ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg
    68821 cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac
    68881 aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca gcaaccagca
    68941 ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta
    69001 tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt
    69061 attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt taacccgctg
    69121 catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga
    69181 atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc
    69241 accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt
    69301 ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa ccttctgggg
    69361 tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt acactaaaga
    69421 ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    69481 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    69541 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    69601 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    69661 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    69721 tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    69781 taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa gaagttacta
    69841 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    69901 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    69961 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    70021 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    70081 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    70141 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    70201 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    70261 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    70321 gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    70381 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    70441 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    70501 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    70561 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    70621 ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac gagatacaac
    70681 aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt agattatttt
    70741 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
    70801 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
    70861 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
    70921 atcgccaatt tattgggcat attttttcct taagctttag gaagttttta ttatttactt
    70981 tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    71041 ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    71101 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    71161 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    71221 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca
    71281 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    71341 ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg
    71401 ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt ccggtgatgg
    71461 taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta
    71521 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga
    71581 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    71641 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    71701 tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc
    71761 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    71821 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    71881 agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg
    71941 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    72001 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    72061 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    72121 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    72181 tgactattcc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg
    72241 tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct
    72301 cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag
    72361 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    72421 tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat
    72481 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    72541 gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    72601 cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg
    72661 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    72721 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat
    72781 tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag
    72841 atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa
    72901 agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc
    72961 atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcatcaaatg
    73021 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
    73081 gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca
    73141 acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc
    73201 gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac
    73261 ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa
    73321 gcccatattt agccatgttt agctctatct ctcggacttc attttctcct agtttattga
    73381 ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca
    73441 tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct
    73501 tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat
    73561 ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa
    73621 actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa
    73681 ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca agacgaatta
    73741 tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga
    73801 ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt
    73861 gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc
    73921 taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag
    73981 tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat
    74041 ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca
    74101 agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta
    74161 acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt
    74221 ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca ctatggagca
    74281 aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg ccctcaattc
    74341 aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg
    74401 cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt
    74461 ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg
    74521 ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac
    74581 cggaaaaacc cgccttccgc aaatgttatg agcgtctgga gctggcagcc cgcgagcatg
    74641 gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa
    74701 tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc
    74761 gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg
    74821 tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg
    74881 tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc
    74941 gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt cacatcacca
    75001 ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc
    75061 gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt gcgaaaatgc
    75121 actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt
    75181 tcggtgttgg tgggcttgag gaatacgttg ataagcatcc ggcactggct ggcgcatata
    75241 cggggccaaa tccgcaggca aaacctgata actatggcga ccacgctgtt gatgcagagc
    75301 tgtttctgaa aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa
    75361 cagaaatgtg cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa
    75421 cgattgtgcg taagccaacc gaagaacaaa aacggatgct gttactgcct gccgaggcgg
    75481 tgaacgtttc acgcaaaggc gagtttacgc ttaaagttgg cggctccctt aaaggcgcga
    75541 aaaacgttta ttacaacatg gcattaatga atgccggagt gaaaaaagtt gtggtcagat
    75601 ttgatccaca gcagttacac agcacggttt a
//
LOCUS       DAJTRW010000004        72625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRW010000004 DAJTRW010000000
VERSION     DAJTRW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO326_000315"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO326_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5964834.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO326_000316"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO326_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5964835.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO326_000317"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO326_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5964836.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO326_000318"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO326_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5964837.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO326_000319"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO326_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5964838.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO326_000320"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO326_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5964839.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO326_000321"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO326_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5964840.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO326_000322"
     CDS             complement(4598..5320)
                     /locus_tag="OO326_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5964841.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO326_000323"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO326_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5964842.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO326_000324"
     CDS             6520..6738
                     /locus_tag="OO326_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5964843.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO326_000325"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO326_000325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5964844.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO326_000326"
     CDS             complement(7472..7672)
                     /locus_tag="OO326_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5964845.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO326_000327"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO326_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO326_000328"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO326_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5964846.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO326_000329"
     CDS             10101..12014
                     /locus_tag="OO326_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5964847.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO326_000330"
     CDS             12014..13036
                     /locus_tag="OO326_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5964848.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO326_000331"
     CDS             13030..13248
                     /locus_tag="OO326_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5964849.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO326_000332"
     CDS             13302..14171
                     /locus_tag="OO326_000332"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5964850.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO326_000333"
     CDS             complement(14226..14630)
                     /locus_tag="OO326_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5964851.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO326_000334"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO326_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5964852.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO326_000335"
     CDS             15615..17705
                     /locus_tag="OO326_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5964853.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO326_000336"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO326_000336"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5964854.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO326_000337"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO326_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5964855.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO326_000338"
     CDS             complement(19673..20275)
                     /locus_tag="OO326_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5964856.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO326_000339"
     CDS             complement(20265..20432)
                     /locus_tag="OO326_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5964857.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO326_000340"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO326_000340"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5964858.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO326_000341"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO326_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5964859.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO326_000342"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO326_000342"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5964860.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO326_000343"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO326_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5964861.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO326_000344"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO326_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5964862.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO326_000345"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO326_000345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5964863.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO326_000346"
     CDS             28269..28436
                     /locus_tag="OO326_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5964864.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO326_000347"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO326_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO326_000348"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO326_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5964865.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO326_000349"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO326_000349"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5964866.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO326_000350"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO326_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5964867.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO326_000351"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO326_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5964868.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO326_000352"
     CDS             complement(33764..34849)
                     /locus_tag="OO326_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5964869.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO326_000353"
     CDS             complement(34861..36165)
                     /locus_tag="OO326_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5964870.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO326_000354"
     CDS             complement(36177..36530)
                     /locus_tag="OO326_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5964871.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO326_000355"
     CDS             complement(36541..37419)
                     /locus_tag="OO326_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5964872.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO326_000356"
     CDS             complement(37416..38642)
                     /locus_tag="OO326_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5964873.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO326_000357"
     CDS             complement(38643..39806)
                     /locus_tag="OO326_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5964874.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO326_000358"
     CDS             complement(39890..40252)
                     /locus_tag="OO326_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5964875.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO326_000359"
     CDS             complement(40269..41174)
                     /locus_tag="OO326_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964876.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO326_000360"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO326_000360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5964877.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO326_000361"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO326_000361"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5964878.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO326_000362"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO326_000362"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5964879.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO326_000363"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO326_000363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5964880.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO326_000364"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO326_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5964881.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO326_000365"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO326_000365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5964882.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO326_000366"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO326_000366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5964883.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO326_000367"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO326_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5964884.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO326_000368"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO326_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO326_000369"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO326_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5964885.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO326_000370"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO326_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5964886.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO326_000371"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO326_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5964887.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO326_000372"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO326_000372"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5964888.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO326_000373"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO326_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5964889.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO326_000374"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO326_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5964890.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO326_000375"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO326_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5964891.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO326_000376"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO326_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5964892.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO326_000377"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO326_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5964893.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO326_000378"
     CDS             complement(59604..61355)
                     /locus_tag="OO326_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5964894.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO326_000379"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO326_000379"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5964895.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO326_000380"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO326_000380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5964896.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO326_000381"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO326_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5964897.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO326_000382"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO326_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5964898.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO326_000383"
     CDS             66301..68622
                     /locus_tag="OO326_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5964899.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO326_000384"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO326_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5964900.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO326_000385"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO326_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5964901.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO326_000386"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO326_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5964902.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..>72625
                     /locus_tag="OO326_000387"
     CDS             72064..>72625
                     /locus_tag="OO326_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR5964903.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRD"
BASE COUNT        17487 a        19701 c        18363 g        17074 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccacgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgacc
//
LOCUS       DAJTRW010000005        71475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRW010000005 DAJTRW010000000
VERSION     DAJTRW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(750..2336)
                     /locus_tag="OO326_000388"
     CDS             complement(750..2336)
                     /locus_tag="OO326_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5964904.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO326_000389"
     CDS             complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO326_000389"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5964905.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4884..5288)
                     /locus_tag="OO326_000390"
     CDS             complement(4884..5288)
                     /locus_tag="OO326_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5964906.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO326_000391"
                     /pseudo
     CDS             complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO326_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO326_000392"
     CDS             complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO326_000392"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5964907.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO326_000393"
                     /pseudo
     CDS             complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO326_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9325..10095
                     /gene="glcC"
                     /locus_tag="OO326_000394"
     CDS             9325..10095
                     /gene="glcC"
                     /locus_tag="OO326_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5964908.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10219..11360)
                     /locus_tag="OO326_000395"
                     /pseudo
     CDS             complement(10219..11360)
                     /locus_tag="OO326_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11722..13452
                     /locus_tag="OO326_000396"
     CDS             11722..13452
                     /locus_tag="OO326_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5964909.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13449..14363
                     /locus_tag="OO326_000397"
     CDS             13449..14363
                     /locus_tag="OO326_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5964910.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14396..14644
                     /locus_tag="OO326_000398"
     CDS             14396..14644
                     /locus_tag="OO326_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5964911.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14644..15815
                     /locus_tag="OO326_000399"
                     /pseudo
     CDS             14644..15815
                     /locus_tag="OO326_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO326_000400"
     CDS             complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO326_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5964912.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO326_000401"
     CDS             complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO326_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5964913.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18028..18588)
                     /locus_tag="OO326_000402"
     CDS             complement(18028..18588)
                     /locus_tag="OO326_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5964914.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18600..19424)
                     /locus_tag="OO326_000403"
     CDS             complement(18600..19424)
                     /locus_tag="OO326_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964915.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19424..20773)
                     /locus_tag="OO326_000404"
     CDS             complement(19424..20773)
                     /locus_tag="OO326_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5964916.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20819..21577)
                     /locus_tag="OO326_000405"
     CDS             complement(20819..21577)
                     /locus_tag="OO326_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964917.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21609..22322)
                     /locus_tag="OO326_000406"
     CDS             complement(21609..22322)
                     /locus_tag="OO326_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5964918.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22496..23188
                     /locus_tag="OO326_000407"
     CDS             22496..23188
                     /locus_tag="OO326_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5964919.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO326_000408"
     CDS             complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO326_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5964920.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO326_000409"
     CDS             complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO326_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5964921.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27092..27958
                     /gene="yghU"
                     /locus_tag="OO326_000410"
     CDS             27092..27958
                     /gene="yghU"
                     /locus_tag="OO326_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5964922.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO326_000411"
     CDS             complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO326_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5964923.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO326_000412"
     CDS             complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO326_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5964924.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTSVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO326_000413"
     CDS             complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO326_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5964925.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29278..29772)
                     /locus_tag="OO326_000414"
     CDS             complement(29278..29772)
                     /locus_tag="OO326_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5964926.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO326_000415"
     CDS             complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO326_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5964927.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO326_000416"
     CDS             complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO326_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5964928.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO326_000417"
     CDS             complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO326_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5964929.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO326_000418"
     CDS             complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO326_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5964930.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34936..35223)
                     /locus_tag="OO326_000419"
     CDS             complement(34936..35223)
                     /locus_tag="OO326_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5964931.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35342..36229)
                     /locus_tag="OO326_000420"
     CDS             complement(35342..36229)
                     /locus_tag="OO326_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5964932.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36386..37426
                     /gene="gpr"
                     /locus_tag="OO326_000421"
     CDS             36386..37426
                     /gene="gpr"
                     /locus_tag="OO326_000421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5964933.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37466..37960)
                     /locus_tag="OO326_000422"
     CDS             complement(37466..37960)
                     /locus_tag="OO326_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5964934.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38151..39035
                     /gene="yghA"
                     /locus_tag="OO326_000423"
     CDS             38151..39035
                     /gene="yghA"
                     /locus_tag="OO326_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5964935.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO326_000424"
     CDS             complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO326_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5964936.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO326_000425"
     CDS             complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO326_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5964937.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40725..41912
                     /gene="metC"
                     /locus_tag="OO326_000426"
     CDS             40725..41912
                     /gene="metC"
                     /locus_tag="OO326_000426"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5964938.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42052..42711
                     /locus_tag="OO326_000427"
     CDS             42052..42711
                     /locus_tag="OO326_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5964939.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO326_000428"
     CDS             complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO326_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5964940.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43844..45007
                     /gene="yqhD"
                     /locus_tag="OO326_000429"
     CDS             43844..45007
                     /gene="yqhD"
                     /locus_tag="OO326_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5964941.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45112..45939
                     /gene="dkgA"
                     /locus_tag="OO326_000430"
     CDS             45112..45939
                     /gene="dkgA"
                     /locus_tag="OO326_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5964942.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46139..47065
                     /locus_tag="OO326_000431"
     CDS             46139..47065
                     /locus_tag="OO326_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5964943.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47116..47373
                     /gene="yqhH"
                     /locus_tag="OO326_000432"
     CDS             47116..47373
                     /gene="yqhH"
                     /locus_tag="OO326_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5964944.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47416..49635)
                     /locus_tag="OO326_000433"
     CDS             complement(47416..49635)
                     /locus_tag="OO326_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5964945.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO326_000434"
     CDS             complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO326_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5964946.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO326_000435"
                     /gene_synonym="parF"
     CDS             complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO326_000435"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5964947.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO326_000436"
     CDS             complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO326_000436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5964948.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55008..55490)
                     /locus_tag="OO326_000437"
     CDS             complement(55008..55490)
                     /locus_tag="OO326_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5964949.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO326_000438"
     CDS             complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO326_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5964950.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56087..56746
                     /gene="qseB"
                     /locus_tag="OO326_000439"
     CDS             56087..56746
                     /gene="qseB"
                     /locus_tag="OO326_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5964951.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56743..58017
                     /gene="qseC"
                     /locus_tag="OO326_000440"
     CDS             56743..58017
                     /gene="qseC"
                     /locus_tag="OO326_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5964952.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58063..58395)
                     /locus_tag="OO326_000441"
     CDS             complement(58063..58395)
                     /locus_tag="OO326_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5964953.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58714..59295
                     /gene="mdaB"
                     /locus_tag="OO326_000442"
     CDS             58714..59295
                     /gene="mdaB"
                     /locus_tag="OO326_000442"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5964954.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59326..59640
                     /locus_tag="OO326_000443"
     CDS             59326..59640
                     /locus_tag="OO326_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5964955.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO326_000444"
     CDS             complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO326_000444"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5964956.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO326_000445"
     CDS             complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO326_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5964957.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO326_000446"
     CDS             complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO326_000446"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5964958.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63042..63464)
                     /locus_tag="OO326_000447"
     CDS             complement(63042..63464)
                     /locus_tag="OO326_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5964959.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO326_000448"
     CDS             complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO326_000448"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5964960.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64299..65780
                     /gene="tolC"
                     /locus_tag="OO326_000449"
     CDS             64299..65780
                     /gene="tolC"
                     /locus_tag="OO326_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5964961.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65928..66599
                     /locus_tag="OO326_000450"
     CDS             65928..66599
                     /locus_tag="OO326_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5964962.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66605..67765
                     /locus_tag="OO326_000451"
     CDS             66605..67765
                     /locus_tag="OO326_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5964963.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDTGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO326_000452"
     CDS             complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO326_000452"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5964964.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68734..69507
                     /gene="zupT"
                     /locus_tag="OO326_000453"
     CDS             68734..69507
                     /gene="zupT"
                     /locus_tag="OO326_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5964965.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69565..69735)
                     /locus_tag="OO326_000454"
     CDS             complement(69565..69735)
                     /locus_tag="OO326_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5964966.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREEAAEKVE"
     gene            complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO326_000455"
     CDS             complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO326_000455"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5964967.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70753..70901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71023..71313
                     /gene="ubiK"
                     /locus_tag="OO326_000456"
     CDS             71023..71313
                     /gene="ubiK"
                     /locus_tag="OO326_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5964968.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17833 a        19053 c        17653 g        16936 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac
      841 agttcagatt ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg
      901 gagatcatct tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag
      961 acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg
     1021 tccgacccgg taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc
     1081 acgcctgtac ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg
     1141 aaggcgaatg ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt
     1201 tcggcaaaga cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc
     1261 gccgcaataa aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc
     1321 attggcgttg gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg
     1381 atctggaaat taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac
     1441 ggcttcatgg tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga
     1501 atgatttgcc ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc
     1561 accatcgcac ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag
     1621 actttaagga ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt
     1681 tccggaccaa tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc
     1741 agcgccgctg gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag
     1801 aacggcacaa gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac
     1861 ggatcgattc ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc
     1921 accgtcgcac caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt
     1981 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     2041 tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt
     2101 ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt
     2161 ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt
     2221 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     2281 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     2341 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     2401 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2461 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2521 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2581 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2641 ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt
     2701 tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc
     2761 acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc
     2821 gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc
     2881 tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata
     2941 tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc
     3001 ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc
     3061 tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc
     3121 accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg
     3181 gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta
     3241 gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc
     3301 tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct
     3361 ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc
     3421 cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc
     3481 atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc
     3541 tgagcgatac agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt
     3601 ttcagggtat tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg
     3661 aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc
     3721 gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga
     3781 ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga
     3841 cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg
     3901 gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt
     3961 ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct
     4021 tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg
     4081 acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt
     4141 tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg
     4201 ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac
     4261 tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt
     4321 tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc
     4381 tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac
     4441 gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt
     4501 gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt
     4561 tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta
     4621 taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc
     4681 tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc
     4741 tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca
     4801 tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc
     4861 attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc
     4921 tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca
     4981 acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta
     5041 cgtccgttgt tcaccatctc ttcatagccc ttagtttcac gacgccccag cgcggcggta
     5101 cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac
     5161 gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc
     5221 gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta
     5281 gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac
     5341 agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat
     5401 ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc
     5461 gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc
     5521 cgttaaggta aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc
     5581 atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt
     5641 ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg
     5701 cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc
     5761 gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca
     5821 ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc
     5881 caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag
     5941 cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag
     6001 cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc
     6061 aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt
     6121 cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg
     6181 ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt
     6241 gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc
     6301 gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata
     6361 gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc
     6421 ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc
     6481 tgccgcatct cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat
     6541 gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg
     6601 gaataaagga gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg
     6661 accgccagcg ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca
     6721 gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc
     6781 gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc
     6841 acgcaattgc tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc
     6901 ttttaccgat ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa
     6961 gccactaatg ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag
     7021 gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat
     7081 ttcagtgagc acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc
     7141 ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat
     7201 gcgcgtgccg aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag
     7261 cccgcaggcg accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg
     7321 aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg
     7381 agcggttatc accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc
     7441 cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac
     7501 cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc
     7561 acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca
     7621 tgcatggcac caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc
     7681 aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg
     7741 aactgggcgc acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg
     7801 ccgccaactt caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat
     7861 tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga
     7921 aagacgttgg caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg
     7981 ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt
     8041 cctaccgccg ggaacgcatt tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc
     8101 tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag
     8161 tcttcctgta cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg
     8221 acgggataac cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc
     8281 tccagcccgc cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt
     8341 tctaccgagt caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt
     8401 accgtcactt cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg
     8461 tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt
     8521 tgcacttcaa ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca
     8581 ccggcatttt ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc
     8641 gcgtaataga gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc
     8701 tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc
     8761 accaacaaca cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca
     8821 cgggtcacca ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc
     8881 atttgcttag gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac
     8941 tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc
     9001 agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg
     9061 tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc
     9121 gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc
     9181 ggtcagacca ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg
     9241 atgtaacata atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt
     9301 cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt
     9361 gcagagagta tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc
     9421 tcggaacgtc gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg
     9481 accgtgctgc gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca
     9541 cggcttaatc gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga
     9601 acgctgtacg atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg
     9661 gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc
     9721 gccgccagtg agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct
     9781 ttccatctcg ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca
     9841 ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca
     9901 cagcaaaaaa agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg
     9961 ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc
    10021 cacgatatcg agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac
    10081 aaggtgggaa tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac
    10141 ttaacaggat ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc
    10201 gctaaacagg atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc
    10261 cgccccaatg cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat
    10321 ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag
    10381 cgcaatcacc gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac
    10441 tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg
    10501 aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac
    10561 gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta
    10621 agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc
    10681 tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga
    10741 ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga
    10801 gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa
    10861 cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt
    10921 ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc
    10981 ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg
    11041 gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg
    11101 taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct
    11161 ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa
    11221 tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat
    11281 gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc
    11341 atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt
    11401 tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat
    11461 agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt
    11521 gtcaatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt
    11581 cataataaaa acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat
    11641 gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta
    11701 tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta
    11761 cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt
    11821 agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag
    11881 acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa
    11941 ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc
    12001 tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg
    12061 gattcctgga gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc
    12121 actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat
    12181 gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca
    12241 gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc
    12301 gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt
    12361 aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg
    12421 gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag
    12481 gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt
    12541 tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc
    12601 gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca
    12661 gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc
    12721 atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc
    12781 ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg
    12841 ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa
    12901 catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat
    12961 atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac
    13021 gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat
    13081 ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg
    13141 caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct
    13201 tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa
    13261 cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc
    13321 gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc
    13381 cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa
    13441 tccctggcat aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata
    13501 ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg
    13561 ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta
    13621 gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca
    13681 aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa
    13741 aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg
    13801 agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg
    13861 acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt
    13921 taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata
    13981 cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg
    14041 ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact
    14101 ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca
    14161 ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag
    14221 gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg
    14281 caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg
    14341 cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt
    14401 aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg
    14461 tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt
    14521 gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct
    14581 gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa
    14641 ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac
    14701 aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg
    14761 atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat
    14821 catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt
    14881 tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag
    14941 tttttcaatc gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca
    15001 tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta
    15061 tttatgacgt ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta
    15121 aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg
    15181 ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca
    15241 aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg
    15301 caacggggcg cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg
    15361 gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg
    15421 aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca
    15481 ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc
    15541 tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt
    15601 cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct
    15661 ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg
    15721 caggcatcac gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg
    15781 ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc
    15841 cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa
    15901 acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat
    15961 taaccatgat ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac
    16021 ggctacccat cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca
    16081 tcgccagggt caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc
    16141 tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag
    16201 tattccccgg cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa
    16261 gattatctaa tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat
    16321 gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa
    16381 gcgagagatc gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca
    16441 gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt
    16501 catcgtcctc ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg
    16561 gcgacgccac ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca
    16621 gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac
    16681 tgtttttcga taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa
    16741 gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat
    16801 aaccgcccgg gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca
    16861 gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga
    16921 aaacattcat cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag
    16981 gcccattagc atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt
    17041 agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag
    17101 tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag
    17161 cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc
    17221 ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg
    17281 gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc
    17341 acgcccctga tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc
    17401 gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt
    17461 agaagtataa atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag
    17521 gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag
    17581 ctccgactgc gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata
    17641 catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta
    17701 caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat
    17761 ctgccctagc gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc
    17821 aatcagtact ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc
    17881 cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac
    17941 gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc
    18001 actaacgccc tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat
    18061 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc
    18121 tttattcagc accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg
    18181 tccattgccg tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt
    18241 gccaacaaca aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg
    18301 ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt
    18361 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg
    18421 tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc
    18481 ttcctggata taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
    18541 cggggctaag agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc
    18601 tatagcggaa aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc
    18661 aacggtgcat aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
    18721 tctcccatca ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt
    18781 tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
    18841 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc
    18901 atttgctttt gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat
    18961 gaatagttca ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa
    19021 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg
    19081 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
    19141 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
    19201 tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg
    19261 agccagaatt gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt
    19321 gcaatagtcg gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca
    19381 aatagacgct cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg
    19441 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga
    19501 gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
    19561 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
    19621 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg
    19681 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
    19741 caaacaccag cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac
    19801 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
    19861 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
    19921 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
    19981 tccccaccaa cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
    20041 ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt
    20101 tgaaggcgtt atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg
    20161 tgccgccaac cagattcgac acgtaccagg caataacaaa acccacaaaa ccaaagtcaa
    20221 aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
    20281 caatcaaatc aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
    20341 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
    20401 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
    20461 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
    20521 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
    20581 tcgacttggc gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt
    20641 taccggcaca cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta
    20701 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
    20761 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt
    20821 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt
    20881 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
    20941 caatcgtcga catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc
    21001 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
    21061 ccattttttg gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
    21121 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
    21181 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
    21241 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
    21301 ttttcatgct ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa
    21361 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc
    21421 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac
    21481 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
    21541 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
    21601 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg
    21661 gctggttgcc gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta
    21721 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
    21781 tgttcatcaa tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac
    21841 agcttcaact cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    21901 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    21961 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    22021 gaaagcagat agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt
    22081 acatgtgccg ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt
    22141 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    22201 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    22261 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    22321 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    22381 gttcaacctt attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa
    22441 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
    22501 gtcaataaca cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt
    22561 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    22621 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt
    22681 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc
    22741 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
    22801 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
    22861 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
    22921 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
    22981 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
    23041 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
    23101 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
    23161 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    23221 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
    23281 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    23341 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    23401 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    23461 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    23521 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
    23581 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    23641 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc
    23701 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    23761 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    23821 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
    23881 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg
    23941 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag
    24001 gggcaatccc caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc
    24061 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
    24121 gtttacgttt gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    24181 ttttcgtccc actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    24241 cgacgatagg ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg
    24301 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    24361 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    24421 taatcgccgc cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca
    24481 gcaacaaatc gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata
    24541 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac
    24601 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    24661 agaacaacac aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa
    24721 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    24781 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
    24841 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
    24901 gtattccact ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat
    24961 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
    25021 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
    25081 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
    25141 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
    25201 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
    25261 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
    25321 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
    25381 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
    25441 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
    25501 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
    25561 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
    25621 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
    25681 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
    25741 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
    25801 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
    25861 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
    25921 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
    25981 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
    26041 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
    26101 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
    26161 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
    26221 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
    26281 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
    26341 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
    26401 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
    26461 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
    26521 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
    26581 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
    26641 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
    26701 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
    26761 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
    26821 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
    26881 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
    26941 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
    27001 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
    27061 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
    27121 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
    27181 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
    27241 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
    27301 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
    27361 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
    27421 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
    27481 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
    27541 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
    27601 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
    27661 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
    27721 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
    27781 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
    27841 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
    27901 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
    27961 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
    28021 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
    28081 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
    28141 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
    28201 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
    28261 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
    28321 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
    28381 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
    28441 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
    28501 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
    28561 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
    28621 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
    28681 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggaggtgacg cgcttaacat
    28741 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
    28801 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
    28861 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
    28921 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
    28981 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
    29041 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
    29101 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
    29161 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
    29221 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
    29281 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
    29341 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
    29401 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
    29461 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
    29521 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
    29581 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    29641 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    29701 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    29761 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    29821 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    29881 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    29941 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
    30001 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    30061 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
    30121 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
    30181 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
    30241 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    30301 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    30361 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    30421 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
    30481 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    30541 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
    30601 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    30661 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
    30721 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
    30781 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
    30841 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    30901 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    30961 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    31021 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    31081 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    31141 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    31201 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    31261 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    31321 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    31381 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    31441 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    31501 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    31561 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    31621 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
    31681 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
    31741 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    31801 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    31861 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
    31921 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
    31981 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    32041 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
    32101 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
    32161 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
    32221 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    32281 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    32341 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    32401 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    32461 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    32521 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    32581 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    32641 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    32701 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    32761 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    32821 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    32881 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    32941 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    33001 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    33061 ccgcctttat cgagacgttc cactcctttc tggttgcaca gctcgcactt atgcagcgca
    33121 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    33181 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    33241 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    33301 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    33361 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    33421 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    33481 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    33541 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    33601 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    33661 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    33721 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    33781 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    33841 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
    33901 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    33961 agccgaggtg gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa
    34021 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    34081 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    34141 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    34201 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
    34261 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
    34321 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
    34381 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
    34441 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
    34501 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    34561 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    34621 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    34681 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    34741 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
    34801 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    34861 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    34921 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    34981 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    35041 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    35101 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    35161 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    35221 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    35281 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    35341 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    35401 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    35461 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    35521 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    35581 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    35641 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    35701 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    35761 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    35821 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    35881 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    35941 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    36001 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    36061 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    36121 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    36181 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    36241 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    36301 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    36361 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    36421 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    36481 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    36541 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    36601 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    36661 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    36721 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    36781 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    36841 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    36901 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    36961 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    37021 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    37081 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    37141 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    37201 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    37261 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    37321 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    37381 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    37441 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    37501 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    37561 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    37621 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    37681 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    37741 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    37801 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    37861 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    37921 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    37981 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    38041 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    38101 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    38161 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    38221 caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    38281 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    38341 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    38401 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    38461 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    38521 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    38581 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    38641 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    38701 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    38761 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    38821 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    38881 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    38941 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    39001 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    39061 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    39121 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    39181 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    39241 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    39301 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    39361 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    39421 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    39481 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    39541 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    39601 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    39661 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    39721 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    39781 agcggcgctg gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac
    39841 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    39901 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    39961 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    40021 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    40081 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    40141 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    40201 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    40261 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    40321 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    40381 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    40441 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    40501 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    40561 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    40621 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    40681 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    40741 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    40801 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    40861 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    40921 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    40981 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    41041 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    41101 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    41161 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    41221 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    41281 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    41341 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    41401 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    41461 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    41521 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    41581 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    41641 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    41701 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    41761 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    41821 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    41881 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    41941 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    42001 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    42061 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    42121 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    42181 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    42241 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    42301 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    42361 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    42421 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    42481 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    42541 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    42601 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    42661 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    42721 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    42781 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    42841 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    42901 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    42961 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    43021 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    43081 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    43141 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    43201 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    43261 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    43321 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    43381 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    43441 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    43501 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    43561 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    43621 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    43681 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    43741 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    43801 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    43861 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    43921 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    43981 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    44041 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    44101 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    44161 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    44221 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    44281 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    44341 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    44401 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    44461 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    44521 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    44581 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    44641 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    44701 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    44761 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    44821 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    44881 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    44941 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    45001 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    45061 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    45121 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    45181 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    45241 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    45301 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    45361 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    45421 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    45481 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    45541 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    45601 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    45661 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    45721 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    45781 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    45841 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    45901 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    45961 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    46021 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    46081 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    46141 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    46201 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    46261 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    46321 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    46381 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    46441 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    46501 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    46561 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    46621 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    46681 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    46741 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    46801 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    46861 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    46921 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    46981 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    47041 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    47101 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    47161 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    47221 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    47281 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    47341 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    47401 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    47461 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    47521 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    47581 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    47641 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    47701 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    47761 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    47821 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    47881 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    47941 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    48001 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    48061 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    48121 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    48181 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    48241 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    48301 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    48361 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    48421 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    48481 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    48541 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    48601 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    48661 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    48721 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    48781 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    48841 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    48901 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    48961 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    49021 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    49081 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    49141 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    49201 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    49261 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    49321 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    49381 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    49441 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    49501 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    49561 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    49621 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    49681 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    49741 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    49801 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    49861 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    49921 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    49981 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    50041 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    50101 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    50161 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    50221 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    50281 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    50341 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    50401 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    50461 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    50521 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    50581 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    50641 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    50701 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    50761 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    50821 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    50881 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    50941 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    51001 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    51061 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    51121 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    51181 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    51241 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    51301 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    51361 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    51421 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    51481 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    51541 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    51601 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    51661 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    51721 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    51781 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    51841 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    51901 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    51961 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    52021 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    52081 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    52141 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    52201 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    52261 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    52321 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    52381 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    52441 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    52501 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    52561 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    52621 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    52681 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    52741 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    52801 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    52861 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    52921 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    52981 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    53041 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    53101 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    53161 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    53221 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    53281 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    53341 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    53401 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    53461 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    53521 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    53581 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    53641 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    53701 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    53761 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    53821 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    53881 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    53941 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    54001 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    54061 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    54121 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    54181 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg
    54241 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    54301 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    54361 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    54421 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    54481 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    54541 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    54601 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    54661 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    54721 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    54781 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    54841 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    54901 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    54961 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    55021 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    55081 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    55141 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    55201 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg
    55261 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    55321 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    55381 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    55441 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    55501 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    55561 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    55621 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    55681 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    55741 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    55801 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    55861 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    55921 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    55981 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    56041 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    56101 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    56161 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    56221 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    56281 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    56341 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    56401 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    56461 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    56521 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    56581 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    56641 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    56701 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    56761 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    56821 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    56881 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    56941 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    57001 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    57061 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    57121 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    57181 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    57241 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    57301 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    57361 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    57421 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    57481 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    57541 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    57601 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    57661 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    57721 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    57781 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    57841 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    57901 gctaccggta gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    57961 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    58021 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    58081 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    58141 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    58201 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    58261 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    58321 agaatctatt aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat
    58381 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    58441 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    58501 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    58561 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    58621 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    58681 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    58741 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    58801 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    58861 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    58921 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    58981 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    59041 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    59101 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    59161 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    59221 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    59281 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    59341 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    59401 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    59461 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    59521 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    59581 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    59641 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    59701 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    59761 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    59821 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    59881 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    59941 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    60001 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    60061 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    60121 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    60181 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    60241 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    60301 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    60361 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    60421 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    60481 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    60541 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    60601 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    60661 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    60721 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    60781 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    60841 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    60901 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    60961 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    61021 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    61081 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    61141 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    61201 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    61261 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    61321 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    61381 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    61441 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    61501 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    61561 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    61621 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    61681 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    61741 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    61801 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    61861 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    61921 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    61981 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    62041 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    62101 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    62161 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    62221 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    62281 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    62341 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    62401 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    62461 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    62521 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    62581 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    62641 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    62701 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    62761 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    62821 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    62881 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    62941 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    63001 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    63061 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    63121 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    63181 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    63241 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    63301 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    63361 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    63421 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    63481 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    63541 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    63601 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    63661 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    63721 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    63781 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    63841 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    63901 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    63961 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    64021 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    64081 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    64141 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    64201 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    64261 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    64321 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    64381 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    64441 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    64501 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    64561 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    64621 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    64681 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    64741 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    64801 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    64861 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    64921 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    64981 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    65041 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    65101 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    65161 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    65221 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    65281 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    65341 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    65401 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    65461 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    65521 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    65581 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    65641 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    65701 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    65761 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    65821 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    65881 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    65941 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    66001 ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    66061 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    66121 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    66181 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    66241 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    66301 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    66361 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    66421 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    66481 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    66541 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    66601 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    66661 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    66721 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    66781 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    66841 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    66901 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    66961 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    67021 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    67081 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    67141 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    67201 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    67261 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    67321 gctgcgcgaa atgttctcca ccaagctgga agatactggc gtacgctggc tggaaccggc
    67381 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    67441 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    67501 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    67561 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    67621 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    67681 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    67741 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    67801 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    67861 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    67921 tctggcgttg ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    67981 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    68041 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    68101 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    68161 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    68221 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    68281 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    68341 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    68401 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    68461 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    68521 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    68581 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    68641 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    68701 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    68761 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    68821 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    68881 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    68941 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    69001 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    69061 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    69121 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    69181 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    69241 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    69301 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    69361 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    69421 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    69481 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    69541 acttctgcca gcttcacgaa agccttattc taccttttcg gctgcctctt ctctcgtact
    69601 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    69661 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    69721 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    69781 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    69841 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    69901 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    69961 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    70021 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    70081 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    70141 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    70201 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    70261 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    70321 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    70381 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    70441 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    70501 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    70561 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    70621 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac
    70681 cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat
    70741 gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac
    70801 cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat
    70861 ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa
    70921 tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc
    70981 actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa
    71041 attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg
    71101 gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt
    71161 gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg
    71221 gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag
    71281 ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt
    71341 atgaattaat cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga
    71401 gtatcatcac aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt
    71461 tttacttaat cgata
//
LOCUS       DAJTRW010000006        67662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRW010000006 DAJTRW010000000
VERSION     DAJTRW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..228
                     /locus_tag="OO326_000457"
     CDS             <1..228
                     /locus_tag="OO326_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5964969.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO326_000458"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO326_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO326_000459"
     CDS             1365..1688
                     /locus_tag="OO326_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5964970.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO326_000460"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO326_000460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5964971.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO326_000461"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO326_000461"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5964972.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO326_000462"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO326_000462"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5964973.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO326_000463"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO326_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5964974.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO326_000464"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO326_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5964975.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO326_000465"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO326_000465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5964976.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO326_000466"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO326_000466"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5964977.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO326_000467"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO326_000467"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5964978.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO326_000468"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO326_000468"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5964979.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO326_000469"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO326_000469"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5964980.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO326_000470"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO326_000470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5964981.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO326_000471"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO326_000471"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5964982.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO326_000472"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO326_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5964983.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO326_000473"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO326_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5964984.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO326_000474"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO326_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5964985.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO326_000475"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO326_000475"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5964986.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO326_000476"
     CDS             complement(21226..23058)
                     /locus_tag="OO326_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5964987.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO326_000477"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO326_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5964988.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO326_000478"
     CDS             complement(23806..24300)
                     /locus_tag="OO326_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5964989.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO326_000479"
     CDS             complement(24383..24838)
                     /locus_tag="OO326_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5964990.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO326_000480"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO326_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5964991.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO326_000481"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO326_000481"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5964992.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO326_000482"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO326_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5964993.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO326_000483"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO326_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5964994.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO326_000484"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO326_000484"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5964995.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO326_000485"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO326_000485"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5964996.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO326_000486"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO326_000486"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5964997.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO326_000487"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO326_000487"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5964998.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO326_000488"
     CDS             33414..34307
                     /locus_tag="OO326_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5964999.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO326_000489"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO326_000489"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5965000.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHKSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO326_000490"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO326_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5965001.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO326_000491"
     CDS             complement(36223..36939)
                     /locus_tag="OO326_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5965002.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO326_000492"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO326_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5965003.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO326_000493"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO326_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5965004.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO326_000494"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO326_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5965005.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO326_000495"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO326_000495"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5965006.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO326_000496"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO326_000496"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5965007.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO326_000497"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO326_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5965008.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO326_000498"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO326_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5965009.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO326_000499"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO326_000499"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5965010.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO326_000500"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO326_000500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5965011.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO326_000501"
     CDS             complement(45788..46447)
                     /locus_tag="OO326_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5965012.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO326_000502"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO326_000502"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5965013.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO326_000503"
     CDS             complement(47342..48355)
                     /locus_tag="OO326_000503"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5965014.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO326_000504"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO326_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5965015.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO326_000505"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO326_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5965016.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO326_000506"
     CDS             complement(50648..51826)
                     /locus_tag="OO326_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5965017.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO326_000507"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO326_000507"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5965018.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO326_000508"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO326_000508"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5965019.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO326_000509"
     CDS             complement(55608..55886)
                     /locus_tag="OO326_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5965020.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO326_000510"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO326_000510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5965021.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO326_000511"
     CDS             complement(56468..57277)
                     /locus_tag="OO326_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5965022.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO326_000512"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO326_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5965023.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO326_000513"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO326_000513"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5965024.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO326_000514"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO326_000514"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5965025.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO326_000515"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO326_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5965026.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO326_000516"
     CDS             complement(60978..61868)
                     /locus_tag="OO326_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5965027.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO326_000517"
     CDS             complement(61855..62379)
                     /locus_tag="OO326_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5965028.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO326_000518"
     CDS             complement(62376..62846)
                     /locus_tag="OO326_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5965029.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO326_000519"
     CDS             complement(62843..63391)
                     /locus_tag="OO326_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5965030.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO326_000520"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO326_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO326_000521"
     CDS             complement(64138..64860)
                     /locus_tag="OO326_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5965031.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO326_000522"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO326_000522"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO326_000523"
     CDS             complement(66023..66778)
                     /locus_tag="OO326_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5965032.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO326_000524"
     CDS             complement(66860..67423)
                     /locus_tag="OO326_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5965033.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16918 a        18281 c        16784 g        15679 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaggatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgtttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt acttttgtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt ta
//
LOCUS       DAJTRW010000007        66877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRW010000007 DAJTRW010000000
VERSION     DAJTRW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO326_000525"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO326_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5965034.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO326_000526"
     CDS             complement(1151..1237)
                     /locus_tag="OO326_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5965035.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO326_000527"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO326_000527"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5965036.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO326_000528"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO326_000528"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5965037.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO326_000529"
     CDS             3586..3630
                     /locus_tag="OO326_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965038.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO326_000530"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO326_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5965039.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO326_000531"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO326_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5965040.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO326_000532"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO326_000532"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5965041.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO326_000533"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO326_000533"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5965042.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO326_000534"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO326_000534"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5965043.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO326_000535"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO326_000535"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5965044.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO326_000536"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO326_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5965045.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO326_000537"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO326_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5965046.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO326_000538"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO326_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5965047.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO326_000539"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO326_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5965048.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO326_000540"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO326_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5965049.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO326_000541"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO326_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5965050.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO326_000542"
     CDS             complement(16981..17745)
                     /locus_tag="OO326_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5965051.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO326_000543"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO326_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5965052.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHLPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO326_000544"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO326_000544"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5965053.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO326_000545"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO326_000545"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5965054.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO326_000546"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO326_000546"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5965055.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO326_000547"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO326_000547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5965056.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO326_000548"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO326_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5965057.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO326_000549"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO326_000549"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5965058.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO326_000550"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO326_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5965059.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO326_000551"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO326_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5965060.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO326_000552"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO326_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5965061.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO326_000553"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO326_000553"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5965062.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO326_000554"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO326_000554"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5965063.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO326_000555"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO326_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5965064.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO326_000556"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO326_000556"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5965065.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO326_000557"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO326_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5965066.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO326_000558"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO326_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5965067.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO326_000559"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO326_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5965068.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO326_000560"
     CDS             complement(40421..41815)
                     /locus_tag="OO326_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5965069.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO326_000561"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO326_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5965070.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO326_000562"
     CDS             complement(42158..43444)
                     /locus_tag="OO326_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5965071.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO326_000563"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO326_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO326_000564"
     CDS             complement(44451..45221)
                     /locus_tag="OO326_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5965072.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO326_000565"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO326_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5965073.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO326_000566"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO326_000566"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5965074.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO326_000567"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO326_000567"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5965075.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO326_000568"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO326_000568"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5965076.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO326_000569"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO326_000569"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5965077.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO326_000570"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO326_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5965078.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO326_000571"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO326_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5965079.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO326_000572"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO326_000572"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5965080.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO326_000573"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO326_000573"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5965081.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO326_000574"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO326_000574"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5965082.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO326_000575"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO326_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5965083.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO326_000576"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO326_000576"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5965084.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO326_000577"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO326_000577"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5965085.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO326_000578"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO326_000578"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5965086.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO326_000579"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO326_000579"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5965087.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO326_000580"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO326_000580"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5965088.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="OO326_000581"
     CDS             complement(66071..66289)
                     /locus_tag="OO326_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5965089.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="OO326_000582"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="OO326_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5965090.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16210 a        18220 c        17235 g        15212 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggca ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaaga
//
LOCUS       DAJTRW010000008        66642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRW010000008 DAJTRW010000000
VERSION     DAJTRW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO326_000583"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO326_000583"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5965091.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO326_000584"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO326_000584"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5965092.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO326_000585"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO326_000585"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5965093.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO326_000586"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO326_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5965094.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO326_000587"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO326_000587"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5965095.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO326_000588"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO326_000588"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5965096.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO326_000589"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO326_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5965097.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO326_000590"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO326_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5965098.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO326_000591"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO326_000591"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5965099.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO326_000592"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO326_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5965100.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO326_000593"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO326_000593"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5965101.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO326_000594"
     CDS             complement(15665..16702)
                     /locus_tag="OO326_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5965102.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO326_000595"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO326_000595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5965103.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO326_000596"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO326_000596"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5965104.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO326_000597"
     CDS             19444..19884
                     /locus_tag="OO326_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5965105.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO326_000598"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO326_000598"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5965106.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO326_000599"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO326_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO326_000600"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO326_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5965107.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO326_000601"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO326_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5965108.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO326_000602"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO326_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5965109.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO326_000603"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO326_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5965110.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO326_000604"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO326_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5965111.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO326_000605"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO326_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5965112.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO326_000606"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO326_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5965113.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO326_000607"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO326_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5965114.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO326_000608"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO326_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5965115.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO326_000609"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO326_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5965116.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO326_000610"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO326_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5965117.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO326_000611"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO326_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5965118.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO326_000612"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO326_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5965119.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO326_000613"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO326_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5965120.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OO326_000614"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OO326_000614"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5965121.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OO326_000615"
     CDS             37412..37681
                     /locus_tag="OO326_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5965122.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OO326_000616"
     CDS             complement(37684..38043)
                     /locus_tag="OO326_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5965123.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OO326_000617"
     CDS             38184..38810
                     /locus_tag="OO326_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5965124.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OO326_000618"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OO326_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5965125.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO326_000619"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO326_000619"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5965126.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OO326_000620"
     CDS             41650..42315
                     /locus_tag="OO326_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5965127.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OO326_000621"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OO326_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5965128.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OO326_000622"
     CDS             complement(42949..44166)
                     /locus_tag="OO326_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5965129.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OO326_000623"
     CDS             44300..45349
                     /locus_tag="OO326_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5965130.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OO326_000624"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OO326_000624"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5965131.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OO326_000625"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OO326_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5965132.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OO326_000626"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OO326_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5965133.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OO326_000627"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OO326_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5965134.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OO326_000628"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OO326_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5965135.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OO326_000629"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OO326_000629"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5965136.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OO326_000630"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OO326_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5965137.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OO326_000631"
     CDS             complement(51544..51903)
                     /locus_tag="OO326_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5965138.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OO326_000632"
     CDS             complement(51900..52175)
                     /locus_tag="OO326_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5965139.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OO326_000633"
     CDS             complement(52234..53358)
                     /locus_tag="OO326_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5965140.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO326_000634"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO326_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5965141.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OO326_000635"
     CDS             complement(56090..57157)
                     /locus_tag="OO326_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5965142.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OO326_000636"
     CDS             complement(57523..59145)
                     /locus_tag="OO326_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5965143.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="OO326_000637"
     CDS             complement(59407..60996)
                     /locus_tag="OO326_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5965144.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="OO326_000638"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="OO326_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="OO326_000639"
     CDS             complement(62499..62975)
                     /locus_tag="OO326_000639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965145.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="OO326_000640"
     CDS             complement(62968..63732)
                     /locus_tag="OO326_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5965146.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="OO326_000641"
                     /pseudo
     CDS             63978..64739
                     /locus_tag="OO326_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64846..65346)
                     /locus_tag="OO326_000642"
     CDS             complement(64846..65346)
                     /locus_tag="OO326_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965147.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65497..>66642)
                     /locus_tag="OO326_000643"
     CDS             complement(65497..>66642)
                     /locus_tag="OO326_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5965148.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16190 a        17888 c        16346 g        16218 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacta
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctgacgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctaaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggcctgaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg atgccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaacatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggaaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcataa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgatgcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaatcccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gccttccgca ctcaagccag aaacataagc
    65641 aataccatct tcacctacaa tgccggccat attttcagtg tctttgctat aactaatcac
    65701 agcgccgaag gggatgggtt taccctcttt ctgttttaag gttactaatc ctttatcccc
    65761 gctcttcact gccaatgtcg caggcacaac ggctccagct gtcggtacaa ccaattgact
    65821 agtttgtggt aagtcgtaat tcttacctat cgttttttca tccagaataa catcgtctct
    65881 tcggtatggc tgcactggca gtattgcata cccacgaaaa tcagtactca gtcccggcca
    65941 gccattaacg ttaacccccg ttgctccggg tgcctctata agggctaatg tcccatccac
    66001 attttgacca aatgtaagtc catcccttgt aataagtatc ccaccgctta aatttgcacc
    66061 tagctgtttc atgttttcgc tggagctgta atggctacta aactgaccat atgatccatt
    66121 tttgccaact tgcacaatgt tcatcgtgct cttcggttgt gactgatgag cgttagttat
    66181 atttgtcgct acatcccacc atacaccacc actattctga cctgataatg aaacctgata
    66241 gttattaccc tgctctcccg aacgactcat ctgtgaagac gcataaattt gattttcgtc
    66301 acccagcgat aatggcactg aaatacccac gcttaatttc cgatctactt tccatttatg
    66361 cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat gttatgcctc tccataccgt
    66421 actataactc aggctggtag accgcgtgtt tcctgtgccc cgggaagaat tatacctcgt
    66481 ggtattgaag ttcaaattcc cgatattacc aaggggctgg gataaggata tcgtagagcc
    66541 atcgtgacta ttcttatctc tttttctgtt ccgttcatcc tgtgcactta ctgcttcagt
    66601 cagtgtggca taacctgatg cataacggtt cgtggttata ga
//
LOCUS       DAJTRW010000009        66021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRW010000009 DAJTRW010000000
VERSION     DAJTRW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(119..598)
                     /gene="folK"
                     /locus_tag="OO326_000644"
     CDS             complement(119..598)
                     /gene="folK"
                     /locus_tag="OO326_000644"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5965149.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(595..2013)
                     /gene="pcnB"
                     /locus_tag="OO326_000645"
     CDS             complement(595..2013)
                     /gene="pcnB"
                     /locus_tag="OO326_000645"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5965150.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2052..2978)
                     /gene="gluQRS"
                     /locus_tag="OO326_000646"
     CDS             complement(2052..2978)
                     /gene="gluQRS"
                     /locus_tag="OO326_000646"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5965151.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3015..3470)
                     /gene="dksA"
                     /locus_tag="OO326_000647"
     CDS             complement(3015..3470)
                     /gene="dksA"
                     /locus_tag="OO326_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5965152.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3648..4352)
                     /gene="sfsA"
                     /locus_tag="OO326_000648"
     CDS             complement(3648..4352)
                     /gene="sfsA"
                     /locus_tag="OO326_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5965153.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4367..4897)
                     /gene="thpR"
                     /locus_tag="OO326_000649"
     CDS             complement(4367..4897)
                     /gene="thpR"
                     /locus_tag="OO326_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5965154.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4971..7400
                     /gene="hrpB"
                     /locus_tag="OO326_000650"
     CDS             4971..7400
                     /gene="hrpB"
                     /locus_tag="OO326_000650"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5965155.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7596..10130
                     /gene="mrcB"
                     /locus_tag="OO326_000651"
     CDS             7596..10130
                     /gene="mrcB"
                     /locus_tag="OO326_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5965156.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10350..12593
                     /gene="fhuA"
                     /locus_tag="OO326_000652"
     CDS             10350..12593
                     /gene="fhuA"
                     /locus_tag="OO326_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5965157.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12644..13441
                     /gene="fhuC"
                     /locus_tag="OO326_000653"
     CDS             12644..13441
                     /gene="fhuC"
                     /locus_tag="OO326_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5965158.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13441..14331
                     /gene="fhuD"
                     /locus_tag="OO326_000654"
     CDS             13441..14331
                     /gene="fhuD"
                     /locus_tag="OO326_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5965159.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14328..16310
                     /gene="fhuB"
                     /locus_tag="OO326_000655"
     CDS             14328..16310
                     /gene="fhuB"
                     /locus_tag="OO326_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5965160.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16345..17625)
                     /gene="hemL"
                     /locus_tag="OO326_000656"
     CDS             complement(16345..17625)
                     /gene="hemL"
                     /locus_tag="OO326_000656"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5965161.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAVAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17850..19271
                     /gene="clcA"
                     /locus_tag="OO326_000657"
     CDS             17850..19271
                     /gene="clcA"
                     /locus_tag="OO326_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5965162.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19295..19360
                     /locus_tag="OO326_000658"
     CDS             19295..19360
                     /locus_tag="OO326_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965163.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19353..19697
                     /gene="erpA"
                     /locus_tag="OO326_000659"
     CDS             19353..19697
                     /gene="erpA"
                     /locus_tag="OO326_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5965164.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19744..20367)
                     /locus_tag="OO326_000660"
     CDS             complement(19744..20367)
                     /locus_tag="OO326_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5965165.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20405..21205)
                     /gene="btuF"
                     /locus_tag="OO326_000661"
     CDS             complement(20405..21205)
                     /gene="btuF"
                     /locus_tag="OO326_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5965166.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21198..21896)
                     /gene="mtnN"
                     /locus_tag="OO326_000662"
     CDS             complement(21198..21896)
                     /gene="mtnN"
                     /locus_tag="OO326_000662"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5965167.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21980..23497
                     /gene="dgt"
                     /locus_tag="OO326_000663"
     CDS             21980..23497
                     /gene="dgt"
                     /locus_tag="OO326_000663"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5965168.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23627..25051
                     /gene="degP"
                     /locus_tag="OO326_000664"
     CDS             23627..25051
                     /gene="degP"
                     /locus_tag="OO326_000664"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5965169.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25206..26362
                     /gene="cdaR"
                     /locus_tag="OO326_000665"
                     /pseudo
     CDS             25206..26362
                     /gene="cdaR"
                     /locus_tag="OO326_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26451..26837)
                     /locus_tag="OO326_000666"
     CDS             complement(26451..26837)
                     /locus_tag="OO326_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5965170.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27151..27975)
                     /gene="dapD"
                     /locus_tag="OO326_000667"
     CDS             complement(27151..27975)
                     /gene="dapD"
                     /locus_tag="OO326_000667"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5965171.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28006..30678)
                     /gene="glnD"
                     /locus_tag="OO326_000668"
     CDS             complement(28006..30678)
                     /gene="glnD"
                     /locus_tag="OO326_000668"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5965172.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30740..31534)
                     /gene="map"
                     /locus_tag="OO326_000669"
     CDS             complement(30740..31534)
                     /gene="map"
                     /locus_tag="OO326_000669"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5965173.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31902..32627
                     /gene="rpsB"
                     /locus_tag="OO326_000670"
     CDS             31902..32627
                     /gene="rpsB"
                     /locus_tag="OO326_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5965174.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32885..33736
                     /gene="tsf"
                     /locus_tag="OO326_000671"
     CDS             32885..33736
                     /gene="tsf"
                     /locus_tag="OO326_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5965175.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33883..34608
                     /gene="pyrH"
                     /locus_tag="OO326_000672"
     CDS             33883..34608
                     /gene="pyrH"
                     /locus_tag="OO326_000672"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5965176.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34900..35457
                     /gene="frr"
                     /locus_tag="OO326_000673"
     CDS             34900..35457
                     /gene="frr"
                     /locus_tag="OO326_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5965177.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35549..36745
                     /gene="ispC"
                     /locus_tag="OO326_000674"
     CDS             35549..36745
                     /gene="ispC"
                     /locus_tag="OO326_000674"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5965178.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36934..37692
                     /gene="ispU"
                     /locus_tag="OO326_000675"
                     /gene_synonym="uppS"
     CDS             36934..37692
                     /gene="ispU"
                     /locus_tag="OO326_000675"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5965179.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37705..38562
                     /gene="cdsA"
                     /locus_tag="OO326_000676"
     CDS             37705..38562
                     /gene="cdsA"
                     /locus_tag="OO326_000676"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5965180.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38574..39926
                     /gene="rseP"
                     /locus_tag="OO326_000677"
     CDS             38574..39926
                     /gene="rseP"
                     /locus_tag="OO326_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5965181.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39956..42388
                     /gene="bamA"
                     /locus_tag="OO326_000678"
     CDS             39956..42388
                     /gene="bamA"
                     /locus_tag="OO326_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5965182.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42510..42995
                     /gene="skp"
                     /locus_tag="OO326_000679"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42510..42995
                     /gene="skp"
                     /locus_tag="OO326_000679"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5965183.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42999..44024
                     /gene="lpxD"
                     /locus_tag="OO326_000680"
     CDS             42999..44024
                     /gene="lpxD"
                     /locus_tag="OO326_000680"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5965184.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44129..44584
                     /gene="fabZ"
                     /locus_tag="OO326_000681"
     CDS             44129..44584
                     /gene="fabZ"
                     /locus_tag="OO326_000681"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5965185.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44588..45376
                     /gene="lpxA"
                     /locus_tag="OO326_000682"
     CDS             44588..45376
                     /gene="lpxA"
                     /locus_tag="OO326_000682"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5965186.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45376..46524
                     /gene="lpxB"
                     /locus_tag="OO326_000683"
     CDS             45376..46524
                     /gene="lpxB"
                     /locus_tag="OO326_000683"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5965187.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46521..47117
                     /gene="rnhB"
                     /locus_tag="OO326_000684"
     CDS             46521..47117
                     /gene="rnhB"
                     /locus_tag="OO326_000684"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5965188.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47154..50636
                     /gene="dnaE"
                     /locus_tag="OO326_000685"
     CDS             47154..50636
                     /gene="dnaE"
                     /locus_tag="OO326_000685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5965189.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEECLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50649..51608
                     /gene="accA"
                     /locus_tag="OO326_000686"
     CDS             50649..51608
                     /gene="accA"
                     /locus_tag="OO326_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5965190.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51707..53848
                     /gene="ldcC"
                     /locus_tag="OO326_000687"
     CDS             51707..53848
                     /gene="ldcC"
                     /locus_tag="OO326_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5965191.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53905..54294
                     /locus_tag="OO326_000688"
     CDS             53905..54294
                     /locus_tag="OO326_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5965192.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54359..55657
                     /gene="tilS"
                     /locus_tag="OO326_000689"
     CDS             54359..55657
                     /gene="tilS"
                     /locus_tag="OO326_000689"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5965193.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55706..55966)
                     /gene="rof"
                     /locus_tag="OO326_000690"
     CDS             complement(55706..55966)
                     /gene="rof"
                     /locus_tag="OO326_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5965194.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55953..56153)
                     /locus_tag="OO326_000691"
     CDS             complement(55953..56153)
                     /locus_tag="OO326_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5965195.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56319..56864
                     /locus_tag="OO326_000692"
     CDS             56319..56864
                     /locus_tag="OO326_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5965196.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56861..57283
                     /gene="arfB"
                     /locus_tag="OO326_000693"
     CDS             56861..57283
                     /gene="arfB"
                     /locus_tag="OO326_000693"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5965197.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57297..58007
                     /gene="nlpE"
                     /locus_tag="OO326_000694"
                     /gene_synonym="cutF"
     CDS             57297..58007
                     /gene="nlpE"
                     /locus_tag="OO326_000694"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5965198.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58162..58986)
                     /locus_tag="OO326_000695"
     CDS             complement(58162..58986)
                     /locus_tag="OO326_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5965199.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59039..60757)
                     /gene="proS"
                     /locus_tag="OO326_000696"
     CDS             complement(59039..60757)
                     /gene="proS"
                     /locus_tag="OO326_000696"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5965200.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60868..61575)
                     /gene="tsaA"
                     /locus_tag="OO326_000697"
     CDS             complement(60868..61575)
                     /gene="tsaA"
                     /locus_tag="OO326_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5965201.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61572..61976)
                     /gene="rcsF"
                     /locus_tag="OO326_000698"
     CDS             complement(61572..61976)
                     /gene="rcsF"
                     /locus_tag="OO326_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5965202.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62094..62909)
                     /gene="metQ"
                     /locus_tag="OO326_000699"
     CDS             complement(62094..62909)
                     /gene="metQ"
                     /locus_tag="OO326_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5965203.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62949..63602)
                     /gene="metI"
                     /locus_tag="OO326_000700"
     CDS             complement(62949..63602)
                     /gene="metI"
                     /locus_tag="OO326_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5965204.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63595..64626)
                     /gene="metN"
                     /locus_tag="OO326_000701"
     CDS             complement(63595..64626)
                     /gene="metN"
                     /locus_tag="OO326_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5965205.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64814..65389
                     /gene="gmhB"
                     /locus_tag="OO326_000702"
     CDS             64814..65389
                     /gene="gmhB"
                     /locus_tag="OO326_000702"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5965206.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65753..>66021
                     /locus_tag="OO326_000703"
     rRNA            65753..>66021
                     /locus_tag="OO326_000703"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15625 a        16345 c        18192 g        15859 t
ORIGIN      
        1 tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta aaatttttat
       61 taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata taaataaatt
      121 accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac gtctccccat
      181 caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat ccacgattct
      241 tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta ccaaacagca
      301 tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca cgaccctgtt
      361 gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa gtttcaagcg
      421 ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa
      481 acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc agggcagcat
      541 tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgt
      601 ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg
      661 ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa cctggaactc
      721 accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag ctcgcaaggc
      781 caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg cgcgtttacc
      841 ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg
      901 tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa
      961 agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg
     1021 gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt tatggatacg
     1081 cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc
     1141 cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt cacacaacag
     1201 cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt caaacaggcg
     1261 tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg cggtttccgg
     1321 gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac gtaccggatc
     1381 ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct tcagatcctt
     1441 cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca ggctgttgat
     1501 agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa
     1561 catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc
     1621 acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca gacggaaacg
     1681 gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg tggcgttagt
     1741 ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac
     1801 cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg cattttcact
     1861 gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc
     1921 gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac
     1981 tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg
     2041 ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcgggc
     2101 acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc
     2161 tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag taccgggcgt
     2221 ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgga
     2281 ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc
     2341 gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc tgtaacgccc
     2401 tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc acggcgatga
     2461 atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg cagcaggtca
     2521 gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc tggtccatga
     2581 tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgta
     2641 caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc acgataggcg
     2701 tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta atgttccagc
     2761 tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat atcttctatg
     2821 cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag cgcggcgatc
     2881 agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat atactgtgtg
     2941 tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacgtga
     3001 tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag cgttttgcag
     3061 tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg
     3121 caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt
     3181 ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg
     3241 gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg tgtaacggtg
     3301 cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc
     3361 agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg
     3421 atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc
     3481 cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag
     3541 atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg
     3601 tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta caatgtaacc
     3661 ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta agccagaatt
     3721 tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc
     3781 gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac cgcacgctgg
     3841 ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca
     3901 tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag
     3961 tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga gccgtatttt
     4021 acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt aaggatagcc
     4081 tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc gctctggctt
     4141 tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag
     4201 acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca gtgtagcgtt
     4261 aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc
     4321 gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat tgcgttagcg
     4381 cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg
     4441 tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc accgcctcgc
     4501 tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc
     4561 tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggaggctgac
     4621 gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg
     4681 tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct ttctctgcgc
     4741 tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc ggacgtcccg
     4801 cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt tctgcaggta
     4861 agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg ttatgaatta
     4921 ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt
     4981 tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg ccgcaggtat
     5041 tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc
     5101 atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg
     5161 tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga
     5221 tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc gaaggcgtgc
     5281 tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg
     5341 agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc gatgtgcaac
     5401 aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac aatgaccgct
     5461 tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg
     5521 aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt gcggtagcca
     5581 ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct ggcgtcggag
     5641 aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg gtgctctgcc
     5701 cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag
     5761 ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc atcgaaggta
     5821 ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg cgcacggggc
     5881 ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt gccgggcgcg
     5941 ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa gcagaacgcg
     6001 ccgcggcgca aagtgagccg aagatcttac aaagcgatct ttccggtttg ctgatggaat
     6061 tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa ccgccaacgg
     6121 tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag ggtgaacggc
     6181 ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta gcggcaatgc
     6241 tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc gccattctcg
     6301 aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag
     6361 cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag
     6421 acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc
     6481 gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc gatgccgacg
     6541 acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc agcgcctcgc
     6601 cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta caacgctgtc
     6661 cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag
     6721 cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt
     6781 cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt ttaagcgtgc
     6841 tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt
     6901 tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg ctggaaacgt
     6961 ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca ctcgacattt
     7021 atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc
     7081 ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataacc
     7141 cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat ccgacgatcg
     7201 cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg ccgctgcaaa
     7261 tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga
     7321 aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca ccgacgcgac
     7381 ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga gggccggggt
     7441 gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg gagaggggac
     7501 cgatcgagcg caaattttga gagatatctt cttctgtctt gtaacagaag aacagaaaat
     7561 cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac cgcgagccaa
     7621 ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt cgtcgttacg
     7681 aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg atgccgcgca
     7741 aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg ctatggctac
     7801 tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt tatctcgatc
     7861 aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca gtttatggcc
     7921 gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg aagctgctgg
     7981 aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt accgtgcagg
     8041 ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa gaagggcagg
     8101 tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat atggagaaca
     8161 accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc tcctcgccaa
     8221 acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg gtggatactt
     8281 tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc tactccattg
     8341 ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg agtacgctga
     8401 cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg cgtaaagcga
     8461 acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt attcttgagc
     8521 tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc
     8581 tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac cagcaggcgc
     8641 tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac ccgaaactgg
     8701 cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt attgatcaag
     8761 aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt ggggtgatct
     8821 ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa ctgggcgata
     8881 aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg gcccaggacg
     8941 cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt aagttgagcg
     9001 atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt gcgatggtcg
     9061 gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg
     9121 gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa atctatcgcc
     9181 tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc caggtctggt
     9241 caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg gtggatgcgt
     9301 tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg ctgcctgcgg
     9361 ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg gttccggcaa
     9421 tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc cagaccatcg
     9481 ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg gaagatggta
     9541 aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag gcggcgtatc
     9601 tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag cttggggcga
     9661 aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac gtagatacct
     9721 ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt gataacaacc
     9781 agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt tatctggcta
     9841 accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat atgggcgtgg
     9901 actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc tggaccagcg
     9961 atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc aatccgtttg
    10021 atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa gagcagaaag
    10081 acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa catctaagcg
    10141 tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa gatattaacc
    10201 ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc gtaccgcttg
    10261 cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct cgtttacgtt
    10321 attattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct cagccaaaac
    10381 actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg
    10441 cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc
    10501 aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct accggcacta
    10561 aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc gccgaagaga
    10621 tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg ggtgtctctg
    10681 ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt tttgcggcag
    10741 aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac ttctataacg
    10801 atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc ccggtttccg
    10861 tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag cgtccgacca
    10921 ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc cagactggtt
    10981 ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg accggtcttg
    11041 cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct attgcaccgg
    11101 cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac ttccagaacg
    11161 agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag ccgctgccga
    11221 acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc tattctcgta
    11281 atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt actgtgcgtc
    11341 agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt tacggcgtct
    11401 gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca gcggataaag
    11461 gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac ttctccgttg
    11521 atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg ctgaccggtg
    11581 tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac gactctgtgc
    11641 cactgttcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc aaagatccgg
    11701 caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt tatgttcagg
    11761 atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac tgggcagatc
    11821 aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa cagtttacct
    11881 ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc agctatagcg
    11941 aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca ccgtctaaag
    12001 gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt gtagttactg
    12061 gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag ggttccttct
    12121 tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg aaagcggcgc
    12181 tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa tacaccaccg
    12241 atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct tcgctgtggg
    12301 ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc ggtggtcgtt
    12361 atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt tatacggtcg
    12421 tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc aacgtggcgc
    12481 tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac acttatggct
    12541 gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc taatttctct
    12601 tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg aatacacgaa
    12661 tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct
    12721 tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga ttggtcacaa
    12781 cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc cgtcagaagg
    12841 ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt ttgcccgcaa
    12901 agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt
    12961 ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg cagatcgcga
    13021 aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc ggctggtcga
    13081 tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag
    13141 ccgttgtctg ttgctcgacg aaccgacttc agcgctggat atcgcccacc aggttgatgt
    13201 gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg ccgtgttgca
    13261 cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca gtggtgaaat
    13321 gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa tgatttatgg
    13381 catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt ttgtttattg
    13441 atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc gctttctccg
    13501 ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg tattgtggcg
    13561 ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta cggcgtggcg
    13621 gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc agtgattgac
    13681 gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc gtcgtttatg
    13741 gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc gccgggtcgc
    13801 ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc gctgacggaa
    13861 atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca atacgaagac
    13921 tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt actgctgacg
    13981 acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt ccaggaaatt
    14041 ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg gggcagtacc
    14101 gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg ttttgatcac
    14161 gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc catgccgttt
    14221 gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc aacgctttcg
    14281 gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg agtaaacgaa
    14341 ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg gcgctcacct
    14401 ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc tggtcgccgg
    14461 atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt ctggcgattt
    14521 cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa gtactgcgta
    14581 acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg gggattaccg
    14641 tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg ctggcagggg
    14701 cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg tcgccggtaa
    14761 cgctgatttt cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat cagttactgg
    14821 ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga acgctgacgc
    14881 aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt gtgatgctga
    14941 cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg gcgcgcaatc
    15001 tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt gtcatcagtg
    15061 cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg ccgctgctgg
    15121 caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg ttgattggtg
    15181 cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg tggatggaag
    15241 tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg ctgctgccgc
    15301 gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg actgaacgcc
    15361 aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct gtggtggtgg
    15421 cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg ttgctcgagg
    15481 atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc gtcatgctgg
    15541 cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc ccggaagtgc
    15601 tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg gtaccgggta
    15661 atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgacg ctgttgatca
    15721 ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg gcggggatgg
    15781 cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt gacccgcgaa
    15841 tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat gctcaggtct
    15901 ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc cgccgctggc
    15961 tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg ctgacgccga
    16021 cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg atgactattg
    16081 gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg ggctttcgac
    16141 ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg gtgttcgctg
    16201 actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg ctgtcgacct
    16261 ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa tagaatgcct
    16321 cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg cagcatcgat
    16381 ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcctgctt caaacgctga
    16441 cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa aacgctcgac
    16501 gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa agaagatacc
    16561 gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg ccgcttccag
    16621 cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc ccggctgcgc
    16681 gacttcattc agacaggcga aacccgctgc caccgcaatc gggttaccgg aaagcgtacc
    16741 cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac gaccaccgaa
    16801 tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat ctggcactac
    16861 ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca cttcatcgat
    16921 gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga actctggcag
    16981 cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg caatctcttg
    17041 cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac aggttaaggt
    17101 atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg tgagtgcgcc
    17161 agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt caaatttaat
    17221 gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg tcgcctcggt
    17281 gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca ccagttgcgc
    17341 cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg cggcttcaat
    17401 cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc aggaaccgac
    17461 ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg ctttttcgat
    17521 aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca caccgccagg
    17581 gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg gttcctgatt
    17641 cgtagaaaaa gtgaatggct gctattccat gttattcata acaagttaaa tactcgtcaa
    17701 acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag ttttgtgcat
    17761 ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat gccgtcatct
    17821 tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct ttagaaacac
    17881 cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag cgcgataaaa
    17941 ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg ctggctgcgg
    18001 ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg ctggtacata
    18061 ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga
    18121 tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg gggatcccgg
    18181 aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta ttgccggtga
    18241 aattctttgg cgggctgggg acactcggcg gaggcatggg gctagggcgc gaaggaccaa
    18301 ccgtgcagat cggcggcaat atcagtcgca tggtgcttga tattttccgc ctgaaaggtg
    18361 atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct gcggccttta
    18421 acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag tttcgctata
    18481 cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt atgtaccgga
    18541 tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg ccgcttaata
    18601 cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct atttttaata
    18661 aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat attaccaaat
    18721 gggtactgat ggggggggcg attggcggtc tgtgtggatt gctggggttt gtggcaccag
    18781 caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat ttcagcatgg
    18841 gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc ttctcttccg
    18901 gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg ggaaccgctt
    18961 tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg acgtttgcta
    19021 ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg gggatcattc
    19081 tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt accggtcttg
    19141 gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg attcttgcgc
    19201 gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca tcagccagcg
    19261 agaatacttg aacgaaatac cagggtatta gataatggcg attattattg ggttagaatt
    19321 tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca ctgccgctgg
    19381 agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa gataacccga
    19441 atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag tatggtttca
    19501 cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc gttggcctgg
    19561 tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac accgaaggac
    19621 tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt
    19681 cttcctttag catctaatct gttgttcatg tgccggataa actatccggc acatcttctc
    19741 tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc
    19801 cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt ccagtggtac
    19861 ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg cagttgcgta
    19921 gatttctgta cgtaaaatca tggggatttc gcggaccagt acatcacgaa tgatcccgcc
    19981 gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt ccgcattaaa
    20041 ggctttattc acgctaatgc cgacaaacac cgccagacca acggcgtcca gcaccggcaa
    20101 catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca tgctggtgac
    20161 catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt gatccagcgc
    20221 catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca gaacaccaaa
    20281 agggtccata cgcaattttc cggctaacaa aacgccgtag atggcaaata ccgctgtgcc
    20341 gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct gaaaaggtca
    20401 ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg ataatacgtg
    20461 ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg agctgttcac
    20521 cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca atagcctgtg
    20581 gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa
    20641 agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct tttccactgg
    20701 taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta tcagcatatt
    20761 gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg gccttgtctg
    20821 gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca atgcttgtcg
    20881 catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc cgctcggcat
    20941 tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt tccagattca
    21001 tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag tcggaatagc
    21061 tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa
    21121 gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac agggcgacca
    21181 gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc aaccatcagg
    21241 ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga ctgttgatcg
    21301 gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg gcagacatgg
    21361 gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg gcggattttc
    21421 gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg
    21481 ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc gtcagcttta
    21541 aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt gacatccgcg
    21601 tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt tggtgccagg
    21661 ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa cagcagagtg
    21721 gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac ctcggttcca
    21781 ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg gttttcgatt
    21841 ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat tttcatagat
    21901 ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat
    21961 ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa actggcatcg
    22021 tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga tcttcgagag
    22081 cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa agacccaggt
    22141 ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga tggaagtcca
    22201 gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc ttaaattgct
    22261 ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg agatgtcatg
    22321 cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag cgataaatga
    22381 ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga ctgacgatcg
    22441 ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg agctgcggcg
    22501 caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc gtctggtgca
    22561 tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa aatatacccg
    22621 tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga aaaagccggg
    22681 ttattatctt tctgaagaag cctatattgc ccgcttgcgt aaagaactta atttggcgct
    22741 ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct cctattgtgt
    22801 ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc tttatcatca
    22861 tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg tggttgaaaa
    22921 tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc agttttttat
    22981 gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac gatttattga
    23041 taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag atgccagcga
    23101 atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc
    23161 ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat tagagattta
    23221 tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa aagaacgggt
    23281 gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc atcggctggc
    23341 ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc tatgggaata
    23401 ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc tctatgcgtg
    23461 ggatgaatac cgacgtctga tggccgtaga acaataacca gtcttttgta aagacgaaca
    23521 ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa aacgaatctg
    23581 aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga aaaaaaacac
    23641 attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc tctctgcaac
    23701 ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg caccgatgct
    23761 cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa ccgttaatac
    23821 gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt tctgccagga
    23881 aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta atggcggcgg
    23941 ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg ataaaggcta
    24001 tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc agctgagcga
    24061 tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata tcgcgctgat
    24121 ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg atgcactgcg
    24181 cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga cggtaacttc
    24241 cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg aaaacttcat
    24301 ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta acctgaacgg
    24361 cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca tcggtatcgg
    24421 ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg aatacggcca
    24481 ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac tggcgaaagc
    24541 gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta attcctccgc
    24601 tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta agccgatcag
    24661 cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca aactgaccct
    24721 gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc agagcagcca
    24781 gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga tgagcaacaa
    24841 aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg ctgcgcagat
    24901 cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga aaaacatcgc
    24961 tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg
    25021 cgacagcacc atctacctgt taatgcagta atctccctca accccttcct gaatacggga
    25081 aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc atcatccttt
    25141 gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg cataacggag
    25201 gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg gcacgtacca
    25261 tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt atcggcagcg
    25321 gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt tcacagggac
    25381 gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg caggggatta
    25441 atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag
    25501 agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg ctggaacagt
    25561 cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg gtgatgaacc
    25621 tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc tggggatcga
    25681 tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc ttggcgtgga
    25741 cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg agcgtaataa
    25801 tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt tgaactcttt
    25861 tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta cccgcatgaa
    25921 agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg gtcctggcag
    25981 tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac agcggatgcc
    26041 agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg acagtttgcg
    26101 tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag cgatggacaa
    26161 taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc aaccgctggc
    26221 aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta atcgtatatc
    26281 ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt
    26341 acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg gctcattccc
    26401 ctcagcccga aggagagagg ggaatgcccg agttaaccgt caattcggga ttatttacgc
    26461 gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac tgactccagg
    26521 tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc acgctgacag
    26581 atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg gacttcttta
    26641 taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata tttaaactta
    26701 acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag aatatcgttg
    26761 ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat acccagactt
    26821 ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc ttattttagc
    26881 atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct tccagacttc
    26941 tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc catgggatgg
    27001 attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag atgaaaaagt
    27061 atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag aaagcatacg
    27121 ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca gttcgttaat
    27181 gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac agtagaggct
    27241 gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg ccggaacgcg
    27301 accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac caatgtatac
    27361 gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt cagagcgtgc
    27421 gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct ccagcacgcc
    27481 gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac aagaaccgac
    27541 ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt taacgtaaga
    27601 cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg ctggtggcac
    27661 aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac
    27721 tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa tacggaaaga
    27781 gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa ttttttccgc
    27841 tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt cgcgggtaac
    27901 ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt caataatgtt
    27961 ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc ctttatcgtt
    28021 tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt tattaagcgc
    28081 acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa tggttgtaat
    28141 tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc gcgccagcag
    28201 tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat gggtcggcaa
    28261 aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc gacggggctg
    28321 cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa taacctcatg
    28381 gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat ccatcgccat
    28441 accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc
    28501 ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa tctcggtgcc
    28561 tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat gctgtaataa
    28621 atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt tagcacgaca
    28681 gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag
    28741 tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg
    28801 tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct tccagctatt
    28861 ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca gatagcgcag
    28921 acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt cctgaatatc
    28981 gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg caaccagctg
    29041 tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct gagcaccgag
    29101 aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat
    29161 cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc gctggcgcgt
    29221 ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt catccaccgt
    29281 gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt gcggcatata
    29341 cgcgccgagc acgctatggc gatgcattgg caatagtccg cgccgcaccg ctccggggtg
    29401 acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg gttgttgcag
    29461 atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg tgatcgcact
    29521 gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca taaatagtgt
    29581 ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga gtggacgtgg
    29641 tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca gcatctggtt
    29701 gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct cgaccggttc
    29761 gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat cgaataacag
    29821 acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc acagtatatg
    29881 cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc cgaccatttc
    29941 atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt gaatatcgcg
    30001 caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc catggtaacg
    30061 ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg aaggccagaa
    30121 tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc caattaataa
    30181 gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca acatgcactc
    30241 ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca gcgttaacag
    30301 ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc
    30361 gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa ccaatgccag
    30421 gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct ggtctatgaa
    30481 ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg catcacccag
    30541 ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt catcacgggg
    30601 ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt ttgcgtactg
    30661 ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat taaaaaagcc
    30721 ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg gtgtcatcct
    30781 tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc tcatattgtg
    30841 cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg cggatctctt
    30901 ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc agcacgacgt
    30961 tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg cgaccaatac
    31021 cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat ttctgaatcg
    31081 ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc aggtacaggc
    31141 tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg acgataaaca
    31201 ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg ttaacgatat
    31261 cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact tcattaatag
    31321 agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt
    31381 cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa
    31441 catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg cgcatttttt
    31501 cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag cgtcggtgat
    31561 accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat
    31621 tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc gacgtctgat
    31681 gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat aaagcgcgcc
    31741 ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc actttgtgta
    31801 acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta atatgggata
    31861 cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact gtttccatgc
    31921 gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg aacccgaaaa
    31981 tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt gagaaaactg
    32041 taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc aaaggtaaaa
    32101 tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct
    32161 gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac tggaaaaccg
    32221 ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac ggtactttcg
    32281 acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca
    32341 gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta atcgatgctg
    32401 accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta tttgctatcg
    32461 ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac gacgacgcaa
    32521 tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc
    32581 aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa ggcttgataa
    32641 ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc cgggagctta
    32701 catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc gaaatatgaa
    32761 gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg catatggccc
    32821 cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct ccgaggattt
    32881 tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta ctggcgcagg
    32941 catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc tggcaatcga
    33001 aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga
    33061 cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag ttaactgcca
    33121 gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag ttctggacgc
    33181 agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg aagaagaacg
    33241 tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga
    33301 aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa
    33361 aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa gcaagccaga
    33421 attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc aggtacagct
    33481 ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg
    33541 catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta tggaaccaag
    33601 caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct tcatccgttt
    33661 cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag ttgctgcgat
    33721 gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt tttgtgccca
    33781 tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta
    33841 gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac caatgcaaaa
    33901 cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg cactgaaggc
    33961 ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga actggttgaa
    34021 ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg cgctggtctg
    34081 gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg
    34141 aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc
    34201 gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat cagcctgttg
    34261 cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt taccaccgac
    34321 tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa
    34381 gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa
    34441 ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc ggccttcacg
    34501 ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc gggtgcgctg
    34561 cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt cccgtgatgg
    34621 ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt tataaggcgt
    34681 tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta caaaagtacg
    34741 tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta ataatccaga
    34801 cgattacccg taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact
    34861 gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa
    34921 gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat cagcaaaata
    34981 cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg
    35041 ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac tctgaaaatc
    35101 aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc ctccgatctc
    35161 ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa
    35221 gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca
    35281 gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag
    35341 atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca
    35401 atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt
    35461 cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt
    35521 tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg
    35581 ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt
    35641 tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc
    35701 ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga tgctacagca
    35761 acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct
    35821 tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt tacctacgct
    35881 tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg
    35941 cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag
    36001 cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc
    36061 tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg
    36121 tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc gtcatccgaa
    36181 ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca aaggtctgga
    36241 atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca
    36301 cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc tggcgcagct
    36361 gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga atcgcgtgaa
    36421 ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga
    36481 ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag gccaggcagc
    36541 gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg cgcaacaaat
    36601 ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga
    36661 accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag tcgccagaaa
    36721 agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt
    36781 agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag
    36841 gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta
    36901 ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca accacttagc
    36961 gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg caatggtcgc
    37021 tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc
    37081 cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct gtatgccttt
    37141 agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg
    37201 tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt
    37261 ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta
    37321 acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg acgttgggat
    37381 atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat
    37441 cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc ccctgtagat
    37501 ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg gcaaattgcc
    37561 tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa
    37621 ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat
    37681 gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta
    37741 atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc cattgtaacg
    37801 ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt taccactcgt
    37861 tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat gctttttctg
    37921 ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact ttgggcttcg
    37981 ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc cgcagcaatc
    38041 tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt tcccttcttc
    38101 tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag tggcgcaata
    38161 tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata tatgtttggc
    38221 aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc
    38281 tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat gtgggcgaat
    38341 ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt agcctcagtg
    38401 ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga cagcggtcat
    38461 ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc tgcggtaccg
    38521 gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt aatatgctga
    38581 gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc accgtgcatg
    38641 aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag
    38701 ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat gttatcgccc
    38761 tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg
    38821 aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg attattgccg
    38881 caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt tttattattg
    38941 gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata gctgcggaag
    39001 cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg cctgattggg
    39061 atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc attacagtag
    39121 cgccatttga cagcgaccaa cggcgggatg taaagctcga tttacgtcac tgggcgtttg
    39181 agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg
    39241 aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt ttgcaagcag
    39301 gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc
    39361 ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag gggagtccct
    39421 tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg
    39481 gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc cagtatgggc
    39541 cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag ctgacggtca
    39601 gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt gggccgatct
    39661 ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac ctgccgtttc
    39721 ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc gtacttgacg
    39781 gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta tccgagcggg
    39841 ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg gggcttgcac
    39901 ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa taacgatggc
    39961 gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat acggtgctga
    40021 agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc
    40081 cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata tcagtaatac
    40141 cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga
    40201 tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt tctccggtaa
    40261 caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg
    40321 cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag acttctacta
    40381 cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg
    40441 tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc aaattaacat
    40501 tgttggtaac catgctttca ccaccgacga actgatctct catttccaac tgcgtgacga
    40561 agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac tggcgggcga
    40621 tcttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca acatcgactc
    40681 tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga acatcaccga
    40741 aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg ggcactccgc
    40801 tgaaattgag caactgacta agatcgagcc gggcgagctg tataacggca ctaaagtgac
    40861 caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct atccgcgcgt
    40921 acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg tgaacgttga
    40981 tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga
    41041 tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga gcgatctggt
    41101 cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg ataccgatac
    41161 ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga aagaacgtaa
    41221 cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga gcttccaggc
    41281 tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca acgggaccaa
    41341 aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg
    41401 cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg ccgacctgtc
    41461 cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga ttaacgaata
    41521 taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca tgcagcctca
    41581 ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct ctgatcagga
    41641 taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata acaagcttga
    41701 ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag tgaccattcc
    41761 tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg tgccgatcga
    41821 tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg atggtttagg
    41881 cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca ccgtgcgtgg
    41941 cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg ccagtaatta
    42001 tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc
    42061 ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca tcaccccgac
    42121 gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct gggatatggg
    42181 taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg actatagtga
    42241 tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc cattggggcc
    42301 gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg cagaacagtt
    42361 ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta gtggtagtgt
    42421 agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac tgcaccctcc
    42481 ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct gcaggtcttg
    42541 gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca
    42601 gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa aatgagttca
    42661 aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa atgaaaaaac
    42721 tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg atggctcagc
    42781 gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg
    42841 aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt gccaacagcc
    42901 aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc gatgtaaaag
    42961 acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt cgactggctg
    43021 atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc atcaccggcg
    43081 ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac ccaaaatacc
    43141 gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac gatcttcctt
    43201 tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg cgcatggcgc
    43261 aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg gtgatcgacg
    43321 cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg
    43381 ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa
    43441 tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc cagatcggtc
    43501 agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt tatgccaacg
    43561 atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc gatcgcgtgg
    43621 agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt attggcaatg
    43681 gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc gacaatacgg
    43741 cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac tgcatgatcg
    43801 gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg gttacgggca
    43861 tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc attccgctgc
    43921 aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat gacatgagca
    43981 agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt
    44041 cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt attattgtcg
    44101 tttcttatat tttgacagga agagtatctt gactactaac actcatactc tgcagattga
    44161 agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc gcgtgctgga
    44221 ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt
    44281 ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg aagcaatggc
    44341 acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta
    44401 ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat
    44461 gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc
    44521 tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc
    44581 ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg aagagggcgc
    44641 gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc atgtcgaaat
    44701 tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta aaattggccg
    44761 cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt tgaaatatgc
    44821 tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat
    44881 tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca acttactgat
    44941 gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc tcgccaacaa
    45001 cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg gcatgaccgc
    45061 agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct ccggtgtggc
    45121 gcaggacgtc cctccttatg ttattgcgca gggtaaccac gcaacgccgt tcggtgtcaa
    45181 tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta tccgcaatgc
    45241 gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga
    45301 actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg
    45361 cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt cgccggagaa
    45421 acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg tgtgcccaac
    45481 gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga agcctggtac
    45541 gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta
    45601 ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga tgtttttgtt
    45661 ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa gcagggtatc
    45721 aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg cgttttcaaa
    45781 ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac
    45841 aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat gccgttagat
    45901 ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca ctgtctggca
    45961 ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt cttgaaaact
    46021 gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct ggtgaatgcc
    46081 aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct gtcggttcat
    46141 ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact actggcgtcg
    46201 gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg atatcgcatg
    46261 aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt ctcgctgcca
    46321 aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg tgagccgcaa
    46381 aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca cgcgatgcac
    46441 gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa
    46501 gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag ctggttgcgg
    46561 gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc
    46621 ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc gaaaaacgcc
    46681 gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg
    46741 aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg atgcagcgtg
    46801 ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac cgctgcccga
    46861 aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca gaaatcagtg
    46921 ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt
    46981 tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat ctggaaaaac
    47041 tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc aaacgcgcac
    47101 tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg
    47161 aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat ggcttggcca
    47221 aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg gcgatcaccg
    47281 atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc gcagggatta
    47341 agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat gagttaaccc
    47401 acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg ctgatctcaa
    47461 aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg
    47521 aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc
    47581 ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc
    47641 cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa agctatctgc
    47701 acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact aacgatgtgc
    47761 gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc cacgacggct
    47821 ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat atgcgtagcg
    47881 aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac accgttgaga
    47941 tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg cagttcccga
    48001 ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc ctggaagagc
    48061 gtctggcatt tttattccct gacgaggaag aatgtcttaa acgccgcccg gaatatgacg
    48121 aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc tacttcctca
    48181 tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg ccaggccgtg
    48241 gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc gatccgctgg
    48301 aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg cctgacttcg
    48361 acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg gacatgtacg
    48421 gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa gcggtgatcc
    48481 gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc tcgaaactga
    48541 tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg cagctgccgg
    48601 aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc aaactggaag
    48661 gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg accaaaatta
    48721 ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc cagtttgata
    48781 aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg cgtacgctca
    48841 ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat ggcgagccac
    48901 cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg ctgcaacgct
    48961 cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc
    49021 tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc cctggtccgt
    49081 tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa gagatctcct
    49141 atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca acctacggca
    49201 ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt tataccctcg
    49261 gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc
    49321 agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa ctggcgatga
    49381 aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg cactctgcgg
    49441 cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg gcggagttta
    49501 tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc ctggtggatg
    49561 agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt ctttaccatt
    49621 tccacgtcaa cgacgatggc gaaatcgtgt atggtattgg cgccatcaaa ggggtaggtg
    49681 aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc cgcgaactgt
    49741 ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga
    49801 tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg
    49861 gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt caggccgata
    49921 tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc agctgccaac
    49981 cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg tacctgaccg
    50041 gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga
    50101 aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc gttgttgccg
    50161 cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt
    50221 ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag caattgctgg
    50281 aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc agcggtgggc
    50341 ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg
    50401 ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac cgactccgtc
    50461 agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat cagagggcgg
    50521 atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat
    50581 taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt gactaataca
    50641 ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag ctggaagcga
    50701 aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt aacatcgatg
    50761 aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc
    50821 tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat accctggatt
    50881 acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg tatgcagacg
    50941 ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg atcattggtc
    51001 atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag
    51061 aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag atgcctatca
    51121 tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt
    51181 ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg gtagtttgta
    51241 cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc gataaagtga
    51301 atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg tccattctgt
    51361 ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc
    51421 tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc gctcaccgta
    51481 acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg gccgatctcg
    51541 atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt
    51601 acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac
    51661 ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga acatcattgc
    51721 cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac tggagtcggc
    51781 gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt tgctgaaatt
    51841 tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt acagtctcga
    51901 tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct tcatcaacac
    51961 ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt tttttgaata
    52021 tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca acgaatatct
    52081 tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag agcggaagta
    52141 caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc cggttggctg
    52201 tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta tttcggtcac
    52261 cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag agtacatcgc
    52321 gcggactttt ggcgcggaac agagttatat cgttaccaac ggaatatcga cgtcgaacaa
    52381 aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc gcaactgcca
    52441 taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac
    52501 gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc gcgacagcat
    52561 cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg tgatcaccaa
    52621 ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc tggatgtccc
    52681 gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa tctaccaggg
    52741 taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa cgcaatcgac
    52801 ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag gcgagtatga
    52861 cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca gttatcccat
    52921 tgttgcttcg gttaagacgg cggcggcgat gctgcgtggt aatccgggca aacggctgat
    52981 taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga
    53041 gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag ccgaatgcag
    53101 gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg atcatatgtt
    53161 tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg gcaatatgag
    53221 tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg ggatcgtagt
    53281 agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg ataaaaccaa
    53341 agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc tcaacctgcg
    53401 gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc gcaatatgcg
    53461 tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc tttccggttt
    53521 gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc aggcatggca
    53581 gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg gtagagtatc
    53641 ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag gagaaatgct
    53701 gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg tagggcaaca
    53761 ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg gcgtttaccg
    53821 cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc gagtaacgtg
    53881 ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca tattgcgatt
    53941 attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg tttcacgctg
    54001 caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc gcttaatggg
    54061 caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag ccgaccggaa
    54121 gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc agtggcgcac
    54181 cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc
    54241 ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca gtaaggcttg
    54301 tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat
    54361 gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg cctttagcgg
    54421 cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg aaaatccggg
    54481 cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg atgcctgggt
    54541 tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg aacgcgtaca
    54601 acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcacgtt atcaggcatt
    54661 tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg acgatcaatg
    54721 tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt cggctatggc
    54781 ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc gcacgcgggg
    54841 ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg aaagtaatca
    54901 ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat tgcagcagcg
    54961 ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg aacaagagag
    55021 tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc aggggacgct
    55081 gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta ttcgccgctg
    55141 gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca tctggcagga
    55201 agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct ttgaaatccg
    55261 ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg aaaccattgt
    55321 gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa ccgtacagct
    55381 tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa
    55441 agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga agaaaatctg
    55501 gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt tttatggaga
    55561 aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa
    55621 tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat aagacgcatc
    55681 aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta ccgatttcag
    55741 ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca gcggcctcga
    55801 cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc tgcaattttt
    55861 cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc tcaagattat
    55921 cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc gctcaccagt
    55981 aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc gtcgtcggca
    56041 gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta tcctaagtct
    56101 ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc cacatgccct
    56161 cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga taaactctgt
    56221 ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac tgcacgtatt
    56281 ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt
    56341 ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat gcctctctga
    56401 cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg ctggcgtggc
    56461 tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat gatgagccgg
    56521 aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg gggctgccgg
    56581 atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg tttacctata
    56641 atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg cagtttgcca
    56701 atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc tttgccgatc
    56761 gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg gatgataaga
    56821 ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga tttcccgaca
    56881 tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg gcgctggcgg
    56941 gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc gggcgtccag
    57001 cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga tcagcagtga
    57061 tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc gcgaagccgc
    57121 tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag cccgacgacc
    57181 cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac aaaaatcaag
    57241 cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag gaatggatgg
    57301 tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg atgggatgta
    57361 ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg aaaccgatgc
    57421 cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa atctctctgt
    57481 tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct cgtgaagaac
    57541 cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg
    57601 acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag atgctcgatc
    57661 gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca caatccagtt
    57721 tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat gcggcgacct
    57781 tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag ctggagcgta
    57841 gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta gaaggtcact
    57901 ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg cccgatacgg
    57961 cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc tgcttaaggc
    58021 agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc atataatgca
    58081 acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc atccggcatt
    58141 ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg ccttgatata
    58201 aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa acgcagagac
    58261 atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt gcgccagtgt
    58321 aagtggatgc ccggatttgg caaatgcatc agtgacaaat tccgaacaaa accacgactt
    58381 tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga cgcactgttg
    58441 gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca taaagggaat
    58501 aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg caaacgcggt
    58561 gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa gcttatcact
    58621 atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac
    58681 gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga agacgcggat
    58741 tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg
    58801 tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga atttgacagc
    58861 ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc tgatatcaac
    58921 ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg cctttggttt
    58981 atccatttta tacaatccat gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc
    59041 agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt aactgtttct
    59101 cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg tcgcccagca
    59161 caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc ggacgctctt
    59221 tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac agtttctcag
    59281 caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc acctggaacg
    59341 gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca atcgccgcag
    59401 ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg ttacggccat
    59461 cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc tggaagatgt
    59521 gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg cttggatcgc
    59581 cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga tcccagttga
    59641 taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc atcgccgcaa
    59701 cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa cccggaccgg
    59761 ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg
    59821 gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca
    59881 gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt ttctcaatcg
    59941 gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta tcaaccagcg
    60001 tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa
    60061 tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca
    60121 gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc
    60181 ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg
    60241 tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc aggaattcgc
    60301 gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc tggatctgat
    60361 agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg tcagtgataa
    60421 cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca aaacgcagca
    60481 gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct ggctgaacca
    60541 ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg atgttttcga
    60601 cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag gccagcttgc
    60661 ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct
    60721 ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc cagttggaag
    60781 gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa
    60841 gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc
    60901 tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat aggttttgcc
    60961 cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt cgcggataaa
    61021 cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga cctctgcggt
    61081 aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac tggcatcggg
    61141 aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac catcgaccag
    61201 atccagactg ccgagcttca gaatcacgct gcctttatgg caaacaacct ctttcagctc
    61261 taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga aaacccccat
    61321 tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc cttccatcgt
    61381 ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc gaacggcgtc
    61441 ggcctggttg taggaagcta tgagatgcag ttcgccgttg gcgcttttta ccagacctgg
    61501 ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta tttgctcgaa
    61561 ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac agcctgacga
    61621 tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc attggctttc
    61681 attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg cggagagtcc
    61741 tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa cggtttgccg
    61801 actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt
    61861 gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt
    61921 aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc acgcatagta
    61981 tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga
    62041 cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa aaattaccag
    62101 cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc atcagactga
    62161 taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg cgttacgatc
    62221 aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc cggagtcagg
    62281 ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc gtcgtccaga
    62341 gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg gttctcaaca
    62401 acatcaagaa cggtcggcag caggccaacg ccatctttca gtttgatcaa gcccactttt
    62461 tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc aacctgcgaa
    62521 ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat cggataaaca
    62581 aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg atcaaggtac
    62641 ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga
    62701 acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt cgcaactttc
    62761 tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt aatgtggttt
    62821 ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag ggctcccact
    62881 gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta tttatgttgt
    62941 gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcaaa ctgaattaaa
    63001 taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt gtagccgatg
    63061 tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc acccatcgcg
    63121 gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc cggcaacgct
    63181 tccggtaaca gcactttaca gacgatctgc attggcgtgg cacccattgc gcgggaagct
    63241 tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc aataaacggt
    63301 gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt accgacaata
    63361 acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga acggaaaatg
    63421 ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg ccccggacgc
    63481 gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa tccggatacg
    63541 aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat cggctcagac
    63601 ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt
    63661 atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc caccggcgta
    63721 gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag
    63781 cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca cgcagtcagt
    63841 aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat gcagggtcga
    63901 ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc
    63961 ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac gcttcacaac
    64021 gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa tgtctttcag
    64081 cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt catcacacag
    64141 caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc
    64201 ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg acagcaattc
    64261 cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg gcagagccac
    64321 gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca taccaatctg
    64381 gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc catcgaccag
    64441 cacgctaccc tcggttgggc gctccagcaa gtttacacaa cgtataagcg tactcttacc
    64501 cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca ggctgacgtt
    64561 gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat tcgaaagttt
    64621 tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat gcctgaaacg
    64681 ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca atcaggttta
    64741 tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat gccttattaa
    64801 ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg atggcaccat
    64861 taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg acggtgttat
    64921 tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc
    64981 tggcattgct cgcggtaaat ttaccgaagc acagtttgaa acgctgaccg agtggatgga
    65041 ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc cgcatcatcc
    65101 gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac atccggggat
    65161 gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata tggtgggcga
    65221 taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg
    65281 tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt taaatagcct
    65341 ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat taaaagatga
    65401 acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc ctataatgcg
    65461 cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga
    65521 aaaaatcctg aaattcaggg ttgactctga aataggaaag tgtgatatac gccacctcgc
    65581 gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    65641 gggcactcga agatacggat tcttaacgtc gcaagacgat aaatgaatac caagtctcaa
    65701 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    65761 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    65821 gtaacaggaa gcgcttgctg cttcgctgac gagtggcgga cgggtgagta atgtctggga
    65881 agctgcctga tggaggggga taactactgg aaacggtagc taataccgca taacgtcgca
    65941 agaccaaaga gggggacctt cgggcctctt gccatcggat gtgcccagat gggattagct
    66001 tgttggtggg gtaacggctc a
//
LOCUS       DAJTRW010000010        64117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRW010000010 DAJTRW010000000
VERSION     DAJTRW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            123..566
                     /locus_tag="OO326_000704"
     CDS             123..566
                     /locus_tag="OO326_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5965207.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            802..1620
                     /gene="ybhA"
                     /locus_tag="OO326_000705"
     CDS             802..1620
                     /gene="ybhA"
                     /locus_tag="OO326_000705"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5965208.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1621..2679)
                     /gene="modC"
                     /locus_tag="OO326_000706"
     CDS             complement(1621..2679)
                     /gene="modC"
                     /locus_tag="OO326_000706"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5965209.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2682..3371)
                     /gene="modB"
                     /locus_tag="OO326_000707"
     CDS             complement(2682..3371)
                     /gene="modB"
                     /locus_tag="OO326_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5965210.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3371..4144)
                     /gene="modA"
                     /locus_tag="OO326_000708"
     CDS             complement(3371..4144)
                     /gene="modA"
                     /locus_tag="OO326_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5965211.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4311..4460)
                     /gene="acrZ"
                     /locus_tag="OO326_000709"
     CDS             complement(4311..4460)
                     /gene="acrZ"
                     /locus_tag="OO326_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5965212.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4589..5377
                     /gene="modE"
                     /locus_tag="OO326_000710"
     CDS             4589..5377
                     /gene="modE"
                     /locus_tag="OO326_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5965213.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5445..6917
                     /gene="modF"
                     /locus_tag="OO326_000711"
     CDS             5445..6917
                     /gene="modF"
                     /locus_tag="OO326_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5965214.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7178..8194
                     /gene="galE"
                     /locus_tag="OO326_000712"
     CDS             7178..8194
                     /gene="galE"
                     /locus_tag="OO326_000712"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5965215.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8204..9250
                     /gene="galT"
                     /locus_tag="OO326_000713"
     CDS             8204..9250
                     /gene="galT"
                     /locus_tag="OO326_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5965216.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9254..10402
                     /gene="galK"
                     /locus_tag="OO326_000714"
     CDS             9254..10402
                     /gene="galK"
                     /locus_tag="OO326_000714"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5965217.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10396..11436
                     /gene="galM"
                     /locus_tag="OO326_000715"
     CDS             10396..11436
                     /gene="galM"
                     /locus_tag="OO326_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5965218.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11639..12391
                     /gene="gpmA"
                     /locus_tag="OO326_000716"
     CDS             11639..12391
                     /gene="gpmA"
                     /locus_tag="OO326_000716"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5965219.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12558..13610)
                     /gene="aroG"
                     /locus_tag="OO326_000717"
     CDS             complement(12558..13610)
                     /gene="aroG"
                     /locus_tag="OO326_000717"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5965220.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13926..14306
                     /locus_tag="OO326_000718"
     CDS             13926..14306
                     /locus_tag="OO326_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5965221.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14420..15361
                     /gene="zitB"
                     /locus_tag="OO326_000719"
     CDS             14420..15361
                     /gene="zitB"
                     /locus_tag="OO326_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5965222.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15358..16077)
                     /gene="pnuC"
                     /locus_tag="OO326_000720"
     CDS             complement(15358..16077)
                     /gene="pnuC"
                     /locus_tag="OO326_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5965223.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16115..17158)
                     /gene="nadA"
                     /locus_tag="OO326_000721"
     CDS             complement(16115..17158)
                     /gene="nadA"
                     /locus_tag="OO326_000721"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5965224.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17407..17482)
                     /locus_tag="OO326_000722"
     tRNA            complement(17407..17482)
                     /locus_tag="OO326_000722"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17447..17449),aa:Lys,seq:ttt)
     gene            complement(17486..17561)
                     /locus_tag="OO326_000723"
     tRNA            complement(17486..17561)
                     /locus_tag="OO326_000723"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17526..17528),aa:Val,seq:tac)
     gene            complement(17613..17688)
                     /locus_tag="OO326_000724"
     tRNA            complement(17613..17688)
                     /locus_tag="OO326_000724"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17653..17655),aa:Lys,seq:ttt)
     gene            complement(17691..17766)
                     /locus_tag="OO326_000725"
     tRNA            complement(17691..17766)
                     /locus_tag="OO326_000725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17731..17733),aa:Val,seq:tac)
     gene            complement(17902..17977)
                     /locus_tag="OO326_000726"
     tRNA            complement(17902..17977)
                     /locus_tag="OO326_000726"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17942..17944),aa:Lys,seq:ttt)
     gene            complement(18142..18933)
                     /gene="cpoB"
                     /locus_tag="OO326_000727"
     CDS             complement(18142..18933)
                     /gene="cpoB"
                     /locus_tag="OO326_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5965225.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18943..19464)
                     /gene="pal"
                     /locus_tag="OO326_000728"
     CDS             complement(18943..19464)
                     /gene="pal"
                     /locus_tag="OO326_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5965226.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19499..20791)
                     /gene="tolB"
                     /locus_tag="OO326_000729"
     CDS             complement(19499..20791)
                     /gene="tolB"
                     /locus_tag="OO326_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5965227.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20924..22165)
                     /gene="tolA"
                     /locus_tag="OO326_000730"
     CDS             complement(20924..22165)
                     /gene="tolA"
                     /locus_tag="OO326_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5965228.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22230..22658)
                     /gene="tolR"
                     /locus_tag="OO326_000731"
     CDS             complement(22230..22658)
                     /gene="tolR"
                     /locus_tag="OO326_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5965229.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22662..23354)
                     /gene="tolQ"
                     /locus_tag="OO326_000732"
     CDS             complement(22662..23354)
                     /gene="tolQ"
                     /locus_tag="OO326_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5965230.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23351..23755)
                     /gene="ybgC"
                     /locus_tag="OO326_000733"
     CDS             complement(23351..23755)
                     /gene="ybgC"
                     /locus_tag="OO326_000733"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5965231.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23905..24198)
                     /gene="ybgE"
                     /locus_tag="OO326_000734"
     CDS             complement(23905..24198)
                     /gene="ybgE"
                     /locus_tag="OO326_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5965232.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24198..24311)
                     /gene="cydX"
                     /locus_tag="OO326_000735"
     CDS             complement(24198..24311)
                     /gene="cydX"
                     /locus_tag="OO326_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5965233.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24326..25465)
                     /gene="cydB"
                     /locus_tag="OO326_000736"
     CDS             complement(24326..25465)
                     /gene="cydB"
                     /locus_tag="OO326_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5965234.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25481..27049)
                     /gene="cydA"
                     /locus_tag="OO326_000737"
     CDS             complement(25481..27049)
                     /gene="cydA"
                     /locus_tag="OO326_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5965235.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27898..30531)
                     /gene="mngB"
                     /locus_tag="OO326_000738"
     CDS             complement(27898..30531)
                     /gene="mngB"
                     /locus_tag="OO326_000738"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5965236.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30549..32465)
                     /gene="mngA"
                     /locus_tag="OO326_000739"
     CDS             complement(30549..32465)
                     /gene="mngA"
                     /locus_tag="OO326_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5965237.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32635..33357
                     /gene="mngR"
                     /locus_tag="OO326_000740"
     CDS             32635..33357
                     /gene="mngR"
                     /locus_tag="OO326_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5965238.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33460..34329)
                     /gene="sucD"
                     /locus_tag="OO326_000741"
     CDS             complement(33460..34329)
                     /gene="sucD"
                     /locus_tag="OO326_000741"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5965239.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34329..35495)
                     /gene="sucC"
                     /locus_tag="OO326_000742"
     CDS             complement(34329..35495)
                     /gene="sucC"
                     /locus_tag="OO326_000742"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5965240.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35589..36806)
                     /gene="odhB"
                     /locus_tag="OO326_000743"
     CDS             complement(35589..36806)
                     /gene="odhB"
                     /locus_tag="OO326_000743"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5965241.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36821..39622)
                     /gene="sucA"
                     /locus_tag="OO326_000744"
     CDS             complement(36821..39622)
                     /gene="sucA"
                     /locus_tag="OO326_000744"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5965242.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39923..40639)
                     /gene="sdhB"
                     /locus_tag="OO326_000745"
     CDS             complement(39923..40639)
                     /gene="sdhB"
                     /locus_tag="OO326_000745"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5965243.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40655..42421)
                     /gene="sdhA"
                     /locus_tag="OO326_000746"
     CDS             complement(40655..42421)
                     /gene="sdhA"
                     /locus_tag="OO326_000746"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5965244.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42421..42768)
                     /gene="sdhD"
                     /locus_tag="OO326_000747"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42421..42768)
                     /gene="sdhD"
                     /locus_tag="OO326_000747"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5965245.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42762..43166)
                     /gene="sdhC"
                     /locus_tag="OO326_000748"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42762..43166)
                     /gene="sdhC"
                     /locus_tag="OO326_000748"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5965246.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43547..43654
                     /locus_tag="OO326_000749"
     CDS             43547..43654
                     /locus_tag="OO326_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965247.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43860..45143
                     /gene="gltA"
                     /locus_tag="OO326_000750"
     CDS             43860..45143
                     /gene="gltA"
                     /locus_tag="OO326_000750"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5965248.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45534..46100
                     /locus_tag="OO326_000751"
     CDS             45534..46100
                     /locus_tag="OO326_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5965249.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46151..48610
                     /locus_tag="OO326_000752"
     CDS             46151..48610
                     /locus_tag="OO326_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5965250.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48625..49356
                     /locus_tag="OO326_000753"
     CDS             48625..49356
                     /locus_tag="OO326_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5965251.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49353..50429
                     /locus_tag="OO326_000754"
                     /pseudo
     CDS             49353..50429
                     /locus_tag="OO326_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50581..51627
                     /locus_tag="OO326_000755"
     CDS             50581..51627
                     /locus_tag="OO326_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5965252.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51624..52415)
                     /gene="nei"
                     /locus_tag="OO326_000756"
     CDS             complement(51624..52415)
                     /gene="nei"
                     /locus_tag="OO326_000756"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5965253.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52451..53185)
                     /gene="pxpA"
                     /locus_tag="OO326_000757"
     CDS             complement(52451..53185)
                     /gene="pxpA"
                     /locus_tag="OO326_000757"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5965254.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53175..54107)
                     /gene="pxpC"
                     /locus_tag="OO326_000758"
                     /pseudo
     CDS             complement(53175..54107)
                     /gene="pxpC"
                     /locus_tag="OO326_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54101..54757)
                     /gene="pxpB"
                     /locus_tag="OO326_000759"
     CDS             complement(54101..54757)
                     /gene="pxpB"
                     /locus_tag="OO326_000759"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5965255.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54780..55523)
                     /gene="ybgI"
                     /locus_tag="OO326_000760"
     CDS             complement(54780..55523)
                     /gene="ybgI"
                     /locus_tag="OO326_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5965256.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55794..57275
                     /gene="dtpD"
                     /locus_tag="OO326_000761"
     CDS             55794..57275
                     /gene="dtpD"
                     /locus_tag="OO326_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5965257.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57425..58843)
                     /gene="phrB"
                     /locus_tag="OO326_000762"
     CDS             complement(57425..58843)
                     /gene="phrB"
                     /locus_tag="OO326_000762"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5965258.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58840..59349)
                     /locus_tag="OO326_000763"
     CDS             complement(58840..59349)
                     /locus_tag="OO326_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5965259.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59478..59765
                     /locus_tag="OO326_000764"
     CDS             59478..59765
                     /locus_tag="OO326_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5965260.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59820..60200)
                     /locus_tag="OO326_000765"
     CDS             complement(59820..60200)
                     /locus_tag="OO326_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965261.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60203..63589)
                     /locus_tag="OO326_000766"
     CDS             complement(60203..63589)
                     /locus_tag="OO326_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5965262.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63608..63811)
                     /locus_tag="OO326_000767"
                     /pseudo
     CDS             complement(63608..63811)
                     /locus_tag="OO326_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15662 a        17267 c        15610 g        15578 t
ORIGIN      
        1 aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt cgactgttaa
       61 ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg ttcggccttt
      121 gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct atgagatggg
      181 aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca aaaattcgca
      241 tttctctccc caccacgatt tccagattga atttaacact tgccgatgta ctggataact
      301 tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc aggacaattg
      361 cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt gacccaccgc
      421 catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa ggcagtgatt
      481 tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac cgtgacggac
      541 gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa tgccacttgt
      601 aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa tgctcctttg
      661 catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta
      721 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
      781 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc
      841 ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc ccgcgctcgc
      901 gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt
      961 tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat
     1021 tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc
     1081 attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga tgcaatggtc
     1141 tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg
     1201 gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca acaagttaac
     1261 gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag
     1321 catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt
     1381 gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggttgg
     1441 tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat gctggaagcc
     1501 gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt
     1561 gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa
     1621 tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcgc
     1681 cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc cgacttccag
     1741 ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt
     1801 acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg
     1861 gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat gctgatcgcc
     1921 cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca ctttcagaat
     1981 gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc acacttcctc
     2041 cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg
     2101 gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt cccgtgtcag
     2161 ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc
     2221 atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca cgcgctgttt
     2281 ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc
     2341 caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca gattgccacg
     2401 cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac gacgcttttc
     2461 cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga ggacaatccg
     2521 ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc
     2581 ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa tggtcaggca
     2641 atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc cgcccgttca
     2701 cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa
     2761 ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag ggtatacatg
     2821 gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat ggttgcacca
     2881 aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc
     2941 agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt tcttgcggcc
     3001 tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga
     3061 aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca
     3121 aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataataag
     3181 taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct
     3241 ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta
     3301 aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg
     3361 gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct gcctgcggtc
     3421 ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa
     3481 ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt
     3541 tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta cgttcgacca
     3601 gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc gtatcccatg
     3661 cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt tccggatcgc
     3721 caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg tcgatggtga
     3781 aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct
     3841 gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg
     3901 cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga gtagatgacg
     3961 aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta gcaatgtcct
     4021 gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg
     4081 cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag
     4141 ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact aactcggaat
     4201 tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc ggcaggaaaa
     4261 gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa tcaatgattt
     4321 tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg
     4381 taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga
     4441 ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct
     4501 aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc
     4561 acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct
     4621 ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc
     4681 cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc
     4741 cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa
     4801 aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt
     4861 actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg ccctgccgct
     4921 gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg
     4981 gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact
     5041 ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cacgtctggg
     5101 gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca
     5161 ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gtcatattga
     5221 gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc
     5281 cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca cggcctactt
     5341 taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa
     5401 acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa
     5461 ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt
     5521 taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag tcggcgctgg
     5581 cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc
     5641 acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcaacgga
     5701 ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg gctgagatta
     5761 ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta
     5821 ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc
     5881 tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc
     5941 tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta
     6001 ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc cagtttgctg
     6061 gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg ctccagcaag
     6121 cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg
     6181 atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg ctgaacaatg
     6241 gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag gtgaatccag
     6301 gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga
     6361 ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc cgacgtcgcg
     6421 gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc agtagtttgc
     6481 atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc tattttgatt
     6541 cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag cagtggctgg
     6601 atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt tcctggggac
     6661 agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg
     6721 atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt tttgttgatg
     6781 tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc
     6841 ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat cgctatgtgc
     6901 tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta agcgcactct
     6961 ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc
     7021 aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt
     7081 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
     7141 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg
     7201 tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc atgatgtcat
     7261 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
     7321 cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga tgaccgagat
     7381 cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgtcgccga
     7441 atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc tgcgcctgat
     7501 tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta
     7561 tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac cgcaaagccc
     7621 ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa aagcccagcc
     7681 ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga
     7741 tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaagttgc
     7801 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagatggtac
     7861 tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
     7921 aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag
     7981 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt
     8041 tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca aagccgaccg
     8101 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
     8161 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
     8221 cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc
     8281 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
     8341 tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
     8401 ccccgattac accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac
     8461 gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag
     8521 ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc
     8581 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa aaacgtaccc
     8641 gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca
     8701 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag accgcctgca
     8761 aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc agcgcgagct
     8821 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg tgccttactg
     8881 ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga
     8941 tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga ccagtcgtta
     9001 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg caccatttaa
     9061 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc
     9121 cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc agcgagacct
     9181 gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
     9241 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
     9301 ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
     9361 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg
     9421 tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga
     9481 cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt
     9541 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt
     9601 gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc
     9661 ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct
     9721 taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct
     9781 aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac
     9841 taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg
     9901 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt
     9961 cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga
    10021 gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
    10081 tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggccga
    10141 gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt
    10201 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
    10261 tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt
    10321 cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
    10381 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
    10441 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg
    10501 actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc
    10561 tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc tattggtcgt
    10621 tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt gacgctttcg
    10681 ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg
    10741 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    10801 cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt
    10861 atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    10921 gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg
    10981 gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt gaaatctgtc
    11041 gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac
    11101 gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat
    11161 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    11221 accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac accgtcgcgg
    11281 ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac
    11341 agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat
    11401 tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct
    11461 ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac
    11521 aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt gctgttgctt
    11581 cgtcacggca atataatgag aattattatc attaaaagat gatttgagga gtaagcatat
    11641 ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg
    11701 tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc
    11761 aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt ctgtgctgaa
    11821 acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt
    11881 tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc
    11941 ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt
    12001 gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc cgcgttacgc
    12061 gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt
    12121 gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat
    12181 cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca tgagcgaaga
    12241 agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa
    12301 tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc
    12361 agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa tgagaaagcc
    12421 gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac attgcctgat
    12481 gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg
    12541 catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag ttgacgtaac
    12601 agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc
    12661 ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca
    12721 ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc
    12781 tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg agctggcaga
    12841 cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct
    12901 ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac aatcgccgaa
    12961 tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg
    13021 atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca
    13081 gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc
    13141 atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga
    13201 ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg
    13261 aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc
    13321 ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc
    13381 agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc
    13441 accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatgg
    13501 gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc gacaggagga
    13561 agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca
    13621 gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg
    13681 tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag
    13741 attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa
    13801 ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta
    13861 tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc
    13921 atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca
    13981 gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg
    14041 cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg
    14101 acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa
    14161 tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc
    14221 gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata
    14281 ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt
    14341 ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat
    14401 aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga
    14461 agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt
    14521 agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat
    14581 gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc
    14641 tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa
    14701 tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg
    14761 cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg ggttgctggc
    14821 aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg
    14881 agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc
    14941 gttgattatt atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc
    15001 gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga
    15061 aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc
    15121 ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac
    15181 gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca
    15241 ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat atcaaccttg
    15301 tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta
    15361 atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt
    15421 gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc
    15481 aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt
    15541 acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg
    15601 gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat
    15661 gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat
    15721 cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt
    15781 caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc
    15841 ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat
    15901 gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc
    15961 aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata
    16021 accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt
    16081 tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta gccgcaaaat
    16141 ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg acatgaacct
    16201 cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca ttcatggcca
    16261 tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt ggtgcttcca
    16321 gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga tcggttgcca
    16381 caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg gtggaaccga
    16441 ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat
    16501 ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatgc
    16561 aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa cgccccagat
    16621 gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga
    16681 cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca gaagtgttgg
    16741 cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat tcttcaacag
    16801 ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt gttttttccg
    16861 gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag
    16921 cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt
    16981 cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac
    17041 gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg
    17101 gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct
    17161 taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat tcgctgttat
    17221 cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga
    17281 attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca
    17341 ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt
    17401 ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    17461 ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    17521 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg ctggatacta
    17581 caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat tcgaacctgc
    17641 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg tggtgggtga
    17701 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
    17761 tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg tgcaggatga
    17821 ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt cacgttttac
    17881 taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
    17941 taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc tttcggtttg
    18001 tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag cgcaacaaaa
    18061 atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga aataatgcga
    18121 cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct gtttagcgcc
    18181 atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt ttgcggtgtc
    18241 acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct ttggtgactt
    18301 cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt tacccttgtt
    18361 gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat cagggtaatt
    18421 tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat cctgcaccag
    18481 cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg cgccagcatt
    18541 cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt gatcgctgct
    18601 ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca ggatctgctt
    18661 ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac cacgcaggga
    18721 atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa gctggctgtg
    18781 agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg agctgacact
    18841 actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca gtaacgacag
    18901 actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa ccagtaccgc
    18961 acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt tttctttacc
    19021 gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt acatcttaac
    19081 ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac gttcgtccgc
    19141 gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg catgtgcatc
    19201 cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt aaacgatgtt
    19261 gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca tgttgccgtt
    19321 gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc cgtcattgct
    19381 ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa tcatcagccc
    19441 tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt aattattatc
    19501 acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga agacgcgctt
    19561 tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga gaagagctgt
    19621 agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac gtggacgaca
    19681 gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc ccaccattgg
    19741 agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg ttctgcgaac
    19801 cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa acctgcggac
    19861 gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag gtcggttcgg
    19921 tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa tccattacgt
    19981 acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg tctggcgaga
    20041 atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg ccatttgcca
    20101 gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc agtttagaac
    20161 cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg acaaactggt
    20221 tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg ttggtctgaa
    20281 caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt tcaaacactt
    20341 catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg tacgagttct
    20401 gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca acattgtaag
    20461 aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc agtgcggacc
    20521 atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga gcgcgatcta
    20581 acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca atatcttcag
    20641 gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga ccggaatcta
    20701 caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac agtatgagaa
    20761 aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga ccgatggccc
    20821 acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga accccgtggc
    20881 aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt ttgaagtcca
    20941 atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg atcttcgcaa
    21001 gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca ggtttgatat
    21061 ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg cctgcatagg
    21121 acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca tagttattga
    21181 tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc gaagcattgt
    21241 tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc tcaccgaaaa
    21301 tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct gcttttgccg
    21361 ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca gctgctgcct
    21421 tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct gctgccgctt
    21481 tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc ttggcttttt
    21541 cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct gccgcttccg
    21601 ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg gctgcggctt
    21661 tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc gctgcttctt
    21721 ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc ttctgcttta
    21781 actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc gctaaccgct
    21841 ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc agttcttcag
    21901 cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt gattcctggc
    21961 tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca gcgtcgatgg
    22021 acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa ctccagatca
    22081 gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc ttgagcttgt
    22141 cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc tgttacccgc
    22201 tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa accaaccgat
    22261 ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta aggcacatct
    22321 tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct ggacacttcc
    22381 gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac cacggtgtac
    22441 tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct caccgcctgt
    22501 gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg cgctgtcgcc
    22561 ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt gatttcggac
    22621 ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt gttgctctcg
    22681 ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa gttgtcgtaa
    22741 ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac cgccggaatt
    22801 gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg cgcaaccatt
    22861 tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat cccccagacc
    22921 gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa cggaatgtgc
    22981 gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga cgccccttcc
    23041 actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc tttgaacccg
    23101 ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact ctcttgatag
    23161 aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc ttcacgcgcc
    23221 gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat tgaaaaacca
    23281 atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa atcaaggata
    23341 ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca cgaggcttca
    23401 ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa gtattctcgg
    23461 cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt atttcagtct
    23521 gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc attttacgta
    23581 ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga tgacgcagca
    23641 tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc acaccaccgg
    23701 catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta ttcactttac
    23761 atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg cgcgcgattg
    23821 aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta gagcggccca
    23881 taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga caatgagaac
    23941 aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg gacgaaagcc
    24001 cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat gccagatttc
    24061 cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc ctgctaacag
    24121 aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg catacaaggt
    24181 cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc gacgtgttca
    24241 agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag aatccatgcg
    24301 aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt tcaatatctt
    24361 ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc agaatgatcg
    24421 gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg gaagttgcat
    24481 cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac gggaacattg
    24541 cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca aacgcccacg
    24601 cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca cccagtgccg
    24661 gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca gcttcacgaa
    24721 ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat ttcacgacat
    24781 aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc agtgtcacca
    24841 gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg gtacgcattt
    24901 gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct gccagcaggc
    24961 cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga tattcatcaa
    25021 cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc agcggcggaa
    25081 cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt tcttcaatct
    25141 tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg aggatcatcg
    25201 ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca aagagtgcgc
    25261 cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg gagttaatca
    25321 taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc acccccatgt
    25381 cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc caccagataa
    25441 aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc ggctgagtag
    25501 tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg ccgaggcgtg
    25561 caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg ccgcaaatca
    25621 gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca gctgtcggca
    25681 gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag cccgcttcta
    25741 cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat tttttctcgc
    25801 caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc agcaggaagc
    25861 cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg atggagtctt
    25921 tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta tagcgtttca
    25981 gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga accgtctggt
    26041 cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg ttacgaatgc
    26101 gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc acggaacgcg
    26161 ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc tcctcttcct
    26221 gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc cactcggctt
    26281 caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat tcatcaccca
    26341 gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca aaggagcgtt
    26401 tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg aggatgaaca
    26461 tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga gcaaccgggt
    26521 taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag ttgaaatcgg
    26581 acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg gacaggtttg
    26641 aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga cgatcccaac
    26701 cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga ccttcgattg
    26761 ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag ttagtcccga
    26821 actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata ccaaacaact
    26881 tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg tagaccgttt
    26941 ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg aagtggtaca
    27001 tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc atgactcctt
    27061 gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac gcatgcccca
    27121 atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa acggcgtaaa
    27181 gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata ttacgccgca
    27241 aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga cggtgatcaa
    27301 tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat ctctttttga
    27361 tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta tgttaatcat
    27421 gtttcaacaa ggtaacttta gtgatattta tgtttaaaat gtgtgaatta taagactgaa
    27481 attagtttcg gttggcaatt aaagataaaa agagacgaag caaaaacagc acggttgacc
    27541 ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta ttaataaagg
    27601 cataaatctc cctcccgccg atattaacat ttaattaact tataattata ttataattaa
    27661 ctcacccgtg gttattattt tgttaaaaat aaagccacat atacgtagct tgagagtgaa
    27721 gataaacaca aaattacctg atgcgctacg cttgccagtg tctacatcat ctctgcaata
    27781 tattgaattt gcctgctttt gtagaccgaa taaagcgttc atgccgtatc cggcatgaac
    27841 aaagcgccct ttgtcaacaa tctggccgcg catatgtggc ttcggtttga ttgctattca
    27901 ggcaagccgg taactgaacg tccgtgactg gccgggtaaa aaaggccccg atagatttga
    27961 accttgattt tcctcggtgg taatgtgttc atccatcatc gtttctgagc aagaaatcac
    28021 ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat tcagccggat taaacagccg
    28081 taaaatcact tcttgtcggt cttcagcttt cttaagtgcg cttatcaggc atcccactgg
    28141 gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt tgagcttcat
    28201 cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc gcgcctgctg
    28261 agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc gacaagaaag
    28321 cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc gcctaggcct
    28381 taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa tggcaaaggt
    28441 tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga cagccatgcc
    28501 gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa ccggcgcttc
    28561 tttccagctt tcttgctgcc agttgttcat tgcactgtcg ttcacggggc gcgttagcga
    28621 accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga tcaggacgcg
    28681 aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc gcctgttgtt
    28741 atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc ttgcgctgcg
    28801 ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc tctgccaggc
    28861 ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc actcttcttt
    28921 tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt gcaacacccg
    28981 gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct cattgagcgc
    29041 aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg caatcacgtt
    29101 gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga caatctggtt
    29161 gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta tttgccgatc
    29221 gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag gctgaccgcg
    29281 atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga tcacttcttc
    29341 ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct gcggcatgtt
    29401 gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt cagccagttc
    29461 gaagcgggcg acgatttcgc gatgaacttt gtcacgacag cagcagccga tgctgtcgtg
    29521 ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt ggtatccaaa
    29581 acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct tattttcaat
    29641 acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat gcacgcgcat
    29701 atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct gcgcttcgat
    29761 cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat aaatttcgcg
    29821 cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat gcccattcgg
    29881 cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac tgtcgaggcg
    29941 tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac ccaacggcat
    30001 cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg ttttatccgt
    30061 accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa atccattgta
    30121 gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta ttttcatcgg
    30181 ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga tggattccgc
    30241 agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact tgccggcttc
    30301 taccagtttc ttcaagcggt ctttgttttc cggtttcacc gcgaaataat cttcgaggat
    30361 cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca gtcggcacag
    30421 gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga aataccactc
    30481 ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca tcgtcgtttc
    30541 ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac cgcgtgacgc
    30601 cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc accaaaccag
    30661 ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag cgagaaaatg
    30721 ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc cgttaccata
    30781 gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat cgggatcgcc
    30841 ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc aatttcaaac
    30901 tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc cgaaaccatt
    30961 ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc gcccaggcag
    31021 aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat aaaaccgaga
    31081 atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc ggtaagcgag
    31141 ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc cgctcccaat
    31201 gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact gctaagacgc
    31261 aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac gaccgcgccg
    31321 agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg cgctaacgcc
    31381 ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat caaaattccg
    31441 agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc attaaacaga
    31501 tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt cccccccgcg
    31561 acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag ctccgttttg
    31621 acgcttttca ccggttgcgt atcttgctgt aaggtacgcg tctcatcgcc acgctcgagg
    31681 gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac aggcacagaa
    31741 agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc cgcaaaaata
    31801 caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc gttagcacct
    31861 tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag atactccgca
    31921 gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat agttggcgcg
    31981 ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga gagcaattca
    32041 tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag gcgcgtggtc
    32101 agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat cgccagcaac
    32161 accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg ctcgctgagt
    32221 gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg cacagccaac
    32281 ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt cagaaattgc
    32341 tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg aatggcctct
    32401 tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag agtcgtcagg
    32461 ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg acaaaacaat
    32521 accattgcgt aatcaatttc acttgattga aattatttgc attttatttg tattgtttac
    32581 aggatgagtt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta aataatggga
    32641 cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc gcgtggcgag
    32701 cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt tggcgtcagc
    32761 cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct cgaaagcatt
    32821 cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc
    32881 agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt tctgatattt
    32941 gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt gcaggatcgt
    33001 gtttggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc
    33061 tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa ttcgaaatat
    33121 cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga aatcattcct
    33181 ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa gccgattctt
    33241 gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct
    33301 tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc gcgataagca
    33361 caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc gccattcctg
    33421 tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa cagttttcag
    33481 tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt ccagagcagc
    33541 gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt ggcccatacg
    33601 tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg taacatgctc
    33661 tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga tcatcacgat
    33721 cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt tagagccagg
    33781 gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat ccgtggtctg
    33841 tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt tacccggttt
    33901 gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa cgcctgggca
    33961 gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg tcagcatatc
    34021 cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat cgatagcttc
    34081 cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg aagcgccagt
    34141 agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg tagtgccgcc
    34201 tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct gttctgagtg
    34261 gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt tatcaattaa
    34321 aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc cgtcagacct
    34381 tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag ttcggcgttg
    34441 ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac cgcgccgatg
    34501 ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac cagaacggct
    34561 ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc tttggttgcg
    34621 ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac tatgtccatc
    34681 gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc cagcgcaacg
    34741 tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga ctggtcacgc
    34801 atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag tttgccgtcg
    34861 aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat cagcgccagg
    34921 tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg ctgaaccagt
    34981 ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat cgggccagtc
    35041 agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac tttttcgatt
    35101 tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg gtctacaacg
    35161 gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat ctggttaacc
    35221 ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca gttttctgca
    35281 aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt accgcggcca
    35341 ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga agcggcttct
    35401 tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc atagcgggca
    35461 aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc
    35521 gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg caggtgaagc
    35581 ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc tttgatcgtt
    35641 accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga cagcgccagg
    35701 tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc tttgatagcg
    35761 tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg
    35821 aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac cgtcagcttg
    35881 ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc catgccgagg
    35941 gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac cgccatgctg
    36001 acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt cacttccggg
    36061 taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc cagacggatg
    36121 ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat cggcttcatg
    36181 ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag cagacgctct
    36241 gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc agccagagcc
    36301 ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc tttcgccaga
    36361 tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt aatggcgctg
    36421 gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc atcgttgttt
    36481 tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc ttcagatttg
    36541 gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat ctgacgagac
    36601 gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctgc tgatgccggt
    36661 acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg tacgactgcg
    36721 tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc aggcaggtca
    36781 gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg ttcagcgcgt
    36841 cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc gccggagagg
    36901 cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact tcacggaaat
    36961 gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac cagacaaaat
    37021 ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg aacgggtaga
    37081 gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc tgttccagca
    37141 ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc ggatcaagct
    37201 cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa
    37261 ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga cgacgcatcc
    37321 cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac ggtacgcaaa
    37381 cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg gaggagtgct
    37441 ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac atccggcccc
    37501 atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg ttggcgaagt
    37561 caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca taaccatatt
    37621 caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac gcgccctgac
    37681 cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg tggatcaccg
    37741 cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga accggaatgc
    37801 cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg aacagtttct
    37861 caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac tgcatttcaa
    37921 ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc atctcaactt
    37981 tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca
    38041 tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca tcgcggtaca
    38101 ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc agcttgtcag
    38161 cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt ggctgggttg
    38221 cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg tcgatgaaga
    38281 catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag gcaacggctt
    38341 ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg atatcagtac
    38401 agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc tggttgttga
    38461 taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg ttcagagttt
    38521 cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt
    38581 tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg ataacgaccg
    38641 ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc aggccgccat
    38701 cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg ccgaggtgtt
    38761 ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca
    38821 ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt
    38881 tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg ccttccagcg
    38941 agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct tcagcggcgg
    39001 tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga ccagactcga
    39061 tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac tcggcaccaa
    39121 tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc atggtttctt
    39181 tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg gtcagatcgt
    39241 ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc ggatctagat
    39301 tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact ttaacctgct
    39361 tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc
    39421 ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc gtaccaggta
    39481 actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt aagaagtctt
    39541 catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag tccaaccagg
    39601 ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc
    39661 gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc tcttgtctac
    39721 gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga
    39781 tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc gggtaatgac
    39841 ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat gcaacgcttg
    39901 cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac ttgatatggc
    39961 cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc atgatgctgt
    40021 gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca gtctcggtat
    40081 cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg ataaacttat
    40141 cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga atacattcat
    40201 acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga gctggcggat
    40261 tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag aattgtccca
    40321 tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc acaatcttct
    40381 tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc ttgccgttca
    40441 tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac gacaggctgg
    40501 gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga ccttcttccg
    40561 cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta tagcgataaa
    40621 ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc ttcggcggga
    40681 atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat tccgactcag
    40741 gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag tcgaagcggc
    40801 tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca tacgccgttt
    40861 ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg ctggaagtgt
    40921 catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca agccctttcg
    40981 ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct tgcagcgctt
    41041 tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc aggcgatcca
    41101 gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg gcgatagact
    41161 cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc gagttgccgc
    41221 ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca aacagtcccg
    41281 gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta actttggtcg
    41341 gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct ttcaccggat
    41401 cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat tcgagaactt
    41461 ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca tcacagccgc
    41521 gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc gccaggtctt
    41581 tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc agataaccgc
    41641 cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg gtcgggtgga
    41701 attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg ccgacaccgt
    41761 cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct ccgccagtcg
    41821 ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg cacagtgcgg
    41881 tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac cactcggaga
    41941 aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt gcgtgaccgg
    42001 tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt ttcgactgac
    42061 cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc aggcccatgt
    42121 gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc gcgtcctggt
    42181 caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag ttatcttcat
    42241 gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg
    42301 ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc agcgccgcgc
    42361 gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact ggcaatttca
    42421 tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg caacgacaat
    42481 caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca acacctgcca
    42541 catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga acactttggt
    42601 gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac tggtggcgaa
    42661 aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc gaacgaggat
    42721 gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac gaggactcct
    42781 gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg tttacccgct
    42841 tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg
    42901 tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa gctgcccata
    42961 atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag aagccactgc
    43021 aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat ggacgctatc
    43081 gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg ttttttcaca
    43141 tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga ctacgggcac
    43201 agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg
    43261 gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa
    43321 acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga cctggcggca
    43381 gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg ggttttatcc
    43441 tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat ctaatttgac
    43501 aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc gtaaaagtta
    43561 tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat tgaccaattc
    43621 attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa gtacttgatt
    43681 cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct ctgtacccag
    43741 gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc caggttgatg
    43801 tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg agaccttaaa
    43861 tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa ctggatgtgc
    43921 tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca aaaggtgtgt
    43981 tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt acttttattg
    44041 atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt
    44101 ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact caggaacagt
    44161 atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag attacccgtc
    44221 tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt attaccggcg
    44281 cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat cgtgaaattg
    44341 ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac aagtattcca
    44401 ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac ttcctgaata
    44461 tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa cgtgctatgg
    44521 accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc accgtgcgta
    44581 ccgctggctc ttcgggggcg aacccgtttg cctgtatcgc agcaggtatt gcttcactgt
    44641 ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa gaaattagct
    44701 ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc
    44761 tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg
    44821 aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg gaagtggcta
    44881 tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa ctgtacccga
    44941 acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct tccatgttca
    45001 ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc gaaatgcaca
    45061 gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa aaacgcgact
    45121 ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt taacccgccg
    45181 ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca
    45241 ctatcggaca ttcatatatt ctaaaaaaaa atacatcggt ataaattata aacaaataga
    45301 tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa tatagcgcac
    45361 ccctataatt cccaatactt tattcatcaa acatttaatt cataccatca attaattact
    45421 tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc gacaatcaaa
    45481 taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactca gttatgttta
    45541 aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca cctgtttatg
    45601 cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa gcaccgtgtg
    45661 aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc accagccaca
    45721 ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gctcttagtc aactgtgatc
    45781 tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc acctttgata
    45841 gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg ggtgaagcca
    45901 ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta ggcaccgcta
    45961 ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc tttgcctgga
    46021 tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg aatgcaacct
    46081 acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt tcataaaata
    46141 tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct gcctggtcgt
    46201 ggcgatgcct tgcgcattgg cggtcgaatt caatctgaat gttctcgata aatcaatgcg
    46261 cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg gtgagtattt
    46321 tgttagcgtt gcggtaaata ataaccaaat cagtaacgag caaaagatta actggcacaa
    46381 aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa
    46441 acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta gctcccgacc
    46501 tgaaatgctc ttcaatttcg atcaagccaa tcagcaactg aatattagta ttccgcaagc
    46561 ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag aaggtgtcgc
    46621 cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg atggcagcag
    46681 cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat ggcgcttacg
    46741 cagtgattat cagttgaatc atactgatag cgatgataac catgaacagt caggcgaaat
    46801 atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa ccctcggcga
    46861 aacggatttt agtcccaata ttttcgacgg tttttcttat accggcgcgg cactggcaag
    46921 tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta gcggtattgc
    46981 acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc agaaaaaagt
    47041 cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca cactggatgt
    47101 caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcagcag catcgacgcc
    47161 cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc cacggccctc
    47221 catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct gggggatgct
    47281 gtcaaacacc tcgctgtaca gcggcctgct gctttctggt gatgactacc attctgccgc
    47341 aatgggtatt gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc
    47401 cagtagccat tttgatactc agcaggacga gcggggctta agctaccgtt ttaattacag
    47461 caaacaagtg gatgccacta acagcacgat ttccctcgcc gcttatcgtt tctccgatcg
    47521 tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg atgcgcagga
    47581 cgaaaaacag acgatcagct tatctgtggg ccaaccgatt accccactaa acctcaatct
    47641 ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat
    47701 cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt ctacgtcatt
    47761 caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt cgatttcgct
    47821 ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac
    47881 acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg
    47941 actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag
    48001 ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag
    48061 cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag
    48121 ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca
    48181 gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta
    48241 ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga
    48301 aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg
    48361 ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg
    48421 agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca
    48481 tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca
    48541 gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc
    48601 ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg
    48661 gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac
    48721 gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc
    48781 gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg
    48841 ccgccgatac aacgtctgga accgaaagca acaacacagg tacggatagt aaagcaagcc
    48901 tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt
    48961 cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt
    49021 atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg
    49081 cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat
    49141 ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg
    49201 atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt
    49261 tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga
    49321 caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg
    49381 tcatctgtct gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc tactttggtt
    49441 catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg
    49501 ccaaactcgg cgataagatc tgggaatctg acgatattaa aataagatct gggaatctga
    49561 cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg aaagcgaaca
    49621 tgtctacgta tgggtaaacc cctacccggg ggtgcaggat cgttattatc aactcggagt
    49681 aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca
    49741 gtgtatcgac agtaagaaca ttgacattta tacccctgat gggatggcag aacaaaattt
    49801 gctcgggtga tcccgctaat attcacatgt cgcgaacctt tctcgctcgc atgcggttat
    49861 acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg
    49921 tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat
    49981 atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt
    50041 caccggaaac acagatgatt gattttggta aatttaattt gctggatatt cgtcgccaca
    50101 caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg
    50161 gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat
    50221 tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct
    50281 tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac gaaaaaacct
    50341 atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc ttcgatacag
    50401 tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca gccggaaagg
    50461 gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct
    50521 agcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag ggagcgaagg
    50581 atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc tattggtttc
    50641 ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg gatcatcttc
    50701 agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc gcaggccatt
    50761 cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgccaac
    50821 tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat cgtgggttgg
    50881 ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc ttctccaggc
    50941 ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggtggcg
    51001 tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt cacccgcatg
    51061 atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc gccagtgagc
    51121 attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg atgcatgctg
    51181 cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt
    51241 cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat ggcaattggc
    51301 tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg cccgctaccg
    51361 caaatcctgc tgtcgatttt tgctctgctg gctatttgtg caggtatggc gtggggactg
    51421 acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc cggcgggctt
    51481 gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat tatggcgatg
    51541 caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg gtttatttca
    51601 acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta aaacgggcgc
    51661 gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc gccatcacga
    51721 tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac ctgcccccgc
    51781 gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc cagttgcgcc
    51841 gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag gatctccacc
    51901 cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc tgcaaactga
    51961 cggttacgaa agcgcggcga caataaccgt tcctttacca cctccggcgt cagattcgga
    52021 tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc
    52081 aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt
    52141 acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg ccagacgccg
    52201 taaagctgat tatggctgta gagcgttaaa ccgttgggaa aatgagttaa caacgcctta
    52261 ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata agtttttaac
    52321 tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta
    52381 tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta
    52441 ttaagcgggg ttatgctgcg acaacaatcc cctttttggc aaatgtagct cgtaatcggc
    52501 gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc gccgcgaccg
    52561 ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctcc agcgtttgcg
    52621 ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc accagcgagc
    52681 cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct
    52741 tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgcc ggatcgcaag
    52801 cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca
    52861 acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca atcgttgcca
    52921 gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc cggcggcagc tgcatggcgc
    52981 tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt
    53041 ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga aatccacaag
    53101 caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca
    53161 gatcggcgtt caggtcaatt ttcattgtgc agccgccacg ctaattgttc aaaataacgt
    53221 tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc
    53281 tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgt
    53341 ggataaccac cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc gttatgcggc
    53401 acctgcacca cgcccggtaa caaaccgtga gataacagtt cgcgatcggt gatgcgtttt
    53461 aaaatttgcc cctgtaaacg atagcccatt cggttacttt gcgagctaag ctgccagggc
    53521 gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag
    53581 gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc
    53641 ttcgatttgc caatcgggag tcagtcacca tccttcagta aacggccttc cagcccgcca
    53701 atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc
    53761 gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct
    53821 gctttcatcg gcaatcgcca gccggtccag acggcgttat catccaaccg cgcttcgcaa
    53881 cctgcacccg tcagggcaaa ccacccatca gtttcgaact caactgttaa ctgaccgagc
    53941 gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgcagc
    54001 gcaggcatat ccagtgcgcc acagtggctg atacccgact ggcaaaaacc gtgacgaccg
    54061 ccatcctgca cagtggtgta catgcccgca cgaataatct tcagcatatt ccctccttct
    54121 gcggtacaaa tcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat
    54181 caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat
    54241 aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt
    54301 cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt
    54361 gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt
    54421 cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa
    54481 ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc tcccaccagc
    54541 gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa atcaccgtga
    54601 tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgcgcca
    54661 gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg
    54721 ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt
    54781 tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac
    54841 tcgctcaatg cgcgaatacc accgcgttca gtggcatggt gacctgcagc ataaaaatgc
    54901 aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca
    54961 tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggcg
    55021 acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc
    55081 gcttcaatcc aggaggctag ttccattccc ggcacaggca tggtcagctc tccccacggc
    55141 accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag ctgtgcgtta
    55201 ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt gatatcattc
    55261 gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga ctctcctttc
    55321 cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc
    55381 aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt gccttccacc
    55441 tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt
    55501 tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa
    55561 catagcgtta catcgcctgt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc
    55621 cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca
    55681 atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac
    55741 acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt attatgaata
    55801 aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc tgggaatatt
    55861 ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca
    55921 acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa
    55981 tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg atgctggggg
    56041 cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat ccgtcattcc
    56101 tctatctgtc cctggcgatt atcgtctgcg gctatggcct gtttaaatct aacgtaagct
    56161 gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga
    56221 tgtatgcggc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt tacgcccagg
    56281 aagagtacag ctgggcgatg ggctttggcc tggcggcggt tggaatgatc gcgggtctgg
    56341 tcattttctt atgtggcaat cgtcatttca ctcatacccg cggcgttaac aaaaaagtac
    56401 tgcgtgcgac aaactttctc ctgccgaact ggggatggct gctggttctg ctggtggcaa
    56461 cgcctgcact gattaccgtg ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg
    56521 cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa aaccagaaac
    56581 agcggaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg ttctgggcct
    56641 tcgcacaaca gggcggtagc tcgattagcc tttatatcga ccgcttcgtt aaccgcgata
    56701 tgtttggtta taccgttccg accgcgatgt tccagtcgat taatgccttc gcagttatgc
    56761 tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc
    56821 gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc tgcattctga
    56881 ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg atggtgttag
    56941 gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta
    57001 cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg ctgctttctg
    57061 gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg
    57121 atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta
    57181 cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat caggcgctga
    57241 aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat gcctgatgcg
    57301 acgcttgacg cgtcttatca ggcctacgta tttcctgcaa tttattgaat ttgcacaaat
    57361 ttgtaggctg gattaggcgt tcacgccgca tccggcatct gggctcgatt gcctgacgcg
    57421 tctgttattt ccccttccgc gccgcctcat acgctgccaa cgtttgtact ctcgcctctt
    57481 tgtgctcgac tatcggttgc ggataatcca gcttcacacc tgctttctgc gcccacttcc
    57541 acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat cgacgaataa
    57601 actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata cgaaaatacg
    57661 gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta gctgccaaat
    57721 caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat
    57781 ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag ccagtgctgt
    57841 taagctgacg catagcggca tcaataatcg ggtatcccgt tttgcctttc tgccaggcct
    57901 gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata aatggacgat
    57961 gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct
    58021 cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc
    58081 gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa gctggcggac aaacggctgg
    58141 tgccttccac tgccggaaaa tctcgttgtt gctcatactc tccagcaccg ttctcgcaaa
    58201 actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag
    58261 actgacgagg ataattcagc gtaatggatg gcgcgggctt tatcgatccg ctactacgaa
    58321 cttttggtgc agcgacgcac tccggcatcc cttcccgcag ccgtttcagc caggcattct
    58381 taaaaggcgt aaagactttg tacatctcgt gattgccggt catcaccgcc cccggcggca
    58441 ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacgtcca
    58501 catcccgcgc tcgctcattc acctcatact gatagttata aaaaagatga gtaacgctgt
    58561 tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga
    58621 acaataaagg aatacctttt tccgccagcg cgatttgtag cccattcagt tgagcattga
    58681 taagttcagc ctgacgcggc gacatgttat gcgtctccca cttgcgtggt gtagcgatat
    58741 acagcgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt
    58801 gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc ctgaatggcg
    58861 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
    58921 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg
    58981 gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa tatgcgggcg
    59041 aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
    59101 aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat tttgccactg
    59161 atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat gggccagaaa
    59221 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
    59281 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
    59341 tagattcatt actgctcctg aacggtatac aggagtaagt gtaggcgttg gtaaatgatg
    59401 tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc gcaagcaact
    59461 aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcact atttccaaaa
    59521 cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc aaaaccggta
    59581 tagcccggta ttattcacaa catcccagtc tgcaccttaa gggcgactgg ctgaaagagg
    59641 ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggggc ctgaccatca
    59701 tcgctgacag tgacgaggtg caggaactgc gggatgggat gtttagtgtg ctgaacgaaa
    59761 gttaaatatt aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat
    59821 tataatggat actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga
    59881 gtacttaacc tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa
    59941 tcatatgatg gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt
    60001 ttaaatttag cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca
    60061 tattcatcta atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc
    60121 catgttctat atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat
    60181 aatgtattga gattgttcat tattatgtct cccacgggaa aaatatatgt ctaacacttc
    60241 caccatgttg atgaacttgt aaatgtggta aatcaccatc aggatttcct ggcccatgac
    60301 ctgacactct atatgaaggt tttgttacag aaggatcttt tatatcatcc caatgatatg
    60361 aacctgcttt tgttttattt tcagtaagat attccattaa gttcttagtg ctggggcctg
    60421 actcacctgt catattttta taattatgac ctaaatatct atccataaac aattgttcag
    60481 cgtcacttct agacctaaca gtaactttgg tttcactact atctaataaa cgattcgctt
    60541 tttggttact aacagcacat ggagttaatc caagtggatc agcatatttt agaggattag
    60601 gtgcataact gtagagattt aaaccgcctc tcaaccctat cgggtcagtt accatgaacc
    60661 gcccgacatc cggatcatag tacctgtgca gattgtagtg cagccccgtt tccctgtcga
    60721 ggtactgtcc ctggtagcgc aggttctgct ccaccccgcc gtggtccttc tcctgtatcg
    60781 tgttacccca gacctgatac cccgtctccc acacgatttt cccgtcgctg tccgtcatct
    60841 cttccggcgc gccgttcaca tccgtgtgaa aatacagtat ccggttcggg ccctctttgc
    60901 ccgccttatc cacccgcgcc agcggttcat aactcccctg gtccctgtag atgtacaggc
    60961 tgcctgttcc ctccggcctg ctctcctgcg ccagccgcat tccgtcccat aaaaattttg
    61021 tccggttgta cggcttaccg tcagggctga tgtgctgttt ttccgtccgc ctccccagtg
    61081 catcgtacac ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc cggtgttccg
    61141 catcataacg gtaactctgc gttccgctcc ggccctcctt ttccaccgca cggccaaatt
    61201 cgtcataacg gtagtgcaca ccacggtaag acatcagccg gttgaacggc accacgctgt
    61261 tttctgccgt ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc gcgtccacct
    61321 ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc cccagcgggt
    61381 cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg ccgccctgag
    61441 ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc
    61501 cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga taacgtgtgc
    61561 gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc agccggtact
    61621 gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat
    61681 accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt
    61741 ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac acgccgttca
    61801 ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat
    61861 gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac agacggatgc
    61921 acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg tagtacagat
    61981 tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg
    62041 ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag tgcccctgct
    62101 caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg
    62161 cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt tcgtctgcgc
    62221 tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga ccaaacattc
    62281 cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg
    62341 gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg gcggcattga
    62401 ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg acactggcag
    62461 caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc gccacggttg
    62521 ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt aacggaccgc
    62581 caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc accctggcct
    62641 cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg
    62701 cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac agattactga
    62761 tgctgccaat aaccgcattc actccctgaa aggcggtttc cagcataccg cccggattca
    62821 gcaggttaag ggccgttccc agggggtcat ccagaaaggc ggcttttttt tgtgcgagaa
    62881 actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg
    62941 atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa tgtgtcacgc
    63001 catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga gcccgggaca
    63061 tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc atttccagat
    63121 attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct tcttcctgtt
    63181 ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg
    63241 cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg atgtaacctt
    63301 caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg accttttctg
    63361 tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg acggccgtgc
    63421 accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat
    63481 caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc ataaacgacg
    63541 tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg tcccgttccc
    63601 tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc tttttaattt
    63661 cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag gtgaaggcca
    63721 tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc tgctcgttac
    63781 cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt ccgtcataat
    63841 gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt tttaatcatg
    63901 aatgtaatac ccatcaccgc tttacagcac actttatctt tccagtcata atcactaata
    63961 atattaagca attagcaata tttacaacga ccagagcgga tacatagtta tgtatttaac
    64021 aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct accgcctcca
    64081 tataaaaatg gacaccttat aaaattgtat cttgcat
//
LOCUS       DAJTRW010000011        60420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRW010000011 DAJTRW010000000
VERSION     DAJTRW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..323
                     /locus_tag="OO326_000768"
     CDS             <1..323
                     /locus_tag="OO326_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5965263.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            436..1230
                     /gene="panB"
                     /locus_tag="OO326_000769"
     CDS             436..1230
                     /gene="panB"
                     /locus_tag="OO326_000769"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5965264.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1242..2093
                     /gene="panC"
                     /locus_tag="OO326_000770"
     CDS             1242..2093
                     /gene="panC"
                     /locus_tag="OO326_000770"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5965265.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO326_000771"
     CDS             complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO326_000771"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5965266.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3343..3723
                     /gene="panD"
                     /locus_tag="OO326_000772"
     CDS             3343..3723
                     /gene="panD"
                     /locus_tag="OO326_000772"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5965267.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3727..4956)
                     /locus_tag="OO326_000773"
     CDS             complement(3727..4956)
                     /locus_tag="OO326_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5965268.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5020..5460)
                     /locus_tag="OO326_000774"
     CDS             complement(5020..5460)
                     /locus_tag="OO326_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5965269.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5565..6335)
                     /locus_tag="OO326_000775"
     CDS             complement(5565..6335)
                     /locus_tag="OO326_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5965270.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6332..7258)
                     /locus_tag="OO326_000776"
     CDS             complement(6332..7258)
                     /locus_tag="OO326_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5965271.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7367..8029
                     /gene="can"
                     /locus_tag="OO326_000777"
     CDS             7367..8029
                     /gene="can"
                     /locus_tag="OO326_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5965272.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO326_000778"
     CDS             complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO326_000778"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5965273.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVIQLDE"
     gene            8812..11202
                     /gene="gcd"
                     /locus_tag="OO326_000779"
     CDS             8812..11202
                     /gene="gcd"
                     /locus_tag="OO326_000779"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5965274.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO326_000780"
     CDS             complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO326_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5965275.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12965..13312
                     /locus_tag="OO326_000781"
     CDS             12965..13312
                     /locus_tag="OO326_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5965276.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13418..14284
                     /gene="speE"
                     /locus_tag="OO326_000782"
     CDS             13418..14284
                     /gene="speE"
                     /locus_tag="OO326_000782"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5965277.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14300..15094
                     /gene="speD"
                     /locus_tag="OO326_000783"
     CDS             14300..15094
                     /gene="speD"
                     /locus_tag="OO326_000783"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5965278.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15132..15494)
                     /locus_tag="OO326_000784"
     CDS             complement(15132..15494)
                     /locus_tag="OO326_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5965279.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO326_000785"
     CDS             complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO326_000785"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5965280.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18621..20474
                     /locus_tag="OO326_000786"
                     /pseudo
     CDS             18621..20474
                     /locus_tag="OO326_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO326_000787"
     CDS             complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO326_000787"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5965281.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO326_000788"
     CDS             complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO326_000788"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5965282.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO326_000789"
     CDS             complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO326_000789"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5965283.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO326_000790"
     CDS             complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO326_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5965284.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28318..29691
                     /gene="aroP"
                     /locus_tag="OO326_000791"
     CDS             28318..29691
                     /gene="aroP"
                     /locus_tag="OO326_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5965285.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO326_000792"
     CDS             complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO326_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5965286.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO326_000793"
     CDS             complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO326_000793"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5965287.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31224..32117
                     /gene="nadC"
                     /locus_tag="OO326_000794"
     CDS             31224..32117
                     /gene="nadC"
                     /locus_tag="OO326_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5965288.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32320..32760
                     /gene="ppdD"
                     /locus_tag="OO326_000795"
     CDS             32320..32760
                     /gene="ppdD"
                     /locus_tag="OO326_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5965289.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32770..33917
                     /gene="tadA"
                     /locus_tag="OO326_000796"
                     /pseudo
     CDS             32770..33917
                     /gene="tadA"
                     /locus_tag="OO326_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33907..35109
                     /gene="hofC"
                     /locus_tag="OO326_000797"
     CDS             33907..35109
                     /gene="hofC"
                     /locus_tag="OO326_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5965290.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO326_000798"
     CDS             complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO326_000798"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5965291.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36349..36387)
                     /locus_tag="OO326_000799"
     CDS             complement(36349..36387)
                     /locus_tag="OO326_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965292.1"
                     /translation="MIIKTLKMSARK"
     gene            36412..37032
                     /gene="coaE"
                     /locus_tag="OO326_000800"
     CDS             36412..37032
                     /gene="coaE"
                     /locus_tag="OO326_000800"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5965293.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37032..37775
                     /gene="zapD"
                     /locus_tag="OO326_000801"
     CDS             37032..37775
                     /gene="zapD"
                     /locus_tag="OO326_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5965294.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37785..37982
                     /gene="yacG"
                     /locus_tag="OO326_000802"
     CDS             37785..37982
                     /gene="yacG"
                     /locus_tag="OO326_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5965295.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO326_000803"
     CDS             complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO326_000803"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5965296.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO326_000804"
     CDS             complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO326_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5965297.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO326_000805"
     CDS             complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO326_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5965298.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO326_000806"
     CDS             complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO326_000806"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5965299.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO326_000807"
     CDS             complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO326_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5965300.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO326_000808"
     CDS             complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO326_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5965301.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO326_000809"
     CDS             complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO326_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5965302.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO326_000810"
     CDS             complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO326_000810"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5965303.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO326_000811"
     CDS             complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO326_000811"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5965304.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO326_000812"
     CDS             complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO326_000812"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5965305.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO326_000813"
     CDS             complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO326_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5965306.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO326_000814"
     CDS             complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO326_000814"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5965307.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO326_000815"
     CDS             complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO326_000815"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5965308.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO326_000816"
     CDS             complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO326_000816"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5965309.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO326_000817"
     CDS             complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO326_000817"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5965310.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO326_000818"
     CDS             complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO326_000818"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5965311.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO326_000819"
     CDS             complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO326_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5965312.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO326_000820"
     CDS             complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO326_000820"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5965313.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO326_000821"
     CDS             complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO326_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5965314.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16733 c        14848 g        14367 t
ORIGIN      
        1 caaaggttgg tactgaaaac gggcaactcc ttggtaatac tctcacaggt aatgatgcag
       61 caaaaggagt cggtgtactc atagaaggtt tagcaactag taaaaatcct ctaatgacat
      121 tgaaacctaa tgattcaaat tctgtttata aagattacga cccgagaggc aaagacgata
      181 caacaggagg agtttacccg gatcaagata caggtataac ataccctctc catttccagg
      241 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
      301 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
      361 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
      421 ttatcaggat acgttatgaa accgaccacc attgcttcac ttcagaaata taagcaggat
      481 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
      541 gaagggctta acgtcatgct ggtaggcgac tcgctgggca tgaccgttca gggccacgat
      601 tccactctgc cagtaaccgt tgctgatatc gcctaccaca ctgccgccgt acgtcgcggc
      661 gcaccaaact gcctgctatt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
      721 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
      781 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
      841 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt acaggggcgc
      901 ggcaatgaag cgagcgatcg actactcagc gatgcattag ccttagaagc cgcgggggca
      961 cagctgctgg tgctggaatg cgtgccggtt gagctggcaa aacgtattac cgaagcactg
     1021 gcgatcccgg ttattggtat tggtgcaggc aacgtcactg acgggcaaat cctcgtgatg
     1081 cacgacgcct ttggtattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     1141 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     1201 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     1261 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     1321 tgcctactat gggtaatctg cacaatggcc atatgaagct ggtcgacgaa gccaaagccc
     1381 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     1441 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     1501 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     1561 cttacgttga cgttcctggc ctttcgacca tgctggaagg tgccagccgt ccgggacatt
     1621 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagccg gacatcgcct
     1681 gcttcggtga aaaagatttt cagcaactgg cgttgatccg caaaatggtt gccgatatgg
     1741 gcttcgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa
     1801 gttcccgtaa cggttatctg acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag
     1861 ttttaagttc gattgctgac aaattgcagg ctggggaacg ggatctcgat gaaattatta
     1921 ccattgcggg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     1981 atgccgacac attgctggaa gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg
     2041 cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg taatacttaa
     2101 ctggcgctac ggctgatggc gccagttatt aatttacccc acgtcatctg tcataacaca
     2161 ggtaacttaa ataaggttaa ttaccttatc aatctcagca agaggtaaat ttgccatctc
     2221 tgcaacgtct tcccgagaca ttcctttact tagaagacgc agggcgaatt cttgcctttc
     2281 ctcctgtctt ccctgctgaa tccccttctc aatccctttc tcttcaaacc actgcgccag
     2341 cgtcatcata gacttccctc ccgtttccct gtctctcagc acaccataaa acaaatccgc
     2401 ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact gacttccgct
     2461 agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta agtcgcgctg
     2521 gcgaatatgt ttttgcagta gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc
     2581 atccggtgtg atggtgatat ccaccagcgg gaaaggactg ttatagacgc gtcgcgccag
     2641 ctccggcgag taaaacatat caaaccagca cattgagagc ggataaggtg tggcctcgcc
     2701 ctgataaaac agtatcggca ccaccagcgg cagcttatcg tggccagcct ccagatgccg
     2761 gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat ccggcttgct
     2821 ttggtgttca atcacaacat gcagataacc gggattgccc tgcatttgca tggaatagag
     2881 cacgtccgtg ctgtgacctt tcaggcactc ttcaatgaaa ctccccgact ctaaatgaag
     2941 cgtgttgaga tcacaaagtt cgcgtaattc cactggcaaa tgtatctcca gaaagtcgcg
     3001 agccgtctcc gcatgcatta aaaattgttt aaataccgcg tcatgcggtg tggtactcgg
     3061 tgcatccatc ttccctgtct tccgtgtggc cttgcgtgaa ggcagtatcc agtcaaggcg
     3121 tgctatcgtc agccacctct ttattgttta ccgagcagcg ttcataaaaa tatcaattcg
     3181 ttacaggcgt tacatggcac gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc
     3241 agatgagtag acactcatca aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg
     3301 caccgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg cacgatgctg
     3361 cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga aggttcttgc
     3421 gccattgacc aggattttct tgacgcagcc ggtattctcg aaaacgaagc catcgatatc
     3481 tggaatgtca ccaacggcaa gcgtttctcc acttatgcca tcgcggcaga acgcggttcg
     3541 agaattattt ctgttaacgg tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc
     3601 atcgccagct tcgttaccat gccagatgaa gaagctcgca cctggcgacc caatgtcgcc
     3661 tattttgaag gcgacaatga aatgaaacgt accgcgaaag cgattccggt acaggttgct
     3721 tgattgttat ccctgcggct ggttactcac cagccgcgac atcttctcca gcgaatccgt
     3781 tcttaagata taaagtcgtt ttagtaacaa cggattatcc cccggtttta ctttgccttt
     3841 catggttgtc accgccaggt gaaatccggc atcgtttgct gccttcacgg ctttgtcatt
     3901 aaagccgcca aacggatacg aaagatacca gacatgtgga ttaaattgcg ccagagcgcg
     3961 gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta cggctcagta atatgggtcg
     4021 gcgataacca tctacccgat gcaaaaaatg ggtatgtgac tggaaatcaa aaacatcgcg
     4081 aatttcgtta agctcagaaa cgctcataaa ttgcagcgat tttgggttcc acttctgcgg
     4141 gtgacgtttg atgcgtgagg taacaataaa caccgtcgcc ttcatgccat attgtttcaa
     4201 cacaggatac gcatagcggc tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc
     4261 tcgcgcaggg agattgatcg tattcttcac gtaaccttcc agctgagcca tgctcagtgt
     4321 cgcgtatccc ctgtcgcgca gccaggccat ttggttattg aaagcgcgta ccgaggtggt
     4381 cgtcgaagta tggcgaaaac gggtgttttc ttcgtcgcgc agaatatggt gataagttag
     4441 caccggcagg ccattatcgg gttgggcatc cagcgcgctg atataggcca gtcgatcgcc
     4501 aatacggatc tgataccagg tttgatttaa cctgtctttc agtttatgca aaatcgggta
     4561 gcgcaaattg tccgccagta ccccaaatgg cgcactgccc acactcggcg cgttgtagac
     4621 cggcgtatct ttccaggtaa ttaagttctg attactcagc ggcttgttga ggtcacccaa
     4681 accgtcttca actttttgtc gtccctgaac tggctcgaga tgccctttat cgataaaccc
     4741 tttaccaaag ccaaaattaa atgcgtaata acttgcggca gtgggctcca cggcaatgat
     4801 ttgtccggcc cgaatattcc ccacggtcac catcttgtca ctaatttgcg cccagaccgc
     4861 agcattttcg atggtttgca tataacgggc aggtaacgcg gcactgacac ttgcggtaaa
     4921 cagcatcagc aggagaataa cagcttgttt atacatgaaa aaataaccga gggtgaaaca
     4981 acgcttttat tgtagcaaaa gcgccatcag aatcgcgggt taatactcat acaaattatg
     5041 tttgaggacg aaaggcggat ttttttgttg ttggtgccaa agactactca cctccggccc
     5101 acccagttcg acaatacact cacgaaactc tgaactggtc atggtttcac ggcaagccat
     5161 catctgttca attaacggta acgtgacacc agaaatcact tcgcaacggg agtgtttgtg
     5221 gcttaataat gaagccacgc gatacggtgg cgctccggct atatcggtta agaagatgac
     5281 accctcacca gagtccgctt catgcagagc atcgcacatc atgcggctga gcatattaga
     5341 gcttaatccg cgccagaaat tcacggcccg gcactgaaga agtgccccat gttttttctc
     5401 cagcgcatcc agtatctctt gcgcccggtc atcgtgacag gtaattaccc aacctaacat
     5461 cgccttcctc cttagccgaa aatcagccta ccctgaccat tgttgataaa gggtgatagc
     5521 cgtcaaaact cctctccccc gaatccagag gagaggaaat agccttagct acgcaaacca
     5581 cgtccacgtt ggatcagcga ccaacagatc aaataaaacg ccacaataaa gaccaccagt
     5641 acgccaaagg tagtgaccag cggcacatca tttataccga ggaagccgta gcggaaacca
     5701 ctgatcatat aaacgattgg gttcaggtgc gacagccctt gccagaacgg cggcaacaaa
     5761 gtcagtgagt aaaagacccc gcccaaatac gtgagtggcg ttaacacaaa ggttggcacc
     5821 aggctgatgt catcgaacgt tttggcaaac acgccgttca gcaaacccgc aagggagaat
     5881 agcaccgccg tgagcaccag ggttaaggca acgaataccc acgaatgcac ctgaaacggc
     5941 acaaaaaaca gtgaaattgc cgtcacaaga atgccaacaa acagaccacg cgccacgcca
     6001 ccgccgacat atccggcaat aatgacgtga gtcggaaccg gcgctaccag cagctcttca
     6061 atattacgct ggaacttggc accaaaaaat gatgacgcga cgttagcgta ggcgttggtg
     6121 atcaccgaca tcatgatcag ccccggtacg atgaactgca tatagctgaa gccatgcata
     6181 tcgccaatac gcgaacctat caggttaccg aagataataa agtaaagggt catggtgatg
     6241 actggcggca ccagcgtctg cacccagata cgcataaagc gatggatctc tttcgcccag
     6301 atgcttttta gcgccaccca gtaaagatgc atcatgcgcg atctccttgt ttttcattaa
     6361 ccagtgaaac aaacagctct tccagacggt tagctttgtt acgcatactt aatacctgaa
     6421 tgccctgctc acttaactgc gtaaatacgc tgttgatccc ctgctcacgt agcacttcaa
     6481 cttccagcgt cgcggtatcg accagtcgat actgatagcc atcgagcttc ggtaacggac
     6541 ttttcggtgc gagatcgaga ataaaggttt ccgatttcag cttcgccagc agcgccttca
     6601 tcgaggtatt ttccaccagc tcaccatgtt gaataatgcc gatattgcgg cacagcattt
     6661 ctgcttcttc caggtagtgt gtggtaagaa tgatggtggt gcctttgtcg tttaaatcct
     6721 tcaaaaagcc ccacattgag cggcgaagtt caatatccac gcctgcggtc ggttcgtcga
     6781 gaatcagtag tttaggttca tgcattaacg cacgggcaat cattaaacgg cgcttcatcc
     6841 cgccagataa catacgcgca cgttcgttgc gttttcccca taaatcgagc tgcttaagat
     6901 atttttcgct gcggatgtac gcctctttgc gctccacacc gtagtaccct gcctgattca
     6961 ccacaatttg ctgcacggtt tcaaacgggt tgaagttaaa ttcctgcggc accagtccca
     7021 actgacgttt agcgttcacg acatctttct cgagatcgta accaaatacg ctgacccgcc
     7081 cggaggtttt atttaccaga gagctgataa taccgatagt ggtcgatttc ccggccccgt
     7141 tcggcccgag aagcgcataa aaatcacccg cttcgacctg caaatctatt ccacgaagcg
     7201 cctgaacgcc gcctggatag ttttttttaa gctgttgaag ttccagtgca atggtcataa
     7261 atttttactt accttacgtt cttacacttt atctgtggtt taaatcgtcc cggagttgcc
     7321 ctatattagc caaacgtaat tatttggtta caggtcgtta acctccatga aagacataga
     7381 tacactcatc agcaacaatg cactatggtc aaaaatgctg gtggaagagg atcccgggtt
     7441 ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta tggattggat gttccgacag
     7501 tcgcgttcct gcagaacgtt taaccggtct tgagccgggc gaactctttg ttcaccgtaa
     7561 tgttgctaac ctagtcattc acaccgacct gaactgcctt tccgtggttc agtatgcagt
     7621 ggatgtactc gaagttgaac acattattat ctgtggccac tacggttgcg gcggcgtaca
     7681 agccgccgtt gaaaacccgg aactggggct tatcaacaac tggctgctgc atatccgcga
     7741 tatctggttc aaacatagct cattgctcgg cgaaatgccg caagagcgcc gtctggatac
     7801 cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg ggccactcca ccattatgca
     7861 atcagcgtgg aaacgcgggc agaaagttac cattcacggc tgggcctacg gcattcacga
     7921 cggcttgctg cgtgatctgg atgttaccgc caccaaccgc gaaacccttg agcaacgtta
     7981 ccgtcacggg atttccaacc tcaagctgaa acacgccaac cacaaataaa aatgccatgc
     8041 cggatgcaac acatccggca acttcacact tactcgtcca gctgaatcac tttgccaata
     8101 tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc
     8161 gggatcgaga aaccgataaa ttcaaccggg acgttcactt cacgacggga cggtttatcc
     8221 agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc ttaagatctc acgcactttc
     8281 gacagtgtat tccccgagtc gatgatatct tcaacaatca gcacgtcctt gccacggata
     8341 tcttcatcca gatctttgag gattttcaca tcacgggtgg tggacatgcc gctaccgtag
     8401 ctggaggcgg tcataaagtc gacttcatga gatacctgaa cttcacggca caggtccgcc
     8461 ataaacataa atgagccacg cagcagaccc accagcacca tatcgctgcc gctgtctttg
     8521 taacgctcag taatctgacg acccagttcg gcgatacgcg ctttaatctc cgcttcgggg
     8581 atcattactt ctacagtatg tttcatatct ctaaccatat gatttaaaaa caaatcactt
     8641 cgcacgggca ggttttggca ccgcaacaaa gcgcaagacg cgcagtatac cagtaaaact
     8701 attcattcgc ggaatctgtt aataaagctc gaattgtgat gacggtcaca catgttaaaa
     8761 cctataatta attgctacta aaatattaat gaattgaaat ggtgtctctt tatggcaatt
     8821 aacaatacag cctcgcgacg attactcgtc acgctaacag ccctttttgc agcgctttgc
     8881 gggctgtatc tactcattgg cggaggctgg ctggtcgcga ttggcggctc ctggtactac
     8941 cctatcgctg gccttgtgat gctcggcgtc gcctggatgc tgtggcgcag taaacgcgcc
     9001 gcgctttggc tatacgcagc cctgctgctc ggcaccatga tttggggcgt ctgggaagtt
     9061 ggtttcgact tctgggcgct gactccgcgc agcgatattc tggtcttctt cggcatctgg
     9121 ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca
     9181 ctggtggtcg cactgctgat tagcggtggt atcctgacct gggccggatt taacgatccg
     9241 caggagatca acggcacctt aagcgccgat gccacacctg ccgaagctat ctcccccgtt
     9301 gccgatcagg actggcctgc gtatggccgc aatcaggaag gtcaacgctt ttcaccactg
     9361 aagcaaattc acgccgataa cgtccacaag ctgaaagaag cctgggtgtt ccgtactggc
     9421 gatgtgaagc agccgaacga tccgggtgaa atcaccaatg aagtgacgcc gattaaagtg
     9481 ggcgataccc tttacctgtg taccgctcac cagcgcctgt ttgcgcttga tgccgccagc
     9541 ggcaaagaga aatggcatta cgatcctgag ctgaaaacca acgagtcttt ccagcacgta
     9601 acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg
     9661 gcggattgcc cgcgtcgtat cattcttcca gtgaatgatg gtcgcctgat tgcgattaac
     9721 gctgaaaacg gcaaactgtg cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc
     9781 aatatgccag acaccaaacc gggtctgtat gagccgactt ctccgccaat tatcaccgat
     9841 aaaaccatcg tgatggccgg ttcagtcacc gataacttct caacccgcga aacgtctggc
     9901 gtgatccgtg gttttgatgt caacaccggg gagctgctgt gggcttttga tccgggcgcg
     9961 aaagatccga acgcaatccc gtcagacgaa cacaccttta cctttaactc accaaactcg
    10021 tgggcaccag cggcctatga cgcgaagctg gatctggtct atctgccgat gggcgtgacc
    10081 acgccggata tctggggcgg taaccgcaca ccggaacagg aacgttatgc cagctcgatt
    10141 ctggcgctga atgccactac cgggaaactg gcgtggagct accagaccgt tcaccacgac
    10201 ctgtgggaca tggatcttcc ggcacagccg acgctggcgg acatcaccgt taatggtcag
    10261 aaagtgccga ttatttacgc tccggcgaaa accggtaaca tttttgtgct cgatcgtcgt
    10321 aatggcgaac tggtggttcc ggccccggaa aaaccggttc cccaaggtgc tgccaaaggc
    10381 gattacgtta cccctactca accattctcg gaactgagct tccgtccgac gaaagatttg
    10441 agcggtgcgg atatgtgggg agccaccatg tttgaccaac tggtgtgccg cgtgatgttc
    10501 caccagatgc gctatgaagg cattttcacg ccgccatctg aacagggtac gctggtcttc
    10561 ccgggtaacc tggggatgtt cgaatggggc gggatttccg ttgatccaaa tcgtgaagtg
    10621 gcaattgcca acccaatggc actgccgttt gtttcgaaac tgctcccgcg tggtcctggc
    10681 aacccgatgg agcagccgaa agatgccaaa ggcacgggta cggaatccgg cattcagcca
    10741 cagtacggtg taccgtatgg tgtcacgctc aacccgttcc tctcaccatt tggtctgcca
    10801 tgtaaacagc cagcatgggg ttatatctcg gcgctggatc tgaaaactaa tgaagtggtg
    10861 tggaagaaac gtattggtac gccgcaggac agtatgccgt tcccgatgcc ggttccggtg
    10921 ccgttcaata tgggtatgcc gatgctgggc gggccaatct ccacggcggg taacgtgctg
    10981 tttatcgccg ctacggcaga taactacctg cgcgcttaca acatgagcaa cggtgaaaaa
    11041 ctgtggcagg gtcgtttacc ggcgggtggt caggcaacac cgatgaccta tgaagtgaat
    11101 ggcaagcagt acgtggtgat ctccgcaggc ggtcacggtt catttggtac gaagatgggc
    11161 gactatattg tggcttatgc cctgccggat gatgtgaaat aaccataatg cccggagaga
    11221 tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc atccccgtat
    11281 cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt gcgttgtgat
    11341 taaacttcac cagcacttca ctgacattgc cttctacctt aacggtatct ttccagcccg
    11401 cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc gtgccgtgaa
    11461 tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt tcgtactgcc
    11521 ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg attttattgg
    11581 catggtggaa atcgaacttc ccgccgtggt tcatatgatt catattgccg tgccccatat
    11641 ggcccatcat ctggctgtga tccattccgg ccatcgcctg atcgccatat ttctccatta
    11701 gcatctgcat ccccatcata tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg
    11761 tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtcaggc aaagcaccgg
    11821 aggcactaat agcaatcggc tgaatccgca ttaccggatg cggcttgtca aacggtgcaa
    11881 tcaccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa
    11941 cctccaccag cacttcaaaa cgctcgccca tcagcaccgg cagctcgctc accttcaccg
    12001 gttcaggtag cagaccaccg tcgctggcaa tcacatacag cgggcgattg tcgctggtgg
    12061 cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc caaccacgcg
    12121 gggcagcgtg ttgtgggtag attgcaccgt tggtcagcag cgtatcgcca aaccagccca
    12181 cggcggcggt catcacatcc agttgataat caatctgccc gtcggcgcta aatttcttat
    12241 cctgaacgat caccggaaca tcatcgatac cccactgttt tggcagcatt aatttcagga
    12301 tctcgtcatc ttcaatcacc accagaccag ccagccccat cgccacctgt cgcccggtct
    12361 tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca
    12421 ccgagcgctt gccacctggc ggaataattc cctgtgggcc gccgtcgacg tcacccggta
    12481 cttccagccc gtgccagtgc aacgtcgtct cttccgccag ttggttgtag atatcaaccg
    12541 ttaccgcttt gccgcgctgt aatttcaccg ccggccccag cagattgccg ttatagcccc
    12601 aggtagttgc agttttctcg ccaaaggtgg actgacctgc gccaatagtt aactgaatgc
    12661 gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa
    12721 agactgcacg gctccacagt ggcaaggctg aagccacacc cagtgcgacg gaatatttca
    12781 agaaatcacg acgttgcata gttatttcct tattcttaag caggcgaaac aaaatcaaac
    12841 gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt taatcgacaa
    12901 taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc ccctggtaga
    12961 agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatcgccg tttgcgcaaa
    13021 cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg cagtttttgt
    13081 ctttctgaag aacgattgtg gttaccagaa cttatctaac gggcaaattc gtcgcgcact
    13141 ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct tcgacatgaa
    13201 agctctcggt gaagacagct accgcgatct cagcggcatt ggcattcccg tcgctaaaaa
    13261 atgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat aatccctgat
    13321 acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt atgttgcgcc
    13381 ctttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa aacagtggca
    13441 tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc tgtatcatga
    13501 aaagaccgat caccaggatc tgatcatttt tgagaacgct gcatttggtc gcgtaatggc
    13561 gctggatggc gtagtacaaa ccaccgagcg cgacgagttt atctatcatg agatgatgac
    13621 ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg gcggcggcga
    13681 cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca cgatggtgga
    13741 aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctg cccaaccata acgccggtag
    13801 ctacgacgat ccgcgctttc aactagtgat cgacgatggc gtcaatttcg ttaatcaaac
    13861 cagccagacc tttgatgtca ttatctccga ctgcaccgat cctatcggtc ccggcgaaag
    13921 cctgttcacc tcagcatttt atgaaggctg caaacgttgc ctgaatcctg gcggtatctt
    13981 cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa gccatcgaca gccatcgcaa
    14041 actcagccat tacttcagcg acgttggctt ttatcaggcg gcgatcccga cctattacgg
    14101 cggtatcatg acttttgcat gggcgacaga taacgacgcc ttacgccatc tctcaaccga
    14161 aattattcag gcgcgttttc tcgcttctgg cctgaaatgc cgttattaca atccggcaat
    14221 ccatacggca gcttttgcct tacctcagta tctgcaagac gcactggctt cacagccgtc
    14281 ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata atctgaccaa
    14341 aagtctgagt ttttgtattt acgatatctg ctacgccaaa actgccgaag agcgcgacgg
    14401 ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga
    14461 aacctgttcc attatcgggg ccaatattct taacatcgcc cgccaggatt acgaaccaca
    14521 gggtgccagc gtcactattc tggtgagtga agaaccggtt gacccgaaac tcatcgacaa
    14581 aacagaacac cccggcccac tgccagaaac ggtggttgct catctcgata aaagtcatat
    14641 ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga
    14701 tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt acctgatcca
    14761 ccagcttgag tccgatatcg taaccattga ttatcgcgtg cgcggtttta ctcgcgacat
    14821 taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact ttatgtctga
    14881 cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc tatcaggaaa atatcttcca
    14941 taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca ccaaaccgga
    15001 agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga
    15061 gatttattac gggcgcaata tgccagctgt ttaacggctc tggcggagct cccaggctcc
    15121 gccagattta tttacttctg ctgcacgaaa ttgcggtaag ccgccacgac ctgcagaaaa
    15181 tcctcaacgc cgcatagcga caggctttct tcgtcgtagt agttcatccc ctcttccatt
    15241 tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcagc gtccatccac
    15301 agggtgtatt catgccctgc ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct
    15361 gcttgttcca cttcatcaag caaggccagg ttttctttca cctcttcatt aaaccagtgc
    15421 ccgaccactt catgccccat ggacatacgc acctttacca ctccggtaat atcgcgcaga
    15481 aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc gcatcgcgcc
    15541 attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat agtttagaaa
    15601 taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    15661 cttacttatt acaccgcagt ctggaaaatc accccatcgg ctttttcggt gtactgagaa
    15721 agctggttga agttcagata acgataagta tcaacggcag ttttatctac ttgcgcaacg
    15781 taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agccgccagt
    15841 tctgcagaag ccaggaagac attcgcgcca gtacccaaac ggttcgggaa gttacgggta
    15901 gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct ggttacccat acacagggaa
    15961 cagcccggga tctcgatacg agccccgctc ttaccgaaga cgctgtagta gccttcttcc
    16021 gtcagttgcg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga
    16081 cctttgtgcg catccagcag tttaccagcg gcacggaagt gaccgatgtt agtcatgcaa
    16141 gaaccgataa atacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtca
    16201 tccggatcgt tcggagcaca caggattggc tctttaatct ccgccagatc gatgtcgatc
    16261 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    16321 tccatgccct gaatacggcg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc
    16381 cacttcagca ggacgatgtt ggagctcagg tattcgatga tcggttcttt gttcagcttg
    16441 atagtacaac cagcggcaga acgctcggcg gacgcatcgg ttagctcaaa ggcctgctca
    16501 actttcagat ccggcagacc ttcaatttcc agaatgcgac cagagaagat gtttttcttg
    16561 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    16621 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    16681 ggcatatcca gcggcattac gccagttgcg gcagcaaacg ccaccagacc agaacccgcc
    16741 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    16801 ggcagcagca tacggttcag ccaggagtga atgacgccgt cacccggacg cagcgacaca
    16861 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    16921 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    16981 gccaggtctt tcagttcatc acgggtcatc gggccggtgg tgtcctgaga gcctacagaa
    17041 gtcattttcg gttcgcagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    17101 accattttct gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    17161 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgcccg
    17221 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    17281 tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca cttcaccttt gtacgggtaa
    17341 acgtcaatca cgtcgcccat gttcaggtta gagacgttga cttcgattgg cagtgcaccc
    17401 gcgtcttcca tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg
    17461 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg
    17521 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    17581 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    17641 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc cgggcgtgac
    17701 cacgcatccg gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttaaagacg
    17761 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atccgcccag
    17821 gactgcatta cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag
    17881 aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcaatagg tgccagtttg
    17941 gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat ggtgcccagc
    18001 agttcgatag ctttttccgg agtcagcaga ggggatttag cttcgccttt agcgatagca
    18061 gccaggaagc ctgcttttac ataggcggct tcatcgacgc ctgggggaac acggttggtt
    18121 aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc
    18181 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    18241 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    18301 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    18361 caacgggcag aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata
    18421 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    18481 taattcgcat ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    18541 taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg
    18601 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    18661 taatttgcag tgccgggctt tgtgctgcct ctgccgggct atatataaaa agccgcacag
    18721 tggaagcgcc tgtagaaccg caatcgacac aactggctgt gtctgacgct gccgctgtta
    18781 cgcttcctgc aacggtttcc gcacctcccg taatacccgc agtcgtcaaa tccgcattca
    18841 gcactgcaca aatagatcaa tgggtcgcac ccgtcgcgct gtatcccgac gccctacttt
    18901 cgcaggtgct gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc
    18961 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    19021 acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc
    19081 cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaggac gtgatggact
    19141 cggtacagcg attgcggcaa ctggcgcaac aaaccggctc gctgaagtca tcaaccgaac
    19201 agaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggcacccg
    19261 tcataccatc caataccgtt ttaactgcca accccgtcat tacagagccg gcaacaaccg
    19321 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
    19381 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    19441 gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta tagcatgggc gttgctacca
    19501 cgtacgcact attcagcagc atcgactgag atgacgacga tcatgaccat catcatcatg
    19561 acgatgatga ttatcatcac cacgatggcg gtcatcgtga cagtaatggc tggcaacaca
    19621 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    19681 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    19741 atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg agtgccacgc
    19801 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    19861 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    19921 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    19981 cactgaccca gcagcaaaag gacgccgctc gtcagcgtta tcagtcggcc tctcctgagc
    20041 agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gatccagcag cgaagggagg
    20101 cggcgcgcca gcgtattcag tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa
    20161 tgcagactaa cccgcagaac cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg
    20221 caactcccga acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    20281 agcaacgtga taacgcccgc cagcgtatac aatcggcatc acctgaacaa cgtcaggttt
    20341 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    20401 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgaacgt ggagcaagaa
    20461 gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    20521 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt
    20581 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    20641 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    20701 acccagcagc tcgccgccgt tggtaccaac aatcgcacca ccgatcacac ggtgagattc
    20761 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtca gaagcgatag cacgaccaga
    20821 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    20881 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    20941 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    21001 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    21061 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt
    21121 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    21181 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    21241 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    21301 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    21361 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    21421 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    21481 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    21541 aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt
    21601 gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    21661 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    21721 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    21781 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    21841 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    21901 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    21961 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    22021 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    22081 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    22141 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    22201 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    22261 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    22321 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    22381 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    22441 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    22501 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    22561 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    22621 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    22681 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    22741 cgacagcgaa ctattgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    22801 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    22861 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat
    22921 catcacccag ttacggctca ggttagcacc agaaattttc tggatacggc ccagttccac
    22981 ttcttcgatt tcgccaaact tgctgaagtc caccttcggc cacggcagca tgccagggat
    23041 accaccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    23101 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    23161 aacgccaaac tcgcgtgcca gacggcggat cagcggggtc gcgtgaacgt aagcgtcgtt
    23221 ttcagcaaat tcagatttgc cttccgcttt cgcagccgga gcttcagctt ttgctgccgg
    23281 agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc
    23341 gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt
    23401 cacgacgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtaat
    23461 cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac
    23521 ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcaggggt
    23581 cgctgccgga gtcgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac
    23641 ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc
    23701 tttcacggta cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt
    23761 gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc
    23821 aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc
    23881 tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc
    23941 gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac
    24001 tttgatctct ttaacgatac ccgcctgcgg agatggaact tccatagagg ctttgtcgcc
    24061 ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc
    24121 ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct
    24181 tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt
    24241 gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca
    24301 accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa
    24361 cgaccgaagc catcagtacc cagtacgcga tagtcgtcag ccggtacgta agtacggacc
    24421 tgctcagcga acagtttcat atagtcggta gatgccactg ccggcgcgtc gttcatcacc
    24481 tgagcgatat acggaacgcg cggagtttcc agcggatgca gcatgttcca gcgttcacaa
    24541 tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg
    24601 ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat ggaaccggag
    24661 cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta
    24721 cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta
    24781 gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc
    24841 ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc
    24901 agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta
    24961 cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtctccagc cgcccagcac
    25021 agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaatgg gatcatcggc
    25081 agattgttcg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg
    25141 ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg
    25201 cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga
    25261 ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc
    25321 gacttgttct tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagagctct
    25381 ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg
    25441 gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga
    25501 taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca
    25561 tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc
    25621 ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg
    25681 gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg
    25741 tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag
    25801 tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca
    25861 ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc
    25921 agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg
    25981 atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta
    26041 ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc
    26101 agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg
    26161 tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga
    26221 tatttcagga atttagcctg gtaaatagca ccaatcggac ccagacccat agatacggtc
    26281 gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg
    26341 tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca
    26401 cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc
    26461 tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg
    26521 aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg
    26581 atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac
    26641 tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct
    26701 acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc
    26761 tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc
    26821 ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt
    26881 tgagttttct ggaacctgtc ccattgaact ctcgccagaa agctcaataa gacaggttct
    26941 acgtttagtt accgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta
    27001 ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct
    27061 ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc
    27121 ttcgcgcgct tcttccggct taccgaccat aatcgcttca aatatgcggg tgcggtgact
    27181 actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt
    27241 ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc
    27301 ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga
    27361 ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt
    27421 actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa
    27481 gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg
    27541 ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc
    27601 ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag
    27661 ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc
    27721 cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag
    27781 ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc
    27841 taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt
    27901 gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtag
    27961 actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc
    28021 gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcactctgc
    28081 atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc
    28141 aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag
    28201 tctttttgta acaattcaaa cctccttgat gtaaacaaat caatacaaca aacggaattg
    28261 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
    28321 atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag
    28381 cttatcgcgc tgggtggcgc gatagggaca gggctattcc taggtagcgc ctccgtaata
    28441 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
    28501 atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
    28561 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
    28621 ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg
    28681 tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc
    28741 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
    28801 attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc
    28861 ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc
    28921 ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg
    28981 atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcaac taaccaggtt
    29041 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg
    29101 tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat
    29161 acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac
    29221 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg gaacgcgcca
    29281 aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg
    29341 ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg
    29401 ttaatggcac tggtggtatc cgcactggtg atcaactggg cgatgatcag tctggcgcat
    29461 atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt
    29521 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
    29581 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
    29641 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
    29701 gccctcaccc gtacagggtg agggcaataa tctctacacc aacgccccgt aaatcgtcag
    29761 tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt
    29821 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt
    29881 taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc
    29941 tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
    30001 cagtacatga agcactgcat caatgccgga ctgtaaacga tgatgcggcg tctggtatcg
    30061 cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa cgggtcccca
    30121 ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag
    30181 caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg
    30241 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
    30301 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
    30361 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
    30421 taaaaaagtc acacccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg
    30481 tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt
    30541 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
    30601 ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc
    30661 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt
    30721 gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg
    30781 ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag
    30841 actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg
    30901 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc
    30961 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
    31021 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccag gcggcaatcg
    31081 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
    31141 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
    31201 cgttagctat ctggagtttt aacatgccgc ctcgccgcta caaccctgac acccgacgtg
    31261 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
    31321 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
    31381 attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt ggcaaacgct
    31441 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
    31501 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
    31561 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
    31621 gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc
    31681 gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
    31741 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgtct
    31801 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat gcgccagtag
    31861 aagtcgaagt agagaatctg gaagaacttg atgaggccct gaaagccgga gccgatatca
    31921 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
    31981 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
    32041 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
    32101 caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
    32161 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata
    32221 aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt
    32281 ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt
    32341 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagctc cattggtatt
    32401 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
    32461 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
    32521 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
    32581 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
    32641 acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa
    32701 agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
    32761 ggagcggcaa tgaatattcc acagctcacg gccctgtgcc tgcgttatca gggagtcttg
    32821 ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc acatgagttg
    32881 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
    32941 caaatggaag gtcacgccag tcgcacacaa cagacattac ccgtagctgt tcaggagaag
    33001 catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgcgcg
    33061 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
    33121 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac agccagatta
    33181 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
    33241 gtcgaactgg caggaaacac cgtctcattt cgtattgcga ccttaccatg tcggggtggt
    33301 gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt taacacgctg
    33361 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
    33421 gtgctggtaa ctggccctac aggcagtggc aaaacggtca cctggtgttg tccacgctac
    33481 acaccaattc caccactgaa acgctggtac gtttacagca aatgggggtc gcccgctgga
    33541 tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgtaaa ctttgcccac
    33601 attgtcgccg acagcaaggg gagcccatcc atattccaga cactgtatgg ccgtcgccgc
    33661 tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtaccg
    33721 ccttgtttga agtcctgccc attacaccag ccatacgcca gcttatttcc gctaatactg
    33781 aagttgaatc gctggaaacg cacgcccgac aggcgggtat gcgtacgctt tttgaaaacg
    33841 gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga
    33901 tgccgcatgg caagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg
    33961 caagatggga tgctatgggc agagagccgt actttactgc ttatggcact acagcaacag
    34021 atggttaccc cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat
    34081 aaaagcgcgg aagtcattca tcaactggcg acgctactta aagccgggtt aacgctttct
    34141 gaagggctgg ctctgctggc ggaacagcat cccagtaagc aatggcaagc gttgctgcaa
    34201 tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt accctggtca
    34261 gaggcatttc cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg
    34321 gatgaatgct gctttgaact ggcgcgtcag caaaaatccc agcgtcagtt gaccgacaaa
    34381 gtgaaatcag cgttgcgtta tcccatcatc attttagcga tggcaatcat ggtggttgtg
    34441 gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt caacacccca
    34501 ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg
    34561 ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat gcgccgcccg
    34621 acctggctta tcgcgcggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg
    34681 aggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag tgcaggcatt
    34741 acttttttac agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa
    34801 cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct ggcgctaaaa
    34861 aatgccggtg agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc
    34921 tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg
    34981 gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc
    35041 ggtacgctgg tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtggg
    35101 atgggataac gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga
    35161 tgcggttttc ttgttccagc acacgaataa acgtggtgcg cttggtcagc tctttcaggc
    35221 gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat
    35281 tttcaacctg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cggtattccg
    35341 caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa
    35401 atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc
    35461 cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt cgccacatcg cccggcgtgg
    35521 tacagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg gcacattcga
    35581 ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac
    35641 ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc tcacacattt
    35701 caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg
    35761 caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa acgaagttta
    35821 atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg
    35881 tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact
    35941 cttcgacaga aaagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg
    36001 ccatagaaaa tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc
    36061 tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa
    36121 gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa
    36181 tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac
    36241 gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac
    36301 aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc actttcgcgc
    36361 gctcattttt aacgttttga taatcatgat gaattcccgg gaatataaat tatggggtat
    36421 atagttgcct taacgggagg cattggcagt ggtaagagta ccgttgccaa tgcgtttgct
    36481 gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt
    36541 gcacctgcgc tacatgccat tgctgatcac tttggcgaaa acatgattgc tgctgatgga
    36601 acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg
    36661 cttaacgacc tgctgcatcc gctgattcaa caagagacgc aacaccagat ccagcaagca
    36721 acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa
    36781 gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag
    36841 cgcgatgatg taactcgcga acatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa
    36901 gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca
    36961 tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag
    37021 gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta
    37081 catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc
    37141 acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt ttcgagcgcg
    37201 gcgaagtccg cactgagctg ttgaaagaac ttaaccggca gcaacgtaaa ctccagacct
    37261 ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag
    37321 cggcggtgag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt gaagatcgtt
    37381 tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc tttgatttac
    37441 ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct
    37501 ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc
    37561 agtcggcccc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg
    37621 atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg
    37681 gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac ggacaggtac
    37741 cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta
    37801 cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc
    37861 cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac
    37921 gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt
    37981 gacatttgtc tgatgcctca cgtaggcctg ataagacgcg gacagcgtcg catcaggcat
    38041 cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg cttaagcttc
    38101 gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    38161 cactcaccgg gttgcccttc tttaccccac ggcccccctt cccaactttc gaccagccag
    38221 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    38281 tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg ttccggcgtt
    38341 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    38401 gcgcgacgtg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    38461 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    38521 ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca gaaccgcacg
    38581 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    38641 ctgcagagtc gtcatcctga tggctaagct gctgcatttg tgctaaacgc tcggcttcca
    38701 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    38761 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    38821 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    38881 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    38941 cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta cgctgataca
    39001 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tctggttctt
    39061 tatccagcca ctcggtaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    39121 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    39181 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    39241 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    39301 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    39361 ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt ttacgcatca
    39421 tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    39481 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    39541 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc agtaccgcat
    39601 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    39661 gcgcggcaac ttctgcctgc cagctacctc cgagcacaat atctgtacca cgacccgcca
    39721 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    39781 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    39841 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    39901 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    39961 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    40021 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    40081 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    40141 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgtcggc
    40201 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    40261 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    40321 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    40381 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    40441 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tgacggatca
    40501 ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct gccgggccgg
    40561 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg tccaccagcg
    40621 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    40681 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    40741 ttgccggtgc tggcatgccc ggcaggttga taccaacagt caggccaagg aattcaaaca
    40801 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacgtgca
    40861 cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg gttttacctt
    40921 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    40981 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg gcgaaagctt
    41041 ccgggatcag attttccagc acttcgcctt tttccagacg cgcacgaaac tctgcggttt
    41101 tccctttcag ttcttcatcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    41161 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    41221 acaatttgat tagcataata aaatctcaaa cgccccgcgt tacggagtta ataaaatgaa
    41281 gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    41341 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    41401 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    41461 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt ttgcggtgcc
    41521 attgcgaaag aaagatgacg gattaccgta cgaatagcat gttgatgcca gtaatcaacg
    41581 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    41641 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    41701 gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa gagatgcggc
    41761 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    41821 tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    41881 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    41941 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    42001 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acagctgaag
    42061 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    42121 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    42181 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca
    42241 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    42301 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    42361 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    42421 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    42481 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    42541 cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc aactcgtcga
    42601 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    42661 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    42721 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    42781 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    42841 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    42901 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    42961 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    43021 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgtagg
    43081 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcact
    43141 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    43201 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    43261 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    43321 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    43381 gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag cacgccgcgc
    43441 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    43501 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    43561 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    43621 gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc atccagcagg
    43681 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    43741 acatgcttgg acagttcagt gatcccctgc tcggcgaatg ccatacgctt cttgccttca
    43801 aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    43861 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    43921 atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc cgcattgcgg
    43981 ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc gatttgaatc
    44041 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    44101 acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    44161 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    44221 cgatttgtgc ctgtcgcctg agtccgtaat catcgtcggc ctcataaaaa ttaaaactct
    44281 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    44341 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    44401 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    44461 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    44521 gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    44581 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    44641 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    44701 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    44761 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    44821 acattgccag cataaggaat tacctttgtg tggcgcaatg ccccaccggt ataaacggcg
    44881 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    44941 gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac tttcagccca
    45001 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    45061 acttttgcct gcatccgcac acccgaaagt cctaccggat tcttgatccc ttcctgatag
    45121 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    45181 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    45241 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    45301 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg tacgcacttg
    45361 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg gcagctgccc
    45421 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    45481 gcggtaccaa tttccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    45541 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    45601 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    45661 cctgctgctg taaaaccgga taaagttcta caaagcgaac caaacgtttc atcgtatcgc
    45721 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    45781 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    45841 agccctgcaa cacttcatta gcgctgcctt ccgggccata cagcattgga agcacctgct
    45901 tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc atgtgttgat
    45961 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    46021 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    46081 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    46141 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    46201 gttgcgcatc ttccatccag ctcaacacga cccagccgct caccaacact gtcgttaaaa
    46261 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    46321 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    46381 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    46441 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcactg
    46501 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    46561 actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca gaaatactgt
    46621 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    46681 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    46741 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    46801 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    46861 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    46921 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    46981 ttgctgcgta gtttatctat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    47041 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    47101 gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    47161 tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    47221 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    47281 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    47341 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    47401 cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt tcccacgtcc
    47461 acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt cgccagccgg
    47521 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    47581 caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    47641 ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt ggccgtagtc
    47701 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    47761 gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa tagcctcgtc
    47821 gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    47881 gcctggcgca ttcagggtga cgcacatcgg ctctttgtcc tgacgcagca gcgtaaagtg
    47941 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    48001 gtaagttgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg gatcatcaac
    48061 acacatcacc gcacgaccgt aaaaaggcag gttgtgcaga aaattaataa aggtctgttt
    48121 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    48181 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    48241 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    48301 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tagttgtcgt
    48361 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    48421 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttgtc
    48481 ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    48541 gttgaaataa atcgtcgcac ccagattcag taactgctgc gtgaccggat ttggcgctaa
    48601 atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac cgcccatacc
    48661 ggcaccacca atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    48721 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    48781 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    48841 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    48901 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctcg
    48961 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    49021 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcacagtt
    49081 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    49141 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    49201 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    49261 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccaacaca
    49321 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc cagcacatcg
    49381 gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa cgcctgcatc
    49441 actttggtag caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    49501 tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc tgatacatag
    49561 ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    49621 caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaaa
    49681 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    49741 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagata gtgcgcaacc
    49801 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    49861 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    49921 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    49981 tgattagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    50041 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    50101 ggtcaatttc taatgctttg cggccaatcg acatcgcacg aaaagcgacg aagaatacca
    50161 ttaaaagtgc tagtaccaca ccgacataac ctaattcttc gccgataatg gcgaaaataa
    50221 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgaattacct aaaccttgcc
    50281 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    50341 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    50401 ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    50461 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    50521 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    50581 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    50641 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    50701 aacgcgatgc ccctttaaca gcgctaccca ctaccagcac gatcatcagc aggatgatag
    50761 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    50821 tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg aagaagaacg
    50881 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    50941 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    51001 tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc cagaccagga
    51061 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    51121 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    51181 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg gagccagcaa
    51241 gcgcatagcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    51301 ctgcgcgccg tcacgaccga aacaatacag acgtacgtta tcgccattca ggtaacgcac
    51361 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    51421 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    51481 aatccagcgc acaccgttat gctccagcac cacttcaaag cgatgcggca gaccggtgaa
    51541 ggtggttaac gctttcaggc tgctggcgcg cggtaaccct gcggcatctg ccagcgccag
    51601 cgccaccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt tcacattcag
    51661 cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    51721 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    51781 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    51841 acgatactgt tgtaaaccaa acgggtagcg atccatatga tcttcagtca cgttcagaat
    51901 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    51961 cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    52021 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    52081 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac aaaacagctc
    52141 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    52201 tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat cattcagact
    52261 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt
    52321 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    52381 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    52441 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    52501 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    52561 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    52621 acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac ggcgataatg
    52681 cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg cgccggatag
    52741 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    52801 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    52861 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    52921 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    52981 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    53041 gttgcgggcg tgtctttgcc ggcaaggtac aaagcgaaag caacacccag cgcaatgacc
    53101 gacatccaga aatatttcca acgagcgatc aaccctttgg tgtctttacg caccactttg
    53161 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    53221 acgtacggat tggacgggta agcccacagc agcacggaga tcacaatcgc tgtcaggatc
    53281 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    53341 cgcactacct gaccaaagga aagtttttgc aaatgagcaa tcatgcgcgg gcccatccac
    53401 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    53461 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    53521 tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc ttaactaaaa
    53581 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    53641 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccatgc
    53701 ttaacacgcg gtcaatacca gcagcttttg ccgcctcacc cacctgtaca tggcaggctt
    53761 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    53821 cagccagtac ctgaacagct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    53881 gcagcaactg gttttctgcc agttggatgg ggaacagacg gccaggaaca gctttcagat
    53941 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    54001 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac
    54061 cggttggggt ttgtagggta aattccatac cgtgcgaggt cacatggata ttggtggcgg
    54121 tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag ccaattacgc
    54181 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    54241 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    54301 gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc
    54361 aggcaatctc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    54421 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    54481 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    54541 aggaccccgt cagagcaacc acgagcgccg gaacttgctg gcgaacccat gcagccagtt
    54601 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg tccagcggac
    54661 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcgtgggcat
    54721 caaaacgttc acctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    54781 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atatcggtaa
    54841 gttggctaag ggttacgcta atcatgcaat cccccccagc agacgcgcca ccgtgacgcg
    54901 atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatggc ctttgcccgc
    54961 gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    55021 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    55081 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    55141 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    55201 gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc
    55261 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    55321 catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag ccagtggata
    55381 gcccagcgcc agcagtgttg ccagcgcgag caacaggttg ctgacgttaa aagcgcccat
    55441 cagatggctt tcaatttcgc catcgcccca gcttgaacta aagcgaatgg tcgcgccgct
    55501 gtcgtgatag ttcacttcgg tcgctttcaa ccagcgtccg tgacagttcg gattgatatg
    55561 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    55621 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    55681 agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    55741 gaccgacgcc ggaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    55801 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    55861 aactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    55921 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gaagctgggt
    55981 cgtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt cagagggttc
    56041 atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga gatagatgac
    56101 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    56161 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    56221 acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc
    56281 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    56341 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    56401 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    56461 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    56521 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    56581 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    56641 ctttaatcgc cgccttcacg ccgccgccgc ctggtagcgc cacgctttcc atcatatgca
    56701 ccacggtgcg aacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    56761 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    56821 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    56881 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    56941 cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc atcgttaacg
    57001 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    57061 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    57121 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    57181 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct ttcggcgtgc
    57241 cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact tcacctgtgt
    57301 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcgttgttca
    57361 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg
    57421 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    57481 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    57541 cttgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg gacggatagt
    57601 aacggcgaga ctcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    57661 ccatgtcaga gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    57721 tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    57781 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    57841 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    57901 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    57961 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    58021 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    58081 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    58141 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    58201 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    58261 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    58321 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    58381 atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    58441 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    58501 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    58561 taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct cttcagtcat
    58621 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    58681 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    58741 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    58801 cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    58861 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    58921 ggcgcgggca atgcgtttgg caaaatgctc ttcaccatag gttttcaata cccaggcgat
    58981 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    59041 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    59101 cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccaataa gatcgcgctc
    59161 ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc gcggatcatc
    59221 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    59281 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    59341 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    59401 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    59461 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    59521 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    59581 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    59641 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc aacagacgcg
    59701 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    59761 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    59821 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    59881 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    59941 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    60001 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat
    60061 gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac tcactatttt
    60121 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    60181 cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    60241 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    60301 cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat taacgggcgg
    60361 gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa ggatattgat
//
LOCUS       DAJTRW010000012        59772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRW010000012 DAJTRW010000000
VERSION     DAJTRW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            547..1836
                     /gene="sdaC"
                     /locus_tag="OO326_000822"
     CDS             547..1836
                     /gene="sdaC"
                     /locus_tag="OO326_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5965315.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1894..3261
                     /gene="sdaB"
                     /locus_tag="OO326_000823"
     CDS             1894..3261
                     /gene="sdaB"
                     /locus_tag="OO326_000823"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5965316.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3372..4127
                     /gene="xni"
                     /locus_tag="OO326_000824"
     CDS             3372..4127
                     /gene="xni"
                     /locus_tag="OO326_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5965317.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4180..5328)
                     /gene="fucO"
                     /locus_tag="OO326_000825"
     CDS             complement(4180..5328)
                     /gene="fucO"
                     /locus_tag="OO326_000825"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5965318.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5356..6003)
                     /gene="fucA"
                     /locus_tag="OO326_000826"
     CDS             complement(5356..6003)
                     /gene="fucA"
                     /locus_tag="OO326_000826"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5965319.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6550..7866
                     /gene="fucP"
                     /locus_tag="OO326_000827"
     CDS             6550..7866
                     /gene="fucP"
                     /locus_tag="OO326_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5965320.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7899..9674
                     /gene="fucI"
                     /locus_tag="OO326_000828"
     CDS             7899..9674
                     /gene="fucI"
                     /locus_tag="OO326_000828"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5965321.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9783..11202
                     /gene="fucK"
                     /locus_tag="OO326_000829"
                     /pseudo
     CDS             9783..11202
                     /gene="fucK"
                     /locus_tag="OO326_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11204..11626
                     /gene="fucU"
                     /locus_tag="OO326_000830"
     CDS             11204..11626
                     /gene="fucU"
                     /locus_tag="OO326_000830"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5965322.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11684..12415
                     /gene="fucR"
                     /locus_tag="OO326_000831"
     CDS             11684..12415
                     /gene="fucR"
                     /locus_tag="OO326_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5965323.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12459..13559)
                     /gene="rlmM"
                     /locus_tag="OO326_000832"
     CDS             complement(12459..13559)
                     /gene="rlmM"
                     /locus_tag="OO326_000832"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5965324.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13552..13947)
                     /locus_tag="OO326_000833"
     CDS             complement(13552..13947)
                     /locus_tag="OO326_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5965325.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13966..14883)
                     /gene="gcvA"
                     /locus_tag="OO326_000834"
     CDS             complement(13966..14883)
                     /gene="gcvA"
                     /locus_tag="OO326_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5965326.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15234..15461)
                     /locus_tag="OO326_000835"
     CDS             complement(15234..15461)
                     /locus_tag="OO326_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5965327.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15653..16858
                     /gene="csdA"
                     /locus_tag="OO326_000836"
     CDS             15653..16858
                     /gene="csdA"
                     /locus_tag="OO326_000836"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5965328.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16858..17301
                     /gene="csdE"
                     /locus_tag="OO326_000837"
     CDS             16858..17301
                     /gene="csdE"
                     /locus_tag="OO326_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5965329.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17352..18158)
                     /gene="tcdA"
                     /locus_tag="OO326_000838"
     CDS             complement(17352..18158)
                     /gene="tcdA"
                     /locus_tag="OO326_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5965330.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18396..19493)
                     /gene="mltA"
                     /locus_tag="OO326_000839"
     CDS             complement(18396..19493)
                     /gene="mltA"
                     /locus_tag="OO326_000839"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5965331.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19702..19778
                     /locus_tag="OO326_000840"
     tRNA            19702..19778
                     /locus_tag="OO326_000840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19736..19738,aa:Met,seq:cat)
     gene            complement(19853..21106)
                     /gene="amiC"
                     /locus_tag="OO326_000841"
     CDS             complement(19853..21106)
                     /gene="amiC"
                     /locus_tag="OO326_000841"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5965332.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21338..22669
                     /gene="argA"
                     /locus_tag="OO326_000842"
     CDS             21338..22669
                     /gene="argA"
                     /locus_tag="OO326_000842"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5965333.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22731..24557)
                     /gene="recD"
                     /locus_tag="OO326_000843"
     CDS             complement(22731..24557)
                     /gene="recD"
                     /locus_tag="OO326_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5965334.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24557..28099)
                     /gene="recB"
                     /locus_tag="OO326_000844"
     CDS             complement(24557..28099)
                     /gene="recB"
                     /locus_tag="OO326_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5965335.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28092..30980)
                     /gene="ptrA"
                     /locus_tag="OO326_000845"
     CDS             complement(28092..30980)
                     /gene="ptrA"
                     /locus_tag="OO326_000845"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5965336.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31156..34524)
                     /gene="recC"
                     /locus_tag="OO326_000846"
     CDS             complement(31156..34524)
                     /gene="recC"
                     /locus_tag="OO326_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5965337.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34537..34896)
                     /locus_tag="OO326_000847"
     CDS             complement(34537..34896)
                     /locus_tag="OO326_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5965338.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVLMVMIVTALSG
                     IQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVS
                     PGGREGEMTRLHCPNCQ"
     gene            complement(34881..35288)
                     /locus_tag="OO326_000848"
     CDS             complement(34881..35288)
                     /locus_tag="OO326_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5965339.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35285..35848)
                     /locus_tag="OO326_000849"
     CDS             complement(35285..35848)
                     /locus_tag="OO326_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5965340.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35839..36309)
                     /locus_tag="OO326_000850"
                     /pseudo
     CDS             complement(35839..36309)
                     /locus_tag="OO326_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36493..37287)
                     /gene="thyA"
                     /locus_tag="OO326_000851"
     CDS             complement(36493..37287)
                     /gene="thyA"
                     /locus_tag="OO326_000851"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5965341.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37294..38169)
                     /gene="lgt"
                     /locus_tag="OO326_000852"
     CDS             complement(37294..38169)
                     /gene="lgt"
                     /locus_tag="OO326_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5965342.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38320..40566)
                     /gene="ptsP"
                     /locus_tag="OO326_000853"
     CDS             complement(38320..40566)
                     /gene="ptsP"
                     /locus_tag="OO326_000853"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5965343.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40579..41109)
                     /gene="rppH"
                     /locus_tag="OO326_000854"
     CDS             complement(40579..41109)
                     /gene="rppH"
                     /locus_tag="OO326_000854"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5965344.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41794..42483
                     /gene="mutH"
                     /locus_tag="OO326_000855"
     CDS             41794..42483
                     /gene="mutH"
                     /locus_tag="OO326_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5965345.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42552..43265
                     /locus_tag="OO326_000856"
     CDS             42552..43265
                     /locus_tag="OO326_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5965346.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43403..43621
                     /gene="ygdR"
                     /locus_tag="OO326_000857"
     CDS             43403..43621
                     /gene="ygdR"
                     /locus_tag="OO326_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5965347.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43729..44769
                     /locus_tag="OO326_000858"
     CDS             43729..44769
                     /locus_tag="OO326_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5965348.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44802..45992)
                     /gene="lplT"
                     /locus_tag="OO326_000859"
     CDS             complement(44802..45992)
                     /gene="lplT"
                     /locus_tag="OO326_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5965349.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45985..48144)
                     /gene="aas"
                     /locus_tag="OO326_000860"
     CDS             complement(45985..48144)
                     /gene="aas"
                     /locus_tag="OO326_000860"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5965350.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48730..49761
                     /gene="galR"
                     /locus_tag="OO326_000861"
     CDS             48730..49761
                     /gene="galR"
                     /locus_tag="OO326_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5965351.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49768..51030)
                     /gene="lysA"
                     /locus_tag="OO326_000862"
     CDS             complement(49768..51030)
                     /gene="lysA"
                     /locus_tag="OO326_000862"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5965352.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51152..52087
                     /gene="lysR"
                     /locus_tag="OO326_000863"
     CDS             51152..52087
                     /gene="lysR"
                     /locus_tag="OO326_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5965353.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52074..52766)
                     /gene="ygeA"
                     /locus_tag="OO326_000864"
     CDS             complement(52074..52766)
                     /gene="ygeA"
                     /locus_tag="OO326_000864"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5965354.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52895..54313)
                     /gene="araE"
                     /locus_tag="OO326_000865"
     CDS             complement(52895..54313)
                     /gene="araE"
                     /locus_tag="OO326_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5965355.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54628..55389)
                     /gene="kduD"
                     /locus_tag="OO326_000866"
     CDS             complement(54628..55389)
                     /gene="kduD"
                     /locus_tag="OO326_000866"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5965356.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55419..56255)
                     /gene="kduI"
                     /locus_tag="OO326_000867"
     CDS             complement(55419..56255)
                     /gene="kduI"
                     /locus_tag="OO326_000867"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5965357.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56542..57723)
                     /locus_tag="OO326_000868"
     CDS             complement(56542..57723)
                     /locus_tag="OO326_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5965358.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57978..59207
                     /locus_tag="OO326_000869"
                     /pseudo
     CDS             57978..59207
                     /locus_tag="OO326_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14758 a        16040 c        14547 g        14427 t
ORIGIN      
        1 cccggtgacg tttgttgccg ggttttctcg tttttggtca cttactcatc aactcatttc
       61 atttgttata tgaatgtttc ttaccaccct cacgcgacaa atatcatcac agttaatatg
      121 tcatacaatt tatgttgcaa cacaaacgtt tccctatttt cataaaccgt attttatcgc
      181 tatgaaaaag aaattatcgc catgattaac taaaagtatt gattttttca gttcaaccta
      241 catatattgc gcgccccgga agaagtcaga tgtcgtttaa tgggcaaata ttgcccttaa
      301 attctctttt acttttgatt tacagagtaa agcgttggga taatctatct tccaagcaga
      361 ttattgtatt tgggatcaag atcactgata gatacataac ttgtgtgtat ctttccgccc
      421 tcaaattatt acggcggtaa atgattaagc catcgccgat agacagattt catttttacg
      481 gtcaggcacc ttcccgggct gaactggcta aaagctgaat tatttgcatt cctccaggag
      541 aaatagatgg aaacgactca aaccagcacg attgcgtcga aagactctcg tagtgcctgg
      601 cgcaagacag acaccatgtg gatgctgggc ctttacggca cggcaatcgg cgcgggcgtg
      661 ctgttcctgc caatcaacgc cggtgttggc ggtatgatcc cgctgatcat catggctatc
      721 cttgcgttcc cgatgacgtt ttttgctcac cgcggcctga ctcgcttcgt actgtctggt
      781 aaaaacccgg gcgaagacat caccgaggtt gtagaagaac actttggtat tggcgcaggt
      841 aaactgatta ccctgctcta cttcttcgct atctacccga tcctgctggt ttatagcgtg
      901 gcaatcacca ataccgttga aagcttcatg tctcaccagc tgggtatgac gccaccgccg
      961 cgtgcgattc tgtcgctgat cctgatcgtg ggtatgatga ccatcgttcg ctttggtgag
     1021 cagatgatcg ttaaagcgat gagtattctg gtattcccgt ttgttggcgt actgatgctg
     1081 ctggctctgt acctgatccc gcagtggaac ggcgcagcac tggaaacgct gtctctggac
     1141 actgcatctg caaccggaaa cggtctgtgg atgaccctgt ggctggcaat tccggtaatg
     1201 gtgttctcgt tcaaccactc tccgatcatc tcttctttcg ccgttgcgaa gcgtgaagag
     1261 tacggcgata tggcagaaca gaaatgctcg aagatcctgg cattcgcaca catcatgatg
     1321 gtgctgaccg taatgttctt cgtcttcagc tgcgtactga gcctgactcc ggcagacctg
     1381 gctgcggcta aagagcagaa catctcgatt ctgtcttacc tggctaacca ctttaacgca
     1441 ccggttatcg cgtggatggc tccgattatc gcgattatcg ctatcaccaa atccttcctc
     1501 ggccactacc tgggcgcacg tgaaggcttc aacggtatgg tgattaaatc tctgcgtggt
     1561 aaaggtaagt ctatcgaaat caacaagctg aaccgtatca ctgcgctgtt catgctggta
     1621 acgacctgga ttgttgccac cctgaacccg agcatcctgg gtatgattga aaccctgggc
     1681 ggcccaatca tcgcgatgat cctgttcctg atgccgatgt acgcaattca gaaagtaccg
     1741 gcaatgcgta agtacagcgg tcacatcagc aacgtattcg ttgtcgtgat gggtctgatt
     1801 gcaatctccg caatcttcta ctctctgttc agctaagtcc tttcgcgccg ctttcgggcg
     1861 gcgcttcctc cgttttaacg cgatgtattt cctatgatta gcgtattcga tattttcaaa
     1921 atcggcattg gcccttccag ttctcatacc gttggaccaa tgaaagcggg taaacaattt
     1981 accgacgatc tgattgcccg taacctgctt aaagacgtga cccgcgtggt ggttgacgtg
     2041 tacggctcgc tctctctgac tggtaaaggc caccacacag atatcgccat tattatgggc
     2101 ctggcgggta acctgccgga taccgtggat atcgattcca tccccggttt tattcaggat
     2161 gtgaatactc atggtcgcct gatgctggca aacggtcagc atgaagtgga gttcccggtt
     2221 gatcagtgca tgaacttcca tgcagacaac ctttctctgc atgaaaacgg tatgcgcatt
     2281 accgcgctgg cgggcgataa agtcgtttac agccagactt actactctat tggcggtggc
     2341 tttatcgttg atgaagagca ttttggccag caggatagcg caccggttga agttccttat
     2401 ccgtacagtt cagcagccga tctgcaaaaa cattgtcagg aaaccgggct gtcactctct
     2461 ggcctgatga tgaaaaacga gctggcgctg cacagtaaag aagagctgga acagcacctg
     2521 gcgaacgtct gggaagtcat gcgtggcggt attgagcgcg gtatttccac cgaaggcgtg
     2581 ttgcctggca aactgcgcgt tccacgccgt gctgcggcac tacgccggat gctggtcagc
     2641 caggataaaa ccaccactga cccgatggcg gttgttgact ggatcaacat gtttgcactg
     2701 gcagtgaacg aagagaacgc tgctggcggt cgcgtggtga ctgcgccgac taacggtgcg
     2761 tgcgggatta tcccggctgt gctggcgtac tacgataagt ttatccgcga agtgaacgct
     2821 aactcactgg ctcgttacct gctggtagcc agcgccattg gttctcttta taagatgaac
     2881 gcgtcgattt ctggtgccga agtgggttgc cagggtgaag ttggcgtggc gtgctctatg
     2941 gcggcagctg gtctggcaga actgttaggc gcaagcccgg cgcaggtttg catcgcagcg
     3001 gaaatcgcga tggagcacaa cctcggtctg acttgtgacc cggtcgcggg gcaggttcag
     3061 gtaccgtgca ttgagcgtaa cgccattgca gcagtgaaag cggtgaacgc cgcgcgtatg
     3121 gcactgcgcc gtaccagcga accgcgcgtc tgcctcgata aagttatcga aaccatgtac
     3181 gaaacaggta aagacatgaa cgccaagtac cgcgaaacct ctcgcggcgg cctggcgatg
     3241 aagatcgttg cctgcgatta atctctccca gaggcctcgt tttgcgaagc ctcttcctgt
     3301 tttctcaacc agccatagcc tgttccggca tcgaatgtta cccttatcgc ctgatcttta
     3361 agggggttat cgtggctgtt catttgctta ttgtcgatgc actgaatctt atccgtcgca
     3421 ttcatgccgt tcagggttcg ccctgtgtcg aaacctgcca gcatgcgctc gatcagctca
     3481 ttatgcacag ccagccaacc cacgcggtcg ccgtttttga tgatgaaaac cgcagtagcg
     3541 gctggcgtca tcagcgttta ccagattaca aagcggatcg accgccaatg ccggaagagt
     3601 tgcacgacga gatgcctgca ttacgcgccg cctttgagca acgcggcgtc ccgtgctggt
     3661 cagccagcgg caacgaagcc gatgacttag ccgccacact ggcggtcaaa gtgacacagg
     3721 ccgggcatca ggcaacgatt gtttcgacag ataaaggcta ctgtcagtta ctttcaccga
     3781 cattacgtat tcgtgattac ttccagaaac gttggctgga tgcgccattt atcgataaag
     3841 aatttggcgt tcaaccgcag cagttgcccg attactgggg acttgcgggg atcagcagtt
     3901 caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc cacgcagctg ctggtcgagt
     3961 ttcagagtct ggaagggata tatgagaatc tggatgcggt tgccgaaaag tggcgcaaaa
     4021 aattagaaac ccataaagag atggcgtttc tgtgccgcga tattgcccgc ttacaaaccg
     4081 atttgcatat cgacggcaat ttacagcaat tgcggttggt acggtaacgg cgagccggat
     4141 acgccgcaaa tcgtatccgg cattatcaca tcagcgcatt taccaggcgg tatggtaaag
     4201 ctctacaata tcctcaagcg ttgcttcacg cgggttgcca ccggtacaaa catcatccag
     4261 tgccgcctga gccagtgccg aaatgtcttc cttgcgtaca ccaacatcac gcaaatgtgg
     4321 cggaataccg acatcacggt tgagggcaaa caccgcttca acagcggcat tacgcgcccc
     4381 ttccaggctc ataccttcca ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc
     4441 accggtaaag tcagcgttat agcgcatgac atgcggtaac aggatggcgt tcgcaacacc
     4501 gtgtggagtg ttataaaacg cgcccagtgg atgcgccata ccatgcacca accctaaccc
     4561 aacattcgag aagcccatac ccgcaacata ctgcccgagc gccatttctt ctccggcatc
     4621 cttatcacca gcaaccgatc ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg
     4681 cagtgcatcg gttagtgccc acgcgccacg ggtaatatac ccctcaatag catgagtgag
     4741 cgcatcaaca cccgtcgcgg ctttcagcgc cggaggcata ccatccatca tgtcagcatc
     4801 aataaacgcc acctgcggga tatcatgcgg atcaacgcaa acaaacttgc gccgtttttc
     4861 ttcgtcagtg atcacgtagt taatggtcac ttctgccgca gtacctgctg tggtggggat
     4921 tgccagaatc ggtacactgg gtttattggt cggggaaagc ccttccaggc tacgcacatc
     4981 ggcaaactcc gggttgttgc tgataatgcc aattgcttta caagtatcct gtggagaacc
     5041 accaccaata gcgatcaggt aatccgcgcc gctattctgg aatacaccga gcccttcttt
     5101 gacgacagta attgttgggt tgggcactac gccgtcgtaa atcgcccatg ccagccctgc
     5161 agcatccatc ttatcggtca ctttcgccac cacgccgcat tgcaccagcg ttttatcggt
     5221 gacgatcagc gccttctgat aaccacggcg tttcacctca tcggttaaag ccccaacagc
     5281 accccgacca aaccatgccg tttcgttcag aatcattctg ttagccatca tccttctcct
     5341 tgttgcttta cgaaattact cttcaatccg taacccatag gttttgaatt tctccagcac
     5401 tacggcaatc tcttcatcgc tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag
     5461 gtaaagttgc gccagcactt caacttcatg cgccagccat aacgcttttt ccagattcac
     5521 ctcacaagcg ataagcccat gatgttgtaa caaagttgcc ttacgatttt tgagagccag
     5581 cgcaacatgt tcagaaagtt cgcgtgttcc aaaggtcgca taaggcgcgc aaggaataga
     5641 attaccgcca gccgccgcaa tcatgtagtg aatagcgggg atcgatcggt taagaatgga
     5701 aactgccgtg caatgaacgg catgattgtg aacaaccgcg ttggcatccg gtctgctttg
     5761 ataggctgcc atatggaaac gccattcgct tgaggggagc tttccttcct catgtttacc
     5821 gttgccatca ataaagacaa tatgcgactc cgtcagtttt tcatatggaa tgcctgtagg
     5881 cgtaatcagc atcccatcct gataacgtac actgacgttc cccgctgtcc cctggttcag
     5941 tcccaggcgg gtcatttcca ggcaagtgtc aataatctga cgagcaagtt tatttcgttc
     6001 cattagctac ctctctctga ttcaaaacag ggcaataatg ttgttccttt cacactattg
     6061 aattagccgt ttaattaccc accatcttct tcctgattaa caagaaagaa attcacaagc
     6121 ttatattttg tgacctagct caactaatca cagtaaataa ctgcaagttc tctttttata
     6181 accccgttaa aaatgaccgc tcttaaaaat atttatcaaa acggtcattt ttccatttcc
     6241 cctcgccaga aatgcccgtt ttcggcacaa acaattaata cggtcatctg gtttgtgttt
     6301 tttatgattt attttctgaa acgggcatga aatttcgatt attaaagtga tggtagtcac
     6361 ataaagtcac cttccagcta ataagtgtga ccgccgtcat attacagagc gttttttatt
     6421 tgaaaatgaa tccatgagtt catttcagac aggcaaatat tcaccgatat gaagcccgaa
     6481 ctcgctggtt ttgcactttt gaaaacataa ccgattacgt gcttaagctt ctgaacctaa
     6541 gaggatgcta tgggaaacac atcaatacaa acgcagagtt accgtgcggt agataaagat
     6601 gcagggcaaa gcagaagtta cattattcca ttcgcgctgc tgtgctcact gttttttctt
     6661 tgggcggtag ccaataacct taacgacatt ttattacctc aattccagca ggcttttacg
     6721 ctgacaaatt tccaggctgg cctgatccaa tcggcctttt actttggtta tttcattatc
     6781 ccaatccctg ctgggatatt gatgaaaaaa ctcagttata aagcagggat tattaccgga
     6841 ttatttttat atgccttggg cgctgcatta ttctggcccg ctgcagaaat aatgaactac
     6901 accttatttt tagttggcct atttattatt gcagccggat taggttgtct ggaaactgcc
     6961 gcaaaccctt ttgttacggt attagggccg gaaagcagta gtcacttccg cttaaatctt
     7021 gcgcaaacat ttaactcgtt tggcgcaatt atcgcggttg tctttgggca aagtcttatt
     7081 ttgtctaacg tgccacatca atcgcaagac gttctcgata aaatgtctcc agagcaattg
     7141 agtgcgtata aacacagcct ggtattatcg gtacagacac cttatatgat catcgtggct
     7201 atcgtgttac tggtcgccct gctgatcatg ctgacgaaat tcccggcatt gcagagtgat
     7261 aatcacagtg acgccaaaca aggatcgttc tccgcatcgc tttctcgtct ggcgcgtatt
     7321 cgtcactggc gctgggcggt attagcgcaa ttctgctatg tcggcgcaca aacggcctgc
     7381 tggagctatt tgattcgcta cgctgtagaa gaaattctag gtatgactgc aggctttgcc
     7441 gctaactatt taaccggaac catggtgtgc ttctttattg gtcgtttcac cggtacctgg
     7501 ctcatcagtc gcttcgcacc acacaaagtc ctggccgcct acgcattaat cgctatggca
     7561 ctgtgcctga tctcagcctt cgctggcggt catgtgggct taatagccct gactttatgc
     7621 agcgccttta tgtcgattca gtacccaaca atcttctcgc tgggcattaa gaatctcggc
     7681 caggacacca aatacggttc gtccttcatc gttatgacca ttattggcgg cggtattgtc
     7741 actccggtca tgggttttgt cagtgacgcg gcgggcaaca tccccactgc tgaactgatc
     7801 cccgcactct gcttcgcggt catctttatc tttgcccgtt tccgttctca aacggcaact
     7861 aactgaacat attttccgaa taaagtgagg aatctgtaat gaaaaaaatc agcttaccga
     7921 aaattggtat ccgcccggtt attgacggtc gtcgcatggg tgttcgtgag tcgcttgaag
     7981 aacaaacaat gaatatggcg aaagctacgg ccgcactgct gaccgagaaa ctgcgccatg
     8041 cctgcggagc tgccgtcgag tgtgtcattt ccgatacctg tatcgcgggt atggctgaag
     8101 ccgctgcttg cgaagaaaaa ttcagcagcc agaatgtagg cctcaccatt acggtaacgc
     8161 cttgctggtg ctatggcagt gaaaccatcg acatggatcc aacccgcccg aaagccattt
     8221 ggggctttaa cggcactgaa cgccccggcg ctgtttacct ggctgcggct ctggcagctc
     8281 acagccagaa aggcatcccg gcattctcca tttacggtca tgacgttcag gatgccgatg
     8341 acacatcgat tcctgccgat gttgaagaaa aactgctgcg ctttgcccgc gccggtttgg
     8401 ccgtcgccag catgaaaggt aaaagctatc tgtcgctggg cggcgtttcg atgggtatcg
     8461 ccggttctat tgttgatcac aacttctttg aatcctggct gggaatgaaa gtccaggcgg
     8521 tggatatgac cgaactgcgt cgccgtatcg atcagaagat ttacgacgaa gccgaactgg
     8581 aaatggcact ggcctgggct gataaaaact tccgctatgg cgaagatgaa aataacaaac
     8641 agtatcaacg caatgccgag caaagccgcg cagttctgcg cgaaagttta ctgatggcga
     8701 tgtgtatccg cgacatgatg caaggcaaca gcaaactggc cgatattggt cgcgtggaag
     8761 aatcacttgg ctacaacgcc atcgctgcgg gcttccaggg gcaacgtcac tggaccgatc
     8821 aatatcccaa tggtgacacc gccgaagcga tcctcaacag ttcatttgac tggaatggcg
     8881 tgcgcgaacc ctttgtcgtg gcgaccgaaa acgacagtct taacggcgtg gcaatgctaa
     8941 tgggtcacca gctcaccggc accgctcagg tatttgccga tgtgcgtacc tactggtcac
     9001 cagaagcaat tgagcgtgta acggggcata aactggacgg actggcagaa cacggcatca
     9061 tccatttgat caactcaggt tctgctgcgc tggacggttc ctgtaaacaa cgcgacagcg
     9121 aaggtaaccc gacgatgaag ccgcactggg aaatctctca gcaagaggct gacgcttgcc
     9181 tcgccgctac cgaatggtgc ccggcgatcc acgaatactt ccgtggcggc ggttactctt
     9241 cccgcttcct taccgaaggc ggcgtcccgt tcaccatgac tcgtgtcaac atcatcaaag
     9301 gcctgggacc ggtactgcaa atcgcggaag gctggagcgt ggaattgccg aaggatgtgc
     9361 atgacatcct caacaaacgc accaactcaa cctggccaac cacctggttt gcaccgcgcc
     9421 tcactggtaa agggccgttt acggatgtgt attcggtaat ggcgaactgg ggtgccaacc
     9481 acggagttct gaccatcggc cacgttggcg cagactttat cacccttgcc tccatgctgc
     9541 gtatcccggt atgtatgcac aacgttgaag agaccaaagt gtatcgtcct tctgcctggg
     9601 ctgcgcacgg catggatatt gaaggccagg attaccgcgc ttgccagaac tacggtccgt
     9661 tgtacaagcg ttaatacatt acaccctgat gtgatgcccg gtcgctccgg ctaccgggcc
     9721 tgaacaagca agagtggtta gccggataaa caatgttatc cggctatatt gcaggagcga
     9781 ttatgaaaca agaagttatc ctggtactcg actgtggcgc gaccaatgtc agggccatcg
     9841 cggttaatcg gcagggaaaa attgttgccc gcgcctcaac gcctaatgcc agcgatatcg
     9901 cgatggaaaa caacacctgg caccagtggt ctttagactg ccattttgca acgctttgct
     9961 gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc acatccgcgg tatcgccgtc
    10021 accaccttta gtgtggatgg cgctctggta gataagcaag gcaatctgct ctatccgatc
    10081 attagctgga aatgtccgcg aacagcagcg gttatggaca atattgaacg gttaatctcc
    10141 gcacagcggt tgcaggctat ttctggcgtc ggagccttta gtttcaatac gttatataag
    10201 ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac gcgcgcacgc ctggctcttc
    10261 atttcgtcgc tgattaacca ccgtttaacc agcgaattca ctactgatat cacgatggcc
    10321 ggaaccagcc agatgctgga tatccagcaa cgcgatttca gtccgcaaat tttacaagcc
    10381 accggtattc cacgccgact cttccctcgt ctggtggaag cgggtgaaca gattggtacg
    10441 ctacagaaca acgccgcagc aatgctcggc ttacccgttg gcataccggt gatttccgca
    10501 ggtcacgata cccagttcgc cctttttggc gctggtgccg aacaaaatga acccgtgctc
    10561 tcctccggta catgggaaat tttaatggtt cgcagcgcct aggttgatac ttcgctgtta
    10621 agtcagtacg ccggttccac ctgcgaacta gatagccagg catggttgta taacccaggt
    10681 atgcaatggc tggcatccgg cgtgctggaa tgggtgagaa aactgttctg gacggctgaa
    10741 acaccctggc aaatgttgat tgaagaagct cgtctgatcg cacctggcgc ggatggtgtg
    10801 aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct ggcaaggagt gacgcttaat
    10861 accacgcggg ggcatttcta tcgcgcggcg ctggaagggt taactgcgca attacagcgc
    10921 aatctacaga tgctggaaaa aatcgggcac tttaaagcct atgaattatt gttagtcggt
    10981 ggaggaagtc gcaacacatt gtggaatcag attaaagcca atatgcttga tattccggta
    11041 aaagttctcg acgatgccga aacgaccgtc gcaggagctg cgctgttcgg ttggtatggc
    11101 gtaggggaat ttaacagccc ggaagaagcc cgtgcacaga ttcattatca gtaccgttat
    11161 ttctacccgc aaactgaacc tgaatttata gaggaagtgt gaaatgctga aaacaatttc
    11221 gccgttaatt tctcccgaac tattgaaagt gctggcagag atgggacatg gagatgaaat
    11281 tattttttcc gatgctcact ttcccgccca ttcgatggaa ccgcaggtga tccgcgctga
    11341 tggcctgttg gtgagcgact tgctccaggc gattatcccg ttatttgagc tggacagtta
    11401 tgccccgccg ctggtgatga tggcggcggt agagggtgac actctcgatc ctgaagtaga
    11461 acgacgttac cgtaatgcgc tttcactaca agccccgtgt cctgacatca tccgcatcaa
    11521 tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg atcgttatca caggcgaacg
    11581 agcgaagtac gggaatattc ttttaaaaaa aggggtaaca ccgtaatctc ataccggtac
    11641 gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa aatatgaaag cggcacgcca
    11701 gcaagcgata gtcgacctgc tgctgaacca taccagcctg accacggaag ctctctctga
    11761 acagctaaag gtcagtaaag aaaccattcg tcgcgatctc aatgaattac agacgcaggg
    11821 taaaattctg cgcaatcatg gacgcgctaa atatatccac cgtcaaaatc aagacagtgg
    11881 cgatcccttt cacatcaggc tgaaaagcca ttatgcgcat aaagcagata tcgcgcgcga
    11941 ggcgctcgcg tggattgaag aagggatggt gatagcctta gacgccagtt caacttgctg
    12001 gtatctggca cgccagttgc ctgacatcaa cattcaggtc ttcaccaata gccatccgat
    12061 ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc agttccggcg gcacacttga
    12121 gcgcaaatat ggctgttacg tcaatccctc gctgatttcc caacttaaat cgctggaaat
    12181 cgatctgttt attttttctt gtgaagggat cgatagcagc ggcgcactgt gggactccaa
    12241 tgcgatcaac gctgattaca aatcgatgct attaaaacgt gccgcgcaat cgttgttatt
    12301 gattgataaa agtaaattta atcgttcagg ggaagcccgc atcgggcatc tggatgaggt
    12361 aacgcacatt atttctgatg agcgccaggt tgcaacttct ttggtaacag cctgacggaa
    12421 agggtagcag gccggagacg acgccccggc cttgcctgtt atcgctcgtc gcgacgacca
    12481 cccaccgcag cccagatgcg gcggacgtgc accgtcactt cttcgcgatc gtgatacaac
    12541 tgccgcgcct gaatctgagc atttatgcca tgttcatcaa gctgtgcctg aatatacgcc
    12601 agattgtgtg atacttcttc gtagcgtttt ttcatcggca gtttgagatt gaaaatggtt
    12661 tcacggcacc agccattaac cagccactgc gccatcaatg ccgcaacttt agccggtttt
    12721 tcaaccatat cgcataccat ccaggagata ttgctgcgtg tcggacggaa tttgaaaccg
    12781 tcttcccgca gccacgtcac ctgcccggta tccatcagac tttgcgccat cgggccgttg
    12841 tcgacggaat aaacccacat gttgcgcttc accagttggt aggtccagcc gccagggcaa
    12901 gcgcctaaat ccaccgccca catcccgttc gccaggcgtt catcccactc atccgcagga
    12961 ataaacacat gaaatgcctc ttccagtttg agcgtggaac gactcggcgc ctctgccgga
    13021 aatttcaggc gcggaatgcc catatagaac ggcgaattat tgttgctgta tgagtaaccg
    13081 gtatagcagc atcctggtgc aataaagaat acatgcacaa ccggacgctt cggcgtttca
    13141 tagttcgcca gcaccccagc atcgcgcaag gcagcgcgta gcggaacagt gaacttacgg
    13201 cagaatttca gcaactcttt gctttcgttg gtatcggcaa cttcaacacg cagttcaccg
    13261 cccttctcta ctacaccctg taacatgccg acaatggggg taatacgatc ttctggcggc
    13321 aaatgctgca ggagttcccc caccacaaac cactggcggg caaaaattaa tgaactgaac
    13381 ggcagctcac ggattaactt atcgccatca tcaggttgat aacattcata aatgacataa
    13441 cccgcattct ctttcacgcg ggcaaaaccg aaaatttccc gctggccggc tttatcggta
    13501 atttctgcgg cgcactcttt ttcaaagccc ggacggcaca gcaatacaac cttattcatg
    13561 acttacgccc ttgcgcttta aacggatagc accaactaac attaacgccc agcccgcaag
    13621 gaagctcacg ccgccaaccg gtgtgacaaa cgcccacaaa cgcagatggg acagcgccag
    13681 gcaataaagg ctgccgctga acagcaccgt gcctaacgcg aggaaaacgc tactccagta
    13741 aaaccagata ctgatgcgac gctgcattgc caccgccaga cctaagatcg ccagcgtatg
    13801 aaacgcctgg tattcgagac cggtttggat ccagcccatc tcaacggccc ccatggtttt
    13861 acttaacaca tgcgcaccaa aagcgcccag tgccacaaaa atgaagccgc taatggcggc
    13921 gaaaatcagc ataaaacggc tggtcatggt cgtaccctac gtaaattatt gttcataacg
    13981 aaagcggaat ttttcttgtt cagcagcggc tttcgccagg atccattggc gaaaggcggc
    14041 tattttaccc agttctgcct ggctgtcatg acaaaccaga taaaaagcat ttttactgac
    14101 cagaacatca ttaaacgggc aaacaagacg tccggcctcg atttcagatt gcgccatcac
    14161 gttatttgcc agcgccactc cctgcccgtg aatagccgct tgcagcacca tggcgctatg
    14221 actaaaaatt ggcccttgct gaacgttgat atgattaaac cccaactgtc gggtatatgt
    14281 ctgccagtca cggcgtgacg catcatgtaa taacgtatgt ttagccagat cttccggggt
    14341 cttcaagggt ttttcgcctg tcagcagtag cggcgaacac accggcaata aatattcggc
    14401 gtacagtttt tccacccgta gccccggcca gttgccccga ccataaaata tcgccacatc
    14461 aacatcatcc gccagcttat cttcctgacg atcaaccgcc tggattcgaa cgtcaattcc
    14521 cggataagct gaattaaagc tggaaagtcg cggaaccaac caatgaatgg cgaaactggg
    14581 gagtaaactg accgtcaacg cccccttggc gctacgggcc tggagtttac gcgtcgcttc
    14641 ggttaattgc gaaaatatct ctttgatatc gaggaaatag ctttgccctt cctcggtcag
    14701 caggagtgaa cgattacggc ggcggaacag ttttagcccc aaaaaatcct caagagactt
    14761 gatttgatga cttactgcgg cctgggtcac aaaaagctct tctgctgcgc gagtaaaact
    14821 taaatggcgt gctgcggcat caaaaactcg taaggcattt agcggtggta atcgtttaga
    14881 catggctttt aaactttgat gttaaatgaa tttaacaatt agatcacact gtgtaaccta
    14941 ttagtttttt taatctgagc cattataaat tgtccgttga gcttctacca gcaaatacct
    15001 atagtggcgg cacttcctga gccggaacga aaagttttat cggaatgcgt attctggtga
    15061 acttttggct tacggttgtg atgttgtgtt gttgtgtttg caattggtct gcgattcaga
    15121 ccatggtagc aaagctacct tttttcactt cctgtacatt taccctgtct gtccatagtg
    15181 attaatgtag caccgcctaa ttgcggtgct tttttttacc ttgcgatcgc gaattactgt
    15241 tccagttcga ccatctcttt cacgtcagta cggttgatct gctgtttgtt gccattagcg
    15301 tctttatacg aaatcatacc gatatcgtta tcagtctgtg gtttgccgtc agagacgatg
    15361 gtacgtccgt cattggtgtg catcacatag ttcgaaccgg aacaggcgct cagggcaaaa
    15421 gtcagcatac aggcagaaat aattgcggca gtctttttca tcatcttctc cttaaaacga
    15481 ttaataaatc ttaatagtat tgattaacag gctaaaatta acgcctaaca ctattcagca
    15541 tatgttactt acgcggcttc gccaggatat ccagataatt ctgatggtta gcactctcct
    15601 tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag ccgaggagta ccatgaacgt
    15661 ttttaatccc gcgcagtttc gcgcccagtt tcccgcacta caggatgcgg gcgtctatct
    15721 cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt gaagccactc gacagtttta
    15781 cagcctgagc gccggaaacg tccatcgcag ccagtttgcc gaagcccaac gcctgaccgc
    15841 gcgttatgaa gctgcacgag agaaagtggc gcaattactg aatgcaccgg atgataaaac
    15901 tatcgtctgg acgcgcggca ccactgaatc catcaacatg gtggcacaat gctatgcgcg
    15961 tccgcgtctg caaccgggcg atgagattat tgtcagcgtg gcagaacacc acgctaacct
    16021 cgtcccctgg ctgatggtcg cccaacaaac tagagccaaa gtggtgaaat tgccgcttaa
    16081 tgcgcagcga ctgccggatg tcgatttgtt gccagagctg attactcccc gtagtcggat
    16141 tctggcgttg ggtcagatgt cgaacgtgac tggcggttgc ccggatctgg cgcgagcgat
    16201 tacctttgct cattcagccg ggatggtggt gatggttgat ggtgctcagg ggacggtgca
    16261 tttccccgcg gatgttcagc aactggatat cgatttctat gctttctctg gtcacaaact
    16321 gtatggcccg acgggtatcg gcgtgctgta tggcaaatca gaactgctgg aagcgatgtc
    16381 gccctggctg ggtggcggca aaatgattca cgaagtgagt tttgacggtt tcaccactca
    16441 atctgcgccg tggaaactgg aagcaggaac gccaaatgtc gctggcgtca taggattaag
    16501 cgcggcgctg gaatggctgg cagattacga tatcaaccag gccgaaagct ggagccgtag
    16561 cttagcaacg cttgcagaag aagcgctggc gaaacgtcca ggctttcgtt cattccgctg
    16621 ccaggattcc agcctgctgg cctttgattt tgctggcgtt catcacagcg atatggtgac
    16681 gctactggcg gagtacggta ttgccttgcg ggccgggcaa cattgcgctc agccgctact
    16741 ggcagaatta ggcgtaaccg gcacactgcg cgcctcattt gcgccatata atacaaagag
    16801 tgatgtggat gcgctggtga atgccgttga ccgcgcgctg gaattattgg tggattaatg
    16861 acaaacccgc aattcgccgg acatccgttc ggcacaaccg taaccgcaga aacgttacgc
    16921 aataccttcg caccgttgtc gcaatgggaa gataaatatc gccagttgat catgctgggg
    16981 aaacagcttc cggctttgcc agacgagtta aaagcgcagg ctaaagagat tgccggatgc
    17041 gaaaaccgcg tctggctggg atataccgtg gctgaaaacg gcaaaatgca cttctttggc
    17101 aacagcgaag ggcgcattgt gcgcggcctg ttggcggtgt tgttgactgc cgttgagggg
    17161 aaaaccgccg ccgaattaca ggcacagtcg ccgctggcat tgtttgatga gctgggatta
    17221 cgtgcgcagc ttagcgcctc acgcagccag gggttaaatg cgttaagcga ggcgattatc
    17281 gctgcgacga agcaggttta agcacagaac cgggtcggat aagacgttcg cgtctcatcc
    17341 gacctgattg tttaaccctg acgcgccgct ttcgccatca tcttcttcag cgcatgagaa
    17401 accgcaacaa acccaaaggt ggcggtcacc atcgttgccg cgccaaatcc agatgcacaa
    17461 tccatccgct tcggcccttc tgccgtggct ttcatcgcac acaccgtacc gtctgactgc
    17521 gggtacacca gcgcttcagt agaaaacacg caatccacgc cgagcttacc tttactgttt
    17581 ttcactacgc caaaatcgct tttcaggcgc tcgcgcaact tcgccgccag cggatcctga
    17641 atcgttttcg ccagatcggt aacctgaatc tgcgtcgggt caatctgccc acccgcgcca
    17701 ccggtcgtga ccagcgggat tttattgcgc cgacaataag caatcagcgc cgctttgggc
    17761 cgtacactat caatggcatc aatcacgtac gaataaccgg cactcatata ttgcgctacg
    17821 ttatccggcg tcacgaaatc atccaccacc gttacacggc actccgggtt aatctggcga
    17881 atgcgctccg ccatgacttc cgcttttgcc agcccgacgt tatcgcgcag agcgtgaatt
    17941 tgccgattgg tattggtgac gcacacatca tccatatcga taagcgtgat tgcgccaatc
    18001 cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac caccgatacc aaccacacaa
    18061 atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat acagacgcgc tgtgccacca
    18121 aaacgctgac gccatgcatc actaattacc acagacatag cacctcagaa ttaaaaaggg
    18181 cgaagttatc cctcacccta aacgcataaa tgcctgatgc gctacgctta tcaggcctac
    18241 ataactcctg caatattttg gatttgcatg atttgtaggc cggataaggc gttcacgccg
    18301 catccggtat gaacaacgcg cacttagtca ataatcagga tgtgaggtta cccctcccct
    18361 gtcatatccg taaaaacggc atacagaata tcacatcagc cgctaaagac gttacctgcg
    18421 cccggagccg ttttcagcac ccaaacccgt ccatagtggt tgtaccaacc tgcgcggtgt
    18481 ccggcttccg gcccgatccc ttgatagata tcgaagtgtt ggcctttgat tgcgcccccg
    18541 acatccaacg ccaccatcag acgcaattcg tactgaccgt taaatttgcc gttattatcc
    18601 agcagcggca cttctgccag caaggtagta cctggcggaa taattgaacg atcggaggca
    18661 acagaagcac ggccaaccag cggcaccgca cttgcccctt tcaccggtgc aaaagattgc
    18721 ggtttaaaga agacgaaaga cgggttctgt tccagcagct cgcgaacctc ggcttcactg
    18781 tgtgtttcgc cccaatgacg aatcgcctgc atcgacatat cttctttttt cacttcgcca
    18841 cggtcgatca gcaccttacc aatgctgcga taggcatgac cgtttttccc tgcatagctg
    18901 aaaaagttaa gcggactgcc atcaccaaag tcgatatacc cactaccctg cacatccata
    18961 atgaagttat ccatcagaga gttgctgtaa gcgaggatgt atttatcgcc caatgccccg
    19021 gcgtagatct ccgcacgaga cggcagacga ccacgttttg gcggcatacg gtaaatagga
    19081 tactggaact cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta atagcctgta
    19141 aactgcacgt taccatagtt gtcggcacct tccatctgcc aggcatcaat gccgaactgg
    19201 cgcatattgc gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt ataaacatta
    19261 ctctggttgc catacagacg cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa
    19321 tcaccggcgt taatcggcgc gccaacggca tctggctggt tcaccagaga gaaaggctgg
    19381 gtaaatttcc cgtctttata ttgctgtccg cgatcggttg gtttggaaga gcaggcggca
    19441 agcattgcca caaccgtgcc cataagaagg tactttaccc aacgtccttt cattgttctc
    19501 ttcttcaggt taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg
    19561 agcagcctct ccctgacgca aattttgcac aaaaaatagg ctttagtggt ttgtttttgt
    19621 tcaaaatcat gctaaatccg tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg
    19681 agtatagtgc gcatccacgg acgcggggtg gagcagcctg gtagctcgtc gggctcataa
    19741 cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat tattgaacac cctaacgggt
    19801 gtttttttgt ttctgatctc ccataaaaaa agcgccattc agcgcctttt tatcatcccc
    19861 ttctcgccag cgttgcccca tcggcaaaat acgctttaat ccccgcaaga atagactccg
    19921 caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa
    19981 acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct
    20041 gttcaacttg atttttatgc agcttgttga tttttccgag cttattcagt accgctttac
    20101 caaacttaag gctgtcagca atagtcagcg actgcaccat atcgaacatg gtgtggtcga
    20161 catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtttgtg
    20221 ccagatattt tgccgcagta ctggttgcgc ctttggttga gagcgcaaac accgaggaac
    20281 cgctcggctg acgactggta aaggcgtcgg catggataga gacaaacagg tcagcacgct
    20341 gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca
    20401 tgtacacctt catattgccc tcttcctcga tcagagagcg caaacggcga gctatttgca
    20461 ataccacatc tttttcgcgc gttttgtatt tccccaccgc accggagtct tcgccaccgt
    20521 ggccagggtc aagcataatg acaatcggac gatcgcgccc tgctttaccc ggttgtggac
    20581 cactttgcgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg
    20641 ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct
    20701 ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt
    20761 caaaaaccat gcgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg
    20821 ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt
    20881 cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat ttcagctgac
    20941 gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga
    21001 cctggctaac cgcagccagg ctgacctgac ttacgctcaa tagccacata gcgcccgcgc
    21061 cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca
    21121 aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag
    21181 acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga
    21241 taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa
    21301 tacaataatt tcaaataatc atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga
    21361 gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt
    21421 tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga
    21481 tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca
    21541 gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt
    21601 gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat
    21661 tactgctcgc ctgtcaatga gtctcaataa cacgccgctg cagggcgcgc atatcaacgt
    21721 cgttagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg
    21781 ccatagcggg cgtatccggc ggattgatga agacgcgctc catcgtcaac tggaaagcgg
    21841 tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac
    21901 ctcggaagag attgccactc aactagccat caaactgaaa gctgaaaaga tgattggttt
    21961 ttgctcttcc cagggcgtta ctaatgacga cggtgatatt gtctccgaac ttttccctaa
    22021 cgaagcgcaa gcacgggtag aagcccagga agagaaaggc gattacaact ccggtacggt
    22081 gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgccgct gtcatttaat
    22141 cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac
    22201 gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg
    22261 tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga
    22321 gcagctggag atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc
    22381 ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt
    22441 tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca ttgccgctca
    22501 ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt
    22561 ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca aaaagcagtt
    22621 gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat
    22681 caccggatgc ggcaggcgta acactcgtgc gtcgcatccg gcaattacgt ttattcccgc
    22741 gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt
    22801 aatatacgct catcggcata cagcgacaga cgacgacgcg cgcgggtcac cgcggtgtat
    22861 accagctctc gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg
    22921 aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag
    22981 cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc
    23041 tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta
    23101 cgggcaatca tcaccggtcg gccttcgtac caacgagagt gcggatgacg atgaattttg
    23161 cgcttctgtt gcataaactg ttcaattcgc tcattcagtc cagccacgcc aaacggccct
    23221 tcccgcaggg cgcacaaaag ctggtactca ttgaacgcct gaatgattaa atccggctcg
    23281 gcacgcgctt gcagcagatc cagataacgt ccgtaacccg caagagcttc ctcaagcatc
    23341 gcaatataat cttcgccgct ctgtaaaagt cgtttttcga tatcagtaaa atcctgctga
    23401 aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg
    23461 ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat
    23521 gccgcttctg tgccggttcc tgccggaacg tgagtccccg tcaggcggct tagctgcctg
    23581 gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga tatcgcccag cacagcccca
    23641 gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatctggc
    23701 aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc
    23761 agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc
    23821 gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtttctt ttgttcatcg
    23881 gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta
    23941 cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct
    24001 gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag
    24061 atccgccgcg tcagcgccac tgccgctgca actttttgcc agttaatttc atcgcttact
    24121 ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat ggtatgattc
    24181 acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga
    24241 cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc
    24301 agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc
    24361 ggattcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg
    24421 gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg
    24481 gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat
    24541 tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca
    24601 gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat
    24661 caacgccacg caggaacaga tagataacgc cgccaaagtg aagatcatag tcgtaatcag
    24721 caatgcgatg gcgcagataa cgatgcagcg ccagggtata aagctgatat tgcagatcat
    24781 agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac
    24841 ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt
    24901 cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg
    24961 atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa
    25021 tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa
    25081 cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt
    25141 cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg
    25201 gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc
    25261 gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat
    25321 caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg
    25381 agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt
    25441 ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc
    25501 aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg
    25561 gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt
    25621 tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg
    25681 tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga
    25741 cgctttctgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa
    25801 acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca
    25861 gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt tcgaggcgca
    25921 tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca
    25981 gcgcatgttc actttccagc tgcgttccgg cttcttgaag cagttcgctg atatgcaaga
    26041 tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca atgttacgcg
    26101 ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac
    26161 catcgaactc ttcgactacc gcatcccacg catgttcgtc attattcagc gtttcaatat
    26221 ccagcgcatt cagccccatc attgacgttg ccagcgcact gcgcagggtg ttctcacgtt
    26281 cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa
    26341 cactgtcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag gcatcgcgca
    26401 cctgggcggc ctcctggcgg ctgcgcacca gcacgctgat gtccgaagca cgcaccggac
    26461 gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc
    26521 ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt
    26581 cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaat acaaaacgta
    26641 acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg
    26701 tgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt gcggagcgcc
    26761 agttggtgtc taaagtgtag tgggcatgaa cttcactacg cgccttcata taagtgaaga
    26821 tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc aacaatgcgg
    26881 tttccggctg atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct
    26941 gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcgc
    27001 cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc
    27061 cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggtgatca
    27121 acagatcgcg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca aacagtggat
    27181 gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa gaaacgctgg gagaattttt
    27241 ccagcgactc cggcaactga taactgtttg tctcttcttc tgcccaggcg ctgatctttt
    27301 cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga
    27361 tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg
    27421 ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt gctttgataa
    27481 ccggcggttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc
    27541 aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg cgacgccaga
    27601 aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca
    27661 gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaagccg tgaatagtaa
    27721 agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt
    27781 tatcgtcgat ctcttccagc aggcgttcgt acagtggatt gtcggtggtt tcacgcagac
    27841 aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag
    27901 cctcggtaaa ggtgaccacc agcagttctt caacgatcag cgggcgggga aaggcggcgg
    27961 aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg
    28021 tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg
    28081 tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg
    28141 acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg
    28201 ccggaaatct gcgacagaat agccatgcct tgcggctcga ccaccgcctg atggaagaaa
    28261 tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg
    28321 aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt
    28381 tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc
    28441 ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag
    28501 aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg
    28561 ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag
    28621 aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg
    28681 tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca
    28741 aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca
    28801 tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg
    28861 ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc
    28921 acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc
    28981 acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg
    29041 gaatccatca tctggttata ccaggacttc gcctgctcaa gctgctcttc cgtagcggta
    29101 tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca
    29161 ttagcattaa tcataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac
    29221 gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc
    29281 acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct
    29341 ttgggctcgc tggcaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca
    29401 acaatcagct ctggatggtc gtatttcttc tctgacttaa tcagcgagaa atcatcagga
    29461 atataagggt taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtcg
    29521 gcgaaagttt gtgcgctgat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt
    29581 ttgttgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc
    29641 gccagacgtt cctttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc
    29701 gtatgctcaa caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca
    29761 cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat
    29821 tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc cgccacaacc
    29881 tgatcgcgat tagccaggcc tttatcagtt aaagacgcag agatcgctaa tacgccgctg
    29941 ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc
    30001 agccagtcag aaagtgtacc tgggctgcga ttgccaatca gataggtaat caattcatcg
    30061 gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg caacacttta
    30121 cgcggcagcg cagggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc
    30181 acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc
    30241 atttttgcca actccggcag cggtttatta ctgtaaataa ccgccttcat caaattggcg
    30301 gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg
    30361 cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt
    30421 tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg
    30481 ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca
    30541 tcggccaggc ggtctaccgc accgggcaag gcgtcgttct caacttccag atagaaagcc
    30601 gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc
    30661 agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt
    30721 gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcactac cagcgccgag
    30781 agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt
    30841 atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc
    30901 catcccgttt ctgcctgact taatggtgcc cagagggcaa caaacaacaa taatgctttg
    30961 aaccaggtgc tgcggggcat tcacggacct cataatcaac tttattttct ggacagattc
    31021 aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg
    31081 tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcagat agattgcgca
    31141 atttttatac aacactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga
    31201 ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc gttgatacca
    31261 gatatcatca ccttcgccac gcaccatcat gttgccttcg taagcctgaa ggaatttcgt
    31321 acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca
    31381 ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg acattccttc
    31441 acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg
    31501 aaaacgccac tcaccgtctt tgcgtagaaa aaggcgactt tcgccattac caccgctggc
    31561 acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg
    31621 acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc
    31681 gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc
    31741 ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata
    31801 cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac
    31861 cagtgcattc aataactgct gattgatttg ataacggcta agtccttcca gaataaatgg
    31921 ctcggtgtct gggatttcgc tgtcttcagt atggaagttc acctgcaaac gcatctggaa
    31981 gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg
    32041 taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc
    32101 aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gcggatcaaa
    32161 cggcatccgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca
    32221 gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg
    32281 caccagtacc gacgggaaac gttcactgtt atcctgaatg gaacgcccga tatagctgat
    32341 atagagtttt tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt cgtcatcgcg
    32401 acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag
    32461 ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga
    32521 acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg
    32581 ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc
    32641 atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat
    32701 cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg
    32761 caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat
    32821 tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg
    32881 tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca acatacgcgt
    32941 caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc
    33001 gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc
    33061 ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagcagcg ccagcacatc
    33121 ctctgacaca aagcgactgt caggcagtga taacaggctg ataaacgctt ccagtaccgg
    33181 atgtgactgc cgcgcacgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc
    33241 gctaccaaac actgcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat
    33301 gatgtcgcgc ggagtaagtg tcggggcttc ctccagcatc gccagcaagc gatcgtgtaa
    33361 aacttcaact tcacgctgcg ggctatggca aacgtggaat gtgatactgc tatccagtgg
    33421 atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac ccgcaacggc
    33481 gcggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtcac
    33541 atcgacaaaa gcgtccagct cctggctgct ctccaggtca gaaaggagat aaatgtagtc
    33601 gcgcccaagc ttcccccatg aggccagcag cgagttgccg acatcctgtt caccatcgct
    33661 gttaaagagc tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc gatcttcaaa
    33721 actgtgtcga cgctgacgag tcagcagttt cgccagataa gcaggatctt taatatcgcc
    33781 ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc
    33841 ctggagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga
    33901 aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag
    33961 attcgcgcgg tgccagcgcg gttgtccgag ttcatgggta tattccacca gcgccttcca
    34021 caacggggct tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca
    34081 ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga
    34141 ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt
    34201 aaagtcttcg cgctccagca attgcggcag cagagtcatc agtttccagc tcatgctctg
    34261 tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat
    34321 aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt
    34381 catttgcagc cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg
    34441 atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg
    34501 attggaatgg tagaccctta acatagcggc tcctgactac tgacaattcg ggcaatgcag
    34561 gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca
    34621 tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg
    34681 cagttgcgtt tgctgccaac catgccgcca gagcttttgg tactggtttc tgctggcgag
    34741 actgttcatt gatgttcgct ggatacccga taacgcagtg acaatcatca ccatcagcac
    34801 catcagcacc atcagcacca tcagcaccat cagcaccatc gcaaccatta cctccggcag
    34861 gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt cagcggacaa
    34921 aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc gcctgttcgc
    34981 cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc cagtaaacgc
    35041 aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc
    35101 caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac
    35161 tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat tccttgcaat
    35221 agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac acctttttcg
    35281 cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt tgcggttcag
    35341 acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgac
    35401 gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct
    35461 taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc
    35521 caatctggtc ggactctttt accggttcgc gatcccagat accgttactg ttcgcatccc
    35581 actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc
    35641 catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt
    35701 catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac gcaggcagaa
    35761 agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca
    35821 gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat aaagccggag
    35881 tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg
    35941 cacggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat
    36001 ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc
    36061 cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg
    36121 gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctagct
    36181 ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc
    36241 gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg
    36301 tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc
    36361 ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc
    36421 gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg gctctggcag
    36481 gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat cgtagccttc
    36541 aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa
    36601 cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatggttgct
    36661 gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg
    36721 cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac
    36781 gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa
    36841 gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac
    36901 aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag taatctggtc
    36961 aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag
    37021 gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc
    37081 aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac gcaggtggca
    37141 acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa
    37201 aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt
    37261 ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg
    37321 acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg
    37381 ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa
    37441 ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga cagctcccat
    37501 tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag
    37561 cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg
    37621 ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat
    37681 ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag
    37741 acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa
    37801 ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc
    37861 gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg
    37921 cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc
    37981 cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg
    38041 acgtgttgcc agccacattg caaaaatgaa ccccaccaga tacatcaggc cgtaccagtg
    38101 aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct
    38161 actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat
    38221 gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg
    38281 gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag
    38341 cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag
    38401 ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag
    38461 caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc
    38521 aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac gaagatcgat
    38581 accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag
    38641 actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt
    38701 cagatcgttg gtgccaacag agatgaaatc gaccctcttt gccagatgcg gcagcataaa
    38761 taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat
    38821 catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc
    38881 gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag
    38941 catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg
    39001 ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc
    39061 gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata
    39121 ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt
    39181 gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt ccgggcttaa
    39241 accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac
    39301 gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg
    39361 cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt
    39421 cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc
    39481 acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa
    39541 gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa
    39601 acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg
    39661 acccagcgca cgtaaatcac cagcccgctc tttgagatag ttatcgctta gcgcggcaaa
    39721 ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc
    39781 aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc
    39841 gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa
    39901 ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag
    39961 cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc
    40021 aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa
    40081 cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag
    40141 gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc
    40201 aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt
    40261 tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc
    40321 cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc
    40381 gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc
    40441 caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt
    40501 taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt
    40561 gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt
    40621 tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta
    40681 gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca
    40741 gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc cgcttaccag
    40801 ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac
    40861 caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc
    40921 tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc
    40981 tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca
    41041 cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc
    41101 atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca
    41161 ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac
    41221 agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc
    41281 tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat
    41341 aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga
    41401 gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag
    41461 atcaaccaat gccgtgttta tccacagaat gtgccactaa gttaagcact gaaccactaa
    41521 aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga ggtttttcac
    41581 agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag
    41641 atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat
    41701 aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa
    41761 ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct
    41821 cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg
    41881 ggagaactgg cggcacttga cgggctggtt acgccagaga atttaaaacg cgataagggc
    41941 tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa
    42001 gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg
    42061 ctggaaacaa cattcgtttg tgttgcccgg ttaacgggta atagcggggt gacctgggaa
    42121 accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc
    42181 agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag
    42241 gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag
    42301 gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat
    42361 gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc
    42421 ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag
    42481 tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca
    42541 gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt gcgctcggta
    42601 cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg
    42661 tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca
    42721 tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg acaaatccgc
    42781 tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct
    42841 tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag
    42901 ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata
    42961 ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta
    43021 tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg
    43081 attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg
    43141 gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg
    43201 cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc
    43261 tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt
    43321 taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg
    43381 taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact
    43441 ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat
    43501 gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga
    43561 tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta
    43621 acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct
    43681 ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac
    43741 cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt
    43801 gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt
    43861 aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta
    43921 accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc
    43981 gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg
    44041 ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat
    44101 tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc
    44161 ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg
    44221 gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt
    44281 ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat tgtcaccatt
    44341 cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag
    44401 tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa
    44461 tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg gttcacccgc
    44521 tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat
    44581 ggccttgatc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc
    44641 actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg
    44701 gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta tacttatcct
    44761 gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg gcgctgccag
    44821 atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc aatgggcacg
    44881 accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa catggcgctg
    44941 ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt tttaccccgc
    45001 tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc
    45061 aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac caccacgcca
    45121 atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag cttcgctgcc
    45181 gcacctgcgc caaccacgat accaatcgct accatcgcgt tgagataggt cggcgtagcg
    45241 ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg cagcgtgaca
    45301 cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc attgcaccat
    45361 agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca ggactgcccc
    45421 ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc gtaggccagt
    45481 gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc ggctacggaa
    45541 ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt tactaactta
    45601 ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc tgcagcacca
    45661 acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact ggctgcgccc
    45721 agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct atccgccacc
    45781 tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg caggatgggc
    45841 tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg
    45901 ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc cttcgaccac
    45961 aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt ctacccagct
    46021 tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt tcagatagcg
    46081 aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact gttgcaactt
    46141 atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc
    46201 gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca gttgttccac
    46261 catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat
    46321 ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc agccgcgctc
    46381 catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct tctccacccg
    46441 cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct cttcgatacc
    46501 agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg taccgggttt
    46561 cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac cgtagccttc
    46621 aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt gtagtttttc
    46681 tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga aacgcgcgta
    46741 gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc
    46801 cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac ctgtaagcag
    46861 tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa
    46921 gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct
    46981 tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa agaggatcag
    47041 cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca aatgagcgaa
    47101 gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat agacccagcg
    47161 cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa actggcggga
    47221 agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt ttaccccggc
    47281 agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc
    47341 actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac tgtatttttc
    47401 aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt ctggggtaaa
    47461 gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac tcagtaaaga
    47521 ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta tttgatgcag
    47581 catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca tcgccacctg
    47641 cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa ccagaccttt
    47701 caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa ccgttgcacc
    47761 agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc ctgtagtggt
    47821 gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac gtaccagatg
    47881 tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg atttcagcca
    47941 acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac gcacaggtaa
    48001 aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa ttagtacgcg
    48061 ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa
    48121 atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt aaaagcgaat
    48181 ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac
    48241 ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc gaataatctc
    48301 accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc gccagcgaga
    48361 aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt actgttacag
    48421 attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat tcatgtgctc
    48481 aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc aacctgaagc
    48541 caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa aggtttgcaa
    48601 tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga aaacacgcca
    48661 ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt tacccactaa
    48721 ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc agtcgccacc
    48781 gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct ggctgtgcat
    48841 agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct ggcgcagcag
    48901 accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt cggtgcaatg
    48961 gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat tggcaacggt
    49021 taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca tcgctgtgct
    49081 gcgttggtcg tccatgccaa aatgatccca gatgccgatt tagcctcatt aatgaaacaa
    49141 atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg ttgtattgct
    49201 cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca aggtcatacc
    49261 cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg tctgcaaggg
    49321 tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt gacatttggc
    49381 gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg aggaagaaat
    49441 ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg cgttctcaat
    49501 gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga tgtgctggtg
    49561 tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac gatggcgacc
    49621 caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga aattactaat
    49681 gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct ggaggcaagt
    49741 catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta attcttcgat
    49801 ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt ctggtaacag
    49861 cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat catgcagtac
    49921 cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc ccccttcctg
    49981 ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt ccaccgttgg
    50041 cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt aactaccgta
    50101 cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt ggcggctacc
    50161 catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta ccaggaagcg
    50221 acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt gctcacgcgc
    50281 ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct gttgataagg
    50341 aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt cgataacctg
    50401 acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag aaccaatgtg
    50461 catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg cggcgggcag
    50521 atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt tttggctatg
    50581 tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg aaacctggcc
    50641 cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt cactgacgcg
    50701 ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg ggtgcgtttg
    50761 cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg aatccacttt
    50821 cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt tctgtgcaaa
    50881 gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt gcgcatcgta
    50941 gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg cggtgagatc
    51001 ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc ttttttatga
    51061 ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta tgcaaaaatg
    51121 atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac gtcatattga
    51181 aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc tgctacacac
    51241 ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg ggctgaaatt
    51301 gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc tgtttgaaga
    51361 agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa gtctgcgcga
    51421 gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat cttttttacc
    51481 gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata tcgtgcccca
    51541 ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag gactcactga
    51601 gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag atgaagtgtg
    51661 tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg atgattttca
    51721 gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc tggatcagct
    51781 atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg ccgcgtcagt
    51841 ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca ccgcactaga
    51901 ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt tcaccgtcag
    51961 cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta gtgggcattt
    52021 acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt cagctacgac
    52081 agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac aggcaaaaca
    52141 ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac gccctgtgcg
    52201 ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc ttcggtaaat
    52261 tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc atccgcttca
    52321 ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa atcctgttcc
    52381 atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt aattgcacgt
    52441 ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat ggcatccgcc
    52501 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
    52561 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
    52621 tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag cccaccaagc
    52681 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
    52741 cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc gcaacaaggc
    52801 tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat taacgaaaaa
    52861 agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt ctcaacttct
    52921 cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt tccggaatga
    52981 gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag aacgtaccgg
    53041 cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata ttcgacaccc
    53101 agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc tgaatttcag
    53161 agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata cacatcatcg
    53221 tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca aactgcatca
    53281 ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt ttcagagccg
    53341 gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac ataaaggtca
    53401 gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc gccattttga
    53461 agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc atcgcctgca
    53521 acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac agtgcccaac
    53581 cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc gctttttccg
    53641 acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc ttttccgcca
    53701 gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact gctggtaaag
    53761 caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta tcggataaaa
    53821 acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc ttaccgcgaa
    53881 cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg atcccgacag
    53941 caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca aaagcggacc
    54001 ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta cgccccaggc
    54061 ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc atgctactaa
    54121 ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac ggcaacgctc
    54181 cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca gctaccgaaa
    54241 caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca gattccgtat
    54301 tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa atacgtgaac
    54361 aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc tgcgacatgc
    54421 cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat tattattaac
    54481 ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt gatatggatt
    54541 atttcataac catgatatgg attatgatga tctagagata taaaaaaccc tgccatgcgg
    54601 cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat ccacagcaat
    54661 ggtataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg gccccatcag
    54721 gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg cgctacgttg
    54781 ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag ctatcgcatt
    54841 aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc
    54901 gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga gcattgacgc
    54961 gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg ctgcctgaga
    55021 catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt tttcgctgaa
    55081 ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat caatatgacc
    55141 aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc gcagatcggc
    55201 ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt cagtcggttc
    55261 aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg
    55321 gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag aaaatgcact
    55381 taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc tttaacgccc
    55441 acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat aaaggtataa
    55501 gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc ctgctcgtta
    55561 tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca
    55621 tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg acacggcatg
    55681 gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg acaggtttcc
    55741 agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag gttatcgcct
    55801 aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg
    55861 ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc aaagacaact
    55921 tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata gcattgacca
    55981 tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg acgttcaagg
    56041 aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt tttagttatc
    56101 ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt gtactcatcg
    56161 gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc cagcgttttt
    56221 gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa tcagtgaagc
    56281 gtgaaaatag aaaacaatag tgaaacactg tttcgttttc tctataaagg gatgatatgc
    56341 ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat ttaaggatta
    56401 aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag ggagcccaga
    56461 aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc agatatttat
    56521 cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca cgccctggcc
    56581 cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca tttcatgaac
    56641 cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc
    56701 accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg
    56761 tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc agcctacacg
    56821 ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg gatctacacc
    56881 aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc
    56941 ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat tccccgcggt
    57001 cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg cactggcgtc
    57061 agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt ggatcattac
    57121 cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga
    57181 gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag ccagattttc
    57241 ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat gcaccagact
    57301 gtcaaccaac tggctattgc caagctgttc accggtgcgg ctatcagtca gaacatgtgg
    57361 tgcgcgactc atgttttcct ggccacctgc gatgacaata tcagcctcgc cacactgtat
    57421 cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat taatcgtgat
    57481 tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg gattctgccc
    57541 tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg caggaacgcc
    57601 ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg aatgacccgc
    57661 taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt
    57721 catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt acagttatct
    57781 taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca gtagtcagag
    57841 tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt agatagggcg
    57901 ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt ttaatttgta
    57961 gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg tgaagtttcg
    58021 cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag ttaggttcgg
    58081 caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca tattggccac
    58141 ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa gggataacgg
    58201 gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg ctgtacttca
    58261 tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca cttacggaac
    58321 agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt ctgggtgttg
    58381 tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg gtaatgggat
    58441 ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt gtcggtagtt
    58501 ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt caccagatat
    58561 ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt tctacgtttg
    58621 ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa aaaattatga
    58681 aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc tgcctgcttt
    58741 ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc
    58801 tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt gtcgcagtag
    58861 ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt gccacagagg
    58921 tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt aaccacgcac
    58981 tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac ccgaacgcga
    59041 tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc atcatgccta
    59101 cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga aatctgatta
    59161 cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat ccgttgtgga
    59221 tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca tttgttatta
    59281 ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa acacacaaag
    59341 agatctgaac atcttaaata agactattta agatgcataa cttagattcg caagatatct
    59401 tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga tagttatctg
    59461 gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta ataacgagaa
    59521 atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg caatttttcc
    59581 cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa attatgcaat
    59641 tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa agtcttacat
    59701 gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact
    59761 gcgcaaactc gt
//
LOCUS       DAJTRW010000013        58322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRW010000013 DAJTRW010000000
VERSION     DAJTRW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1095)
                     /locus_tag="OO326_000870"
     CDS             complement(<1..1095)
                     /locus_tag="OO326_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5965359.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEG"
     gene            complement(1307..1654)
                     /gene="pspE"
                     /locus_tag="OO326_000871"
     CDS             complement(1307..1654)
                     /gene="pspE"
                     /locus_tag="OO326_000871"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5965360.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1697..1918)
                     /gene="pspD"
                     /locus_tag="OO326_000872"
     CDS             complement(1697..1918)
                     /gene="pspD"
                     /locus_tag="OO326_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5965361.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1927..2286)
                     /gene="pspC"
                     /locus_tag="OO326_000873"
     CDS             complement(1927..2286)
                     /gene="pspC"
                     /locus_tag="OO326_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5965362.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2286..2510)
                     /gene="pspB"
                     /locus_tag="OO326_000874"
     CDS             complement(2286..2510)
                     /gene="pspB"
                     /locus_tag="OO326_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5965363.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2564..3232)
                     /gene="pspA"
                     /locus_tag="OO326_000875"
     CDS             complement(2564..3232)
                     /gene="pspA"
                     /locus_tag="OO326_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5965364.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3399..4376
                     /gene="pspF"
                     /locus_tag="OO326_000876"
     CDS             3399..4376
                     /gene="pspF"
                     /locus_tag="OO326_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5965365.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4496..5761)
                     /gene="puuE"
                     /locus_tag="OO326_000877"
     CDS             complement(4496..5761)
                     /gene="puuE"
                     /locus_tag="OO326_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5965366.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5799..7079)
                     /gene="puuB"
                     /locus_tag="OO326_000878"
     CDS             complement(5799..7079)
                     /gene="puuB"
                     /locus_tag="OO326_000878"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5965367.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7081..8568)
                     /gene="puuC"
                     /locus_tag="OO326_000879"
     CDS             complement(7081..8568)
                     /gene="puuC"
                     /locus_tag="OO326_000879"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5965368.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKHLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8718..9275)
                     /gene="puuR"
                     /locus_tag="OO326_000880"
     CDS             complement(8718..9275)
                     /gene="puuR"
                     /locus_tag="OO326_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5965369.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9302..10066)
                     /gene="puuD"
                     /locus_tag="OO326_000881"
     CDS             complement(9302..10066)
                     /gene="puuD"
                     /locus_tag="OO326_000881"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5965370.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10278..11696
                     /gene="puuA"
                     /locus_tag="OO326_000882"
     CDS             10278..11696
                     /gene="puuA"
                     /locus_tag="OO326_000882"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5965371.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11845..12009
                     /locus_tag="OO326_000883"
     CDS             11845..12009
                     /locus_tag="OO326_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965372.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11999..13384
                     /gene="puuP"
                     /locus_tag="OO326_000884"
     CDS             11999..13384
                     /gene="puuP"
                     /locus_tag="OO326_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5965373.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13518..13763
                     /locus_tag="OO326_000885"
     CDS             13518..13763
                     /locus_tag="OO326_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5965374.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14076..15719
                     /gene="sapA"
                     /locus_tag="OO326_000886"
     CDS             14076..15719
                     /gene="sapA"
                     /locus_tag="OO326_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5965375.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15716..16681
                     /gene="sapB"
                     /locus_tag="OO326_000887"
     CDS             15716..16681
                     /gene="sapB"
                     /locus_tag="OO326_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5965376.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16668..17558
                     /gene="sapC"
                     /locus_tag="OO326_000888"
     CDS             16668..17558
                     /gene="sapC"
                     /locus_tag="OO326_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5965377.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17558..18550
                     /gene="sapD"
                     /locus_tag="OO326_000889"
     CDS             17558..18550
                     /gene="sapD"
                     /locus_tag="OO326_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5965378.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18552..19358
                     /gene="sapF"
                     /locus_tag="OO326_000890"
     CDS             18552..19358
                     /gene="sapF"
                     /locus_tag="OO326_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5965379.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19426..19779
                     /locus_tag="OO326_000891"
     CDS             19426..19779
                     /locus_tag="OO326_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5965380.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20147..20935
                     /gene="fabI"
                     /locus_tag="OO326_000892"
     CDS             20147..20935
                     /gene="fabI"
                     /locus_tag="OO326_000892"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5965381.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21079..22206
                     /locus_tag="OO326_000893"
     CDS             21079..22206
                     /locus_tag="OO326_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5965382.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22274..24208
                     /gene="rnb"
                     /locus_tag="OO326_000894"
     CDS             22274..24208
                     /gene="rnb"
                     /locus_tag="OO326_000894"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5965383.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24286..24389)
                     /locus_tag="OO326_000895"
                     /pseudo
     CDS             complement(24286..24389)
                     /locus_tag="OO326_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24443..26417
                     /gene="pdeR"
                     /locus_tag="OO326_000896"
                     /pseudo
     CDS             24443..26417
                     /gene="pdeR"
                     /locus_tag="OO326_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26565..26738
                     /locus_tag="OO326_000897"
     CDS             26565..26738
                     /locus_tag="OO326_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965384.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26828..27577
                     /locus_tag="OO326_000898"
     CDS             26828..27577
                     /locus_tag="OO326_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5965385.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27846..28064
                     /gene="osmB"
                     /locus_tag="OO326_000899"
     CDS             27846..28064
                     /gene="osmB"
                     /locus_tag="OO326_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5965386.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28190..28516)
                     /gene="yciH"
                     /locus_tag="OO326_000900"
     CDS             complement(28190..28516)
                     /gene="yciH"
                     /locus_tag="OO326_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5965387.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28516..29253)
                     /gene="pyrF"
                     /locus_tag="OO326_000901"
     CDS             complement(28516..29253)
                     /gene="pyrF"
                     /locus_tag="OO326_000901"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5965388.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29446..30615)
                     /gene="lapB"
                     /locus_tag="OO326_000902"
     CDS             complement(29446..30615)
                     /gene="lapB"
                     /locus_tag="OO326_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5965389.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30622..30930)
                     /gene="lapA"
                     /locus_tag="OO326_000903"
     CDS             complement(30622..30930)
                     /gene="lapA"
                     /locus_tag="OO326_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5965390.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31079..31843)
                     /gene="pgpB"
                     /locus_tag="OO326_000904"
     CDS             complement(31079..31843)
                     /gene="pgpB"
                     /locus_tag="OO326_000904"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5965391.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32013..32603
                     /gene="ribA"
                     /locus_tag="OO326_000905"
     CDS             32013..32603
                     /gene="ribA"
                     /locus_tag="OO326_000905"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5965392.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32667..35342)
                     /gene="acnA"
                     /locus_tag="OO326_000906"
     CDS             complement(32667..35342)
                     /gene="acnA"
                     /locus_tag="OO326_000906"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5965393.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35506..35601)
                     /locus_tag="OO326_000907"
     CDS             complement(35506..35601)
                     /locus_tag="OO326_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965394.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35715..35882)
                     /locus_tag="OO326_000908"
     CDS             complement(35715..35882)
                     /locus_tag="OO326_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965395.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35885..36049)
                     /locus_tag="OO326_000909"
     CDS             complement(35885..36049)
                     /locus_tag="OO326_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5965396.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36344..37318)
                     /gene="cysB"
                     /locus_tag="OO326_000910"
     CDS             complement(36344..37318)
                     /gene="cysB"
                     /locus_tag="OO326_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5965397.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37528..40125)
                     /gene="topA"
                     /locus_tag="OO326_000911"
     CDS             complement(37528..40125)
                     /gene="topA"
                     /locus_tag="OO326_000911"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5965398.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40505..40756
                     /locus_tag="OO326_000912"
     CDS             40505..40756
                     /locus_tag="OO326_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5965399.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40792..41841)
                     /gene="sohB"
                     /locus_tag="OO326_000913"
     CDS             complement(40792..41841)
                     /gene="sohB"
                     /locus_tag="OO326_000913"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5965400.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42061..42819
                     /locus_tag="OO326_000914"
     CDS             42061..42819
                     /locus_tag="OO326_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5965401.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42816..43406
                     /gene="cobO"
                     /locus_tag="OO326_000915"
     CDS             42816..43406
                     /gene="cobO"
                     /locus_tag="OO326_000915"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5965402.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43446..44318)
                     /gene="rluB"
                     /locus_tag="OO326_000916"
     CDS             complement(43446..44318)
                     /gene="rluB"
                     /locus_tag="OO326_000916"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5965403.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44419..45039)
                     /locus_tag="OO326_000917"
     CDS             complement(44419..45039)
                     /locus_tag="OO326_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5965404.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45036..45917)
                     /gene="yciV"
                     /locus_tag="OO326_000918"
     CDS             complement(45036..45917)
                     /gene="yciV"
                     /locus_tag="OO326_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5965405.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46055..46099
                     /gene="trpL"
                     /locus_tag="OO326_000919"
     CDS             46055..46099
                     /gene="trpL"
                     /locus_tag="OO326_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5965406.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46076..46169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46191..47753
                     /gene="trpE"
                     /locus_tag="OO326_000920"
     CDS             46191..47753
                     /gene="trpE"
                     /locus_tag="OO326_000920"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5965407.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47753..49348
                     /gene="trpD"
                     /locus_tag="OO326_000921"
     CDS             47753..49348
                     /gene="trpD"
                     /locus_tag="OO326_000921"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5965408.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49349..50710
                     /gene="trpCF"
                     /locus_tag="OO326_000922"
     CDS             49349..50710
                     /gene="trpCF"
                     /locus_tag="OO326_000922"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5965409.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50722..51915
                     /gene="trpB"
                     /locus_tag="OO326_000923"
     CDS             50722..51915
                     /gene="trpB"
                     /locus_tag="OO326_000923"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5965410.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51915..52721
                     /gene="trpA"
                     /locus_tag="OO326_000924"
     CDS             51915..52721
                     /gene="trpA"
                     /locus_tag="OO326_000924"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5965411.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53101..53280
                     /locus_tag="OO326_000925"
     CDS             53101..53280
                     /locus_tag="OO326_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5965412.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53366..53866
                     /locus_tag="OO326_000926"
     CDS             53366..53866
                     /locus_tag="OO326_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5965413.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53912..54418
                     /locus_tag="OO326_000927"
     CDS             53912..54418
                     /locus_tag="OO326_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5965414.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54478..55116)
                     /gene="ompW"
                     /locus_tag="OO326_000928"
     CDS             complement(54478..55116)
                     /gene="ompW"
                     /locus_tag="OO326_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5965415.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55356..55445
                     /locus_tag="OO326_000929"
                     /pseudo
     CDS             55356..55445
                     /locus_tag="OO326_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55473..56216
                     /locus_tag="OO326_000930"
     CDS             55473..56216
                     /locus_tag="OO326_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5965416.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56246..56785
                     /locus_tag="OO326_000931"
     CDS             56246..56785
                     /locus_tag="OO326_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5965417.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56890..57288
                     /gene="yciA"
                     /locus_tag="OO326_000932"
     CDS             56890..57288
                     /gene="yciA"
                     /locus_tag="OO326_000932"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5965418.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57328..58056)
                     /gene="tonB"
                     /locus_tag="OO326_000933"
     CDS             complement(57328..58056)
                     /gene="tonB"
                     /locus_tag="OO326_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5965419.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT        14002 a        15089 c        14576 g        14655 t
ORIGIN      
        1 accttcctgc tgtaatgcct cgaccagcgc taatatttcg ctttctggaa gcaagccacg
       61 cagtgggttc aacccgatgc catcgtgaga ggcgaggaag ttaaaccagg tcgttttact
      121 ggagggcaag ctcagacttt gcgcccactg gcacagcgcc tcgacgttct gtttttgcac
      181 cgcatgtaac accagcggcg gcagcgagaa ctggtacacc atatgcgctt cgtcatcacc
      241 gttgccaaag taagcaatgt tgtctttgtg cggaacattg gtttcggtaa taatcactgt
      301 acctggtgct acgtcatcaa taatcgaccg taacagcttg ataatcagat gtgttttttc
      361 cagatggatg cagcttgttc ccggctcttt ccacataaag ccaacggcat ccaggcggac
      421 atactcagca cctttctcaa ggtaacagag taaaacatcc accatcgcca gcaacacttc
      481 agggctacgg taattcaggt caatttgatc gtcactaaag gtggtccata aatggcgcgt
      541 ggaattatca tccatttgaa acggcgttaa tagcggtaac gcacgcggaa gggtgacggc
      601 actgagatcg gttttcggat caaccgcaat aaagaaatct tcaaaacccg gaagctgttg
      661 taaatagttt ttaaaccatt cactttttgc agacatatgg ttgcagacaa aatcaaacat
      721 taaatgactg cattcaccga gttgctgaat atcctgccac tcccccgctt cactggcgac
      781 ctgatgataa tcaattaccg aaaatccatc atcagatgac catgggtaaa aaggtaataa
      841 atgaacatgt gaaaaaatgc tttgcagcca ttgatggtag aactgattaa atatgggtag
      901 tggttttaaa tcattactgt gaaattgatc ggcataggta attaacacga catcactttc
      961 atcccagtgt tttttacgtc gctgtgtaat taatcgtttc gcactctcaa gacgcgttac
     1021 aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca tgtaatccgt
     1081 aattttctgt ttcatgttgc ctttccgtgg tagcggtccc atgaggcgag agactactcc
     1141 gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa tcatttttta
     1201 ccagggaaaa agcgtatttg tgagctgacg caaataatct atagggatgt aaataaggtg
     1261 tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa cctttgacct
     1321 tcggcattgc gatgtctttc aggccaccgg cgttctcaac gtgggtatat cccatctcgc
     1381 taaggagctc ttttgcctgc ccggactggc gtccggcatt gcaatacact ttcacggtgt
     1441 cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg ggaatattga
     1501 tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg atccagtgtt
     1561 cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct ttttttaaac
     1621 atgtaagcgt ccttctatgg atgaaaattg ccatccatta tacgcctgag atttaacaat
     1681 ttttgcgaaa ctcccctcac cttttataac gctgtgccag tttattagca gcccgactta
     1741 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
     1801 tcgcccagcc cgccacaccc gccgggccat agcgcagtgc ggtaagaagt accagcttgc
     1861 ctgctaattt gaaaccaagc ttcacctttt gcccggcctg ttgccagcga gtattcataa
     1921 ctttcctcac agttgacgga aacggctacg taacgtgaaa gtatcggaag tgacataacg
     1981 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
     2041 ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
     2101 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaaa aaatcgacag
     2161 caccaccagg atacgcacca gttttaccgg tacatcaaaa tagttggcaa tcccggcgca
     2221 gacgccgcgg accatgccct gctgtggaat acgccataac tttttattga gattaatgcc
     2281 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc cagcgcctga
     2341 atacgttcgc gcatccgttt tgcttcatca accagttgcg ctaatcgctg ctgctcactt
     2401 tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca gatcggtaaa
     2461 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aagtactcct
     2521 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga ttgtcttgct
     2581 tcattttggc ttttaattgt gccagttgtt cgctgattgc atcatcggct ttcagttcgg
     2641 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
     2701 tctggtcaat acgacgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
     2761 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatgacgt aacatcaatg
     2821 cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag ctcgccaatc tctttcttca
     2881 tgcgtgccag cgtatcgtcc accagcgtca cttcatgttc cagggactta atcagatcgg
     2941 tcagtttctg tttttcaatt aacgctgcac gtgccagatc ctctctctct ttcagcagcg
     3001 ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca atacggcgag
     3061 tcagctgttt cttttctgcc aacgcacgcg ccgaagtaga acgtacttca accagtgtat
     3121 cttccatctc ctggatcatc agacgcacca gtttctgtgg atcttccgct ttctctaaca
     3181 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
     3241 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
     3301 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
     3361 gaattagcga attacactaa caagtggcga atttcatcat ggccgaatac aaagataatt
     3421 tacttggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccgc
     3481 tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg taaagagttg attgccagcc
     3541 gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
     3601 taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
     3661 cgcaaaaacg tcatcctggg agatttgaac gtgccgacgg cggtacgcta tttcttgatg
     3721 aactcgctac ggcaccgatg atggtgcagg aaaaattatt gcgtgtgatt gagtacggtg
     3781 aactggagcg cgttggcggt agccagccgt tgcaggtgaa tgtgcggttg gtatgcgcga
     3841 cgaatgccga tctcccggcg atggtcaatg aaggtacttt tcgcgctgac ctgctcgacc
     3901 ggctggcttt cgatgtagtg caactgccgc ccctgcgcga gcgcgaaagc gacattatgc
     3961 tgatggcaga acactttgcc attcagatgt gtcgggaaat caagctgcct ctgttcccgg
     4021 ggtttacgga gcgcgccaga gaaacattgc tgaattatcg ttggccggga aatattcgtg
     4081 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
     4141 atgacatcat tattgatccc tttaaacggc gtccacctga agaagccatc gccgtttcag
     4201 aaaatacctc gcttccgacg ctgccgctgg atttacgcga gtttcagatg cagcaggaaa
     4261 aagagttgct gcaactcagt ttgcaacagg ggaaatataa ccagaagcgc gcggctgaat
     4321 tactggggtt aacttatcat cagttccgcg cgttgttgaa aaagcaccag atttagcgaa
     4381 gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt attcggcata cataatcaat
     4441 acgtcgtttg caggcctgat aagcgcagcg catcagacat tattgcgttg ggctattaat
     4501 cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcgcatcc gggatggtca
     4561 gcggatacag gaagcgaatc acgttgccgt atgcgccaca ggtcagcagg agcagcccct
     4621 gcgccagcgc gcgttgctgg attttctgtg caatcgccgc tgacggctcg cccgtttgcg
     4681 gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg taccgcagca atggccggaa
     4741 cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg gcccagttga ttcgcgcgtt
     4801 cgcagagtga ttctttgtcg ataatattga gcaccgcgtg cgcggcagcc accgccagcg
     4861 ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg cgcgtccata atattcgcgt
     4921 taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct tttcgccatc gtcattaagt
     4981 ccggcttatc ggcgtaatga tccatggcaa acagtttacc ggtacgcgca aagccgcttt
     5041 gcacttcatc ggcaatcatc acaataccgt gctcgtcgca caggcggcga atggcggcaa
     5101 ccagctcttt tggcgcaacg ttgaaaccgc actcgccctg caccggttcg aaaataatgg
     5161 ccgcgacctg cttcgcttcg atgtctgatt taaacaagcg ttcgatggcg tcgagggagt
     5221 cctgtgttga aatgccgtgt aaatctgacg gataaggtac gtgatacacc gaaccaggga
     5281 acgggccgaa gccgattttg tacggcgcaa cttttccggt cagcgccatg gtcatatatg
     5341 tacgaccgtg gaagccaccg ctaaacgcaa tcacgccagg tcgtccggta tgggcgcgag
     5401 caattttcac cgcgttttcc accgcttccg cgccggtagt gaagaatgcg gttttagcct
     5461 gcccgctcac tggggcaagg gtgttaattt tctccgccag cgtgacgtag ctttcgtacg
     5521 gcacaatctg atacgccgtg tgggtaaact gttgcaattg ctgctccacc gccgcgacca
     5581 gatcagggtg gcgatgcccg gtattcagca ccgcaatgcc tgcggcgaaa tcgatgtatt
     5641 cgttgccctc tacgtccttc agcgtagcgt tttctgccga ctgggcgaag aagttacaca
     5701 tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg atggaattca ttgttgctca
     5761 tgtttcacac cttttgactc ttcactgtta atcggaaatt aaacgcccag acgatcgcgc
     5821 aggctgtaat aagccgcgcc catcgcggta aatggcacgc gcagcgtgcg cccgccgggg
     5881 aacgggtaat gcggcagatt ggcgaaggca tcgaaacgct cggcgtcacc gcgcagcagt
     5941 tcagcaatca aacgcccggc aagatgggta caggtgacgc cgtggccgct gtaaccctgc
     6001 atgtaataga tgttggtatc aaggcggccg aactgcggca tgtgcgacaa ggtcagcagg
     6061 aagttgcccg tccagcggta atcaattttc acgcccttca actgagggaa ggtttttagt
     6121 agtttcggta ccacaagacg ctcaacgtca tccgggttgc gtgcaccata cactacaccc
     6181 ccgccgtaca gcaggcggtt gtcggcggta agacggtagt aatcaagcag atagttacag
     6241 tcttccacac agtagttttt cgggatcagc gaacgggcta aatcttccga cagccgttcg
     6301 gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg ctagttccgg ctctacttta
     6361 tcgcccagat acgcattccc ggcgacaatc acatacttcg ccgtcacctg accattggca
     6421 gtccgaacca cggctggcgt ggtgtgctgg atctgcgtca cggcggaaag ctcatacacg
     6481 cgcccgccgt tcaggcggat ggcatcggct tcaccaatgg cgaggtttag cgggtgaata
     6541 tgcccaccgc tgtgatccag cagcgcacca gtgtagcgat cgctggcgac ctcgcggcga
     6601 atagcgttga cgtccagcag ctccagctgt ttattgccgt agcgtaccca gttctctttc
     6661 tgctcttcga gtgtggcgag ttgtttatca ttcatcgcca caaacagacc gccggggcgg
     6721 tagtcgcagt caatctgata acgtttgata cgttcgcgga tgatctcgcc accctcgaac
     6781 atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc ttttttcgat cacgtcgatg
     6841 tcgcggctgt aggagttcac aagctgcccg ccattgcgcc cgcttgcgcc aaagccgatg
     6901 cgtgaggctt cgagaaccac tacgtcaaag cccgcttccg ccagatgcag cgcggaggag
     6961 agcccggtat agccgccgcc aaccacgcaa acgtcgcagg tgattgactc attcagtgtg
     7021 tcgaatggtg catatttatt cgcactggcg gcgtagtaac tgctggtatg ttcggtcatt
     7081 tcaggcctcc aggcttatcc agatggtttt cagttcagtg aatttttcaa gggcatgcag
     7141 ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
     7201 gtcgttgtag ttattgacga agacggaacc ggctttcagg cgtcggctca tgcggtgcgc
     7261 gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
     7321 ctgcagcgcc tgatcttctg atgtgaaacg ggtgaccacc agcaccggac cgaaaatctc
     7381 ttcgcggctt aaggacgcat tcgggtccac atccacgaag atggtcgggc cgatggccgc
     7441 tgccagcccg gcgttgcgac catccagcag cagttgcccc ttgccttcgc cttcacgaat
     7501 aaaggtgtgt accgagtcag cgtgagcgct gtcgattaag gtgcccatgg tggttgcggg
     7561 atcaagtgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
     7621 atcggcgatg ctctcttcca gtaacaaacg cgttccggcg atgcgcacct ggccctggtt
     7681 gtagaagatg cctgcggcgg tggcgcttgc cgctttttgc agatccggac aatcggcgaa
     7741 aacgatgttg gcgcttttgc cgcccgcttc cagccagaca cgtttcatgt tgctatcacc
     7801 cgcgtctttc agcaggtgtt tacccgtacg cgtggagccg gtaaaggcga tggcatcgat
     7861 atcgttatga cgcgaaagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa
     7921 cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg cactgagcgg
     7981 tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt
     8041 ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
     8101 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
     8161 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
     8221 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
     8281 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
     8341 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac
     8401 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg accggatcaa cggtttcaaa
     8461 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
     8521 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
     8581 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggctgc
     8641 atgcccgttt atttctggcg acattcttta tggattgccg ggcgcattgc gcttacccgg
     8701 cttacagaac aaaaagatta aaacgtggtg ggcgtatggg cgctgataat tcggcaaata
     8761 cctgccgacg tattactgaa actgtgcggg atgccggtat taatggcata actttgcccc
     8821 gcgacgaggt ggtaatcctg accattaatc gtcagaacaa tttcaccttc cagtactgtg
     8881 cctatttcct caccctgatg cttaattctt tccccagtgg ttgtgcccgg ctggtacgtt
     8941 tcaaagatca tcgccagcgt gcgattcggg ttaccgttat gaaccagctt cattgacaca
     9001 ccctgactgc ccatctcaat taagtcgtcc tgattaatga cgacctgcgg ctcatcaggt
     9061 ttttccggct cggaaaagaa ttccgagagt gacagaccat acaccttcag cagcttttgc
     9121 agcgtactga tggcagggct gactttatct tgttctatcg tactgatagc actgtgagtc
     9181 agcccggaga gttcggcggc acgacgttgt gaaagcccct gctgctggcg gatttccgac
     9241 aagcgttttc ctggcgccag tccctcatca ctcatatttg catttcctta aactgtagtg
     9301 gtcagagtcg ctgtttttca gcgatatggt gctgccaagc ggtgataaag ccctcgaaca
     9361 atatacgcga aagcgcgtac tcgctactgt tccattccgg gtgccactgt acgcccagcg
     9421 caaaaggatg attgatgacg ctaaccgcct caactaagcc atccggcgag cgagcttcaa
     9481 cacgcaaccg tgggctaacg accttcgccc cttgtccatg tagagagttt acccaaaagt
     9541 tgctacattc aggtaacaac gcagacagta atcccccctc ttcaacctga acttcatgcg
     9601 acggtgcata ttgctgttcc accggcagtt cgggatcttc ccgatgttcc agcaattcag
     9661 gctgttcgca cagcttgcga tgcaacgaac caccggttgc caccaccagt tcttgtaaac
     9721 cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc ggcattaatt atcgccatgc
     9781 tcagaagatc acgcccggga tcggcgtcag gctcatcgcc gttttcacca tataggtgcg
     9841 gctgcacatt gctgggacta ctaggaagat aaatgccatc gagtttcggc aaaagttgtt
     9901 caagtaacga cggttccgcc agcgcatgtg gtagcgcaat tggcaagccg cctgcgtgga
     9961 tgatggcatt caggtacttt tcttgcagag tctgggtcgc atgaccctta agcctgttcc
    10021 tgcacattac gacaccgata accggattgt tcattatatt ttccatgctc aatctcacaa
    10081 aatggactaa attatcgcca ttactgcgct ttaaatgacc tttatgttca atattttttc
    10141 aatctggcag tggattttca tttttgcaaa ctcaatttaa catttgacaa acatttggtt
    10201 tgcatacaga ttcgaatgat ggtcattata ttttacgctt tgataacgag cggaaaacaa
    10261 accaaaggcg aagaatcatg gaaaccaata tcgttgaagt agagaacttt gttcagcagt
    10321 cagaagagag gcggggtagc gcctttacgc aggaagtgaa acgctacctg gagcgctacc
    10381 cgaatacgca atatgttgat gttttactta ctgatttgaa tggttgcttc cgtggaaaac
    10441 gtatccctgt ttcaagcctg aagaagctcg agaaagggtg ttatttcccg gcctcggtat
    10501 ttgcaatgga tattctgggc aacgtagtag aagaagcagg tctgggtcag gaaatgggcg
    10561 agccggatcg tacctgtgtt cctgttctcg gttccttaac tccttctgcc gccgatccag
    10621 agtttatcgg tcagatgctc ctgaccatgg tcgatgaaga tggcgctccc tttgacgttg
    10681 agccgcggaa cgttcttaac cgcctgtggc agcagctgcg ccagcgcgga ttgttcccgg
    10741 ttgtagcggt agagctggag ttctatttac tggatcgcca gcgcgacgct gaaggggatc
    10801 tgcaaccgcc ctgcgcgcct ggcaccgatg accgcaatac acaaagccag gtttactccg
    10861 ttgataacct caaccacttc gccgacgtgc tcaatgatat tgatgaactg gcgcagttgc
    10921 agctgattcc ggcagatggc gcggtcgctg aggcctcgcc gggtcagttt gaaatcaacc
    10981 tttaccatac tgataacgtg ctggaagcct gcgatgatgc gctggcacta aaacgcctag
    11041 tgcgtctgat ggcagaaaag cataagatgc acgccacttt tatggcgaag ccgtatgaag
    11101 agcacgcggg cagcgggatg catatccata tcagtatgca aaataatcgt ggcgagaatg
    11161 tgctttctga cacagaaggc gaagattcgc cgctgctgaa aaagatgctc gccgggatga
    11221 ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa cgtgaactcg tatcgccgct
    11281 tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg ccataacaac cgcaccgtcg
    11341 ccctgcgtat tccgtgcggt gaccgtcata atcaccgcgt ggaatatcac gtggcgggtg
    11401 ccgatgccaa cccatatctg gtgatggcag cgatttttgc cggtattttg catggccttg
    11461 ataacgagct gccgttgcag gaagaagtcg aaggcaacgg gctggaacag gaaggcttac
    11521 ccttcccgat tcgccagagc gatgccctgg gtgagtttat cgagaatgat cacctgcgcc
    11581 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    11641 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    11701 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    11761 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    11821 agactgtgac gaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    11881 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    11941 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    12001 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    12061 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    12121 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    12181 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc gccagtttcc
    12241 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    12301 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    12361 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    12421 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    12481 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    12541 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    12601 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    12661 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    12721 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    12781 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    12841 gctgtacgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    12901 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    12961 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    13021 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    13081 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    13141 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    13201 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    13261 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    13321 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    13381 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    13441 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    13501 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    13561 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    13621 acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa gaagcccaac
    13681 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    13741 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    13801 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    13861 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    13921 gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa cgaccaacca
    13981 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    14041 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    14101 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    14161 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    14221 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    14281 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    14341 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    14401 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    14461 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    14521 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    14581 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    14641 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    14701 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    14761 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    14821 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    14881 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    14941 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    15001 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    15061 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    15121 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    15181 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    15241 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    15301 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    15361 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    15421 gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcaatc
    15481 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca gcgcgtattg
    15541 aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg ccgctggcgt
    15601 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    15661 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    15721 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    15781 tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt cactgtggaa
    15841 tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt ccagcattaa
    15901 cggtcaacca attgccgaac agttaaaaga gctcttcccg gcgacgatag aactgtgcat
    15961 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    16021 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    16081 ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag gttggttgcc
    16141 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    16201 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    16261 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    16321 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    16381 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    16441 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    16501 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    16561 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    16621 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    16681 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    16741 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta ctgtgtattt
    16801 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    16861 cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    16921 gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    16981 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    17041 gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc ccttcgctgc
    17101 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    17161 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    17221 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    17281 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    17341 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    17401 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    17461 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    17521 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    17581 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    17641 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    17701 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    17761 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    17821 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    17881 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    17941 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    18001 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    18061 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
    18121 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    18181 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    18241 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    18301 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    18361 gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc tggaacagtt
    18421 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    18481 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    18541 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    18601 accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    18661 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacactgg
    18721 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
    18781 cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt tttcaggatc
    18841 cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt ccactgcgcc
    18901 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    18961 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    19021 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    19081 ccctcgcttc actggatatg tcaatgcgtt cgcagttgat taatctgatg ctggagttac
    19141 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    19201 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
    19261 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    19321 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    19381 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    19441 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    19501 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    19561 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    19621 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagggctgg
    19681 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt gctggagatt
    19741 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    19801 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    19861 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    19921 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    19981 ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggtaacta tagctactca
    20041 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    20101 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    20161 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
    20221 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    20281 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    20341 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    20401 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    20461 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    20521 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    20581 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    20641 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    20701 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    20761 aatgctggct cattgcgaag ccgttacccc gattcgccgt acagttacta ttgaagatgt
    20821 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt
    20881 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
    20941 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    21001 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    21061 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    21121 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    21181 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc ccccttcctt
    21241 tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca gcttaccgtg
    21301 acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
    21361 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccgga
    21421 cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
    21481 ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    21541 cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    21601 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
    21661 gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    21721 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
    21781 caactggccg aaacactctc tctccaccct atttcacttt cccttctcga aaagttgtta
    21841 aacagcacac gggtcaatgc acagccggat aatcagcttg cggcgttgtt atgcgcgcgt
    21901 ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga atataaaaaa
    21961 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga ccgtcattct
    22021 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    22081 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    22141 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    22201 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    22261 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    22321 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    22381 ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    22441 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    22501 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    22561 tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc gcgcagcccg
    22621 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    22681 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggtgacga
    22741 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    22801 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga cctcgctgga
    22861 ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt tcgctaaggc
    22921 gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    22981 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    23041 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
    23101 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    23161 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
    23221 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgccgaaca
    23281 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    23341 ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    23401 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    23461 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    23521 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    23581 gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact
    23641 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    23701 aattagctct gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    23761 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    23821 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg aacgtcgccg
    23881 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
    23941 taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
    24001 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    24061 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    24121 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
    24181 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    24241 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    24301 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa aacaatgcgt
    24361 ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc
    24421 tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt
    24481 tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgctt tgcgcttttc
    24541 taccaccaaa accacagaac ctgatcgtac attgcagtta tctgccgaac aggctgctcg
    24601 catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgaaag
    24661 cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc
    24721 gtctgcatgg catgacacac cggcggttgc tcgtgattta tcacacgggc tttcctttgc
    24781 tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat
    24841 ccaacgcttc aatcggttat gtgaagatta cacagggttg aaagaacacg acgtcattgg
    24901 gtaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa
    24961 ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacacgtaa
    25021 aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga
    25081 gattttttta atctgttccg gtaccgacat taccgaagag cgccgcactc aggagcgact
    25141 gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga
    25201 gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggttg tgtatcttga
    25261 tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttgtttggtg accagttatt
    25321 acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc
    25381 aggtggcgat gagtttctgg tactggcatc caatacctca caaagcgcgc tggaagcaat
    25441 ggcatcacga attttgaccc gcttacgcct cccctttcgt attggtttaa ttgaagttta
    25501 taccagctgt tcagtaggta tttcgctctc tcccgaacat ggttcggaca gcgcgactat
    25561 tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag gacaattttg
    25621 cgtttttact ccagaaatga atcagcgggt atttgaatat ctctggctgg ataccaactt
    25681 gcgtaaagca ctggaaaacg atcagttggt tatcgaaaat cacctagcgt gtcgaagtgc
    25741 gcagtctgga agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg
    25801 acttcatttc ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc
    25861 tcgatgtcgt acgccaggtg gcaaagtggc gggataaagg cataaacctg cgagtggcgg
    25921 taaatatttc tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc
    25981 tccaggaact caattttgaa tactgcccta tagatgttga actgacagag agttgtctga
    26041 ttgagaatga tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag
    26101 tgcatctgga tgattttggt accggctact cttcactttc gcaactggcg cgctttccga
    26161 tcgatgccat caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc
    26221 agtcactggt ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg
    26281 aaggtgtaga gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc
    26341 aaggattttt gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct
    26401 atctgaagcg cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat
    26461 atcgtattcc tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt
    26521 tttgcattat tcattttccc ccttttgcta taaggatgtc cttgatgtct gaatttgatg
    26581 cccagcgcgt tgccgaacgt attgatattg tgttagatat ccttgtggca ggcgattacc
    26641 actctgcgat ccataatctc gagatactta aagcagaact cttgcgccag gttgctgaat
    26701 ctacgccaga catcccgaag acaccgtggg agatttaaca atcgtgcctt caggttttcc
    26761 tatttgagga tgcccggcgc gtcagtattt ttttatttag tattataacg ttataagaat
    26821 tacagcgatg aattcccgac aacaaactat tctacagatg gtcattgacc agggtcaggt
    26881 tagcgtaacc gatctggcaa aagccactgg agtttctgaa gttaccattc gccaggatct
    26941 caacaccctc gaaaaactga gttacctccg ccgtgcacat ggctttgcag tttcgcttga
    27001 tagtgatgac gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg aactcgccga
    27061 gtttgccgcg tcactggttc aaccgggcga aaccatcttt atcgaaaatg gcagcagcaa
    27121 tgccctgctc gcccggactc tgggggagca gaagaaaaat gtcactatca tcacggtcag
    27181 cagctacatc gcgcatttgc tgaaagacgc gccttgtgaa gtcattttgc tcggtggcgt
    27241 gtaccagaaa aaaagcgaaa gtatggttgg ccctttgaca cgccagtgca tccagcaggt
    27301 acatttcagc aaagcattta ttggtattga tggctggcaa cctgaaactg gattcaccgg
    27361 tcgcgatatg atgcgcaccg atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat
    27421 agtcctgact gacagctcga aatttggtgc tgtacattca tactccatcg gtcccgttga
    27481 gcgattcaat cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga tgcatcttga
    27541 gcacagtaaa ctcaccgttc acgtcgttga catttaacca tttcccttct ctacggatga
    27601 tttgcagttt ggcaaatcat ccgctctaag atgattcctg gttgataatt aagactattt
    27661 acctgttatt aacactctca agatataaaa ttattatcag cgatataaca ggaagtaatt
    27721 atcacctgcg tgatataacc ctgcgcgcga gcagatttca cggaataatt tcaccaggct
    27781 tattcttagc tattatagtt atagagagct tacttccgtg aatcataaat tcaggagaga
    27841 gtattatgtt tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt actttggcaa
    27901 tgtctctgag tgcctgttct aactggtcta aacgggaccg caacaccgca atcggcgcgg
    27961 gtgcaggggc attaggcggt gcagtactga ccgatggcag tacgttgggt acattaggtg
    28021 gtgcagctgt cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg
    28081 attatcgact cgcaattata atacgcttga ttctgttcag tacgaaataa ctgaataata
    28141 cacacagtaa aatattcgaa agccacggat atatccgtgg ctttttatgt taaccgcctg
    28201 cgagttttac cttcatccct ttcgcttcaa gtagtgattt taataaatca cgcttatcgc
    28261 cctggatttc aataactcca tctttaactg ctccgccgca gccgcatttt ttcttcagtt
    28321 ccgctgcaag ttttgtcagt tcggcatcat cgagatcgat accggtaatc aggcaaacgc
    28381 ccttaccttt acgtccactg gtctgacgct gaatacgcac cgctccatcg cctttagggc
    28441 gtactggggc cgctttgggc tcatcaatac gcccggtttc cgttgagtag accagacggc
    28501 tgttggaatc actcatcatg cactccgctg taaagaggcg ttgatcgctt tcagtgtctg
    28561 cgctggatct accgattgcg ttaccgggcg accaatcacc atataatcaa caccagccgc
    28621 caacgcctgt tctggcgtca taatgcggcg ctggtcacca gcttcactcc cctgcggacg
    28681 aatgcccggt gtaaccagtt tgaactcctg accgaatacc tgtttaaagc gcacagcttc
    28741 ctgagcagaa cacaccacac catcaaggtc acatttttgc gtcagtgctg ccagacgttc
    28801 tgcataatct gcaggtgaca gtgtcacgcc aagatcggcc aggtcgctgg cttccatgct
    28861 ggtcaacact gtcacagcaa tcaaaagcgg tgcatctttg ccaaacggaa ccagtgcctc
    28921 acgcgctgcg gtcatcatac gcgccccacc ggaggcatga acattcacca tccacacgcc
    28981 taagtcagct gcagcagcga cagcgtgcgc tgcagtgttg gggatatcgt ggaatttcag
    29041 gtcaagaaag atatcaaaac cacgctgttg aagttcgcgc acaaactgtg gcccaaacaa
    29101 tgtaaacatc tctttaccga ccttcagacg acaatcgcgt gggtcgatct tgtcgacaaa
    29161 ggacagcgcg tcatcacaat tatgataatc aagggcaaca accacaggag aattcgtaac
    29221 ggcgcgggaa gaagatgaag cagttaacgt catgaccaga ccttcttgat gatgggcacc
    29281 gggagtggcg cgaaacaggt aaacggcgag cattctaccc gtgaaaataa aaaattaaca
    29341 gttgcgattt ttcccctagc aggctttttc tgctccgtcg ttgccggagc acaatgggac
    29401 atcaataact agtatgttgt aactaaagtc ggattttttt aaaaattaca ggccatcaag
    29461 accgcgaatc ggtttaatag ttgaccaggc acgacaagac ggacaatgcc agtagagggt
    29521 gtatgcggta aaaccacatt tctggcagct ataacgaggc ttactacgca ccttctcgcc
    29581 aaccatgtca cgcagcacca tcagactctc tttggcgcgc ccttcttccg cttcatttaa
    29641 gtggtaatcc attagcttat ggaatacacg catggtcgga tgacgctgaa gctggcgtgt
    29701 aatatagacc tgtgcagcct cactaccgtc gcgcgcttcg atgatatcag caagcatcaa
    29761 ttcagcatcg gcaccggtgt tctcttccac cgcgcgttgc aggaattctg cccattcggc
    29821 agttttaccc aactgctgat agcaggtttg caacatttcc agcgtttcgc tgaccagttc
    29881 tctgtcctgg gatatgacgc gttgcaaact ttcgacggct ttggcgtatt ctcctttcgc
    29941 cataaacacg cgtcccatca tgatggatac gcgggcgctg tttttatctg ccgccgcccc
    30001 tttttttagc aaggtcatgg cacgatcgag atcgtcgctg gccatatgct gcagggctaa
    30061 ctcacagtag aaatgggcaa tttcgacgcg ctgtttatct ttacccagct tcaccaggcg
    30121 ttcggcaaca tcaattgctt tctgccactc actggtagcc tggtagattt gtagcaactg
    30181 ttgcagcgcg ccaatgcgga agtcagtttc atcggtcagc tgattgaaca tgtcttccgc
    30241 gcggtcgtat aacccggcgg ccatgtaatc acgccccagt tgttgaatcg ccaacagacg
    30301 ctgttcatag gtcagcgagg cgctttccat tagggtctga tggatgcgaa tagcgcgatc
    30361 aacttcgcca cgcgaacgga acaggtttcc gagcgtaagg tgggcttcaa cggtgcctgt
    30421 atcctcttta agcatatcga gaaacaggtc taccgcttta tcctgttgat tactaagcag
    30481 gaagttaacc cccgctacgt aatcacgcga caagcggttg gcttcatctt gcttgttttg
    30541 ttgcgcactt ctgcggccca tataccagcc ataggcagcg gctacaggca aaagcagaaa
    30601 caacaactcc agcatagaaa gttattcctt cgccgctgac gagtgcggca ctacagccac
    30661 gtcagtcgcg ggtgaaagct ggttttccag tcgctttatt ttacgttcag cgcgcgccag
    30721 ggaaacacga actcgcagcc agaacaggcc acaaatcaac caaccgatag caaaccccgc
    30781 agcaaacaat accgccagca atgtggaaat acggtactcc ccttgcgcta acagataatt
    30841 aaacgtcacc tgttgatcgt tctgcgcacc caacgtgacc gaaatcacga agatcgctaa
    30901 caccagtaag aaaatgagta aatatttcac attacttccc gttatgtggt tagagcgaat
    30961 aaattatggt caacttaccg caatagccct ttaaagtacc attttcgcgc cgtctgcgaa
    31021 atggcatcca cgcgcattgt tgatgattta aggcctaaat tcggaaaatc aaccagcgtt
    31081 aactttcttg ttctcgttgt gcaatttcgc gattttcttc cgcaggtggt gttaatggcc
    31141 cacaaattcg ttgcgcaagc cacgttgcca ccgccaccag cgcccacgaa atcaacgtag
    31201 ctaccaccag atcgcgtggc caatgcatcc cgagcagcag gcggcttccc atgactcccg
    31261 ttgcccagac caacaagata gcaatggtta acgttcgccg acgcggccac agcaaaccaa
    31321 cggccagcag tgcccaactg gcagcaaaca tcgtgtgacc ggaaggaaag gcaaaccccg
    31381 tctctttctg ccagtgtgaa cgcaaatatt gtggaatatt tttctcttca gccaactgtt
    31441 cttttactag atttccgcgt tctgctcgct ttaaagtgta gaactcatca accggaatat
    31501 gatgtgtttt ttccaaccag ataacaaaag gtcgtggttc ctgtactttg tctttgatcc
    31561 aggatttaac gccttgtcca acaaggattg ccgccgccag aatggcaaat aatacaaagg
    31621 cagccttaat gcgaaaacgc agacaccaga gaaaccagcc gaacaaaatc aaatgtgtaa
    31681 tgacgcccca gggctgggtg acagtttcag taacccaaaa agccgctttt agtagccaac
    31741 tttgttctcc aggttgccaa cgccagccag aaatccatac ggctactggc atgacaagca
    31801 atagtgcagc tcccactgcg gtacgtcggg caatcgaacg catggcctct cctttttgat
    31861 aagtcccaca atcataactg aaaatgccag ttccaagaaa aattgacaga tttgtgccat
    31921 tccgtgaacg atcgacgcgt cgtgattagg tgaacccctt ctcgttatgg caaaataagc
    31981 caatacagaa ccagcattat ctggagaatt tcatgcagct taaacgtgtg gcagaagcca
    32041 aactgccaac cccatggggc gatttcctga tggtgggatt tgaagaactg gcaaccggac
    32101 acgatcatgt tgcgctagtc tatggcgata tttccgggca taccccggta cttgcgcgcg
    32161 tccattccga atgtctgacc ggtgacgccc tgttcagctt gcgctgcgat tgtggcttcc
    32221 agctcgaagc ggcattgacg caaattgccg aggaaggccg tggtattttg ctgtatcacc
    32281 gtcaggaagg tcgtaacatt ggtctgctga ataaaatccg cgcttacgca ctgcaggatc
    32341 aaggttacga taccgtagag gctaaccacc agttaggctt cgccgctgat gagcgcgact
    32401 tcactctttg cgctgatatg ttcaaactcc ttggcgtcaa tgaagtccgc ttgttaacca
    32461 ataacccgaa aaaagtcgaa attctgaccg aagcagggat taatattgtt gaacgcgtac
    32521 cattgattgt aggtcgtaac cccaataacg aacattatct cgataccaaa gccgagaaaa
    32581 tgggccattt gctgaacaaa taaccctctt gcatcgtgta attcatttgc ttgccggaag
    32641 caaaataacc ggcaagcaaa tacttgttac ttcaacatat tacgaatgac ataatgcaaa
    32701 atgccgtcgt tctggtagta ggtcaactcc gtcgcggtgt cgatacgaca acggcagggt
    32761 acgacttcct ggctaccatc cgcgcgcgta agcgtcaccg gaaccgtcgc gccaggttgt
    32821 agattttgca gatcgccaat atcaatcttc tcttccccgg ttagccctaa cgttttacgc
    32881 gtcacgcctt gcggaaattc cagcggcagg atgcccatgc caattaaatt cgaacggtga
    32941 attcgttcaa acgattcggc aatcaccaca cgaattccaa gcagacgagg tccttttgcc
    33001 gcccagtcac ggcttgagcc tgatccatac tctttcccgg caatcaccgc cagcggcgtt
    33061 tgctcctgct tatagcgcat cgcagcatca taaatagaga ctacgtcgct gtcaggtaaa
    33121 tgccgcgtca tccccccttc aacgccaggc accatttcat tacggatgcg aatattggcg
    33181 aaggtgccgc gcatcatcac ttcatggtta ccacgccgcg aaccgtagga gttaaagtct
    33241 tttcgctcaa caccccgacc ttgtagatat cgacccgctg ggctgtcggg cttaatactg
    33301 cccgccggag agatatggtc agtggtgact gaatccccca gcattgcgag gatccgcgca
    33361 ccgtgaatat cttccactgg tgctggtgtt gcctgcattt catcaaagaa aggcgataag
    33421 cgaatatagg ttgagtcctc ctgccaaccg taggtatcgg atcgtgtgac gttaatttcc
    33481 ttccactctg ctgtgccttc aaaaacttct gcgtactctt tgcggaacat ttctgtggag
    33541 acttgttcta ccgcacgggc aatttcttgt gccgatggcc agatatcttt cagataaacc
    33601 ggctcgcctt tgcgatcatg gccgataggc tcagaagcca ggttgatatt catatttccc
    33661 gccagcgcat aggcaaccac cagcggtggc gaggccagcc agttagtttt aaccagcgga
    33721 tggatacggc cttcaaagtt acggttgccg gacagcaccg caccgacggt taaatcgcct
    33781 tttttgattg ccgtttcgat aggatcgggc agcggcccag agttaccaat acaggtggta
    33841 caaccgtatc ccacaaggtt aaaccccagt tcgtcgagat acggtgtcag tttcgctttt
    33901 gccagataat cagaaacgac tttcgaaccc ggtgccaacg acgctttgac ccacggttgc
    33961 cgcttgaggc caagagttac ggcttttttc gccagcaagc ctgcggccat cagcacactt
    34021 gggttagagg tgttggtgca cgaggttatc gcagcaatga ccacagcgcc atcaggtaac
    34081 tgatactgat gtccgttcat aacataatcg actggcaggc gatctttatg cgtggcattc
    34141 actttcagtt cgttactggc ggcaaatgct tttggtacat cgggcagtgc aacgcgatcc
    34201 tgtgggcgtt ttggccctgc caggctcgct tcaacgtcat tcatatccag ttctaacata
    34261 ctggtaaaaa tcggttcatc gcccgggtta cgccacatgc cctgcgcttt ggcatatttt
    34321 tcgaccaact cgacctgatc ttcgctgcgc ccgcttaaac gcatgtaatc gagggttact
    34381 gcatcgattg ggaagaagcc acaggtggca ccatattctg gcgacatatt ggcaatggtg
    34441 gcgcgatccg ccaacggtag tgaatccaga ccatcaccat aaaattcgac gaatttcccc
    34501 accacgccat gtttgcgcag catttgggta acagtgagaa ccaggtctgt ggcggtaata
    34561 ccttcacgta attttccggt aagtttaaag cccactacgt ccgggataag catggaaacc
    34621 ggctggccta acatggcggc ttctgcttcg atcccaccca cgccccaccc cagcacgcca
    34681 aggccgttga tcatagtggt gtgcgagtca gtaccaacga gtgtatccgg ataagcaatc
    34741 cattcaccgt cctgcaattc gctccacact gctttgccga gatattcgag gttaacctga
    34801 tggcaaatgc ctgtgcctgg cggcacgacg ctaaaccgac tgaacgcttg ctttccccat
    34861 ttcaggaaca cataacgttc gtggttgcgc tccatttcca ggcgtacgtt ttcttcaaat
    34921 gcctcatcat caccaaaacg atcgacggtc accgagtggt caatgaccag gtcgaccggt
    34981 gagagcgggt taacctttgc agtatcgcca ccgaggcgtt taaccgcttc gcgcattgcc
    35041 gccagatcaa caacggcagg tacgccggta aagtcctgca tcagcaccct tgccgggcgg
    35101 taggcaattt cacggtcagc atgggcattt ttcagccatc ccgccagtgc gtggatatcc
    35161 tcttcggtaa ctgagttacc atcctgccag cgcagcaggt tttcgagcaa aactttgagt
    35221 gacttgggta gacgggtgat atcgcccagt gatttagcag caagcggcag gctgtagtag
    35281 tggtaagttt tatctttggc ctgcaacgtg tccttactgg cttctcgtag ggttgacgac
    35341 atagctcctc cttaatgaca gggttgcgaa gccctgattc tcttcagggt gaatattaaa
    35401 gataacacaa acatcgcgta acgatttgat aacaacccaa attgataaaa gaggagaaac
    35461 cttgtggcaa agcaaatgac aaccccgccg cagcggggtc aaggatcagt gagtaatgat
    35521 ccagaggagt tcggcccaga agagcatcga cagagaaaac gcgcccatcc aggaccaata
    35581 tttcatattt gtgttgatca taggctacac ccttaacggt tattattttg tgttgagata
    35641 actcaagctg ttattgctgc ggtgtattaa aagcccttta attcattaaa aatgggtaat
    35701 aagaatgatg ataattattg tttcggctga tcatcttcag caaacagttc tataaaggct
    35761 tgctgctgcg atgttagttg ccagtcgtct ggaataacga cggaacattc ccgtttcgtt
    35821 tctggttcat tgtcactatg ctggcataat ggtgacgact ccagttgctc ctgaccgacc
    35881 atagttatcc ccacactttc caaatcagca gtgcgacaac cacccagaaa agtgcagaac
    35941 caagaaaaac cgccagccag gctttacgtt ttaattcagg atcacggcgg ggttcctgat
    36001 ttccagaagg cattgctaac ctcatataat cgccatcact tatcagcaag acgttgaatg
    36061 attggtgtaa ttaatcatac gatgagacaa atcctaaaga aactttagtc aaaaatccag
    36121 cgaatttacg aatcgtctcc agtgaataat tcaatctttt gacctgaaat agatatttga
    36181 aaactggaat ttgcgaggcg ggtaattaga cactacttta caggtaattt accctataaa
    36241 tatgagggat aaaaatattg tgcacggtat agaaataagt aacaaaagag aatatattcc
    36301 ggcaccttcg ctacataaaa ggtgccgaaa ataacgcaag aaattatttt tccggcagtt
    36361 ttatatcttt aaacatgacc tcaatttctt cattggagcg caatgcgaca gccgcatcaa
    36421 cgacatcacg cgttaaatgc ggtgcaaaac gctgaatgaa atcatacata taactgcgca
    36481 aaaaagtact acggcgaaaa ccaattttgg ttgtactgtg gctgaagata tcgtgagcat
    36541 caacgcgcac aaggtcggga tcggcgaccg gatccaccgc catgctggca atgaccccta
    36601 cccccagccc taaccggacg taagttttaa tgacgtcagc atccgttgcc gtgaaaacga
    36661 tacgcggcgt taaccctgcg cggttaaagg cagtatccag ttctgaacgt ccggtaaagc
    36721 cgaaggtata tgtcaccaac ggatattgcg ccagttcttc aatggtaatg gcttttttgc
    36781 ctgccagcgg gtgatccgga gtgactacaa tagcccgatt ccagtggtag cacggtaaca
    36841 tcactaaatc ttcatacaga tgcagcgctt ctgtggcgat agcgaaatcg gcattgcctt
    36901 tagagacggc atcagcaatt tgtgtcggcg ggccctggtg catatgcaaa gaaacgcggg
    36961 gataacgctc aataaagcct ttgataacgt ttggtaacgc gtagcgtgcc tgggtatgcg
    37021 tggtggcgat atacagcgag cctttatccg gccaggtgtg ctcaccggca accgatttta
    37081 tggcatcgac tttcgacagg acttcgcgag cgatacgaat tatttcttgt cctgctggcg
    37141 ttacctgcgt caggtgcttg ccgcttcggg aaaaaatttg aatgcctagc tcgtcttcca
    37201 gcattctgac ttgtttactg atcccgggtt gtgatgtgta aagtccttcc gctgttgatg
    37261 agacattcag gttatgattg accacctcaa caatatagcg aagttgttgt aatttcatgt
    37321 taaaccatcc acttagacgc catcagaaca tcgttttttc gtcacacctg gcgggtgact
    37381 aatacgattt aataataaaa aaggtgctaa ctataaccac tatatcattt atagccttag
    37441 tgtataggtg tgaagaaaaa ataatatgca ataaaaaagg gccgctttcg cgaccctttg
    37501 tttataaaaa cctgacagaa ttaaaggtta tttttttcct tcaacccatt tgccatcaac
    37561 ataaaatgct gaccagccag tcgcctttcc gtctttttcc gaagagacat attgctgttt
    37621 ggttttacgg ctaaagcgaa ccatggtctt attaccttcc ggatcctgct gtggcgcatc
    37681 ggccagataa cgcagttttt ccggcagacg gtcgcggaag cgataaagct cttccaccag
    37741 tggcgcacgc gtttcacgcg atttcgggaa agtgttggca gccaggaaca caccggcagc
    37801 accgtcacgc agcacgaaat aagcatctga tttttcgcac ggcagctcag gtaatggcac
    37861 cggatcttct ttcggtggtg ccacttcacc gttacgtaaa atcttacgtg tgtttttaca
    37921 ctcttcgttg gtgcaggcca tgtatttacc gaaacgcccc attttcaggt gcatttcaga
    37981 gccacatttt tcacactcaa cgatcgggcc gtcataacct ttaatgcgga attcgccctc
    38041 ttcgatctcg taaccgtcgc aggttgggtt attaccacag acatgcaact tacgtttcgg
    38101 atcgatgaga tagctgtcca tcgccgtgcc gcatttcggg caacgacgtt ttgcgcgcag
    38161 cgcgttggtt tcagcatctt cgccttccag cacgttcagc acttcgtttt ccggcaccag
    38221 gttaatggtg gttttgcaac gctctttcgg cggcagcgca tagccagaac agccaaggaa
    38281 taccccggta ctcgctgtgc gaatccccat tttgcgacca caagtcgggc agtcaatgct
    38341 ggtcagaacc atctggttcg ggcgcatacc accctcttcc ggatcttttt cagctttatc
    38401 taactgctgg gtgaaatccg agaagaagtg atccagtaca gctttccact ctgcttcgtg
    38461 atttgccacc tggtcgaggc tgttttccat ctgcgcggta aagtcgtagt tcattaactc
    38521 gcggaaattt tcttcaaggc gatcggtgac gatttcgccc attttttccg catagaaacg
    38581 acgattttct actcgcacat agccacgatc ctgaatggtc gaaatgatcg acgcataggt
    38641 agacggacga ccgataccac gtttttccag ttctttaacc agcgatgctt cactgaaacg
    38701 ggctggcggc ttggtaaagt gctgggctgg tgttagttca acgagcgtca gagcatcgcc
    38761 tttatcgact gccggtaaga tacgatcttc atcgccttta cgcagtgcag gcatcacttt
    38821 tgtccagcca tcaaagcgca aaatacgacc gcgtgctttc aggcggaaat cgcccgcacc
    38881 aacggtcagc gtcgtggagt catatttcgc tggggtcatc tggcaggcaa cgaactgacg
    38941 ccagattaac tggtacagtt tctgcgcatc tgcttccata tccttcagcg attccgccat
    39001 cacattgacg tcagaaggac gaatcgcttc gtgcgcttcc tgtgagtttt ctttgctggc
    39061 gtactgattc ggactttccg gcagatattt cttaccaaaa ttatcgctga tataaccgcg
    39121 aaccatattt accgcgtcct gactcaggtt agtggagtcg gtacgcatgt aagtgatata
    39181 gcctgcttca tacaaacgct gcgccatcat catggttttt ttcacgccaa aaccaagacg
    39241 ggtgctggca gcttgttgca gcgtagaggt aataaaagga gcgccaggtt tactggttgt
    39301 cggtttgtct tcacgttcca gcacgctgta gcgcgctttt tccagcagac ttaccgcagc
    39361 ctgagtttgt tctttgttga ccggacggaa cggtttgtca ttctgatgag tcacctgcaa
    39421 ctccaacgct tcaccagatg gcgtagtcgt gctggcatcg acttcccaga actcttccgg
    39481 tacgaacgct ttaatttcac gctcacgctc taccaccagg cggaccgcca ccgactgcac
    39541 acgaccggca gacagaccac gagcgatctt tttccacagc agcggcgaaa ccatataccc
    39601 caccacgcgg tccataaagc gacgcgcctg ctgggcatta acacgatcaa tattcagctc
    39661 acccggtttg ttaaatgcct ggcggatcgc gtttttagta atttcgttaa acaccactcg
    39721 gctatagcgc gcatcatcac caccaatcac ttcccgcagg tgccatgcaa tggcttcccc
    39781 ttcgcggtca aggtcggttg cgagatagat gtggtcggct ttttcagcca gttgtttcag
    39841 ttcagagacg accttctctt taccaggcaa cacttcatag tgcgcctccc aattgtgcca
    39901 cgggtcaacc cccatacggt tgacgagagc gccacgttca tcctttttag gctttttagc
    39961 cgtcttggtg gaggtagagt cggcactctt tttggcagct gagccactgg tcggcaaatc
    40021 gcggatgtga ccgacgctgg atttcaccac gtagtcacaa cccagatact tgttgatcgt
    40081 tttggctttt gccggggact caacgatgac aagagcttta cccatattca ccttacctaa
    40141 tttgattctt ccaggaatgc gtcgcacgtt gataaacctt ccactggcaa cgagttaccg
    40201 atatggactc gctctctgcg gatatcaacc ccttgtcgtg actctgttca cgaaaattca
    40261 ggtgactgag ttaacaggtc ttggcctaca ggcgctatag cacgacggat ttttggtcga
    40321 atgtcaagca aatctattgc cagattgacg aaagcgtcac actgtacctg ataaaatccg
    40381 atacgcaact ttattagcat gcaaaatcca aagttgccag taaggcgctc ttgccaccag
    40441 cgtggtatat ttgtctgcaa gccatgcctg gcgtagacta atgtccttgt ttaaggagta
    40501 aataatgaac aaagagacac aacccatcga ccgcgaaacg ctgttgaaag aggccaacaa
    40561 aatcattcgt gagcatgagg atacgctcgc agggattgaa gccaccggcg tcacgcagcg
    40621 taatggcgtg ctggtattta ccggcgacta ctttttagat gaacaaggat tgccaacagc
    40681 caaaagcacc gcggtgttta atatgttcaa acacctggcc catgtgcttt cagaaaaata
    40741 tcatctggtt gattaaagca aaacgcgagg tcttagcctc gcgtttgtct tttacatcaa
    40801 tggcttttga ccccgctgcc accagcgtag caacaatcga tcggcgctct ctgccgcgct
    40861 gccggtgaat cggtcaatga gtcgtttacg ctgcatatag cgtacattaa ccacttcacg
    40921 gccttccatc aggctaagaa taacttcatc actggtgttg atttcatcaa ccaggccttt
    40981 ctctaccgcc tgttgtccgt accagtgttc acccgttgcc acctgttcaa tatccagaga
    41041 cggacgcata cgcttcacaa aatctttaaa taactgatgc gtttcgttca gctcttcgcg
    41101 gaatttctcc cgcccttctt cggtattttc acccagcaag gtcagcgtgc gcttatactg
    41161 cccggcggtg tgcagttcga tatcaatatc tttgcttttc aggaagcggt taaagttggg
    41221 catttgcgcc accaccccaa tggaacccac aatagcaaac ggtgcggaaa caattttgtc
    41281 cgccacacag gccatcatgt aaccgccgct ggcagcgact ttgtctaccg taacagttaa
    41341 aggaatgttt ttatcacgca gacgctgcag ctgcgaagcc gccaacccgt aaccatgcac
    41401 catgccacca gggctttcca gacggagcac aacctgatcc tgcggtttga atgctgcgag
    41461 aaccgccgtt atctcttcac gtagcgagtt cacttcatgg gcgtccatgc tgcctttaaa
    41521 atccagcacc cagacgcgag gtttactgtc agttgccacc tcgcccagtt tggctttcgc
    41581 ttttgctgct ttcgcttctt gcttgtgctt tttcttctgc gctttgtgcc actgtttttg
    41641 ctgatgtgag tccatcagcg ccgcggccag ttcttctttc atctccttat actgttcgct
    41701 gagattgttg acccgtaact cgccacgctg gcgtttatta cgttgagcaa cattgacaat
    41761 aatggcggca atcgccgcaa tcgctagcac aacggtaacg attttagcca aaaacaaacc
    41821 atattcagac aacaattcca cgagtcccca ccttggttaa acaacgcttt ggctcacagt
    41881 ttacaacagg catgttaagc cgtctcgcag agatgttatt gcagcgaaag tatgacaaag
    41941 tatccatttt gcggtgttat cgccccaggc tgattgaaat gcccttctgt ttcaggcata
    42001 aagccccaaa gtcataaagt acactggcag cgcgacagac aacgtcgttg aggagtagcc
    42061 atgcattacc agccaaaaca agatttactc aatgatcgca ttatcctggt gacgggagcc
    42121 agcgatggga ttggtcgtga agccgcgatg acgtatgcac gctatggtgc gacagtgatt
    42181 ctgttgggcc gtaatgaaga aaaattacgt caggtagcca gccacataaa cgaagaaact
    42241 gggcgtcagc cacagtggtt tattctcgat ttgctgacct gcacgtccga aaattgccaa
    42301 caactggcac agcgcattgt cgttaattat ccgcgtctgg atggtgtttt gcataatgcc
    42361 ggattgctcg gcgatgtttg cccaatgagc gaacaaaatc cgcaggtctg gcaggacgtc
    42421 atgcagatca acgttaatgc cacctttatg ctcacccagg cactgcttcc tttattactc
    42481 aaatcggacg ccggttcact ggtctttact tcatcaagcg ttggacgtca gggacgagcc
    42541 aactggggtg catatgcagc gtcgaaattt gccaccgaag ggatgatgca ggtactggcc
    42601 gatgaatatc agcagcgcct gcgcgtcaac tgcattaacc caggcggtac gcgcaccgca
    42661 atgcgcgcaa gcgccttccc gaccgaagat ccacagaaac ttaaaacacc cgctgatatc
    42721 atgccgctct acctctggct gatgggcgat gacagccgcc gtaaaaccgg catgaccttt
    42781 gacgcccaac cgggccgtaa accaggaatt tcccaatgag tgatgaacgc taccaacagc
    42841 gtcagcagcg agtgaaagaa aaagtagatg ctcgtgtggc ccaggcccag gatgaacgcg
    42901 gtattatcat cgtctttacc ggcaatggaa aaggcaaaac caccgcggca tttggtacgg
    42961 caacacgcgc agttggtcac ggaaaaaaag tgggcgtcgt gcagtttatt aaaggcacct
    43021 ggcctaatgg cgaacgcaat ctgctggagc cacatggcgt tgagtttcag gtgatggcaa
    43081 cgggctttac ctgggataca caaaaccgcg agtctgatac cgccgcctgc cgcgaagtct
    43141 ggcaacatgc aaagcggatg cttgctgatt cctcactgga tatggttttg cttgatgaac
    43201 tgacgtatat ggtggcgtat gactatttgc cactggaaga agtggtgcag gcgttaaatg
    43261 aacgtccaca tcaacagacg gtgattatca cgggtcgagg ctgtcatcgg gatattcttg
    43321 aactggcgga tacggtaagt gagctgcgcc ccatcagaca tgcgtttgat gccggtgtta
    43381 aggcgcaaat agggattgat tattaatctg taaaaatgcc ggatatgacg tccggcatac
    43441 tttgattaac cgttattacg cccgccagaa cgacgactgc cgctcacctg actgtgacgt
    43501 ttcaccgcac gacgaatctg attagccttc atgcgacgac ggtctttttc tacagcgact
    43561 ttagagctgg tttctggcgg aagctccacc agttcgcgca gatagttagt ctgggcgagg
    43621 tccagctccg tccagccacc gcgtggcaga ccttttggca gtgggatatc accgtaacga
    43681 acacggatca ggcggctcac ctgcacacca accgcttccc acagacgacg aacttcacgg
    43741 ttacggcctt cggtcagagt cacgttgtac cactggttga tcccttcgcc gccgctgaac
    43801 ttgatggttt taaaagctgc cggaccatct tccagttgca cgccacgact caaatcacgc
    43861 agtttcgcgt cgtcaacctg accaaataca cgcacggcgt attcacgttc aacttcacgg
    43921 cttggatgca ttaaacggtt tgccagttca ccatcggtgg tgaacagcag cagaccacag
    43981 gtgttaacgt ccagacgacc tacggcaatc cagcgtgcgc cacgcagctt tggcagacgg
    44041 tcaaacaccg tcggacgccc ttccggatcg ttacgggtac acaactcacc ttccggtttg
    44101 taataggcca gaacgcgaca aatttgttca gcggactcac gtaccgaaat cagatgacca
    44161 tcgatacgaa ttttcagccc aggggtaact tcaacgcgat cgccgagttt agcaattttg
    44221 ccatcaacac tcacacgacc ggcttcaata atagattcga tttcacggcg ggagccgtgg
    44281 ccagcgcggg ccagcacttt ctgtaatttt tcgctcatag agcttccttt tggtgtcgcc
    44341 ttcacaggcg tcgaacagga gaatcaatgg cgccgtatag cgccatttta aggccgcgta
    44401 gtatacagag ttacgcactt ataagaaagg cttcacatca cctacgcctt cacgcaccac
    44461 gaccggcgta tcatcggtaa gatcaataac cgttgttggt ttctgcccga gatagccgcc
    44521 gtgaataatc aaatctacct gtttttccag acggtcttta atttcttcgg gatcggattc
    44581 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
    44641 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttctc
    44701 ctgcaacaga cggcgtggca cttccttcgt ccccttcagg atgaaggtat agttgcccgg
    44761 cgtgttgttt ttcatcaaac gaaacgcaac gttatccacg aatgaatagg tcgagagttc
    44821 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
    44881 aatacgctcc atggcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
    44941 tggatagaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
    45001 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
    45061 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
    45121 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
    45181 tgctgacgcg caagtgtggc cagctgggtg cgttcattgg gcgattgctg gcactgcgcg
    45241 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
    45301 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
    45361 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
    45421 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc cacaaggaaa
    45481 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    45541 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    45601 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    45661 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    45721 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    45781 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    45841 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca
    45901 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    45961 aacgggtctg gcaaatattc tgaaatgagc tgttgacaat taatcatcga accagttaac
    46021 tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagg
    46081 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    46141 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    46201 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    46261 tttttcacca gttgtgtggg aatcgtccgg caacgctgct gctggaatcc gcagatatcg
    46321 acagcaaaga tgatttaaaa agcctgctgc tgatagacag tgcgctgcgc attacagcat
    46381 taggtgacac cgtcacaatt caggcacttt ccggcaatgg cgaagccctg ctggcactac
    46441 tggataacgc cctgcctgcg ggtgtggaaa gtgaacaatc accaaactgc cgtgtgctgc
    46501 gcttcccccc tgtcagtcca ctgctggatg aagacgcccg cttatgctcc ctttcggttt
    46561 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    46621 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    46681 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    46741 ttgaccatca gaaaaaaagc acccgtattc aggccagcct gtttgctccg aatgaagaag
    46801 aaaaacaacg tctcactgct cgcctgaacg aactacgtca gcaactgacc gaagccgcgc
    46861 cgccgctgcc agtggtttcc gtgccgcata tgcgttgtga atgtaatcag agcgatgaag
    46921 agttcggtgg cgtagtgcgt ttgttgcaaa aagcgattcg cgctggagaa attttccagg
    46981 tggtgccatc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    47041 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctat
    47101 ttggcgcgtc gccggaaagc tcgctcaagt atgatgccac cagccgccag attgagatct
    47161 acccgattgc cggaacacgc ccacgcggtc gtcgcgccga tggttcactg gacagagatc
    47221 tcgacagccg tattgaactg gaaatgcgta ctgaccataa agagctgtct gaacatctga
    47281 tgctggttga tctcgcccgt aatgatctgg cacgcatttg cacccctggc agccgctaca
    47341 tcgccgatct caccaaagtt gaccgttatt cctatgtgat gcacctcgtc tctcgcgtag
    47401 tcggcgaact gcgtcacgat cttgacgccc tgcacgctta tcgcgcctgt atgaatatgg
    47461 ggacgttaag cggtgcgccg aaagtacgcg ctatgcagtt aattgccgag gcggaaggtc
    47521 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    47581 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagcgggtg
    47641 ctggtgtagt ccttgattct gttccgcagt cggaagccga cgaaacccgt aacaaagccc
    47701 gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    47761 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    47821 caatggtcat aacgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    47881 cctggcgacg atgagcaatc cggtactgat gctttctcct ggccccggtg tgccgagcga
    47941 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    48001 ttgcctcgga catcaggcaa ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    48061 aattcttcac ggtaaagcgt cgagcattga acatgacggt caggcgatgt ttgccggatt
    48121 aacaaacccg ctgccggtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    48181 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    48241 cgtttgtgga ttccagttcc atccggaatc cattctcacc acccagggcg ctcgcctgct
    48301 ggaacaaacg ctggcctggg cgcagcagaa actagagcca gccaacacgc tgcaaccgat
    48361 tctggaaaaa ctgtatcagg cgcagacgct tagccaacaa gaaagccacc agctgttttc
    48421 agcggtggtg cgtggcgagc tgaagccgga acaactggcg gcggcgctgg tgagcatgaa
    48481 aattcgcggt gagcacccga acgagatcgc cggggcagca accgcgctac tggaaaacgc
    48541 agcgccgttc ccgcgcccgg attatctgtt tgctgatatc gtcggtactg gcggtgacgg
    48601 cagcaacagt atcaatattt ctaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    48661 agtggcgaaa cacggtaacc gcagcgtctc cagtaaatcc ggctcgtcgg atctgctggc
    48721 ggcattcggt attaatcttg atatgaacgc caataaatcg cgccaggcgc tggacgagtt
    48781 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga ttccgccacg cgatgccggt
    48841 tcgccagcaa ctgaaaaccc gcaccctgtt caatgtgctg gggccattga ttaacccggc
    48901 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    48961 aaccttgcgc gtgctggggt atcaacgcgc ggcggtggtg cacagcggcg ggatggatga
    49021 agtttcatta cacgcgccga caatcgttgc cgagctgcat gacggcaaaa ttaagagcta
    49081 tcaattgacc gctgaagatt ttggcctgac tccctaccac caggagcaac tggcaggcgg
    49141 aacacctgaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    49201 ccatgaagca gccgtcgctg cgaacgtcgc catgttaatg cgcctgcatg gccatgaaga
    49261 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    49321 agttaccgcg ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    49381 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    49441 tgaggttcag ccgagcacac gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    49501 tattctggag tgtaaaaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcgatcc
    49561 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    49621 gaaatatttt caggggagct ttgatttcct cctcatcgtc agccaaatcg ccccgcagcc
    49681 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    49741 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    49801 agccgtcgct cacagtctgg agatgggggt gctgaccgaa gtcagtaatg aagaggaact
    49861 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    49921 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    49981 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    50041 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    50101 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    50161 agcttatgac gcgggcgcga tttacggtgg gttgattttt gttgcgacat caccgcgttg
    50221 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    50281 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    50341 agtgcaactg catggcaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    50401 agcacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    50461 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    50521 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    50581 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    50641 agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    50701 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    50761 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    50821 agcgcgcaaa aagatcctga atttcaagcc cagttcaacg acctgctgaa aaactatgcc
    50881 gggcgtccaa ccgcactgac caaatgccag aacattacag ccgggacgaa caccacgctg
    50941 tatctcaagc gtgaagattt gctgcacggc ggcgcacata aaaccaacca ggtgctgggg
    51001 caggcgttgc tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggggccggt
    51061 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    51121 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    51181 gcggaagtga tcccggtaca tagcggttcc gccacgctga aagatgcctg taatgaggcg
    51241 ctacgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcggctggc
    51301 ccgcatcctt atccgaccat tgtccgtgag tttcagcgga tgattggcga agaaaccaaa
    51361 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    51421 ggttcgaatg ccatcggcat gtttgctgat ttcatcaatg aaaccaacgt cggcctgatt
    51481 ggtgtggagc ctggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    51541 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    51601 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg tccacaacac
    51661 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    51721 gaagccttca aaacgctgtg cctgcacgaa gggatcatcc cggcgctgga atcctcccac
    51781 gccctggccc atgcgctgaa aatgatgcgc gaaaacccgg ataaagagca gctactggtg
    51841 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    51901 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    51961 aaggcgcatt cgttcctttc gtcacgctcg gtgatccggg cattgagcag tcattgaaaa
    52021 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    52081 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    52141 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    52201 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    52261 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    52321 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    52381 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacatatt
    52441 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    52501 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    52561 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    52621 ccattgttaa aatcatcgag caacatatta atgagccaga gaaaatgctg gcggcactga
    52681 aagtttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc cgccagttcc
    52741 gctggcggca ttttaacttt ctttaatgaa gccggaaaaa tcctaaattc atttaatatt
    52801 tatcttttta ccgtttcgct taccccggtc gaacgtcaac ttacgtcatt tttccgccca
    52861 acagtaatat aatcaaacaa attaatcccg caacataaca ccagtaaaat caataatttt
    52921 ctctaagtca catattcctc aggtaattgt taatatatcc agaatgttcc tcaaaatata
    52981 tttccctcta tcttctcgtt gcgcttaatt tgactaatcc tcattagtga ctaattttaa
    53041 tgagtgtcga cacacaacac tcatattaat gaaacaatgc aacgcaacgg gagaaataac
    53101 atggccgaac atcgtggtag ttcaggaaat ttcgccgaag accgtgagaa ggcatccgac
    53161 gcaggccgta aaggcggtca gcatagcggc ggtaatttta aaaatgatcc gcaacgcgca
    53221 tctgaagcgg gtaaaaaagg cggtcaacaa agcggtggta ataaatcagg caaatcctga
    53281 ttcaccgtta attattaata tcagctgata aagataattg catctgcggg ccgtatggct
    53341 cgcagtgatt tcactggaga aaaatatgaa tatgaagacc attgaagatg tatttattca
    53401 actgctttca gatacctaca gcgcagaaaa acaattaacc cgggcactgg caaaactcgc
    53461 aagagcaaca tcaaatgaaa aattaagtca ggcttttcat gcgcacctcg aggaaactca
    53521 tggacagatt gaacgaattg atcaagttgt ggaatcagaa tcaaatctga aaattaagcg
    53581 catgaaatgt gtggcaatgg aaggtcttat tgaagaagct aatgaggtca tcgaaagtac
    53641 cgagaaaaac gaagtgcgtg atgccgcact gattgccgca gcacagaaag tcgagcatta
    53701 tgagattgcc agttacggga cattagcgac gctggctgaa caattaggtt accgtaaagc
    53761 agcgaagctt ctgaaagaaa ccctggaaga agaaaaggcc accgacatca aactgactga
    53821 tctggccctt aataacgtaa ataagaaagc cgaaaataaa gcctgaaata tgaattttaa
    53881 cttttagtca ttttataaag aggacatttt catgaatcgt attgaacatt atcatgactg
    53941 gttacgtgac gcccacgcaa tggaaaagca agccgaatct atgcttgaat ccatggccag
    54001 ccgtatagat aattatcctg aactacgcgc tcgtattgaa caacatctta gtgaaaccaa
    54061 aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac gttcagtcat
    54121 taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta tattcccttc
    54181 tgatgaaata gttaaaggct ctattggcgg atatgtcttc gagcaatttg aaatcacctg
    54241 ttacacctca ctattagcag cagcaaaaaa tgccggtgat acagtctcaa ttccgatcat
    54301 cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc atattccgca
    54361 aacaactgag aaatttttaa ttcgttctga aactgatggc gtagaagcga agaaataata
    54421 ccctttatac catgtcctta ttgaccccgt atattacggg gtcgtttttg tgcggaatta
    54481 aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc tatcgtgttg
    54541 ctgtgcaccg cctagcttat aattggcggt ggtatcgata tccatgtacc acactgacat
    54601 gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg cagctcccca
    54661 ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt taaatccatt
    54721 atcaaagaag gtggtgtagt taataccggc cccaacgtaa ggacggaatt tgctgctggc
    54781 atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tggtgaacgg ttgcaatatc
    54841 gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta attccacacc
    54901 aatgttgtcg gtcgccatat aagtaaacgt caagcccagt tgagtgttat tggtcacgct
    54961 gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta cggttgcaga
    55021 acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag gcactgccag aaagaagagt
    55081 tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt tatggttata
    55141 aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga tcacattaca
    55201 tatgataatt caccatttat tgatctggat taatttagaa gttgcattga gaatcctggt
    55261 taatgtgatt taaatcaatt tttaaatatt ttctctacgc aaatattaac tcgatcgcca
    55321 cttgccgcgc tttgcccacg cctgaaggcc agcaattacc tccgctttac aaagatatgc
    55381 ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa ttgcccgcta
    55441 attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag tccgtatacc
    55501 gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta tcgttgctat
    55561 gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca cagcttgcgc
    55621 agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg gttcagaata
    55681 tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact ttttcagaat
    55741 taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg gtgtctgcgg
    55801 gtcagagagt cagtgcgctg cgagctattg gtgccagtgc gccgatattg cccaagttat
    55861 ttattctgat ttttctgact acccttttag tacagattgg catcatgctg gtggtcgttc
    55921 cgggaattat catggccatt ttactggccc tggcaccggt gatgctggta caggacaaaa
    55981 tgggcatttt tgcctcgatg cgtagcagta tgcggctgac ttgggcgaat atgcgtctgg
    56041 tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt tttgcctctt
    56101 cttttgccgc attaaccccg gaaattggtg ccgtactggc gaacaccttg agcaacctga
    56161 tttctgccat attgctcatc tatctgttcc gcctgtatat gttgattcgc caataacctt
    56221 aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac cgctggttgt
    56281 ctttttcgcg ttttacaaga tttatgacat ctatgcggct actgcagcgc tgatcgtcgc
    56341 cacggcgatt gtgcttatct atagctgggt tcgctttcgt aaggttgaga agatggccct
    56401 gatcactttt gttctggtgg tcgtcttcgg tggcttgacg ctgttcttcc acaatgatga
    56461 gtttattaaa tggaaggtta cagtcattta tgctctgttt gcgggagccc tgttagtcag
    56521 ccaatgggtg atgaaaaagc cgctaattca gcggatgctg agtaaagaac tcacgctgcc
    56581 gcaaccggta tggtcgaagc taaatctggc ctgggctgtt ttctttatcc tttgcggtct
    56641 ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact ttaaagtctt
    56701 tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata tctaccgcca
    56761 catgccgcag gaagataaat cctaaccaga ctgccagtcc ttcggggctg gcgctgtatc
    56821 tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc cttttaagtc
    56881 ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt acgtacttta
    56941 gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt aatgtcacaa
    57001 atggatattg gcggcgctat tctggcaaaa gaaattgccc acggtcgcgt agtgactgtg
    57061 cgggttgaag gaatgacttt cttacggccg gttgcggtcg gcgatgtggt gtgctgctat
    57121 gcacgctgtg tccagaaagg gacgacatcg gtcagcatta atattgaagt gtgggtgaaa
    57181 aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga cagaagcatt atttaagtat
    57241 gtagcggttg atcctgaagg aaaacctcgc gccttacctg ttgagtaata gtcaaaagcc
    57301 tccggtcgga ggcttttgac tttctgctta ctgaatttcg gtggtgccgt taattttaaa
    57361 caggatattc accacaatcc cactgcctgg cttacccggc tcataacgcc atctgcgcat
    57421 cgcatttttc acctcacgct caaacatgtt cgcaggcttg gctgagagga tttgtacgtt
    57481 atccacgcga ccatccggcg tgacgtcaaa tttaacttta acctgccctt caatgcgcaa
    57541 tgcctgtgct cgtgccggat actgcggctg attacggctt aatgcgcgtg gtcctgaagc
    57601 cacactggta accggcttgc tggttgcagc cgttgccgta cttgatgtgg ggcgcgcggg
    57661 tgccgtattt tcaaacggtg atgccggacg cgactctacg ggtttgacat cgcgttttgg
    57721 ctgctcctgt acctttttca ccggcttcgg ttttggctta ggtttcggct tcggcttttc
    57781 aatgaccacc ggtgcttctt ttggcggttc ggggatcggc tcaggttccg gttctggctc
    57841 taccaccggc tccggtggcg gctgaacggc ttgtggcggt tcgagatcag caggcgcaac
    57901 catcgtgaca gaaattggtt gcgcaggcgc aggtagttca ataacctgat gtaccgaggt
    57961 atagagcagc cccgccacaa cagcaccatg aatgcagacc gaaagtaacg tcggccaggg
    58021 gaagcggcga ggtaaatcaa gggttattga agtcataatc atttcagtag aaaaactagg
    58081 cctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc aggatcagtg
    58141 aaaatgtcat ttttggcgta attaccctta ctttatgcat gaccttaaca aagacatcat
    58201 ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc ccgaacaagg
    58261 agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc tcgaaaaacg
    58321 cc
//
LOCUS       DAJTRW010000014        58000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRW010000014 DAJTRW010000000
VERSION     DAJTRW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(24..278)
                     /locus_tag="OO326_000934"
     CDS             complement(24..278)
                     /locus_tag="OO326_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5965420.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO326_000935"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO326_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5965421.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO326_000936"
     CDS             1228..2694
                     /locus_tag="OO326_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5965422.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO326_000937"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO326_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5965423.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO326_000938"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO326_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5965424.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO326_000939"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO326_000939"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5965425.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO326_000940"
     CDS             5709..7265
                     /locus_tag="OO326_000940"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5965426.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO326_000941"
     CDS             7347..9161
                     /locus_tag="OO326_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5965427.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO326_000942"
     CDS             9162..10256
                     /locus_tag="OO326_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5965428.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO326_000943"
     CDS             10256..11281
                     /locus_tag="OO326_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5965429.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO326_000944"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO326_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5965430.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREEARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO326_000945"
     CDS             complement(12876..13220)
                     /locus_tag="OO326_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965431.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO326_000946"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO326_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5965432.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO326_000947"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO326_000947"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5965433.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO326_000948"
     CDS             15616..17376
                     /locus_tag="OO326_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5965434.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO326_000949"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO326_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5965435.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO326_000950"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO326_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5965436.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO326_000951"
     CDS             19113..19340
                     /locus_tag="OO326_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5965437.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO326_000952"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO326_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5965438.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO326_000953"
     tRNA            21195..21271
                     /locus_tag="OO326_000953"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO326_000954"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO326_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5965439.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO326_000955"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO326_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5965440.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO326_000956"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO326_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5965441.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO326_000957"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO326_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5965442.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO326_000958"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO326_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5965443.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO326_000959"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO326_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5965444.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO326_000960"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO326_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5965445.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO326_000961"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO326_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5965446.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO326_000962"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO326_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5965447.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO326_000963"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO326_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5965448.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO326_000964"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO326_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5965449.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO326_000965"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO326_000965"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5965450.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO326_000966"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO326_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5965451.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO326_000967"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO326_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5965452.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO326_000968"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO326_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5965453.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO326_000969"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO326_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5965454.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO326_000970"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO326_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5965455.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO326_000971"
     CDS             37391..37555
                     /locus_tag="OO326_000971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965456.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO326_000972"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO326_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5965457.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO326_000973"
     CDS             complement(38300..39331)
                     /locus_tag="OO326_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5965458.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO326_000974"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO326_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO326_000975"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO326_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5965459.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO326_000976"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO326_000976"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5965460.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO326_000977"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO326_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5965461.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO326_000978"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO326_000978"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5965462.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO326_000979"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO326_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5965463.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO326_000980"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO326_000980"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5965464.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO326_000981"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO326_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5965465.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO326_000982"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO326_000982"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5965466.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO326_000983"
     CDS             complement(53338..54114)
                     /locus_tag="OO326_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5965467.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO326_000984"
     CDS             complement(54119..55768)
                     /locus_tag="OO326_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5965468.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEEELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO326_000985"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO326_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="OO326_000986"
     CDS             complement(56332..56490)
                     /locus_tag="OO326_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965469.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58000)
                     /locus_tag="OO326_000987"
     CDS             complement(56487..>58000)
                     /locus_tag="OO326_000987"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5965470.1"
                     /translation="AQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRTSSSLSFI
                     SYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMP
                     DSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQ
                     DNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILMV"
BASE COUNT        14187 a        15647 c        14251 g        13915 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagagg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct cctcctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag
//
LOCUS       DAJTRW010000015        55639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRW010000015 DAJTRW010000000
VERSION     DAJTRW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..441)
                     /locus_tag="OO326_000988"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO326_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO326_000989"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO326_000989"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5965471.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO326_000990"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO326_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5965472.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO326_000991"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO326_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5965473.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO326_000992"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO326_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO326_000993"
     CDS             5754..6257
                     /locus_tag="OO326_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5965474.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO326_000994"
     CDS             complement(6337..6588)
                     /locus_tag="OO326_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5965475.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO326_000995"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO326_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5965476.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO326_000996"
     CDS             7052..7375
                     /locus_tag="OO326_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5965477.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO326_000997"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO326_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5965478.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO326_000998"
     CDS             complement(8080..8319)
                     /locus_tag="OO326_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5965479.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO326_000999"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO326_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5965480.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO326_001000"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO326_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5965481.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO326_001001"
     tRNA            complement(10643..10729)
                     /locus_tag="OO326_001001"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO326_001002"
     tRNA            complement(10742..10815)
                     /locus_tag="OO326_001002"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO326_001003"
     tRNA            complement(10870..10945)
                     /locus_tag="OO326_001003"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO326_001004"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO326_001004"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5965482.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO326_001005"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO326_001005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5965483.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO326_001006"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO326_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5965484.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO326_001007"
     CDS             14483..14659
                     /locus_tag="OO326_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965485.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO326_001008"
     CDS             14646..14870
                     /locus_tag="OO326_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5965486.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO326_001009"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO326_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO326_001010"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO326_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5965487.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO326_001011"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO326_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5965488.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO326_001012"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO326_001012"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5965489.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO326_001013"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO326_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5965490.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO326_001014"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO326_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5965491.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO326_001015"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO326_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO326_001016"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO326_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5965492.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO326_001017"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO326_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5965493.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO326_001018"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO326_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5965494.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO326_001019"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO326_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5965495.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO326_001020"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO326_001020"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5965496.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO326_001021"
     CDS             complement(26368..26781)
                     /locus_tag="OO326_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5965497.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO326_001022"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO326_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5965498.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO326_001023"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO326_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5965499.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO326_001024"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO326_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..30428)
                     /locus_tag="OO326_001025"
                     /pseudo
     CDS             complement(29220..30428)
                     /locus_tag="OO326_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30496..31111
                     /gene="tnpA"
                     /locus_tag="OO326_001026"
                     /pseudo
     CDS             30496..31111
                     /gene="tnpA"
                     /locus_tag="OO326_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31439..32521
                     /locus_tag="OO326_001027"
     CDS             31439..32521
                     /locus_tag="OO326_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5965500.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(33010..33808)
                     /locus_tag="OO326_001028"
                     /pseudo
     CDS             complement(33010..33808)
                     /locus_tag="OO326_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="OO326_001029"
     CDS             complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="OO326_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5965501.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="OO326_001030"
     CDS             complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="OO326_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5965502.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="OO326_001031"
     CDS             complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="OO326_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5965503.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            35743..37402
                     /gene="fliF"
                     /locus_tag="OO326_001032"
                     /pseudo
     CDS             35743..37402
                     /gene="fliF"
                     /locus_tag="OO326_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            37395..38390
                     /gene="fliG"
                     /locus_tag="OO326_001033"
     CDS             37395..38390
                     /gene="fliG"
                     /locus_tag="OO326_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5965504.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            38383..39069
                     /gene="fliH"
                     /locus_tag="OO326_001034"
     CDS             38383..39069
                     /gene="fliH"
                     /locus_tag="OO326_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5965505.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            39069..40442
                     /gene="fliI"
                     /locus_tag="OO326_001035"
     CDS             39069..40442
                     /gene="fliI"
                     /locus_tag="OO326_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5965506.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            40464..40805
                     /gene="fliJ"
                     /locus_tag="OO326_001036"
                     /pseudo
     CDS             40464..40805
                     /gene="fliJ"
                     /locus_tag="OO326_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            40802..41929
                     /gene="fliK"
                     /locus_tag="OO326_001037"
     CDS             40802..41929
                     /gene="fliK"
                     /locus_tag="OO326_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5965507.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            42034..42498
                     /gene="fliL"
                     /locus_tag="OO326_001038"
     CDS             42034..42498
                     /gene="fliL"
                     /locus_tag="OO326_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5965508.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            42503..43507
                     /gene="fliM"
                     /locus_tag="OO326_001039"
     CDS             42503..43507
                     /gene="fliM"
                     /locus_tag="OO326_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5965509.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            43504..43917
                     /gene="fliN"
                     /locus_tag="OO326_001040"
     CDS             43504..43917
                     /gene="fliN"
                     /locus_tag="OO326_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5965510.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            43920..44285
                     /gene="fliO"
                     /locus_tag="OO326_001041"
     CDS             43920..44285
                     /gene="fliO"
                     /locus_tag="OO326_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5965511.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            44285..45050
                     /gene="fliP"
                     /locus_tag="OO326_001042"
                     /pseudo
     CDS             44285..45050
                     /gene="fliP"
                     /locus_tag="OO326_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            45060..45329
                     /gene="fliQ"
                     /locus_tag="OO326_001043"
     CDS             45060..45329
                     /gene="fliQ"
                     /locus_tag="OO326_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5965512.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            45338..46123
                     /gene="fliR"
                     /locus_tag="OO326_001044"
     CDS             45338..46123
                     /gene="fliR"
                     /locus_tag="OO326_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5965513.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            46408..47031
                     /gene="rcsA"
                     /locus_tag="OO326_001045"
     CDS             46408..47031
                     /gene="rcsA"
                     /locus_tag="OO326_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5965514.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(47075..47263)
                     /gene="dsrB"
                     /locus_tag="OO326_001046"
     CDS             complement(47075..47263)
                     /gene="dsrB"
                     /locus_tag="OO326_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5965515.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            47426..47653
                     /gene="yodD"
                     /locus_tag="OO326_001047"
     CDS             47426..47653
                     /gene="yodD"
                     /locus_tag="OO326_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5965516.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            47951..48766
                     /locus_tag="OO326_001048"
                     /pseudo
     CDS             47951..48766
                     /locus_tag="OO326_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(48763..50457)
                     /gene="yedQ"
                     /locus_tag="OO326_001049"
                     /pseudo
     CDS             complement(48763..50457)
                     /gene="yedQ"
                     /locus_tag="OO326_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(50628..50810)
                     /locus_tag="OO326_001050"
     CDS             complement(50628..50810)
                     /locus_tag="OO326_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5965517.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(50889..51806)
                     /locus_tag="OO326_001051"
     CDS             complement(50889..51806)
                     /locus_tag="OO326_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5965518.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            51979..52899
                     /gene="yedA"
                     /locus_tag="OO326_001052"
     CDS             51979..52899
                     /gene="yedA"
                     /locus_tag="OO326_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5965519.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(52888..53358)
                     /gene="vsr"
                     /locus_tag="OO326_001053"
     CDS             complement(52888..53358)
                     /gene="vsr"
                     /locus_tag="OO326_001053"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5965520.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(53339..54757)
                     /gene="dcm"
                     /locus_tag="OO326_001054"
     CDS             complement(53339..54757)
                     /gene="dcm"
                     /locus_tag="OO326_001054"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5965521.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(54824..55364)
                     /locus_tag="OO326_001055"
                     /pseudo
     CDS             complement(54824..55364)
                     /locus_tag="OO326_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(55404..>55639)
                     /locus_tag="OO326_001056"
     CDS             complement(55404..>55639)
                     /locus_tag="OO326_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965522.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT        13910 a        14324 c        13463 g        13942 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
    29701 gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat
    29761 ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa
    29821 ctgtttacag gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca
    29881 tccagcccga ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga
    29941 atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg
    30001 acctgacggc tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt
    30061 tcctgagcga gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg
    30121 aatcagggga acgccgagcg cttctggaaa aagtttttgc aggcctgctc agggtctttc
    30181 agtgactgtt gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt
    30241 ttccactcaa ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta
    30301 tgattctcat tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag
    30361 cgcctcattt cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt
    30421 cgtttcacag ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg
    30481 cactgtgttt tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt
    30541 tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg tgcttatttt
    30601 gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatctgtt aattaactat
    30661 ccccccaaac tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag
    30721 gttactgcga cgggatcgac cagatattgc tgccaggtat taccgcaaag atgttttgtg
    30781 gcgtcccggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    30841 cattgaacaa cagcaaacac ctggtcagat gaaaaaacga gccttatatc cccgccctga
    30901 aggacgggct tttacggtgc actggataag cctggagatt atctacacct gcatggacat
    30961 cattgtacta tgccccgtac atgctccatc acatcctcaa catcaaagta ttagagcctt
    31021 gcaaaccatt atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat
    31081 atcaagcggc gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa
    31141 ataaatatca tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa
    31201 attatgagtc aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg
    31261 ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta
    31321 cttcacaatt taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg
    31381 aatatttgta gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat
    31441 gaaaaaatta acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc
    31501 tcaggcagca gagatttaca ataaagacag taacaagctg gatctgtacg gaaaagttaa
    31561 tgctaagcac tacttctctt ctaacgatgc agatgatggc gacacaactt atgttcgtct
    31621 gggcttcaaa ggcgaaactc aaatcaacga tcaactgact ggtttcggtc agtgggaata
    31681 tgaattcaaa ggcaaccgtg ctgaatctca aggttcttcc aaagataaaa cccgtcttgc
    31741 cttcgctggc ctgaaattcg gtgactacgg ctccatcgat tatggccgta actacggtgt
    31801 agcatacgat atcggtgcat ggaccgacgt tctgccagaa ttcggtggcg atacctggac
    31861 ccaaacagat gtgttcatga ctggtcgcac cacaggtgtt gcaacctatc gtaacaacga
    31921 cttctttggt ctggttgatg gtctgaactt tgctgctcag tatcagggta aaaatgaccg
    31981 cactgacgta actgaagcta acggtgatgg tttcggtttc tccactactt atgagtatga
    32041 aggattcggt gtaggtgcaa cctatgctaa atctgaccgc actaatgatc aggttatcta
    32101 cggtaacaac agcctgaatg catctggtca aaatgctgaa gtatgggcag ctggtctgaa
    32161 atatgatgcg aacaacatct atctggctac cacctattct gaaacccaga acatgactgt
    32221 ttttggtaat aaccatattg ccaacaaagc acaaaacttc gaagtagttg cacaatatca
    32281 gttcgacttc ggtctgcgtc cgtccgttgc ttacctgcaa tctaaaggaa aagacttggg
    32341 tgcgtggggt gatcaggacc tgattgaata tattgatgta ggtgcaacct attacttcaa
    32401 caaaaatatg tccacttttg ttgattacaa aatcaacctg attgataaga gcgatttcac
    32461 gaaagcatct ggcgttgcta ccgatgatat cgttgctgta ggattggttt accagttcta
    32521 atctgattac tcaaagatat gttgtgggag gctttgcctc cccaacatat aagtggcgct
    32581 ctcaagccac tttccttaga agcactatct tgcttctgac tatataaatt accctttatt
    32641 ttctgggacg tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat
    32701 tagatttaat aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt
    32761 taattatccc ctcaacctct tgtaaatttc ccactcttga gctccacata gcccaaacgc
    32821 cggatgcaaa aaaaatcata cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa
    32881 ttatatgtgc ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca
    32941 ataggttgag tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat
    33001 aaaatacttt tactctctgg acgcttttct tagctctctt ggtgttatat taaaacgatt
    33061 atggaacctt tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct
    33121 gatgggtttt actgtcgttt gtatagcagg acaacgcatt atttaacctc acgtcgtcca
    33181 atatactttg aaaacttatt ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa
    33241 tgtagagata tctcgctacc ttgtttgctg tccattttgt tctaggttcg gatagcatta
    33301 ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa caattaacat
    33361 gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga
    33421 agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg gggttctgag
    33481 gtaagcagaa acctgtcgtg ttacgaattt gatcagttat cgcataaaat ttttgaaact
    33541 tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct gcttctggat
    33601 aattcacaat ataaaattca tatccacagg gaaataatat tatctgatta ttatcaacag
    33661 ttagactatg cgtctcccaa ttgataactt tttttccctg acggatacga cataaagctg
    33721 gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct
    33781 ttaggttaga cttatctctt gccagcatac tcttacccta ttttatctca taaactggtg
    33841 ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg
    33901 aattagaatc cacaggaatc ttatcagttt ttagagccca tactttaaac tggtaatgat
    33961 ggggtttatc tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc
    34021 ggccttgaac agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagtgggca
    34081 aatatgttac agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag
    34141 gagcatctgg atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc
    34201 aggttaatga gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg
    34261 ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcctgcg
    34321 cagaaaatgt tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat
    34381 tgttgtgacc tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca
    34441 atgatcgcgc aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca
    34501 caaatcagat tcctgttatc tcaaggagaa ttatttttta atgcggtgca cttcgtgaca
    34561 acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt
    34621 ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgactcctg
    34681 accagatagc ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga
    34741 atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg
    34801 aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat
    34861 aagggttcat agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac
    34921 acaactcacc gttttcatcc tggtgtatgt gtatccaagg ggctctggcg tggcaagaga
    34981 taaaggcagg aacaataatg atcagaagga accccaggct aacgaatgag ttcactcttc
    35041 ctgggagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa
    35101 caaccaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc catccatgcc
    35161 ccacgctgtg ggatcggata taattaaaca agcatagtca acaggttaca caacctacac
    35221 ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
    35281 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg
    35341 ttcaccgagg gtgaattttt ctgcctgcgt gcgggcgact gtttgtgtat cgcttatgcg
    35401 atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg
    35461 cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat
    35521 cgctgacatt ttcgtctccc ggataatttc tggtaacaaa gcctaccagt aagtcaataa
    35581 gacaaaggcg ctaaatagca acaaaaaaac tggtttattg gcggatagaa aaaacgaaag
    35641 cacaaataat gggagcgtca atttttcgag tgtgctgacc cgagagtgag tcttgttcca
    35701 ctttgccaat aacgccgtcc ataatcagcc acgaagtgcg cgatgaatgc gactgcagcc
    35761 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    35821 gttaccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
    35881 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
    35941 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaggttccg
    36001 gcagataaag ttcacgaact gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
    36061 ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
    36121 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
    36181 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
    36241 ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt
    36301 agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgcctgg aaacgtcacg
    36361 ctggtggatc agggcgggca tctgttaacc cagtccaata ccagcgggcg cgatcttaat
    36421 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcagcg tattgaagcg
    36481 atcctgtcac ctattgttgg taacggtaat attcacgcct aggtcacggc gcaactggac
    36541 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
    36601 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
    36661 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
    36721 ccgacaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag tgggccgcgt
    36781 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
    36841 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg
    36901 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agctctgacc
    36961 cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt taactcgccg
    37021 ttcaatagca gtgacgaaag cggcggagca ctgccgtttt ggcaacagca agtgtttatc
    37081 gatcagttgc tggctgccgg tcgctggttg ctggttgctg gtactgctgg tggcgtggtt
    37141 gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc gccgaggcgg tgaagactgt
    37201 gcagcaacag gcgcaggccc gcgaggaagt ggaagatgcg gtggaagtcc gtctgagcaa
    37261 agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg ggggcagaag tcatgagcca
    37321 gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg
    37381 gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc ctgctgatga
    37441 ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc gaagtgcaaa
    37501 ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc
    37561 tggcggagtt tgagcaagaa gctgaacagt ttgctgcact gaatatcaac gccaacgatt
    37621 atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc ctgctggaag
    37681 atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc
    37741 agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc attctggtgc
    37801 atctgaggcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg
    37861 acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gccgaactga
    37921 ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg
    37981 tgagaacggc agccgaaatt atcaacctga tgaaaacgca gcaggaagaa gccgttatta
    38041 ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag atgttcctgt
    38101 tcgagaatct ggtggatgtc gatgatcgca gcattcagcg tctgttgcag gaagtggatt
    38161 ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc
    38221 gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaag cgtggtccgg
    38281 tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct gctgattgtg cgccgtcttg
    38341 ccgaaactgg cgagatggtg attggcagcg gcgaggatac ctatgtctga taatctgccg
    38401 tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg cagagtttgt gccaatggtc
    38461 gagtcggaag aaaccattat tgaagaggcc gaacccagcc ttgagcagca actggcgcaa
    38521 ctgcaaatgc aggcccacga gcagggttat caggcgggga ttgccgaagg tcgccagcaa
    38581 ggtcacgaac agggctatca ggaaggactg gcccaggggc tggaacaagg tctggcagag
    38641 gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa
    38701 actacccttg atgcgcttga tagtgtgata gcttcgcgcc tgatgcagat ggcgctggag
    38761 gcagcacgtc aggtgatcgg tcagacacca acggtggata actcggcact gatcaaacag
    38821 atccaacagt tgttgcagca agaaccgtta ttcagcggga aaccacagct gcgcgtacac
    38881 ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ctttaagttt gcatggctgg
    38941 cgcttgcggg gcgatcccac cctccaccct ggcggatgta aagtctccgc cgatgaaggc
    39001 gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga
    39061 gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca
    39121 aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct accgggctgg
    39181 tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt gagcgccaga
    39241 acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt
    39301 ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgta tatgccaaaa
    39361 acatttcggc agaagggctg caaagcggca agcagttgcc gctcggtccg gcgttattag
    39421 gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct gcccttcccc gatacgacgg
    39481 aaaccggtgc gctgattacc ccgccattta acccgttgca acgtacaccg attgaacatg
    39541 tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcggacgc gggcagcgta
    39601 tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct gctggggatg atggcacgct
    39661 acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag
    39721 attttattga gaatatcctc ggtgccgaag gacgtgcacg ctcagtggtg attgccgctc
    39781 cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc ctatgccacg cgcattgccg
    39841 aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg
    39901 cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acctgccact aaaggttatc
    39961 caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggtaat ggcattagcg
    40021 gcggcggctc gattaccgcg ttttataccg tgctcaccga aggcgatgac cagcaggacc
    40081 ccattgctga ctccgcgcgg gcaatcctcg acggtcacat tgtgctgtct cgccgactgg
    40141 cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagtcgc gcaatgacgg
    40201 cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt
    40261 ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc
    40321 tcgataaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa ggcatttttg
    40381 aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtgtcat
    40441 aacccaggag ataacggcag atggcggaag aacagctcaa aatgttgatc gattatcaga
    40501 atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc aaccgctgga
    40561 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
    40621 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
    40681 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
    40741 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
    40801 aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat tgcctggcgg
    40861 caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat tagcaggcga
    40921 gacaacaact gacaaagcgg ccccccagtt gctggtagca acagataagc ccacgacaaa
    40981 aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt tactgatccc
    41041 tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac cgttaaccac
    41101 cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag acgaaaaagc
    41161 ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga tgttgccggg
    41221 ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg cagagaaacc
    41281 aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg atgacgcccc
    41341 cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
    41401 catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc cacaccagac
    41461 acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt ctcacgaatg
    41521 gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac aaagtgcaga
    41581 gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag tggatgataa
    41641 ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc tggaagcagc
    41701 gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc aaagcaacat
    41761 tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc aaagccaacg
    41821 cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgtctc
    41881 tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac gtcagaggta
    41941 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
    42001 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
    42061 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
    42121 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
    42181 gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt gggcgatgcg
    42241 gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac ccgctcgcgg
    42301 ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc acgtcaggat
    42361 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccaca
    42421 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
    42481 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
    42541 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtatta gcggcgaaag
    42601 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
    42661 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt tcaacctgct
    42721 gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccgtacc atgaatttgc
    42781 ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc tacgcggcac
    42841 agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
    42901 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg aacagcgcgt
    42961 catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga aagcgattaa
    43021 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca atatcaccac
    43081 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
    43141 cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat tgttggttaa
    43201 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
    43261 ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc tacgcctgtc
    43321 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg atcgcatcat
    43381 cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggtactctca acggtcagta
    43441 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
    43501 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
    43561 gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga cgcggtgttc
    43621 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gattatggat
    43681 attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa agagctgttg
    43741 cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc gctggatatt
    43801 ctgatcaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga taaatatggc
    43861 gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag ccgttagtga
    43921 tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca ctgctgcagg
    43981 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
    44041 ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
    44101 cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
    44161 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
    44221 agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt ggcgggagat
    44281 cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta cgcccctcgc
    44341 cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcgggtatc accagccagc
    44401 cgctacctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct
    44461 cgttgacgtt cattccagcg attttactga tgatgaccag tttcacccgc atcattattg
    44521 tttttggttt attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc
    44581 tggggctggc actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg
    44641 tcgatgcgta ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag
    44701 gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt
    44761 ttgccagact ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt
    44821 tgctcccggc ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt
    44881 tcatcccttt tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga
    44941 tgatggttcc ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg
    45001 atggctggca attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa
    45061 tgacacctga atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg
    45121 cagccccgct gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag
    45181 ccacgcagat taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg
    45241 ccatcattat tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt
    45301 tcactaacct gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca
    45361 atggctttcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc
    45421 taccgcgcca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat
    45481 gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    45541 ctttgctctg tggctggccg tacagcagat cctgatcggc attgcgcttg gttttaccat
    45601 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    45661 gtcatttgcg acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat
    45721 catggatatg ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    45781 actgctggtc gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc
    45841 gtttctggca cccaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt
    45901 accgctcatt actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc
    45961 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt
    46021 aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt
    46081 taatttgctg gctgatatta ttagtgaatt accattaata taattccgta acgtttatca
    46141 tgctacccta aggattatcc gaaaaataat atctgcgaac atcttccagg atactcctgt
    46201 agcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca
    46261 ggcaaattat acaacacttt acgggatagt aagtccgcct gaaaaattgc gagagtggcg
    46321 cattaggtga cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta
    46381 tcgttacgca ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac
    46441 acccgactag gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac
    46501 gacattgaaa ccgttgatga ccttgccata gcttgtgatt cacagcgtcc ttcagtggtg
    46561 tttattaatg aggactgttt catccacgat gcttctaaca gtcagcgcat caagcacatc
    46621 attaatcaac atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttc
    46681 gatgaatatc tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa
    46741 tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta
    46801 aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca
    46861 ggtcagggaa ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg
    46921 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
    46981 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
    47041 actggtggtt tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac
    47101 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa
    47161 ccaggtacat tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg
    47221 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
    47281 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga
    47341 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac
    47401 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa
    47461 acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag
    47521 cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca
    47581 tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga
    47641 agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt
    47701 gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc
    47761 ttatctgcaa tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact
    47821 tatgaataga aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc
    47881 ccgaaaaaat catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta
    47941 ctgcgcgacc atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac
    48001 cctgctggac agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca
    48061 cgaagccaat attcccgtca ttctctgtag cagtaaaact ttagcggaaa tgctgtactt
    48121 gcaaaaaatg ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca
    48181 gcttgctgaa caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca
    48241 tggcgaaatc tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac
    48301 ttttgatgat gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc
    48361 ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg
    48421 tatggcacaa tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg
    48481 cttctggcac gtactggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc
    48541 gacctatcaa caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa
    48601 cgatgcgcct ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga
    48661 aggggtacat ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc
    48721 ggaaggctgg cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc
    48781 gaatacccga ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg
    48841 gagttgttca aaatcataat caccggtttc ctcgctgcta cttaccccca gcgaggcact
    48901 gatgcgtatc gttgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat
    48961 acgttctgcg acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc
    49021 ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc
    49081 agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa
    49141 atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc
    49201 gagcggacgg gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg
    49261 ccacgcctgc cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat
    49321 cacataccag gagaggagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat
    49381 gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcgccagcac
    49441 accgtcgaaa tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc
    49501 ccgcgtgtca tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt
    49561 ccctgttgga tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg
    49621 atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac
    49681 gggaatactc atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac
    49741 ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc
    49801 gcggtgacga ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta
    49861 ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccagc
    49921 acgcgagaca tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa
    49981 gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag
    50041 gaaattcccc tcgctgacta atgcatccga tacgccattg actggcaggg tgcgccgtcg
    50101 gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt
    50161 acgcagagag gtgaaatcca gtggcgccac gagagcttcg cgcatgccat tacgcaaaaa
    50221 gatcagtttg tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag
    50281 atgattacgc tagctggaga tataggcatc ttccagcacc accacttcac gccaggtgag
    50341 caaggtggaa aaaagcaata ccacgataaa gcagagatta acgacatgac cgggccccag
    50401 gcggcgtgcg agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct
    50461 gacgactccc aacctgcttt tttatgattc tggcataacc cggcaatagc gtaccgcaaa
    50521 acagcgataa ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt
    50581 gggacaccag gccggatgtg atccggcctg gtgttaatga taccctttca aacttcttcc
    50641 gcagaacgac tcccctcccc cggaacaaga tcggtttcat gaaaagcttc ccgcttgacc
    50701 ccgtaaccgt cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc
    50761 cgcgggccgc cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc
    50821 ttttttcgag tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagccag
    50881 tgcgagtatt aatgtgcctg accgcgcatt ttcgctactg ctttcactcc cagcaccacg
    50941 atgccgccga tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc
    51001 actgcacttt gctgcccggc gatatgttca atagcgtgat gcagcggcgc aataccatgt
    51061 accacaatcc cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taatgctttc
    51121 atcagccaag gcgcgataat caataaccct ttacctaatg cctgcatcag cgcgctagat
    51181 ttttccgcca gccaataccc taggtcatca atcttaacga taacccctac cagaccgtag
    51241 acgcccacgg tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc
    51301 gcctctgcca caatccccag cgtgatggcg acgatttccg cagacaaaat aaaatcggta
    51361 cgaatcgccc ctttaatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc
    51421 tccagacgct gctggctctg cgctggatct tctttatgtt tacgcgcctc cagcatatgc
    51481 agcactttct ctaccccttc aaagcagaga aacgcgccac caatcatcaa cagtggcgta
    51541 atcgcccacg ggataaacgc actgatgatc agcgccagcg gcaccagaat cactttattg
    51601 atcagcgatc ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct
    51661 gaaacttgtt gcgcattgag cgataaatca tcccctaata caccggcggt tttcttcgcc
    51721 gccagtttgc ccatcacaga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc
    51781 agtaaactac tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta
    51841 tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc aacatttatt taacaatcaa
    51901 gtgcaattat atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta
    51961 tttccgccgt gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt
    52021 gcgttgtata tcatttgggg ctcaacctat tttgtcattc ggattggcgt gggaagctgg
    52081 cctccgttaa tgatggcggg cgttcgattc caggcagccg gtattttatt gctggcattt
    52141 ttgctactgc gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt
    52201 ggcctgttat tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt
    52261 ccttccggca tcgccgccgt agtgattgca accgtgcccc tctttaccct gtgcttcagt
    52321 cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg gtattgccat tgggcttgcc
    52381 ggaatcatca tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg
    52441 attttaatcg gctcgattag ctgggcgttt ggctcagttt atggctcgcg cattacctta
    52501 cctgtaggga tgatggcggg tgcgattgag atgctggcgg caggcgtggt gttaatgatc
    52561 gcatcgatga ttgcgggtga aaaactgacg gcgctccctt ccctgtcagg cttccttgcg
    52621 gtcggctatc tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc
    52681 cgtaatgtca gtccggctct cgccaccagc tacgcctacg ttaacccggt ggtcgcggtc
    52741 ttgctgggta cgggactggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc
    52801 gtaattgttt tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaaaccc
    52861 gttgtcgcgc cagttattca ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat
    52921 ctgcgcgctg gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc
    52981 cgtcagcttc tcgcgcctac gtaacgcaca ctcccagaca atcagtacgc gccagccgag
    53041 ctcctgcaag cgactgatat cgcggcgatc gcgttcaaca tttttaccta tcttctccag
    53101 ccagaactca gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa
    53161 gcagccgtgg gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg
    53221 cagactggcg tcctgaacgc gaaacgcaag gccctgcccg gttaacaaac tggcgaggcg
    53281 cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt
    53341 atcgtgaacg tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt
    53401 ttggctcaag cagttttgcc accgcggcaa agaccggcac gaccaccgag ttgccgaact
    53461 ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa
    53521 aacccattaa gcgcgcgcat tcacgaggcg ttaaccgacg tggacgatgt tgctgattca
    53581 gcggatcgtc aaagtctttc tcacccgtgg ccatatccca accgcgatcg attaaaattt
    53641 ccgcgccatc tttgtaataa cgagcagaca gcgtacgggt gacgctttgc ggattgttcg
    53701 gataaaccat tccataaccg aagccgttac cgcgcgcctg atgttttttc gcatatcgat
    53761 agaggtactt ccacagcacc ggcgtcagga tatatttcgc ctcaaccatc gggtccagca
    53821 gctgcgccag cgtcactcgc tgtgcaggga aacattcgct gatatcacgc agagtaaaat
    53881 cggctttcag attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg
    53941 gcaggaaatg tttaccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcgg
    54001 caacgtcata gcccagttcg tccagcgtct gcatgatgat gcggaacgtt tttcccttat
    54061 cgtgactctt caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga
    54121 taatgcgcac cacatcaaag aacagcgtgc cctgggtatc gcaggcaaaa ccgtgcgccc
    54181 gcccgagcga gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg
    54241 ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac
    54301 tcacgccttc ttgatgactg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg
    54361 ccgggtcgca ataatggttg gctttataag tgcgtaccgc atgtttgttc cattcgctgg
    54421 taaacacgca ctgtccacca atcgattcaa aaccacgacg gatgccgcca attccggcaa
    54481 atagatcgat aaagcgaaac gcataatgcg gatgatgttc cggtggtttg ggtaacaacg
    54541 tttgcagatg ggcaaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg
    54601 cacgttttaa aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca
    54661 gcattttaac atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg
    54721 tgctgtatga atcggttact gatatatttt cctgcataga tttaaccggc catctgagat
    54781 ggccgacagg ttaacataat tactgccgtt tagggccacg cgctcagcca gcgggtgaaa
    54841 acgcatctat caccttgtga tcgatacctt cattctcccc cgccagttcg gcaccgagct
    54901 tcgccatcaa acagcgccaa gctcaatgcc cctgaaacgg caaatacgcg cgccaggcca
    54961 atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag
    55021 ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcaattttc
    55081 tccgccggaa attgcacgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg
    55141 attgaagaac gctgccgttg cggatgattt ttcgccaggc tcacgatatc gtgaaaataa
    55201 caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagttt ctgtgccgta
    55261 gcccagacac ggcgaaaatg acacaggtca tgcgccgcgt cctgatgttg atgatggttt
    55321 ttcaaccagt tctcgaacta tgcctgccag tgttgtaact ccatacgcgc tccgttgcca
    55381 aagagtctca accttagcag tttttattca tagcgtctgc aacgtgcgga acgaggtcgg
    55441 gtgtaccatg caatgccgcc taacaaggca ccaaccaggg ccataaacag aaatccgacc
    55501 aatgctccta accacttccc cgcggtagat cctaaatcac tttcaaatcc cgacacgggg
    55561 gcatcgggga tccggctcat cacccagatg tatcgcgcca ttgcccagac gaaatagcca
    55621 caccatgtat agaaagccc
//
LOCUS       DAJTRW010000016        55409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRW010000016 DAJTRW010000000
VERSION     DAJTRW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(104..1477)
                     /gene="argH"
                     /locus_tag="OO326_001057"
     CDS             complement(104..1477)
                     /gene="argH"
                     /locus_tag="OO326_001057"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5965523.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1538..2314)
                     /gene="argB"
                     /locus_tag="OO326_001058"
     CDS             complement(1538..2314)
                     /gene="argB"
                     /locus_tag="OO326_001058"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5965524.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2322..3326)
                     /gene="argC"
                     /locus_tag="OO326_001059"
     CDS             complement(2322..3326)
                     /gene="argC"
                     /locus_tag="OO326_001059"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5965525.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3480..4631
                     /gene="argE"
                     /locus_tag="OO326_001060"
     CDS             3480..4631
                     /gene="argE"
                     /locus_tag="OO326_001060"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5965526.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4983..7634
                     /gene="ppc"
                     /locus_tag="OO326_001061"
     CDS             4983..7634
                     /gene="ppc"
                     /locus_tag="OO326_001061"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5965527.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7817..9550
                     /locus_tag="OO326_001062"
     CDS             7817..9550
                     /locus_tag="OO326_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5965528.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9699..10550
                     /locus_tag="OO326_001063"
     CDS             9699..10550
                     /locus_tag="OO326_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5965529.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10537..10878)
                     /locus_tag="OO326_001064"
     CDS             complement(10537..10878)
                     /locus_tag="OO326_001064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5965530.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10880..11758)
                     /locus_tag="OO326_001065"
     CDS             complement(10880..11758)
                     /locus_tag="OO326_001065"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5965531.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11724..14021)
                     /locus_tag="OO326_001066"
     CDS             complement(11724..14021)
                     /locus_tag="OO326_001066"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5965532.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14072..14392)
                     /locus_tag="OO326_001067"
     CDS             complement(14072..14392)
                     /locus_tag="OO326_001067"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5965533.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14407..15486)
                     /locus_tag="OO326_001068"
     CDS             complement(14407..15486)
                     /locus_tag="OO326_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5965534.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15795..16898
                     /locus_tag="OO326_001069"
     CDS             15795..16898
                     /locus_tag="OO326_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5965535.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16899..18296
                     /gene="ptsP"
                     /locus_tag="OO326_001070"
     CDS             16899..18296
                     /gene="ptsP"
                     /locus_tag="OO326_001070"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5965536.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18308..18970
                     /gene="fsa"
                     /locus_tag="OO326_001071"
                     /pseudo
     CDS             18308..18970
                     /gene="fsa"
                     /locus_tag="OO326_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18981..20082
                     /gene="gldA"
                     /locus_tag="OO326_001072"
                     /pseudo
     CDS             18981..20082
                     /gene="gldA"
                     /locus_tag="OO326_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20964
                     /locus_tag="OO326_001073"
                     /pseudo
     CDS             20357..20964
                     /locus_tag="OO326_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20991..21896)
                     /gene="yijE"
                     /locus_tag="OO326_001074"
     CDS             complement(20991..21896)
                     /gene="yijE"
                     /locus_tag="OO326_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5965537.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21990..24170)
                     /gene="katG"
                     /locus_tag="OO326_001075"
     CDS             complement(21990..24170)
                     /gene="katG"
                     /locus_tag="OO326_001075"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5965538.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24499..25389)
                     /gene="metF"
                     /locus_tag="OO326_001076"
     CDS             complement(24499..25389)
                     /gene="metF"
                     /locus_tag="OO326_001076"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5965539.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25738..28170)
                     /gene="metL"
                     /locus_tag="OO326_001077"
     CDS             complement(25738..28170)
                     /gene="metL"
                     /locus_tag="OO326_001077"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5965540.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28173..29333)
                     /gene="metB"
                     /locus_tag="OO326_001078"
     CDS             complement(28173..29333)
                     /gene="metB"
                     /locus_tag="OO326_001078"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5965541.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29610..29927
                     /gene="metJ"
                     /locus_tag="OO326_001079"
     CDS             29610..29927
                     /gene="metJ"
                     /locus_tag="OO326_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5965542.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30111..30719
                     /locus_tag="OO326_001080"
     CDS             30111..30719
                     /locus_tag="OO326_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5965543.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30780..30992)
                     /gene="rpmE"
                     /locus_tag="OO326_001081"
     CDS             complement(30780..30992)
                     /gene="rpmE"
                     /locus_tag="OO326_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5965544.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31195..33393
                     /gene="priA"
                     /locus_tag="OO326_001082"
     CDS             31195..33393
                     /gene="priA"
                     /locus_tag="OO326_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5965545.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33549..34574
                     /gene="cytR"
                     /locus_tag="OO326_001083"
     CDS             33549..34574
                     /gene="cytR"
                     /locus_tag="OO326_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5965546.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34666..35625
                     /gene="ftsN"
                     /locus_tag="OO326_001084"
     CDS             34666..35625
                     /gene="ftsN"
                     /locus_tag="OO326_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5965547.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35718..36248
                     /gene="hslV"
                     /locus_tag="OO326_001085"
     CDS             35718..36248
                     /gene="hslV"
                     /locus_tag="OO326_001085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5965548.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36258..37589
                     /gene="hslU"
                     /locus_tag="OO326_001086"
     CDS             36258..37589
                     /gene="hslU"
                     /locus_tag="OO326_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5965549.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37656..38582
                     /gene="menA"
                     /locus_tag="OO326_001087"
     CDS             37656..38582
                     /gene="menA"
                     /locus_tag="OO326_001087"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5965550.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38675..39160
                     /gene="rraA"
                     /locus_tag="OO326_001088"
     CDS             38675..39160
                     /gene="rraA"
                     /locus_tag="OO326_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5965551.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39245..39490)
                     /gene="zapB"
                     /locus_tag="OO326_001089"
     CDS             complement(39245..39490)
                     /gene="zapB"
                     /locus_tag="OO326_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5965552.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39916..40761
                     /gene="glpF"
                     /locus_tag="OO326_001090"
                     /pseudo
     CDS             39916..40761
                     /gene="glpF"
                     /locus_tag="OO326_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40801..42291
                     /gene="glpK"
                     /locus_tag="OO326_001091"
     CDS             40801..42291
                     /gene="glpK"
                     /locus_tag="OO326_001091"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5965553.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42521..43531
                     /gene="glpX"
                     /locus_tag="OO326_001092"
     CDS             42521..43531
                     /gene="glpX"
                     /locus_tag="OO326_001092"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5965554.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43628..44374
                     /gene="fpr"
                     /locus_tag="OO326_001093"
     CDS             43628..44374
                     /gene="fpr"
                     /locus_tag="OO326_001093"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5965555.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44379..44807)
                     /gene="uspD"
                     /locus_tag="OO326_001094"
     CDS             complement(44379..44807)
                     /gene="uspD"
                     /locus_tag="OO326_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5965556.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44834..45133)
                     /locus_tag="OO326_001095"
     CDS             complement(44834..45133)
                     /locus_tag="OO326_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5965557.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45345..45785)
                     /locus_tag="OO326_001096"
                     /pseudo
     CDS             complement(45345..45785)
                     /locus_tag="OO326_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45886..46485
                     /locus_tag="OO326_001097"
     CDS             45886..46485
                     /locus_tag="OO326_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5965558.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46593..47360
                     /gene="tpiA"
                     /locus_tag="OO326_001098"
     CDS             46593..47360
                     /gene="tpiA"
                     /locus_tag="OO326_001098"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5965559.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47415..48170)
                     /gene="cdh"
                     /locus_tag="OO326_001099"
     CDS             complement(47415..48170)
                     /gene="cdh"
                     /locus_tag="OO326_001099"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5965560.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48277..49266)
                     /gene="sbp"
                     /locus_tag="OO326_001100"
     CDS             complement(48277..49266)
                     /gene="sbp"
                     /locus_tag="OO326_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5965561.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49586..50548)
                     /gene="pfkA"
                     /locus_tag="OO326_001101"
     CDS             complement(49586..50548)
                     /gene="pfkA"
                     /locus_tag="OO326_001101"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5965562.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50729..51631)
                     /gene="fieF"
                     /locus_tag="OO326_001102"
     CDS             complement(50729..51631)
                     /gene="fieF"
                     /locus_tag="OO326_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5965563.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51779..52279)
                     /gene="cpxP"
                     /locus_tag="OO326_001103"
     CDS             complement(51779..52279)
                     /gene="cpxP"
                     /locus_tag="OO326_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5965564.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52429..53127
                     /gene="cpxR"
                     /locus_tag="OO326_001104"
     CDS             52429..53127
                     /gene="cpxR"
                     /locus_tag="OO326_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5965565.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53124..54497
                     /gene="cpxA"
                     /locus_tag="OO326_001105"
     CDS             53124..54497
                     /gene="cpxA"
                     /locus_tag="OO326_001105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5965566.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54603..55277)
                     /gene="yiiM"
                     /locus_tag="OO326_001106"
     CDS             complement(54603..55277)
                     /gene="yiiM"
                     /locus_tag="OO326_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5965567.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13491 a        15016 c        14160 g        12742 t
ORIGIN      
        1 caaatttctg cctgatgcgg cgtaaacgcc ttatccgcct acgaagtgtc gcactttgta
       61 ggcgtggtaa actccccatt ttcaggggag caatataaac aaattactct aaccgagcct
      121 gcgcaaaagc aatcgcctgc gccacctgct gcggtgagac gccgcctttt gccgcacgct
      181 tttcgaggca cgattgtagc gacagaatcg gatagacatc ttcgccaatc acctgactga
      241 atttctgcaa ctcactgagt ggcagatctt ccagcggttt gccctgacga atggcttcca
      301 ccaccgcttc acccacaata tggtgcgcct cgcggaaagg tacgcctttc gccaccaggt
      361 aatccgccag ttcagtggcg ttcgcatacc cctgctgcgc cgcttcctga caacgcggac
      421 gtttcacctg aatgccgtcc agtaccagcg ccgccatatg caggcagtcc agccaggtat
      481 cgagcgcgtc gaacagacct tctttgtctt cctgcatatc tttgttgtaa gccagcggca
      541 aacctttcag cgtcatcatc atgccggtta acgcaccctg cacccggccg catttaccgc
      601 gaatcagctc cagcgcatcc gggtttttct tctgcggcat taatgatgaa ccggaagtca
      661 cgcggtccga aagctccaca aaccccgctt cgccggtgtt aaagaaaatc agatcttcag
      721 caaaacgcga cagatgcacc atgccgatag cggcagcaga aagcagttcc aacacatggt
      781 cacggtcaga aacgctgtcc agactgttac gggtcgccga agcaaagccc agccagcctg
      841 ctaactgttc acggtcgatt tcataggccg ttcccgccag cgcgccacag cctaatgggc
      901 tgacatccag acgcttaagc gcatcctgca aacggctttc atcacgcgcc agcatctcaa
      961 cataggccag gcaccagtgc gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac
     1021 ctggcattac cgcgtcctga ttgttttgtg cggtttccac cagcgccgat tgcagctgcc
     1081 ggttagccgt cagtaactcg ctaacggtat ctttgcacca cagtttcagg tcagtcgcta
     1141 cctgatcatt acggctacgc ccggtatgca gctttttgcc taactggccc actttgtcga
     1201 tcagtttgcc ttccacccag ctatggatat cttcggcgtc gctttcaagg atttgttgtg
     1261 gcctggcgcg aacatcttcc agcaacacgt tcagcgcctc ttccagttgc gcctgctctt
     1321 ctgcggttaa cacgccgacc gttaccaagg ctttggacca ggccacagag ccaacaatat
     1381 cctgctccgc cagacggtaa tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat
     1441 ctgctgcctg ggtaaaacgc ccgccccaaa gtgccataac tctgtttcct tattttgaaa
     1501 ttcaatgccg gaaagctgcg cctccggcta acaaaactta agctaaaatc cgcgtaccca
     1561 tcggcatacc gttaaacagt gccggaagct gctccgcata acgccaagaa gcgatatcta
     1621 ccggacggcc cagcgtgcgg gccgcatcca gcgccgcgtt cactttcact atcatgccgt
     1681 cagtaataat gccctgctca atcagttgtt ctgctttcgc ggcggtcatt tcggcaatgc
     1741 gttgcccttt gccgtcgaga atgccgctga cgtcggagag caaaatcaga tccgcgccca
     1801 gcgttgccgc cagcgccgtt gccgcctggt cggcattgac gttcatcagt tgcccttcgt
     1861 ctgttacgcc aatggagctg accaccggca gataaccgtt ctccagcaag gagttgataa
     1921 gcttaggcga acctggctgc gccagtccaa catgacctaa ctcttcatca agctgggtca
     1981 ctttgacgct gtcgccgtca ccgagaaaca aacctacggc cgcaatctga tgtttcttcg
     2041 cccatgccaa cagggtttta tttgccgttc ccgccagtgc tccggtgata atgtctatct
     2101 gatcagcagg cgtcacccgc aggccgtttt tctttttcac cggcagattc agccctttca
     2161 tcagctcatc caccacgcaa ccgccgccgt gcacaatcac cagcggacgc tgatgtgact
     2221 cacgataatt caccaatgcg ctaaacagac gttccagcgc ctcttcacta tccagcagta
     2281 cgccgcccag tttgataatt aatggattca tcattgcacc cttaaataag agactgcgtt
     2341 tccgcatagc cgaaacgaat attggcgcac tgtaccgctt gtgccgccgc gcctttcagt
     2401 aagttgtctt cggtcgccac aataatcaga tgctcgccct gaacggcaaa cccgatatcg
     2461 caaaatggca gcccaacgac atttttcagc gccggaacgc ctttgtcata cagccgcacc
     2521 agcggtttat gggcatacgc ctgctgtaac gcttgcgcga cttgcgcctg ggtcacaccc
     2581 gatttcaggc ggcaggtaat ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg
     2641 gtgaagataa cgtcagcacc gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag
     2701 acgccatacg gttgcaggct aacttcacaa aaactatttg aaatcgctgc cttacgtcct
     2761 gcaccgctca cgccgctggt ggcgttgatc accggccact gattgaggtc aagaagatcg
     2821 gcatcaatca acggtttcag cgccagctgt gccgccgtcg gataacagcc tggcaccgca
     2881 atcaaattcg cttcttttaa tttattaccg caccactccg ccagaccgta ggctgcctgt
     2941 tccagcagtt ccgggtattg atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg
     3001 ttaacacgaa acgcgccgga aaggtcgaac accacgcagc ccgcttcaag aaactgcggc
     3061 gctaaatcgt ggctaacttc atgggcggtg gcgagaaaca ctacgtccac ccctgggcta
     3121 aactcgctga tatccgacat cggctgcaac ggcagctcaa cgatgccttt tagctgcgga
     3181 tgcaaatcgg agattaactt tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt
     3241 atgttcatat gcggatggcg atttacatag gtcactagct ctgcgccagc gtagccgctg
     3301 gcacccacaa tcagcgtatt caacatcggg gctattcacc ttcttatgtc tggttgccag
     3361 gttaaacgta aaacattcac cttacggctg gtgggtttta ttacgctcaa cgttagtgta
     3421 tttttattca taaatactgc atgaatattg atactatcat gaccagaggt gtgtcaacaa
     3481 tgaaaaacaa attaccgcca tttatcgaga tttaccgcgc tctgattgcc acaccttcaa
     3541 taagcgctac ggaagaggca ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg
     3601 actggtttaa agatttgggc ttcaatgtgg aagtgcagcc tgttccagga actcgcaaca
     3661 aatttaatat gctggccagt tacggacagg gggctggcgg cttgttgctg gcggggcata
     3721 ccgatacggt gccatttgat gacggtcgct ggacgcgcga tccgtttaca ctgacggagc
     3781 atgacggcaa gctttacggc ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc
     3841 ttgatgcgct acgcgatgtc gacgtcacga aactgaaaaa accgctctac attctggcga
     3901 ctgctgatga agaaaccagt atggccggag cgcgttattt tgccgaaact accgccctgc
     3961 gcccggattg cgccatcatt ggcgaaccga cgtcactaca accggtacgc gcacataaag
     4021 gtcatatctc taacgccatc cgtattcagg gccagtcggg gcactccagc gatccagcac
     4081 gcggaattaa cgctatcgaa ctaatgcacg acgccatcgg gcatattttg caattgcgcg
     4141 ataacctgaa agaacgttat cactacgaag catttaccgt gccataccct acgctcaacc
     4201 tcgggcatat tcacggtggc gacgcttcta accgtatttg cgcttgctgt gagttgcata
     4261 tggatattcg tccgcagcct ggcatgacac tcaatgaact taatggtttg ctcaacgatg
     4321 cattggctcc ggtgagcgaa cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc
     4381 cgatccctgg ctatgaatgc ccaccgaatc atcaactggt tgaagtggtt gagaaattgc
     4441 tcggagcaaa aaccgaagtg gtgaattact gtaccgaagc gccgtttatt caaacgttat
     4501 gcccgacgct ggtgctcggt ccgggttcca tcaatcaagc tcatcagcct gatgaatatc
     4561 tggagacgcg gtttattaaa cccacccgtg agttgattat ccaggtaatt caccattttt
     4621 gctggcatta aaacgtaggc cggataaggc gctcgcgccg catccggcac cgttgccaaa
     4681 ctccggtgcc gcaataatgt cggatgcgat gcttgcgcat cttatccgac ctacaccttt
     4741 ggtgttatgt atggcgattt tttaacattt ccataagtta cgcttattta aagcgtcgtg
     4801 aatttaatgg cgtaaattcc tgctatttat tcgtttgctg aagcgatttc gcagcatttg
     4861 acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc gagcatattc
     4921 gcgccaatgc gacgtgaagg atacagggct atcaaacgat aagatggggt gtctggggta
     4981 atatgaacga acaatattcc gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg
     5041 gagaaaccat caaggatgcg ttgggagaac acattcttga acgcgtagaa actatccgta
     5101 agttgtcgaa atcttcacgc gctggcaatg atgctaaccg ccaggagttg ctcaccacct
     5161 tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga
     5221 acctggccaa caccgccgag caataccaca gcatttcgcc gaaaggcgaa gctgccagca
     5281 acccggaagt gatcgcccgc accctgcgta aactgaaaaa ccagccggaa ctgagcgaag
     5341 acaccatcaa aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg gctcacccaa
     5401 ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc tgtttaaaac
     5461 agctcgataa caaagatatc gctgactacg aacgcaacca gctgatgcgt cgcctgcgcc
     5521 agttgatcgc ccagtcatgg cataccgatg aaatccgtaa gctgcgtcca agcccggtag
     5581 atgaagccaa atggggcttt gccgtagtag aaaacagcct gtggcaaggc gtaccgaatt
     5641 acctgcgcga actgaacgaa caactggaag agaatctcgg ctacaaactg cccgtcgaat
     5701 ttgttccggt acgttttacc tcgtggatgg gcggtgaccg cgacggcaac ccgaacgtca
     5761 ctgccgatat cacccgccac gtcctgctgc tcagccgctg gaaagccacc gatctgttcc
     5821 tgaaagatat tcaggttctg gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc
     5881 tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc
     5941 gttctcgcct gatggcgaca caggcatggc tggaagcgcg cctgaaaggc gaagaactgc
     6001 caaaaccaga agggctgctg acacaaaacg aagaactgtg ggaaccgctc tacgcttgct
     6061 accagtcact tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc
     6121 tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt caggagagca
     6181 cccgccatac cgaagcgctg ggcgagctga cccgctacct cggtatcggc gactacgaaa
     6241 gctggtcaga ggccgacaaa caggcgttcc tgatccgcga actgaactcc aaacgtccgc
     6301 ttctgccgcg caactggcaa ccaagcgccg aaacgcgcga agtgctcgat acctgccagg
     6361 tgattgccga agcgccgcaa ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc
     6421 cgtccgacgt actggctgtc cacctgctgc tgaaagaagc gggtatcggg tttgcgatgc
     6481 cggttgctcc gctgtttgaa accctcgatg acctgaacaa cgccaacgat gtcatgaccc
     6541 agctgctcaa tatcgactgg tatcgcggcc tgattcaggg caaacagatg gtgatgattg
     6601 gctattccga ctcagcaaaa gatgcgggcg tgatggcagc ttcctgggcg caatatcagg
     6661 cacaggatgc attaatcaaa acctgcgaaa aagcgggtat tgagctgacg ttgttccacg
     6721 gtcgcggcgg ttccattggt cgcggcggcg cacctgccca tgcggcgctg ctgtcacaac
     6781 cgccaggaag cctgaaaggt ggcctgcgcg tgactgaaca gggcgagatg atccgcttta
     6841 aatatggtct gccagaaatc accgtcagca gcctgtcgct ttacaccggg gcgattctgg
     6901 aagccaacct gctgccacca cctgagccga aagagagctg gcgtcgcatt atggatgaac
     6961 tgtcagtcat ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc
     7021 cttacttccg ctccgctacg ccggaacaag aattgggcaa actgccgttg ggttcacgtc
     7081 cggcgaaacg tcgcccaacc ggcggcgtcg agtcgctgcg cgccataccg tggatttttg
     7141 cctggacgca aaaccgcctg atgctccccg cctggctggg tgcaggtacg gcgctgcaaa
     7201 aagtggtcga agatggcaaa cagagcgagc tggaagccat gtgccgcgat tggccattct
     7261 tctcgacgcg tctcggcatg ctggagatgg tcttcgccaa agcagacctg tggttggcgg
     7321 aatactatga tcaacgtctg gtagacaaag cactgtggcc gttaggtaaa gagttacgca
     7381 acctgcaaga agaagacatc aaagtggtgc tggcgattgc caacgattcc catctgatgg
     7441 ccgatctgcc gtggattgca gagtctattc agctacggaa tatttacacc gacccgctga
     7501 acgtattgca ggccgagttg ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac
     7561 cggatcctcg cgtcgaacaa gcgttaatgg tcactattgc cgggattgcg gcaggtatgc
     7621 gtaataccgg ctaatcttcc tcttctgcaa accctcgtgc ttttgcgcga gggttttctg
     7681 aaatacttct gttctaacac cctcgttttc aatatatttc tgtctgcatt ttattcaaat
     7741 tctgaatata ccttcagata tccttaagga attgtcgtta tattcggcga tatttttttc
     7801 aagacaggtt cttactatgc attccacaga agtccaggct aaaccccttt ctagctggaa
     7861 agccctaggt tgggcactgc tctacttttg gtttttctct actctgctac aggccattat
     7921 ttacatcagt ggttatagtg gcaccaacgg cattcgcgac tcgctgttat tcagctcgct
     7981 gtggttgatc ccggtattcc tctttccgaa gcggattaaa attattgccg cagtgatcgg
     8041 cgtggtgcta tggacggcct ctctggcggc gctgtgctac tacgtcatct acgggcagga
     8101 gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta
     8161 tttaagccag tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcggtggc
     8221 agttctgctg tggacacgcc tgcgcccggt ctatattcca aagccgtggc gttatgttgt
     8281 ctcttttgcc ctgctttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa
     8341 aaacaagccg tttgagaaaa cgttggataa cctagcctcg cgtatggagc ctgccgcacc
     8401 gtggcaattc ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagtt
     8461 actgaatgaa aataatgcct tgccgccact ggctaatttc aaagatgaat cgggtaacga
     8521 accgcgcact ttagtgctgg tgattggcga gtcgacccag cgcggacgca tgagtctgta
     8581 cggttatccg cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct
     8641 gaccgtgttt aataacgtag ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc
     8701 gctgaccttt gccaatgaaa agaacccgga tctgtatctg acgcagccgt cgctgatgaa
     8761 catgatgaaa caggcgggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc
     8821 ccgcaatacc atgctgacgg tattttcgcg ccagaccgac aagcagtact acatgaacca
     8881 gcaacgtacg cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt tccaggatgt
     8941 gctgaatgac cctgcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa
     9001 atacaaatac cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc
     9061 gccaggatta agcgcagaag agctggaatc atataacgat tatgacaacg ctaacctgta
     9121 taacgatcat gtggttgcca gcctgattaa agactttaaa gcggcagacc cgaacggatt
     9181 cctggtttac ttctctgacc acggtgaaga ggtttacgac acgccgccgc acaaaactca
     9241 ggggcgtaac gaagacaacc cgacgcgcca catgtacacc attccgttcc tgctgtggac
     9301 gtcggaaaaa tggcaagcga ctcatccccg tgatttctca caggatgtcg atcgtaaata
     9361 cagcctggcg gaactgatcc acacctggtc agatttggcg ggcttatctt acgacggtta
     9421 cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa
     9481 cccgtacaag aaaaacgcgc tgatcgatta cgacactctg ccgtatggcg atcaggtggg
     9541 taatcagtag atgacctttg tatcgcgtct ggcgctattt gccggatgcg atactgccgc
     9601 aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt taataatgcc
     9661 agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac gtcagctatc
     9721 tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt gccagcagta
     9781 acggcccggt gcccgatctt gcgtatcagg tcgattttcc acggctggaa attgtgctgg
     9841 aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat gtgctgtacg
     9901 ttgccgctgg tggctggaat ttcccacaat ggaaaacacc cgctaccacc tttagcgtgc
     9961 tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa caatatcaaa
    10021 atctggcgaa gcaacacgtc gcccgacgcg gcccacgcat aggttctttt ctgctacaaa
    10081 cgctcaatga aatgcaaatg cagccgcagg agcagcaaac ggcaaggctt atcgtagcca
    10141 gcctgcttag ccactgccgc gatttgctcg gcagccaaat ccagaccgcc tcacgcagcc
    10201 aggcactatt tgaagctatt cgcgattata tcgacgaacg ctatgcctcc gcgcttaccc
    10261 gcgaatctgt cgcacaggca ttctatattt caccaaacta tctctcgcac ctgtttcaaa
    10321 aaacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag cacgctaaga
    10381 cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg
    10441 acagcaacta cttctgccgt ctgtttcgta aaaacaccga acactcgccg tcggaatatc
    10501 gccggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa gatgggtttg
    10561 ctgcggcgca gaaagcactt tgcgcaccgc gctcattacc cgctgcggct cacgcaggaa
    10621 tgcgtagatg ctgcattgca catagcggca atgttcaaat cgctcggcac cggcaagctc
    10681 gatatccgta atcaacagag caacatccgc ccgacggata tcctcgtccg ctaaacgatt
    10741 ctccgttccc agcgcgccct gagtttcaat gctgacgccc cacttctcta actggcacaa
    10801 cttttccagc cgttccgccg ccatataagt atgcgccacg ccgcttacgc aggcggttac
    10861 tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc cgttctgcca
    10921 tttcgcgcat cgttgtcaca tcggctgacg acggcgcagg cacctctttc atcgaccatg
    10981 ttttccccag caggcggtat ttcggttcgc cgtactgatg aaacggtaac agatggatct
    11041 gcctgatttt cagcgggatc agtacatcca gcgcctgctg catattctcc cggctgagcg
    11101 tgaaaccagg gatcaacggt aaacgcggga tcacgttgac accctcactc accagcaaac
    11161 gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt
    11221 ccataatttt taaatcgaac agcacttcat cgcacaattt cgccagcggt aacagcttgc
    11281 ttgctggcgc gtctccggca gtttcaatgg cgcatgagac accccacagc cgcagtcgct
    11341 gtaaaaaacg ggtagcaaac tccgcctgca ttaacacttc gccgccagaa agcgtgacgc
    11401 cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc agagcgtcaa
    11461 ggctgatatc gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc gcatcacgca
    11521 aacatttcgc acagtgcaga catttcgcct ctctgcgtac cgtctggatt ttgccggaga
    11581 tcgactccgg attagcacac cacgggcaaa gatgcggaca gcctttaaaa aagaccaccg
    11641 tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat
    11701 catcgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat gatgtcatcc
    11761 tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg caccaccagc
    11821 ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta
    11881 aactgaatat gctgcaactt aagctgggta aacacccgta agaagtcggc cagtttgcgt
    11941 aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta
    12001 gacagcagcg tattatcgag cttactgact gacttcagta ccgccgttgg cccttgtgcg
    12061 tcctgaccca gcataggtga caagccgccg tctgccagtt gttccccggc aaaacgaccg
    12121 tctggcgtcg cgccaaccac cgatcccaac ggaacgtgag cagaaacggt atacgatccc
    12181 ggcgtgaagt aaccgccgcg cgggttctgg tatttttcca cttctttgca gtagtggcgc
    12241 aacagttcgg cgctaatgtt atccacctcg tcgatatcgt taccgtattt ctcaaagcgg
    12301 ttaattaagc gagcgcggac tttttcgcct tctggcgttg caaagttggc ttttaatacc
    12361 gacagcaatt cgtcaaaact taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg
    12421 agagaatcgc tcaggttggc gataccaatt ccctgtacgc cggagaagtt ataacgcgcg
    12481 ccgccatcgg taatatcgcg acctttttcc agacaatcgc tgataaaaga cgagagcagc
    12541 ggtacaggtg cccagtcgcg atggccgata tcacaaatat tgctgccctc aaccatcagg
    12601 gtgatgtagt ggctgatctt ggcgcggatc tgctccagta aaccttcata agtcagcgca
    12661 gcattgcctt cattttcatg caggcagatt tccatcactt tcagcagatt aaacatcgcg
    12721 atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc cactacggaa
    12781 tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggcac caccacttca
    12841 tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttcggcggt tttcatcagg
    12901 aacggcgtgt cgatcaaggc gttagtgcgc acgccgaggt tcggttgcgg taattgcacg
    12961 ctttgatagg cgtcaaggca aaggaacgaa agcacgttca ccgcgctacg tccgttctcg
    13021 gttaacccgc cgagcagtgc ggtatagccg gtcgggaaac cggcgaaata acgcgcgcta
    13081 ctggtggagc gcaacagcac gatgtcgttg catttcaccc ataaagattc gagcagttct
    13141 ttcaggaacg ccggatcttc gccctgggtt aaagatgcct gatagaacgg caacatatac
    13201 tggtcgaagc gccctaaaga cagcgaactg gcgttggatt cgtattgcag aatgatgttc
    13261 atgtaccaga ataactggca cgcctgccag aacgtctgcg gcttatgttg cgcgttatgg
    13321 cgggagatct ccgcaatagt cagcagctct tcgcgacgct gggcatctgt gcagtttgcc
    13381 gccatcgttt ccgccagttc ggcgtaacgc aaaatgtgtt tctgcgaggc ttccagcagt
    13441 aacaacgcgg cctgataaaa gtgattctcc ggctgttgct gacaatgttg ctgcatctgt
    13501 gccaccagct cacccagacc gtgattcaac aggcgtgggt aatcaataat aatgtgcccc
    13561 tggcctttat ccgtctggtt gatgctgaaa atctgcgtgt tggtcgcggc ttttacttca
    13621 tctgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta cgggaacaac
    13681 tcttcacggt agatacgttt atcttcttcg ctgatagcaa agcggtcctg cggacgcgtc
    13741 gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga cataatcccg
    13801 gcgcgcggtt ttacggtgcg gttaccggca atcagttctt catcacgaat cgaaatttca
    13861 acatgttcaa ggatatacgc tgttgctttc gcccggcgca atatcaccgg ttcgccttcg
    13921 gtttgccgat ggctggcggt ataaagcagc gcccgctcca gcgagatttc acgggtattg
    13981 gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc ctgtgggaaa
    14041 gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa
    14101 ttttgctcat aatggcgtca gcacgtttta ctgcatcgct gatgttgacg cgcacgatgg
    14161 tcttgccagc aaaacgctct tcaaacttga tgccgatatc tttggtcaga atcaccatat
    14221 cagcgctcgc cacgtcttcc gcagtcagtt cattttccag accaattgac ccctgggttt
    14281 ctactttcac ttcccagcct ttcgctttcg cggcactttc cagcgcctct gctgccatat
    14341 aggtatgggc aacacctgaa ggacatgcgg ttactgcaat aattttcgtc atggctcgtt
    14401 cctcaattaa ttaatttcaa aatccaaatc caggtcgtct tctttttcat cagtcgaaga
    14461 cccattttta cgcgccagac ttttcagcac gttcacacaa acagccgtca ccactgctcc
    14521 gacagccact gctgcgatat aacccaactt accttcaacc accggcagca caatcagacc
    14581 accccagcct gcatagcact gcgcaccgac cagcgccgca gttactgcac cacaaactga
    14641 accgaccata atggaaggaa tgacacgcag cggatcggca gcggcgaaag gaatcgcccc
    14701 ttcagtaacg ccaacacagc ccatcaccag tgccgctttg ccggtttcgc gctcttctgc
    14761 ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg ggatacaaat
    14821 acccacggca gcaatggcca ccacggtata aacaccctga gcaacgcaaa tcagcatgaa
    14881 ggcataggcc actttgttaa ccggaccgcc catatcgaac gccagcatca gccccatgat
    14941 caccgccagc ataacaatgc tgccctgctg catcccctga agccactgag tcaggctgtt
    15001 ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt
    15061 gccgatgata ggaataatga agatgggcat caccgagcgc agaactttat gcaccggaat
    15121 tttcttcagg taatgcacca cgatgccgcc gataatcccg gcgatcagcg caccaaagaa
    15181 gcccgcacca aagctgttac caacccaggc accgatagcg cacggagcca gcgcagaacg
    15241 ctctgcaatg gagtaaccaa tgtaagcggc gaggaaaggc accatcagcg tcaaacctgc
    15301 aacgccgata tcaaacagtt ttttcaaatt cggatcggct acggcatccg gcactgcgcc
    15361 tttgccatac aacatgacgg aaaccgccag caaaataccg cccgatacca cgaagggaat
    15421 catgtgtgaa acgcccgtca ttaaatgctg acgggtattt ttcaggatct gcaccaactc
    15481 attcataagc tgctcccggg cttgcaaaag cccatgtcca tatttttgat ggaggaaaca
    15541 ataggcaatc gcaggcggag caaacagtgg ataaaaaggc catttactgg acttttgtaa
    15601 tatcagtaca aaaatgcgat ccgcctcata acttgcgata atccggaagc atgccgcaaa
    15661 acggcgtctt gtgagacgcg tcgcaaattg acggttacat tacatttgtc cagcttttgg
    15721 cagatttgtc acatggcagg ccgttaccgt gcgctcttat cctgtaaaca gaaaaccgta
    15781 ccgggagaaa ggcaatggcc ctgattgtgg aatttatttg tgagctacct aacggcgtac
    15841 atgcgcgtcc ggcaagccac gttgaaacgc tgtgtaatac tttttcatct caaattgagt
    15901 ggcataacct gcgtactgac cgtaaaggca acgccaaaag cgctctggcg ctgattggca
    15961 ccgatacgct ggtgggtgat aactgccagc tactgatttc cggggccgac gaacaggaag
    16021 ggcaccagcg tttaagccaa tggctgcgcg atgaattccc ccactgcgat gcgccgctgg
    16081 cggaagttaa atctgacgaa ctggaaccac tgccggtttc actgaccaat ctgaatccgc
    16141 aaattatccg cgcccgcacc gtgtgcagcg gtagtgcagg cggcattctg acgccgatct
    16201 cttctttaga tctcaatgcg ctgagtaatc ttcccgcagc caaagacgtt gacgccgagc
    16261 aatccgcact ggaaaacggc ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg
    16321 atagcgacgg tgctaccagc gcgattctgg aagcgcaccg atccctggct ggcgatactt
    16381 ccctgcacga acatttactg gcaggcgtca gcgccggatt aagctgcgcc gaagcgattg
    16441 tcgccagtgc gaatcacttt tgcgaagagt ttgcccgttc cagcagcaga tacctgcaag
    16501 aacgtgccct ggacgtacgc gacgtctgct tccagttact ccagcaaatc tacggtgagc
    16561 aacgcttccc ggcaccgggc aaactgacgc agtccgccat ttgtatggct gatgaactga
    16621 cccccagcca gttcctcgaa ctggataaaa atcacctcaa aggattgtta ctgaaaagcg
    16681 gcggcaccac ctcacatacg gtgatccttg cccgttcgtt caacattccg acgctggttg
    16741 gtgtggatat tgatgccctc actccgtggc agcatcaaac gatttatatc gacggcaacg
    16801 ccggggcgat tgtggttgag ccaggagaag ccgtagctcg ttattatcag caagaagccc
    16861 gcgtacagga cgccctgcgt gagcaacagc gtgtctagct gacccaacaa gcccgtaccg
    16921 ctgacggtat ccgcattgaa attgccgcta acatcgctca ctccgtggaa gcgcaggccg
    16981 cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac tgaaatgctc tatatggatc
    17041 gcaccagtgc accgggcgaa agcgagctgt acaacatttt ttgtcaggcg ctggagtctg
    17101 ccaacggacg cagcattatt gtgcgcacta tggacattgg cggcgacaaa cccgttgatt
    17161 atctaaacat tcccgcagag gcaaacccgt tcctcggtta tcgcgccgtg cgtatttatg
    17221 aagaatacgc gtcgttattt accacacaac tacgctcgat cctgcgcgcc tccgctcacg
    17281 gcagcctgaa aatcatgatc ccgatgatct cctcaatgga agagatctta tgggtgaaag
    17341 aaaaactggc agaagccaaa cagcaactac gtaacgaaca cattccgttt gatgagaaga
    17401 tccagctcgg cattatgctg gaagtgccgt cggtgatgtt catcatcgat caatgctgcg
    17461 aagagattga tttctttagt attggtagta atgacctgac gcaatatctg ctggcagtgg
    17521 atcgcgataa cgctaaggtt actcgtcact acaacagcct gaatccggca ttcttgcggg
    17581 cgctcgatta cgacgtgcag gcggtgcatc gccagggtaa atggattggt ctgtgcggtg
    17641 agctgggagc gaaaggttcc gtgctgccgt tgctggtcgg cttagggctg gatgaactca
    17701 gcatgagcgc accatcaatt ccggcggcga aagcgcggat ggcgcaactt gatagccgtg
    17761 agtgccgcaa gttgctcaac caggcaatgg cctgccgtac ttcgctggaa gtggaacacc
    17821 tgctggcgca attccgcatg acccaacagg acgcaccgct ggtcaccgcc gagtgcatca
    17881 cgctggaaag tgactggcgc agcaaagaag aagtgctcaa aggcatgacc gataacctgc
    17941 tgctggcggg ccgctgccgc tatccgcgta aactggaagc cgacttatgg gcgcgcgagg
    18001 ccgttttctc taccggtctg ggctttagtt ttgccattcc acacagcaaa tcagaacaca
    18061 ttgagcaatc caccatcagc gtggcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg
    18121 aagcgcaatt catcattatg ttaaccctga acaaacacgc tgcgggcgat cagcacatgc
    18181 gcattttctc gcgcctcgct cgccgcatca tgcacgaaga attccgtaac gcgctggtta
    18241 acgccgcctc tgccgacgct atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg
    18301 aaacatcatg gaactgtatc tggacaccgc taacgtcgca gaagtcgaac gtctggcacg
    18361 catattcccg attgccgggg tgacaactaa cccgagcatt atcgctgcca gcaaggagtc
    18421 catctgggaa gtgctgccgc gtctgcaaaa agcgattggt gatgagggca ttctgtttgc
    18481 tcagaccatg agccgcgacg cgcaggggat ggtggaagaa gcgaagcgcc tgcgcgacgc
    18541 tattccgggt attgtggtga aaatcccggt gacttccgaa ggtctggcag caattaaaat
    18601 actgaaaaaa tagggtatta ctacacttgg caccgctgta tatagcgccg cagaagggtt
    18661 attagccgca ctggcagggg caaaatacgt tgctccgtat gttaaccgcg tagatgccca
    18721 gggcggggac ggcattcgta cggttcagga gctgcaaacg ctgttagaaa tgcacgcgcc
    18781 agaaagcatg gtgctggcag ccagctttaa aacgccgcgt caggcgctgg actgtttact
    18841 ggcaggatgt gaatccatca ccctgccctt agatgtagcg caacaaatgc tcaacacccc
    18901 tgcggtagag tcagctatag agaagttcga acacgactgg aatgccgcat ttggcactac
    18961 tcatctctaa aggagcaatt atggaccgca ttattcaatc accgggtaaa tacatccagg
    19021 gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc gctggcagaa cgctggttag
    19081 tggtgggtga caaatttgtt ttagtttttg ctcaatccac tgtcgagaaa agctttaaag
    19141 atgctggact ggtagtagaa attacgccgt ttggcggtga atgttcgcaa aatgagatcg
    19201 accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc aattctcggt atcggtggcg
    19261 gaaaaaccct cgatactgcc aaagcactgg cacatttcat gggtgttccg gtagcgatcg
    19321 caccgactat cgcctcgacc gatgcaccgt gcagcgcatt gtctgttatc tacaccgatg
    19381 agggtgagtt tgaccgctat ctgctgttgc caaataaccc gaatatggtc attgtcgaca
    19441 ccaaaatcgt cgcgggcgca cctgcacgtc tgttagcagc gggtatcggc gatgcgctgg
    19501 caacctggtt tgaagcgcgt gcttgctctc gtagcggcgc gaccaccatg gcgggcggca
    19561 agtgcaccca ggcggcgctg gcactggctg aactgtgcta caacaccctg ctggaagaag
    19621 gcgaaaaagc gatgcttgcc gccgaacagc atgtagtaac cccggcgctg gagcgcgtga
    19681 ttgaagcgaa cacctatttg agcggcgttg gttttgaaag cggcggctgg cggcggctca
    19741 cgcggtgcat aacggcctga ccgctatccc ggacgcgcat cactattatc acggtgaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcg
    20521 cagcttacct atcctggcgg tgatgttccg caagaacgcg gtgtatgttc cgatatggtg
    20581 atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga agatatggcg
    20641 aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag caacatcgat
    20701 caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac acgccccatc
    20761 agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt agataacggg
    20821 ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc gctggtgata
    20881 cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg gatggtgggg
    20941 cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat tcagatgctt
    21001 tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag cacaatcagc
    21061 acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca ggaaaagagt
    21121 acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg caagtttttc
    21181 agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact gtaggccagc
    21241 gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa agcgaccaca
    21301 ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa atccacgcgc
    21361 ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact aacaccggag
    21421 aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg ctgcaacacc
    21481 aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg caggcgttca
    21541 ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct caggatcgcc
    21601 accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat cccgcaggtt
    21661 tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc gcgaccacgt
    21721 aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc ggtaaagtcg
    21781 aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact ccagataagt
    21841 gtgagcacaa ccaagccact gattgccagt gggttgctct ttcctgcggc agacatgaga
    21901 gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt cagcgactgc
    21961 ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc gaggttcatc
    22021 actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc gctgctggcg
    22081 taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc cgcacggctg
    22141 gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc tttcgattcg
    22201 tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc attgctcaat
    22261 acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc acccagtaca
    22321 cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg ctgtgctttg
    22381 tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt acggaaaccg
    22441 tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg acgcgcatca
    22501 acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc ggctttctca
    22561 acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc agactctttc
    22621 tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc ccagtcgcgc
    22681 tgcggcatta atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc accacggaag
    22741 gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc agaatccgca
    22801 atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg ctgcggcagc
    22861 ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg agatttcggc
    22921 cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt gaacgcctgc
    22981 ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg cagcgtcagg
    23041 tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc cgggataatt
    23101 tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt ctgtacccac
    23161 tcatacttga acagattctc gaagaaatag ttgctccact ggatcggcgt ctgggtccag
    23221 actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta agtgctcgcc
    23281 cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac gtttgatgtc
    23341 ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc cacggtttct
    23401 tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc agaaagcggt
    23461 tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc ggttgcaccc
    23521 agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt ttcatcaccc
    23581 cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa accgaaggta
    23641 cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc ggcccaggag
    23701 attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt atcgaggctt
    23761 acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc cgcgccgccg
    23821 cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat aaacagaccg
    23881 gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag ggctttcaga
    23941 tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc aaagtcctca
    24001 cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag ttgatttggc
    24061 caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc ctgatggaac
    24121 gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat caatgtgctc
    24181 ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa ttgaattaat
    24241 ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta gatcaaattg
    24301 atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat aaaaaagggt
    24361 tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc ttattttccc
    24421 gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt
    24481 tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc aaatcgcgta
    24541 actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt cacggcttaa
    24601 aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt cggcatcgtc
    24661 atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg tcatgtcggc
    24721 aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga tttccacatc
    24781 aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat cgaagaagaa
    24841 ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa gcaaatccgc
    24901 ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata tatcgaaatc
    24961 tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct taccacttcc
    25021 cggcggcaga tcgccacgca gtgccacgat atgacgaata ccgttattcc agtagtcgcg
    25081 tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg gtgccgcttc
    25141 cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga
    25201 gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc gatcgatgga
    25261 gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga acgaaacgtt
    25321 aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc tggcgtgaaa
    25381 aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt gcacatctat
    25441 ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa ccgaaaaatg
    25501 accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg taagaaaaag
    25561 aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt acttattgct
    25621 tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat tcaatatatt
    25681 tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt tctgaaatta
    25741 caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga cgtcgcgccc
    25801 agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt cgatggcaaa
    25861 gacgttatcg catggcagca gtgatgccag cggatagtct tcacgcaccg cctccacgcc
    25921 cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca gccccatttc
    25981 gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat tttcaaagaa
    26041 atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc gattccacac gtacctggtc
    26101 cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca ttacgtcttt
    26161 gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg cctgatccac
    26221 cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga gcgtgccgga
    26281 gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc gcacggtatg
    26341 gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc cggttttttc
    26401 gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca gtttgttggc
    26461 actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa gctgctggct
    26521 ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc acaggaaaag
    26581 cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc ggctggcgtc
    26641 cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca gcacaaattc
    26701 aaagccggta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca gccagcgaga
    26761 gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc ggaagacgga
    26821 ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg ccaccagact
    26881 gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt gctgccagaa
    26941 gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca ccagcgccag
    27001 cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt tcagcgcact
    27061 gtcggccact tctgaggtgt agcaaaattg caacaactgg cgatcgttat gcacgccaac
    27121 cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat gcgccagttt
    27181 gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt ggctggtgac
    27241 aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac cttgatccgg
    27301 cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct gtaaagtacg
    27361 ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca gacgcagcaa
    27421 cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc cggcgacgtc
    27481 gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc
    27541 ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga taaacccggt
    27601 caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg ggtaagaaag
    27661 cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac gggcatcaag
    27721 ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg ccgaccacac
    27781 ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt cgagcaacgc
    27841 cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt cttcagcggg
    27901 tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa cctgatgcgc
    27961 agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag tggtgctacc
    28021 ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa tgcccgcgac
    28081 acgcaaataa cacttcacat cagccagact actgccacca aatttatgca gctgacgacc
    28141 tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc ccggaagcca
    28201 ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat acgcagcagc
    28261 gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg tgtcatggtt
    28321 gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt aaacaacgac
    28381 agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa actcaacatt
    28441 gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc cggcagcgac
    28501 gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat cgcctgcgcg
    28561 ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag cagcagatag
    28621 ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag ttcagtgaca
    28681 acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc gttcagatat
    28741 ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg taatgccggg
    28801 cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag atggcagatt
    28861 ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag taccagtttg
    28921 ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg
    28981 cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc gtagcagtcg
    29041 tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag gtgaatcgcg
    29101 gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc cagcgcacgc
    29161 tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg cggttcatta
    29221 aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca accatactgt
    29281 tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt catgtgatga
    29341 agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt gttaatggac
    29401 gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc
    29461 gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac ggtaacgcct
    29521 gtacggtaaa ctatgcgggt ttacggtcag tacccacata aattgtgggg tctggtctca
    29581 atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa tatatcagcc
    29641 catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt tccattcctc
    29701 ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc
    29761 acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt
    29821 tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag
    29881 agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc ggaaaagagc
    29941 ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat attgcaattt
    30001 attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc ggcataaaca
    30061 acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact atgaaaaaca
    30121 ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag ccgcaaactg
    30181 gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa ctggcaaccc
    30241 attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc tacgtttttg
    30301 aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat ggtgaaaagg
    30361 gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag caaaaactga
    30421 cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca tggagcgacg
    30481 atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg ggaatgcgcg
    30541 tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc caggcgaagc
    30601 tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca ccacaagccg
    30661 ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt tcgtggtgat
    30721 acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct
    30781 tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca
    30841 acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg
    30901 tcatgaccaa cggtggagcg gatttttatt acgttaccgc aagagcagct agcagtaatt
    30961 tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg
    31021 ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca
    31081 tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac
    31141 tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc
    31201 gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa
    31261 ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc
    31321 atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga gctaaaagcg
    31381 gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg attgctgcta
    31441 tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt
    31501 ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa
    31561 caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca acaggcgctg
    31621 gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga atttaatgat
    31681 gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag
    31741 tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa
    31801 caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg
    31861 gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc
    31921 gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc
    31981 gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg tctgaacgac
    32041 agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat tgtgatcggc
    32101 acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgacgaagag
    32161 cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg
    32221 tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa
    32281 acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg ccgggcgggg
    32341 aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt gcaggcaggt
    32401 ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc
    32461 ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg cggctggatt
    32521 gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc
    32581 tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg
    32641 cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgctgttc
    32701 cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa
    32761 cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac acaaatgctg
    32821 gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg
    32881 ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac ccaggtcgct
    32941 ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca ccatccggaa
    33001 catcctctgt tgcaaacgtt gctctataaa ggctacgatg cctttgccga acaggcgctg
    33061 gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt gcgtgcggaa
    33121 gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctctcc
    33181 agcccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact ggcacctaaa
    33241 cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt gcgcttgcaa
    33301 cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg caaggtgaaa
    33361 tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag gcggatcgaa
    33421 aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg
    33481 ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag
    33541 gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca
    33601 aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc
    33661 aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta
    33721 tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct
    33781 gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat
    33841 atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt
    33901 tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg
    33961 ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg
    34021 aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt
    34081 atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccac
    34141 tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga attatggttg
    34201 atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcaatgcaac
    34261 agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat gtgatggcgc
    34321 tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa
    34381 tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc
    34441 agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc
    34501 aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac
    34561 gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta
    34621 agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat
    34681 tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag
    34741 caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg
    34801 acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg
    34861 caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac
    34921 attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt
    34981 ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg
    35041 caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa tgaacagacg
    35101 ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag
    35161 caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg
    35221 tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc tactcagcag
    35281 ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa
    35341 gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa
    35401 cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt
    35461 gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt
    35521 gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag
    35581 atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc
    35641 ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt
    35701 aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca
    35761 tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg
    35821 tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt
    35881 ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggttaaag
    35941 ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac
    36001 tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc
    36061 agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc
    36121 gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag gcgttggata
    36181 ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca
    36241 aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata
    36301 agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct
    36361 ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga
    36421 tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga
    36481 atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac gtcggtaagg
    36541 aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg
    36601 ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc
    36661 tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa ccttccgctg
    36721 ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga
    36781 tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc atggaagaga
    36841 tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta
    36901 agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga
    36961 acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta
    37021 tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg
    37081 aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg
    37141 ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac
    37201 cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa ctgcaggcac
    37261 tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt
    37321 acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaaac
    37381 gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt
    37441 tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg
    37501 gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag
    37561 atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat
    37621 ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa
    37681 ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta
    37741 tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg
    37801 cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg
    37861 cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg
    37921 tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct
    37981 ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga
    38041 ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg
    38101 gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg
    38161 ggagctggta tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat
    38221 gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc
    38281 gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc
    38341 atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc
    38401 atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt
    38461 atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg
    38521 gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat
    38581 aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata
    38641 ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg
    38701 tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg
    38761 ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta
    38821 cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt
    38881 tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg
    38941 tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat
    39001 ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt
    39061 ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac
    39121 cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc accgcaaggt
    39181 gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga aagatgaagc
    39241 gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct
    39301 gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt
    39361 gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca
    39421 gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg
    39481 acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat
    39541 taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg
    39601 cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc
    39661 tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct
    39721 aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg
    39781 acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttaa
    39841 cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact
    39901 cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc
    39961 ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct
    40021 ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc
    40081 atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg
    40141 tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc
    40201 ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc
    40261 gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc
    40321 tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt
    40381 accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc
    40441 ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc
    40501 ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctag
    40561 ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg
    40621 ccgcttttca gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt
    40681 ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca
    40741 gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaatat
    40801 atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc
    40861 aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta
    40921 gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg
    40981 aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc
    41041 actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc
    41101 cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc
    41161 agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc
    41221 gaccatgtgg aaggctctca cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt
    41281 gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac
    41341 gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa
    41401 gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt
    41461 cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac
    41521 cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat
    41581 ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc
    41641 ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg
    41701 gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac
    41761 gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt
    41821 ccggcattta ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc
    41881 ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct
    41941 tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc
    42001 ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt
    42061 ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat
    42121 ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg
    42181 attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc
    42241 tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta atgtcaggca
    42301 cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct tatcaggcct
    42361 acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc cggcatcacg
    42421 cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac ccttcccctg
    42481 tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat
    42541 cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt taggacgcgg
    42601 cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt
    42661 caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac cgatgctcta
    42721 cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg ttgatccgat
    42781 tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg
    42841 cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga ttgtcgggcc
    42901 gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc gcaatgtagc
    42961 ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta aaccacgcca
    43021 cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggacgg
    43081 cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg
    43141 tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat tagatggcga
    43201 catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg
    43261 cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg gtaaagtatt
    43321 gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg gtattaccaa
    43381 aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg aaacgctgct
    43441 gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc tggatcgcaa
    43501 agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc cttccagtcc
    43561 ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat caaaacagga
    43621 gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc
    43681 cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc aatttaccaa
    43741 gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc
    43801 cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg
    43861 attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt
    43921 tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg gtacagcgat
    43981 tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca aaaatctggt
    44041 cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact
    44101 ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg aaacggcagc
    44161 ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg aaagcgcgat
    44221 tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt
    44281 gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg
    44341 accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg
    44401 atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat caaacggttg
    44461 ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc
    44521 agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata
    44581 tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct
    44641 tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt
    44701 aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac cagtaaggca
    44761 tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc
    44821 taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct
    44881 ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttat
    44941 aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg
    45001 tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt
    45061 tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta
    45121 cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat
    45181 aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga
    45241 tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa
    45301 ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta
    45361 ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc gccttgtgtg
    45421 ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc
    45481 cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag cagcatccac
    45541 gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaaca
    45601 gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt ctgaatatcg
    45661 agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc
    45721 caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg
    45781 gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac
    45841 agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt
    45901 acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca
    45961 ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctcc
    46021 atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact gaacgaattt
    46081 cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag taaaattaac
    46141 gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa
    46201 atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa agctcaggaa
    46261 tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa
    46321 aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga gacagaaggt
    46381 gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg
    46441 attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct
    46501 ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt
    46561 cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact
    46621 ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc
    46681 tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg
    46741 cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc
    46801 tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc ggcgctcagt
    46861 acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac gaactgattg
    46921 cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc atcggtgaaa
    46981 ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag atcgacgcgg
    47041 tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac gaacctgtat
    47101 gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt cacaaattca
    47161 tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc attcagtacg
    47221 gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac atcgacggcg
    47281 cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt aaagctgcag
    47341 aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc acttactttc
    47401 cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc ctcggctgaa
    47461 gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga attatcactc
    47521 tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg cacttcttct
    47581 gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg
    47641 tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat gttcgccaga
    47701 tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat atgaaaatgg
    47761 ttttgcgtat gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc
    47821 tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc
    47881 gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt tggcatcaac
    47941 agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt gggtttgact
    48001 tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc ctcaaggaca
    48061 atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc
    48121 acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc
    48181 ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc
    48241 gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga tctgatcgaa
    48301 cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc
    48361 aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta cttcagcgtc
    48421 gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt
    48481 caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat cgaccaccga
    48541 caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt tatctttccc
    48601 cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat cgccaatccc
    48661 gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt cgacgttttt
    48721 atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc
    48781 gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg gattaggcgt
    48841 gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct tcggattgcc
    48901 cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt
    48961 gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc
    49021 cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt tacctgaacc
    49081 accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt gggcgctgaa
    49141 tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg
    49201 aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta cgccccactt
    49261 gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac
    49321 caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt gagggattaa
    49381 aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca tgccggatgc
    49441 ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg cagaattcat
    49501 gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa
    49561 aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcac
    49621 ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt
    49681 cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag
    49741 cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt
    49801 ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt
    49861 cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga
    49921 tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaactacaa
    49981 cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa taacggccca
    50041 tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg
    50101 cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt
    50161 cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc agacgcattg
    50221 cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca cgttttttca
    50281 ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac
    50341 cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct agctgtacca
    50401 tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc agaccttctg
    50461 tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc
    50521 ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa
    50581 atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata
    50641 acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa
    50701 atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg
    50761 gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa
    50821 tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca
    50881 tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag
    50941 cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt tcttcatcag
    51001 gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata
    51061 aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccaag
    51121 acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca
    51181 tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga aacgagacaa
    51241 ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct ggatctgtca
    51301 tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt gccgaaccgg
    51361 agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt
    51421 gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc aacgacgcgc
    51481 cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc
    51541 atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca atcgccgccc
    51601 gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt
    51661 ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa
    51721 ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac agggtttact
    51781 actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt tgccattgcg
    51841 tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc gcttgctgct
    51901 ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc tgacgagcaa
    51961 tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca aaattttctg
    52021 cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc
    52081 gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa cttatgccgt
    52141 cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc
    52201 ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag gccatgacgg
    52261 cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga
    52321 gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg
    52381 attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc
    52441 ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa
    52501 ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc
    52561 attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa
    52621 gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt
    52681 gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat
    52741 cgtgagctgg tggcacgtat tcgcgcaatc ctgcgccgtt cgcactggag cgagcaacag
    52801 caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc
    52861 cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg
    52921 ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa
    52981 gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg
    53041 cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc
    53101 ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct
    53161 tctggctgac gctggtgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac
    53221 gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg
    53281 tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc
    53341 gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc
    53401 gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg
    53461 ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct
    53521 ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc agtagttctc
    53581 aatccgattt cattaactta ttgtttgacc gcccgctatt actgctgatt gtcaccatgt
    53641 tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc
    53701 tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag
    53761 cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg
    53821 agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc
    53881 cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt gaaagcaagg
    53941 aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg
    54001 tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt
    54061 tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga
    54121 cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg
    54181 cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct
    54241 ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc
    54301 cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg
    54361 aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg
    54421 gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc
    54481 tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg
    54541 gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg
    54601 ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt
    54661 tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga
    54721 tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg
    54781 acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta
    54841 tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa
    54901 tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc
    54961 cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt tgagagattt
    55021 tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg cacccagtag
    55081 agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg aacctttttc
    55141 tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg
    55201 attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca
    55261 tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta
    55321 ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc
    55381 ggacgaacgt tagttcatcc ggctgtggg
//
LOCUS       DAJTRW010000017        53200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRW010000017 DAJTRW010000000
VERSION     DAJTRW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(11..322)
                     /locus_tag="OO326_001107"
     CDS             complement(11..322)
                     /locus_tag="OO326_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5965568.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO326_001108"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO326_001108"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO326_001109"
     CDS             701..1054
                     /locus_tag="OO326_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5965569.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO326_001110"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO326_001110"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5965570.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO326_001111"
     CDS             complement(2810..3280)
                     /locus_tag="OO326_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5965571.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO326_001112"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO326_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO326_001113"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO326_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5965572.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO326_001114"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO326_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5965573.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /locus_tag="OO326_001115"
     CDS             complement(5282..8431)
                     /locus_tag="OO326_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5965574.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO326_001116"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO326_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5965575.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="OO326_001117"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="OO326_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5965576.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="OO326_001118"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="OO326_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5965577.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO326_001119"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO326_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5965578.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO326_001120"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO326_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5965579.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="OO326_001121"
     CDS             14910..15287
                     /locus_tag="OO326_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5965580.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="OO326_001122"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="OO326_001122"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5965581.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="OO326_001123"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="OO326_001123"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5965582.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="OO326_001123"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="OO326_001124"
     CDS             18098..18427
                     /locus_tag="OO326_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5965583.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="OO326_001125"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="OO326_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5965584.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="OO326_001126"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="OO326_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5965585.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="OO326_001127"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="OO326_001127"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5965586.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="OO326_001128"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="OO326_001128"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5965587.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO326_001129"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO326_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="OO326_001130"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="OO326_001130"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5965588.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /locus_tag="OO326_001131"
     CDS             complement(25480..27156)
                     /locus_tag="OO326_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5965589.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO326_001132"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO326_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5965590.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="OO326_001133"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="OO326_001133"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5965591.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO326_001134"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO326_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5965592.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="OO326_001135"
     CDS             complement(31204..31998)
                     /locus_tag="OO326_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5965593.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="OO326_001136"
     CDS             32136..32477
                     /locus_tag="OO326_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5965594.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO326_001137"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO326_001137"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5965595.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="OO326_001138"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="OO326_001138"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5965596.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="OO326_001139"
     CDS             36393..37685
                     /locus_tag="OO326_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5965597.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="OO326_001140"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="OO326_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5965598.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="OO326_001141"
     CDS             complement(38218..38676)
                     /locus_tag="OO326_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5965599.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="OO326_001142"
     CDS             complement(38673..39590)
                     /locus_tag="OO326_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5965600.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="OO326_001143"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="OO326_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5965601.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="OO326_001144"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="OO326_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5965602.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO326_001145"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO326_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5965603.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO326_001146"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO326_001146"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5965604.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO326_001147"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO326_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /locus_tag="OO326_001148"
     CDS             43549..44235
                     /locus_tag="OO326_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5965605.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /locus_tag="OO326_001149"
                     /pseudo
     CDS             44232..46647
                     /locus_tag="OO326_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO326_001150"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO326_001150"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5965606.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO326_001151"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO326_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5965607.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OO326_001152"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OO326_001152"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5965608.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OO326_001153"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OO326_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5965609.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OO326_001154"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OO326_001154"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5965610.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53200
                     /locus_tag="OO326_001155"
     CDS             52938..>53200
                     /locus_tag="OO326_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5965611.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT        12826 a        13688 c        14013 g        12673 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc
//
LOCUS       DAJTRW010000018        52599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRW010000018 DAJTRW010000000
VERSION     DAJTRW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..356
                     /locus_tag="OO326_001156"
     CDS             <1..356
                     /locus_tag="OO326_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5965612.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO326_001157"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO326_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5965613.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO326_001158"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO326_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5965614.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="OO326_001159"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="OO326_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5965615.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO326_001160"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO326_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5965616.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO326_001161"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO326_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5965617.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="OO326_001162"
     CDS             6879..7283
                     /locus_tag="OO326_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5965618.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="OO326_001163"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="OO326_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5965619.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="OO326_001164"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="OO326_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5965620.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO326_001165"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO326_001165"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5965621.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO326_001166"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO326_001166"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5965622.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="OO326_001167"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="OO326_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="OO326_001168"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="OO326_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5965623.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="OO326_001169"
     CDS             complement(13135..13431)
                     /locus_tag="OO326_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5965624.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="OO326_001170"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="OO326_001170"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5965625.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="OO326_001171"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="OO326_001171"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5965626.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="OO326_001172"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="OO326_001172"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5965627.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="OO326_001173"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="OO326_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5965628.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="OO326_001174"
     CDS             complement(18688..19374)
                     /locus_tag="OO326_001174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5965629.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /locus_tag="OO326_001175"
     CDS             complement(19774..19914)
                     /locus_tag="OO326_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965630.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="OO326_001176"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="OO326_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5965631.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="OO326_001177"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="OO326_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5965632.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="OO326_001178"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="OO326_001178"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5965633.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="OO326_001179"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="OO326_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5965634.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="OO326_001180"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="OO326_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5965635.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="OO326_001181"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="OO326_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5965636.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="OO326_001182"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="OO326_001182"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5965637.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="OO326_001183"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="OO326_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="OO326_001184"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="OO326_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5965638.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="OO326_001185"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="OO326_001185"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5965639.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="OO326_001186"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="OO326_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5965640.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /locus_tag="OO326_001187"
     CDS             complement(31513..31680)
                     /locus_tag="OO326_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965641.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="OO326_001188"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="OO326_001188"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5965642.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="OO326_001189"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="OO326_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5965643.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="OO326_001190"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="OO326_001190"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5965644.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="OO326_001191"
     CDS             35472..36116
                     /locus_tag="OO326_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5965645.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="OO326_001192"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="OO326_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="OO326_001193"
     CDS             37191..37454
                     /locus_tag="OO326_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5965646.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="OO326_001194"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="OO326_001194"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5965647.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="OO326_001195"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="OO326_001195"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5965648.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="OO326_001196"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="OO326_001196"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5965649.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="OO326_001197"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="OO326_001197"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5965650.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="OO326_001198"
     CDS             42152..43693
                     /locus_tag="OO326_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5965651.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="OO326_001199"
     CDS             43665..44528
                     /locus_tag="OO326_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5965652.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="OO326_001200"
     CDS             complement(44742..45524)
                     /locus_tag="OO326_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5965653.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="OO326_001201"
     CDS             complement(45521..46594)
                     /locus_tag="OO326_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5965654.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="OO326_001202"
     CDS             complement(46716..46877)
                     /locus_tag="OO326_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5965655.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="OO326_001203"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="OO326_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5965656.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="OO326_001204"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="OO326_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5965657.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="OO326_001205"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="OO326_001205"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5965658.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="OO326_001206"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="OO326_001206"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5965659.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="OO326_001207"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="OO326_001207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5965660.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="OO326_001208"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="OO326_001208"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5965661.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12527 a        14233 c        13181 g        12658 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tagagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgattt catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac tggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatgg
//
LOCUS       DAJTRW010000019        49595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRW010000019 DAJTRW010000000
VERSION     DAJTRW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..1214
                     /locus_tag="OO326_001209"
     CDS             <1..1214
                     /locus_tag="OO326_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5965662.1"
                     /translation="EITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHPVIT
                     GHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEI
                     AINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLA
                     CAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTK
                     GMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVP
                     FNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNL
                     PDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLA
                     QFGVDIGEAAQ"
     gene            1211..2176
                     /gene="yggF"
                     /locus_tag="OO326_001210"
     CDS             1211..2176
                     /gene="yggF"
                     /locus_tag="OO326_001210"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5965663.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2198..2707
                     /gene="fumE"
                     /locus_tag="OO326_001211"
     CDS             2198..2707
                     /gene="fumE"
                     /locus_tag="OO326_001211"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5965664.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2704..3417
                     /locus_tag="OO326_001212"
     CDS             2704..3417
                     /locus_tag="OO326_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5965665.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3389..4066)
                     /locus_tag="OO326_001213"
     CDS             complement(3389..4066)
                     /locus_tag="OO326_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5965666.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4060..4737)
                     /locus_tag="OO326_001214"
     CDS             complement(4060..4737)
                     /locus_tag="OO326_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5965667.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4725..5108)
                     /locus_tag="OO326_001215"
     CDS             complement(4725..5108)
                     /locus_tag="OO326_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5965668.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5124..5432)
                     /locus_tag="OO326_001216"
     CDS             complement(5124..5432)
                     /locus_tag="OO326_001216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965669.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5433..6011)
                     /locus_tag="OO326_001217"
     CDS             complement(5433..6011)
                     /locus_tag="OO326_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5965670.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6034..6465)
                     /locus_tag="OO326_001218"
                     /pseudo
     CDS             complement(6034..6465)
                     /locus_tag="OO326_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6837..7856
                     /gene="epd"
                     /locus_tag="OO326_001219"
     CDS             6837..7856
                     /gene="epd"
                     /locus_tag="OO326_001219"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5965671.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7906..9069
                     /gene="pgk"
                     /locus_tag="OO326_001220"
     CDS             7906..9069
                     /gene="pgk"
                     /locus_tag="OO326_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5965672.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9284..10363
                     /gene="fbaA"
                     /locus_tag="OO326_001221"
     CDS             9284..10363
                     /gene="fbaA"
                     /locus_tag="OO326_001221"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5965673.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10554..11414
                     /gene="mscS"
                     /locus_tag="OO326_001222"
     CDS             10554..11414
                     /gene="mscS"
                     /locus_tag="OO326_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5965674.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11553..12188
                     /gene="argO"
                     /locus_tag="OO326_001223"
     CDS             11553..12188
                     /gene="argO"
                     /locus_tag="OO326_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5965675.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12281..13021
                     /locus_tag="OO326_001224"
     CDS             12281..13021
                     /locus_tag="OO326_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5965676.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13188..14138
                     /locus_tag="OO326_001225"
     CDS             13188..14138
                     /locus_tag="OO326_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5965677.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14080..15558)
                     /gene="scpC"
                     /locus_tag="OO326_001226"
                     /pseudo
     CDS             complement(14080..15558)
                     /gene="scpC"
                     /locus_tag="OO326_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15582..16367)
                     /gene="scpB"
                     /locus_tag="OO326_001227"
     CDS             complement(15582..16367)
                     /gene="scpB"
                     /locus_tag="OO326_001227"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5965678.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16378..17373)
                     /gene="meaB"
                     /locus_tag="OO326_001228"
     CDS             complement(16378..17373)
                     /gene="meaB"
                     /locus_tag="OO326_001228"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5965679.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17366..19510)
                     /gene="scpA"
                     /locus_tag="OO326_001229"
     CDS             complement(17366..19510)
                     /gene="scpA"
                     /locus_tag="OO326_001229"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5965680.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19714..20607)
                     /gene="argP"
                     /locus_tag="OO326_001230"
     CDS             complement(19714..20607)
                     /gene="argP"
                     /locus_tag="OO326_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5965681.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20990..21649
                     /gene="rpiA"
                     /locus_tag="OO326_001231"
     CDS             20990..21649
                     /gene="rpiA"
                     /locus_tag="OO326_001231"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5965682.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21905..23137
                     /gene="serA"
                     /locus_tag="OO326_001232"
     CDS             21905..23137
                     /gene="serA"
                     /locus_tag="OO326_001232"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5965683.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23366..23425
                     /gene="ibsC"
                     /locus_tag="OO326_001233"
     CDS             23366..23425
                     /gene="ibsC"
                     /locus_tag="OO326_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5965684.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23758..23817
                     /locus_tag="OO326_001234"
     CDS             23758..23817
                     /locus_tag="OO326_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5965685.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23918..24520)
                     /locus_tag="OO326_001235"
     CDS             complement(23918..24520)
                     /locus_tag="OO326_001235"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5965686.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24541..24724)
                     /gene="ssrS"
                     /locus_tag="OO326_001236"
     ncRNA           complement(24541..24724)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO326_001236"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24766..25095)
                     /gene="zapA"
                     /locus_tag="OO326_001237"
     CDS             complement(24766..25095)
                     /gene="zapA"
                     /locus_tag="OO326_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5965687.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25263..25841
                     /gene="ygfB"
                     /locus_tag="OO326_001238"
     CDS             25263..25841
                     /gene="ygfB"
                     /locus_tag="OO326_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5965688.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25867..27192
                     /gene="pepP"
                     /locus_tag="OO326_001239"
     CDS             25867..27192
                     /gene="pepP"
                     /locus_tag="OO326_001239"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5965689.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27189..28367
                     /gene="ubiH"
                     /locus_tag="OO326_001240"
                     /gene_synonym="visB"
     CDS             27189..28367
                     /gene="ubiH"
                     /locus_tag="OO326_001240"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5965690.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28390..29592
                     /gene="ubiI"
                     /locus_tag="OO326_001241"
                     /gene_synonym="visC"
     CDS             28390..29592
                     /gene="ubiI"
                     /locus_tag="OO326_001241"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5965691.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29666..29749)
                     /locus_tag="OO326_001242"
     CDS             complement(29666..29749)
                     /locus_tag="OO326_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965692.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30040..31134
                     /gene="gcvT"
                     /locus_tag="OO326_001243"
     CDS             30040..31134
                     /gene="gcvT"
                     /locus_tag="OO326_001243"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5965693.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31158..31547
                     /gene="gcvH"
                     /locus_tag="OO326_001244"
     CDS             31158..31547
                     /gene="gcvH"
                     /locus_tag="OO326_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5965694.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31666..34539
                     /gene="gcvP"
                     /locus_tag="OO326_001245"
     CDS             31666..34539
                     /gene="gcvP"
                     /locus_tag="OO326_001245"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5965695.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34806..35549
                     /locus_tag="OO326_001246"
     CDS             34806..35549
                     /locus_tag="OO326_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5965696.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35606..37039)
                     /gene="bglA"
                     /locus_tag="OO326_001247"
     CDS             complement(35606..37039)
                     /gene="bglA"
                     /locus_tag="OO326_001247"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5965697.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37084..37394
                     /locus_tag="OO326_001248"
                     /pseudo
     CDS             37084..37394
                     /locus_tag="OO326_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37558..38217
                     /locus_tag="OO326_001249"
     CDS             37558..38217
                     /locus_tag="OO326_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5965698.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38413..39393)
                     /gene="ygfZ"
                     /locus_tag="OO326_001250"
     CDS             complement(38413..39393)
                     /gene="ygfZ"
                     /locus_tag="OO326_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5965699.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39636..39902
                     /gene="sdhE"
                     /locus_tag="OO326_001251"
     CDS             39636..39902
                     /gene="sdhE"
                     /locus_tag="OO326_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5965700.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39883..40290
                     /locus_tag="OO326_001252"
     CDS             39883..40290
                     /locus_tag="OO326_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5965701.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40330..40851)
                     /gene="fldB"
                     /locus_tag="OO326_001253"
     CDS             complement(40330..40851)
                     /gene="fldB"
                     /locus_tag="OO326_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5965702.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40963..41859
                     /gene="xerD"
                     /locus_tag="OO326_001254"
     CDS             40963..41859
                     /gene="xerD"
                     /locus_tag="OO326_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5965703.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41884..42594
                     /gene="dsbC"
                     /locus_tag="OO326_001255"
     CDS             41884..42594
                     /gene="dsbC"
                     /locus_tag="OO326_001255"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5965704.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42600..44333
                     /gene="recJ"
                     /locus_tag="OO326_001256"
     CDS             42600..44333
                     /gene="recJ"
                     /locus_tag="OO326_001256"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5965705.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44424..45522
                     /gene="prfB"
                     /locus_tag="OO326_001257"
     CDS             join(44424..44498,44500..45522)
                     /gene="prfB"
                     /locus_tag="OO326_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5965706.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45532..47049
                     /gene="lysS"
                     /locus_tag="OO326_001258"
     CDS             45532..47049
                     /gene="lysS"
                     /locus_tag="OO326_001258"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5965707.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47092..47640)
                     /gene="idi"
                     /locus_tag="OO326_001259"
     CDS             complement(47092..47640)
                     /gene="idi"
                     /locus_tag="OO326_001259"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5965708.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47695..47766
                     /locus_tag="OO326_001260"
     CDS             47695..47766
                     /locus_tag="OO326_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965709.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47763..47888)
                     /locus_tag="OO326_001261"
     CDS             complement(47763..47888)
                     /locus_tag="OO326_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965710.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47890..49338)
                     /gene="uacT"
                     /locus_tag="OO326_001262"
     CDS             complement(47890..49338)
                     /gene="uacT"
                     /locus_tag="OO326_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5965711.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11974 a        12413 c        13353 g        11855 t
ORIGIN      
        1 cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga
       61 cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc
      121 atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca
      181 ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg
      241 gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat atgattattc
      301 ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg
      361 cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat tatcacacca
      421 cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg
      481 cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt ggcatacaac
      541 cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg cagaagctgc
      601 tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga
      661 tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc gatgacattt
      721 ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg gcggaagatg
      781 gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg tttaatttct
      841 acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca acggacgaca
      901 tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt atggtgaccc
      961 atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat atccctggcg
     1021 gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg
     1081 aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg
     1141 gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg
     1201 aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt
     1261 ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac
     1321 cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg
     1381 agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc
     1441 agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga tggggcagag
     1501 caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg cgcccgatat
     1561 gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc tgtctttgcc
     1621 tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg
     1681 catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa cacagctggg
     1741 cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca
     1801 ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg tgatttctgc
     1861 ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact tttgtcaggc
     1921 taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgtaaggc
     1981 aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg ggaacgatat
     2041 cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc aacagacggc
     2101 gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat
     2161 agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga cagaagatga
     2221 tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc
     2281 tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga
     2341 gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga
     2401 tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt atgccgatat
     2461 cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc ccggatttgc
     2521 cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca atgcgacgct
     2581 ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt
     2641 tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac
     2701 gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa
     2761 gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca gacggtaaat
     2821 ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa atctacccta
     2881 accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat tcaggcgctc
     2941 ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt gcgccccttc
     3001 aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg ccaggtggtg
     3061 gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt tgaagatgcg
     3121 ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct ggacgacgag
     3181 aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc gccagctgct
     3241 gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct ggcagatgca
     3301 gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga agagagtctg
     3361 cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaacgt
     3421 tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg gtaaatgcgg
     3481 cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc agccagcttt
     3541 caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agtaatagtg
     3601 gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ttatgcagat
     3661 ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca gcatgggtac
     3721 gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg atctcgtcag
     3781 cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca acctttgccg
     3841 cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc gtaccggctg
     3901 tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg ccattgtcac
     3961 cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg
     4021 ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg gctggagcgc
     4081 tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc gatgacgttc
     4141 aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat
     4201 ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga gctttggctg
     4261 catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga gtgttgccgg
     4321 gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa tccgcgccat
     4381 aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct
     4441 aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga
     4501 ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg tgcctgttat
     4561 ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc
     4621 actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca
     4681 gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg taaccatatc
     4741 cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac gcgttgtagg
     4801 gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata
     4861 tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag cggaataatt
     4921 aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggca
     4981 cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac caataacggc
     5041 ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc gcgaataaaa
     5101 cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc cagaatcagc
     5161 aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc gcttagccat
     5221 tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa catcagccag
     5281 acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa aaaggtcgcc
     5341 gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca ggccgctaat
     5401 gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat tgccggaaaa
     5461 tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac cgctgtaaga
     5521 attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc
     5581 cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac accgccgaac
     5641 agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgctg
     5701 acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc ggagaccagg
     5761 ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag caatccccag
     5821 agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag taccgcgcta
     5881 ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc cagttgcccg
     5941 ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact ggaaaagtgg
     6001 ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa tatgcaactc
     6061 gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa ggctaccgat
     6121 caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt
     6181 gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca gcgtgccgtt
     6241 tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc cggcatagcg
     6301 caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat gaagttgatg
     6361 ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga ggcgcagtgc
     6421 gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag tactccgaaa
     6481 ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata taagtcaggg
     6541 tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc aaagtggaat
     6601 aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct ttctttgact
     6661 ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt
     6721 aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccggacga
     6781 tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga
     6841 ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt
     6901 atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg gcggatgctg
     6961 cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca tgggaagtac
     7021 gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg catgaacgtt
     7081 cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg
     7141 tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa aaagtgctct
     7201 tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc
     7261 aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat tgcataattc
     7321 ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg accacaattc
     7381 actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg cgtcgcaccc
     7441 gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt atcacacgat
     7501 tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca accataaatg
     7561 tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc
     7621 tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc
     7681 cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa
     7741 cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct
     7801 ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg
     7861 caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag
     7921 atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca
     7981 gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa
     8041 ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa
     8101 ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg
     8161 tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa
     8221 ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaaccctg
     8281 tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac
     8341 cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg
     8401 ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc tcgcccgatg
     8461 gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga ctccctgtct
     8521 aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat cgcggcacaa
     8581 ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc taaacgtctg
     8641 ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa
     8701 actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca gatcctggat
     8761 atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg
     8821 tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg
     8881 gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca
     8941 gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca
     9001 ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct
     9061 aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc ttttagagca
     9121 cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc
     9181 caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt
     9241 gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga
     9301 tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa
     9361 agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt
     9421 actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc
     9481 ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg
     9541 cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat
     9601 cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc
     9661 gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca tgatcgacct
     9721 gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg agcgtatgtc
     9781 caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt
     9841 ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg ttgattacgc
     9901 atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct tcggtaacgt
     9961 acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc gtgattctca
    10021 ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg
    10081 tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat
    10141 gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc
    10201 gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa
    10261 gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct
    10321 ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt
    10381 atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt cagatgagaa
    10441 atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga ctgcccgtca
    10501 taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat tgaatggaag
    10561 atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct aaccaggcgc
    10621 tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc gttggtttga
    10681 ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt aaaatcgatg
    10741 ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc tttacgctaa
    10801 tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta ctcggtgccg
    10861 caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc gctggcgtgt
    10921 tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc ggcgtagccg
    10981 gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac ggtaaaatta
    11041 tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc cgcgagccag
    11101 ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat caggttaagc
    11161 agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc gaaatgactg
    11221 tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc tggagcaaca
    11281 gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt gaatttgatg
    11341 ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg gtgaaagaag
    11401 acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc tcttattagt
    11461 tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc gatatagtct
    11521 ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt caaggtcttg
    11581 ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg atgaatcagg
    11641 gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc gatttggtcc
    11701 tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg tggttgctag
    11761 cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc gcttttaaaa
    11821 cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa ggcagatgga
    11881 aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac ctggatactt
    11941 ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt tggtttgcgc
    12001 tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc gcagcctggc
    12061 tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta gtgggatgtg
    12121 tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca caagccttgt
    12181 tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc ctccagaggt
    12241 cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca aagttatcgc
    12301 cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat tgccggatgg
    12361 accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca ttgccactct
    12421 tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac aggcagatga
    12481 gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa aagatatcag
    12541 ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa gtatcctgaa
    12601 aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca aactgaattc
    12661 cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt cgctgggcgt
    12721 ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata acgcgattca
    12781 tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat atagcgtgcg
    12841 ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag ccgatgccgc
    12901 gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg atcaggtcga
    12961 tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg cagcacaata
    13021 atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg cctccttttt
    13081 ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt ttcatcaagg
    13141 ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg gacattttta
    13201 tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt gcccgggcgg
    13261 cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg ctggaagaac
    13321 aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat aaagccggtc
    13381 aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt gataatgaaa
    13441 tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat acttatccga
    13501 caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg accgcccagc
    13561 cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag ttagatttta
    13621 ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg actatcagtg
    13681 aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag cgtcaagagc
    13741 aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg gctaacttcg
    13801 aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt atttatcgtc
    13861 cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg acggtgatcc
    13921 cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat gcgccgcaca
    13981 ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat accgccacga
    14041 tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca tcgagccggt
    14101 tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa tatgtccgcc
    14161 gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg cacaattatc
    14221 aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga tcccttgttc
    14281 ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca ttggcacgac
    14341 ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca gatacgcgtt
    14401 gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg ctacgtgcgt
    14461 tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga tgacgcccag
    14521 acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa tgcggctggc
    14581 aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg tcaggctgga
    14641 ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt cctgtagcac
    14701 ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg ccattaccgc
    14761 attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa tacgcccatg
    14821 cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc acatgggatt
    14881 ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg cgacaatctt
    14941 tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga agatcgacac
    15001 gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg caacgcgcgg
    15061 atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct aggtcggcgc
    15121 attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg atgcttcaat
    15181 gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca cttcgctcaa
    15241 atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac tggacgatgt
    15301 ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt cagcggcggc
    15361 gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct catgctgttc
    15421 gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg gggtaaagcc
    15481 gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg cggtcatcct
    15541 tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc aacgaaatta
    15601 ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct gtcataaacc
    15661 gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg tgcttcgccc
    15721 agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc agagatgtgg
    15781 tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg gttgaggatg
    15841 ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag ctctttgaca
    15901 atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata cgggacgccg
    15961 aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag atcggaactc
    16021 atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat gatcggtttc
    16081 gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga gagcggatcg
    16141 cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt ggatccactt
    16201 ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa cgcctgcata
    16261 agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt aaactcaatg
    16321 accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa aattccttta
    16381 atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc gcggtgagag
    16441 cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat cttcattcgc
    16501 gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca ccgattgttg
    16561 ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt cgatgatggc
    16621 gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac cccgtggctg
    16681 ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat gccgggcaat
    16741 ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat cagccacttc
    16801 catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt gcaacgagat
    16861 aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa ccgtttcgac
    16921 aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg ctcgctgact
    16981 ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct cggcacgcgc
    17041 caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg ggctgctggg
    17101 atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc caaaggcttc
    17161 aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca gggcgttacc
    17221 acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac gtgggtgacg
    17281 gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac gtaagcggcg
    17341 aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga ttcagtacgt
    17401 cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg ccgcgctctt
    17461 gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag atatcttcgc
    17521 gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga ccggcagcca
    17581 gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg atctcttcag
    17641 gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag gcgctggcga
    17701 tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga atgcgcgggc
    17761 gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg aactcggagg
    17821 ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac tggcttggca
    17881 ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg gtggcgcgaa
    17941 cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca
    18001 gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg cgttccagcg
    18061 aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg gtttcgtctt
    18121 cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg
    18181 actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct tcgatcgctt
    18241 tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt ttgacgattt
    18301 gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg tggcagagtt
    18361 ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag
    18421 gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg
    18481 cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt tcagtcaggc
    18541 tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa
    18601 atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg acgttcataa
    18661 acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca tcaattttca
    18721 gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta aatgctacct
    18781 gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg atggtattaa
    18841 atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc attgacggtt
    18901 ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg ttttgaatgg
    18961 tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata taaaacgcca
    19021 gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc agcgggatct
    19081 ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt
    19141 cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa
    19201 cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg gactcttttg
    19261 ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac atagtggcac
    19321 gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc agattatcga
    19381 gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg
    19441 agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc cactcctgct
    19501 cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc gcttacggtg
    19561 agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg
    19621 cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt gaggacaacg
    19681 cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga ctttgtgacc
    19741 ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag caaagcggtg
    19801 ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt caccgctggc
    19861 cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct ggcgagcaag
    19921 ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag gaggaagatc
    19981 gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg
    20041 cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg caaacggttt
    20101 tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac tcggcagcgc
    20161 ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt cctgagtacg
    20221 ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca caggagccag
    20281 tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg
    20341 agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca cctggcgcag
    20401 cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac gcaccaacag
    20461 cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg attgtgtaat
    20521 gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg
    20581 taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc
    20641 tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc gggtaacatt
    20701 tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt gcatttcgct
    20761 tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct gttcgcaccg
    20821 tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc
    20881 attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt ctgaatcgct
    20941 ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca tgacgcagga
    21001 tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg gtaccattgt
    21061 tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca cgatgaaagg
    21121 ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga aaagcctcgg
    21181 cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg atggcgcaga
    21241 tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa
    21301 aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca agcaggttga
    21361 tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta gtgcagtggc
    21421 gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa
    21481 tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag cgatggaaaa
    21541 cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga
    21601 cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg
    21661 aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc acgcttttac
    21721 caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat ccaagaatta
    21781 cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg caatattatt
    21841 ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag acaggattgg
    21901 gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg tagaaggcgt
    21961 gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg aatttcacaa
    22021 aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact tcatcggcct
    22081 gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat
    22141 tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa agcgcgggat
    22201 cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg tgattggcga
    22261 actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg
    22321 gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta tcatcggcta
    22381 cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt atgtttactt
    22441 ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc atctttctga
    22501 cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa
    22561 tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga ttaatgcctc
    22621 gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca aacatctggc
    22681 gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc catttacctc
    22741 tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt cgactcagga
    22801 agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt ctgacaatgg
    22861 ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg gtggtcgtcg
    22921 tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca aaatcttcgc
    22981 cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga tgggttatgt
    23041 agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc
    23101 tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg aaaattaacg
    23161 ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc gcgttgcgca
    23221 ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc gttgtgttta
    23281 tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag ccctcaccga
    23341 agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga ttgtactgtt
    23401 actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat cctcccttac
    23461 ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat gcggggtgca
    23521 tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg ccgtaataga
    23581 tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg ctcgaagaaa
    23641 agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga tagctgggca
    23701 gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag aagggttatg
    23761 atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc ttattaacag
    23821 tcagtcacag gggaggagca atcctccctt acccttactc actaaattag gttaaagaat
    23881 caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag actttcgacg
    23941 gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt tccaccaact
    24001 gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt tgtaaggtcc
    24061 gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag gcgaccagcg
    24121 gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc agttttggct
    24181 catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc aggaacagca
    24241 aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga
    24301 gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat acagcgaccg
    24361 tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt tgacccattt
    24421 ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg cggatttctt
    24481 gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc agataagaag
    24541 ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca aggtgaatgt
    24601 gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc gcggagcgcc
    24661 acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa catctcagag
    24721 aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg
    24781 gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag
    24841 catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt tttcttgcgc
    24901 taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct
    24961 agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc
    25021 atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt ggatatcgac
    25081 gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc
    25141 atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg
    25201 gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc
    25261 ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac
    25321 aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg atatgtggcg
    25381 gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa ggcatggctt
    25441 tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc gatgccctgc
    25501 aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg
    25561 atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt ggcgttacgc
    25621 aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat ctgcgtaaca
    25681 tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag
    25741 agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc
    25801 aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag
    25861 agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc cctggtggag
    25921 caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc
    25981 gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa
    26041 ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag cgttctgttt
    26101 aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc
    26161 gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt
    26221 tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata tgcatatgct
    26281 gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc
    26341 gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg
    26401 ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc acatacacgg
    26461 gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga aattcaccac
    26521 gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg cagcggtgaa
    26581 aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg tgacctggtg
    26641 ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg caccttcccg
    26701 gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct ggagtctctc
    26761 gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac tggtgaagtg
    26821 gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa tgttgatgaa
    26881 ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttagga
    26941 ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct ggaaccgggg
    27001 atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt gccagaacaa
    27061 tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac cggcaacgaa
    27121 aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg
    27181 agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg
    27241 ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg actgcgccag
    27301 agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg ggtacctgtc
    27361 agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc gccatcacta
    27421 ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca gaagattacc
    27481 aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat
    27541 tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct aacgttgccc
    27601 gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg ggccgcgtgc
    27661 tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt gattggcagc
    27721 aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt gcgcacgaag
    27781 gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg atgtctgacg
    27841 gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga
    27901 gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg gggcaaatta
    27961 cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc agaccgatta
    28021 cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag
    28081 ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact caggcgcatg
    28141 agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag cgtcgacaga
    28201 gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt gccaaccgtt
    28261 gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta ttcaccccgg
    28321 cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg cggttttaag
    28381 gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt ggggctggcg
    28441 gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca gcgcgtaccg
    28501 gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa tgccgccagc
    28561 gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag ggccagttgt
    28621 tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt tgacgatcaa
    28681 agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca ctacgcgctg
    28741 tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga attacagcag
    28801 gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat gttaacggcg
    28861 cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc agatattccg
    28921 ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg
    28981 catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt tttaccgctt
    29041 agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc gcagcggatg
    29101 cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga taatcgcctt
    29161 ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg ttatgcgcgc
    29221 cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat tcacccactg
    29281 gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc tgaactgaaa
    29341 cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg ctatgaacgt
    29401 agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg cgatctcttt
    29461 tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact ggccgacacg
    29521 cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga tttgcctgaa
    29581 tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac tttcatctcc
    29641 cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc tccgttttcg
    29701 cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt tttttagctc
    29761 ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata gaaattggtg
    29821 tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa gctaatgtga
    29881 tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg caaaagagaa
    29941 cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat aatttcacca
    30001 tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca gactcctttg
    30061 tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg gatgatgccg
    30121 ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc cggaatgttt
    30181 gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga gtttctgcgt
    30241 tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct ttactcgggg
    30301 atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt tactgaagat
    30361 ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg gattacccaa
    30421 cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat gattgccgtg
    30481 caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca gcgtcaggcg
    30541 gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat tgccaccact
    30601 ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc ggccgatttc
    30661 tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg tgacacgctg
    30721 cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat ttctccttta
    30781 gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt tatcggtcgt
    30841 gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct ggtgatgacc
    30901 gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca gggcaaccag
    30961 catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag cattgcgctg
    31021 gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg
    31081 ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc gtgatttact
    31141 tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc aaagaacacg
    31201 aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa catgctcagg
    31261 agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg
    31321 gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat gcgccagtaa
    31381 gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg gtgaacagcg
    31441 aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc gaactggaat
    31501 cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg ctttattcct
    31561 cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact ctcttcgtaa
    31621 ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac acagacgtta
    31681 agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga cgccgcgcaa
    31741 cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac cggccagatt
    31801 gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc gaccgaatac
    31861 gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac gtcttacatc
    31921 ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat gctggaaaat
    31981 ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg ccgccttgaa
    32041 gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat ggcctctgct
    32101 tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc
    32161 aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca aacgctggat
    32221 gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa
    32281 gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac taccggtgaa
    32341 attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt
    32401 gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt
    32461 gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc acacgcggca
    32521 ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat cggtgtatcg
    32581 aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga gcaacatatt
    32641 cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc aaacatcgcc
    32701 agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa ccgcattcac
    32761 cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact gcgccatgcg
    32821 cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact gacgcgtgcc
    32881 gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat cacccttgat
    32941 gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg cgataaccac
    33001 gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc tatccagcct
    33061 gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta tcacagcgaa
    33121 accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct gaatcaggcg
    33181 atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat gatcccaatc
    33241 acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc tgaaggttat
    33301 cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt
    33361 tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc gattcgtcat
    33421 tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc ttctgcgcac
    33481 ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt ggcgtgtgat
    33541 aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc gggcgataac
    33601 ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga aacgatccgt
    33661 gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg cgcgaacatg
    33721 aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc gcacctcaac
    33781 ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg accgatcggt
    33841 gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg
    33901 ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat cctgccaatc
    33961 agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag ccaggtggca
    34021 atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt gctgtatacc
    34081 ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct gaaagaagaa
    34141 accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt ccacgcgccg
    34201 acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc tgaaagcaaa
    34261 gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg
    34321 aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca cattcagagc
    34381 gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt cccggcaggc
    34441 gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg cgaccgtaac
    34501 ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc gagtattttc
    34561 taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct cagacagtcc
    34621 cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg gcgcgaatat
    34681 ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc caacattgaa
    34741 gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt ttctcggaaa
    34801 aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg gcaactgcat
    34861 tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac ctccacgcgg
    34921 cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg ctccaggcgg
    34981 atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag cacgatgaac
    35041 cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc gttgaaaacc
    35101 ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat tttctctgct
    35161 gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc gctatcgtca
    35221 atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat tacgcggcat
    35281 cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga
    35341 tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc agcggcggcg
    35401 agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt ggacaggcag
    35461 aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac gtcacgggaa
    35521 gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg gatgcaacaa
    35581 gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca atcacctctt
    35641 tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt
    35701 tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat gacacgcagt
    35761 cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc actgcttttt
    35821 tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg atgctgccat
    35881 cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc ggcctctgat
    35941 aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc tgccagcccc
    36001 agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag atcgcatcgc
    36061 cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca agataatcgc
    36121 aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta aatccgcgac
    36181 gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca tcggtaaaga
    36241 cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta catgagtagg
    36301 gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta atgcgacgcg
    36361 cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg tacatcgtct
    36421 cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca aacagcggcg
    36481 cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg actttatgct
    36541 tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat ttacggttgg
    36601 tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag agggttatca
    36661 ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac ttcagccctt
    36721 cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg atggaagtcc
    36781 gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag tgaccataaa
    36841 aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg gtgatttcgc
    36901 gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct ttgccgcctt
    36961 tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag aaatctttcg
    37021 gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac aggaaatagt
    37081 accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt ctggctgggc
    37141 gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt gatgtgcttc
    37201 gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc gcaacctcaa
    37261 ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg accctgcctg
    37321 aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat atgattgaat
    37381 ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt aatttcatga
    37441 tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt cactggaact
    37501 attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga gtaagttatg
    37561 gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc caacagcgtc
    37621 agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact ggttcaggcg
    37681 gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg cggcagtatg
    37741 atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg ggcaaaaatg
    37801 tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac ctacacgccg
    37861 tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt tatctggagc
    37921 ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa aattttatct
    37981 ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga aatggcagtt
    38041 aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta ttcgctcggg
    38101 gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca cggcttcgtg
    38161 ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca ggcgtaatgg
    38221 gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg gagcgcaacc
    38281 gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt gtgataaagg
    38341 catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa atgcctgatg
    38401 cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca gtgtattcgc
    38461 atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga cgacctgtac
    38521 cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac gacgccagtt
    38581 ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg cactacctgc
    38641 cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca ccatctcttg
    38701 tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat tggtcgcctg
    38761 tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt caatgtttaa
    38821 tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat cggtcagcat
    38881 attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt caaaccatag
    38941 caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt cgctaaagag
    39001 atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac gctcgtcgtc
    39061 tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca gctgcggttc
    39121 acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca gacgtaaatt
    39181 gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat cttctgccat
    39241 ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg cgccagtaat
    39301 ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg cagaagccgt
    39361 aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg cttaacggcg
    39421 tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac tacgcgatct
    39481 gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt gaattaattt
    39541 cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac acgttacaat
    39601 agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac aaagcccgaa
    39661 ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg tcgtttttcg
    39721 aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt ctgctggaat
    39781 gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc gatgcagaac
    39841 tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct gtggcaatct
    39901 gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg gctggttgcc
    39961 gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat ggtgttactt
    40021 tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg ccagggggaa
    40081 attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag catcgtcaaa
    40141 gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg cggtaaacgg
    40201 caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga tttacggcgg
    40261 attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg cgctaagccg
    40321 tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc accagctctg
    40381 aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac ccacgaacag
    40441 ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg ttggccagta
    40501 gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga gcgcatcgag
    40561 gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa ttttaccttc
    40621 aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga tttcaccaaa
    40681 atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt tcggggagtc
    40741 gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga ttttttctgc
    40801 cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca taacgtaagc
    40861 ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag tcacggcaca
    40921 ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca ggagctggca
    40981 cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga aaatacgttg
    41041 aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg cgggttgacg
    41101 ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct ggagggcggg
    41161 tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt ccagtatctt
    41221 tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc gaaattgccc
    41281 cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca ggcgccatta
    41341 attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta tgctaccggg
    41401 ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg tcagggcgta
    41461 gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga agaggcggtt
    41521 tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg tgtgtcaatt
    41581 gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt ctggcaccgc
    41641 attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc gcatgtgttg
    41701 cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt ggtgcagatg
    41761 ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc taccgagcgt
    41821 ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag aacgggaaga
    41881 tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg ctttgctcag
    41941 gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag cagcgatatt
    42001 cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt gttgtacatc
    42061 accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg cacggctccg
    42121 gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa agagatgatc
    42181 gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat tacctgtggt
    42241 tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat caccgtgcgt
    42301 tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat gaaagctatc
    42361 tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa aagcgtcgca
    42421 ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca gcttggcgtt
    42481 agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta ccagccgccg
    42541 aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa ataattcgcg
    42601 tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac ttgcccgctg
    42661 aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt gcgcaagaac
    42721 tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc gaaaaggccg
    42781 ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc ggtgatttcg
    42841 acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg cttggttgca
    42901 gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta agcccggaag
    42961 tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat aacggtattt
    43021 cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt gttaccgatc
    43081 accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct aacttgcgcg
    43141 actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat ctgatgctgg
    43201 cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc gcaattccta
    43261 acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc gtgccgctgg
    43321 acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc ggaaagtgcc
    43381 gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa ctagccgcca
    43441 gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg gatgatatgt
    43501 ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg ctggcaaatg
    43561 aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag gttgaagccc
    43621 tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg ctggcaatgt
    43681 atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc aaagagcgtt
    43741 ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa ggttccggtc
    43801 gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca ctctaccccg
    43861 gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg gaagaggata
    43921 aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg gccccttcgc
    43981 tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg accatggaag
    44041 tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag ccgctgtttg
    44101 acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg aaggtgatgg
    44161 tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc gataccgctc
    44221 tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat atcaacgagt
    44281 ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt tagcgtcatc
    44341 ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg cattttgact
    44401 agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat cgcattcagg
    44461 acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc caagaaagag
    44521 cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga acccgaacgc
    44581 gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac cctcgaccaa
    44641 atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga agctgacgac
    44701 gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa actggcgcag
    44761 cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta cctcgatatt
    44821 caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga gcgtatgtat
    44881 ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc ggaaggtgaa
    44941 gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta cggctggctg
    45001 cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc cggcggtcgt
    45061 cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga tattgatatc
    45121 gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc gggcggtcag
    45181 cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg gatcgtgacc
    45241 cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa gcagatgaaa
    45301 gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc gatggaagat
    45361 aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga tgactcccgc
    45421 attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct ggacggcagc
    45481 ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa catgtctgaa
    45541 caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa aacgcgtcgt
    45601 gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt ccgtcgcgat
    45661 catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga actggaagcg
    45721 ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat gggtaaagcg
    45781 tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc ccgtgacgat
    45841 ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga catcctcggc
    45901 gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg taccgagctg
    45961 cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt gcaggatcag
    46021 gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc ccgtaacacc
    46081 tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa ccgcggcttt
    46141 atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc gcgtccgttt
    46201 atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc ggaactgtac
    46261 ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg taacttccgt
    46321 aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact ctacatggct
    46381 tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac tctggcacag
    46441 gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt cggtaaaccg
    46501 ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac cgacatggcg
    46561 gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat ccacgttgag
    46621 aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc agaagcacat
    46681 ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct ggcgcgtcgt
    46741 aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg tcgtgaaatc
    46801 ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt cctggatcag
    46861 gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga ttacgttacc
    46921 gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga ccgtatggta
    46981 atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc gatgcgtccg
    47041 gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt tttatttaag
    47101 ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca tcaccatcca
    47161 cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta aatcacacca
    47221 ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc gtgcggcaaa
    47281 taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt agcgaaagtc
    47341 aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc aacggcggat
    47401 caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt tagtccacac
    47461 gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc ccttggcatt
    47521 aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg cggcatactt
    47581 ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt ccgtttgcat
    47641 aatttctcac atgtaattct gatcactcat cattatagat aattgaagag tgaaatgatt
    47701 aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca ggtaaaaacc
    47761 atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc aacacagtca
    47821 gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc gcgcattaaa
    47881 aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa gccacatacg
    47941 tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt cagcatgatg
    48001 ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga gcataatgcc
    48061 gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt gagagagcgt
    48121 cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac ggttggtggt
    48181 gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga acatcactag
    48241 accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca tttttggcac
    48301 catgccaaac aggattaata taattcccga agaaatacac acccagcggc tatgaacgcg
    48361 catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc tgttaaacgt
    48421 gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat cgtgtgaaga
    48481 gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact cgataaacac
    48541 gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg gcataccaaa
    48601 cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata aattgacttc
    48661 attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg cgacgttgga
    48721 cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg caaaggagat
    48781 acctaaataa acgggattac catattgcgg attcccttta cctccagcgg cccagtcaat
    48841 acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa ccagtggcgg
    48901 gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa aacccgcggc
    48961 gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa tggctatcat
    49021 tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc ccataaagcg
    49081 gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat ccgagctaat
    49141 gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa gaggaacagc
    49201 gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca gctttcctgg
    49261 tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg agggaagttg
    49321 ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa agcaaccgtt
    49381 tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc gaggaagggg
    49441 agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac gaaaatgaca
    49501 agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca ttaatcattc
    49561 tggcatgtcg cactctcgca tttaatcgtt tttat
//
LOCUS       DAJTRW010000020        49533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRW010000020 DAJTRW010000000
VERSION     DAJTRW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1152)
                     /gene="setB"
                     /locus_tag="OO326_001263"
     CDS             complement(<1..1152)
                     /gene="setB"
                     /locus_tag="OO326_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5965712.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1520..2650
                     /gene="fruB"
                     /locus_tag="OO326_001264"
     CDS             1520..2650
                     /gene="fruB"
                     /locus_tag="OO326_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5965713.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2650..3588
                     /gene="fruK"
                     /locus_tag="OO326_001265"
     CDS             2650..3588
                     /gene="fruK"
                     /locus_tag="OO326_001265"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5965714.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3605..5296
                     /gene="fruA"
                     /locus_tag="OO326_001266"
     CDS             3605..5296
                     /gene="fruA"
                     /locus_tag="OO326_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5965715.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5720..6661
                     /locus_tag="OO326_001267"
     CDS             5720..6661
                     /locus_tag="OO326_001267"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5965716.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6649..7587
                     /gene="psuG"
                     /locus_tag="OO326_001268"
     CDS             6649..7587
                     /gene="psuG"
                     /locus_tag="OO326_001268"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5965717.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7681..8931
                     /locus_tag="OO326_001269"
     CDS             7681..8931
                     /locus_tag="OO326_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5965718.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9002..9696)
                     /gene="yeiL"
                     /locus_tag="OO326_001270"
                     /pseudo
     CDS             complement(9002..9696)
                     /gene="yeiL"
                     /locus_tag="OO326_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9827..10768
                     /gene="rihB"
                     /locus_tag="OO326_001271"
     CDS             9827..10768
                     /gene="rihB"
                     /locus_tag="OO326_001271"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5965719.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10868..12118
                     /locus_tag="OO326_001272"
     CDS             10868..12118
                     /locus_tag="OO326_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5965720.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12174..13262)
                     /locus_tag="OO326_001273"
     CDS             complement(12174..13262)
                     /locus_tag="OO326_001273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5965721.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="OO326_001274"
     CDS             complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="OO326_001274"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5965722.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14196..15245)
                     /locus_tag="OO326_001275"
     CDS             complement(14196..15245)
                     /locus_tag="OO326_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5965723.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15344..16225
                     /locus_tag="OO326_001276"
     CDS             15344..16225
                     /locus_tag="OO326_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5965724.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16431..17900
                     /gene="lysP"
                     /locus_tag="OO326_001277"
     CDS             16431..17900
                     /gene="lysP"
                     /locus_tag="OO326_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5965725.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18192..20183
                     /gene="cirA"
                     /locus_tag="OO326_001278"
                     /pseudo
     CDS             18192..20183
                     /gene="cirA"
                     /locus_tag="OO326_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20215..21050)
                     /gene="yeiG"
                     /locus_tag="OO326_001279"
                     /pseudo
     CDS             complement(20215..21050)
                     /gene="yeiG"
                     /locus_tag="OO326_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21308..21976
                     /gene="folE"
                     /locus_tag="OO326_001280"
     CDS             21308..21976
                     /gene="folE"
                     /locus_tag="OO326_001280"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5965726.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21993..23069
                     /locus_tag="OO326_001281"
     CDS             21993..23069
                     /locus_tag="OO326_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5965727.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23211..24251
                     /gene="galS"
                     /locus_tag="OO326_001282"
     CDS             23211..24251
                     /gene="galS"
                     /locus_tag="OO326_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5965728.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24531..25529
                     /gene="mglB"
                     /locus_tag="OO326_001283"
     CDS             24531..25529
                     /gene="mglB"
                     /locus_tag="OO326_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5965729.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25590..27110
                     /gene="mglA"
                     /locus_tag="OO326_001284"
     CDS             25590..27110
                     /gene="mglA"
                     /locus_tag="OO326_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5965730.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27126..28136
                     /gene="mglC"
                     /locus_tag="OO326_001285"
     CDS             27126..28136
                     /gene="mglC"
                     /locus_tag="OO326_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5965731.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28172..29385)
                     /gene="preA"
                     /locus_tag="OO326_001286"
                     /pseudo
     CDS             complement(28172..29385)
                     /gene="preA"
                     /locus_tag="OO326_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29379..30617)
                     /gene="preT"
                     /locus_tag="OO326_001287"
     CDS             complement(29379..30617)
                     /gene="preT"
                     /locus_tag="OO326_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5965732.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30811..31050)
                     /locus_tag="OO326_001288"
     CDS             complement(30811..31050)
                     /locus_tag="OO326_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5965733.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31053..31772)
                     /gene="sanA"
                     /locus_tag="OO326_001289"
     CDS             complement(31053..31772)
                     /gene="sanA"
                     /locus_tag="OO326_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5965734.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31922..32806)
                     /gene="cdd"
                     /locus_tag="OO326_001290"
     CDS             complement(31922..32806)
                     /gene="cdd"
                     /locus_tag="OO326_001290"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5965735.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32936..33631)
                     /locus_tag="OO326_001291"
     CDS             complement(32936..33631)
                     /locus_tag="OO326_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5965736.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33628..34026)
                     /locus_tag="OO326_001292"
     CDS             complement(33628..34026)
                     /locus_tag="OO326_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5965737.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34265..35212
                     /gene="dusC"
                     /locus_tag="OO326_001293"
     CDS             34265..35212
                     /gene="dusC"
                     /locus_tag="OO326_001293"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5965738.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35583..35666)
                     /gene="yohP"
                     /locus_tag="OO326_001294"
     CDS             complement(35583..35666)
                     /gene="yohP"
                     /locus_tag="OO326_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5965739.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35890..37326
                     /gene="mdtQ"
                     /locus_tag="OO326_001295"
                     /pseudo
     CDS             35890..37326
                     /gene="mdtQ"
                     /locus_tag="OO326_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37379..38140
                     /locus_tag="OO326_001296"
                     /pseudo
     CDS             37379..38140
                     /locus_tag="OO326_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38257..38835)
                     /locus_tag="OO326_001297"
     CDS             complement(38257..38835)
                     /locus_tag="OO326_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5965740.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39005..39592
                     /locus_tag="OO326_001298"
     CDS             39005..39592
                     /locus_tag="OO326_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5965741.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39766..40698
                     /gene="pbpG"
                     /locus_tag="OO326_001299"
     CDS             39766..40698
                     /gene="pbpG"
                     /locus_tag="OO326_001299"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5965742.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40736..42451)
                     /gene="dld"
                     /locus_tag="OO326_001300"
     CDS             complement(40736..42451)
                     /gene="dld"
                     /locus_tag="OO326_001300"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5965743.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42647..44944
                     /gene="bglX"
                     /locus_tag="OO326_001301"
     CDS             42647..44944
                     /gene="bglX"
                     /locus_tag="OO326_001301"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5965744.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45196..46113
                     /gene="osmF"
                     /locus_tag="OO326_001302"
     CDS             45196..46113
                     /gene="osmF"
                     /locus_tag="OO326_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5965745.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46120..47277
                     /gene="yehY"
                     /locus_tag="OO326_001303"
     CDS             46120..47277
                     /gene="yehY"
                     /locus_tag="OO326_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5965746.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47270..48196
                     /gene="yehX"
                     /locus_tag="OO326_001304"
     CDS             47270..48196
                     /gene="yehX"
                     /locus_tag="OO326_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5965747.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48201..48932
                     /gene="yehW"
                     /locus_tag="OO326_001305"
     CDS             48201..48932
                     /gene="yehW"
                     /locus_tag="OO326_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5965748.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48913..49020)
                     /locus_tag="OO326_001306"
     CDS             complement(48913..49020)
                     /locus_tag="OO326_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5965749.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49080..49533)
                     /locus_tag="OO326_001307"
                     /pseudo
     CDS             complement(49080..49533)
                     /locus_tag="OO326_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11957 a        12206 c        13310 g        12060 t
ORIGIN      
        1 cagagtggcg ataatcatca ccatcgcaaa ccagaacaca gcgtgataat tccagatctc
       61 ggcgacgatg cccgccactg atcctgcgat gatccagccc acgcgcgacg tgttggtata
      121 gagcgtggtg gctgaacctg cctgaccggg catcaaatcc tgaaaataga gcatcccgat
      181 gccgcccaga atgccaataa aaatagcatt tagcagctgc aagcccaaca gaatggcagg
      241 tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca cccacggcag caacgcgcat
      301 taagaaacgc ttacccagac gtttggcgaa atatccggca atcaacatcg tcgggatttc
      361 cagcccggcg gcggtcccca tcatcacacc ggccagtttc tcgggaagat gcagttcgtt
      421 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
      481 aatgacaaac agcagtagcg tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt
      541 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca cgccgcaaac
      601 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaacgc
      661 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
      721 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
      781 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
      841 gttgcgattc caagcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
      901 cagcgatttg cgatcgccgc gcttatcaga gcgcccagcg agaaactgac ttaccagaat
      961 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacggg catgtacttc
     1021 atcggtaagg aaaatactga gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa
     1081 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     1141 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     1201 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     1261 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     1321 caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     1381 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     1441 tgtctttaac cctaagctag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     1501 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     1561 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     1621 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     1681 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     1741 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     1801 cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac gccagctgac
     1861 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga
     1921 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     1981 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     2041 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     2101 gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc
     2161 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     2221 ggtgagtgtg gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt
     2281 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     2341 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     2401 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     2461 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     2521 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     2581 tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga
     2641 gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta tgaccttgtt
     2701 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     2761 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     2821 gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     2881 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     2941 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt tcgaagtcac ccccgccgac
     3001 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     3061 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     3121 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     3181 aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt
     3241 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     3301 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     3361 tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     3421 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     3481 gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     3541 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     3601 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc
     3661 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     3721 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     3781 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     3841 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     3901 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     3961 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     4021 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     4081 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     4141 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac tggataaagc
     4201 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga ccactgaagg
     4261 taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt acatgctgcc
     4321 gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     4381 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     4441 gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc gtccgggcct
     4501 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     4561 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     4621 gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     4681 ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc tcgaagggct
     4741 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     4801 tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt
     4861 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     4921 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     4981 gcaggaaggt ggcaaagccg ctctggtatt gggactgtgc ttcatttcgg aaggtgcaat
     5041 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     5101 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     5161 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     5221 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     5281 agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt
     5341 tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt aaatggtttt
     5401 atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct caagggcaga
     5461 ttattttctg ccctttttta gtgttcctgt tctgtgcatc ccgtcacaaa ttcctcgctt
     5521 ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc attttgcctc
     5581 tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     5641 cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     5701 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     5761 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     5821 aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg cgttgctggg
     5881 taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac
     5941 gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     6001 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     6061 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
     6121 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
     6181 taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
     6241 gcgcgaccgt ctaaatcaga accacactct gaagccaaac cgccttgaag cggaaaccct
     6301 gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca
     6361 acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt acagcgatat
     6421 cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg ttaccggagc
     6481 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
     6541 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
     6601 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
     6661 aaaatttccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa
     6721 aaaccggttg tggcgctgga atcgaccatt atttctcacg ggatgccgtt cccacaaaat
     6781 gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt acctgccact
     6841 atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat tgaattactg
     6901 ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
     6961 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
     7021 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
     7081 gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg
     7141 gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt cggtgtgccg
     7201 ttaattggct atcagactaa agcgctgcct gcgtttttct gccgtaccag ctcgtttgac
     7261 gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt gaaatggcaa
     7321 agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg
     7381 gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga gcagggcgtt
     7441 attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac cagcggtgac
     7501 agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag tgaaattgcc
     7561 aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc
     7621 atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
     7681 atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg
     7741 tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct gctgcaaatc
     7801 gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga acaggcggca
     7861 ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg
     7921 ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc
     7981 gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg
     8041 atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa
     8101 atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc
     8161 gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg
     8221 atggcgtcca ttgctggttc ggtgatgatt ggttatgccg gaatgggcgt accaattgac
     8281 tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc acgtattctt
     8341 agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg
     8401 ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct
     8461 ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc
     8521 ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat
     8581 gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa ccttgctggt
     8641 agcctgattg ggcagaaact ggcgattaac gaattcgtcg cttacctgag tttctcccca
     8701 tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt
     8761 tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
     8821 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
     8881 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctcgatt
     8941 tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt gcaaataatt
     9001 tttactgcat catcccggag aatttattct ccgggtccat ctccagcgcc agtcctgaca
     9061 actgctttct gtttttaatt agatatcctt tcttttaatt aataaaccgt cgtgaatgaa
     9121 ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg ccgcttgcgt
     9181 atgcttttcg tgataaagat caccttcttg tgagagtaat ataaatgctg ccaggcgatt
     9241 cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat ttttatgact
     9301 taaggtgacg caaagttttc gtaaaaatag ggtgtcgttt aataacagcg gacggtaatg
     9361 tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac gcggttcatg
     9421 gtctttgtcg attaactcaa tctcgccaat aaaacaggga gcggcaaaga aatcgatcag
     9481 cgacacgcga ccattggcaa gcgtggcgta aagcctggcg cggcctcgcg tcagataaaa
     9541 cagccatgac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag
     9601 ccgcgtatcg gctgaaacat ccgttgaaaa gcaatcctta aacgcagatt cactcatcaa
     9661 ttgctgctta aggtcattat tgtggatttc tttcacgatg tttatccgct atgagatttc
     9721 tcatatttag catacgtacg ctgttgttaa gattcaatca ttggcccggt cgaattttct
     9781 ttgctgtaga aaaaaataac gcaactggaa acagaggaaa taaacaatgg aaaagagaaa
     9841 aattattctg gattgtgagc cgggtcatga tgatgctatt gcaatgatga tggcagcgaa
     9901 acatccggca atagatttat taggcatcac tattgtagcg ggtaatcaga cgcttgataa
     9961 aacattaatt aatggcctga atgtttgcca gaaactggag attaatgttc cggtttatgc
    10021 ggggatgccg caacccatta tgcgtaaaca aatcgttgcc gataatattc acggtgaaac
    10081 cggactggat ggcccggtat tcgagccgct gacccgccag gcagaaagta cccatgcggt
    10141 gaaatatatc atcgataccc tgatggcaag cgatggcgat atcactctgg tgccggttgg
    10201 tccgctttca aatatcgcgg tggcaatgcg tatgcaaccc gcaatcctgc ccaaaatccg
    10261 tgaaattgtg ctaatgggcg gcgcttacgg tacaggcaac ttcaccccat ctgccgagtt
    10321 caatatcttt gccgacccgg aagccgcccg cgtggtgttc acctccggcg ttccattagt
    10381 gatgatgggc ctcgatctta ccaaccagac cgtttgcacc ccggatgtaa ttgctcggat
    10441 ggaaagggta ggcggccctg ccggagagct gttcagcgac atcatgaact tcactctcaa
    10501 aacgcagttc gaatactacg gcctggctgg cggcccggtg catgacgcca cctgcattgg
    10561 ttatctgatc aaccctgatg gcattaaaac ccaggatatg tacgtcgaag tggacgtcaa
    10621 cagcggccct tgctacgggc gtacggtctg cgacgaactg ggcgttcttg gcaagcccgc
    10681 taataccaaa gtcggcatca ctattgatac cgactggttc tggggcttag tcgaagagtg
    10741 cgtgcgcggc tacatcaaaa cccattaagt gttgataaca agccgggaca gtatcgcaag
    10801 atgcaggccc ggctatttct gatgggcaga aagccgtcgc ccaaaatgac atgaagagat
    10861 aatcactatg gatgtcatga gaagtgttct gggaatggtg gtactgctgg caattgcgtt
    10921 tttactgtca gtaaacaaga agaagatcag cctgcgtacc gttggcgcgg cgttagtgtt
    10981 acaggtcgtg attggcggga ttatgctttg gttaccgcca gggcgttggg tcgcagaaaa
    11041 agtcgctttt ggcgtccaca aagtgatggc gtacagcgac gcaggtagcg catttatctt
    11101 cggttctctg gtcggaccga aaatggatac cttatttgat ggcgcaggat ttatctttgg
    11161 tttcagggta ttaccggcaa ttatcttcgt cactgcactg gtgagtattc tctactacat
    11221 cggtgtgata gggatcttaa ttcgcattct cggcggtatc ttccagaaag ctttaaatat
    11281 cagcaagatc gagtcattcg tcgcggtcac caccattttc ctcgggcaaa acgaaattcc
    11341 ggcgatcgtc aaacccttta tcgatcgtct gaatcgcaat gaattattta cagcgatttg
    11401 tagtggcatg gcctcgattg ctggttcgac aatgattggt tacgccgcac tgggcgtacc
    11461 tgtggaatat ttgctggcgg catcgttaat ggcgatccct ggcgggatct tgtttgcccg
    11521 cctgttaagc ccggctacgg aatcttcgca ggtttctttt aataacctct ctttcaccga
    11581 aacaccgcca aaaagcatta ttgaagccga tgcgacaggg gcaatgaccg ggctgaaaat
    11641 cgccgccggt gtagcgacag ttgttatggc atttgtcgcc atcattgcgt taattaacgg
    11701 tattatcggc ggcgttggcg gctggtttgg ttttgaacat gcctcgctgg agtccatttt
    11761 aggttacctg ctggccccac ttgcgtgggt aatgggtgtt gactggagtg atgcaaatct
    11821 tgccgggagt ttgattggac agaaactggc aataaatgaa tttgtcgctt atctcaattt
    11881 ctcaccctat ctgcaaacgg ctggcactct ggatgctaaa accgtggcga ttatttcctt
    11941 cgcgttgtgc ggtttcgcta actttggttc tatcggggtg gtggtggggg cgttttctgc
    12001 ggttgcgcca caccgtgcgc cggaaatcgc ccagcttggt ttacgcgcgc tggcggcggc
    12061 gacactttct aatctgatga gtgccaccat tgccgggttc tttattggtt tagcgtagtg
    12121 tgcaatttat gagccggatt agatgcggtc aagcgtcgaa tccggcaaca ttgttacacc
    12181 attggcacta atgacagcgc gttatcggca gacagggtag ggttgtttaa gctgccgctg
    12241 gcgcgtgaaa ttgccgcgca ggccatcgca aaacgggcgc tgtcacggaa attgtttccc
    12301 tccagaaagc tatataccag acccgccata aaaccatcgt cagcaccaaa actgtctacc
    12361 gtcgtatgcg ctggcacagt cagcaaaaat tgttcaccat ccttttcgct gcaataaact
    12421 gactcatcgg gcaaataaac aaacagttgc tgaacacctt gctgatgcaa tgcattcact
    12481 gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc cagctccggt
    12541 aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc cgctttgaat
    12601 tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac ccattccagc
    12661 gcctcggctg tcaggttgca atcagccagt accacgcccg catgacgaag taaatcgcgc
    12721 gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt aattgccagc
    12781 acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg accatgcaaa
    12841 cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttccaggag catttcgcca
    12901 taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcctaacag cgccagattg
    12961 tgggcgatat tgcgtccaac gccgcctgcc gaacaatgaa ttgtaccggg atgagaagcc
    13021 gcttgcgggt aatggatatc cgccatcccg cgaatatcca tattgattgt ccccaccact
    13081 acgcagtatt cctgctcggt gagaatgtaa cctttgtctt taatccggcc tttgcgcatt
    13141 aaatccataa tatgcgccgc aacgcgcgaa cgactgattt gcagcatgtc cgcaatttcg
    13201 ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga tctccttttc ccggttattt
    13261 atcttcaggc taccgctttt tcagtttgtt gcgctttcag ccaggcgatc tcttctgccc
    13321 agatatccgg gttgatggtt tcaaggatca gcggaatgcc gtcgaaacgg tcgttctgca
    13381 taatccagcg gaacgcatca tgaccgatat tgccttcacc gaggctatga tggcggtcaa
    13441 cgcggctgcc aaaggtactt ttcgcatcgt taaggtgcat cccgcgcaaa tacttaaagc
    13501 cgacaatacg ggcaaaatca gcgaacgttt tttcgcattc ggctggagta cgcaaatcat
    13561 acccggcagc gaaagcatgg caggtatcaa tgcagacgcc gacgcgggat ttatcttcca
    13621 cgccgtcgat aatcgccgcg agatgttcga atttaaaccc taagttactg ccctgaccgg
    13681 cggtgttttc aatcaccgct gttacacctt gagttttatc cagcgcaatg ttgatggatt
    13741 cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat cagatggctg ccagggtgga
    13801 agttgagcaa agaaagcccc agttgttcgc aacgctgcat ttcatctata aaggcatcgc
    13861 gcgatttttc cagagcttca gtgaccggat gtccgaggtt gatcagataa ctgtcgtggg
    13921 gaagaatttg cgccgatgtg tagtgatatt tttcacaggc ggctttgaat tcatcgatgg
    13981 tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt tttggtgaac aaggcaaacg
    14041 cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc cagaccgcca gcagcactaa
    14101 cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa cccgctggag gaaaacggta
    14161 atgatagcgg gttaacagga gcaagaagta gtggtttatg cgatgacgct ttgaatcaca
    14221 tagttaatcg caccaccacc aacaatcagc caggcaaaca gtaccagtgc catcagcaga
    14281 ggtttcgccc cagctttttt cagcgcgctg acgtgagtgg tcagacccag cgccgccatc
    14341 gccattgcca gcaggaaggt atccagcgtt accagcatgt tcaccacgct ctgcggtaac
    14401 aggtggaacg agttaaagat ggcaactacg atgaacaaga tggcaaacca cggaatagtg
    14461 attttgcttt tctcgccgct gttcgcccca gacagctgtt taacacgcgc cgccagcagg
    14521 atgaggaacg gagccagcat catcacgcgc agcattttgg aaataactgc tgcgttttct
    14581 gcatccgggc tgatggcatg acctgccgcc accacctgcg ccacttcgtg cacagtagaa
    14641 ccaatgtaga taccgaaagt ttccggacta aaccattgag acatcagcgg atatatcgcc
    14701 gggtagagga aaatcgcgac ggtcccgaag ataacaacgg ttgcaacagc cacggttact
    14761 ttactggctt ccgctttcac taccggctca gtcgccagta ccgcggcagc accacagata
    14821 ctgctaccgg caccgatcaa ccaactggtg tgcttatcca gaccaaacac tttctgcccc
    14881 aggaagcaag ccagcaggaa ggtactggac agcgtcagca cgtcaatgat gatcccactg
    14941 ataccgacat cggcaatttg cgagaacgtc agacggaagc cataaagaat gatacccaga
    15001 cgtaataaat attgcttggc aaacagcaca ccaccgtcac agcttttcca gatgtgcgga
    15061 tagatggtgt tgcctaaaac catccccaac aagattgcga gggtgagggc actaaacccg
    15121 gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga ccccggtgat aactgcactc
    15181 agggctaacc ccggaataaa atgccacagt gtacgatgtt gtttctgtaa ggtgatattc
    15241 gtcataaccc tctcctttaa ccgggcataa ggttacgact aactggttta aaaataaaat
    15301 tgattatata tttataatta atctttataa gtggtaagcg actatgcaca tcaccctccg
    15361 gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg cgtcggtgat
    15421 gctggcgttg tcgcaatcag cagtgagcgc agccttgact gacctggaag ggcagcttgg
    15481 cgttcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg ggcggctgct
    15541 ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga
    15601 agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca ttctgcctgc
    15661 agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta gcgttgggaa
    15721 cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct ttattgaagg
    15781 accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc tggtggtttt
    15841 cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc tggccgctgc
    15901 gccgtggatc ctgcgtgaac gcggttccgg cacgcgggat attgtcgatt atctgttgct
    15961 gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg caatcaaaca
    16021 tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg aagatcaatt
    16081 gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg
    16141 gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc tggactattg
    16201 cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa catgctggtg ctgtgtcgat
    16261 ttcgtgacgc agcgccttca gcatgcattc gccagaaaag agattggctg ctttacttat
    16321 aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga aggattgcca
    16381 atcgtctgct acaatcgcgc catcattttt aagatggata gcatttttgt atgggttccg
    16441 aaactaaaac tacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga
    16501 tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc gcaacgattt
    16561 ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg atggtttact
    16621 tcctgatgac cagtctcggt gaattggctg catatatgcc ggtttccggt tcgtttgcca
    16681 cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg aactactggt
    16741 acaactgggc ggtgactatt gccgttgacc tggttgcagc acagctggtc atgagctggt
    16801 ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt atcttcctgt
    16861 tgaactacat ctctgttcgc ggctttggtg aagcggaata ctggttctca ctgatcaaag
    16921 tcacgacagt tattgtcttt atcatcgttg gtgtgctgat gattatcggt atcttcaaag
    16981 gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt gctggtggtt
    17041 ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga accgagctga
    17101 tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc gcggtacgtc
    17161 aggtattctg gcgaatcctg ttgttctatg tgttcgcgat cctgattatc agcctgatta
    17221 ttccgtatac cgatccgagc ctgctgcgta acgatgttaa agacatcagc gtcagtccgt
    17281 tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta
    17341 ttctgacggc ggtgctgtca gcaggtaact ccggtatgta tgcgtctact cgtatgctgt
    17401 acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaattgtcg cgtggtggcg
    17461 tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tttgtgcttc ctgacctcca
    17521 tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg acgggtttta
    17581 tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac gtattgcagg
    17641 gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg ccgatcttcg
    17701 cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc ctgaaagata
    17761 ccattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc ctgattattt
    17821 ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa atgaagttcc
    17881 cgcagaacga taagaaataa gtttcctccc ttccttgcta agccctctca accgagaggg
    17941 ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaaca tttggattga
    18001 taattgttat cgtttgcatt atcgttacga cgcaatcaaa aaaggctgac aaatcagagg
    18061 ctgttccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg atgctgccgt
    18121 acgcaagggg acgtaaagaa gatgtgagcg atacccattt tattttcgta gttacctcat
    18181 ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt ttgtccgcta
    18241 tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg cgaaacgatg gttgtcactg
    18301 catcttccgt ggaacaaaat cttaaagatg cacccgccag tatcagcgtc attacccagg
    18361 aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa gtgcctggcg
    18421 tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt ctggacagca
    18481 gctacaccct gattcttgtc gacggtaaac gcgttaactc ccgcaatgcc gtcttccgcc
    18541 acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt gaagtggttc
    18601 gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg cggtgtagtg aatatcatca
    18661 ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc attcaggaac
    18721 atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca ttaattgatg
    18781 gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat gacccgcaaa
    18841 actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc agccgcgacg
    18901 gcaatgtcga atttgcctag acaccgaatc aaaatcacga ttttactgcc ggatacggtt
    18961 tcgaccgtca ggatcgtgat tccgattcgc tggacaaaaa ccgcctggaa cgccagaact
    19021 actgcgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa tcctacggtg
    19081 agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa agcaatacgg
    19141 tcgacggcaa atacacgttg ccgctgacgg cgattaatca gtttctcacg gttggcggtg
    19201 aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc agctccaaaa
    19261 cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc gagccgctgg
    19321 cgctgacgac cggcgtgcgt atggacgatc acgaaaccta cggtgaacac tggagtccgc
    19381 gtgcctacat ggtttataac gccaccgaca ccgtaacggt gaaagggggc tgggcgacgg
    19441 catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat tcctgccgtg
    19501 gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa agttgggagc
    19561 tggggcttta ctacatgggt gaagaaggct ggctggaagg gggtgaatcc agcgttatcg
    19621 ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac gtcaacgctg
    19681 caccgggcta ccaaaacttt gttggttttg agacgggcgc taacggacgg cgcataccgg
    19741 tatttagcta ctacagcgtt aacaaagctc gtattcaggg cgtggaaacc gaactgaaaa
    19801 ttccgttcaa cgatgaatgg aaactgtcga tcaactacac ctacaacgat ggtcgtgatg
    19861 tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccatactgct aacggtacgc
    19921 tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt cactataccg
    19981 ggcagaaacg cgccgacagc gcgacggcta aaacaccggg cggttatacc atctggaata
    20041 ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg
    20101 gcgacaagga tctcagtcgt gacgactaca gctataacga agacggacgt cgttacttta
    20161 tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg actttcactt
    20221 cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga tgaagtagta
    20281 actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct ggcgcgcggc
    20341 ttctgccaat accgcaggtt gcaactggtc ggcaagaaac tgatcattat cgccctgatc
    20401 gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcgc aactatccca
    20461 ttccagcatg catttttgtc ctcacctaaa taggtgctaa acgctttgat tccccacggg
    20521 acgctgcacg gattcacaat gggcgcaaag gccgaaacgc tggtgtattt acccggattt
    20581 ttcagcgcca taatcagcgc accgtgacca cccattgagt gaccgctaat ggcgcagcgg
    20641 tcgctgacat taaattgcga ctgaaccagc gccggtaatt catcgcgcag ataatcatac
    20701 atccggtaat gcgtcgccca cggcggttgc gtggcattaa gataaaagcc tgcgccctgg
    20761 cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc gcgggctggt gtctggcatc
    20821 accagtacaa tccccagttc cgccgctacc cgctgggcac ccgccttggt ggtgaagttc
    20881 tcgtcattgc aggttaatcc ggaaagccag tacagcactg gtggcggggt gtgatcacga
    20941 ggggggggga gaaagatact gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc
    21001 cagcgttgct gccagccttc aaaacagcgg tgctcttcga gcatttccat gcgtggctcc
    21061 ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt caatgtgctg tgagtaactt
    21121 tcacttccgt atttgcataa cgatgtttta acatctgctg atgaaaggca gcggcaatta
    21181 caataattat cgctgtgaat actggattat gtgcgccgcc tcacgcacaa taatcaagct
    21241 gtaaatcagc ttaataactt tgcccccacg cagggtggag gcgtcacacc tgcaggagaa
    21301 atcataaatg ccatcactca gtaaagaagc ggccctggtt catgaagcgt tagttgcgag
    21361 aggactggaa acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag
    21421 ccttattgct ggtcatatga ccgaaatcat gcagctgctg aatctcgacc tggctgatga
    21481 cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg
    21541 tctggattac gccaatttcc cgaaaatcac cctcattgaa aacaaaatga aggtcgatga
    21601 aatggtcacc gtgcgcgata tcactctgac cagtacctgt gaacaccatt ttgttaccat
    21661 cgatggcaaa gcgacggtgg cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat
    21721 taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca
    21781 aattcttatt gcgctacaaa cgctgctggg caccaataac gtggctgtct cgatcgacgc
    21841 ggtgcattac tgcgtgaagg cgcgtggcat ccgcgatgca accagtgcca cgacaacgac
    21901 ctctcttggt ggattgttca aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt
    21961 gcgccatcac aactgattaa aaggcaggaa ccatggagcg caacgtcacg ctcgattttg
    22021 ttcgcggcgt cgccattctg gggatcctgc tattaaacat cagcgccttt gggctaccaa
    22081 aggcggctta tctcaatccc gcctggtacg atgctattac gccgcaggat gcatggacct
    22141 gggcatttct cgatctcatc ggccaggtga aattcctcac gctttttgcg ctgctgtttg
    22201 gtgctggcct gcaaatgttg ctgccccgtg gcagacgctg gatccagtcg cggttaacgc
    22261 tgttagtctt gttgggcttt attcacggtt tattgttctg ggacggcgat attctgctgg
    22321 cttacgggct ggtggggctt ggcgttttgc tgttattggg gctgatttcc gacagccaga
    22381 ccagccgcgc ctggacgccg gatgcatcgg ctattttgta tgaaaaatac tggaagcttc
    22441 acggcggcgt tgaagcgatc agtaatcgtg ccgatggtgt tggcaacagt ttactggcac
    22501 tgggcgcaca gtatggctgg caactggcag ggatgatgct cattggtgcg gcgctgatgc
    22561 gcagcggctg gctgaaaggg cagttcagct tacgtcacta tcgtcgtact ggttttgtgc
    22621 tggtggcgat tggggtgacc attaaccttc ctgccatcgc cctgcaatgg cagctggact
    22681 gggcgtatcg ctggtgtgcc ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc
    22741 aggcgattgg ctatgcgtcg ctgttttatg gcttctggcc gcaattgagc cgctttaagc
    22801 tggtgcttgc gatcgcctgc gtcggacgga tggcgctgac caactatcta ttgcaaacgc
    22861 tgatttgtac cacgcttttt taccacctcg gtttgtttat gcattttgac cgcctggagc
    22921 tgctggcgtt tgttattccg gtatggctgg cgaatatcct cttctctgtt atctggctgc
    22981 gttacttccg ccaggggccg gtggaatggc tctggcgtca gttaactttg cgtgctgccg
    23041 gaccggcaat atctaaaaca tcaagataac gatctggatt acattcgtta acaaaacggc
    23101 tgtaaccgtt tccattgctg tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa
    23161 tagccgccag caagcagtca tttactgcaa tctcataaca ggtagtgaat atgatcacca
    23221 ttcgtgatgt agcgcgtcag gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata
    23281 acagcacgct ggtcagtgcc gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg
    23341 attatcggcc aaacgccaat gcccaggcgc tggcaactca ggttagcgac accattggcg
    23401 tggtggtgat ggacgtttct gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg
    23461 tcgcccagca gcatcagaaa tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag
    23521 agcgtcacgc cattgaggtg ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa
    23581 aagcattgag tgacgatgaa ctggcgcaat ttatggataa cattcccggt atggtgttaa
    23641 tcaaccgcgt tgtgccgggg tacgcccatc gttgcgtttg cctggataat ctcagcggtg
    23701 cccgaatggc gacgcgcatg ttgctgaata acggtcatca acgtattggt tatctttctt
    23761 ccagccacgg cattgaagat gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag
    23821 agcaggatat tattccgccg gaaagctgga ttggcactgg tacgccggac atgccgggcg
    23881 gtgaggcggc gatggttgaa ctgctggggc gcaatctaca acttaccgct gtatttgctt
    23941 ataacgacaa tatggccgct ggcgcactga cagcattaaa agataatggc attgcgattc
    24001 cgttacatct ctcaatcatc ggtttcgatg atattcccat cgcccgttac accgacccgc
    24061 aattaacgac cgtgcgttat cccattgctt caatggctaa attagccacc gaactggcct
    24121 tgcagggggc agcaggcaat attgatcctc gtgccagcca ctgttttatg ccgacgttag
    24181 tgcgtcgcca ttctgtcgca acgcgccaga atgcggcggc gatcactaac tcaacaaatc
    24241 aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc
    24301 tatgattgca ccgttttaat gttgtaaccc gtatgtaaca gtgaataatc actttcaccg
    24361 aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag
    24421 aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg
    24481 ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc atgaataaga
    24541 aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct gcacacgctg
    24601 ctgatactcg cattggtgta acaatctata agtacgacga taactttatg tctgtagtgc
    24661 gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg atgaatgatt
    24721 ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg aaaggggtga
    24781 aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt gagaaagcgc
    24841 gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag gcgctggata
    24901 gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt attcagggcg
    24961 atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa gacggtcaga
    25021 ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca cgtaccactt
    25081 acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag ttagataccg
    25141 caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct ggcccgaacg
    25201 ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc gcggtagaag
    25261 cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag
    25321 cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt actgaacgat gctaacaacc
    25381 aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt gcggctgatg
    25441 gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc gtagataaag
    25501 acaacttggc tgaattcagc aagaaataag actgattggg cgcagtcgtt tactgcgccc
    25561 ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg tcttccgggg
    25621 aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag gcacttgata
    25681 acgttaattt aaaagtccgg ccacattcta tccatgcatt aatgggggaa aacggtgcag
    25741 gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc ggcaccattt
    25801 tattccaggg taaagagatc gatttccatt ccgccaaaga ggcactggaa aatggtattt
    25861 cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggat aacatgtggc
    25921 tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca
    25981 aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc ggcacattat
    26041 ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg
    26101 tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta
    26161 ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat aaaatggaag
    26221 aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg atcgccaccg
    26281 aaccgctggc aggactgacg atggacaaga tcatcgccat gatggttggg cgttctctta
    26341 atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc
    26401 taacgtcact gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga
    26461 tcctcggtat tgccgggctg gtgggggcga aacgtaccga tattgttgag acgttatttg
    26521 gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaacagatc aataaccata
    26581 atgccaacga agccataaac cacggatttg cactggtaac tgaggagcgc cgctcaacgg
    26641 gaatttatgc ctatctggat attggtttta actcgttaat ttccaatatt cgcaactaca
    26701 aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag tgggtgattg
    26761 attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc tccggtggta
    26821 atcagcaaaa ggtgattatt ggtcgctggc tactaacgca accagaaata ttaatgctcg
    26881 atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga aatttatcag ttaattgccg
    26941 aactggcgaa gaaaggcaag gggattatta ttatctcctc tgaaatgcct gagttgttag
    27001 ggataacaga ccgtattctg gtcatgagca atggtctcgt ttccggaatt gtcgatacaa
    27061 aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa gattaggggc
    27121 ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag ggcggtattt
    27181 acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca tttttaagtc
    27241 tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc gcgctcggtg
    27301 tggcagggtt aattgtcacc caggggaccg atctttctgc tggtcgtcag gtagggctgg
    27361 cggcagtggt ggctgcgaca ttattgcagt ccatggataa cgccaacaaa gtgttcccgg
    27421 aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga
    27481 tcggtttgat caacggtctg attatcgctt atctcaacgt gacgccgttc attaccacgc
    27541 tcggcacgat gatcatcgtc tatggcatca actcgctcta ttacgacttt gtcggggcgt
    27601 cgccaatttc tggttttgac agtggcttct ctacctttgc tcagggcttt gtcgcgctgg
    27661 ggagtttccg tctctcttac atcaccttct acgcgttgat tgcggtggcg ttcgtctggg
    27721 tgttgtggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt aacccggaag
    27781 cggcaaaaat atctggtgtc aacgtcggcc tgaacctgct gatgatctac gcgttgtctg
    27841 gcgtgttcta tgcctttggc gggatgttag aagccggacg tatcggctct gccaccaaca
    27901 acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt
    27961 tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg ggtaattatt tttaccgtca
    28021 tcaactatgg cctgacgtat atcggcgtaa acccatactg gcagtacatc atcaaagggg
    28081 cgattattat cttcgccgta gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg
    28141 attgcggata ttgccggatg cgtctctggt tttacaacgt taccgggtgt tctttctcgc
    28201 ctttcttaaa cttcacttcc ccgagctcaa tacaacccac cgggcagacg tgaccacaaa
    28261 gcagacaacc cacacatttc tcggtattac aatgcggtgt gcgggttttc tcgctccatt
    28321 ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt
    28381 tgtcaagatt gatacgggga tagacaatat aactgcggtc taaatcttcc gccgggacaa
    28441 tattgttatt cgccagacct accatctcct gcaacgaatc aaatccctga tcggcgagat
    28501 aatgcgataa cccgctcgcc atatcttcta cgatgcgata gccgtactgc ataatgccgg
    28561 tggtgacctg taacgttgct gcgccgagca ataaaaactc cgccgcatct tcccaggttt
    28621 caatgccgcc gataccgctg attgggaaat cgcgcagttc tggatgggtg cgcatttgct
    28681 gaatgaagcg cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt
    28741 taccgttaac gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa
    28801 cggtgttaat tgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc
    28861 acatatcacc gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccccgtttca
    28921 ctgcccgaca atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat
    28981 gagaagtcat ttgcggacag gagaagttac actcgatcat atccgcgcca gctgctccca
    29041 ttgctgctca ttttccccca tgatcgaagc gatcaatact ttgtccgggt aatcttcctt
    29101 cagccgacgc agggcggtca gattctcttc caacggatgt tcagcaatct gctccatatt
    29161 tttgaagccg ataaaaccgg tatcttcttt caccagatga tcaaaacgcg gcgagacttc
    29221 gttggcgata aaaaagccga tcgttttaaa caccacaccg ccccaacctg tgtcgtaggc
    29281 tttggcacac atctcatagc agttgcctac cggcgaagaa gagaggcaga acgggttggg
    29341 aaagttcacg ccgcaaaaag taatcgaaag atctttcgtt aacatgagca agctccctct
    29401 aaatagtgat gaatcgcctc ggcggcttct ttcccggttt tcacggcata gaccacggtt
    29461 ttgtcaccct caacaatatc gccagcagca aagacttgcg gatcgcggat ctgataattt
    29521 tgtgttttga tggtgttacg ctgctgctct aactccgcaa aggcatccag tctggcatgc
    29581 tgaccgacgg cgagaataat tttatctgcc gccatcgtca gttcgcctga taaccgtaca
    29641 tgcttaaacg tgactttatt gccttccacg gctactggcg tgaatccatc gatgatcgaa
    29701 acgcccagct cccgggcgct ggcaaactct ttttcgcttg ccggaaactc atctaactct
    29761 tcacgtgcta cgcaagttac cgcctgacag ccgagaactt tcagcgtgct ggctacgtcc
    29821 atcgcgacat caccgccgcc gataattaat gcgctttgtg gaatgctgat atcgccttgc
    29881 gcctgtcgta cacgttgcag gaagtcgacg gcaatctcaa cgtcgctatg ctcaaacagc
    29941 ggtagcccgg aaccgcttga taaccccacg gtgaccagta ccgcgcggtt ttccgctttc
    30001 agttgctcca gggtgagtgt tttaccgatt tcgttattgc acttaatggt cacgcccatt
    30061 ttttcaatac gggcgatctc tgcatccagc actgattgcg gtaaccggaa ttgcggaata
    30121 ccgttacgca accagccacc ggggtgcgct tctttctcat aaatcgtgac gtcataaccc
    30181 tggtttgtca gtgtcacact ggcctgcaat ccggcaggac ctgcgccaat aatcgcgact
    30241 ttgccgagcg ttttagtacc gggctgataa atttccatcc cggtttgttg ttcaaaatca
    30301 gtaacaaaac gctgtaagcg gccgatatca atgggagcat caacaccggc acgggtacaa
    30361 ccgctttggc ataatttctc cgtcgggcac actctggcac aaacggcacc gagggcatta
    30421 ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa agtagattga gcgaataaat
    30481 ttccccggat cggtctgtgc cgggcaagcc tgactacagg gagcgtcgtg acataataaa
    30541 cagcgagagg cttctttaat cgccagtaac ggcgtaaaag ccggagtgag ttcatccaga
    30601 taattttgtt gcggcatgtg atcttcctta atgctatgag taattatggc gaacgctaaa
    30661 taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttaagct gtgaaaggat
    30721 tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa gtaataatta
    30781 ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat aaacaatcat
    30841 gccgatagat aaaattccga agccaatata tgccaccata taagcgaaga agagtccggg
    30901 attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt tatcaatcgc
    30961 gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcgggataa ggaaaaaaac
    31021 ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt ttgtaattca
    31081 agtaactgtt cgggtgtaac ggcgggatac ccctgcgcat cttccggcac ctggtgcata
    31141 gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat ataaaggtca
    31201 gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat atctttcggt
    31261 gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa taatgctcgc
    31321 tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac tttgcgtgta
    31381 cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc tgatgggtcg
    31441 acaccagcag cgattaaatc tttgcgcatg gtcatcggct cgttataact ttgcaatgcg
    31501 ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt aatcgctcct
    31561 tgaatgcggt agcgataata ctgattaata acgccggtac gataatattt tgctgtgccg
    31621 agcaccacgc cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg
    31681 gttttccagc tcatccagcg atcgaggccg agcacagtca acagcagcaa gccgatcagg
    31741 actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgagctaa ggaaaatttc
    31801 catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt gccgggaatt
    31861 tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc cggcaccagg
    31921 cttaagcgag aagcacgcgg tcgatactgt gacagccgag agctttcagc gttgcggagg
    31981 tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat
    32041 ccgggtaatc ataacccttg agattcaaca gaattaacgc cccctgcaac ggcggcagag
    32101 tcgggttgaa tgcggcgttt tcagcgtagc tgccgctgaa aatacgaccg tctttacatt
    32161 ccagcgcgac accgcttggt gacttactgt aaggcatgtg cgaacggttt gccgctgcaa
    32221 tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc tcgtccatca
    32281 gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag tcacgcagcg
    32341 cgtgtgcctc gcggcccggc aaatgaatac gcagatccag accgctgttc agttcattca
    32401 taaactgacg gcagtgacca caaggcgtat agttaacggt gatggctgca agcgcttttt
    32461 caccactcaa ccaagcgtgg ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg
    32521 ttgcaccaat aaattccata ttggcaccaa aataccaggt tccgctcaca ccgcgcgcaa
    32581 ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgccgcc agcggaagta
    32641 gtgcgaatgc cagcgcgtct tcgtccagtc ccgttgcgct cttcagcgat gagacttgct
    32701 ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt gcagattgca
    32761 agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata
    32821 atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga tctgaatctc
    32881 attaattatg caacacgtga aacttacatc aaaaatgcgc gacgtatcgc aaattttagc
    32941 ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta attatcccgc
    33001 ataacaccag cgctagcgaa ctaaatgcac cttcctgata atccagctcg gcgcagcgcg
    33061 ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct ttggtacgaa
    33121 tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg acgaaaatca
    33181 cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc attgcaatag
    33241 gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg cccatcaaca
    33301 atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag atggtgatga
    33361 tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag gccagcgcga
    33421 cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtaa ctgtcgtaag
    33481 agatgccggt cagcattaaa aaaggaatga tcaccaccat tgctaccagc aacgggttaa
    33541 gcaggggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagaca atcaacgtca
    33601 gcggtaatga ccaccagata ttcgccatca ttcttctgat cctttctgac ctacgacttt
    33661 acgttcaccg tgtaccagtt gtgaactcca gctcaccacc agaaaaacca ccagcgtact
    33721 gacagcacag gaaaccacta ccgggccaaa ctgcgcgcgg agcaaatcaa aatattgcat
    33781 gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta cgtagcaccc
    33841 cggattgacc catttcgccg gaagaatttg caaggccagc aggacaaaca ggatcagcat
    33901 cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata
    33961 caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt tcagtgtctt
    34021 actcataatc aacaccccag atgagatgga ttcatcatac aattaattcg taaaacgtgc
    34081 taccgatcac attaagaaat cagcgcttat tttttcgcta cccggtatta cgcaatgagc
    34141 agcgaactca ggcatacatc aagcatagag agggattgca gtttgcagca atccctcttt
    34201 gcagaccttt aatccgctga ccataggcgc taacatagcg cctcattttt ttgcgggtga
    34261 tgatatgcgt gtgttactgg caccgatgga gggagtgctt gactctctgg tgcgtgaatt
    34321 gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg tggtggatca
    34381 actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ctacaaaacg ccagccggac
    34441 accatctggt acgctggtgc gcgtgcagtt gttaggtcag ttcccacaat ggctggcaga
    34501 gaacgccgcc cgtgcggtgg agttaggttt ctggggcgtg gatctcaatt gcggctgccc
    34561 gtcgaaaacg gttaacggta gcggcggcgg ggcgacgtta ctcaaagatc ctgaactcat
    34621 ctaccagggg gcaaaagcga tgcgtgaagc tgtaccggcg catttgcctg tcagcgtgaa
    34681 agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca
    34741 ggctggcgct acggagctgg tggtgcatgg gcggacgaaa gagcagggtt accgcgcgga
    34801 gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc cggtgattgc
    34861 caacggtgaa atctgggact ggcagagcgc gcaacaatgc atggcgatca gcggctgcga
    34921 cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg tggtaaaata
    34981 taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat atacccgtct
    35041 ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt ggttgagtta
    35101 tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg tgttgaataa
    35161 ctcccctgat attgcaaggg ctattcaggc aattgatatc gagaaactct aaaaaccata
    35221 gtgatgtttt tgacaatgtg ttagctcttt atcaataggc cgcacggttg tttggtggcg
    35281 tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg
    35341 cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagccgagg
    35401 gcaccgcgca gcggcgattt tgttcgccag agcccggggg ggcagggggc ggcggcgatt
    35461 ggtcgccccc tgcgcgctcc ttgcgccagt gattatatga agcaatgcac atgacaggag
    35521 cgcaacagga tgagagactg aaatattctc gttattttcg gatctgatga cattaattaa
    35581 ttttaaaata tcatcttaaa tacgccagtc accaccagta gcccaatcag gaaaataatc
    35641 aatacagccc agagtataat tttcattggc gttcctttat ttgtgagtgc tgaataagta
    35701 taggaaaaaa cgaagccctt tgccgttatc cataaacgat taagataaat cggaagatat
    35761 catttactta gtctatattt ttctgggtat gtcgttttat tgctactgat gtattgccac
    35821 gcgaaactaa aaaatatatc gcaacacacg gtaatctccg cgcaccgttt cttacctgat
    35881 tattccccca tgaatcgtga ttccttttat ccagccatcg cctgttttcc gctgttactg
    35941 atgctggccg ggtgtgcgcc tatgcatgaa acccgccagg cgttaagcca gcaaacgccc
    36001 gctgcacaag ttgacaccgc attacccacg gcgctgaaaa atggttggcc agacagccaa
    36061 tggtggctgg agtatcacga taatcagctc acttcattaa ttaacaatgc actacagaac
    36121 gcaccggata tgcaggtggc tgaacagcgc atttagctag ctgaagccca ggcgaaggca
    36181 gttgccactc aggatggtcc gcagatcgac ttttcggcgg atatggagcg gcaaaaaatg
    36241 tcggcagaag ggctaatggg gccgtttgct ctgaacgatc cggccgcagg tacgaccggt
    36301 ccgtggtaca ccaacggaac ttttggctta acggcgggct ggcatctcga tatctgggga
    36361 aagaatcggg cggaggttac tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc
    36421 gagcaaaccc gccaattgct ggctggcagc gtagcccgcc tgtactggga gtggcaaacc
    36481 caggcggcgt taaacacggt cttgcagcaa atagaaaaag agcagaacac cattatcgcg
    36541 accgatcgcc agctatatca gaacgggatt acttcttcag ttgacggtgt ggaaaccgat
    36601 attaatgcca gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat gaaaattatt
    36661 gaggcacggt taaatgcact cacaaatcac cagactaagt cattgaagct taaaccggtc
    36721 gcgttgccga aagtggcaag ccagcttcct gatgaactgg ggtactcctt actggcccgg
    36781 cgggcagatt tgcaggcggc gcactggtac gttgagtcat cgttaagcac tattgatgcg
    36841 gcaaaagcgg cattttatcc tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg
    36901 cacttaagcg atctgttccg tcattccgcg cagcaaatgg gcgttacggc aggcctgacg
    36961 ctacctattt tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc
    37021 aacttgtcta tcgccagcta caacaaagcg gtggttgaag cagtgaatga cgtggcgcgg
    37081 gcagccagtc aggttcagac actggcggag aaaaaccagc atcaggcgca aattgagcgc
    37141 gatgccttgc gtgtggtagg tcttgcgcag gcccgcttta acgcgggcat cattgctggt
    37201 tcccgcgtca gcgaagccag aatcccggcg ctgcgtgagc gggccaatgg cctgttattg
    37261 caagggcagt ggctggatgc ctccattcga ctcaccggtg cgctgggcgg gggttacaaa
    37321 cgctgatgat atgcgccttc tatacttaac gtttattcag cgttaagtgg agaactcgat
    37381 ggcataggtc gcgataatta ccgcttccga ttcggggatc ggcaaagagt gcgcgttatt
    37441 actggcgcag caggggtttg atattggcat tacctggcac tcagatgaag aaggggcaaa
    37501 agataccgcg cgtgaggtgg ttacccacgg cgtacgtgcg gagatcgtgc aactggatct
    37561 cggcaatctg ccagaaggtg cgcaggcgct ggagacactc atccagcgat ttgggcgtat
    37621 tgatgtgctg gtgaataatg cgggtgcaat gaccaaagcg ccgtttcttg atatggcttt
    37681 tgatgagtgg cgcaagattt ttaccgttga tgttgatggt gcatttttat gctcgcaaat
    37741 tgcggctcgt cagatggtga aataagggca gggcggtcgc atcatcaaca ttacgtcggt
    37801 acatgaacat acgccgctgc cggatgccag cgcctacaca gccgctaaac atgcgctcgg
    37861 cgggttaacc aaaacgatgg cgctggagtt ggtcaggcat aagattttgg tgaacgcagt
    37921 cgcgcctggg gcgatagcca cgccaatgaa cagcatggat gatagcgacg tgaagcccga
    37981 cgcggagcct tcgattcctc tacggcgttt tggcgcaacg catgagattg ccagcctggt
    38041 ggtgtggctt tgttcggagg gcgcaaatta cacgacaggg cagtcgttga tagtggatgg
    38101 cggctttatg ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg tgcgtggata
    38161 tttcaatggg ttaggccaga taagacacca gcgtcgcatc aggcatggaa cacccggtgc
    38221 cggatgtgga gtgaatgctt atctgtggca actgctttac gcctgattat ccggcttttt
    38281 cttcccgcgc cgtttcagcc accagcgcac gcccaccacc agaaccacga ccagaatcaa
    38341 ccagacccag tgcttcaaat gctggtcgag attgtgcaac cacggcgcaa tcacctgacc
    38401 accagcgtaa ccaatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattcag
    38461 cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg
    38521 aaagccatac ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataagttt
    38581 ctgtgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa
    38641 ccgccgcccg cacagataga gcacctggtc gccaatcatg ccaccaagcg ccacagaaag
    38701 taccaccagt gggaacttta atagcccctg atgtgccgca acgcctccca gcaaagtcac
    38761 ggtttcacct tccgccaggc taccgataac cagcgcggca taaccatatt gtgagatgag
    38821 tgtattgaga tccataggcc agtttgtctc cctttggttg cctgtaagca tacaccctgt
    38881 gaatcattca cgctgaaaaa ggacatacat cagacggtgc aaattacatg gatgaacaaa
    38941 aaataatcac aagctggctt atacttgaac tgtcaggttc atgaactcac aacaaggggg
    39001 cggtatgagc catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa
    39061 tcgcgaaaac gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat
    39121 tccggtgatt tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac
    39181 tatcctgaag ttatcctggt ttaccggact ggcgctggct gtcttgtttt acggcgttat
    39241 gctggctggg gttgcggtga tggggcgggt catctggtgg atggcgcgca attacccgca
    39301 gcgtccgtct cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt
    39361 aagcggtctg gtggcgcttt atccactggt ctggttgtgt gcgctggtcg gcacggtggc
    39421 gctgttttac accggctacc tgctttacct tggtattccg agcttcctga atatcaacaa
    39481 agaggaaggc cttagcttct ccagttcgac gctggcgatt ggcgtgctgg tactggaagt
    39541 gctgctcacc ctaaccgtta tactatgggg ctacggttat cgtttgttct agaaagttct
    39601 ttgacgttgc attgctggcg taaatgtcag caatgcagca tttactcacg attctccttt
    39661 gacgatcgca ctttttgctc gttatgatgg cgtcgctagc ctcagtaaat ccttactccg
    39721 gcggtgcgca acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc gaaatttcga
    39781 gtttctttat ttagcttggc cctgatgctg gctgtgcctt ttgcaccgca ggccgttgcg
    39841 aaaacggcag ccgctaccac cgcttcacaa ccggaaattg cctccggtag cgcgatgatt
    39901 gttgatctga ataccaacaa agtgatctat tcgaaccacc cggatctggt gcgtccgatt
    39961 gcgtctatca gcaaattaat gaccgcgatg gttgtgctgg atgcacgact gccgctggat
    40021 gaaaaactaa aagtggatat cagccagacg ccggagatga aaggggtcta ttcgcgcgta
    40081 cgactgaata gcgaaatcag ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa
    40141 aaccgcgcgg cggcaagcct tgcgcaccat tatcccggtg gttacaaagc ctttattaag
    40201 gcaatgaatg cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt tgaacctacc
    40261 ggattgtcgg tgcataacgt ttcaactgcc cgtgacttaa ccaaactgct cattgccagc
    40321 aaacaatatc cgttgatcgg gcagttaagt actacccggg aagatatggc aaccttctct
    40381 aacccgacgt atacgctgcc gttccgcaat actaatcatc tggtgtatcg cgataactgg
    40441 aatattcagt taaccaaaac cggctttacc aatgcggcgg gccattgtct ggtgatgcgt
    40501 acggttatca ataataaacc ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc
    40561 cattttgccg atgccagccg cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg
    40621 ccggcagcag cgttgagcta taaaaaacaa aaagccgccc aaatggcggc ggcggggcag
    40681 acggcacaga acgattaagt gaattcggat ggcgatactc tgccatccgt aatttttact
    40741 ccacttcctg ccagtttttc cgtttactgg ttttaccgat ccccggattc atgctattgg
    40801 tcggatcatt ctcgcgatag aacttctgca acgtctccgg tgctttatac aaatgaccga
    40861 cgttatgctc ggcagggtac tgcgcgccgc gctgctgtag cagttccagc atctgttctt
    40921 ttaacgcatg cacatccacg ccttttttca ctatgtaatc ctgatggaag acatagcaca
    40981 taaaatggcc gtaatagagc ttgtgcacca gctggctgtc gatctccggc ggtaaatgct
    41041 cataccactc ggtgtcgtta cgccgcagag cgatatccag tgccagaatg tcttcgactt
    41101 catcggaatg caccgcctga taacgaattg ctgcgcccgc agcggcgaaa cggtgtaaaa
    41161 acgctttgct accttcctcc ggcgtacaga caaagaaatc gccctcggcc tgtttgaaat
    41221 aatccaccag ccacgatttg gcttcaccga cgccatcgcc tgccattttt aacagcagat
    41281 gatgctcgta tttatcgcgc cagtttttca tgcgcggcgg taaatggctg gggaacaggt
    41341 gaccgaattt ttgcattgca cggtcagtaa aatgcggacg gaagaatttc actttctcca
    41401 gcatcgcatc ggtgcgtccc ttgagattaa agaagaacgg catcttgtcg gtgcctaact
    41461 tatcaatcat caggaaggtg tctttgccgt atttttccgc aatatcgtag atatcccggt
    41521 gcatatattc cccggcaacc ggcagatttt cgaagttagc cagaatatga cggcggattt
    41581 cggtcagcac ttccggctgg ttggtgccga tataaaacac ctgctgattt ttttccgctt
    41641 cgaaggtatc aagacgtacc gcaaagacgg ccagcttccc ggcgcaacca gaagattcaa
    41701 ataaccgatc cggatcggcg ttataacgtg cgggcgtgtc ggcttcaata tcacgaacgc
    41761 ggtggacata gtcataatcg tgggcgtgac gaccatcgtg acgcacatcg tcatctttga
    41821 tgcgatcgtc atccagcttg ctaaggatct gctccggcgt ttcgcccaga tcaatcccca
    41881 gatggttcac cagcgtcagt ttgccatctt catctatacg cgcgaataac gacatttcgg
    41941 tatacgccgg gccacgttgc accagcgagc cgccggagtt gttacaaata ccgccgatga
    42001 ccgatgcgcc tatacacgat gatccaatca ccgaatgcgg ttcgcgtccc agcggtttga
    42061 gggctttttc cagcgaatag agcgtggtgc ccggataggc cagcacctgt tcgcccttgc
    42121 cgagaacgtg cagcttgtcg agacgcaggg tgctgatgat cacgatatcg cgatcatagt
    42181 cgttaccgtt tggcgtcgat ccttcggtca ggcctgtatt ggcagcctgc atcagaataa
    42241 ttttgtcggc ggtgacgcag gctttcagca cccgccacaa ttccagtagt gagccaggga
    42301 aaacgacagc cagcgcgtcg ccctgaccag aacggaagcc cttgcgatag cgggccgttt
    42361 ttgcgggatc ggtgagcagg tgtgaatgac cgaccagacg ggcaagttca ttcaaaaagg
    42421 ctttattatc agttgttgtc atggaagaca ttttccactc cttgtggtgg cgaaaaaata
    42481 tcacaaacta gaatagcgcc ttgtatgaca attcagcgag tgaatatttt gaaaattcag
    42541 acaataaact gagcattctt tttctctctc catcatgctt atggcaaact acggtttttg
    42601 caccattttc gctgccacgt cgggcaacaa aggaagaaaa atccatatga aatggctatg
    42661 ttcagtagga atcgcggtga gtctggtcct gcagccagca ctggcggatg atttattcgg
    42721 taaccatcca ttaacgcctg aagcgcggga tgcgttcgtc accgatctgc tcaagaaaat
    42781 gacggttgat gagaaaattg gtcagctgcg cttaatcagc gtaggcccgg ataatccgaa
    42841 agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca acaccgtaac
    42901 ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc
    42961 tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg
    43021 tctggcctcg tcttttaacc tcgatgcggt gaaaacggtc gggcgtgtct ctgcttatga
    43081 agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc
    43141 gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct caataatggg
    43201 taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgttact cggtgatgac
    43261 cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt acaacaccgt
    43321 cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag cggggctgga
    43381 cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc cagccacctc
    43441 cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt
    43501 ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag acccggaaga
    43561 tgcggtgcgc gtggcgctga aatccggaat caacatgagt atgagcgacg agtactactc
    43621 gaagtatctg cctgggttga ttaaatccgg caaagtgacc atggcagagc tggacgatgc
    43681 tgcccgccat gtactgaacg ttaaatatga tatggggttg tttaacgacc catacagcca
    43741 tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa
    43801 agaagcgcgt gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt
    43861 accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga
    43921 cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg
    43981 gattaaaaat tccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac
    44041 cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc
    44101 gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt
    44161 ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc ggaccgatat
    44221 cactattccg caaggccaac gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct
    44281 ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc agcaggctga
    44341 tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt
    44401 gtttggcgat tacaacccgt ccggcaagct gccaatttcc ttcccgcgtt ctgtcgggca
    44461 gatcccggtg tactacagcc atctgaacac cggtcgtccg tataatgccg acaagccgaa
    44521 caaatatact tcgcgttatt ttgatgaagc taacggagcg ctttatccgt tcggctatgg
    44581 tctgagctat accactttca ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg
    44641 tgacggcaaa gtgacggcca gcgtgcaggt gacgaacacc ggtaagcgcg agggggccac
    44701 ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg tgaaacagct
    44761 gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca gcttcccgat
    44821 cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg agcctggcaa
    44881 gttcaatgta tttatcggca ctgattccgc acgcgttaag aaaggcgagt ttgagttgct
    44941 gtaatgcttt agtaagggcg cgacgtttgc gccctttgcc tgatgttcat atcttaactt
    45001 gctgaatttt cgccctttgt aggccggata aggcgctcgc gccgcatccg gcaggaaacg
    45061 ccggaccgaa tatgcctctt aagtatttat ctgattctgc aactgaatcc ttccgctcag
    45121 gctaactctg tcattatcaa ctatgctttt ctcttaattc gctgaaaaca acagtaaaag
    45181 aatgaggaaa gcatcatgcc actcttaaag ctctgggcag gttcactggt tatgttggca
    45241 gccgtgagcc tgccgctaca ggcggcttcc cccgttaaag tcggttcaaa aatcgatacc
    45301 gaaggcgcgc tgctcggcaa catcatcttg caggtactcg aaagccacgg cgtaccgacg
    45361 gtgaataaag tgcaactcgg aacgactcct gtggggcgcg gggcgattac ttccgatgaa
    45421 ctggatatct atccggaata taccggcaat ggcgctttct tctttaaaga tgaaaacgat
    45481 gcagcgtgga aaaatgccgg gcagggctac gagaaagtca aaaagctcga tgcggagcac
    45541 aacaagttaa tctggctgac gcccgcgcct gcaaataaca cctggactat cgccgtgcgt
    45601 caggatgtgg cagagaaaaa caaactcact tcgcttgccg acctgagtcg ttatctgcaa
    45661 gagggcggca ccttcaaact ggcagcctcg gcagagttta tcgaacgcgc cgatgcgtta
    45721 cccacgtttg aaaaagccta tggctttaag ctcggtcagg atcagttgct gtcactggct
    45781 ggtggcgaca cggcggtgac gattaaagcc gctgcccagc aaacctctgg cgttaatgcc
    45841 gcaatggctt acggcactga cggtccggtc gcggcgctgg ggctgcaaac cttaagcgat
    45901 ccgcaaggcg tgcaacctat ctacgcgcct gcaccagtgg tgcgtgaatc ggtgttgaag
    45961 gagtatccgc aaatggcaca gtggctacag ccagtcttcg ccagcctcga tgcaaaaaca
    46021 ttgcagcaac tgaatgccag cattgcagtg gaaggactgg atgccaaaaa agtggctgcc
    46081 gactacctga aacaaaaagg gtgggcgaag taatttcccg tgacttatct acgtattaat
    46141 cctgttctgg cgctgctgtt gttgctgacg gcaatcgcag cggcgctgcc gtttatcagt
    46201 tacgcgccta atcgtttagt ctcgggtgaa gggcttcatc tctggcaact gtggccgcaa
    46261 acgatctgga tgctggtggg cgttggttgc gcctggctga cagcctgttt tgtccccgct
    46321 aaaaaaggca gcatttttgc actcattctg gcgcaattcg tcttcgtatt gttggtgtgg
    46381 ggagctggaa aggcggcgac ccaactggcg caaaatggca gtgcgctggc gcgtaccagc
    46441 ctcggcagag gtttctggct ggctgcggcg ctggcattgc tggcctgtag cgatgccatc
    46501 cgccgaatct ccacgcatcc gctgtggcgc tggttgttgc atatgcagat tgccattatt
    46561 ccgctgtggt tgctgtactc cggcacgctt aacgatctct cactaatgaa agaatacgcc
    46621 aaccgtcagg atgtgtttga cgacgcgctg gcacaacatc tgacgttgct gtttggtgcg
    46681 gtgctgcctg cgttagtgat tggtgtgccg ttgggcatct ggtgctactt ttccactgcg
    46741 cggcaggggg caattttttc tctgctcaat gtcattcaga ccgtgccttc ggtggcgctc
    46801 tttggcctgt tgattgcgcc gcttgccgcg ctggttacgg cctttccgtg gctggggaag
    46861 ctcggcatag caggaaccgg aatgacaccc gcactgattg cgctggtgct ctatgccttg
    46921 ctgccgctgg tgcgaggcgt ggtagtcggt ttgaaccaga tcccgcgcga tgtgctggag
    46981 agcgccagag cgatggggat gagcggggcg cagcgtttcc tgcatgttca gttaccgctg
    47041 gcgttaccgg tatttctgcg cagcctgcgg gtggtgatgg tgcaaactgt aggtatggcg
    47101 gtgattgcgg cgttaatcgg cgcaggcggt tttggtgcgc tggttttcca ggggctgcta
    47161 agcagcgcca ttgatttagt gttgctgggg gtgatcccgg taattgttct ggcggtgctt
    47221 accgacgcgc tgttcgattt gcttatcgca ctgctgaagg tgaaacgtaa tgattgaatt
    47281 tagccatgtc agcaaactgt tcggcgcaca aaaagccgtt aacgatctca atctcaattt
    47341 tcaggaaggg agtttttcgg tgctgattgg cacatctggc tccggcaaat ccaccaccct
    47401 gaaaatgatt aaccgcctgg tggagcatga cagcggagtg atccgctttg ccggagaaga
    47461 aattcgctcg ctgccagtac tggagttgcg ccgccggatg ggctatgcca ttcaatctat
    47521 tggcctgttc ccccactgga gcgtggcgca aaacatcgct accgtgccgc aattacaaaa
    47581 atggtcgcgg gcgcggattg acgatcgtat cgacgaatta atggcgctac tggggctgga
    47641 gtcaaatttg cgtgagcgtt atccgcatca gctttccggt ggtcagcagc aacgtgtggg
    47701 agtggcgcgt gcactggctg ccgatccgca agtcttactg atggatgaac cttttggcgc
    47761 actggacccg gtaacgcgcg gcgcgttgca acaagagatg acgcgcattc accgtttgct
    47821 ggggcgcact attgtgctgg tcactcatga tattgatgag gcgctacggc tggcagaaca
    47881 tctggtattg atggatcacg gtgaagtggt gcagcagggg aatccgctga cgatgctgac
    47941 tcgtccggcg aatgattttg tccgccagtt ttttggacgt agtgaactgg gggtgcacct
    48001 gctttcgtta cgtagcgtgg cggattacgt gcgtcgcgaa gaacgggcag aaggtgaggc
    48061 actggcagaa gagatgacgc tacgcgatgc gctctccctg tttgtcgcgc ggggatgcga
    48121 ggtgctgccg gtggtgaaca cgcagggcca gccttgcggc acgctgcatt ttcaggatct
    48181 gctggtggag gcgtaagcgt atgaagatgt tgcgcgatcc gctgttctgg ctcattgctc
    48241 tttttgtggc gctgattttc tggctgcctt acagccagcc gctgtttgct gccttgttcc
    48301 cacaactgcc acgacccgtt tatcagcaag aaagttttgc agctctggca ctggctcatt
    48361 tctggctggt gggaatttcg agtttgtttg cggtgatcat tggcacaggt gccggaattg
    48421 ctgttactcg cccgtggggc gcggaatttc gcccactggt ggaaactatt gccgccgttg
    48481 gacagacttt tccgcctgtc gcagtgctgg cgattgccgt tccggtgatc ggctttggtc
    48541 tgcaaccagc gattatcgcc ttgatccttt acggtgtgct gcccatcctg caggtgacac
    48601 ttgccgggct gggagcgatt gatcccagcg tgacagaagt tgcgaaaggt atgggaatga
    48661 gtcgaggtca gcgactgcgt aaggtcgagc taccgctggc ggctccggtg attctggcgg
    48721 gcgtgcgaac ttcggtgatt atcaacattg gtacggcgac gatcgcctca acggtagggg
    48781 ccagtacgct gggtacgccc atcatcatcg ggcttagcgg atttaatacc gcgtatgtga
    48841 tccagggggc gttactggtg gcactggcgg cgatcatcgc agaccgcctg tttgaaaggc
    48901 tggtgcaggc gcttagccag cacgcaaaat aaaggtataa cctgcgagca tgacgccacc
    48961 aattccgcct aacgccataa acaggaacag ggcgatgacc ccaattttag ctatgcgcat
    49021 aatgcactcc ttatgttaac gaaaggattg tacagtaaag cgcatttgtt aacgaatcat
    49081 taaataccga gtgggaaaat atcatggcct tgttcttgcc aactggtgag ttgctgctgt
    49141 tgggcggagg ttcgattttc accgcaccac accagcaatg tacggccttc gaatagttca
    49201 gggcgtagtt gattgagcga gtgggcgagc gaggacatca atgcgccatc cttgttgact
    49261 ggcaatccag ccctccagcc acagacgggt ggtatcctga atattccagc caaccaccag
    49321 cgcatcttta ccctgttttt tacgtgccga agccagacaa atggcgatgt agttgatcag
    49381 tacgccgtcg aggatcgcca gcagcgcctg gagagtcggt tgttggcact gaagccgtcg
    49441 gcgcagagga ataaacagat gtgtggtgag tgtctgggcg gggtaatcct gaccgcgctc
    49501 tttgatccac gttcgcaggc tatgtagatt gcc
//
LOCUS       DAJTRW010000021        48277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRW010000021 DAJTRW010000000
VERSION     DAJTRW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            132..425
                     /locus_tag="OO326_001308"
     CDS             132..425
                     /locus_tag="OO326_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5965750.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(466..1656)
                     /gene="nepI"
                     /locus_tag="OO326_001309"
     CDS             complement(466..1656)
                     /gene="nepI"
                     /locus_tag="OO326_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5965751.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1867..2319)
                     /locus_tag="OO326_001310"
     CDS             complement(1867..2319)
                     /locus_tag="OO326_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5965752.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2372..3706)
                     /gene="adeQ"
                     /locus_tag="OO326_001311"
                     /gene_synonym="yicO"
     CDS             complement(2372..3706)
                     /gene="adeQ"
                     /locus_tag="OO326_001311"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5965753.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3881..5647
                     /gene="adeD"
                     /locus_tag="OO326_001312"
     CDS             3881..5647
                     /gene="adeD"
                     /locus_tag="OO326_001312"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5965754.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5693..7084)
                     /gene="uhpT"
                     /locus_tag="OO326_001313"
     CDS             complement(5693..7084)
                     /gene="uhpT"
                     /locus_tag="OO326_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5965755.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7222..8541)
                     /gene="uhpC"
                     /locus_tag="OO326_001314"
     CDS             complement(7222..8541)
                     /gene="uhpC"
                     /locus_tag="OO326_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5965756.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8551..10053)
                     /gene="uhpB"
                     /locus_tag="OO326_001315"
     CDS             complement(8551..10053)
                     /gene="uhpB"
                     /locus_tag="OO326_001315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5965757.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10053..10643)
                     /gene="uhpA"
                     /locus_tag="OO326_001316"
     CDS             complement(10053..10643)
                     /gene="uhpA"
                     /locus_tag="OO326_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5965758.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10718..11008)
                     /gene="ilvN"
                     /locus_tag="OO326_001317"
     CDS             complement(10718..11008)
                     /gene="ilvN"
                     /locus_tag="OO326_001317"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5965759.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11012..12700)
                     /gene="ilvB"
                     /locus_tag="OO326_001318"
     CDS             complement(11012..12700)
                     /gene="ilvB"
                     /locus_tag="OO326_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5965760.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12806..12904)
                     /gene="ivbL"
                     /locus_tag="OO326_001319"
     CDS             complement(12806..12904)
                     /gene="ivbL"
                     /locus_tag="OO326_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5965761.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13469..13558
                     /gene="tisB"
                     /locus_tag="OO326_001320"
     CDS             13469..13558
                     /gene="tisB"
                     /locus_tag="OO326_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5965762.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13838..15022
                     /gene="emrD"
                     /locus_tag="OO326_001321"
     CDS             13838..15022
                     /gene="emrD"
                     /locus_tag="OO326_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5965763.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15030..15527)
                     /locus_tag="OO326_001322"
     CDS             complement(15030..15527)
                     /locus_tag="OO326_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5965764.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15524..15886)
                     /locus_tag="OO326_001323"
     CDS             complement(15524..15886)
                     /locus_tag="OO326_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5965765.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15876..16223)
                     /locus_tag="OO326_001324"
     CDS             complement(15876..16223)
                     /locus_tag="OO326_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5965766.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16332..16745
                     /locus_tag="OO326_001325"
     CDS             16332..16745
                     /locus_tag="OO326_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965767.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16792..18234)
                     /locus_tag="OO326_001326"
                     /pseudo
     CDS             complement(16792..18234)
                     /locus_tag="OO326_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18269..19984)
                     /locus_tag="OO326_001327"
     CDS             complement(18269..19984)
                     /locus_tag="OO326_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5965768.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGVEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20151..21044
                     /locus_tag="OO326_001328"
     CDS             20151..21044
                     /locus_tag="OO326_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5965769.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21041..21855)
                     /locus_tag="OO326_001329"
                     /pseudo
     CDS             complement(21041..21855)
                     /locus_tag="OO326_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21855..23471)
                     /locus_tag="OO326_001330"
     CDS             complement(21855..23471)
                     /locus_tag="OO326_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5965770.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23766..24440
                     /locus_tag="OO326_001331"
     CDS             23766..24440
                     /locus_tag="OO326_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5965771.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24479..26140)
                     /locus_tag="OO326_001332"
     CDS             complement(24479..26140)
                     /locus_tag="OO326_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5965772.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26336..26764)
                     /gene="ibpB"
                     /locus_tag="OO326_001333"
     CDS             complement(26336..26764)
                     /gene="ibpB"
                     /locus_tag="OO326_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5965773.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26876..27289)
                     /gene="ibpA"
                     /locus_tag="OO326_001334"
     CDS             complement(26876..27289)
                     /gene="ibpA"
                     /locus_tag="OO326_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5965774.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27283..27355)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27594..27926
                     /locus_tag="OO326_001335"
     CDS             27594..27926
                     /locus_tag="OO326_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5965775.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27928..29160)
                     /locus_tag="OO326_001336"
     CDS             complement(27928..29160)
                     /locus_tag="OO326_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5965776.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29261..30361
                     /gene="cbrA"
                     /locus_tag="OO326_001337"
     CDS             29261..30361
                     /gene="cbrA"
                     /locus_tag="OO326_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5965777.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30322..31659)
                     /locus_tag="OO326_001338"
     CDS             complement(30322..31659)
                     /locus_tag="OO326_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5965778.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31734..33503)
                     /gene="dgoD"
                     /locus_tag="OO326_001339"
                     /pseudo
     CDS             complement(31734..33503)
                     /gene="dgoD"
                     /locus_tag="OO326_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701293.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(33487..34365)
                     /gene="dgoK"
                     /locus_tag="OO326_001340"
     CDS             complement(33487..34365)
                     /gene="dgoK"
                     /locus_tag="OO326_001340"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5965779.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34362..35051)
                     /gene="dgoR"
                     /locus_tag="OO326_001341"
     CDS             complement(34362..35051)
                     /gene="dgoR"
                     /locus_tag="OO326_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5965780.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35329..35985
                     /locus_tag="OO326_001342"
     CDS             35329..35985
                     /locus_tag="OO326_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965781.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36031..36843)
                     /gene="yidA"
                     /locus_tag="OO326_001343"
     CDS             complement(36031..36843)
                     /gene="yidA"
                     /locus_tag="OO326_001343"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5965782.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36957..37355)
                     /locus_tag="OO326_001344"
     CDS             complement(36957..37355)
                     /locus_tag="OO326_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5965783.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37595..40009)
                     /gene="gyrB"
                     /locus_tag="OO326_001345"
     CDS             complement(37595..40009)
                     /gene="gyrB"
                     /locus_tag="OO326_001345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5965784.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40038..41111)
                     /gene="recF"
                     /locus_tag="OO326_001346"
     CDS             complement(40038..41111)
                     /gene="recF"
                     /locus_tag="OO326_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5965785.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41111..42211)
                     /gene="dnaN"
                     /locus_tag="OO326_001347"
     CDS             complement(41111..42211)
                     /gene="dnaN"
                     /locus_tag="OO326_001347"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5965786.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42216..43619)
                     /gene="dnaA"
                     /locus_tag="OO326_001348"
     CDS             complement(42216..43619)
                     /gene="dnaA"
                     /locus_tag="OO326_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5965787.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43916..43996
                     /locus_tag="OO326_001349"
     CDS             43916..43996
                     /locus_tag="OO326_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965788.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44226..44366
                     /gene="rpmH"
                     /locus_tag="OO326_001350"
     CDS             44226..44366
                     /gene="rpmH"
                     /locus_tag="OO326_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5965789.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44383..44742
                     /gene="rnpA"
                     /locus_tag="OO326_001351"
     CDS             44383..44742
                     /gene="rnpA"
                     /locus_tag="OO326_001351"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5965790.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44706..44963
                     /gene="yidD"
                     /locus_tag="OO326_001352"
     CDS             44706..44963
                     /gene="yidD"
                     /locus_tag="OO326_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5965791.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44966..46612
                     /gene="yidC"
                     /locus_tag="OO326_001353"
     CDS             44966..46612
                     /gene="yidC"
                     /locus_tag="OO326_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5965792.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46718..48082
                     /gene="mnmE"
                     /locus_tag="OO326_001354"
     CDS             46718..48082
                     /gene="mnmE"
                     /locus_tag="OO326_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5965793.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11960 a        13061 c        12206 g        11050 t
ORIGIN      
        1 aataaaagtg gaatataaag tctggcactt cttactgacc acgcaagcgc gtttcgtaca
       61 gcatgatgag agcgatgaaa gcaaactaca cttatgcttc attcgataca catttgtaaa
      121 aggttgaatc catgaaacca acgacactac tgcttatttt cacttttttc gctatgccag
      181 gcatcgtata cgccgagtcg ccttttagct cactgcagtc cgcaaaagag aaaaccacgg
      241 tattacaaga tttacgtaaa atttgcacgc cacatgcgtc attatcagat gaagcgtggg
      301 aaaagttaat gttgtcagat gaaaacaata aacaacatat acgcgaggcc atcgtggcga
      361 tggaacgtaa taatcagagc aattattggg aagcgttggg taaggtagaa tgcccggata
      421 tgtaagaaca gcaacaacta ctgaccaacg cttctttacc tgatatcagg atttcttcat
      481 tttcaccttt gcagtaacca acaatgcagt cagcaacatc aatgtgccgg acaacatcaa
      541 cggcgaagtc agaccaatat tatccagcgc ataaccgccg attgccgcgc cacaggtatt
      601 agcaagctga ataaccgcca cctgaataga cccggctttt tctgcctgat cggccagcga
      661 gcgggtgatc cacgttgacc agccgacggg aaccaatgca aaagttagcc cccagataat
      721 cgccacgccg gtagcaacga ttttatcgct tccccacaac gtcagtacca acgcactcac
      781 agccagtatt aacggcgcgc ctgctaaggc cagttttacc gaacgtttaa gaatgaacga
      841 cgaaagcgac gtaccaataa agctggcgat accaaaactc aacagcacca gcgttaagcc
      901 atccacgccg aatcccgcca ggttcatata caccgggcga atatacgtga agaaagcaaa
      961 ctgcccggcg aaagacatga agatggcgat catccctgcc atcacacccg gacgttgtaa
     1021 taagcggaaa gtattttgtt tctgatgcga gggttcgcct ggcagtgaag gcaatgattt
     1081 gataatccag aaaatacaca gcacgcccat caccgccgcc gcattaaaga cattgcgcca
     1141 accgataagc tcgcctaaaa aactgcccaa cggcgcggca atcaccagcg caatagaaac
     1201 cgcgccgaag atcaccgaca gcgccttcgg caccgtacgc ggcggtacca gacgcatggt
     1261 cagcgacgcc gacatcgccc agaacccgcc cagcgccagc cccagacagg cacgaccgat
     1321 taaaagcaaa ctgaatgagt tagcaaagga aaccagcaag caggagagcg tcagcaaaac
     1381 ggcaaacaaa ataacaacgt agcggcggtc agtggcctga attgtctggg taataaacaa
     1441 actggcaaac attgccacaa aggcggtcac ggtcaccgat tgcccggcaa ccccttccga
     1501 aatgcctaaa tcctgggcca ttggcgtcaa caaactgacg ggcaaaaact caacgataat
     1561 cagacaggcg acacaaaacg ccaccgagaa aacggctgac cagttcggtc gggtgatggc
     1621 atccgcgccg cggttttcgg caataaattc actcatggtg ttacccgtgg catgtttcag
     1681 gggaaaagcc gaccacttta acattgaaat gtgtgacgaa tttaacgttt ttgcaacttt
     1741 tacggtgttg cttgagctgg agttaagatc gaaacggaac agggcaaaga ttaccggatg
     1801 cggcgtgaac gtcttatcca cccgacaggt caggcgcggt ctgtaggctg ataagacgaa
     1861 acggtttcaa tgcaaccagt gaacgccctc ggcaggctga aagaacgcgt tatagcgcat
     1921 ctgaaaagca ttaatatcct gcatttcagg ttcggtttca cgcagaaaac ccagcgccag
     1981 cctgacctga gcatcggtaa tcggcgcgga aaggcgggct ttttgtaaaa tccggtgcca
     2041 ttgcagcagg ttctgttcgc tatcatcgac aatgacaatt tgccagataa gcaacacctg
     2101 cgcggcgttt tgcaggtgcg actcatccgg gcgatgcaaa taacgtaaaa attcgtcacc
     2161 ggctgacttt cccatttgat cgaccatcga ctccacgggt acgacatcga tattcagccg
     2221 atcgctgaga cggcgaatcg cttttctggc cccggatgtc agcacccgaa aaccaaccac
     2281 aaacactacc gccagcgtgg cgagcattat ccagatcatg agttctcctg cgtaatcaga
     2341 actcatgata tctggaaacc ctcgccagtt actaatccac cagaataatc ttcaatgcga
     2401 acagagctgc gaccaccacc acacacaggt tcagatcgcg ccagcgcccg gtgcacactt
     2461 tcatgatgca gtacgacata aagccgagtg caatcccttc ggtgatcgag aaagtaaagg
     2521 gcatcatcac cgtggtaata aacgcaggca ccgattcggt aaaatcatcc cagttaacgc
     2581 gcgccaggct cgaagtcatc agcacgccaa caaagattaa cgctccagcg gttgcgtaag
     2641 gaggaaccat cgccaccagc ggtgagaaga acataactaa caggaacata acgccgacca
     2701 cgacagccgt tagccccgtg cgaccaccga ctgccacacc agaagtactt tcaatatagg
     2761 cagtaacaga cgaggtaccg ataaatgcgc ctgccaccga actgacgcta tcaacataca
     2821 gcgccttatt catattgggg aatttaccgt tgctatctat taagcccgct ttatcagtta
     2881 caccaattaa tgttcctgat gaatcaaata ggttgatcag cataaaggag aaaatgatac
     2941 cggcgagttc aagtgttaac gcgccgctca aatctacttc accaatgacg ccgctaatat
     3001 caggcggaat ggaatagacg ccgctaaaat gaacatcacc gaaaaataat ccacagcaag
     3061 acgtcaccac aatagaaacc agcaccgcgg catgaaaatg acgtgatgac aacacggtta
     3121 taataaaaaa ccctaaaata cctaacaaca cgccgtgaga acttaaatcg ccaatcatca
     3181 ccagcgtgtc tttattggcg acaataacac cagtgttttt taatcccatt aaggcaataa
     3241 ataatccgat tccactggtg ataccaatac gtaaacttaa tggaatgttg gagatcatcc
     3301 agtaccggat acgaaacagc gtgagcaaaa atagcccaat tgcgccccag aatatcgcgc
     3361 ccatcccggt ctgccaggag atccccatcg cccccacgac cacgaacgca aagaaggcgt
     3421 tcagccccat tgccggagcc agcgccacgg gtaagttagc aaatatcccc atcgcaatac
     3481 tgccgatacc ggcaatcaaa caggtggtaa caaacaccac tttcgggtcc atttgtgccg
     3541 cgccgaggat ttgcgggttc acaaaaacaa tgtacaccat ggttaaaaaa gtggtcatcc
     3601 ccgcaatcaa ttctgtgcgg acggtggtcc catgctgagg tagtttaaat aatcgcgaaa
     3661 gcgtccctga ttcattactc acgtaatcgg tattgtcatt attcattttt ttgtccattg
     3721 gaggagattt aatcccttgc ttttgtaaaa caaatgacaa caacggttca gtgataatta
     3781 tcacatttca attgcacatt aatagatatt ctttaataat ctcgcgacgt ttctttatga
     3841 taaataataa tcaaattgat aaaatcaaaa tgagaaaaat atgaataatt ccattaacca
     3901 taaatttcat cacattagcc gggctgaata ccaggaattg ttagccgttt cccgtggcga
     3961 cgctgttgcc gattatatta ttgataatgt ctctattctc gacctgatca atgccggaga
     4021 aatttccggc ccaattgtga ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc
     4081 tgatactccg gctttgcagc ggattgatgc tcgcggcgca acggcggtgc cagggtttat
     4141 tgatgctcac ctgcatattg aatccagcat gatgacgccg gtcacttttg aaaccgctac
     4201 cctgccgcgc ggcctgacga ccgttatttg cgaccctcat gaaatcgtca acgtgatggg
     4261 agaagccgga ttcgcctggt ttgcccgctg tgccgaacag gcaaggcaaa accagtactt
     4321 acaggtcagc tcttgcgtgc ccgctctgga aggctgcgat gttaacggtg ccagttttac
     4381 ccttgaacag atgctcgcct ggcgggacca tccgcaggtt accggccttg cagaaatgat
     4441 ggactaccct ggcgtaatta acgggcagaa tgcgctgctc gataaactcg atgcatttcg
     4501 ctacctgacg ctggacggtc actgtccggg tttgggtggc aaagaactta acgcctatat
     4561 tgctgcgggt attgaaaact gccacgaaag ttatcagctg gaagaaggac gccggaaatt
     4621 acaactcggc atgtcgttga tgatccgcga agggtccgct gccagcaatc tcaacgcact
     4681 ggcaccgttg atcaacgaat ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa
     4741 cccgtgggag atcgcccatg aagggcatat cgatgcctta attcgccgcc tgatcgaaca
     4801 acacaatgtg ccgctgcatg tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt
     4861 tggtctgaat cacctcggct tactggcacc cggcaagcag gccgatatcg tcctgttgag
     4921 cgatgcgcgt aaggtcacgg tgcagcaggt actggtgaaa ggcgaaccga ttgatgcgca
     4981 aaccttacag gcggaagagt cggcgagact ggcgctgtct gccccgccat atggcaatac
     5041 cattgcccgc cagccagttt ccgccagcga ctttgccctg caattcacgc ccggaaaacg
     5101 ctatcgggtc attgacgtca tccataacga attgattacg cactcccgct ccagcgtcta
     5161 cagcgaaaat ggttttgatc gcgatgatgt gtgctttatt gccgtacttg agcgttacgg
     5221 gcaacggctg gctccggcct gtggtttgct cggcggcttt ggcctgaatg aaggcgcgct
     5281 ggctgcgaca gtcagccatg acagccataa tattgtggtg atcggtcgca gtgccgaaga
     5341 gatggcgctg gcggtcaatc aggtgattca ggatggcggc gggctgtgcg tggtacgtaa
     5401 cggccaagta caaagtcatc tgccgttacc cattgccggg ctgatgagca ccgacacggc
     5461 gcagtcgctg gcggaacaaa ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt
     5521 acccgatgag ccgtttattc agatggcgtt tctttctctg ccagtgatcc ccgcgctaaa
     5581 actaaccagt caggggctat ttgatggcga gaagtttgcc ttcactacgc tggaagtcac
     5641 ggaataataa aaaaagcccg gcgtcatgcc gggcaaaagt caccagttac gtttatgcca
     5701 ctgtcaactg ctgaattttt ttctcgcggc ggattttgcg ttcttccatt accgccacta
     5761 tcgccatcag gcagatacaa ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc
     5821 agccggtaag gccgaatacc ggcgtcccat cggcaatcat tcccagacct aacttggcaa
     5881 agctgtcacc aatcaggtaa gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt
     5941 ttttaggtac aaagccaaca gcagccacac caatcaacaa ttgcgggcca aagaccagga
     6001 aacccaacgc aaagagagaa gccagataga tatattcgtt actggcatgt tgatacacac
     6061 cgagcgtggc gataatcagc gccagcgcga tgcaggccac caggccacgg cgaccgttcg
     6121 ccaggtcaga gagccagccc cacagcagcg taccgaccag cgcaccagct tcaaacagcg
     6181 taaagccctg aatcgccacc gctttagaga gtttcagttc ctggaacgcg tatacggttg
     6241 accactggtc gataccaata cgtaccacat agaggaaaat gttggcgaag cacagcagcc
     6301 agatcacttt gtttttcagc acatactcaa caaagatctg ccacttggtc atatcggtag
     6361 attctgtctc tttgtcctct tcgctgatct cctcgccgaa cagttcttca gctttgccga
     6421 ggccataaga ttccggggag tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg
     6481 cgataatcga cgggaagata aacatgccga tgacatggcc atcgaacagg taatttgccc
     6541 cgaacagcgc cacacctgct gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac
     6601 cgaggaatgt cccgcgttta cgacgcggcg tccatttggt gatggtggag taactgcacg
     6661 aaccgccggt actctggaaa aagccgctta aggcgtagaa ggcaatcatc aggaacaggc
     6721 taaccgagcc gctgcccata ctggcactga agcccagcat acaaatggca gagaggataa
     6781 gcatgaacgg caggaattgt ttggtgtttt tgccgtcggc gtagtatgaa accaacgttt
     6841 tacccacgcc ataagtgatg gagaaaccca ggccgatcat ccccagctgc gtcatgctca
     6901 acccgtaggt cgaaatcata tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca
     6961 tcgtcaggta gccgataaag accaccaggt aggattgcat gaacggtttg aaccacattt
     7021 tgcgccgcac ttcgagcgga aggtccaggg tcggcttgcg aacttggttt aggaaagcca
     7081 gcatgggtta ctcctgaaat gaatacctgc gtgaggcatg cattgtaaaa atcagcagca
     7141 aatcaggcct gagatagcgt ccaagaaaaa cctggaaaat ttcttagttt tgccggatat
     7201 gaagtgaaaa ggtgagatgc atcacgcttc acgcggtgtc tgggcgttca aaaagggcag
     7261 taacagcagt gcggaaatcc cggcggcgat agagataacc acaaaaaatc cgctccagtg
     7321 ccaggtatcg agcactttcg ccagcggcca accagcaagc gacgccccca gataagcaaa
     7381 caagccgaca aaccccgtcg ccgcccctgc cgcctctttg tgggaacact ctgccgccgc
     7441 cataccaatt aacatctgcg ggccaaagac aaaaaaacca atggtgaaga agcaggttgc
     7501 ctgcatcacg tagctggcaa atggcatcag ccacagcgag ccgactgaaa gcaaaattcc
     7561 ggcggcgaaa atcaaattca tcggcccacg gttgccgtta aacaatttgt ccgagcccca
     7621 accggctacc agcgcaccga taaatccgcc cagttcaaac atcgtcactg ccgtattcgc
     7681 cgtgaccaga tcgacgccca gcgtctcgga catatacaaa ttgccccagt cgttgatcgc
     7741 cgcccggacc acatagacca gcacatagca aaacgaaagc agccagatat acggattcag
     7801 caacacatat ttggtgagga tctctttacg cgtcaaccct gccccttctt gttgttgagc
     7861 aatttccagc gcgtcgtgcc gccattcacc gaccgccggt aaacctaacg cctgcgggcg
     7921 atcgcgtagc cgccagcaga gaaaaatccc cacgactatc gccatacaac cagcaatcat
     7981 catcccggca cgccagccgt aatgcagcgc gctcgctgcc atcacaatgg gaatgagtgc
     8041 gccgccgacg ttatgcgccg tgttccataa tgcccaccaa ccgccgcgct cggtacgtga
     8101 ataccaggcc gttaacagac gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt
     8161 cagcacccag agcacggcaa acgcccatag cgacgtcgag aagccaaaca gaatgttcat
     8221 gatgcccgtg gcgataagcc ctatccccat aaaataacgg gcatttgagc gatcgctgac
     8281 aatgccggag acaaacttcg acacgccgta agtaatgtaa aacagggtcg ctaacaggcc
     8341 gatatcgcta cggctgagca cgccgttagc aaggatttct ggtaccgcgg cgttaaaact
     8401 tttccgcgtg aagtaaaaca gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg
     8461 ccagtagcga taacgggcat caatttcatg tttatcagtc attaatggcg catcggcagg
     8521 cgctttcaga aacggcaaca tcatcgaacc ttagacataa cgttgaggta gagaaacgct
     8581 gacacgcgtg ccgtgcagac aggaaatgtg taatgtgccg cccagcgccg ttacgcgctc
     8641 gcgcattccg gtgaggccaa aaccttgttg cccggaaccc ggcggcaaac cgctgccatc
     8701 gtcttcaatc accagcatca accgttcatc ctgctgccag ccttgcagcg taaccgcgct
     8761 ggcatcggca tgtttcacaa tgttgttcag cccttcctgg cagacacgaa acagcgtcac
     8821 gcgctggttt tcgcttaatg ctgattcatc gatacgccat tcgagatggc tgacaatgcc
     8881 gcgcccttcc agctccattt cccgcatcag tgagcggatg gcctgctcca gggtgagatc
     8941 atccagctgg cgcggacgta accgtcctaa caaacggcgc accgcgtcgt aaacgcccag
     9001 cgacagttgt tcgatgagct gcccgctctg cttcacactg gcgttatctg ccgccagccg
     9061 ctgaacaatg cccgcctgag tacgaatagc agtgatggtc tgaccgatat cgtcgtgcag
     9121 ctcacgcgcc acgtcgcggc gcacgctctc ttcggtttct aacaaacgtt cagccagatg
     9181 ctgattgcgc gccagttcct tttgtagcga ctggttaagt tcacgcaacc gctggatgcc
     9241 tgcgcccagt aacaaccctg tcagactttg caccagcaac gagagtaata aatccaccag
     9301 atgatcgcgc caggtttgac tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag
     9361 cgctccttgc cagccatagt gccaggccag cgcgataatc ggcagcgcca gacagaatgg
     9421 cgtaaagcgc gacagttcgt ccggcaatcc caactggagc cagagactga taacaaacag
     9481 cagcaagtac cagaccagat gccgcccgcg ccagttgatg ggctgagaaa ccagtgacgg
     9541 gccgagcggc agccaggtgt tattggcgag atagtgccag aacaccagac atatcggggc
     9601 cagcgtcaga ccgccagtta aagtcagcaa cagcgcattc cacgactctt tgccgtgcca
     9661 aagccagggc agcgactgca acaacgccgc cgccgttaac gccgcaccct gtaacagcaa
     9721 ggtgcgccag tcacgctgat ggcgatagcg cgagatcagc gctacgggca gtaacgtcag
     9781 caaactaccg atcatcaata acggaaaatg ggttaaaccg accgcctgcg ttagccagta
     9841 aatcagcagc cactccgcac ccagcaatac gggccagtat ccgcgcgggc attgcagcat
     9901 tagccccaga cgcagaccaa acggaaataa cagcaccgcc atatcagggc gctcaaccag
     9961 atgcaggctg atactccata ggcaaaacca tgcggcagag aagataaaaa agcaggcaat
    10021 aacggtaatt aagcgggaga acaacgtctt catcaccagc catcaaacat gcggcgcgcc
    10081 agctctacgt cgttactgac gcccagtttt tccatcagat tggcgcgatg gacgtgtacc
    10141 gttttcggtg acaagcccag ttcggcggca atctctttca ccgccattcc ttgcgccagt
    10201 ttttccgcca cctggcgttc acgtttggtc agcgggtcct gacgaccgga tgccagttta
    10261 atggcaatat ccggcgtcag ataacagccg cccgtggcaa ccgtatgcac cgcagcaatg
    10321 agttcatccg ggctacagcg tttggaaaga aagccgcgtg cccccgcgtt aagcgcctgc
    10381 tcaaccagcg caggactgtc gtgaacggag agcataatcg tcgccatacc tttcggcagc
    10441 tggcttagca gctccagacc ggagatatcg ggcatggaga tatcgcaaat acacacctgc
    10501 acaccgcgcc ccggcagccc cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc
    10561 tgcaaatcag gttccagccc cagcagctgc gcaaagccgg agcggacgat gaggtgatcg
    10621 tctataaggg caacggtgat catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc
    10681 ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac accgcgatct
    10741 tgttaaacat cgtcggatcg gactgattac gctgcacttt cacgacatct tccagcttag
    10801 cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc cagatatggc
    10861 ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg
    10921 caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg agctccagaa
    10981 ttacgttgtt atgagttgtg ttttgcatgg cttattcccc caccatttca gtattcgccg
    11041 cacctggcgg caccatcggg taaacttttt cttcggcatc aatgcgcaca tggatcagcg
    11101 ccgggccagg gcgattgatg atttcctgca atgcagcctg cggatcggct tcgttattca
    11161 aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt ttgcccggat
    11221 aggtggcggc aaaaacgcct tgctcgtaga acagactctg ttgctgatgc accagcccca
    11281 gcgcttcgtt gttcatcaga atgattttga catccagctg attttcactg gcggtcgcca
    11341 tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact ttgcgatccg
    11401 ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg cccagcccac
    11461 cggaggtcag ccactggcgt gggcgattga gcggataagc ttgcgcggtc cacatctgat
    11521 gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca acggcgttga
    11581 tcaggccgta atggcttaat ggatcgcacg ctttcgggat tggacacggg aattcgcgct
    11641 gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc cgcgggatca
    11701 attgcgccag cacgtcatca acatccgcct gaatcgccac atgcggctgc ttgattttac
    11761 ccagctctgc gcgatcgata tcgacatgaa taattttggc attcggacag aactgctcgg
    11821 ttttgccaat cgcccggtca tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct
    11881 gcaaaatata gttggtactg cgcacgccgt gcatccccag catacccagc gacaacggat
    11941 gcgcttttgg cagcatgccc agcgccatta aagtcatggt ggtaggcagt tgcgctttct
    12001 ccgccagttc acgcacccgc gcaggcgcat tgatcacacc accacccaga taaagcaccg
    12061 ggcgtttggc agcgttaatc attgtagctg cgtcacgaat gctttcttcg ctaaaggcgg
    12121 gtgcaacggc tttttctgcc accgcgggct gagcttcaat ctcaaaaacc gccgtttgta
    12181 catccttagg aatgtctatc cacaccgggc tagggcggcc tgattgcgca atgcggaagg
    12241 catcgctcat gacctgtggg agctcttcga tatgtctgac cagatagttg tgtttggtga
    12301 tggggataga gatgccgtag gtgtccactt cttggaaggc gtcggtgccg atcatcgagg
    12361 cgggaacctg accagtgatg caaatcagcg ggatggagtc cagccgcgca tcagcaatgg
    12421 cggtcaccag gttagtcgca cccggcccgc tacaggccat acagaccgcc ggtttaccgt
    12481 cggtgcgcgc cattccctga gcgataaacc ccgcgccctg ttcatggcga gccagaatat
    12541 ggcggatttg cgtgctttgg cttaaggcgt cgtaaacagg caggatagaa ccgcccggaa
    12601 tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg
    12661 taaagcgctt acgcgtcgat gttgtgcccg aacttgccat gctccagtcc ttttcttctg
    12721 ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt
    12781 ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc accacacgca
    12841 cgacgaccac tgcggcggat ggcgcagttg gtagtagttt tgcgttgaac atggaagtag
    12901 tcattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca gctttacagg
    12961 ggagacaatg gaaaattttt cagcaaggga aaattgaggg gttgatcacg ttttgtactg
    13021 aattgcagat aacaaaaaac cccgccggag cgaggtttcg tcagccgcct gcggctggta
    13081 accgcaaagc acactgtatt atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg
    13141 caatttcggc acgaattttg acgtatttag tgcatagttg agtatcgttc acagtttgcg
    13201 ttttgtccaa atattactgt ttatttatac agtaagcttc tataatatca ctgtacgcaa
    13261 tgtgttatgc gggggccgca tcgttacccg gcgcactaag tcctggctga aacgggtggt
    13321 gccgtcagcg ccttaacccc gcgtgagcac actgtgttat gtcaacaagc acaacgtttc
    13381 tccttgagat accgcgtgca caacagctgg caacaggcag cggaaaggta cgtcagctgg
    13441 cagtgctcct gaaccacagg agacgcgtat gagcctggtg gatatcgcca ttcttatcct
    13501 caaactcatt gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt
    13561 cagattcaag tcgcaccaaa ggggagcggg taaccgctcc ccttttatat ttagcgtgcg
    13621 ggttggagtc ggatgcgatg ctgacgcatc ttatccgccc taccatccct cccggcaaca
    13681 tttattgccg cttttgttta catattctgc cgctaaacaa ttccccattc ctggcgtata
    13741 tctggctaac attcatcaat gtgatagatt cctctcccgc atttatggga atgcgtagtg
    13801 acttattcta attattttta taaaagcatc cgtgataatg aaaaggcaaa gaaacgtcaa
    13861 tttgttattg atgttggtat tactcgtggc cgtcggtcag atggcgcaaa ccatttatat
    13921 tccagctatt gccgatatgg cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt
    13981 aatgggcgct tatctgctga cttacggtgt ctcacagctg ttttatggcc cgatatccga
    14041 ccgtgtcggt cgccgaccag tgatcctcgt cggaatgtcc atttttatgc tggcaacgct
    14101 ggtcgcggtc acgacctcca gtttgacggt attgattgcc gccagcgcga tgcaggggat
    14161 gggcaccggc gttggcggcg taatggcgcg tactttgccg cgtgatttat atgaacggac
    14221 acagttgcgc catgctaaca gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc
    14281 accgctaatc ggcggtctgc tggatacgat gtggaactgg cgcgcctgtt atctcttttt
    14341 gttggttctt tgtgctggtg tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc
    14401 ggtcgatgca ccgcgcacgc gcctgcttac cagttataaa acgcttttcg gtaacagcgg
    14461 ttttaactgt tatttgctga tgctgattgg cggtctggcc gggattgctg cctttgaagc
    14521 ctgctccggc gtgctgatgg gcgcggtgtt agggctgagc agtatgacgg tcagtatttt
    14581 gtttattctg ccgattccgg cggcattttt tggggcatgg tttgccgggc gtcctaataa
    14641 acgcttctca acgttgatgt ggcagtcggt tatctgctgc ctgctggctg gcttgctgat
    14701 gtggatcccc gactggtttg gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct
    14761 gttctttttc ggtgccggga tgctgtttcc gctggcgacc agcggcgcga tggagccgtt
    14821 ccccttcctg gcgggcacgg ctggtgcgct ggtcggcggt ctgcaaaaca ttggttccgg
    14881 cgtgctggcg tcgctctctg cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt
    14941 gatgacctta atgggattgt tgatcgtgct gtgctggctg ccgctggcga cgcggatgtc
    15001 gcatcagggg cagcccgttt aagcgcacgt caccgcagca tcgtcatcag ctccatggga
    15061 gaacgatgct gctttatcag atcacgcatc acccgcatat gcggtgcgga gtaagaataa
    15121 aacgcctgat agcccgcaca aagcacgctg tctggcgtct cctgccgagc gtgcgggcac
    15181 aggcgatgcc agtcgcacgc ctggcagtca cggcgcggtt gcgggttttc gctgccacac
    15241 catttttgta aggtggcgtc aaaaagctca atcgagatac gactgatatc tgcccgcacc
    15301 caacactcaa acagggcaat taaaaatttc ccccacagct ctgctgtcac cgaagcggcc
    15361 gtcagatcgc cccggtcatc aaattcaaga tagggaatga gctgtacgga gcggatgccc
    15421 cgctgtttga aattgtgata aaaatcggca ggttgcagag catccttgta ctctacaacc
    15481 agcttatgcc tgtcctcttc agcatcaatg acctgacttc ctgtcatgcg actctctcaa
    15541 taaatacgat aagttgatgt atatgcgtta cagcaaacag tattgcgaga gataacaccg
    15601 cgagggagat caaaaatttg tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga
    15661 catccattaa attgcgatta cgggtgtagt gccagaggat cagcgccacg atggcgagga
    15721 tgccaatcga aatccagaat aacatgcccg cctggtgcca gttgtgtttg atagccaacg
    15781 ccatcaacgc gccgtagccc agcatggtac gaaaccacgc cagtgatgtg cgctccggct
    15841 gcagccctgg gtcggcaatg cgtctggctt tacggctatc cggcatacaa caccagtccc
    15901 ataacgatca ccgccacaac catcaatatt aagctgatga ttaataagct gttggtgtag
    15961 ggcaggtcct ctttcagacg catcgctttt tcattgcgta accagcgcag gtagccatac
    16021 attgctaacc cgccggaaaa cagacacaac aacaacgcca gcagttcacg aatgacgggc
    16081 gtggcgaaat ctggcgcaag ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg
    16141 gtacggatcc acgccagaaa agtacgctca ttagccagcg agaagcggta atcgggcgct
    16201 tctccaaggc gggaaatttt cattgttgct cctttgcaat ttcactaacg ctctatcttt
    16261 agcggttttt tttaagaatt tcgtccgccc gtttcctgat atgcttttgc catccgctac
    16321 aaggaggtca gatgggtatc atagcgcaaa ataaaatcag ttcattgggc atgctgttcg
    16381 gtgccatcgc tttaatgatg gggattatcc acttttcatt cggccctttt tccgcaccac
    16441 cgccaacgct tgaaagcatc gtggcggaca aaacggctga aattaaaaga ggtcttctcg
    16501 ccggaataaa aggcgaaaaa attaccactg tggagaaaaa agaggacatg gatatcgata
    16561 aaatcctcga tcaaagcggt atcgcactgg ccattgctgc gctactttgc gcatttattg
    16621 gcggtatgcg taaggaaaat cgctggggca ttcgcggcgc gctggttttt ggtatcggca
    16681 tcgtgtgcag cattttgctg atattcctga ttttttcctt cctgaccggc ggttcgctgg
    16741 tttaagcagc ctgaataatg cccgccgggt gatcatcagc gggcattatc gtcagaattt
    16801 ctgttttttc tcctccacct tcactccttg cgtcggtagg ccggtgtcat agtcgcgtac
    16861 cactggcgaa tagccatctt gtgggcgtgg gcgaaacgcc cccatccagc gcggtcgtgc
    16921 atctttacgc cacggacgca gactccattg gtaactgcgg aacggatcgc gaattttgtc
    16981 catgtaatcc aataaggcgt catgcatttt gctgcgaacg tctgcaaaac ggatatcatc
    17041 gatcaggtta tgcatttcat ttgggtcatt acggcgatcg taaagttcat cactggtgaa
    17101 gaggttgagc accagtttaa agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc
    17161 aaagctgtca tgctcaatct cgtagcggtt aaattccacc atcacgccgc gtggctcttt
    17221 cacggcaagg atattttccc ccggcagaat ctctggtttt tcaatatctg ccagcgccat
    17281 cattgtcggc agtaaatcaa tatgactgac tggcgtatcg acctgtcgcc gctccccttg
    17341 cggcgaacgg atgatcagcg gaatacgggt gatgtcgtca tacatcgccg ctcctttact
    17401 gatcagctta tgtgcgccca tcatttcacc gtgatcggag gtataaatga cccacgtatt
    17461 ttcacgttgc tctggcgtta aggcattgat gacacgtccg atttggtcat caacaaagtc
    17521 attgcaggca aaatagagcg gatggtgata aagcccgtca tcaccgactg gcgatggcat
    17581 cgcctgcgcc cataagcggt gatgttccgg tttgttcgcc aggtcatcct gtgatttctc
    17641 gccaagttcg tagtaaaaat cagcgtattt ctctaaatac tccaccggac aggtgaacgg
    17701 gtgatgcggc tcatcatacg aaaccaccat caggaagggt tcctcggcgc gcacgggcta
    17761 ttgcagaaaa tctaccgctc gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat
    17821 atggttcgcc tgtaaatctt cgacgctgtt taggccattg cgccacaggc tgatctcttt
    17881 ttccgtcagt tcgctaagat agttcgcccc atcaaaccag taatcagcat cccactccgg
    17941 cggacactcg ccagtgccga aatagtcatg accgtcgaga tgccatttgc cgatgtaaca
    18001 ggtgtgatag ccggcatcct taaagtagcg ccccatagtg gagatgtttt tgcctggcgc
    18061 gacgttgttg gtccacgggc cggactggtt agcgtagata ccggtgaata gtccggcccg
    18121 tgcaggcgta caaaccggtg aacaagtgta ggcggaatta aagcgaatac cttccgccgc
    18181 cagactatca atattttgcg tattcagcgg tttaccgcta tagcaaccga ccatattggt
    18241 ggcctgggta tcggtcaatt ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg
    18301 ttacggctgg gtcgtgacga tgccaccagc tgtcaaaaat caggtagaca atcactacgg
    18361 cagcgatgaa ataactgatc atcgctaacc agcgcgtacc gtagccgccg aattcagcca
    18421 gcccgacata gacgccaatc atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt
    18481 tccacggttt catgtccacc gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt
    18541 tgataaaacc gatcaccagc atcacgacca cgttgatgca gaacgtacag gccagcacat
    18601 agaggaaatg gaagtcgaac ttcaccagat agttgatggt gatgtagctg attatgccaa
    18661 tccccatcgc cacttttgcc gccagcgccg ggatgcgcgg aaagaaaaag cccataatga
    18721 tgatggtaac cagcggcacg ttgtaaatgc cgttgagctg tttcatccag ctatacagcc
    18781 cctgcggcgc gttcgcgatc cacggcgcta ccaaaaccga aacgatagcg ataaagaaac
    18841 caaatttgcg cccgacggtg accagctgct gcggctcggc attctggtta atgatgcgac
    18901 ggtaaatacc catactgaat aatgtactgg cgctattcag aaagccgttg aaggtactga
    18961 tcaccgcacc aaataacacc gcgccgaaga aaccctccag tggcactggc agaacgttat
    19021 tgaccagcgt cgggtaggcc atgtcggctt tcggtagatc ctgatacaga tgaaacgcaa
    19081 tcaaccctgg cagcaccagt accagcgggt cgagcatttt cagcaccgcc gttaacagcg
    19141 cccccttttg cccttccgcc aggctttttg acgccagcgt gcgttgcacg atgccctgat
    19201 ttgtacacca gtaaaaggtg ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca
    19261 agggatcggt cgggccaccg actgagttta atttctcggc gtgaacggtg gtaatttgct
    19321 caacgccctg cataaagctg cccttgccta tcgcgatcag gccaaatacc ggcaccatca
    19381 acccgccgat aaccagccca ataccgttga tggagtccgc cactgccatt gcgcgcagtc
    19441 cgccgatcac cgcatacaaa attcccgcca gaccaagcaa aattaccagt agccagatag
    19501 ccgcaccgtg agaaatctgt agcgattccc cgacgtgaaa caggctgttg agcgccaacg
    19561 cgccggagta gagaacaatc ggcagaaagc agacgccggt ggcaatgagg aagcagaagt
    19621 cgatgataat acgcgtcgtt ttatcataac gttcctccag aaaatcgggg atggtggcaa
    19681 tgccgcgctt cagatagcgc ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg
    19741 aagtcacttc ccagcccatc accgacatgc cgcttttgta ggcctgcccg gaaagaccaa
    19801 ccagttgttc cgtggaaagg ttggttaaca ttaacgaagc ggcaataacc ggcgctttta
    19861 gtgaacgtcc ggcaagaaaa tagccttgtt gcgatccggt atctgttttg cgaaccttcc
    19921 accaggtgat caccgccacc agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat
    19981 tcatctcata gcccttattt atgtttatgt cccggacgca tgaatatagg gagaggtaaa
    20041 atgcgtcggg tcaggcagtc agattcttaa aatacaaacg tcgtatccct gaacggattg
    20101 ttttctgtta agttcaggtt gtgagcataa ttcgccggag ggatattaaa atgaatggaa
    20161 aattgcaaag ttcagatgta aaaaacgaaa ctccgtacaa tattccctta ttgattaacg
    20221 aaaatgtgat ctccagcgga atttctctga tctcgctctg gcatacctac gccgacgagc
    20281 attaccgggt gatctggccg cgggacaaga agaaaccgct tatcgccaac tcatgggttg
    20341 cggtgtatac cgtacaagga tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc
    20401 tgcatagcaa ctgtattata tttttaaagc caatggatat tcactcttat cactgtgaag
    20461 gtttagtctg ggaacagtac tggatggaat ttacccccac cagtatgatg gatatttccg
    20521 ttggtcagca aagcgttatt tataatggcg aaatttataa tcaggaactc accgaagttg
    20581 ctgagttaat aacttcacca gaagcaataa aaaataatct ggcagtcgct tttctgacga
    20641 aaattattta tcagtggatt tgtcttatgt ccgcagatgg taaaaaagat ccacaacggc
    20701 ggcaaattga aaaattaatt gccactttac atgccagtct gcaacaacgc tggagcgtag
    20761 ctgatatggc tgccacgatc ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct
    20821 ataccggcaa gacgccgaaa gaatattacc tcgatgcgcg tctggatctg gcgctatcgc
    20881 tattaaagca acaaggaaac tcggttggcg aagtcgctga tacgctcaac ttcttcgact
    20941 cctttcattt cagcaaagcc tttaaacata aatttggtta tgcgccgtca gccgtgctaa
    21001 agaatacgga ccagcaccca acggatgcca gtccacacaa ttaacgtaac tctggccagt
    21061 acgctttgtt ggcctccagc aactcatcca gaatagcttt ggcgaccgag gcgctcggta
    21121 ccgttttcga caacgtaatc gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt
    21181 ccacgaccag tttttccacc gccacttgct gactcattaa ccctttctga aactgcggga
    21241 tatcgccgac caccagtggt tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg
    21301 cagcccaaca atttgcgcca tccggctttc aataccgatt ggcatgtcac agatgttgag
    21361 gattttcgca ttcgggcgca gacgacgcgt ggcttctgcg acaattgctg ccgggttgga
    21421 gtagttgagc atctaggcgt ttggtgaata tttttccata taatccacca gttccaggac
    21481 gccgccaatg gaacgcatgc cgtacgctat tccgccgggt ccgcaagttt cctgaccaac
    21541 aacaccgtgg cgcagtggga ttttttcatc cagttcgcgc atcgggtact tgccgacgcg
    21601 gatgtgcgcc atgacaaaat caacgtcgct gaacgccact tcaggatcgg tggtgtaact
    21661 aaacgcaatg tccggcgctt tttctttaag gatgacttta caggcttcgg caatcacttc
    21721 ctgccgcgcg ccatcgttat cataaaattt cagtgcacga agcgggaaac ggtcctgatt
    21781 cgccaggagc atcaacacga tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac
    21841 tgagaatttg gtcattatac tgcctccgta atggcaacat tttctgctga ttgatgagaa
    21901 ttaattaagc tatcgagctg ttcacgcaat tgggatacat gcagaccaat gatcacctga
    21961 atggcatcgc cactacggaa gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc
    22021 agcgtttgtg acatgtcatg cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg
    22081 gagatattgc cgaccccgcc cagggcttgc aggataccgg cagcctgatc cagctctttt
    22141 tttggctctg cagcggtggt ttggcctcgc gaggctttgt attcggcttt tgagtagagt
    22201 ttcacttccg catcttcacg tcccggcgtg cacatgttga actgcagaat cagggtgcgg
    22261 aaaaccacga agtacagcag ggtaaagcac aacccaatgg cgatttgggt cagcatcata
    22321 tccgcgtggt tgctgaacat cgggatccag ttttgcggta aaacctggtc aatcagacct
    22381 ccgcccatgt tgcccaccac accaaagaga tacattacgg tcgacattga ggccgccagc
    22441 acggcgtgta ccgcaaacag caacggtgaa atgaacagga aggtaaattc cagcggttcg
    22501 gtaattccca ccagcatggc ggttaaggtt gcgggaatca gcaagcccgc cacttttacc
    22561 cgattttccg gtgcggcagt gaagtacatc gctaaagaaa tgcccacggc accaaagatt
    22621 tttgagttac cgtgcagggc aaaaccgcct tccgggaaca acgatttcag tggctcggcg
    22681 ctcagactga actcttgcag atgctgcgcc cagtacatct gaatgccgcc ttcaacagct
    22741 gccggaccaa agataaacgg tccgtagatg aagtggtgta aaccggttgg gatcagaata
    22801 cgttcgagga aggtgtaaac ccacacccca agcgcacccg ccgaacgcag gaacgcttgc
    22861 agagattcaa tccccatttg tacttttggc cagccgagca atgtcagcca ggcacagggg
    22921 atcatcacca ggaaggcgat aatcaccaca taagacgtcc cctggaaaat gccgagaaaa
    22981 accggcagtt ttttatcgaa cagacggtta tgcagcgccg tcacaatgcc ggaaatgata
    23041 attgcgccga taatgctggt atcgagggtt ttaatcccgg ccatcattgt cagaccgcta
    23101 cctgccaccg cgtcctgagt gaaatcgacg ccgaagtagc ttccccaggt cattcccatc
    23161 gcattgatga aatagttcca ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc
    23221 tgcgcttgct tagcaaggcc aatgggtaaa ccgacagcaa aaatcagcgg catattacgg
    23281 aataccgtcc aaccgccctc ttcaataatg tgtacgattt gcgcgaataa actgttcgga
    23341 tcggtcagtg attccccgac aaacatcggg ttttgcagca agatggcaag acccaccaca
    23401 atcccggcga agggaaacag cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt
    23461 tgactgagca tttccacatc ctcttttctc aattctgtaa gtaggaagcc tgattggtta
    23521 tgcggcagac ctggagcgag gataggaaga tgcgatcggc agaacatgac gacccaatgg
    23581 gattcgtgat cgctttcatg cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa
    23641 gtagacatct gtgagcacag gtacacagca tcgaacacat ctttaaaaaa aagatttttt
    23701 tcaatcgatt aagcagaact tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc
    23761 tagagatgat ctacaaaagc attgcggagc ggttaagaat tcgacttaat tccgcggatt
    23821 tcacgctaaa cagccttctt cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc
    23881 ggatgactat ccgtaaagcc attgacctgc tggtagcgtg ggggctggtg gtccgccgcc
    23941 acggcagcgg cacttacctg gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg
    24001 gactggtgga ggtgttaaaa cggcagggaa aaacggtcac cagccaggtg ctgatttttg
    24061 aaatcatgcc tgcgcctccg gccattgcca gccagttacg gattcaaatc aacgagcaga
    24121 tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc gctaatgctg gaagacagct
    24181 atatgccggt aaaactgttc cgtaaccttt cgctgcaaca tctggaaggg tcgaagtttg
    24241 aatatattga acaagagtgc gggattttga ttggcggtaa ttatgaaagc ctgatgccgg
    24301 tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc
    24361 ggatcacctc gttgtcatat agcgagagcg gggagttttt gaattattca gtgatgttca
    24421 gaaatgccag cgaatactag gtggagtacc atttacggcg actccacccg gaaaaaagtt
    24481 aaccgatact ccagaagagc accgccagta attggggggt gataatgcgc aggaacatca
    24541 ctaacggata gacagtggcg taagagagcg ccgccgcacc actggttgga tgaagattat
    24601 tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg cacatggtca
    24661 ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc ggaacggcgg
    24721 tgatcagggc accataacca atccagctta gcccttcgcc attgaccagg gtattcacaa
    24781 aatccccacc agatttcaga ccaacgaccg agaggaacag cacgatcccc agctcccgca
    24841 gcgcgaggtt ggcacttggc ggcataaacc agtacagctt gccgatactg ccgatacgcc
    24901 cgaggatcaa cgccataatc agcggaccgc ccgccagccc cagtttcaac gcggccggga
    24961 atcctggcac aaagacggga atagaaccta acaatacgcc aagcccgatg ccaataaaca
    25021 ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg
    25081 catcaattgc ggacggacgc cccaccaggt tgaggatatc gccgaactgc aggctgatat
    25141 cgccactggc gaccagttcg accccggcac ggttcaggcg cgagataaca acgtcatagc
    25201 gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac gttttcattg gtgaccacca
    25261 cacgctcgac gcgcaaatca gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa
    25321 tcaccagttg cgcattatgt aaatccgctg gctgccccac cagatgcagc aaatcgccca
    25381 gttggataat ggtatctggc gaaggtactt ttagggtttc ttctcgtttc agacgcgagc
    25441 agataatttt gtcgccgttg agaatcggta catctttaat ggctaaatca tgcaggttag
    25501 ggttctcaac gcgaatattg atagtcttga tcagcgcgcc gccattggtg cgtgaagact
    25561 cgtgctgctg agcttctgtc tcgacattga cgcggaaaat aacccgcaac atccacatgg
    25621 tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc gtaactcatc cccatctgat
    25681 cgaccatttc cattggtgta cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg
    25741 gcgtattggt taccgcaccg gagaaaatcc ccagcactac cggcagtgga atatcaaaca
    25801 gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca gcaaacaggt
    25861 tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc tgaatcccga
    25921 tggtataaac gaacaggatc aggccaaatt cctgaataac atgcagcata tcgctactta
    25981 atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccaaac agcacgccgc
    26041 caatacctaa tcctatgccg cgaaatttga cgttgccgat aaacaaaccg acgactgcca
    26101 ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc tcttgagata
    26161 acattttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct tatagcgcac
    26221 attatcacgg gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa cgcattgttg
    26281 aagaagaaag acaaaaaaag ccccaccaga atggcggggc aaagagaata gctagttagc
    26341 tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat
    26401 tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag acttccatat
    26461 tttcagccag cgtaaaactc aggctaaatg gctgattcat aagcccttga tgcagccatt
    26521 ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgtg ccttccagtt
    26581 gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat
    26641 cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg ttttgcagtg
    26701 cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa tcgaagttac
    26761 gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa gattcgcata
    26821 tgctccctga cggcgagcat ggagatgtca ggccgcgcca ggcggcctta gggaattagt
    26881 tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga tcgatataca
    26941 gcaaaccatt taccaggtta gccccacgaa catgaatgtt ctcagctaac tggaatttgc
    27001 gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt tgttcgtcgg
    27061 cgtgagcacc tttcaccacc agcagattat cctgggcggt aatttccagt tcgctctcag
    27121 caaaaccagc cacagcgata gcaatgcggt aatggttttc gtctaccagt tcaacgttat
    27181 acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta aacaaacggt
    27241 caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata atcaatagct
    27301 cctgaaatca gcgagaatgt aagaccttcc acaatggaca ggtcaggtag ccagaacacc
    27361 catcaggcgc gtcctcatcg actacgatgt aaaatgggtc tggaaatgaa cttttcaagc
    27421 cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag ggggcagcac
    27481 gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa
    27541 gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt attatgataa
    27601 gaaatgtgtt gttagcgttc atgatatgca gcggaatgac attactcgga ggatgctcca
    27661 gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc cggcacccgc gccagcgcca
    27721 ccatgattgg cgacgatgaa accaactggg gcactaagtc gctggcgatt ctcgatatgc
    27781 cgtttaccgc tgtaatggac acgcttttac tgccgtggga tgtgttccgc aaagacagct
    27841 cggtgcgctc gcgcgttgaa aaaagcgaag ccaacgcgca agccaccaat gcggttattc
    27901 caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc acagctggcc
    27961 accttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt ccgcagaagg
    28021 tggattggcg tggttttcgg ttaaatactc aacctcgccg ctttgcgcat gggcacaaac
    28081 aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt taaatgcaga
    28141 ctgaatatcc gttttgttat gggttaactt gcgcggttcg ccgccctgtg gcgagataag
    28201 ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg taccctgcgg
    28261 attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc gatgatgggt
    28321 aaaggttaaa cgtcgctgca cgacgccacg cggtggcgcg ggtagggttg tttccgttcc
    28381 acttaacggc gcatcacctg ccgatttcca gctagcttca tcttgcggta actcaacgat
    28441 aaatagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc
    28501 gtgatttcct acccatcctt cttcataagc acgattgatt tcatcactgc caggctgcgg
    28561 cgtgggcgtg gttttactca ccagcacaca ccagtggcta ccgctgtatt cacgcggatg
    28621 ttgcttttgt acgttgaccg ggccaaacgg tgcagcaacc ccgacgtttc gcaaatccag
    28681 cgccggatcg agttgatgca ttacatggtc gttataggta aagctccccc tttcaccgtt
    28741 cgggctaaag acatgcacat ggctgccacc gcgcagcgct cctggcgtat acggcgcggt
    28801 aatatccatt gcatcgagat tgctcacctt gccgccttca gcaatgactc cgcgtcgatg
    28861 atggaagtcg taatgccatg tttcatcagg attttcaggg ccgtgaataa agacatattt
    28921 ctctgacttc ggatgaacgg tcaccacgcc gacgtgtgcg ccctgtgacg cgcgatagat
    28981 aacctcgacc tcgcctgtat ggatattcac acgctcaatg gtctcgccgg taaacgacgc
    29041 cccagaagga cgcacgtcaa ataccagcca ctggctgtcg ggcgtccagg tattggtatt
    29101 tgtattggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
    29161 ggcgcggtcc tgatacaaaa cggttcccgc catcatactt cacaaggact tcactttcag
    29221 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
    29281 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
    29341 ggtgatcgcg ctggataaaa agcaccagtg cggtactgaa ggtttcagca aaccttgcgg
    29401 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatggactga cgcttcctgt
    29461 cgatgtgatc gccaatccac agattttcag cgtcaaaact gtcgacgtcg ccgcatcgct
    29521 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
    29581 gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc gaaaaatctg
    29641 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc aacatattac
    29701 cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
    29761 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
    29821 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
    29881 caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
    29941 cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
    30001 tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg gtaaggacaa
    30061 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    30121 ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc cggagaagag
    30181 caacgccgct tactggcgcg ccacacacaa actgcgttta aaactcttcg gcaagatagt
    30241 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    30301 gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca gcaggatgta
    30361 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg cgaaaccgta
    30421 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    30481 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
    30541 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
    30601 catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    30661 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
    30721 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    30781 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    30841 aaaccacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca gtttacgatg
    30901 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggcgc
    30961 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatacc
    31021 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    31081 accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    31141 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
    31201 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    31261 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    31321 ttggaataaa gtcgccactg accaaccaaa tatcgcaata aaataagtca cgcgagaacc
    31381 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    31441 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    31501 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    31561 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    31621 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    31681 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    31741 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
    31801 ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt cgttaacggt
    31861 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    31921 gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt ataggaaacg
    31981 aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg cggcgcaagg
    32041 gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat accgcctgca
    32101 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    32161 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    32221 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    32281 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    32341 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    32401 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    32461 tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg acgatcgccg
    32521 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagact
    32581 ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc ggcgatggcg
    32641 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtcg
    32701 ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    32761 accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    32821 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    32881 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcctg ctgcgcggtg
    32941 cgttctacgg actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct
    33001 gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg
    33061 tccggtggta ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga
    33121 aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg
    33181 cagccggggc aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata
    33241 ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc
    33301 agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg
    33361 ctctgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc
    33421 accgcgccaa catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc
    33481 gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc
    33541 gcccgccacc gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt
    33601 cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact
    33661 ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag
    33721 tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt
    33781 aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc
    33841 tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg
    33901 aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac
    33961 ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat
    34021 cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc
    34081 aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc
    34141 aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc
    34201 acgccagtcg gtcgtgactt ctgctaacac tgcagccgga gattttccgt tcaggcgcgt
    34261 gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg
    34321 cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac
    34381 cttcgtgtcg agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga
    34441 tgccgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca
    34501 tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt
    34561 tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct
    34621 tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc
    34681 tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc
    34741 agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc
    34801 cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg
    34861 cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg
    34921 atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccgcaag tggcgagcca
    34981 ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta
    35041 ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat
    35101 gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa
    35161 tgtagtacaa acttatattg ttgcgctaca atttagatca aaaaaagaac aacacataaa
    35221 aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa
    35281 agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat
    35341 tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt
    35401 atctcgtatg ctctggtttc ccataccgca aagggtagtt caggaaagta tcaatcgcag
    35461 tcagacacca tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt
    35521 tttgtagggg aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg
    35581 ctcaatgatc cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt
    35641 ttaaatgcgg ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac
    35701 gaagaagaaa aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc
    35761 aggtcactgg aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca
    35821 aaggtgatgg cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga
    35881 ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacact ggacatcatt
    35941 actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa
    36001 acgcgtttat gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa
    36061 cgccacgcca tcttcaaggt tggattttgt gacaaagttc gccacttctt tcaccgaagg
    36121 aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc
    36181 ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt
    36241 cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt
    36301 cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat
    36361 ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc
    36421 ttcgcagaac accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat
    36481 atcgcggtta gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc
    36541 gcgagagagt ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt
    36601 gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg
    36661 ctgttccata tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt
    36721 tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga
    36781 aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc
    36841 catgagttcc caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc
    36901 agtacaacac ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag
    36961 cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt
    37021 gctgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt
    37081 aaactggaag ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
    37141 gtgccgagcg ccgactccag ttgctcgccg ctaactgatt gatttctctg ttgattactc
    37201 aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
    37261 atgccaccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc
    37321 agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt
    37381 gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg
    37441 cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttac
    37501 ccggcctaca aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg
    37561 tagcgcatca ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca
    37621 gggcgttctc ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga
    37681 acaactggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg
    37741 ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
    37801 ggatggagag gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg
    37861 ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca
    37921 gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca
    37981 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt
    38041 gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca
    38101 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt
    38161 ccgtcaacgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
    38221 tgcggttgat cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc
    38281 cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga
    38341 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca
    38401 ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct
    38461 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
    38521 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
    38581 cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
    38641 gcgctttctc gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
    38701 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa
    38761 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg
    38821 cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat
    38881 cgataatttt gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca
    38941 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
    39001 tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt
    39061 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
    39121 tgtaggcatt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt
    39181 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
    39241 gcagcgccac ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt
    39301 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt
    39361 ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga
    39421 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
    39481 tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
    39541 taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
    39601 cgcgctgaat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
    39661 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
    39721 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
    39781 cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt
    39841 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
    39901 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
    39961 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
    40021 gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa
    40081 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
    40141 tgcgtcgctt ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca
    40201 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc
    40261 gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc
    40321 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt
    40381 agctggcgat cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc
    40441 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca
    40501 tccatatcgg ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc
    40561 agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc
    40621 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccaggt
    40681 tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt
    40741 aaagtaaacc cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact
    40801 ttatgcccgt ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag
    40861 ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc
    40921 tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta
    40981 tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag
    41041 ccgggagata aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg
    41101 gtgagggaca ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat
    41161 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
    41221 gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc
    41281 tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt
    41341 tcagctggtt ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg
    41401 ccgcgcgagc aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt
    41461 tcttcggcag aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa
    41521 taaagtcgcc aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt
    41581 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat
    41641 ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg
    41701 ccacggtgcg cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa
    41761 catcctgatg cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg
    41821 gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca
    41881 gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct
    41941 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
    42001 gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca
    42061 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
    42121 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
    42181 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
    42241 tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag
    42301 gcatgaagca ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg
    42361 tggttagtca gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg
    42421 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
    42481 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca
    42541 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc
    42601 agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
    42661 tcacccggca aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt
    42721 tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga
    42781 tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta
    42841 ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca
    42901 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
    42961 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
    43021 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg
    43081 tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg
    43141 ttatccgcca cctggcaagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
    43201 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg
    43261 acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
    43321 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
    43381 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
    43441 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
    43501 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
    43561 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
    43621 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
    43681 cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc
    43741 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
    43801 tcttctgttt ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt
    43861 gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac
    43921 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
    43981 ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc
    44041 gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
    44101 ccggtgtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
    44161 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
    44221 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
    44281 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
    44341 gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt
    44401 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
    44461 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
    44521 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
    44581 cgcctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
    44641 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
    44701 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
    44761 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
    44821 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
    44881 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
    44941 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
    45001 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
    45061 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
    45121 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
    45181 caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa
    45241 ctctacccag ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag
    45301 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
    45361 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
    45421 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
    45481 cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc
    45541 gtttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
    45601 cttcgcactg cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa
    45661 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
    45721 ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
    45781 caacttctat accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc
    45841 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
    45901 aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
    45961 gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg
    46021 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
    46081 gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc
    46141 aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta
    46201 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
    46261 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
    46321 actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg
    46381 cgtaacgatg ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca
    46441 gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg
    46501 tctggtactg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    46561 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    46621 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    46681 gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    46741 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    46801 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    46861 tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc
    46921 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    46981 gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc
    47041 tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc
    47101 gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct
    47161 gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat
    47221 ttacgtcgaa gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa
    47281 aattgaagcc cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg
    47341 tcagggtagt ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg
    47401 taaatcgagc ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc
    47461 cggaaccacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat
    47521 catcgacact gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg
    47581 cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac
    47641 agacgccgtt gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact
    47701 gccgatcacc gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga
    47761 agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct
    47821 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc
    47881 gcgccgtcgc cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc
    47941 gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca
    48001 gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc
    48061 cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg
    48121 cttcgcttca ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaattct
    48181 tacgtaattt ataatcttta aaaaaagcat ttaatactgc tccccgaacg attgtgattc
    48241 gattcacatt taaacaattt cagaatagac aaaaatt
//
LOCUS       DAJTRW010000022        47945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRW010000022 DAJTRW010000000
VERSION     DAJTRW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO326_001355"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO326_001355"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5965794.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO326_001356"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO326_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5965795.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO326_001357"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO326_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5965796.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO326_001358"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO326_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5965797.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO326_001359"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO326_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5965798.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO326_001360"
     CDS             5377..5835
                     /locus_tag="OO326_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5965799.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO326_001361"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO326_001361"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5965800.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO326_001362"
     tRNA            6887..6971
                     /locus_tag="OO326_001362"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO326_001363"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO326_001363"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO326_001364"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO326_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5965801.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO326_001365"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO326_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5965802.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO326_001366"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO326_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5965803.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO326_001367"
     CDS             complement(10252..13995)
                     /locus_tag="OO326_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5965804.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO326_001368"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO326_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5965805.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO326_001369"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO326_001369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5965806.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO326_001370"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO326_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5965807.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO326_001371"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO326_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5965808.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO326_001372"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO326_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5965809.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO326_001373"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO326_001373"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5965810.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO326_001374"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO326_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5965811.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO326_001375"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO326_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5965812.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO326_001376"
     CDS             23392..23499
                     /locus_tag="OO326_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5965813.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO326_001377"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO326_001377"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5965814.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO326_001378"
     CDS             complement(24538..25347)
                     /locus_tag="OO326_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5965815.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPPAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO326_001379"
     CDS             complement(25351..25743)
                     /locus_tag="OO326_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5965816.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO326_001380"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO326_001380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5965817.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO326_001381"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO326_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5965818.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO326_001382"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO326_001382"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5965819.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO326_001383"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO326_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5965820.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO326_001384"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO326_001384"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5965821.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO326_001385"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO326_001385"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5965822.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO326_001386"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO326_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5965823.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO326_001387"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO326_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5965824.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO326_001388"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO326_001388"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5965825.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO326_001389"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO326_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5965826.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO326_001390"
     CDS             36790..39657
                     /locus_tag="OO326_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5965827.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO326_001391"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO326_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5965828.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO326_001392"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO326_001392"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5965829.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO326_001393"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO326_001393"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5965830.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO326_001394"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO326_001394"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5965831.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO326_001395"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO326_001395"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5965832.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO326_001396"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO326_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12034 a        12343 c        11743 g        11825 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg gcgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgca
//
LOCUS       DAJTRW010000023        47504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRW010000023 DAJTRW010000000
VERSION     DAJTRW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="OO326_001397"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="OO326_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO326_001398"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO326_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5965833.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO326_001399"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO326_001399"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5965834.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO326_001400"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO326_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5965835.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO326_001401"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO326_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5965836.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO326_001402"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO326_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5965837.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO326_001403"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO326_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5965838.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO326_001404"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO326_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5965839.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO326_001405"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO326_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5965840.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO326_001406"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO326_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5965841.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO326_001407"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO326_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5965842.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO326_001408"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO326_001408"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5965843.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="OO326_001409"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="OO326_001409"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5965844.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="OO326_001410"
     CDS             11405..12259
                     /locus_tag="OO326_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5965845.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="OO326_001411"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="OO326_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5965846.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="OO326_001412"
     CDS             complement(13722..14861)
                     /locus_tag="OO326_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5965847.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO326_001413"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO326_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5965848.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="OO326_001414"
     CDS             16047..17408
                     /locus_tag="OO326_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5965849.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQCLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="OO326_001415"
     CDS             17405..17923
                     /locus_tag="OO326_001415"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5965850.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="OO326_001416"
     CDS             17923..18243
                     /locus_tag="OO326_001416"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5965851.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="OO326_001417"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="OO326_001417"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5965852.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20274
                     /locus_tag="OO326_001418"
                     /pseudo
     CDS             19062..20274
                     /locus_tag="OO326_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20371..20793
                     /locus_tag="OO326_001419"
     CDS             20371..20793
                     /locus_tag="OO326_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5965853.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20841..21713)
                     /locus_tag="OO326_001420"
     CDS             complement(20841..21713)
                     /locus_tag="OO326_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5965854.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21725..22819)
                     /locus_tag="OO326_001421"
     CDS             complement(21725..22819)
                     /locus_tag="OO326_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5965855.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22852..23850)
                     /locus_tag="OO326_001422"
     CDS             complement(22852..23850)
                     /locus_tag="OO326_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5965856.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23875..25386)
                     /locus_tag="OO326_001423"
     CDS             complement(23875..25386)
                     /locus_tag="OO326_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5965857.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25409..26391)
                     /locus_tag="OO326_001424"
                     /pseudo
     CDS             complement(25409..26391)
                     /locus_tag="OO326_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26488..29589)
                     /locus_tag="OO326_001425"
     CDS             complement(26488..29589)
                     /locus_tag="OO326_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5965858.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29596..29769)
                     /locus_tag="OO326_001426"
     CDS             complement(29596..29769)
                     /locus_tag="OO326_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965859.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29887..31080
                     /locus_tag="OO326_001427"
     CDS             29887..31080
                     /locus_tag="OO326_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5965860.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31278..32531)
                     /gene="glyA"
                     /locus_tag="OO326_001428"
     CDS             complement(31278..32531)
                     /gene="glyA"
                     /locus_tag="OO326_001428"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5965861.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLELGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32858..34048
                     /gene="hmpA"
                     /locus_tag="OO326_001429"
     CDS             32858..34048
                     /gene="hmpA"
                     /locus_tag="OO326_001429"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5965862.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34093..34431)
                     /gene="glnB"
                     /locus_tag="OO326_001430"
     CDS             complement(34093..34431)
                     /gene="glnB"
                     /locus_tag="OO326_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5965863.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34492..35826)
                     /gene="glrR"
                     /locus_tag="OO326_001431"
     CDS             complement(34492..35826)
                     /gene="glrR"
                     /locus_tag="OO326_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5965864.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35816..36529)
                     /gene="qseG"
                     /locus_tag="OO326_001432"
     CDS             complement(35816..36529)
                     /gene="qseG"
                     /locus_tag="OO326_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5965865.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36694..38121)
                     /gene="qseE"
                     /locus_tag="OO326_001433"
     CDS             complement(36694..38121)
                     /gene="qseE"
                     /locus_tag="OO326_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5965866.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38679..42566)
                     /gene="purL"
                     /locus_tag="OO326_001434"
                     /gene_synonym="purI"
     CDS             complement(38679..42566)
                     /gene="purL"
                     /locus_tag="OO326_001434"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5965867.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42825..44381
                     /gene="mltF"
                     /locus_tag="OO326_001435"
     CDS             42825..44381
                     /gene="mltF"
                     /locus_tag="OO326_001435"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5965868.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44378..44914)
                     /gene="tadA"
                     /locus_tag="OO326_001436"
     CDS             complement(44378..44914)
                     /gene="tadA"
                     /locus_tag="OO326_001436"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5965869.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44939..45574)
                     /gene="yfhb"
                     /locus_tag="OO326_001437"
     CDS             complement(44939..45574)
                     /gene="yfhb"
                     /locus_tag="OO326_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5965870.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45783..46631
                     /locus_tag="OO326_001438"
     CDS             45783..46631
                     /locus_tag="OO326_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5965871.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46970..47504)
                     /locus_tag="OO326_001439"
                     /pseudo
     CDS             complement(46970..47504)
                     /locus_tag="OO326_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11326 a        13017 c        11951 g        11210 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaatgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat gggccgtaat
    19621 gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg tgtgcgcatt
    19681 ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact gacgctgcaa
    19741 agtggggaaa cgcttcaggc tgatgtggtg atttacggta ttggtatcag cgccaacgag
    19801 caactggctc gcgaggccaa ccttgatact gccaatggca ttgtcattga tgaggcttgc
    19861 cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg tcttgataat
    19921 ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca aattgccgct
    19981 gccgcaatgt tagggctacc gctaccgcta ctgccgccgc cgtggttctg gagcgatcag
    20041 tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct ttgtcgtggc
    20101 aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct tatcggtgcg
    20161 gtcacgctga atcagggccg tgagattcgc ccaattcgca aatggatcca gagcggcaaa
    20221 acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact gtaatcagga
    20281 taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg tattacgttt
    20341 aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt tgattttgga
    20401 gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat tttcattatt
    20461 tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc ctcgttgggc
    20521 gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc cgccgcgata
    20581 ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt ctacacgctg
    20641 ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt tgggccaaat
    20701 atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt ggcaattacc
    20761 ggaccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg gcgtgaacgc
    20821 cttatccggc attttataaa ttacagccac tctacccgca acgacatctc tgaggttgtt
    20881 gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg ctcatccggc
    20941 gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc aaacgcaggg
    21001 tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat gatggcataa
    21061 ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc attgttcacc
    21121 cactgacgcg gcaacggcgc gagctggtta aaatccagtt cctgcggtag ctgctcgcaa
    21181 aactcacccg ccagccactg ctcccgttcc agccagtaac cgctcgcctg cgcctgaatc
    21241 cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg taccaaatgg cagcgtctct
    21301 gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa atgaaacgcc
    21361 tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca caaactatca
    21421 tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag atagtgcgca
    21481 tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa acggttgccg
    21541 ctcacccggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc cacaccgctt
    21601 tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat tacgccgccc
    21661 tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt catccgctaa
    21721 ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc atcagcacat
    21781 aggcatcacc tgcctgttct agcgagaagc gatgggtaat ggcgttacgc ggccacagct
    21841 tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc acccaggagc
    21901 cgataatccg ccgttgatgg tgcatcagat cagcgctgac ctcgaattcc acttttccgg
    21961 tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc agcaagcgac
    22021 ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta agttcagcaa
    22081 ttatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcccc cctaactgtt
    22141 ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt gcaccgcgac
    22201 ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc agcacgttat
    22261 cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg ccgcaactga
    22321 taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct ttttcttccg
    22381 ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct tttcctttgc
    22441 cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag ccagaaatgt
    22501 gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc atcgccacaa
    22561 tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta tcgggtgccg
    22621 ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa atcccggagg
    22681 atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact tcccgcagat
    22741 cgacggttga atttcctagt aaataagcag ccagcatcgt tttcataaat ccctcattaa
    22801 caatacgatt aattttcatc cctgcagcac ccgcgccagg ggcgttaagg tttagcgttt
    22861 cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca cgatgatgac
    22921 gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac cgttaccgat
    22981 tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac cgccggaaag
    23041 tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc
    23101 tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg gtcaccgccg ctaataatcc
    23161 cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca actgcgccgc
    23221 cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc ggctaatgaa
    23281 cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac cgagaaattg
    23341 tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg cgttggtcat
    23401 aaacagcccc attccgcgca gagcgctcca cagccccagt gtggcaacga aacttggtac
    23461 gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcgccta acagcaacac
    23521 cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca ggcacaccga
    23581 gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa taatcagcgt
    23641 catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca tctggttatt
    23701 caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg caatcaccac
    23761 cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga cgctcttgcc
    23821 ctgcggcaat ggtaatgacg aagcatacat cttcaggttc ctcggtaaaa ttcatcagtg
    23881 cacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa actcctgcga
    23941 gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaatt cctccacttc
    24001 actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa tacggtaaat
    24061 ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca aaatctggct
    24121 ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag aaagcgtgcc
    24181 gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct cttcggtcag
    24241 gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc gattggtcag
    24301 cactgtattt tcgtcaacgc ctagccaggg aatgatcccc gcttctttgc ggttttctgg
    24361 cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga ttttctcgcc
    24421 gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct tcagcaattc
    24481 actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac gtagcgtaaa
    24541 gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta cggcctgatc
    24601 cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga tcacgcccta gcattagcga
    24661 cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct gaccatcgcg
    24721 cataacggtg gcataggagg caatgcggcg gatttcttcc atccggtggc tgacataaat
    24781 caccgccacg cccagtgccg acatcttttt caccgcactg atcaccagtt caacttctgc
    24841 actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc ccttcatcac
    24901 ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc gttgaaacaa gttgttcagg
    24961 actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca tttgcaggta
    25021 atcaatcatg ccgttgcggc gaggccattg accaaggcag aggttttctg ccaccgtcag
    25081 cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag cggcacggcg
    25141 agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat caccgctatc
    25201 cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc cgtttttacc
    25261 taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca gcgcaacgac
    25321 gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct ctgttgccgt
    25381 gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc gttgccagcc
    25441 actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc actttttcac
    25501 cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg cccattttct
    25561 taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc tgagcaattt
    25621 cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga ttacgtaccg
    25681 cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg agatccggcg
    25741 tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc ccttcctggt
    25801 tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat cctttacgtc
    25861 gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc tggtcaattt
    25921 tattggcaat aaaataatcg gcggcagcgt tacccagttt ttaccaaatt ccagtggatc
    25981 gccgaccaga tacgccgaga catatttatc gacacccttt tgattaatac aggtgttgta
    26041 gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac tgccattttc
    26101 agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat caacaaaggt
    26161 actttctttc gaaatatccc cctgggcgtt agtttcaatt aactgtacct gtactgaaga
    26221 atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct gggtatcaag
    26281 gtatattgca ccaatggtca tttctttatc cgcagccctg gcaaatagtg cgctacctaa
    26341 tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca tagtgcctcc
    26401 tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg
    26461 ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac
    26521 agtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc
    26581 agactcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca
    26641 tttttcttta tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag gttgtgaaac
    26701 agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc
    26761 actaaacagt tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca
    26821 gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc
    26881 ggtcgccgcc agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca
    26941 tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa
    27001 attctccgga taatacagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc
    27061 atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc
    27121 ttccccgcct tcccacgggt ggaatttacg cgtggcgaga atgtccgccg ctttgtctgc
    27181 ctgacccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc
    27241 aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cacttaactt
    27301 atccagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt
    27361 atcaagataa cgcgcggcca gctcataatc gtgtttctta ttccacgcat ggatcgctaa
    27421 cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc ataaggcaat
    27481 ggctttgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca
    27541 acactcttca atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa
    27601 ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa
    27661 gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc
    27721 cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcagag cgttaacgtc
    27781 gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac catcgaacca
    27841 gttcagccac cacagagtgg cgttcagcgg ataatcacgc agcagtttct cgcgctgcaa
    27901 acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaagc aaagcacttc
    27961 ctgattggtt gggcaggcac gaagactttg ttggcaaaaa tccagacccg cgtcgaagtt
    28021 atcattacgc gccgccagtc gtgccagacc ataatagccg cctgctttac tattgccgct
    28081 ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc
    28141 actggcgcga atcaaacttg cttgtccaca ctgcggtttt ttgttcagcg catgtgcacg
    28201 tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa tctgcgcggt tatattccag
    28261 catcgccagc gccaggttac agcgataatc cagcgggtcc agcgccacgc cgcgcaggta
    28321 gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca
    28381 ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccggcaa
    28441 cggcaactcc tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga
    28501 gagttcaatg gtcagtctgt caggattgat accgtgcaac acgccctgga tggcggtcgc
    28561 tggtgtcagg gccacggcat catcgagcaa cgcgttgcat ttgccgattt cgcggatcgc
    28621 cagacggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgctcact
    28681 acgctggagt tttatcaccg catcgcggga ggcattttgc accatgccca gagaatgata
    28741 aggcaggaaa tactgctcga aacgcttctc ttcgtaagca tcaagccagg taaaatcagg
    28801 ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat tgtcggtcag
    28861 actcttatcc cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg
    28921 cgcaatatgg tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc
    28981 gacaaaatcg tactgcgatt tttcggccat atatgaggtt ggaacaggca catttttata
    29041 gcgagaaatg tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg
    29101 gaaagcggag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac
    29161 gctctgatgc ccttcacccc ctttcactgc cgggttgacc caccacaaga aatgacgcgg
    29221 cgtggcgttg ccgttataga cgcggctggc gatttccagt gccgcccggt cagggcgcag
    29281 ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccga cccacaccgt
    29341 ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg
    29401 atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca gcatccccac
    29461 cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca ctttatccca
    29521 tgcgcgatgc acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc
    29581 cagccacacc gcctgctagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc
    29641 gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg
    29701 tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac
    29761 tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg
    29821 tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta
    29881 gtgaagatga gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt
    29941 ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa
    30001 atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg
    30061 aatattgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg
    30121 gaaggtgact ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct
    30181 aatgcttgtt taagtccgaa aggcgaattt gagtatttac agattgatgt accgacgccg
    30241 caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac
    30301 cgcaccatca atctggcgct ggcaatccac gggcaggttg atccggtgac cggcgtatcg
    30361 caaaccatgc cgcaagcgcc gtggacaacg ccagttgagg tgaagtatct gctggaagag
    30421 aagctgggca ttcgggtgct ggtcgataat gactgcgtga tgctggcgct ggcggagaaa
    30481 tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc
    30541 tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacagatt
    30601 ggtcacacca tcgttaatcc ggatggagtc gtctgcgact gtggacgtta cggcagcctg
    30661 gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa
    30721 ccggttaata ctcagcttga gcctgaaaaa ctgacaaccg cgcagttaat cgctgcctgg
    30781 cagagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg
    30841 agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt
    30901 tgtgcctttg gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc
    30961 gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc
    31021 gcacaacagg tgctgggaat tggctatttg tatgttgagg cgcagttacg acagatttga
    31081 tggcgcgata acgtagaaag gcttcccgaa ggaagccttg atgatcataa acgaaaaatt
    31141 gcctgatgcg ctacgcttat caggcctaca cggagattgc aatatattga atttgcaaga
    31201 ttttgtaggc ctgagaaggt gttcatgccg catccaacat gaacaacgag cacattgtca
    31261 gcaaatcacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc gagaacttta
    31321 cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc acacatccag
    31381 ccagccagtt ctttcgcttc ggcttcttta aagccgcgac gggtaatcgc cggagtacct
    31441 acacgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct gtttttgttg
    31501 acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag gtttttatca
    31561 accagatcaa ccaggaacag gtggttatca gtgccgccgg aaaccacttt gtagccgcgc
    31621 tcgaggaaca cttctaccat cgctttagcg tttttcgcga cctgctgctg gtaagttttg
    31681 aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac gtgcatcaac
    31741 ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag ctcttcgcta
    31801 ccacctttcg ccaagatcag gccgccgcgc ggacccgcca gggttttgtg agtggtggta
    31861 gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac caggcccgca
    31921 acgtgcgcca tatcaacgaa cagataagca ccgatgctgt cagcgatttc acgcattttc
    31981 gcccagtcaa ccacgccgga atatgcagag aaaccaccga taatcatttt cggcttgtgt
    32041 tctttggctt gtttttccag atcggcgtaa tcgatatgac cggtagcatc gataccgtaa
    32101 ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt caggtgaccg
    32161 ccatgcgcca ggttcatacc cagaacggta tcacctagtt ccagcagcgc ggtgtagacc
    32221 gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc gccgaacagt
    32281 tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca accgccgtag
    32341 tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc ctgcgcctgc
    32401 attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc ttcctgacgt
    32461 actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat gttcatttca
    32521 cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc tttataggcg
    32581 gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca ggtcttgaca
    32641 aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa taaagagctt
    32701 gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt catcacattc
    32761 tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata agatgcattt
    32821 gagatacatc aattaagatg caaaaaagga agaccatatg cttgacgctc aaaccatcgc
    32881 tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa ccgcccattt
    32941 ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca tgagtaacca
    33001 gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg ccagtaatat
    33061 tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc acaccagctt
    33121 ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa cgctggacga
    33181 aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg gtgtactggc
    33241 taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca aagccggtgg
    33301 ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg cgcttatcac
    33361 cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc cggggcaata
    33421 tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc agtactcttt
    33481 gactcgcaaa ccggatggca aaggctatcg tatcgcggtg aaacgcgaag agggtgggca
    33541 ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac tggtcgctcc
    33601 ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa tctctaccgg
    33661 tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag gccacacagc
    33721 acaagtgaac tggttccatg cggcagaaaa tggcgaagtt cacgcctttg ctgatgaagt
    33781 taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc gtcagccgag
    33841 cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt tgagcaaact
    33901 ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg ttggcttcat
    33961 gcagtttacc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca ttcattacga
    34021 atgctttggc ccgcataagg tgctgtaatt tgatgttgcc ggatggaaac atccggcaac
    34081 ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc gaatgacccg
    34141 agccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg ccgtgcgaat
    34201 aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa ttttcacttt
    34261 cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc ctttctggcg
    34321 accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg ccagtgcttc
    34381 gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca tgctattcct
    34441 tgaaaaggtt gcctgtcttt tgatctgcta aacgtaacac ataacgccaa ttcattcctt
    34501 gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg tccggttgcg
    34561 ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg atttgcagca gcttacgcaa
    34621 gtagttgagt tcaaaatgat tacgtgcctc aacaaaggtt ggcagcgccg tattttcacc
    34681 ctccagcgcc tgctccacca gcgcatcact aatcaccgga gatgaggtca gcgccacgca
    34741 ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg tcatcaggcg
    34801 tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg ccgcctggcg
    34861 caacaggtga tttgccagta gcggaatgtc ttctgtgcgc tccgccagtg ccggaatttt
    34921 caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc gcgccatcgc
    34981 ttttgacaga tcacggtgag tggcagaaat aatccgcaca ttgatatcaa tatcgcggtt
    35041 actgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga cctgtaacgg
    35101 cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg cctggaataa
    35161 accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa acagctccga
    35221 ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt tgctgttgcg
    35281 cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc tctgaccgtt
    35341 aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca aacgcagcat
    35401 cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg gcgcggattg
    35461 ctccagcgca tcgtcaattg cctgatatag cgcatcttta tcgacaggct tggtgaggaa
    35521 actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat gcgcagtaag
    35581 aataattact ggcattcccg gctgcacttt ctggatttca gcaaatagct gcataccgtc
    35641 catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat tcagtacccg
    35701 taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg tcaggcgcag
    35761 gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg caggtttatg
    35821 gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg tgcggcgtat
    35881 ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata tcggtcaggt
    35941 tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt tgctggcgca
    36001 atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg cgctcttccg
    36061 ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg cgaacctgtg
    36121 ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc tcatacggcg
    36181 taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag ctgccgtcgt
    36241 catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca caatccatcg
    36301 cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc cagatgtcgc
    36361 tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct tctgctggcg
    36421 tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga catggcagcc
    36481 ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata ttcaccagac
    36541 ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga cagcgcggag
    36601 aaaccggggc gtacatatcg tacgtgaaga tttcgagcac tacccggggc cgaactgaca
    36661 aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg acgacggtaa
    36721 ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt ccccttgcat
    36781 acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca ccgccccttt
    36841 tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct cttgcggaat
    36901 gggcgtgcct gtattgatga catcaatata aacccgcgca ccatgtaaac tgctgcgaag
    36961 gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat tatccagtac
    37021 gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga cgtcggtatg
    37081 catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca ccagcggtgc
    37141 taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt aatcaagcag
    37201 ttgttcgatc agtttttgca aattgcagct gctgctatca agaatgctca ccacctcttt
    37261 ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc cctcgcgcat
    37321 actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat ggcgttggga
    37381 ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg agcgtaactc
    37441 gctcggtcca cagaacgaaa cgctattgcc cagagaacgc ccttccccca gacggttgat
    37501 catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta gcaccatcac
    37561 cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac gttcggcgat
    37621 ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct gcaccatttc
    37681 ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg gaccactgtt
    37741 gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt aaagtttatc
    37801 gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc gctggctttg
    37861 ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc tacgctccat
    37921 ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg tagtgcggtt
    37981 aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc atgccagcac
    38041 caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta atgagcgggg
    38101 aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc tgatgctaac
    38161 cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat ggaataaggc
    38221 ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat cagaagttgg
    38281 acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg aaggggctga
    38341 cataagtcgg tgaatgagcc actgcttact attatgcagt aactgtgcca ataaagaaaa
    38401 tagtttggta acgtactgat tatacgttgc tttgagggat ttatggcttc ttcgctattt
    38461 gaatgtaaat cagccactgt gtcgctaaaa agagacaact taagataaac ttattagatt
    38521 gcaataaaat caatcattta agtgtcgcca gaaagcgaca cggtaaacca cccattgtcg
    38581 tgatttacag acacaaaaaa gctcccggag ttgggagctt atgatagtgg ttggtgctta
    38641 atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt accccaactg cttacgcgca
    38701 ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttccggatg ccaggagttg
    38761 ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg accactttca
    38821 gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt tagccgggta ggtttcagtg
    38881 actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc cagtgccgcc
    38941 agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc aatcggcatt
    39001 tgcgagccca ccatcccctg caacagcaga gacgggcttt gggttacttc aaccaggctg
    39061 aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac gtggccacaa ctcactcccc
    39121 gggatcagtt cacgcaggtt agacatcatc tggcaaccgt tacatacccc cagggccagc
    39181 gtttgcggac gatggaagaa ggttgcaaac tcatcgcgta cacggtcatt gaacaggata
    39241 gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc tccgcacgcg
    39301 accagggcgt gaaaatcctc cagtcccgta cgtccagtca gcaggtcgct catatgcacg
    39361 tcgatagcat caaagccccc acgttggaaa gctgccgcca tttcaacatg cgagttcacg
    39421 ccctgctcac gcagtacggc aactttcgga cgtgcgccag tggcaatata cggtgctgcc
    39481 acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc gtcgttagat
    39541 ttcgcctgat gctcctgatc ggcacactcc gggttgtcac gcaggcgctg catctgccag
    39601 gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac agtctgcccg
    39661 ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg gacgcaatcc
    39721 gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc acgaacctga
    39781 atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc cagagaggcg
    39841 atatccgcgt caatgccaca atgacctgca aaggccattt ccgccagcgt taccagcagg
    39901 cctccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag cgcctgaatc
    39961 gcgtcataga agcctttcag ttggacaaca tcgcgtacat ctgccggttt gtcgccaagc
    40021 tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt gcccaaatca
    40081 atcagcagca gtgcgttatc ttcggtacaa agctgcggcg tgatggtgtg acgtacatct
    40141 tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc gcgctcttcg
    40201 ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg gatcgtcagg
    40261 cccagcgccg gacaaagttc ttcacccacg gctttaacgg cttcatacag gcccgcatct
    40321 tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg tttgatatcg
    40381 ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg ggcagaggcg
    40441 gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc accgtagtag
    40501 ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca cggccccacc
    40561 atctgatcgc gcgccaccat gccggttacg ctgcggtcac cgatggtcac caggaaagtt
    40621 ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat ggtgatccct
    40681 tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt catcttcggc
    40741 gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa atgacgatcg
    40801 tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg tgcgcgctca
    40861 cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag cacgtagcgt
    40921 tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc gcttagaata
    40981 tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca ccagctccgg catcgcgtta
    41041 gaaagaccgc ccgcaccgac gtcgtggata aacagaattg ggttagcatc accaagctgc
    41101 cagcaacggt cgatcacttc ctggcaacga cgctccatct ccgggttgtc gcgctgtacg
    41161 gaagcaaagt cgaggtcggc atcagactga ccagacgcca tggaagacgc cgcgccgccg
    41221 ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc gacgttgatc
    41281 tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag catgatcggt
    41341 ttgtgataac cgcgcagctc ttcgccattg tggctgttca ctttttcttc ataagtacgg
    41401 aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa acgccgcacc gcccagcggg
    41461 ccttcggtca tgatgtccag cgcggtaaca atgcgctcag gcttaccgaa atcttcttcc
    41521 cacggctgtt cgaagccagg aattcgcagg ttggatacgg agaaaccaac caaaccagct
    41581 ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc ggaaccggtc
    41641 gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac tttcatcagg
    41701 atgtgcgccg gttcctgatg gaaattgtag cggcccgttt cgtggtcagc aaagtagcgg
    41761 cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag aacgtgatct
    41821 ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg ttgttcaccg
    41881 tcgataatcc agtcggcgtt aaaaattttg tggcggcagt gctcagagtt cgcctgagcg
    41941 aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc atcctgcaga
    42001 tagtcaattt catcttccgc cagagccaag ccaagacgca ggttagcgtc gatcagcgcc
    42061 tgacggccct gccccagcaa atcaacgctt gtgaccggag tcggttgatg gtgggcaaac
    42121 aactgctctg catcatctaa atcaaaaaag accgtttcca tcatgcggtc gtgcagttca
    42181 gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata gtaagcaacg
    42241 ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc ggtcgctttc
    42301 gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt gccttgcggg
    42361 gcgtggctgg cgagtgccgg accatatttc agcaggcgtt caagttgtgc gtgctcatca
    42421 tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat attgtgaacc
    42481 gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc cgacagtgca
    42541 ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc ggtgtaccca
    42601 agggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg
    42661 gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc
    42721 atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc actattatta
    42781 cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa aaattaaaga
    42841 ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc tggccatcca
    42901 ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc
    42961 gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa ccttttggcc
    43021 tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg aaagtgaccg
    43081 tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc gacctgctgg
    43141 cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc cctacctatt
    43201 attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt acgctgggca
    43261 acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtagttaac gatctccaga
    43321 ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa aaaggctctg
    43381 cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct gattctgtcg
    43441 ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat atcaccgatg
    43501 aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc gccgccctgc
    43561 tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa gagaaatacc
    43621 tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc gccgtcgatg
    43681 cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt gactggcgtt
    43741 tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact tcaccgacgg
    43801 gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc attacggatc
    43861 gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat atgatgagta
    43921 aagtgccgga aagtgtgccg gagaacgaac ggatctggtt tgccctcgct gcgtacaata
    43981 tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg
    44041 acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc tattacagca
    44101 agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa aatattcgta
    44161 agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct acagaagcgg
    44221 cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc aaagagaaat
    44281 ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat tctccctctc
    44341 tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag gattgcgctt
    44401 ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc aacgccgcgc
    44461 actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc ggatgatgca
    44521 gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca aagaccacgc
    44581 gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca agcgtgacat
    44641 acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc cgcagggcca
    44701 tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag ccttcgccga
    44761 ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca tcccaggcac
    44821 gtttcgccag cgtcagcgcg tgacgcatcc aatattcgtg gctaaattcg acttcagaca
    44881 aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat gctttactct
    44941 attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga cagaaataaa
    45001 gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc ggcgtgccga
    45061 ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc aatacccaac
    45121 caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc cagggcgtat
    45181 cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag atatcagcat
    45241 cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg gtaacattgt
    45301 cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg ctgtgaccaa
    45361 aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct tttatcaata
    45421 acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc ggttggcgac
    45481 gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt
    45541 caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc
    45601 ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc cggcaacaat
    45661 cgaccgcatc atggctaact ggaattttta attcatggca attagcggca atggaatata
    45721 aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc gcgaggaaag
    45781 atatgaacgg cttacttcgt atccgtcagc gttaccaagg gcttgcccag agtgataaaa
    45841 aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc tctcagcaac
    45901 tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct
    45961 ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc cagccggaat
    46021 cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg
    46081 aaaaactgat taaagaaaat accgccgcta tgtacgcaac gctaaacgtt aatagtgaag
    46141 agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt ctgaccggta
    46201 ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag attggcttca
    46261 atgctgccgc actgcgcgat atgcatgcgc tactcgcaac agtacaggcg tcgtcccctg
    46321 acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac ctggcggcag
    46381 atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact ccgaatgccc
    46441 tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg acaaacagtg
    46501 cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc attgcgctga
    46561 ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg ctggtgaaaa
    46621 aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt
    46681 ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg tgaaatcaaa
    46741 taaaaacaca tttattttca tagtgataca gcaatgtgtt attgaatgtg ttgttggatt
    46801 tatggtgcaa aaaaattttg cataagttca gcaacacaaa gaacaccact ttctttaacg
    46861 tcctccgcgc cctaaaggac ggggctttac ggcgcaaaag gcctgtgaca tacaacatta
    46921 acttttcagt caaccgtagc acgttcttgt atacgccgtg tctgcggttt tatataaccc
    46981 tggctggaaa cctcttatac aaagtcgatt cgccaacatc atagatgatc gccaccttct
    47041 ggcgagaaac tcctgatgca attagtcgcc cggcctgcgc ccattattct ggtgaaagtt
    47101 tgggacggcg accgccaatt cgtccctgtg cgcgagcagc ttccagtcct gcttttgttc
    47161 gttcaacaat cagttcacgt tccatttcag ccagggcacc catcacatgg aagaaaaagc
    47221 gtcccattgg tgtgctggta tcaattgaat cagtcagact acgaaagttg atgcctcgtt
    47281 cgcgcaacgc ctccaccagc acgacaagat gccgcatact gcgccccagc cgatccagct
    47341 tccagacaac cagtgtgtcg cctgccgata atgtcttgag cagttttttc agccccggtc
    47401 tttcggactt tgtaccactt atcttgtctt caaaaatcag ctcgcatcct gcacagttca
    47461 acgcattacg ttgtagatcg gtattctggt catttgttga tacg
//
LOCUS       DAJTRW010000024        45507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRW010000024 DAJTRW010000000
VERSION     DAJTRW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            114..2267
                     /locus_tag="OO326_001440"
     CDS             114..2267
                     /locus_tag="OO326_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5965872.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO326_001441"
     CDS             2320..4050
                     /locus_tag="OO326_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965873.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO326_001442"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO326_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5965874.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO326_001443"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO326_001443"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5965875.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO326_001444"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO326_001444"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5965876.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO326_001445"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO326_001445"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5965877.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO326_001446"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO326_001446"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5965878.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO326_001447"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO326_001447"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5965879.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO326_001448"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO326_001448"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5965880.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO326_001449"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO326_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5965881.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO326_001450"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO326_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5965882.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO326_001451"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO326_001451"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5965883.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO326_001452"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO326_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5965884.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO326_001453"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO326_001453"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5965885.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO326_001454"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO326_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5965886.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO326_001455"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO326_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5965887.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO326_001456"
     CDS             16308..16847
                     /locus_tag="OO326_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5965888.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO326_001457"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO326_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5965889.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO326_001458"
     CDS             complement(18206..18553)
                     /locus_tag="OO326_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5965890.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO326_001459"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO326_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5965891.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO326_001460"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO326_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5965892.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO326_001461"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO326_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5965893.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO326_001462"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO326_001462"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5965894.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO326_001463"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO326_001463"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5965895.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO326_001464"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO326_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO326_001465"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO326_001465"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5965896.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO326_001466"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO326_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5965897.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO326_001467"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO326_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5965898.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPICKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO326_001468"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO326_001468"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5965899.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO326_001469"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO326_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5965900.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO326_001470"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO326_001470"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5965901.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO326_001471"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO326_001471"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5965902.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO326_001472"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO326_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5965903.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO326_001473"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO326_001473"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5965904.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO326_001474"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO326_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5965905.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO326_001475"
     CDS             complement(40011..40466)
                     /locus_tag="OO326_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965906.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO326_001476"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO326_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO326_001477"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO326_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5965907.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO326_001478"
     CDS             complement(41309..41986)
                     /locus_tag="OO326_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5965908.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO326_001479"
     CDS             complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO326_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5965909.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO326_001480"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO326_001480"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5965910.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO326_001481"
     CDS             complement(43192..43650)
                     /locus_tag="OO326_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5965911.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO326_001482"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO326_001482"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5965912.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..>45507)
                     /gene="adrA"
                     /locus_tag="OO326_001483"
                     /gene_synonym="dgcC"
     CDS             complement(44596..>45507)
                     /gene="adrA"
                     /locus_tag="OO326_001483"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5965913.1"
                     /translation="WWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMW
                     VGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSA
                     PLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETML
                     RNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIG
                     RFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSH
                     YREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11831 a        11872 c        11125 g        10679 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atccactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgagcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttacagat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtt gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagacct ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg tgcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactct ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacta gccacca
//
LOCUS       DAJTRW010000025        42781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRW010000025 DAJTRW010000000
VERSION     DAJTRW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(90..626)
                     /gene="ssb1"
                     /locus_tag="OO326_001484"
     CDS             complement(90..626)
                     /gene="ssb1"
                     /locus_tag="OO326_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5965914.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            880..3702
                     /gene="uvrA"
                     /locus_tag="OO326_001485"
     CDS             880..3702
                     /gene="uvrA"
                     /locus_tag="OO326_001485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5965915.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3737..4093)
                     /locus_tag="OO326_001486"
     CDS             complement(3737..4093)
                     /locus_tag="OO326_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5965916.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4097..4513)
                     /locus_tag="OO326_001487"
     CDS             complement(4097..4513)
                     /locus_tag="OO326_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5965917.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4624..5337)
                     /gene="aphA"
                     /locus_tag="OO326_001488"
     CDS             complement(4624..5337)
                     /gene="aphA"
                     /locus_tag="OO326_001488"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5965918.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5739..5943
                     /locus_tag="OO326_001489"
                     /pseudo
     CDS             5739..5943
                     /locus_tag="OO326_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="OO326_001490"
     CDS             complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="OO326_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5965919.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7892..8971)
                     /gene="alr"
                     /locus_tag="OO326_001491"
     CDS             complement(7892..8971)
                     /gene="alr"
                     /locus_tag="OO326_001491"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5965920.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9024..10439)
                     /gene="dnaB"
                     /locus_tag="OO326_001492"
     CDS             complement(9024..10439)
                     /gene="dnaB"
                     /locus_tag="OO326_001492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5965921.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10522..11505
                     /locus_tag="OO326_001493"
     CDS             10522..11505
                     /locus_tag="OO326_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5965922.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="OO326_001494"
     CDS             complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="OO326_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5965923.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="OO326_001495"
     CDS             complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="OO326_001495"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5965924.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13447..14432)
                     /locus_tag="OO326_001496"
                     /pseudo
     CDS             complement(13447..14432)
                     /locus_tag="OO326_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14750..15265
                     /gene="zur"
                     /locus_tag="OO326_001497"
     CDS             14750..15265
                     /gene="zur"
                     /locus_tag="OO326_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5965925.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15307..15516)
                     /locus_tag="OO326_001498"
     CDS             complement(15307..15516)
                     /locus_tag="OO326_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5965926.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15632..16957)
                     /gene="dinF"
                     /locus_tag="OO326_001499"
     CDS             complement(15632..16957)
                     /gene="dinF"
                     /locus_tag="OO326_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5965927.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="OO326_001500"
     CDS             complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="OO326_001500"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5965928.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17748..18116)
                     /gene="dgkA"
                     /locus_tag="OO326_001501"
     CDS             complement(17748..18116)
                     /gene="dgkA"
                     /locus_tag="OO326_001501"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5965929.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18287..20710
                     /gene="plsB"
                     /locus_tag="OO326_001502"
     CDS             18287..20710
                     /gene="plsB"
                     /locus_tag="OO326_001502"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5965930.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="OO326_001503"
     CDS             complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="OO326_001503"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5965931.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="OO326_001504"
     CDS             complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="OO326_001504"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5965932.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22470..24050)
                     /locus_tag="OO326_001505"
                     /pseudo
     CDS             complement(22470..24050)
                     /locus_tag="OO326_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24279..25199)
                     /gene="malM"
                     /locus_tag="OO326_001506"
     CDS             complement(24279..25199)
                     /gene="malM"
                     /locus_tag="OO326_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5965933.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="OO326_001507"
     CDS             complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="OO326_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5965934.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26763..27878)
                     /gene="malK"
                     /locus_tag="OO326_001508"
     CDS             complement(26763..27878)
                     /gene="malK"
                     /locus_tag="OO326_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5965935.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28243..29433
                     /gene="malE"
                     /locus_tag="OO326_001509"
     CDS             28243..29433
                     /gene="malE"
                     /locus_tag="OO326_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5965936.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29587..31131
                     /gene="malF"
                     /locus_tag="OO326_001510"
     CDS             29587..31131
                     /gene="malF"
                     /locus_tag="OO326_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5965937.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31146..32036
                     /gene="malG"
                     /locus_tag="OO326_001511"
     CDS             31146..32036
                     /gene="malG"
                     /locus_tag="OO326_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5965938.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32407..33882
                     /gene="xylE"
                     /locus_tag="OO326_001512"
     CDS             32407..33882
                     /gene="xylE"
                     /locus_tag="OO326_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5965939.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="OO326_001513"
     CDS             complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="OO326_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5965940.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34462..34605)
                     /locus_tag="OO326_001514"
     CDS             complement(34462..34605)
                     /locus_tag="OO326_001514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965941.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34606..34884
                     /locus_tag="OO326_001515"
     CDS             34606..34884
                     /locus_tag="OO326_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965942.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34931..37027)
                     /locus_tag="OO326_001516"
     CDS             complement(34931..37027)
                     /locus_tag="OO326_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5965943.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37027..37764)
                     /locus_tag="OO326_001517"
     CDS             complement(37027..37764)
                     /locus_tag="OO326_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5965944.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37761..38399)
                     /locus_tag="OO326_001518"
     CDS             complement(37761..38399)
                     /locus_tag="OO326_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5965945.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38513..38755)
                     /locus_tag="OO326_001519"
     CDS             complement(38513..38755)
                     /locus_tag="OO326_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965946.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39218..40867)
                     /gene="pgi"
                     /locus_tag="OO326_001520"
     CDS             complement(39218..40867)
                     /gene="pgi"
                     /locus_tag="OO326_001520"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5965947.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41392..42741
                     /gene="lysC"
                     /locus_tag="OO326_001521"
     CDS             41392..42741
                     /gene="lysC"
                     /locus_tag="OO326_001521"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5965948.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10643 a        11013 c        10503 g        10622 t
ORIGIN      
        1 gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa
       61 acaatataac ctattgtttt aatgacaaat cagaacggaa tgtcatcatc aaagtccatc
      121 ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg
      181 ctgaactgat tgccaccctg cggctgctga ggctgacccc aaccgccctg cggctgacca
      241 ccaccgatat tgccacctgc cggagcgcca ccaccctgac gaccacccag catctgcatg
      301 gtgccgccaa cgttcaccac gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc
      361 catttacggg tacgcagctg accttcgata taaacctgag aacctttacg cagatattcg
      421 ctcgccactt ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgttctttc
      481 atctcgccgg tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaact
      541 gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata
      601 accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa
      661 gtgtaaacgc gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc
      721 cgggaaacaa acctggccag acattgttac acaacactcc ggataatgca ttccaatact
      781 gtatattcat tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca
      841 tgccaccggg caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg
      901 ggcgcccgca cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt
      961 gtcgtgaccg ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc
     1021 gaagggcagc gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg
     1081 atggaaaagc cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag
     1141 aaatcgacgt ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat
     1201 ttgcgtttgt tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg
     1261 gcggcgcaaa ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt
     1321 ctgatgctgc tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag
     1381 aacctggcaa gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc
     1441 gatccgccga aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc
     1501 aaggtgcgtg acgatcttac ccaacgtctt gcggagtcgt ttgaaaccgc gctggaactt
     1561 tccggtggta cagcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc
     1621 tccgccaact tcgcctgccc aatttgcggc tacagtatgc gtgaactgga gccgcgactg
     1681 ttttcgttta acaacccggc aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa
     1741 tatttcgatc ctgaccgcgt gatccaaaac cccgagctgt cactggctgg cggtgcgatc
     1801 cgtggctggg atcgccgcaa cttctattac ttccagatgc tgaaatcgct ggcagatcac
     1861 tataagttcg acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg
     1921 ttgtacggtt ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc
     1981 tccatccgtc gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag
     2041 acagaatcca gtgcggtacg tgaagaatta gccaagttta tcagcaatcg cccatgcgcc
     2101 agctgcgaag ggacgcgtct gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg
     2161 ctgcctgcta tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa
     2221 ctcgctggtc agcgggcgaa gattgcggaa aaaattctta aagagatcgg cgatcgcctg
     2281 aaattcctcg taaacgtcgg cctgaattac ctgacacttt cccgctcggc agaaacactt
     2341 tccggcggtg aagcccagcg tatccgtctg gcgagccaga ttggtgcggg cctggttggc
     2401 gttatgtacg tgctggacga gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg
     2461 ttgggtacgc ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcatgac
     2521 gaagacgcaa ttcgcgccgc tgaccatgtg atcgatatcg gtcctggtgc gggtgttcac
     2581 ggcggtgaag tggtcgcaga aggtccgctg gaagcgatta tggcggtgcc tgaatcgttg
     2641 accgggcagt acatgagcgg taaacgcaag attgaagtgc cgaagaaacg cgttccggcg
     2701 aatccagaaa aagtgctgaa gctgacaggc gcacgcggca acaacctgaa ggacgtgacg
     2761 ctcacgctgc cagtcggtct gtttacctgc atcacagggg tttcaggttc cggtaaatcg
     2821 acgctgatta acgacacact gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc
     2881 gccgaaccgg caccgtatcg cgatattcag ggactggagc atttcgataa agtgatcgat
     2941 atcgaccaaa gcccaattgg tcgtactcca cgttctaacc cggcgaccta taccggcgtg
     3001 tttacgcctg tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg
     3061 ccaggacgtt tcagctttaa cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt
     3121 gtgatcaaag tggagatgca cttcctgccg gatatctacg tgccgtgcga tcagtgcaaa
     3181 ggtaaacgct ataaccgtga aacgctggaa attaagtaca aaggcaaaac catccacgaa
     3241 gtgctggata tgaccatcga agaggcgcgt gagttctttg atgcggtgcc agctctggcg
     3301 cgtaagctgc aaacgttgat ggacgttggt ctgacgtaca ttcgcctggg gcagtccgca
     3361 accacacttt ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc
     3421 ggcaccgggc agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat
     3481 attcagcaac tcctcgacgt gctgcataaa ctgcgcgatc agggcaatac cattgtggtg
     3541 attgagcaca atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa
     3601 ggcggcagtg gcggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc
     3661 gaagcttcgc atacggcacg cttcctcaag ccgatgctgt aatcgttaag gccgctttct
     3721 gagcggcctt ttcctttcag agttgtacca gcaatttacg cttttcttcc ggtagtaaat
     3781 tcactgcctg ctgataagac gcatccacca gataatagat ttgcgaatcc ggcagcgaac
     3841 cgtcgagata cacggtgctc cagtgcgctt tattcagatg gcggctagga cgcacatcgc
     3901 tgtgctgctg acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc
     3961 gattttctac ttctttcacc atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt
     4021 tccagtcgtt atgcacgctc tgttctgcgc ttggttttgc catgcaatat tgtagcaact
     4081 ccgaaatggt cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt
     4141 ggatgcgatg ttcccccagc cagatgcctt gccaagtgcc ggtctgaata cgccctttat
     4201 gtaccggcaa tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt
     4261 ctgctccctc atagtcatgc tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac
     4321 actccatgtc gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg
     4381 tatgttgcag caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat
     4441 tcagaatttc atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct
     4501 tttgatacca catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag
     4561 cgaaaggata aaggtgtgat taaaaaaaca gcattcggga gagcatcact ctctcccgct
     4621 ctgtcagtat tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg
     4681 tttgtaggta gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc
     4741 ggcggtaata tcgttatcgg aatcgccata aaaaattcgg atatttttat cctgcagcca
     4801 ttgcgatttt gtattttgcc ctggtttatc gcccgcaaag attaccggat tcatgttggt
     4861 ggcaggaata tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct
     4921 acgaccagtc acaaagaaga tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg
     4981 agcgacctct tttggaatgc tgaattcatc ccagccatta ttcatttttt cccagaacac
     5041 aggatttttc agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaaccc
     5101 cggactggaa aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc
     5161 tgcgaggcta ttttcaattt gtgcgaccga aacccaatga attggtgcct gttcagcaag
     5221 cctggcaacg ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga
     5281 actgtttaga gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt
     5341 tttccctaaa tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc
     5401 tgaccataac gccagctgcc ggggaaagga aggattatct gcggtttttg tgatgaaaag
     5461 caggatgatt atatttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt
     5521 tattactcga tgtaaggata cattaatttt acgtttatta tgatgaatcc tcggcaaatg
     5581 agcgaaattt gatagaacat ttatctgctg tacgatttaa tgaacttaat gatacagcat
     5641 tacaaaaaca atcgaagttt ataaagatga tttctgattg accagccccc taccctacaa
     5701 tggtgattaa ttttattact cgcgatggag atgatgatat gaatatatca tatgtgaata
     5761 gcaacaaaac gacatcttta cctgttgagc tagatgcact aaataataaa gatatctctt
     5821 atgccaaaga tttcttttta tacatagaaa cacagttaaa aattgcaaaa gatttttgta
     5881 gacctggaga agaagtatca agttctattg caagtaaagt ttttcacgca tttattgatt
     5941 tagttaacaa aatcaggggt aagaaagact ttatgtatat ttgcacgctt tgctgttttg
     6001 ccgaggaggt taaaggtgat tattctcatt acaggacctt tttatttgat attggtaatc
     6061 aatataaggt taaacttaca caaagcggaa aaaagagttc tctttaacat tagaatttaa
     6121 cgatactata attgaatctc agaaagtcac aggcaataag gcaaagcata ttcttgagga
     6181 tatagaaata ttctatcgta acaaacccga tacttattat taaatttaat aatggttaga
     6241 tgtgcagaaa ttattactcc acacgaaaaa atagggaaag gaattccaga taattgggat
     6301 aaacttaaca ataaaagaac tgacaacgtt ttattatctc tcacgtagaa caacggcatc
     6361 aaaaaaatca tctacaaaga aatagtgaaa gcgacgacag tttaatttca ctgcaggctg
     6421 ggtaactcca gcctgctttc ctgcattaca tcaccgcagc aaacgccttt gccacacgtt
     6481 gtacatttgc cgtatttaac ccggcgacac acatgcgacc gctggcgatg agatagacac
     6541 caaattcttc acgtagtcgg tcaacctgag cggcacttaa accggtataa ctgaacatgc
     6601 cgcgctgatt aagcagataa tcgaaattgc gttctggcat ctctgtgctt aataccttca
     6661 ccaattcctg acgcattgcc agaatgcgag tacgcatctc ttctacttcc gccagccagc
     6721 tggctttcaa tgcctcgtca ttcagcactg cagccaccac ctgcgcacca aaattcggcg
     6781 ggctggagta gttgcggcga actgttgctt tcaattgccc cagtacgcgg ccagcggctt
     6841 cggcatcttc acacagaaca gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt
     6901 tcgagaacga attgctcacc agagcgggta atccagcact ggcaatggcg cgaatagcgt
     6961 aggcatcctc ttccataccg gcactaaatc cttgataggc aatatcgagg aatgggataa
     7021 gctcgcgggc tttgagaatt tcaatcaccg catcccactg atcattagtg agatcggcac
     7081 ccgttgggtt gtggcaacat ggatgcagca acacaatact gcgggcaggt aatgttttca
     7141 gcgtcgccaa caggtcatta aagcgcacgc tgttagtcac ttcgtcatac caggggtaag
     7201 tactcacttc gaatccagcc ccggcgaata ttgctacgtg gttttcccaa gtaggatcgc
     7261 tgacccagac gcctgattcc gggaagtagc gtttcaggaa atccgcgccc actttcaatg
     7321 cccctgagcc gccaagggtt tgaatggttg ctacgcgctg ttgttgcagt accggatggt
     7381 ccgcaccaaa cagcagcggc gcaatggcat ggcgatagct gttaagccct tccatcggta
     7441 aataaagcga agcgccatgg ggctgcgcat tcaggcgcgc ttccgcctcc gccacggctt
     7501 tcaattgtgg aataattccg tcttcgttgt agtacagacc gatacttaaa ttcactttgt
     7561 cgctgcgagg gtcttcttta aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg
     7621 cgtcaacttt ttgaaacacg cgatggttct ccaggtttac gggcaggtgg ttaaaacaca
     7681 ataaaccgga agaaggcgaa gatcgagtgg atgttcagga gcgaacggca attagcaaca
     7741 gagtgagact aatgataaac gtacatccgc cagaggcacg acctttataa gagcatgaaa
     7801 gaacatcaac ttattgaatt tttaggattt tattggccgg ataaggcatt cacgccgcat
     7861 ccggcacaga caatcaaata ttacagaacg attaatccac gtatttcatc gcgacccttg
     7921 aagtcaggcg cgtaataagt tcgtaagcgc ttactttcgt catttcagcg atacgttcta
     7981 cgggcaaacc ttcgccccat aaaatgaccg gatccccggc tttgtcctgc gcctgtggac
     8041 ctaagtctac gcagatcata tccatcgcca cgcgcccgac aatcggtact tcgcgaccgt
     8101 tcaccagcac tggcgtaccg gacggcgcgg cgcgcggata accatcgcca tagcccatcg
     8161 cgactacgcc aagacgggta tcacgttcgc ttacccaggt tccaccataa ccaacaggct
     8221 ctccggcttt atgctcacgc acggcaatca ggctggaggt tagtgacatc actggctgac
     8281 agccaaaatc ggcaccggtg gagcgatctt ccagcggcga gacgccataa agaatgatgc
     8341 ccgggcgcac ccagtcaaaa tgcgactgtg gccacagcag aatgccaccc gacgcggcaa
     8401 tggaacgttg accaggtttg ccttcgcaaa aggtattaaa gatagcgagt tgtttctcgg
     8461 ttgcgccaca ttttggttca tccgcgcgcg caaaatggct gacgatattc accggctgac
     8521 gaacgttttt gcactgggtc aggcgatgat aaaacgcctc agcctgttcc ggccttacgc
     8581 ccagacggtg cataccggta tcgagtttca tccagacggt aaccggctcg tccaagctag
     8641 cctcttccag cgcagccagc tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag
     8701 aaatcgtcgg cagatctctg gcatcaaaaa agccttcgag taacagtaca ggtttggtga
     8761 ttccccccgc acgcagtcgc agagcttctt cgagacgggc tacgccaaag gcgtcagcat
     8821 cggggagcgt tcgcgcggtc tcaagaagac cgtgaccata agcgttcgct ttcaccaccg
     8881 caaccatttt actggcaggt gccagttcac gaagacgttg caggttgtgt cgcagagcgc
     8941 ggcggttaat cacaacagtt gccgcttgca tttgtgttcc ttgataattg tttgctttaa
     9001 ttacctaatt cataaaataa ttattattcg tcgtcgtact gcggccccgc atagttgtcg
     9061 aagcgcgacc attgaccgtt aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt
     9121 ttaccgataa taatttccgc gatgcctttt aaatcactgt tttcgtgata cacctcatca
     9181 cgatagataa acatgatcaa gtccgcatcc tgctcgatag agccagattc acgcaggtcg
     9241 gagttgaccg gacgtttgtc ggcacgttgt tccagagaac ggttcaactg ggacagcgcc
     9301 accaccggca cgttcagttc tttcgccagt gctttcagcg agcgggagat ttctgcaatt
     9361 tccagcgtac ggttatcgga aagcgccggt acgcgcatca gttgcaggta gtcgatcatg
     9421 ataagcccga tgccgccgtg ttcacgggca atacggcgtg cgcgggaacg cacttccgtt
     9481 ggcgtcaggc cggaagagtc atcgatatag atattgcgtt tttcgagcaa aatacccatg
     9541 gtgccggaaa tgcgcgccca gtcttcatca tcgagctgcc cggtacggat tttagtctgg
     9601 tcaacgcgcg acagcgacgc cagagaacgc atcatgatct gttctgatgg catctccagc
     9661 gagaagataa gtactggttt atcctgcaac attgccgcgt tttcgacgag gttcatcgca
     9721 aatgttgttt tacccatcga cggacgcgcg gcgacgatga tcaaatccga cggctgcaag
     9781 ccagcggttt ttttgttgag atcgtcataa ccggtgttta ccccggtaac gccatcgtgt
     9841 ggctgctgaa acaactgctc aatacgcgcc acggttgcgt cgagcacatc ggcgatgttc
     9901 ttcggccctt cgtctttgtt tgcacgactt tcggcaattt taaagacgcg ggattcagca
     9961 aggtccagca gatcttcgct ggtacgcccc tgcggatcaa aaccggcttc agcaatctca
    10021 ttcgcaaccg agatcatctc acggacaacg gcacgttcac gcacgatgtc cgcataagca
    10081 ctgatgttcg ccgcacttgg cgtatttttt gacagctctg ccagataagc aaaaccaccg
    10141 acgctatcga gttgcccctg gcgttccaac gattccgcaa gggtaatcag atcgatagga
    10201 ctaccgcttt cctgcaaacg cgccatttca gtaaagatat gacggtgtgg gcgggtgtaa
    10261 aaatcgtctg ctaccacacg ctcggctaca tcatcccagc gttcgttatc cagcattaaa
    10321 ccgcccaaca ccgactgctc cgcttcgatc gagtgcggag gcactttcag cccggcaact
    10381 tgtggatcgc gttcgcgggg ttcagcctgc tgtttgttga agggtttatt tcctgccata
    10441 gtgaatggag ttaccgaaat aaagaatggg tcgaaacttt accatatgaa gcagaccctg
    10501 acgatacgtt ctggaggaca catggcaaca cgaattgaat ttcacaagca cggtggcccg
    10561 gaagtacttc aagccgtaga gttcactcct gccgatccgg cggagaatga aatccaggtc
    10621 gaaaataaag ccatcggcat caattttatc gacacgtata tccgcagcgg cctttacccg
    10681 ccgccatcgc tacccagcgg attaggtacc gaagcggcag gcatcgtgag taaagtcggc
    10741 agtggtgtaa agcatattaa ggctggcgat catgtagtct atgcgcagtc ggcgttaggc
    10801 gcttacagct ctgtgcataa cattaatgcg gataaagcgg cgattctgcc tgcggcaatt
    10861 tcttttgagc aagctgcggc atccttcctg aaaggcttaa cggtttatta tctgctgcgc
    10921 aaaacctatg aaattaaacc cgatgaacag ttcctgttcc acgcagcggc tggcggcatt
    10981 ggcttaattg cctgccagtg ggcaaaagcc ctgggcgcga aactcatcgg caccgtagga
    11041 accgcgcaaa aagcgcagag cgcgctaaaa gccggcgcgt ggcaggttat taactatcgt
    11101 gaagagaatc tggtcgagcg attgaaagag atcaccggcg gtaagaaagt gcgcgtagtg
    11161 tacgattccg tgggcagaga cacctgggaa cggtcgctgg attgcctgca acgccgcggc
    11221 ttaatggtca gttttggcaa ctcatcaggt gcggttaccg gtgtgaactt aggcattctc
    11281 aatcaaaaag gctcgttgta tgtgacacgc ccttccctgc aaggctatat caccacgcgg
    11341 gaggaattaa ccgaggccag taatgaactg ttctctttga ttgccagcgg tgtgattaag
    11401 gtcgatgtcg ccgagcagca gaaatatccg ctgaaggatg cgcagcgtgc gcatgagatt
    11461 ctggaaagcc gggcgacgca aggttccagc ctgctgattc cataaaagaa atagggcttc
    11521 cacctgggaa gccctttctt tttatagttc ggctgtatgt agggtacagc acgatgaatc
    11581 tgttagaggc gcaatagtga cagatttgat tatcaattcc tattttgttc taaggataaa
    11641 accttaggtt gtgatcatcc gcacaatccc ttagtaacgc cagcggtcat aacgctgata
    11701 tttcggcatt tttggtgctt taatcgcctt aataacccac accaccgcaa tcgccagtag
    11761 taaccacggc agcaacttaa tcatcaatgc cagcataccg ccgaggaaca taatggccgt
    11821 cgccacaacc agcgcggcga taatgcccag caacgaaacg ccggtgacca tcagcatgac
    11881 aaaaaagcca atcacaaaaa gtagttccag catgatgctc tcccaaatat gaaatctctt
    11941 gctggcatta caagaatcat gccaaaaata atctattgat ttaacagcaa aacgccccgc
    12001 gacggtgcgc agggcgtggt gaatttgact actttttggt gaaaagttaa cgcttatccg
    12061 ccaccagttt gagcgcgtgt tccagcacgt taatgtcagc acccgcttta tgggcatttt
    12121 cacttaaata acgccgccac tgccgcgcgc caggaatacc ctggaacaaa cccagcatat
    12181 gccggataat atggccgaga tacgtcccct ggctgagttc atgctcaatg tacggataca
    12241 tggcacgcac tactgccacc ggatcggcat cggtatccga ggaaccaaag atctcccggt
    12301 ctaccgccgc cagaataccc ggattctgat acgcctcgcg cccgaccatc acgccatcca
    12361 tatgttgcaa atgcgcttta gcttcttcca gcgacttgat accaccgtta atcgacattg
    12421 tcagatgcgg gaagtcacgc ttcagttgat acacacgcgg ataatcgagc ggcgggattt
    12481 cacggttttc tttcgggctt aaccccgaaa gccaggcttt acgtgcgtgg atgatgaaca
    12541 tctcacactc gcctttgccg gaaacggtat tgatgaaatc gcagagaaat tcatagctgt
    12601 cctggtcgtc gatgccaata cgcgttttca ccgtcaccgg aatcgacacc acatcgcgca
    12661 tcgctttcac gcagtcggca accagctgcg cattacccat cagacacgca ccaaacatgc
    12721 cgttctgcac ccggtcagac gggcagccga cattcaggtt gatctcatca tatccgcgcg
    12781 cttctgccag ctttgcacac tgcgccagcg ccgccggatc gctaccgccc agttgcaacg
    12841 ctaccggatg ttcttcttca ctgtacgcca gataatcacc tttaccgtga attatcgccc
    12901 ctgtggtcac catttcggta taaagcaacg tattgcggga aagcagacgc aagaaatagc
    12961 ggcaatgtct gtccgtccag tcgagcattg gtgcaacact aaaccgacca ctccagtgaa
    13021 cgtcagtttt ttcaggcatt gcgctggttt ggttgatttt ttgcatttca gaattaccgt
    13081 gcattttcaa atgtagagat tattttatcg atatatcatg gggttatgtt attcagcttc
    13141 actgttcagg aggctcaata gcggggtact ataccataac aacaggaagc gcctgtctca
    13201 ttgcaaaaga aaattgagat cctctcaagg catgaagctc tcacgaagtg atggaaataa
    13261 tcttattagc cgttaacttt gttaaggcca atgataaaca atccaggttc gacgataaat
    13321 aaaaaatcac acattaaact ctggtgatat atctccctgc taatagcatt gatagagaaa
    13381 aaagaaccca ataaatattg ggtcctttta tataatgcct tccatactat cgaagaactt
    13441 cacatattat ttctccgatt taaccccgaa caagtcataa attaatttag aagtgtctgt
    13501 aagtatttta atctcttcct ttgaggttgg gtcaaacgac ttcgcgaagc taattaattg
    13561 tggtgcagca tctcgcattt tgcttaaaat atcaggatcg agcgttcctt cattcaccag
    13621 atgtgacatc ttatccagat agccatcaaa attcatttct cccccatctg ccagacgctt
    13681 catctctccc aggtacttct tcatatcacg ttgatataat ttttcaaact gagctttcag
    13741 gtagctctca ttattttcat taatatacac tgtttctgat aaatctgcgt aggcactaga
    13801 atatgaaagt gaaattaaca gcgaagcaaa taattttaag ctgttgtcat catgttcctg
    13861 gcaggcatta acaaacgtta aaaaaccaga acctattgct tgaaaatgta tattcgctaa
    13921 aggttcatta tccttagatt cctcataaaa tagctgaacc gtggagggaa ggccattctt
    13981 agtttctaca tcaaaagtac attgttcaat gggcattgca tctttattat ccccaggatt
    14041 acatatacta ttaatcgcga aatgaaaaat agcccggtca acaactgatg caataaagtc
    14101 acgatcgttt aaatcctcat cagtgcaatc gtcagtatac ttattgaccc acattttgag
    14161 atcatttatg tgctcaatta aaaacacatt tttttacgtg catgccataa attatgcgtg
    14221 attcgttgaa taaacaaatc tatttttgca ataattatat cccgatgacg cagcgctgca
    14281 taatcaatag atttaccata tatattattt aatttacctt tgattgaacc tctattatcc
    14341 aatttatctt tggttgcatc tttattttcg aattgatttg aattgagtgt tttattaaag
    14401 tatccagttg caccgggtat tatcttcaac atgattcatc tacctttttt gtcgtaccgt
    14461 gtcctcctta taattctttt gccatctggt cactatcaaa aatcgctcat aatttctgaa
    14521 ctacaggtat gcgtttaaac ccagttaaat aaatagcgag ttaacttgaa gcaatttatt
    14581 taaaaatgaa ttattaatta ttatcgtgtt gataatggta cgataaggca acacaacacc
    14641 ttacctacaa aacagaaaat caacattacg gcaacaataa gggttctcgg tgtttttcct
    14701 ggaacatggt aaagtaagga cattcttaac ccccactttg aggtgcccga tggaaaagac
    14761 cacaacgcag gagttattag cgcaggctga aaaaatctgc gcgcagcgta atgtgcgcct
    14821 gaccccacag cgcctggaag tgttgcgcct gatgagtctg caggatggcg ctatcagcgc
    14881 ttatgatctg cttgatttac tgcgcgaagc tgaaccgcaa gccaagccgc caacggttta
    14941 tcgcgcgctg gattttctgc ttgagcaagg ttttgtgcat aaggtggaat ccaccaacag
    15001 ttatgtgctc tgtcatctgt tcgaccagcc cacgcatacg tcagccatgt ttatttgcga
    15061 tcgctgcggc gcggtgaaag aagagtgtgc agaaggcgtg gaagacatta tgcatacgct
    15121 ggcggcaaaa atggggtttg ccctgcggca taatgtgatt gaagcacatg ggctctgtgc
    15181 ggcatgcgtg gaagtggaag cgtgtcgtca tcctgaacag tgccagcatg atcactctgt
    15241 gcaggtgaaa aagaaaccgc gttaagaggg cgtacatcct tgtacacgtc gggcaggagg
    15301 gattaattac cagcgatatt cattgcgggt ttcccaatcc acgacctctt tttctgcctg
    15361 atctttctga taaccataac gttcctggat tttaccgacc agttgatcac gtttaccttc
    15421 aatgatcgtc atatcatcat cggtcagttt gccccattgc tctttcactt tacctttaaa
    15481 ctgtttccag ttaccgccgg cttcatcttt attcataatc aagacctcat cgttaggttg
    15541 tgaatgagag tacgttcact tttcttctga acgtgagatt aagtgtagtc agcaatttgg
    15601 cttaggataa tttattcaga atttttaacc gtcacgttgc ggcaaaccag gtaccgttgc
    15661 gccagtgacg ccgccagata gccgccagag aaagcccgcg caacgccaga aagacggtta
    15721 atgccagcca caaaccatga ttacccagcc acggcagcgt aaggagcgtc agcgcaaaac
    15781 ctgcggcggc caccgccata ctgttacgca tttcggcggc acgcgttgcg cctataaaca
    15841 tgccgtccag cagataacac cagacgccaa ccaacggcaa aatcacctgc cagataagat
    15901 agcggtcagc cagctgctga atctgggtta acgacgtcag caacgcaatg atgtgttccc
    15961 ctgccagcaa ataaaccacc gaaaatagta acgctacaat ccccgactgg cggcacgctg
    16021 cccgccatac atccagcaac tggctaccgt cgcgcgcacc gtatgcctga ccggagtgcg
    16081 cttcaaccgc gtaggcaaaa ccatcaagcg cataggcggt aaaggtgagt agcgtcatca
    16141 gaaccgcgtt aacagcgata atgtcactcc ccagtcgcgc accaagtacg gtgatcgcgc
    16201 cgaaacagag ttgcaacaac agcgaacgca gcatgatatc gcggttaagc gccagcaagc
    16261 gacggaagtt tcctcgccag gcagttttca gcatttcgcc ggaaattccg cgtagtttga
    16321 ggattttacg caccattagc agaccaatca gcaatgttgc atattccgca ataaccgtcg
    16381 ccagcgccgc gccctgcacg ttcatatgca gccccatcac cagccagaca tccagcacaa
    16441 tgttgaggat attgccgacc actaacaaaa ttactggcgc acgggcatat tgcacgccga
    16501 gtaaccaacc gagtaatacc agattcgcca gcgacgccgg agcgcttaac cagcggattt
    16561 caagaaagcg ccgcgcctgt tccaggaccg cttcactacc gccaacaata tgcagcgcca
    16621 gatcgataat cggcgtacgc aacagcgcaa ttaacgcccc ggcccccaac gccaacagca
    16681 acggttgcac cagcgcacgg gctaatgcct gaggattttt ggcaccataa gcctgcgcag
    16741 tcagcccggt ggtgctcatg cgtaaaaaca gcaacagcat aaagagaaag ctggtcgccg
    16801 ttgcaccaac cgccacgccg cccaaataaa ccggactatc aagatgacca attaccgccg
    16861 tatcgaccag tcccagcaac ggaacggtga tattggagaa aatcatgggt aaggcgagat
    16921 gccagagtgc tttatcagat gaagtgagga atgccatgca gacaagcctg atgaagagag
    16981 atgaaaaaca aaccgcgacg ccaagcggca tcgcggtctc agagatatgt tacagccagt
    17041 cgccgttgcg aataacccca accgccagcc cttcaatggt gaagctctgc tgacgaaggt
    17101 caacgacaat tggtttaaac tcgctatttt ctggcaacag ttcgacttta ttgccctgtt
    17161 ttttcaggcg cttaacggta acttcgtcat caatacgtgc gacaacgacc tgaccgttac
    17221 gtacatcctg agttttatgc actgccagca agtcgccatc cataatgccg atatctttca
    17281 tcgacatccc gctgacgcgc agcaggaaat cagcattcgg cttgaacaag gaaggatcga
    17341 cctgataatg accttcaata tgctgttgcg ccagaagcgg ttcaccggca gccacacgac
    17401 ctaccagcgg caacccttct tcctcttcct gcaacagacg aatcccgcgt gatgcgccgg
    17461 aaacaatttc aataacgcct ttgcgtgcca gcgccttcag atgttcttca gccgcgtttg
    17521 gggaacggaa ccccaaacgc tgcgcgattt ccgcacgcgt cggcggcata cctgtctggc
    17581 tgatgtgatc acggatgaga tcaaacacct cttgttgcct ggccgttaac gctttcattc
    17641 cgccccctgg gtgtatatac agttatactg tgagtatata cagcaaaagg cgattttgga
    17701 accataaact gcacaataaa ccagagattt atcgaaatct ggaagggtta tccaaaatgc
    17761 gaccataaca ggatgcacca ggtaatcacg gcgacgataa tggcaatcag caccgcggcg
    17821 gatcccatat cttttgcgcg tccggaaagc tcatggtatt cagagccaat tcggtcaacc
    17881 actgcttcga tggcgctatt gaggatttcc acaatcatca ccagcatcac ggagctgata
    17941 agcagcacgc gggtaattgc gtccacatcc agccagcagg cgatgaccac cgccaacaat
    18001 accgctacgc cttcctgacg gaatgccgct tcgttgatcc atgcagcgcg taaacctttc
    18061 caggaatagc cagcagcttt gataattcgg gtgaatccag ttgtattatt ggccattgaa
    18121 agaacctttt tacattatga gcgtcaatat cagtgtaccg ttaaatccga tgattcgggt
    18181 ttgcgtaacg ccgcgcgaaa catgagcgga taccacagaa tttcccatga ctttctgcta
    18241 tccttgccgc gcatttgcat tattaaccag aggctttaca tcgtttatgt ccggctggcc
    18301 acgaatttac tacaaattac tgaatttacc attaagcatc ctggtaaaaa gcaagtctat
    18361 tccggcagat cctgccccgg aactggggct ggatacctct cgtccaatta tgtacgtttt
    18421 accgtacaac tcgaaagcag atttgctgac gttgcgcgcc cagtgtctgg cacatgattt
    18481 gcctgacccg ttagagccgc tggaaatcga cggcacgcta ctgccgcgct atgtgttcat
    18541 tcacggcggg ccgcgtgtat tcacctatta cacgccgaaa gaagagtcta ttaagctttt
    18601 ccacgactat ctcgatttgc accgtagcaa cccaaatctg gatgtgcaga tggtgccagt
    18661 atcggtgatg tttggtcgcg cgccggggcg tgaaaaaggc gaagtgaacc cgccgctgcg
    18721 tatgcttaac ggcgtacaga aatttttcgc tgtactgtgg ctcggtcgcg acagttttgt
    18781 gcgtttctcg ccgtcagtgt cgctgcgccg tatggcggat gaacacggca cggataaaac
    18841 tatcgctcag aaactggcgc gcgtggcgcg tatgcacttt gcccgtcaac gtctggctgc
    18901 cgtaggccca cgtctgcctg ctcgtcagga tctgtttaat aagctgctcg cctcccgcgc
    18961 cattgccaaa gcggtagaag atgaagcgcg cagcaaaaaa atctcccatg aaaaagcgca
    19021 gcagaacgcg attgcgctga tggaagagat tgcggcgaat ttctcttacg agatgatccg
    19081 cctgactgac cgcattctgg gcttcacctg gaaccgattt tatcagggca tcaacgtcca
    19141 taacgccgag cgcgttcgcc agctggccca cgacggtcat gagctggtgt atgtgccttg
    19201 ccaccgcagt cacatggact acctgctact ttcttacgtg ctgtaccacc agggactggt
    19261 gccgccgcat atcgccgccg ggatcaacct gaacttctgg ccggccgggc cgattttccg
    19321 ccgtctgggg gcgttcttta ttcgccgtac ttttaaaggc aataaacttt attccaccgt
    19381 tttccgtgag tatctcggcg aactgttcag ccgtggttat tccgtcgagt acttcgtgga
    19441 aggcggtcgt tcccgcacgg ggcgtttgct ggatccgaaa accggtacgc tgtcgatgac
    19501 cattcaggcg atgctgcgtg gcggcacgcg tccgattacg ctgattccga tctatatcgg
    19561 ttatgagcac gtcatggaag tgggtactta cgccaaagaa ctgcgcggcg cgacgaaaga
    19621 gaaagagagc ctgccgcaga tgctgcgcgg tttaagcaag ctgcgtaatc tcggtcaggg
    19681 ttacgtcaac ttcggtgaac caatgccgtt gatgacctac cttaaccagc acgtaccaga
    19741 ctggcgtgaa tctatcgatc ccatcgaagc ggtgcgtccg gcctggttaa cgccgacggt
    19801 caataatatt gctgccgatc tgatggtacg cattaacaac gcaggcgcgg caaacgccat
    19861 gaacctgtgc tgtactgcgc tactggcatc acgtcagcgc tcactcaccc gcgagcagtt
    19921 aaccgagcaa ctcaactgct acctggatct gatgcgcaac gtaccttact ccacggactc
    19981 taccgttcct tcagccagcg ccagcgagct tatcgatcac gcgctgcaaa tgaacaagtt
    20041 tgaagtcgag aaagacacta tcggcgacat catcattctg ccacgcgagc aagcggtgct
    20101 gatgacctac tatcgcaaca acattgcgca tatgttggtg ctgccttcgc tgatggcggc
    20161 aattgtcacc cagcatcgcc acatctcccg cgacgtattg atggagcacg tcaatgtgct
    20221 ttacccaatg ctgaaagcgg agctgttcct gcgctgggat cgcgacgagt tgccggacgt
    20281 tattgatgcg ctggcaaatg agatgcaacg tcaggggctg attaccctgc aagatgatga
    20341 gttgcatatc aacccggcgc attctcgcac cctacagctg ctggccgcag gcgcgcgcga
    20401 aacgctgcaa cgttatgcca tcaccttctg gttgttgagt gccaacccgt cgatcaaccg
    20461 cggtacgctg gagaaagaga gccgcaccgt tgcgcaacgt ctctccgtgc tgcacggcat
    20521 caacgcgccg gagttcttcg acaaggcggt gttcagttct ctggtgctga cgctgcgtga
    20581 tgaagggtat atcagcgata gcggcgatgc cgaaccagca gaaacgatga aggtttatca
    20641 gttgctggcg gagttgatta catcagacgt gcgtttgacg attgagagtg cgacgcaggg
    20701 cgaagggtaa tcagagagaa ttgccggatg cggcgaaaac gccttatccg gcctaccatg
    20761 acctgcaaat tcaataaatt gcgattcacc gggtaggcct gagaagcgca acgcatcagg
    20821 caacccagaa gaaagccgga tgatcatccg gcttttttac atcatcagaa atgccagtaa
    20881 ctcattgcca gtcctaaaaa cagtaccaga ccaacatagt tattattcat aaatgcttta
    20941 aagcaggctt cacgctcgcg gttggcaatc agtttttgtt gataaacaaa cagcgcgcca
    21001 gccaccacaa ttgaccagta atatccccag cctaagccat ttaactcacc gatgatcgcc
    21061 atcagtgcca gtacgccaat ctgcaaaata ccaataatca atttatcgta ttggccgaac
    21121 aggattgccg tggatttaat gccaatcttc acatcatcat cgcggtcaac catcgcatac
    21181 tgcgtgtcgt aagccaccgc ccagagaata ttggcgagga acattaacca gcaactcaat
    21241 ggcaccgact cactcacagc ggcaaaagcc attggaatcg accagccaaa cgccgcgccc
    21301 agcaccactt gcgggagatg ggtataccgc ttcataaacg ggtacaccca cgccaatgcc
    21361 agagcggcaa tcgacaacag aatggtcatc gtattcagcg tcagaaccag taaaaacgaa
    21421 atcagtacca gcactacaaa cagcgcgcgc gcctctttct ctgttaccgc gccgctggga
    21481 agtggtcggt tcgccgtgcg cttaacatga ccatcaaact tgcgatcagc ataatcattc
    21541 accacgcaac cagcagcgcg catcagccag acacccgcga caaacaccgc catgatccag
    21601 agctgaggaa cgcccggtgt cgccacccac aacgcccata atgttggcca gagcagcagt
    21661 aacgcgccaa ttggcttatc cgtacgcatt aagcgatgaa acgccagcag cttattctgc
    21721 gtcagactcc actccatatt tttttcctct tagtacaacg gtgacgccgg taaaaacagt
    21781 tctgttagca acagcggttt accgcttaat cgcaggcggg aacgtcgccc ccacagtccg
    21841 gcatcacggc ctatctcgat aaaatcccgg gttagtgtcg atgatgtgaa cagatagcgt
    21901 cctaacggcg ttttacccaa tttttgtaac gctagttccg gcccgcttaa tgttgataca
    21961 ggaacgacgg tacgaccggc aagccacggt tcaccatcgg cacataacaa aatttcacgt
    22021 aaccagtaac gagactcttt cggcagcagc ggcagttctt cggggatttc attctgctcg
    22081 acaaacccct cgcggatcat cgtcacactc accgtttttc cctgctgttc aaaacgtttt
    22141 gtcatggaat cctccagcaa cagccagtcg agcagttgcg gatccagggc agggatctct
    22201 ttaaaatagc gcagcgcacg cagttgcgtt aacgcggggt gtgacatgcc gaactctccg
    22261 ttacataacg taaaggcatt gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt
    22321 aagtgatcta atgcgcaaca aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa
    22381 ccttacgctt tgccgatgcc cgtgctggcg agggcaacca taggggcatc accgcccatg
    22441 atgctaggaa cggtgatgtt ttcctttact taaatacctt tattctttat acgagtagct
    22501 cgccaactcg cagtaaaaat tgggaaaagc gcatatttac catgagattt ggagctgaaa
    22561 agtgtttcct gagtaagatt aataggcaac atcttatgtc tggatttata gatacacgtg
    22621 gaattccaat tggtatcttt tgtaggattc aacatatcat gtaagcgctg ggagcttacc
    22681 accataacag gcccatcatc gacagaggag aacaggataa atatattatc gtttcgtgtt
    22741 aatttattag tgttaaaggt tcttaacttt tcttcagttt ctataaatat aggtgaaaca
    22801 tcatcactac gaatccactc tttataaaga taccaggcat ttgctttgat catatcatcc
    22861 gttgatcgta aggcctgatc gataagttgg ataaaaaaat tgttattttt atcaaccagc
    22921 cctttattca tattaaatct gttaataaaa tggatcatct gttgggtatc gaaattcacc
    22981 ttactgaatc gatgttgacg cataaaatta tcgaacttgt catcttcccc agaatgtata
    23041 taactacgta taattggatc ttttatataa ggacaatggc taaatgtttt cttcatattc
    23101 tcaataaagt ttttattaac aatattgaga atttttaatt gcatcaccag ttttactgcc
    23161 agaacggatt tcaatatcat tgcattatct ggcaatgtat caatcatttt tataatgtta
    23221 ttgatataac catcgagatc ttcattccca aatatatctg aataatcaat agttaattcc
    23281 aattgttctg taatgcaatc ttaaaaattc cctgtcgaca ttcgcacatg atcaaacata
    23341 acagctgtca aattcgattt aataaatgat atttctttgg cagacagaaa ctctgctctg
    23401 gtgaagtgcg aagcgtaaag ataacaatta tcgaagtttg aatttctaat ctgacattcg
    23461 cgaaaattac tatgaccaag ttgactctgc gagaagtcaa ctgtatcaag aatattttgg
    23521 ctaaaatcaa cacattcgcc actcgttttg agaaaaatca cattgtgtaa acgtcgacat
    23581 ttaaatatga cattatcaac tgttgcctta gtaaaattgc aattctcaat ttctttacat
    23641 tcgaaagtta catgttgtag atttgatgat aaaaaagaca ccttagaaat aacgtcacct
    23701 ttaaaatgac aatttgttaa atcaaaatca gacaggtcga tatacccctg cgcatcttta
    23761 tccatcagaa aattacaagc gcattctatt ttttcatttc tgtagaattt tacacttata
    23821 gtatcattac taattgagac ttcacgtaac atatttattg catgattttt ttcgttccgt
    23881 tgttttatat ttaaaaatat atcactgata taaataaagt aatctctgtt taaacctgtc
    23941 accccattga aattacgtag taaatcaaga tctactatat tatttccttg caccacatca
    24001 tgtttcatca aagagttatt gccgccagaa tttgtgatat gacctatcat aaaacctctg
    24061 taatacagaa ataaaataac ctggaaaagt agcctgagcg tccagacact accggcgaaa
    24121 ggaaaagaat ctctgataat gcattgaaat aatggacatt cttagtgaag aagaagtgaa
    24181 gtactataaa aaatcgctca tatcccagga aggaagcata aaaaaaggtg cgccaggaga
    24241 cgcaccagtt gttgcaaaat cagcactgtg gggcgtaatt accccttgcc ttttacactg
    24301 ctgataaagg tggaacgggc agatgtcgat cccaggcgtt cagcttcatc aagcagtttt
    24361 aacgccttat caacatcacc tttcgcgaca gcgtttttga tcgcggtatt aaaataactt
    24421 tccgtgtcgt tgagcatcgg ctcgcttttc ttcaccggca ccggagcagg tgcagccaca
    24481 gctggtgccg ccgtgttgcc taccgtaacc ggagctggag cggaagaacc aaatagcggt
    24541 cctaccaaca cgctggagct ggagttcgtt ttgactttca gtttcagtaa gccatcgctg
    24601 gtatgacgag caaccggatc gggaatatcc gggatcgagt taccgacgcc tttggcatag
    24661 gctttagccg ggtcgagcag ttgggtcgtc tgctggagat ctttttccgt ggtaaagacc
    24721 agaacataaa gtttttgctg ccccaacgcc ggtgtcaggc gcataacgcc ttccagccga
    24781 tctgcactca tcacgcctag ttcctggtag gtgaaataac tgctggggaa gaaggctgat
    24841 ggggtcatgt tctgatcaag aatcagcacg ttcggcgcaa aaacgctggt ttgtttgttc
    24901 acttcgctgg tcagcgtcag ggtcagttcg ccaatgtttg ccgggacgct gtacgcagca
    24961 accggaccac tgatgccggg aacgttcagt tgttggccgc cggtcgccag ttgggtggtc
    25021 tgggttttag attgatcgac cggtgtccag gtgagttgtt gcagcgcggc agatggaatg
    25081 gctggcgcgt cgctggtgtt ttgcggtacg tagttaacat cggcaaggct aattccaggc
    25141 gcgcttgcca gtaaccctgc tgataaacag agggcgatga gacttttatt cattttcatt
    25201 gttttcacct caaaatctgg agctcagcgg tagccaggca atagcgcgct aaacccgata
    25261 atcagagggg ctttcgcccc tttcagataa tgacaacctg tttttatgcc ggatgcggcg
    25321 taaacgcctt atccggccca ggttttgcta ttaccaccag atttccatct gggcaccgaa
    25381 ggtccactcg tcgctgtcgc cacgaccgaa gctgccgccg ttgaaatcag caggaacggc
    25441 tttgccgtag ttcgggttaa ccttgctatc gccgttgtag tcgtaacccc atttctcatc
    25501 ccacttggcg taggttgcga agacacgaat agccgggcgt gaccagatgc tgtcgccagc
    25561 ctgccattgt tgtgcgaggg taattttgta ctgattgttc ttgtcgccgg tgcgctggga
    25621 ttcgacgttg tcgtagccga tttccatcac ggtgctcatg attggcgtcc acttgtacat
    25681 cgggcgaata ccaacggtcc accacttggt gccgttgtcg ttatcccagt tgatatcctg
    25741 gtacataccc acgtacatca tgtcccagtt gtcgcccatg gagatcgcac cgtggtcgag
    25801 gatacgcagc atgtgaccgt tgttgttgat attgtaggca aatttttcgt tatcaaacgc
    25861 gacgccagaa ccctgcgaca gacctttacc ctgcgaggtc atcgagtcag tagcgtactg
    25921 aacaacaaac ttgttaaagc ccttcaggac actctgagta tgttcagcag tgaataacca
    25981 gccgtctttc gatgcgccat caaccagacg atagttatca cgcaggttgg cacgaccgta
    26041 gtcgacaccc agttctaatg tgccgcccgg gttgatttcc atctgcgcta aacgcacatc
    26101 gaaaacgtcg ttcgcggttt cgttggtata gtcataaata ttgttgctgg caaaagagga
    26161 agaaccacca gcttcagagg agcgggttgc tgccagagag agtttaccga agccaacatc
    26221 gatgttttcc agaccggcac caggaccaga aatatcccag tagtagaagt cgatcatatg
    26281 aacgtcatga cgttggtaga agcgcttacc tgcccagatg gtggaacctg gcagccattc
    26341 gatcaggttt tttccctgca cgtttgcttc acggaaggcc ggatcggtag cttcccagtc
    26401 attctgttgc gcgacggaat aggccacgtt agtgtcgaaa tagaagctct tatcgccctc
    26461 tttccacact tcctgaccca attttaattc agcataagtt tcacattcgt tgccaagacg
    26521 gtatttactt tgagcaccgg tagtctggaa acactgttgt tcaccgccgc tacctgtcca
    26581 gccaataccg gaacgtgcat agccgtggaa atcaacagcc attgcctgag cagacattac
    26641 gcccgctgcg acggcaaccg ccagaggaag tttgcgcaga gtaatcatca ttctatctcc
    26701 tgagtcattg cttttctttt ttcacatcac ctgtgacagg ctttgtgtgt tttgtggggt
    26761 gcttaaacgc ccggctcctt atgcagtcga cgacatgcag tgccatcctc acggaacaga
    26821 tggcaacgct ctggcggcag gccgatagcg aatgtggcac cttcttctac caacaccacg
    26881 tcgttctggc ggtacaccag gttttgacga atggaaggga tctggatata gatttgggtt
    26941 tcgttgccga gttgctcgac gacctgaact tcaccctcaa ggatgacgtc agcgatatca
    27001 ctcggcagta gatgttccgg gcgaataccc agcgacatat tggctccaac ctggacatca
    27061 cggctttcaa caggcagcca gacttgttga cgatttggca tcggcagctc cacctgcact
    27121 tgatcgattg cggtggcggt cacttttacc ggcaggaagt tcatctttgg cgaaccgata
    27181 aatccggcga caaaacggtc tgccggatag tggtacagtt ccagcggttt cccaacctgt
    27241 gccacgcgac cggcgtccag caccacgatt ttgtcggcca gcgtcatcgc ttcgacctga
    27301 tcgtgggtga cgtaaatcat tgtgcggccc aggcgtttat gcagacggga gatttcgata
    27361 cgcatttgca cacgcagtgc agcatcgagg ttggagagcg gttcatcgag caaaaatacg
    27421 cttggctcgg ccaccagcgt acggccaatc gccacgcgct gacgctgacc accggagagc
    27481 gctttcggtt tgcgatccag caaatgcgcc agttgcagca cttccgccac ctggttgacg
    27541 cgttggttaa tcacctcttt ttttgcgcca gccagtttca ggccaaatga catgttttct
    27601 gctactgaca ggtggggata gagcgcgtaa gactgaaaca ccataccaac gccgcgttct
    27661 gctggcggag tgtcattcat ccgtttctca ccgatgaaca ggtcgccgct ggtgatcgtc
    27721 tcaagcccgg caatcatgcg cagtaaagtc gatttaccgc agccagacgg tccgacaaac
    27781 accacgaatt caccttcatg gatatcgaga ttgatatctt tcgataccac gacctcgccc
    27841 caggctttcg ttacattttg cagctgtacg ctcgccatgc ccttctccct ttgtaacaac
    27901 ctgtcatcga cagcaacatt catgatgggc tgactatgcg tcatcaggag atggcttaaa
    27961 tcctccaccc cctggctttt ttatggggga ggaggcggga ggatgagaac gcggcttctg
    28021 tgaactaaac cgaggtcatg taaggaattt cgtgatgttg cttgcaaaaa tcgtggcgat
    28081 tttatgtgcg catccccaca ttaccgccaa ttctgtaaca gagatcacac aaagcaacgg
    28141 tggggcgtag gggcaaggag gatggaaaga ggttgccgta taaagaaact agagtccgtt
    28201 taggtgtttt cacgagcact tcaccaacaa ggaccataga ttatgaaaat aaaaacaggt
    28261 gcacgcatcc tcgcattatc cgcattaacg acgatgatgt tttccgcctc ggctctcgcc
    28321 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
    28381 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
    28441 gataaactgg aagagaaatt ccctcaggtt gcggcaactg gcgatggccc tgacattatc
    28501 ttctgggcgc acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
    28561 ccggacaaag cgttccagga caagctgtat ccgtttacat gggatgccgt acgttacaac
    28621 ggcaagctga ttgcttaccc aatcgctgtt gaagcgttat cgctgattta taacaaagac
    28681 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
    28741 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
    28801 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
    28861 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
    28921 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
    28981 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
    29041 aattatggtg taacggtact gccgaccttc aagggtcaac catctaaacc gttcgttggc
    29101 gtgctgagca ccggtattaa cgccgccagt ccgaacaaag agctggcgaa agagttcctc
    29161 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
    29221 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccacc
    29281 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
    29341 tatgccgtac gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
    29401 ctgaaagacg cgcagactcg tatcaccaag taatgctgtg aaatgccgga tgcggcgtga
    29461 acgccttgtc cggcctacaa aactgaaacg catgtaggcc tgataagacg catcagtgtc
    29521 gcatcaggca gttgttgtcg gataaggcgt gaaagcctta tccgtcctgg aatgaggaag
    29581 aaccccatgg atgtcattaa aaagaaacat tggtggcaaa gcgacgcgct gaaatggtca
    29641 gtgctaggtc tgctcggcct gctggtgggt taccttgttg ttttaatgta cgcacaaggg
    29701 gaatacctgt tcgccattac cacgctgata ttgagttcag cggggctgta tattttcgcc
    29761 aatcgtaaag cctacgcctg gcgctatgtt tacccgggaa tggctggaat gggattattc
    29821 gtcctcttcc ctctggtctg caccatcgcc attgccttca ccaactacag cagcactaac
    29881 cagctgactt ttgaacgtgc gcaggaagtg ttgttagatc gctcctggca agcaggcaaa
    29941 acctataact ttggtcttta cccggcgggc gatgagtggc aactggcgct cagcgacggc
    30001 gaaaccggca aaaattacct ctccgacgcg tttaaatttg gcggcgagca aaaactgcaa
    30061 ctgaaagaaa cgaccgccca gcccgaaggc gaacgcgcga atctgcgcgt gattacccag
    30121 aatcgtcagg cgctgagtga cattaccgcc attctgccgg atggcaacaa agtgatgatg
    30181 agctccctgc gccagttttc tggcacgcag ccgctctaca cactcgacgg tgacggcacg
    30241 ttgacgaata atcagagtgg cgtgaaatat cgtccgaata accaaattgg cttttaccag
    30301 tccattaccg ccgacggcaa ctggggggat gaaaagctaa gccccggtta caccgtgacc
    30361 accggctgga aaaactttac ccgcgtcttt accgacgaag gcattcagaa accgttcctc
    30421 gccattttcg tctggaccgt ggtgttctcg ctgatcactg tctttttaac ggtggcggtc
    30481 ggcatggttc tggcatgtct ggtgcagtgg gaagcgttgc gcggcaaagc ggtctatcgc
    30541 gtcctgctga ttctgcccta cgcggtgcca tcgttcattt caatcttgat tttcaaagga
    30601 ttgtttaacc agagcttcgg tgaaatcaac atgatgttga gcgcgctgtt tggcgtgaag
    30661 cccgcctggt tcagcgatcc gaccaccgct cgcacgatgc tgattatcgt caatacctgg
    30721 ctgggttatc cgtacatgat gatcctctgc atgggcttgc tgaaagcgat tccggacgat
    30781 ttgtatgaag cctcagcaat ggatggcgca ggtccgttcc agaacttctt taagattacg
    30841 ctgccgctgc tgattaaacc gctgacgccg ctgatgatcg ccagcttcgc ctttaacttt
    30901 aacaacttcg tgctgattca gctgttaacc aacggcggcc cggatcgtct tggcacgacc
    30961 acgccagccg gttataccga cctgcttgtt aactacacct accgtatcgc ttttgaaggc
    31021 ggcgggggtc aggacttcgg tctggcggca gcaattgcca cgctgatctt cctgctggtg
    31081 ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga agtttgatta agggagataa
    31141 caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg tttgtttatt actcacctgc
    31201 tactgctact ttttatcgcg gcgattatgt tcccgctgct gatggtcgtc gctatctcgc
    31261 tgcgtcaggg aaacttcgcg accggtagcc tgatcccgga gcaaatctcc tgggatcact
    31321 ggaaactggc gttaggtttt agcgttgaac aggctgatgg tcgcattacg ccaccgccat
    31381 tcccggtact gctgtggctg tggaactcgg taaaggtcgc tgggatttcc gcgattggca
    31441 ttgtggcgct ctccaccacc tgcgcctacg ctttcgcccg tatgcgcttt ccaggcaaag
    31501 cgacgctgct gaaaggaatg ctgattttcc agatgttccc ggcagtactt tcactggtcg
    31561 cgttgtatgc gttgtttgat cgtctgggtg agtacattcc attcattggc ctgaatactc
    31621 acggcggcgt aattttcgcg tatctgggtg ggattgcgct gcatgtctgg accatcaaag
    31681 gctatttcga aaccatcgac agttcgctgg aagaagcggc tgcgctggat ggtgcgacac
    31741 cgtggcaggc cttccgcctt gtcctgttgc cgctgtcagt accgattctg gcggtggtat
    31801 tcatcctgtc gtttatcgct gccattactg aagttccggt cgcgtcgctg ttactgcgtg
    31861 acgtaaacag ctacaccctg gccgtgggga tgcagcaata cctcaacccg caaaactacc
    31921 tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt accgatcacc atcgtcttct
    31981 tgctggctca acgctggctg gtcaacggcc tgacggcagg tggtgtgaaa ggttaaagat
    32041 gttgttctgc caatgttatg ccgctgcacc ctcaacttac gttatcccaa cttgtgacta
    32101 ttattcggcg ctccacggag cgcctttttt tctttcgtct gcaatctgaa tcgttcgccg
    32161 gttaatattc ccatcataga acttattatt tttacgttat ttgttttccc acttacgata
    32221 attctctttc gtgctctgag tcacggcaat agtattgttt catcaatttt tgataattat
    32281 cacaattaag atcacagaaa agacattacg taaacgcatt gtaaaaaatg ataattgcct
    32341 taactgcctg acaattccaa catcaatgca ctgataaaag atcagaatgg tctaaggcag
    32401 gtctgaatga atacccagta taattccagt tatatatttt cgattacctt agtcgctaca
    32461 ttaggtggtt tattatttgg ctacgacacc gccgttattt ccggtactgt tgagtcactc
    32521 aataccgtct ttgttgctcc acaaaactta agtgaatccg ctgccaactc cctgttagga
    32581 ttttgcgtag ccagcgctct gattggttgc atcatcggcg gtgccctcgg tggttattgc
    32641 agtaaccgct tcggccgtcg tgattcactt aagatttctg ctgtcctgtt ttttatttct
    32701 ggtgtaggtt ctgcctggcc agaacttggt tttacctcta taaacccgga caacacagta
    32761 cctatttatc tggcaggtta tgtcccggaa tttgttattt atcgtattat tggcggtatt
    32821 ggcgttggtt tagcctcaat gctttcacca atgtatattg cggaactggc tccagctcat
    32881 attcgcggaa aactggtttc atttaaccag tttgcgatta ttttcgggca acttttagtt
    32941 tactgcgtaa actattttat tgcccgttcc ggtgatgcca gctggctgaa tactgacggc
    33001 aggcgttata tgtttgcctc ggaatgtatc cctgcactgc tgttcttaat gctgctgtat
    33061 accgtgccag aaagtcctcg ctggctgatg tcacgcggca agcaagaaca gacggaaagt
    33121 atcctgcgca aaattatggg caacacgctt gcaactcagg cagtacagga aattaaacac
    33181 tccctggatc atggccgcaa aaccggtggt cgtctgctga tgtttggcgt gggcgtgatt
    33241 gtaatcggcg ttatgctctc catcttccag caatttgtcg gcatcaatgt ggtgctgtac
    33301 tacgcgccgg aagtgttcaa aacgctgggg gccagcacgg atatcgcgct gttgcagacc
    33361 attattgtcg gagttatcaa cctcaccttt accgtactgg caattatgac tgtggataaa
    33421 tttggtcgta agccactgca aattatcggc gcactcggaa tggcaatcgg tatgtttagc
    33481 ctcggtaccg cgttttacac tcaggcatcg ggtattgtgg cgctactgtc gatgctgttc
    33541 tatgttgccg cctttgccat gtcctggggt ccggtatgct gggtactgct gtcggaaatc
    33601 ttcccgaatg ctattcgtgg taaagcgctg gcaatcgcgg tggcggccca gtggctggcg
    33661 aactacttcg tctcctggac cttcccgatg atggacaaaa actcctggct ggtggcccat
    33721 ttccacaacg gtttctccta ctggatttac ggttgtatgg gcgttctggc agcactgttt
    33781 atgtggaaat ttgtcccgga aaccaaaggt aaaacccttg aggagctgga agcgctctgg
    33841 gaaccggaaa cgaagaaaac acaacaaact gctacgctgt aatcttcctg tccagcacgc
    33901 cgcgccattt cggcgtgctg tctttttact cccgcttcag ccgtttcgaa ttacacagcc
    33961 acagggtgat caccagtaac aggatcgcag ccgagtagat caacacatcc agtggcgatt
    34021 tatgatcgac gatgatcaag cgcacaatgg cggtgatccc aatatagaca aagtaacgta
    34081 gcgggaagtg aaaaccggac tgaaagtact tcacaatcag cgcgataaat tcgaaataga
    34141 gaaagtaaac caccagtcct tctaccagct catatttgct ggtttgttct ggcgcgaaca
    34201 gcacatcagc cagatgcacc gtttctttgc cgaggaagac aaccaaaatc aggccgaggc
    34261 acagcaggcc aagattgagt acggtctgca aaatggtgga gataaactcc acgcgcggac
    34321 gagagagtga cgtcatagca gtacctcctt cacagtggcg agcttcctgt ataacgcaaa
    34381 aatgtgacgg agatctatat tttttgtgta tgttttgttc aacctggatt tggagcgcgc
    34441 ctccaccaaa gccggatata cctacccgta ggccggataa gccgcgcctg gcggcgcatc
    34501 cggcaagaca aaccgcacca aagccggtaa ttcaaaaatc acccattcag gtgattccca
    34561 gccaccagca atgcgcgaaa aatcgacaat gttcctctgg aacaaatgaa acgtaacctg
    34621 ataaaggtgg taaaaatgaa gccttatttt gctgctttga tgttatcagt ctctgttctt
    34681 cctgcttatg ctggtccgct cggtactgct gataaagccg acctaccgca aagcaatgtc
    34741 tccagcccaa tgatggccca gtcgctcagg caaccagacc tgcaaccgat atcgacggac
    34801 aggaagacag aatgttttcg cctttacaca ccagatcgta aaccgggggt gaactgcgtt
    34861 cctgatggct caacaggtca ttaagaatga gggtgccagt gagtgcgaaa actggcacct
    34921 ttcccatgac ttagcggaaa tttacgctac ggtcgctggt catgtcatac aactggaact
    34981 tacgcccaag ttgctgacca ccgtcacgcg tcagcggcgt ccagccaatc gccgcacggc
    35041 tgcgtgtcgg gccagacgag aagagatcca acggtaccga gacatacacg cctttggtga
    35101 agtccccttc gccgtactcc tctttcgaaa cgttggtgat cgtggcatag ccacccacca
    35161 ccacgccgct gtcaaagcgt ttagcgatct ccagcgtgcc gcctttatcc cctgccagat
    35221 actgcccgac gctggctttc accaacacgt cctgggcgaa agatggcgtc cagtaggcgg
    35281 tcagatgtcc ggttttcacg ctgtagtcgg tgaatttcat catatctttt gcactacgcc
    35341 agtcgcgctg tttaacgtag ttggcatcca gaccaaacgc ccagttactg tccagcggac
    35401 gatacaacac ttcggccccc gcaccgccaa acatggtttc gagataacct ccatagacct
    35461 gaccgtagaa gccgttgccc agatgctgga agtagttggc ttgcaggtta ttcacataga
    35521 catcgttctg cacatactcg cgcacatggg tacgaacgcg cggtaagtgc gagtcctgcg
    35581 gtggattggt gtagttaaac ttgtcgtagt tgttggcaag atttgcaaac aggctgccgg
    35641 tggtcagtag atggtccgtc agccacaaat ctgccgttcc catcacgccc agctgataca
    35701 tgtaaaagtt ttccgggcca ccgaccgact ggttcagcac cggatcgata tggaaatcaa
    35761 agcgtgattt gtcgatatac cagccctgct cggtggactt cggaaccact ggctcgacgc
    35821 gtttgtgcgc cagcgtcgtt tcgtggccca acggctctcc ggcgagatga tttttcaggc
    35881 tggcgacatc ggtttccgtc gtcacttgcg gcatgttaag gcgattttcc gtaatgcgga
    35941 tcgttttgat cccatccggc agatcgttca tcacgatccg attagcacgg atgatccctt
    36001 cgcgcgaatc acgatatttc acctgctcgc cggtaacata cagcgtatcg cctttcgcct
    36061 ggatctgcgg atcggcaagt ccggcgttgt atttcagcaa cgttaactga ttcgccacca
    36121 ccgaatgctg caaaatggca tcctgcggct gcggttgata ttgcgggcgg gcgttatcga
    36181 tgtaggacgg gcgcagatcg ttaaagttgg tgcgcagcgt gacgccaaac ataaaggtgt
    36241 tgccacgttc atagctaagg ttaacgtcgg cccaatcggt aacgcgataa atcgccccga
    36301 cgttaaactt acttttttgc tccagcttcc cggcaaaatc ctgctgataa ttattacctt
    36361 catactccag tttcagacgc agcggttgcc agggcgtctg gtattccacg ccgccaaaca
    36421 gtgaggcagg accgtggaac atctggctac cgtcgataga tcccgcctgt ttgtagctgt
    36481 tatcgcgata gcaatattta tcactggctg aacagagcgg attttttaca ttaccgctgg
    36541 tgcccagata tccccagccc aggccgagcg taaaatcgaa cggcccccag gctttgctgg
    36601 caacaagata ttccgcatca aacagccccg taccgccgat atcccgcgca ccaaccgcca
    36661 cttgcggcag ccagtaactc tcttcccaca aacgcagttt gagatcgaag gctttatctt
    36721 tatacgtttg atcgccagag aacgcttcga cgctgctgta ctgccgggtg cgcacgtcgg
    36781 tgtagcgcag cgttgtttcc agccacggga agagttgcac tgaagctgag taataacggt
    36841 actgatcgtt atcgcgatag ttcagactca actccccttc ccgtgccata cgcgcggtgg
    36901 gagtttgtaa taatcctacg ccaccgaaat ccgactgcga cggaccaatg ggcgcaggat
    36961 atgtttcgcc gtagcaagct gtgctaatgc ccagcgccag taagctaagc agatgtcttt
    37021 ttttcattat tggggtatcc gctgcgtcag agtctgaaga atgtcggcgt taagggcatc
    37081 aggcgtctca ctccagacgg aatctgcgag gccaacataa ataatgctgc cgggcatcgg
    37141 ctctacgtga cgcttgttcc agtaagccac cggcgctttt tgcgtgcgtc cgtccgggta
    37201 aacgacccac gcgtagctgc gatccgcacc gctgagcagg ctctggtcag agagatagct
    37261 cgccacgtcg cgacctggag tgaatggctg attgccagga tggctgataa gcccgaacaa
    37321 cgtgaccgtg gacggcggtg gtccgaccca cagcgtgtag ttgccctgca acggcgggtt
    37381 accgcgttcg gcaacgcgga cgatatcggg atcaagattg attttttgcc tgcctgtcac
    37441 tttcaatgcc tggatttgct ggcgtaaggc gttaatggcg gcggcatcgt cggtgctgga
    37501 atctgcgccc tgttctgcca gccgtgtcag caacgcctgc tgctgacgca atgctgccgc
    37561 cgttgccagc tcttcgctaa tcaccgcacc aggccaccag ctgtttgcca gtcgaggttg
    37621 ccctaccaga tcgattaaat gctctgcgcc cgttagcgtt ttggcctcac tgctgccatt
    37681 gctgaacacc ttgacggttc ctgccgcgaa gaccgatgac gctcccacac tcaaaagcaa
    37741 cgcgacaata gtttgtttaa tcattgtttt gccgccttga tgagagtggt tttcaccggg
    37801 aaataatccg cgccgagata ctgttccgac tggcgaattt gcccttcgct gtcgatccag
    37861 tagcggttat gccagcgcgc ctggtcggtg gagacttctt cgtccagcac gcgaaccagg
    37921 gtttcatcgc tgccgacctt gacggtatcg gtgccatccc atttgaagac tgagcgtgcg
    37981 gtggcgtagc gtacctgctg gtactcggtc cagcccatcg tgcgcgtcca tgttgcacca
    38041 tcaacaattt gtgcgggttt aatcagcggg tcggcggcaa ggttattcac ttcaatcagg
    38101 ttgtcgccgc caagcaaagt cttcaccaga cgaccatgtt gtgtgacaag agtggcttga
    38161 tcctgagtga cccatttttg ttgtccgtct tcagcgaagg ccagcaccac aaacaattgc
    38221 ggcccgccat taagctgcat gtactggctg gcgtagggca tattttgaat atcatcgtcc
    38281 gtcagctgta cgcctggcgt gccgaacaga ctgtcccaca gtgaattgcc cagctcttta
    38341 gtcgtggctg aacaggcctg taatagcagg cagataagaa tgagtgcagg tcgcttcacg
    38401 actcttctcc agaggggcag aataaccaca ccggagtgtg gttatggtaa atcccttcat
    38461 acttcaagcc acagatgcaa tgactcaaat tattttgggg atacatactt tattactggg
    38521 tactggtggt cgtggttgtg gtggtcccgg tattagaacc atcaccgcca ccggttgccg
    38581 cgagcgcgac acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg
    38641 ccgaaaccga cgttgctgcc gactctgccg cttcgcccac ctgtacaggt gcagcccacg
    38701 cagaagtcgc cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa
    38761 tttcccttca ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata
    38821 cacaactaaa gcatgcgaat tacggcgcgg ggaattagcg atggagtttt aggataattg
    38881 gaatgaatat caaatataag tttcaactta tatttaaata acaataaaaa tcataagctt
    38941 aaaataaaaa acaaaagtaa ttaatctgcg tattctccga aataaacaac gattaaaaat
    39001 ctattttagg attaacctca cggtatgatt tccgttaaat cacagacaag gggatttatc
    39061 tgataaaaca atgccgactt attgtcggca ttgtttttaa ggttatttaa ctcctcatac
    39121 tatgcattaa tgtcgatatt ggcatcaggc tccggttgcc ggatgcggcg taaacgcctt
    39181 atccggccta catatcggca tcaggcacgc atgatgatta accgcgccac gctttatagc
    39241 ggttaatcag accattggtc gagctatcgt ggctgctgat ttctttatca tctttcagct
    39301 ctggcagaat acggtttgcc agttgtttac ccagctccac gccccactgg tcgaaggtga
    39361 agatgttcag gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgcac
    39421 ccaggctgaa cggagtgatt tcgcgcagca ggatggagtt ggtcgggcgg ttaccttcga
    39481 acactttgaa cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt
    39541 cctgctcaac cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt
    39601 tagacagcag tttctggtgg tgatcagaga gcgggttatg ggtgatagcc ggggcgatga
    39661 aatcgcacgg taccattttg gttccctggt ggatcagctg gtagaacgcg tgctgaccgt
    39721 tagtgcctgg ttcaccccag ataatcgggc cagtctggta atccacaacg ttaccgttac
    39781 ggtcaacata cttaccgttg gactccatat tgccctgctg gaagtacgcc gcgaaacggt
    39841 gcatatactg gtcatacggc agaatcgctt cagtttccgc accaaagaaa ttgttgtacc
    39901 agatgccaat cagcgccagc aggacaggca ggtttttctc ggcaggcgtg gtggagaaat
    39961 gcttgtccat tgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga
    40021 gaacaatcga caggccaatc gctgaccaca aagagtaacg gccgccgacc cagtcccaga
    40081 actcgaacat gttggcagta tcaataccaa actcgccaac ggctttggca ttggttgaga
    40141 gcgccgcaaa atgtttcgca acgtgttttt catcacctgc cgctttcagg aaccagtcac
    40201 gcgcgctatg ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga
    40261 acagcgtggt ttccgggttt acttttttca gcacttccga gatgtgagtc ccatcgacgt
    40321 tagaaacaaa gtgcatgttc aggtggtttt tgtacggacg cagtgcttcg gtcaccatgt
    40381 acgggccgag gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gctttgccgg
    40441 tataaccctt ccactcaccg gagataatcg cttccgagaa ggttttcatc ttctccagca
    40501 ccgcgttgac ttctggcata acatctttgc catcaaccag aatcggggta ttgctacgat
    40561 tacgcagcgc tacgtgcagc acggcgcggt tttcagtgcg gttgatcttc tcgccagaga
    40621 acatcgactt aatcgcgccc gccagatcgc actctttcgc cagatcctgt aatttcgcca
    40681 gcgtctcttc agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg
    40741 cggagaactt agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt
    40801 tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt
    40861 ttttcattag caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca
    40921 gtcacagaaa aatgtgatgg ttttagtgcc gttagtgtaa tgttgagtgt aaacccttag
    40981 cgcagtgaag catttattag ctgaactact gaccgccagg agtggatgaa aaatccgcat
    41041 gaccccgtcg ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta
    41101 gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat
    41161 aacacctgag ggggtgcatc gccgaggtga ttgaacggct ggccacgttc atcatcggct
    41221 acaggggctg aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt
    41281 ggtggagcac ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc
    41341 gctcttccct tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa
    41401 attgttgtct ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc
    41461 gctgatattg tgctttctga tgccaacgtg cgtttagtag tcctctcggc ttctgctggt
    41521 atcactaatc tgctggtcgc tttagcggaa gggctggaac ctggcgagcg attcgaaaaa
    41581 ctcgacgcta ttcgcaacat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt
    41641 atccgtgaag agattgaacg tctgctggag aacattactg ttctggcaga agcggcggcg
    41701 ctggcaacgt ctccggcgct gacagatgag ctggtcagcc acggcgagct gatgtcgacc
    41761 ctgctgtttg tcgaaatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt
    41821 aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg
    41881 gaactggccg cgctgcagct gctcccacgc ctcaatgaag gcttagtgat cacccaggga
    41941 tttatcggta gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat
    42001 acggcagcct tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc
    42061 ccgggcatct acaccaccga tccacgcgta gtttccgcag caaaacgcat tgatgaaatc
    42121 gcgtttgccg aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg
    42181 ttgctacccg cagtacgcag cgatatcccg gtctttgtcg gctccagcaa agacccacgc
    42241 gcaggtggta cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg
    42301 cttcgtcgca atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggc
    42361 ttcctcgcgg aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc
    42421 acgtcagaag tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat
    42481 acgttgctga cgcaatctct gctgatggag ctttccgcac tgtgccgggt ggaggtggaa
    42541 gaaggtctgg cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc
    42601 aaagaggtat tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc
    42661 agccataacc tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcaaaaactg
    42721 catagtaatt tgtttgagta aatactgtat ggcctggaag ctatatttcg ggccgtattg
    42781 a
//
LOCUS       DAJTRW010000026        40533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRW010000026 DAJTRW010000000
VERSION     DAJTRW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..943)
                     /locus_tag="OO326_001522"
                     /pseudo
     CDS             complement(1..943)
                     /locus_tag="OO326_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            1189..1815
                     /gene="gstB"
                     /locus_tag="OO326_001523"
     CDS             1189..1815
                     /gene="gstB"
                     /locus_tag="OO326_001523"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5965949.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1812..2926)
                     /gene="yliI"
                     /locus_tag="OO326_001524"
                     /pseudo
     CDS             complement(1812..2926)
                     /gene="yliI"
                     /locus_tag="OO326_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3037..3420)
                     /gene="bssR"
                     /locus_tag="OO326_001525"
     CDS             complement(3037..3420)
                     /gene="bssR"
                     /locus_tag="OO326_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5965950.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3633..4958
                     /gene="rimO"
                     /locus_tag="OO326_001526"
     CDS             3633..4958
                     /gene="rimO"
                     /locus_tag="OO326_001526"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5965951.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5005..6333)
                     /locus_tag="OO326_001527"
     CDS             complement(5005..6333)
                     /locus_tag="OO326_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5965952.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6341..8683)
                     /locus_tag="OO326_001528"
                     /pseudo
     CDS             complement(6341..8683)
                     /locus_tag="OO326_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8861..9772)
                     /gene="gsiD"
                     /locus_tag="OO326_001529"
     CDS             complement(8861..9772)
                     /gene="gsiD"
                     /locus_tag="OO326_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5965953.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9775..10695)
                     /gene="gsiC"
                     /locus_tag="OO326_001530"
     CDS             complement(9775..10695)
                     /gene="gsiC"
                     /locus_tag="OO326_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5965954.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10713..12251)
                     /gene="gsiB"
                     /locus_tag="OO326_001531"
     CDS             complement(10713..12251)
                     /gene="gsiB"
                     /locus_tag="OO326_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5965955.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12271..14142)
                     /gene="gsiA"
                     /locus_tag="OO326_001532"
     CDS             complement(12271..14142)
                     /gene="gsiA"
                     /locus_tag="OO326_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5965956.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14129..15094)
                     /gene="iaaA"
                     /locus_tag="OO326_001533"
     CDS             complement(14129..15094)
                     /gene="iaaA"
                     /locus_tag="OO326_001533"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5965957.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15298..16533
                     /gene="moeA"
                     /locus_tag="OO326_001534"
     CDS             15298..16533
                     /gene="moeA"
                     /locus_tag="OO326_001534"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5965958.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16533..17282
                     /gene="moeB"
                     /locus_tag="OO326_001535"
     CDS             16533..17282
                     /gene="moeB"
                     /locus_tag="OO326_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5965959.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17461..18123)
                     /gene="fsa"
                     /locus_tag="OO326_001536"
     CDS             complement(17461..18123)
                     /gene="fsa"
                     /locus_tag="OO326_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5965960.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18254..19153
                     /locus_tag="OO326_001537"
     CDS             18254..19153
                     /locus_tag="OO326_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5965961.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19159..21591
                     /locus_tag="OO326_001538"
     CDS             19159..21591
                     /locus_tag="OO326_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5965962.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21737..22552
                     /gene="ybiV"
                     /locus_tag="OO326_001539"
     CDS             21737..22552
                     /gene="ybiV"
                     /locus_tag="OO326_001539"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5965963.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22704..23969
                     /locus_tag="OO326_001540"
     CDS             22704..23969
                     /locus_tag="OO326_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5965964.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24210..25802)
                     /locus_tag="OO326_001541"
     CDS             complement(24210..25802)
                     /locus_tag="OO326_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5965965.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26021..26941
                     /gene="ldtB"
                     /locus_tag="OO326_001542"
     CDS             26021..26941
                     /gene="ldtB"
                     /locus_tag="OO326_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5965966.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26999..28117)
                     /locus_tag="OO326_001543"
     CDS             complement(26999..28117)
                     /locus_tag="OO326_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5965967.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28114..28581)
                     /gene="mntR"
                     /locus_tag="OO326_001544"
     CDS             complement(28114..28581)
                     /gene="mntR"
                     /locus_tag="OO326_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5965968.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28767..28895
                     /gene="mntS"
                     /locus_tag="OO326_001545"
     CDS             28767..28895
                     /gene="mntS"
                     /locus_tag="OO326_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5965969.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29167..30750
                     /gene="opgE"
                     /locus_tag="OO326_001546"
     CDS             29167..30750
                     /gene="opgE"
                     /locus_tag="OO326_001546"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5965970.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30798..31313)
                     /gene="ompX"
                     /locus_tag="OO326_001547"
     CDS             complement(30798..31313)
                     /gene="ompX"
                     /locus_tag="OO326_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5965971.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31366..31431)
                     /locus_tag="OO326_001548"
     CDS             complement(31366..31431)
                     /locus_tag="OO326_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5965972.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31666..32553
                     /gene="rhtA"
                     /locus_tag="OO326_001549"
     CDS             31666..32553
                     /gene="rhtA"
                     /locus_tag="OO326_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5965973.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32852..33355
                     /gene="dps"
                     /locus_tag="OO326_001550"
                     /gene_synonym="pexB"
     CDS             32852..33355
                     /gene="dps"
                     /locus_tag="OO326_001550"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5965974.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33759..34505
                     /gene="glnH"
                     /locus_tag="OO326_001551"
     CDS             33759..34505
                     /gene="glnH"
                     /locus_tag="OO326_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5965975.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34644..35303
                     /gene="glnP"
                     /locus_tag="OO326_001552"
     CDS             34644..35303
                     /gene="glnP"
                     /locus_tag="OO326_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5965976.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35300..36022
                     /gene="glnQ"
                     /locus_tag="OO326_001553"
     CDS             35300..36022
                     /gene="glnQ"
                     /locus_tag="OO326_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5965977.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36283..38507
                     /gene="ybiO"
                     /locus_tag="OO326_001554"
                     /pseudo
     CDS             36283..38507
                     /gene="ybiO"
                     /locus_tag="OO326_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38504..39430)
                     /gene="rlmF"
                     /locus_tag="OO326_001555"
     CDS             complement(38504..39430)
                     /gene="rlmF"
                     /locus_tag="OO326_001555"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5965978.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39706..39966
                     /locus_tag="OO326_001556"
     CDS             39706..39966
                     /locus_tag="OO326_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5965979.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40231..>40533
                     /locus_tag="OO326_001557"
     CDS             40231..>40533
                     /locus_tag="OO326_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5965980.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10093 a        10717 c         9864 g         9859 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcttatcggc cttaagcgcc tgcccaacca
      721 catagctggt catgatttta gtcaggctcg caggatccag tttctcatcc gcattgcctt
      781 ctgccagcac tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg
      841 gcgcttcaac ggtttgttcg gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag
      901 aacccgctgc aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat
      961 ccattcaaaa aaatacgtta tatcgccgta tgataagttc ggtgattaat aacgcacaca
     1021 caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt ttatgcaata tcctgccgct
     1081 tcgctgcttc tggtgtcatt tttttgccag ctgttgctta aaagttaact ttacttcacc
     1141 atcggagtcc tgttcagtct gtggctgcct gtcggaaaag gggtgagtat gattacgctg
     1201 tggggtcgga ataattcaac taacgtaaaa aaagtattgc tgacgctcga agaactggaa
     1261 ctaccttatg agcaaattct cgcgggccgt gagtttggga taaatcacga tgctgatttt
     1321 ctggcgatga accctaacgg gctggtgccg ttgttgcgtg acgacgaaag tgatctcatt
     1381 ctttgggaat caaacgccat tgtccgctat ctggcggcac agtacgggca aaaacgcctg
     1441 tggatcgact caccggcacg tcgtgcggaa gcggaaaaat ggatggactg ggcaaaccag
     1501 acgctcagta atgctcatcg cgggatcctg atgggattag tcagaacacc accggaagag
     1561 cgcgatcagg ccgccattga tgccagttgc aaagagtgcg acgccctgtt tgccctgctc
     1621 gatgcggaac tggcaaaagt aaaatggttc tctggcgacg agtttggtgt gggcgatatc
     1681 gctatcgcac cgttcatcta caatttgttt aacgttggcc tgacctggac accgcgtcca
     1741 aatctgcaac gctggtatca gcaactcact gaacgccccg cagtgcgcaa agtggtgatg
     1801 attcccgtta gctaattgcg tgggctaact ttaagtaatt ccccactgga ctcgtcggtg
     1861 agaacgtata aataaccgtc gggtccagtg cgaacatcac gaattcgctg ccctctgtcc
     1921 gttaaaatac ggccatcttc tgtcactttg tcgccgttga cgctcatcac aatgacatct
     1981 ttatctttca gctcccaata aataattttt gctgccactg ggggaattta tcgctgttat
     2041 agaaggccat gccgctcaca gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc
     2101 cggcgacgat ctcccctttc gcttccggta tcttaaagcc tgaatagttg attccccagg
     2161 ttgccagcgg ccagccgtag tttttgcctt tttgcgggat attaatttca tcaccaccgc
     2221 gcgggccatg ttcattcagc cacagtgcat tactccacgg attcatcgcc attccttgcg
     2281 gattacgaat gccataagac cagatctcgg cgcgcgcacc ggattccttt ataaaaggat
     2341 tatcatccgg gatttcgccc tggtcggtca gacgcaccag tttgccctgt aatttatcca
     2401 gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc aataaaaaga taacctttac
     2461 cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga cagttttggc atctggcgga
     2521 agacggtgcg gaagtcggtc acttttgaga gatcatcact taagcggcca taacccacag
     2581 cagttccggc tttgccatca tcgccaactt cggaataact taaccagatg cggcgagact
     2641 gagcaaaatc aggcgctaaa accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt
     2701 ccggaactcc ggaaagcggc gcagataatc cttttcctgc ttgccagtga cgcaactcgc
     2761 cgccgcgcag agtgattaac ataccgtgat tatcgggtaa aaaggccagt gcccagggat
     2821 ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc aggagccgcc cagagagcgg
     2881 aagaaagaca aataaggggc acaaggaaaa aggattgtcg atgcataacg cctccggatt
     2941 gactctggct taaagcgtag tcagtggagg agataacaaa ttcattttta caaaaactta
     3001 aacatgaagg gggagacgct ttctccccct tagttttcag gccttctcaa gcatggcgtg
     3061 cttctgcagg ctctggatac tcagcgttaa gctcatcaga caattttcaa gcttatcggc
     3121 gttgacggta ataacagtcg ggcaatcatg gtgcccactc atcaaacata ctgcggctgt
     3181 cgctaatgct tcttcagcat gatgaagagc actccactct tcctgatcca gatgaagatt
     3241 caaccgcagc gatttatcgt gcagttcgcg attcagttta aaaaagttat ctcgtagatg
     3301 attgctttcg ctgacggaca tgtatccttt tgccttcctc agttgcacgt atgcagcgag
     3361 gtcaacgcgt gcgtcgatca accggttcag cagatcgatt cgctgtctgt caacgaacat
     3421 acttcgatcc tcctctttcc agccttgttg caattaaata gtatggctat tttttgagca
     3481 aaatgctgtc cgggatcaat tcttcacgcc gaattaacaa aatttacatt tccttcgcct
     3541 ccgccagcga atgagattgc gatacaaatt ttatccagaa ctctgtagaa tccctgccct
     3601 gacctttcct gtaactgaat attttttaag ctatgagcaa agtaactccc cagccgaaaa
     3661 tcggctttgt ttcccttggc tgtccgaaaa accttgtcga ttcagagcgt attctcaccg
     3721 aactccgcac tgaaggttat gacgtggtac cgagctatga cgatgcgaac atggtgatcg
     3781 tcaacacctg cggctttatt gacagcgcgg tacaagaatc actggaagcc attggtgaag
     3841 cgttgaatga aaacggcaag gtaattgtga ccggttgtct gggggcaaaa gaagatcaga
     3901 tccgcgaagt ccacccgaaa gtgctggaaa tcaccgggcc tcatagctat gagcaggttc
     3961 tggagcacgt tcatcactac gtgccaaaac cgaaacacaa cccattcctg agcctggtgc
     4021 cagaacaagg tgtgaaactg acgccgcgtc attatgccta tctgaaaatt tcggaaggct
     4081 gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg cggcgacctg atgagccgtc
     4141 cgattggcga agtgttaagt gaagcgaaac gtctggtaga tgcgggcgtt aaagagattc
     4201 tggtgatctc gcaggatact tccgcctatg gcgttgatgt taaacatcgt actggcttcc
     4261 acaacggcga gccggtaaaa accagcatgg tcagcctgtg cgaacagtta tcgaaactgg
     4321 ggatctggac acgtctgcac tacgtttacc cttatccgca tgtggacgac gtcatcccac
     4381 tgatggcaga aggcaaaatc ctgccgtatc tggacattcc gttgcagcac gccagcccgc
     4441 gcattctcaa actgatgaag cgtccgggtt ctgtagatcg ccaactggcg cgcatcaaac
     4501 agtggcgcga aatctgcccg gaactgaccc tacgctcaac ctttattgtc ggcttccctg
     4561 gcgagacgga agaagatttc cagatgctac tcgacttcct gaaagaagca cgtctggatc
     4621 gcgttggctg ctttaaatac agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc
     4681 aggttccgga agaagtgaaa gaagaacgct ggaaccgttt catgcagctg cagcagcaga
     4741 tttccgccga gcgcctgcaa gagaaagtgg gccgtgaaat tctggtgatt atcgacgaag
     4801 tggacgaaga aggcgcgatt ggtcgcagca tggcagatgc accggaaatc gacggcgcgg
     4861 tttatctcaa tggtgaaacc aacgttaagc cgggtgatat cctgcgtgtg aaagtcgagc
     4921 acgccgatga gtacgatttg tggggtagcc gggtttaatt aattcaggcc gggcaaaatc
     4981 atttgtccgg cctgcgaata atgattactg ccagctaatc tgtgccctgc cgtttttctt
     5041 actcttatac agccgctcat ccgccagcat tttcaagcgt tcgacggtag tacattcatt
     5101 tgaatgcgct acaccgatac tggtgcttaa attaatgctt cccacctgga aatgaacgat
     5161 ttcttttagt atcgatagca actcttccag cgtgccgcca gtataaatca ccgcaaattc
     5221 gtcacctccg aagcggtaga ctttaccttt atctaccggg atctgtgatt taaggatttt
     5281 caccactttc atcaacactt catcgcccag caaatgaccg aaggtgtcgt taatgttttt
     5341 gaatttatcg acgtcaaaca gacacaaata accgcctttt tccggcagcg cttttaaatc
     5401 ttcatcaaag gattttctgc ccattgcttt ggtcaggtcg tccaggaacg ccgagttata
     5461 catactgtag agttttttga catcggcagc cgaaaagaaa agcccgccat ccagtacgcg
     5521 ttcaaaatgg ttattaatga tggcttcttt ctccacaata ccacgattaa tcactctgcg
     5581 taccagatag aggcaactgg cacccaatat cactgccacc aatgcggcaa agaaaataat
     5641 caatgtacaa acccgcaaat aaacatcttt tttatacgca aatacgtaca atccggtcgg
     5701 caaataggcg aagtgctcca tcgcttccga aacggtgaag atggcgtttt tagcattata
     5761 ttcatcaccg gtcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     5821 catcaccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5881 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5941 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     6001 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     6061 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     6121 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
     6181 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
     6241 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
     6301 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
     6361 cacttttatc ccaagcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
     6421 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
     6481 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
     6541 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
     6601 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
     6661 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
     6721 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
     6781 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
     6841 catccttcag cgacgcacgt tgactaaaat gtctgaatta acccattgcc gttttaataa
     6901 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
     6961 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg ctcagaatgg
     7021 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
     7081 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
     7141 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
     7201 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
     7261 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
     7321 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
     7381 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
     7441 tgcgttcatc aacaccaaac accgcctcca cggcttccag accacacagt ttgatgatcc
     7501 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
     7561 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
     7621 tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct tcgcgaattt
     7681 tcagagcctc gatctgatcc gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg
     7741 tcatttcaga aaattgccga cggaacacac gagtggtcat gaaatatacc accagcaaca
     7801 cgataataat gaacgcaaac ggtagataaa aaagtttatc tgatgcgttc aataaattac
     7861 cgccaggata ataaaaatag atttcgaaca atgcattatt tggcactgct gatgtctttg
     7921 caatatactc cgtatcgaca gaatcaataa tattataatt accttcgcta ttataaataa
     7981 tcagtttttc acgagcgagt tgattcttgg caatgatctc atgaccttgt ttcaaaaata
     8041 gaacttcatc attagcgctg atttgatcaa gatactcgtt cagataccgc cagtttaaat
     8101 cgacatgaat tttatctacc gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa
     8161 aagagataca ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt
     8221 tagagtcatc tgaagccacc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa
     8281 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
     8341 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
     8401 agtctaaaaa tggcatcatt tccattttca gaatcacggc gcgttgaatc tcctctgtat
     8461 tttttaataa atacagtgag tcctttaatg tcagggccga acgttcaata tctttaaaga
     8521 aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgtt
     8581 ccgataccag ttgatagtta atcatgaaaa aaagaccaat aaatgacagt gctgccaata
     8641 aaaataaaat tatcagcctt atcttttcgt ataaactcag catgttaata catttaacaa
     8701 aataactatc tgaacgctgt gccgctgcaa aaatagtccc ttgctgcggc tttttaacca
     8761 aattacccga ttctacaaaa tcagcagaaa gttacaacac ttgcttaggg caataatttc
     8821 ctgtgaggtg attacccttt caagcaatat tcaaacgtaa ctatccttta attttcggat
     8881 ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc cagtacggtc agaaaaatag
     8941 ccagggccgg aaaaacagcg acatgcggcg cgataaccat atccgctcgt gcctcattga
     9001 gcattgctcc ccactctggt gtcggcggct gcgcaccgag gccgagaaat gacaggctgg
     9061 cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa caccacgata gaagagacgg
     9121 tcccaggcag gatatgacgc aataaaatgg tcatatcgct ggcaccaata ctgcgtgctg
     9181 actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc gcgcaccagg cgggcaaacg
     9241 cggggatgga aaaaatggcg actgcgataa tcacgttagc aatgccgctt cccaacaccg
     9301 caacaacagc gatcgccagt aaaatacccg ggaaggcaaa cagcacatcg caaatgcgca
     9361 tgatcagccg atcccaccag ccttcataat atccagccaa caagcccagc aacgtcccta
     9421 tcgccacacc gataaacacg gcaaacacgc ccgccgccag cgagatttgc gcaccaacca
     9481 ggacacggct gaaaatgtcg cgccccagtg aatcgacgcc aaaccagtgc tgcaaagaag
     9541 gtccgttatt cagattgtca taatcaaaat aattttcggc gtcataggga gcgatccagc
     9601 gtgcaaaaat ggccaccaca atcaataaaa taacgaataa tgcggcggtc atcgccatat
     9661 gctgacggcg aaatcgtcgc cagaattcat gccacggtgt acgtacctgg tcaggtttga
     9721 ccagtggcat ggcgtttaac accgcctgac gtcgccagtt aaatagtcgc atccttactt
     9781 gtacctgata gccgggttaa tggcggcgta aagcacatcc accactaagt tgataagaat
     9841 aaattccagc gagaaaagca gaatttccgc ctgaatcacc gggtaatcgc gcatttctac
     9901 agagtcaacg agtaagcgtc caagtccggg ccagttgaaa actttctcca ccacgataga
     9961 accaccgagc aaaaagccaa actgtaagcc catcatggtc actaccggga tcatcgcgtt
    10021 gcgtagcccg tgtttgagga caacccaggt ttcgctcacc cctttcgccc tcgcggtacg
    10081 catataatct tcgcttaaaa cgtcgacaaa tgacgcacgg gtaaagcgcg ccatcacggc
    10141 ggccactgcc gcgccgagcg tcagggaggg taaaatgtag tgctgccaac tgtctgctcc
    10201 cacagtaggc agccagccca gttcaacgga gaatacctga attaaaaaca tccccagcgc
    10261 aaatgccgga aacgagatcc ccgacaccgc aatggtcata ctcaatcgat ccggccaacg
    10321 attacgccag acggcggcga taattcccgc cgccatacca aatatcaccg cccagaccat
    10381 actggttatg gtcagccata gcgttggcat aaagcggctg gcaatctcat cggcaaccgg
    10441 acgacgcgac accatcgaca ggccaaaatc cccctgcaca gcattgctga tatagtgcca
    10501 gaactggtga tacagcggct gatccaaccc cagctgctga cgcaccagtt ctataacctg
    10561 cgcatcagct tcgggcccgg caatcaatcg cgccggatcg ccgggcagca tgtggacaaa
    10621 taaaaacacc agcaccgaga cgataaacag cgtcggaatc aaccccagta atcgtttgat
    10681 aacgtaataa agcattccac tccctgcgtt gcttattgca aatccgcgtc ttcaaagctg
    10741 aagccggtat ctggcatgat ccaaaaaccg gtcaggtttt tactgtgtgc cgacaccagt
    10801 ttttctacca ccagcgggat ccacggcgat tcttgccaga tgatatcctg cgccgcctta
    10861 tataagcggg tcttttccgc cggatcattc gttttcagtg cctgagccag gaagtcatcc
    10921 acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg gtggccagtt ttgcgaggca
    10981 aacagcggcg atagtgccca gtcggcttcg ccggttgaag ccgaccagcc agtgtagaac
    11041 atccgcacgc cactctcttt ttgcccttta ccttcaactt ctgccgcccg ctgtccggca
    11101 tccatcgcgg tcacctgggc tttaatcccg acctgcgcta actgctgctg ggtaaattgc
    11161 agcactttct gcgcggtgct gtggttatgt gacgaccaca gcgtggtact gaaaccgttg
    11221 ggatatcccg cctctttcag taattcgcgc gctttcactg gatcgtaagg ccacggttta
    11281 taactttgcg cgtaggcgat acttggcggt accacaccag tagctggagt tgcatagccc
    11341 gcaaaggcaa ctttcaccag cgccggacgg ttaatggcgt aattcagcgc ctcacggacc
    11401 ttcgggttat cgaacggctt ttgcgtcacg ttcatactga tataacgctg cataattgac
    11461 ggactggcca tcaactcgat atttttgttt ttctccagca gtgtggcctg ctcgtaggga
    11521 atggggaaag caaactgcgc ttcaccggtt tgcagcattg ccgcgcgggt gttgttatcc
    11581 gccaccggac gccaggttat gctgtccagt ttgggcaatc ctggctgcca gtaacccgcg
    11641 aattttttca ccttcacaaa atcggtctga ttccaggtat ccagttcata cggtccggtt
    11701 cccaccggat gaaaaccaat ctccttgcca tatttttcca gcgctgtcgg tgaaatcatc
    11761 gcggtcgccg gatgggcaag aatattaata aacgctgaga acggctgttt gagggtaatc
    11821 tttaccgttg tcggatcgat cgcctccgtt ttggcaatat tcttatacag gttatagcgt
    11881 ttaagatgat tcgccggatc gctggcccgg tccagattcg cttttaccgc cactgcgttg
    11941 aaatcggtgc catcctggaa tttaattcct tcccgcaatt tcacggtgta agtaatgccg
    12001 tcatcggaaa cggtataact ctccgccagc acgtttttca gtttcatctc tttatccaga
    12061 ccgaacagcc cctggtaaaa cgatttcgct acggcctgag ataacgtgtc attcgcgtca
    12121 tacggatcga gcgtggtgaa attcgatcct accgccacca ccacatcttt ggcagcgaat
    12181 gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca ctaacccact acggtgtaca
    12241 gctcttgcca ttttattctc cgcctgaatg ttatctacgc atgaatgcgt attctgattg
    12301 tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac gagacggctg ccacctcttc
    12361 gccacgcaga tgaatattgc tgggaagatc gtccgacagc agtacacgct gcggtcgttg
    12421 tcgggacggt tcagcgaccg gaactgccgc cagtaattta cgcgtataag gatgctgcgg
    12481 gttttcgaag accgcgcacc gtgaaccaat ttcaacaatt tgcccgagat acatcaccgc
    12541 cacacgatga ctaatccgct ctaccacagc catatcgtgg gagataaaca gatacgcaat
    12601 gccgaaatca cgctggagat cgagcaacaa gttgataatc tgcccgcgaa tagaaacatc
    12661 cagcgccgaa acggcttcgt cggcaatgat cacttttgga ttcaatgcca acgcgcgagc
    12721 aatgcagatg cgctggcgct gaccgccgga aaactcatgc gggtaacgcc aggcatgttc
    12781 aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt gcagccgcgt ctttacctgg
    12841 caataaaccg tgtacacgca gcggttcgat aatcgaatca ccgatggtct gacgtgggtc
    12901 cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc cggcgcaatg cctgaagttt
    12961 gccaggtgac aaggtatcga ttcgctgacc gttaaagata atttcgccgc cctgcgattc
    13021 gaccaggcgc agcaacgccc gcccggtagt ggatttaccg ctgccagact cgcccaccag
    13081 cgatagcgtt tcgccaggcc agagatcaaa actgactttc tcaacggcat gcacttcccg
    13141 cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg gtgacaagat tacgcacccg
    13201 taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg gggggttcct gtttcgctgg
    13261 atgttcaagc gatatcaatg ggaaacgtcg gggataatct aaccctttca tcgcaccaag
    13321 ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt tgcggtgcat gaaaaatctg
    13381 ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc accagtaccc gatcggcaat
    13441 ctctgccacc acgcccatat cgtgagtgat aaagataacg cccatcgaca tctctttttg
    13501 caatactttg attaattgca ggatctgcgc ctgaatagtg acatccagcg cggtggttgg
    13561 ctcatcggca atcagcaccg ccgggcggca tgacagcgcc atcgcaatca tcactcgctg
    13621 gcgcatcccg ccagagagtt gatgcgaata acgtgaaaga atggtttgtg cctccggaat
    13681 gcgtacctga tccagcatcc gcttcgcctc gaccatcgct tcttcacgac tggcgttctg
    13741 atgcagacga attgattcgg caatctgttc acccacagta aatactgggt tcagcgatgt
    13801 catcggctcc tgaaatatca tcgccatatc cgcaccgcgc acatggcgca tttgtgcagc
    13861 gctctgctcg ctaagttcaa tcacatcacg actgcgccgc cgcaacagca ttttatcgca
    13921 ctgtactaaa ccgcccgcct gttccaacag acgcatcaat gccagcgcag tcactgactt
    13981 accggagccg gattcgccta caattgccag cgtctcaccg cgttgcagac taaaagagag
    14041 attgcggacc gcagctattt tctgctggtc ctgcataaag gcaatattaa gattttcaac
    14101 cgccagcaca ttaccggcat caagttcatc actgtgtggc aacggtgtcc cctttttcac
    14161 ggtagatacc ggtggttggc gtatcgcctg cgtagcccca ggcgcgatac attccttcgg
    14221 tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc gattaagcca ccgctaccgc
    14281 caagcgcagg gagtttttcc attactaccc gctcgcaggc ttccgcgaga cttaatccgc
    14341 cgtaatccat taacgcggcg atgtcatatg ccgccagcgc gcggatgaag acttcgcctg
    14401 tgccggtaca agaaaccgcc acactggcgt tattggcgta gcatccggca cccactaagg
    14461 gactatcgcc aactcgtccg ggtaatttat tggtcattcc gcctgtggac gtggctgccg
    14521 ccagattgcc gtctaaatcc aacgccacgg cccccacggt gcccattttc tgtttttcat
    14581 ccagtggcgc accgctatgg tcgaggactg ttgccccttc ctcgcgcgct gccagtagtt
    14641 gttcataacg caaaggcgtg gagaaaatct ccggtgagac gcgctccatg ccatgagcaa
    14701 acgcaaaatt ttctgcccct tcgccaatca tcatcacatg cgggctttgc tccatcacca
    14761 gccgggcggc aagaaccgga ttacgcagat gactaacgcc cgccaccgca ccggctttca
    14821 gggtgttacc atccatcaca caggcgtcca gttcatgggt ttcatcacgc gtaaagacag
    14881 cgccaattcc ggcgttaaac agtggacact cttccagcag acgcaccgct tccgtcacca
    14941 catccagcgc actttcgccg gcttccagca ttttctgccc ggtttcaaca atggcagaca
    15001 acgcctcgat gtagcgtaat tcctgttgca gactcatctg cgcgcggcta attgcacctg
    15061 cgccaccatg aattgcaatg actgctttgc ccatagtatt cgtccattaa gggtggctcg
    15121 ccacttttgc tataaatatc agaattttta tgaattactc attcaatttt tgaatataga
    15181 aagatggata tggcatgtaa aggcaggagc caacgctacg cccgaggtgg catacagtct
    15241 atggcgtttt tctgacataa taggcaaatt cgattttgcc tccgcaggag tgttttcatg
    15301 gaatttacca ccggattgat gtcgctcgac accgcgctta atgagatgct ttctcgcgtt
    15361 accccactga ccacccagga aacgctgcca ctggttcagt gttttggtcg tattctggcg
    15421 agcgatgtcg tttcgccact ggatgtcccg gggtttgata actccgctat ggacggctac
    15481 gcggtgcgtt tagccgatat tgcctccggg caaccgctgc ccgttgccgg taaatccttt
    15541 gccggtcagc cataccatgg tgaatggcct gcgggtacct gcattcgtat tatgaccggt
    15601 gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg agcagactga acaaacggac
    15661 aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa
    15721 gatatctctg caggtgcggt tgttttcccg gcgggaactc gcctgactac cgcagagctg
    15781 ccagtaattg cttcactggg gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg
    15841 cttttttcta ccggtgatga actccagttg cccggtcagc cgctgggcga cggccaaatc
    15901 tacgacacca accgtctcgc cgtacacctg atgttagaac agctgggttg cgaggtaatc
    15961 aacttaggga ttatccgcga cgatccacat gccctgcgcg ccgcatttat tgaagccgac
    16021 agccaggcgg atgtagtgat cagttccggc ggtgtttcag tgggtgaagc ggattacacc
    16081 aaaatgattc ttgaagagct gggggagatc gccttctgga agctggcgat taaaccaggt
    16141 aaaccgttcg cgttcggtaa actcagcaat agctggttct gcggcctgcc gggcaacccg
    16201 gtttcagcga cgctgacctt ctatcaactg gtacagcctt tgctggcaaa actaagcggc
    16261 aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa
    16321 acgccaggac gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt
    16381 gaagtgacga ccaccggaca tcagggttca catatattta gctcctttag tctcggcaac
    16441 tgctttatcg tgctggaacg cgatcgcggc aatgtggacg tgcgcgaatg ggtggaagta
    16501 gaaccgttta acgcgttgtt cggaggcctg taatggcgga actcagcgat caggagatgc
    16561 tgcgctacaa ccggcaaatc attctgcgcg gttttgattt tgacggtcag gaggcgctaa
    16621 aagattctcg tgtgctggta gtcggcctcg gtggactcgg ctgcgcagct tcgcagtatc
    16681 tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt cgacacggtt tcgctctcga
    16741 atctgcaacg ccagacactg cacagtgatg ccacggtcgg gcaaccgaag gtggaatccg
    16801 cccgtgacgc cctgacgcgg atcaacccac atatcgcgat tacgccagtc aatgcactgc
    16861 tagatgacgc agaacttgca gcattgattg ctgaacacga tctggtgctc gactgtacgg
    16921 ataacgttgc ggtacgtaat caactgaacg caggctgttt tgccgcgaag gtgccgctgg
    16981 tttccggcgc ggcaattcgt atggaagggc aaatcacggt cttcacttat caggacggcg
    17041 aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa cgcgttaacc tgcgtagaag
    17101 caggcgtgat ggcaccgttg ataggcgtaa ttggttcgtt gcaggcaatg gaagcgatca
    17161 aactgctggc aggttatggc aaaccggcca gcgggaaaat cgtcatgtac gatgcgatga
    17221 cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt
    17281 aattgccacc tccaagcctg ataagacgcg acaagcgtcg catcaggcat ttgctccaga
    17341 cgccagatgc ggcgtgaacg ccttatctgg cctacggttc gggcacagat ttccaagcgc
    17401 ggcaggcatc ggtgcactcc gccacgtcat cagcaattta tgaggatgca gaacgtccgg
    17461 ttaaatcgac gttctgccaa acgctccctg ccagtcctgc tcaaacttcg tcacagcggc
    17521 ttccaccgcc ggatagctaa tcatctgttg tgccacatcc agtggcagag taattgattc
    17581 acatccggcc agtaagcagt ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac
    17641 tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag tcggtcacag tctgaatgcc
    17701 gctaccgccc tgagcatcaa tacgattaac gtaaggcgca acatattccg cacctgccag
    17761 cgccgacagc agcccttgtg ccgcgccata taccgcggtt cccagcgtcg gaatcccttc
    17821 cgcttttaac atcttaatcg ctgccagccc ctcggctgtc accggaactt tcaccacgat
    17881 atccgcaata atagaacgca gcttaagcgc gtcattcacc atcccttcag cagtggtagc
    17941 cattacctgg gcaaacagac gcccctgacc gcccatcgct tcatgaagtt gcggaagcac
    18001 aacatccagc ggttttttac ccgcggcgat aatgcttggg ttagtggtca cacccgccag
    18061 cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct gaagtatcca gatacagttc
    18121 cataaccatc ctcaaaatgt actttttgtc tgaatgtaga ctatcacggg aaaactctct
    18181 gaacagttga cgcacatcaa tctaactttc attcgaaagt attttaatgt ttgaacgaaa
    18241 cagagaggca actatgattt tcaatattca gcgctactcg acccatgatg gccccggtat
    18301 ccgcacggtc gtatttctta aaggctgttc gctgggctgc cgctggtgtc agaacccgga
    18361 aagccgcgcc cgtacgcagg atctgctgta tgacgcacga ctgtgtctgg aaggctgcga
    18421 gctgtgcgct aaggccgcgc cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca
    18481 tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc gactgctgtc cgacacaggc
    18541 gttaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc atggcgaccg ttctgcgcga
    18601 taaaccgttt tacgatcgca gcggcggcgg cttaacgctt tcgggtggtg aacccttcat
    18661 gcagccggaa atggcgacgg cgctgctgca agcctgccac gaggcaggca ttcatactgc
    18721 ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc ccttctctgc cctatatcga
    18781 tctgtttctt gccgatttaa aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg
    18841 taacgccgcc agggtgctgg ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat
    18901 tatccgcgtg ccgctgattc agggctttaa tgccgacgaa acctctgtaa aagccattac
    18961 cgattttgcc gccgacgagc tgcacgttgg cgaaattcat tttctgccct accacacgct
    19021 gggcatcaac aaatatcact tacttaatct gccctatgac gccccggaaa aaccgcttga
    19081 tgcgccagag ctgctcgatt ttgcccagca gtatgcctgc cagaaagggt taaccgcgac
    19141 cttacgagga taacaaccat gaccacactg aaactggaca cgctcagcga ccgcattaaa
    19201 gcgcacaaaa atgcgctggt gcatattgtg aagccgccag tctgtaccga gcgcgcgcag
    19261 cactataccg agatgtatca acaacatctc gataagccga tcccggtacg tcgcgcgctg
    19321 gcactggcgc atcacctggc gaatcgcacc atctggatca aacacgatga gttgatcatt
    19381 ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg
    19441 atcgaaaaag agattgatga tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa
    19501 gagaacaaac gtgttctgca tgaagtgtgc ccgtggtggc gcggtcagac ggtacaggat
    19561 cgctgctatg gcatgtttac cgatgagcaa aaaggtctgc tggcgaccgg catcattaaa
    19621 gcggaaggca atatgacctc cggcgatgcg cacctggcgg tcaatttccc gctgctgctg
    19681 gaaaaagggc ttgatggtct gcgtgagaaa gtagcggaac gtcgctcgcg catcaatctg
    19741 acggtgctgg aagatttgca cggcgaacag ttcctgaaag cgattgatat catgctggtg
    19801 gcagtcagtg aacacattga acgtttcgct gccctggcgc gtgcaatggc cgcgaccgaa
    19861 acccgcgaaa gccgtcgcga tgaactgctg gcgatggcag aaaactgcga tcttatcgcc
    19921 caccagccgc cgcagacttt ctggcaggcg ctgcaactgt gttacttcat ccagttgatt
    19981 ttgcagattg aatctaacgg tcactcggtg tcgtttggtc gtatggatca gtatctctac
    20041 ccgtactatc gccgcgacgt tgaactgaac cagacgctgg atcgcgaaca cgccatcgaa
    20101 ttgctgcata gctgctggtt gaaattgctg gaagtgaaca aaatccgctc cggctcccac
    20161 tcaaaagcct ctgcgggaag tccgctgtat cagaacgtca ccattggcgg acaaaatctg
    20221 gttgatggtc aaccaatgga cgcggtgaat ccactctctt acgcgatcct cgaatcctgc
    20281 ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt accatgcagg aatgagtaac
    20341 gatttcctcg acgcctgcgt acatgtgatc cgctgcggct tcgggatgcc ggcattcaac
    20401 aacgacgaaa tcgtgatccc ggaatttatt aaactcggta ttgaaccgca ggacgcttat
    20461 gactacgcag cgattggttg tatagaaacc gccgtcggtg gcaaatgggg ctatcgctgt
    20521 accggcatga gctttatcaa cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt
    20581 gatgccacca gcggcaaagt gttcctgcca caagaaaaag cgttgtcggc aggtaacttc
    20641 aacaacttcg atgaagtgat ggacgcgtgg gatacgcaaa tccgttacta cacgcgtaaa
    20701 tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag agaacgtgca cgatattctc
    20761 tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg
    20821 aaatatgact gggtttctgg cctgcaggtc ggtattgcca acctcggcaa cagcctggcg
    20881 gcagtgaaga aactggtgtt tgaacagggc gcgattggtc agcaacagct ggctgccgca
    20941 ctggcggatg acttcgatgg cctgactcat gagcaactgc gtcagcggct gattaacggt
    21001 gcgccgaagt acggcaacga cgatgatacc gtcgatacgc tgctggctcg cgcttatcag
    21061 acctatatcg acgaactgaa acagtaccat aacccacgct acggtcgtgg tccggttggc
    21121 ggcaactatt acgcgggtac gtcgtctatc tccgctaacg taccgtttgg cgcgcaaact
    21181 atggcaacgc cggacgggcg taaagcccac accccgctgg cagaaggcgc tagtccggcc
    21241 tctggtactg accatctcgg ccctactgcg gtcattggct cagtgggtaa actgcctacg
    21301 gcagcgattc tcggcggcgt gttgctcaac cagaaactga atccggcaac gctggagaac
    21361 gaatctgaca agcagaaact gatgatcctg ctgcgtacct tctttgaagt gcataaaggc
    21421 tggcatattc agtacaacat cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg
    21481 gatcagtacc gcgatctggt agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc
    21541 tctccagacg ctcaggacga tatcatcgcc cgtactgaac atatgctgta atctcttcgg
    21601 cccggctgcg gcgtcgccgg gctaaatcat tcacatcatc aatttcatcc ttactttcat
    21661 tcgaaatata atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg
    21721 agccaggagc acacctatga gcgtaaaagt tatcgtcaca gatatggacg gtacttttct
    21781 taacgacgcc aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa
    21841 gcgcggcatt aagttcgttg tcgccagcgg taatcagtat taccagctta tttcgttctt
    21901 tcctgagcta aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca
    21961 tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga
    22021 gttgctaaaa gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag
    22081 cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa cactaccatc gcctgaaacc
    22141 tgtaaaagat tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccgga
    22201 tgaacaaatc ccgttagtga tcgacaaact acacatagcg ctggatggca ttatgaaacc
    22261 cgtcaccagt ggttttggct ttatcgacct gattattccc ggtctacata aagcaaacgg
    22321 tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga
    22381 cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc
    22441 tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc
    22501 gctgaatgtg attcaggctg tgctggataa cacatcccct tttaacagct gacctctcta
    22561 ccggagccat gcgctccggt tttcccttct ttttacatcc tgtacatcac atcaaatcat
    22621 taaacttgca ttaacttatt tataacttgt attgtcacat gcaaattagc atagtttcga
    22681 atgaaagtta atgcaaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg
    22741 atcacaaagc agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgatgtcc
    22801 agcagatctt taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac
    22861 tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta tgccgatatc aaagcaggtc
    22921 atgttactgc agagcagcgc gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc
    22981 atttcccccg cgaacaagcg ctaggctggg atcagtcgat gctggactat ctgaaccgca
    23041 accgctttga cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt
    23101 cacgtcccga gatttacccc atctactggt cgcaggcgca aatgcaggcc cgccagagtg
    23161 aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa
    23221 agcaatggtt taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccttccgc
    23281 ccggaacgac ctccaaaggt cttggagcgc ataccgactc cggggcgctg gaacgctggc
    23341 tgcttccagc gtatcagcac gttttcgcca acgtctttaa tggcaatctg gcgaaatacg
    23401 atccctggca tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca
    23461 aatgttccgt gtttcggaca ttccagggct ggactgcgct ctcggatatg ctgcctggtc
    23521 aggggttgct gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc
    23581 tgcttgatga tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg
    23641 tatcagagca atggcatcca ctgttaattg aggcgttaac cagcattcca aaactcgagg
    23701 ccggagactc cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc
    23761 aacagggttg gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg
    23821 cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc
    23881 gcgaggacta tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg
    23941 gtaagcgagc gttgggcatg gatgtttgag agttaatgga agtgaattga tgtgggatta
    24001 agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc
    24061 ggcgttaacg ccttatccgg cctacaaagt cctgcagatt caatatatta caggtactgc
    24121 gtaggcctga taagcgtagc gcatcaggct attttgcatt tgtcatcagc cgtcgtcacc
    24181 ccgacagtat cggggtgacg gataatcact tactcgatcc ctttactacg caggtaatct
    24241 tcgtaattac cgctaaagtc gatcacgcgt tccggggtga tttccagaat gcgggtcgcc
    24301 agggagctta cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc
    24361 agcgccatgt tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg
    24421 atcagaatgt tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca
    24481 ccggaaagca ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga
    24541 atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac
    24601 acggtcagat cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg
    24661 ttttcagacc attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc
    24721 agcgttgatt taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc
    24781 agcaggttga gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact
    24841 tccagcgcgt tacggaacag tttcttatcc tgttcaaaac ggataaacgg gttctgacgg
    24901 ctggaggctt tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc
    24961 tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc
    25021 tgcgctttct tcttggcgtt atcggccagc agacgttcac gcgcttgggt cgccgccgtc
    25081 atgtactcat cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg
    25141 tgggtacaga ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca
    25201 cgctcgttca gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt
    25261 tcgtcgagca ggagaatatc cggctcagca aacagcgcct gcgccagaag cacacgcagc
    25321 ttccagccag gagcaacttc actcatcggg ccgtagtgct gttccactgg aattcccacg
    25381 ccaagcaaca gttcaccggc gcgagcttcc gcagagtaac cgtccatttc gccgtatttc
    25441 acttccagat cggccacttt atagccgtct tcttcactca tttccggcaa agcatagatg
    25501 cggtcgcgct cctgcttcac ttcccacaac tctttatgcc ccatgatcac cgtatccagc
    25561 acagtgaact cttcaaaggc aaactgatcc tgacgcagct taccaatgcg ctcgttggga
    25621 tcgagggaaa cgttacccag cgtcggctca aggtcgccgc cgagaatctt cataaaggtg
    25681 gatttaccac taccgttcgc gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg
    25741 gaaatgtttt caaacaacgg cttactgccg aactgcatgg tgacgttact ggaaactaac
    25801 acaggcgtat cctgaaaaga gatatgacaa accgcgcatt atgccataag tctgaaagaa
    25861 gatcctgctc ctcgcttaag gtctacactc aagccaaagg caaaaaatgt gatttcgtac
    25921 acatctgatt tcactgtgag ctggaatgaa cttataatgc gcttccaata ctctaatatt
    25981 ctcaacccaa tggcctgcca ggcacaaaat ctcgcttaac atgaatatga aattgaaaac
    26041 attattcgca gcggccttcg ctgttgtcgg cttttgcagt accgcctctg cggtaactta
    26101 tcctctgcca accgacggga gtcgcctggt tggtcagaat caggtgatca ccattcctga
    26161 aggtaacact cagccgctgg agtattttgc cgcggagtac cagatggggc tttccaatat
    26221 gatggaagcg aacccgggtg tggatacctt cctgccgaaa ggcggtactg tactgaacat
    26281 tccgcagcag ctgatcctgc cggataccgt tcatgaaggc atcgtcatta acagtgcaga
    26341 gatgcgtctg tattactatc cgaaagggac caacaccgtt atcgtgctgc cgatcggcat
    26401 tggtcagtta ggcaaagata cgcctatcaa ctggaccacc aaagttgagc ataagaaagc
    26461 aggcccgacc tggacgccga ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc
    26521 gctaccggct gtcgttccgg caggtccgga taacccgatg ggtctgtatg cactctacat
    26581 cggtcgcctg tatgctatcc atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag
    26641 tcatggttgt gtgcgtctgc gtaacgaaga catcaaattc ctgttcgaga aagtaccggt
    26701 cggtacccgc gtacagttta ttgatgagcc ggtaaaagcg accaccgagc cagacggcag
    26761 ccgttatatt gaagtccata acccgctgtc taccaccgaa gcccagtttg aaggtcagga
    26821 aatcgtgcca attaccctga cgaagagcgt gcagacagtg accggtcagc cagatgttga
    26881 ccaggttgtt cttgatgaag cgatcaaaaa tcgttccggg atgccggttc gcctgaatta
    26941 atcgtcaaaa cgtaaaacaa aggcggactc ataatccgcc ttttttattt gccagacctt
    27001 accaggccgg gatcataact aacaaaacgt atcccaccag cgccgcccac aacagcatcg
    27061 gtattgaata gagatggaaa cgccaccaga tgcggcgatc gttcgccata cgtagcgcaa
    27121 tcaaatttgc cagcgatcct ggtaataaac caaagccccc cacgtttacc gcccatgcca
    27181 gtaataaaga cggaggtaca taattgagca gcaaaatggt actcggcacg ttactgatca
    27241 cctgcgataa accgactgcc gttaaccata acccgggttc agataaatga ctcacgttac
    27301 ccaacacgcc ttgcaacgct ggaagctggg tcagtaaatg gacgtcgata aacatcgcca
    27361 taaacaccag cagcaacgtc cagtccacac tcaacaccac gcgacgcgcc agcagcacaa
    27421 agcccgccgc cacaatcacc agtccccaca gctcttgttt gaactccagc gccgtcagaa
    27481 agacgatata cagccccaga caattccaca ccagccgcgg tttccactcc ggtgtttgca
    27541 cccccgtatg gtattgcagc gcctttccag ggaaacaaca ccagcacaac agcaggagtg
    27601 tcagcatcat tgcgccagcc atcggtgcca tttgggcaat aaatccggca aacgaaagac
    27661 cagaacgtcc ccagataaga atattttgcg ggttgccaat tggcgtcagt agcgaaccag
    27721 cgttgactgc cagcgcctca aaaataatca gccgattaac cgggatctca cacagtcttt
    27781 ttagcgtgat agtcagcgga acaacaataa acagcgcgac atcgttggtc agaaaggtag
    27841 aaagcagcgc cgccgccagc accataaaca tcgccagccg acgctccgta gcaaagcggc
    27901 gcaccatttt gcgccccagc acatcaaaat aaccgcttaa ctccacacct ttggtcagca
    27961 gcatcaggcc gcttaaggtg atgatggtgt gccagtcgat agcagcaggc caggatttcg
    28021 gtgcaaaggg cacgaaaaag cttaatccga taccaacaag aattaataac tgaaaaaaac
    28081 gatcgccttg cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa
    28141 caaacgaaaa gcgtcgagcg tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg
    28201 acgggcgatt tccggactga cgcccaacac caacaagaaa ttttcgacta tctgatgtcg
    28261 ctcgcggctt tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg
    28321 gatcatttca atcagcccca tggttgccag ccgcttaagc attttagcca ccgtcggttg
    28381 cgaaactccc agacgagcag ccatgtccac ctgacgagct tcccccactt ccctgatcaa
    28441 gtcagaaatc agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg
    28501 gcggaaccct tcaacgtgct cttccacgtt cactaactgc gtcacttttt ttgctattgg
    28561 cgtacctgcg cgacgactca ttgtgcttcc tcattatggt gacgcataca tagcgttctc
    28621 atttcgtgcg cacattgtaa accagagttg cgaaggtaca aaaaattaac gttttagcaa
    28681 tagctatata atatagcctg tgctatatct gtatgtaatg caatcatccc tcaaggatcg
    28741 acgggattag caagtcagga ggtcttatga atgagttcaa gaggtgtatg cgcgtgttta
    28801 gtcattctcc ctttaaagta cggttaatgc tgctctctat gttgtgcgat atggtcaaca
    28861 acaaaccgca gcaagataaa ccttcagata aatagcggcg tcgcggtacg ccgcttcact
    28921 cctgctttta tgcaggcata acgcgttttg gtctgaaaaa aaccactttt tgtcggattt
    28981 gcaatcccct tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg gctcgctcta
    29041 tcttcttgca gccctgcgta tattgcggct cgcggatgcg gacccctttc ccctcttcac
    29101 gcactcttgc aggtattgac ccttgacgcc agggtaagca catggcgttt gttacgatag
    29161 tggcatatga atttaaccct caaagaatcg cttgttaccc gtagccgggt atttagcccg
    29221 tggactgcgt tctacttttt acagtcgcta ttaattaacc tcggcttagg ttaccccttc
    29281 agtttgctct acaccgctgc gtttacggct attttgcttt tgctatggcg aacattgcct
    29341 cgcgtacaaa aagttctggt cggtgtcagt tcgctggtgg cggcttgtta tttccctttt
    29401 gctcaggcct acggcgcgcc taatttcaat acattgctgg cattgcactc caccaatatg
    29461 gaagagtcga ccgaaatcct gacgattttt ccgtggtaca gctacctggt cggcttattt
    29521 atttttgcgc tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg
    29581 aataccttcg acagcctgtg tctggtattc agtgtggcga cattttttgt tgctcccgtg
    29641 caaaacctgg cctggggtgg cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt
    29701 tttgctaagg atgtcatcgt caataataac gaggtgattg aagagcaaga acggatggca
    29761 aaactttccg gaatgaaaga tacctggacg gtcactgccg ttaagccgaa gtatcagacc
    29821 tatgtggtag tgatcggtga aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac
    29881 tgggacaata ccccgtttgc cagcagcgtt aacggtttga tatttgctga ctacattgct
    29941 gccagtggct ccacgcagaa atcgcttggc ttaacgctca atcgcgtagt cgatggcaaa
    30001 ccacagtttc aggataactt tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg
    30061 ttttccaacc agggccaaat cggcgaatac gatactgcta tcgccagcat cgccaaacgt
    30121 gcagatgaag tgtacttcct gaaagaaggt aattttgaag cagataaaaa cacgaaagac
    30181 gaagcgttac tggatatgac cgctcaagtg ctggcgcaag agcactcgca accgcagctg
    30241 attgttctgc atctgatggg ctcgcatccg caggcctgcg acaggacaca aggcaaatac
    30301 gaaacctttg tgcaatcgaa agaaacgtcg tgctatctct ataccatgac gcaaacggac
    30361 gatttactgc gcaagctgta cgatcagtta cgcaacagcg gcagcagctt ctctctggtt
    30421 tacttttctg accacggtct ggcctttaaa gagcgcggca aagacgtgca ataccttgcc
    30481 catgatgata aatatcagca aaatttccag gtgcctttta tggtcatttc cagcggcgat
    30541 aaagcgcatc gggtgattaa agcccgccgc tcagccaatg acttcttagg ctttttctcg
    30601 cagtggacgg gaattaaagc gaaggaaata aatatcaaat acccgtttat atctgagaag
    30661 aaagccgggc cgatatacat caccaacttc cagttacaga aggtggatta caaccatctc
    30721 ggaaccgata ttttcgaccc gaaaccttaa aaacaaaaat ccgccccgag aggcggattt
    30781 ttatatcacc aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca
    30841 acgctacgaa tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg
    30901 aactgcagac cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg
    30961 tattcagtgg tctggaattt accataaccc acacccacta caccgtagat gcttgcccag
    31021 tcgttaatgc ggtaagccgg accagcagtg atgccgtagt actggttttt gttgtagtca
    31081 ccagagcttg cagtacggct tttctcggtg taagtgaaag aaccgatcac acccagcggg
    31141 ctgttgtctt cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc
    31201 tgagcgtcgc tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct
    31261 gcggtgaaag ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc
    31321 tcaaatgtga ttcaaataag tcctaagttt taaatatatc aaaaattaat gggaaactct
    31381 tcgcgatttg tgatgtctaa cgggccattt catgtaacag aacgtttcca tacaccgcta
    31441 tccatctaaa tttaaatcac tttttcagag aactgcgtaa gtattacgca tgttttccct
    31501 gtcattcatc cagattattc ctaatcacca gactaatgat tccatcaatc ctggcgcatt
    31561 ttagtcaaaa cgggggaaaa ttttttcaac aaatgctcaa ccagcattgg gtatatccag
    31621 tacactccac gctttactta agtctagata tttgtgggag aaaggatgcc tggttcatta
    31681 cgtaaaatgc cggtctggtt accaatagtc atattgctcg ttgccatggc gtctattcag
    31741 ggtggagcct cgttagctaa gtcacttttt cctctggtga gcgcaccggg tgtcactgcg
    31801 ctgcgtctgg cattaggaac gctgatcctc atcgcgttct ttaagccatg gcgactgcgc
    31861 tttgccaaag agcaacggtt accgctgttg ttttacggcg tttcgctggg tgggatgaat
    31921 tatctttttt atctttctat tcagacagta ccgctgggta ttgcggtggc gctggagttc
    31981 accggaccac tggcggtggc gctgttctct tctcgtcgcc cggtagattt cgtctgggtt
    32041 gtgctggcgg ttcttggtct gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc
    32101 gatttaaccg gctgtgcgct ggcactgggg gccggggctt gttgggctat ttacatttta
    32161 agtgggcaac gcgcaggagc ggaacatggc cctgcgacgg tagcaattgg ttcgttgatt
    32221 gcagcgttaa ttttcgtgcc aattggagcg cttcaggctg gtgaagcact ctggcactgg
    32281 tcggttattc cattgggtct ggctgtcgtt attctctcga ccgctctgcc ttattcgctg
    32341 gaaatgattg ccctcacccg tttgccaaca cggacatttg gtacgctgat gagcatggaa
    32401 ccggcgctgg ctgccgtttc cgggatgatt ttcctcggag aaacactgac acccatacag
    32461 ctgctggcgc tcggcgctat catcgccgct tcaatggggt ctacgctgac agtacgcaaa
    32521 gagagcaaaa taaaagaatt agacattaat taaatttaca tttctgtatg gttatgcata
    32581 accatgcaga atttctcgct acttttcctc tacaccgtat ttatatatcg aattatgcaa
    32641 aagcatattt attccgaaaa ttcctggcga gcagataaat aagaattgtt cttatcaata
    32701 tatctaactc attgaatctt tattagtttt gtttttcatg cttgttacca ctattagtgt
    32761 gataggaaca gccagaatag cggaacacat agccggtgct atacttagtc tcgttaatta
    32821 ctgggacata acatcaagag gatatgaaat tatgagtacc gctaaattag ttaaatcaaa
    32881 agcgaccaat ctgctttata cccgcaacga tgtctccgac agcgagaaaa aagcaacagt
    32941 agagttgctg aatcgccagg ttatccagtt tattgatctt tctttgatta ccaaacaagc
    33001 gcactggaac atgcgcggcg ctaacttcat tgccgtacat gaaatgctgg atggcttccg
    33061 caccgcactg atcgatcatc tggataccat ggcagaacgt gccgtgcagc tgggcggtgt
    33121 agctctgggg accactcaag ttatcaacag caaaaccccg ctgaaaagtt acccgctgga
    33181 catccacaac gttcaggatc acctgaaaga actggctgac cgttacgcaa tcgtcgctaa
    33241 tgacgtacgc aaagcgattg gcgaagcgaa agatgacgac accgcagata tcctgaccgc
    33301 cgcgtctcgc gacctggata aattcctgtg gtttatcgag tctaacatcg aataaatcca
    33361 tcgctgatgg tgcagaactt tagtacccga taaaagcggc ttcctgacag gaggccgttt
    33421 tgttttgcag cccacctcaa cgcacttatt tagtgcatcc atctgctatc cccagctgat
    33481 taagtaaatt ttttgtatcc acatcatcac acaatcgtta cataaagatt gttttttcat
    33541 caggttttac gctaaataat cactgtgttg agtgcacaat tttagcgcac cagattggtg
    33601 ccccagaatg gtgcatcttc agggtattgc cctataaatc gtgcatcacg tttttgccgc
    33661 atctcgaaaa atcaatgagt tgcaaaattg gcacgatttt ttcatatatg tgaatgtcac
    33721 gcaggggatc gtcccgtgga tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt
    33781 cactggctgc actgaccctg gcttttgcgg tttcttctca tgccgcggat aaaaaattag
    33841 ttgtcgcgac ggataccgcc ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg
    33901 gctttgacgt tgatctgtgg gctgccatcg ctaaagagct gaagctggat tacgaactga
    33961 agccgatgga tttcagtggg atcattccgg cactgcaaac caaaaacgtc gatctggcgc
    34021 tggcgggcat taccatcacc gacgagcgta aaaaagcgat cgatttctct gacggctact
    34081 acaaaagcgg cctgttagtg atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag
    34141 atctcgacgg gaaagtggtt gctgtgaaga gcggtactgg ctccgttgat tacgcgaaag
    34201 caaacatcaa aactaaagat ctgcgtcagt tcccgaacat cgataacgcc tatatggaac
    34261 tgggcaccaa ccgcgcagac gccgttctgc acgatacgcc aaacattctg tacttcatca
    34321 aaaccgccgg taacggtcag ttcaaagcgg taggtgactc tctggaagcg cagcaatacg
    34381 gtattgcgtt cccgaaaggt agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa
    34441 ccctgcgcga gaacggaact tacaacgaaa tctacaaaaa atggttcagt actgaaccga
    34501 aataataacg ctacacctgt aaagcgcact ggcagttccc tctcccccct ggggagagga
    34561 ttagggtgag gggcgcaaac ccgctccggg gccattaatt accctgaatt tgattattta
    34621 caccacggta acaggaacaa catatgcagt ttgactggag tgccatctgg cctgccattc
    34681 cgcttctgat tgaaggtgcc aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg
    34741 gtctggtaat cggattgctg gcaggttttg cacgcacctt cggtggttgg atagccaacc
    34801 acgtcgcgct ggtctttatt gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt
    34861 ttatctattt cgccctgccg atggcgttta acgacttacg catcgaccca tttactgcgg
    34921 cggtggtcac catcatgatc aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg
    34981 tgctgtctat ccacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg
    35041 aaaccattcg ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta
    35101 accagtggat catcagcatt aaagacacct cgctgtttat tgttatcggc gtggcggaac
    35161 tgacccgtca ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg
    35221 ccgtggcggt gttctatctg attattaccc tggtgctgag ctttattctg cgtcgtctgg
    35281 aaagaaggat gaaaatcctg tgattgaatt taaaaacgtc tccaagcact ttggcccaac
    35341 ccaggtgctg cacaatatcg atttgaacat tgcccagggc gaagtcgtgg tgattatcgg
    35401 gccgtccggt tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac
    35461 ctccggcgat ctgattgtcg atggcctgaa ggtcaacgat ccgaaagttg acgagcgcct
    35521 gattcgccag gaagcaggta tggtgttcca gcagttttac ctcttcccgc atctgacggc
    35581 gctgggaaac gtcatgtttg gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga
    35641 aaaactggcg cgtgagctgc tggcgaaagt cggtctggca gaacgtgcac atcactaccc
    35701 ttccgaactt tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa
    35761 gccgaaaatg atgctgtttg atgaaccgac ttccgctctt gacccggaac tgcgccatga
    35821 agtgctgaag gttatgcagg atctggctga agaagggatg acgatggtga tcgtgaccca
    35881 cgaaatcggt tttgccgaga aagtagcttc gcggctgatc tttatcgaca aaggccggat
    35941 tgcggaagat ggcaatccgc aggtgttgat caagaacccg ccgagccagc gcttgcagga
    36001 atttttgcag cacgtctctt aataagacac attgcctgat gcgctacgct tatcaggcct
    36061 acaggatatc tggcaactta ttaaaattgc atgaacttgt aggacggata aggcgttcac
    36121 gccgcatccg gcaaaaaagc ccgcacgttg tcagcaacct gcttaatatc ccttcctccc
    36181 tttcacccga aagggaggca caccagattc ctctcattta aaatcgcccc tcccccagca
    36241 tctatactta tctttttgct ctgttttctc actggaggag tcatgcggtg gatcctgttc
    36301 atcctcttct gcctgctggg cgcacctgcc cacgcggtat ccatacccgg cgttacaacc
    36361 acaacgacaa cggactcaac gactgaaccg gccccggaac cggatatcga acaaaaaaag
    36421 cggcctatgg cgcactggcg gatgtgctgg ataacgacac ctcgcgtaaa gagttgatcg
    36481 accagttgcg tactgtcgcc gcgacgcccc ctgcggaacc ggtcccgaag atcgtgccgc
    36541 cgacgctggt tgaagagcaa accgtgctgc aaaaggtcac cgaagtcagc cgccattatg
    36601 gtgaagccct ttccgcccgc ttcgggcaac tttatcgcaa tatcaccggc tccccgcata
    36661 agccatttaa tccacaaacc ttcagcaatg cgctgagcca tttttcaatg ttagcggtat
    36721 tagtgtttgg tttttactgg ctgattcgcc tgtgcgcact gccgctatat cgcaaaatgg
    36781 gtcagtgggc gaggcaaaaa aatcgtgagc gcagcaactg gttgcagctt ccggcgatga
    36841 ttattggggc gtttattatc gacctgctgt tactggccct gacattattt gtcggccagg
    36901 tattaagcga caacctgaat gcaggcagtc gcaccatcgc tttccaacaa agtttgtttc
    36961 tcaacgcctt tgccctgatt gaatttttca aagccgtact acgcctgatt ttttgcccaa
    37021 acgtggcgga gctgcgcccg ttcacgattc aggacgagag cgcccgttac tggggtcgtc
    37081 gcctgagctg gttaagcagc ctgattggct atggcctgat tgtggccgtg ccgattatct
    37141 ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg ctgtgcatga
    37201 ccgtctgggc gttgtacctg atcttccgaa ataaaaaaga gattacccag catatgctca
    37261 actttgcgga gcattcgctg gcttttttca gcctgtttat ccgcgccttt gcgctggtgt
    37321 ggcactggct ggcaagcgcc tattttatca tgctgttttt cttttcgttg ttcgatccgg
    37381 gcaacagtct gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt attggtatcg
    37441 cagcgtttgt ttccggtatg ttttcccgct ggctggcgaa aaccatcacc ctctcgccac
    37501 atactcagcg taactatccg gagctacaaa aacggctgaa tggctggctt tcggcggcgc
    37561 tgaaaacggc gcgtattctg accgtctgcg tggcagtaat gttgctgttg agcgcatggg
    37621 gattgttcga tttctggaac tggctgcaaa acggcgcggg gcagaaaacc gtagatatcc
    37681 tgatccgcat cgcactcatt cttttcttct cggcggttgg ctggacggtg ctcgccagtt
    37741 tgatcgaaaa ccggctggct tcggatattc atggccgccc gctacccagc gcccgtacgc
    37801 gtaccctgct gacgctgttc cgtaacgcgc tggcggtgat tatcagtacc atcaccatca
    37861 tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt gccggggcat
    37921 taggtctggc tatctcgttt ggttcgcaaa cgctggtaaa agatattatc accggggtat
    37981 ttattcagtt tgaaaacggc atgaacactg gagatttggt gactatcggg ccgttgaccg
    38041 gcacagtgga acggatgtcg attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc
    38101 acatcattcc gtggtcttcg ataaccacct ttgctaactt cgtccgcggt attggttcgg
    38161 tagtggcaaa ctacgatgtt gatcgccatg aagatgctga taaagccaat caggcactga
    38221 aagatgcggt aacggaattg atggaaaacg aagaaattcg cgggctgatt attggtgaac
    38281 cgaactttgc cgggattgtc ggcttaagca ataccgcgtt tacactgcgt gtttcgttca
    38341 ccacgctgcc actcaaacag tggacggtac gctttgccct cgacagccag gtgaaaaaac
    38401 atttcgacct ggcgggcgtt cgcgcgccag tgcagactta tcaggtgctg cctgctccgg
    38461 gcgcgacccc ggctgaaccg ctgccgccgg gggagccaac gctttaacgt tggcgattaa
    38521 caaaacggcg gcgctgctcg tcgttcataa aggtccaggc aataaagcga ctctgctttt
    38581 gcccctgggc catctctttt tttaccacct tcaccgcgcc cacgtcggtc agagcacgat
    38641 acaacggcgg taagttttca ccacgagata ccagcgaggt aaaccacatc acctgcttcg
    38701 cgaagccttt gctctcttca atcatctttt tgataaagtt gacttcaccg ccttcacacc
    38761 acaactcctg ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc cccaggttac
    38821 ggcgtttacg ctcactacct gcccgcgccg cagcggcgga atcgtggaat ggcgggttac
    38881 acaaggtcgc gtcgtattgc tcgtttttgt ggatgatgcc gttaaaaatc gccccactct
    38941 ctttttgccg acgcagacga atggcgcggt taagccccgg attagcactg ataatcgcct
    39001 gcgcactgct taacgcctgg ctgctggttt cactaccggt aaaacgccag ccatattcat
    39061 gtacgccaat cagcggataa atacagttcg caccaacgcc gatatccaga atgctggcat
    39121 tcgctggaat tgttccgctt gcttcagcca gtaaatcggc aaggtgatga atataatccg
    39181 cccggcccgg tactggtggg cagagaaaac cgtcggggat atcccagttc gctacggcgt
    39241 aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg gcaaagtcta
    39301 cgctttgctc ccctgcgggt gtaagcgtga ggaattgcct gagttcagga ttgacctgac
    39361 aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac cccggtttct
    39421 gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa cgcgacaggg
    39481 taaatatctg gctgcgttta tcattcccca aaaccttaac atggctatgg tttatggcaa
    39541 ttcatcataa aaatcagcat tatgcatgtc attgatttgt cttattttgt aaaaatatat
    39601 cgttcaaaaa ataagcattt ctctgtcaat tttttgcgtc atttgcatcg cgcaggcgac
    39661 ctacacttat caggcactac ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc
    39721 acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc cgcgcaaccg
    39781 atgagtaacc tggacagcgg tcagctacgg cccgccggta ccgtttcggc aactggcgca
    39841 tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgaaca aggcgcgaaa
    39901 ggttatgtca ttaattccgc aggcggaaat gaccagatgt taggtactgc aaccatctac
    39961 aaataacccc accaacgtaa ctgcattcgc ctctggatgc agttttcgcc tgttttgtaa
    40021 tcaaaaaaca tcaaatatga acgcaatgta aataaatgta tttctttttc gcgcaatggg
    40081 tgatagaaaa tcgcatcaag tgataatgct tatcaaaatt attatcactt gcacgaacac
    40141 tatcacggga ttaacagtgg catcgcatcc gcagagatgc tttctcgtgg cagtgaaaat
    40201 ttcaacatat aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt ccctgccaga
    40261 caatttcatt cgctcacgtt ctttgccggt ctttgtattg gtattacgcc tgtggcacag
    40321 gcactcgccg ccgaagggca agctaacgcg gatgacacgc tggttgtcga agcatcaacg
    40381 ccttcgcttt atgcgccaca acaatctgcc gatccgaaat tctcgcgtcc ggtagcggat
    40441 actacccgca cgatgacggt gatttctgaa caagtgatta aagatcaggg cgcaaccaac
    40501 cttaccgacg cgctcaaaaa cgtccccggc gtg
//
LOCUS       DAJTRW010000027        39934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRW010000027 DAJTRW010000000
VERSION     DAJTRW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            309..899
                     /gene="uidR"
                     /locus_tag="OO326_001558"
     CDS             309..899
                     /gene="uidR"
                     /locus_tag="OO326_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5965981.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1287..2990
                     /gene="uidA"
                     /locus_tag="OO326_001559"
     CDS             1287..2990
                     /gene="uidA"
                     /locus_tag="OO326_001559"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5965982.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            2996..4021
                     /gene="uidB"
                     /locus_tag="OO326_001560"
                     /pseudo
     CDS             2996..4021
                     /gene="uidB"
                     /locus_tag="OO326_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4060..5325
                     /gene="uidC"
                     /locus_tag="OO326_001561"
     CDS             4060..5325
                     /gene="uidC"
                     /locus_tag="OO326_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5965983.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5551..7059)
                     /locus_tag="OO326_001562"
     CDS             complement(5551..7059)
                     /locus_tag="OO326_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5965984.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7160..8335)
                     /gene="manA"
                     /locus_tag="OO326_001563"
     CDS             complement(7160..8335)
                     /gene="manA"
                     /locus_tag="OO326_001563"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5965985.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8534..10180
                     /gene="fumB"
                     /locus_tag="OO326_001564"
     CDS             8534..10180
                     /gene="fumB"
                     /locus_tag="OO326_001564"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5965986.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10323..11726
                     /gene="fumC"
                     /locus_tag="OO326_001565"
     CDS             10323..11726
                     /gene="fumC"
                     /locus_tag="OO326_001565"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5965987.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11723..12652)
                     /gene="tus"
                     /locus_tag="OO326_001566"
     CDS             complement(11723..12652)
                     /gene="tus"
                     /locus_tag="OO326_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5965988.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12728..14029)
                     /gene="rstB"
                     /locus_tag="OO326_001567"
     CDS             complement(12728..14029)
                     /gene="rstB"
                     /locus_tag="OO326_001567"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5965989.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14208..15527)
                     /locus_tag="OO326_001568"
     CDS             complement(14208..15527)
                     /locus_tag="OO326_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5965990.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15752..16495)
                     /gene="rstA"
                     /locus_tag="OO326_001569"
     CDS             complement(15752..16495)
                     /gene="rstA"
                     /locus_tag="OO326_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5965991.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16624..16959
                     /locus_tag="OO326_001570"
     CDS             16624..16959
                     /locus_tag="OO326_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5965992.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(16956..17678)
                     /gene="folM"
                     /locus_tag="OO326_001571"
     CDS             complement(16956..17678)
                     /gene="folM"
                     /locus_tag="OO326_001571"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5965993.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17715..19097)
                     /locus_tag="OO326_001572"
     CDS             complement(17715..19097)
                     /locus_tag="OO326_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5965994.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19283..20227)
                     /locus_tag="OO326_001573"
     CDS             complement(19283..20227)
                     /locus_tag="OO326_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5965995.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20751..22283
                     /gene="pntA"
                     /locus_tag="OO326_001574"
     CDS             20751..22283
                     /gene="pntA"
                     /locus_tag="OO326_001574"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5965996.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22294..23682
                     /gene="pntB"
                     /locus_tag="OO326_001575"
     CDS             22294..23682
                     /gene="pntB"
                     /locus_tag="OO326_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5965997.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23707..24741)
                     /gene="tqsA"
                     /locus_tag="OO326_001576"
     CDS             complement(23707..24741)
                     /gene="tqsA"
                     /locus_tag="OO326_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5965998.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25152..25517
                     /gene="mdtJ"
                     /locus_tag="OO326_001577"
     CDS             25152..25517
                     /gene="mdtJ"
                     /locus_tag="OO326_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5965999.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25504..25833
                     /gene="mdtI"
                     /locus_tag="OO326_001578"
     CDS             25504..25833
                     /gene="mdtI"
                     /locus_tag="OO326_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5966000.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25872..26693)
                     /locus_tag="OO326_001579"
     CDS             complement(25872..26693)
                     /locus_tag="OO326_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5966001.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26793..26876)
                     /locus_tag="OO326_001580"
     CDS             complement(26793..26876)
                     /locus_tag="OO326_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966002.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(26969..27304)
                     /locus_tag="OO326_001581"
     CDS             complement(26969..27304)
                     /locus_tag="OO326_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5966003.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27701..28954)
                     /locus_tag="OO326_001582"
                     /pseudo
     CDS             complement(27701..28954)
                     /locus_tag="OO326_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29061..29990
                     /locus_tag="OO326_001583"
     CDS             29061..29990
                     /locus_tag="OO326_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5966004.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30125..31345
                     /gene="mlc"
                     /locus_tag="OO326_001584"
                     /gene_synonym="dgsA"
     CDS             30125..31345
                     /gene="mlc"
                     /locus_tag="OO326_001584"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5966005.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31470..32165
                     /gene="bioD"
                     /locus_tag="OO326_001585"
     CDS             31470..32165
                     /gene="bioD"
                     /locus_tag="OO326_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5966006.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32118..33410)
                     /gene="clcB"
                     /locus_tag="OO326_001586"
     CDS             complement(32118..33410)
                     /gene="clcB"
                     /locus_tag="OO326_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5966007.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33569..34183)
                     /gene="dmsD"
                     /locus_tag="OO326_001587"
     CDS             complement(33569..34183)
                     /gene="dmsD"
                     /locus_tag="OO326_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5966008.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34226..35080)
                     /locus_tag="OO326_001588"
     CDS             complement(34226..35080)
                     /locus_tag="OO326_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5966009.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35082..35699)
                     /gene="dmsB"
                     /locus_tag="OO326_001589"
     CDS             complement(35082..35699)
                     /gene="dmsB"
                     /locus_tag="OO326_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5966010.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35710..37748)
                     /locus_tag="OO326_001590"
                     /pseudo
     CDS             complement(35710..37748)
                     /locus_tag="OO326_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37809..38918)
                     /locus_tag="OO326_001591"
     CDS             complement(37809..38918)
                     /locus_tag="OO326_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5966011.1"
                     /translation="MINVSHIRTLSSIKSTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38901..>39934
                     /locus_tag="OO326_001592"
     CDS             38901..>39934
                     /locus_tag="OO326_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5966012.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10146 a         9683 c        10230 g         9875 t
ORIGIN      
        1 tcctttgctc gcctgcagcc agctgggtaa gcggacaaat tctcaccgtc tccggtggtg
       61 gggtacagga gctcaattaa tacactaacg aaccggtaaa cggtcgtatg tgttgtttac
      121 cgggatagcg tgtgcaattc atcaacgtct ctgctaaaca actggcagcc aaatcacggc
      181 tattggttaa ccaattgctg ggtgaaaagt atacgaatag agtgtacctt cgcactattc
      241 aacagcaatg ataggcgctc acctgacaac gcggtaaact agttattcac gctaactata
      301 atggtttaat gatggataac atgcagactg aagcacaacc gacacggacc cggatcctca
      361 atgctgccag agagattttt tcagaaaatg gatttcacag tgcctcgatg aaagccatct
      421 gtaaatcttg cgccattagt cctgggacgc tctatcacca tttcatctcc aaagaagcct
      481 tgattcaggc gattatctta caggaccagg agagggcgct ggctcgtttc cgggaaccga
      541 ttgaagggat tcattttgtt gactatatgg tcgagtctat tgtctctctc acccatgaag
      601 cctttgggca acgggcgctg gtggtcgaaa ttatggcgga agggatgcgt aacccacagg
      661 tcgccgccat gcttaaaaat aagcatatga cgatcactga atttgttgcc cagcggatgc
      721 gtgatgccca gcaaaaaggc gagataagcc cagacatcaa cacggcaatg acttcacgtt
      781 tactgctgga tctgacctac ggtgtactgg ccgatatcga agcggaagac ctggcgcgtg
      841 aagcgtcgtt tgctcaggga ttacgcgcga tgattggcgg tatattaacc gcatcctgat
      901 cctctcattt cgcgggctgg tgataactgt gcccgcgttt catatcgtaa tttctctgtg
      961 caaaaattat cctccccgtg ttcggagaat tcccccccaa aatattcacc gtagccatat
     1021 gtcatgagag tttatcgttc ccaacactct cgaacgaacg ttcgattgct tattttatgg
     1081 cttctgtcaa cgctgtttta aagattaatg cgatccatat cacgctgtgg gtattgcagt
     1141 ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc atttctcttc catgggtttc
     1201 tcacagataa ctgtgtgcaa cacagaattg gttaactaat cagattaaag gttgaccagt
     1261 atttttatct taatgaggag tcccttatgt tacgtcctgt agaaacccca acccgtgaaa
     1321 tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc
     1381 agcgttggtg cgaaagcgcg ttacaagaaa gccgggcaat tgcagtgcca ggcagtttta
     1441 acgatcagtt cgccgatgca gatattcgta attatgtggg caacgtctgg tatcagcgcg
     1501 aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca
     1561 ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata
     1621 cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg
     1681 tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg
     1741 aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc gggatccatc
     1801 gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc
     1861 atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgata
     1921 tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcaccagcg
     1981 ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac
     2041 tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgctgcgc gtcggcatcc
     2101 ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg
     2161 gctttggtcg tcatgaagat gcggatttgc gaggcaaagg attcgataac gtgctgatgg
     2221 tgcacgatca cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc
     2281 cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa
     2341 ctgcagctgt cggctttaac ctctctttag gcattggttt cgaagcgggc aacaagccga
     2401 aagaactgta cagcgaagag gctgtcaacg gggaaactca gcaggcgcac ttacaggcga
     2461 ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca
     2521 acgaaccgga tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa
     2581 cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc
     2641 acaccgatac catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggttggt
     2701 atgtccaaag cggcgatttg gaaacggcag agaaggtact ggaaaaagaa ctgctggcct
     2761 ggcaggagaa actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg
     2821 gcctgcactc aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata
     2881 tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg
     2941 ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg taacaattga cctacatggg
     3001 cctcgggctt tgctacagcc tggtgaatat tccttatggt tcacttgcta ccgcgatgac
     3061 ccaacaacca caatctcgcg cccgtctggg cgcggctcgt gggattgccg cttcattgac
     3121 ctttgtatgc ctggcatttc tgataggacc gagcattaag aactccagcc cggaagagat
     3181 ggtgtcggta taccatttct ggacgattgt gctggtgatt gccggaatgg tgctttactt
     3241 catctgcttc aaatcgacgc gtgaaaatgt ggtacgtatc gtggcgcagc cgtcattgaa
     3301 gatcagtttg caaaccctga aacggaatcg cccgctgttt atgttgtgca tcggtgcgct
     3361 gtgtgtgctg atttcgacct tcgcggtcag cgcctcgtcg ttgttctacg tgcgctatgt
     3421 gttaaatgat accgggctgt tcactgtgct ggtactggtg caaaacctgg ttggtactgt
     3481 ggcatcggca ccgcttgtgc cggggatggt cgcgaggatc ggtaaaaaga ataccttcct
     3541 gattggcgct ttgctgggaa cctgcggtta tctgctattc ttctgggttt ccgtctggtc
     3601 actgccggtg gcgttggttg cgttggccat cgcttcaatt ggtcagggcg ttactatgac
     3661 tgtgatgtgg gcactggaag ctgataccgt agaatacggt gaatacctga ccggcgtgcg
     3721 aattgaaggg ctcacctatt cactattctc atttacccgt aaatgcggtc aggcaatcgg
     3781 aggttcaatt cctgccttta ttttgggatt aagcggatat atcgccaatc aggtgtaaac
     3841 gccggaagtt attatgggca tccgcacatc aattgcctta gtaccttgcg gatttatgct
     3901 actggcattc gttattatct ggttttatcc gctcacggat aaaaaattca aagaaatcgt
     3961 ggttgaaatt gataatcgta aaaaagtgca gcagcaatta atcagcgata tcactaatta
     4021 atattcaata aaaataatca gaacatcaaa ggtgcaacta tgagaaaaat agtggccatg
     4081 gccgttattt gcctgacggc tgcctctggc cttacctctg cttacgcggc gcaactggct
     4141 gacgatgaag cgggactacg catcagactg aaaaacgaat tgcgcagggc tgataagccc
     4201 agtgctggcg cgggaagaga tatttacgca tgggtacagg gaggattgct cgatttcaat
     4261 agtggttatt attccaatat tgttggcgtt gaaggcggag cgtattatgt ttataaatta
     4321 ggtgctcgtg ctgatatgag tacccggtgg tatcttgatg gtgataaaag ttttggcttt
     4381 gccctggggg cagtaaaaat aaaacccagt gaaaatagcc tgcttaaatt aggtcgcttc
     4441 gggacggatt atagttatgg tagcttacct tatcgtattc cgttaatggt tggcagttcg
     4501 caacgtacat taccgacagt ttctgaagga gcattaggtt attgggcttt aacgccaaat
     4561 attgatctgt ggggaatgtg gcgttcacga gtatttttat ggactgattc aacaaccggt
     4621 attcgtgatg aaggggtgta taacagccag acgggaaaat acgataaaca tcgcgcacgt
     4681 tcttttttag ccgccagttg gtatgatgat accagtcgct attctctggg ggcatcggta
     4741 cagaaagatg tttccaatca gatacaaagt attctcgaga aaagcatacc gctcgacccg
     4801 aattatacgt tgaaagggga gttgctcggc ttttacgcgc agctcgaagg tttaagtcgt
     4861 aataccagcc agcccaatga aacggcgttg gttagtggac aattgacctg gaatgcgccg
     4921 tggggaagcg tatttggcag tggtggttat ttgcgccatg caatgaatgg tgccgtggtg
     4981 gataccgaca ttggctatcc cttttcatta agtcttgatc gtaaccgtga aggaatgcag
     5041 tcctggcaat taggtgccaa ctaccgtgta actccgcaat ttacgctgac atttgcaccg
     5101 attgtgactc gcggctatga atccagtaaa cgagatgtgc ggattgaagg cgcgggtatc
     5161 ttaggtggta tgaactatcg ggtcagcgaa gggccgttac aagggatgaa tttctttctt
     5221 gctgccgata aagggcggga aaagcgcgat ggcagtacgc tgggcgatcg cctgaattac
     5281 tgggatgtga aaatgagtat tcagtatgac tttatgctga agtaagaaat aacgccggag
     5341 agaaaaacct ccggcgtttc agattgttga caaagtgcgc gttttttatg ccggatgcgg
     5401 cgtaaacgcc ttatccagcc tacaaaaact cataaattca aagtgttgca ggaaaaggta
     5461 ggcctgataa gcgtagcgca tcaggcaatc tctggtttgt tttcagatga aaacgccgga
     5521 gtgaaaattc tccggcgttt tggccgtgaa ttactgctgc ggaattgccg gtacagccgg
     5581 aacgttaaga gctggcatcg caaacatgcc aacaaaatct tctaacgaca ttttctgccc
     5641 atttaacgtt atctgaccgt tagcatattg caggctggtg gtgatggtat tgtcctgcaa
     5701 ggtggtcaga cggaacatct gccccattgc tgatgcacct tcaacttgct gtttcgccag
     5761 ttttttcgct tgatcttcct gataaccttc cagcttcgct acctgagtca tcaactcagt
     5821 tgccatatcc accggaatgg tcagtttcgc atccagagat ttaaccgaac gatctacttc
     5881 ctgcgccagc gtttgcggcg cttctttagt cgttgccgga tctttcagga acagcgacag
     5941 attcagcgca ctttcaccct gactgttttt ccagcttagc ggcgcgatag taatcaccgg
     6001 atcgcctttc aacatcagcg gcagggcgct aaagaaggct tccgtcactt tctcctgata
     6061 aagttcggga ttgttggcaa tttctggctg tgccagcagc gcctgagttt gcgcgttata
     6121 ttgctggcta aactgatgcc aggcttcacc atcaatctgg ccgactttta aagtcagctt
     6181 gccgctgccc agatcctgat tctgtacctt caggctgttt agcgagtaat ccagttggct
     6241 attgatcgtt ttaccgtcat tgaccagatc cgatttaccg ctgatctcca tgccttccag
     6301 cagtgccagt tctttgcctt ccactgaaat ggtcattttt tccagtgaca gtttttggtt
     6361 tcctacgcgc tcaccaaaac ttcccagcgt gctggaaccg tcggttttca gattattaaa
     6421 ggtcaactgc actttctggt tgtattcgtt aactgcgtct atccgaccac tttgcgcctc
     6481 tccggaaagg gagatggctt tgccgtctct gtcagcattt aactggaact cgccgccgct
     6541 aaaggcgact ttttcatcct tttgctcgta attcagtgga ttgagcgaaa tatcggaact
     6601 ggaatcaccg ctgtaaccaa tgcgcgagtt aatctcaaaa ggcgtttcac cttttgccat
     6661 atcaaacagt ggtttgctta cttcgttatt aaccagcgtg gtttgaattg atgccatcga
     6721 cgggatcagg ttcagttttt taagctgggc aagcgggaag ggaccatgat caaccgattc
     6781 gttgaagatg acgctctgac cgcttttaat ccacggattt tctttcccgg caatgggttt
     6841 caccaacagt tgcaactggc tgctgaatac gccgcgatga tagttttgat aactcacttc
     6901 caggttggat tcaggagctg tcagtttgag ttgcgcgttc gcctgcgcga ccatgtcttc
     6961 gagatgggtt tcaatcttct tgcctgtata ccatgcgccg cctgtccaga ctacgcctag
     7021 cgcaacaatg acgcctaccg ctaccagcga tttattcata atgattatcc ataaaatgaa
     7081 atcaggcgga ctggccgcct gaaggtgtta taagccttta ataagcttag caagagatgt
     7141 taattttttc agtaagctct tacagcttgt tgtaaacacg cgctaaacgg ccgtggcctt
     7201 tgacagtcac cggtgattcg ttggcggcaa taaacgctga ttcacccggt ttaagctgta
     7261 actgctgaga acctttccac aacgttgcat cgccttcgac gcagaacaaa atggcggcac
     7321 tctgctggct gatggtggtt tctttatcac taaggtcatg cagcgagaag gcaaaatcat
     7381 ccacaggaat cgggaagtcc agttctgcac cttgtttcac cggctgggtc aacaactggt
     7441 tagccggttt ggcttcgaat ttcacattgg caaccagttc cggaatatca atgtatttag
     7501 gcgtcagacc tgcacgcagc acgttatcgg agttcgccat cacttccagc gccacgcctt
     7561 gcaggtaagc gtgcggtgtt tcagcgaaca ggaacatcgc ttcgccagga ttcaatttca
     7621 ccacattcag taacagcggg gagaacaggc cgctgtcttc cgggtaaaat tcagaaatta
     7681 aacgaatcgt ttgccacggt tcaccctgct gactgtcgag cgccgatttt aaaatcgcca
     7741 gcgcgcggga tttttcttca ccctgcatat tcaacaggtt ggcgaacagt tcgcttaaac
     7801 gttcggcatg tggctgttgt aaaaagtgag caatcgccgg atgtgcaccg gcgaccggct
     7861 ggagtaggga gacaatctcg gaaaattcac gaaacgcgtt catcgcaagg aaaggcgtca
     7921 gcgcaaaaac cagctccggc ttgtggttag gatctttata gttacgctcg gcggcatcca
     7981 tcgggatacc tgcggcattt tctttggcaa aaccgatttc agaattgtgt ttgtttggat
     8041 gaacctgaat ggagagtggc tgtgctgcgc ataatacttt gaacaggaaa ggcagttcgc
     8101 caaagcgttt ggcaacggcc tctccgagca gagtcgattt atcactctca atcacatcac
     8161 gcagtgaaac gatatctccg gcggcattct gcactcgtga actgcttttc ggatgtgcgc
     8221 ccatccacag ctcggccatc ggctggctgg acggattttc cataccataa agttcagtca
     8281 acgccgtttt gctaccccag gcatagtttt gcactgagtt aatgagtttt tgcatgatca
     8341 atccctgttt taatgtggaa attaatcccg ctattaaagc aagaatccta cgggaagtaa
     8401 cctggagccg caaaaagtcg tactagtctc agtttttgtt aaaaaactgt gtaggatatt
     8461 gttactcgct tttaacaggg caacggaaca cccgcccaga gcataaccaa accaggcagt
     8521 aagtgagaga acaatgtcaa acaaaccctt tcattatcag tctccttttc cactcaaaaa
     8581 aaatgatact gagtattacc tgctaaccag tgaacacgtt agcgtatctg aatttgaagg
     8641 gcaggagatt ttgaaagtcg cacccgaagc gttaactctg ttggcgcgtc aggcgtttca
     8701 tgatgcgtcg tttatgctgc gtccggctca ccaacaacag gtggccgaca ttctgcgtga
     8761 cccggaggcc agcgaaaatg ataaatatgt ggcgctgcaa ttcctgcgta actccgacat
     8821 cgcggcgaaa ggcgttctgc caacctgtca ggataccggc accgcgatta ttgttggtaa
     8881 aaaagggcag cgtgtatgga ccggtggtgg tgatgaagcg gcgctggcgc gcggtgtcta
     8941 taacacttat atcgaagata atctgcgcta ctcgcaaaac gcgccgctgg atatgtataa
     9001 agaagtgaat accggcacca atctgccagc gcagatcgat ctttatgccg ttgatggcga
     9061 cgaatacaaa ttcctctgta tcgcaaaagg tggtggttcg gcaaacaaga cgtatctcta
     9121 tcaggaaacc aaagcgttac tgacgccggg gaaactgaaa aattacctgg ttgagaagat
     9181 gcgcacgctg ggtaccgcgg cctgtcctcc gtatcatatt gcgttcgtta ttggtggaac
     9241 ttctgcagaa acgaacctta aaacggtgaa actggcttcc gcgaaatact atgatgaact
     9301 gccaacggaa gggaatgagc acggtcaggc gttccgcgat gtggaactgg aaaaagaatt
     9361 actgatcgaa gcgcaaaatc tcggtctggg tgcgcagttt ggtggtaaat acttcgctca
     9421 cgacatccgc gtgattcgcc tgccacgtca cggcgcatcc tgtccggttg gtatgggtgt
     9481 ttcctgctct gctgaccgta atatcaaagc gaagatcaac cgtcagggga tctggatcga
     9541 aaaactggaa cataatccag gtaaatatat ccctgaagag ctgcgtaaag cgggagaagg
     9601 cgaagcggtg cgcgttgacc ttaaccgtcc gatgaaagag atcctcgcac agttgtcgca
     9661 gtatcccgtt tctacacgct tatcgcttaa cggcacgatt atcgttggtc gtgatattgc
     9721 tcacgccaaa ctgaaagagc ggatggataa cggtgaaggg ctgccgcaat acattaaaga
     9781 tcatccgatt tactacgcgg gtccggccaa aacgccggaa ggttatgcct ccggttctct
     9841 tggcccaacg accgccggac ggatggattc ttatgtcgat caactgcaag cgcagggcgg
     9901 aagtatgatc atgctggcga aaggcaaccg cagccagcag gtgacggatg cctgtaaaaa
     9961 acacggcggc ttctaccttg gcagtatcgg tggtccggcc gctgtattgg cgcagggaag
    10021 tattaagagc ctggaatgtg ttgaatatcc ggaactggga atggaagcca tctggaaaat
    10081 tgaagtggaa gatttcccgg cgtttatcct tgtggatgat aaaggaaatg acttcttcca
    10141 gcagatacaa ctcacacagt gcactcgctg tgtgaaataa acagagccgc ccttcggggc
    10201 ggttttttta catggcacga aagaccaaac atttgttatc aaatagtaaa taatgagtga
    10261 gctaaaagtt gcttaacgaa agcaaaacag aaagaaaaaa ttaatcaggt gaggagcagg
    10321 ccatgaatac agtacgcagc gaaaaagatt cgatgggggc gattgatgtc ccggcagata
    10381 agctgtgggg cgcacaaact caacgctcgc tggagcattt ccgcatttcg acggagaaaa
    10441 tgcccacctc actgattcat gcgctggcgc taaccaagcg cgcagcggca aaagttaatg
    10501 aagatttaga cttgttgtct gaagagaaag cgagcgccat tcggcaggcg gcggatgaag
    10561 tactggcagg acagcatgac gacgaattcc cgctggctat ctggcagacc ggctccggca
    10621 cgcaaagtaa catgaacatg aacgaagtgc tggctaaccg ggccagtgaa ttactcggcg
    10681 gtgtgcgcgg gatggaacgt aaagttcacc ctaacgacga cgtgaacaaa agccaaagtt
    10741 ccaacgatgt ctttccgacg gcgatgcacg ttgcggcgct gctggcactg cgcaagcaac
    10801 tcattccgca gcttaaaacc ctgacacaga cgctgagtga aaaatcgcgt gcatttgccg
    10861 atatcgtcaa aatcggtcga acccacttgc aggacgccac gccgctaaca ctagggcagg
    10921 agatttccgg ctgggtagcg atgctcgagc ataatctcaa acatatcgaa tacagcctgc
    10981 ctcacgtagc ggaactggct cttggcggta cagcggtggg tactggacta aatacccatc
    11041 cggagtatgc gcgtcgcgta gcagatgaac tggcagtcat tacctgtgca ccgtttgtta
    11101 ccgcgccgaa caaatttgaa gcgcttgcga cctgtgatgc cctggttcag gcgcacggcg
    11161 cgttgaaagg gttggctgcg tcactgatga aaatcgccaa tgatgtccgc tggctggcct
    11221 ctggcccgcg ctgcggaatt ggtgaaatct caatcccgga aaatgagccg ggcagctcaa
    11281 tcatgccggg gaaagtgaac ccaacccagt gtgaagcatt aaccatgctc tgctgtcagg
    11341 taatggggaa cgacgtggcg atcaacatgg gtggcgcttc cggtaacttt gaactgaacg
    11401 tcttccgtcc gatggtgatc cataatttcc tgcaatcggt gcgcttgctg gcagatggca
    11461 tggaaagttt taacaaacac tgcgcagtgg gtattgaacc gaatcgtgag cgaatcaatc
    11521 aattactcaa tgaatcgctg atgctggtga ctgcgcttaa cacccacatt ggttatgaca
    11581 aagccgcgga gatcgccaaa aaagcgcata aagaagggct gaccttaaaa gctgcggccc
    11641 ttgcgctggg gtatcttagc gaagccgagt ttgacagctg ggtacggcca gaacagatgg
    11701 tcggcagtat gaaagccggg cgttaatctg caacatacag gtgcagccgt ggaatgatca
    11761 aacgaagcgg ctgcgcctga ggtttataac ggtgctgcac attgtcggca tcgtaattta
    11821 acaactcacc aacgtccggc acgcccgcgc cattatcccg attaatcagt gcaatcagtg
    11881 gtgtagggca ggcgtgttgg acttgttttt gatctccttt gtaccagacg cgggcaatcg
    11941 gctgcacctt cactggacgt ttgattttta acttcgcgtt ctgtggcagg gcagcgatat
    12001 cctgatactc tcgctccagt tttctttgcc actcctcgcg cgtccacggt gcgacactgc
    12061 gtggtgattt caggcttttt tccagctgtg ccaggacttc atcacgatgc aaattcttaa
    12121 tgatatgttt attagcccaa ccaaagcgta aagtggcggg gtcgtgcaga acggtgagcg
    12181 tgcggtaagc attaagggtg atcagccccg gcaaatgacg atgcacccat tcaaaacgtg
    12241 ccgcggtggg gagttctgat tcaaccgtga cgatatgctc gaacgtggtc ttgagtttat
    12301 tgatgtgctg aatatgactg accaacgctg cttgcgaaag gttatcgacc tggtaacaca
    12361 acacgccagg cagacggaca gcggccttgc tgctgcgatt ttcggactgt tgttgaataa
    12421 ataaatggcg gaaatgacgc aacgccagcg actgcgcgtc gttgccgaga tgttgtttta
    12481 cctcaatacg attaagcgga ttatgctcat cctctttttt tacctccggc aaagagaaca
    12541 cgcgggcaac caatagcttg tgttgctcaa gatgagcggc aaatgtagcc agctcttgtt
    12601 ccatctggcg aaaggtagtg ttgagtcggt ctacgagatc gtaacgcgcc ataatattaa
    12661 ccactttagt tacaacatac ttattttata gcacagtcgt ggtgacttga ccatcgtgcg
    12721 cgtagtgtca ggcagaggta aattgcggga tgttatgcca taacggccag ctaaacgaga
    12781 agcgagcacc acccagttcg ctgatgtcac aattaaccgt accgcccatt gtcagtgcta
    12841 ttgagtggac aattgccagc cccagcccgc agccgccggt tgagcgatcc cggctgggat
    12901 cgaggcgaac aaaaggttca aagatatgtt cgcggttttc tggggcaatc cctgggccat
    12961 catcctcaac aattaatgtc gctctattcc ccgacagtag caggctggtt tcaaccgttg
    13021 aatggcagta gcgcagggcg ttattgagca aattatccag cacgcgctcc attaagcgca
    13081 tatccaacgc cgcataatgg ccttgcatga gcgtttttat ccgtaccgtt ttatcgggcg
    13141 ttactgcctg aatatctgcc agatgcgttg acagccacaa cggcaggtct ggttcgctaa
    13201 gatgaagctc gttttgtggg cgatcgagtc gggcataagt cagcagctct tcaattaaag
    13261 cttcaagttg actgatatca cgattcaacg cctgggattc ggcggcgctc aggttatcgc
    13321 tcatctccag tcgataacgc aggcgcacta acggtgttcg cagttcgtga gcgataccgt
    13381 caataagctg ttttttgctg gcaattaagg cgttgatatt gtccgccatc tggttaaatg
    13441 cgataccaag tcgttcaaag ctcgaaccct catcaaagtg gatacgttca ttgagatgcc
    13501 catcgccaaa tcgttgcgcc gctgcttcca gttttaacat atcctgccag tgcggacgca
    13561 tccagataaa caccggaaag gcgagggaaa tagcaataaa agcgatcagg gcgatatcca
    13621 gcaatcgcat ttgatggagg taataaagat aaggaacagg accaactgcc agcacgtagt
    13681 ggctgcgcgg gatacgctgc aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc
    13741 cacgcagtcg gtgcatggaa atatcatcaa gatggtattt actcagtggc tcgacacgca
    13801 gatcgaaaga gagatttaaa tccatctctt tcagcgtctt accccagtcg tgtgggggga
    13861 tctctcgcaa ttcgctgcgc atcagataca gcgaactgtt catcaaatca tccagcgact
    13921 gtttgcccgc gcgttcggcg gtaaatttgt acaccagccc aaccagcaga gacatcacaa
    13981 ggaagcagac aaacaataac aggtaaaact ggataaacaa ttttttcatt ggcatcgccc
    14041 gcaacagaat actattcgta ttaattcacg actgtggagc aatgtaaacc gccaactgga
    14101 taagaccttg tttggaagat aatgtccgga acagacggat gtatccccgg tgtattcata
    14161 ttcagtaaat tcaattttcc accggggctt gtttattatg ctaaggttta atttaacgtg
    14221 acgctttgtt gttctttagt ctctctatcc ttaagaagac gggctgatat tacaccggac
    14281 attattgaac cattcacatt aagcgctgtt cttgccatat caacgatcgg ctctatggca
    14341 ataaaaataa ctactaatgc gacagggaaa ttaagtgttg aaaggacaat cagcgccgcg
    14401 aatgtcccac cactacctac gcctgctacc cctattgagc caagcgcgat ggcaggtaaa
    14461 agagttgcga ggaattgcaa tgagaaggga tcgataccca ttgttggagc aatcatcgcc
    14521 accatcacgg caggataaat cccagcgcag ccattttgtc ccatgctgga accaaaagac
    14581 gctgcaatat tagcaatcgt atttggaacc cccaattttt ccaatgcaga aatagccaga
    14641 ggaattgaag ccgcactact gcgagaaaca aacgcaaagg taagtacttg ccagacggtc
    14701 ttgtaatagt taacggggtt tttacctatt aacagcaaaa tgcccgagtg aacaataaac
    14761 atcacaaaaa cggccagata acaggcgacg atgaacccga gcaaattggt aaactgtgaa
    14821 agctgatacg ttgagaagac tttcgccatt agtgccatta ccccataagg cgtcagcgca
    14881 atgacaatac ggaccatttt cattacccat atttgaatcg cgttgatccc cgcgctcaat
    14941 ttttcgccct ctgctggtgc atctttcttt acttttaaca gcgcaatacc agcaagaatt
    15001 gtaaagatga cgatgccaat aaccgaaaca cttcttgccc cggtcagatc aaggaagagg
    15061 ttggttggga tcaatgacgt aatgagtcgc ggtaaagata ccgcagctgt tttcgagacg
    15121 tcatcgctgg ttaaagagga ttgcatatga ccaaaggcgc tggcatcaag gccaattgca
    15181 ctggcaatag ccaggccaac taaacctgca atcatgacta aacacaacat acaggcaaca
    15241 atagttaaag acatcttgcc aataccagcg ctattttcga gcttattaat cgctgaaaga
    15301 atcgaaatga atatgagtgg aacggcaaca agttttaata aattaacata accgttacca
    15361 gcaatattat accattcgat aacggtttca ctgcttgtct caggaatgaa atgcagggag
    15421 ataccaatga tagcccccag gaaaatcgcg ataaaaacac ttttggaaag ggattcgctc
    15481 ttttgatccg ttttgaaaat cccccatgag cagatcaaaa aaagcacaat atatgtgaaa
    15541 gttgtaaccg ttttttaagc ctcttgcctc attcagcacc tgaaatgccg gaaagagttt
    15601 gttgtaaaaa atctgcgcca taagacgcag ggacgaggcg attatagaga aattttttct
    15661 cattttcata tcaaatgtag ggggtttgga atttgaataa gctcttatta attgaattcg
    15721 ttaattgttc tctttttcag atgaacaaat attagtcatc tgattgttta ttgtcactat
    15781 cccatgcatg aggcgcaaaa agatagcctt tgttacgcac agttttaatg cgataaggtt
    15841 ctgcggcgtt atcgagcagt ttttttctta accgcgaaat agccacgtcc acgctacgat
    15901 ccagtccgtc ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt
    15961 gcccggcatg cgtagctagt tcccacaata attcgaaatc agctgtcgag agcgagattt
    16021 cagtgttagc cagggtgact acgcggttga tggggtcgat agttaacgtg ccgaaatgca
    16081 gggctttgta gggagtaaga gacgtttcct gaagaccttt ggtcagtgtg gcttgctcat
    16141 tctgacgcaa atgcaaacgt aaacgcgcta gcaaaacagc agggggcgtc gttttgagaa
    16201 tatagtcgca ggcacccatt tccagtgcca ggatgtggtt catatcgcta tcgagagagg
    16261 ttagaagaac aatcggtcca gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt
    16321 ccttgcctgg tagcatgatg tcgagtaaca ccaaatccgg attttctcgc aaaatggttt
    16381 cttcggcctg gtcgccgcgc ggttctacgg taacctgcat atcatgtttt gccaggtacg
    16441 cggcaatcag tgaaccgact tccgcatcat cttccacaaa tacgatagtg ttcataacat
    16501 tcacggtaga tataaaaacg tcacaataca ccgcgctgtt tttacttact atcaatcgtt
    16561 tctaaacaag ttttcatcag gtattctact gatgacttgt tatggaagtg ttaaggtaaa
    16621 aagatggggc tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt
    16681 ttagcaaaaa cgaaaaatta ttatatcgcc gggctgattc cacttttccc gacctttgcg
    16741 cttatcgcgc attatattgt tgccagcgaa cgcggcattg aagccttacg cgcaaccatc
    16801 atttttagta tgtggtcgat tattccctat tttgtttacc tggtgtcgct gtggtatttc
    16861 accggaatga tgcgcttgcc cgccgctttt gttggttctg ttgcgtgttg ggggataagc
    16921 gcatgggtgt tgattatatg ctggataaag ctgcattaac gcagatgacg accgccatca
    16981 agtgggaaac tgcgtccggt gacaaagcaa ctggtaagta agtaatcgac caggtcgatc
    17041 acttctttct cgccaggcgc ggttttcatc agtgatttat tcagcgcctg ttgtcgatat
    17101 tcggcatcat catgttcatt aaacaggatc agcgatggcg caatagaatt cactttcact
    17161 tccggtgcca gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc tttgcttgca
    17221 gcatacgcaa catgtttgtc gctaccgcgc tccaccacat aatcggtaaa gtgaatgata
    17281 tcgctggcgg cgtgtccgtg cccacgcagt aatctttcca gcgcatggtt gagcaggtat
    17341 ggggtattaa cgtggatctg catcatgcaa gccagtacgt cggccagtgg cgcacccggt
    17401 ttttccgcaa tccacgcact ggcgttatgc aaaatagcac gcagaccatg ggtggttttt
    17461 agtacttcat cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc ctgaatacac
    17521 tgcgcacctg cattaatcag tccatcaatg gctggatagt gtgtccgata gctgacaatc
    17581 accggttgct tttgattaat gaaatgccat gcgagggcga ggccgatgcg acgacctccg
    17641 ccagtaatta atattggcaa gggctgggtt ttacccatcg ttatctcctt tgctatccaa
    17701 cgatgggatg aacgttatcc caccagcatc caggtcgccg gaactgaggc aattaacagc
    17761 atgccaatca gcaccatttc ctgacggttc agtacattat catgtgtatg cgttttgcgc
    17821 gcatacagga aaaccagcag ccccggtgca tacagaacaa cggataacag caagtgcatt
    17881 ggaccagagg cgtacaataa ccataagcca taaatgcagg caccgacacc taccgcttta
    17941 tgcagtggac gggttgcgat tttcagcaag aacgcgccga ccaggaaata gggcaccaga
    18001 atcatttctg aggcgatggt cagcaacgtg ttgtaatcgg aaccggtcag ccagatgagt
    18061 accagacaaa tttgcacgca gatgttggtc aaccacagtg aggcggatgg cgcagcttgt
    18121 gcgttctggc gcgcaaaaat gcgtgggaat gctttatgag tggcagccag gaacggaact
    18181 tctgccgcca tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat caaaccggca
    18241 gcgatgatga tttcgcccca tggtcccatc atttccacca tcagaccggc cattgacggg
    18301 ttacgaattt cagccagttc aggacgtgcc accacaccca gcgaaagcag cgttaccagc
    18361 aagtaaacgc ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac atcacgttta
    18421 ttacgcgcac gcgcagaaac aaccacggca ccttccacac caatgaaaac ccacagggtg
    18481 atcagcatgg tgtttttcac ctgttcccaa acaggtacgc caagtgcaag tccggtgaag
    18541 tcgagcttga aggtatccag tttgaacatc atcatcgcca gcacaacaaa cagacccagc
    18601 ggcaacaatt ttgccagtgt cgccaccagg ttaatgctgg cagcggtttg caccccgcgc
    18661 aggatcaaaa agtgaacaat ccacaataaa gccgatgcac cgactatcga ttgccaggta
    18721 ttaccatcgc caaacaggcg caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa
    18781 acgatcacca gataggagac gttggcgatg accgcgcaca gccagtatcc ccatgcggaa
    18841 caaaagccga ttagctcgcc aaacccttcg cgggcatagg taaagatacc gccatcaagt
    18901 tcgggacgaa tgcgcgtgag gatcagcatg gcaaaggcca gcaataaaat gccagcgcca
    18961 gtaatacccc agccgatgag cagtgctgcc gggctggcaa ctgccgccat attttgcggc
    19021 agactgaaaa cacccgcgcc cagcattgag cttaatacca gcgcggtgag tgcgctcagt
    19081 cccagtttct tttccatcga tatccagtga ttaaaacgtg ataagaagaa taaatattag
    19141 ggcaaaacgc ataaaaatgg agtttgccac tgaagcgcgg gattttacga agagatgtgt
    19201 ctgcatgcaa tggtcgcggg gaaatttgtg actaaaaatg cgaaaggcgg catcaagccg
    19261 ccttatagga gtaactgaat agttatttat acagatctgc gctgacggtc aggttattac
    19321 cacgtttctg ccactggcgg gtgatgtggt aatacttagc acctttcttc gcggcacgtt
    19381 tcgcaacctg ataggagact tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt
    19441 cgaacgggac catcatcgct gctgtcgctt tgttcagttc ttcgacttta gtgccatccg
    19501 ggagcgtgac ggtgtaacgc ccgccttttg atgactgggt ttcaaagaag cgaccgactt
    19561 cagaacttgg tgatgcggta gtcgcaacac ccgggatctc aactttcttc gcggcttcgc
    19621 caccggcagc cagagctgca cgtcctgctt cggaatctgc cgggatcaca tccgggctct
    19681 ggacgatacg tttcttagca tcttttttat agatgaatgc agtaatacgc tggttgccgc
    19741 cctggttggc atcgatttga cgaacgatgt agaaagagta ggcacctttc gcttttgccg
    19801 ctttggtgat ggcatcattg acttctggct ggctacgata gaagccctgg acggtgaccg
    19861 tgtcaaacgg ttcaatcaga acagcctgat ctttcggcag ttcgacaaca ccgttaatta
    19921 cgcgattact tgtttcttct gctttttcag catcggcttt atagaggtca gcgaccacac
    19981 gccagttacc gctgttacca aaatcagaag tgtcgacaac ataaaaagag gcggcacctt
    20041 ctttatctgc gcgacgagaa acggctttca ccgcttcgcc aatagcatta aaacgaccgg
    20101 taaccactac acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg
    20161 ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa
    20221 gcttcataaa aataatcctt cgccttgcgc aaaccaggta ctggtattgt tattaacgag
    20281 aaacgtggct gattattgca tttaaacggt gtaactgtct gcgtcatttt tcatatcaca
    20341 ttccttaaac caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat
    20401 cagcatcatt actactgaag caactgaatt gtataagtta atttaatgtt aagtagtgat
    20461 tcgtgccggg gcgatgtctc gttttacccg accgtcgaag acaattatca gtctttatcc
    20521 ggcgttctaa ggtgtttatc ccactatcac ggctgaatcg ttaatatttt gcgagttcac
    20581 gccgaaatac tgatttttgg cgctagatca caggcataat tttcagtacg ttatagggcg
    20641 tttgttacta atttatttta acggagtaac atttagctcg tacatgagca gcttgtgtgg
    20701 ctcctgacac aggcaaacca tcatcaataa aaccgatgga agggaatatc atgcgaattg
    20761 gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca aaaacagtgg
    20821 aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt caactggcaa
    20881 gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct
    20941 ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga
    21001 atcccgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa
    21061 aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt atctcacgcg
    21121 cacaatcgct ggacgcacta agctcgatgg cgaatatcgc cggttatcgc gccattgttg
    21181 aagcggcaca tgaatttggg cgcttcttta ccgggcaaat cactgcggcc gggaaagtgc
    21241 caccggcaaa agtgatggtg attggtgccg gtgttgcagg tttggccgcc attggcgcag
    21301 caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag
    21361 ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagcg ggcagcggcg
    21421 atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg
    21481 cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc
    21541 cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc
    21601 tggcagccca aaacggtggc aactgtgaat acatcgtgcc gggtgaaatc ttcagtacgg
    21661 aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg acgcaatcct
    21721 cacagcttta cggtactaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg
    21781 gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg atccgtgcgg
    21841 gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag gcggcacaaa
    21901 aagcggcacc ggaagtgaaa actgaggaaa aatgtgcctg ctcaccgtgg cgtaaatacg
    21961 cgttgatggc gctggcaatc atcctgttcg gttggctgtc aagcgttgcg ccgaaagagt
    22021 ttcttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga
    22081 atgtatcgca cgcgctgcat acaccgttga tgtcggttac caacgcgatt tcagggatta
    22141 ttgttgtcgg agcactgttg cagattggcc agggaggctg ggttagcttc cttagtttta
    22201 tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc
    22261 tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca
    22321 tacattgttg ccgcgatcct gtttatcttc agtctggccg gtctttcgaa acatgaaacg
    22381 tctcgccagg gtaacaactt cggtatcgcc gggatggcga ttgcgttaat cgcaaccatt
    22441 tttggaccgg atacgggtaa tgttggctgg atcttgctgg cgatggtcat tggtggggca
    22501 attggtatcc ggctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc
    22561 ctgcacagct tcgtgggtct ggcggcagtg ctggttggct ttaacagtta tctgcatcat
    22621 gacgcgggaa tggcaccgat tctggtcaat attcacctga cggaagtgtt cctcggtatc
    22681 ttcatcgggg cggtaacgtt cacgggctcg gtggtggcgt tcggcaaact gtgtggcaag
    22741 atttcgtcta aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc ggctctggtc
    22801 gtttccttct tgctgctgat tgtatttgtt cgcacggaca gcgtcggcct gcaagtgctg
    22861 gcattgctga taatgaccgc cattgcgctg gtattcggct ggcatttagt cgcctccatc
    22921 ggtggtgcag atatgccggt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct
    22981 gcggctgcgg gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt
    23041 tcttcggggg ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc
    23101 gttattgcgg gtggtttcgg caccgacggc tcttctactg gcgatgatca ggaagtgggt
    23161 gagcaccgcg aaatcaccgc agaagagaca gcggaactgc tgaaaaactc ccattcagtg
    23221 atcattactc cggggtacgg catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt
    23281 accgagaaac tccgcgctcg tggtattaat gtgcgtttcg gtatccaccc ggttgcgggg
    23341 cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg
    23401 ctggaaatgg acgagatcaa cgatgacttt gctgataccg ataccgtact ggtgattggt
    23461 gctaacgata cggttaaccc ggcggcgcag gatgatccga agagtccgat tgctggtatg
    23521 cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact
    23581 ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt
    23641 gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg
    23701 aggccgtcac tctttattga gatcgcttaa cagaacggcg atgctttgac ctcccgctgt
    23761 ttgttcaagc gcaattttga caataattgt caacggcacg gaaagcagca tacccaccgg
    23821 tcctaacaac catccccaaa aaatcaacga caaaaatacc accaatgtcg aaagccccag
    23881 cccacgtccc atgatgcgcg gctccagaat attgccgaag accagattaa tcagcagata
    23941 tcccgccagc accagcaacg cttcgtagaa gccattaaac accagtacct gagcgatagg
    24001 ggggattgcc gcgagaactg aaccaatatt agggatgtaa ttaagcgcaa aggccagcaa
    24061 tccccagaca aaagcgaagc gaacatcgag tgcggcgagc atcgcccagg cgaccaggcc
    24121 ggtgatgatg ctgatggctg ttttcagcac cagataatga gaaacactgt caatcgcacg
    24181 ttgaatcgcc gccatccctt caaccggacg cgccatcatt tgctgaaatt ttccgggcaa
    24241 ttgtggcact tcgagcagca taaacagcac cgtcagcagc aataaaaata ttgatgacat
    24301 ggcattagat aactgcgtca ataagttggt gagcaacgtc atcgccgcgt tcggatcaat
    24361 gtaatgcgcc agttggtcaa ctgagacgtc aatccctacg cgttgcaaca acggctcaag
    24421 agcttgcagc ggcgtcataa tagagttgcg atattgcggt aacgtccgcg tcaactcgtt
    24481 gagcgcggaa cccagataag ctaatagcaa caccatcgcc atcacgatga ttgtcatcaa
    24541 aatcgacact gccagtacac gcggcacacg ccagcggacc atatgttgca ccagcgggtt
    24601 aagaataaca gcaataaata atgcgagaat aaacggcacg atgatctcgg cggcaaaacg
    24661 gataccgcag agaataatga ccagcattcc caacataatg acgattttta ggccattgag
    24721 cgtgatgatc ggctttgcca tgcttgtccc cgtttttctt cctttgctaa taataatgtc
    24781 tttttcgctc cgtcatcata acgaaaatca aaccgctcag gtaaagaagt gaaaatattt
    24841 tgagttaatt cttaagctat gatacaaatc aggcgtgttc aactaccgag gacaattatc
    24901 atctgcgatg acgagaagca acactgcgga taattgtaat attatggaca atttgttcag
    24961 gacgttattt tctacattta cccatcttcg tacctcatcc actatcttgc tcgtaggtga
    25021 gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct aagcttgccc tatgcgcagg
    25081 gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa attaaaatag ttctcttgca
    25141 ggagaaggac aatgtatatt tattggattt tattaggtct ggctattgct acagaaatta
    25201 ccggtacgct gtcaatgaaa tgggcgagcg tcagtgaggg aaatggcggc tttattttaa
    25261 tgctggtgat gatttctctg tcgtatatat ttctctcttt cgccgttaaa aaaatcgcct
    25321 taggcgtagc ttatgcgctg tgggaaggta tcggtatttt atttattacc ttgtttagcg
    25381 ttttgttatt cgacgaaagt ttatcgctga tgaaaattgc cgggttaacc accctggtcg
    25441 ccgggatagt gttgatcaaa tcaggtaccc gtaaagcgcg taaacctgaa ctggaggtga
    25501 accatggcgc agtttgaatg ggttcacgcc gcctggctgg cattggcaat cgtgctggaa
    25561 atcgttgcta acgtcttttt gaaattttct gacggctttc gtcgcaaaat atttggcttg
    25621 ctctccctgg cggcggtgct ggcggccttt agtgcgcttt ctcaggccgt taaagggatc
    25681 gacttgtctg tcgtttatgc attgtggggc gggtttggta ttgccgccac gttagccgca
    25741 ggttggatct tgtttggtca acggttaaat cgtaaaggct ggattggcct ggtcttgctg
    25801 ttggctggaa tgatcatggt gaaacttgcc tgatgaagac gctgcccgcg ctgtcgggca
    25861 gcgtttgaac attatttttg cgacagttga tccagcttgt cgcgaaaacc ggtaacagaa
    25921 atggcccggt tatcggcgcg ccagcgatct ttcgcggcag gagccgaact ttgcacccca
    25981 attaattgcc agccgtcatc ggtatgcaac atcagaggcg aaccgctgtc acccggcaag
    26041 gtatcgcact gatgtgacat caccgacgtt tgcgcccagc cagtcacttc acagttttga
    26101 tgactgtata acgtatcgag atgatcttca gggtatcccg cctgagtcac tttacgacca
    26161 gccgatttta atgcggcagt aagcgcggct ttatctccct caaataacgg caacggtgta
    26221 atgccagaag ggggattacg tagcacaatc aatccgaagt cccacggtgc agctgcggga
    26281 ggaacaatcc aaccatcccc atctgctttt aaccgctttc ccagtgtcgg atcaacgcgg
    26341 ccttctatgt cgtggatctc atagcgccaa agacctttat ttgacacaaa acgcagcgct
    26401 accgccttat ccgctttacc cttaggcggc gtcaataaac agtgtcctgc cgttaatgcc
    26461 agattgggtg caatcagcgt cgccgtacat aaattgccgc tggccgtttc cagttgccca
    26521 accgcatccc acggtgattg ggtcgtgtca ttcactggca cacgatcatc atgaccaaaa
    26581 aacagggtgc tgacctcatc gtttgccgat ttggcaacgt ctggtttatc tgcaaacaca
    26641 aaagcagacg tcaaactaat tgcacccaac actacagcaa tggttgtacg catatcacac
    26701 tctggtgggt aattatgatt attaaaagca aaccctcata aatactatag acgggacggg
    26761 atgaaagtgg gagtaaaatc agataactac agtcaggaaa gataaaaacg ggcggcaatc
    26821 agtgcgcata aaataaggat gataagaatg aactcaaagc gataacggcc caccatacta
    26881 ccctccgcta aaggcggtgc taaacgttac ccgcttagca ccgtaatttt caggcgcgag
    26941 ggggcgcgcc agcattactg ttgaaaactt acgctgcggg ttgtgcagca ggttttgccg
    27001 gttgctgatg gccgtgtttc ccggcgtgtt tcttcgctgc ctgcgctttt tgttcagggg
    27061 ctttctgttc agctttcgta tttttatgat gctttttagc cgcctgcgct ttctgggcag
    27121 gtgctgcttt atgctgtttt ttatgatgtg tagttttcgc tggcgctgct ttggtggtcg
    27181 tcgcagtcgg agcaggtgtg gtcgcagtct ctgcagcaaa ggcggcagaa gacagaccca
    27241 tagcagcggc aacaaccaga gctaatactt ttttcattgt cataccctca atttgttttt
    27301 tcatttaacc ccactgcggg gccgttgaaa taactatatc cctgggaatg gcagacttcc
    27361 gtgagtggtt ggtttcagcg tgtaacgata tgtacaaacg ctgaataaat tacagcgttg
    27421 ataataacgt attgtgactt aaggaaaatt tagctaccaa taatagtagt cttgatcggg
    27481 ttaactatta tactacttac aaagcggtag aataactgcg catcagtaat aaatgacaca
    27541 cagcaaaatg aatccgttta tttgggtact tataatcctg atgacgctag atgcgctgcg
    27601 ggaattggct ggcgcttcgt ctattttagg atggctatta acgttggttt gagcaggcaa
    27661 taagtccgga cgggtattta ccgcagtccg gacttatttt tcaggcgtgc agacgacgat
    27721 gcaaacgcgt cccgaccagc agagcaatga ccagcatcag agcaataaat gcgccgacgc
    27781 cgttccagcc atagttatgc cagaaaacac cacccagcgt cccggcaata ctcgacccca
    27841 gatagtaact gaacagatac agcgaggagg cctggccttt agcgcgtttt gcgcgggggc
    27901 cgatccagct gctggctact gagtgggctg cgaagaatcc ggctgagaag agtaacattc
    27961 cggcaaagat cagccacagc gagctaaata aggtcatcag taaaccaaac ggcataactc
    28021 ccgtcgaaaa caacatcacc ggaccgcgcc catagcgggt ggtcatggtt ccggctttgg
    28081 gtgagctcca tgtaccggtc aaataagcca gcgataataa gccaaccacg gcctgactga
    28141 catgctaggg ggaaagcatc aaccgatagc cgatgtaatt aaacagcgtg acgaacgacc
    28201 ccatcagcaa aaagccttct gcgaacaata acggtaatcc ccggtcacgc cagtgcagac
    28261 gaaagttgat aaataacgtc ttagggcgca gcgaagtcgg gcaaaaatgg cgtgattcag
    28321 ggaggatttt ccagaacatc aacgccgagg ccagcgcgaa acaaccgatt gccgccagag
    28381 caattcgcca gttgaaaaag tccgtgaaga caccactaat taagcgtccg ctcatgccgc
    28441 caattgagtt gccgctgata tacaacccca tcgaaaaggc cacgaaactg gggtggattt
    28501 cctcgctaag ataagtcatg ccaacagctg ccacgccact taacgaaagc ccaatcaagg
    28561 cgcgcataat caaaatgccg tgccagctgg tcatcattgt cgaaagtaac gtacaaatgg
    28621 aggccaacag tagcgccgtg accatcactg gtttgcgacc aatggcatcg gatagcgggc
    28681 cagtaaatag caaaccgata gccaacatcg ccgtggaaat ggacagtgaa atactactgt
    28741 tcgcgggcgt taagccaaac tcctgcgaaa gcaccggaag gataggctgc acacaataga
    28801 gaagtgcaaa ggttgccagt ccggcagaga acagcgccag ggtgacgcgc ataaattgcg
    28861 gcgtaccgcg tttaataaat tgatttggct gagaaatgct ttgcttgtca gtgtcgcttg
    28921 ccggagcgcc atcaacagtt gtagtacggc tcacttgaaa tccttgctaa atatgcctgt
    28981 ggatcaggct tatacatagg gtaggaaaat cgaattgttc tgtctaatat attaataatc
    29041 tcaaataaga tgttttaaat atgaatattg aacttcgtca tctgcgttac tttgttgctg
    29101 ttgcggaaga gctgcatttt gggcgcgcgg ctgcccgcct gaatatttcg caaccgccgc
    29161 taagtcagca gattcaggca ctggagcaac aaatcggtgc ccgactgctg gcacgaacta
    29221 atcgcagtgt attgctgacg gcagcaggaa aacagtttct tgcagatagt cggcaaatcc
    29281 tgtctatggt ggatgacgct gccgctcgcg ctgaaagact gcatcagggt gaagcggggg
    29341 agttgcgtat tggttttact tcgtccgctc cttttattcg ggcggtgtcc gatacgttat
    29401 cgctgtttcg ccgtgattat cctgatgtcc atttacaaac ccgcgaaatg aacactcgcg
    29461 agcaaatcgc tccgctcatt gaaggaacgc tggatatggg attgctgcgt aacacagcgt
    29521 taccggagac gcttgaacac gcagtcatcg tccatgaacc gcttatggcg atgatcccgt
    29581 acgatcatcc cctggcaaat aatccgagtg taacactggc tgaactggct gaactggctg
    29641 aactggctga actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg
    29701 ggactgggct gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca
    29761 tcactcagga ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg
    29821 tttcaatttt gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga
    29881 ttgctgaaga ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa
    29941 gtccagctgc gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc
    30001 agcgaaaaag cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac
    30061 gtattgaagt gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta
    30121 tgcggtggtt gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg
    30181 cgcggtttat cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct
    30241 ggcgcagctg gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct
    30301 ggtgcgagag ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt
    30361 ggttgaaact gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct
    30421 tgctctgcgc gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa
    30481 agatgacttg ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca
    30541 ccagaaaaaa cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac
    30601 ggaaaatggt attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg
    30661 cgaggcgctg gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg
    30721 gacgatggca gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt
    30781 tatcgatcac aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag
    30841 cagtagtctc gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg
    30901 cgggaaccac ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca
    30961 gctgcgtctc aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc
    31021 attgtgtcag gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg
    31081 cgcgcatgta gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact
    31141 gattggctca ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat
    31201 ccgtcagcag gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc
    31261 taaccagggc acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt
    31321 gttgattcgt ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc
    31381 tcaatttggg ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat
    31441 atttgtgaag cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag
    31501 acacttctgt agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg
    31561 gaaaaagggt tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc
    31621 tgcgtaataa agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag
    31681 cagttaatcc tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca
    31741 attacaccct catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg
    31801 tagaagggac tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg
    31861 tagtgcagga acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc
    31921 atgcactgct aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg
    31981 ctaaccgaat caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa
    32041 aacttccggc accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac
    32101 tggggcaata catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg
    32161 tgtaacgtcc gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc
    32221 tgatactccc cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt
    32281 gccgccaaca gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca
    32341 gggaaccata atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc
    32401 gtcggtgtaa agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt
    32461 ttacaaagga agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt
    32521 acggtgctat agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc
    32581 aacccgccta gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga
    32641 caggcgttca ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg
    32701 ataatcagcg catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac
    32761 gcgtcgctat gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc
    32821 gggccgagag aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca
    32881 gcaagcgggg cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat
    32941 ttccattcct ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa
    33001 agaatcatcg caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca
    33061 agcgatttaa ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg
    33121 taatcggtcg gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa
    33181 cccgccgcca gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc
    33241 gctgcattga ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc
    33301 gcatgacgaa acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc
    33361 agacggcgga acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat
    33421 gggcaatgaa acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg
    33481 tgctttaaga gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gtgcgtaatc
    33541 gtcaccatcc ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa
    33601 tcaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt
    33661 agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa
    33721 ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag
    33781 ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa
    33841 tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt
    33901 cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca
    33961 aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg
    34021 gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg
    34081 caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc
    34141 gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat
    34201 gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca
    34261 ggccataaaa gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa
    34321 caagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac
    34381 ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc
    34441 tggcttgttg cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct
    34501 cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa
    34561 cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga
    34621 agaaagccag cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc
    34681 aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc
    34741 ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa
    34801 aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg
    34861 agttaaacgc tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca
    34921 tcaacagcca gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg
    34981 cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg
    35041 taaagataac cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta
    35101 gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata
    35161 ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc
    35221 tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat
    35281 atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct
    35341 ttttcagcat tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg
    35401 atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg
    35461 gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa
    35521 gcgaacacgt tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca
    35581 taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg
    35641 caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg
    35701 ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc
    35761 cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc
    35821 accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat
    35881 acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt
    35941 atcgccatga cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg
    36001 gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg
    36061 gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt
    36121 tgggcgagga tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc
    36181 tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag
    36241 tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga
    36301 ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg
    36361 gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca
    36421 tggtcgatgg catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc
    36481 atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg
    36541 tttatgccga cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg
    36601 ccgttggagt gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg
    36661 atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat
    36721 tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg
    36781 ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga
    36841 aatggctgat cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg
    36901 ccatcggtgc cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta
    36961 tagcgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag
    37021 taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc
    37081 agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc
    37141 attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa
    37201 gagttcatca gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt
    37261 ccgtacagga catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca
    37321 ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg
    37381 ccgacgcgct tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag
    37441 cgcccgcgta aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca
    37501 gactccaccc agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg
    37561 cagttaacgg tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg
    37621 accatttggg aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg
    37681 gaggttttca tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg
    37741 attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt
    37801 gtagtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc
    37861 agtggtgaag ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg
    37921 ccagacatat tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg
    37981 cgtggtgtta ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata
    38041 tcgccgttgg caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg
    38101 caggctgcct tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg
    38161 aaaccaaaca gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt
    38221 gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca
    38281 atttccgcca gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt
    38341 gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc
    38401 ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc
    38461 attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga
    38521 tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt
    38581 tcgaattttt cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag
    38641 tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt cttccagcag aatgtcggca
    38701 tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg
    38761 tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc
    38821 gcgtagttcc agatggactc cattgccaga cggatggatg gacttgaagt agttgactta
    38881 atgctactta aggtcctgat atgactgaca ttaatcatga taacactgta tatccatcca
    38941 gttctttctc tgggtctccc tatcggtatc agcaagtcta cacctcaagc cctgacacaa
    39001 ggctgacaca aatcaatcta ctcagcgtag ctaagcctca gttagtccgc agagccaatg
    39061 gtcgatatac tatccgcttc agactcaagg gacagaccac accgtttctt tcagtatcga
    39121 cccgcagcac agacaggaga gtagccacaa tgcgtcagag agagcttgca gccaccgcta
    39181 aggccttcat gttggatagg cctgaagtct cactacagga gcttacagag catctccggt
    39241 ctatggctga gcagttcctg acagacgcta gtgacgatta ctggaatgga cttgaggtcg
    39301 ctactctggt cgacgagaag tccaacctga aggagctagc cgctacgcaa gccttgagtc
    39361 tggaccagca gaagggcata aggttagcgc tggaagtcct gaccgcagca cagcagagag
    39421 tcgacactgg tgatacctct ggtctgatta agctgattga tgataataac cttactgatg
    39481 actcaactat aggcgactct acgtcaattc tgaataatga gcagggggat agaccggcag
    39541 ttttcacaca ggagcgccag tcatctgtag ttttttcaag cctagtgtcc tcgctgctgg
    39601 ctgagaaggt ccagaccctg aagacatcca gctacaagga cctctcgtcc tcactcaata
    39661 ctgtctcccg gttcctgcct gaagacatgg acctgatgtc ccgctctggg tggctggctg
    39721 tcagggactc tatgctggct tctgaggtga gaccatcgac aatcaacaag ctgctgacca
    39781 aagcgaagat gtgtctcgat tatggcctga tgaatgggca acttgagggc cgcaacccta
    39841 ttgaacggat gaagctgacc aaggatatcg actccaagcg cagagcgttc acagacgagg
    39901 agctggagag acttctggtc cgtgttgagt ctga
//
LOCUS       DAJTRW010000028        38631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRW010000028 DAJTRW010000000
VERSION     DAJTRW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..228)
                     /gene="yefM"
                     /locus_tag="OO326_001593"
     CDS             complement(<1..228)
                     /gene="yefM"
                     /locus_tag="OO326_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5966013.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKG"
     gene            511..552
                     /gene="hisL"
                     /locus_tag="OO326_001594"
     CDS             511..552
                     /gene="hisL"
                     /locus_tag="OO326_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5966014.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    528..651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            698..1597
                     /gene="hisG"
                     /locus_tag="OO326_001595"
     CDS             698..1597
                     /gene="hisG"
                     /locus_tag="OO326_001595"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5966015.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1603..2907
                     /gene="hisD"
                     /locus_tag="OO326_001596"
     CDS             1603..2907
                     /gene="hisD"
                     /locus_tag="OO326_001596"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5966016.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2904..3974
                     /gene="hisC"
                     /locus_tag="OO326_001597"
     CDS             2904..3974
                     /gene="hisC"
                     /locus_tag="OO326_001597"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5966017.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3974..5041
                     /gene="hisB"
                     /locus_tag="OO326_001598"
     CDS             3974..5041
                     /gene="hisB"
                     /locus_tag="OO326_001598"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5966018.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5041..5631
                     /gene="hisH"
                     /locus_tag="OO326_001599"
     CDS             5041..5631
                     /gene="hisH"
                     /locus_tag="OO326_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5966019.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5631..6368
                     /gene="hisA"
                     /locus_tag="OO326_001600"
     CDS             5631..6368
                     /gene="hisA"
                     /locus_tag="OO326_001600"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5966020.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6350..7126
                     /gene="hisF"
                     /locus_tag="OO326_001601"
     CDS             6350..7126
                     /gene="hisF"
                     /locus_tag="OO326_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5966021.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7120..7731
                     /locus_tag="OO326_001602"
     CDS             7120..7731
                     /locus_tag="OO326_001602"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5966022.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7917..8894)
                     /gene="wzzB"
                     /locus_tag="OO326_001603"
     CDS             complement(7917..8894)
                     /gene="wzzB"
                     /locus_tag="OO326_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5966023.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9040..10160)
                     /gene="ugd"
                     /locus_tag="OO326_001604"
                     /pseudo
     CDS             complement(9040..10160)
                     /gene="ugd"
                     /locus_tag="OO326_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10410..11816)
                     /gene="gndA"
                     /locus_tag="OO326_001605"
     CDS             complement(10410..11816)
                     /gene="gndA"
                     /locus_tag="OO326_001605"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5966024.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11925..12284)
                     /locus_tag="OO326_001606"
     CDS             complement(11925..12284)
                     /locus_tag="OO326_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966025.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12281..12787)
                     /locus_tag="OO326_001607"
     CDS             complement(12281..12787)
                     /locus_tag="OO326_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966026.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12933..13496)
                     /locus_tag="OO326_001608"
     CDS             complement(12933..13496)
                     /locus_tag="OO326_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966027.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13703..14641)
                     /locus_tag="OO326_001609"
                     /pseudo
     CDS             complement(13703..14641)
                     /locus_tag="OO326_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14704..15078)
                     /locus_tag="OO326_001610"
     CDS             complement(14704..15078)
                     /locus_tag="OO326_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5966028.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15082..16230)
                     /gene="wzy"
                     /locus_tag="OO326_001611"
     CDS             complement(15082..16230)
                     /gene="wzy"
                     /locus_tag="OO326_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5966029.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16250..17155)
                     /gene="rfbG"
                     /locus_tag="OO326_001612"
     CDS             complement(16250..17155)
                     /gene="rfbG"
                     /locus_tag="OO326_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5966030.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17148..18038)
                     /locus_tag="OO326_001613"
     CDS             complement(17148..18038)
                     /locus_tag="OO326_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5966031.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18068..19324)
                     /gene="wzx"
                     /locus_tag="OO326_001614"
     CDS             complement(18068..19324)
                     /gene="wzx"
                     /locus_tag="OO326_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5966032.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19340..19885)
                     /gene="rfbC"
                     /locus_tag="OO326_001615"
     CDS             complement(19340..19885)
                     /gene="rfbC"
                     /locus_tag="OO326_001615"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5966033.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19890..20768)
                     /gene="rfbA"
                     /locus_tag="OO326_001616"
     CDS             complement(19890..20768)
                     /gene="rfbA"
                     /locus_tag="OO326_001616"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5966034.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20827..21726)
                     /gene="rfbD"
                     /locus_tag="OO326_001617"
     CDS             complement(20827..21726)
                     /gene="rfbD"
                     /locus_tag="OO326_001617"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5966035.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21726..22811)
                     /gene="rfbB"
                     /locus_tag="OO326_001618"
     CDS             complement(21726..22811)
                     /gene="rfbB"
                     /locus_tag="OO326_001618"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5966036.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23184..24077)
                     /gene="galF"
                     /locus_tag="OO326_001619"
     CDS             complement(23184..24077)
                     /gene="galF"
                     /locus_tag="OO326_001619"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5966037.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24252..25646)
                     /gene="wcaM"
                     /locus_tag="OO326_001620"
     CDS             complement(24252..25646)
                     /gene="wcaM"
                     /locus_tag="OO326_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5966038.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25657..26877)
                     /gene="wcaL"
                     /locus_tag="OO326_001621"
     CDS             complement(25657..26877)
                     /gene="wcaL"
                     /locus_tag="OO326_001621"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5966039.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26874..28154)
                     /gene="wcaK"
                     /locus_tag="OO326_001622"
     CDS             complement(26874..28154)
                     /gene="wcaK"
                     /locus_tag="OO326_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5966040.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28334..29812)
                     /gene="wzxC"
                     /locus_tag="OO326_001623"
     CDS             complement(28334..29812)
                     /gene="wzxC"
                     /locus_tag="OO326_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5966041.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29814..31208)
                     /gene="wcaJ"
                     /locus_tag="OO326_001624"
     CDS             complement(29814..31208)
                     /gene="wcaJ"
                     /locus_tag="OO326_001624"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5966042.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31263..32633)
                     /gene="cpsG"
                     /locus_tag="OO326_001625"
                     /gene_synonym="manB"
     CDS             complement(31263..32633)
                     /gene="cpsG"
                     /locus_tag="OO326_001625"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5966043.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32826..34262)
                     /gene="cpsB"
                     /locus_tag="OO326_001626"
     CDS             complement(32826..34262)
                     /gene="cpsB"
                     /locus_tag="OO326_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5966044.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34265..35488)
                     /gene="wcaI"
                     /locus_tag="OO326_001627"
     CDS             complement(34265..35488)
                     /gene="wcaI"
                     /locus_tag="OO326_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5966045.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35485..35964)
                     /gene="gmm"
                     /locus_tag="OO326_001628"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35485..35964)
                     /gene="gmm"
                     /locus_tag="OO326_001628"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5966046.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35964..36932)
                     /gene="fcl"
                     /locus_tag="OO326_001629"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35964..36932)
                     /gene="fcl"
                     /locus_tag="OO326_001629"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5966047.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36935..38056)
                     /gene="gmd"
                     /locus_tag="OO326_001630"
     CDS             complement(36935..38056)
                     /gene="gmd"
                     /locus_tag="OO326_001630"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5966048.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38082..38630)
                     /gene="wcaF"
                     /locus_tag="OO326_001631"
     CDS             complement(38082..38630)
                     /gene="wcaF"
                     /locus_tag="OO326_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5966049.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
BASE COUNT        10320 a         9681 c         8697 g         9933 t
ORIGIN      
        1 tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga
       61 acgcagtaga taagccgtct cttccagcga attgtattct tcgagtgaca tcagaacaca
      121 agcctctcca ttctgacgag taatgaggat cggggcatga tcttcaacgg ctttcatcat
      181 tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt aaatctccta
      241 ttttgtacag ttcattgtac aatgataggt gttaattaac tatttattaa ttagtttgta
      301 gatcaagaca ttgccagcga gacgaaaaat cagactttgc tctttttggt gctgtcaagt
      361 tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca aatccatcgc
      421 cataaaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca
      481 tcagttgaat aaacattcac agagattttt atgacacgcg ttcaatttaa acaccaccat
      541 catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc
      601 atgaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt
      661 gttaacggtt cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg
      721 catagctatg cagaaatccg gccgtttaag tgatgactca cgcgaattac tggcgcgctg
      781 tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat
      841 tgatattctg cgcgtgcgtg acgatgacat tccctgtctg gtaatggacg gcgtggtaga
      901 ccttgggatt atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg
      961 tgaagatccg cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct
     1021 ggcaacgccg gttgatgaag cctgggacgg tccgctctcc ttaaacggta aacgtatcgc
     1081 cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggtatct cttttaaatc
     1141 ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc gggctggcgg atgcgatttg
     1201 cgatctggtt tccaccggtg ccacgctgga agctaacggc ctgcgcgaag tcgaagttat
     1261 ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa atggaagaat ctaaacagca
     1321 actgatcgac aagctactga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata
     1381 catcatgatg cacgcaccga ccgaacgcct ggatgaagtc atcgccctgc tgccaggtgc
     1441 cgaacgccca accattctgc cgctggcggg tgatcaacag cgcgtagcga tgcacatggt
     1501 gagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcactgg gtgccagttc
     1561 aatcctggtc ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc
     1621 attgactgga atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatc
     1681 tccgcttctg aaagcattac ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt
     1741 ggcgatgagg ccctgcgtga atacagcgcg aagtttgata aaaccacggt taccgcgctg
     1801 aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag
     1861 gcgatggcgg tggcagtaaa gagtattgaa accttccaca ctgcgcaaaa actgccgccg
     1921 gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg tcacgcgccc ggtggcttca
     1981 gtgggtttgt atattccggg cggctccgcc ccgctcttct caacggtatt aatgctggca
     2041 actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc
     2101 gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg acgtgtttaa cgtcggcggc
     2161 gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc
     2221 ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac
     2281 ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc
     2341 gctacgccgg attttgtggc ttctgatttg ctttctcagg ctgaacacgg ccctgactca
     2401 caggtaattt tactgacccc cgatgctgat atggcgcgtc gcgttgccga ggccgtcgaa
     2461 cgccaactgg cagaactgcc gcgtgccgaa accacccgcc aggcactgaa cgccagccgc
     2521 ctgatcgtga ctaaagattt agcgcagtgc gtggagatct ccaaccagta cggcccggag
     2581 cacctgatca ttcagacccg caacgcccgc gaactggtcg atggcatcac cagcgccggt
     2641 tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg actacgcctc cggcaccaac
     2701 cacgtgctgc cgacttacgg ttacaccgcc acctgttcca gcctcgggct ggcagatttc
     2761 cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc
     2821 attgaaacac tggccgccgc cgagcgcctg accgcccaca aaaatgccgt tactttgcgt
     2881 gttaacgtcc ttaaggagca agcatgagca ccgtgactat tactgattta gcgcgtgaaa
     2941 acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct
     3001 ggctgaacgc caacgaatat cccacagccg tggagtttaa gcttactcag caaacgctca
     3061 accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg
     3121 taaaaccgga acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctgctgatcc
     3181 gcgctttttg cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt
     3241 acagcgtcag cgctgaaacc attggtgtcg aatgccgcac agtgccgacg ctggaaaact
     3301 ggcaactgga cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt
     3361 gcagccccaa caaccccacc gggcaactga tcaatccgca ggattttcgc accctgctgg
     3421 agctaacccg cggtaaggcg attgtggttg ccgatgaagc ttatatcgag ttttgcccgc
     3481 aggcatcgtt agctggctgg ctggcggaat atccgcacct ggcgattttg cgtacccttt
     3541 cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca
     3601 tcaacctgct gatgaaagtg atccccccct acccgctctc gacgccggtt gccgacattg
     3661 ccgcacaggc gttaagcccg cagggaatcg tcgccatgag cgaacgggtg gcgcaaatta
     3721 ttacagaacg cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt
     3781 tcgactctga aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat
     3841 ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct
     3901 gcctgcgaat taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg
     3961 cggagcaagt ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag
     4021 cgaaccgccg agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt
     4081 gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag ctggtgatga tcactaatca
     4141 ggatggtctg ggaacacaaa gtttcccgca agcggatttt gatggcccgc acaacctgat
     4201 gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gtccgcacct
     4261 gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagg gttatctggc
     4321 tgagcaagcg atggatcgtg ccaacagtta tgtaattggc gatcgcgcga ccgacattca
     4381 actggcggaa aacatgggca ttaatggttt acgctacgac cgcgaaaccc tgaactggcc
     4441 aatgattggc gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa
     4501 agagacgcag attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca agattaacac
     4561 cggcgttggc ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgcat
     4621 ggaaatcaat gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg
     4681 cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac aaacgcggta tttgccgctt
     4741 tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc gcactggata tctctggtcg
     4801 cccgcacctg gaatataaag ccgagtttac ctaccagcgt gtgggcgatc tcagcactga
     4861 aatgatcgaa cacttcttcc gttcgctctc ttacaccatg ggcgtgacac tacacctgaa
     4921 aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg ttcaaagcct ttggtcgtac
     4981 cctgcgccag gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag gagtgctgta
     5041 atgaacgtgg tgatccttga tactggctgc gccaacctga actctgtgaa gtctgccatt
     5101 gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg acattgtgtt gctggccgat
     5161 aaactgtttt tacccggcgt cggcaccgca caagcagcga tggatcaggt gcgtgagcgc
     5221 gagctgtttg atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg
     5281 caactcctgg gacgacgcag cgaagagagc aatggcgtcg acttgctggg catcatcgac
     5341 gaagacgtgc cgaaaatgac cgactttggt ctgccactgc cacatatggg ctggaaccgc
     5401 gtttatccgc aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac
     5461 tttgttcaca gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc
     5521 gaaccgttca ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag
     5581 cgttctggtg ccgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc
     5641 cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat tacggcaaac
     5701 aacgcgatta cggtaacgac ccgctgccgc gcttacagga ttacgctgcg caaggtgccg
     5761 aagtgttgca cctggtggat ctgaccgggg caaaagatcc ggctaagcgt caaatcccgc
     5821 tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca ggttggcggc ggtgttcgta
     5881 gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc gcgcgtggtg gtcggttcca
     5941 ccgcagtgaa atcaccggac gtagtgaaag gctggtttga acgtttcggt gccgatgcgc
     6001 tggtgctggc actggatgtc cgtattgacg agcaaggcaa caagcaagtg gcagtcagcg
     6061 gctggcaaga gaactcgggc gtttcactgg aacaactggt ggaaacctat ctgcccgtcg
     6121 gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg cacgctggcg ggatctaacg
     6181 tctctttata tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag tcctccggcg
     6241 gcattggcga cattaacgat gttgctgccc tacgtggcac tggcgtgcgc ggcgtaatag
     6301 ttggtcgtgc attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa
     6361 acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg
     6421 caaccatgaa atcattggcg atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc
     6481 agatgaactg gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag
     6541 ctgggtatct cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg gtgggattaa
     6601 gtctctggaa gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc
     6661 ccctgcgctg gcagacccaa cattaattac tcgcctggcc gatcgctttg gcgtgcagtg
     6721 tattgtggtt ggtattgata cctggtacga cgccgaaacc ggtaaatatc atgtaaatca
     6781 atataccggc gatgaaagcc gcacccgcgt cactcaatgg gaaacgctcg actgggtaga
     6841 ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt
     6901 gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt gaagtttgcc acgtcccgct
     6961 gattgcctcc ggtggcgcgg gcaccatgga acacttcctg gaagccttcc gcgatgccga
     7021 cgttgacggt gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggcgaatt
     7081 aaaagcgtac ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt
     7141 cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg tgattgtgca acacgcggta
     7201 tctggcgaag tgttaatgct gggctacatg aacccggaag ccttagacaa aaccatcgaa
     7261 agcggtaaag tcaccttctt ctcgcgcact aaacagcgac tgtggatcaa aggcgaaacc
     7321 tcgggcaatt ttctcaatgt ggtaagtatt gccccggact gcgacaacga cacattactg
     7381 gtgctggcga atcccatcgg gccaacctgc cacaaaggca ccagtagctg cttcggcaac
     7441 accgctcacc agtggctgtt cctgtatcaa ctggaacaac tgctcgccga acgtaaatat
     7501 gccgacccgg aaacctccta caccgccaaa ctgtatgcca gcggcactaa acgcattgcg
     7561 cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag caacggtaca tgatcgcttt
     7621 gagctgacca acgaggcatc tgacttgatg tatcacctgc tggtgctgtt gcaggatcag
     7681 gatctggatt taacgacagt aattgagaac ctgcataaac ggcatcagtg agttacgggc
     7741 tgagcaggtg tgatgttgtt gtcacacccg gagccattgc ccgatgcgca aacttatcgt
     7801 gcctacacat tatctcaaca cacgtaggcc ggataagatg cgccagcatc gcatccggca
     7861 aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct taaatgcaca ataatattac
     7921 ttcgcgttgt aattacgcag agcgttacgc cccaacacaa tccccgcgcc aaccatgccg
     7981 cccagaagca ctgccagaat caaggtgatt gcctttttcg gactatcgcg acgaataggt
     8041 aacgtcggtt tcatcacata gcggtaagca tgaatatcaa gatcatcaaa ctttaatttt
     8101 tcaatatcca acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacga
     8161 gtcgcttcat gcttaatcat cgactccagc gcttcgctcc ctagaaggaa caacgtatct
     8221 tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac
     8281 tgcaacgctt cctgaatctg acggatacgc agatctttct gctcctgcgc gaccacttcc
     8341 tgggttctta aagagtcctg caagtttttc cgtcccagag caatgttgtc cttgagatcc
     8401 ttttctagct cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt
     8461 tgtgcgccct ctgcagtttg cccaacataa gaaacagtca atggtaattg ctggttctta
     8521 acagaaggtt cgatggtaag tttttctggc tcttcctgat tatccagcgt ttctgctaat
     8581 gcagagaagg cagaactgaa gcgaccaatt aacgtctcct gcaaatccga tactttcggt
     8641 gcagcctgac catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg
     8701 ggctgagtga taattgctgt tgacgtccat ttctccttcg ctactgccaa ataaccaata
     8761 gccagggcaa tagccacaat gacggaaatg ataattgtca tcttgccacg ccacaactgc
     8821 actagtaaat caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta
     8881 ttttctactc tcatcattac cctaactgac ggaaatttcc aagtccgcat agtttagcga
     8941 taatccccgt gaatgctata ggataagtga taaaaataat gaattattgc ggaggtaaca
     9001 aaagacaatc agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc
     9061 gcgggtgtag accttatctg ccacatcctt aagctcttct gccatacggt tggagataat
     9121 gacgtcggct tgttgtttga aggtggcgag atcacgttcc aggcgagagt tgaagaatga
     9181 gtcttctttc atcaccggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt
     9241 cataatcccc tgaatagaag acgcacggaa gttatctgac ccgctcttca taatcagacg
     9301 ataaataccc accacttgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca
     9361 gactggtagt tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca
     9421 aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc
     9481 tggcgagtat tcagacctaa actttctgca taactatcca gttcattaaa gtatgctacg
     9541 cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt ctgcttcagt ggagtcggta
     9601 aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct
     9661 gcgcgttctg aacgctcacc gatgacaata cgtgacggat ggagattatc gtaaagggct
     9721 ttaccttcgc ggaggaattc cggagagaaa ataatatttt cagtacgata tttcttatgc
     9781 atcgctgcgg taaaaccaac gggaaccgtt gatttgatga ccataaccgc ataaggattt
     9841 atctcaacta cgtctttaat tactgattct acactggatg tattgaaata attagtttta
     9901 ggatcatagt cggttggagt ggcgatgatg acataatcag catcccggta ggcttcattt
     9961 ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta
    10021 tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat
    10081 gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac atagccagta
    10141 ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt
    10201 ttttagcatc gtgataagat gctaaaagtt ttaacgctac gttgtcagat ggtcaacgca
    10261 acgaaaaaca attcagattt tctttataag aatagctgat gtttattaag ttaatattaa
    10321 gcagtgaaat ttagtctata agatatttgg caaaaaaaag cccggtgcaa tatacgccgg
    10381 gcctcaattt tattgttggt taaatcagat taatccagcc attcagtatg gaacacacct
    10441 tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt cacgctgtgc ctggatcagg
    10501 ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc
    10561 ggaaccggga taccgttctg tactgcataa gcgacgacat cgcgcagcgc ctgctggtag
    10621 tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt tagcgatctg cggattttcg
    10681 gcataagcat cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa
    10741 atcttcgcga tttcgccgta gttcagatcc caattgtatt cttcagacgc agcacgcagc
    10801 tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcact gcgaactttt
    10861 tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag aactttagat
    10921 gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc
    10981 agcgacagcg gttcgccgag atccagcgcg ctctggctgg tccatttacc ggtgcctttg
    11041 tttgctgctt catccagaat cacatcaacc aggtagttac cgtcttcatc ttttttggtg
    11101 aagatatctt tagtgatgtc gatcaggtag ctgctaagtt caccgttatt ccactcggtg
    11161 aaagtctggg ccagttcttc attagataga ttcaggccac ctttgagcag agaatacgct
    11221 tcagcaatga gctgcatatc tccgtattca ataccattgt gaaccatctt cacataatgg
    11281 cctgcgccgt cggcaccaat ataagtaacg catggctcgc cgtcttctgc cacggcagcg
    11341 attttagtca ggataggcgc aaccagctca taagcctctt tctgcccgcc aggcataata
    11401 gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac cggtaccgat gaagttaaag
    11461 ccctctgctg aaagctcacg attacgacga atggtgtcct ggaagaaggt attaccacca
    11521 tcaatgatga tgtcaccttt atcgagatat ggtttcaggg aatcaatcgc agcatccgtg
    11581 cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac
    11641 tctttcaccg tatagtaagg agccagtttc ttgcctggat tttcagcaat cacttcttcc
    11701 gtcttttcgc gggagcggtt gaaaatagag acggtataac cacgactttc gatgttgagc
    11761 gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt tgacattgtt
    11821 tactcctgtc ttctatatta ttaaccgtct attaaccgtt atattattaa ccgtcgtgat
    11881 tttcatcttc taatcaagga atacatatgg ttacagcatg cttcttactt tatagcgata
    11941 agtatgccat tgggtgtatc cataagaact ttgaattttg gatacttttc tggattaaaa
    12001 ttagcatagc ttcgtttaga tataacaata aacgaactat taaaagattt catcttctct
    12061 attaaatcat cctgtgggat tacctcacac tcgggattac taatagtcca atcactatat
    12121 cttgctaatc caaagacgtc accaattgcc atccctttat agaagtaaat accatcctcc
    12181 aagcctaatt ggataagatt atctccatat aactcactat attcacttgc tttcttcatc
    12241 agttcatagc tatagcgact ttctaagcgt gaattaaaat tcaagtattc agaataaaaa
    12301 tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat aatacacaca aggataaaat
    12361 atcgtcccaa cttgtaattt gtaatgttga tatcttttat taaacttgca attgagatcg
    12421 caacctgcaa tactgacaat gggtaaatgg gtgataaata cctgtcacct tgcgtaacag
    12481 aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa tacccaaagt attttattat
    12541 tcctaagtgt aaagacagac agtatagcat aaaaaacaac atcactgcca actttattaa
    12601 ttgcttgttg caaatctaat gtggcaggac ttataccatg tcgagcttgt tctgctgttt
    12661 gaacttgtag atcaatttca ttccataggt agtgaccaaa tatatttcct ccagcaggac
    12721 taaaaggatc accagagatc caaaaactac ggacatacca ataaatgcca ccaagaattg
    12781 ttgccaagct aaaaataaga aatgtctgta tgtttctctt tttcgctatc aatataacaa
    12841 atatgatggc tccatatatg gcaccaaata gttttacgcc acaagcaatt ccaataaaaa
    12901 aaccacataa aaaaattaat tcattttgtg ttttattgtt ttctgagcga atatatataa
    12961 gaatactggc tgcagcaaaa caaagatagc gttaaatcaa tatatgcata accaagatga
    13021 tctttaagtg gattgatagt aaatagtagg attgtagcaa taatgcctgg ataataagtt
    13081 ttcgtaattt cccatgaaca gccaacaagt ccacaggcta ttacaaaaaa acagatattt
    13141 gctaaaaact gagctaacct tacatctcct aactgtaaac ctaaagccat taaaagatct
    13201 gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa caacaattgc atggtgctca
    13261 actaatgaac gagccaaagg taagtgatac atagtatcat cccaatcacc gggagctcgc
    13321 agggacgata acaatgtaac cacaaaaatc aaaacaagaa ataaaataac aaacagcgta
    13381 ctgaactcaa tattatttat tgccagccat ctaaattgct gacgtttaat aatgcttaaa
    13441 acccaacaca ccgtaaaaat taaaaagata gcaatcttat taaattgccc gaccatgccc
    13501 agaaagtaaa ttatcaaaat atttaaagac atccctatgc ataggcaata aaaaataaag
    13561 aaaatttgtt aagtcctgcc accccctcct taaaaaaaga tgacaataca atattaccaa
    13621 tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac atacaaatac aacggcaatt
    13681 caatcatagt aaaatactct atttacatta tcagtttata gtttttttca gaaagataaa
    13741 ataatgcctt catttcacgt ctagttgttg tatttgaatg caaaataatg ccacatacaa
    13801 atgccattat tgacgataac atgatggagg cagttaaaaa ggcagtcgga aatcttctca
    13861 ccaaacctgt atcaatataa tcaacaatga tgggcaatcc taaaattata gacaaagcag
    13921 caagtaaaac gccgataata ctaaaaaagt atagtggccg ttcaagcgag taaagtttaa
    13981 ttattgtttt cagaatcttg aacccatcac tccaagttga tagtttgctc acactccctt
    14041 cagatcgctc gccatatttt gtattaacct caccatactt catacgtaat tctaaagcat
    14101 gaattgtaag ctcagtttcg atttcaaatc cccttgatag acatggaaaa gatttaacat
    14161 agcgtctgga aaatacacga taccccgaca acatatcagt aaaattccct ttaaagatag
    14221 tctgaacagt accagtcaac aatttatttc ctcagcgatg cccttttctg tatgtatttt
    14281 gatctccatt ctcttgacga catcctacaa ccatatccaa ttgatttttt atgagatggt
    14341 taatcatttc tggagcagaa cttgcatcat aagtgtcatc tccatcaacc attagataaa
    14401 tatccgcatc tacatctgag aacatacgtc ttacgacatt ccccttgcct ttcagaggaa
    14461 cagagtgaac tgttgctcca gcatcctctg ccacttgtgc tgtactgtca gtcgaattat
    14521 tatcaaatac atatatagaa gcagttggaa tattcgtttt gaaatcattt atgacttttg
    14581 ctattgcctt ctgctcatta taacaaggaa taattacagc gatattaaaa gagtgataca
    14641 taagcccctc atataaacta tttagctaat gtgcaagtaa ttgtgttttt aacaaatcgt
    14701 ttactaacga aaaatgaaat attttgatgc gcaatatcct aaaaccaaac taaaaagaga
    14761 aaaggtgaac aatgtaaata ttggagataa agctaagaga tcaaataaaa cgccgaataa
    14821 ataactcatc gcccccataa aaaatataaa tataaaatat ctagccttgg ttgcagatac
    14881 tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt gctgcacata taaaaccaac
    14941 aatgttactt cggccttgac caaaagctaa attattataa cacgcataaa atacccccca
    15001 atgtaaagca gtattgacaa ttccaattaa aaaataacta aaaaaagaac tggtcaataa
    15061 cttccctatt tttaacatcc tttattttgc tccagaagtg aggttattac taatttggat
    15121 attttctata gaaaataccc ataaaactac aacgcctata ataaaaattc ccatgggtgt
    15181 aaaaagaaat ggattagtta tagaaacggt aatactgaaa aaaagtatta acgacatcat
    15241 taatgatgtt tttgtactat ttaaaaaata gcctttcaaa aagataagca acataggaac
    15301 taatgatata aatacaccaa taacgcctgt tttctgaagt atctcaagag gtactatttc
    15361 aatccttaat cgatctaata taaatgaacc aaatcctctt ccaaacaaga acgtcgctaa
    15421 atcaacattt ttataataaa aatataaatc attaaatcta acagagtcag aatcttcact
    15481 gcgtgatccg accatgtaaa gcataattat aaatagaacg ctgcccaata ttattataat
    15541 agtatttaaa tatagcttat gtaatttaaa actggctaat aacagtgata atgttgtaaa
    15601 aagtaataca cctcttgatt ctgtcaataa tatcgcaagc actattatga gacattttaa
    15661 atattttttg tcgacaaaac tgataatcgc accgacacca aaaaagtaga aggatttgga
    15721 aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc aatgataagt gttcataaat
    15781 taaattaaaa tttaacaaac cgaaattcaa caaaattatg tatgaaatat ataacagtga
    15841 tccatataat gcaaatgcat taactatttt aataaaaatc tcacatgaaa atacttttaa
    15901 agtatatctt agtgtaaata gatatataag tgacagcata aaaaggtatg gttgcacatc
    15961 aaacagtata tcatttattt tattaccagc taataagccg acaaagaagc cataaaaagg
    16021 aaataaaagc aaccatataa aaagactgac acatttttgg gtgattgaaa aattgaatgt
    16081 aactaaatta aaaattaaaa gtataaaact aaatacaaat aataaatatc gcaaagggaa
    16141 tattcccaat ggctccagta atcttccacc accaccaata atcagttcaa tacataaaaa
    16201 tgttataaaa attttattta tattattcat attcgtaagg tgatgttttt taaaatatgt
    16261 ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt aacttccaaa
    16321 aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta ttcacaaagc
    16381 tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac ttgtttctca
    16441 ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact tggttgtttt
    16501 catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa tttctcgtcc
    16561 gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca tttttattat
    16621 ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat gtacaccccg
    16681 gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta tcaactaaat
    16741 tttttcgccc tgatggataa tatgcaatta cattgctgga ataacaattc gcattattaa
    16801 ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg tcctgatcac
    16861 taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta taaaaatttt
    16921 tacctgcccc cccaaactta ttgacgcttg gaagtatatt tataattgag ggatatgata
    16981 attgaaattc ttcacaaata tttaatgtat tatcagttga taaatcatct gatataaaaa
    17041 ttgagatgtc tacatctttt tgttcaataa tactttttaa ttgttcctca atccagcatt
    17101 cgccattata cgtagccata agtacagcta ctcttttttg atcagaacta tgcattataa
    17161 cgaccgcccc cagtaattcc tcttattcca tccagcgtag ccaaaatatg ttttttcata
    17221 tattcaattt tatttttttg agttgcgata attaaagtcg tatgaattaa agaaaaaaca
    17281 acttccctaa tagagagtaa aagaggcaca tgattttttc taagcaaaag aaatgcattt
    17341 cttactcgat aataacgacg tactggcgag tgaattggta tgttaatccc acagattttt
    17401 actgacttat ccccaatagc atgaaccatt tctatatcag gaatgatatg aactaatatg
    17461 ccatagttta agcatctaag acaccattct gtatcgacat aatcaataaa aagttcctca
    17521 tccatcattc caacaatctc aaaaacttct tttgaaacca tggttccgga tgagattgtt
    17581 actgaacttt taaacggtgt ctgtccttca gtgacatgaa ttttctctcg aagaccattt
    17641 tttttgatat tacaaatagg ataataatgt gacttatctc tatcaataaa aactgggcca
    17701 gctatttttg cattttcatt atccatatag caggaaagtt ttttgataaa ctcattgcga
    17761 atacggctat cttgatcaaa aaaaattatc ttctctgccc ctttttcaag agaatatcga
    17821 atcccaatat tttgtgcttt tgctatacct tcattcctac caagacaaat taaatgaact
    17881 ttttcaatgt ctgctaattt atctttcaag gtgaaattag tcctattatc gactactaca
    17941 acgtaacaat tttgctcctt tagttcagtg atcagtgcat tcagattttt aagttcggga
    18001 ttatatgtca caatgacagc gtaaatatta ctattcatta tccaagtgac tcagtaattg
    18061 gttaatttta gttttcatat acagaacaca ttctagtata aagccattgt aataacagag
    18121 catctaaaat tacacgtatg ctccaggata ttgcggcacc cagtaaccca tatttttcga
    18181 ccataaaata caaaagggct aaataaggaa ccaattcagc acaatgaatc aaagcagtaa
    18241 ttttagattt tcctaatgcc tggattgcag agaatggtat cattgccagc gcattgaaga
    18301 aaaaaccgat caataacaca tttaatatat taaaggaatt aatttgataa ttctcaccaa
    18361 accatatttt caatacaagc cctgaatata acaagcctat gattatcacc ggcaaacaga
    18421 ttagaaacat taaaagcaat gatttattta cattacgttt aaattcttta aagtctttga
    18481 tgttacttaa ccttggaaac actgccctcc cgattgctgc aggtattatt cctaatttta
    18541 gaataacctc cgctggtgca gaataaaatg caactttatc tgcccccata atatttgaca
    18601 ctataaatcg atcaaaatac accatgaccg gacttatgat attacttact gtcatccaac
    18661 cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa atgaaccccc gaaattttaa
    18721 tatcattcct gacataatag gcagagacta aaatcgaaat aactcttgca aatattaaac
    18781 cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa tatggccgga attcccgcga
    18841 tgcaagagct tgatatagat ttttgaatat ttacaatgcc aaatttttca tccccctcaa
    18901 gaatggctga ccataattga tttagaataa ataatggaat gcaaatagcc aacagtttaa
    18961 atgctagttg tatatcacta tgttcaacac cagaaatttt tagataatta acaattccat
    19021 cagagaaaat caataataaa aaagcaccaa agcatgaaaa tagcaccaaa aaagatgtac
    19081 ttgttgaaat taccttggtt ctttcatgat gattatcacg atgaattgca atttctctaa
    19141 taacagagcg agtcaggcct acatcaaaaa ttccagcata cccaacaagt gcaattgcaa
    19201 ttgtataaac accgaaacct tcaggcccca aacctcgagc gagaaatcct agcgagggga
    19261 tagcaattaa agttggtatt gcatagccaa aaaggttcca gacactattt tttattatgc
    19321 tcattattac atttctttat catataaaat aatcagcatc tacaaatttt tgaccattaa
    19381 tatctttatc agaaagcaat ggattttgaa ccggccattc tatatttata cttttgtcgc
    19441 tccataaaat cccacgctct tgcatttttg aatagaaatt atttgttttg tagacaaatt
    19501 ctgcttcatc actaagaacg caaaacccat gagcgaatcc ttccggtatc cataattgac
    19561 gcttattctc cgccgacaaa tttacaccaa cccacttgcc aaatgttggt gatgatttcc
    19621 gaatatcaac agctacgtca aaaacttcac cgacaacaca gcgtaccagt tttccttgag
    19681 cataaggttc cagctgatag tgcagtccgc gtaacacacc cttagttgat ttagaatggt
    19741 tatcctgaac aaactcaacc tttcgtccta cagcctcttc gaaaactttc tgattgaagc
    19801 tttccataaa aaagccccgc tcatcaccaa atacttttgg ttcaaaaatt agcacatcag
    19861 gaatttcagt tttaattacg ttcattttat taataacctt taatcatttt cagcagatac
    19921 tgaccataag catttttctt cagcggctcg gctaatacct tcacttgctc agcatcaata
    19981 aagcctttac ggtgagcaat ttcttccggg caggaaactt tcagcccctg acgctcttca
    20041 attgttgcaa tgaagttgct tgcttcaata agactttgat gtgtccccgt gtccagccat
    20101 gcataaccac gccccatcat agcgacagac aaacgtcctt ggtccatata aatacggtta
    20161 atatcggtaa tttccagttc gccacgtgca gatggtttaa ggtttttcgc catttctaca
    20221 acgtcattgt catagaaata aagcccagta accgcatagt tactttttgg ttcctgcggt
    20281 ttttcttcca gactaattgc cgtaccgtta tcatcaaatt ccaccacacc gtagcgctca
    20341 ggatcattta cgtgataggc aaaaaccgtt gcgccactct ctctgttaac agcggtatcc
    20401 attaatttcg gcaggtcgtg accatagaag atattatcac caagtaccag cgcacaatca
    20461 tcaccaccta taaattcttc accaatgata aatgcttgcg ccagaccatc cggactcggt
    20521 tgtactttat attgcagatt aagcccccac tggcttccat caccaagcaa ttgttgaaaa
    20581 cgtggtgtat cctgcggcgt actgataatc agaatatctc gaatacccgc caacataagc
    20641 gttgaaagcg gataataaat catcggttta tcataaatcg gtagcagttg tttgctcaca
    20701 gccattgtca caggataaag acgagtgccg gaaccaccag ccagaataat acctttacgc
    20761 gttttcattt caccattcct tttaattcat ctcgctctag catcatgagc gagatgcaat
    20821 aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc atacgtttca cgcccacctg
    20881 ccagtcaggc aagacaagcg caaagttctg ctgaaatttt tcggtattga ggcgagaatt
    20941 atggggacga cgtgctggtg tgggataggc cgttgtcggc acggcattaa gtttgttaag
    21001 agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc agagcggcat aatcgtgcca
    21061 ggttgttgta ccaccagcaa ccagatggta caaaccagca acttctggtt tatttgcggc
    21121 cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca gcccccgttg gtgcgccgaa
    21181 ttgatcgttt attacagcca actcttcgcg ttcttttgcc aggcgcaaca tcgttttggc
    21241 aaagttattt cctttaccag cgtataccca gctggtacgg aaaataagat gctttgcgca
    21301 atgttcttgt aacgcttttt ccccagctag cttggtttca ccgtagacat ttagcggtgc
    21361 tgttgcatcc gtttccagcc atggcgtgtc accgttgcct gggaaaacat aatcagtgga
    21421 gtagtgtata acccaggctc cgacttcatt agctgctttt gcaatcgctt cgacgcatgt
    21481 cgcgttaagt aattgcgcga aattcggttc tgattccgct ttgtctactg cagtgtgagc
    21541 agcagcatta acaataacat cggggcgaat ttttttgacg gtttcagcca caccttcggg
    21601 gttgctgaaa tctccacaat aatcagtgga gtgaacatcg agcgcaatca agttacccag
    21661 aggagccaga gcacgctgca gttcccaacc cacctgccct gttttgccga aaagcaggat
    21721 attcattact gacggccctc atagttttgt tcaatccacg actgataagt accgcttttc
    21781 acattctcaa cccaatttgt attagccaga taccattcca ccgttttacg aatcccgctc
    21841 tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa ttttatcggc atcaatggcg
    21901 taacggcggt catgccccgg gcgatcggca acataagtaa tttgctcacg ataagatttc
    21961 tctttcggta caatctcatc caacaaatca caaatagtga gcactacgtc gatgtttttc
    22021 ttttcgttat gtccaccaat gttataagtt tcacccgctt taccttcggt tacgacggta
    22081 tataacgcac gagcatgatc ctctacatac aaccagtcgc gaatttgatc ccctttacca
    22141 taaataggta atgccttacc ttccagagca ttcagaataa ccaatggaat aagtttttcc
    22201 gggaagtgat aaggaccata attgttcgag caattagtca caatggttgg taaaccataa
    22261 gtccgcttcc aggcacgtac caaatgatcg ctggaagctt tagacgcaga atacgggcta
    22321 cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg cttcgttatt atttgcttca
    22381 tccggatggg gtaagtcacc ataaacttca tcagtagaaa tatgatgaaa gcggaaactt
    22441 tttttctttt catcattcag agcagaccag taattgcgcg ccgcttctaa aagaacataa
    22501 gtccccacaa tattggtttc aataaatgcc gcagggcccg ttattgaacg gtcaacgtgg
    22561 ctctctgccg ccaggtgcat caccgcgtct ggctggtgct gtgcgaaaat acgcgacatg
    22621 gctacagcat cgcagatatc tgcatgctca aaagcataac gttcagaatc agaaacatca
    22681 gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga cattaacaac actatcttgc
    22741 gtattattta ttatgtgacg aacaacagca gaaccaataa accctgcgcc accagtaaca
    22801 agtatcttca cttttttatt ccatatagct atagagcatg ctgtgaaata gactgctcca
    22861 gatttgatta atagaggtat taatacacgc taccgcccct ggcttaacag ctaccagtgc
    22921 actgcatgca tctcaacgat gtggcgagcg ttagccactc gcgccaaatc cgaaaaattc
    22981 aaacgttaat tgtcttacca aacagccgta gaaacaagga aaatcctgga aaaatttgaa
    23041 taatatgagc tatgtaactt cttgttttta taggctttat aaaaacggca acggcaggaa
    23101 aattcaatga gcaaaactta tgccacgaat cttcactgcc gttataattt tcttatcaac
    23161 cgttacatcc ggtcagattt tcattattcg cttaacagct tctcaatacc tttacggaac
    23221 ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa acgcctgcat atagcccatt
    23281 tttttgccgc agtcgtaact gtcgccggtc atcagcattg catcaacgga ttgttttttc
    23341 gccagctcgg caatagcatc agtcagctga atacgtcccc atgcaccagg ctgagtacgt
    23401 tccagttccg gccaaatatc ggcagaaagc acatagcgac ctacggccat gatgtctgag
    23461 tccagcgtct gcggctgatc cggtttttcg ataaattcaa caatgcggct gactttaccc
    23521 tcacggtcca gcggctcttt agtctggatg acggagtatt cagagaggtc acccggcata
    23581 cgttttgcca gcacctggct gcggcccgtt tcgttgaaac gtgcaatcat ggcagcaagg
    23641 ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa cgtctggcag taccacgaca
    23701 aatgggttgt caccaatggc aggtcgcgca cacaaaatgg agtggcctaa acctaaaggt
    23761 tcgccctgac gcacgttcat aatgatcacg cccggcggac agatggactg tacttccgcc
    23821 agcagttgac gcttcacgcg ctgctcaagg agtgattcta actcataaga ggtgtcgaag
    23881 tggttttcga ccgcgttctt ggacgcgtga gttaccagga ggatttcttt gatccctgca
    23941 gccacaatct cgtcaacaat gtactgaatc attggcttgt cgacgattgg tagcatctct
    24001 ttgggtatcg ccttagtggc aggcaacata tgcatcccga gacccgctac aggaataact
    24061 gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg ttgctgaatt atagcttgtt
    24121 cgattttttt cgccagcatc aattaccctg aattgattac tgaattactt gtgatgttac
    24181 gccgcttcgt tgtggattgc agtagcattg ttcctaagta tggctccatt tttccaggaa
    24241 tggtcgcaaa tctactccct ccgttccggc aatctaaagt taatcttttc cacattaaca
    24301 atatggtgat taatcctgtc gatatcgacg gagctttgcc ctctttcatt caccgcatga
    24361 acatttgcaa gagacagcag tgtttctttt ttcgccataa agacgccgcg aacgtctttg
    24421 cgcatgtcga agttcatgct caatgctggt ccaactgagg attcctgcat cacattgata
    24481 tttcgcataa aaagatgttg cggcttgttg tgtaactcca gcgaggcacg cttcatctca
    24541 atgttagtta gcgacacaaa ggagacggca ttcccggcgg agatttggat gccgcgcaat
    24601 ttataagcaa gatgggtatt atccagttga atattattca ctcggaaatt ttgcggtatc
    24661 gagagatatt tgcctttaat taccccatag ccgattaaca tcccggcgct attaatcatt
    24721 tcaatattat caatcacgaa attgtcacaa ccgtaaatag cgactgtcgc gttatcaatg
    24781 cccgctttct tactgaaatc cggcgtgata ttgcgggcat tgatattacg aataacaaaa
    24841 tgtttaccat tttcaacatg gatcaactgc cgacaatccg atcccgtgat attcgccacg
    24901 acaaagtttt tcactgcctg gtcttccggg tagttgttat cataagtgct tcccgcaagg
    24961 cctatgccga tgccccagtt gattttgccg ttggtacagt tgatacgctc gatgacatgg
    25021 tcagatatca aaatatcact gtcgttaatt gccacgttcc attcaatggc gtcgccctgt
    25081 aagtcgctga acttacagtt ggtaatgttg gcaccgataa tctggttatg aaatccctgg
    25141 cgtaagatgg cgtaattagc gtggctgaca gtgaggttat cgatggtcag gttgcgcatg
    25201 acccgtttgt ttttgccgcc aatataaatc tgcattaccg ggccaaagcc gctcatcgcc
    25261 agccctttga tggtgcagtc agaaccacgc acatccaggg taatgttatg cataccgccg
    25321 ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa atcgccctct gccgttgccg
    25381 cgcaggcttc caaggatgtg taacgttttg ccgggaggga tgaagatgcc ggtgttgata
    25441 ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc cttcgctgaa ggcttgttta
    25501 aatgaggcga tccagtcatg tgggttgtag tcgttaatgt taacgctttg tcgggcggga
    25561 agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg agcttgccgt caggaaggtg
    25621 cgtcgggaga gtgttttaaa tggcatacgt tctcctctat aaagcctgca gcaagctggc
    25681 gagttctcga ttaatcacct gctggttaaa atcgtgttcg actttttcgc acgcacgttt
    25741 gacgaccgga gccagttcgt cggtatccag ttggctaaac gccgccagac gttgtgccag
    25801 tgcgcgagca tcgttctcag gcagcagcca gccggattta tcggcctcca ccagttcagg
    25861 aattccgcta tgcagagtag aaaccaccgg aataccgacc gccatcgctt ccattagcgc
    25921 caccggaatg ccttccatat cgccatccgc gcccgttacc gatggcaaca ggaagacatc
    25981 cgcgtcgtcg agcatctcct tcacttcatg gctcggttta aagcccggca tctctaccac
    26041 atcttccagt tgatattgtt cgatgatggt gcgcaggcgt cgttcccaca ggccaatgcc
    26101 gaggatgcga tagcgaaatg ccacgccctg ctctttcaac tgacggcagg cttcaatcgc
    26161 cacatgcagg ccttttttct cggttaagcg tgcgacagat atgatttcca gcggcgttgc
    26221 gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc acgcccatac gcgatacggc
    26281 gattttttct ctggggcagc ccattttttg cagccttccg gcccacagat cgcttatcgg
    26341 taacatcagg tcgccacggc gaaacagttg ttgatattcg ggagtgtagt ggttgagcac
    26401 ttcccgactg gagatatcaa taccgtggaa gatggtggca attttgccgc gaatgatacc
    26461 cagttcgcgt agttttgctg cggttacccc cgccgggcca aaatgagcga tgaacacatc
    26521 ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac aaaatcaggt tccgcgactc
    26581 ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt ttacgatgaa tgccacgtaa
    26641 ggtctggctg gcgcggtggc gcagtttcgc tactttgccc gcaggttcgt cctgtaacca
    26701 gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg tgggtgttct ctgtatcgcc
    26761 tttttgcaaa gcgacaatct ccacttcaaa gcccatatca ataaacgcgg taatttgatt
    26821 gaggacgaag gtttccgacg acagcgggaa tttcaataag aagaagccga ccttcatttc
    26881 acctccccga tgcgctcaag cacagactgc accatctgca ttcccgtctg acgttcgcga
    26941 ctgacggctt cgttaaatcg agcgttcagc accggaagct ggcctaaggt atccgcaacc
    27001 atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa tcgccatctc tggcagtccc
    27061 agctgctgca taatcccggc ggatttatgt tcatagttga tggcaatcgc cggagtagca
    27121 aagttcattg agataatggc ggagtgcagg cgcgtaccga cggtgagttc acaggctccg
    27181 agaattttgc ccatttccag atcgttgagt tcatccatca ctacgtggta acgggcagga
    27241 tcgctgatgt gctggcgcag attgagcgcc accatgcggt cgtctttgtt atagctgtcg
    27301 atacccgtac aggtggagag cgcaatcacc tgatagcctt catcgagaat gcgattgacc
    27361 accccggcaa aggctttttc atacgcttgt tgagtggtgc cgagacgttt gtcgaacggt
    27421 gccagttcgc gcagggtaat tgccactgtt ttctgttgtg cggcaacgtc cagccagtgt
    27481 tgaacggcat agctggcggt gaagtcttcg gtgtggtgat cgaccagcca cgcggtatcg
    27541 acgccatgtt ccacttttgc ggtggtgata tttctgcgtt tcatcaaatc aaggctgacc
    27601 gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat agttcgccag ttggttaaat
    27661 tgctcatcct ggaaggggcc gacgctgtga ccaatcataa acagcggctt tttcgccata
    27721 aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga gatcgacaaa aaacgatccg
    27781 ccgacctgaa taatggcgtc gtaacctgac agcaggcgca cgaagtcggt gaatccctgg
    27841 gcgatggcga tattgcgcag cttgccagtg tcggtgacgc gtgagagcaa tacctgatgc
    27901 tggtagcggc gacggaggac ttttttaatg cgcccgacaa cgcccgccgc gctattgtgt
    27961 tgtgtcatct gcaggaacag cggatcgccc attaccgggc ggttgagcag ccaggaagaa
    28021 ctgaccggat agcggctcat cacgtccact tcggtatgtg gattgaggat gttgatggca
    28081 tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc cgcaagtgtg gttgcccaga
    28141 ataagtaatt tcattttttc ctcataaatt tgatgccagg tgaggctgtg tttgttgtgt
    28201 tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt atcaggccta caggtcccga
    28261 gcacagaacc gtaggacgga taaggcgttt tacgccgcat ccggcaaccg ttgtcggaac
    28321 cgaaaacagc aattcacccc gcccgtaaaa gcattttcat tttttcgctg cgacaaaact
    28381 gacgcttcac ttccaccacc agcggatggc gtgacagcac aatcatcacc acaaacgcca
    28441 gcacgcccgc ggctatttgc accgccagca gcatccccag cgccagttgc cctttcagca
    28501 caatgcccag cgcataactg accaccagcg tcggcaacga gagataaaac ggcagccaca
    28561 aactcaggat gtactggcga taactggaac caagaaccgg tttaatcatc acgaagtaac
    28621 tcagaatggt gttgataatc tgcaccagca ggaaaccgag cgtgacgccg attgcgcccg
    28681 ccatctgccc accaataaca atcgccggaa taaacagaaa tgttttgaat acgttgaatt
    28741 taaagctgat atcgacccgc gctttcgcca tcagcagcga accaatcggg ttacccacgg
    28801 agcgcagcag acccacaata cacagcaatt gcagcaccgg aataatgctg ttccactttt
    28861 cgccgaatac cagcggtacg aagttattcg acaccaccat cagccccagc agcgccggaa
    28921 agttgataat ccccactatc gacagcagct tgtagaagtt aacacgcagc ttttcggtgt
    28981 cgtcctgaat tttggcaaac gccggaaaca acacgcgggt gatgattggg ttcagcttca
    29041 tcggcggtac gacggccacg ttgtacgcca ggttgtatcc ccctgccacg cccgcgccaa
    29101 gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag atagttgatg atgctgtccg
    29161 ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga cgccagcgag aagtgcagac
    29221 cggggcaata aattttgcgt ccaaagtagc caaacagcag tgttctcacc gcactattga
    29281 ccagataacc gaggatcgcg gtcatcgcca gcggccagaa atgggcgcta actaccgtaa
    29341 aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc gattttgttg aactccagct
    29401 ccttctgcat caacgcgcgg aactgttgcc cgtgggggat caccacaaac gccagcgaca
    29461 atgttttaat caacggtgcc aggtccgggt tattcagcac gtcgccgatg acatcactca
    29521 acaaaaacac cgccacacat accacaagcc ccagcccgac gttcagccag tacagcgtgg
    29581 tgagttcaag gtgactgatt tcttttcgct gaataatcga gttagcgata ccgaagtcag
    29641 aaagcgtatc tgccagcgcg ataatcacca gcgacacagt cagcaggccg aactggtggt
    29701 tgtcgataat ccgcgccagc acggtcatct gcaccagccc gaggccgatg atgatcaccg
    29761 tggcaatagc cgaccacttc gcgccgctga tggttttttc acgtaagctc atatcaatat
    29821 gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa cgattttgat atcgaaccag
    29881 acgctccatt cgcggatgta ctcaaggtcg aactcgacgc gtttttccat tttctccagc
    29941 gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc cggtaatgcc cggtttcact
    30001 ttatggcgca gcatgtagcc ttcaatgagc tggcgatact gttcgttatg cgctactgcg
    30061 tgcggacgtg gaccgacaat cgacatcccc ccggtcagca cattgataaa ctgcggcaat
    30121 tcgtccagcg aggtgcggcg cagaaagttc cccactttag tgacgcgcgg atcgttctgc
    30181 gtcgcctggg tcaccacttt gtcgttctcc atcactttca tggaacggaa cttccacact
    30241 ttgatcggct tgccatccat accatagcga gtctggcgga aaataaccgg ccctggtgaa
    30301 ctgagtttca ccgccagcgc aatacagcac agcaccgggg agatcagcag cagaataagc
    30361 gtcgccagca caatgtcttc cgcacgtttt agcaggcggt taacccccga aagcggcgtg
    30421 tcatacagcg gcaccaccgg aacgccgttc atctcttcga gacgtgaatg gagaatgttg
    30481 aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg tatccgccag ttgatggacc
    30541 agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga tatagacgtt atgaatcttg
    30601 cccgcttttg catcctcgac cagctgttgc aggttacccg cccagtcgtt agaaacgccg
    30661 cccggtttcg ggtcgtggta aacgcccacc acttcaaacc ctaaccacgg ctgattacgg
    30721 aaactctcca tcagcatttg tccggcggct aaatcccccg ctacggcgac catccgcttg
    30781 ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa tacatgaacg gcaaaccacc
    30841 agtccgatgc tggtcagccc ataccacgcc agccagattt tcagttgcgt gtcgaaatca
    30901 ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta aggtccagtt ttgtagcaac
    30961 agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc gataaaaatc ggtgatgccg
    31021 cccagcatct ggaacaccac cagcgtaatc agcgccacca acaggtgcat gtagaggaat
    31081 gacagtccgc tgacttcgca aaccagccat agcccggcaa acatgatggt gatatctgaa
    31141 aagcgttgca ccatagagat taacgatgca ttggttttcg ctcgctcgcg cttttttaga
    31201 tttgtcatcg ttgttcctgt tattagcccc ttacccgcag tggggtaagg gaagatccga
    31261 cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag cggcacatca
    31321 ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat tggaggtgcg caggttaaag
    31381 cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat ccaccgccag cgcctcacgg
    31441 ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc
    31501 tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag cgtttttcct
    31561 ttcaggcaca ccagttcggc gaccagcagc cacgggatca tgccgctgtc gcagtaagcg
    31621 aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc ttccttgcgc
    31681 atccgttctt taataaaggc gtgtccggtt ttcgacatta ccggcgtgcc gcctgcagca
    31741 gtcaccacat caatagtgtt ccaagagaga cgcggatcat ggatgatctt cgcgccggga
    31801 tttttttcga ggaacgcttc tgccagcagg ccgacaatgt agtagccctc gataaactgc
    31861 cctttttcgt caaacagaaa acagcggtca aaatcgccat caaaggcaat gcccatatcc
    31921 gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac attccggcag cagcgggtta
    31981 ggaataccgt tggggaaatt gccgtccggt gtgttgtgta ctttgattaa ttccacgggc
    32041 gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc tgcgccattc
    32101 ccggagttga tcaccagctt gagcggcgtg agatttttga cattgatata accgaacagg
    32161 tgatcaacgt aagcgtcacg caggttgatt tgctgatagc gaccgcgttt ggtttcatcg
    32221 acgggaggaa agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc ggtatcaccg
    32281 ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt tgtaatccat cggattatgg
    32341 ctggcagtaa cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct
    32401 tcggtgccgg acataccgat atccagcaca tcgacgcccg catcctgtaa accttttgcc
    32461 agcgccagtt ttaaggtttc gctggtgaga cggacatcac cgcctaacac aatggttttc
    32521 ggtttgagaa attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct
    32581 tcgcctaatt tcccgcgaat atcataggct ttaaagcagg ttaatttttt catgtcgtta
    32641 ccctttttca ggcaatagtt ggccctgacc gaaacggcca attttatttt tgtcgttatt
    32701 tacaccgcgg tttcgcattc attgcctgat gcgacgctgc cgcgtcttat caggcctaca
    32761 aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc cgacaaacag cgcctgatgc
    32821 gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa
    32881 gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcagtgc
    32941 gtcgccccca gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca
    33001 atggtgactt ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg atggtgcatc
    33061 tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc
    33121 gcgtcgatag agtcatattt gccccacgga cgatacactt cgcgatgcac ccgatgctca
    33181 tggcgaccat cggctttgat ctgctcgacc acttttttca catcctgtac cgcgttacgg
    33241 tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg
    33301 gtgaccaggc ctgattcggc atacacatag ctgttttcag ttttgtgatt aatcacatcg
    33361 ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga agaccaggaa
    33421 ccaacatcgc tccagcccgc atccatcggc accacaacag catctgccgt aggttccatg
    33481 accgcgtaat ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata
    33541 aaatcgagat ccggatcgac ggcgctcatc gctttttcgc aggcatcgag gatatccggg
    33601 cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc
    33661 cagtaatatt cgccgcttgc tacataggcc tgggcggttt ccagattcgg tttttcgaca
    33721 aactgcgcca cttcaaaggc caccgtatcc tgctcaccca cagacacttc accgcgacga
    33781 atatagccat aaccggtttc tggtagatcc ggcacaatgc caaaggtcac cagcttgccc
    33841 gcttcggcat aaggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc
    33901 acatgatccg ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc
    33961 gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc ctgccggttc gagaataatg
    34021 ttctcggtga gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca
    34081 ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc
    34141 agcatggtga gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc
    34201 ggccataaac ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc
    34261 ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc
    34321 gttcggcata ttcacgtgcc accgtgttat gtttgggcag caggagcgcc tgacgaatcc
    34381 ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaataccc ggaaaggttt
    34441 cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg
    34501 ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca
    34561 ccagatggca atcgcccatc ttcagcagtg caggtaaagc gtcatacgat tgcagcggga
    34621 aaaattgcat gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc
    34681 cgccctgccc gacaatggca aaaatcagcg gttcatcgcg caggagatcg gcagcttcaa
    34741 taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt
    34801 tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca acatgctgaa
    34861 aacgggcaat ttccgaccag ttggggaaga agatgacgtt atccgccgcc acgccttttt
    34921 cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac gttatcgacg ttatgcagtc
    34981 cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct tttccggcaa
    35041 ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac
    35101 cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc actacgccaa
    35161 taatgcgatc cggcttccag cgacgttgcg ccatcagtgg aaagaaactg ctgacggcaa
    35221 aactgccgag atgcaacaag cgtttcaggg tgctcggctg ttttggcacg tacagcgggc
    35281 agcgccacac cgtggcggcc ccttcttctc gtttgtagcg ccaggcggaa tagttctcac
    35341 ccacctgcca ttgcgggtag taaggcggtg cggtaatgac ccgcacctca tgaccttgtg
    35401 ccgccaacca ttccaccatc tcgccggtgt atttgccgat gccggttaac tccggcgagt
    35461 agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag
    35521 aaaataggct cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag
    35581 ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc tcttcttccg ctactctgaa
    35641 gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata
    35701 aaaatgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc
    35761 cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca cgcgccctcc
    35821 cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcgcc
    35881 gcgactgttc tcgacaataa agtcgagaga gacaagcggt gtggagcgca ctacagtggc
    35941 aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc ggtcttgatt ctcaaggaac
    36001 cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga
    36061 tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa
    36121 accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg
    36181 cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gttgggtatt ctccagccag
    36241 acttcatgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg
    36301 aattcacgca tcggtgtacc gctgccccat accaccacgt ccggcgcatt ctgtgccgtc
    36361 gcctcgtgga agcgacgcag caatgctggt atcacatggg agttactcgg gtggaagttg
    36421 tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt aatcgcgtcc atactggcgg
    36481 ttgtaagatt cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc
    36541 ggctccagcg tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg
    36601 tagatgcagg acgatccgag aaacatcagt ttgttcacgt cgttctgatg cgcggcgtga
    36661 atgatgttgc tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg
    36721 gcaacaatgc cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca
    36781 aagaaatcat gcacggcgcg gctgtccagc aggttcagtt catcgcgggt acgtaatacc
    36841 agctccacat caccgcgctg ttcgagctgc cgcttgatgg cagaaccgac catcccacaa
    36901 tggccagcga taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt
    36961 cgtagccgtg agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca
    37021 tttcagacac catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt
    37081 tggtcgggtc gccgagcagc gtttcaactt ctgccggacg gaagtaacgc ggatcaacgg
    37141 cgataatcac atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa
    37201 tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgctgccg
    37261 ccatttccac aaactgacgt acggagtact gaacgccggt cgcgataacg aaatcttccg
    37321 gctgttcctg ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt
    37381 cacgcaggga atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg
    37441 cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt
    37501 tgaagagaat tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc
    37561 agtaggcgta cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt
    37621 tctgcggaat ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag
    37681 ttttcttttc cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt
    37741 cgacgtcagc ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccta
    37801 ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac
    37861 tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt
    37921 gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac
    37981 ctttttccag cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga
    38041 gagcgacttt tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt
    38101 tcgcgtatca ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa
    38161 acactgcttc gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag
    38221 acatcggttg ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa
    38281 tgttgacttg catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa
    38341 tgtgcgccaa tggttatttc accgagggta tataaattga cgtcatcgcc aacccacgcg
    38401 taatcaccta aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg
    38461 ttttttccta tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact
    38521 tgtggcgacc aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg
    38581 ttgccgcccc ggaacccttt cggcaccgag aaaccgctta aatcttgcat t
//
LOCUS       DAJTRW010000029        38110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRW010000029 DAJTRW010000000
VERSION     DAJTRW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(185..1294)
                     /gene="anmK"
                     /locus_tag="OO326_001632"
     CDS             complement(185..1294)
                     /gene="anmK"
                     /locus_tag="OO326_001632"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5966050.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1568..2035
                     /gene="slyB"
                     /locus_tag="OO326_001633"
     CDS             1568..2035
                     /gene="slyB"
                     /locus_tag="OO326_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5966051.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2082..2516)
                     /gene="slyA"
                     /locus_tag="OO326_001634"
     CDS             complement(2082..2516)
                     /gene="slyA"
                     /locus_tag="OO326_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5966052.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2717..2953
                     /locus_tag="OO326_001635"
     CDS             2717..2953
                     /locus_tag="OO326_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5966053.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2956..3813
                     /locus_tag="OO326_001636"
     CDS             2956..3813
                     /locus_tag="OO326_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5966054.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3813..5825
                     /locus_tag="OO326_001637"
                     /pseudo
     CDS             3813..5825
                     /locus_tag="OO326_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5826..6347)
                     /gene="sodC"
                     /locus_tag="OO326_001638"
     CDS             complement(5826..6347)
                     /gene="sodC"
                     /locus_tag="OO326_001638"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5966055.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6428..7324)
                     /locus_tag="OO326_001639"
     CDS             complement(6428..7324)
                     /locus_tag="OO326_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5966056.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7373..7612)
                     /locus_tag="OO326_001640"
     CDS             complement(7373..7612)
                     /locus_tag="OO326_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5966057.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7715..8314
                     /gene="nemR"
                     /locus_tag="OO326_001641"
     CDS             7715..8314
                     /gene="nemR"
                     /locus_tag="OO326_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5966058.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8351..9448
                     /gene="nemA"
                     /locus_tag="OO326_001642"
     CDS             8351..9448
                     /gene="nemA"
                     /locus_tag="OO326_001642"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5966059.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9529..9936
                     /gene="gloA"
                     /locus_tag="OO326_001643"
     CDS             9529..9936
                     /gene="gloA"
                     /locus_tag="OO326_001643"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5966060.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10039..10686
                     /gene="rnt"
                     /locus_tag="OO326_001644"
     CDS             10039..10686
                     /gene="rnt"
                     /locus_tag="OO326_001644"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5966061.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10779..12893
                     /locus_tag="OO326_001645"
     CDS             10779..12893
                     /locus_tag="OO326_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5966062.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12903..14195
                     /locus_tag="OO326_001646"
     CDS             12903..14195
                     /locus_tag="OO326_001646"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966063.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15145..15237
                     /locus_tag="OO326_001647"
     CDS             15145..15237
                     /locus_tag="OO326_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5966064.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15281..16489)
                     /locus_tag="OO326_001648"
     CDS             complement(15281..16489)
                     /locus_tag="OO326_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5966065.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16497..16997
                     /gene="tnpA"
                     /locus_tag="OO326_001649"
     CDS             16497..16997
                     /gene="tnpA"
                     /locus_tag="OO326_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5966066.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(17036..17383)
                     /gene="grxD"
                     /locus_tag="OO326_001650"
     CDS             complement(17036..17383)
                     /gene="grxD"
                     /locus_tag="OO326_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5966067.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17718..18545
                     /gene="mepH"
                     /locus_tag="OO326_001651"
     CDS             17718..18545
                     /gene="mepH"
                     /locus_tag="OO326_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5966068.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARDSSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18673..19254
                     /gene="sodB"
                     /locus_tag="OO326_001652"
     CDS             18673..19254
                     /gene="sodB"
                     /locus_tag="OO326_001652"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5966069.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19400..20569)
                     /locus_tag="OO326_001653"
     CDS             complement(19400..20569)
                     /locus_tag="OO326_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966070.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20735..20824)
                     /gene="ynhF"
                     /locus_tag="OO326_001654"
     CDS             complement(20735..20824)
                     /gene="ynhF"
                     /locus_tag="OO326_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5966071.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21123..22148
                     /gene="purR"
                     /locus_tag="OO326_001655"
     CDS             21123..22148
                     /gene="purR"
                     /locus_tag="OO326_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5966072.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22145..23077)
                     /locus_tag="OO326_001656"
     CDS             complement(22145..23077)
                     /locus_tag="OO326_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5966073.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23190..24401
                     /locus_tag="OO326_001657"
     CDS             23190..24401
                     /locus_tag="OO326_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5966074.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24692..25840
                     /gene="cfa"
                     /locus_tag="OO326_001658"
     CDS             24692..25840
                     /gene="cfa"
                     /locus_tag="OO326_001658"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5966075.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25880..26521)
                     /gene="ribE"
                     /locus_tag="OO326_001659"
     CDS             complement(25880..26521)
                     /gene="ribE"
                     /locus_tag="OO326_001659"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5966076.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26736..28109
                     /gene="mdtK"
                     /locus_tag="OO326_001660"
     CDS             26736..28109
                     /gene="mdtK"
                     /locus_tag="OO326_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5966077.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28150..29406)
                     /locus_tag="OO326_001661"
     CDS             complement(28150..29406)
                     /locus_tag="OO326_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5966078.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29714..29790
                     /locus_tag="OO326_001662"
     tRNA            29714..29790
                     /locus_tag="OO326_001662"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29748..29750,aa:Val,seq:gac)
     gene            29795..29871
                     /locus_tag="OO326_001663"
     tRNA            29795..29871
                     /locus_tag="OO326_001663"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29829..29831,aa:Val,seq:gac)
     gene            29979..30284
                     /locus_tag="OO326_001664"
     CDS             29979..30284
                     /locus_tag="OO326_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5966079.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30410..31832
                     /locus_tag="OO326_001665"
                     /pseudo
     CDS             30410..31832
                     /locus_tag="OO326_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31844..32656)
                     /locus_tag="OO326_001666"
     CDS             complement(31844..32656)
                     /locus_tag="OO326_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966080.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32660..33445)
                     /locus_tag="OO326_001667"
     CDS             complement(32660..33445)
                     /locus_tag="OO326_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5966081.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33442..34110)
                     /locus_tag="OO326_001668"
     CDS             complement(33442..34110)
                     /locus_tag="OO326_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5966082.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34174..34812)
                     /locus_tag="OO326_001669"
     CDS             complement(34174..34812)
                     /locus_tag="OO326_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5966083.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34825..36926)
                     /locus_tag="OO326_001670"
                     /pseudo
     CDS             complement(34825..36926)
                     /locus_tag="OO326_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36947..37573)
                     /locus_tag="OO326_001671"
     CDS             complement(36947..37573)
                     /locus_tag="OO326_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5966084.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38029..>38110)
                     /locus_tag="OO326_001672"
     CDS             complement(38029..>38110)
                     /locus_tag="OO326_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2767 family protein"
                     /protein_id="HCR5966085.1"
                     /translation="ALANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9176 a         9950 c         9349 g         9635 t
ORIGIN      
        1 gggccagtct acaaccagtt gattaaaggc gctacaaccg gagagaagga ccggcaaaat
       61 gataattaac agttttttca tagtcatatc ccgaagactt tcctggtccg gagggcaaat
      121 accctcctta atcttccaag tgtaacggca gacacgataa gaaaaaattc agttaattct
      181 gatatcacga gttggcgggg aaaatagccc ccagtaccgt ctcctggctt gcgccagtga
      241 cggaaggcag atttcctggt aatcccgcca gcgtccgcca ggcaagccag gcgaaagcca
      301 atgcttccat gtcatcgcca ctaatgccaa cggcatcggt ggtggtgact tctgtgccta
      361 gcagtaatac cgccagacgc gccatgagta gcggattacg actacctcca ccacatacca
      421 tcaatcgttc gcagccaccg ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt
      481 ctgccagtgt ggcctgaaca tcacggggat caacacccgg aaaatggcgt aaatggcgct
      541 ccagccagcc gtagttaaag tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat
      601 acgggtcgct gagcatattt tgcagcagtg ggagaataac tttacccgcc cgtgcccact
      661 cggcatcttt atcgtaaggt ttacctgcct gacgccagat ccaggcatcc atcagcatgt
      721 taccaggacc ggtatcgtag ccgccaacca gctgcccagg aatgagcagt gacagattgg
      781 cgatgccgcc aatattgagc accattcgtc gctcggttgg gtgagccagc aaggcatgat
      841 ggaacgcagg taccagcggt gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat
      901 caccgacaac cgtaattccg gtgcgtgcca caatttgatt gttatcgcca atctgtaaag
      961 tgtgtggtgc cacgcccgtc ggttcatgcc agacggtttg accgtgacag ccgatcgcaa
     1021 ctatatctct cgcctgcagg ttttgttcct taagcaaggc attgaccgca tcagcaaaaa
     1081 gtcgcccgag ttgagtatca agctgtccaa actgcgaaag tgtaagctgc tggccctggc
     1141 aaatatccag tacagcctgt ttcagagaca ccgggatcgg ccagctcaag cttgccagct
     1201 gtgcgacccg gtgttcatca attgtcgcca acacaacatc aacaccatca aggctggtgc
     1261 ctgacataac gccaataaag cggcccgatt tcatagttca tcctttttca atctgacgtt
     1321 tgcgcaccac tcaaacataa acttttcgtg aataccatgc ggaatgaccg atttttaccg
     1381 ttggtagtaa aacattatct tcaaatcaat aatcatcatg aatgttttgt ttataattgg
     1441 ttgatcctac tttcattatg atttgctcat atttggtaga acatgtaacc atggattcac
     1501 atatgccata tactttgacc atgagggatg cttgcgtggc gtttcatggt gaacaggaga
     1561 tttttcaatg attaaacgcg tattggttgt ttcaatggta ggtctgtctc ttgtcggttg
     1621 tgttaataac gacaccctgt caggggatgt ttataccgct tctgaagcga aacaagtaca
     1681 gaatgtcagc tatggcacca tcgttaacgt acgtccggta cagattcagg gcggtgatga
     1741 ttccaacgtt atcggtgcaa ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt
     1801 tggtggcgga accgggcgtt ctctggctac tgcagcaggc gctgttgcag gtggcgtagc
     1861 tggtcagggc gtacagagtg caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa
     1921 agacgatggt aataccatca tggtggtaca gaaacaaggc aacactcgtt tctctccggg
     1981 ccaacgtgtc gtactggcca gcaatggcag tcaggtgacc gtttctccgc gctaaataag
     2041 tttgcgtgtg gtcaggttac tgaccacacg cccccttcat ttcacccttt ggcctgtaac
     2101 tcaatgatat tatgctcaag ttttgcgatg agcgtaatca gttgctccag ttcctctgcg
     2161 gagatgccat gtaatatttc cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc
     2221 agtggctctg ccttttccgt cagtttaata cgtttagccc gacgatcgct ggcacaagtt
     2281 tgacgcgaaa ttaacccttt ttcttccagt tggtccagag tacggaccag tgatggctgc
     2341 tcgatgccaa tcgcttttgc cagttgaatt tgcgactggt ctggaggtaa ctgatggata
     2401 ttgtgtaacg taacccaatg ggtttgtgtt aactccagcg gtttcaggcg atggtctatc
     2461 agagcacgcc atatgcgcac caaccgtgcc agatcagaac ctagtggcga ttccaatttc
     2521 atctccttat aattagcttg ctaagatatt atgcggcttt tagaatagtg tgcagcaatt
     2581 gtattgctaa aacaaatgta ttgctgcatt tggttaccgt cagacatatt tttcagaaat
     2641 tgcgcgtaaa tttttcgcac ttaaagaata tttattaatc taacgcaata tattcggtcg
     2701 taaaggaatc tactttgtga agtttatgct caatgcaaca ggattgccct tacaagatct
     2761 ggtgttcggt gcgtccgtct attttcctcc gtttttcaaa gcattcgcgt ttggattcgt
     2821 catctggctt gtcgtacacc gcctgcttcg tggctggatc tacgccggcg atatctggca
     2881 tcccttgtta atggatttat cgctgtttgc gatttgcgtt tgccttgctc tggcaatact
     2941 gattgcgtgg taactatgtc aattaaaaca attaagtatt tctcaacaat cattgtagcg
     3001 atagttgcgg ttcttgccgg atggtggctg tggaattatt acatgcaatc tccgtggaca
     3061 cgcgacggga aaatacgcgc agaacaggtt tctatcaccc cacaggtgtc gggacgtatt
     3121 gttgagctga atataaaaga caaccaactg gttaacgcgg gggaacttct gctcacgatc
     3181 gataaaacgc cctttcagat cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa
     3241 tctgacctcg caaaagccaa taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt
     3301 atttttgccg aagaactcga taccgctaac ctcaatgtta aagcgatgca ggccagtgtt
     3361 aatgccgcac aggcgacgct gaaacagaca caatggcaac tggcgcaaac ggaaattcgc
     3421 gccccggtga gtggatgggt gactaacctc actacccgca tcggcgatta cgctgacacc
     3481 gggaaaccgc tgtttgccct tgtcgacagc cactcgtttt acgtcattgg ttattttgaa
     3541 gaaaccaagt tgcgccatat ccgcgaaggt gcaccagcac aaattaccct ctatagtgat
     3601 aacaaaacgt tacagggtca cgtttccagt atcggtcggg cgatttatga tcagagcgtt
     3661 gaaagtgact caagccttat tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc
     3721 caactcgttc ccgtccgctt tgcgctggat aaagttccag gcgatgtcac gctggtgtcg
     3781 ggcaccacct gtagcattgc cgtaggtcaa taatgaacgc atcgtcatgg tccttgcgca
     3841 atttgccctg gttcagggcc acgctggcgc aatggcgtta tgcgttacgc aataccattg
     3901 ccatgtgtct ggcgctgacg gttgcctatt atttgaatct ggatgaaccc tattgggcga
     3961 tgacctcggc tgcagtggtt agctttccca ccgttggcgg tgtcatcagc aaaagcctcg
     4021 gacgcatggc tggcagttta ctgggagcca ttgcggcact gcttcttgcc ggacatacgc
     4081 tcaatgagcc gtggtttttt ctattgagca tgtcggcgtg gcttggcttt tgtacctagg
     4141 cctgtgcgca cttcacgaat aacgtcgcgt atgcatttca actggcgggc tacacggctg
     4201 ccatcatcgc ctttccgatg gttaatatta ctgaggccag ccagctgtgg gatatcgctc
     4261 aggcgcgcgt ttgcgaggtg atagtcggta ttttgtgcgg cggcatgatg atgatgatcc
     4321 tgcctagcag ttccgatgct actgcccttt taactgcatt gaaaaacatg cacgcccgat
     4381 tactggaaca tgccagttta ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac
     4441 atgagggggt gattgggcag atactgacca tgaatttgct gcgtatccag gctttctgga
     4501 gccactatcg ttttcgccag caaaacgcgc gccttaatgc gctactccac cagcaattac
     4561 gtatgaccag tgtcatctcc agcctgcgac gtatgttgct caactggccc tcaccgccag
     4621 gtgccacgcg agaaattctc gaacaattgc tgacggcgct cgccagttcg caaacagatg
     4681 cttacaccgt cgcacgtatt atcgccccgc tacgcccgac caacgtcgcc gactaccggc
     4741 acgtcgcctt ctggcagcga ctacgttatt tttgccgcct ttatctgcaa agtagtcagg
     4801 aattacatcg tctgcaaagc ggtgtagatg atcatgccag actcgcacgg acatccgctc
     4861 tggctcgtca taccgacaac gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt
     4921 tgatgatgat tggcgcatgg agtattgcat cgcaatggga tgccggtgcc aatgcattaa
     4981 cgctggcagc aattagctgc gtactctact ccgccgtcgc agcaccgttt aagtcgttgt
     5041 cactgctgat gcgcacgctg gtgttacttt cgctattcag ctttgtggtc aaatttggcc
     5101 tgatggtcca gattagcgat ctgtggcaat ttttactgtt tctctttcca ctgctggcga
     5161 caatgcagct tcttaaattg cagatgccca aatttgccgc actgtggggg caactgattg
     5221 tttttatggg ttcgtttatc gctgtcacta atcccccggt gtatgatttt gctgattttc
     5281 ttaactataa tctggcaaaa atcgttggcg ttgcgctggc ttggctagcg ttcgccattc
     5341 tgcgtccagg atcggatgct cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt
     5401 ttgtcgatca gctaagccgc catccaacac tgagtgaaag cgaatttgaa tcgctcactt
     5461 atcatcacgt cagtcagttg agtaacagcc aggatgcgct ggctcgccgt tggttattac
     5521 gctggggtgt agtgctgctg aactgttctc atgttgtctg gcaattgcgc gactgggaat
     5581 cgcgttccga tccgttatcg cgagtacggg ataactgtat ttcactgttg cggggagtga
     5641 tgagtgagcg tggcgttcag caaaaatcac tggcggccac acttgaagaa ttacagcgga
     5701 tttgcgacag ccttgcccgt catcatcaac ctgccgcccg tgagctggcg gcaattgtct
     5761 ggcggctgta ctgctcgctt tcgcaacttg agcaagcacc accgcaaggt acgctggcct
     5821 cgtaattact taattacacc acaggcatag cgttcaccgc caccgcccag cggtttaggt
     5881 tgatcggaca tattatcgcc gccaacgtgg accatcagcg ccttgtcttt gatttcatcc
     5941 agtgatttca gacgaggcgc gatgacggca tcggtagctt tgccgtcatt attgacgacc
     6001 agtgcaggca gatcgcctaa atgcccggta ccttctggcc cttcatgttt accggtattt
     6061 tgtggatcaa gatgcccgcc tgcggattcc gcggcgctgg ctttgccatc tttggtggct
     6121 ggctggcagc ttcctttggc atgaatatgg aagccatgtt caccgggggg taatgctttc
     6181 agatcgggtg aaaactccag acctttatcg gtttcagtaa tggtgacgct accaattgac
     6241 tgcccaaccc cttgcgacgt gacgaggttc atctcgactt tttcactggc agcttgtgcg
     6301 ccggtcgcaa caaccagcgc cagaatggcc agactaaaac gtttcatagg acctccgttc
     6361 attgtgcatt cctgaataag tgtaaaccag tgacacattt ttgaacggcg gctattccta
     6421 aaagtgctta cggtacgtcg taccccagtg cagctttacg gatacgaaac cattgttgac
     6481 gggtcatttt cagtgtttct gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc
     6541 cgataattgg cagcggctgc gacggtaaac gtaataccca ggcgtaaacc acctgttcaa
     6601 tcgagcccgc gtttaactcc tccgccacca cagccagttc atcacgcagc ggctggaaat
     6661 aatcatcatt aaacagacga ccaccaccaa ggcaggacca cgccatcgga cgaacacgca
     6721 gttgttgtag ttggtcgagc gtgccatcca gcagtaacgg ctgatgcacc ggggatattt
     6781 ccacctgatt agtggcaagg gtaaacggca gacgtgattg caacagggca aattgcgcag
     6841 gcgtaaagtt cgatacgcca aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg
     6901 cgtccgccac ttcatcggca tccattaacg ggtctggtcg gtggattaac agcagatcca
     6961 gatgatcggt cgcgagatta attagcgact gttcggcgct cttaatgatg tgattgcggt
     7021 cagtgatgta atgaccaatg acgttttctt cacgcgcggt cgtcgcgata ccgcatttac
     7081 tgacgatttc catccgttca cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg
     7141 cttcgcactg atagccacca taaatatcag catggtccac ggtggtcacg ccgagatcca
     7201 gatgctcttc aataaaactg accagctggc gggcggacat attccagtcc atcaatcgcc
     7261 agtagcccat cacaaaacgg gaaaactccg ggccttccgg cgcaatagta atacgctgaa
     7321 ccataatcgc ttcctcttat cagatataag aggagtatac gcaagattag gttcaaaaga
     7381 gtgatggttg ctccggttcg tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta
     7441 atcgctgccg acaaaggcgc agcacctctt gtttttcgcc atcgctcatt ttattccagt
     7501 taaaacgctc atcccgacta cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc
     7561 aaattccccg gcacgggctc tggacgggaa agaactctaa ttgctccgcc acctcgccct
     7621 cctcagataa gattattacc attattgaag ctgttaatgt ccaaagtagc aactttgctt
     7681 gcactagacc aactggtcta ctacactcca acgcatgaac aaacacaccg aacatgatac
     7741 tcgcgaacat ctcttggcga cgggcgagca actttgcctg caacgtggat tcaccgggat
     7801 ggggctaagc gaattactaa aaaccgctga agtgccgaaa gggtccttct atcactactt
     7861 tcgctctaaa gaagcgtttg gcgttgccat gcttgagcgt cattacgccg catatcacca
     7921 gcgactggct gagttgctgc aatccggcga aggtaactac cgcgaccgca tactggctta
     7981 ttaccagcaa acactgaacc agttttgcca acatggaacc atcagtggtt gcctgacagt
     8041 aaaactctct gccgaagtgt gcgatctgtc agaagatatg cgcagcgcga tggataaagg
     8101 tgctcgcggc gtgatcgccc tgctctcgca ggctctggaa aatggccgtg agaaccattg
     8161 tttaaccttt tgtggcgaac cgctacaaca ggcacaagtg ctttacgcac tatggcttgg
     8221 cgcgaatctg caggccaaaa tttcgcgcag tttcgagcca ctggaaaacg cgctggccca
     8281 tgtaaaaaac attattgcga cgcctgccgt ttagcaggca ttttttatca ccagacgacc
     8341 gggagccttt atgtcatctg aaaaactgta ttccccactg aaagtgggcg cgatcacggc
     8401 ggcaaaccgt atttttatgg caccgctgac gcgtctgcgc agtattgaac cgggtgacat
     8461 tcctaccccg ttgatggcgg aatactatcg ccaacgtgcc agtgccggtt tgattattag
     8521 tgaagccacg caaatttctg cccaggcaaa aggatatgca ggtgcgcctg gcatccatag
     8581 tccggagcaa attgccgcat ggaaaaaaat caccgctggc gttcatgctg aaaatggtca
     8641 tatggccttg cagctgtggc acaccggacg catttctcac gccagcctgc aacctggcgg
     8701 tcaggcaccg gtagcgcctt cagcacttag cgcgggaaca cgtacttctc tgcgcgatga
     8761 aaatggtcag gcgatccgcg ttgaaacatc catgccgcgt gcgcttgaac tggaagagat
     8821 tccgggtatc gtcaatgatt tccgtcaggc cattgctaac gcgcgtgaag ccgcttttga
     8881 tctggtagag ctccactctg ctcacggtta tttgctgcat cagttccttt ccccttcttc
     8941 aaaccatcgt accgatcagt acggcggcag cgtggaaaat cgcgcacgtt tggtactgga
     9001 agtggtcgat gccgggattg aagaatgggg tgccgatcgc attggcattc gcgtttcgcc
     9061 aatcggtact ttccagaaca cagataacgg cccgaatgaa gaagccgatg cactgtatct
     9121 gattgaacaa ctgggtaaac gcggcattgc ttatctgcat atgtcagaac cagattgggc
     9181 ggggggtgaa ccgtatactg atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc
     9241 gattatcggc gcaggtgcat acacagtaga aaaagctgaa acgctgatcg gcaaagggtt
     9301 aattgatgcg gtggcatttg gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt
     9361 gcagctcaaa gctgagctta acccacagcg tgccgaaagt ttctacggtg gcggcgcgga
     9421 aggctatacc gattacccga cgttgtaatc caacattgcg agcggcgtaa agccgccgct
     9481 atactaaaac aacattttga atctgttagc cattttgagg ataaaaagat gcgtcttctt
     9541 cataccatgc tgcgcgttgg cgatttgcaa cgctccatcg atttttatac caaagtgctg
     9601 ggcatgaaac tgctgcgtac cagcgaaaac ccggaataca aatactcact ggcgtttgtt
     9661 ggctacggcc cggaaaccga agaagcggtg attgaactga cctacaactg gggcgtggat
     9721 aaatacgaac tcggcactgc ttatggtcac atcgcgctta gcgtagataa cgccgctgaa
     9781 gcgtgcgaaa aaatccgtca aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa
     9841 ggcggtacta cggttatcgc gtttgtggaa gatccggacg gttacaaaat tgagttaatc
     9901 gaagagaaag acgccggtcg cggtctaggc aactaatctc ctgccgggcg tgaactcatc
     9961 gcgcccgcat ctttactgca tcgacaagta atatttgtca taatgcgcgc tgcaatttat
    10021 ccgtattaag agaatcagat gtccgataac gctcaactta ccggtctgtg cgaccgtttt
    10081 cgtggttttt atcctgttgt gatcgatgtt gaaacagccg gatttaacgc caaaaccgat
    10141 gcgctgcttg agattgccgc catcaccctg aaaatggatg aacaaggctg gctgatgccg
    10201 gacaccacat tacatttcca cgtcgaacca tttgtcggcg caaatttgca accagaagcc
    10261 ctcgccttca acggcattga cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag
    10321 gcgctgcacg aaatttttaa agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc
    10381 gccattatgg tggcgcacaa cgccaatttt gatcacagct ttatgatggc cgccgcagaa
    10441 cgcgcctcgc tgaaacgtaa cccgttccac cctttcgcca cttttgacac tgctgcactg
    10501 gccgggctgg cactcggaca aaccgtattg tcaaaggctt gccagaccgc tggcatggac
    10561 ttcgacagca cccaggcgca ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt
    10621 tgtgaaatcg tcaaccgctg gaaacgtctg ggaggctggc cgctacctgc cgccgaagag
    10681 gtgtaatcga gtcgatgcct gatgacatgc aatgattcag gcatctatag tgaggctatt
    10741 ccacgcatca agcatgatat tcacggggaa tagcgttaat ggcagataat ccagaccctt
    10801 catcgctcct gccggacgtg ttttcaccgg cgacccgcga ctggtttctt cgcgccttta
    10861 aacagccgac cgctgtccag tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc
    10921 tggtgattgc accgactggc tccgggaaaa cgctggcagc atttctctac gccctcgatc
    10981 ggctcttccg cgaaggcggc gaagataccc gcgaggcgca taagcgtaaa acctcacgca
    11041 tcctctatat ttcaccgata aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc
    11101 cgttgaaggg tattgccgat gaacggcggc ggcgcggcga aacggtaatt attgatgaag
    11161 tccacgcggt agcgggcagt aaacgtggtg cgcatctggc gttaagtctg gagcggctcg
    11221 atgcgctgct ccacacctca gcacagcgaa ttggcctttc tgccactgtg cgctcagcca
    11281 gcgatgtggc agcatttctt ggtggcgatc gcccggttac ggtagtcaac ccgcccacaa
    11341 tgcgccatcc gcagatacga attgtcgtac cggtcgccaa tatggatgat gtctcatcgg
    11401 tcgccagcgg caccggcgaa gacagccatg ccggccggga aggctccatc tggccatata
    11461 ttgaaacggg tatccttgat gaagtgttgc gccatcgctc gaccattgtc tttactaatt
    11521 cacgcgggct ggcggaaaaa ctgacggcac gattaaatga gctttacgcc gcacgcttac
    11581 agcgttcccc gtctatcgcc gttgatgcgg cccatttcga gtcgacctcc ggcgcaacct
    11641 ctaaccgtgt acaaagtagc gacgttttta ttgcccgctc acaccacggc tccgtctcta
    11701 aagaacaacg agcaatcacc gaacaggcgc tgaaatcggg tgaattacgc tgcgtggtcg
    11761 caacctccag tcttgaactg gggattgata tgggcgcggt ggatctggtg attcaggtgg
    11821 caacgccgct ttctgttgcc agtgggttac aacgcattgg tcgcgccgga catcaggttg
    11881 gcggtgtatc taaagggctg tttttccccc gtacccggcg tgatttagtc gattccgcag
    11941 tcattgtaga gtgtatgttc gcaggcaggc tggaaaacct gacaccaccg cataatcctc
    12001 tcgacgtcct tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta caggtagacg
    12061 aatggtactc ccgcgtacgc cgtaccgcac cgtggaaaga tctgccaaga cgtgtttttg
    12121 acgccacgct ggatatgctt tccgggcgct atccctctgg cgatttttct gcttttcgcc
    12181 ccaaactggt ctggaacagg gagaccggga tattgaccgc ccgacctggc gctcaattgt
    12241 tggcggttac cagcggcggc accattccgg atcgtggcat gtatagcgtg ttattacccg
    12301 aaggtgaaga aaaggccggt tcgcggcggg tgggtgaact ggatgaggag atggtatatg
    12361 agtcgcgggt gaacgacatt atcactctcg gcgctacctc atggcggatc cagcaaatca
    12421 cccgcgatca ggtgattgtg actcctgctc cgggtcgttc tgcccagctc cccttctggc
    12481 gtggtgaagg taacggacgt ccggctgaat taggcgagat gatcggcgat tttcttcatt
    12541 tactggcgga tggcgcgttc ttttccggga ctattccccc gtggctggca gaagaaaata
    12601 cgaacgccaa tattcagggg ttgattgacg agcaacgcaa cgcgacgggc atcgttccgg
    12661 ggagtcgcca tctggtcctc gaacggtgcc gtgatgaaat tggcgactgg cgtattattt
    12721 tgcactctcc ctatggaaga cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa
    12781 tacatgccct atggggcgct gacgcgtctg tggtcgccag tgatgacagc attgttgcgc
    12841 gcattcctga cactgatggt aaattgcccg acgccgcgat ttttttgttt taaccagaaa
    12901 agttgctgca aattgtccgc gaggcggtag gcagctcggc acttttcgcc gcccgttttc
    12961 gcgaatgcgc cgcgcgggca ttattaatgc cgggccgcac gccgggccat cgcaccccgc
    13021 tttggcaaca acggctgcgc gccagtcagt tgctggaaat cgctcaggga tatccggatt
    13081 ttccggtcat tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat cttcccgcac
    13141 tggaacgttt gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat gtaacgacca
    13201 ctacgccctc gcctttcgcc acaagtttat tgttcggcta tgtcgcggaa tttatgtacc
    13261 agagcgacgc cccgctggca gagcgccggg catccgtact gtcgctggac agcgagttac
    13321 tgcgcaatct actcggacag gtcgatccgg gggaattact cgacccgcag gtcattcgcc
    13381 aggtggaaga agagttgcaa cgactggctc ctggtagaag agcgaaaggt gaagaaggat
    13441 tgttcgacct gctgcgcgaa ctggggccaa tgaccgttga agacctggcg caacggcata
    13501 caggcagcag tgaagaggtt gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct
    13561 tcccagcgat gattagcgga caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc
    13621 gtgatgccct cggcgtacgg ctaccagagt cattgccaga gatttattta catagagtca
    13681 gttacccgct tcgcgacctc tttctgcgct atctccgggc tcatgctctg gtcacggctg
    13741 aacaactggc tcatgagttt agtctcggta ttgccattgt cgaagagcag cttcagcaac
    13801 tgcgtgaaca gggtctggtg atgaatctgc aacaagacat ctgggtgagc gatgaagtat
    13861 ttcgtcgtct gcgtttgcgc tcgctgcaag ccgccagaga agcgacgcgt cccgttgcag
    13921 ccacgaccta tgcgcgattg ctgctggaac gtcagggcgt attacccgcc accgatggta
    13981 gcccggcgct ctttgcctca acatctccag gcgtttatga gggcgtagat ggagtgatgc
    14041 gggtgatcga acagcttgcc ggagtcggtt tacccgcctc actctgggaa agccagatcc
    14101 tgcctgcccg cgtacgcgac tattcgccag aaatgctcga tgaattactg gcaaccggag
    14161 cggttatctg gtcgggacaa aaaaaagctg ggtgaagatg acggcctggt ggcactgcat
    14221 ctacagaaat atgccgcaga atcgttcact cccgccgaag cggatcaggc gaatcgttcg
    14281 gcgctgcagc aagcgataat cgctgttctg gctgacggag gagcctggtt tgcacaacaa
    14341 atcagccaac ggatacgcga taaaatcgtc gaatcggttg attcctcggc actgcaagag
    14401 gcgttatggg cgctggtctg gcaaggcgtc attaccagcg atatttgggc accgttacgc
    14461 gccctcaccc gcagcagttc caacgcacgc acctcaactc gccgcagtca ccgggctcgt
    14521 cgtgggcgtc ctgtctatgc acaacccgtc tcgccgcggg tatcttacaa cacaccaaat
    14581 ctggctggac gctggtcgtt attgcaggtg gagccactaa acgataccga aaggatgctg
    14641 gcgctggcgg aaaatatgct cgaccgctac ggcatcatca gtcgtcaggc ggtgatagcc
    14701 gaaaatatcc ctggcgggtt tccatcgatg caaacacttt gtcgaagtat ggaagactcc
    14761 gggcgaatta tgcgaggtcg ttttgtagaa ggtctgggtg gcgcgcaatt cgctgaacgt
    14821 ctgactattg accgattgcg cgatctggcg acacaagcca cgcaaacacg ccactataca
    14881 ccagtggcgc tctccgccaa cgatccggct aatgtgtggg gaaatcttct gccctgggct
    14941 gcacatccgg caacgctggt accaacgcgt cgggcgggtg cgctggtggt cgtttctggc
    15001 ggcaaattgt tactctatct ggcgcaaggc ggcaaaaaaa tgctggtctg gcaggaaaag
    15061 gaggaattac tcgccccaga gattttccag gcgctgacca ccgcactgcg tcgcgaacca
    15121 aggctgcgct ttacgctaac agaagtgaat gatcttccgg tccggcaaac gccgatgttt
    15181 acgctgctgc gtgaggcggg attttcaagt tcgccacaag ggctggattg gggatagttg
    15241 acatcctcca cgccctgaaa gacgtggttc cctgctacgt tcaagctgtc gcctgaatca
    15301 tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa
    15361 cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc gctgtatatc
    15421 cacatacctg gcatctgaat ttactttgtg acaggcggtt ttctttcgct gtatgaccac
    15481 agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccactcc
    15541 agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc agtatcgaac
    15601 ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta cccgctgctg
    15661 actttgacat gttgctgacc ttcaaatcct caatgactat cattgcgtgg tttttgctga
    15721 cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag gaatgcagtc
    15781 gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttc cggcttaact
    15841 gtcgctgaag tctcgccagc gtcttctggt ttttttgaaa actgtttaca ggctcaaaga
    15901 ctgtgccatc tgacagcgtg gcgagtttag ccacgccagc atccagcccg atcattgatg
    15961 ctgaagggtg aaccggagtt gatacttcac tttctgtctg aatactgatg taccacgtac
    16021 cgcaggactg gctgacagtg acatttttca caactcccgt gacctgacgg ctattccggt
    16081 agcgcatcca gccaagtttc ggcagaaaaa tacggctgtt ttcctgatcg agcttaacgt
    16141 cctgcgggta gcggaatgca gcattctgtc cccgcttttt gaatcgggga aaagcagccc
    16201 gcttctggaa gaagtttttg taagcccgct caaggtcttt cagtgactgt tgcaatggct
    16261 gtgagggcga atctttaagc cattgcgttt cagtggcgtt tttccactca accagccagg
    16321 aagccatttt cccgtaaggg atgtatttat taccggcctc atgattctca ttctgaagcg
    16381 ccagagcacg attgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt tcacgctcct
    16441 gttgaccacc gggtcttaac tggaatttaa atgcctgtaa tcgtttcaca ggtcaaatga
    16501 tactttggtc tgtgaaaaaa gaaaccgata tccggcgtgg aaggcattgt gttttcctga
    16561 tgcatgttca cctggtcttt gtcaccaaat accgacgcca gatttttgac catgacacga
    16621 cagaaaaact acgcacttac ttttcaaatg tatgtgctga ttttgcagct gaactggttg
    16681 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
    16741 tatccagtct ggtaaacagc ctcaaaggca tatcgaatag gttactgcga cgagatcgac
    16801 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
    16861 gtagctgcga aggtgcgcca atatccgtca tccgccagta cattgaacaa cagcaaacac
    16921 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggacgaggt tttacggcgc
    16981 accggataaa ggactgacgg atgcccgttc gcatccgtca gtattgcagg acggattatt
    17041 ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc tttgatcagc tgctgcagtt
    17101 cgccacgctg atacatttcg atcacgatat cacaaccgcc gaccagctcg ccgtcaaccc
    17161 acagttgcgg gaaggtcggc cagttagcat atttcggcag ttccgcacga atgtccggat
    17221 tctgcagaat atcaacatag gcaaaacgtt cgccacatgc ggcaagcgcc tggactgcct
    17281 gggcagagaa accgcagctc ggcagtttcg gtgaaccttt catgtacagc aggatcgggt
    17341 tttcagcaat ctggcgttgg attttttcga tagtggtgct cattgtcttg cttcctcaaa
    17401 cttctttacg gcagtaatct gacattgtag cgggtcagtg cggcatcgga aaataacatt
    17461 ttcatcacgc ttttgctatt ttatcccttt gctctatttg ttgcatttca aatattcgtt
    17521 tcttctctga tgcgcggcaa actggcgtat ttataaccat ttttagcttt cactgctgca
    17581 tttttttgcg ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt
    17641 cttctatgca ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct
    17701 taacagaggg acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac
    17761 tttttaccac cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggatagtt
    17821 ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa
    17881 agacccagaa agccagccag aaaacagcga aaaaagccgc cagtaaaagt atgaccaaaa
    17941 gcaaaaccgc ttcttctgtt aaaaaatctt ccattaccgc ttctaaaaac gccaaaactc
    18001 gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag tgtaccaaac
    18061 gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga acccttgccg
    18121 acgcgcacaa agcgaaggtg caaaaagcca cgaaagtggc aatgaataaa ctgatgcagc
    18181 aaattggtaa gccatatcgt tggggcggca gctcaccgcg taccggtttt gattgcagcg
    18241 gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt acggcgaatg
    18301 aaatgtatca cctgcgtgat gcaggaccta tcgaacgtag tgagctgaaa aacggcgacc
    18361 tggtcttttt ccgtactcag ggacgcggta cagccgatca tgtcggcgtg tatgtcggca
    18421 acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact tctctcagtg
    18481 aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca aaaacacttc
    18541 gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt ctcacctttt
    18601 aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa ttaataataa
    18661 aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga tgctctggca
    18721 ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca gacttatgtc
    18781 actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact ggaagagatt
    18841 attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa ccatactttc
    18901 tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt cgctgaagct
    18961 atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga tgcagcgatc
    19021 aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa actggctatc
    19081 gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct gctgaccgtt
    19141 gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg ctatctggag
    19201 cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc ataataactg
    19261 atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg atgaggcgtt
    19321 aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca gaaggttatc
    19381 cttctgcgtt tttgtttaat tagctgttag caacgcaaac tgtttcaggt tgttttctgg
    19441 ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccgtc accggcacaa
    19501 agctgtatcc cagcccagcg gaaattaccg caccaccagc agctgctccc agcgcatttc
    19561 caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca ctggcgacat
    19621 gcatcacgcg catctgtaac ggaggtacaa ccgcaaaggt tgctgcgccc cacaccacca
    19681 tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg gattgccagc ataatcacca
    19741 tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc agtttgccgc
    19801 cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc gtgacgaaca
    19861 ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag agggtaaaca
    19921 ttgcaccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga cgcattaata
    19981 ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt aatgagaaga
    20041 acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac atccgccagc
    20101 cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc agggttaacc
    20161 ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg cttgcggcca
    20221 cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaata cgtgaaagca
    20281 tcagggtcat ataatccggc gcgatggcgg aaagaacgtt gcctagcgtg aaaattgcca
    20341 tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc atcagcggcg
    20401 cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg ggaatcgaga
    20461 catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc gttgtcccga
    20521 taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg ccttatctcc
    20581 acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca gcaatcacaa
    20641 aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa acaggaagga
    20701 gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca gtcaaaccca
    20761 cggctacgat caaaccgagg acgataaccg ttgttaccag tgaaaattta aggtcggtgc
    20821 tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca tttttacaca
    20881 ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac ttcccctttt
    20941 cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat tgaccccttc
    21001 ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg taaggtgtgt
    21061 aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg gagtgaaatg
    21121 gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca actgtgtcac
    21181 acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg tgggcagcga
    21241 ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt aaccacacca
    21301 agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag atcattgaag
    21361 cagttgaaaa aaattgcttc cagaaaggtt acaccctgat tctgggtaat gcgtggaaca
    21421 atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc gatggtctgc
    21481 tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag tatcgccata
    21541 tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat gcggtcattg
    21601 ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc ggtcaccgcg
    21661 aaatcggcgt catccccggc ccgctggaac gtaacaccgg cgcaggccgc cttgccggtt
    21721 ttatgaaggc gatggaagaa gcgatgatca aggtgccgga aagctggatt gtgcagggtg
    21781 actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag ccgcatcgcc
    21841 ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt gctgctgatg
    21901 aaatgggcct gcgcgtcccg caggatgttt cgctgatcgg ttatgataac gtgcgcaacg
    21961 cgcgctattt tacgccggcg ctgaccacga tccatcagcc aaaagattcg ctgggtgaaa
    22021 cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg cagtccattg
    22081 aagtgcatcc gcgtttgatt gaacgccgct ccgtggctga cggcccgttc cgcgactatc
    22141 gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc ttattcagcg
    22201 tctcactatc gctgagatac tcaagcagcc aggttaacgc aggcgacata tcattttgct
    22261 gccatgtcag acaacatgcc gaatccggga aggggttttc cagttcaagt gctacccact
    22321 tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg cataatcctg
    22381 ccgagataca ggttgccgat gattcccagt caggtacgac aagtcttttt tggttatcca
    22441 gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc aacgacggcc
    22501 agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg tggtgactgg
    22561 caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga ccgcctaccg
    22621 gaatcgcgcg tgttgcgcca attgccagtt ccacgcgccc gtcggaaagc gcatcccaga
    22681 caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg cgataaaaat
    22741 caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg atagctaact
    22801 gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc atttttttga
    22861 caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc acatcacggt
    22921 gccgtcgttc aaagagcggt accgccagcc actcttccag ctgacgcacg gtatagctga
    22981 ccgcagaagg aacacgatgc agctcctgtg ccgcagcgct aaaactacca ttacgcgcta
    23041 ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa aatttttgaa
    23101 accagtcatc aaatattacc gtttcacaac actaatttca ctccctacac tttgcggcgg
    23161 tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt ttagtctggc
    23221 tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct gctttcgccg
    23281 ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt agtctgttcc
    23341 ttgccggttt tgccgcagcc cagcttctgt gggggccact ctccgaccgt tatggtcgta
    23401 aaccggtgtt attaatcggc ctgacaattt ttgcgttagg tagtctgggg atgctgtggg
    23461 tagaaaacgc cgccacgctg ctggtattgc gttttgtaca ggctgtgggg gtctgcgccg
    23521 cggcggttat ctggcaagca ttagtgacag attattatcc ttcacagaaa gtcaaccgta
    23581 tttttgcgac catcatgccg ctggtgggtc tgtctccggc actggctccc ctgttaggaa
    23641 gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt gccattaccg
    23701 tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt aacaatagtc
    23761 aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc aacgtgctga
    23821 tatacgcagc ctgttcagcc agtttttttg catggctgac cggttcaccg ttcatcctta
    23881 gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa actatcgcgt
    23941 ttctgattgg tggttatggc tgtcgcgccg cactgcagaa atggcaaggc aagcagttat
    24001 taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct gcgggcttca
    24061 ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg attgccaatg
    24121 gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacat gcaaccggtc
    24181 gcgccgcagc gttgcagaac actctgcaac tgggtctgtg cttcctcgca agtctggtag
    24241 tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg atgttatcaa
    24301 cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagtt gactgcccga
    24361 atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg atatacttat
    24421 acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat actaatttcg
    24481 agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt cccgactcac
    24541 tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc ggattataga
    24601 ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg ctgttttaag
    24661 gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag aagaagtcag
    24721 tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg ccggtatagc
    24781 cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt ttaaacgcgt
    24841 tctgcaggaa ggctctttgg ggttaggcga aagttatatg gatggctggt gggaatgtga
    24901 ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc aactccccca
    24961 tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc agagtaaaaa
    25021 acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt tcagccgcat
    25081 gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata atctggaatc
    25141 tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac cagggatgcg
    25201 cgtactggat attggctgcg gctggggcgg actagcgcac tacatggcat ctaactatga
    25261 cgtaagcgtg gtcggcgtca ccatttctgc cgaacagcaa aaaatggctc aggaacgctg
    25321 taaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg accagtttga
    25381 tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg atacctattt
    25441 tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc ctgctccata ctatcggttc
    25501 gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc cgaacggttg
    25561 cctgccctct gtacgccaga ttgctcaatc cagcgaaccc cactttgtga tggaagactg
    25621 gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac gattcctcgc
    25681 cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt ttacctatta
    25741 tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc aggtggtgtt
    25801 ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct atcgccccct
    25861 cttcgggggc gagttcagat caggcttctg tacctggttg attcatggca ttttctcgtg
    25921 ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatcg atttcaatgt
    25981 tgacgcgtgc gccaagtttt ttcttcccaa gagtcgtgcg ttccagtgtt tccggaatta
    26041 aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata ccgtcgatgc
    26101 caataaatcc tttgtacagg atatatttca tcaactgact atcctggact ttaaaccaga
    26161 tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc ataatatgac
    26221 ctgacattaa gtgtccgcca atttcatcac tgaatttcgc cgcacgctca acgtttaccc
    26281 aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc atcaggtcaa
    26341 aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta tgcgccacgg
    26401 aagcaccggt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca tgcgtacgaa
    26461 aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc cccgtaaaca
    26521 tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca ggtggaaaac
    26581 gcccttacca gtgaaggggt aagaatggct attttttcac tggagaatta ataaatcctc
    26641 gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag gcggcttttt
    26701 agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc agtgaagcgc
    26761 gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa actgcgatgg
    26821 gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg gcggtcgcta
    26881 tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg ctggcattaa
    26941 cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg catcaggtgc
    27001 gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg ctgtggaatg
    27061 caggttacat tatccgctac atggaaaaca tcgatccggc tctggcggac aaagccgtgg
    27121 gttatctgcg tgcgttgttg tggggcgcgc cgggatatct gttcttccag gttgcccgta
    27181 accaatgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt atcggcctgc
    27241 tggtgaacat cccggtgaac tatatcttta tttatggtca tttcggtatg cctgagctcg
    27301 gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggtcatgttc cttgccatgg
    27361 tttcttacat taaacgcgcc cgctccatgc gcgatattcg taacgaaaaa ggcaccgcta
    27421 agcccgagcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt gcgctggcac
    27481 tgttctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg ctcggtattg
    27541 ttgatgtcgc aggacaccag attgccctga actttagttc actgatgttt gtgcttccaa
    27601 tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag ggctcaacgc
    27661 tggatgcgca aaccgctgcg cggaccgggc ttatggtggg tgtctgtatg gcaaccctga
    27721 cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac gacaatcccg
    27781 aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag atttctgact
    27841 caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt tccattttct
    27901 atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt ctggcactga
    27961 ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt attatcggcc
    28021 tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt atgccgtcag
    28081 ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc acgggttgcg
    28141 cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg gctgtaattt
    28201 cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta tatccccgtt
    28261 aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg tttgcaggca
    28321 aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg ccgcaccagt
    28381 gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag tgacgctgtc
    28441 accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag taatcgttga
    28501 ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta atgcaacact
    28561 cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt ccgcgctggt
    28621 gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg taccggcatg
    28681 cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg aactgtagag
    28741 ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag tgtcagagtc
    28801 ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg ctttcccggc
    28861 cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac ttggatacac
    28921 caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa ccaacaggcc
    28981 gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac gcaggttacc
    29041 gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt caccttcggg
    29101 atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct cggactgaac
    29161 aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac cgtactgtgt
    29221 gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga ccacggtatt
    29281 ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg tcgcgccaag
    29341 gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt tcgcatcaga
    29401 tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa gtgtaaaggc
    29461 tacggaggat ttatccacga cagatttgag atggtggcaa acaactctgt ttaaactctg
    29521 atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa atcgcccttg
    29581 caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag
    29641 aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatattcg tccccgttgt
    29701 cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg
    29761 tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc
    29821 accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt
    29881 aatctggtct tttccttttt ccctctgttt cttctctgta tccaatacgt taaaagattt
    29941 acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg ttacaacttc
    30001 attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa ccacttgctg
    30061 agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc
    30121 acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc tatctggaaa
    30181 aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg
    30241 tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat ttaatcaagg
    30301 gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa tctcaaccaa
    30361 acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat
    30421 tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc tacagcaaca
    30481 aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg ggatgccata
    30541 cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg aaataccgcc
    30601 gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc tccagcgtta
    30661 tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc tgctgggcga
    30721 atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa aatttgcagt
    30781 ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg gcgtcatgct
    30841 cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg ccacgggtca
    30901 cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt ggtcaggcct
    30961 gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct tgagcggact
    31021 ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg ataaacaaca
    31081 cgtcgttttt caccgcgata acgcaagtga aaagctaaat attacgttga tgtcgcgcac
    31141 gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct tacacatcgt
    31201 caccgatcag gcattaaatg ctgagattca aaaaggtgaa gagggccttt tggatcgggt
    31261 atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc aacgcatagc
    31321 cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc gcaaacaacg
    31381 cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa
    31441 acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac aggaaattgt
    31501 tcctcatctg aatgaacacg accataaacg gttcagtaaa ggccttgcca gggttttcat
    31561 cgataattat gcggcaatcc cttcagagtc tattcgtcga ctactggcct tacgtgaagc
    31621 ggggatctga aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc
    31681 gtggacgcgt agcgttcggc gcgttgccct ggttgatgca cgaccagcct ttcgttcagg
    31741 gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat
    31801 cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact
    31861 gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg
    31921 cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctagcc
    31981 cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa
    32041 tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt
    32101 gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc
    32161 gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac
    32221 cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac
    32281 ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga
    32341 ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac
    32401 tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat
    32461 gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc
    32521 agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta ccgccgcgtg
    32581 ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg
    32641 ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt
    32701 cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta
    32761 taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc
    32821 ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat
    32881 acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg
    32941 ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg
    33001 ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat
    33061 tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg
    33121 caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccacttg
    33181 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt
    33241 aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca
    33301 aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
    33361 acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact
    33421 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
    33481 gcgatacaga tgcggttttc cggcaccagg tagctgatat tgatagtatt tattatcctg
    33541 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca
    33601 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
    33661 tttatctgcc actttagtga cagggttaag gtagcgaacc aggtacggac aagcgccaat
    33721 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
    33781 ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
    33841 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
    33901 cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat
    33961 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
    34021 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
    34081 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac
    34141 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
    34201 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
    34261 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
    34321 cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat
    34381 ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc
    34441 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
    34501 ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc
    34561 tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg
    34621 tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
    34681 ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc
    34741 ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
    34801 ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc
    34861 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg
    34921 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
    34981 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca
    35041 gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg tgtaggcacg
    35101 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
    35161 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
    35221 cagcggcgaa acggtcattg gccagaccca gttgcacaga gtgacggcgt tatgcaaaca
    35281 gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt
    35341 tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag
    35401 tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt
    35461 gaacatacac ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
    35521 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt tacccaaatt
    35581 ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg
    35641 cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca
    35701 gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt tgctgtaaca
    35761 ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacaa
    35821 attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
    35881 cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct
    35941 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
    36001 agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac
    36061 ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt cacccgtttc
    36121 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt
    36181 gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt tgttattcgc
    36241 accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac gatcggcaat
    36301 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccattattgc
    36361 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaataag
    36421 gttttcccca gcctgaccaa tagccgccac acaggtttcc gggctggtca atcgacaaat
    36481 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
    36541 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
    36601 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgcgact
    36661 aaatttcctt tggtaaaagt agaaagtgag gtgatattta cgcgagaact acagggggca
    36721 ccagatccag gtaatgggcc ggtagcaaag actaatttat tcgcttcatc gaaaggtttc
    36781 gtgcctagcg gtacttcgtc atacataatt ttgtagccga agcccatgcc accgacaaaa
    36841 cttttaaact tactggaatc ttcgagggta atatttcctg tcgtgagatt gactcttaat
    36901 atattacctg tccaaccgtt agccatgatt ttttcctttg caagattcac acagtaatat
    36961 ctttccactc gataattttt aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata
    37021 acacacattt cgaggatttt ttactttcgg tatttacggt ggccatcatc cacgggcatg
    37081 ccgtagtaca ggcgctacag ccaatacaac gtttatgatc gacggtaata cagccttctt
    37141 tctgctgcca ggtaatcgca ccaatcgggc agacgttcat gcattgcggc tctttgcatt
    37201 gacggcaggt gtccgcggta tagttgagat cgccatacag gccgccgcca gagccaaccc
    37261 cgttgtcgcc aaagaaataa ttgcgatgga ttttgatacg ggagaagaat gtccctactg
    37321 agccatcgtt gaagttggta caagagattt cacagcggtg gcaacccgtg catcgcgctc
    37381 gctgggtcac cagcacccct ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat
    37441 cttcctgttt gcaacccaaa agtgacagta acgcaggggc aattgttaga ccagcaaggc
    37501 cttttcctga tatacgcaag aattctaatc gcgttaagcc aatatctaat agtggacgat
    37561 caaccgggtt catttatttc gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc
    37621 tgatattaag cattcaacta atacgctgct gactctgtcc ccaacaccag atattttccc
    37681 taacccgata gtgtatgaga aaagatttaa cgccgataaa ctaatccttt atggttagga
    37741 gaaaaaataa tgtgattatt ctgagccctt aacattgatc gttatcaatt aaaattacaa
    37801 agcaagaaat tataagcgaa ctgatattta ttatcatttg aaataaattt aacttaagcg
    37861 acaatcattt gtgacgttca ttgcagaaaa taaaaataac attatcgcta taaattaaaa
    37921 tacataccga aattcgtata atttcagcag ttgttattta gaatgattaa ctggaccgca
    37981 actgaagcaa gaatatcagg atgaagctta ccgcctcatc ctgaatggtc attttaccag
    38041 tgcgttaatg acggtttcca tcgcctgctt ttccagctca ctgcgctgaa tgcgtttatt
    38101 ggctaatgct
//
LOCUS       DAJTRW010000030        37098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRW010000030 DAJTRW010000000
VERSION     DAJTRW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(116..1000)
                     /gene="yhdJ"
                     /locus_tag="OO326_001673"
     CDS             complement(116..1000)
                     /gene="yhdJ"
                     /locus_tag="OO326_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5966086.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1086..1382)
                     /gene="fis"
                     /locus_tag="OO326_001674"
     CDS             complement(1086..1382)
                     /gene="fis"
                     /locus_tag="OO326_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5966087.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1408..2373)
                     /gene="dusB"
                     /locus_tag="OO326_001675"
     CDS             complement(1408..2373)
                     /gene="dusB"
                     /locus_tag="OO326_001675"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5966088.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2702..3583)
                     /gene="prmA"
                     /locus_tag="OO326_001676"
     CDS             complement(2702..3583)
                     /gene="prmA"
                     /locus_tag="OO326_001676"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5966089.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3595..5046)
                     /gene="panF"
                     /locus_tag="OO326_001677"
     CDS             complement(3595..5046)
                     /gene="panF"
                     /locus_tag="OO326_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5966090.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5036..5278)
                     /locus_tag="OO326_001678"
     CDS             complement(5036..5278)
                     /locus_tag="OO326_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5966091.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5387..6736)
                     /gene="accC"
                     /locus_tag="OO326_001679"
     CDS             complement(5387..6736)
                     /gene="accC"
                     /locus_tag="OO326_001679"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5966092.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6747..7217)
                     /gene="accB"
                     /locus_tag="OO326_001680"
                     /gene_synonym="fabE"
     CDS             complement(6747..7217)
                     /gene="accB"
                     /locus_tag="OO326_001680"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5966093.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8195..9169)
                     /locus_tag="OO326_001681"
     CDS             complement(8195..9169)
                     /locus_tag="OO326_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5966094.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9321..11261
                     /gene="csrD"
                     /locus_tag="OO326_001682"
     CDS             9321..11261
                     /gene="csrD"
                     /locus_tag="OO326_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5966095.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11566..12609
                     /gene="mreB"
                     /locus_tag="OO326_001683"
     CDS             11566..12609
                     /gene="mreB"
                     /locus_tag="OO326_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5966096.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12675..13778
                     /gene="mreC"
                     /locus_tag="OO326_001684"
     CDS             12675..13778
                     /gene="mreC"
                     /locus_tag="OO326_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5966097.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13778..14266
                     /gene="mreD"
                     /locus_tag="OO326_001685"
     CDS             13778..14266
                     /gene="mreD"
                     /locus_tag="OO326_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5966098.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14275..14868
                     /gene="yhdE"
                     /locus_tag="OO326_001686"
     CDS             14275..14868
                     /gene="yhdE"
                     /locus_tag="OO326_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5966099.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14858..16327
                     /gene="rng"
                     /locus_tag="OO326_001687"
     CDS             14858..16327
                     /gene="rng"
                     /locus_tag="OO326_001687"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5966100.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16395..20195
                     /gene="yhdP"
                     /locus_tag="OO326_001688"
     CDS             16395..20195
                     /gene="yhdP"
                     /locus_tag="OO326_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5966101.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20351..21796
                     /gene="tldD"
                     /locus_tag="OO326_001689"
     CDS             20351..21796
                     /gene="tldD"
                     /locus_tag="OO326_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5966102.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21924..22853)
                     /gene="aaeR"
                     /locus_tag="OO326_001690"
     CDS             complement(21924..22853)
                     /gene="aaeR"
                     /locus_tag="OO326_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5966103.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23036..23239
                     /locus_tag="OO326_001691"
     CDS             23036..23239
                     /locus_tag="OO326_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5966104.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23247..24179
                     /gene="aaeA"
                     /locus_tag="OO326_001692"
     CDS             23247..24179
                     /gene="aaeA"
                     /locus_tag="OO326_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5966105.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24185..26152
                     /gene="aaeB"
                     /locus_tag="OO326_001693"
     CDS             24185..26152
                     /gene="aaeB"
                     /locus_tag="OO326_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5966106.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26244..26516
                     /locus_tag="OO326_001694"
     CDS             26244..26516
                     /locus_tag="OO326_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5966107.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26572..26835)
                     /gene="yhcN"
                     /locus_tag="OO326_001695"
     CDS             complement(26572..26835)
                     /gene="yhcN"
                     /locus_tag="OO326_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5966108.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27200..27670)
                     /gene="argR"
                     /locus_tag="OO326_001696"
     CDS             complement(27200..27670)
                     /gene="argR"
                     /locus_tag="OO326_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5966109.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28105..29043
                     /gene="mdh"
                     /locus_tag="OO326_001697"
     CDS             28105..29043
                     /gene="mdh"
                     /locus_tag="OO326_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5966110.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29105..30172)
                     /gene="degS"
                     /locus_tag="OO326_001698"
     CDS             complement(29105..30172)
                     /gene="degS"
                     /locus_tag="OO326_001698"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5966111.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30262..31609)
                     /gene="degQ"
                     /locus_tag="OO326_001699"
                     /pseudo
     CDS             complement(30262..31609)
                     /gene="degQ"
                     /locus_tag="OO326_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31763..32161)
                     /locus_tag="OO326_001700"
     CDS             complement(31763..32161)
                     /locus_tag="OO326_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5966112.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32355..33482
                     /gene="zapE"
                     /locus_tag="OO326_001701"
     CDS             32355..33482
                     /gene="zapE"
                     /locus_tag="OO326_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5966113.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33701..34129
                     /gene="rplM"
                     /locus_tag="OO326_001702"
     CDS             33701..34129
                     /gene="rplM"
                     /locus_tag="OO326_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5966114.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34145..34537
                     /gene="rpsI"
                     /locus_tag="OO326_001703"
     CDS             34145..34537
                     /gene="rpsI"
                     /locus_tag="OO326_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5966115.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34932..35570
                     /gene="sspA"
                     /locus_tag="OO326_001704"
     CDS             34932..35570
                     /gene="sspA"
                     /locus_tag="OO326_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5966116.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35576..36073
                     /gene="sspB"
                     /locus_tag="OO326_001705"
     CDS             35576..36073
                     /gene="sspB"
                     /locus_tag="OO326_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5966117.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36116..>37098)
                     /gene="dcuC"
                     /locus_tag="OO326_001706"
     CDS             complement(36116..>37098)
                     /gene="dcuC"
                     /locus_tag="OO326_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5966118.1"
                     /translation="FITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGI
                     LETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQA
                     EQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEF
                     FRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSG
                     LGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATT
                     LARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8966 a         9280 c         9781 g         9071 t
ORIGIN      
        1 aaaacgacga gccaccaata cttgcgaatc attacctgag cctgtgcatg tttaaccaag
       61 tctggcagga aatatacacg aaatgaataa tctgaaaact aggcttacac ttcctttact
      121 ttgcaatgag atcggggtca acttcgctta accggcttcg ttttgacagg ttgccggtct
      181 ttcttttttt cactttcgcc agttcttccg cggagtaatg cgacgcgaca tccagccgtc
      241 gaagccccat tttgatgtac tcgctgttga tctcaatacc aatgaatttt cgtccgctgg
      301 cgatggctac ggcaccggta gtaaagctac cggcaaacgg gtcgagaacg atatcgcctg
      361 ggttggaaga ggcgagaata atgcgtttca gtaaggcttt cggtttttgc gtcgggtggt
      421 tctcatattc atccatcaaa taacgcacgc gcggaaaatc ccagacgtta cccggtactt
      481 tttgatgatt gtatggctgt ggaggatttt tgcgataatc gatcaacgcg cgctgcgatc
      541 cggttttggc ttctaccaga atagcatcac cgttgaatgt gtagttcttt gcgtctttca
      601 ccatcatcag gatgggttcg tacatggagc cgtagtgttt tttcgcctgt actccagaac
      661 tgtcatatga ccagacgatg cgacttttga tggtaaaaag cttgcggcac tggagatcga
      721 taaagggcat gttttccgta ctgttcataa tgtacatgct gccctgcttt ttcagaacgc
      781 ggtggcactc tgcaatcact tcaaacagcc agtcgataaa cagatcttct ttccaggctt
      841 cgatcagacc atcaaaattt ttaccgatgt tatacggtgg gtcggcaaag agcagatcga
      901 cgctttcagt aggtagcttt ttaagttcgg caaaggcatc accgtgaata atggtcttag
      961 cttcattacc aaaccgggtc ggttcacatc ctgttctcat ggtcactccc tttgtgacac
     1021 ctataaaaaa ggcgcttccc catgccgagt agcgcctttt taatcaagca tttagctaac
     1081 ctgaattagt tcatgccgta ttttttcaat tttttacgca gcgtaccacg gttgatgccc
     1141 atcatcagcg cagcacgggt ctggttacca cgggtgtatt gcatcaccat gtccaacagg
     1201 ggctgttcta cttcagccag taccagctca tagaggtcat tcacatcctg accattcagt
     1261 tgagcaaaat agttcttcag tgcctgttta accgagtcac gcaggggttt ttgggttacc
     1321 tgatcctgag agttaacggt agaaacggtc agtacgtcag aatttacgcg ttgttcgaac
     1381 atagttctgt cagctcttta tttctgttta cgcaaaattt tcgaagtatg cctccaacgc
     1441 ctccagctgt tcgctggcat cctcaatggc gttgaatgtg cgccgaaact ggtcatttgg
     1501 agcgtgttcc tggagatacc aggaaacgtg tttacgtgca attcggtacc cttttgccgg
     1561 accataaaag tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa cctctgccaa
     1621 aggcagtggg ggcagcaact ccccagtgtc cagataatgc tggatttccc gaaagatcca
     1681 gggtcttccc tgagctgcgc ggcctatcat cagggcatcc gcccctgtat agtcgagcac
     1741 agctctggct ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa tggaaacttt
     1801 ctgcttaact gcccgaatac tgtcgtactc agcttctcca ttgaacaaac aggcgcgtgt
     1861 acggccatga atggtcagag cctgaatgcc acagtcttca gccagttggg caatctcttc
     1921 gcagttacgg tgttccggtg cccagccggt gcgaatcttc agggtaacag gaacgtccac
     1981 tgcattgacg acctcggtaa ggatcgattt aacgacatcc gggtactgca agagggctga
     2041 acctgcgagc ttgcgattca cttttttagc cgggcaaccc atattgatat caataatctg
     2101 ggcaccgctt tccacgttaa tacgtgctgc atctgccatt tctttcggag cgctaccagc
     2161 aatttgcacg gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac gagatttgtc
     2221 gctttcccaa acctgtgggt tagaagacat catctcggat actgtcaatc cggctcccat
     2281 ctcgtagcac aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg ctgcgatcag
     2341 gcgatttctg agctgatatt gtccgatgcg catgagttaa gaaatgacca tactgtgact
     2401 gcaaggcggc gtatattacg cattttttgc acgagatgaa aggccaaact ttgaccaatc
     2461 ctctgagatg gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat tactcataaa
     2521 aatcatcata ttagaaaata gtgactaaaa tttacactca aagaaatttg agtaagttct
     2581 caatttttct ttatgaatga aaattttggc acgcaaattt gcgtaaataa tcggcaaatt
     2641 tacgtgcctt ttgtgagctt gctcgcactt cgccccgcgt caccctacgg cgatgcgaag
     2701 gtcaattctt acgaccggta atacggcacc actcttcttt ttccacgacc gggtccagtg
     2761 cgaagctatc ggcataagct tcacaaacgc tctctgcctg gcttgccaga ataccggaaa
     2821 ggcccagcaa accgcctgaa actggcagga cgctgattaa cggtgccagt tcacgtaatg
     2881 ggcctgcaag gatgttagcg accaccacgt cggctttcat ttcttctggc tgatctttcg
     2941 gtaagtagag ttccagacgg tcagaaacgc cattacgttc ggcgttatcg cggctggcct
     3001 gaatcgcctg cggatcgata tcaataccaa tggcttttgc tgcacccagt ttcagcgccg
     3061 cgatcgccag aatgccggaa ccacagccaa agtcgatgac tgttttaccg gttaaatcga
     3121 ggctgtcgag ccattgcagg cacagagagg tggttggatg ggtacccgta ccaaacgcca
     3181 gccctggatc taacatcacg ttgacggcgt tttcgtccgg cacatcacgc cagctagggc
     3241 agatccacag tcgttcacca aagcgcatcg ggtggaaatt atccatccat tcgcgctccc
     3301 agtctttatc ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc agcggatggt
     3361 tttccagaat cgccaccacg tcgttcatat cggtttcagc gtcgaacaga ccaatcacat
     3421 cggtgtcgcc ccacaggcgc gtttcgcccg gcagcggctc aaatactggc gtatcgtggg
     3481 tatcctgaaa agtgatagaa acggcacccg cttccatcag cgcatcgcta agatcttccg
     3541 cgttcgcgcc ggtggtgttc agtttcagtt ggatccaagg catggcaaaa ctctttattt
     3601 atcagtagtc aaaacggtag cttgcgggac ggatgtaccg aaacggtttc cgaccaggaa
     3661 agccagcaaa cttagtagta acgagggcac gatagggtgg aagcccaggt actgaatatt
     3721 cagcgtcgcg agtacggcat acagcacgcc gccaacgatc atcgcactta gcgcgccttt
     3781 ggcgttggcg cgttcccagt aaagacccag caccagcggc cacaggaaaa cggcttccag
     3841 cccaccgaag gccagcaaat tcagccagat gatcatttct ggcggcttcc aggcggcaag
     3901 cagcagcaac gcgccgagaa ctaacgtaat taccgccgac atccgcttca gacgcgtctc
     3961 gttttgcatt tgatccggac ggatattcag atagagatct ttaatgatcg tagcggaact
     4021 ttgcagcagt tgggcgttaa ttgtcgacat gatcgcagcc ataggtgcag ccagaaagat
     4081 cccggcagca aaaggtggca gcacttttac cattaacgtc gggatcacca gatctggtac
     4141 ggtgagatcg gggatcactg cacgacctaa cgctccggcc aggtgcatac cgaacatcag
     4201 gattgcgact ataatcgtac cgatgatgat cccccggtgt actgctttgc tgtctttata
     4261 agagatacag cgcaccgcag tatgcggcag gccaatcacg ccaaagcaca ccagtaccca
     4321 gaacgacgtc ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg taaccagttg
     4381 cggatcgatg gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg catgtaccac
     4441 gccaataagc agcacaacgg tgccaatcag catcacaagc ccttgcatgg tgtcgttcag
     4501 cacgctggcg cgaaagccac caaaggcggt atataacgca atgctgatac caaaaatcag
     4561 cagcccggtt tcataaggaa tacccgccgc ggtttccagc aggcgcgcac cgccgataaa
     4621 ctgcacggtc atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca gccacaccag
     4681 aagacgactc tggtagcggg caaacagcat atcgttcagc gtcactgcat tgtagcggcg
     4741 cgcaagaatc gcaaacttct tgccgagaat accgagtgaa agccagacag caggaagctg
     4801 aatcatcgcc agcaataccc agcccagccc gtatttataa gcagctcctg gcccgccgat
     4861 aaacgaactg gcactgatat aggtcgcggt gagagtcatc gccagcacaa taccgcccat
     4921 agagcggctg ccgaggaaat actcattaag gaaggtgccg gtgctccgtt tacgcatcgc
     4981 ataaaccgag ataccgaaca ccaccaccag ataggcgacc agcggtagaa ttacttcaag
     5041 ctgcatcgtc atcctccagt gggatatcgc gatagataaa tttcaccatc gcccagcaca
     5101 gtccaataaa cagtagcggc gtcaggatgc aggccatctc aaaccagcgc ggaaagccgg
     5161 taaaaccggg ggcaacgcca ggtaagtaag cgtctactaa ccaaactgcc agatacaaaa
     5221 gggtcagccc cagcgcccag cgggcctctt tatgggcctg aacaaaacga gtgtccattt
     5281 tttgtccctg atgggtgaag aaagcgagga ttgtacctta tgggggttgt cgatccccag
     5341 taataaaaaa ggccggaaaa tccggccttt tgacgctttt gcagtcttat ttttcctgaa
     5401 gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat
     5461 tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc agctcctgca
     5521 gcgcattctt catgcgggca atcgccacgt cacggttttc accgtagcaa atcagcttac
     5581 cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac
     5641 gtacgccaaa accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg
     5701 tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt
     5761 cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa
     5821 cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc atttcgatga
     5881 aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat
     5941 caacacacgc tttagcgcaa cgttcgccga tgtagcgacg cagttccggg gtaatgcccg
     6001 gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag tcacgttccg
     6061 ccagatagat agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat
     6121 tctccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc gcttccgcac
     6181 gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc ataccgcgac
     6241 cgccgccgcc gccggaggct ttgataatca ccggataacc aatgcgttta gcaatggcgc
     6301 ggtttttatc catatcgtcg cccagcgggc cgtcagaacc aggtacgcaa ggaacgcccg
     6361 cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg gtttctgctt
     6421 tcgggccgat gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg
     6481 agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgctgatga
     6541 ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat
     6601 ctgccagtaa tacgtgtttt agatcgcgat ccgtgctgga gtgcacagcg acagtcttga
     6661 tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa
     6721 caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct
     6781 accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc
     6841 tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga
     6901 cccacttcga tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc
     6961 ggggaacgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg
     7021 gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct
     7081 tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac tgactcttcg
     7141 ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta
     7201 atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg
     7261 tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag
     7321 caggccggga cgccggtcta ttttgcccca gagtgtcaat gttagacttg acggacattg
     7381 tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt
     7441 caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat tataacgatt
     7501 tcgtagcaat tggcagctaa atactggtct tatcagggaa tataatcaac agctaacatg
     7561 taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc
     7621 atcatcgacg gaacttctta taacgtaagg tgaaaagctg aaaagccagc cctgcgaaga
     7681 tgaggggctg caatgagata atcttcacag accacaaata atgtagggat gccaggatcg
     7741 cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt cactgcatcg
     7801 tctggataga tgtgaatgct aaagcacgca aaatcaacca actgacaatt ctcagcatta
     7861 aataggttcg agtggttaat ttgtagtcaa ataaaatcag attattcacc ccatggtacc
     7921 agaaatcagt aacaggttaa gcgtagtgtc gcccaggcaa agcaccataa acctaacagg
     7981 cgacggaggc gtatcaataa tggctgttta gaggagtatc aatgcgcatc aatttgtggt
     8041 cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcctga ccaaggtgtt
     8101 cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat gatgtcaaaa
     8161 tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc agcgtgcgac
     8221 cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct
     8281 cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt
     8341 gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca
     8401 gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca caggccgcca
     8461 cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt
     8521 caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag
     8581 ggagaatacc gctggcacct aaacttttca gatattcatg ggtactttcg cgaccggaaa
     8641 cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac
     8701 cactggcacc cgtcacgaca atctcaccgt cctgcgggcg aacaccggca tcttccagcg
     8761 ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aatcattgct ttacgcgcgt
     8821 ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc
     8881 caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga ccggcatgaa
     8941 aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa
     9001 aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg
     9061 accagtgaac atcgaccgtg acatcgccct ccggcaggcg acgttcgtcc agagtctgta
     9121 ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc
     9181 atgtgcatga tggattggaa aataatttct gaagactata ctcgctaatg gaaaagcaaa
     9241 gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca
     9301 ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc
     9361 tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcca
     9421 ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg
     9481 tgtcgaatga cttcagcgca ttaaggccac aaattaccga attaatggtg tcagcagata
     9541 tcgttcgtgt agacctgctg catggtgata aacaggttta taccctggcc agaaatggta
     9601 gttatcgtcc ggttggcacc agcgatctgt ttcgtgaact gagcgttccg ttgataaagc
     9661 atccggggat gtcgttgcgt ctggtttatc aggatccgat gggcaactat ttccattcgt
     9721 tgatgaccac cgcgccgctc acgggggcga ttggctttat cattcttatg ctctttctgg
     9781 cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc
     9841 gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca
     9901 gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca
     9961 gccgtcttga tacgctgatc cgctcttatg ccgcacagga cgtgaaaacc ggcctcaata
    10021 accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta
    10081 cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacctggg
    10141 ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga
    10201 tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac
    10261 cgcaccggac gttaaaagag gcagagagca tagccagtca gttaatcaaa gccgtcgata
    10321 ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgtgcct
    10381 ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg
    10441 cgggattgca gggtggcaat agctgggcta tttatgatga ctcgttgcct gaaaaaggac
    10501 gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc
    10561 tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc
    10621 gcatcttcga tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt
    10681 ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt
    10741 actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgtccgcgtt
    10801 ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa
    10861 ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc
    10921 gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa
    10981 gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca
    11041 ggaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct
    11101 ccgggaccag cacccacgtt tacgccaccg gcgtgcgttc gcgaagcgag tggcagaccc
    11161 tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg
    11221 atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt taatccgttt
    11281 tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt
    11341 tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg
    11401 atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta
    11461 attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta
    11521 agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt
    11581 cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat
    11641 gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt
    11701 gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt
    11761 acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc
    11821 gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacag ctttatgcgt
    11881 ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca
    11941 attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg
    12001 gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat
    12061 atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct
    12121 tccgtgcgca ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat
    12181 tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct
    12241 tacccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt
    12301 ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc
    12361 ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac
    12421 atctccgagc gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt
    12481 ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg
    12541 gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc
    12601 gaagagtaat cggatgcagg caggggaagc gtctgtttac cctgcctggt ctgatacgag
    12661 aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta
    12721 ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca
    12781 gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc
    12841 ctcgtgaatt gctggatggc atatcgcaga cgctggcctc gcgtgaccaa ctagaacttg
    12901 aaaaccgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac
    12961 aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg
    13021 agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg
    13081 ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag
    13141 gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg
    13201 atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg
    13261 gtaacggttg tacggatgat ttgcagcttg agcatctgcc agcgaatacg gatattcgtg
    13321 ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc tatccggtcg
    13381 cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatc caggcgcgtc
    13441 cgactgcagg actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg
    13501 gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga
    13561 tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccggcaa
    13621 cggggatcgc tcagccgact ccgcagcaac cggcgacagg aaatgcagct actgcgcctg
    13681 ttgcgccgac acagcccgct gctaatcgct ctccacaaag ggctacgccg ccgcaaagtg
    13741 gtgctcaacc gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc gtagccaggg
    13801 acgctgggta atctggctct ctttcctcat tgcgctgttg ctgcaaatca tgccctggcc
    13861 ggataacctg attgttttcc ggccaaactg ggtgttactt atcttgttgt attggatcct
    13921 ggccttgccc catcgcgtaa atgtgggcac aggttttgtg atgggtgcca tactggatct
    13981 gatcagcggc tcgatgcttg gcgtacgcgt attggcgatg agcatcattg cttacctggt
    14041 ggcgctgaaa taccagcttt tccgcaacct cgcattatgg cagcaggcgc tggtcgtcat
    14101 gttgctttcg ctggtggtgg atattattgt tttctgggca gagtttttag tgattaacgt
    14161 ctctttcaga ccggaagtgt tctggagtag tgtagtcaat ggggtgctct ggccgtggat
    14221 tttcttgctg atgcgtaaag tccgtcagca gtttgcagtg caataaaggt ttctatgact
    14281 tctctgtatt tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg
    14341 acctttgaac gtattgttac gggcattgag gagcagcgtc agccgcagga gagcgcgcag
    14401 cagtatgttg tgcgtctggc acgcgagaaa gcacaggcgg gtgtcgcgca aacggcacag
    14461 gatctcccgg tgctgggtgc ggatattatc gttatcctga acggagaagt gctcgagaaa
    14521 ccgcgcgacg cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag
    14581 gtgatgacgg cagtggcgtt ggccgacagc cagcacattc tcgattgcct ggtggtcacc
    14641 gatgtgactt tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc cagcggtgaa
    14701 ccgttagata aagcaggtgc atacggtatt caggggctgg gtggctgttt tgtcaggaag
    14761 ataaatggca gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta
    14821 agtaatttta acgcactgcg tgagaaaagg gataaacatg acggctgaat tgttagtaaa
    14881 cgtaacgcct tcggaaacgc gagtggcgta tattgatggc ggtattctgc aggaaattca
    14941 tattgaacgt gaggcgcgac gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg
    15001 tgtacttccg ggtatgcagg cggcttttgt agatattggg ctggataaag ccgcgtttct
    15061 tcatgcatcc gacatcatgc cgcataccga atgtgtggcg ggtgaagaac aaaagcaatt
    15121 cacggtgcgc gacatctcgg aactggttcg tcaggggcaa gatctgatgg tgcaggtggt
    15181 gaaagatccg cttggcacta aaggtgcgcg cctgaccacc gatatcacgc tgccttctcg
    15241 ctatctggta tttatgccag gggcttctca cgttggggtt tcccaacgaa ttgaaagcga
    15301 atcagaacgt gaacgtctga aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt
    15361 tatcatccgc accgcagcgg aaggggttgg cgaggctgaa ctggcctccg atgccgctta
    15421 tctgaaacgc gtctggacca aagttatgga gcgtaaaaag cgcccgcaga cccgttatca
    15481 gctgtacggc gaactggcgc tggcgcagcg tgttctgcgt gattttgccg acgccgaact
    15541 ggaccgcatt cgcgttgact cacgcctgac ttacgaagca ttgctggagt ttacctcgga
    15601 gtacattccc gagatgacca gcaagctgga gcattacacc ggacgccagc cgattttcga
    15661 tctctttgat gtcgaaaacg aaatccagcg agcgttggaa cgcaaagtag aactgaaatc
    15721 cggtggttat ctgattatcg accagaccga agcgatgacc accgtggaca tcaataccgg
    15781 agcgtttgtc ggtcatcgca atctggacga cactattttc aataccaata ttgaagcgac
    15841 gcaggctatc gctcgccagt tacggttgcg taatctgggc gggattatca ttattgattt
    15901 catcgatatg aataatgaag atcaccgccg ccgcgtgctg cattcgctgg agcaggcgtt
    15961 gagcaaagac cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc tggtggagat
    16021 gacgcgtaaa cgcacccgcg aaagcattga gcacgtactg tgtaacgaat gcccaacctg
    16081 ccacggtcgc ggaacggtga aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat
    16141 tgttcgtgtc caccatgctt acgactccga ccgtttcctg gtctatgctt ctccggcagt
    16201 agccgaagcc ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg
    16261 caaacaggtt aaagtacaaa ttgaaccgct ctataaccag gagcagtttg acgtcgtaat
    16321 gatgtaaaca gatgctgggc cgccatccgg caaagggttt ttgagtcaca tttttagcag
    16381 acaaggagtg acgggtgagg cgattgccgg ggattttact gcttactgga gccgcgctcg
    16441 ttgtgatcgc tgccctgctg gttagcggcc tgcgtattgc tttaccgcat cttaacgcct
    16501 ggcgtccgga aatcctcaac aaaatagaat ccgcgactgg catgccggta gaagccagtc
    16561 agctctcagc cagctggcag aattttggcc cgacgcttga agcacacgac atccgtgcag
    16621 aactaaaaga tggcggcgaa ttttcggtta aacgcgttac tctggcgctg gatgtctggc
    16681 agagcctgtt acatatgcgc tggcagtttc gcgacctcac tttctggcag ctgcgctttc
    16741 gcaccaacac tcctatcacc agcggtggta gcgatgacag tctggaagcc agtcacatca
    16801 gcgatctgtt tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa gtcagttttc
    16861 tgacgccatc cggtcagcgc gccgagctgg cgatcccaca actcacctgg ctgaacgatc
    16921 cacgtcgaca ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga cagcacggcg
    16981 tgatgcaggt gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct
    17041 ggctacaggc ggatgacatc gacctgaagc cgtggctcgg taaatggatg caggacaata
    17101 ttgctctgga aacggcacag ttctcccttg aaggctggat gacgatcgac aaaggcgatg
    17161 taaccggcgg tgacgtctgg ctgaaacagg gcggtgccag ctggttgggc gagaagcaaa
    17221 cgcatacgct gtcggtggat aatctgaccg cgcatattac gcgtgaaaat ccgggctggc
    17281 agttctctat tcccgataca cggatcacga tggacggcaa accctggccg agcggagcat
    17341 tgacgctggc ctggataccg gaacaggacg ttggcggcaa agacaataaa cgcagtgacg
    17401 aactccggat tcgcgctagt aatctggagc tggcaggcct ggagggcata cgcccgctgg
    17461 ccgcgaaact ttcacctgca ctgggtgatg tttggcgctc cacacaaccg agcggcaaga
    17521 ttaacactct ggcgctggat atcccgcttc aggcggcaga caagacccgt tttcaggcat
    17581 cgtggagcga tctggcctgg aagcaatgga aattattacc gggtgcggaa cacttctccg
    17641 ggacgctttc cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga
    17701 tgccttacga aacggtattc cgtgcgccac tggaaatcgc cgacggccag gcaactataa
    17761 gctggctgaa caatgacaaa ggtttccagc tggatgggcg taatattgac gttaaagcca
    17821 aagccgtcca tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat gaaccctggc
    17881 tgggtattct ggctggcatc agtaccgatg atggttcaca agcctggcgc tatttcccgg
    17941 aaaacttgat gggtaaagac ctggttgatt atttaagtgg tgcgattcag ggcggtgaag
    18001 cggataacgc gacgctggtt tatggtggca atccgcaact cttcccctat aaacacaacg
    18061 aaggtcagtt tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc cagccggact
    18121 ggcctgcatt aactaacctt gatattgaac tggactttat taacgacggt ttatggatga
    18181 aaaccgatgg cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg
    18241 actactcaaa agaaaaactg ctgattgacg ctgacattaa aggtccgggt aaagccgttg
    18301 gcccttactt tgatgagaca ccgctgaaag attctctggg tgcgaccctg caagaactcc
    18361 agctcgacgg cgatgtgaat gctcgcttac atcttgatat cccgctgaac ggcgaactgg
    18421 taaccgcgaa aggtgaagtg acgctgcgta ataacagtct gtttatcaaa ccactcgaca
    18481 gcatcctgaa aaatttgagc ggtaaattca gctttatcaa tggcgatctg caaagtgaac
    18541 cactgacagc aagctggttt aatcagccgt tgaacgtgga tttttccacc aaagaagggg
    18601 caaaagccta ccaggttgcg gtgaatctca acggtaactg gcaaccggcg aaaaccggcg
    18661 ttctgcctga agcggtgaac gaagcattga gtggcagcgt ggcgtgggat ggtaaagtgg
    18721 gcattgatct gccttatcat gctggcgcga cctataacgt agagctgaac ggcgatctga
    18781 ataatgtgag tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa ccactagcgg
    18841 taaacgttaa ggttgatggc aatctcaaca gctttgaatt aaccggacag gctggtgcgg
    18901 ataaccattt caatagccgc tggttgcttg gtcaaaagct gacgctcgat cgtgctattt
    18961 gggcggcaga cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt gaacttaata
    19021 tgccgccgat gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct gcggagagtg
    19081 tcggtggtgc agcgagtttc ccacaacaca taacgttacg tacgcctatg ttgtcactgg
    19141 gaaatcagca atggaataac ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg
    19201 ttgaggcgca agggcgtgaa atcaacgcca cgctagcgat gcgtaataac gcgccgtggc
    19261 tggcgaatat caaatatctt tattacaacc cgagcgtggc gaaaactcgt ggtgattcaa
    19321 cgccgtcatc acctttcccg acaacggagc gcattaactt ccgtggctgg ccggacgcac
    19381 aaatacgatg cacagagtgc tggttctggg ggcaaaaatt cggtcgcatt gacagtgata
    19441 tcaccatttc tggcgatacg ttaacgctga ccaatggact gattgatact ggtttctcgc
    19501 ggcttactgc cgatggtgaa tgggttaata atccggggaa tgaacgtacc tcgctgaaag
    19561 gaaaactgcg tgggcagaaa attgatgccg ccgcagaatt ttttggtgtc acgacgccca
    19621 tacgccagtc gtcatttaat gtggattacg atttacactg gcgcaaagca ccctggcagc
    19681 cagatgaagc gacgttgaat ggcatcattc atactcaact gggtaaaggc gaaattaccg
    19741 aaatcaatac cggacatgcc gggcaattgc tgcgcttatt gagcgtagat gccctgatgc
    19801 gtaagctgcg ttttgatttc agagacactt ttggcgaagg gttctatttt gactccattc
    19861 gcagcaccgc gtggattaaa gacggcgtta tgcacaccga cgacacgctg gtggatggcc
    19921 tggaggcgga tatcgccatg aaagggtcgg taaatctggt acgccgcgac ctgaatatgg
    19981 aagcggttgt cgcaccagag atttctgcga cggtgggcgt ggctgcggct tttgcggtta
    20041 accccattgt tggcgcggca gtgtttgccg ccagtaaagt gctggggccg ctgtggagca
    20101 aagtctccat tttgcgctat cacatttcgg gtccgctgga cgatccgcaa atcaacgaag
    20161 tgttgcgcca accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg taattgcccc
    20221 aatctcatat gataatcgtt gccaaaggcc aacgagccgg aacataactg taggccggat
    20281 agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta actaacgagt
    20341 agcaaaaacg atgagtctga acctggtaag tgaacaattg ctagcggcga acggcctgaa
    20401 acatcaggac ttgttcgcga tcctcggtca actggccgaa cgtcgccttg attatggcga
    20461 tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca ttattaaaga
    20521 tggttcttac aacatcgatc agggcgttgg cgtgcgtgca atcagcggtg aaaaaaccgg
    20581 atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc aagcggcgcg
    20641 caccatcgtc cgtgatagtg gtgatggcaa agtacagacg ctgggcgcgg tagagcatag
    20701 cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga agctggatat
    20761 cctgcgtcgc gtcgataagg ttgctcgcga agcggacaag cgcgtacagg aagtgactgc
    20821 cagcctcagt ggtgtctatg aattaattct ggttgcggcc accgacggca cgctagcggc
    20881 ggatgtccgt ccgctggtgc gtctttccgt gagcgttctg gtcgaagaag atggcaaacg
    20941 cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gagttcttcc ttgccgatct
    21001 tgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg cgctggtcaa
    21061 tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg caggttggcc
    21121 gggcgtgctg ttgcatgaag cggtaggtca cggtctggaa ggcgacttca accgccgtgg
    21181 cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt gtaccgtggt
    21241 tgatgacggc acgatggtcg atcgccgtgg ttcggtggcg attgatgatg aaggtacgcc
    21301 aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc aggataaact
    21361 caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat cctacgccca
    21421 tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga ccccgcaaga
    21481 aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg gtcaggtgga
    21541 tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg aaaacggtaa
    21601 agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa ccatgcagca
    21661 gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct gcggtaaaga
    21721 agggcaaagt ttgccggttg gcgtgggcca gccaacgttg aaagtcgata acctgactgt
    21781 tggcggtact gcgtaataat ttcattattt tcagaaggat aattaatctt tctacgtgca
    21841 cgaacggtcc cctcgctcct ctggggttag ggtgagggga acccgttggc acaggtttgt
    21901 agaacgtaac agtacaatat gaattacttc tctttcccgc gcccgtgcat ctcctgaaac
    21961 aatttaccga cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc
    22021 ggcagcttat ctttttcggt atataacgca taaaccgggc gtggatctga ctgataacgc
    22081 ggcagcagga tctccagctc cccacgattg atctcgttga tcacccacat cagcggcacg
    22141 taggcgatcc cggcacccgc cgttagccag cgcaccagcg tcatcggatc attagtcaca
    22201 aatctccctt gcgggatcag gcgagtcgag atcccttccg gtgcaatcag ttcaaattca
    22261 ttgtcgggcc gcacgctgta ttcaagccat gaatggctac ttaaatcggc gggtttttcc
    22321 ggtatgccgt attgtgtgag atagcttttc gcggcgcaca ccaccattgg catcgcgcca
    22381 agacggcggg aaaacaggct ggaatcctgc aacgcgccga cgcggatcac cacatccaga
    22441 ccgtcggcaa tcaggtcggg ggctggaatt ccggtaacca gattggcgct caaacctggg
    22501 tattctttca gcattttggc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa
    22561 cagccaatgc gtagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca
    22621 tcctgcactt catgaagcat acgacggcag ccctggtagt aaattctacc ggcttcggtc
    22681 aggccaatgc tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt
    22741 gacaccgtct gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa
    22801 gagccaaatt caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac
    22861 tctgacttaa aagtgattta gatcacataa tatagataac agcataacag ttacgctaat
    22921 atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc
    22981 ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag
    23041 tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct
    23101 tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga
    23161 ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat
    23221 atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg
    23281 ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt
    23341 acaccgaatc cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc
    23401 cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag
    23461 gacagatact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag
    23521 ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta
    23581 accgtctcgg tgtgcaggcg atgtctcgcg aagagatcga ccaggctaac aacgtactac
    23641 aaacggttct gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc
    23701 ttgaacgcac ggtgatccga gcgccagcag atggctgggt gaccaacctc aacgtctata
    23761 ccggtgagtt tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct
    23821 atgtactggc ctatatggaa gaaactaagc tggaaggggt gcgtccgggg tatcgtgcag
    23881 agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgctg
    23941 gggtcaccaa cgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctgacc
    24001 ttgaatgggt gcgtttggcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag
    24061 agaacatctg gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg
    24121 acgaaagcca ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat
    24181 cacgatgggt attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac
    24241 cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc
    24301 ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta
    24361 ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat
    24421 tgccggactg gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg
    24481 ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta
    24541 cgcgtggggg ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc
    24601 attgcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg
    24661 tgcgattatg gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tagatcgaga
    24721 gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga
    24781 tggtgaagtt gtcgataaag cctggggcga cctggtgcga cgcaccacgg cactacaagg
    24841 catgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa
    24901 agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca
    24961 gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga
    25021 aaccgcgcag gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggactgg
    25081 ggaacgggaa acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct
    25141 tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca
    25201 aggcgaaccg gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg ttaacttctg
    25261 gcgaaccaca ctttcctgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc
    25321 cggcagtggt gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa
    25381 tccacgcatg gtggcgatcg actttatcta tgggacgctg gccgcgctgc cgttagggct
    25441 gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag
    25501 cctggcagtg ctgggattct tcctcggtat agaagtacag aaacggcgac tgggctcgat
    25561 gggggcactg gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt
    25621 cagtcagttt ctcgacagcg cattaggaca aatcgtcggc tgtgtgctcg cgttcaccgt
    25681 tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt
    25741 tgtttctgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct
    25801 cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa
    25861 atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc
    25921 ggttaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat
    25981 atctgcccgt agcgatgata aacgtcgtcg gtactttggc cagttgctgg aagaactgga
    26041 aatctaccag gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca
    26101 tcggctggcg gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac
    26161 cgacgccaaa agcgtcggtt ttttcatggc tatacttagc gatgcacggc agaactcgcc
    26221 gcgaaacgtg acggtggcaa cagatgaata tttatgcctt tgattttgat gagattgaga
    26281 gtcaggagga tttttatcgt gactttagcc aaacctttgg tctggctaaa gataaggtac
    26341 gcgatctcga ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga
    26401 ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt
    26461 ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca
    26521 aaaaaaagcc cacgaaccgg gggcaatatc gtcggacaaa acgatgaggg tttatttgta
    26581 cagttcagcc gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt
    26641 tgcgcctttc tcttccgctt ttttgttcag catttcacgt atatccattg gcgaagacgc
    26701 cacaccactt acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagcatcaat
    26761 ggagtcggca gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagctacagt
    26821 ggttttgatt ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg
    26881 acccatatca caaaatcaag tatacactaa ttattgaact aattaatacc acactaactg
    26941 tttcagttga tactaagtgt taaaaatgtg aattttaata acaaaaaagt ataaaaaaca
    27001 cggttattta ttattaatta tagtattttc aattagataa tgaattttgc ataatgtgct
    27061 ttttttaccg tatgtattca ttttgtgaat gatgtgtcgc ggtaaaacgc actattcgtt
    27121 aaatgtatcg caggagaaag aaggggaatg agagggataa gcaacatttt ccccgccgcc
    27181 agaagcgacg gggcagagat taaagctcct ggtcgaacag ctctaaaatc gcttcgtaca
    27241 ggtctttgac ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg ccagcgatgg
    27301 tgcccagaat accttctgct ttgcccagtg agtccagcag gcgagcaatt aactgcgccg
    27361 cgccagggct ggtatgaatc acgacaactg catcgttgta gtcgatatcc agcaccagat
    27421 tcttcaatgg actggaggtg gttggtacac ccagttcagc tggcaggcag taaaccattt
    27481 ccattttggc attgcgtgta cgtacagcac caaacttggt caacatccgc gagactttag
    27541 actgattaat attgtcaaag ccttgctcct gcaacgcggc gacgatttcg ccctgggagc
    27601 taaatttctc ttctttaagt aatgctttaa atgctttaac tagttcttct tgcttagccg
    27661 agcttcgcat aagtcacccg atatggtggt tgatacaaca ttattgtgca tacagatgaa
    27721 tttttatgca aacagtcagc cctgaagaag gctgaaataa tgttatgaaa gagcgggatt
    27781 ttatcaaatt tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt ctgtttaaaa
    27841 gtaaattaat tgttatcaaa ttgatgttgt tttggctgaa cggtagggta tattgtcacc
    27901 acctgttgga atgttgcgct aatgcataag cgactgttaa ttacgtaagt taggttcctg
    27961 attacggcaa ttaaatgcat aaacgctaaa cttgcgtgac tacacattct tgagatgtgg
    28021 tcattgtaaa cggcaatttt gtggattaag gtcgcggcag cggagcaaca tatcttagtt
    28081 tatcaatata ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc tggcggtatt
    28141 ggccaggcgc ttgcactact gttaaaaacc caactgcctt caggttcaga actctctctg
    28201 tatgatatcg ctccagtgac tcccggtgtg gccgtcgatc tgagccatat ccctactgct
    28261 gtgaaaatca aaggtttttc tggtgaagat gcgactccgg cgctggaagg cgcagatgtc
    28321 gttcttatct ctgcaggcgt agcgcgtaaa ccgggtatgg atcgttccga cctgtttaac
    28381 gttaacgccg gcatcgtgaa aaacctggta cagcaagttg cgaaaaactg cccgaaagcg
    28441 tgcattggta ttatcactaa cccggttaac accacagttg caattgctgc tgaagtgctg
    28501 aaaaaagccg gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct ggatatcatt
    28561 cgttccaaca cctttgttgc ggaactgaaa ggcaaacagc caggcgaagt tgaagtgccg
    28621 gttattggcg gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt tcctggcgtt
    28681 agttttaccg agcaggaagt ggctgatctg accaaacgta tccagaacgc gggtactgag
    28741 gtggttgaag cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca ggcagctgca
    28801 cgttttggtc tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt cgaatgtgcc
    28861 tacgttgaag gcgacggtca gtacgcccgt ttcttctctc aaccgctgct gctgggtaaa
    28921 aacggcgtgg aagagcgtaa atctatcggt accctgagcg catttgaaca gaacgcgctg
    28981 gaaggtatgc tggatacgct caagaaagat atcgccctgg gcaaagagtt cgttaataag
    29041 taattgatta gcgaataata aaaaaccgga gcacagactc cggtttttgt tttgagcact
    29101 cgacttaatt ggttgccgga tattcctgaa tggtgacctg cagcgttaac tgcttatcat
    29161 cacgcatcac tacaaccggg atcaccgaac cagggcgaat ttccgccacc tgatccatcg
    29221 tctccagagc agagatggcc ggtttgttat ccaccgaaat aatcagatcg ttgacctgaa
    29281 tacccgcatt cgccgctggg ccgtcaggtg acacttcatt aaccacgatc ccttgcagtt
    29341 gatctatacc accgctctgc gcgtgcagtg gtgcgatctc gcgtccgccg ataccaatgt
    29401 agccgcggat cacgcgacca tcgcggatca gcttatccat aattttggtt gctaactgga
    29461 aaggaatcgc aaagccgata ccttccggcg tttcgccatc gttactctta tcaaacgaca
    29521 gcgtgttaat gcccatcagt tctcccagcg agttcaccag cgcgccgcca gagttaccgt
    29581 ggttaatgga agcatcggtt tgtaggaagt tttgccgccc ggtcgggttc agaccgattc
    29641 gacccgtggc actaataatc ccttgggtaa tggtctgccc gaggttgtac gggttaccga
    29701 tcgccagtac tacgtcgcca atgtgcggta cgcgacgtgc attaattgga atggtaggta
    29761 aaccgccagt ggcattaatt ttaagtaccg ccagatcggt tagagagtca gatcccacca
    29821 gcaatgcttc aaatacacgt ccatcctgta aggcgacgat gatctgatcg gcgtcgttga
    29881 tgacgtgttt attggtgatg atataaccgc gttgatccat gattacaccg gatcccaggg
    29941 tgcggatctc aagctggttg tgagagttgg tgttcaaacc acggttgtaa acgttaacca
    30001 ccgctggcgc ggcgcggcga accgccagat tatagctggc aggcgtctca tcggtactgt
    30061 caaattgcgg agtggaaagc gggttaaggc tgcgcagcga aggcatggca accagcagaa
    30121 tagcgccgac aattaatcca atcgcaacgg aacgtaagag cttcacaaac atgatggagg
    30181 cgtcattaaa aaagggaacg gcagcagcat accacgagtt aaccggacat cacacgtcag
    30241 cctgatgccc ggtttacgac attaacgcat cagcagatag atgctttcat tgccgcgtac
    30301 aatttgcagg gcgatgatgg ccggttttgc cgccagcact ttacgcattt cagcaatcga
    30361 gttcacccga tcgcggttga cgccaatgat cacatcgtct ttttgcaagc cagcctgagc
    30421 agctgggctt cctttgacaa cttcatctat cttaatacct ttgccgccat cttttagctg
    30481 accatcgctc aacgttgcac cttccagcgc tggccgacga agaggtgctg gtatcaagcg
    30541 tcacttctac ttccagtggt ttgccgttac gcagcaggcc aagcttcact ttcgtgcccg
    30601 gctctgtagt cgcgatacga gagcgcaact cagcaaagct attcagcggt ttgccgttga
    30661 ggctggtaat aatatcgccc gctttgacgc ccgctttcgc tgagccagaa cttggcaaca
    30721 cttcgctgac aaacgcgcca cgctgcacgt caaggttgaa ggctttggcg atatcggcac
    30781 tcatctcggt gcctttgatg cctaacaaac cgcgtttgat ttcaccaaag tcgataagct
    30841 gctgcgccag tgttcgcgcc atattactgg ggatggcaaa tccaatcccg acgctcccgc
    30901 cgccaggcgc aaggattgca gtgttgatgc caattaactc accgttaagg tttaatagtg
    30961 caccgccgga gttaccgcgg ttaatggaag catctgtctg gataaagttt tccagacctt
    31021 caagattcaa cccgctgcgg cctaatgcgg aaacaatgcc agaggtggcg gtttgcccaa
    31081 ggccaaatgg gttaccgacc gctacggcaa aatcaccgac gcgcaattta tcggagtcgg
    31141 caatagcgat ttgcgttaat ttgctcgggt tttgaatttg taacagggcg atatcgctct
    31201 ggtcatcgct accaatcagt tttgcatcaa actcgcgccc atcattgagc tgaatactga
    31261 ttttctgtgc ctgattaatc acatggttgt tggtcagcac atagccttta ctggcgttga
    31321 tgatgacacc ggaacctaaa ccttcgaagg gttgtgcagg ttgatccggt aaatcatcac
    31381 caaaaaactt tttgaattct tccgggattt tctgtccctg actggccgtt ccttccaccc
    31441 gtacgctcac cactgccgga agcacttttt ccagcattgg agccagactg gggagagggg
    31501 cctgatcggc aacctggcct ggaatcgacg cgacggcctg aaatgacgcc gagagagtta
    31561 acccggcact taacgctaat gcactcaaca gctgggtttg ttttttcatt attcctgctc
    31621 tcgtacctga atgataagaa aagagattca aaacagtttg atgttattga attttcagac
    31681 ggctaacaat gagacaccag attaaatgat agtcggggag ggagagaaga ggagggcgca
    31741 atggctgcgc ccgaaaaata aattagtcgc gcttcgcgcc agtacgcagc aggccggatg
    31801 cgccttcaga atagtcgcga ggcacctgca ccggtgcctg atcgttgctg gcttcagact
    31861 ctgccagacg attacggaac gggtttgctt cagcagacag ttccggcagc aggctgctgg
    31921 agctttttgc catgtgctga tacagctggc gatagtcgtg cgccatggta tccagtaatt
    31981 ccgcgctgcg ggcaaagtgg ctaaccagct cttcgcgata ctcgtccagt tcagctttat
    32041 tcttttccag ttcgtactgc aacgcctgtt gctggcgtag tttacgatta ccaaaacgca
    32101 tggccacagc accaataatg atgccgacga ctaacccaat tagcgcatat tcccaggtca
    32161 tgaacatctc ccgttgtctt ttgtttccgt agggtgttgg cttcaggctc catgcctgcg
    32221 gctgattatg ccactataac cgttaattcc acagaagtgg aatcccgact gcatatcgcg
    32281 tagtgtagaa cggccttttt ttcgtcaacc gtgaacaacg gcgcaccgat tattcaagga
    32341 ataacaataa gatcatgcaa agcgttaccc caacatcgca atacctgaag gcgctcaatg
    32401 aaggcagcca tcaacccgac gacgttcaaa aagaggccgt cagccgcctg gaaattattt
    32461 atcaagaact catcaatagc aggccaccag cccccaggac gagtgggcta atggcgcggg
    32521 tcggtaagct gtggggtaaa cgcgaagaca caaagcatac gccagtgcgt ggcttatata
    32581 tgtggggcgg tgtaggacgc gggaaaacct ggctgatgga ccttttctat caaagcctgc
    32641 cgggagagcg gaaacagcgc ctgcactttc accgttttat gctgcgggtg cacgaagagc
    32701 taactgcctt acaggggcag accgatccgc tggaaattat tgccgatcgc tttaaagccg
    32761 aaactgacgt gctctgtttt gacgaatttt ttgtttctga tattaccgac gccatgctac
    32821 ttggcggtct gatgaaagcc ctgttcaccc gcggtattac cctggtagcg acgtcaaata
    32881 ttccgccgga cgaactttat cgaaatggcc tgcaacgtgc gcgttttctg cctgcaatcg
    32941 atgccattaa acagcattgt gatgtaatga acgtggacgc tggtgttgat tatcgactgc
    33001 gtacactcac tcaggcgcat ctgtggcttt cgccactcaa cgatgaaacc cgggcgcaga
    33061 tggataaact atggttggcg ctggcggggg tgaaacgaga aaattcaccg acgttagaaa
    33121 tcaaccatcg gccattggcg acaatgggcg tcgagaacca gacgctggcg gtttctttta
    33181 ctacgctgtg cgtcgacgcc cgcagtcagc atgactatat tgcgctctca cgcctgtttc
    33241 acacagtaat gttgtttgat gtgccagtca tgacgcggtt gatggagagc gaagcgcggc
    33301 gctttattgc gctggtggat gagttttacg agcgccatgt caaattagtg gtgagtgcag
    33361 aagtgccgct gtatgaaatt tatcagggtg agcggctgaa gtttgagttc cagcgttgcc
    33421 tgtcacgtct gcaagagatg caaagcgaag agtatctgaa gcgcgagcat ttggcgggtt
    33481 aaaacctgcc acaaatcaca aaaaggggtc gatctttgac cccgacttct ctataatcct
    33541 gcgaccccac gttacaagaa agtttttttc ccaaaacttt ttgtgtgctg gcataggcta
    33601 ttcgaagggg taggtttgcc ggactttgtc gtgtgaacct caacaattga agacgtttgg
    33661 gtgttcacca acgtgtaact atttattggg taagctttta atgaaaactt ttacagctaa
    33721 accagaaacc gtaaaacgcg actggtatgt tgttgacgcg accggtaaaa ctctgggccg
    33781 tctggctact gaactggctc gtcgcctgcg cggtaagcac aaagcggaat atactccgca
    33841 cgtagatacc ggtgattaca tcatcgttct gaacgctgac aaagttgctg taaccggcaa
    33901 caagcgtact gacaaagtgt actatcacca caccggccac atcggtggta tcaaacaagc
    33961 gacctttgaa gagatgattg ctcgccgtcc tgagcgtgtg attgaaatcg cggttaaagg
    34021 catgttgcca aaaggcccgc tgggtcgtgc tatgttccgt aaactgaaag tttacgcggg
    34081 taacgagcac aaccacgcgg cacagcaacc gcaagttctt gacatctaat cgggattata
    34141 ggcaatggct gaaaatcaat actacggcac tggtcgccgc aaaagttccg cagctcgcgt
    34201 tttcatcaaa ccgggcaacg gtaaaatcgt aatcaaccaa cgttctctgg aacagtactt
    34261 cggtcgtgaa actgcccgca tggtagttcg tcagccgctg gaactggtcg acatggttga
    34321 gaaactggac ctgtacatca ccgttaaagg tggtggtatc tctggtcagg ctggtgcgat
    34381 ccgtcacggt atcacccgcg ctctgatgga atacgacgag tccctgcgtt ctgaactgcg
    34441 taaagctggc ttcgttactc gtgacgctcg tcaggttgaa cgtaagaaag tcggtctgcg
    34501 taaagcacgt cgtcgtccgc agttctccaa acgttaattg gcttctgcta cggcagaaaa
    34561 caatttgcga aaaaacccgc ttcggcgggt ttttttatgg ctaaattctg aatcagcgta
    34621 aaaaatggga agttgctttt tgctgccgtc tgaaagacag gtaaaacaaa aaccacatcg
    34681 ccaataaggg tctaagtcaa ctatttcaga ctaaagcgca tctctttttc cccatttccg
    34741 gcatccactc accacaaagg tcgcaaaatc tggtaaacta tcatccaatt ttctgcccaa
    34801 atgtcgggta ttgctcattt tttgtttgat tttcgaacaa agagagtcgt tccttattgg
    34861 gtaacacaac ttctgactgg ccacctggtg gctggtagca gtaaaaattc tgactatacc
    34921 tggaggtttt catggctgtc gctgccaaca aacgttcggt aatgacgctg ttttccggtc
    34981 ctactgacat ctatagccat caggtccgca ttgtgctggc tgagaaaggt gtaagtttcg
    35041 agatcgaaca cgtggaaaag gacaatccgc ctcaggatct gattgacctc aacccgaatc
    35101 agagcgttcc gaccctggtg gatcgtgagc tgaccctgtg ggaatctcgc atcattatgg
    35161 aatatctgga tgagcgtttc ccgcatccgc cactgatgcc tgtttacccg gtagctcgcg
    35221 gtgaaagccg tctgtacatg catcgcatcg aaaaagactg gtacacgctg atgaacacta
    35281 tcatcaacgg ttcagcttct gaagcagatg ccgcacgtaa gcaactgcgc gaagaactgc
    35341 tggcgattgc gccggtcttc ggtcagaagc cgtacttcct gagcgatgag ttcagcctgg
    35401 tcgattgcta ccttgctccg ctgctgtggc gtctgccgca actgggcatc gagttcagcg
    35461 gcccgggtgc gaaagagctg aaaggctata tgacccgcgt ctttgagcgt gactctttcc
    35521 ttgcttcttt aactgaagca gaacgtgaaa tgcgtctggg ccggagttaa tctgtatgga
    35581 tttgtcacag ctaacaccac gtcgtcccta tctgctgcgt gcattctatg agtggttgct
    35641 ggataaccag ctcacgccgc acctggtggt ggatgtgacg ctccctggcg tgcaggttcc
    35701 tatggaatat gcgcgtgacg ggcaaatcgt actcaacatt gcgccgcgtg ctgtcggcaa
    35761 tctggaactg gcgaatgatg aggtgcgctt taacgcgcgc tttggtggca ttccgcgtca
    35821 ggtttctgtg ccgctggctg ccgtgctggc tatctacgcc cgtgaaaatg gcgcaggcac
    35881 gatgtttgag cctgaagctg cctacgatga agataccagc atcatgaatg atgaagaggc
    35941 atcggcagac aacgaaaccg ttatgtcggt tattgatggc gacaagccag atcacgatga
    36001 tgacactcat cctgacgatg aacctccgca gccaccacgc ggtggtcgac cggcattacg
    36061 cgttgtgaag taatacaaaa caggcccagg cggcctgttt tgtcttttta atgacttaat
    36121 aaaatagcgt gattgtggca atcatattca ccacgaaacc gactgccatg gggatcgctg
    36181 tccgcttcac caccgcaaaa ggcgaaacgc ctgcaattcc tgacgtaacg accaccaccg
    36241 cagtaatcgg cgaaaccgcg cgcgctagcg tcgtggcaaa atgcatcggc ataatcatta
    36301 caaccgctgg tacatgtagt ccggctgcga tattcggaat aagactggca aatgacataa
    36361 acggcgcatt gccagagccc atcacaatgg cacaaatggc aatgactagc gccataataa
    36421 tcatcacgcc aataccgccc agaccagaat gctccgcacc cctgataacc gcatcaacag
    36481 tgccaatcgt cgttaagcct ttcgcaaata tttcacccgc gaccaccagc gttaccacgt
    36541 tgacaaactg cgtacccatg ccgtcaaaaa acgcctgcac atcgtccatt gtttcgcgca
    36601 agttatgctt gcggaagaac tcaacaaaca tcgtcaccgt caaacttagt aacatcacca
    36661 ccaccagatg cagttctgac tgcatcagcc cgacgtgggc gaggaacagc gagccgagca
    36721 tcaggattaa cggcatcaca ggtaaaatgg cgtaatagag cggcgggaca ttatcgagag
    36781 ttttttgctc tgcctgttcg tgattgatat ttttatcttt tttgtcaaaa gcgcgttgca
    36841 cgaaaaagtg ggagatcgcc accgagataa tgacgcaaga ggcgaccgga agctggtagt
    36901 ggaagaagta tgtggcaatt ttcattcccg cgacctgggc tgcaaaaatg gagttcgttt
    36961 ccagaatccc ccactcaatg gacatcgtgg ttgcgataac cgccaccgca gagaggcgac
    37021 ttactcccag actcaccagc gtcggaaata aggtgaccat cagcaacata cccagaccgg
    37081 aggcgctggt aataaact
//
LOCUS       DAJTRW010000031        36544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRW010000031 DAJTRW010000000
VERSION     DAJTRW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(9..488)
                     /locus_tag="OO326_001707"
     CDS             complement(9..488)
                     /locus_tag="OO326_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5966119.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(672..2090)
                     /gene="gltD"
                     /locus_tag="OO326_001708"
     CDS             complement(672..2090)
                     /gene="gltD"
                     /locus_tag="OO326_001708"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5966120.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2103..6563)
                     /gene="gltB"
                     /locus_tag="OO326_001709"
     CDS             complement(2103..6563)
                     /gene="gltB"
                     /locus_tag="OO326_001709"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5966121.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7238..8167
                     /locus_tag="OO326_001710"
     CDS             7238..8167
                     /locus_tag="OO326_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5966122.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8263..10599
                     /gene="arcB"
                     /locus_tag="OO326_001711"
     CDS             8263..10599
                     /gene="arcB"
                     /locus_tag="OO326_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5966123.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10829..11482
                     /gene="elbB"
                     /locus_tag="OO326_001712"
     CDS             10829..11482
                     /gene="elbB"
                     /locus_tag="OO326_001712"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5966124.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11479..12206
                     /gene="mtgA"
                     /locus_tag="OO326_001713"
                     /pseudo
     CDS             11479..12206
                     /gene="mtgA"
                     /locus_tag="OO326_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12203..12835)
                     /gene="yrbL"
                     /locus_tag="OO326_001714"
     CDS             complement(12203..12835)
                     /gene="yrbL"
                     /locus_tag="OO326_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5966125.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13048..13320)
                     /gene="npr"
                     /locus_tag="OO326_001715"
     CDS             complement(13048..13320)
                     /gene="npr"
                     /locus_tag="OO326_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5966126.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13317..14171)
                     /gene="rapZ"
                     /locus_tag="OO326_001716"
     CDS             complement(13317..14171)
                     /gene="rapZ"
                     /locus_tag="OO326_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5966127.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14217..14708)
                     /gene="ptsN"
                     /locus_tag="OO326_001717"
     CDS             complement(14217..14708)
                     /gene="ptsN"
                     /locus_tag="OO326_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5966128.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14826..15113)
                     /gene="hpf"
                     /locus_tag="OO326_001718"
     CDS             complement(14826..15113)
                     /gene="hpf"
                     /locus_tag="OO326_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5966129.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15136..16569)
                     /gene="rpoN"
                     /locus_tag="OO326_001719"
     CDS             complement(15136..16569)
                     /gene="rpoN"
                     /locus_tag="OO326_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5966130.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16617..17342)
                     /gene="lptB"
                     /locus_tag="OO326_001720"
     CDS             complement(16617..17342)
                     /gene="lptB"
                     /locus_tag="OO326_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5966131.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17349..17906)
                     /gene="lptA"
                     /locus_tag="OO326_001721"
     CDS             complement(17349..17906)
                     /gene="lptA"
                     /locus_tag="OO326_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5966132.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17875..18450)
                     /gene="lptC"
                     /locus_tag="OO326_001722"
     CDS             complement(17875..18450)
                     /gene="lptC"
                     /locus_tag="OO326_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5966133.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18447..19013)
                     /gene="kdsC"
                     /locus_tag="OO326_001723"
     CDS             complement(18447..19013)
                     /gene="kdsC"
                     /locus_tag="OO326_001723"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5966134.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19034..20020)
                     /gene="kdsD"
                     /locus_tag="OO326_001724"
     CDS             complement(19034..20020)
                     /gene="kdsD"
                     /locus_tag="OO326_001724"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5966135.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20034..20999)
                     /locus_tag="OO326_001725"
     CDS             complement(20034..20999)
                     /locus_tag="OO326_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5966136.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21209..22018
                     /gene="mlaF"
                     /locus_tag="OO326_001726"
     CDS             21209..22018
                     /gene="mlaF"
                     /locus_tag="OO326_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5966137.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22026..22808
                     /gene="mlaE"
                     /locus_tag="OO326_001727"
     CDS             22026..22808
                     /gene="mlaE"
                     /locus_tag="OO326_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5966138.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22813..23364
                     /gene="mlaD"
                     /locus_tag="OO326_001728"
     CDS             22813..23364
                     /gene="mlaD"
                     /locus_tag="OO326_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5966139.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23383..24018
                     /gene="mlaC"
                     /locus_tag="OO326_001729"
     CDS             23383..24018
                     /gene="mlaC"
                     /locus_tag="OO326_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5966140.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24018..24311
                     /gene="mlaB"
                     /locus_tag="OO326_001730"
     CDS             24018..24311
                     /gene="mlaB"
                     /locus_tag="OO326_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5966141.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24471..24725
                     /gene="ibaG"
                     /locus_tag="OO326_001731"
     CDS             24471..24725
                     /gene="ibaG"
                     /locus_tag="OO326_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5966142.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24780..26039
                     /gene="murA"
                     /locus_tag="OO326_001732"
     CDS             24780..26039
                     /gene="murA"
                     /locus_tag="OO326_001732"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5966143.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26087..26365)
                     /gene="sfsB"
                     /locus_tag="OO326_001733"
     CDS             complement(26087..26365)
                     /gene="sfsB"
                     /locus_tag="OO326_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5966144.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26592..27563)
                     /gene="ispB"
                     /locus_tag="OO326_001734"
     CDS             complement(26592..27563)
                     /gene="ispB"
                     /locus_tag="OO326_001734"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5966145.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27821..28132
                     /gene="rplU"
                     /locus_tag="OO326_001735"
     CDS             27821..28132
                     /gene="rplU"
                     /locus_tag="OO326_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5966146.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28153..28410
                     /gene="rpmA"
                     /locus_tag="OO326_001736"
     CDS             28153..28410
                     /gene="rpmA"
                     /locus_tag="OO326_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5966147.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28537..29502
                     /locus_tag="OO326_001737"
     CDS             28537..29502
                     /locus_tag="OO326_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5966148.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29518..30690
                     /gene="cgtA"
                     /locus_tag="OO326_001738"
     CDS             29518..30690
                     /gene="cgtA"
                     /locus_tag="OO326_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5966149.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30876..32309)
                     /gene="dacB"
                     /locus_tag="OO326_001739"
     CDS             complement(30876..32309)
                     /gene="dacB"
                     /locus_tag="OO326_001739"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5966150.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32557..33033
                     /gene="greA"
                     /locus_tag="OO326_001740"
     CDS             32557..33033
                     /gene="greA"
                     /locus_tag="OO326_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5966151.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33189..33482)
                     /gene="yhbY"
                     /locus_tag="OO326_001741"
     CDS             complement(33189..33482)
                     /gene="yhbY"
                     /locus_tag="OO326_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5966152.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33608..34237
                     /gene="rlmE"
                     /locus_tag="OO326_001742"
     CDS             33608..34237
                     /gene="rlmE"
                     /locus_tag="OO326_001742"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5966153.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34337..36271
                     /gene="ftsH"
                     /locus_tag="OO326_001743"
     CDS             34337..36271
                     /gene="ftsH"
                     /locus_tag="OO326_001743"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5966154.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36361..>36544
                     /locus_tag="OO326_001744"
     CDS             36361..>36544
                     /locus_tag="OO326_001744"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5966155.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGES"
BASE COUNT         8573 a         9114 c         9881 g         8976 t
ORIGIN      
        1 tccttcgttt atttgcatga atatacaaac cgaaaatagg agatgcaata caaaagctgt
       61 ggaaatatca aacagcctct caatttggca gacagcgtct gccaaatctt tgccgatggg
      121 aattctgcag atggtgtctg cggaattccc aatggtgtta attcgccaga cactgtctgg
      181 cgaatttcaa catgttgaaa aaagaggtaa aaaagcctca tttggcgtaa gttttttgca
      241 gagaccccac gtttataccg tcgacttaaa tccttcgata gccgatcaat caactgtgca
      301 ccataagctg ctctggcctc accaccttgt tcaaattcga caatccgtcg gccaatttcc
      361 cagtacgtcg cggtcatcag cgcgttaacg ctacgtaccg tttccgttcg ggcgctatcg
      421 accagatgaa taatgccgtt gtggatttgc tggtagcctg ttgaggttct ctctgagagc
      481 gattccatta tctgtccctt tatatggcgc tgcgttgaat aatggaatca gctatacgcc
      541 gggcatctac cggtaacaat ccagatgagc gtattacatt ggttttcttc agattatgcg
      601 aggcgatttc cagaaagaaa ggagaatttc agcgccgact cttttctgag gaggcggcgc
      661 tgggacagag cttaaacttc cagccagttc ataatgccgt ctgccgcttt gcgaccttcg
      721 gcgatggcgg taacgaccag atcggaacca cggacgatat cgccgccagc aaagattttc
      781 gggttgctgg tctggaaagc gttgtcgttg ccttccggag cgatgatgcg gccttgtgaa
      841 tccagctcga cgctgtgttt tgccagccat tccatgttgt gtggacggaa accaaacgcc
      901 atgatcaccg catctgccgg aacgatatgt tcggaacctg caacgatctc cgcgcggcga
      961 cggcctttgg catccggttc gcccatttcg gtacgtacca tttttacgcc gctgactttg
     1021 ccgttaccgt tcacttcaat ccccagtggc tggacgttga atttgaactc tacgccttct
     1081 tcccgcgcgt ttttcacttc gcggcgggaa cccggcatgt tctcttcatc acgacgatag
     1141 gcacaggtaa cgtgctttgc cccctggcgt acagacgtgc gcacgcagtc catcgcagtg
     1201 tcgccaccgc caagtactac cacgcgtttg ccttccatgc tgacgaacgg ttcgtcgcgg
     1261 gtttcaccaa agcccattaa ctgtttggtg ttggcgataa ggaacggcag cgctgcgtac
     1321 acgccatcgg cgtcttcgtt ttccagcccg ccgcgcattg actgataagt cccaacgcca
     1381 aggaacacgg catcgtaatc actcagcaga tcgtccagct gtacgtcgcg gcccacttcg
     1441 gtattgagtt tgaattcaat acccatgccg gtgaagattt cacggcgacg cgtcattacc
     1501 tctttttcca gcttgaaggc cggaataccg aaggtcagca gcccgccaat ttctggatga
     1561 cggtcgaaga caacggcttt tacgccgtta cgcgtcagga catccgcaca cgccagacct
     1621 gccgggcctg caccgataat cgccactttt ttaccggttt gcttcacgcc ggacatatcc
     1681 ggacgccagc ccatttcgaa cgctttatca ttgatataac gctcaatgtt gccgatggtc
     1741 accgcaccga actcatcgtt cagggtgcag gaaccttcgc acagacggtc ttgcgggcag
     1801 acacgtccgc atacttccgg cagtgtgttg gtctgatgcg acagttctgc cgcttcaaaa
     1861 atacgcccct cgttggcgag cttcagccag tttgggatgt agttgtgtac cgggcatttc
     1921 cactcgcagt atgggttgcc gcacgacaga cagcggtcag cctgcgcttt ggcctggcct
     1981 tcggaaaacg gctcgtaaat ttcaacaaac tcaattttac ggatcttcag cggtttcttt
     2041 ggcggatcaa cgcgctgcag gtcgataaat tgataaacat tctgactcat tgttgctacc
     2101 ccttactgcg cctgcacgcg caactctgct gcgctacgac tacggtgacc cagcagtgct
     2161 tttacatcac tggacttcgg tttaaccagc gcgaatttcg tggcgaaggt tgaccagttc
     2221 gccagaatct cttcaccgcg ctgagaaccg gtatgctgaa catgctcggt gataagaccg
     2281 cgcagatgct cttcatggat cgccagatcg tcgacgctta agacttcgac cagttccggg
     2341 ttaacgcgtt tgcggaaatc accgctttca tcgagaacgt aagcgaagcc gccggtcatg
     2401 cccgcgccga agttaacgcc ggttttaccc agaatacaga caataccgcc cgtcatatat
     2461 tcacagccgt tgtcgccaat gccttctacc acggtgatag caccggagtt ccgcacgccg
     2521 aaacgttcac ccgcgcggcc tgcggcatac agacgaccac cggtcgcgcc atacaggcag
     2581 gtgttgccga taatgcttgc ttcatggctg cggaaggcgg aaccaaccgg aggacgaatg
     2641 gcgattaagc cgcccgccat gcctttaccg acatagtcgt tggcatcccc ggtcaggtac
     2701 agttccacgc cgcccgcgtt ccacacaccg aaactctggc ctgcggtgcc gttgaagtac
     2761 gctttgatag gatcggctgc cagcccctga tcgccgtgcg tctgggcgat atagcctgaa
     2821 agcgacgcgc cgacagaacg gtcggtgttg cggatatcga accagaaggt tttgctctgg
     2881 cgttcatcga caaacggttt cgcctgttgc agcaactgcg cgttcagtag gccgttatca
     2941 aacggcgggt tgttttcagt gcagtagagt gacttacctg catgtggctc ggcagtctcc
     3001 agcagcttcg acagcgccag tttctgctgt ttggcggtga aaccgtccag ctctttcagc
     3061 aggtcggtgc gaccaatcag atccaccaga cgcgttactc ccagctgtgc catcagctcg
     3121 cgggtttcac gggcgataaa ctcaaagtaa ttagtcacct tgaacggcag gccgtgatag
     3181 tgattcttac gcagtttgtc atcctgagtt gctacacccg tggcgcagtt gttcaggtgg
     3241 caaatacgca gatatttaca gccgagcgcc accattgggc cagtgccgaa gccgaagctt
     3301 tctgcgccga gaatcgccgc tttgatgata tcgacacccg ttttcaggcc gccatcaacc
     3361 tgcaaacgaa ttttatgacg caagccgttg gcaaccagcg cctgttgggt ttccaccaga
     3421 ccgagttccc acgggcagcc tgcatatttc actgacgaca gcgggcttgc gccggtgccg
     3481 ccgtcatagc ccgcgatggt gatcaagtcc gcataagctt tcgccacgcc agtcgcgatg
     3541 gtgccaacgc ctggttcgga caccagctta acggagatca tcgctttcgg gttaacctgc
     3601 ttgaggtcga aaataagctg cgctaagtct tcgatagagt agatatcgtg gtgcggcggc
     3661 ggggagatca gcgtcactcc gggcaccgaa tagcgcagtt tggcgatgta aggagtgact
     3721 ttgtcgcccg gcaactgacc accttcgccc ggcttcgcgc cctgggcgac tttaatctga
     3781 atgacgtcgg cattgaccag atacgccgga gtaacgccga agcgaccaga agccacctgc
     3841 ttgatgcgcg acactttgtt agtgccatag cgcgccgggt cttcgccgcc ttcaccggag
     3901 ttcgagttac cgccgatgct attcatcgct tccgccagcg cctcgtgggc ttccgggctt
     3961 aacgcgccga tagacatcgc ggcggtatca aagcgtttaa acagttcgct tgccggttca
     4021 acatcagcaa tgttgaccgc gttttcaccc ggcgtaattg ccagcagatc gcgcagcgtg
     4081 gttgccggac gctcattaac cagcttcgcg tattcctgat agtcgctgta ctcgccgctt
     4141 tgtaccgcct gttgcagcgt gcgcaccacg tccgggttgt aggcgtggta ttcgccgccg
     4201 tggacgtatt tcagcaggcc gccctggcta atcggcttgc gcgccagcca ggcacgtttc
     4261 gacagattca gcagatcctg ctggaagtct tcaaagcttg ccccaccaat gcggctgacc
     4321 gcgccctgga agcacaggcc cactacatca tcgtgtagac cgaccgcttc aaacagtttc
     4381 gagcagcggt aagaggcgat ggtggagatg cccattttgg acatgatttt gtacaagcct
     4441 ttgttgatgc cgttacggta gttgagcatc acggtacgat aatctttggc aatcgcgtgg
     4501 gtgtctacca ggcggcccag cgtttcatag gcaaggtacg gataaatagc cgtcgcgccg
     4561 aaacccagca acacggcgaa gtggtgcgga tcgcgggcgc tggcggtttc gacgatgatg
     4621 ttggcatcgc aacgcaggct ttgatcgacc agacgggtct ggatcgcgcc aaccgccatc
     4681 ggagccggaa ccggcaggcg atctttagcg atattccggt cggagagcac cagcaacacg
     4741 gtgccgctac gtaccatttt ttcggctttg tcgcatagct ctttgactgt cgcttcgagc
     4801 gtggttttag tgacgtcaaa ggtgatatcc agcgtatctg cgcggtagtg ctcctctttc
     4861 atcgtcgtga gctgtttgaa atcggagtag agcagaatcg gcgatttaaa gcttaaacgg
     4921 tgcgcctggc cctctgcttc gcaaaagacg ttcatttcac gaccgatact ggtggcgagc
     4981 gacataacat gcgcttcacg cagcgggtcg attggcgggt tagtcacctg ggcaaactgc
     5041 tggcggaagt agtcgtaaat aatgcgcggc tgactggaga gcacggcgaa tggggtatca
     5101 tcgcccatcg aaccgaccgc ttcctgaccg ttttcgccca gtacgcgaat tacggagtcc
     5161 agctcttccg cgctgtagtt aaactgtttc tggtagctgg caagcgtgtc gtcgtccagt
     5221 tcgcggctac ccacttcttc atcgggcaga tcttcaaacg gtaccagtcg gcggacgttt
     5281 ttctccatcc actctttata agggtggcgg cttttcagat cgtcatcggt ttctgccgag
     5341 tgcagaatac gcccactgcg agtgtcgatc accatcagtt cgcctggccc gacgcggcct
     5401 ttttcgacca cttcgtcagg ctggtaatcc cagataccga cttcagaggc gcaggtgatg
     5461 agcttgtctt tggtaatgac gtagcgtgcc ggacgcagac cgttacggtc gaggttacag
     5521 gcagcgaagc gaccatcgga catcacgatg cccgccggac catcccacgg ctccatatgc
     5581 atggagttaa agtcaaagaa ggcacgcagt tccgggtcca tatccgggtt gttctgccag
     5641 gcgggtggca ctaacaggcg catggcgcgg atgatatcca tcccgcctgc cagcagcagt
     5701 tccagcatgt tatccatgga actggagtca gaaccggttt cgttgacgaa tggcgcggcg
     5761 tcgtgcaggt cagggatcag cggcgtctgg aatttatagg tacgcgcacg cgcccattgg
     5821 cggttaccgg tgatggtgtt gatttcaccg ttatgcgcca gatagcggaa cggttgcgcc
     5881 agcggccagc gcggtacggt gttagtggag aagcgctggt ggaacaggca aatggccgat
     5941 tccagacgca ggtccgcaag atccagataa aagcgcggca gatccgccgg catacacaga
     6001 cctttataga tgttcaccag attcgacagg ctacagacgt agaagtcttt gtcggcttcg
     6061 agacgctttt caatgcggcg gcgggcgata aacagacggc gctccatatc gcgtggacgc
     6121 cagcctgccg gggcgttcac aaaaatttgc tcaatgcgtg gcagagagga gagggcgatt
     6181 tcacccagca cgccttcgtt agtggggaca tcacgccagc ccacaatcga caaggtttcg
     6241 cgttgcagtt cttcttcaac gatgcggcgt gcggcagcgg cgagttcagg atctttattc
     6301 aggaagagca tcccgacagc gtagtttttt gctaaacgcc agccgcgctc ctgcgcaacg
     6361 atgcgaaaaa agcgatccgg tttttgtaac agcaagccgc aaccgtcgcc ggttttacca
     6421 tcggcgagaa tcgcgccacg gtgctgcatg cgggccagtg cgtgtattgc agtacgcact
     6481 accttgtggc taggttcgcc ttctatgtgg gcgatcaggc cgaaaccaca gttatccctc
     6541 tcaagggatt tatcgtacaa catatcgtga acctccccca ggctctgcgg gaaccccact
     6601 tccgaaccgc tgcgcacggg cacagaaaga gcgtggcgac ggggtttgcg tgtcatacgc
     6661 gcgcctcgca ttcgccccct tgttgtcctt ttcgcatcgg ttaatacggt gaagagggga
     6721 tctcaattac tgcataaata tgatgagcag gctgctcatc cagaaagctt ccagcggatt
     6781 tccaacttat cgggaaagct gatacaggtc aaatggcaag cttattggta cagaaatgtg
     6841 ctttagcgca aaattcttta ttaaatagct gaaattaaaa tagattttat ctttaaggct
     6901 aaaaggagag tcgttagaga aacagtcaat tcctgactgt cataaaggcg tgttaaccct
     6961 tatcctgcct tgaactgacg agaaaaccag cattttatac tgccttaatt ggtaatgacg
     7021 tttcatgcta ctgtttttgc ctaaaatcca taaggagata aaaaaatctg acacaagata
     7081 agcaaaagtg atttatgtga ttgtgcatga aatttcggta attttgattg attatgcagt
     7141 tgataaaagc aggccgggac gattgatcca ggtcatcgcc gaattgagct aaaaatggca
     7201 ggctttggcc tcttttcaag gcgcggtcta tcagattatg cagttacaga aattagtcaa
     7261 tatgtttggt ggtgatctca cccgtcgtta tgggcaaaag gtgcataagc tgacgctgca
     7321 cggcggtttt agctgcccta accgtgacgg taccatcggg cgtggtggct gcacattctg
     7381 taatgttgcc tcgtttgccg atgaagcgca gcagcatcgt tccattgccg agcaactggc
     7441 gcaccaggcg aatttagtta accgcgctaa acgctatctg gcctactttc aggcgtatac
     7501 cagcaccttt gcggaagttc aggtgctgcg ttctatgtat cagcaggcgg tgagccaggc
     7561 caatattgtc ggtttgtgtg ttggtacccg cccggactgc gtgccggatg cggtgctgga
     7621 tctgctttgc gaatataagg accagggtta cgaagtgtgg ctggagctgg ggctacaaac
     7681 cgcccacgac aaaacactgc atcgcatcaa ccgcggccat gattttgcct gttatcagcg
     7741 tacaacccag ctggcacgtc agcgcgggct gaaggtgtgt tcccacttaa ttgtcggcct
     7801 gcctggcgaa gggcaggctg aatgtttgca aacgctggag cgagtggttg aaaccagcgt
     7861 ggacggcata aagctgcatc cgctgcatat tgtgaaaggc agcattatgg cgaaagcctg
     7921 ggaagcgggg cgtttgaacg gtattgaact ggaggattat acgctcactg ccggagaaat
     7981 gattcgccat acgccaccgg aagtgatcta ccaccgcatt tccgccagcg cccgtcgccc
     8041 gacgctgctt gctccgctgt ggtgcgaaaa tcgatggacg ggaatggtcg agctggatcg
     8101 ctatctgaat gaacatggcg tacagggatc ggcgctggga cgtccgtggc tacctccaac
     8161 ggagtaggtc gttgagggga attccgcatt tctcacacaa tttataacgt aactgtcaga
     8221 attgggtatt attggggcag gttgtcgtga aggaattccc taatgaagca aattcgtctg
     8281 ctggcgcagt attatgttga cctgatgatg aagttaggtc tggtgcgctt ctcaatgttg
     8341 ctggcgctgg ccctcgtcgt tcttgccatt gtggtacaaa tggcggtaac catggtgctg
     8401 catggtcagg tcgaaagcat tgatgttatt cgttctatct tctttggttt gctgattacg
     8461 ccgtgggcgg tctactttct atcggtggtc gtcgagcaac tggaggagtc acgacaacgt
     8521 ctgtcacggc tggtgcaaaa actggaggag atgcgcgagc gcgatttgag cctcaacgtt
     8581 cagttaaaag ataatattgc ccagctaaat caggaaattg ccgttcgtga aaaagcggaa
     8641 gcagaactgc aggaaacctt cggccaactg aaaattgaaa tcaaagagcg cgaagagaca
     8701 caaattcagc tcgagcagca atcctcattc ttacgttcct tccttgatgc ttcacccgac
     8761 ttggtttttt atcgtaacga agataaagag ttttccggct gtaaccgcgc gatggagctg
     8821 ctgaccggaa aaagcgaaaa acaactggtt cacctgaaac ctgctgatgt ttactcaccg
     8881 gaagccgccg caaaagtcat tgaaaccgat gaaaaagtgt tccgtcataa tgtgtcactg
     8941 acctatgaac agtggctgga ttacccggac gggcgcaaag cctgctttga aatccgtaaa
     9001 gtgccgtact acgaccgcgt gggtaaacgt cacggtttga tgggctttgg tcgcgacatt
     9061 accgagcgta agcggtatca ggatgcgctt gaacgcgcca gccgcgacaa aacgacgttt
     9121 atttccacca tcagtcacga attgcgtaca ccgctgaacg gtatcgtcgg cctgagccgc
     9181 attctgctgg ataccgaact caccgccgag caggaaaaat atctcaaaac tatccatgtt
     9241 tcggccgtca cgctggggaa tatctttaac gatattatcg acatggataa gatggaacgg
     9301 cgcaaggtcc agcttgataa tcagccggtt gatttcacca gcttccttgc cgatctggaa
     9361 aatctctccg cattgcaggc gcaacaaaaa ggattgcgct ttaacctgga gcctacgctg
     9421 ccattaccgc atcaggtcat taccgacggg acgcgtttac ggcagatcct gtggaacctc
     9481 atcagtaacg ccgtcaaatt cacccagcaa ggccaggtta ccgtgcgcgt gcgctacgat
     9541 gaaggcgata tgctgcattt tgaagtggaa gattctggta tcggcattcc gcaggatgag
     9601 ctggataaaa tcttcgccat gtattaccag gtgaaagaca gtcatggcgg taaacctgcc
     9661 accggcaccg gtattggtct ggccgtttct cgtcgtctgg cgaaaaatat gggcggcgat
     9721 attacggtta ccagtgaaca gggcaaaggt tcaaccttta cgttgacgat ccacgcaccg
     9781 tcggtggcag aagaggtcga tgatgcgttt gatgaagacg atatgccttt accggcgctg
     9841 aatgtgctgc tggtggaaga cattgaactg aacgtgattg tcgcgcgttc tgtgctggaa
     9901 aaattaggta acagcgttga tgtcgccatg accggcaagg cggcgctgga gatgtttaaa
     9961 ccgggcgaat acgacctggt gttgctggat attcagttgc cagatatgac cgggctggat
    10021 atctctcgtg aactgacgaa gcgttatccg cgcgaggatt taccgccgct ggtggcctta
    10081 accgctaacg tgctgaaaga caaacaagag tacctcaatg ctggaatgga tgatgtgctg
    10141 agtaagccgc tttcggttcc ggcgctaacc gcgatgatca agaaattctg ggatacccag
    10201 gatgatgagg agagtacggt gacgacagaa gagaacagta aatcagaagc attgctcgat
    10261 attcccatgc tggaacagta tctcgaactt gtaggaccga agctgatcac cgacgggtta
    10321 gcggtgtttg agaagatgat gccgggctat gtcagcgtgc tggagtcgaa tctgacggcg
    10381 caggataaaa aaggcattgt tgaggaagga cataaaatta aaggtgcggc ggggtcagtg
    10441 gggttacgcc atctgcaaca gctgggtcag caaattcagt ctcctgacct tcccgcctgg
    10501 gaagataacg tcggtgaatg gattgaagag atgaaagaag agtggcgtca cgacgtagaa
    10561 gtgctgaaag cgtgggtggc aaaagccact aaaaaatgac cccggctaga ccggggtgcg
    10621 cgaatactgc gccaacacca gggaaatctt ggctgcgccg taaattatta tgatgagtta
    10681 caagggcaca gcactgtttt tcaggccgca cgcagttaag atagcaaaac ttaaatgatt
    10741 tgttacatga atcagttaaa tgtgtgatgc gtaccatttt tattaaaaaa attaatgtgt
    10801 ttcagtcaat tgcgaaagga tgatcacaat gaagaaaatt ggcgtaattc tgagcggatg
    10861 cggcgtctat gacggttctg aaattcatga agcggtgttg acgttgttag ctatttcacg
    10921 cagcggtgcg caggcggtct gctttgcacc ggataagcag caggttgatg ttatcaacca
    10981 tttaactggc gaagcgatga cggaaacgcg caatgtgctg attgaagcgg cacgaataac
    11041 gcgcggtgaa atccgtcctc tggcccaggc cgatgccgct gaactggatg cgttgattgt
    11101 gccggggggg tttggcgcgg cgaagaattt aagcaatttt gccagtcttg gtagcgaatg
    11161 caccgttgac cgtgaattaa aggcgctggc acaagcgatg catcaggccg gaaaaccgct
    11221 tggttttatg tgtattgccc cggcgatgct gccgaaaatt ttcgatttcc cgctgcgttt
    11281 gaccatcggt actgatatcg ataccgcaga agtgctggaa gagatgggcg cggagcatgt
    11341 gccgtgtcct gtcgatgata tcgtggttga tgaagacaat aagattgtca ccaccccagc
    11401 atatatgctg gcgcagaaca ttgcagaagc ggcgagcggc attgataagc tggtttcccg
    11461 cgtgctggtt ctggctgaat gagtaaaagc cgcttaacgg tgtttagttt cgttcgccgt
    11521 tttcttttgc ggttaatggt tgtcctcgcc gttttctggg gcgggggcat cgcgttgttt
    11581 agcgttgcgc ctgttccctt ctcagcggta atggtcgagc gacaggtcag cgcctggctg
    11641 catggcaatt ttcgttacgt ggcacattct gactgggtca gtatggatca aatctcgccg
    11701 tggatgggac tggcggtgat tgccgcagaa gatcagaaat ttcctgagca ctggggcttt
    11761 gatgtcgctt ccattgagaa agccctggcg cacaacgagc gcaatgaaaa ccgtattcgc
    11821 ggtgcttcaa cgatttctca gcagacggcc aaaatctctt tttatgggat gggcgtagct
    11881 gggtacgaaa agggctggaa gctggattaa cgctggggat agaaacagtc tggagcaaaa
    11941 agcgtatcct gacggtttac ctgaatatcg ccgaatttgg cgacggtgtg tttggcgtcg
    12001 aagctgcggc acaacgttat ttccacaaac ccgcgagcaa acttacccgg tcggaagctg
    12061 cattactggc agctgtatta cctaatccac ttcgtttcaa agtctcctcg ccatcgggct
    12121 acgtgcgtag ccgtcaggcg tggattttac ggcagatgta tcagttaggc ggtgagccgt
    12181 ttatgcaaca acaccagctg gattaagctt cgcgcgaagt gagggccagc gttttgctct
    12241 cacgaggttg cagatctttc tgcagggcaa tagccagcgc cggttgggcg ataaatcgtt
    12301 tccataaccg ctcttgctta cgcagacaac accacttcga ccaggtcgcc agtggaatca
    12361 atgtgctttc accaatgttg tcgcagacga ccgggataac ctctgactca ctgatgcggt
    12421 gacagaggat attctgcggc ttcagcgaca tcgtcacgat acggttatcc tgcaaataac
    12481 gcttcagctg tttcagtagc tggcgcagtt gtgcaatgtc ttcttcgtaa cggcattgtt
    12541 cagcaaattc ggtcagcgta atggaaggct tgccgtcaaa atcggcaatc acatcgtaga
    12601 cataaccggt tccgcaatcc gtctcaacgg taccgtgata gcgcggtatt ccactccagt
    12661 cttttaaccg gcgaccaaga tgcgcgtagt actttaactc acggcggatc tctttatcgc
    12721 caccatcgcc acgatggtag acaatcttga tacagcgttg ggcatcttcc ggatgcgcat
    12781 aacatttccg atgtcttccg gtgcccaggg gactttgttc agataagcga atcatgccat
    12841 ctcctggaaa tattttctga cgatctccag tatggtcgat taacttaaac aaaacctaaa
    12901 caatgcctct taaggttttc ttaaggttct tctgaaagtg aaaagcgcac aaaatgtaca
    12961 aatgacgttg attgagaacg atttcagtac gtgattttct tattgtcggg ggagcttgaa
    13021 gggagttgta tgtcaaagtg atgaagatta atcttcatca aaaccagaat taaagagggc
    13081 gataacggcg gccagtgctt cctcttcctg tggaccggtc gcttcaactt caatctgccg
    13141 tcctttggca gaatccaaca tcagcagcgc aataacgctg ttggcttcag cctcggtgcc
    13201 ttcgtcatta cgtaacagca cttcagcgtc aaaaccctgc attaattcaa acagcttcat
    13261 tgcaggccgg gcatgcatgc ccagcttgtt tgtgatttca acagtttgct tgacggtcat
    13321 ggtttacgtt tttccagcgt acgatggcgt gactggacgt ttttaccgcg cgagcggaag
    13381 tagtctgcca gttgctctgc aatatacacc gaacggtgct tcccgccggt acaaccaatg
    13441 gcgaccgtca ggtagctacg gttgttggtt tccagcatag gtaaccatag ctcaagatag
    13501 cttcgcgtct ggtagataaa attgtgtact tctgtgtggc ggtcgaggaa cgcggcgaca
    13561 ggtttatcaa gacctgtcat tggacgcagt ttcggatccc agtgcgggtt cggcaagaag
    13621 cgcacgtcaa agacgtaatc tgcatcgata gggataccgt gtttgaagcc gaaagactca
    13681 aagaccatgg tcagttcgcg ttcacgttta cccagcagac gggtacgcag catttcagcc
    13741 agctcgtgaa cggacatttc tgaggtatcg acaatcagat ccgctcgcga acgcagaggc
    13801 tccagcaaat cgctttcttt gtcgatagca ctttccagcg acaggttttt gctggaaagc
    13861 ggatgcagtc ggcgcgtgtc actgtaacga cgaattaagg tattacggtc ggcatccagg
    13921 aacagtagtt gcggtgagaa agcgtcaggc aggttactca tcgcctgttc gaatatttct
    13981 ggtgactccg gcatattacg aacatcaatg ctgacggcgg cagaaatctc gcgatcggcc
    14041 agagttcgag ccagatcggg taacaacact acgggaaggt tatccacgca gtaaaaaccc
    14101 atatcttcca gcgcacgcag ggcgacagat ttacctgaac ctgaacgtcc gctgacgatc
    14161 atcagtacca tgtactgttt ctcctcacaa cgtctaaaag agacattacc gaataactac
    14221 gcttcatccg gagtaccttc ggtatccgta atgatttgat acagctcttc atcgctctgg
    14281 gctgcgcgta aacggcggca gatggttttg tccgccagac gtttcgccac cagcgacaga
    14341 gtatgcaggt gcgttttagt ttggtctgcc ggcaccagca gggcaaaaag taagtccacc
    14401 ggttggttgt cgatggcatc gaaagctata ggcgtttcga gctgaacgaa aacgccaacg
    14461 gcacgcagag tatcttcttc aagtttgcca tgcggaatgg caataccatt gccgataccg
    14521 gtactgccca ttttttcccg cgtcaggata gcttcaaaaa ccacctgagg cggcaggcta
    14581 agttgtttcg ccgccagctc gctgatgatt tccagggcgc gttttttgct ctgacagtgg
    14641 acgcggcttc gcgtacattc cctgttaaga acactgctaa gctgtagagt tgtatcatta
    14701 tttgtcataa tttcacctaa gaacctgccc actcaaacag tggaacaggc tcggagcatt
    14761 aaccgtacaa atggcccgtt gtgccgcaca acaggccgcc atgcacatgc taattgcccg
    14821 gacaattagt gttgtttcag tttatcttta tgtttggtca actggcgtgc cagcttatca
    14881 attaagccat caatggcagc gtacatgtcc tgaccttccg cgctggcatg aatttcacca
    14941 ccgtttacat gcagtgttgc atctgaggtg tgggtgactt tctccacttt cagaacaaca
    15001 tagacctggt tgattcggtc aaaatattgc tcaagtttgg caaatttagc tgtaacaaat
    15061 tcgcgcagtg cctcggtgat ctcgacgtta tttccggtaa tgttgagctg catagtgtct
    15121 tccttatcgg ttgggtcaaa cgagctgttt acgctggttt gacggcggaa tggataaaga
    15181 ctctcggtac ttcgcaacag tgcggcgtgc caccatgata ccttgttccg acagcaaaga
    15241 ggttaacttg ctgtcgctca acggtttcgc tgggttttcc gccgcgatta atttcttcac
    15301 cagtgcacga atcgccgtgg aggaagcttc gccgccgccc tcggtattga cgtgactgga
    15361 aaagaaatac ttcagttcaa aaatgcctcg tggactatgc aggtattttt gcgtggtcac
    15421 gcgagatatc gtcgattcat gcatttcgac agcctgggcg atatcggcca gtaccatcgg
    15481 tttcatatat tcttcacctt gctcaaagaa ggcttgctgc tgttcaacga tacagcgact
    15541 cacgcgcagt agcgtatcgt tacggctttc cagactcttg atcaaccatt tggcatcctg
    15601 cagattgctg cggataaact ggctgtcacc atcgttgcgc gcgttattgc acatcgaggc
    15661 gtagtgctgg ttgatttgca gacgcggaat gctgtcactg ttgagttcta ccgtccagtg
    15721 accgttatgc ttacgcacca gcacatctgg aatgacatac tcaggttcgc cagtctggat
    15781 cgactgcccg gggcgcggat cgagcgactg gatcagattg acggcttctt tcagcacatc
    15841 ttctttcaga cgcgtgacgc gcattaaagt gcggaagtcg tgattggcta acagatcgag
    15901 atgatcgcta atgatcaatc tggcctcttc cagccacggc gtggtcttat cgaattggga
    15961 gagttggatt agcaggcagt cacgcagatc ttttgccgct acaccgaccg ggtcaaaccg
    16021 ttggatccgc ttaaggacgg cttcaacctc gtcgatgtcg atctcttcat cgcccatact
    16081 ttcgagaata tcttccagcg ggacagtcag ataaccggtt tcatcaacgg catcgacgat
    16141 agaggtagca atagcgcggt cagtgtcgga aaacggtgtc agctcgacct gccacatcag
    16201 gtaatcctgc aaggtctgcg tcgtttcgcc ctggtagacc ggcagctcgt cgtcaatgta
    16261 gtcaccgctg gtgccggatg gtgtaccagc ggtgtaaatg gtgtcccaac tggcatcgag
    16321 cggcagctct tccggcatct ctttttgttc gagcgcgtcg gcggtgtcca gcgtttcact
    16381 gtcttgcgtt tcgcgggtgt cgatttcttc atgagtgtcg atttgctcaa gcagcggatt
    16441 actctccagc gcctgctgta gctcctgctg aagttccagc gtcgacaact gcaacagacg
    16501 aattgcctgt tggagctgtg gcgtcatcgc cagttgttgg ctaagcctga gttgcaaacc
    16561 ttgcttcatg ttcagaatcg tactctcctg ctaaaacgtc gcaaacttct accctatcag
    16621 agtctgaagt cttccccaag gtatacacgc ttaacgtgtt cgtcttgtaa gatttctgta
    16681 ggcgtgccgt gggcgatcaa atgcccctga ctgacgatat aagcgcgttc acaaaccgcc
    16741 agtgtttcac gcacgttgtg gtcagtgatc agcacgccca ggccgctgtc gcgcaggtgt
    16801 tcaatgatgc gtttaatgtc gataaccgag atcgggtcaa ccccggcaaa cggttcgtcg
    16861 agcagaataa atttcggatt cgcagccagt gcgcgggcaa tttctacacg gcgacgttca
    16921 cccccggaga gtgactgccc catgctgtca cgcaggtgct caatgtgaaa ctcttccatc
    16981 agctcgttcg cgcggtcttc acgttgttca gcagacaagt cgtcacgaat ttgcagtacc
    17041 gccatcaggt tatcgtaaac gctgaggcga cggaaaatgg aggcttcctg tggcagatag
    17101 ccgataccgc ggcgcgcgcg tgcatgcaga ggcagcagac tgatatcgtc atcatcaata
    17161 atgatgttgc ccgcatcgcg cggcacaatg cctacaacca tgtagaaagt ggtggtctta
    17221 ccggcaccgt ttggccccag cagaccgaca atttccccgg agttgacggt caggctgacg
    17281 tcttctacca cgcgacggcc tttataggct tttgcaaggt tctttgcagt taatgttgcc
    17341 ataacgaatt aattaccctt cttctgtgcc ggggtctggc ctttgttgtt tttgtcctgc
    17401 agctgcgacg gcaccagaac ggtggttacg cgcttgcctt tgtcgctgaa agcctgcatt
    17461 ttctgctctt tcaccaggta agtgatctta tcgcccttaa tgttgctatc gacctgctgc
    17521 agataagcat tacccgtcag aacgacaaaa tcttttgcca gttcgtagtg catctgggaa
    17581 gcgtgacctt caacgggttt accgttgtcc tgcatctggt agaacgttgc cggtttaccg
    17641 tatccgtcaa tcacttcttt accttgttcg ccgcccggac gggtaacgac cactttgtcg
    17701 gcattaattt tgatggtgcc ctgggtgacg atgacattac cggtaaaggt aaccacgttg
    17761 ccttgcatat caagagattg ctggtccgat tcaatgtgga tcggctgatc agtgtctccg
    17821 gttacggcaa atgccggaat gctggcggcc agaagtgagc tggcaagcac aagattaagg
    17881 ctgagtttgt ttgttttgaa tttcatagga tgttctaacc ttttcaatca gctcggcgtt
    17941 cttgctgcgt aagttgccgc gcattttcag accgctggag ttaaatgttg ttccgtataa
    18001 cgtgacgagg tcttcagagg taacatcctg cgtcaccaga ttgatctgcg cgttatccgt
    18061 cgtgattctg cgaagttgag agtctggcac aagtgcgttg acttcaacgt gtccatataa
    18121 atagagcatc cggtcattgg tcagcttggc tttatctgct tttacggacc atgtcgggat
    18181 tttatcctta tcaaacgtgg taagtaccgg ctgcgtaaac cacgaaacgg cctgatcgga
    18241 ataatattca acgtgttgag caatcaatcg atagcttagt gccccttctg ggttatagac
    18301 gagcgtgtcc gtatgctcgc ttttataggt gggatcattg ttgttgacga ccacctgggc
    18361 ggtatcgtct ttttcggcca tattaatgcc gatcatcacc agaaccgcca gtgatagcac
    18421 aatgataacc caacgtctgg ctttactcat atcgattgcc ctttggcttc atccagtttg
    18481 ccctgcgcca ggagtaataa gtcgcaaact tcgcgcactg cgccacgacc gccagcaatg
    18541 cgcgtcacgt aatcggcgcg cgggatcaac agtggatgcg catcggccac ggcgacgctt
    18601 aaacccactt tttccattac cggccagtcg atgagatcat cgccaacata agccacattt
    18661 tccggggcaa tcgccaattt ttccagcaaa tcgctaaagg cgatcagttt gtttgactgc
    18721 ccctgataca agtgagtgat ccccaatgtg gcacaacgat cttctaccag tttagccttt
    18781 cgcccggtaa tgatagcgac ttcaatatca gaggtgagcg cacaacgaat gccataaccg
    18841 tcacgaacat tgaacgcttt cagctcttcg ccgttattgc ccatataaat caggccatct
    18901 gacagtacgc catcaacgtc gaggatcagc agacgaatgt tctctgcttt tgccataacg
    18961 tcggcgctga cagggccgta acaggtcgca agcgacgcac ctgctttgct cattgttgtt
    19021 tatccttgaa tctttacact acgcctgcac gcagtaaatc atgcatatgt aacacaccga
    19081 gtaaatggtc gccatcggca accatcacgg aggtgatatg gcgggactgc attaagttca
    19141 gtgcctcaac ggccagaatg ccagggcgca cacgtattcc ccccggcgtc atcacatcgg
    19201 caatacttaa ctgacgaaca tccacgccca tatcgaagac acggcgtaaa tcaccgtcgg
    19261 taaagatgcc ttcaatcatc atattgtcat cgcaaatgac agtcatacca agatttttgc
    19321 gggtaacttc cagcaatgcg tcacgcagac tggccgtttt cttaacatgc gggatctcat
    19381 cgcccgtatg cataatatcg tttacgcgca gcagaagttt acgacccagt gcgccgcctg
    19441 ggtgtgagag cgcaaaatct tcagcagtaa agccgcgtgc ttttaacagc gcgacagcga
    19501 gggcatcgcc cataaccagc gtggcggtgg tgctgctggt cggtgccagc cctaacggac
    19561 aggcttcttt cgctacttta acacacagat gcacatctgc ggcgcgcgcc atgctgctct
    19621 ccgggcgacc ggtgatgcag attaacggta cgtgaagacg cttaagcact ggaattaagg
    19681 ccgtgatttc gctggattca cctgagttag agatagcaat caccacatcc tgtggggtga
    19741 ccatgcctaa atcaccatgc gcggcttcac taggatggac gaaaaatgaa ggtgtaccgg
    19801 tgctggcaaa cgttgcggcc attttgcgcc cgatgtgccc cgatttcccc atccccatga
    19861 cgacaacttt ccctttacac cagaacatct tttcacaggc aagcgtgaaa ttctgattga
    19921 tgtattgatc aagctccgcc aggcattcac gttcaatcgc caggacttct ttacctgctt
    19981 gctgaaagtc aaaacccggt tgtaactcta cgtgcgacat aatgcgtttc cagttattca
    20041 acgagtattg gcgataacca gtacaacatc gccagccata cgataaatcc gccagttaat
    20101 aataccccta caccacggcc cggttgcggg gagcgccgcc agcacagcaa cgcaaaaata
    20161 atgctcacca gcaacatcac gctgtagtca cgactgtacg ccagtggatc aatctctcct
    20221 ggcgttatca gcgcgggtaa acccaacacg atgacaatat taaaaatgtt tgcgccaatg
    20281 atatttccga cagcaatgtc gttttcacct ttgcgaaccc ccgctattgc ggttgccagt
    20341 tccggcagac tggttccgat agcaattgcc gtcagagcca tcgtcaactc gctgatggca
    20401 aagtaattcg ccagcaccgt ggcgttatca accaccatcc gcgtggccac tggcatgatg
    20461 ataagcgcaa tgccgagcca taaaaacgcg acgggcaatc cgccgtcacg cggcagctct
    20521 gcaagctgct ctctggtcag gctgtcagtc ccctgacgtg caagtttaac aatgaacagc
    20581 agccatagca cagccagaaa gaggagaaag ataccatcgc ttcgactaag ttgtccgtca
    20641 tagagtacgg aaccggccac cacgctgacc aacaacatta agggtaattc acggcgcaga
    20701 acatcggaat ggacggtaaa aggacgaacc agcgcagcca gaccgaggat cagcaatata
    20761 ttgataatgt ttgagccgag ggctgtacca acggctaaat cgcgttgttc gtgcagagac
    20821 gcggcaagcg agacgatgat ttctggtaac gatgtaccaa tactgaccac cgtcatgccg
    20881 atgatcagcg gcgggatgcc aaaggttcgg caaagaatag acgcggcaaa aaccaggcgg
    20941 tcggcactgt aaacgaccaa aagtaaacca acaattaaca gtgccgtagc taaaagcatc
    21001 taacgtcctt tcttcaggta tactcgccgg tccgctgaag attttcagaa agccgtaacg
    21061 gattcttaat tttgacttta tgcggctaaa aagtaaaaca aatgccagct ttcgctaacc
    21121 acggcgggta atattctgta aatatgttgg gttcaaggtt aaattgagcg ccatgcttag
    21181 aaaatcaacg caagacgaag ggtgaattat ggagcagtct gtggcgaatt tagtcgatat
    21241 gcgcgatgtc agttttacgc gtggcaatcg ctgcatcttc gataatattt ccctgaccgt
    21301 gccgcgaggg aagatcacgg cgatcatggg gccatcgggc atcggtaaaa cgacgctact
    21361 ccgtctgatt ggcgggcaaa tcgcaccaga tcatggtgag atccttttcg atggtgagaa
    21421 tattccggcg atgtctcgtt cgcgcctgta tacagtgcgt aaacggatga gcatgttatt
    21481 tcagtccggg gcgttgttca ctgatatgaa cgtatttgac aacgtcgcct atccactgcg
    21541 cgaacatacc caacttcccg cgccattgtt gcatagtacg gtgatgatga agctggaggc
    21601 cgtggggctg cgtggagcgg ctaaactaat gccttctgaa ctttccggtg ggatggcgcg
    21661 gcgtgcagcg ctggcgcgtg cgattgcgct ggagccggat ctcatcatgt ttgatgaacc
    21721 ttttgttggg caagatccca tcaccatggg cgtactggtg aagctgattt ctgagctgaa
    21781 cagcgcgctg ggcgtgactt gtgtggtggt ttctcacgat gtgccggaag tgttaagtat
    21841 tgcggatcac gcctggatcc tggcggacaa aaaaattgtc gctcatggca gtgcccaggc
    21901 gttgcagacg aatcctgatc cgcgcgtacg tcagtttctg gacgggatag ctgacgggcc
    21961 tgttccgttc cgctatcctg ccggcgatta tcacgctgat cttttaccag ggagttaagc
    22021 cactcatgct gttaaatgcg ctggcgtcgc tcggacataa agggattaaa accctgagaa
    22081 cgttcgggcg ggccgggtta atgttattca atgcgctggt cggcaaaccg gaatttcgca
    22141 aacatgcgcc gctgctggtg cgccagctct ataatgtcgg cgtcctgtcg atgctgatta
    22201 ttgtagtttc tggcgtgttc atcggaatgg tgttggggct gcaaggttat ctggttctga
    22261 ccacttatag cgcggaaacc agtctgggta tgctggtggc gttatcgcta ctgcgtgaac
    22321 tggggccggt ggttgccgcg ctgttgtttg ccgggcgtgc tggttcggcg ttaaccgcag
    22381 aaatcggcct gatgcgcgct acagagcaac tctccagtat ggagatgatg gcggtggatc
    22441 cgctgcgtcg ggttatttct ccccgtttct gggctggggt tatttcatta ccactgttga
    22501 cggttatctt cgtcgccgtg gggatctggg gcggatcgct ggtcggcgtc agttggaaag
    22561 gcattgatag cgggttcttc tggtcggcaa tgcaaaatgc cgtcgactgg cgtatggatc
    22621 tggtcaactg tctgattaag agcgtggtgt tcgccatcac ggtgacgtgg atttcgttgt
    22681 ttaacggcta cgacgccatc ccgacgtctg ccgggattag ccgggcaacc actcgcaccg
    22741 ttgtccactc gtctctggct gttctggggc tggattttgt gctgaccgca ttgatgtttg
    22801 ggaattgagt tcatgcaaac gaaaaaaaat gaaatttggg tgggtatctt tttattagca
    22861 gcactgctgg cggcgctgtt tgtttgcctg aaggcggcga acgtgacgtc catacgtact
    22921 gaatcgacct acacgcttta tgcgacgttc gataacattg gcggcctgaa agcgcgctct
    22981 ccggtcagca ttggtggtgt tgttgtgggc cgggtggcgg atattacgct ggacccgaaa
    23041 acctatctgc cgcgcgtaac gctggaaatt gaacaacgtt ataaccacat tcctgatacc
    23101 agttcgctga gcattcgtac ttccggcctg ctgggagaac aatatctggc attaaacgtc
    23161 ggttttgaag acccggaact ggggactgct atcctgaagg atggcgatac aattcaggac
    23221 accaagtctg cgatggtgct ggaagatctc attggtcagt tcctttacgg tagtaaaggc
    23281 gatgacaata agaatagtgg cgatgcgcca gctgctgcgc caggtaataa tgaaaccact
    23341 gaacctgtgg gtacaacgaa ataatttcag gagaaccgac gcatgtttaa acgtttaatg
    23401 atggtcgctt tgctggtgat tgcacctctg agtgcggcaa ccgcggcaga ccagaccaat
    23461 ccttataagc tgatggacga ggcggcgcag aaaacgttcg atcgcctgaa gaatgagcaa
    23521 ccgcaaattc gggccaaccc ggattatctg cgtaccattg ttgatcagga actgctgcca
    23581 tacgtacagg tgaaatacgc cggtgcgctg gtgctgggcc agtattacaa gagtgcgacc
    23641 cctgctcaac gtgaagccta ctttgccgct ttccgtgagt acctgaagca ggcttacggt
    23701 caggcgctgg cgatgtatca cggtcaaacc tatcagattg cgccagaaca gccgctgggc
    23761 gataaaacca ttgtgcctat tcgcgttacc attattgacc cgaatggccg tccgccggtg
    23821 cgtctggact tccagtggcg taaaaactcc cagacgggaa actggcaagc ttacgacatg
    23881 attgctgaag gcgtcagtat gatcaccacc aaacaaaacg agtggggaac gctgctgcgt
    23941 accaaaggta tcgacggcct gactgcgcaa ctgaaatcga tttctcaaca gaaaatcact
    24001 ctggaagaga aaaaataatg agcgagtcac tgagctggat gcagacgggt gacacgctgg
    24061 cgttatccgg agagctggat caggacgttc tgctaccgct ttgggaaatg cgtgaggagg
    24121 cggtgaaggg gattacctgc atcgatctta gccgtgtgtc ccgcgtggat acggggggac
    24181 tggcactgct gctccatctt atcgatctgg cgaaaaagca gggcaacaac gtgacgcttc
    24241 agggggtaaa cgacaaagtg tataccctcg cgaaattgta taatttgcct gctgatgttc
    24301 tgcctcgtta aattttttca ggccggatat ggcgttaacc gcctgtccgg caaacctgcc
    24361 tgcaagtcga gccttgtctg atttatcagc gaggcttttt gcttatttat gccagcgcaa
    24421 ctttgctcta agatgtttcg ctggtttatt aactgatgat tgaagatccc atggaaaata
    24481 atgaaattca gagcgtgttg atgaacgctc tctccctcca ggaagtccac gtttccggcg
    24541 atggcagcca ctttcaggtt attgccgtgg gtgagttgtt tgacggtatg agtcgggtta
    24601 aaaaacagca gacggtctat ggcccgctga tggaatatat tgcggataac cgcattcatg
    24661 ctgtgtcgat caaagcgtat acccctgcgg agtgggcgcg cgatcgcaaa ctgaacggct
    24721 tttgagctat gggcgattcg ccggtagcgg atatgaattg ttaactgaga acaaactaaa
    24781 tggataaatt tcgtgttcag gggccaacga agctccaggg cgaagtcaca atttccggcg
    24841 ctaaaaatgc tgctctgcct attctttttg ccgcactact ggcggaagaa ccggtagaga
    24901 tccagaacgt cccgaaactg aaagacgtcg atacatcaat gaaactgcta agccagctgg
    24961 gtgcgaaagt agaacgtaat ggttctgtgc atattgatgc ccgcgacgtt aatgtattct
    25021 gcgcacctta cgatctggtt aaaaccatgc gtgcttctat ctgggcgctg gggccgctgg
    25081 tagcgcgctt tggtcagggg caagtttcac tacctggcgg ttgtacgatc ggtgcgcgtc
    25141 cggttgatct acacatttct ggcctcgaac aattaggcgc gaccatcaaa ctggaagaag
    25201 gttacgttaa agcttccgtc gatggtcgtc tgaaaggcgc acatatcgtg atggataaag
    25261 tcagcgttgg cgcaacggtg accatcatgt gtgctgcaac cctggcggaa ggcaccacga
    25321 ttattgaaaa cgcagcgcgt gaaccggaaa tcgtcgatac cgcgaacttc ctgattacgc
    25381 tgggtgcgaa aattagcggt cagggcaccg atcgtatcgt catcgaaggt gtggaacgtt
    25441 taggcggcgg tgtctatcgc gtgctgccgg atcgtatcga aaccggtact ttcctggtgg
    25501 cggcggcgat ctctcgtggc aaaattatct gccgtaacgc gcagccagat actctggacg
    25561 ccgtgctggc gaaactgcgt gatgctggag cggacatcga agtcggcgaa gactggatta
    25621 gcctggatat gcatggcaaa cgtccgaagg ctgttaacgt acgtaccgcg ccgcatccgg
    25681 cattcccgac cgatatgcag gcccagttca cgctgttgaa cctggtggct gaagggaccg
    25741 ggttcatcac cgaaacggtc tttgaaaacc gctttatgca tgtgccagag ctgagccgta
    25801 tgggcgcgca cgccgaaatc gaaagcaata ccgttatttg tcatggtgtt gaaaaacttt
    25861 ctggtgcaca ggttatggca accgatctgc gtgcatcagc aagcctggtg ctggctggct
    25921 gtattgcgga agggacgacg gtggttgatc gtatttatca catcgatcgt ggctacgaac
    25981 gcattgaaga caaactgcgc gctttaggtg caaatattga gcgtgtgaaa ggcgagtaat
    26041 cgtctgagag ctggcagccc cgttcgcggg gctaccgcca gaccactcat tttttcggtt
    26101 tagtgtagct acgcatcaac tgcgttctgt cgataaactc atgggtctgc ggatcatggt
    26161 agcgtgatgg ccagataacc caggggtcag ttcccagggc tttcgcaata atcatctctc
    26221 ctttcggcca tgggcgcgat aatgcgtttg ccagcgttga ggaactcaaa ccatttctgc
    26281 gagattccgc cgccattgac gttcctttct tgcgcaaacc tgcaatgatg tcagcgggat
    26341 gccagtcaat gaaattactt tccatctttc ctcactcctt aagttagtcg ctttcattca
    26401 gaacacttct tctgtcgggc atcactatac tcctgatggt ctattgcgct aaaaagttcc
    26461 tgtaaagagt gtgaatattc agaatatata aagattcatc gccctttttt caggtgttat
    26521 agcgctgaat tagtgttttt atggaattta ctggaatcat cccgcgcact gcgcgggatg
    26581 aagggagggg attaacgatc gcgttgaaca gcgatgtgcg cgaggccgat gagtgcttct
    26641 cgccaagggg tgtccgggag cacctgtaac gctgcgatgg ctttgtctgc ttcttcctcg
    26701 gcgcgctgac gcgtccattc aagagatcca caagcgttca ttgcttccag aaccggttcc
    26761 agaagatggc gaccgttacc ttgttcgatg gcggtacgga tcatctgtgc ctgttctggt
    26821 gtgccatgat gcatcgcatg cagcagcggc agcgtcggtt taccttcgtt cagatcgtct
    26881 ccgacgtttt tacctaactg ttcgccatcg gcattgtaat cgagtaaatc gtcgatcaac
    26941 tggaaagcag tgccgagata gcgcccataa tcctgcaggc ctttctcctc ctccggcgta
    27001 cagccagcca gaatcccgga acactgcgcg gcagcctcaa acagacgcgc ggttttgcta
    27061 tagataacgc gcatgtagtt ttcttcagtg atgtccggat cgttaacgtt catcagttgc
    27121 agaacttcac cttctgcgat gacgtttaca gcttctgaca tgacttccag cactttgagt
    27181 gaaccgaggc tggtcatcat ctggaacgcg cgggtataaa taaaatcgcc taccagcacg
    27241 ctggcggcat tgccaaatgc ggcgttggcg gtagctttac ccctgcgcat atctgattca
    27301 tccacaacgt cgtcgtgtag cagagtcgcc gtgtggataa actcgatcag ggcagcaatg
    27361 gtgacatgcg catttccctc atagccaaca gctcgtgcag ccagtacagc aatcatcgga
    27421 cgaatacgtt taccgccgcc gctgacgatg taatagccta actgattgat cagttggacg
    27481 tcggaattaa gctgctcaag gattgccgca ttaacacccg ccatatcttg cgcggttaac
    27541 tcattgattt tttctaaatt catcgcaaaa gccgggcttt tcgccccgat taccttactg
    27601 tgaacaaagg caacttaggg tttatggtta ggaatatagg gtaattgtac tgaaaaaatg
    27661 gcacagataa acgttaccgt acaagttgtg tttttttctt cgtgtattga ctgtagcact
    27721 tgtcaaaggc gtgcgttttg cgtaatattc gcgccctatt gtgaatattt atagcgcact
    27781 ctgaatcatt gaaaaggtgt gcgcggaagc ggagttttat atgtacgcgg ttttccaaag
    27841 tggtggtaaa caacaccgag taagcgaagg tcagaccgtt cgcctggaaa agctggacat
    27901 cgcaactggc gaaactgttg agttcgctga agtgctgatg atcgcaaacg gtgaagaagt
    27961 caaaatcggc gttcctttcg ttgatggcgg cgtaatcaaa gctgaagttg ttgctcacgg
    28021 tcgtggcgag aaagttaaaa tcgttaagtt tcgtcgtcgt aaacactatc gtaagcagca
    28081 gggccatcgt cagtggttca ctgatgtgaa aattactggc atcagcgcct aagacctgag
    28141 gagagatttc aaatggcaca taaaaaggct ggcggctcca cacgtaacgg tcgcgattca
    28201 gaagctaaac gcttgggcgt taagcgtttc ggtggcgaat ccgttctggc gggtagcatc
    28261 atcgttcgtc aacgtggtac caaattccac gctggcgcta acgtaggttg cggtcgtgac
    28321 cacactctgt ttgctaaagc agacggtaaa gtgaaattcg aagttaaagg cccgaaaaac
    28381 cgtaaattta tcagcatcga agctgaataa gtttttcgcg tcccggtaac ggatgaaagc
    28441 cccgcaacac gttgcggggc tttttacatt cgataaccgg taaaaatgcg catggtgcga
    28501 cgttaatccg caaatgcgct ttgcagggaa tacggtatga agcagcaggc aggcattggc
    28561 attcttttgg cgcttaccac agcaatttgc tggggggcgt tgccaatcgc aatgaagcag
    28621 gtgctggagg tgatggaacc tccgacaatc gtgttttacc gtttcttgat ggcgagtatt
    28681 ggccttggtg ccattcttgc ggtgaagaag aggttgccgc cattacgcgt gtttcgtaag
    28741 ccacgctggt tgattttgtt ggcagtggcg accgccgggc tgtttgggaa cttcatcctg
    28801 ttcagctcat ccttgcaata cctgagtccg accgcttcgc aggtgattgg gcaactctcg
    28861 ccagttggca tgatggttgc cagcgtattt atcctgaaag agaaaatgcg cagcactcag
    28921 gttgtagggg cattgatgct cctgagcggt ctggtgatgt tttttaacac cagtctggtc
    28981 gagatattta caaagctcac cgattacacc tggggagtta tctttggggt cggtgcggcg
    29041 acggtttggg tgagttatgg cgtggcgcaa aaggttttat tgcgtcggct ggcctcaccg
    29101 cagatcctgt ttttactgta cactttatgt acaatcgcgc tcttccctct ggcaaagcct
    29161 ggagtgatag cgcagcttag ccactggcag ctcgcatgtt taattttttg cggactgaat
    29221 accttggtag gatatggcgc cttggcggaa gcgatggctc gctggcaggc agcgcaggtg
    29281 agcgcgatca tcacgctcac cccactgttt acgctgtttt tttcagatct tttatcactg
    29341 gcctggcccg atttcttcgc cagaccgatg ttaaaccttt taggttatct cggtgcgttt
    29401 gtcgtggttg cgggcgcgat gtattccgcc attggtcatc gtatttgggg cggattacgt
    29461 aagcatacaa cggtggtatc gcaaccccgc gcaggcgaat gatttacgga gaataaaatg
    29521 aagtttgttg atgaagcatc gattctggtc gttgcaggtg atggcggtaa tggttgcgtg
    29581 agcttccgcc gcgaaaagta tattccgaaa ggcggcccgg atggcggcga cggcggtgat
    29641 ggtggtgacg tatggatgga agccgacgag aacctgaaca cgcttatcga ttatcgtttt
    29701 gaaaaatctt tccgtgcaga gcgcggtcag aacggcgcaa gccgcgattg taccggtaag
    29761 cgcggtaaag acgtgacgat taaagtgccg gtaggtacgc gtgtaatcga ccagggtact
    29821 ggtgaaacca tgggtgatat gactaaacac ggtcagcgtc tgctggttgc taagggcggc
    29881 tggcacggtc tgggcaatac ccgtttcaaa tcgtcagtta accgtacacc acggcagaaa
    29941 actaacggta cgccgggcga taagcgtgag ctgctgctgg agctgatgct gctggctgac
    30001 gtcggtatgt tggggatgcc aaacgcgggt aaatcgacct ttattcgtgc ggtatcggcg
    30061 gctaaaccga aagtggcgga ttatccgttt accactctgg tgccaagtct gggtgtggta
    30121 cgaatggaca acgaaaagag cttcgttgtt gccgatattc caggactgat tgaaggcgct
    30181 gcggaaggcg caggtctggg cattcgcttc ctgaagcacc tggaacgttg ccgcgtcctg
    30241 ttgcacctca tcgatatcga tccgattgac ggcaccgatc cggttgaaaa cgcgcgtatt
    30301 attatcagcg agctggaaaa atacagccag gatctggcgg cgaaaccgcg ttggttagtc
    30361 ttcaacaaga tcgatctgct ggataaggca gaagccgaag agaaagcgaa agcgatcgct
    30421 gaagcgctgg gctgggaaga taaatattat ctgatctctg cggcgagcgg actgggcgtg
    30481 aaagatctct gctgggatgt gatgaccttt atcattgaaa acccggtcgt gcaggctgaa
    30541 gaagcgaaac agccagagaa agtcgaattc atgtgggatg attatcaccg tcagcagctt
    30601 gaagagattg ctgaagagga tgatgaagac tgggatgacg actgggacga agacgacgaa
    30661 gaaggcgttg agttcattta caagcgttaa tcactaatga ccaatgccaa ataagcctga
    30721 tgcgctacac ttatcaggcc tacaagatga ttgcaatata ttgaatttgc acgattttgt
    30781 aggccggata aggcgttcac gccgcatccg gcataaacaa agcgcacttt gtcaataatc
    30841 tgaaaccccg gccatgcgcc ggggtttctt tttgactaat tgttctgata aatatctttg
    30901 tacaaacggc tttcaaaacg cactaacgga atacggcgat tacgctgatc cgcgggtttt
    30961 actgcatagc cagaaagata ttgtacaaat gccattcgtt gcccgctcgc tgtggtaatg
    31021 aatcccgcca ggttatatac cccctgcaac gaaccggttt tcgctgagac tttgccatcc
    31081 acgcccgcct gatgcagacc tgcacggtac tgcaaagagc cgtcatagcc cgcaagcggc
    31141 agcatggaga taaagttaag ttcattgtcg tgttgggcaa tgtattgcag cacctgcatc
    31201 atggtggcgg gggcaatcag gttatgccgc gaaagccctg aaccatcggc aataatggtg
    31261 ttaccaatat cgacaccggc ttgctggcgc aggatctgac gcacggcgtc cgacccagcc
    31321 cgccatgttc caggcacatt gaagcgcgca tggccgatca tgcggaaaac cgtatcggcg
    31381 atcatgttgt ccgacttttt cagcataatc ttaagcagat cgtgcagcgg ggccgactgt
    31441 ttactggcaa ctaccgttcc aggttcgtta acctgagtct ggcgcagcag tgttccgctc
    31501 caggtgatac ccgcctgttt taactcatct ttcagaattg caccggcata gctggctcca
    31561 tcctgcacgg caaaagccaa cgggagcggc tcagaacgtt gtggcaggca tcccgtcagc
    31621 gtaaagcggt tcaggtcgct tggcaccaca tccagttcgc agtattgcgc ttcggcagaa
    31681 ccacggggga gggtgcgtac ctggctgaac atcgtaacgg ggtaataaga tgccacgcgt
    31741 ataaaagcca tatcaccagg ctttggggca ctgtagagcg agacggagaa acagttgcgg
    31801 tcaactatgg cggcagcagg cggagcgcta aagcattgtg tcatgtcatt ccatggccag
    31861 ccgggggctt tatcgtggct ggcgaaaatg gaggtatcta tcaacacatt gccatcgatt
    31921 tggttgacgc cagatttttt caaagtcgcg accatattgc gaatatcctg acgttttaac
    31981 gtcggatcgg caccaaatcg cgccactaag tcacccttaa gtacgccgtt ttccacattg
    32041 cctttggttt caagcgtcgt ggtgaaacga aaatcggggc cgagttgaat caacgccgcc
    32101 agcgcagtaa tcactttctg ggtactggcg ggcagcgcca tctgctgact gtggtaatca
    32161 atagcggggg ccgacgcacc gactttttgc accatcaggg caaggttggc acctgcgggg
    32221 agttgagtaa cgtactcatc aacattcgcg gcctgaacac tgaacgctat acagctggtc
    32281 aatccgatga taaatctgga aaatcgcata atctcgcgct aacaacctgg aatcgagccg
    32341 tcatactacg gcgcaacgcc ctataaagta aacgatgacc cttcgggaac ttcagggtaa
    32401 aatgaccatc aaaatgtgaa ttgtagctga cctgggactt gtacccgggt cggtattttt
    32461 ttgcttctgg tcccggtgag gagttatgcc gggcaggccg aacagccggg gtgggtgaag
    32521 acttgcccca tcaggaatat tcaagaggta taacaaatgc aagctattcc gatgacctta
    32581 cgcggcgctg aaaaattacg cgaagagctg gattttctga aatctgtgcg ccgtcctgaa
    32641 atcattgctg ctatcgcgga agcgcgtgag catggcgacc tgaaagaaaa cgccgaatac
    32701 cacgcagctc gtgaacagca gggcttctgc gaaggccgta tcaaagacat cgaagccaag
    32761 ctgtcaaacg cgcaggtgat tgatgtcacc aaaatgccca acaatgggcg cgttattttt
    32821 ggtgctaccg taacggtgct gaatctggat tctgacgaag aacagactta tcgcatcgtt
    32881 ggcgatgacg aagctgactt taaacaaaac ctgatttctg taaactcgcc tattgctcgt
    32941 ggcctgatcg gcaaagaaga agatgatgtt gtggtcatca aaacgccggg cggcgaagta
    33001 gaatttgaag taattaaggt ggaatacctg taagaattac ccaatactca agatgttgat
    33061 gtattgtaaa gaaaggaaaa aggccgctct gcggcctttt atcaacgaac agagcgtggc
    33121 attttgctct cctgcctgcg gaaaacccct cgttttacac agcaaatgtg tgtaacttta
    33181 ggataatctt agcgtggcag cgagatttta cgttctttag ttgggcgata aagcaccagc
    33241 gttttaccga tgacctgtac attacaggcg ccggtttcgc gcacgatagc ttccacgatc
    33301 aaggtttttg tttcgcgatc ttcggtggcg attttcacct tgatgagttc atggtgctct
    33361 aacgcttgtt caatctcggc cagcacccct tcggtcaaac cattactgcc aagcagaaca
    33421 actggcttga gcggatgtgc cagacctttc aggtgctgtt tttgtttagt actcagattc
    33481 atcgtatttt ttgcttacgt tgggattgaa aacgggtcat tctaccgcca tctcccatat
    33541 atcaccaaat aggcgcgtaa aaatttacgc aattggttac gatgagttat ccccatggga
    33601 aagttaaatg acaggtaaga agcgttctgc cagctccagc cgctggcttc aggaacactt
    33661 tagcgataaa tatgttcaac aggcacagaa aaagggatta cgttcccgtg cctggtttaa
    33721 acttgatgaa atacagcaaa gtgacaaact ctttaaaccg ggaatgacgg ttgtcgacct
    33781 tggtgctgct ccgggtggtt ggtcacaata tgtggtcacc caaattggcg gcaaaggccg
    33841 catcatcgct tgcgatcttt tacctatgga tcctatcgtt ggtgtggact ttcttcaggg
    33901 cgattttcgt gatgaactgg tgatgaaagc actgctggag cgcgttggcg acagcaaagt
    33961 ccaggttgtc atgtccgata tggcaccaaa catgagcgga acaccggcgg tggatatccc
    34021 ccgagccatg tatctggtgg aactggcgct agaaatgtgt cgtgatgtat tagcgccagg
    34081 tggcagtttt gtagtgaagg tgttccaggg cgaaggtttc gatgagtatc taagggaaat
    34141 tcgctccctg tttacgaagg tcaaagttcg taagccggac tcttctcgtg cacgttcgcg
    34201 agaagtgtat attgtagcga ccgggcgtaa accctaaccg ggagatttca gacgaaagtt
    34261 tgaaagatgc tggatataga gtatcctgac gctgttttta acacagttgt aataagaggt
    34321 taatcccttg agtgacatgg cgaaaaacct aatactctgg ctggtcattg ccgttgtgct
    34381 gatgtcagta ttccagagct ttgggcccag cgagtctaat ggccgtaagg tggattactc
    34441 taccttccta caagaggtca ataacgacca ggttcgtgaa gcgcgtatca acggacgtga
    34501 aatcaacgtt accaagaaag atagtaaccg ttataccact tacattccgg ttcaggatcc
    34561 gaaattactg gataacctgt tgaccaagaa cgtcaaggtt gtcggtgaac cgcctgaaga
    34621 accaagcctg ctggcttcta tcttcatctc ctggttcccg atgctgttgc tgattggtgt
    34681 ctggatcttc ttcatgcgtc aaatgcaggg cggcggtggc aaaggtgcca tgtcgtttgg
    34741 taagagcaaa gcgcgcatgc tgacggaaga tcagatcaaa acgacctttg ctgacgttgc
    34801 gggctgcgac gaagcaaaag aagaagttgc tgaactggta gagtatctgc gcgagccgag
    34861 ccgcttccag aaactcggcg gtaagatccc gaaaggcgtc ctgatggtcg gtcctccggg
    34921 taccggtaaa acgttgctgg cgaaagcgat tgcaggcgaa gcgaaagttc cgttctttac
    34981 tatctccggt tctgacttcg tagaaatgtt cgtcggtgtg ggtgcatccc gtgttcgtga
    35041 catgttcgaa caggcgaaga aagcggcacc gtgcatcatc tttatcgatg aaatcgacgc
    35101 cgtaggccgc cagcgtggcg ctggtctggg cggtggtcac gatgaacgtg aacagactct
    35161 gaaccagatg ctggttgaga tggatggctt cgaaggtaac gaaggtatca tcgttatcgc
    35221 cgcgactaac cgtccggacg ttctcgaccc ggccctgctg cgtcctggcc gtttcgaccg
    35281 ccaggttgtg gtcggcttgc cagatgttcg cggtcgtgag cagatcctga aagttcacat
    35341 gcgtcgcgta ccattggcac ccgatatcga cgcggcaatc attgctcgtg gtactcctgg
    35401 tttctccggt gctgacctgg cgaacctggt gaacgaagcg gcactgttcg ctgctcgtgg
    35461 caacaaacgc gttgtgtcga tggttgagtt cgagaaagcg aaagacaaaa tcatgatggg
    35521 tgcggaacgt cgctccatgg tgatgacgga agcgcagaaa gaatcaacgg cttaccacga
    35581 agcgggtcat gcgattatcg gtcgcctggt gccggaacac gatccggtgc ataaagtgac
    35641 gattatccca cgcggtcgtg cgctgggtgt gactttcttc ttgcctgagg gcgacgcaat
    35701 cagcgccagc cgtcagaaac tggaaagcca gatttctacg ctgtacggtg gtcgtctggc
    35761 agaagagatc atctacgggc cggaacatgt ttctaccggt gcgtccaacg atattaaagt
    35821 tgcgaccaac ctggcacgta acatggtgac tcagtggggc ttctctgaga aattgggtcc
    35881 actgctgtac gcggaagaag aaggtgaagt attcctcggc cgtagcgtag cgaaagcgaa
    35941 acatatgtcc gatgaaactg cacgtatcat cgaccaggaa gtgaaagcac tgattgagcg
    36001 taactataat cgtgcgcgtc agcttctgac cgacaatatg gatattctgc atgcgatgaa
    36061 agatgctctc atgaaatatg agactatcga cgcaccgcag attgatgacc tgatggcacg
    36121 tcgcgatgta cgtccgccag cgggctggga agaaccaggc gcttctaaca attctggcga
    36181 caatggtagt ccaaaggctc ctcgtccggt tgatgaaccg cgtacgccga acccgggtaa
    36241 caccatgtca gagcagttag gcgacaagta agttcccgca tcagatgact gtatttgtac
    36301 cgaaaacccc ggggcgtgct ccggggtttt ttcttatcaa ttcataccag ggataacata
    36361 atgaaactct ttgcccaggg tacttcactg gaccttagcc atcctcacgt aatggggatc
    36421 ctcaacgtca cgcctgattc cttttcggat ggtggcacgc ataactcgct gatagatgcg
    36481 gtgaaacatg cgaatctgat gatcaatgct ggcgcgacga tcattgacgt tggtggcgag
    36541 tcca
//
LOCUS       DAJTRW010000032        35749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRW010000032 DAJTRW010000000
VERSION     DAJTRW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..237
                     /locus_tag="OO326_001745"
     CDS             <1..237
                     /locus_tag="OO326_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5966156.1"
                     /translation="PRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQR
                     PLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(284..1042)
                     /gene="deoR"
                     /locus_tag="OO326_001746"
     CDS             complement(284..1042)
                     /gene="deoR"
                     /locus_tag="OO326_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5966157.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1100..1696)
                     /gene="ybjG"
                     /locus_tag="OO326_001747"
     CDS             complement(1100..1696)
                     /gene="ybjG"
                     /locus_tag="OO326_001747"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5966158.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1981..3207
                     /gene="mdfA"
                     /locus_tag="OO326_001748"
     CDS             1981..3207
                     /gene="mdfA"
                     /locus_tag="OO326_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5966159.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3248..3532)
                     /locus_tag="OO326_001749"
     CDS             complement(3248..3532)
                     /locus_tag="OO326_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966160.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3618..4433)
                     /gene="ybjI"
                     /locus_tag="OO326_001750"
     CDS             complement(3618..4433)
                     /gene="ybjI"
                     /locus_tag="OO326_001750"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5966161.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4433..5641)
                     /locus_tag="OO326_001751"
     CDS             complement(4433..5641)
                     /locus_tag="OO326_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966162.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5725..6261
                     /gene="rcdA"
                     /locus_tag="OO326_001752"
     CDS             5725..6261
                     /gene="rcdA"
                     /locus_tag="OO326_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5966163.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6436..8121)
                     /locus_tag="OO326_001753"
     CDS             complement(6436..8121)
                     /locus_tag="OO326_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5966164.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8391..8768
                     /locus_tag="OO326_001754"
     CDS             8391..8768
                     /locus_tag="OO326_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5966165.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8798..9055)
                     /gene="grxA"
                     /locus_tag="OO326_001755"
     CDS             complement(8798..9055)
                     /gene="grxA"
                     /locus_tag="OO326_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5966166.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9215..9502
                     /locus_tag="OO326_001756"
     CDS             9215..9502
                     /locus_tag="OO326_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5966167.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9486..10208
                     /gene="nfsA"
                     /locus_tag="OO326_001757"
     CDS             9486..10208
                     /gene="nfsA"
                     /locus_tag="OO326_001757"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5966168.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10269..11171
                     /gene="rimK"
                     /locus_tag="OO326_001758"
     CDS             10269..11171
                     /gene="rimK"
                     /locus_tag="OO326_001758"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5966169.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11259..11735
                     /locus_tag="OO326_001759"
     CDS             11259..11735
                     /locus_tag="OO326_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5966170.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12087..13199
                     /gene="potF"
                     /locus_tag="OO326_001760"
     CDS             12087..13199
                     /gene="potF"
                     /locus_tag="OO326_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5966171.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13294..14427
                     /gene="potG"
                     /locus_tag="OO326_001761"
     CDS             13294..14427
                     /gene="potG"
                     /locus_tag="OO326_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5966172.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14437..15390
                     /gene="potH"
                     /locus_tag="OO326_001762"
     CDS             14437..15390
                     /gene="potH"
                     /locus_tag="OO326_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5966173.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15387..16232
                     /gene="potI"
                     /locus_tag="OO326_001763"
     CDS             15387..16232
                     /gene="potI"
                     /locus_tag="OO326_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5966174.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16292..16780
                     /locus_tag="OO326_001764"
     CDS             16292..16780
                     /locus_tag="OO326_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5966175.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16821..17948
                     /gene="rlmC"
                     /locus_tag="OO326_001765"
     CDS             16821..17948
                     /gene="rlmC"
                     /locus_tag="OO326_001765"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5966176.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18123..18854)
                     /gene="artJ"
                     /locus_tag="OO326_001766"
     CDS             complement(18123..18854)
                     /gene="artJ"
                     /locus_tag="OO326_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5966177.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19146..19814)
                     /gene="artM"
                     /locus_tag="OO326_001767"
     CDS             complement(19146..19814)
                     /gene="artM"
                     /locus_tag="OO326_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5966178.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19814..20530)
                     /gene="artQ"
                     /locus_tag="OO326_001768"
     CDS             complement(19814..20530)
                     /gene="artQ"
                     /locus_tag="OO326_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5966179.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20537..21268)
                     /gene="artJ"
                     /locus_tag="OO326_001769"
     CDS             complement(20537..21268)
                     /gene="artJ"
                     /locus_tag="OO326_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5966180.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21286..22014)
                     /gene="artP"
                     /locus_tag="OO326_001770"
     CDS             complement(21286..22014)
                     /gene="artP"
                     /locus_tag="OO326_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5966181.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22232..22747)
                     /locus_tag="OO326_001771"
     CDS             complement(22232..22747)
                     /locus_tag="OO326_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5966182.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23396..25165
                     /locus_tag="OO326_001772"
     CDS             23396..25165
                     /locus_tag="OO326_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5966183.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25376..25699
                     /locus_tag="OO326_001773"
     CDS             25376..25699
                     /locus_tag="OO326_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5966184.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25696..26526
                     /gene="amiD"
                     /locus_tag="OO326_001774"
     CDS             25696..26526
                     /gene="amiD"
                     /locus_tag="OO326_001774"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5966185.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26523..27536)
                     /locus_tag="OO326_001775"
     CDS             complement(26523..27536)
                     /locus_tag="OO326_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5966186.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27635..29065)
                     /locus_tag="OO326_001776"
     CDS             complement(27635..29065)
                     /locus_tag="OO326_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5966187.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29076..30077)
                     /gene="ltaE"
                     /locus_tag="OO326_001777"
     CDS             complement(29076..30077)
                     /gene="ltaE"
                     /locus_tag="OO326_001777"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5966188.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30114..31832)
                     /gene="poxB"
                     /locus_tag="OO326_001778"
     CDS             complement(30114..31832)
                     /gene="poxB"
                     /locus_tag="OO326_001778"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5966189.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31965..32933)
                     /gene="hcr"
                     /locus_tag="OO326_001779"
     CDS             complement(31965..32933)
                     /gene="hcr"
                     /locus_tag="OO326_001779"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5966190.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32945..34597)
                     /gene="hcp"
                     /locus_tag="OO326_001780"
                     /gene_synonym="priS"
     CDS             complement(32945..34597)
                     /gene="hcp"
                     /locus_tag="OO326_001780"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5966191.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34741..35640)
                     /gene="lysO"
                     /locus_tag="OO326_001781"
     CDS             complement(34741..35640)
                     /gene="lysO"
                     /locus_tag="OO326_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5966192.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8909 a         8837 c         9189 g         8814 t
ORIGIN      
        1 ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc gcagcttacc
       61 gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa cggtaaatcc
      121 attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtagg
      181 atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct
      241 tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa
      301 tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca ctcaccacaa
      361 tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat ttactgtggt
      421 cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc ggcaactctt
      481 caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa taaaaagcga
      541 tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa atggcgttgc
      601 tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct ttcagcgcca
      661 gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca atcgcttcaa
      721 taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg
      781 tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt tgatcgctta
      841 acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc aaaacgacgg
      901 gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg
      961 cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc tgcccgatac
     1021 gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag ccgctcaata
     1081 ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt aatgcaaaac
     1141 agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct tgccagataa
     1201 tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata tccagcggcc
     1261 agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc attaaaagtg
     1321 agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac
     1381 cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag ccgatatttt
     1441 cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca aacaggctga
     1501 ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc ccccacaacc
     1561 aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata aaaatcgcca
     1621 acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat agagagagat
     1681 tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca atacaggcaa
     1741 gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta tttccctcaa
     1801 gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact taaagaaagc
     1861 gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg
     1921 ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg aagaaattgc
     1981 atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact tttccctctc
     2041 tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca acccggtatg
     2101 ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc gatgaccgcg
     2161 tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga tcgtattggt
     2221 cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct ggcaatatta
     2281 ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat aagcttctgt
     2341 ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc
     2401 aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg tccgctggtg
     2461 ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt tgccgcattg
     2521 gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat gcgtataggc
     2581 gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa gaacggccgc
     2641 tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat cgcccagtcg
     2701 ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt gctgcaagtg
     2761 cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac ctcgcgccgc
     2821 accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct gttggtcgct
     2881 gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg gttaagtatt
     2941 tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct gtttgccagc
     3001 gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct gatctttacc
     3061 gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact gtttaatctc
     3121 ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt aaaagataaa
     3181 cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc gatcaggcat
     3241 tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct tcacctacgc
     3301 tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca ggagccacat
     3361 agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact gaatcaggaa
     3421 ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc atcgccacac
     3481 cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc atttcgcctg
     3541 aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc attgtgttgc
     3601 cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac tttatcgatc
     3661 acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc tgcgacgact
     3721 gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat ctcaatatcg
     3781 ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa tttctgcagt
     3841 tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct gccgttgccg
     3901 gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg tacttgtgga
     3961 atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa gttgtcaaag
     4021 ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat ggcatcgtca
     4081 tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac ttccgggcgc
     4141 gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc attaaaaaca
     4201 tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat ttcattcgca
     4261 atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac cacaaagcga
     4321 atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt ataggttttt
     4381 tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct catcaactat
     4441 tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg acaatagccg
     4501 cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa tgttcgccga
     4561 gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt gctaccacac
     4621 tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc agcgggaagc
     4681 ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag gcgctatcga
     4741 caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga accacggcaa
     4801 cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc atccccaggg
     4861 taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca tcaaccatta
     4921 ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc actacaacac
     4981 caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca tgctgggtgc
     5041 catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata taaataggcg
     5101 cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca aaggccgtca
     5161 gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg tgcatcatcg
     5221 gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt atcgccactt
     5281 ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc aggggcgatg
     5341 tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac atcgtgacca
     5401 ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga ataccgctca
     5461 tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag acagagagaa
     5521 tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct ggcaaaaaga
     5581 agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa tttacggtca
     5641 tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt acatttgtac
     5701 acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg gcgagaaaaa
     5761 attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt tacgcaccgc
     5821 aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt ttcaggaatt
     5881 gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg gcaatatcag
     5941 gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac cgatatgatc
     6001 tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct ctacgcgctg
     6061 gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg cagtcagcaa
     6121 acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt tattgagggg
     6181 atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat tttgaggatg
     6241 gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca tcaaccttga
     6301 accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa agcagtggca
     6361 ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa tttcattttc
     6421 agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg tccctgccag
     6481 cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag ggatgttact
     6541 gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc ccatcattgc
     6601 gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga aacagataac
     6661 cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa tcgcgcccag
     6721 gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca ccatcaagcc
     6781 gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg ggtggttagc
     6841 acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc ccatgccgaa
     6901 actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa caaagaaggc
     6961 gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga tgcgatcggc
     7021 gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt tgttaagcac
     7081 gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa ggaagcaacc
     7141 gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt ttttaacgac
     7201 cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt ctttaccgtt
     7261 acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg ctatttcatc
     7321 gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac gtcgaatacg
     7381 ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt tgccgtcggt
     7441 ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat aaaccttacg
     7501 gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg tctgtaagtc
     7561 ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca gaccgattaa
     7621 ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct gcctgctttc
     7681 catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg acgtcataga
     7741 gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac ctaaccctaa
     7801 ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat ttttcccatc
     7861 gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga aaataaacag
     7921 cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc ctaataacag
     7981 cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc gtaactttcc
     8041 gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc catttaacaa
     8101 ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc tgttgaaaga
     8161 aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca ttcagcgcac
     8221 ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg tatcaacggc
     8281 tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat cttactgaac
     8341 gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga gtgaaacata
     8401 aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg tgcctttttc
     8461 tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc ggcttgctgt
     8521 ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa gtcctgaaac
     8581 ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg ctgttttttt
     8641 caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg ttctggtgtg
     8701 cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag cacagaaaaa
     8761 atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga ttttctttca
     8821 cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa atctgcggca
     8881 cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc ccttccgcac
     8941 gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc tcagccagat
     9001 cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt tgcattattt
     9061 ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct gaaagtaggt
     9121 ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca gtgaaaagtt
     9181 ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta aattgcctaa
     9241 aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc tgctgtcggt
     9301 aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga ttctgatgat
     9361 ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc aggtggcaaa
     9421 acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag aaagagaaaa
     9481 agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc catttcactg
     9541 atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt gcgacgtcca
     9601 gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca ttacgtgaag
     9661 aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag ttctgggtgt
     9721 tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc ggcctggcgg
     9781 aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca ttaaccgcag
     9841 cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat attgaagcgg
     9901 tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg tgccttggct
     9961 ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg gtgcatgaaa
    10021 acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa ctggcggaat
    10081 attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat atccgccgaa
    10141 caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag ggttgggcga
    10201 cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt ctgacagaga
    10261 ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg tgtaagcggc
    10321 tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg ctttcttgct
    10381 acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag ttaccccatt
    10441 ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg gcggcactgc
    10501 gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc
    10561 gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg cctgtcacgg
    10621 gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt ggtgcgccgc
    10681 tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg gagactcgtc
    10741 aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg
    10801 aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt gatgaagtgg
    10861 tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg catcgtggcg
    10921 gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata aaagccgcgc
    10981 gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc gggccgttgg
    11041 tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc ggtatcgata
    11101 tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat tgcctgaaaa
    11161 cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt ttgcgtaagc
    11221 tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg gtcgttcctg
    11281 gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt gaatgtgata
    11341 actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt gatgccaaaa
    11401 tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga ccttcagccg
    11461 tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat
    11521 ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct ttatccgtta
    11581 tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt
    11641 cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac gatgaagggc
    11701 aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc acgcagtcgc
    11761 tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc ttaaagatta
    11821 tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca taaaaatttg
    11881 ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt tattcttgcg
    11941 atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt acaccgcgcc
    12001 gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt gttcgtttgt
    12061 taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg ctatcgggtc
    12121 tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa caaaaaacac
    12181 tccacattta taactggtct gattatatcg cccctgacac ggtggccaat tttgaaaaag
    12241 aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg gaaggcaaat
    12301 taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt ctagaacgcc
    12361 agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag tggaagaatc
    12421 tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa tttgctatgc
    12481 cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa gctgtgctgg
    12541 gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat ctggaaaaac
    12601 tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct accgtgttga
    12661 attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga ccggcaaccg
    12721 atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa tacattaacg
    12781 acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc tggcaggcgt
    12841 caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt ccaaaagaag
    12901 gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac aaagacgaag
    12961 cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt tccgaccatg
    13021 tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa gtccgtgaga
    13081 acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg aaagtgcagg
    13141 acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc ggaaaataat
    13201 ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca ccatcatggt
    13261 gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc tcgcccgcag
    13321 gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac caaatcctac
    13381 gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga aatcttcgcg
    13441 ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc aggtttcgaa
    13501 caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt tccgccttac
    13561 ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat gaccgtggaa
    13621 cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat ttccagtcgg
    13681 gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa accgcatcag
    13741 ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa gcgcccgaaa
    13801 ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga ccggatgcag
    13861 cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt cacccacgat
    13921 caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg gaaatttgtc
    13981 cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag cgccgaattt
    14041 attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga tggcttggtg
    14101 cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc ggtggtcgat
    14161 aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga agagccgccc
    14221 gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct cggcgatctt
    14281 tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct acaaaacgcc
    14341 catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg ctgggaagtg
    14401 gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga acctgctgcc
    14461 cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat gaagcatggg
    14521 cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct gctgccattt
    14581 ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc ttataccgaa
    14641 ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa ttttctgcaa
    14701 ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt ggctgcgatt
    14761 tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc gcacagtaag
    14821 ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac ctcgtttctg
    14881 atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa taattttctg
    14941 ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct ggcagtttat
    15001 attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac cgcgttgatt
    15061 cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc gctgaaaacg
    15121 ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc gatgctggtg
    15181 tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc ggacagcatc
    15241 atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc ggtggcctcg
    15301 gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt ccacaaacac
    15361 cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt tcgccctggc
    15421 ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg atgctggtta
    15481 tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca acgcgctggt
    15541 atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc ctgacaattg
    15601 ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg gtgctggtgc
    15661 gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg ccgctagtaa
    15721 tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt gctcatgcca
    15781 ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc actttctgta
    15841 cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc tcgatagaag
    15901 aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt acgctaccga
    15961 tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg cttgatgatc
    16021 tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg ctggtctttt
    16081 ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg atcctcggcg
    16141 cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa aaacagcgga
    16201 tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca acctggctac
    16261 ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg gattttttaa
    16321 gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg tggcggcgct
    16381 cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc tgggcgtgcg
    16441 cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc tggtcttttt
    16501 aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg tgaaagggcg
    16561 tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt acttgtgggc
    16621 ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat caaaacgtga
    16681 aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga tgctgctgtt
    16741 cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat cgtcgcccct
    16801 gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc tgtcgttcct
    16861 gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc gatcttaaaa
    16921 atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc ccggaacaag
    16981 ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca ctgctcggta
    17041 tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat ccagcctcat
    17101 ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta acgccctaca
    17161 acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc cagagtgatg
    17221 gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa ctgcgtaagg
    17281 cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc aatattcagc
    17341 cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa caacaggcac
    17401 tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc ttccagacta
    17461 atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga cagctgccgg
    17521 ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac tgcgcgacgc
    17581 ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt gcaaagcagt
    17641 cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc actcagtttg
    17701 ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc cgcggcattg
    17761 gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc tactccagct
    17821 gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc atcgaacggg
    17881 tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg ctgctggtga
    17941 agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct ttgttcatgc
    18001 cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa tacattgcag
    18061 agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt catcagtctc
    18121 aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc tttaattgct
    18181 gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac agcgataccc
    18241 aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc cagttgcgga
    18301 ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc aatacgacca
    18361 tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt cacttccggg
    18421 tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat gcgtttgcct
    18481 ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc tgagttttca
    18541 tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc cataccggag
    18601 attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa cgcgtgatta
    18661 gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc gatatcaaag
    18721 ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc tgaaacgcca
    18781 aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa agcggcaaga
    18841 actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg ccgtgtctgc
    18901 ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat taaataattt
    18961 aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta taagaattta
    19021 tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac cgtgctgatg
    19081 tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga atgtacgcag
    19141 ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg catcatcagc
    19201 gtcagcaggc cgttgacgac caagtaaata atccctgccg caccgaacac cattacatcg
    19261 taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa
    19321 gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt
    19381 ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta
    19441 caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta
    19501 tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc tgacaacaaa
    19561 tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta ataaatcagg
    19621 aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac
    19681 accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc
    19741 acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat cagctcgggt
    19801 aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt
    19861 tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc
    19921 aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt aaatcattca
    19981 cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc
    20041 caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg
    20101 acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc aacgcgccgc
    20161 gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga
    20221 acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg aacccaagat
    20281 tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa tagataaaca
    20341 gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg
    20401 cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc gccagcccga
    20461 caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa
    20521 attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat
    20581 ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg tttccctgac
    20641 gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta tcgcccactg
    20701 ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca ccaaatacgc
    20761 tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg
    20821 tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg ttctgaacgc
    20881 cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca
    20941 gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc tccggagtga
    21001 tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg atcaggctgt
    21061 caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt gcctgcgcca
    21121 ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg
    21181 gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa
    21241 ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata
    21301 gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat
    21361 atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg
    21421 ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg
    21481 tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc
    21541 catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg
    21601 gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag
    21661 cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac
    21721 ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc
    21781 gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag
    21841 cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc
    21901 gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa
    21961 cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac
    22021 tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttaa cagaataatt
    22081 atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca
    22141 aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga aatgccggat
    22201 aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg
    22261 tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg acccaacact
    22321 ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt ttgagatcca
    22381 cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg gcaacgtggg
    22441 cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat tcacgatgac
    22501 tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa taggggcgca
    22561 gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt tgagccacac
    22621 tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta taagctggcg
    22681 tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat agtgaacggt
    22741 agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc aaacgctatc
    22801 agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac ctgattgggt
    22861 ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact taaattaccc
    22921 aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct gaaatgatca
    22981 tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag aggttaagag
    23041 catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg gttattgtct
    23101 aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg attgccaaaa
    23161 ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac tctcgataat
    23221 attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag tgaagaataa
    23281 attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg tgcttttata
    23341 attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt ttaatatgat
    23401 aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc aaagtgataa
    23461 tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta agggtgagtt
    23521 gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg acagtgatct
    23581 cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc aatttaatac
    23641 attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat cagaagataa
    23701 cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta atgtatcgaa
    23761 tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata aattacaaga
    23821 acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt ataccattgg
    23881 tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact ttaacaaaga
    23941 acaatatata tcctttataa aggacgcatt tattcatttt tatgattata gcaggcgata
    24001 tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat tgatgagcac
    24061 tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta cagaagaaga
    24121 gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg ctgatgataa
    24181 ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg cctttcaaga
    24241 taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata tttgtagcgt
    24301 aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga atgaagaagt
    24361 tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa gtaatttagt
    24421 caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt ttaatgacgt
    24481 ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg tgaatgaaag
    24541 catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa taacaaatag
    24601 caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa ttaataacgt
    24661 aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata tgataagagt
    24721 taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga taaaaccctg
    24781 gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct gtttactatc
    24841 tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata acgtgaaggt
    24901 tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt gtattaaact
    24961 attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct tcgcttatat
    25021 taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg accaaaaagc
    25081 aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc atttacaata
    25141 taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac aggtaagcgg
    25201 taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga cttaagtggg
    25261 tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc caacatggca
    25321 tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat cgatcatgca
    25381 attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg gtgtggtgac
    25441 cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta tccgcgatat
    25501 cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt
    25561 tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg gtattgatat
    25621 cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc
    25681 ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct gttactggca
    25741 gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacac
    25801 caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat
    25861 tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta tctgatccct
    25921 gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa
    25981 ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct
    26041 attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa atattttgcc
    26101 ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat tattgcccgt
    26161 tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac
    26221 gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg
    26281 gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc
    26341 gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga tatgacaccg
    26401 cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac
    26461 ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa atacgggcag
    26521 gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gatgccttca
    26581 tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta
    26641 aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt tgctgacttg
    26701 cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg gacggaacga
    26761 atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga
    26821 tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca ggcttcctgg
    26881 cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac cagcgcactg
    26941 ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag acggggaata
    27001 aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt
    27061 ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct gcgggcaaac
    27121 tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg gtgataatcg
    27181 aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc gacagcccat
    27241 tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg tgtcccccag
    27301 ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat cactttagct
    27361 tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat tttttccagc
    27421 aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt ctggcataaa
    27481 aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac cttcatatca
    27541 atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc atgatgcaat
    27601 gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt tctgccagtc
    27661 tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc atcaataacc
    27721 agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg atagtacgat
    27781 agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct ttcatgccaa
    27841 ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta tccaccgcat
    27901 cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga ccgatcgcgc
    27961 ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc tctttaccgc
    28021 cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta acggtaaacc
    28081 ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt
    28141 agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta cgtaccgcgt
    28201 catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca tctgccagca
    28261 gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg gaagtaatca
    28321 cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc aaccagcggc
    28381 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
    28441 cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc accagatagt
    28501 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
    28561 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
    28621 cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga atgtccgccg
    28681 tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
    28741 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccagct
    28801 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
    28861 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
    28921 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgctagga
    28981 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
    29041 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
    29101 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
    29161 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
    29221 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
    29281 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
    29341 acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
    29401 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
    29461 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
    29521 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
    29581 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
    29641 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
    29701 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
    29761 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
    29821 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
    29881 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
    29941 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
    30001 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
    30061 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
    30121 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
    30181 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat
    30241 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
    30301 agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc gcacgcttcg
    30361 gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta acctccggcc
    30421 ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc
    30481 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
    30541 cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc ctgcggcatg
    30601 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc
    30661 agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat ggcgtcatcg
    30721 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
    30781 ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga
    30841 aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt tgatttgata
    30901 tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg attaatatca
    30961 atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt
    31021 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc gattaacccg
    31081 gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg tagggcatga
    31141 acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
    31201 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
    31261 aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg cgtggttgcc
    31321 ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga aacgccacgg
    31381 ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc cgggctggaa
    31441 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
    31501 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtta
    31561 ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc gcacgatccg
    31621 gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact
    31681 tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcgctaaga
    31741 ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt
    31801 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
    31861 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
    31921 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
    31981 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
    32041 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
    32101 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
    32161 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
    32221 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
    32281 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
    32341 acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag gcgaccagcg
    32401 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
    32461 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
    32521 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
    32581 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
    32641 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
    32701 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
    32761 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
    32821 cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
    32881 gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
    32941 ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
    33001 cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
    33061 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca atcgctttct
    33121 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct
    33181 ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga cctgcatcta
    33241 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
    33301 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
    33361 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
    33421 ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga ccaaagccca
    33481 cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
    33541 tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc
    33601 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
    33661 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
    33721 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
    33781 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
    33841 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct
    33901 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
    33961 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
    34021 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
    34081 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
    34141 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
    34201 ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca gccatcgggt
    34261 tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg
    34321 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
    34381 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
    34441 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
    34501 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg ttttctgccg
    34561 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
    34621 ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat ttttcatgca
    34681 actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc cagagaggta
    34741 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
    34801 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
    34861 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
    34921 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
    34981 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
    35041 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
    35101 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
    35161 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
    35221 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
    35281 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
    35341 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
    35401 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
    35461 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
    35521 aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag cttgttggcg
    35581 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
    35641 gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg
    35701 aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagagggg
//
LOCUS       DAJTRW010000033        34851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRW010000033 DAJTRW010000000
VERSION     DAJTRW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(18..93)
                     /locus_tag="OO326_001782"
     tRNA            complement(18..93)
                     /locus_tag="OO326_001782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO326_001783"
     tRNA            complement(152..228)
                     /locus_tag="OO326_001783"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="OO326_001784"
     CDS             complement(331..1716)
                     /locus_tag="OO326_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5966193.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO326_001785"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO326_001785"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5966194.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFECIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO326_001786"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO326_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5966195.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO326_001787"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO326_001787"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5966196.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO326_001788"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO326_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5966197.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO326_001789"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO326_001789"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5966198.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO326_001790"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO326_001790"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5966199.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO326_001791"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO326_001791"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5966200.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO326_001792"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO326_001792"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5966201.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO326_001793"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO326_001793"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5966202.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO326_001794"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO326_001794"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5966203.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO326_001795"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO326_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5966204.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO326_001796"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO326_001796"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5966205.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO326_001797"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO326_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5966206.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO326_001798"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO326_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5966207.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO326_001799"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO326_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5966208.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="OO326_001800"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="OO326_001800"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5966209.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="OO326_001801"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="OO326_001801"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5966210.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO326_001802"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO326_001802"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5966211.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="OO326_001803"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="OO326_001803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5966212.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO326_001804"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO326_001804"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5966213.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="OO326_001805"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="OO326_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5966214.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO326_001806"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO326_001806"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5966215.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO326_001807"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO326_001807"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5966216.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO326_001808"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO326_001808"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5966217.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO326_001809"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO326_001809"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5966218.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="OO326_001810"
     CDS             complement(29521..31167)
                     /locus_tag="OO326_001810"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5966219.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="OO326_001811"
     CDS             complement(31170..31220)
                     /locus_tag="OO326_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966220.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO326_001812"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO326_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5966221.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="OO326_001813"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="OO326_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO326_001814"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO326_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5966222.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="OO326_001815"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="OO326_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5966223.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(34652..34727)
                     /locus_tag="OO326_001816"
     tRNA            complement(34652..34727)
                     /locus_tag="OO326_001816"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
     gene            complement(34736..34812)
                     /locus_tag="OO326_001817"
     tRNA            complement(34736..34812)
                     /locus_tag="OO326_001817"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34776..34778),aa:Asp,seq:gtc)
BASE COUNT         8501 a         9537 c         8800 g         8013 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcattcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgatgtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    34741 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    34801 aactaccgct ccaccgaatt cttttacaac caccggtttt acgaccggct t
//
LOCUS       DAJTRW010000034        34778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRW010000034 DAJTRW010000000
VERSION     DAJTRW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..2098
                     /locus_tag="OO326_001818"
     CDS             <1..2098
                     /locus_tag="OO326_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5966224.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO326_001819"
     CDS             2095..3183
                     /locus_tag="OO326_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5966225.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO326_001820"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO326_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5966226.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO326_001821"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO326_001821"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5966227.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO326_001822"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO326_001822"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5966228.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO326_001823"
     CDS             8051..8602
                     /locus_tag="OO326_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5966229.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO326_001824"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO326_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5966230.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO326_001825"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO326_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5966231.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO326_001826"
     CDS             complement(11683..12681)
                     /locus_tag="OO326_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5966232.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO326_001827"
     CDS             complement(12760..13452)
                     /locus_tag="OO326_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5966233.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO326_001828"
     CDS             complement(13529..14032)
                     /locus_tag="OO326_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5966234.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO326_001829"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO326_001829"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5966235.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO326_001830"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO326_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5966236.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO326_001831"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO326_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5966237.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO326_001832"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO326_001832"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5966238.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO326_001833"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO326_001833"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5966239.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO326_001834"
     CDS             complement(20922..21341)
                     /locus_tag="OO326_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966240.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO326_001835"
     CDS             complement(21319..21672)
                     /locus_tag="OO326_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966241.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO326_001836"
     CDS             complement(21673..21993)
                     /locus_tag="OO326_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966242.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO326_001837"
     CDS             complement(22127..23560)
                     /locus_tag="OO326_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5966243.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO326_001838"
     CDS             complement(23623..24072)
                     /locus_tag="OO326_001838"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5966244.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO326_001839"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO326_001839"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5966245.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO326_001840"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO326_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5966246.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO326_001841"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO326_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO326_001842"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO326_001842"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5966247.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO326_001843"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO326_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO326_001844"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO326_001844"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5966248.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO326_001845"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO326_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO326_001846"
     tRNA            complement(34082..34157)
                     /locus_tag="OO326_001846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34778
                     /gene="mug"
                     /locus_tag="OO326_001847"
                     /pseudo
     CDS             34282..34778
                     /gene="mug"
                     /locus_tag="OO326_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8480 a         8944 c         9138 g         8216 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcg
//
LOCUS       DAJTRW010000035        34712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRW010000035 DAJTRW010000000
VERSION     DAJTRW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            667..2952
                     /gene="nrdA"
                     /locus_tag="OO326_001848"
     CDS             667..2952
                     /gene="nrdA"
                     /locus_tag="OO326_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5966249.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3186..4316
                     /gene="nrdB"
                     /locus_tag="OO326_001849"
     CDS             3186..4316
                     /gene="nrdB"
                     /locus_tag="OO326_001849"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5966250.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4316..4570
                     /gene="yfaE"
                     /locus_tag="OO326_001850"
     CDS             4316..4570
                     /gene="yfaE"
                     /locus_tag="OO326_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5966251.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4624..5274)
                     /gene="inaA"
                     /locus_tag="OO326_001851"
     CDS             complement(4624..5274)
                     /gene="inaA"
                     /locus_tag="OO326_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5966252.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5453..5695
                     /locus_tag="OO326_001852"
                     /pseudo
     CDS             5453..5695
                     /locus_tag="OO326_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5737..6813)
                     /gene="glpQ"
                     /locus_tag="OO326_001853"
     CDS             complement(5737..6813)
                     /gene="glpQ"
                     /locus_tag="OO326_001853"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5966253.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6818..8176)
                     /gene="glpT"
                     /locus_tag="OO326_001854"
     CDS             complement(6818..8176)
                     /gene="glpT"
                     /locus_tag="OO326_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5966254.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8449..10077
                     /gene="glpA"
                     /locus_tag="OO326_001855"
     CDS             8449..10077
                     /gene="glpA"
                     /locus_tag="OO326_001855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5966255.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10067..11326
                     /gene="glpB"
                     /locus_tag="OO326_001856"
     CDS             10067..11326
                     /gene="glpB"
                     /locus_tag="OO326_001856"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5966256.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11323..12513
                     /gene="glpC"
                     /locus_tag="OO326_001857"
     CDS             11323..12513
                     /gene="glpC"
                     /locus_tag="OO326_001857"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5966257.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12706..13656
                     /locus_tag="OO326_001858"
     CDS             12706..13656
                     /locus_tag="OO326_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5966258.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13697..14167)
                     /locus_tag="OO326_001859"
     CDS             complement(13697..14167)
                     /locus_tag="OO326_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5966259.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14222..15424)
                     /locus_tag="OO326_001860"
     CDS             complement(14222..15424)
                     /locus_tag="OO326_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5966260.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15511..16053)
                     /locus_tag="OO326_001861"
     CDS             complement(15511..16053)
                     /locus_tag="OO326_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966261.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16332..16757
                     /gene="nudI"
                     /locus_tag="OO326_001862"
     CDS             16332..16757
                     /gene="nudI"
                     /locus_tag="OO326_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5966262.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16796..17398)
                     /locus_tag="OO326_001863"
     CDS             complement(16796..17398)
                     /locus_tag="OO326_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5966263.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17706..18845
                     /gene="arnB"
                     /locus_tag="OO326_001864"
     CDS             17706..18845
                     /gene="arnB"
                     /locus_tag="OO326_001864"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5966264.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18849..19817
                     /gene="arnC"
                     /locus_tag="OO326_001865"
     CDS             18849..19817
                     /gene="arnC"
                     /locus_tag="OO326_001865"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5966265.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19817..21798
                     /gene="arnA"
                     /locus_tag="OO326_001866"
                     /pseudo
     CDS             19817..21798
                     /gene="arnA"
                     /locus_tag="OO326_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21795..22685
                     /locus_tag="OO326_001867"
     CDS             21795..22685
                     /locus_tag="OO326_001867"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5966266.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22685..24337
                     /gene="arnT"
                     /locus_tag="OO326_001868"
     CDS             22685..24337
                     /gene="arnT"
                     /locus_tag="OO326_001868"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5966267.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATAIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24334..24669
                     /gene="arnE"
                     /locus_tag="OO326_001869"
     CDS             24334..24669
                     /gene="arnE"
                     /locus_tag="OO326_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5966268.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24669..25055
                     /gene="arnF"
                     /locus_tag="OO326_001870"
     CDS             24669..25055
                     /gene="arnF"
                     /locus_tag="OO326_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5966269.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25049..25321)
                     /gene="pmrD"
                     /locus_tag="OO326_001871"
     CDS             complement(25049..25321)
                     /gene="pmrD"
                     /locus_tag="OO326_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5966270.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25426..26781)
                     /gene="menE"
                     /locus_tag="OO326_001872"
     CDS             complement(25426..26781)
                     /gene="menE"
                     /locus_tag="OO326_001872"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5966271.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26778..27740)
                     /gene="menC"
                     /locus_tag="OO326_001873"
     CDS             complement(26778..27740)
                     /gene="menC"
                     /locus_tag="OO326_001873"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5966272.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27740..28597)
                     /gene="menB"
                     /locus_tag="OO326_001874"
     CDS             complement(27740..28597)
                     /gene="menB"
                     /locus_tag="OO326_001874"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5966273.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28612..29370)
                     /gene="menH"
                     /locus_tag="OO326_001875"
     CDS             complement(28612..29370)
                     /gene="menH"
                     /locus_tag="OO326_001875"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5966274.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29367..31037)
                     /gene="menD"
                     /locus_tag="OO326_001876"
     CDS             complement(29367..31037)
                     /gene="menD"
                     /locus_tag="OO326_001876"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5966275.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31126..32421)
                     /gene="menF"
                     /locus_tag="OO326_001877"
     CDS             complement(31126..32421)
                     /gene="menF"
                     /locus_tag="OO326_001877"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5966276.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32500..32805)
                     /gene="elaB"
                     /locus_tag="OO326_001878"
     CDS             complement(32500..32805)
                     /gene="elaB"
                     /locus_tag="OO326_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5966277.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32860..33321)
                     /locus_tag="OO326_001879"
     CDS             complement(32860..33321)
                     /locus_tag="OO326_001879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5966278.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33386..34303
                     /gene="rbn"
                     /locus_tag="OO326_001880"
     CDS             33386..34303
                     /gene="rbn"
                     /locus_tag="OO326_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5966279.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34494..34682
                     /locus_tag="OO326_001881"
                     /pseudo
     CDS             34494..34682
                     /locus_tag="OO326_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8477 a         9298 c         8607 g         8330 t
ORIGIN      
        1 ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt gtccaacccg
       61 atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata attcaagtta
      121 ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat aattttctta
      181 taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat agaaaacaac
      241 catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact gatacatcag
      301 attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa gattaataaa
      361 ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga acagttattt
      421 ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc taaggtgcgc
      481 gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa gagggtcatt
      541 ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt ctaagcagct
      601 tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa aacaggtacg
      661 acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga gcgcatcaat
      721 ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa cgtttcgatt
      781 tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac ctctgacatc
      841 cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc ggattatcag
      901 tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg ccagtttgag
      961 ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata cgataatcat
     1021 ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat cgatcacgac
     1081 cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata tctagtacag
     1141 aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct tgttgccgcg
     1201 tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg tttttatgac
     1261 gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt gcgtaccccg
     1321 actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga ttccatcaac
     1381 gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg catcaacgcc
     1441 ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca taccggctgt
     1501 atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg cggtgtgcgc
     1561 ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag cctgctggtg
     1621 ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta cggggtacaa
     1681 atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct gttcagcccg
     1741 tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt tgaacgcctg
     1801 tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc cgttgagctg
     1861 ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca gaacgttgac
     1921 cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca gtctaacctg
     1981 tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga gaacggtgaa
     2041 atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct ggatgaactg
     2101 gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta tcaggattac
     2161 ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat tggtgtgatt
     2221 aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag cgccaacaac
     2281 ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc taatgagctg
     2341 gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa agggatcctg
     2401 ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct gcattacgac
     2461 tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac gctttctgcc
     2521 ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat tgaaccgccg
     2581 cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt ggtgccggac
     2641 tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa cgatggctac
     2701 ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc taacaccaac
     2761 tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct gaaagacctg
     2821 ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg tgacggcgct
     2881 gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga aagcggcgca
     2941 tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg cctacggctc
     3001 ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc cggatgcagc
     3061 gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg cgccagcgtc
     3121 gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc caacaggaca
     3181 cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa gaaccgatgt
     3241 tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac atcttcgaaa
     3301 agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac gtctcccgcg
     3361 accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc agcaacctga
     3421 aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg ctattgccgc
     3481 ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca gaaacgattc
     3541 attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct gttgtgtttg
     3601 acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc agctattacg
     3661 atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc cacaccgtta
     3721 acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat ctctgcctga
     3781 tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt tcctttgcct
     3841 ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt gcccgcgatg
     3901 aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc ggcgcggatg
     3961 atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac ctgtttgtcc
     4021 aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt tcgatgattg
     4081 gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc cgcatgcagg
     4141 cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg atcaacacct
     4201 ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt tcttatctgg
     4261 tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag ctctgatggc
     4321 ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac acccttccct
     4381 tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg aaggttactg
     4441 cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg aaccgttagc
     4501 ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg atattgaaat
     4561 cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc tggcctacaa
     4621 cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag ggatcggctc
     4681 cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac gcaagcggcg
     4741 acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt tcacatagat
     4801 atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct taaacgccag
     4861 tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata ctgcatgccg
     4921 ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca gcaacgcgcg
     4981 ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga tgacaccggc
     5041 ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc cgaacggata
     5101 acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt tgccgttgcg
     5161 ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt caacccagtc
     5221 gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg ccatacccta
     5281 tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga aaatcttaat
     5341 gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg cttttgccct
     5401 tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc agcagataaa
     5461 atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat taggcatcgc
     5521 cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg ttcctcacga
     5581 accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag aaggcagtcc
     5641 actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa tttgatatga
     5701 gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa gaaattttac
     5761 tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat acagagcatc
     5821 atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca cggtataagg
     5881 atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt tgatattacc
     5941 cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat ctgcatattc
     6001 cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat taacccagct
     6061 tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca gctgtaccag
     6121 attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca gctcatcagc
     6181 atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt tcttcagcac
     6241 ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg gcgctttgat
     6301 ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa caaattcaat
     6361 ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac gccccggata
     6421 agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca gcgacttaat
     6481 ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc gatccgggaa
     6541 acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa ccagatggtc
     6601 gtctttggtc atcaccaaat cctgttccag ataatctgct ccctgcgcat acgccatcgc
     6661 ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg cgatgactat
     6721 tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc tcatcatgat
     6781 cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg cgtttttgca
     6841 gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag ataaccgcca
     6901 gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag tccacggtgt
     6961 agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc ccggtaaagc
     7021 ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga ccgatcagca
     7081 tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg acggttgggt
     7141 tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc ataaagaaga
     7201 cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg cacagcaggg
     7261 tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta tccagcgcga
     7321 aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa cgcagcagat
     7381 aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc acgtactgca
     7441 tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg tccggataat
     7501 catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg cgcatcatcg
     7561 caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc gccgcatgcc
     7621 agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca ccaccgacgt
     7681 tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac caccagtgca
     7741 ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag aggaacaaca
     7801 gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata aacaacatca
     7861 ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag cgatccgata
     7921 ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa agggcaaaac
     7981 ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc gcaaagttct
     8041 tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt tgccagcgca
     8101 atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt ggcgctggtt
     8161 taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga ttaacagcct
     8221 gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa acattcataa
     8281 attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt caaaatgacg
     8341 catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca aacaattcat
     8401 gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat gaaaactcgc
     8461 gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc cgggattgcc
     8521 cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga catcgcaacc
     8581 ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc agtaaccgat
     8641 gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat tgcacgtcac
     8701 tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct ctcattccag
     8761 gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat agacccgcag
     8821 caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt gaaagttccg
     8881 gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc caaagaacac
     8941 ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg cgcgacggtg
     9001 tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca tgcacctgtc
     9061 gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga tctgcgcatt
     9121 cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa ccagcatgtg
     9181 atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga taccatttcg
     9241 ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa tcgagtgacg
     9301 gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt gatggcgaaa
     9361 acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga tgacgacccg
     9421 agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg cgatggtctg
     9481 gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat ggctgaatgg
     9541 gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac tgccgatctg
     9601 gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat ctccctgcct
     9661 gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc ctggctgagc
     9721 gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc gggtgaagtg
     9781 cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg tcgtacccgt
     9841 gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg actgctgcaa
     9901 cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt ccttaacgaa
     9961 cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag cgaatttacc
    10021 cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc gctttgatac
    10081 tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc aaaaacacgg
    10141 cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat ccggttcgct
    10201 ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca gtggactgga
    10261 atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac aacgcgtgct
    10321 cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat tgcagggcag
    10381 cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa cctggttaag
    10441 ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag tggggataag
    10501 cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac tggatctttc
    10561 ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata acgccaccga
    10621 atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc cgctgttact
    10681 tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg cctgcttcgg
    10741 cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt cactgatgct
    10801 tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt tacagcgcca
    10861 gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag tgacctgtaa
    10921 aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc tacgtccacg
    10981 tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg aacgtaacgg
    11041 cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg gtgaatggta
    11101 taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca ctgatgagac
    11161 gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg gttcggtgct
    11221 gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg tcagtgcttt
    11281 acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga caccagcttc
    11341 gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg ggtgaatccc
    11401 ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt gaaagatggc
    11461 gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga agtcgcctgc
    11521 ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata tgacaccacg
    11581 cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag cgtttccacg
    11641 ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca gctgcttgat
    11701 gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg cacgttccgt
    11761 cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt cgctttcttt
    11821 cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat taaagtgctc
    11881 aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg cgtaccgcta
    11941 atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt agagtcgatc
    12001 cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac ctgtacattt
    12061 gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt gcgcgatcat
    12121 atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac gttaccgctg
    12181 aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa aatgggctgg
    12241 acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac ggtgctggat
    12301 tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta ccccacctca
    12361 caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga tctggtggtc
    12421 accgactgcg aaacctgtaa atggcagatt gagatgtcca caagtcttcg ctgcgaacat
    12481 ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc ggtaagcatg
    12541 tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt gcaacgcagt
    12601 gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa aggcctggga
    12661 taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac cgaatcaaca
    12721 acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga aaccgcacgg
    12781 gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct gcaaacctta
    12841 cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc ggatgttttg
    12901 tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga acatcaaagc
    12961 tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc catgcagagt
    13021 cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt ttatcatggc
    13081 gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga tccgcaactt
    13141 gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt acctgacgat
    13201 gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca tattcgcgac
    13261 cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg cttcactaat
    13321 gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac ctcccgtttc
    13381 acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag attaatgact
    13441 attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg taaattagta
    13501 ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca agccattaaa
    13561 attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc ggccacccag
    13621 ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca cagccgatcc
    13681 ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg cctgatttaa
    13741 acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga
    13801 caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca gccagaaaac
    13861 cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt cgctgcactt
    13921 ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct ggtccaataa
    13981 acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt gccgttttac
    14041 tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa ttctcaatgc
    14101 gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg tagggagggt
    14161 tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca gagtggcgaa
    14221 atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca ctagcgatgt
    14281 cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac acttcctgac
    14341 ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa gtgccgtctg
    14401 gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc agtgccagcc
    14461 cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc agggtttctt
    14521 cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa agttgcaaag
    14581 ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg ttttgcaatt
    14641 cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc tgtccggcaa
    14701 cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc ggtccggtga
    14761 gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc ggcggcagtt
    14821 gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta gttaaacgca
    14881 gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg atttcgtgct
    14941 cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg caacgattaa
    15001 gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca gcactggcag
    15061 gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg tggaaatagc
    15121 gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt gctgtcgcag
    15181 cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg atcagcacat
    15241 cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta tcccccaccg
    15301 tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc caggcagcgt
    15361 tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt tccactttta
    15421 acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag ataaggcgta
    15481 gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc caacatacag
    15541 gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca ggtagcgata
    15601 acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat tcgcttcctc
    15661 ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc caaacaaacg
    15721 gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat aaccttcatc
    15781 gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta acggcccgag
    15841 aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt tatgtgtcca
    15901 gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa tattggtgta
    15961 ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa ccaacagcat
    16021 tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa gcaattaatc
    16081 aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg agcgaaaaaa
    16141 gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc gaaaaaataa
    16201 acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg caaaagagat
    16261 tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt ttatttataa
    16321 ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat gatggtgctt
    16381 atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg gcgctttcgg
    16441 gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa attcgcgaag
    16501 aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc gatgatattc
    16561 gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac ctgatttttg
    16621 actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac tacgcgtggg
    16681 taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa acgttacgtt
    16741 tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg aaaatttagt
    16801 ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg actaaaccat
    16861 ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat gtcaggcaat
    16921 gattatgggt gaaaataacg atatttttat caggcgcttt gctttgtaag tccttaattg
    16981 cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc tttttacccg
    17041 cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa agatcgaaat
    17101 cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta cctttaaccg
    17161 taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt tcagcatggc
    17221 gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta ttgtcgatac
    17281 gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt gcagcgagcg
    17341 ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa gctaacacgt
    17401 aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc gacatttctg
    17461 gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa atgcctcaaa
    17521 tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata tttacgtcta
    17581 atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc caaatggcaa
    17641 aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg gcggaaggaa
    17701 aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag gaactcgctg
    17761 cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat caggcgcttg
    17821 agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt tcagccaccg
    17881 ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa gtgattacgc
    17941 cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca acgccggtaa
    18001 tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa tcagccatta
    18061 cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca gatattgacg
    18121 ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct gcccatgccg
    18181 tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt ttttcatttc
    18241 atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat aatgaaaacc
    18301 ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc tatgacagac
    18361 aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag tacaatctga
    18421 ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac ctcaacaccc
    18481 gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc tttcagccat
    18541 taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt cgtgtcgatg
    18601 aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa agaggcattg
    18661 gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag cgttttccca
    18721 cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg ccgctgttcc
    18781 cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa ctcgcaggac
    18841 aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt cccgtttata
    18901 acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt gaatcgttgg
    18961 ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc gcgcatatgc
    19021 tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt aaccgcaatt
    19081 acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac ttaattatta
    19141 cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg gcaaaagccg
    19201 atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc tggtttcgta
    19261 aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa gcgatgggtg
    19321 actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc
    19381 atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt gcaattgaaa
    19441 ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt atgcgcctga
    19501 ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt atgctgagtc
    19561 tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg gtgattttac
    19621 gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt gccgtgctgt
    19681 ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat atcggcagga
    19741 tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc cgtccatcca
    19801 gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg
    19861 tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc ataccgataa
    19921 tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa gagacattcc
    19981 ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg cccaactgtc
    20041 accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga tttttcagct
    20101 cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc gtggtcgcgc
    20161 gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat tgcaccgaat
    20221 ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat tgcgccagac
    20281 gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag
    20341 acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc
    20401 acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa accggcatcc
    20461 gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt
    20521 ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag
    20581 ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa
    20641 atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagatg
    20701 ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc ccgacgccgt
    20761 acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg
    20821 ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt
    20881 tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca ttccgaatgg
    20941 attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat agccacgccg
    21001 attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga gaatctgcgc
    21061 attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt
    21121 tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat cgtcggcccg
    21181 gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg ggtgatctgg
    21241 gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa ctggatggga
    21301 ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat tacgcaactc
    21361 attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa acaaaaacgc
    21421 tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga aaatgcggga
    21481 aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgag
    21541 gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca
    21601 ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg atatcaggac
    21661 gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg ggagccgaaa
    21721 attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt
    21781 acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc tttcgtggca
    21841 cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca
    21901 tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac
    21961 agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac
    22021 tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg
    22081 aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc tggcaagccc
    22141 gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt cttcgcactc
    22201 tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc
    22261 agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg
    22321 ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa attccggtga
    22381 ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt
    22441 ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt catgcagaag
    22501 ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca gctcaggaag
    22561 gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc ggacaagttg
    22621 ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc
    22681 gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct gctattacct
    22741 gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg cggaaatcag
    22801 tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc tacgttattt
    22861 cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa
    22921 taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac
    22981 ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca cagcaattta
    23041 tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc
    23101 attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa
    23161 aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa
    23221 aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa
    23281 acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt
    23341 tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg
    23401 ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc cgttctggta
    23461 ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact
    23521 gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat
    23581 aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata ttctttcttg
    23641 ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa aaaataatcc
    23701 tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc
    23761 cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag
    23821 ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac
    23881 cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg ggctggcgtt
    23941 gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt
    24001 cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt
    24061 tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg
    24121 tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga
    24181 gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt
    24241 tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca tcgttcgtca
    24301 ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc
    24361 agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata
    24421 aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt
    24481 gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg
    24541 ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta
    24601 tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt
    24661 acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat
    24721 taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg
    24781 cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc
    24841 tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg
    24901 ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg
    24961 aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc
    25021 tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc
    25081 gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt
    25141 cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg
    25201 cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa
    25261 gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca ttccattgca
    25321 ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa actctttatc
    25381 aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt gctgacgttg
    25441 cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca gctccggcgg
    25501 taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca cccattcgcc
    25561 aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac caaactcctt
    25621 gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa taacacgctc
    25681 gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta aacgtccgac
    25741 aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc agccttcgtc
    25801 attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac tggcagcccg
    25861 cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca aaccaacgtc
    25921 tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca gaccatagcc
    25981 gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc
    26041 gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca aacgccagag
    26101 ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca atggctgttt
    26161 atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc cctgaccgga
    26221 gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg gaatcagcga
    26281 taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg ctttcggcaa
    26341 accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc acgcagcggc
    26401 atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg tgttttcccc
    26461 atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa gcaacggttg
    26521 cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg ccagccaggc
    26581 gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct caaccacccc
    26641 ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc gccagttgag
    26701 ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca
    26761 cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac taacggcagt
    26821 gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt gtccagtccc
    26881 ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag acctaagctc
    26941 gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc ctgtacctgc
    27001 tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc gcgcacgccc
    27061 tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc aatggcaatg
    27121 ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaac
    27181 gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc tttcagcggt
    27241 gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc cagcaacaga
    27301 ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt cgccactttc
    27361 tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt acacagcggt
    27421 gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa tgcacagctt
    27481 acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag ccagttattt
    27541 acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa gcccggcagt
    27601 ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta cagcccgtcg
    27661 cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg
    27721 tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca ggctgacgtt
    27781 tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac agcatggtgg
    27841 cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc agcgcagctt
    27901 tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa cggacggttt
    27961 ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc agcgcctgtt
    28021 ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc ccgacgatac
    28081 gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac
    28141 cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga acgtgaccac
    28201 cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac
    28261 gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat
    28321 caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa
    28381 tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc atctctttta
    28441 ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac
    28501 cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg tgccattcaa
    28561 ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta gtcagaaacg
    28621 caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg cgttatgtcc
    28681 ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc ggaatttgct
    28741 gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa
    28801 atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg cgccattatt
    28861 gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg
    28921 ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac gctgcgccca
    28981 ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc ccggatgccc
    29041 gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca tcatcgccac
    29101 ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac taaccaaggt
    29161 tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc
    29221 atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc ccacttcttg
    29281 ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta aacctagttt
    29341 tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag aagttgctgg
    29401 agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt ggttggcgtg
    29461 cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg atgatatttc
    29521 agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc
    29581 tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt gttgttcacc
    29641 acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt gagatcgtaa
    29701 agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg ctgaacgcct
    29761 gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta caccgggtaa
    29821 cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt accaacaaac
    29881 aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac
    29941 gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg gatttcaacg
    30001 caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat attggcaatt
    30061 aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc aacaatccag
    30121 tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt caggctactt
    30181 cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc attgcctaac
    30241 caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc aatcagcggc
    30301 cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact catgcgcccg
    30361 gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt ttcactttcc
    30421 agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc cagacgctgt
    30481 tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg
    30541 atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa
    30601 cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg gtgagaggcg
    30661 aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc cggcgggcga
    30721 tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag tgccggatag
    30781 agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt gctgactttc
    30841 gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg gtgaatgaat
    30901 gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg ggcgatacag
    30961 atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta
    31021 aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta
    31081 caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa
    31141 taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga
    31201 accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa tttttgctga
    31261 gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag aaccggcgta
    31321 ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa
    31381 gccagcaact gctgccgttg gcagcaactg atgtaaacag atcacatcat ccgctttgtt
    31441 gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg
    31501 cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat cttccaccac
    31561 cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta actgctgcgc
    31621 ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac gcagcgcttt
    31681 gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt cgccatcaaa
    31741 ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca tcatcgccgc
    31801 cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca ctttgtcgag
    31861 ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc
    31921 cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct tgatactcac
    31981 cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc tttcgctgaa
    32041 tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc ggggtaaaag
    32101 taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt cagcgtgttc
    32161 cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc
    32221 caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg ggtacgtttg
    32281 ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcgat
    32341 aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag
    32401 cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg
    32461 tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag
    32521 tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt tttcgtggac
    32581 gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg
    32641 gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca gctcaacata
    32701 tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt cactaagcag
    32761 cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg ttctccattt
    32821 gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa ttacctcgcg
    32881 tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag ggataaaacc
    32941 gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag gcttatcagg
    33001 ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca ctttttcacc
    33061 acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat catcatcact
    33121 tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa tatggcgatt
    33181 atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct cgacaacaaa
    33241 caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt cagagtgatg
    33301 cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat actagccgga
    33361 atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt cagccggtgt
    33421 gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag
    33481 cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt
    33541 taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc atctttttgg
    33601 tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct taacgattta
    33661 tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg gctcatggac
    33721 cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg atggcctgcg
    33781 caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc gtattgaaga
    33841 acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg tgccgcctgg
    33901 cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg gaaggcagat
    33961 taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta ttttcggcga
    34021 taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca tggtgcacga
    34081 agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata gctctacacg
    34141 ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta cccacgtcag
    34201 ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt caattttccc
    34261 ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct tattattaaa
    34321 aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac ccacacaggc
    34381 atattgagcg atttttgata tttattcatc tccttacgca ctatattcag agcaataagt
    34441 tgcctctttt catcttgatt cagcacaacc tttgcaacta aaaggagccc atgatggtta
    34501 cagttgtcag caattattgt caattatctc aaacccaact cagtcagaca tttgcagaaa
    34561 aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta tccggagatg
    34621 aagaaagtat tgagttactg cataatattg cgttaggtta tgatgaattt ggaaaaaaag
    34681 ctgggtaatg ctgccaactt actgatttag tg
//
LOCUS       DAJTRW010000036        34447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRW010000036 DAJTRW010000000
VERSION     DAJTRW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..334)
                     /locus_tag="OO326_001882"
     CDS             complement(<1..334)
                     /locus_tag="OO326_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966280.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO326_001883"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO326_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5966281.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO326_001884"
     CDS             complement(1695..2075)
                     /locus_tag="OO326_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5966282.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO326_001885"
     CDS             complement(2089..2505)
                     /locus_tag="OO326_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966283.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO326_001886"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO326_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5966284.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO326_001887"
     CDS             complement(3506..3973)
                     /locus_tag="OO326_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5966285.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO326_001888"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO326_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5966286.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO326_001889"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO326_001889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5966287.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO326_001890"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO326_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5966288.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO326_001891"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO326_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5966289.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO326_001892"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO326_001892"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5966290.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO326_001893"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO326_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5966291.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO326_001894"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO326_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5966292.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO326_001895"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO326_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5966293.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO326_001896"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO326_001896"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5966294.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO326_001897"
     CDS             complement(14628..15443)
                     /locus_tag="OO326_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5966295.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO326_001898"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO326_001898"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5966296.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO326_001899"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO326_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5966297.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO326_001900"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO326_001900"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5966298.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO326_001901"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO326_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5966299.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO326_001902"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO326_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5966300.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO326_001903"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO326_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5966301.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO326_001904"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO326_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5966302.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="OO326_001905"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="OO326_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5966303.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="OO326_001906"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="OO326_001906"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5966304.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO326_001907"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO326_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5966305.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="OO326_001908"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="OO326_001908"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5966306.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="OO326_001909"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="OO326_001909"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5966307.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO326_001910"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO326_001910"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5966308.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO326_001911"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO326_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5966309.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="OO326_001912"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="OO326_001912"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5966310.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="OO326_001913"
     CDS             31577..32191
                     /locus_tag="OO326_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5966311.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="OO326_001914"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="OO326_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5966312.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="OO326_001915"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="OO326_001915"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5966313.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8699 c         9302 g         8338 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagtga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgctctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg taacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgataa tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgt atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt aatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc agccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctttgtagcg ggaaagcgta ttatgaacac
    34441 cccgcgc
//
LOCUS       DAJTRW010000037        34352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRW010000037 DAJTRW010000000
VERSION     DAJTRW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..168)
                     /locus_tag="OO326_001916"
     CDS             complement(<1..168)
                     /locus_tag="OO326_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966314.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLL"
     gene            260..346
                     /locus_tag="OO326_001917"
                     /pseudo
     CDS             260..346
                     /locus_tag="OO326_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(751..1227)
                     /locus_tag="OO326_001918"
     CDS             complement(751..1227)
                     /locus_tag="OO326_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5966315.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1286..2575)
                     /gene="bioA"
                     /locus_tag="OO326_001919"
     CDS             complement(1286..2575)
                     /gene="bioA"
                     /locus_tag="OO326_001919"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5966316.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2662..3702
                     /gene="bioB"
                     /locus_tag="OO326_001920"
     CDS             2662..3702
                     /gene="bioB"
                     /locus_tag="OO326_001920"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5966317.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3699..4853
                     /gene="bioF"
                     /locus_tag="OO326_001921"
     CDS             3699..4853
                     /gene="bioF"
                     /locus_tag="OO326_001921"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5966318.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4840..5595
                     /gene="bioC"
                     /locus_tag="OO326_001922"
     CDS             4840..5595
                     /gene="bioC"
                     /locus_tag="OO326_001922"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5966319.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5588..6265
                     /gene="bioD"
                     /locus_tag="OO326_001923"
     CDS             5588..6265
                     /gene="bioD"
                     /locus_tag="OO326_001923"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5966320.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6844..8865
                     /gene="uvrB"
                     /locus_tag="OO326_001924"
     CDS             6844..8865
                     /gene="uvrB"
                     /locus_tag="OO326_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5966321.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9057..9965)
                     /gene="yvcK"
                     /locus_tag="OO326_001925"
     CDS             complement(9057..9965)
                     /gene="yvcK"
                     /locus_tag="OO326_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5966322.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10230..10374
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10362..11351
                     /gene="moaA"
                     /locus_tag="OO326_001926"
     CDS             10362..11351
                     /gene="moaA"
                     /locus_tag="OO326_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5966323.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11373..11885
                     /gene="moaB"
                     /locus_tag="OO326_001927"
     CDS             11373..11885
                     /gene="moaB"
                     /locus_tag="OO326_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5966324.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11888..12373
                     /gene="moaC"
                     /locus_tag="OO326_001928"
     CDS             11888..12373
                     /gene="moaC"
                     /locus_tag="OO326_001928"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5966325.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12366..12611
                     /gene="moaD"
                     /locus_tag="OO326_001929"
     CDS             12366..12611
                     /gene="moaD"
                     /locus_tag="OO326_001929"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5966326.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12613..13065
                     /gene="moaE"
                     /locus_tag="OO326_001930"
     CDS             12613..13065
                     /gene="moaE"
                     /locus_tag="OO326_001930"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5966327.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13202..13906
                     /locus_tag="OO326_001931"
     CDS             13202..13906
                     /locus_tag="OO326_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5966328.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14111..14824
                     /locus_tag="OO326_001932"
     CDS             14111..14824
                     /locus_tag="OO326_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5966329.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(14860..15816)
                     /locus_tag="OO326_001933"
     CDS             complement(14860..15816)
                     /locus_tag="OO326_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5966330.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(15816..17057)
                     /gene="clsB"
                     /locus_tag="OO326_001934"
     CDS             complement(15816..17057)
                     /gene="clsB"
                     /locus_tag="OO326_001934"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5966331.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17054..17815)
                     /locus_tag="OO326_001935"
     CDS             complement(17054..17815)
                     /locus_tag="OO326_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5966332.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17948..18358
                     /locus_tag="OO326_001936"
     CDS             17948..18358
                     /locus_tag="OO326_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5966333.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18320..19426)
                     /locus_tag="OO326_001937"
     CDS             complement(18320..19426)
                     /locus_tag="OO326_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5966334.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19437..20570)
                     /locus_tag="OO326_001938"
     CDS             complement(19437..20570)
                     /locus_tag="OO326_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5966335.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20563..22299)
                     /locus_tag="OO326_001939"
     CDS             complement(20563..22299)
                     /locus_tag="OO326_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5966336.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22292..23287)
                     /gene="hlyD"
                     /locus_tag="OO326_001940"
     CDS             complement(22292..23287)
                     /gene="hlyD"
                     /locus_tag="OO326_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5966337.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23290..23961)
                     /gene="cecR"
                     /locus_tag="OO326_001941"
     CDS             complement(23290..23961)
                     /gene="cecR"
                     /locus_tag="OO326_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5966338.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24190..25377
                     /gene="rhlE"
                     /locus_tag="OO326_001942"
                     /pseudo
     CDS             24190..25377
                     /gene="rhlE"
                     /locus_tag="OO326_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(25584..26066)
                     /gene="ybiA"
                     /locus_tag="OO326_001943"
     CDS             complement(25584..26066)
                     /gene="ybiA"
                     /locus_tag="OO326_001943"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5966339.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26186..28336
                     /gene="dinG"
                     /locus_tag="OO326_001944"
     CDS             26186..28336
                     /gene="dinG"
                     /locus_tag="OO326_001944"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5966340.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28364..29326
                     /gene="ybiB"
                     /locus_tag="OO326_001945"
     CDS             28364..29326
                     /gene="ybiB"
                     /locus_tag="OO326_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5966341.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29467..30552
                     /gene="hcxB"
                     /locus_tag="OO326_001946"
     CDS             29467..30552
                     /gene="hcxB"
                     /locus_tag="OO326_001946"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5966342.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(30781..31041)
                     /locus_tag="OO326_001947"
     CDS             complement(30781..31041)
                     /locus_tag="OO326_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5966343.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31306..31572)
                     /gene="ybiI"
                     /locus_tag="OO326_001948"
     CDS             complement(31306..31572)
                     /gene="ybiI"
                     /locus_tag="OO326_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5966344.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(31646..32323)
                     /gene="ybiX"
                     /locus_tag="OO326_001949"
     CDS             complement(31646..32323)
                     /gene="ybiX"
                     /locus_tag="OO326_001949"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5966345.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32365..34352)
                     /locus_tag="OO326_001950"
                     /pseudo
     CDS             complement(32365..34352)
                     /locus_tag="OO326_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8117 a         8874 c         9262 g         8099 t
ORIGIN      
        1 cagtaatcga actgcctgtg ttcggtcttc tgttgcagca ccttccttgg cccagttatc
       61 ccagatgcgg tgataatccg tgatattttg ctcttctacg gataaacggg atatacatcc
      121 taaaccacaa aaggagcgga aaaaagaagg attgtgggca ggtttcattt attattgatc
      181 ctcattgttt atatattaat atattcgtgc aaatggagtg aggattcttt aaaaaaacgc
      241 gcctgtaaat agttaagaac aggaagaact gttggggatg tggctggccg aaaatgaagg
      301 ggtgaagttc tggctcaatg tgctgactga actgaaaaaa cgcggttggt cgccctacct
      361 tttcatcaag ccagtagtct gcccaccaca gcatcatttc tctgcgctta tcgagatact
      421 gagcatggtt gtaaattcag ctaacccagt gcgttgacaa aaaattagcg caagaggaca
      481 aaaaatcacc ttgcgctaat gctctgtatc aggtcactaa taccatctaa gtggttgatt
      541 catagtgact ggatatgttg tgttttgcag tattatgcag tctatttttt aagctaaatg
      601 tattataatg cattgatatt aatgattttt actgtttctc ggtcagcttt tttatactaa
      661 cttgagcgaa acgggaaggt aaaaagacaa aaagttgttt ttaatacctt taagtgatac
      721 cagatggcat tgcgccatct ggcagagtga ttaactaaac atcgcggtaa tcgatgcgct
      781 tgccagagag tggaaatgaa cgttaaaccc gaccatcgcg ccgctggctc cttcatcgac
      841 atcaatacgc tctacatcca gcgcgtgaac ggtaaaaatg tagcgatggg tttcgccttt
      901 cggcggcgct gcgccatcgt atccggtttt accaaaatcg gtacgcgtct gcaaaacgcc
      961 gtctggcatt gctaccagac cagagccaaa cccttgcggt aatacgcggg tatcagcggg
     1021 taaattaaca actacccagt gccaccagcc ggagccggtt ggcgcatccg ggtcgtagca
     1081 ggtgacaaca aaacttttcg ttcccgcagg aacatcatcc cacgccagat gcggtgaaat
     1141 attatcgcca tcgtaaccca tgccgttaaa gacatgacga tgcggcaact tatcgccatc
     1201 gcgcagatcg ttactgatga gtttcatgaa ccctcctttc ttgtttgcag aaagtgtagc
     1261 cagaaaccct cacgctgact tcccgttatt ggcaaaaaaa tgtttcatcc tgtaccgcgc
     1321 ggttaaccgc tgcggtcaga cgctgcaact gttgcgggag aataatatag ggcggcatca
     1381 ggtaaatcag tttgccaaaa ggccggatcc ataccccctg ttcgacaaag aatttttgca
     1441 gcgccgccat attcaccgga cgagtggttt cgaccacacc gattgccccc agtacgcgca
     1501 catcggcaac catttcggca tcacgggctg gtgccagttg ctcgcgcagc tgcacttcaa
     1561 tagccgccac ctgctgctgc cattcgccgg attcgataat cgctaggctg gcgtttgctg
     1621 ccgcgcaggc cagcggattg cccataaaag ttggcccatg cataaagcag ccggcttcgc
     1681 cgttactgat ggtttctgca acctcgcgcg tggtaagtgt ggcggaaagg gtcattgtgc
     1741 cgccggttaa ggctttaccg aggcacaaaa tgtccggcgc gatttctgca tattcacagg
     1801 caaacagttt gccggtacga ccaaatccgg tggcgatctc gtcggcaatc agcaagatac
     1861 cttcgcgatc gcacattttg cggattcgtt ttaaccattc cggatggtac atgcgcatcc
     1921 cgcctgcgcc ctggacaatc ggctcaatga tcaccgctgc gatttcatga cgatgcgccg
     1981 ccatcaggcg ggcaaaacca accatatcgc gttcatccca ttcaccatcc atgcggcttt
     2041 gcggggcggg agcaaacaag ttttctggca gatagccttt ccacaggctg tgcattgagt
     2101 tgtccggatc gcacaccgac atcgcgccaa aggtatcgcc atgataacca ttgcggaagg
     2161 tcagaaaacg ctggcgcgct tcgcctttgg cttgccagta ctgcaacgcc attttcatcg
     2221 ccacttccac cgctacggaa ccggagtccg cgagaaaaac gcactccagc ggttgcggcg
     2281 tcattgccac cagtttgcgg catagctcga tggcgggcgc atgggtgata ccgccaaaca
     2341 tcacatgcga catggcatca atttgcgtct tcatcgccgc attaagctgc gggtgattgt
     2401 agccgtggat cgccgcccac caggacgaca taccgtcaac caggcgtctg ccgtcagaca
     2461 aaattagctc gcaaccttcg gcgctcgcca ccggataaac cggcagaggg gaggtcatgg
     2521 atgtgtatgg gtgccagata tggcgttggt caaaggcaag atcgtccgtt gtcataatcg
     2581 acttgtaaac caaattgaaa agatttaggt ttacaagcct acaccgaatt aacaacaaaa
     2641 aacacgtttt ggagaagccc catggctcac cgcccacgct ggacattgtc gcaagtcaca
     2701 gaattatttg aaaaaccgtt gctggatctg ctgtttgaag cgcagcaggt gcatcgccag
     2761 catttcgaac ctcgtcaggt gcaggtcagc acgttgctgt cgattaagac cggagcttgt
     2821 ccggaagatt gcaaatactg cccgcaaagc tcgcgctaca aaaccgggct ggaagccgag
     2881 cggttgatgg aagttgaaca ggtgctggag tcggcgcgca aagcgaaagc ggcaggatcg
     2941 acgcgcttct gtatgggcgc ggcatggaag aatccccacg aacgcgatat gccgtacctg
     3001 gaacaaatgg tgcagggggt aaaagcgatg gggctggagg cgtgtatgac gctgggcacg
     3061 ttgagtgaat ctcaggcgca gcgcctcgcg aacgccgggc tggattacta caaccacaac
     3121 ctggacacct cgccggagtt ttacggcaat atcatcacca cacgcactta tcaggaacgc
     3181 ctcgatacgc tggaaaaagt gcgcgatgcc gggatcaaag tctgctcagg tggcattgtg
     3241 ggcttaggcg aaacggtaaa agatcgcgcc ggattattgc tgcaactggc aaacctgccg
     3301 acgccgccgg aaagcgtacc aatcaacatg ctggtgaagg tgaaaggcac gccgcttgcc
     3361 gataacgatg acgttgatgc ctttgatttt attcgcacca ttgccgtagc gcggatcatg
     3421 atgccgacct cttacgtgcg cctttctgcc ggacgcgagc agatgaacga acagactcag
     3481 gcgatgtgct ttatggcagg cgcaaactcg attttctacg gttgcaaact gctgaccacg
     3541 ccgaatccgg aagaagataa agacctgcaa ctgttccgca aactggggct aaatccgcag
     3601 caaactgccg tgctggcagg ggataacgaa caacagcaac gtcttggaca ggcgctgatg
     3661 accccggaca ccgacgaata ttacaacgcg gcagcattat gagctggcag gagaaaatca
     3721 acgcggcgct cgatgcgcgg cgtgctgccg atgccctgcg tcgccgttat ccggtggcgc
     3781 aaggagccgg acgctggctg gtggcggatg atcgccagta tctgaacttt tccagtaacg
     3841 attatttagg tttaagccat catccgcaaa ttatccgtgc ctggaagcag ggggcggagc
     3901 aatttggcat cggtagcggc ggctccggtc acgtcagcgg ttatagcgtg gtgcatcagg
     3961 cgctggaaga agaactggcc gagtggcttg gctattcgcg ggcgctgctg tttatctctg
     4021 gttttgccgc gaatcaggca gtcattgccg cgatgatggc gaaagaggac cgtattgttg
     4081 ccgaccggct tagccatgcc tcattgctgg aggctgccag tttaagcccg tcgcagcttc
     4141 gccgttttac tcataacgat gtcgctcatc tggcgcgact gcttgcttcc ccctgtccgg
     4201 ggcagcaact ggtggtgaca gaaggcgtgt tcagcatgga cggcgatagc gcgccactgg
     4261 cggaaatcca gcaggtaacg caacagcaca atggctggtt gatggtcgat gatgcccacg
     4321 gcacgggcgt tatcggggag caggggcgcg gcagctgctg gctgcaaaag gtaaaaccag
     4381 aattgctggt agtgactttt ggcaaaggat ttggcgtcag tggggctgcg gtgctttgct
     4441 caagtacggt ggcggattat ctgctgcaat tcgcccgcca ccttatctac agcaccagta
     4501 tgccgcccgc tcaggcgcag gcattacgtg cgtcgctggc ggtcattcgc cgtgatgagg
     4561 gggatgcacg gcgcgaaaaa ctggtgtcac tcattgcgcg ttttcgtgcc ggagtacagg
     4621 atttgccgtt tacgctggct gattcatgca gcgccatcca gccattgatc gtcggtgata
     4681 acagccgtgc gttacaactg gcagaaaaac tgcgccagca aggctgctgg gtcacggcga
     4741 ttcgcccgcc aaccgtaccc gctggtactg cgcgactgcg cttaacgcta accgctgcgc
     4801 atgaaatgca ggatatcgac cgtctgctgg aggtgctgca tggcaacggt taataaacaa
     4861 gccattgcag cggcatttgg tcgggcagcc gcacactatg agcaacatgc agatctacag
     4921 cgccagagtg ctgacgcctt actggcaatg cttccacagc gtaaatacac ccacgtactg
     4981 gacgcgggtt gtggacctgg ctggatgagc cgccactggc gggaacgtca cgcgcaggtg
     5041 acggcgttag atctctcgcc gccaatgctt gcgcaagccc gccataaaga tgccgcagat
     5101 cattatctgg caggagatat cgaatccctg ccgttagcga ctgcgacgtt cgatcttgca
     5161 tggagcaatc tcgcggtgca gtggtgcggt aatttatcca cggcactccg cgagctgtat
     5221 cgggtggtgc gccccggagg cgtggtcgcg tttaccacgc tggtgcaggg atcgttaccc
     5281 gaactgcatc aggcgcggca ggcggtggac gagcgtccgc atgctaatcg ctttttaccg
     5341 ccagatgaaa tcgaagagtc gctgaacggc gtgcgttatc aacatcatat tcagctcatc
     5401 acactatggt ttgatgatgc gctcagtgcc atgcgttcgc tgaaaggcat cggtgctacg
     5461 catcttcatg aagggcgcga cccgcgaata ttaacgcgtt cgcagttgca gcgattgcaa
     5521 ctggcctggc cgcaacaaca ggggcgatat cctctgacgt atcatctttt tttgggagtg
     5581 attgctcgtg agtaaacgtt attttgtcac cggaacggat accgaagtgg ggaaaaccgt
     5641 cgccagttgt gcacttttac aagccgcgaa ggcagtaggt tatcggacgg caggttataa
     5701 accggtcgcc tctggcagcg aaaagacccc ggaaggctta cgtaatagtg acgcgctggc
     5761 gttacagcac aacagcagcc tgcagctgga ttacgcaacg gtaaatcctt acaccttcgc
     5821 agaatccacc tcgccgcaca tcatcagcgc ccaagagggc aggccgatag aatcattggt
     5881 aatgagcgcc ggattacgcg cgcttgaaca acaggctgac tgggtgttag tggaaggtgc
     5941 tggcggctgg tttacgccgc tttctgacac tttcactttt gcagattggg taacacagga
     6001 acaactgccg gtgatactgg tagttggtgt gaaactcggc tgtattaatc acgcgatgtt
     6061 gactgcgcag gcaatacaac acgccggact gactctggcg ggttgggtgg cgaacgatgt
     6121 tacgcctccg ggaaaacgtc acgctgaata tatgaccacg ctcacccgca tgattcccgc
     6181 gccgctgctg ggagagatcc cctggcttgc agaaaatcca gaaaatgcgg caaccggaaa
     6241 gtacataaac cttgccttgt tgtagccatt ctgtatttgg ttaaattgcg agcgagatcg
     6301 cgtcttcgat tgactgcaat ttaaccaatt aaattctaaa ataatcacga aaaaaatttt
     6361 acttccgcct catgcggcga atgtgggaat tgcccaggcg gcgggggata ggggctggag
     6421 acagttatcc actattcctg tggataacca tgtgtattag agttagaaaa cacgaggcaa
     6481 gcgagagaat acgcggcttg cacgcgaatt ggcgttaaag acggctcaaa gaaatatctt
     6541 ttatttttta actggttaga taaatgcaat ggcagtcact gaacaggcat ctcttgccat
     6601 aaaactgtca tcactcatct tgacaaatgt taaaaaagcc gttgctttgg ggataacccg
     6661 gtaaggctgg agttttatct cgccacagag taaattttgc tcatgattga cagcggagtt
     6721 tacgctgtat cagaaatatt atggtgatga actgtttttt tatccagtat aatttgttgg
     6781 cataattaag tacgacgagt aaaattacat acctgcccgc ccaactcctt caggtagcga
     6841 ctcatgagta aaccgttcaa actgaattcc gcttttaaac cttctggcga tcagccagag
     6901 gcgattcgac gtctcgaaga ggggctggaa gatggcctgg cgcaccagac gttacttggc
     6961 gtgactggct ccgggaaaac cttcaccatt gccaatgtca ttgctgacct tcagcgccca
     7021 accatggtac ttgcgcccaa caaaacgctg gcggcccagc tgtatggcga aatgaaagag
     7081 ttcttcccgg aaaacgcggt ggagtatttc gtctcctact acgactacta tcagccggaa
     7141 gcctatgtac cgagttccga caccttcatt gagaaagatg cctcggttaa cgaacatatt
     7201 gagcagatgc gtttgtccgc caccaaagcg atgctggagc ggcgtgatgt gattgtagtg
     7261 gcgtctgttt ccgcgattta tggtctgggc gatcctgatt tatatctcaa gatgatgctc
     7321 catctcacgg tcggtatgat tatcgatcag cgcgcgattc tgcgccgact ggcggagctg
     7381 caatacgctc gtaatgatca agcattccag cgtggtactt tccgcgttcg tggcgaggtg
     7441 atagatatct tcccggcaga atcggatgac attgcacttc gcgtggaact gtttgacgag
     7501 gaagtggaac gattgtcgtt atttgacccg ctgaccgggc agattgtttc cactattcca
     7561 cgttttacca tctacccgaa aacgcactac gtcacaccgc gcgagcgcat cgtacaggcg
     7621 atggaggaga tcaaagaaga actggccgcc agacgcaaag tgctgttgga aaacaacaaa
     7681 ctgctggaag agcagcggct gacccagcgt acccagtttg atctggagat gatggacgag
     7741 ctgggctact gttcggggat tgaaaactac tcgcgcttcc tctccggtcg tggaccgggt
     7801 gagccaccgc cgacgctgtt tgattacctg cctgccgatg ggctgctggt cgtcgatgaa
     7861 tctcacgtca ccattccaca aattggcggc atgtatcgcg gtgaccgggc gcgtaaagag
     7921 acactggtgg agtacggctt ccgcctgcca tcagcgctgg ataaccgtcc gcttaagttt
     7981 gaagagttcg aagcattagc gccgcaaacc atctatgttt cggcgacgcc gggtaattac
     8041 gagctggaaa aatccggcgg cgatgtggtg gatcaggtgg tgcgtccaac cggattgctt
     8101 gacccgatta tcgaagtgcg gccggtggcg acacaggttg atgatcttct ttcggagatt
     8161 cgtcagcgag cggcaattaa cgaacgcgtg ctggtcacca cactgaccaa gcgaatggcg
     8221 gaagatctta ccgaatatct cgaagaacac ggtgagcgcg tgcgttatct tcactcagat
     8281 atcgacaccg tcgagcgtat ggagattatc cgcgacttgc gtctgggtga gttcgacgta
     8341 ttggtaggga tcaacttact gcgtgaaggt ctggatatgc cggaagtgtc gctggtggcg
     8401 atcctcgacg ctgacaaaga aggcttcctg cgttccgaac gttcgttgat ccagaccatt
     8461 ggtcgtgcgg cacgtaacgt taacggtaaa gcgattctct acggcgatag gagcactcca
     8521 tcaatggcga aagcgattgg cgaaaccgaa cgtcgccgtg agaaacagca gaagtacaac
     8581 gaggaacacg gcattacgcc gcaaggcttg aacaagaaag tggtcgatat cctggcgctg
     8641 gggcagaaca ttgccaaaac caaagcgaag ggcagaggaa aatcgcgccc gattgttgag
     8701 ccggataatg tgccgatgga tatgttgccg aaagcgttgc agcagaaaat ccatgaactg
     8761 gaagggctga tgatgcaaca cgcgcagaat ctggagttcg aagaagcggc acaaattcgt
     8821 gaccagttgc atcagttgcg tgagttgttt attgccgcat cgtaacagga tagcgaagaa
     8881 gactgatgac aaacggaaaa cagtctgatg cgctacgctt atcaggccta cattttctcc
     8941 gcaatatatt gaatttgcgc ggtttgtagg ccggataagg cgttcacgcc gcatccggca
     9001 taaacaacgc gcactttgtc ttcaatagga agccggaatt ttccttcgga tttccgttaa
     9061 cctaaagcct gtaacgcctt ttccagcgcg ttatgtaaca actggcggtc atgacgatac
     9121 ggaatatcgc tggcctccag tacctcctgg atcacaatcc gctctttcac cgccgagaca
     9181 tccacttttg gcccgacgat gaccgcatca atgacctttt taccaacata ctgctccata
     9241 attgccagct tgctttccag cttcaaatta gccgcaggta aactcaactc acgccccaga
     9301 ttgccgatat aaaccatcgg cgctggcgtg cggcgtaatg cctgggcgat ttccttcagc
     9361 agcagaattg gcatcaggct ggtataaaaa ctgccaggcc caataatgat gagatccgct
     9421 tcattgatag cgtgaaccgc ctcacgcgtt gcgggtacat taggcgttaa caataactct
     9481 tgaatcggcg tagttaactg gtcgatattg acctcgccgt aaacttcatg cccctgatcg
     9541 tcaatcgcca tcagatcaac aggatgctct gacattggaa tcaaatgcgt atccactttc
     9601 agcagattac gaattaaatt gatggcttcc agaggccgca cgctaaggtg atccagcgcc
     9661 tttaacatca agtttccgag attatgaccg gaaagttcgc cattgccgcc aaaacggtat
     9721 tcaaacatcg cggaggcgac gttcggttcc gttatcagct ggttgaggca gttgcgcata
     9781 tcgccccaag caatgccgcc ttctgaacgg cgaatacgcc ccgtcgagcc accattatcc
     9841 gtggtggtga cgatacccgt taaacgagaa cccaaagacg aaagtgatga gagaacgcgt
     9901 cccagtccat gccctccgcc gagagcaacg acacgatcaa gatcagccag cgtacgattg
     9961 cgcatatagt ttcctggagt cagattatcc gcgctacagt agcgcaaatt gcggggaaac
    10021 agcaattaac ctgccaatat tgaggataca aactattttc ttctatctct aaaggacgat
    10081 gcacgctatg cctccctgat gatgtatatc aaagtaaaac cgccattttc ccttattctg
    10141 tagcgaaata gcacgatcat gacgctatat acatgattac atagcgaaag tgtggatggt
    10201 aaaaatctca tttacacgct agtatcggct taaccactaa acactctagc ctctgcacct
    10261 gggtcaactg atacggtgct ttggccgtga caatgctcgt aaagattgcc accagggcga
    10321 aggaagaaat gacttcgcct cccgtatctg gaaaggtgta catggcttca caactgactg
    10381 atgtatttgc gcgtaagttt tactacttgc gcctgtcgat taccgatgtg tgtaactttc
    10441 gttgcaccta ctgcctgccg gatggctaca aaccgagcgg cgtcaccaat aaaggctttc
    10501 ttaccgtcga tgaaattcgc cgggttacgc gcgccttcgc cagtctgggc accgaaaaag
    10561 tgcgcctgac aggaggagag ccgtctttac gccgcgactt taccgatatc atcgccgctg
    10621 tgcgggaaaa cgacgctatc cgccagattg cggtcaccac caatggttac cgtctggaac
    10681 gcgatgtggc gaactggcgc gatgcgggac ttactggcat taacgtcagt gtcgacagtt
    10741 tggacgcccg ccagtttcac gctattaccg ggcaggataa attcaaccag gtcatggcag
    10801 ggattgatgc tgcatttgag gccggttttg agaaggtcaa agtcaatacc gtgctgatgc
    10861 gtgatgttaa tcatcaccag ctcgacacct ttctgaactg gatccagcat cgccctatcc
    10921 agctgcgttt catcgaactg atggaaacgg gcgagggcag cgagctcttc cgtaagcatc
    10981 acatctctgg tcaggttctg cgtgacgagc tactgcgtcg cggctggatc caccaattac
    11041 gtcaacgcag cgacggtccc gcgcaagtct tttgccatcc agattacgcc ggagagattg
    11101 gccttatcat gccgtatgaa aaagacttct gcgccacttg caaccgcctg cgcgtttcct
    11161 ccattggtaa actccatctc tgcctgtttg gtgaaggcgg cgttaacctg cgcgatctgc
    11221 tggaaggcga tacccagcaa caggcgctgg aagcgcgtat ttcagcggcg ctgcgggaga
    11281 agaaacagac ccatttcctg catcaaaaca acaccggtat tacgcaaaac ttatcgtaca
    11341 ttggcggcta aaacgtcaaa aggagagatc agatgagtca ggtaagcact gaatttatcc
    11401 cgacccgtat tgctattctt acggtttcta atcgtcgcgg tgaagaagac gatacctccg
    11461 gtcactatct gcgcgattcg gcgcaagaag cgggccatca cgttgtcgat gaagccattg
    11521 tgaaagaaaa ccgctacgct attcgcgctc aggtatctgc gtggatcgcc agcgacgatg
    11581 tacaagtggt attgattacg ggtggtactg gcctgacgga aggtgatcag gctcccgaag
    11641 cgctgctgcc gttgttcgac cgtgaggttg aaggttttgg tgaagtgttc cgtatgttgt
    11701 cgtttgaaga gattggcact tccacgttgc aatctcgtgc ggtagcgggc gtcgctaaca
    11761 aaacgctgat tttcgccatg ccgggttcga ccaaagcgtg ccgtaccgca tgggaaaata
    11821 tcatcgcgcc gcagctggat gcccgtacgc gtccgtgtaa tttccatcca catttgaaga
    11881 aataagtatg tcgcaactga cccatatcaa cgccgctggc gaagcgcaca tggtggatgt
    11941 ctccgccaaa gcggaaaccg tgcgtgaagc gcgggcggaa gcctttgtca ccatgcgcag
    12001 cgagacgctg gcgatgatta ttgatggtcg ccaccacaaa ggcgacgtat ttgccactgc
    12061 gcgtattgcc ggtattcagg cggcaaaacg cacttgggat ctgatcccgc tgtgtcaccc
    12121 gctgatgctc agcaaagttg aagtcaattt acaggccgag ccggagcaca atcgggtgcg
    12181 tatagaaacc ttatgccgcc tgaccgggaa aaccggtgtc gaaatggaag cgttaaccgc
    12241 ggcctccgtg gcggcgctga ccatttatga catgtgcaaa gcggtgcaaa aagatatggt
    12301 gattggtccg gtacgtttgc tggcgaagag cggcggcaag tcgggtgact ttaaggtgga
    12361 agcggatgat taaagttctt tttttcgccc aggtgcgcga gttggtggga acagatgcaa
    12421 ccgaagtggc tgcggattac ccaaccgttg aagcgttacg ccagcacctg gctgcgcaga
    12481 gcgatcgctg ggcgctggcg ctggaagatg gcaaattgct ggctgccgtc aaccagacgc
    12541 tggtgagttt tgaccatccg ctgactgacg gcgacgaagt agctttcttc ccgccggtaa
    12601 ccggaggtta agatggcaga aaccaaaatt gttgtcggcc cgcagccgtt cagcgtagga
    12661 gaagagtacc cgtggctggc ggagcgtgac gaagacggtg cggtagtcac cttcaccgga
    12721 aaggtgcgca accataacct gggcgacagc gtcaaagcat taaccctcga acactatccg
    12781 gggatgactg aaaaagcact ggcagaaatt gttgatgaag cgcgtaaccg ctggccgctg
    12841 gggcgcgtca cggtgattca ccgcatcggg gaattatggc cgggcgatga aatcgttttt
    12901 gtcggtgtca ccagtgcgca tcgcagcagt gcgtttgaag ccgggcagtt tattatggat
    12961 tatctcaaaa cccgcgcacc gttctggaag cgcgaagcca cgccggaagg cgaccgctgg
    13021 gttgaagccc gggagagcga tctgcaggcg gcaaaacgct ggtagttttt tgttagccgg
    13081 ataaggcacc gctgcgtccg gcaatttcac cgctcagtac atgcgtttac gctgcgtcga
    13141 tgcacagcct catcattttg cagtatcctt aagatattcc ttatatcttc aggagatcgt
    13201 catggacaga ttcccacgtt ctgattcaat cgtacaaccc tgggctggct tgcaaactta
    13261 tatggcgcaa gtctatggct ggatgaccgt tggcttgttg ctgaccgcat ttgttgcctg
    13321 gtatgcggct aattccgcgg ccgtgatgga gctgttgttc actaaccgtg tctttttaat
    13381 cggtctgatc atcgcgcaat tagcattggt tattgtgtta tcagcgatga ttcaaaagct
    13441 gagcgcaggt gtaacgacga tgctctttat gctttattcg gcgctgacgg gtcttacgct
    13501 ttccagtata ttcattgtct ataccgctgc ttctatcgcc agtactttcg tcgttactgc
    13561 cgggatgttc ggcgcaatga gcctgtacgg ttacaccacg aagcgcgatt taagtggctt
    13621 tggcaatatg ctgtttatgg cgttaatcgg cattgtgctg gcatcgctgg tcaacttctg
    13681 gctgaaaagc gaagcattga tgtgggcagt tacctacatc ggcgtgattg tctttgtcgg
    13741 attgacggcg tatgacactc agaaattgaa aaatatgggt gagcagattg atacccgcga
    13801 cacgtcgaac ctgcgcaaat attccattct tggtgcgtta accttgtatc tggacttcat
    13861 caacctgttc ctgatgttgt tacggatctt cggcaaccgc cgttaattct ttttcaccat
    13921 cactatcccg gccatgtgtc gggatttttt tcttactaaa aacgacactt aacaagtacc
    13981 aggtataaaa tatgccttat gagcgagcgg aatcgttttc cgtatccata aatacaaaag
    14041 tattttgtat gcgtggtttt aaatttattg atggtgaata ttaatgtttt ttctaaggac
    14101 aactaaaaga atggaatcat actcgcaaaa cagcaataaa ttagattttc agcacgaggc
    14161 caggatatta aacggtatat ggctcattac cgctttaggt ttggtggcaa ccgcaggact
    14221 agcctgggga gctaagtatc tcgaaattac ggcaaccaaa tatgattcac caccaatgta
    14281 tgtcgccata gggttattat tactttgtat gtatggctta agtaaggata tcaacaagat
    14341 aaatgccgcc atcgcgggcg taatatatct gtttttactc tctttggtgg cgattgtcgt
    14401 tgcaagttta gttcctgtat atgccattat catcgtgttc agcactgcgg gcgcgatgtt
    14461 tttaatcagt atgctggccg gtttattatt taatgttgat cctggttctc accgttttat
    14521 cattatgatg acgttgacag ggttggccct ggtaatcatc gtgaatgcgg cattaatgag
    14581 tgaacggccc atttgggtaa taagttgctt aatgattgtg ttatggccag gcattatctc
    14641 gcatggacga aataagctcc ttgaactggc aggaaaatgc catagtgaag agttgtggag
    14701 tccggttcgt tgcgctttta caggtgcatt aacactctat tactatttta tcggcttctt
    14761 tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc gtttttttca
    14821 ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg cttcattttt
    14881 cgcccgtagc ttcttcgctt ggctttccag tagcaggtaa cagatcagcg ccagcagcag
    14941 cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc tccctttcga
    15001 ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga tccccgccgg
    15061 aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta caaagaaata
    15121 gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc agttaacgct
    15181 ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct tttgcccttt
    15241 gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat
    15301 cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt aatccggcaa
    15361 ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca gccagttggt
    15421 ggtaatactg agcgaggaaa tccgcgtaat agtgctgccc ggtaacccta gtcgggagta
    15481 caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt tgaaggcgta
    15541 gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc agtaaaagcg
    15601 ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc cgaccgcact
    15661 aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc agtccacttt
    15721 tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg tgaggatctt
    15781 ctttgctaag cgccagcgcg ggtgtgattt actcatcagg ggtttacccc cgtgttttca
    15841 gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg ggggatcaac ctgcgtcaga
    15901 cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa aaagtggaac
    15961 gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt ttcatccacc
    16021 tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg gttaaaatga
    16081 cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg atcgagatta
    16141 ctggacccta ctgtcgccca gtgatcgtcc atcaatgcca ctttgccgtg gagcgggcgg
    16201 cggcggtact caaaaacctg aacgccgcct ttaaccagat agttatacag caagcgcgca
    16261 ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat ccgcaccccg
    16321 cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg
    16381 gcgataatca cttcccgctg cgcctgagtg agcattttca gataatggcg ttcaatatca
    16441 tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc cggctggcgg
    16501 ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg
    16561 ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa
    16621 tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt cagcccgcca
    16681 ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg aaacacattg
    16741 gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac gccagctgcc
    16801 gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc cagcaagact
    16861 tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg tttgccgacg
    16921 ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc
    16981 ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt attgccttcg
    17041 cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc
    17101 gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg acgtagatcc
    17161 tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc
    17221 gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct ttttgccgcc
    17281 agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca ttcacccatt
    17341 cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga tgtacgcaca
    17401 tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct
    17461 tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga taacgcgaca
    17521 gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc
    17581 tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt tccacatgca
    17641 gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg gcgctgacgg
    17701 tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt
    17761 tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg ggcataattt
    17821 tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt
    17881 cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat tcttcaggag
    17941 agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt gctggcagat
    18001 tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta agactctggt
    18061 tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt
    18121 tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag agctgaccac
    18181 gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa
    18241 caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg tagtgtcttt
    18301 gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac gccgctgacc
    18361 ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata tccagactcg
    18421 catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg
    18481 tcagattttg cagccacact ggcatgtttt ccaccggcga aacgtaaccg gaaaggagaa
    18541 tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc gagcagagtg
    18601 atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc gtaaagtaga
    18661 acagcgctag cgatccggcg aaggggattt gatacgccca gataccaatc gccagcacaa
    18721 tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg aagatctgcc
    18781 aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga
    18841 cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc agcgacggca
    18901 ccacaaacca tttgtagtcg agattcgggt tataccagtt gcgtaccacc agctcgctgt
    18961 tgttgggttt cggttttcct tccagcagct cctgctgata atttttgacg atctgttgca
    19021 ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc aactgcaacg
    19081 gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc accagtagta
    19141 acgccttttg tgtgtcgatg gtcgggcgga tctcctgtgg gcttttcagc agcagcacat
    19201 gtgtaaaggc gctggcgcgg gcaaaacgtt gagtaagttc caccgaatgc tcgccgttat
    19261 cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg aacgggaaca
    19321 ggatcacctg aattagcacg ggtaaaatca gaatcgcgcg ggtttgtggt tcgcgcagca
    19381 acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc ttctccctaa
    19441 tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc cgaagcgatc
    19501 aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa caggctttgc
    19561 agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat caccgcgggc
    19621 atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcagaaa agcggcgttc
    19681 agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag cagccccatt
    19741 cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag cgaaccgcga
    19801 tacggcacgc cgagaataaa cactgacacc agcatacaca gcaacatcgc cagcatcccg
    19861 agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat ctccgtagag
    19921 agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc
    19981 gcgccgatga ccgtcatgat aatggtcacc gcacccggga taataaagtg ctggcttatc
    20041 gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa agtctgcccg
    20101 ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata cccctgcaca
    20161 aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg
    20221 gcgcgctcca tctgttcggc gaaatccacg ggaataacca ccagaccgcg aattttcccc
    20281 gactgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc
    20341 gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc cagcaaaatc
    20401 ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa cagcagtagc
    20461 agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg ccgcgtctct
    20521 ttgacgcaca gcgcccgcac gcgatgccag gaaaggatcg ggttactcat tgctatgctc
    20581 cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat cgggttgctc
    20641 atcgttagct gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta atttcccacg
    20701 gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg tggtgaccat
    20761 caccgtgacg cctttctcta ccatgctgtt gatgtgcagc caaaattcac ggcgggtgag
    20821 ggggtcaacg ccggaagtcg gttcgtcgag aaagagaata tccggttcat gcatcagcga
    20881 acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg cgtgtgaggc
    20941 aatacttttc aggccaaatg cctcgctcat acgggagatt ttttcgttct gcgcccgacc
    21001 gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca ggttgccgta
    21061 gagcgaaaat ttttgcgcca tatagccgag atgctggcgc gctttaccgg aactctcttt
    21121 cagatccatc cccagcacca gcgcctggcc ggaagtcggc accagcaaac cgcacatcat
    21181 cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa tctccccacg
    21241 tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct tggtcagttc
    21301 tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgcaata ttgcacccag
    21361 cggcgattct gaggttccgg caccgcccag caaatcaata aacgcatctt caaaacgcgg
    21421 cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat cgtctggtgt
    21481 ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccat cgctgacctg
    21541 cggcagtttc aaggcgcgtt gcaacagttt gcggttgccc tcgtgtggac tggtcatcag
    21601 aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tctccctgat acagcaactc
    21661 gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat acgaggtact
    21721 ccagaggatt aacatccctt cgcccgccag ctcatgcacc atctgccaca gttcgcgccg
    21781 tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg gttcgcccac
    21841 cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc ccgccaggcg
    21901 tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc
    21961 ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca ccgtgagatc
    22021 ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt
    22081 tttgatcgga tcaaagccaa tcaccgtggc actgccgctg tcgggtttca gtaatcccgc
    22141 caacatccgc atcagtgtgg ttttacctgc accgtccggc cccaccaacc ccgtcacata
    22201 accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc ccggaaagcg
    22261 tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg tcaccgaatt
    22321 gtaccgtcac tggcattccc tggcgtaacg catcatcggc gtcggtcacc acaatacgca
    22381 ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg gtaaattcag
    22441 cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga ccatcggtat
    22501 aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca acataagcgc
    22561 gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca ttgagaaccg
    22621 tgcctggctc taccgcgcgc gttaacagcg tgccatcaga cggtgctatc aacgttgagt
    22681 cctgtaaatt cagctccgcc tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg
    22741 cctgagcgat gtcctgttca cggttaccgg aacggtactg acgcaattta tcctgtgctg
    22801 atttcagcgt tgcctgtgcc tggtcgcgcg aggagcgggc attttccagg tcatttgccg
    22861 aaatggtgcg gcttttccac agtccttgct ggcggttata gaagttctgc gcatagtcat
    22921 aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca tcgcgatacc
    22981 cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc gcctgcatca
    23041 gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct ttgatagcat
    23101 caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta agattcaccg
    23161 tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt tgataccacc
    23221 agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc acgacaggtt
    23281 ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga tatgacaggt
    23341 caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa
    23401 cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg catgggtatg
    23461 aaggatcatg cgggtgtcat tagcgtcgca gccggtccag gcggcaatca ggcgtgtcag
    23521 gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga
    23581 gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg tgagcagctt
    23641 aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gcagcccgat caggctgtgg
    23701 ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc caataaaatc
    23761 ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt agtaggtgat
    23821 ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt tcattccata
    23881 ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt cacccttgat
    23941 tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg attaagatta
    24001 tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca gtcacaggga
    24061 taatttatgc gctgcgtcac aaaaactgct acactccgct ccctcatgac attgtggttt
    24121 ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc ggggcggttc
    24181 ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg cgccgttgcc
    24241 gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc tgtgctggaa
    24301 ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg ctttacgctg
    24361 ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg tccggtacgt
    24421 gcgctcattc ttaccccgac ccgtgaactg gcggcacaga ttggcgaaaa cgtccgtgat
    24481 tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gcggtgtcag cattaaccca
    24541 caaatgatga agttgcgcgg cggcgttgat gtgctggtgg caacgccggg acgtttgctg
    24601 gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa
    24661 gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg
    24721 ccagcgaaac gccagaacct gctgttctct gcgaccttct ctgacgatat taaagccctg
    24781 gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct
    24841 gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga attgctgtcg
    24901 cacatgattg gtaaagggaa ctggcagcag gtgctggtgt ttacccgtac caaacacggc
    24961 gctaaccatc tggctgagca gctcaataaa gatggcatcc gtagtgtggc gatccacggg
    25021 aataaatcgc aaggtgcgcg tacccgtgcg ctggctgatt ttaaatcggg tgacattcgc
    25081 gtactggtgg cgaccgatat cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc
    25141 gtcaactatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg gcgtaccggt
    25201 cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg
    25261 cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc gggctatgag
    25321 ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg tggcggcatc
    25381 aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacgtgaact
    25441 ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac gccgcatccg
    25501 gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt
    25561 accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg caactccatt
    25621 aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatcg
    25681 ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac acgcagttct
    25741 gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt aaccgactcc
    25801 cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac catgggggaa
    25861 gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctgaaaatag
    25921 tgttcggagg tgggccaggt attcccgtca accttgatgg gcctggcggc aaagttagaa
    25981 aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg catgacgtgt
    26041 tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagccg
    26101 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
    26161 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
    26221 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
    26281 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
    26341 tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc
    26401 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
    26461 ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc
    26521 tttcgggcgt gggcgctacg tttgtccacg taatctgacg gcgctcgcca gcactgaacc
    26581 cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga
    26641 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
    26701 cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta ccgacaaagc
    26761 cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg ttactcgtcg
    26821 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
    26881 aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg aaggtcatca
    26941 cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg tgccctggta
    27001 tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
    27061 gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
    27121 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
    27181 agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct gccagcggct
    27241 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
    27301 gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga tgaaccgcgc
    27361 gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc
    27421 cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg
    27481 gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
    27541 tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca
    27601 ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
    27661 taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc cttccatcga
    27721 caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg tggagagcaa
    27781 aaaatatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc gctttctcga
    27841 ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc gttatcgctt
    27901 agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg tgggcttaca
    27961 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa
    28021 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
    28081 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
    28141 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta tctacgacaa
    28201 acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
    28261 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
    28321 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
    28381 cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
    28441 cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
    28501 gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
    28561 gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc
    28621 cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga ttctgctgca
    28681 taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc gcgtgctgac
    28741 agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc aggcgcaggc
    28801 gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga
    28861 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
    28921 actggcgacg ccatttgccg aaggtgaggc actgtgtctt tccagcgttt cgcatccgga
    28981 atacattgga cgcgtcgcga agttctttag cgatatcggc gggtgggcgt tactaatgca
    29041 tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga
    29101 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc
    29161 acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcctgg ctggcagcga
    29221 agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
    29281 ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc
    29341 ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
    29401 gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
    29461 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt tattcaggct
    29521 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc
    29581 gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag ctatgtgcgt
    29641 tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
    29701 gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg
    29761 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
    29821 catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg gtttgtctct
    29881 atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc
    29941 cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt cccgctgttg
    30001 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
    30061 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
    30121 gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa aggctacgcc
    30181 cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag
    30241 gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat catcatcaac
    30301 ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc cgagtgggtg
    30361 aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagtgaac
    30421 acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt
    30481 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
    30541 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
    30601 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
    30661 cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
    30721 tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc gtcctacgaa
    30781 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
    30841 gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag
    30901 gggggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
    30961 cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca tagcagcaac
    31021 aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt
    31081 gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga
    31141 ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt tatttgttgt
    31201 acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc acggaatgcc
    31261 acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac gtaactgtct
    31321 gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat ctttctcctg
    31381 ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt gcgggatggg
    31441 ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac cccgagcgcg
    31501 ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca
    31561 agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt agctaatata
    31621 gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat
    31681 aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
    31741 tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc atcgactgga
    31801 tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc
    31861 tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt ccaaaggtgt
    31921 cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag
    31981 aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca tccacatgga
    32041 aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtacgcggca
    32101 aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca ttttgcagtg
    32161 cggcgtataa cgcgctgcgg gtgtctactt gctgattgtt cttaacttgt gcaccttgtg
    32221 ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaatggg
    32281 cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt ctccaaaaag
    32341 tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca agaaggttct
    32401 tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc
    32461 aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat accccagttt
    32521 ggcatcggcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc cgtctgaacc
    32581 tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat cggttgcctg
    32641 atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg aggaaccatc
    32701 ctgggcaaca tctttgccgt ttttgatggt tgctttttgc tgggtatagc cgccaatcac
    32761 ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga cgcgtttctt
    32821 accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga tatcagtacg
    32881 gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc
    32941 gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac cactgccaga
    33001 ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata
    33061 gacattgccg ttttccgtca ggtggtacag cgccccggct ttccagttca ccagattgcc
    33121 cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag ctgggcaggt
    33181 gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt
    33241 atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc
    33301 gtaaattgcg aaggtatccg tctgaccatt ggtgtttgcg ccgttgcgcg tcaggccgcc
    33361 gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca ccggattaac
    33421 gccatagtta gtctgcgttt cacgggtaaa ttccacgccg gtactgacat catgaccgat
    33481 agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac
    33541 atctttggta ttcgccgtgc gtgactaggt ccagctattc acatcgctgg tgggctgagt
    33601 aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta cgcgcgacca
    33661 acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc
    33721 ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac
    33781 ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc ccggcaaacc
    33841 gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat aattaagata
    33901 caaacgattt gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt aacgctcatt
    33961 tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca ggcgcaccgc
    34021 ggtagtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc
    34081 aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgcttgctga tcatattgat
    34141 cgagcctgtc ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa tcacttcgac
    34201 ctgctcggta ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatac catcaatata
    34261 aatactgtta gaggtatcgg caccacgcat ataaatggcg tcgccagtgg tggagttacc
    34321 gttctcaccc gcaaaaaacg cacccacgcc gg
//
LOCUS       DAJTRW010000038        33890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRW010000038 DAJTRW010000000
VERSION     DAJTRW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..707
                     /locus_tag="OO326_001951"
     CDS             <1..707
                     /locus_tag="OO326_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5966346.1"
                     /translation="DDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDS
                     SQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVP
                     YLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRN
                     GKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTP
                     DSEPTTSVLQGTAQCH"
     gene            718..1809
                     /locus_tag="OO326_001952"
     CDS             718..1809
                     /locus_tag="OO326_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5966347.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1852..2712)
                     /gene="rsmI"
                     /locus_tag="OO326_001953"
     CDS             complement(1852..2712)
                     /gene="rsmI"
                     /locus_tag="OO326_001953"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5966348.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2777..4813
                     /gene="lpoA"
                     /locus_tag="OO326_001954"
     CDS             2777..4813
                     /gene="lpoA"
                     /locus_tag="OO326_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5966349.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4771..5166
                     /locus_tag="OO326_001955"
     CDS             4771..5166
                     /locus_tag="OO326_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5966350.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5186..5776
                     /gene="diaA"
                     /locus_tag="OO326_001956"
     CDS             5186..5776
                     /gene="diaA"
                     /locus_tag="OO326_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5966351.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5786..6361
                     /gene="yraP"
                     /locus_tag="OO326_001957"
     CDS             5786..6361
                     /gene="yraP"
                     /locus_tag="OO326_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5966352.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6475..7515)
                     /locus_tag="OO326_001958"
     CDS             complement(6475..7515)
                     /locus_tag="OO326_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5966353.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7588..8223)
                     /locus_tag="OO326_001959"
     CDS             complement(7588..8223)
                     /locus_tag="OO326_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5966354.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8351..8869
                     /gene="yhbO"
                     /locus_tag="OO326_001960"
     CDS             8351..8869
                     /gene="yhbO"
                     /locus_tag="OO326_001960"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5966355.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8849..9292)
                     /locus_tag="OO326_001961"
     CDS             complement(8849..9292)
                     /locus_tag="OO326_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5966356.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9343..9645
                     /gene="yhbQ"
                     /locus_tag="OO326_001962"
     CDS             9343..9645
                     /gene="yhbQ"
                     /locus_tag="OO326_001962"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5966357.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9632..10135)
                     /locus_tag="OO326_001963"
     CDS             complement(9632..10135)
                     /locus_tag="OO326_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5966358.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10129..10653)
                     /locus_tag="OO326_001964"
     CDS             complement(10129..10653)
                     /locus_tag="OO326_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5966359.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10862..11857
                     /locus_tag="OO326_001965"
     CDS             10862..11857
                     /locus_tag="OO326_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5966360.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11866..12744
                     /locus_tag="OO326_001966"
     CDS             11866..12744
                     /locus_tag="OO326_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5966361.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGTVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12825..13832
                     /locus_tag="OO326_001967"
     CDS             12825..13832
                     /locus_tag="OO326_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5966362.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13950..15194)
                     /gene="mtr"
                     /locus_tag="OO326_001968"
     CDS             complement(13950..15194)
                     /gene="mtr"
                     /locus_tag="OO326_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5966363.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15348..17237)
                     /gene="deaD"
                     /locus_tag="OO326_001969"
     CDS             complement(15348..17237)
                     /gene="deaD"
                     /locus_tag="OO326_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5966364.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17230..17310)
                     /gene="yrbN"
                     /locus_tag="OO326_001970"
     CDS             complement(17230..17310)
                     /gene="yrbN"
                     /locus_tag="OO326_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5966365.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17417..18301)
                     /gene="nlpI"
                     /locus_tag="OO326_001971"
     CDS             complement(17417..18301)
                     /gene="nlpI"
                     /locus_tag="OO326_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5966366.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18410..20545)
                     /gene="pnp"
                     /locus_tag="OO326_001972"
     CDS             complement(18410..20545)
                     /gene="pnp"
                     /locus_tag="OO326_001972"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5966367.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20792..21061)
                     /gene="rpsO"
                     /locus_tag="OO326_001973"
     CDS             complement(20792..21061)
                     /gene="rpsO"
                     /locus_tag="OO326_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5966368.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21210..22154)
                     /gene="truB"
                     /locus_tag="OO326_001974"
     CDS             complement(21210..22154)
                     /gene="truB"
                     /locus_tag="OO326_001974"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5966369.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22154..22555)
                     /gene="rbfA"
                     /locus_tag="OO326_001975"
     CDS             complement(22154..22555)
                     /gene="rbfA"
                     /locus_tag="OO326_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5966370.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22719..25367)
                     /gene="infB"
                     /locus_tag="OO326_001976"
     CDS             complement(22719..25367)
                     /gene="infB"
                     /locus_tag="OO326_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5966371.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25392..26879)
                     /gene="nusA"
                     /locus_tag="OO326_001977"
     CDS             complement(25392..26879)
                     /gene="nusA"
                     /locus_tag="OO326_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5966372.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26907..27359)
                     /gene="rimP"
                     /locus_tag="OO326_001978"
     CDS             complement(26907..27359)
                     /gene="rimP"
                     /locus_tag="OO326_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5966373.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27566..27642)
                     /locus_tag="OO326_001979"
     tRNA            complement(27566..27642)
                     /locus_tag="OO326_001979"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27606..27608),aa:Met,seq:cat)
     gene            27990..29333
                     /gene="argG"
                     /locus_tag="OO326_001980"
     CDS             27990..29333
                     /gene="argG"
                     /locus_tag="OO326_001980"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5966374.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29341..30948)
                     /locus_tag="OO326_001981"
                     /pseudo
     CDS             complement(29341..30948)
                     /locus_tag="OO326_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31426..31512)
                     /locus_tag="OO326_001982"
     tRNA            complement(31426..31512)
                     /locus_tag="OO326_001982"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31476..31478),aa:Leu,seq:gag)
     gene            complement(31527..31859)
                     /gene="secG"
                     /locus_tag="OO326_001983"
     CDS             complement(31527..31859)
                     /gene="secG"
                     /locus_tag="OO326_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5966375.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32087..33424)
                     /gene="glmM"
                     /locus_tag="OO326_001984"
     CDS             complement(32087..33424)
                     /gene="glmM"
                     /locus_tag="OO326_001984"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5966376.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33417..>33890)
                     /gene="folP"
                     /locus_tag="OO326_001985"
     CDS             complement(33417..>33890)
                     /gene="folP"
                     /locus_tag="OO326_001985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5966377.1"
                     /translation="LEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAEVNRYFIEQI
                     ARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGMSRKSMIGQL
                     LNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAKENKRYE"
BASE COUNT         8272 a         9216 c         8377 g         8025 t
ORIGIN      
        1 tggatgatgg tcgcctgagc tatagcctgg aacaaagtct ggatgacgat aacaaccata
       61 acagtagcct gaacgccagt taccgttcac cttatggaac cttcagtgcc ggatacagtt
      121 acggtaatga cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc
      181 atggcgtgac gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaacgggg
      241 cttctggcgt gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag
      301 tggttcctta tctcacgact tatcaggaaa accgtctctc ggtagatact acgcagctgc
      361 ccgataacgt cgatcttgaa caaacaacac agtttgtggt gcccaacaga ggtgcaatgg
      421 tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca
      481 acggtaaacc gttgcccttt ggcgctcttg ccagcaacga tgatacgggg caacaaagta
      541 tcgtcgatga gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga
      601 ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt
      661 cagaaccaac aacctctgta ttacaaggca cagcgcagtg ccattaagga taaaaaaatg
      721 aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc
      781 tcaggagggt gtggagccga cagcactagc ggtgcgacaa attacagcag tgtggttgat
      841 gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc tgcaacgcta
      901 agtagcacta actggcaata cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc
      961 tatatggtca gccccgtact taccaccact ggacatcaga caggatatta caaactcaat
     1021 gacagcctgg atattaaaac cacattacag gcaaacgaca ttccaggact cacaaccgac
     1081 caggttgtct ctgttaacac ccgattcaca cagataaaaa gcagcaccgt atattctgct
     1141 gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt
     1201 gcaaatacca cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg
     1261 aaaacggata ttgccgtcat taaaggcgcg tgggtcgatg gaatgggaag cccgtctaca
     1321 ggtgacttcc atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg
     1381 tgcaaaatta atcagggcga tgttattaaa gttaattttg gattcatcaa tggtcagaag
     1441 tttaccaccc gcaatgccat gccagacggt tttactccag tagactttga tatcacttat
     1501 gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact
     1561 ggggtagtag accagtacaa cctggtcgcc aggcgaagaa gttcagacaa tgtgcccgat
     1621 gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt
     1681 atccttcccg ttgatccttc cgggcatggc accgtcaata tgcgcgcctg gccagttaat
     1741 ctggtcggtg gtgagctgga aacaggaaaa tttcagggca cagccaccat taccgtcatg
     1801 gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg cttcggcact gttacccctg
     1861 ctgctccagc gcatacttat acagcgcatt tttcttcacg ccatgaattt ctgcggccag
     1921 cgccgccgct tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc
     1981 ggcgggtaag tcttcttcct gtgctttatg accttcgaca atcagcacca tttcgccttt
     2041 gcgacggttt tcatcttcct ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat
     2101 ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac
     2161 cgcaacgata tcttccaggc tatctaacag acggtgggta gattcataaa aaatcagcgt
     2221 gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa
     2281 aaagccttcg taacagaaac ggtcagatgg taaacccgcg gcgcttaacg cagtgatagc
     2341 agcacacggt ccgggtagcg gcaccacacg aatccccgct tcacggcagg tacgcaccag
     2401 atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttttg
     2461 cccctcttgc agctttgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag
     2521 cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc
     2581 ctcggcggca atcagatcaa cggcctgtaa tacctctaac gtacgctggg tgatatccgc
     2641 cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg
     2701 gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa
     2761 tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct
     2821 gcctgttgtt ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc
     2881 cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttatcttc agcagatgca
     2941 gcaaagctct gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa
     3001 agaaggtaaa accgggcagg cggttgagtt gtttaaccaa ctaccgcaag aactgaacga
     3061 ttctcagcgt cgcgagaaaa cactgctggc ggcagagatt aaactggcgc agaaagattt
     3121 tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca
     3181 agcgcgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact
     3241 gcgcgcgtta attgctcagg aaccgctgct tggcgcgaaa gaaaaacagc agaatattga
     3301 tgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc tggtgatcaa
     3361 cgccgacgaa aatattctgc aaggctggct ggatctgcag cgcgtctggt ttgataaccg
     3421 taacgatccc gacatgatga aagccgggat cgccgactgg cagaaacgtt atccgaacaa
     3481 tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta aaccagcctc
     3541 gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac
     3601 tattcagcaa ggctttgaag ccgcgaaaaa tatcggcact cagccagtgg cggctcaggt
     3661 agctgccgca cctgccgcag acgtagcaga acaacctcag ccgcaaactg cggatagcgt
     3721 tgccagcccg gcacaagcct cggttagcga tctgaccggt gatcagcctg cggcccagcc
     3781 ggtgcctgta agcgccccgg cgacaagcac cgcagcggta agcgcacccg caaatccatc
     3841 cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt
     3901 tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt
     3961 gctgaagagc aacaccccgc tgaacgtact ggcactgaac cagccggaga atatcgaaaa
     4021 ccgcgtcaat atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg
     4081 tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccacgca gtgcattggg
     4141 cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct
     4201 gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat
     4261 tgctttaacg ggtagcccga ttactcccag agcgacaacc gactccggca tgacgaccaa
     4321 caatccaacg ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt
     4381 cgatgcggtc tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc
     4441 catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg
     4501 caccgcaggc ccggatttcc gactggagat ggaaggcttg cagtacagcg aaatcccgat
     4561 gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga acaacgatta
     4621 ttcgctggct cgcatgtatg cgatgggcgt tgatgcctgg tcgttggcaa atcatttctc
     4681 acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagtc tgacggctaa
     4741 cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt
     4801 ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg
     4861 ctggaaggca aaggactgcg gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc
     4921 gatctgataa tgcgtgaagg cctgaccacc gtttttgtcg aggtacgcta tcgccgctct
     4981 gcgctttatg gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag
     5041 actgcccgct tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc
     5101 gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac
     5161 tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag
     5221 cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc gtgcagccat
     5281 gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc
     5341 cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc
     5401 cagcttacct gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga
     5461 tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt
     5521 attgttagcc atttccaccc gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc
     5581 cgttacgcgt gatatgacca ttgtggcatt gaccggctat gacggcggcg aacttgcagg
     5641 tttgttaggg ccacaggatg tggagatccg cattccttcg catcgtagtg ctcgcattca
     5701 ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc
     5761 tcaccaggat gattaaggag aatacatgaa ggcattatcg ccaatcgcag tccttatttc
     5821 cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac
     5881 caaagccgca actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt
     5941 gcgcgtgaac agcgcattgt cgaaagacga acagattaag aaagaaaccc gtattaatgt
     6001 cacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaatgctg aactttcggc
     6061 tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg
     6121 tcagggccag ccgattggtc tgggcgaagc atctaacgat acgtggatca ccaccaaagt
     6181 gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga
     6241 aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga
     6301 tattgccagc cgggtgagcg gcgtaaagcg ggttaccaca gcgtttacgt ttattaaata
     6361 gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct tatcaggcct acaacgatgc
     6421 aaaccgtagg tcggataagg cgtttacgcc gcatccgaca tgttttccct caaatcagaa
     6481 caacagcgcc agcccgccga caatcacacc agacactgcc accatcgccc ctgtcagcca
     6541 taaggctttc gccgggaacg ctttgcgcag cataatcagt gacggcaaac tcactgccgg
     6601 gagcgtcatc aacaatgcca gcgccggagc ggttcccata cctgccagca tcatcgtttg
     6661 tacaatcgga atttctgctg ccgtgggaat gacaaacaag catcctgcta ccgccatcgc
     6721 caccacccac atcaggctgt tatcgacagc accatcggca tgggggaata accagacgcg
     6781 tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg atcgtactcc agaaaagtgt
     6841 ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt gcttccggta tgtcaatttc
     6901 gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag
     6961 caacaccatc accagcccgg ccaccagacg aatcgccgca aaaccccagc cgaggacaaa
     7021 ccccataaac accagcgtcg ccgggtttaa caccggattg cccatccaga atgccagcgc
     7081 accgcccatc gatacctgtt ggcgacgcat tcccgccgcg accggagccg cacagcaggt
     7141 acacatcatg cccggcagcg aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg
     7201 cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat
     7261 cacccccagc accgccgctt tccatactgc gaggaaatag atcatcgcgt aatccaacgc
     7321 cgcttgccac ggattagcat ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc
     7381 ggctgcagta aacgctttgc cgtagtaagg ttgccatttc acataccaga gaccggcaat
     7441 gacaacgaga aagaaaagcg cgggtttcca ccactgaatg ggcgttgccg cctgagatga
     7501 agactgacca gtcatagcat tccccaggaa taagttatga taattgagcg cgtgaatatt
     7561 acgctcacta tcaattcttg ggaataatta ttcagctctt ttgcgtaatt ctgaagagct
     7621 taaaatcgtc acgccttcat gctcaggccg catcgcctct gccagcatga cccgcgccac
     7681 gtctctcgca tcaatggatt tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag
     7741 cgtttcgttc atccgctgtt tgctacgatc gcccagtaac atcgacgggc gagcaatggt
     7801 caatttcggc cagttctggg caattaatgc ttcttccatc tcccctttga cgcggttata
     7861 gaaaaacggc gagtgggcat tggcacccat agcactgacc accagcatat gctgcgcgcc
     7921 cagtcgccgc ccggttaatg cggtatccac taccagcgtg taatcggcat gaataaacgc
     7981 ctctttgctc cccgcttctc gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg
     8041 ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca tggggattaa acaccccggg
     8101 catatcgccc aacggacgtc gcgtcggcgc agcaatggcg ttaactttcg gttcattaat
     8161 caacatccgc agcaggtgac cgcccaccag ccccgttgcg cctgtaatca gtacctgact
     8221 catcttcgtt cctttacaga attgtctgcc ttgcgctccc cggctcatgc accagtctta
     8281 atagaccatg aggtaattat ccccgattgt ggaaaattcg cctcatccaa tgcaacaacg
     8341 cggaggaagc atgagtaaga aaattgccgt tttaatcact gatgaatttg aagattcaga
     8401 atttacttca cccgcagacg agttccgtaa agccggacac gaagtgatta ctattgaaaa
     8461 acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc
     8521 catcgatgaa gtgacgccag cggagtttga tgccctgttg ctaccgggtg gtcattcacc
     8581 ggattatctg cgtggcgaca accgttttgt cacctttacc cgtgattttg tgaatagcgg
     8641 caaacctgtt ttcgccatct gtcacggtcc gcagctgctg atcagcgccg atgtgattcg
     8701 ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat gttaaaaatg cgggcgtgga
     8761 attttacgat caggaagtcg tggttgataa agatcagctg gttaccagcc ggacaccgga
     8821 cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc
     8881 aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga
     8941 tttcccacac cggggccgac agcattctgg caaccggaaa gcgacgattg tacgccttgc
     9001 gcgcgaggtc gctttcctca ccttccagcc tgcggatctc acctttaaac tgcacaccgc
     9061 gaattaacgc taccgttttc ggctgaccgt ttaccgttcc ggcaaccgca gcctgcggcc
     9121 cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat gtagaaggcg actttctgcg
     9181 catcaaaaag ataaaaggca ttagcgcacc agagttcccc ttcctgctgc acacaccagg
     9241 tgacaacatg ttgttttgcc agccaacggc tgatggcgat gagtgtttcc attgctgttc
     9301 tcttttatac tgtgggcctg accttaacat gcgatgcaga cgatgacacc ctggtttctt
     9361 tacttgatcc gtaccgccga caataagctt tataccggga tcaccacgga tgtcgaacgc
     9421 cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac tgcgcgggaa aggagaactg
     9481 acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg cactacgggc ggaatatcgc
     9541 gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag cggaaggcgc agggtttgca
     9601 gaactgttga gcagcctgca aaccccggag attaaaagcg attgaaatgc tcgtgatact
     9661 caaccagacc ggttacgcca ttcagcgcgt catccgctaa acgatgtacc aggaaggcgc
     9721 tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc cgccagttca aagccgaaac
     9781 gactgtacag cgccggatcg cccagcgtca ccactgcggc atagccgaac tcattaagcg
     9841 aatcgagtcc ttcatagacc agttggcgtg ccagcccctg tccacggtat ttttcatcaa
     9901 ccgccaacgg tgccatgccg acccattgca ggtcttcgcc ctgcacatca accggactaa
     9961 atgccacata gccaatgacc tgaccttcgt catctgtcgc caccagcccc agcgtcagaa
    10021 agccatcttc acgcagatcg tgaaccagct tcgcttccgc atcactttcg aatgagcgac
    10081 gcagcagggc atcaataccc ggcgcatcaa tgggaatttc tactcgaatt agcatggttc
    10141 acctaccgat gtctgtttgg tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc
    10201 cagttgcagc agcatcatgc gtaaggcttt cggcatttgc tccagttcaa tggcgtccat
    10261 caggtttttc acatacaccc ccagctccgt atcgccttca atcaccagcc gacgctggaa
    10321 gaagagcgta tccggatctt gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc
    10381 actaaaactc acatcagctt gcgtgttctg gctgaccacc agtttgccat tcaccaccga
    10441 ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt aaccagcggc cttcaagaaa
    10501 ctccagctcg ccatcatcca gcgcctgacg gaattgccag cttaagacct gctcaagaac
    10561 ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca ctcaacagag atggccccaa
    10621 atgcacaata cgggaacgca gtttatccaa cacgagtttt actccctgtt tcaacaatca
    10681 tcctattttg ccatatcaga aaaataacat agcggtataa atcaacaatt ccatatgaaa
    10741 ttgctgctac caccaataca actttaactg ccttaaatca aaaattgtcg cagcaaggtt
    10801 aactaaaatc ccagttcgtt aacatttttg cgttttgata gcgcaacctt caggaaaaat
    10861 tatggagctg ctctgccctg ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg
    10921 cgcagatgct gtttatatcg ggctaaaaga tgataccaat gcccgtcact tcgccggcct
    10981 taactttacc gagaaaaaat tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa
    11041 acttcacatc gcgattaaca cttttgcgca tccggacggt tacgcccgtt ggcagcgtgc
    11101 cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct
    11161 ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat gtgtcagtgc aggcttcggc
    11221 gaccaatgaa gaggcgatta acttttatca tcgccatttt gacgttgccc gcgtggtgct
    11281 gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca cgggttactc ctgtcccgct
    11341 ggaagtgttt gctttcggca gcctgtgtat tatgtcggaa ggccgttgct atctgtcgtc
    11401 gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt tctccggccc gtttcgtgcg
    11461 ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta
    11521 tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa gggcgttatc tggtggacgg
    11581 cgagcgctat cacgcgctgg aagaaccaac cagtctcaat accctggaac tgctgccgga
    11641 gttaatggct gcgaatattg cttcggtgaa aattgaaggc cgccagcgta gcccggcgta
    11701 tgtcagccag gcggcgaaag tctggcgtca ggctatcgac cgttgtaagg ccgatccgca
    11761 aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg aaggcacgca
    11821 aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa atattcctta
    11881 gggccagtgc tgtggtactg gccaaaagag acgctggaag atttttatca gcaagccgcc
    11941 gccagcagcg ccgacgtgat ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc
    12001 aaagttggcg actgtctgga gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg
    12061 ctctccacgc tggcgctggt gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt
    12121 gagaacggtg aatttctgat tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa
    12181 cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa
    12241 atattgctca aacagggcat gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg
    12301 ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa
    12361 gtcctgagct acggtcatct accgctggcc tactccgccc gctgctttac cgcgcgttcg
    12421 gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac
    12481 gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg gcattcagac catgagcggc
    12541 tacgtttaca acctcggtaa cgagctggca tccatgcagg gattggttga tgtagtccgc
    12601 ctgtcaccgc agggcactga cactttcgcg atgctcgacg ccttccgcgc taatgaaaat
    12661 ggcactgtgc cactgccgtt gacggcaaac agcgactgta acggctactg gcggcggctg
    12721 gcaggactgg agctgcaagc ctaagtaaat agctcacttt gttaacaact ttaactattc
    12781 tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact gataaaacca
    12841 ttgcgttttc gctactcgat ctggccccca tacccgaagg ctcttcagcg cgggaagcgt
    12901 tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat cgctactggc
    12961 tggcagaaca ccacaatatg accggcattg ccagtgctgc aacatcggta ttgattggct
    13021 atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc
    13081 acttaccgtt ggtcattgcc gaacagttcg gcacactcaa tacgctctat ccggggcgaa
    13141 tcgatttggg gctgggtcgt gctccgggta gtgaccaacg gacaatgatg gcgctacgtc
    13201 gtcatatgag cggcgatatt gataatttcc cccgcgatgt cgcggagctg gtggactggt
    13261 ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctatggc gagaaaatcc
    13321 ccgtgtggtt gttaggctcc agcctttaca gtgcacaact ggcggcgcag cttggtctgc
    13381 catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg catctttatc
    13441 gcagtaactt caaaccgtcg gctcggctgg aaaaaccata cgcgatggtg tgcatcaata
    13501 ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt tacctcaatg cagcaagcct
    13561 ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc gccgattcaa aatatggatc
    13621 agttctggtc accgtctgag cagtatgacg tgcagcaggc gctgagtatg tcgttggtag
    13681 gcgataaagc gaaagtgcgt catggtttgc agtcgatcct gcgcgaaacc gacgctgatg
    13741 agattatggt taacgggcag attttcgacc accaggcgcg cctgcattcg tttgagctgg
    13801 cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa gccttatccg
    13861 agctggcaac gctgtcctac gtaggcctga taagcgaagc gcatcaggca ttgtgtaggc
    13921 agcagaaatg tcggataagg caccgctgat tactgataca ccggcagtaa attaaagctc
    13981 gataaaatat gcaccagtgc gttgccgacg ccaaacacca gaatcagcgc aatcatcggc
    14041 ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt tacgcgatgc gcgggccagc
    14101 agtgccggaa caattgccgc ccagatggta gccgctaaac cagcataacc aatggcatac
    14161 aggaatccgt tcgggaacag cagaccaccc acaacaggcg gggcaaaggt cagcaatgcc
    14221 gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca gatctgccag atagtcaaac
    14281 aaacccagcg ttacgccaag gaacgaactc gctaccgcaa agtttgagaa cacgaccagc
    14341 agaaggtcca ggctacggct gttcagcacg ccgcttaatg cttgtaccaa cacatcaata
    14401 ttaccgccct tctcggcgat gccgataaac tccggacgag ggatgttgcc cattgtcgcc
    14461 agcaaccaga tggtatacag cgccagtgcc atcagcgtac cgtacaccag gcatttcacg
    14521 atggttttcg gatctttgcc gtaatacttc atcaggcttg gcacgttacc gtgataacca
    14581 aacgacgcca ggcagaacgg cagggtcatc agcaaatacg gcgcataaga tgcattgctt
    14641 tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca gcaggctacc gaaggtgagg
    14701 aagaaggtaa tgactttcgc ccccagcaca atcgccgtca tgcggctgac ggctttagta
    14761 ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc ccgtgccggg
    14821 acgtttagcg acatctctgc gaaggtgtga tgcagaatcg aaccactggc agaaatatag
    14881 gcataggtca ggatatagag cacaaaggca atggaaatgc cgttgaccac gttccagcct
    14941 ttgcccagca aatctttggt gatggtgtca aaactcgaac cgattcggta attcaggtta
    15001 gcttccagaa tcatcaagcc ggaatgcagc atacagaacc aggtaaagat cagcgctgcc
    15061 attgaccaga aaaaccacgc cccggacatg accactggca gagaaaacat ccctgcgcca
    15121 ataatggtgc cgccgataat caccacgccg ccaagcagcg acggtgacgt ttgggtggtg
    15181 gttagtgttg ccatgagggc ttctctccag tgaaaaatag tgcgactgcg ttgttatgca
    15241 ttgcactgta ccagtacatg agtacaaaag acagaaaaaa agccccgatg gttaaaatcg
    15301 gggctgtata tattatttta cagattgtgt tcgctgttca gcgatgatta cgcatcacca
    15361 ccgaaacgac gacgaccggt agagtcatca cgacgcggtg cgcggccttc acgacgttcg
    15421 ccgctaaaac gacgaccatc accacgacca ccttcacggc gttcaccgct gaagttacga
    15481 ccgccttcac gacgctcgcc accgaaacca cgaccaccgc cacgacgctc accgccagta
    15541 tgcggctgtg catcgcccag taactgcatg ttcatcggct tgttgagaat gcgagtgcgc
    15601 gtaaagtgtt gcagcacttc acccggcata cctttcggca gttcgatggt ggagtgagaa
    15661 gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt cgccttcgtt agcaatcgcg
    15721 ccaacgatat gacgaacttc aacaccatca tcgcggccca cttcaatgcg gtacagctgc
    15781 atatcgccaa catcacgacg ttcacgacgc ggacgatctt cacggtcgcc acgcgggcca
    15841 cggtcgttac gatcgcgcgg accacggtca tcacggtcac ggaattcacg tttcggacgc
    15901 atcggcgcat ctggcggaac aatcagagta cgttcaccct gtgccatttt cagcagtgcc
    15961 gcagccagag tttcgagatc cagctcttca ccttctgcag tcggctgaat tttgctcagc
    16021 agtgcgcggt attgatccag atcgctgctt tccagctgct gctgtacttt agcggcgaat
    16081 ttttccagac ggcgtttgcc cagcagttct gcgttcggca gttctacttc cggaatagtc
    16141 agcttcatag tacgttcaat gttgcgcagc agacgacgct cgcggttctc aacgaacagc
    16201 agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga tacggtgaac gtaagactca
    16261 gaatccatcg ggatatcgta gttaaccacc aggctgatac gctcaacgtc caggccacgg
    16321 gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt ctttaaggcg ttccagtgtc
    16381 tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg cgctattgta gccgttacgc
    16441 tcaagagctt cagccacttc cagagtcgcg tttttggtac gaacgaagat aatcgccgca
    16501 tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt ttttacgcat accccacaca
    16561 gtccagtagc tctggctgat gtcaggacgg gtagtcacgc tggactgaat acgcacttcc
    16621 tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg cttccggcat ggttgcagag
    16681 aacagagcgg tctgatgacc ttccgggatc tgcgccataa tggtttcaac gtcttcgatg
    16741 aagcccatgc gcagcatttc gtcagcttca tccagaacca gaccgctcag tttagagagg
    16801 tccagagtgc cacgtttcag gtggtccagc agacggcccg gagtaccgac aacgatctgc
    16861 ggcccctgac gcagggcgcg taattgcacg tcataacgct ggccgccgta cagggcaacc
    16921 acatttacgc cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc
    16981 agttcgcggg tcggtgccag caccagaatc tgtggtgctt tcagctcagg atcaagattc
    17041 tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc catacccaga
    17101 acgtcgcggc cattcagcag atgtggaata cactctgcct gaattggaga tggtttttcg
    17161 taacccagat cgttaagggc ttcaaggata ggagccttca ggcccagatc tgcaaaagtg
    17221 gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta atggcggcca gtctacataa
    17281 ctcatcatga aattgatcag caattttcat tgaaaagtgt gaaccggctc aaagtaggtg
    17341 tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg caatcaaaaa agattacggg
    17401 ctgatgtgta cgtcagctat tgctggtccg attctgccag gtcatcttgg tcctggccca
    17461 ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa
    17521 ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc cagacttagg tagtacttac
    17581 ctaaatagaa gttggtttca ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg
    17641 ccttgagcct ttccattaac gtttgttcgc taatgttgcc caggtagaac tcgacaatgt
    17701 tccatcccca ctgttcctta tccgattttt cgaagtgctg tttcaatact tctttagcct
    17761 gcttctcatc gagcttctgc tcggcgagat aaagccacag actacggaaa ggatcattgg
    17821 gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa cttgtcacga ccgccgtaat
    17881 ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag
    17941 aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt
    18001 aattgaatac ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg
    18061 ctaatgccct cagaccgaga ctatcataca acactccgcg ctcatataaa agctgtgcgc
    18121 gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc catacgtgcc agaatcactt
    18181 cctgctgtaa agtcggttgc aatggtaccg cgaggacttc acttttacgc caggaagtat
    18241 tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca
    18301 tttcccactc ccgaagacca cggttgaatg aacgtcctgt tcccggttgc taacaaggcg
    18361 tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa atggcaacct tactcgccct
    18421 gttcagcagc cggagcttcc ggtgctgcag caggttgaga ctgctcagtc gcttctttaa
    18481 tgctcagacg gatacggccc tggcgatcaa cttccagaac tttcaccggt acttcctgac
    18541 ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc agcgatttga gagatgtgta
    18601 ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca
    18661 ctttaccagt gtagacgcgg cccacttcga tttctgcagt gatctcttcg atacgacgaa
    18721 tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt cacagtaccg tcatcttcga
    18781 tttcgatggt ggtgccagtt tcttcggtca gggcacggat aacagagccg cctttaccga
    18841 taacgtcttt gatcttgtcc gggttgatct tgatggtatg gatacgtggt gcgaactcag
    18901 agatatcgcc acgcggcgcg ttgatcgcct gttccattac gcccaggata tgcagacgcg
    18961 cacctttagc ctggttcagc gcaacctgca tgatctcttt ggtgatacct tcaattttga
    19021 tatccatctg caatgcagag ataccctcgc gggaacccgc aactttgaag tccatatcgc
    19081 ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg ccttctttca
    19141 ccagacccat tgcgataccc gcaacggcag ctttgatcgg cacacctgcg tccatcagcg
    19201 ccagagacgc gccgcacacg gaagccatag aagaggaacc gttggattca gtgatttcag
    19261 acacaacacg tacggtgtac gggaatttgt ccatatccgg catgactgcc agcacgccgc
    19321 gcttcgccag acgaccgtga ccaatttcac gacgcttcgg agaaccgacc atgccggttt
    19381 cgcctacgga gtacggaggg aagttgtagt ggaacaggaa ggtatcggta cgttcgccca
    19441 tcagttcatc aagaacctgc gcgtcacgag cagtacccag cgttgcggta accagcgcct
    19501 gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg cggcagcacg ccagtacgca
    19561 catccagacc acggatcata tctttttcac gaccgtcgat acgcggttcg cctgccagta
    19621 cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat ttcacccagt tcgttttcgt
    19681 ccagggtttc gtcttcagca agcagcgtcg cgatggtttc agatttgatg acatcaacct
    19741 gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca
    19801 gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag tcccaacgtg
    19861 gtttaccggc ttctttcacc agttcattga tgttctgaat aacaacctgc tgttgttcat
    19921 gaccgaacac tactgcgccc agcatctggt cttcgctcag cagttcagct tcagattcaa
    19981 ccatcagtac agcggcttca gtaccggcaa caaccagatc cagtttgctc tctttcaact
    20041 cgtcctgagt cgggttcagt acgtactggt cattgatgta acctacgcga gcagcaccaa
    20101 tcggaccatt gaacggaata ccagacagag acagtgctgc ggaagcacca atcatcgcga
    20161 cgatatccgg gttaacttgc gggttaacag aaaccacggt ggcgataacc tgaacttcgt
    20221 tgacgaagcc ttccgggaac agcggccgaa tcgggcggtc aatcagacgc gcgatcaggg
    20281 tttcgccttc gcttgggcgg ccttcacgac ggaagaagct acccgggata cgaccagcag
    20341 cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa gtcctgacct ggtttggctt
    20401 ttttctggcc aacaacggta acaaataccg cggtgtcatc catgctaacc ataacagcgg
    20461 cagtagcctg acgagccatc atgccggttt ccagagtcac ggtgtgttgg ccgtactgga
    20521 atttacgaac gatcggatta agcaatgtaa tatcctttct ctttcttaga cagtacctta
    20581 cggcactggt gttaataccc gatcttctgc gcatcctcgc gactaatgac aaccctaacc
    20641 cagctctatg tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt
    20701 catagcaaca atacattagt ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca
    20761 ctcaggcccc cttttctgaa actcgcaaga attagcgacg cagacccagg cgctcgatga
    20821 gctgggtgta acgtgctacg tctttacgtt tcaggtagtc gagcagttta cgacgctgag
    20881 aaaccatgcg cagcagacca cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc
    20941 cctgcaggtg gttgatctgt gcagtcagca gtgctacctg aacttcagta gaaccggtgt
    21001 cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt tgcttcagta cttagagaca
    21061 ttttaaaact ccaaagtata tagaatgaaa ggacgccgat ctctaattca gcgatcccag
    21121 tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta agcggcagta ttctactcgt
    21181 agcgacctgt tatcgcaaga cggttaacat tacgccgggt attcaaccac caggcgacga
    21241 ggcgcaacgc ggccttcatc gtcaatttcg cccataccga taaatttgcc gttctcacct
    21301 tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg tacgaactgg gttaccattt
    21361 ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg ggtagtccga agctggactg
    21421 tccattggca tcagtaatgg atcaagtaac tccgcagccg gaatatcctg ctgttcggct
    21481 tgttcgacca gttcacgcag atgttccagg gtcacaatcc gttcaaccgg atatttactt
    21541 accgccagac gacgcaggta aataacatgc gcgccacaac cgagtttttc acccaggtca
    21601 tcaatgatgg tgcggatata agtgcctttt gagcagtgaa tttccagctc cagctcattg
    21661 ccttcgtggc gaataaacag caattcataa acggtaatcg ggcgcgcttc acgcggtact
    21721 tcaatgccct gacgcgcata ttcgtacagt tttttaccct gatatttcag tgctgaatac
    21781 atcgaaggga tctgttcgat atcgccacgg aaagtatcca gcgccgccgc cagttgctct
    21841 gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt cggcatcaga agtatcggta
    21901 cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg agtccagcag atactgggaa
    21961 aacttcgtcg cttccccgag gcaaattggc aacatgccag ttgccagcgg gtccagcgca
    22021 ccggtatgcc cggcacggtt ggcgttataa atccgtttca ctttttgcag cgcatcattg
    22081 ctggacatac cctgcggttt atccagcaac aaaacgccgt taatgtcgcg accgcgacga
    22141 cgaggacgac tcattagtcc tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat
    22201 gtttgaccac gctggtcacc aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt
    22261 agaagaaggt cagttccggc acgatacgca ggcgcatcgc tttccccagc aggctgcgga
    22321 tgaaaccaga agcttcttgc aacgctttga tgcccgcttt aaccgcgtct tcatctttgt
    22381 cgttgaggaa cgtcacatat actttggcat acgccaggtc gcgagacatt tcgacaccgg
    22441 aaacggtggt catcatgccc aggcgaggat ctttaatttc acgctgcagg atgagagcga
    22501 tctctttttg catttcctgc gctacgcgct gcgggcgacc aaattctttc gccataataa
    22561 attctcctga caaaaaaggg gctgttagcc cctttttaaa attaatttca ggtggaaggg
    22621 ctgttcacgt tggcctgata agacgcgcca gcgtcacatc aggcaatcca tgccggatgc
    22681 ggcgtaaacg ccttatcccg catggaaccc taaaaacctt aagcgatggt acgttggatc
    22741 tcgatgattt cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg
    22801 ccgataccac attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg
    22861 gactccagct cgccttcgta gataaccacg ttgtcacgca gaacgcggat cgggttgtga
    22921 cgtttaacca caccttcggt aaccatacag cctgcgatgg caccaaattt cggcgatttg
    22981 aacacgtcac gaacttccgc cagaccgata atctgctgtt tcagttccgg agacagcata
    23041 ccgctcatcg ccgctttcac ttcgtcaatc aggttataga tgacggagta gtaacgcaga
    23101 tccaggcttt ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca
    23161 accaggatgg cgttggacgc cgcagccagg gtggcgtcgg tttcggtgat accacctacg
    23221 ccagaaccga tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag
    23281 atcgcttcca cagaaccctg tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg
    23341 ccttcggtca tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta
    23401 acttcgcgga atttaccctg acgatacagt gcaacttcac gcgctttctt ctcgtcacgt
    23461 acaacggtaa cttcatcacc cgcagccggt acgccggaca ggccgaggat ttccaccgga
    23521 atggacggac ccgcttccag cacttcctga cccagttcgt tacgcatcgc acgaacacga
    23581 ccgtattcga agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga
    23641 acggtagcaa ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc
    23701 atacctttac gtaccgcttt cagctccaga acttccgcct gcagcaggat agcgtccagc
    23761 agttcatcga taccagtacc cgctttcgca gacacatgta cgaactggct ttcaccgccc
    23821 cactcttccg gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct
    23881 tctggtttat cgatcttgtt cactgcaacg accaccggaa cctgcgccgc tttcgcgtgc
    23941 tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg cagcaacaac caggactacg
    24001 atgtccgttg cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg ccccggggtg
    24061 tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta
    24121 atgccgcccg cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt
    24181 ttaccgtggt caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgcagca
    24241 cccgtgtcac ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact
    24301 ttatggccca tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg
    24361 gttgccattg cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc
    24421 ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca caacgtcacg gttaacggcc
    24481 tgagcaggct tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcctttaccg
    24541 ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttgcct
    24601 ttcttcggac gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga
    24661 tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa
    24721 tcttcagtcg gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct
    24781 tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc agctttacgc
    24841 ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata
    24901 tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca
    24961 gcttcacgct tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta
    25021 gccgattctt ctgcctcacg acgggcttgc tcttccgctg caaggcgttc agcctcttgc
    25081 ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt
    25141 ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc
    25201 gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca
    25261 gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg
    25321 gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct
    25381 gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt
    25441 gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg
    25501 gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct
    25561 accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg
    25621 gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc
    25681 tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc
    25741 gtcgagaaac cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg
    25801 gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg
    25861 gtcattacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta
    25921 cggccaatcg cctgcgccag gttaccagct tcaacggcga tatccatggt gtgtttatct
    25981 tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc
    26041 gggttatcat cccacaggac gatatcgata cgctcgccgc ccagttcagt agaaaccgcc
    26101 tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg
    26161 gttttcaccg cgattttcgc acgagaaccc gggtcgcgag ccgctgcttt aatttcaatc
    26221 acttcttcgc cgatttccgg cacttcaata cggaacagtt cgatcagcat ttccggcttg
    26281 gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata aagcacgcca
    26341 cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg caggatcacg
    26401 gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg
    26461 ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct
    26521 tcacgcactt tctgcacgat aacctgtttt gccgtctggg tggtgatacg gtcaaaggta
    26581 acagactcaa tctgatcttc aacgtaatcg cccaagttca ggctttcatc ttcataacgt
    26641 gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga
    26701 cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cacggacgtc gatctcttgc
    26761 tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg
    26821 cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc
    26881 gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata
    26941 ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct
    27001 accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga
    27061 gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga
    27121 cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca
    27181 gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca
    27241 atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgcctac cagttcaaaa
    27301 cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag
    27361 cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa
    27421 aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg
    27481 agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata
    27541 tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc gacgaccttc
    27601 gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac gtggcaaatt
    27661 ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg agacgggacg
    27721 taaaatctgc aggcattata gtgatccatg ccacattttg tcaacgttta ttgctaatca
    27781 cgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttcc cctgctctcc
    27841 cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga taaaagatga
    27901 ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcggaaat ccaggctcat
    27961 catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat ctcccggtag
    28021 gtcaacgtat tggtatcgct ttttccggcg gtctggacac cagtgccgca ctgctgtgga
    28081 tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa cctgggccag ccagacgaag
    28141 aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga
    28201 tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt ggcgcatttc
    28261 ataacaccac cggcggcctg acctatttca acacgacgcc gctgggccgc gccgtgactg
    28321 gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa tatctggggt gacggtagca
    28381 cctataaagg aaacgatatc gaacgtttct atcgttatgg tctgctgacc aatgctgaac
    28441 tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc ggccgtcatg
    28501 agatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct
    28561 actccacaga ctccaacatg cttggtgcaa cgcatgaagc gaaggatctg gaatacctca
    28621 actccagcgt caaaatcgtc aacccgatta tgggcgtgaa attctgggat gagagcgtga
    28681 agatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg gcgctgaacg
    28741 gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc
    28801 acggcctggg catgagcgac cagattgaga accgtatcat cgaagcgaaa agccgtggta
    28861 tttacgaagc tccggggatg gcactgctgc acattgcgta tgaacgtctg ttgaccggta
    28921 ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg ggccgtctgc
    28981 tgtaccaggg gcgttggttt gactcccagg cgctgatgct gcgtgactct ctgcaacgct
    29041 gggttgccag ccagatcact ggtgaagtta ccctggagct gcgccgtggg aacgattatt
    29101 caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg acgatggaaa
    29161 aaggcgactc ggtgttctcg ccagatgatc gtattggtca attgaccatg cgtaatctgg
    29221 atatcactga tacccgagag aaactttttg gttatgccaa aactggcctg ctttcctcct
    29281 ctgccacttc aggcctgccg caggtggaga atatggaaaa caaaggccag taattcgaca
    29341 tcaaccctgc ccttcagggg tggggttacc ttgcttatcc tctgtaaact cctttctgag
    29401 catcacataa tcgaaaacat catggtttgc atccacaacc cgtgagtctc ctttatagtg
    29461 tgcaatcact tcctcaagcg tttgcggctg ttccggcttt gttaccccca tccacgcatt
    29521 aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt tccgttgtgc gatccacgcc
    29581 atccccaaga acggggctat accagataaa catcgggaca tgatatgcct gctggctagc
    29641 ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcacgttcca ggccgtggtc
    29701 ggcaaaatac atgaccgagg cgcggcgatc ttttaataat tcaaaaacct gacctagcaa
    29761 actatctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct gcggttgaaa
    29821 cacggcgctg gattgcggat aggcgctaca agccggttca tggcttccat ttaaatgaag
    29881 aacaatcagt ttcttttgct gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa
    29941 caattcatca aatcctctga cataaactgt ttccatggcg cgcatggcga tactggtaac
    30001 tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct gaaatcctgc
    30061 ttgattagcc atattaataa tgttgtcggg gtaattatga atgtcatgac tcaaaacaga
    30121 atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg
    30181 attaaacagt ttgatctgtt ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata
    30241 tccatacaaa gacatattgt cgacacgtac agactccccc acaatcaaca cgtaggtatc
    30301 aatacttgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa
    30361 tctttgatgc gctttcgctg ctaaagcaaa atagttgaga ttgaaaaacg gcgtataggt
    30421 agcaaatcac gacgctagta tatatggact gaacgctttt ttattttttg catctttata
    30481 agcaaattga caagcggaaa ataaactgcc cgaaatgaca atcagcaata atattcctgt
    30541 cacttttttt gtcggcaagg aaacatcata ttttattatt actgccaaaa aaagaaggga
    30601 taaaaaagca aaagcacata gataagggat atacatcccc agcattttca caacttcatc
    30661 cggatcactc tgcagcacac taatcgcaaa tccatcattg aaagttgtac caaaggtaca
    30721 ccatgaatag aggctaatac tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac
    30781 aagcaggcga agcggaaggg atgatatacg tttaacaacc aggatggtca ggacgaagaa
    30841 cagcaatcgc tgaaccatat gcgctccgga agaggcgact aagttcgtaa taccaaaaac
    30901 aataacacaa agatacaaca accaatgaga tttaaaatct tctagcaaat ttgttgaata
    30961 ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgttcaattt
    31021 tatatcagac atgtttttca actctgatcc atatcagacc gaaacactgt tagttttttc
    31081 tttttaaatg atgcgaccat tgcgttctca gtaattaaat aacattatca atgcgtctct
    31141 aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg
    31201 agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta
    31261 tgtcagcagt agggaaaagt ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc
    31321 agctacgagt aaagcaactg gacgagatac agatacctga caaccattca tcagaccagg
    31381 accaaaacga aaagagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg
    31441 gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc
    31501 caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga
    31561 gcagctggct gagtttgttc ggttttcgcc ggtgcactca gattttccca ttcgctacct
    31621 ctattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt
    31681 gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta
    31741 gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg
    31801 atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc ttcgtacata
    31861 atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt
    31921 tttaatcttt cccactgaag cgggtgtgaa tactaaccaa aacgaatgac cttcgcaagg
    31981 gcaattttat cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgccga
    32041 gaaaaaaggc gacaatcgcc gcctttttag ccagttatct aacgctttaa acggctttta
    32101 ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca
    32161 tcacgcgaat taacggttcg gtgccggatt tacgcagcaa cacgcgtcca cggttgccca
    32221 gcgcagcttc aacctctgcg gtcacggctt taactgactc atgctcaagc ggatcgccgc
    32281 tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa
    32341 ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag cacctgcaag ccagcaacga
    32401 tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggaattc tctgcaccga
    32461 tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg
    32521 caaatggaat tcctaactgt ttcagcgcca gttcaagccc catgttgctc atcaatgtac
    32581 ccacagcacc accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga
    32641 tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc
    32701 cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa
    32761 cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt tggctcacaa ccgatagcga
    32821 taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg
    32881 cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac gtggctttgc
    32941 aaaactcgat atagcgaccc gcggcatcaa cgatacggct ggctttaccc agttcagccg
    33001 aatcaacgca gctgatctcc ttttccattt ccgcttcgat ggcctcttct accgcatccg
    33061 gcagtttagt gccgtcgata gagaagaatt taatcccatt atcgtagaac gggttatgcg
    33121 atgcagatat cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg
    33181 gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca
    33241 gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg
    33301 agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcaggtg
    33361 tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac
    33421 tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg
    33481 gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca
    33541 caggccagac taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc
    33601 gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc
    33661 gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac
    33721 aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga
    33781 ttcacttctg caaagacatc gtcatacttc ggagcttcct gcatggtttt tggatttccc
    33841 tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag
//
LOCUS       DAJTRW010000039        33535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRW010000039 DAJTRW010000000
VERSION     DAJTRW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO326_001986"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO326_001986"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5966378.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO326_001987"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO326_001987"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5966379.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO326_001988"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO326_001988"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5966380.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO326_001989"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO326_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5966381.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO326_001990"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO326_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5966382.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO326_001991"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO326_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5966383.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO326_001992"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO326_001992"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5966384.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO326_001993"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO326_001993"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5966385.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO326_001994"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO326_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5966386.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO326_001995"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO326_001995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5966387.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO326_001996"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO326_001996"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5966388.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO326_001997"
     CDS             8212..9075
                     /locus_tag="OO326_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5966389.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO326_001998"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO326_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5966390.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO326_001999"
     CDS             10411..11028
                     /locus_tag="OO326_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5966391.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO326_002000"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO326_002000"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5966392.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO326_002001"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO326_002001"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5966393.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO326_002002"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO326_002002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5966394.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO326_002003"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO326_002003"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5966395.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO326_002004"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO326_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5966396.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO326_002005"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO326_002005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5966397.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO326_002006"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO326_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5966398.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO326_002007"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO326_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5966399.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO326_002008"
     CDS             21859..23568
                     /locus_tag="OO326_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5966400.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO326_002009"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO326_002009"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5966401.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO326_002010"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO326_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO326_002011"
     tRNA            27625..27719
                     /locus_tag="OO326_002011"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO326_002012"
     CDS             27991..29202
                     /locus_tag="OO326_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5966402.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO326_002013"
     CDS             29614..30657
                     /locus_tag="OO326_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5966403.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="OO326_002014"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="OO326_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="OO326_002015"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="OO326_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32846..33494
                     /locus_tag="OO326_002016"
                     /pseudo
     CDS             32846..33494
                     /locus_tag="OO326_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8402 a         8816 c         8263 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccgg
//
LOCUS       DAJTRW010000040        33447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRW010000040 DAJTRW010000000
VERSION     DAJTRW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO326_002017"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO326_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5966404.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO326_002018"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO326_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5966405.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO326_002019"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO326_002019"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5966406.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO326_002020"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO326_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5966407.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO326_002021"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO326_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5966408.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO326_002022"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO326_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5966409.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO326_002023"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO326_002023"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5966410.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO326_002024"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO326_002024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5966411.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO326_002025"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO326_002025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5966412.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO326_002026"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO326_002026"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5966413.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO326_002027"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO326_002027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5966414.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO326_002028"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO326_002028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5966415.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO326_002029"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO326_002029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5966416.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO326_002030"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO326_002030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5966417.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO326_002031"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO326_002031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5966418.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO326_002032"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO326_002032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5966419.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO326_002033"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO326_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5966420.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO326_002034"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO326_002034"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5966421.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO326_002035"
     CDS             19508..20329
                     /locus_tag="OO326_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5966422.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO326_002036"
     CDS             20316..22424
                     /locus_tag="OO326_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5966423.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO326_002037"
     CDS             22421..24088
                     /locus_tag="OO326_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5966424.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO326_002038"
     CDS             24091..25617
                     /locus_tag="OO326_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5966425.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="OO326_002039"
     CDS             25618..27234
                     /locus_tag="OO326_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5966426.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO326_002040"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO326_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO326_002041"
     CDS             complement(27465..27842)
                     /locus_tag="OO326_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966427.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO326_002042"
     CDS             complement(28252..28623)
                     /locus_tag="OO326_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966428.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO326_002043"
     CDS             complement(28684..29181)
                     /locus_tag="OO326_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966429.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO326_002044"
     CDS             complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO326_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5966430.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO326_002045"
     CDS             complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO326_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5966431.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30722..31195)
                     /locus_tag="OO326_002046"
     CDS             complement(30722..31195)
                     /locus_tag="OO326_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5966432.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31527..32063
                     /locus_tag="OO326_002047"
     CDS             31527..32063
                     /locus_tag="OO326_002047"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5966433.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32178..32504
                     /locus_tag="OO326_002048"
     CDS             32178..32504
                     /locus_tag="OO326_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5966434.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32692..32931)
                     /locus_tag="OO326_002049"
     CDS             complement(32692..32931)
                     /locus_tag="OO326_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5966435.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8674 a         7805 c         8447 g         8521 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
    29401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    29461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
    29521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    29581 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
    29641 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
    29701 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
    29761 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
    29821 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
    29881 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
    29941 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
    30001 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
    30061 aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc
    30121 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
    30181 cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg
    30241 tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt
    30301 tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac
    30361 aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag
    30421 ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat
    30481 gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag
    30541 gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac
    30601 ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa
    30661 acctggcgcg gcttttggta caaaaggcgt gacaggtttg cgaatccgtt gctgccactt
    30721 gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag tcttgggtaa
    30781 aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc ggctcgatgt
    30841 ctattgtaga tatatgtagt gtatctactt gatcgatcag gcttttgtat atctccccac
    30901 cacctgaaac aatgacatga tccgttattt tctttaggtt ggttaaagca tctttaattg
    30961 atggaaagat caatacgttc tcattgtcag atgtaaaact tgaacgtgtt acgaccgcat
    31021 actttcggtt gggtaatgct cccattgatt caaaagtctt gcgtccaacc aacagccatt
    31081 ggttataggt aatagcttta aacaggagct gttcaccttt ggcactccat ggaatatcag
    31141 ggccattccc gataactcca ttcttcgata tagctaccat tagtgatagt ttcacaattc
    31201 ttcctcagag gttaacattt tattagtaag catgctcgtt ttgtcacccg ctgatgctta
    31261 ccgttaatta atataatcac ttgtattaaa tagactttct ttaaaaatac aagacactct
    31321 gttattacaa atcgtgcatg ccgtctatcc tgcttgcacg atgcacatta tctcacccaa
    31381 agaactcata acattttaat ttatttagca ttaaattaaa actctaccgg taaacaagca
    31441 tctctaggcg taaaaaacag gcactttttt aaatcccagc tagaataggc tgtataggca
    31501 gacagttgca agacaaggag cgttttatgt ctaacagtcc atttttaaat tctatacgca
    31561 cggatatgcg acaaaaaggt tatgcgctga aaactgaaaa aacttacctg cactggatta
    31621 agcgttttat tctgtttcac aaaaaacgtc atcctcagac catgggcagt gaagaggtca
    31681 ggctgttttt atccagctta gcaaacagca gacatgtagc cataaacacg cagaaaatcg
    31741 ctttaaatgc cctagctttt ttgtacaaca ggtttttaca acagccgttg ggcgatattg
    31801 attatatccc tgcaagcaag cctagacggc taccctctgt tatctctgca aatgaagtgc
    31861 aacgcatttt gcaggttatg gatactcgca accaagttat ttttacgctg ctgtatggtg
    31921 caggtttgcg cattaatgaa tgcttgcgtt tgcgggttaa agattttgat tttgataatg
    31981 gctgcatcac tgtgcatgac ggtaagggtg ggaaaagcag aaacagccta ctgcccacgc
    32041 gcctaatccc agcaataaaa taactcattg agcaagcgcg gcttattcag caagacgaca
    32101 acttacaagg cgtagggcca tcgctgcctt ttgctttaga tcacaaatac ccttctgctt
    32161 atcgacaagc ggcgtggatg tttgtctttc cctccagcac gctctgcaac cacccgtata
    32221 acggcaaatt atgccgccat catctgcatg actccgttgc gcgaaaggca ttgaaggcag
    32281 ccgtacaaaa agcaggcatc gttagcaagc gtgtcacttg tcatacattt cgtcactcgt
    32341 ttgctacgca tctattacaa gcggggcgtg atattcgcac tgtgcaagaa ctcttagggc
    32401 ataacgatgt taagaccacg caaatctata cgcatgtgtt gggtcagcat tttgccggca
    32461 ccaccagtcc tgcggatgga ctgatgctac ttatcaatca gtaaaagtag ataaatatct
    32521 ttatgtagcc ctgagtagcc cctgatactt tttatggtga aaaagataaa caatcataga
    32581 ggggataaat atgagccagt tctaccattg ttcagataag tttaagcaag atactgtcaa
    32641 tcacagtgac cattcatggt cattacactt cctccagttg ccagacaatg cctaacataa
    32701 aaacgcaaaa gcccaaaagt cagagcttag gtgttctagg cgttttctaa gcgctgaaga
    32761 aatacgtctt accgtagatt gcgtgcgttt ctgaagcaca ttttccttat acatggcttt
    32821 atcccaggca gcttcatcaa gctcttggag taacaagcca gtaataatgc gacactctcg
    32881 ggtttgtagc gagccgctgg tgagatcgga gttatagcgg aaaggggtca tttgtaatta
    32941 tttgtgggtg gaggatagtt aacttattat tgtagttgga agaggctgct ttccaaacat
    33001 tagcacctcg taatgcttgt tactgtatat taatttatgc tcttagcgca tgttaatggg
    33061 ctattacaat gcataatgtc cacatcaaga taactgtggc ttaacacctg tacaagcgaa
    33121 aaaaatcaat cacctgtccg gaaatacggg atcactgtaa agtgttggtg aggcagtcta
    33181 ggagggggat ttaatttggg atactgatga aaggattatg atcagttgga tataggatcg
    33241 tactaagctg agcggcaggt tagctagatt agttaaccag ataagtgaaa tctagttcca
    33301 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
    33361 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
    33421 aactattttg tccgcccaca gctgccg
//
LOCUS       DAJTRW010000041        33147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRW010000041 DAJTRW010000000
VERSION     DAJTRW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(2..94)
                     /locus_tag="OO326_002050"
     tRNA            complement(2..94)
                     /locus_tag="OO326_002050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO326_002051"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO326_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5966436.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO326_002052"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO326_002052"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5966437.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO326_002053"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO326_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5966438.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO326_002054"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO326_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5966439.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO326_002055"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO326_002055"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5966440.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO326_002056"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO326_002056"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5966441.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO326_002057"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO326_002057"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO326_002058"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO326_002058"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5966442.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO326_002059"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO326_002059"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5966443.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO326_002060"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO326_002060"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5966444.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO326_002061"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO326_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5966445.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO326_002062"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO326_002062"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5966446.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO326_002063"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO326_002063"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5966447.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO326_002064"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO326_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5966448.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO326_002065"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO326_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5966449.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO326_002066"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO326_002066"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5966450.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO326_002067"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO326_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5966451.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO326_002068"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO326_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5966452.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO326_002069"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO326_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5966453.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO326_002070"
     CDS             complement(20900..21019)
                     /locus_tag="OO326_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5966454.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO326_002071"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO326_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5966455.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO326_002072"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO326_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5966456.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO326_002073"
     CDS             complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO326_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5966457.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKS
                     VLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIY
                     QSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATC
                     APLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACP
                     LLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKW
                     LNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO326_002074"
     CDS             complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO326_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5966458.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO326_002075"
     CDS             complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO326_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5966459.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26945..27295
                     /gene="hypA"
                     /locus_tag="OO326_002076"
     CDS             26945..27295
                     /gene="hypA"
                     /locus_tag="OO326_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5966460.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27299..28171
                     /gene="hypB"
                     /locus_tag="OO326_002077"
     CDS             27299..28171
                     /gene="hypB"
                     /locus_tag="OO326_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5966461.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28162..28434
                     /gene="hypC"
                     /locus_tag="OO326_002078"
     CDS             28162..28434
                     /gene="hypC"
                     /locus_tag="OO326_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5966462.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28434..29555
                     /gene="hypD"
                     /locus_tag="OO326_002079"
     CDS             28434..29555
                     /gene="hypD"
                     /locus_tag="OO326_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5966463.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29552..30562
                     /gene="hypE"
                     /locus_tag="OO326_002080"
     CDS             29552..30562
                     /gene="hypE"
                     /locus_tag="OO326_002080"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5966464.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30636..32714
                     /gene="flhA"
                     /locus_tag="OO326_002081"
     CDS             30636..32714
                     /gene="flhA"
                     /locus_tag="OO326_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5966465.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32751..33104)
                     /locus_tag="OO326_002082"
     CDS             complement(32751..33104)
                     /locus_tag="OO326_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5966466.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
BASE COUNT         7810 a         9241 c         8626 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagcaggat aatgccgata
    24661 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    24721 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    24781 gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    24841 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    24901 tgcagcggaa taatcccagc cagcaggcca aagccaatca caccgagcag ccagatatcg
    24961 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    25021 cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg cctcagcgca
    25081 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa ggccatgact
    25141 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    25201 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    25261 ttatagaggc tgacaaacag accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc
    25321 ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    25381 gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc gccaattccg
    25441 gctatccagc cacttagcgc tttttgaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25501 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25561 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25621 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25681 acacaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    25741 atacctggca cccagtcaag caatgtgctg acacgcgccg gagcaggcgg tgccggtggc
    25801 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25861 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25921 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    25981 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattgagcat cactctcagg
    26041 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg
    26101 tggcagccga tacagagcgt ggagtcggca attacaaaac gattcaccag gcattcctca
    26161 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    26221 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    26281 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    26341 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    26401 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    26461 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26521 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26581 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26641 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26701 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttactcgttt aacagagatg
    26761 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    26821 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26881 cgcagggata atcaacactg gcgcaattat tgcttgtagc tggcaatagt taatgggagg
    26941 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    27001 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    27061 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    27121 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    27181 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    27241 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    27301 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    27361 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgcaga tgaaaatcac
    27421 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27481 tcatggcgaa gcgggcactc acacaccggg catgagccag cgtcggatgc tggaagtcga
    27541 aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27601 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27661 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggcgacca
    27721 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggt acaccagcga ttcaggtgaa
    27781 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27841 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27901 cttcgatctc ggtgaaaaac acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa
    27961 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    28021 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    28081 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    28141 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    28201 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    28261 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    28321 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    28381 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    28441 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28501 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    28561 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28621 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28681 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    28741 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28801 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa gtggtgttct
    28861 tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa caggcgaaag
    28921 cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc ccgacgctgc
    28981 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    29041 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    29101 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    29161 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    29221 acctactggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    29281 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    29341 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    29401 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    29461 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29521 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    29581 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29641 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    29701 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29761 taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcgggca gtggcgctat
    29821 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29881 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    29941 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    30001 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    30061 gctggttagc ggtacactcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    30121 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    30181 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt
    30241 ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttctgaat cagcgctgcc
    30301 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    30361 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    30421 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    30481 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    30541 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30601 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30661 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30721 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30781 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca
    30841 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    30901 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    30961 ccgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcgggag
    31021 ggcatatttt tggtggctgt gaatttattc gttatgacga gcgtccctgg agcgaaaaag
    31081 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    31141 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    31201 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    31261 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagcatt gtcttacgca
    31321 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    31381 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    31441 aagagatgct gctgatcaat ctctacgagc gggacgattt agccccctat gaacgcatgt
    31501 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    31561 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    31621 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    31681 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    31741 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    31801 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    31861 gtgaaactgg ctcgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    31921 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    31981 atctgtttgg tcatgagcgt ggggctttta ccggtgccaa cgcccagcgc attggtcgtt
    32041 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    32101 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    32161 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    32221 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    32281 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    32341 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    32401 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag
    32461 tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgct ttaccggagc
    32521 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    32581 ttgtgcgcgt gctgaaagaa accaacagcg tggttgccgg gcctaaaggc gctgcgcaac
    32641 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    32701 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    32761 ggttttttcg gacgatattt ttccggcagt tctggcaccg gacacttgtc atcgatgaga
    32821 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    32881 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    32941 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    33001 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    33061 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccacctcat
    33121 taagatgcat ttatattaca tcttaat
//
LOCUS       DAJTRW010000042        31179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRW010000042 DAJTRW010000000
VERSION     DAJTRW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            393..2051
                     /locus_tag="OO326_002083"
     CDS             393..2051
                     /locus_tag="OO326_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5966467.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="OO326_002084"
     CDS             complement(2048..3004)
                     /locus_tag="OO326_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5966468.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="OO326_002085"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="OO326_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5966469.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="OO326_002086"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="OO326_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5966470.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO326_002087"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO326_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5966471.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="OO326_002088"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="OO326_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5966472.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="OO326_002089"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="OO326_002089"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5966473.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9863..9950)
                     /locus_tag="OO326_002090"
     tRNA            complement(9863..9950)
                     /locus_tag="OO326_002090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9914..9916),aa:Ser,seq:gga)
     gene            complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO326_002091"
     CDS             complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO326_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5966474.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO326_002092"
     CDS             complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO326_002092"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5966475.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO326_002093"
     CDS             complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO326_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5966476.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO326_002094"
     CDS             complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO326_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5966477.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO326_002095"
     CDS             complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO326_002095"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5966478.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="OO326_002096"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="OO326_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5966479.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21230
                     /gene="ftsK"
                     /locus_tag="OO326_002097"
     CDS             17202..21230
                     /gene="ftsK"
                     /locus_tag="OO326_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5966480.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21385..21996
                     /gene="lolA"
                     /locus_tag="OO326_002098"
     CDS             21385..21996
                     /gene="lolA"
                     /locus_tag="OO326_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5966481.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22007..23350
                     /gene="rarA"
                     /locus_tag="OO326_002099"
                     /gene_synonym="mgsA"
     CDS             22007..23350
                     /gene="rarA"
                     /locus_tag="OO326_002099"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5966482.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23441..24733
                     /gene="serS"
                     /locus_tag="OO326_002100"
     CDS             23441..24733
                     /gene="serS"
                     /locus_tag="OO326_002100"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5966483.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24973..27416
                     /gene="dmsA"
                     /locus_tag="OO326_002101"
                     /pseudo
     CDS             24973..27416
                     /gene="dmsA"
                     /locus_tag="OO326_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27427..28044
                     /gene="dmsB"
                     /locus_tag="OO326_002102"
     CDS             27427..28044
                     /gene="dmsB"
                     /locus_tag="OO326_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5966484.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28046..28837
                     /locus_tag="OO326_002103"
     CDS             28046..28837
                     /locus_tag="OO326_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5966485.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28873..29499)
                     /locus_tag="OO326_002104"
     CDS             complement(28873..29499)
                     /locus_tag="OO326_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5966486.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29814..30962
                     /locus_tag="OO326_002105"
     CDS             29814..30962
                     /locus_tag="OO326_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966487.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7644 c         8370 g         7476 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9901 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9961 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    10021 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat
    10081 ctacatcgag acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata
    10141 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    10201 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    10261 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    10321 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10381 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10441 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10501 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10561 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10621 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10681 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc
    10741 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10801 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10861 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10921 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10981 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg
    11041 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    11101 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    11161 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    11221 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    11281 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    11341 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11401 accaagcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11461 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct
    11521 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11581 gatgggtgac cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct
    11641 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11701 catgtaacag cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11761 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    11821 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11881 cacgcagtgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11941 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    12001 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    12061 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    12121 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    12181 gagtttgggt atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    12241 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    12301 caaacgcggc taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc
    12361 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    12421 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12481 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12541 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12601 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12661 gtaacataac gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12721 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    12781 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg
    12841 ccagtccggc aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca
    12901 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12961 tgattgcggc accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    13021 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    13081 tggcgagcag cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt
    13141 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    13201 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    13261 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    13321 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13381 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13441 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13501 cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13561 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13621 cccgcaattt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13681 atgattctgg tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13741 agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca
    13801 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13861 ccggcgacgt gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt
    13921 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13981 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    14041 aaaactctgg cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt
    14101 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    14161 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt
    14221 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    14281 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    14341 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg
    14401 ccataaacaa cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14461 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14521 gatagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    14581 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14641 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14701 agaccaccca tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14761 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14821 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14881 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca
    14941 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    15001 aaataaaggc gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta
    15061 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    15121 gccagtaccg gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc
    15181 aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    15241 gtagccgttt tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat
    15301 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15361 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15421 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15481 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15541 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15601 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15661 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15721 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15781 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa
    15841 cggaccggtc agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag
    15901 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15961 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    16021 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    16081 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    16141 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    16201 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    16261 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccacagca gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc
    19621 cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac
    19681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    19741 acaaaccgac gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    19801 agccggtaga tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg
    19861 atttccgtat taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg
    19921 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    19981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    20041 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    20101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    20161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    20221 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    20281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    20341 gcattccgca cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    20401 gctggtgtgt taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta
    20461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    20521 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    20581 catacattgt cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    20641 tggaagagct gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    20701 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    20761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    20821 gcgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc
    20881 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    20941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    21001 gtggtgcggg tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    21061 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    21121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    21181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    21241 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    21301 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    21361 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca
    21421 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    21481 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    21541 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    21601 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    21661 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    21721 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    21781 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    21841 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    21901 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    21961 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    22021 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    22081 agcacagtat atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    22141 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    22201 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    22261 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    22321 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    22381 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    22441 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct
    22501 gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta
    22561 tggtggtcag gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa
    22621 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta
    22681 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    22741 cgcccgcttt gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc
    22801 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    22861 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    22921 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    22981 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    23041 aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga
    23101 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    23161 cgggcaggaa tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    23221 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    23281 caagattggt gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    23341 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    23401 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    23461 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    23521 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    23581 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    23641 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    23701 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    23761 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    23821 taccccgcgt gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc
    23881 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    23941 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    24001 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    24061 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    24121 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    24181 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    24241 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    24301 cgacaaagtt gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga
    24361 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    24421 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    24481 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    24541 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    24601 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    24661 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    24721 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt
    24781 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    24841 gaaaatctac ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga
    24901 gctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt
    24961 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc
    25021 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    25081 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    25141 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    25201 gtcgtggacg gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg
    25261 gcctacacta ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc
    25321 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25381 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25441 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25501 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25561 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25621 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25681 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25741 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25801 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25861 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25921 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25981 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    26041 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    26101 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    26161 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26221 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26281 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26341 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26401 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26461 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26521 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26581 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26641 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26701 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26761 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26821 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26881 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26941 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    27001 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    27061 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    27121 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27181 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27241 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27301 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27361 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27421 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27481 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27541 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27601 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27661 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27721 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27781 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27841 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27901 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27961 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    28021 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    28081 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    28141 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28201 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28261 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28321 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28381 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28441 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28501 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28561 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28621 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28681 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28741 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28801 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28861 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28921 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28981 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    29041 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    29101 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    29161 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29221 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29281 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29341 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29401 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29461 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29521 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29581 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29641 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29701 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29761 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29821 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29881 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29941 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    30001 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    30061 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    30121 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30181 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30241 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30301 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30361 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30421 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30481 tgcctctgta cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg
    30541 tactggtcag tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg
    30601 gtcgactgct ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    30661 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    30721 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga
    30781 accaagcctt actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta
    30841 tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta
    30901 tctatttgct gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt
    30961 aaatgaattc aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta
    31021 tctgacttgc ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa
    31081 ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga
    31141 tgccccttta attttggcga aggatttgtc tatggctgg
//
LOCUS       DAJTRW010000043        30298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRW010000043 DAJTRW010000000
VERSION     DAJTRW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="OO326_002106"
     CDS             complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="OO326_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5966488.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGT"
     gene            complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="OO326_002107"
     CDS             complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="OO326_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5966489.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="OO326_002108"
     CDS             complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="OO326_002108"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5966490.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="OO326_002109"
                     /pseudo
     CDS             complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="OO326_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="OO326_002110"
     CDS             complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="OO326_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5966491.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="OO326_002111"
     CDS             complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="OO326_002111"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5966492.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6044..7183)
                     /gene="wza"
                     /locus_tag="OO326_002112"
     CDS             complement(6044..7183)
                     /gene="wza"
                     /locus_tag="OO326_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5966493.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7492..7641
                     /locus_tag="OO326_002113"
     CDS             7492..7641
                     /locus_tag="OO326_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966494.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7842..9425
                     /locus_tag="OO326_002114"
     CDS             7842..9425
                     /locus_tag="OO326_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5966495.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="OO326_002115"
     CDS             complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="OO326_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5966496.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="OO326_002116"
     CDS             complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="OO326_002116"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5966497.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12425..13066)
                     /gene="udk"
                     /locus_tag="OO326_002117"
     CDS             complement(12425..13066)
                     /gene="udk"
                     /locus_tag="OO326_002117"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5966498.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13384..13626
                     /locus_tag="OO326_002118"
                     /pseudo
     CDS             13384..13626
                     /locus_tag="OO326_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13625..14566
                     /gene="mdtA"
                     /locus_tag="OO326_002119"
                     /pseudo
     CDS             13625..14566
                     /gene="mdtA"
                     /locus_tag="OO326_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14566..17688
                     /gene="mdtB"
                     /locus_tag="OO326_002120"
                     /pseudo
     CDS             14566..17688
                     /gene="mdtB"
                     /locus_tag="OO326_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17689..20754
                     /gene="mdtC"
                     /locus_tag="OO326_002121"
     CDS             17689..20754
                     /gene="mdtC"
                     /locus_tag="OO326_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5966499.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20755..22170
                     /locus_tag="OO326_002122"
     CDS             20755..22170
                     /locus_tag="OO326_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966500.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22167..23570
                     /gene="baeS"
                     /locus_tag="OO326_002123"
     CDS             22167..23570
                     /gene="baeS"
                     /locus_tag="OO326_002123"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5966501.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23567..24289
                     /gene="baeR"
                     /locus_tag="OO326_002124"
     CDS             23567..24289
                     /gene="baeR"
                     /locus_tag="OO326_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5966502.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24480..24812
                     /locus_tag="OO326_002125"
     CDS             24480..24812
                     /locus_tag="OO326_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5966503.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24959..26320
                     /gene="yegQ"
                     /locus_tag="OO326_002126"
     CDS             24959..26320
                     /gene="yegQ"
                     /locus_tag="OO326_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5966504.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26662..26979)
                     /locus_tag="OO326_002127"
     CDS             complement(26662..26979)
                     /locus_tag="OO326_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966505.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27385..28284
                     /gene="yegS"
                     /locus_tag="OO326_002128"
     CDS             27385..28284
                     /gene="yegS"
                     /locus_tag="OO326_002128"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5966506.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28366..29139)
                     /locus_tag="OO326_002129"
     CDS             complement(28366..29139)
                     /locus_tag="OO326_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5966507.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29245..30285)
                     /gene="gatD"
                     /locus_tag="OO326_002130"
     CDS             complement(29245..30285)
                     /gene="gatD"
                     /locus_tag="OO326_002130"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5966508.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7010 a         8049 c         7830 g         7409 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttattcaga
      121 tattgaaatg cccactccag caaataaaac aggataaagg tcataacccc tgaaaacgat
      181 ccggataata ttatccctgc gagaatcagg gcatctgttt taggcgtttt gataccaaac
      241 tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa
      301 agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa
      361 atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat aatttgcgcc
      421 atctccaccg ccgccagcgc caccaccagt ccgaccacca gataaaagaa acgtaatatc
      481 ttacgatggt tgtgtggcga aattgtttta aaacgaatac tccacaccat gccaataatg
      541 atcacaatat agacaaacag catagttgaa gtgacgtatt taccggcatc caacgactga
      601 ccaaacaggt agttaaacgc cgtgagtccc gcgccaatcc ctaaggcaat catcaatttt
      661 ttaatgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt gacgatggtg
      721 atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagtggc
      781 agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc
      841 actgtaggcg gcgcgactgc gttggctgaa ctcagccagc gtggtaccaa atatcgcctg
      901 cgcgatttcc ggtttgctta actgtaccag ttgcagcacc tcttcttcac tgacggtttt
      961 accaccggat ttttgcagca cttcccgcgc cgcatcgcta tgggtggcaa tcaccggcac
     1021 gccaatcgat agcgcctcac acaaaatcag cgggtagtta tcgacacgag aactgaacac
     1081 cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt caaagccgtg
     1141 attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt
     1201 gtcgcccagc gccatcatct cacgcaccag ttgctggtta gttttgccgt cgtaacgcag
     1261 gtcatgcgcc accactgcga ttttcggctt gccctgagtt tcgcgcaccg gaggcagatc
     1321 tgccagaatc gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg
     1381 cccgtacaga ctattgaaag cgtcagccac atgctggctg ggggaaataa actgacagcc
     1441 cagcgccagc atctcgcgga ataactggcg tttgcccgcc accagctggt gcgcgcgatc
     1501 aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc
     1561 ttcgcaaccg tcggtaaagg cgcagcgtcc ggttacactc cagtggtcgt gcagcgtcca
     1621 gaccagggtg acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctgtt
     1681 aagattcaac cagtagctgt gcagcacatg aaaatgcagg accaccggac ccggtgtgcg
     1741 agtaatggtg cgatataact cattgaaatt gccgaacaga tcgcgattaa acagacgaaa
     1801 caacgcaatg ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct gcggatagtt
     1861 ctgatggctg acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag
     1921 cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc cgccttccgc
     1981 cagtcgcaca ttaaattgca gaatattcat ttaatcacct tcactcgcgc tttttctccc
     2041 accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc cacggtgacg
     2101 ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg
     2161 ccaatgtgtg gacatgccat gttatcggca ccccgattgc cgatagtgac gccgtggcga
     2221 atggtgaaat catcccccgc caccacgttt ttattgatca ctacggcgta accgtgatgg
     2281 atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg
     2341 gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag gacgttcttt
     2401 ttgcgccaca ccgaacaaaa atgagcgacg cgataggcaa gtaccatgca gcacgggcgt
     2461 aaactccagc tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag
     2521 ccagacgttt gccgttacgc accgacagca gcgtcaacaa ggtgcgccag gtcatacgtt
     2581 tattgcggat ctggtagagg gtaaacagct gatatttttt actggcgcgg tcgaatttgt
     2641 ctttgtgctt gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct
     2701 gcatttcgcc gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc
     2761 cgtactccac caccatccgc aggaatatgt cgtaatcctg tgctgctttc agttcggtat
     2821 cgaacaaaca ctctttgaaa cgccatgccc aggtaaagac ctgattgcca atgatattac
     2881 gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag ctcgccggtt
     2941 gggaataaac ttctccctag caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca
     3001 gttgctgttt atgggcgagg aagacgctca ggcggttggg cgtccattca tcatcatcat
     3061 cgatcccggt gatgtattcc ccttgcgcca gcataatcgc ctggttacgc accgcgcacg
     3121 ccccgctgtt aatgtcgttg tggatgtaag tgatacgcgg atcgttgagg gcagtgacgt
     3181 actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgc
     3241 tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg
     3301 ttggcatata gattgagatc agcggattgt ctttcatagg ttgttctccc ggcttatgag
     3361 ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc ggtgcgaatg
     3421 caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3481 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3541 ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat aacgcgccac
     3601 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3661 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3721 gcgttcgctc attaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3781 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3841 ctccgacagc ccattgacat tattggtgcc caacagctcg tgggtgtagc ctttgcgcat
     3901 atcgcagtcg atcaacaaca cgcgtttatt ggtctggctg atcaccgccg ccaggttggc
     3961 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     4021 cgcctgcatc atcgcgaagt gcagactggt acgcagacta cggatggctt caatcgccag
     4081 atcggttgga ttccccactg ccagtagctg gctctgttta tagcgtttaa cccctttgat
     4141 ggttttgacg ctatcgcgcg ctttctgcca ctccgacagc gggatgctgg catagacgct
     4201 gattccgtgt tcttccagca cttgcgggct ttcgatgccg cgattaaaca acgagcgcag
     4261 cagcacaccc acgatagaga gcatcaggcc aaggataatc gcgccgagga taatcagccc
     4321 tttcttcggt tttagcacgc caggctgggt gattgccggg tcaataatgc gcacatcgcc
     4381 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     4441 ctgctgacca gactcgacat cgcgggtcag acggacaatt tcctgctggt ttttcggcat
     4501 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4561 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4621 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4681 aagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4741 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4801 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgagcgatgc tgttaagaat
     4861 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4921 gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca acgtggagta
     4981 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     5041 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     5101 cagggtgtag tttttgtcat ccagcacatt aagcgtaaac acctgatccg ccatctcttt
     5161 cgggcggtta aaggtagtca ctttcaccgt ctcgttctga cgccccatca ggcgatccca
     5221 gcccgccccg aaaatcggga acgtgttttt gctcaccgca atatcgaggt cgagatcgtc
     5281 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     5341 cggtttgttg gctaatgccg agccgatatc ctgcaccagc gaattgccgc tgttttgctc
     5401 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaagtgt aaaccacggc
     5461 acagagggca aatacgacgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5521 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5581 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5641 cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgtgata cggatcgggg
     5701 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctccggtgcc
     5761 atctcgcata agcgttcgat atggcgctta tccatggtca aaatcaggtc atagttgcga
     5821 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5881 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5941 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccatcgg ggaacggcaa
     6001 atattgccga cacagacaac taagatgttg ttaaacatga cgattaccag ttatgaatgt
     6061 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcagcaac tgattgatca
     6121 gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa ggttgcaggc
     6181 ggaattctgt cgccatcacc agcgacgtcg catcggacat atccagctgg tagatattgg
     6241 caatcttgcc gttacgcccg ccctcgcctt tcagcggacg aatgacaaag atgccgctgg
     6301 cgttggaggt ggtcatgtcg ataccttcgg cattggccag agcttcagtc agggtcatgc
     6361 cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca aatactttca
     6421 gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg ttctgattga
     6481 ggtcgccgtt ttgcatcagc gcctgtaaag aaatgcgttc ttcacgacca ttgtgtgtta
     6541 gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg atggcgtcga
     6601 gaatggtcag tggtacgttg gtgatcgcct gttgaccgga tttattcacc tgacctgaga
     6661 tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg tcagcgatgt
     6721 acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc ccgactacgt
     6781 gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag ttgccggtgt
     6841 cgctggagct gcggtactga ccagctggcg tggtgagttc cgggtgatcc cagacggtga
     6901 cattaagaac gtcccccggc ccgacgcgat actggtaatt cgcgatctcg ctttccagcg
     6961 tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg cgcggggtca
     7021 gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt ttgatgacgt
     7081 ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt atcaaggtta
     7141 ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat ttatcacttt
     7201 ggcagagtaa ttatcctgtg cactattaat agcaatgtcg ccatgcacat ttaccttgca
     7261 gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc atccgcaggc
     7321 tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc gcccctggct
     7381 ttttagctac caatacactg attgagctta atttttcaaa ccctctctgc atacaatcgt
     7441 tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat tatggtaatt
     7501 gaatattggc tcgccaataa tgcaggagga agtgttacag ctaacggaat agcaggcaag
     7561 gtaacaattc ggcaattggc tatttttaag aattatatta agtatcattc aatatggttt
     7621 ttaggagttt ctttaggttg acaatattta atattgcgtc tccacatgcg atatttctta
     7681 aataatgttt tattattacc gcttttaatt cagggataat gtgaggttat taccctcaaa
     7741 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
     7801 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
     7861 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
     7921 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
     7981 gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctagcg tcaatctcct
     8041 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
     8101 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
     8161 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
     8221 gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca gtgattaccg
     8281 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
     8341 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
     8401 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
     8461 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcac
     8521 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaatcag acgctacgcc
     8581 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg
     8641 acgccaaaac cgcgtccatg ctggtggatc atggaaacca gcagatcttt aatccgcagg
     8701 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtaagc agcattatga
     8761 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt
     8821 tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac gcagaagatt
     8881 tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc actgcgcggc gaaccgctca
     8941 acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttaccag
     9001 ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat gagtttggct
     9061 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     9121 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     9181 cggcgaatgg tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg
     9241 aaaaacgtga atatcacacc attgccgggc tgttgatgga gtatttgcag cgtattccaa
     9301 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     9361 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggcgagatg gagtacgagg
     9421 tgtgatggta tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa
     9481 gcgttgtctg tggtaaacgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga
     9541 atcgtttcct gtaggctggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
     9601 gatgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag
     9661 cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca
     9721 cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg aacagccttc
     9781 gatcttacat attcagaata taatagtgcc gccgcgaacg cctgatgcga cgctttacgc
     9841 gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt
     9901 cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt
     9961 cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt aattgagttg
    10021 ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt
    10081 gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc
    10141 tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac cttgcatgtc
    10201 gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagccgcgt
    10261 cacgttatcg aagttttcag cggccttcac atcaccgcca ttacgctcta ccgcttgctg
    10321 aatcatctgc tggaagttca tcccttccat acgtgtgtcg gtcatctcaa catgcgcctg
    10381 tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc
    10441 cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat
    10501 ctcaacgttc tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag
    10561 cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag taatttccag
    10621 caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg
    10681 cacgttactg gcctgcaaca aaatatcagc tgtaaagcct tgcagtccct gataggccgg
    10741 ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccctgctg
    10801 cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt caagattcag
    10861 ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga cctgtgcttg
    10921 tccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg
    10981 tgactcctgc cactgggact ggaaactccc ctgcccctga ataccttgtt ttggtaaatc
    11041 ggcaccctgc aactgccagt taatttgttc aatagccgct gttaaatcat gcggataatc
    11101 agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaacccg
    11161 cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt
    11221 gcggattgtc acctgctcgt catcttcatg ctggaacacc agaacgctgt ccgccacctt
    11281 aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc
    11341 gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg tcagttggat
    11401 cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta gtggtaaaag
    11461 cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt
    11521 gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac gcagtggccc
    11581 gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa
    11641 atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca cgaccaagag
    11701 tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt
    11761 atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt
    11821 gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc
    11881 cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa tgcaaccaga
    11941 ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac gcgccaaaga
    12001 ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact ccagtgtcac
    12061 cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc
    12121 atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat
    12181 aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta catccaccgt
    12241 cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag
    12301 ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg cacatcactc
    12361 ccttaacgcc aattacgtca agggcatacc aacttatcac tgaacggtac acaatttatc
    12421 aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg atgcggtttt
    12481 tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga
    12541 acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa cgcccacgct
    12601 cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa
    12661 tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa
    12721 tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc
    12781 tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc tcaagcaaca
    12841 ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca
    12901 gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga
    12961 cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg
    13021 cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct
    13081 gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt
    13141 tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa
    13201 gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca
    13261 gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa
    13321 cttttgtact agcataaaca cagaaacgaa tactggcgac ctggccttgc ggataaagcg
    13381 gtaataagca aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg
    13441 gtcagtttag gtttagtctc gtttatcttt acccttgtct cgcttgagct ttcgcagttt
    13501 ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc gttttatcgc
    13561 catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa tatcgccgca
    13621 tccattccag gaaggccagc aggtcaaagc aggcgattta ctggcagaaa ttgaccccag
    13681 ccagttcaaa gttgcattag cacaaaccca gggccaactg gcaaaagata aagccacgct
    13741 tgctaacgcc cgccgtgatc tggcgcgtta tcagcaactg gcaaaaacca atctcgtctc
    13801 ccgtcaggag ctggatgccc aacaggcgct ggtcagtgaa accgaaggca ccattaaggc
    13861 tgatgaagca agcgtcgcca gcgcgcagtt gcaactcgac tggagccgca tcaccgcacc
    13921 agtcgatggt cgcgttggtc tcaagcaagt tgatgttggt aaccaaatct ccagtggtga
    13981 taccaccgga attgtggtga tcacccagac gcatcctatc gatttagtct ttaccctgcc
    14041 ggaaagcgat atcgctactg tagtgcaggc gcaaaaagcc ggaaaaccgc tgatggtaga
    14101 agcctgggat cgcaccaatt cgaagaaatt aagtgaaggc acgctgttaa gcctcgataa
    14161 ccaaatcgat gccacaaccg gtacgattaa agtgaaagca cgctttaata atcaggatga
    14221 tgcgctgttt cccaatcagt ttgttaacgc gcgcatgtta gtcgacaccg aacaaaacgc
    14281 cgtagtgatc ccaacagccg ccctgcaaat gggcaatgaa ggccattttg tctgggtgct
    14341 gaatagcgaa aacaaggtca gcaaacatct ggtgacgccg ggcattcagg acagtcagaa
    14401 agtggtgatc cgtgcaggta tttctgcggg cgatcgcgtg gtgacagacg gcattgatcg
    14461 cctgaccgaa ggggcgaaag tggaagtggt ggaagcccag agcgccacca ctccggaaga
    14521 gaaagccacc agccgcgaat acgcgaaaaa aggagcacgc tcctgatgca ggtgttaccc
    14581 ccgagcagca caggcggccc gtcgcgcctg tttattatgc gtcctgtcgc caccacgctg
    14641 ctgatggtgg cgatcttact cgccgggatt atcggttatc gcgccctgcc cgtttcggcg
    14701 ctgccggaag tggactatcc gaccattcag gtggtcacgc tctacccagg tgccagcccg
    14761 gatgtcatga cctctgccgt taccgcgccg ctcgaacgcc agttcgggca gatgtctggc
    14821 ctgaaacaga tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt gcagttccag
    14881 ctaacattac cgctcaatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc
    14941 aacttgttgc cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat
    15001 ccgccgatca tgacgctcgc cgtcacctca accgccatgc cgatgacgca agtggaagat
    15061 atggtggaaa cccgcgtcgc gcagaaaatc tcgcagattt ccggcgtcgg cctggtgacg
    15121 ctttccggcg gtcagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc
    15181 ctcggcctga ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggca
    15241 aaaggtagcc tcgacggccc ttcccgtgcg gtcacgcttt ccgcgaacga ccagatgcaa
    15301 tccgccgaag agtatcgcca gctaatcatc gcctaccaga acggcgcgcc aattcgtctg
    15361 ggcgatgtcg caactgtaga gcaaggtgca gaaaacagct ggctcggcgc gtgggcgaac
    15421 aaagaacagg ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc
    15481 gccgacagca ttcggcagat gctgccacag ctcactgaga gtctgccgaa atcggtgaag
    15541 gtgacagtgc tttccgatcg caccaccaat atccgcgcat ccgtcgatga tactcagttt
    15601 gaattgatga tggctatcgc gctggtagtc atgattatct acctgttttt gcgcaatatt
    15661 ccggcgacca tcattcccgg tgttgctgta ccgctgtcgt taatcggcac tttcgcggtt
    15721 atggtgtttc tcgatttttc aatcaataac ctgacactga tggctttaac tatcgccacc
    15781 ggattcgtgg tcgatgacgc catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa
    15841 ggcgaaaaac cgttggcggc ggcgctcaag ggcgcaggtg aaatcggctt taccattatc
    15901 tcgctgacct tctcactgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc
    15961 gggcgactgt tccgcgaatt tgctattacc ctggcggtag cgattttgat ctcagcggtg
    16021 gtgtcgctga ccctgacacc gatgatgtgc gcgcggatgc tcagccagga gtcgttgcgt
    16081 aaacagaaca gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat
    16141 ggtcgtggac tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcactcagc
    16201 acgctgctgc ttagcgtgct gctgtgggtg ttcattccga aaggtttctt cccggtacag
    16261 gacaacggca ttattcaggg cactttgcag gcaccgcaat ccagctcctt tgccaatatg
    16321 gcccagcgac aacgccaggt cgcggacgtg attttgcagg atccggcagt gcaaagcctg
    16381 acctcatttg ttggcgttga tggcactaac ccgtcgctga atagtgcacg tttacaaatc
    16441 aacctcaaac cgttggatga acgtgatgat cgggtgcaaa aagtcatcgc ccgtctgcaa
    16501 acggcggtag ataaagtgcc gggcgtcgat ctcttcctgc aaccaacgca ggacctgacc
    16561 atcgatactc aggtcagccg cacccagtac cagtttacct tgcaggccac gtcactggat
    16621 gcgctcagta cctgggtgcc acagttgatg gaaaaactcc agcaactgcc acagctttct
    16681 gatgtctcca gcgactggca ggacaaaggg ctggtggcgt atgtcaatgt tgatcgcgac
    16741 agcgccagcc gtctggggat cagcatggcg gacgtcgata acgccctgta caacgcgttt
    16801 ggtcagcgac tgatttccac tatttatact caggctaacc agtatcgcgt ggtgctggag
    16861 cacaataccg aaaatacccc aggcctcgcg gcgctggata ccattcgcct gaccagcagc
    16921 gacggtggcg tggtgccgtt aagctcaatt gccaaaattg agcagcgttt tgcgccgctt
    16981 tccatcaacc atctggatca gttcccggta acaaccatct cctttaacgt gccggataac
    17041 tattcgctgg gcgatgcggt gcaggcgatt atggacaccg aaaagacgct gaatctgccg
    17101 gtggatatca ccacgcaatt ctagggcagc accctcgcct tccagtcggc gctgggcagc
    17161 actgtctggc tgattgtcgc ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac
    17221 gagagcttta ttcacccgat caccattctc tcgacgctac ccaccgcagg ggtcggcgcg
    17281 ctgctggcat taatgattgc cggtagcgaa ctggatgtga ttgcgattat cggcattatt
    17341 ttgctgatcg gtattgtgaa gaagaacgcc atcatgatga tcgacttcgc gctggctgct
    17401 gagcgcgagc aaggcatgtc gccgcgcgat gcaatctacc aggcttgtct gttgcgtttt
    17461 cgtccgatcc tgatgaccac cctggcggct ctgcttggcg cgctgccgct gatgttgagt
    17521 accggggtcg gcgcggaact gcgtcgtccg ttaggtatcg gcatggtcgg cggtctgatt
    17581 gtcagccagg tgctgacgct gttcaccacg ccggtgattt atctgctgtt cgaccgcctg
    17641 gcattgtgga ccaaaagtcg ctttgctcgc catgaagagg aggcgtaagt gaagtttttt
    17701 gccctcttca tttaccgccc ggtggcgacg attttactgt cggtcgccat taccctgtgc
    17761 ggcatactgg gtttccgtat gctgccggtc gccccgctgc cgcaggtcga ttttccggtg
    17821 attatggtca gcgcctcgct gcccggtgcg tcgccggaga caatggcgtc ttccgttgcc
    17881 acgccgctgg agcgctcact tgggcgcatt gccggggtta gtgaaatgac ctccagcagt
    17941 tcgctcggca gcacgcgcat tattttgcag tttgattttg accgggatat caacggcgca
    18001 gcgcgtgatg tgcaggcggc gatcaacgct gcacaaagtt tgctgcccag cgggatgccc
    18061 agccgcccga cctatcgcaa agcgaacccg tcggatgcgc caattatgat cctcacgctg
    18121 acatccgata cttattcgca gggtgaactg tacgatttcg cctcgacgca gctggctccg
    18181 acgatttcgc aaatcgacgg tgtcggtgat gtcgatgtcg gtggcagctc gctgcccgcc
    18241 gtgcgcgtcg ggctgacccc gcaggcgctg tttaatcagg gcgtgtcgct ggacgacgta
    18301 cgcactgcca tcagcaatgc caacgtgcgt aaaccgcagg gggcgctgga agatggcact
    18361 caccgctggc agatccagac caacgatgaa ctgaaaaccg ccgctgaata tcagccgctg
    18421 attattcact acaacaacgg cggcgcggtt cgtctgggcg atgtggcgac ggtgactgac
    18481 tcagtgcagg atgtgcgcaa cgccgggatg accaacgcca aaccggctat tttactgatg
    18541 atccgcaaac tgccggaagc caatattatc cagacggtag acagcatccg ggcaaaatta
    18601 ccagagttgc aggagaccat tccggcggcg attgatctgc aaattgccca ggatcgctcc
    18661 cccaccattc gcgcctcgct ggaagaagtc gagcaaacgc tgattatctc ggtggcgctg
    18721 gtgattctgg tggtcttttt attcctgcgc tcgggtcgcg ccactattat tcccgccgtt
    18781 gcggtgccgg tttcgctgat tggtacgttt gcggcgatgt acctgtgcgg attcagtctc
    18841 aataatcttt cgctgatggc gctcaccatt gctactggtt tcgtggtgga tgacgccatt
    18901 gtggtgctgg aaaacattgc ccgtcatctg gaagcgggga tgaaaccact acaggccgcg
    18961 ctgcaaggta ctcgcgaagt cggctttacg gtgctgtcga tgagtctgtc actggtggcg
    19021 gtgttcctgc cgctgctgtt gatgggcgga ttgccgggcc gactgttacg cgagtttgcc
    19081 gtgacgcttt ctgtcgccat tggtatttcg ttgctggttt ccctgacgct aacgccaatg
    19141 atgtgcggct ggatgctaaa agccagcaag ccgcgtgagc aaaagcgact gcgtggtttt
    19201 ggccgcatgt tggtagccct gcaacaaggc tacggcaagt cgctgaaatg ggtgctcaat
    19261 catacccgtc tggtgggcgt ggtgctgctt ggcaccattg cgctgaatat ctcgatcccg
    19321 aaaaccttct tcccggagca ggacaccggc gtactgatgg gtgggataca ggcggatcag
    19381 agtatttcgt ttcaggcgat gcgcggcaaa ttgcaggatt tcatgaaaat tatccgtgac
    19441 gatccggcag tggataatgt caccggcttt accggcggtt cacgagttaa cagtgggatg
    19501 atgtttatca ccctcaagcc acgcgacgaa cgcagcgaaa cggcgcagca aattatcgac
    19561 cgtctgcgcg tgaagctggc gaaagaaccg ggggcgaatc tgttcctgat ggcggtacaa
    19621 gatattcgcg ttggtgggcg tcagtcgaac gccagctacc agtacacgtt gttatccgac
    19681 gacctggcgg cactgcgcga atgggagccg aaaatccgca aaaaactggc gacgttgccg
    19741 gaactggcgg acgtgaactc cgatcagcag gataacggcg cggagatgaa tctggtttac
    19801 gaccgcgaca ccatggcacg gctgggaatc gacgtacagg ccgccaacag tctgttaaat
    19861 aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca gtataaagtg
    19921 gtgatggaag tggatccgcg ctatacccag gacatcagtg cgctggaaaa aatgttcgtt
    19981 atcaataacg aaggcaaagc gatcccgctg tcgtatttcg ctaaatggca accggcgaat
    20041 gccccactat cggtgaatca tcagggatta tcggcggcct cgaccatctc gttcaacctg
    20101 ccgaccggaa aatcgctctc ggacgccagt gcggcgatcg atcgcgcaat gacccagctt
    20161 ggtgtgcctt cgacggtgcg cggcagtttt gccggcacgg cgcaggtttt ccaggagacg
    20221 atgaactcgc aggtgatcct gattatcgcc gccatcgcca cggtgtatat cgtgctgggt
    20281 atcctttacg agagttacgt acatccgctg acgattctct ccaccctgcc ctcggcgggc
    20341 gttggagcgc tgttggcgct ggagctgttc aatgccccgt tcagcctaat cgccctgata
    20401 gggatcatgc tattaatcgg catcgtgaag aaaaacgcca ttatgatggt cgattttgcg
    20461 cttgaagccc aacggcacgg taacctgacg ccgcaggaag ctattttcca ggcctgtctg
    20521 ctgcgttttc gcccgattat gatgaccacc ctggcggcgc tgtttggcgc gctgccgctg
    20581 gtattgtcgg gcggtgacgg ctcggagctg cggcaacccc tggagatcac cattgtcggc
    20641 ggtctggtga tgagccagct cctgacgctg tataccacgc cggtggtgta tctctttttc
    20701 gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga gtaaatgaca
    20761 gatcttcccg acagcacccg ctggcaatta tggattgtgg ctttcggctt ctttatgcag
    20821 tcgctggaca ccaccatcgt aaacaccgcc cttccctcaa tggcgcaaag cctcggggaa
    20881 agtccgttgc atatgcacat ggtcattgtc tcttatgtgc tgaccgtggc ggtgatgctg
    20941 cccgccagcg gctggctggc ggacaaagtc ggcgtgcgca atattttctt taccgccatc
    21001 gtgctgttta ctctcggttc actgttttgc gcgctttccg gcacgctgaa cgaactgttg
    21061 ctggcacgcg cgttacaggg cgttggcggt gcgatgatgg tgccggtcgg caggttgacg
    21121 gtgatgaaaa tcgtaccgcg cgagcaatat atggcggcga tgacctttgt cacgttaccc
    21181 ggtcaggtcg gtccgctgct cggtccggcg ctcggcggtc tgctggtaga atacgcgtcg
    21241 tggcactgga tctttttgat caacattccg gtggggatta tcggtgcgat cgccacattg
    21301 atgttaatgc cgaactacac catgcagacg cggcgctttg atctttccgg ctttttattg
    21361 ctggcggttg gcatggcggt attaaccctg gcgctggacg gcagtaaagg tacaggttta
    21421 tcgccgctgg cgattgctgg cctggtcgca gttggcgtgg tggcactggt gctttatctg
    21481 ctgcacgcca gaaataacaa ccgcgccctg ttcagtctga aactgttccg cactcgtacc
    21541 ttttcgctgg gcctggcggg gagctttgcc ggacgtattg gcagtggcat gttgcccttt
    21601 atgacaccgg ttttcctgca aattggcctc ggtttctcgc cgtttcatgc cggactgatg
    21661 atgatcccga tggtgcttgg cagcatggga atgaagcgaa ttgtggtaca ggtggtgaat
    21721 cgctttggtt atcgtcgggt actggtagcg accacgctgg gtctgtcgct ggtcaccctg
    21781 ttgtttatga ctaccgctct gttgggctgg tactacgttt tgccgttcgt cctgttttta
    21841 caagggatgg tcaactcgac ccgtttctcc tccatgaaca ccctgacgct gaaagatctc
    21901 ccggacaatc tggcgagcag cggaaacagc ctgctgtcga tgattatgca attgtcgatg
    21961 agtatcggcg tcaccatcgc cgggctgttg ctggggcttt ttggttcgca gcatatcagc
    22021 gtcgacagcg gcaccacaca aaccgtcttt atgtacacct ggcttagcat ggcgtttatc
    22081 atcgcccttc cggcgttcat ctttgccaga gtgccgaacg atacgcatca aaatgtagct
    22141 atttcgcggc gaaaaaggag cgcgcaatga agttctggcg acccggtatt accggcaaac
    22201 tgtttctggc gattttcgcc acctgcattg tcttgctgat cagtatgcac tgggcggtgc
    22261 gtatcagttt tgagcgtggc tttattgatt acatcaaaca cggcaatgaa cagcgattac
    22321 agctgttaag tgatgcgctt ggcgagcagt atgcgcagca tggtaactgg cgcttcctgc
    22381 gcaacaacga tcgctttgtc tttcagatcc tgcgttcatt tgaacacgat aattcggaag
    22441 ataaacccgg cccgggtatg ccgccgcacg gctggcgtac ccagttctgg gtggtcgatc
    22501 aaaacaacaa agtactggtt ggtccgcgag cgccgattcc acctgacggt acacggcgac
    22561 ccattctggt caacggtgcg gaagtgggcg cggtgattgc ctcccctgtt gagcggctga
    22621 cccgcaatac tgatatcaat ttcgataaac aacagcggca aaccagctgg ctgatcgtcg
    22681 ccctggcaac gttactcgcg gcgctcgcca cctttctgct ggcgcgcggt ttgctggcac
    22741 cggtaaaacg acttgtcgat ggcacgcaca aactggcggc gggcgatttc actacccgcg
    22801 taacgcccac cagtgaagat gaactgggca aactggcgca agacttcaac cagctcgcca
    22861 gcacgctgga gaaaaaccag caaatgcggc gcgattttat ggccgatatt tctcacgaac
    22921 tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc cattcaggat ggcgtgcgca
    22981 aattcacgcc ggagacggtg gcgtctttac aggcggaggt cggtacactg accaaactgg
    23041 ttgacgatct tcatcagttg tcgatgtctg atgaaggcgc tctcgcctat caaaaagcac
    23101 cggtagattt gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa cgattcgcca
    23161 gccgtggcct gaaactgcaa ttttccctgc cagacagtat caccgtattt ggcgatcgcg
    23221 accgtttaat gcagttattc aataacttac tggaaaacag cctgcgctac actgacagcg
    23281 gcggcagcct gaaaatctct gccgagcagc acgacaaaac ggtgcgcctg acctttgccg
    23341 acagtgcgcc aggtgtcagt gacgatcagc tacaaaaatt gtttgaacgt ttttatcgca
    23401 ccgaaggttc ccgcaaccgc gccagcggcg gttccgggct ggggctggcg atttgcctga
    23461 acattgttga agcccataat ggccgcatta ttgccgccca ttcgcctttt ggcggggtaa
    23521 gcattacagt agagttaccg ctggaacggg atttacagag agaagtatga ccgagttacc
    23581 aatcgacgaa aacacaccgc gtattttgat cgtggaagat gaaccgaagc tggggcagtt
    23641 gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc acggcgatca
    23701 ggtactggcg tatgtgcgcc agacaccacc ggatctgatc ctgttggatc tgatgctccc
    23761 tggcaccgat ggcctgacat tatgccggga aattcgtcgt ttttccgatg ttccgatcgt
    23821 gatggtgacg gcaaaaatcg aagagatcga ccgcctgctg gggctggaga ttggcgccga
    23881 tgactacatc tgcaagccgt acagcccacg ggaagtggta gcgcgtgtca aaaccatttt
    23941 gcgccgttgt aaaccgcaac gcgaacttca gcaacaggat gccgaaagcc cgttgattat
    24001 cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga cgcctgcgga
    24061 atttcgtctg cttaaaacgc tctctcacga accaggaaaa gtgttctccc gcgagcaatt
    24121 gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccattg acagccacat
    24181 taaaaatctg cgccgcaagc tggaatctct cgacgccgaa cagtcattta tccgcgccgt
    24241 ttacggcgtt ggttaccgct gggaagccga cgcctgccgc atcgtttagt tttagcgaca
    24301 ttactttgtt agccggagat ggcgttcaga cccgatccgg caacattatt ccacgcatgg
    24361 tcagcaaact gacattatct ctccgccctg gcaggcatca gaaaatcgct catactttaa
    24421 tcagtaaaca gcacctttag atgctgtttt cgataagcaa tttcaatcaa ggagtcatta
    24481 tggctggttg gtttgaactc agtaagagca gtgataatca gttccggttt gtgctaaaag
    24541 cgggcaatgg tgagactatc ctcaccagcg agctgtatac ctcaaaagcc tctgcggaaa
    24601 agggcatcgc gtcggtgcgt agcaacagcc cgcaagaaga gcgctatgag aaaaaaacgg
    24661 caagcaacgg caaattctat ttcaatctga aagccgctaa tcatcaaatt atcggctcca
    24721 gccagatgta cgccaccgcg caatctcgtg aaaccggaat tgcctccgtt aaagccaatg
    24781 gcacaagcca gacggtgaaa gacaatacgt aattacgatg ccgggcgtgg gttgttggcg
    24841 tccggcgctt taccagatcc cccctacagc gctacaatgc ccgcccttaa agtgggggcc
    24901 tcccctaacc gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat
    24961 gtttaaaccg gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc
    25021 ttatggcgca gatgctgttt atgcgggcca gccgcgttac tccctacgtg tgcgcaacaa
    25081 cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa
    25141 gttttatgtc gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga
    25201 cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat
    25261 tatgctggtg cgtgagcact tccctgaaat gccgatccac ctctcggtgc aggctaacgc
    25321 cgtgaactgg gcgacggtga aattctggca gcaaatggga ctgacccgcg tgatcctctc
    25381 tcgcgagctg tcgctggaag agattgaaga gatccataat caggtgccgg atatggagat
    25441 cgagatcttc gttcacggcg cactgtgcat ggcctactcc ggtcgctgcc tgctctctgg
    25501 ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta
    25561 caacgtccag gaagggaaag aagatgacgt tggcaacatc gtacacaagt acgagccgat
    25621 tccggtgcaa aatgttgagc cgacgctggg tatcggcgcg ccaaccgaca aagtgtttat
    25681 gatcgaagaa gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac
    25741 ttacatcatg aactcgaaag atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat
    25801 gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct ttctactatt gcgcacgcac
    25861 cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag
    25921 cctgctggaa actctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg
    25981 tcatactcac gacgattatc agaactacga atacggttat tcagtttctg accgccagca
    26041 gtttgttggt gagtttaccg gtgagcgcaa gggggacctc gcagcggtag cggtgaaaaa
    26101 taaattctcc gttggcgaca gtcttgagct gatgacgccg caaggcaaca ttaactttac
    26161 ccttgagcac atggaaaacg ccaaaggcga agctatgccg gtcgcaccag gcgatggtta
    26221 tactgtgtgg ctcccggtcc cgcaggatct tgagctaaat tacgcgctgc taatgcgtaa
    26281 tttctccggg gaaaccacgc gtaaccccca cggtaagtga ttaatttcga ttatttttcc
    26341 cggatggaaa attcttagaa accgatcaca tacagctgca tttattaagg ttatcatccg
    26401 tttcgctgaa aaacataatc cataaaatgc tagctgtacc aggaaccacc tccttagcct
    26461 gtgtaatctc ccttacacgg gcttattttt tgtacataag ctgtaaagaa ggattgatcc
    26521 ctgataaccg tcttccattg accacatcga tagaatcccc cttcatagca caatgccttt
    26581 cacgtaacgg tatcgtgctc gcccaggttc cggctacgca cagccagaac gcgcatgttt
    26641 gacgcttaca aaaaaatatt ctcactctcc acatttgaat gtcagacgag cgacgccatg
    26701 taatcctgca ccttctgtct tcaggtcaac tatctgcatt tttttgccct gagtaacaca
    26761 gaaatgggct gcatcatttt ttactatatt ttctgcacca gatattctgc ccctggctaa
    26821 agaagcttcg gcttcggtgt agtattggtt atcgagttta cgctgaatat tacttttata
    26881 tgcaagacca aatttaccga tacttgtttc atcattatgc acagcacaac cagatataag
    26941 aaaaatacta attaatgata tagcagctat ctttttcatc tcaccttccc ccattaaata
    27001 ccaacgacac tctctagtgt ttaaatataa taatggcatg attattataa ttgaatagga
    27061 ttataataaa tgttctgtac aacatttcct acataagtag gaattacgga cattgaggcc
    27121 cttcagggta actccatggg ggctttaata tattatattg aagatgccac tgtttagttg
    27181 aatattaggt atatgctctt ttttgaaatt tatcggtggc agccgttagt attcgctgtc
    27241 cccattgcaa gctcctggtg gtaaccactg aatcctccat acttgaactg actttttatc
    27301 ctccgacttt catcctgttc tgactccacc ttttgttttc tgctctacac tatctacaga
    27361 ccaatcataa aggcacatac gatcatggca gaatttcccg ccagcttact gattcttaat
    27421 ggtaaaagta ctgacaatct acccttgcgc gaagcaatta tgctgttgcg tgaggaagga
    27481 atgacgatcc atgtgcgggt cacctgggag aaaggtgatg ccgcacgatt tgtagaggag
    27541 gcccggaagt tgggcgtcgc aacggtgatt gccggtggtg gcgatggcac cattaatgaa
    27601 gtttctacgg cgttgattca gtgtgagggg gatgacatac ccgcgctggg aattttgcca
    27661 ttaggaaccg ccaatgattt tgccaccagt gtagggattc ctgaggcact ggataaggcg
    27721 ctgaaactgg caattgccgg taatgccatt gcgatagata tggcgcaggt caacaaacaa
    27781 acctgtttta ttaatatggc gacaggcgga tttgggacgc gtattaccac agaaacgccg
    27841 gaaaaattaa aagccgcgct gggtggcgtc tcttacatca ttcatggctt aatgcgcatg
    27901 gatactctgc aaccggaccg ttgtgaaatc cgcggtgaaa actttcactg gcaaggtgac
    27961 gccctggtca ttggtattgg taacgggcgt caggccggtg gcggtcaaca attgtgcccg
    28021 aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt ttaccggcga tgaaattctt
    28081 ccggctctcg tatcaacctt aaaatctgac gaagataacc cgaatattat cgaaggcgct
    28141 tcgtcgtggt ttgatataca agccccacac gaaatcactt ttaatcttga tggcgaaccg
    28201 ttgagtgggc aaaacttcca tattgaaata cttccggcgg cgttgcgttg tcgattacca
    28261 ccagattgtc cattattgcg ttaatcaaac tattctctct tgaaccaggg agaatccgta
    28321 atgccggatg cggtgtaaac accttatccg gcatacagaa caatactaca ccgtaattaa
    28381 ttttacccgt gtcttctgca atgccaattt atccacctct gacacagcat catcggtaac
    28441 aataatgtca attttttcga ttggcagtac ctgattaaaa ccgcgacgat taaatttcga
    28501 tgaatcgaga acggcaatca ctttatttgc ggcggtgacc atcgccccgc taatcgaata
    28561 accttcatta aaggtcgtaa taccattaac cgcatcaata ccatcagcac cgacaaacat
    28621 taaatcggca ttaatatcct gcaatgaacg ctcggcaatg gaaccatgca tcgagcgcgt
    28681 tttatggcgg accgtgccac cacagacgac cagagtaatg tctttatttt cggaaagggc
    28741 aaacgctgcc gggagactgt tggtaatcac cgtgatattt ttagcggtca ttaatccttc
    28801 ggcgataagc attgtggtac ttccgctgtc gagaataacg gtcatccctt catggatcat
    28861 tttgactgcc gcctgcgcta ttctgttttt tggcgcgctg gcgagctgaa atcgctcttt
    28921 aaacccgact tcctgggtct cggtttcact attaccggac attattttcg ccgcaccgcc
    28981 atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc aaatcggcac ggattgtcgc
    29041 ttccgaggca gcaaatactc ccgccagatc ctgaacaagc acggttccct gttcattcac
    29101 taattggatg atcttattcc ttcgctcgaa tgaattcatc gtcatttacc catattcaat
    29161 tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat ctgttttgca caataccggg
    29221 ccagcatcac gctggcccgc ggtttcatgg aatgagcaac actttgccct gcatggcatt
    29281 acgagcgatg tcacgcaccg cctgggtgaa gctttcaaag cttccacggt gagcgattaa
    29341 tggctccagg cttaacttac gttctgtcag caaccggctc gccgtttccc actcctgccc
    29401 cggccaaggg ctggaatagt tcatccaact gccgataacc gtcagctctt tacgcaatat
    29461 tttgccaaac gttgccgatg ttaaatgcag atcctgatgc aacgtgccca ccagcgccag
    29521 ttgggcatga ggaccggcaa tctctaccgc cagttcgaca gtttgcggta cgccagccgt
    29581 ctcgaggata agctgattaa agcgcagttc gcgtaaaacg ctctgcattt gcggcgcgct
    29641 catttcgcta ctgttaaatg tttgcatcgc accgaaagat tttgccagtg ccagtttttc
    29701 tgagctaatg tcgatcgccg tcacactctt tgctcccagc gcgacagcgc actgaatagc
    29761 cagcaggcca atggttccgg caccaataat aataacgttt ttattctcac aaccttgcgc
    29821 taaatgaaaa gcatgcaggc caacggtaat cggctcaata aaagccccat cctcaatagg
    29881 catatccgtg ggtagagcaa agacattttt tcgcttaacg acaatatatt cagcaaatcc
    29941 accatcacgc cgcgagccaa taaaatcata ttttgcgcac tgggaataaa accctttcaa
    30001 acactctgga caagtaaaac agggtaataa cggcacacag gcaaccgcat cgccagggtg
    30061 taaatcatca acaccggatc ccaccgcatc aatatagccg ctaaattcat ggcctaacgt
    30121 tattggataa taatgtgcac catttttaaa tatcctgggt aaatcggaac cacataagcc
    30181 cgagctggca atttttaccc gcacctcatc ctgatgttta atttcaggaa tgacgctttt
    30241 tgcaacgcgc acgataccat cagtatcatt cactactgat ttcataaaaa ctcctgat
//
LOCUS       DAJTRW010000044        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRW010000044 DAJTRW010000000
VERSION     DAJTRW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO326_002131"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO326_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5966509.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO326_002132"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO326_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5966510.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO326_002133"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO326_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5966511.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO326_002134"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO326_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5966512.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO326_002135"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO326_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5966513.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO326_002136"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO326_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5966514.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO326_002137"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO326_002137"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5966515.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO326_002138"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO326_002138"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5966516.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO326_002139"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO326_002139"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5966517.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO326_002140"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO326_002140"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5966518.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO326_002141"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO326_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5966519.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO326_002142"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO326_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5966520.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO326_002143"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO326_002143"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5966521.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO326_002144"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO326_002144"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5966522.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO326_002145"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO326_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5966523.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO326_002146"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO326_002146"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5966524.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO326_002147"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO326_002147"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5966525.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO326_002148"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO326_002148"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5966526.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO326_002149"
     CDS             21622..22212
                     /locus_tag="OO326_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5966527.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO326_002150"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO326_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5966528.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO326_002151"
     CDS             22684..23379
                     /locus_tag="OO326_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5966529.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO326_002152"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO326_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5966530.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO326_002153"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO326_002153"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5966531.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO326_002154"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO326_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5966532.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO326_002155"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO326_002155"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5966533.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO326_002156"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO326_002156"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5966534.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTRW010000045        29411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRW010000045 DAJTRW010000000
VERSION     DAJTRW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..213)
                     /locus_tag="OO326_002157"
     CDS             complement(<1..213)
                     /locus_tag="OO326_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5966535.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO326_002158"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO326_002158"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5966536.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO326_002159"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO326_002159"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5966537.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO326_002160"
     CDS             2894..3634
                     /locus_tag="OO326_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5966538.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO326_002161"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO326_002161"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5966539.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO326_002162"
     CDS             complement(4192..4794)
                     /locus_tag="OO326_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5966540.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OO326_002163"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OO326_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5966541.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OO326_002164"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OO326_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5966542.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OO326_002165"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OO326_002165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5966543.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO326_002166"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO326_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5966544.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO326_002167"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO326_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5966545.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OO326_002168"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OO326_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5966546.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OO326_002169"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OO326_002169"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5966547.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OO326_002170"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OO326_002170"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5966548.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO326_002171"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO326_002171"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5966549.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO326_002172"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO326_002172"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5966550.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OO326_002173"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OO326_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5966551.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OO326_002174"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OO326_002174"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5966552.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OO326_002175"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OO326_002175"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5966553.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO326_002176"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO326_002176"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5966554.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OO326_002177"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OO326_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OO326_002178"
     CDS             20653..21009
                     /locus_tag="OO326_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5966555.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OO326_002179"
     CDS             21336..21995
                     /locus_tag="OO326_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5966556.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OO326_002180"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OO326_002180"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5966557.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO326_002181"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO326_002181"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5966558.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OO326_002182"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OO326_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO326_002183"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO326_002183"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5966559.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OO326_002184"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OO326_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5966560.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OO326_002185"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OO326_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OO326_002186"
     CDS             27338..27679
                     /locus_tag="OO326_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5966561.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="OO326_002187"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="OO326_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29115..29396)
                     /locus_tag="OO326_002188"
     CDS             complement(29115..29396)
                     /locus_tag="OO326_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5966562.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7420 a         7228 c         7554 g         7209 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca c
//
LOCUS       DAJTRW010000046        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRW010000046 DAJTRW010000000
VERSION     DAJTRW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO326_002189"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO326_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5966563.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO326_002190"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO326_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5966564.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO326_002191"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO326_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5966565.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO326_002192"
     CDS             4843..5082
                     /locus_tag="OO326_002192"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966566.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO326_002193"
     CDS             5092..5262
                     /locus_tag="OO326_002193"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5966567.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO326_002194"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO326_002194"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5966568.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO326_002195"
     CDS             6724..6990
                     /locus_tag="OO326_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5966569.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO326_002196"
     CDS             7084..7773
                     /locus_tag="OO326_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5966570.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO326_002197"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO326_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5966571.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO326_002198"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO326_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5966572.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO326_002199"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO326_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5966573.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO326_002200"
     CDS             complement(10475..12193)
                     /locus_tag="OO326_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5966574.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO326_002201"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO326_002201"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5966575.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO326_002202"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO326_002202"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5966576.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO326_002203"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO326_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5966577.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO326_002204"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO326_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5966578.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO326_002205"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO326_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO326_002206"
     CDS             18432..18746
                     /locus_tag="OO326_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5966579.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO326_002207"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO326_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5966580.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO326_002208"
     CDS             20570..21862
                     /locus_tag="OO326_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5966581.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO326_002209"
     CDS             complement(22016..23665)
                     /locus_tag="OO326_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5966582.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO326_002210"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO326_002210"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5966583.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO326_002211"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO326_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5966584.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO326_002212"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO326_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5966585.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO326_002213"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO326_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO326_002214"
     CDS             28753..29034
                     /locus_tag="OO326_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5966586.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7285 a         7776 c         7351 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaagtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTRW010000047        28984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRW010000047 DAJTRW010000000
VERSION     DAJTRW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..249
                     /locus_tag="OO326_002215"
     CDS             <1..249
                     /locus_tag="OO326_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5966587.1"
                     /translation="YNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHR
                     VHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            271..1680
                     /locus_tag="OO326_002216"
     CDS             271..1680
                     /locus_tag="OO326_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966588.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1658..2437
                     /locus_tag="OO326_002217"
     CDS             1658..2437
                     /locus_tag="OO326_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5966589.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2434..3294
                     /locus_tag="OO326_002218"
     CDS             2434..3294
                     /locus_tag="OO326_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5966590.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3442..4017)
                     /locus_tag="OO326_002219"
                     /pseudo
     CDS             complement(3442..4017)
                     /locus_tag="OO326_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4034..4294)
                     /locus_tag="OO326_002220"
     CDS             complement(4034..4294)
                     /locus_tag="OO326_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5966591.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4285..5556)
                     /locus_tag="OO326_002221"
     CDS             complement(4285..5556)
                     /locus_tag="OO326_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5966592.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5634..5996)
                     /gene="queD"
                     /locus_tag="OO326_002222"
     CDS             complement(5634..5996)
                     /gene="queD"
                     /locus_tag="OO326_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5966593.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6315..8114
                     /gene="cysJ"
                     /locus_tag="OO326_002223"
     CDS             6315..8114
                     /gene="cysJ"
                     /locus_tag="OO326_002223"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5966594.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8114..9826
                     /gene="cysI"
                     /locus_tag="OO326_002224"
     CDS             8114..9826
                     /gene="cysI"
                     /locus_tag="OO326_002224"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5966595.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9900..10634
                     /gene="cysH"
                     /locus_tag="OO326_002225"
     CDS             9900..10634
                     /gene="cysH"
                     /locus_tag="OO326_002225"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5966596.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10898..11050
                     /locus_tag="OO326_002226"
     CDS             10898..11050
                     /locus_tag="OO326_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5966597.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11244..12335
                     /locus_tag="OO326_002227"
                     /pseudo
     CDS             11244..12335
                     /locus_tag="OO326_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12481..13518)
                     /gene="iap"
                     /locus_tag="OO326_002228"
     CDS             complement(12481..13518)
                     /gene="iap"
                     /locus_tag="OO326_002228"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5966598.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13770..14678
                     /gene="cysD"
                     /locus_tag="OO326_002229"
     CDS             13770..14678
                     /gene="cysD"
                     /locus_tag="OO326_002229"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5966599.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14680..16107
                     /gene="cysN"
                     /locus_tag="OO326_002230"
     CDS             14680..16107
                     /gene="cysN"
                     /locus_tag="OO326_002230"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5966600.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16107..16712
                     /gene="cysC"
                     /locus_tag="OO326_002231"
     CDS             16107..16712
                     /gene="cysC"
                     /locus_tag="OO326_002231"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5966601.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16762..17085
                     /locus_tag="OO326_002232"
     CDS             16762..17085
                     /locus_tag="OO326_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5966602.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17279..17590
                     /gene="ftsB"
                     /locus_tag="OO326_002233"
     CDS             17279..17590
                     /gene="ftsB"
                     /locus_tag="OO326_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5966603.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17609..18319
                     /gene="ispD"
                     /locus_tag="OO326_002234"
     CDS             17609..18319
                     /gene="ispD"
                     /locus_tag="OO326_002234"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5966604.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18319..18798
                     /gene="ispF"
                     /locus_tag="OO326_002235"
     CDS             18319..18798
                     /gene="ispF"
                     /locus_tag="OO326_002235"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5966605.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18795..19844
                     /gene="truD"
                     /locus_tag="OO326_002236"
     CDS             18795..19844
                     /gene="truD"
                     /locus_tag="OO326_002236"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5966606.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19825..20586
                     /gene="surE"
                     /locus_tag="OO326_002237"
     CDS             19825..20586
                     /gene="surE"
                     /locus_tag="OO326_002237"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5966607.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20580..21206
                     /gene="pcm"
                     /locus_tag="OO326_002238"
     CDS             20580..21206
                     /gene="pcm"
                     /locus_tag="OO326_002238"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5966608.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21346..22485
                     /gene="nlpD"
                     /locus_tag="OO326_002239"
     CDS             21346..22485
                     /gene="nlpD"
                     /locus_tag="OO326_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5966609.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22548..23540
                     /gene="rpoS"
                     /locus_tag="OO326_002240"
     CDS             22548..23540
                     /gene="rpoS"
                     /locus_tag="OO326_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5966610.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23660..24067)
                     /locus_tag="OO326_002241"
     CDS             complement(23660..24067)
                     /locus_tag="OO326_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5966611.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24214..24807
                     /locus_tag="OO326_002242"
     CDS             24214..24807
                     /locus_tag="OO326_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5966612.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24807..25754
                     /locus_tag="OO326_002243"
                     /pseudo
     CDS             24807..25754
                     /locus_tag="OO326_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26027..26590)
                     /locus_tag="OO326_002244"
     CDS             complement(26027..26590)
                     /locus_tag="OO326_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966613.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26755..27006
                     /locus_tag="OO326_002245"
     CDS             26755..27006
                     /locus_tag="OO326_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5966614.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27008..>28984
                     /locus_tag="OO326_002246"
     CDS             27008..>28984
                     /locus_tag="OO326_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5966615.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGN"
BASE COUNT         7059 a         7188 c         7858 g         6879 t
ORIGIN      
        1 tacaacgttg ttgactgtaa gccggaagag gaaatcgaca agtaccacaa tccgctcaac
       61 aaaatcatct gcgaagaaac cattcgcctc ggcggttcga tggtgcacca ccatggcatt
      121 ggtaaacatc gcgttcactg gagcaagctg gaacacggca gcgcgtgggc gttgctggaa
      181 gggctgaaaa agcagttcga tcctaacggc attatgaata cgggtactat ctatccgatt
      241 gaaaaataat gtatcaggca gcgttccgcg atgacggggc gttgcctttt cggcttctca
      301 ggcgagaagc cgttcttatt accggacaat gaaggggtaa agatgaacac ttcaccggtg
      361 cgaatggatg atttaccgct taaccgtttt cactgtcgaa ttgctacgct cactttcggc
      421 gcacacctga ccgacggtta tgttctaggc gtcattggtt acgccattat tcaacttacg
      481 cccgccatgc aactgacgcc gtttatggcg ggaatgatcg gcggctcggc gctccttggt
      541 ttgtttcttg gcagcctggt tcttgggtgg atctccgacc atattggtcg gcaaaaaatc
      601 ttcaccttca gctttttgct gattacgctc gcttcgttct tgcaattttt tgccaccacg
      661 ccagagcatc ttattgggct gcgcattttg atcggcattg gtctgggagg cgattactca
      721 gtaggtcaca ccttgctggc tgaattttcc ccgcgccgcc atcgcggtat tttgctgggc
      781 gcattcagcg tggtgtggac cgtaggctat gtgttggcaa gtattgccgg acatcacttt
      841 atttccgaaa acccggaggc ctggcgctgg ttgctggcat cggcagctct gcccgcgttg
      901 ttgattacgt tattacgctg gggaacgcca gaatcgccac gttggctact gtgccagggg
      961 cgttttgcag aagctcacgc tatcgtgcat cgctattttg gtccccatgt tttactgggc
     1021 gatgaagtgg taacggcgac ccataaacac atcaaaacct tgttctcttc gcgttactgg
     1081 cggcgcacgg cgtttaacag cgtcttcttt gtctgcctcg taatcccatg gtttgtgatt
     1141 tatacctggc tgccaactat cgcccagact attggtctgg aagatgcgct gactgccagc
     1201 ctgatgctta atgcgttgtt aattgtgggc gcgctgctgg gattagttct gacgcacctg
     1261 ctggcacatc gcaaattttt gctgggaagt tttttgctgc tggcggcaac gctggtagta
     1321 atggcctgtt tgccttccgg cagttcatta acgctgctgc tttttgttct cttcagcacc
     1381 accatttcgg cagtcagtaa tctggtgggc attttgcctg cggaaagttt tcctactgac
     1441 attcgctcgc tgggagtcgg ttttgccacc gccatgagtc gacttggcgc ggcggtaagt
     1501 actggcctgc tgccgtgggt gctggcgcag tggggaatgc aagtcacctt attgctccta
     1561 gcgacagtgt tgttggttgg ttttgttgtg acctggctat gggcaccaga aactaaagcc
     1621 ctcccgctgg tggcggcggg aaatgtagga ggtgcgaatg aacattctgt tagcgtttaa
     1681 agccgaaccg gatgccggaa tgctggcgga aaaagagtgg caggcggcgg ctcagggtaa
     1741 tagcggaccg gatgtttcgc tactgcgaag tttactcggt gctgatgaac aggccgctgc
     1801 cgcgctgctg ttagcgcaga gaaaaaacgg tacgccgatg tctttaaccg cgttgagcat
     1861 gggggatgaa cgggcgttgc actggctgcg ctatctcatg gctcttgggt ttgaggaagc
     1921 tgtattgctg gagacggcgg cagatctgcg ctttgcgccg gaatttgtcg ctcgccatat
     1981 tgccgaatgg cagcgtcaga atccgctgga tctgataatt accggcagcc aaagcagcga
     2041 agggcagaat gggcaaacgc catttttgct ggcggaaatg ctgggctggc cctgcttcac
     2101 ccaggtggag cgtttcactc tcgacgcgct gtttatcacc ctcgaacagc gtactgaaca
     2161 tgggttgcgc tgttgccggg ttcgcttgcc tgcggtgatt gccgtgcgtc agtgcggtga
     2221 agtggccttg ccagtaccgg gaatgcgcca gcgaatggct gcggggaaag cagaaattat
     2281 ccgtaaaact gttgctgccg aaatgccagc gatgcagtgt ctgcaactgg caagagcaga
     2341 gcaacgacgc ggagcgacgc tgattgatgg gcaaacggta gcagaaaaag ctcaaaagct
     2401 atggcaggac tatttacgcc aaaggatgca gccatgaata tcgcaattgt gacaataaat
     2461 caggagaatg ccgcaatagc gagctggctg gcggcgcagg atttcagcgg ttgtacgctg
     2521 gcgcactggc aaatagaacc tcagcccgta gtggcggaac aggtgctgga tgcgatggtg
     2581 gaacagtggc agcgaacgcc tgcggacgtt gttctctttc cgccgggaac gtttggcgat
     2641 gaactgtcga cccggctggc ctggcgttta catggtgcaa gcatctgtca ggtgacttcg
     2701 ttagatattc caaccgtaag catgcgcaaa tcccactggg gaaatgcgtt aacggcgacg
     2761 ctgcaaacag aaaaacgccc gttatgcctt tccctggcgc gtcaggcagg agcagcgaaa
     2821 aacgccacgt tgccctctgg aatgcagcaa ctgaacattg tcccaggtgc gcttcccgac
     2881 tggctggtta gcacagagga tctgaaaaat gttagccgcg acccattagc tgaggccaga
     2941 cgggtgctgg tggtgggaca gggcggagag gcagataacc aggaaatcgt tatgctggca
     3001 gaaaagctgg gagccgaagt gggctacagt cgggcgcgag tgatgaacgg tggcgttgat
     3061 gctgaaaaag tgattggtat ttccggacat ctgctggcac ctgaggtgtg cattgttgtt
     3121 ggcgcttccg gcgcagcagc gttaatggcg ggcgtacgca atagcaagtt tatggtggcg
     3181 attaatcatg acgccagcgc agcggtattt tcacaggcag atattggcgt ggttgatgac
     3241 tggaaagtgg tgctggaggc actggtgaca aacattcacg cagaatacca ataacgtgct
     3301 gccggatgat cctgcctacg gttctggact ggggacctgt aggcctgata agacgcgtta
     3361 agcgtcgcat caggcgctga atgtcggatg tggcgtgcgg ccaacaaatc aaaacaaatt
     3421 caattggtta cgattatccc gtcaaagcaa ttttttcgct aacgtccaga ctccggtatt
     3481 cgtggtggaa agcgccacga cacccgcgtt aatcgcatta cgcgcatctt cttcatcgca
     3541 taccagcccg ccagcaatca gcggttggcg gattttttcc gtcacccagc ccagcacttt
     3601 gggcatgcag cctggcagga tctcaataca atccggattc gattgcgcaa cttacttatc
     3661 aatgttgtga aacgaaatcg aatcaacaat aaacaggtga tgaatacaga aaaaaacttc
     3721 cgctcttgcc gcttttagca tcgatgcttt ggtgctgata attccatccg cctcggtcac
     3781 cagtttcaaa aactgaatca ccacttcttt atttgacgcg ccttccagca aatcaacatg
     3841 aataaaagca tatttcccgg cgttcttaat ctttttaacg atattactga tggtgcagat
     3901 attgccgtac aacacggaaa taaactggca ttcagaatcg atggccagtt gcaggctggc
     3961 attatcttta acggcagcaa tcaccggatt ctggcggagc aggtgtaaga ggggcataaa
     4021 ttatccttat tatttatcca aagcggtagg tgatgccgaa tccggaaggc ggatagcgcc
     4081 actgttgtag tgtggattcg tcgcacagca aacggcaggt gccacactcc aggcagccgc
     4141 gatagtcaat acgtaagtca ccttcctgtg tgagagaaaa aagacctacc ggacaggcgt
     4201 taatcaaccg ttgtaccatc tgacgctcaa cggagtcagc cggaacaatg tgcggcgcat
     4261 cagcaacgcg ccagagatta cgggctacag acatcgcaga ctcctgataa catcgcccgc
     4321 cagatgccac aggccatgac gacgtaaatg atgccagaat aactggcgca gcggtggaac
     4381 aggtttatca ccctgatccc ataaatcgcg ggaaatatcc tgcattaacg caggccacgc
     4441 ccgatacctg ccagggcgtt gcaaaagcgc cggaacatgc tgataacgtt gtagaacatc
     4501 ccatagcagg ctgcgctcta cgttgtgatg ataaagcgca aacagatttt gcggctcgcg
     4561 gtgctggcag gcgcttatta gcgtttgtgc cgccgcctgt gcgccagtca gcgccatatc
     4621 catgccgcgc acggaaattc cggtattgac gcaactgcgc aacgcatcgc ccaccagcag
     4681 ccagccgtta ccggcgtatt gcaccggcat actgtgcaag ccaccttctg gcaccagatg
     4741 cgcaccatac tccagtgatt ccgtgttttt gataagcgtg cgcactgccg gatgcgcttt
     4801 aaagcgagtc agcagctcgc ttgccggaac acgactttgc gtaagggaag agagcgggca
     4861 aacaatccct aacgagagcg tttgttgatt agtataaaga aatgcgccgc cgggtaagtc
     4921 atcacagatt ccgccgctga acagcaacgc tgcgccttcg ttattctcca gatgaaaacg
     4981 ttcttcaata gcggatgttt ccagcgacag cacttctttg atccccaacg ccatcgcttc
     5041 gccagcagga cgagtcacta acccgtgacg ttcagccagg acgctgttgg caccttctgc
     5101 cagcaccaca taacgggcgc ggagaatatc gtcaccacaa ataacgccac agactctgcc
     5161 gttttcttca tacagtgcat ccaccgtcgc tccggggatg cattcgacac cttctttttc
     5221 ggcttcggca accagccacg gatcgaaccg tgcacgtaat acattccagg attcaccgcc
     5281 gggctgtaag ctggaaaacg tcgttgcgcc atccggcgtt aacagggaaa ggctttcgtg
     5341 agtgatgcgt cgttcaagag gcgcggtcag atgaaattgc gggaggagtt ccgcgagtgc
     5401 atgggtatat aaccgcctgc cggaaagatt tttgctgccg gggatttcag cgcgttcgag
     5461 taacaaaacg gataaatccg ctcgcgcgca gcgtaacgcg caagcggtgc ctgcaatacc
     5521 ggcaccaata ataataatgt cgcagtcgtc ttccatgtca tgccccagat atccagaaca
     5581 caacacccta acatagcgtt actaaaggga aattgaccgc cgacacactc ttatcattcg
     5641 ccgcgataga tacaccctgc ggtgcaggtt tcttttacca tcaccgcact taacagcggc
     5701 acaacgggtt taacctgatc ccaaatccat tttgctaaaa cttcgctggt tggattttcc
     5761 agacctggaa tatcattgag ataatggtga tcgaggcgct cataagttgg cttaaacgcc
     5821 gctttcagtt cagcgaaatc gataatccag cccgtatgcg gatcgacttc cccggtaatt
     5881 tccagtcgca ccataaagga atgcccgtgc aggcgaccac atttatgccc ttccgggaca
     5941 tgtggtaagc ggtgagcggc ttcgaaggtg aaatctttaa ataacgtggt ggacatcata
     6001 atttctctac aggacgataa aaccgccgta gagtaccgga aaggctgttt tttcgcatta
     6061 tctaaaacga ctttgttgcg caaaatcgct gatttatctt aatgattggc taaattcatt
     6121 tgtttttcat taggttggtt aatctatttt gttgttaaag actattgcta aaacaggtta
     6181 gtcgatttgg ttattagtta tcgctatccc gtctttaatc cacaccgttc accccgttaa
     6241 ccttaccttc tcttctgttt tatgggcgct gacagggcgc agaaacagct ttgcttactg
     6301 gaacataacg acgcatgacg acacaggtcc caccttccgc gttgcttccg ttgaacccgg
     6361 agcaactggc acgccttcag gcggccacga ccgatttaac gcctacccag cttgcctggg
     6421 tttctggcta tttctggggc gtgctcaacc agcagcctgt tgcgcttgca gtgacgccag
     6481 cgcccgccgc agaaatgccg ggtataacga ttatctctgc ttcgcaaacc ggcaatgcgc
     6541 gccgggttgc tgaagcatta cgcgatgatt tattagcagc aaaactgaac gttaagctgg
     6601 tgaacgcggg cgactataaa ttcaaacaaa tcgccagcga aaaactgctc atcgtagtga
     6661 cgtcaacgca aggggaaggg gaaccgccgg aagaagccgt cgcgctgcat aagttcctgt
     6721 tctccaaaaa agcgccaaag ctggaaaaca ccgcgtttgc cgtgtttagc ctcggcgata
     6781 gctcttatga atttttctgc cagtccggga aagattttga cagcaagctg gcggaactgg
     6841 ggggtgaacg cctgctcgac cgtgtcgatt ctgatgttga ataccaggct gctgccagcg
     6901 agtggcgcgc ccgcgtggtt gatgcgctta aatcgcgtgc gcctgtcgcg gcaccttcgc
     6961 aatccgtcgc tactggcgcg gtaaatgaaa tccacaccag cccgtacagc aaagacgcgc
     7021 cgctggtggc tagcctctct gttaaccaga aaattactgg gcgtaactct gaaaaagacg
     7081 ttcgccatat cgaaattgac ttaggcgact cgggcctgcg ttaccagccg ggtgacgcgc
     7141 tgggcgtctg gtatcagaac gatccggcac tggtgaaaga acttgtcgaa ctgctgtggc
     7201 tgacaggcga tgaacctgtc actgtcgagg gcaaaacgtt gcctctgaac gaagcgctac
     7261 agtggcactt cgaactgacc gtcaacaccg ccaacattgt tgagaattac gccacgctta
     7321 cccgcagtga aacactgctg ccgctggtgg gcgataaagc gaagttacag cattacgccg
     7381 cgacgacgct gattgttgac atggtgcgtt tctccccggc acagcttgat gccgaagcgc
     7441 taattaatct gctgcgcccg ctgaccccgc gtctctattc cattgcctcc tcgcaggcgg
     7501 aagtcgagaa cgaagtacac gtcaccgttg gtgtggtgcg ttacgacgtg gaaggccgcg
     7561 cccgtgccgg tggtgcctcc agcttcctcg ctgaccgcgt ggaagaagag ggcgaagtcc
     7621 gcgtatttat cgaacataac gataacttcc gcctgcccgc taacctggaa accccggtga
     7681 ttatgattgg cccaggcacc ggtattgcgc cgttccgcgc ctttatgcag caacgcgccg
     7741 ctgacgaagc gccaggtaaa aactggctgt tctttggcaa tccgcatttt acggaagact
     7801 tcctgtatca ggtggagtgg cagcgctacg tcaaagaggg tgtgctgacg cggatcgatc
     7861 tcgcctggtc gcgcgaccaa aaagaaaaag tttacgtaca agataaactg cgcgaacagg
     7921 gcgcggagct gtggcgctgg atcaatgacg gtgcccacat ttatgtctgc ggcgacgcta
     7981 atcgcatggc gaaagacgtt gagcaggcac ttctggaagt gattgccgaa tttggtggca
     8041 tggacaccga agcggcggat gaatttttaa gtgagctgcg cgtagagcgc cgttatcagc
     8101 gagatgtcta ctaatgagcg aaaaacatcc agggccttta gtggtcgaag gaaaactgac
     8161 agacgccgag cgcatgaagc ttgaaagcaa ctacctgcgc ggcaccattg cggaagattt
     8221 aaacgacggt ctgaccggcg gctttaaggg cgataacttc ctgctgatcc gcttccacgg
     8281 catgtatcag caggatgacc gcgacatccg cgccgaacgt gctgaacaga agctggagcc
     8341 gcgccacgcg atgctgttgc gctgccgtct gccgggtgga gttatcacca ccaaacagtg
     8401 gcaggcgatc gataaatttg ccggtgaaaa caccatctat ggcagcattc gcctgaccaa
     8461 ccgccagacg tttcagttcc acggcattct gaaaaagaac gtcaaaccgg tgcaccagat
     8521 gctgcactcg gtcggtcttg atgcgctggc gactgctaac gacatgaacc gtaacgtact
     8581 ctgcacctcg aacccttacg agtcgcagct gcacgcagaa gcgtacgagt gggcgaagaa
     8641 gatttctgaa catctgctgc cgcgtacccg cgcgtatgcg gagatctggc tcgatcagga
     8701 aaaagtcgcc accaccgatg aagaaccgat ccttggtcag acttacctgc cgcgtaagtt
     8761 caaaaccacg gtagtgatcc cgccgcagaa cgatattgat ctgcacgcca acgacatgaa
     8821 cttcgtggcg atcgcggaaa acggcaagct ggtgggcttc aacctgctgg tgggcggtgg
     8881 gctttctatc gaacacggta acaagaaaac ctacgcccgc acggcgagtg agtttggcta
     8941 tctgccgcta gagcatacgc tagcggtggc ggaagccgtc gtgacaactc agcgtgactg
     9001 gggtaaccga accgatcgta aaaatgccaa aaccaaatac acactggagc gcgtgggggt
     9061 tgagacgttt aaagcggaag tggagcgtcg cgcggggatc aaatttgaac cgatccgccc
     9121 gtatgaattt acaggacgcg gcgatcgtat tggctgggtt aagggcattg atgataactg
     9181 gcacctgacg ctgtttatcg aaaatggtcg catccttgat tatccggggc gtccgctgaa
     9241 aactggcctg ctggagatcg cgaagatcca caaaggtgat ttccgcatta cggcgaacca
     9301 gaatctgatc atcgccggtg tgccggaaag cgagaaagcg aagatcgaga aaattgccaa
     9361 agagagcggg ttaatgaatg ccgtcacgcc gcagcgtgaa aactcgatgg cctgcgtgtc
     9421 attcccgact tgcccgctgg cgatggcgga agcggagcgt ttcctgccgt cttttatcga
     9481 caacatcgat aatttaatgg tgaaacatgg tgtcagcgat gagcatatcg tgatgcgtgt
     9541 aacaggctgc ccgaacggtt gtggtcgcgc gatgctggcg gaagtgggcc tggtgggtaa
     9601 agcaccgggt cgctacaacc tgcatcttgg cggcaaccgc attgggacac gtatcccacg
     9661 gatgtataaa gaaaacatca ccgagccgga aatcctggcg tcgcttgatg aactgatagg
     9721 gcgctgggcg aaagagcgcg aagcgggtga aggcttcggc gactttacgg tgcgtgcggg
     9781 catcattcgc ccggtgctcg atccggcgcg cgatttgtgg gattaaccat cagcccggtc
     9841 ttgtaggcct gataagacgc gtgagcgtcg catcaggcaa ggcaaacagt gaggaatcta
     9901 tgtccaaact cgatctaaac gccctgaacg aactgccgaa ggtagatcgc attctggcgc
     9961 tggcagaaac taacgccgaa ctggaaaaac tggacgctga aggccgcgtt gcctgggcgc
    10021 tggataatct gcccggtgaa tatgtacttt cttccagctt tggcattcag gcggcggtga
    10081 gcctgcatct ggtgaatcag attcacccgg atattccggt gatcctcacc gacaccggct
    10141 atctgttccc ggaaacctac cgctttattg acgagttaac ggacaaactc aagctcaacc
    10201 tgaaagtgta ccgcgctacc gaaagcgccg cctggcagga agcacgctac ggcaaactgt
    10261 gggaacaggg cgttgaaggc attgaaaagt acaatgacat caacaaagtc gaaccgatga
    10321 accgggcgct gaaagaactg aatgcgcaaa cgtggtttgc tggcctgcgg cgtgaacaat
    10381 ccggcagccg tgccaattta ccggtgctgg caattcagcg tggcgtattt aaagtgctgc
    10441 cgattatcga ctgggataac cgaactattt atcagtacct gcaaaaacat ggcctgaaat
    10501 atcacccatt atgggacgaa ggatatttat cggtgggcga tacccataca acccgtaaat
    10561 gggaacccgg catggcggaa gaagaaacgc gtttctttgg cttaaaaagg gaatgtggat
    10621 tacacgaagg gtaaatattg ccggataaat taaacgcctc tgtcagaaat gatgggggcg
    10681 tttttatttt gcgagcagca ttccggcatt taaaggtgaa catcgcaggg aaaattaaca
    10741 ggtatagaat ggcgttgggt gcttgaggct gtctggcgtt aagcattcgc gaggttccag
    10801 atggacaaaa gccccaggcg atatttctat caacctgagg ctcgcgttcg aacgtagcaa
    10861 ttcggatgtt agtctcttct ttgcagggag gcaagacatg ctgacaaaat atgcccttgt
    10921 ggcaatcatc gtactgtgtt gtacagtact gggattcacg ttgatggtag gtgactcgtt
    10981 atgtgagttg agtatcagag aacgtggtat ggagtttaag gcagttctcg cttacgaatc
    11041 gaagaagtag ccaccacgcg ggggtaaaac cccgcgtact gaattgtgat ggtttggtct
    11101 cctgcaccct ttttttaaat ttatattttt tatgtgaata tatagtctct atttattatt
    11161 atgaatttca aatgaaaatc attaatttat tcataatgta aatgttttat tggctgatat
    11221 atatcataga tagtttttaa aatatgcgta aatatccttt aagtttactg aaggataaaa
    11281 atattgtgac tttctttgat ttctggggaa aaaaccgacg tggcgagaaa gagggtatcg
    11341 acgactatca ccttctttgc tggcatttgc tggatgtggc cgcaatgggc tatttaatgg
    11401 ttaaaagaaa ttgcttcggg ctggctgatt attttcgtca attagggatt tctgacaagg
    11461 aacaggcagc tcgatttttc gcctggttgc tgtgctggca cgatattgga aaatttgccc
    11521 gctcttttca acaactttac ctgccccctg aattcaagat tcaggaaggt gccagaaaaa
    11581 attacgaaaa aatctctcat tcaacgctgg gctactggtt gtggaatcat tatttaagtg
    11641 aatgtcagga gttacttcct tcatcttcgc tctctccgcg caaacttagg cgagtgatag
    11701 aaatgtggat gcccgtgacg acagggcatc atggtcggcc ccctgatcgt atggatgaat
    11761 tggataattt tctgcctgaa gacaaagctg cagcacgaga ttttctcctt gaaatcaagc
    11821 cactgtttcc gcgaatagag atccccacat tctgggatga tgacgagggc gttgaactta
    11881 taaaacaact ttcctggtat atctctgcaa ccgtcgtact tgcagactgg acagggtcgt
    11941 caacgcgatt ttttccacgc gtcgcacagg caatggatat tgaagattac tggcaaaagg
    12001 ctttagttca ggctcaaaac gcattaaccg tctttccgcc acaagcagaa accgcgcctt
    12061 tcaccggaat taatacgctg tttcctttta ttgagaatac gacaccgtta cagcaaaagg
    12121 tgctggatct ggatatcaat cagccaggac cacagttgtt tattctggaa gatgtgaccg
    12181 gcgcaggtaa aacagaagcg gcgcttatcc tggcgcacag gttgatggct gcgggtaacg
    12241 ctcaggggct gttttttggc ctgccaacaa tggcaacggc caatgccatg tacgatcgac
    12301 tggtcaaaac ctggcttgct ttctattcgc cagaggtgtt ccccgctcca gcggagataa
    12361 accggcgcaa tacggtgcgg ttttcccgga agtaaaccgg acgcactgga tgcgatgatg
    12421 ggtatcactt agaattcccc gcccctgcgg tagaactccc agctcccatt ttcaaaccca
    12481 tcaagacgcc ttcgccagct ccttcaccaa cggtagcatt atccgcataa cgtcacggca
    12541 gcgacgttct attcttccag gaagtgcctt atcaatatgt tgttgattat ccagtcttac
    12601 gtcatgccag ctatttcccg ccgggaaggc aggtgttttt gcgcgttgct gataaccatc
    12661 cttatttcca agattccagt tagtcgcttc caccgaaagt acagcaatgc ccgctttgtc
    12721 gaatatttct gcgtcattac aacccccagt gcctttcgga taatttttat tcaaacccgg
    12781 attggtcgtt gcggctattc cgtgactgcg tgcaattgcc agcgccctgt cgcgcgttaa
    12841 tttccttact gcttcagggg tttttacacc gctgttgaaa tacaatttat cgccaacaat
    12901 taagttatcg agattaatca ccagcagcgt atttttcttt tcggtgtcac tcatccgctt
    12961 gagtaaattc tcagcgccta attttccttc ctcttcgccg ctggtcgcca caaatcgaat
    13021 accatactcg gtaggcgtat ttttcaggcg ttctgccagt tccagcatga cacctaaacc
    13081 tgcggcgtta tcatctattc cttgtaacgt cagtccgccg agattggcat ctgcgtcagc
    13141 atcgctcagc ggggcgtaag tatccagatg cgccataatg atgatctgct gcggcgcttt
    13201 gccttcatga gcggcaatca ccgtacttcc tgtcacgtta tgccagttct tgcgattatc
    13261 gcgggcggta taaatatacc gactattaaa tgtccgaata tcactgcgat aacccatttg
    13321 ctgaaactgt tggcgaatat aatcggcaga taacatttct gctggagttc cggtcatgcg
    13381 tcccggaaag aaagtagcaa tatgtcgtgc ctgggtatta gcaaaatcgc caggtttagg
    13441 tgacgaggcg tgtacgggga gaataaagca tacgccgagc gccagggcag cggtacggtg
    13501 gcgcaatgcg gaaaacatag tgagtcctta aataccatgc aaattttttt accgccatag
    13561 tatgaaactg ccgctgcgct aaaacaattt caaatcttcc taaacgcccg aaatccggtg
    13621 ccttaagcac tttttgatat tagctttgcc aaatcgttat tccgttaagg aactactcat
    13681 tctaattggt aatttcattc gttctcttac gctccctata gtcgaaacat ctgatggcaa
    13741 gaaaatagcg gtattgcaaa ggaacggtta tggctcaaaa acgacttact cacctgcggc
    13801 aactggaggc ggaaagcatc cacattattc gcgaggtggc ggcagaattc tcaaatccgg
    13861 tgatgctcta ctctatcggt aaagattcca gcgtcatgct gcatctggcg cgcaaggcgt
    13921 tttatccagg tacgctgcct ttcccgttgc tgcatgtcga taccggctgg aaattccgcg
    13981 agatgtatga gttccgcgat cgtacggcga aagcctacgg ctgcgagctg ctggtgcata
    14041 aaaacccgga aggcgtggcg atggggatta atccattcgt gcacggcagc gcgaaacata
    14101 ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa caaatacggt tttgatgccg
    14161 ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc taaagagcga atctactctt
    14221 tccgtgaccg cttccatcgc tgggatccga aaaatcagcg cccggagctg tggcacaact
    14281 acaacgggca aattaacaaa ggcgaaagca tccgcgtctt cccgctctct aactggaccg
    14341 agcaggatat ctggcaatac atctggctgg aaaatatcga cattgtgccg ctgtatctcg
    14401 ctgcggaacg tccggttctg gaacgcgacg gtatgttgat gatgattgat gacaaccgta
    14461 tcgacctgca accgggcgaa gtgatcaaaa aacggatggt gcgtttccgt acgctgggct
    14521 gctggccgct gaccggtgcg gtggagtcaa atgcacaaac actgccggaa atcatcgaag
    14581 agatgctggt ttccaccacc agtgaacgtc agggtcgcgt gattgaccgc gaccaggcgg
    14641 gatccatgga gctgaaaaaa cgtcaggggt atttttaaga tgaacaccgt acttgcacaa
    14701 caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg cgcaacaaca taaaagcctg
    14761 ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa gtaccctgat tggtcgtctg
    14821 ctgcacgata cccgccaaat ctacgaagat cagctctcat cgctgcataa cgacagtaag
    14881 cgtcacggaa cccagggcga aaagctggat ctggctctgc tggtggacgg cctgcaagct
    14941 gagcgcgaac agggcatcac tattgatgtg gcctaccgct atttctctac cgagaagcgt
    15001 aaatttatta tcgccgacac cccagggcac gagcagtaca ctcgcaatat ggcgactggc
    15061 gcatcgacat gtgaactggc gatcttactg attgatgccc gtaaaggcgt gctcgatcaa
    15121 acccgtcgtc acagttttat ctccacactg ttggggatca aacatctggt cgtggcgatc
    15181 aacaaaatgg atctggtgga ttacagtgaa gagacgttca cccgtattcg tgaagattat
    15241 ctgacctttg ccgggcagct gccgggtaat ctggatatcc gctttgtgcc gctctccgca
    15301 ctggaaggtg acaacgtggc ttcgcaaagt gaaagtatgc cgtggtacag cggtctgaca
    15361 ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg tggatgccca gccaatgcgc
    15421 ttcccggtgc agtacgttaa ccgcccaaat ctcgattttc gtggctacgc cggaatgctg
    15481 gcatccggtc gcgtggaagt cgggcaacgt gtaaaagtgc tgccctctgg tgtggaatca
    15541 aacgtcgcgc ggatcgtgac ttttgatggt gatcgcgaag aagtctttgc cggagaagcg
    15601 atcaccctgg tgctgacgga tgagatcgat atcagccgtg gtgatctgct gctggcggca
    15661 gacgaagcgt taccggcggt gcagagtgcg tcggtggatg tggtatggat ggcggaacag
    15721 ccgctttccc cgggccagag ttatgacatc aaaattgccg gtaagaagac gcgtgctcgt
    15781 gttgatggca ttcgttatca ggttgatatt aataacctta cccaacgcga agttgaaaac
    15841 ctgccgctga acggcatcgg cctggtggat ctcacttttg acgagccact ggtgttagat
    15901 cgctatcagc aaaatccggt taccggtggg ctgattttta tcgatcgcct gagcaatgtg
    15961 accgtgggtg ccggtatggt gcacgagcca gttagccagg caacagctgc gtcatctgaa
    16021 ttcagtgcat tcgaactgga attgaatgcc ctggttcgtc gccactttcc gcactggggc
    16081 gcgcgcgatt tgctggggga taaataatgg cgctgcatga cgaaaacgtc gtctggcata
    16141 gtcatccggt cactgtgcaa caacgcgaac tacaccacgg tcatcgtggt gtagtgctgt
    16201 ggtttaccgg cctctccggg tccggtaaat caacggtcgc cggggcgctg gaggaggcgt
    16261 tacataaact cagcgtcagt acgtatctgc tggatggcga caatgttcgc cacggattat
    16321 gcagcgatct cggttttagc gatgccgatc gtaaagagaa tatccgtcgc gtcggtgaag
    16381 tggcgaattt gatggttgaa gccggactgg tggtgctgac cgcatttatc tcgccacacc
    16441 gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg gcgctttatt gaagtgtttg
    16501 tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa aggcttatat aagaaagcgc
    16561 gtgccggtga actgcgcaac tttacgggaa tagattccgt ttacgaagcg cctgaatcgg
    16621 cagaaattca tctcaatggt gaacaattag taacaaattt ggtacagcaa ttattagatc
    16681 tgttgagaca gaacgatatt atcagatcct gagacaccac cgggtttcca tgcccggttc
    16741 gtcaaagtaa caggatcaaa tatgcgtaat agccataaca ttacactaac aaataacgac
    16801 agccttactg aggatgaaga aactacatgg tcactgcctg gtgccgtggt cggttttatc
    16861 tcctggttat ttgcgctggc gatgccgatg ttgatttatg gctctaacac gctgttcttc
    16921 tttctctaca cctggccttt ctttctggca ctgatgcctg tcgcggtagt ggtggggatt
    16981 gcgctgcatt cattgatgga cggaaagcta cgctacagta ttgttttcac cctggtgaca
    17041 gtaggcatta tgtttggcgc actgtttatg tggctactgg gctaatttgt actttcttgt
    17101 ctcctctgtt cataattcaa accgtaacta ataatgagat tatgttctgc acgccctggg
    17161 tatacgtaac aatgggcaaa tgtggtacat ttgcccgcgt tgtcgcggta tccccaacag
    17221 aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc agtttttcag ggggcaggat
    17281 gggtaaacta acgctgctgt tgctggctat tctggtctgg ctacagtatt cgctgtggtt
    17341 cggtaagaac ggtatacatg actatacccg cgtcaatgat gatgtggcgg cactgcaagc
    17401 tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt gccgaaattg acgatctcaa
    17461 tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa ctcagcatga ccaggccggg
    17521 cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc gcacagtctg cggggcaaaa
    17581 caatcgataa atcagcccgg gaattaacat ggcaaccact catttggatg tttgcgccgt
    17641 ggttccggcg gccggatttg gccgtcgaat gcaaacggaa tgtcctaagc aatatctctc
    17701 aatcggtaat caaaccattc ttgaacactc ggtgcatgcg ctgctggcgc atccccgggt
    17761 gacacgtgtc gtcattgcca taagtcctgg cgatagccgt tttgcacaac ttcctctggc
    17821 gaatcatccg caaatcaccg ttgtagatgg cggtgatgag cgtgccgatt ccgtgctggc
    17881 aggtctgaaa gccgctggcg atgtgcagtg ggtattggtg catgacgccg ctcgtccttg
    17941 tttgcatcag gatgaccttg cgcgattgtt ggcgttgagc gaaaccagcc gcacgggagg
    18001 gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc gaaccgggca aaaatgccat
    18061 tgctcatacc gttgatcgca acggcttatg gcacgcgctg acgccgcaat ttttccctcg
    18121 tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa ggcgctacca ttaccgacga
    18181 agcctcggcg ctggaatatt gcggatttca tccgcagttg gtcgaaggcc gcgcggataa
    18241 cattaaagtc acgcgcccgg aagatttggc actggccgag ttttacctca cccgaaccat
    18301 ccatcaggag aatacataat gcgaattgga cacggttttg acgtacacgc ctttggcggt
    18361 gaaggcccaa ttatcattgg tggcgtacgc attccttacg aaaaaggatt gctggcgcat
    18421 tctgatggcg acgtggcgct tcatgcgttg accgatgcat tgcttggcgc ggcggcgctg
    18481 ggggatatcg gcaaactgtt cccggatacc gatccggcat ttaaaggtgc cgacagccgc
    18541 gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg gttataccct tggcaacgtc
    18601 gatgtcacta tcatcgctca ggcaccgaag atgttgccgc acattccaca aatgcgcgtg
    18661 tttattgccg aagatctcgg ctgccatatg gatgatgtta acgtgaaagc cactactacg
    18721 gaaaaacttg gatttaccgg acgtggggaa gggattgcct gtgaagcggt ggcgctactc
    18781 attaaggcaa caaaatgatt gagtttgata atctcactta cctccacggt aaaccgcaag
    18841 gcaccgggct gctgaaagcc aatccggaag actttgtggt ggtggaagat ttgggctttg
    18901 agcctgatgg tgaaggtgag catattctgg ttagaatcct caaaaacggc tgcaataccc
    18961 gttttgtggc ggatgcactg gcgaaattcc tgaaaattca ttcccgtgaa gtcagcttcg
    19021 ctgggcaaaa agacaaacat gctgtaacgg aacagtggtt atgtgctcgc gtgccgggca
    19081 aggaaatgcc cgatctgagc gcctttcaac tggaaggctg ccaggtgctg gagtatgcgc
    19141 ggcacaagcg caagctgcgt ttaggggcgc tgaaaggtaa cgcctttacc ctggttctgc
    19201 gcgaagtgag caatcgcgat gacgttgaac aacgtctgat cgatatttgc gtaaaaggtg
    19261 taccgaacta cttcggtgcc caacgttttg ggattggcgg tagcaacttg cagggcgcgc
    19321 tgcgctgggc gcaaaccaat actccggtgc gcgatcgcaa taaacggagt ttttggttgt
    19381 cggcagcccg cagtgcgttg tttaatcaga ttgttgctga gcgcctcaaa aaagcagacg
    19441 ttaatcaagt tgttgacggc gatgcgctac aattagccgg acgtggtagc tggtttgtag
    19501 caaccaccga agaactggcg gaattacagc gtcgcgtcaa cgataaagag ctgatgataa
    19561 ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg tgaagcgctg gcattcgaac
    19621 aagcagctgt cgccgcagaa actgaattac aagctttact ggtgcgcgaa aaagttgaag
    19681 ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag ctggaactgg tgggatgacg
    19741 tcaccgtaga gatccgtttc tggcttccgg cgggtagttt tgcaaccagc gttgtcaggg
    19801 aacttatcaa cacaacaggt gattatgcgc atattgctga gtaattatga cggggtacat
    19861 gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt ttgctgacgt tcaggtggtc
    19921 gcccccgatc gtaaccgcag cggcgcttca aattctctga cactggaatc ctccctgcgc
    19981 acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg gaaccccgac cgattgtgtc
    20041 tatcttggcg tgaatgctct gatgcgtccg cgcccggaca ttgttgtgtc cggaattaac
    20101 gccgggccga atctggggga tgatgttatt tattccggta cggtagccgc cgcgatggaa
    20161 ggccgtcatt taggttttcc ggcgctggcc gtctcgcttg acgggcataa acattacgac
    20221 actgccgcgg cggtaacctg ttcaattttg cgcgcactgt gtaaagagcc gctgcgcacc
    20281 ggacgtattc ttaatattaa cgttcaggat ttacccttgg atcaaatcaa aggtattcgc
    20341 gtgacgcgct gcggtacacg acatccggca gatcaggtga tcccgcagca agatccgcgc
    20401 ggcaatacgc tgtactggat tggcccgccg ggcggtaaat gtgatgccgg tccggggacc
    20461 gattttgctg cggtagatga gggctatgtc tccatcacgc cgctgcatgt ggatttaact
    20521 gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca gcgtgggagt tggcacgcaa
    20581 tggtaagcag acgcgtacaa gcacttctgg atcaattacg tgcgcaaggt attcaggatg
    20641 agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa attcgttgat gaagcgtttg
    20701 aacaaaaagc ctgggacaat atcgccttgc cgataggtca ggggcagaca atttcgcagc
    20761 cgtatatggt ggcgcgaatg accgaattac tcgagctgac gccgcagtcg cgggtgctgg
    20821 aaattggcac tggttcggga tatcaaacgg caatcctggc gcatcttgtc cagcatgttt
    20881 gctcggttga acggattaaa ggcttgcagt ggcaggcacg tcgccgcctg aaaaatcttg
    20941 atttacataa tgtttcaacc cgtcatggtg atggatggca aggttggcag gcacgtgcgc
    21001 cgtttgacgc tatcattgtt acggcggcac cgccggaaat tccaactgcg ctaatgacgc
    21061 agctggacga aggcgggatt ctcgtcttac ccgtagggga ggagcaccag tatttgaaac
    21121 gggtgcgtcg tcggggaggc gaatttatta tcgataccgt ggaggccgtg cgctttgtcc
    21181 ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata cctggatttt tcctggttat
    21241 tttgccgtag gtcagcgtat cgtgaacatc ttttccagtg ttcagtaggg tgccttgcac
    21301 ggtaattatg tcactggtta ttaaccaatt tttcctgggg gataaatgag cgcgggaagc
    21361 ccaaaattca ccgttcgccg cattgcggct ttgtcactgg tttcgctatg gctggcaggc
    21421 tgttctgaca cttcaaatcc accggcaccg gtcagctccg ttaatggcaa tgcgcctgca
    21481 aatactaatt ctggtatgtt gattacgccg ccgccgaaaa tggggacgac gtctacagcg
    21541 cagcaaccgc aaattcagcc ggtacagcag ccacaaattc aggctactca acaaccgcaa
    21601 atccagccag tgcagccagt agctcagcag ccggtacaga tggaaaacgg acgcatcgtc
    21661 tataaccgtc agtatgggaa cattccgaaa ggcagttata gcggcagtac ctataccgtg
    21721 aaaaaaggcg acacactttt ctatatcgcc tggattactg gcaacgattt ccgtgacctt
    21781 gctcagcgca acaatattca ggcaccatac gcgctgaacg ttggtcagac cttgcaggtg
    21841 ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca ttacccaggc cgacgcagca
    21901 gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg ttgctgttgc gtcgcaaccg
    21961 acaattacgt attctgagtc ttcgggtgaa cagagtgcta acaaaatgtt gccgaacaac
    22021 aagccaactg cgaccacggt cacagcgcct gtaacggtac caacagcaag cacaaccgag
    22081 ccgactgtca gcagtacatc aaccagtacg cctatctcca cctggcgctg gccgactgag
    22141 ggcaaagtga tcgaaacctt tggcgcttct gaggggggca acaaggggat tgatatcgca
    22201 ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc gcgttgttta tgctggtaac
    22261 gcgctgcgcg gctacggtaa tctgattatc atcaaacata atgatgatta cctgagtgcc
    22321 tacgcccata acgacacaat gctggtccgg gaacaacaag aagtgaaggc ggggcaaaaa
    22381 atagcaacca tgggtagcac cggaaccagt tcaacacgct tgcattttga aattcgttac
    22441 aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc gataaatcgg cggaaccagg
    22501 cttttgcttg aatgttccgt caagggatca cgggtaggag ccaccttatg agtcagaata
    22561 cgctgaaagt tcatgattta aatgaagatg cggaatttga tgagaacgga gttgaggttt
    22621 ttgacgaaaa ggccttagta gaagaggaac ccagtgataa cgatttggcc gaagaggaac
    22681 tgttatcgca gggagccaca cagcgtgtgt tggacgcgac tcagctttac cttggtgaga
    22741 ttggttattc accactgtta acggccgaag aagaagttta ttttgcgcgt cgcgcactgc
    22801 gtggagatgt cgcctctcgc cgccggatga tcgagagtaa cttgcgtctg gtggtaaaaa
    22861 ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga ccttatcgaa gagggcaact
    22921 tggggctgat ccgcgcggta gagaagtttg acccggaacg tggtttccgc ttctcaacat
    22981 acgcaacctg gtggattcgc cagacgattg aacgggcgat tatgaaccaa acccgtacta
    23041 ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta cctgcgaacc gcacgtgagt
    23101 tgtcccataa gctggaccat gaaccaagtg cggaagagat cgcagagcaa ctggataagc
    23161 cagttgatga cgtcagccgt atgcttcgtc ttaacgagcg cattacctcg gtagacaccc
    23221 cgctgggtgg tgattccgaa aaagcgttgc tggacatcct ggccgatgaa aaagagaacg
    23281 gtccggaaga taccacgcaa gatgacgata tgaagcagag catcgtcaaa tggctgttcg
    23341 agctgaacgc caaacagcgt gaagtactgg cacgtcgatt cggtttgctg gggtatgaag
    23401 cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac ccgtgaacgt gttcgccaga
    23461 ttcaggttga aggcctgcgc cgtttgcgcg aaatcctgca aacgcagggg ctgaatatcg
    23521 aagcgctgtt ccgcaagtaa gtaagcatct gtcagaaagg ccagtctcaa gcgaggctgg
    23581 cctttttctt ttgtttggga agcttgtgtt tttgtcgcct ggataagaca cgtcagcgtc
    23641 ctacccggca atcagcattc tatgtgctca tcatcttgcg caccaactgc ataaacagct
    23701 cctgctcttc tccactcaaa cgccccaaaa attcctcatc cacgctgtca ccaataggta
    23761 tcgccgcagc aagtagcttt tctccttcgg cagtcagcca gacaaagcgt cgccgcttgt
    23821 cagcgacgtc atgttcacgt ctgactaagc cacgattttc cattcttgcc aacatttctg
    23881 ctagcgttgc tttggtgctg actgctgctt ctatcagtgc cacctgttca ataccaggtt
    23941 tatcagcaat ggcgcgcatc accgcgtact gtggtttggt caggtcaggt aactcgtgct
    24001 gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg atggaacgct ttatttcgta
    24061 acgccatgta aactccgggc taattctctg tcgctatcat agcggttttt tagtgacgaa
    24121 gaagatgttg ttacttttca atccttgccg ctgtggaaat gaaggagtat gttaataata
    24181 acgttcgtat acgaataatt aagagaataa gcaatgaaac tgatcgtcgg gatgacaggg
    24241 gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc tgcgtgagat gccgaatgtc
    24301 gagactcatc tggtgatgtc gaagtgggcg aaaaccacca ttgaactgga aacgccttac
    24361 agcgctcgcg atgttgctgc cctcgcagac ttcagccata acccggcgga tcaggcggcg
    24421 atcatctcat ccggttcttt tcgtaccgac ggcatgatcg ttattccgtg cagtatgaaa
    24481 acgctcgccg gtatccgcgc tggttacgcc gatggcctgg tagggcgcgc ggcggacgtc
    24541 gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg aaatgccgct tagcaccatc
    24601 catctcgaaa atatgctcgc actttcacgc atgggcgtgg cgatggtgcc gccgatgcct
    24661 gccttttata accatcccga aacggtagat gacattgtcc accatgtggt agcccgcgtg
    24721 ctggatcaat ttggcctcga acatccccac gccaggcgct ggcaaggatt gccgcaggcc
    24781 cggaattttt ctcaggagaa tgaataatgg catttgatga tttacgcagc tttttacagg
    24841 cgcttgatga ccacggccag ttactgaaaa tcagcgaaga agtgaacgct gagccggatc
    24901 tggcagcagc agctaacgcc accgggcgta tcggcgacgg cgcgcccgcg ctgtggtttg
    24961 ataatattcg cggctttacc gatgcccgcg tggcgatgaa taccatcggt tcctggcaga
    25021 accacgcgat ttcccttggc ctgccaccaa acaccccggt caaaaagcag attgatgagt
    25081 ttatccgccg ctgggataac ttcccgattg ccccggagcg ccgcgccaat ccagcctggg
    25141 cgcagaacac cgtcgatggc gaagagatca acctgttcga tattctgccg ctgtttcgcc
    25201 tgaatgatgg cgatggcggt ttctatctcg acaaagcgtg cgtggtttcc cgcgatccgc
    25261 tcgacccgga taacttcggc aagcagaacg tcggcatcta ccgcatggaa gtgaagggca
    25321 agcgtaagct cggcctgcaa ccggtgccga tgcacgatat cgccctgcat ctgcataaag
    25381 cagaagagcg cggtgaagat ctgccgattg cgatcacgct cggtaacgat ccgatcatca
    25441 cgctgatggg ggccacgccg ctgaaatacg atcagtctga gtatgaaatg gcaggcgcgc
    25501 tgcgtgaaag cccgtacccg atcgccaccg ctccgctgac cggttttgat gtgccgtggg
    25561 gttcagaagt gatcctcgaa ggggttatcg aaagccgtaa acgcgaaatc gaagggccgt
    25621 tcggtgagtt taccgggcac tactccggtg ggcgcaacat gaccgtggtg cgcatcgata
    25681 aagtctctta tcgcagcaaa ccgattttcg aatcactcta tctcggtatg ccgtggaccg
    25741 aaatcgacta cctatgacta ggtaatttat tcaagataac cgggaagaga ttaaaaaaga
    25801 cgggaataga ttggaatcaa caagttagga actcaaggtt ttcaagataa agcgagtcca
    25861 aaaatgttaa acggttcgat atcaataaaa cattcaagat aactcacagg ttcttacaat
    25921 agcctttaaa gaccgttcaa aaacgattca ggtgtttatt atagcgctga tgggtgcaag
    25981 cgccaaaacc aatgtaattt tagcacagct aggcacttgc actgggttat gcgctggctg
    26041 ctttctgagg tagatttaag tgatcgccct tagcttcgga caacgcgtaa atctctctaa
    26101 ccttttcgtc tggcaaatta ccaaggctta acgctatggc tctacgaact ccaactagag
    26161 cgtgcaactc ttggatgtgc tcatccagaa gaatgagtaa ttgctccgct ccttttctcc
    26221 caagaacatc agctacttgt tgtttttctt gtggtttaag aaatgacaag aacattagaa
    26281 tttgagcatc aacattatcc gcattctctt ttttgggttc tttggaatct tttagttcaa
    26341 catcaccctc tcccgtcatg agccattcca aggtaacatt ttcgctttct gctatctttt
    26401 ttaactgata cgggcgtggt atcggtgcgt aatcccgcct gttgtaataa ttttttaaag
    26461 tgccttcatt gatcccccat gcccgagcag ccgcatttct tgaagggtat cgttctacta
    26521 acctaaagat tctttcgata atcggttctt ttccatcggc aggaaaagaa ccttggggtg
    26581 cttttctcat tcttggttcc tttctatcta attcattgat aaataataaa attaaaaaat
    26641 aaaccataaa aacaccaaag aaccctttgc agggaatctt tggtatctta atctttatcc
    26701 atacggatat ccgaccggat atccgcgcgg gtaattttgt aggttaaagg aaagatgaga
    26761 caaagagaag atagtgaccg cgattggcct tcgctgaaga ttcagctggc gctcaaagat
    26821 aacgggtggt cttccttccg ggagctttca ctccatgctg gttatgctca ggattcactg
    26881 aaagcggtag ttcgcacacc aaacaaaaaa tacgaacgcg taatttctga cgctataggt
    26941 gtaccgccag aaatcatctg gcccagccgg tatcgtaacg atggatttat caggaaggcg
    27001 tcgtgatatg cagtcgtacg ttaccgtaaa tgacctgctc ggcgtaccag gaatgccagc
    27061 caccacaaaa ggcatccgac aggcgttaca gcgtttttct cgtgatcttg gtgacgttag
    27121 tcgccgtcgg gaaggcacaa aagccattga gtaccacatt gattgtctgc ctgaaataac
    27181 acgaaaggca ctgcgtgaac gctatgtgga acagctggtg gctacagaga acaatgtttc
    27241 tgaagtgaaa gctgttacca gaaaaacacg caatcctgac gctgtccagg caatcgaagc
    27301 atatcgcggt tctccacaac tgatggaaga acgcctgaat gcgctgaccg aaaaccagcg
    27361 ctgggtatct gaagcaagag ctgcgctggt ggtggaagtg ctgaagctgg aaagcgccgg
    27421 taaccccggg cgactgaaag ccattaactt tcttgttgaa aaagcccgta aaggtgagct
    27481 gccggagcgc ctgcaacagg ccgcagttaa cgccaatgca aaacgtggcg ctaatcgtac
    27541 tatcagccgc gatccacttt accagtgggt tctgaaatac aaccagtcgc agaacgccgc
    27601 cgaacggctg ctgttactgg caccgggaaa acgcgacgaa ataaaaccgg aagaaatcag
    27661 ctggctgcct gaatttctgg cgcagtatcg ccaggtgaac ggcagaccca tgtcagaagc
    27721 ctacgaggat tttgtcgcag aatggcagcg tcgccatgcg gacgaaccgt acatgctgga
    27781 ggtgatgcct tcctatgatg ttgtccggta tgccatgaaa aaactgccgg aagtggtgaa
    27841 acaaaaaggg cgcgttaccg gcagtgaata ccgtcagctt gaaggattca cccgccgcga
    27901 ctggacagct atgccggtga attatgtctg gattggtgac ggtcacggta tgaagctgaa
    27961 atgtgcgcat ccgatccacg ggcgtccatt ctcaccggaa gtcacgtttg tgatcgatgg
    28021 tggaacacgg tttgttgttg gctggagtct tgaccttgcc gaaaacgtgt tcgccgttgc
    28081 cggtgcgata cagcacggca ttcgcaacca cggaaaaccg ttcctgtatt actcggataa
    28141 cggttccggt gaaacggctg acatgctgga caaagaggtc gtgggtattc tgccacgcct
    28201 ggggattaaa caccctaccg gtattgccgg taatccacag ggacgcggca ttattgaacg
    28261 cctgaaccgg acactgccga tgcgtatagc caggaggtat cgcacctatt ttggcaaggg
    28321 tgctgaccgg gaatcgttgc gtgtacttaa ccgtgatctg cgttcagcgt ttaacgccct
    28381 gcaacaggac aagcctctga acgaccgcca gaaagcggca atgcgcgagc tgccatcgtg
    28441 ggcggagctt attgaggcta ttcgtgaagg cgtggaatgg tacaacaacc gcccgcattc
    28501 tgagctgccg atgaaaccga acggacggca ttacagcccg acagagttca gaaaaaaacg
    28561 ccaggcagaa gaagacaccg aaattgagtg gctgagtgat cttgagctgc gcgacatgtt
    28621 ccgcccaatg gtggaacgac cggtaagacg ctgcgaaatt cagtggctga acaatattta
    28681 ctacgcgccg gagttgcgtg acgaacacgg tcgcaaagtg cttatcagtt atgacattca
    28741 tgacgccgag cgaattacgg tacgtcgcaa ggacggcagc tttatctgtg aggcgatatg
    28801 gaacggcaat aaacgcgcag cctttgctgt cagtgctgaa tatcacaaac aacagcagcg
    28861 tattaaagga atgcgcaaac gcgctgagga aaaaattcgt gatgctgaag atgaaggtat
    28921 tcagattctg gaacacaaac aggctgaacc ctggctgagt aatgtttatc gcccggttgg
    28981 taat
//
LOCUS       DAJTRW010000048        28914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRW010000048 DAJTRW010000000
VERSION     DAJTRW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO326_002247"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO326_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5966616.1"
                     /translation="MAFMLSPLLKRYTWNSTWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO326_002248"
     CDS             complement(2138..2584)
                     /locus_tag="OO326_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5966617.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO326_002249"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO326_002249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5966618.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO326_002250"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO326_002250"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5966619.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO326_002251"
     CDS             complement(5347..6009)
                     /locus_tag="OO326_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5966620.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO326_002252"
     CDS             6182..6595
                     /locus_tag="OO326_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5966621.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO326_002253"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO326_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5966622.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO326_002254"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO326_002254"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5966623.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO326_002255"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO326_002255"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5966624.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO326_002256"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO326_002256"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5966625.1"
                     /translation="MLIFEGKEIETDTEGYLKESSPWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO326_002257"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO326_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5966626.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO326_002258"
     tRNA            complement(9861..9948)
                     /locus_tag="OO326_002258"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO326_002259"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO326_002259"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5966627.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO326_002260"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO326_002260"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5966628.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO326_002261"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO326_002261"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5966629.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO326_002262"
     CDS             14005..14592
                     /locus_tag="OO326_002262"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5966630.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO326_002263"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO326_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5966631.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO326_002264"
     CDS             14984..15841
                     /locus_tag="OO326_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5966632.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO326_002265"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO326_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5966633.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO326_002266"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO326_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5966634.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO326_002267"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO326_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5966635.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO326_002268"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO326_002268"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5966636.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO326_002269"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO326_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO326_002270"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO326_002270"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5966637.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO326_002271"
     CDS             complement(22884..24023)
                     /locus_tag="OO326_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5966638.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO326_002272"
     CDS             complement(24069..26165)
                     /locus_tag="OO326_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5966639.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO326_002273"
     CDS             complement(26165..26911)
                     /locus_tag="OO326_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5966640.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO326_002274"
     CDS             complement(26908..27552)
                     /locus_tag="OO326_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5966641.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO326_002275"
     CDS             complement(27659..27964)
                     /locus_tag="OO326_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966642.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO326_002276"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO326_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5966643.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7319 c         7601 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggt gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccacg ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAJTRW010000049        28457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRW010000049 DAJTRW010000000
VERSION     DAJTRW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..431
                     /locus_tag="OO326_002277"
     CDS             <1..431
                     /locus_tag="OO326_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966644.1"
                     /translation="VSGESEFAKKFQGKALPFDVRIISASQNEDWLVIATKVLPGADL
                     RTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEAGHVLGYLDDDYDSSSPYVG
                     DISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVTK"
     gene            428..814
                     /locus_tag="OO326_002278"
     CDS             428..814
                     /locus_tag="OO326_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966645.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            839..3052
                     /gene="tssI"
                     /locus_tag="OO326_002279"
     CDS             839..3052
                     /gene="tssI"
                     /locus_tag="OO326_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5966646.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3075..3519
                     /locus_tag="OO326_002280"
                     /pseudo
     CDS             3075..3519
                     /locus_tag="OO326_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3541..3948
                     /locus_tag="OO326_002281"
     CDS             3541..3948
                     /locus_tag="OO326_002281"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5966647.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            3945..6233
                     /locus_tag="OO326_002282"
     CDS             3945..6233
                     /locus_tag="OO326_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5966648.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6230..7219
                     /locus_tag="OO326_002283"
     CDS             6230..7219
                     /locus_tag="OO326_002283"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966649.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7319..7522
                     /locus_tag="OO326_002284"
     CDS             7319..7522
                     /locus_tag="OO326_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966650.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7540..7860
                     /locus_tag="OO326_002285"
     CDS             7540..7860
                     /locus_tag="OO326_002285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966651.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(7926..8158)
                     /locus_tag="OO326_002286"
                     /pseudo
     CDS             complement(7926..8158)
                     /locus_tag="OO326_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8268..9038)
                     /gene="yafV"
                     /locus_tag="OO326_002287"
     CDS             complement(8268..9038)
                     /gene="yafV"
                     /locus_tag="OO326_002287"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5966652.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9219..9665
                     /gene="ivy"
                     /locus_tag="OO326_002288"
     CDS             9219..9665
                     /gene="ivy"
                     /locus_tag="OO326_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5966653.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9708..12152)
                     /gene="fadE"
                     /locus_tag="OO326_002289"
     CDS             complement(9708..12152)
                     /gene="fadE"
                     /locus_tag="OO326_002289"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5966654.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12392..12970
                     /gene="lpcA"
                     /locus_tag="OO326_002290"
     CDS             12392..12970
                     /gene="lpcA"
                     /locus_tag="OO326_002290"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5966655.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13176..13943
                     /locus_tag="OO326_002291"
     CDS             13176..13943
                     /locus_tag="OO326_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5966656.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13914..14654)
                     /locus_tag="OO326_002292"
     CDS             complement(13914..14654)
                     /locus_tag="OO326_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5966657.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15038..15559
                     /locus_tag="OO326_002293"
                     /pseudo
     CDS             15038..15559
                     /locus_tag="OO326_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15735..16232
                     /gene="rayT"
                     /locus_tag="OO326_002294"
     CDS             15735..16232
                     /gene="rayT"
                     /locus_tag="OO326_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5966658.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16449..18161)
                     /locus_tag="OO326_002295"
                     /pseudo
     CDS             complement(16449..18161)
                     /locus_tag="OO326_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18133..18918
                     /gene="lafU"
                     /locus_tag="OO326_002296"
     CDS             18133..18918
                     /gene="lafU"
                     /locus_tag="OO326_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5966659.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            18989..20044
                     /gene="dinB"
                     /locus_tag="OO326_002297"
     CDS             18989..20044
                     /gene="dinB"
                     /locus_tag="OO326_002297"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5966660.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20096..20389
                     /gene="yafN"
                     /locus_tag="OO326_002298"
     CDS             20096..20389
                     /gene="yafN"
                     /locus_tag="OO326_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5966661.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20392..20790
                     /gene="yafO"
                     /locus_tag="OO326_002299"
     CDS             20392..20790
                     /gene="yafO"
                     /locus_tag="OO326_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5966662.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20800..21252
                     /locus_tag="OO326_002300"
     CDS             20800..21252
                     /locus_tag="OO326_002300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5966663.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21498..22541
                     /locus_tag="OO326_002301"
     CDS             21498..22541
                     /locus_tag="OO326_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5966664.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22603..23217
                     /locus_tag="OO326_002302"
                     /pseudo
     CDS             22603..23217
                     /locus_tag="OO326_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23274..24731)
                     /gene="pepD"
                     /locus_tag="OO326_002303"
     CDS             complement(23274..24731)
                     /gene="pepD"
                     /locus_tag="OO326_002303"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5966665.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            24993..25451
                     /gene="gpt"
                     /locus_tag="OO326_002304"
     CDS             24993..25451
                     /gene="gpt"
                     /locus_tag="OO326_002304"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5966666.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25543..26787
                     /gene="frsA"
                     /locus_tag="OO326_002305"
     CDS             25543..26787
                     /gene="frsA"
                     /locus_tag="OO326_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5966667.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26845..27246
                     /gene="crl"
                     /locus_tag="OO326_002306"
     CDS             26845..27246
                     /gene="crl"
                     /locus_tag="OO326_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5966668.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27285..28340)
                     /gene="phoE"
                     /locus_tag="OO326_002307"
     CDS             complement(27285..28340)
                     /gene="phoE"
                     /locus_tag="OO326_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5966669.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7006 a         7100 c         7557 g         6794 t
ORIGIN      
        1 ctgtttccgg tgaatctgag ttcgctaaaa aatttcaggg taaagctttg ccttttgatg
       61 taagaataat ttctgcatct cagaatgaag actggttggt tattgcaaca aaggttttgc
      121 ctggtgctga tttaagaaca tatgttgatt ttaaaaacag tacggttcat gttgattcag
      181 cgggcctgga aaaagtagcg aaatgtatta actgtaataa tacgttgcaa gtaaatatac
      241 ctcatgaagc cggacatgta cttggttact tagatgatga ttatgattct tcgtctccgt
      301 atgttggaga tattagtggg ttgatgaatg tgggaatgga gctttgggaa cgatacctta
      361 aaaacgcaac tataacgttg aatatcataa tgcctgaaac taaatttact ttattgaatg
      421 taaccaaatg aaatttttct gtattatagt gatttttctt tcaataaatt acatagctat
      481 agtgaatgtt ttcaagtctt ctgcagttgc tcaatccacc aaatattatg aagaaaatga
      541 tatacatgat ggaagtaatg acatcttttt tatgaagaaa tatgggccgc ctgctcatga
      601 gttcgtgagg ccacgatata aatttgttgg tgagtattat atcggcttga atcaaactta
      661 tcgcataaca gatcctgaat ttcaagatgt tcctattaag gaaatgttct ggaatttgca
      721 gggggatttg aatctgacat gttggtttca ttataaagac gggcaatgga gagtgattac
      781 ttatattttt tggccaccag gggctagatt ttaatccggt attaaaggag caaatatcat
      841 gagttttgta tctacgggta ataaaccagt tggaaacggt ggacccgtca tcaccacacc
      901 accaatagct ggcgaaagtg gcggtatgag cacaggttca gctgtaaccg atgtatctgg
      961 tgccgccgag gagatggcag aacaggctgc ggcggacctg tttggtgcgc tgccggaacc
     1021 gtcaggtctg gttaaagccg cagtagctgc tgcgcaggct gccgctgcag cagcagggat
     1081 ctcggatatg gctggtgcag ttcaggatgc agcggcgtcg cttgcagccg gtgctccggg
     1141 ggcgcataac gtcacagtaa gtggtagtgc ggtgccgccg cagatgcttt tgtttgcagg
     1201 catgaatggt tcggaagggc ttgggaatct gttttcttac acagtacagc ttaaaacgct
     1261 ggacgccctg aacctggggt acgtttcccc cgctgctaac ctgccgcttc agctcatggt
     1321 gggcaaagac ctgtgcgtca gtatcgaact ggacggcggc ggaaaacggt atatcagcgg
     1381 gctggtgacg gcggcgaggg tggtcggcca tgagagccgt tcggtaacgt atgaattacg
     1441 cattgagcca tggctgaagc tgctgaccca taccagcgac tacaaagcgt tccagaacaa
     1501 aaccgtggtg gatattctcg atgaagtact ggatgaatat tcctttccgg tggaaaagcg
     1561 gctggtggaa aattatccac cccgcgcctg gcaggtgcag tatggtgaaa ccgacttcga
     1621 tttcatccag cgcctgatgc aggaatgggg gatttactgg tggtttgagc acagcgagaa
     1681 cagccacacg ctggtgctgg tggatgccat caatggtcat aaagcgtgcc ctgactcgcc
     1741 gctggtggag tggcaccagg aagggctgaa actggataag gaatttatcc acaccattac
     1801 ggccagtgag aggctgcgta ctggaaagtg ggtgatggac gattttgatt ttatgaagcc
     1861 tcgttcgctg cttaaaagta ccgtggccag tccgcgtgat accggtcatg ctgaatacga
     1921 gcattacgaa tggcctggtg attacttcac tacgggcgaa ggcgagatgc tgacgcgcat
     1981 tcgtatggaa gcgcagcgca gcccaggcag ccgggcacat ggggccggtc atatccgtac
     2041 cctcatgacc gggtacacct ttacgttgat gaatcacccg acggcagaaa ttaatcagga
     2101 atatctgctg gtacagacca ctctgttttt acgggataat gcgcagcaca gcggtcagga
     2161 tcagcatttt acctatgtca ccacgtttga actgcacccc accagcgaag tttaccgccc
     2221 acaacgaacc ctgagcaaac cccacaccaa aggcccgcag agcgccatcg tcaccggccc
     2281 ggcggggcag gaaatctgga cggataaata tggccgggtg aaggtgcagt ttggctggga
     2341 ccgttacgga aagaatgacg aaaacagttc ctgctggatc cgcgtcagct atccgtgggc
     2401 gggtaaagga ttcggcatga tccagatccc gcgtattggc caggaagtac tggtggattt
     2461 caaaaacggc gatccggatt tgcccatcat cgtggggcgc acctataacc aggacaccat
     2521 gccgccgtgg gggctgccgg gcatggcgtc gcagagtggg atattcagcc actcattgca
     2581 gggagggccg actaacggaa atatgctgcg cttcgatgat aaaacgggtg cggaagaggt
     2641 gaagttccat gcggaaaaag acctcaatac cacggtaaaa aacaatgaaa cccacacggt
     2701 catggtcgat cgcataaaaa ccattgtcaa aaatgaaacc atcagcgtgg gagaagaccg
     2761 taatgccacc atcacaaaaa atgatggtct ttccgtcaaa ctggctcaaa ccatcaatgt
     2821 cggaacgaca tatcgtctgg atgttggcga tcaatttacc cttcgttgcg gcaatgcagc
     2881 gcttgtatta cacaaggatg gctcgattga gttttgcgga aaacaactgt tactgcacac
     2941 cagcgatgtt atgcaactca ttggcagtgg cattgatatg aatccggatg gcggcacagc
     3001 catgactgcg gatgatattg ccccgttacc tacccccgac gtttctgaat aaaaaatata
     3061 aaaggaattt ttctatgacc agcctttacc gtattcagga gggctgcttt gccctgcctg
     3121 aaacttttct tgatcgcacc gtcaatatct tcgtcccatc gggcaatgaa agggcaactc
     3181 ccagtctgaa tattttccgc gatacgctca ggccagatga aaatctgacc acctatattg
     3241 accgccagat agccttgatg aaaaaaaaac ctaggccagc accgggtact ttcgcgggga
     3301 acggcactgg cgggaactgg cgaagctgcc atgacaggtg aacagattct cgcaactcat
     3361 cgttctggaa aaacagaagt gtaccagcgt caggccgggt ttatcaccgg gccagcaaag
     3421 gtactaatgt tgacgctgac cactcagcgt ccttttgatg atcacaccga ccagctatgg
     3481 actgcatggt tgactagttt tcagcctgct aaaagctaaa aaaaacaggg aggttttgtc
     3541 atgtatgaag ccgcacgggt ggatgacccc atctaccaca ccagcgccct tgccgggttt
     3601 ttgatagggg ccatcatagg tatcgccatt attgccgtcg ccagctttgc tttttttacc
     3661 tgcggctttt tggccgggct gattttgagt tttctggctg atcagatagc ttccggagtc
     3721 ctgcaactgg gtgaggctat tgggcgctcg atacattcca cagtgggaaa aattctgacc
     3781 ggttctgata atgtcagcac caacagtcgc ccggccgccc gggccgtact gagtacggtg
     3841 atatgcgaag accattcccc ggaaatccgc attgcccaag gttccggcaa catctacatc
     3901 aacagccagc tgcccgtaag gatgaccaca ctgagtgtga tgcagtgatt gaggacggct
     3961 cccccaatgt ctttctcggc ggcggtacgc agacggtact ggaaatcagc ccggaaatcc
     4021 ctgactggct gcgtcaggtg gtggatgtgc tgtatgtggt cgccggaatg cttggcgggc
     4081 tggctggcgc ctggcgtcag gcggcaaaga tggggtcgaa atttggtaca aaatgtgcgg
     4141 caaaatttat tggtggcgaa atggtcggga tggccatctc tgacacagtc atgggattgt
     4201 tcagcaatcc ggtagatgtc accactgggc aaaaaatttt gctgccggag acggatttta
     4261 ccctgccagg acgcctgcct gtcacctgtt cgcgctttta cgccagccac ctggaaactg
     4321 aaggattgct gggcagagga tggcggctga actgggagat aaacctgcgt gaggatgaaa
     4381 catatatcac cttcatcggt gtacagggtc gggaactgag ttatccgaaa gagatgctga
     4441 taccgggcca ccagatattc gacccggaag agcagtttta cctgagccgt ctgcatgacg
     4501 gatgctatgt gctgcattac accgactgca gctattacgt gtttgatgaa tttgatgacc
     4561 acggcgtcgc gccgttactt tttatggaga cgccgtaccg gcagcgtatt gctttcgggc
     4621 gtgagaacgg caggctggtg cgggtggcct ccagcagcgg acaccatctg ttattgcacc
     4681 gcacgatgac gcaagcaggg gaacgcctgt cgcatattga acttttgaag ggcgggcgtc
     4741 ccggtaacct ggtggaatat cgttacgacg ataacgggca actgactggc gtggtgaacc
     4801 gggctggtgt gacggtgcgc cagtttgcct atgaaaatgg cctgatgaca gagcatcgca
     4861 acgcgacggg ctttacctgt acctaccact gggaagagat tgaaggtttc ccgcgcgtgg
     4921 tggaacacac gaccagcgac ggcgaggatt accggttcca ctatgatttt gctggcgggc
     4981 agacggtggt aaccggcagg ccggagcaga agtggcagtg gtggtttgat gaagagacgt
     5041 atgtgactgc gcaccggacg ccgggcggcg gaatgtaccg cttcacgtac aacgaaaacc
     5101 attttccggt agcggtggaa ctccccggcg aacgcagggt aacgctggaa tacgacaccc
     5161 tgagccgggt ggtgaaagag acggacccgg caggccgggt gacacagacg cagtggaacg
     5221 gcagttttgc tgaaatcacg cgccgggcgc tggatgacga ccacgtatgg aaggcggatt
     5281 acaacgaaca cggccaggtg atacgggaga cggacccgga agggcgcgtc acgcggtacg
     5341 ggtatgatga tcaggggctg ccggagacgg tctgccaccc cggtaaacag caggaccgtt
     5401 acacctggaa cgcgctgggg ttactgagca gccaccgtcg catcacgggc agtgtgcagt
     5461 cctggcagta tacgcagcgc gggatgctgg cgcgccatac ggatgaagag aaacgggaga
     5521 cgcgctggca gtacaccccg gaagggctgg ttgccagtct gagcaacggc aacggggctc
     5581 agtaccggtt cagctacgac ggcgacggac ggctgacggg agaacagcgc ccggacggcc
     5641 tgataaggat gtttgcgctg aatgcggacg ggtttccggt catcatcccg acgcagggca
     5701 cagagggcgg ggtgcgcaat gaacagcagg agcgtgatgc gctgggccgc ctgctgcgca
     5761 gtgacacgca gcacagtaca cggaccttca gctacaaccg tcttgaccag ataacggaag
     5821 tgacgctcac ccccacggaa gaaggggaac ggctgcacca catgcaggcg gacacggtgc
     5881 gttttgcgta tgaccgcagc gggtggctga cggcagagca cagcgtgcac ggcagtatca
     5941 aataccggcg cgatgcgctg ggcaatccca ccgatatcac gttgccggac gggcagcatc
     6001 tcagtcatct ttactacggc agcgggcatc tgctccagac ggcactggac ggtatcacgg
     6061 tcagtgaata tgagcgtgac agcctgcacc gtcaggtgat acgcacgcag gggaaactgg
     6121 cgacgttcag cggctacaac gccgacaacc ggttaagctg gcagcgcagc ctgccaggtg
     6181 gcagccagaa tccacagcag gcggtactcc cccggcgcag aacgacagcg tgacggcgcg
     6241 ggactatatc tggaacgcag acggtgaagt cggcggcatc aatgacaaac tgcgcggttg
     6301 cctggtgttc agctatgacc gcagcggctg gctgaccagc cgcacggggc agatgtatga
     6361 ccatgaccat tattattacg acaaagcggg caacctgctg acggatgagt atcagggagc
     6421 ggtgatggat aaccgcctgc cgggttacgg gcgtgaccgc taccgctata acgaatgggg
     6481 cgaactgaca gagcgcaggg accagcagct ggagtggaac gcgcagggcc agttaacgcg
     6541 ggtcatcagc agcaacagcg agacgcggta tcagtacgat gcgctgggca ggcgcatcag
     6601 caaggcgacc agcaatctcc ataccgaccg gggagaacga agccggacaa cgtttgtgtg
     6661 ggaagggttc cggctgttac aggagacaac gtggcaggga aagcggacat acctgtacga
     6721 tgcggaacag ccgtacacgc cggtggctgc catcacgggg cgcggagaga gccagaaaat
     6781 ctggtattac cacacggacc tgacgggcac ggtacacgag gtaaccgcgc cggacgggac
     6841 gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac cggggcgaca tcagcaacag
     6901 cggggcgtac tttgagcagc cgcagcggct gccggggcag tactttgacg aagaaacagg
     6961 gctgcattac aatctgttca ggtattatgc gccggagtgc gggaggttcg taagccagga
     7021 cccgatagga ttaaacggtg ggttgaactt atatgcttat gcgcctaatc cgttaggttg
     7081 gatcgatccg ctaggactga attctttagg aatagattca aataatatat ttagaggaga
     7141 tggtaactat aaaggtggcg gaattggaca gccaaagagt ggcacaatat cagcacagga
     7201 cattattgat catctctaag aaaaaggtga tgggcgttta acatctttta gtactaaaaa
     7261 aacatctagt tccaactctc gtggtgcaga ttttttctca aaaaaacatg tcttcaaagt
     7321 ggcagtttct gatctcgatg atttagttaa aaataatcag atcactgttt taacacaaga
     7381 aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa attagaaaag aacttggtaa
     7441 tattcttcag aatatggcta ggaataatga ggtgttagtt catgggcaaa ttcttggaaa
     7501 taaagttagc cgatgtaatt agttagtgga gaggaagttg tggacgaaat tagaataaga
     7561 attgctggta atacggctgc gccttgtttc catgtgctaa aaaataaggg atataaagta
     7621 acaatggaaa ctcatgcatt atctgacgat tgtttagatt gtgtatatga ttggtatgcg
     7681 gaaaaaaatg gacaattttt tagtgcaacg agtccagaag agttacttgg attagttgca
     7741 atgtgggaag ttcgtggtaa tgactggaga gctaatttga atgagataaa tacttatcaa
     7801 aaagccatag aagaatcccc gttttttgat aaaaacggca atgaaataga gtctgactaa
     7861 attttattga tatagcaacg ataataaccg gaaatttctt ccagatcttt ccggcttttt
     7921 attatttaac tttcgttcag cacactaaac atcccgtctc gcatcccctt aactggctgt
     7981 ttaacctgat aaagttccct gcgcagttcc tgtacttcgt tgttatccca agcaggatca
     8041 aacatccctc cgagatcttc acggtaacgc ccgtgccgat ctcaaatccc gcttttctca
     8101 gccatttggc gctgattttg atcaccggcg tgtcgctgcg gttgggcaga taacccacgg
     8161 tacagcgacg ctgtgttttg gaaatggtga cttctggcgt aatattgtgt ttaagttagg
     8221 ggctttcgta atctagccca gcccccggcc ctaaacacaa cccccactca ccacaaccta
     8281 aactcatccg catcccgcca tgccggaaac ttttctctat attcccgcaa tgccaccatc
     8341 gacagctccg catcaatacg cgttgcctta tgcgcgtcgg cggtagcgat aatctcgcct
     8401 tgcggattaa tcacccggct gtcaccgcga taatggcagc cgttgccatc gctgccgacg
     8461 cgattgcatc ccgccacata cgcctgattc tcaatcgcgc gggccgtcag caatgcctgc
     8521 cagtgcagag agcgcggagc tggccagttg gcgacgtaca gggcgaggtc ataatcgttg
     8581 agattgcgcg accacactgg aaaacgtaag tcgtagcaca ccagcggcaa aatacgccag
     8641 ccgcgccatt ccacaatcac tcgcgcattg cccgctttat aatgcagatg ctcatctgcc
     8701 atgcggaaca gatgacgctt atcataaaaa tgtaccgtgc cgcccggctc aaccagcaaa
     8761 aagcggttaa ccgaaccaga ctccgtttgt aatgcaacac tgcctgcaat cagcgcattg
     8821 cactgctgcg ccttagctgt catccagttc actacgtcat cttgtgcaag cgacgaagct
     8881 gccgcttcca tggcaaagcc gctggtaaac atctccggta gaacgatcac atcgcgcccg
     8941 gtaatacctt ccagttgacg atcaaaatgg cgcaggttgg caggaccatc cacccacacc
     9001 aacggttgct gcaaaagcgt aatcttcaaa ctaggcacgg tgtacaactc ctttatgcga
     9061 agggttttat aactttaaca ccttatcagg cagttgcctt agcgcagaat aaattgataa
     9121 caaatgctga tattggaaat atctgatttg caaattatcg tgttatcgct aggctttagg
     9181 aggttaataa catgggcagg ataagctcgg gaggaatgat gtttaaggca ataacgacag
     9241 tcgccgcact ggtcatcgcc accagtgcaa tggcgcagga tgatttaacc attagcagcc
     9301 ttgcaaaggg cgaaaccacc aaagctgcgt ttaatcagat ggtgcaaggg cataagctac
     9361 ctgcatgggt gatgaaaggc ggtacttata ctcccgcaca aaccgtaacg ttgggagatg
     9421 agacgtatca ggtgatgagc gcgtgcaaac cgcatgactg tggctcgcaa cgtatcgctg
     9481 tgatgtggtc cgagaaatct aatcagatga cggggctgtt ctcggctatt gatgagaaaa
     9541 cgtcgcaaga gaaactcacc tggctgaatg tgaacgatgc gctttcgatt gatggtaaaa
     9601 cggtgctgtt cgcggcgttg accggcagcc tggaaaacca tccggatggc tttaatttta
     9661 aataattagc ggataaagaa acggagcctt tcggctccgt tattcaatta cgcggcttca
     9721 actttccgca ctttctccgg caactttacc ggcttcgtcg ccagctcttc cggatcaaag
     9781 tcatcaacgt taatactgcg cagacggctt tcttccgctt tcaccagaat agcggcttca
     9841 tctttatcaa tcagcccctt cgtcagcgcg ttgtgcgcca gttcatccag acgggtaaat
     9901 ggcagatttt tacccagctc tttacagatc cgctgatgaa tcgggtcggc ggcaatcaca
     9961 tccaccaacg cctcttccag taaaccaacc gggttatgct cgcttggcgt caggtactga
    10021 ccgcgaccaa tacgtgaacg agtggcgttc ggcacttgta aaatcttcgc cattttatga
    10081 tccagcttgt cagacggtgc cagataatga cgtccggtcg ggaagatcac cacattcagc
    10141 agcccggcaa ccacgcggtt cgggaagttt tgcagtaaat tgtccattgc ctgttctgcc
    10201 tgatacagcg catcctgtac gccccagtgc accagcggca gatcggcttc attacggcct
    10261 tcgtcgtcat aacgcttcag aacggcagag gcgaggtaaa gctggcttaa aatatccccc
    10321 agacgtgccg agatacgctc acgacgtttc aggctgccgc ccagcagtgc catcgagaca
    10381 tcagaaagca gggcgaggtt ggcgctcagg cggttcaggt gctgatagta gcgtttagtg
    10441 gcatcgccgg ttggcgtgct gctggttaaa ccgcgcgtca ggcccagcca gaagctgcga
    10501 actttgttgc taccgacgtg accgatatgt ttgaacaaca gtttatcgaa cgcgttgacg
    10561 tcattgttct tcgccgcttc catctcttcc agcacgtacg gatggcaacg aatcgctcct
    10621 tgtccgaaga tcatcatgct gcgggtcaga atgttagccc cttcaacggt gatggcaatc
    10681 ggtgcgccct ggtaagcacg cgccaggaag ttgctttgcc cgagcataat gcctttaccg
    10741 ccggtaatat ccatcgcatc aataatcgac tgctgcccgc ggtgggtaca gtgatactta
    10801 acgatagccg acagcacggc aggtttttcg ccgagcataa tgccgtaggt aatcagcgat
    10861 gccgcagcat ccattacata agcgttcccg gcaatacgcg ccagcggctc ttcaatccct
    10921 tccatcttac cgatagagat tttgaactga cggcgaatgt gagaatacgc gcccgttgcc
    10981 agcgctaccg atttcacgcc gccggttgag ttggaaggca gggtgatgcc gcggcctacc
    11041 gagaggcact ccaccagcat ccgccagcct tgcccggcca ttttcggccc gccgatgatg
    11101 taatcgatcg gcacgaagac atctttaccg cgcgtcggtc cgttctggaa cggtacgttc
    11161 agcgggaagt ggcgacgacc aatttccacg cccggcgtgg tggttgggat cagcgcacag
    11221 gtaatgccta aatcttctgc accgccgagt aatttttccg ggtcggagag tttaaacgcc
    11281 agcccaagca cggtcgcaat cggtgccagc gtaatgtagc gtttgttcca ggtcagacgc
    11341 atccccagca cctgctggcc ctgccattcg cccatgcaga caatcccggt gtccggaatc
    11401 gcgcccgcat cggaacccgc ttccgggctg gtcagtgcaa agcaggggat ctcctgacca
    11461 cgcgccagac gcggcagata gtgatctttc tgctcgtcag tgccgtaatg ttgcaacagt
    11521 tcgcccgggc ctaatgagtt tggcacgccg acggtaatcg ccaggatccc gctcacgcca
    11581 gagagttttt gcagcacacg agactgggca taagctgaga actccagtcc gccgtactct
    11641 tttttgataa tcatcgcgaa gaagcgatgc tctttaaggt acgcccacaa ctccggtggc
    11701 agatccgcca gctcatgagt aatctggaaa tcattcgcca tccggcaggc ttcttctacc
    11761 gggccgtcga gaaacgcttg ctcttcagcg gtcaggcgcg gctgcggata gttatgcagc
    11821 tttttccagt ccggcttgcc ctggaacaag tcgccctccc accaggtggt gcccgcatca
    11881 atcgcttctt tctcagtgcg cgacatcggc ggcatcacct tacggaaacc gcgaaatacc
    11941 ggcgcggaaa tcatcgactt acgcataggc gcaaagttaa atggcacgag gataatggtc
    12001 agaggcacca gtacccacgc cgaccacaga ccagcaacgc cgagggcggc tgtccaggcg
    12061 agcaaaatca gactgctgat aaataagctc acgcggtgat agaacaacgc gccgagcagg
    12121 acaaccgtag cgagaatact caaaatcatc ataacgaaaa gccccttact tgtaggaggt
    12181 ctgaccactt gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa tgtgtttaca
    12241 atataattac aacaaagctc acattgttgc tgtttttatc cgcacttcag gtcaaaaagt
    12301 cctggtcata gcacctgccc gtacttctcg cttttggcgg tatccggtac actgcatttt
    12361 gtctattaca tttatgctga aggatatcct catgtaccag gatcttattc gtaacgaact
    12421 gaacgaagcg gcggaaacgc tggctaactt tttaaaagat gacgccaata ttcacgccat
    12481 tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag tgctttcctg
    12541 cggcaacggc ggttcccatt gcgacgctat gcactttgcc gaagagttga ccggtcgcta
    12601 ccgtgaaaac cgtccgggct acccggcgat tgctatttct gacgttagtc atatttcctg
    12661 cgtcggtaat gatttcggtt tcaatgatat tttctcccgc tacgttgaag cggtaggtcg
    12721 cgaaggcgat gtactgctgg ggatctccac ctccggtaac tctgcaaacg tgatcaaagc
    12781 gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc ctgaccggta aagacggcgg
    12841 taaaatggct ggcacggcgg atatcgaaat tcgcgttccg cactttggtt atgccgaccg
    12901 cattcaggag attcacatta aagtgatcca tatcctgatc cagttaattg aaaaagagat
    12961 ggttaagtaa gtctggcgta ggccggataa ggcgtttacg ccgcatccgg cacttgtgcg
    13021 ctgatgcctg atgcgatgct gacgcgtctt atcatgccta caaatctgta cccgaaccgt
    13081 aggccgaata attcgtttac gccgcatccg acctgaaaat tcttaaatca atcttcgccg
    13141 gggtccatgc gctcccgctg ttgtggaggt tacccatgtg cgaattgctc gggatgagcg
    13201 ccaacgtccc taccgatatc tgctttagtt tcaccggact tgtacagcgt ggtggtggaa
    13261 ccgggccaca taaagatggc tggggcatta ccttttacga aggtaaaggc tgtcgcacat
    13321 ttaaagatcc acaacccagc tttaattccc ccatcgccaa acttgtccag gactacccga
    13381 taaaatcctg ttcggtggtg gctcatattc gccaggctaa tcggggcgag gtggcgctgg
    13441 aaaatactca cccatttacc cgcgagttat gggggcgtaa ctggacttat gcccataacg
    13501 gacaactgac gggctacaaa tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg
    13561 acagcgaaaa agccttttgc tggctgctgc ataaattaac gcagcgttac ccgcgcacgc
    13621 cgggcaacat ggcggcagtg tttaaatata tcgcctcact ggcggatgaa ctgcgggcga
    13681 agggcgtttt caacatgctg ctttcggacg ggcgctatgt aatggcgtat tgctcgacta
    13741 atttacactg gatcacccgc cgcgcgccgt ttggcgtggc aacgttgctg gatcaggatg
    13801 tggaaatcga cttcagctcg cagaccacac cgaatgatgt ggtcacggtg attgcgacac
    13861 agccgctgac gggcaatgaa acctggcaaa aaattatgcc aggcgaatgg cgcttatttt
    13921 gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca ccacttcgtg gcttaacggc
    13981 ttgctgacca cgtaacggcc attggccaca gaaacggtcg gtggcttacg ggtttgctca
    14041 aagtagtcgt agcccggctt cagctgctcc cagaagtcct taaagttgga atatttatgg
    14101 cgcttcatat tggcgtcggt catgcggaat gggtaaatac tcacctgcac gctcggctga
    14161 ccaaacacca gcgcaccagt aacgaactgg aagatctcat caataccctg attggtcatt
    14221 gcgtagcagc cgatggatac acaatcgccg tggatcatca ggtatttccc ttcataacca
    14281 tgcgcacggt cataagcatt ggggaatcca atattaatcg ctttgtagta acggctgtct
    14341 ggttttaact gattacgctg gacgctataa aacccttccg ggcttttgaa atcgccctga
    14401 cgctgttttg gccctaagcc gccggaatat ttacagattt tataactgtc gagcagttga
    14461 tattgctcgc ccattttgac gtaaagatcg agcgtacgtt cttccttgaa gatctggatg
    14521 tagacagggg atcccatcaa ctgctgctta tactctttgc tcactggcgt ggtcgagcta
    14581 ctgctgccca gcaaaccggc aaacgaaacg cacgggatca acaacatcgc aagaattaat
    14641 gcgattttac gcatactgct tattccttga taaaacggtt acacacgcca ggacggcaaa
    14701 atggatccca aatcggaata gtctggattt ggaaggctca cattatcacc aaaagagttt
    14761 tacgcaagcc tgtcggcgct gggtttacaa atttagcaca ggggcagttt tcaatagtcc
    14821 gaaaagcgca tgtatcattt atttcctaat attatgaaat ctcaatagta atatgcaaat
    14881 aagcaattaa taatcccgtt tactcctccg gtcttttaaa attctcacct tcattcagcc
    14941 ggtttatgtc tttcatgtct tcgttcttgc tcggcagatt tttgcatcca ggcgtgtttt
    15001 cgttatgcgt tttgctccct ttactcgcca gcgccaccac aaccgggagc agataaaaca
    15061 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggcgctggc
    15121 ggaaaacaac cactttttaa gcagcgatca cctgcataac atcaccgaag tggcgattca
    15181 ccgcctggag cagcagcttg gcaagcctta cgtctggggc ggcacgcggc ccgatcaagg
    15241 cttcgattgt agtgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
    15301 gcgcacggcc aatgagatgt accactaccg tcgggcaacg attgtggcga ataacgacct
    15361 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
    15421 ggtgtatctg ggcgacggtc agtttattga gtcactgcgc accggggaaa ccattcgggt
    15481 aagccggtta gccgaacctt tctggcagga ccatttttta ggcgcgcgca ggattttgac
    15541 ggaagagatg attttgtagg ccggataagg cgtttacgcc gcatccggcg gttgtgcacg
    15601 gatgcctgat gcgacgctga cgcgtcttat catgcctaca aatctgtgcg tttttgcgag
    15661 ccgctttccc gatataacac agcgtcattg tcggcctttc cttttcctct taccgttgag
    15721 aaaaaggagt cgctatgtcg gaatatcgtc gttattacat caagggggga acatggtttt
    15781 tcacggtaaa tttacgcaat cgtcgaagcc atcttctgac cacccagttc cagacgctcc
    15841 gtaacgccat tattaacgtt aagcgagaca gaccttttga aattaacgcc tgggtcgttt
    15901 tgccggagca tatgcattgc atatggacat tacccgaaag cgatgatgat ttttcctctc
    15961 gctggcggga gattaaaaag cagttcaccc atgcttgcgg attgaaaaat atctggcaac
    16021 cgcgtttttg ggagcatgcc attcgcaata caaaagatta tcgccatcat gttgattata
    16081 tttatataaa tccagagaaa catggctggg taaagcaggt gagtgattgg ccattctcaa
    16141 cgttccatcg cgatgtcgcg cgagggttat atcccatcga ttgggcggga gacataacgg
    16201 atttaagtgc cggggagcgt atcattttat aactgtgcgc tggtgccgga tgcgcttacg
    16261 cttatccggc ctacaaatcg tgctttaacg gtaggccgga taaggcgttc acgccgcatc
    16321 cggcagttat gcgcagatgc ctgatgcgac gctggcgcgt cttatcatgc ctaaaaactt
    16381 gtgcctgaac ggtaggccgg ataaggcgtt tacgccgcat ccggcaattg tgcaccgatg
    16441 ccggattctt acatcaacgc ccccataatc ttcaactcca gctcatccgg cacttcgtta
    16501 tacgacagca catgcagccc cggcgcaaac aaccttgcat aacgcggcag caaagggcgt
    16561 agctgcggcg gcaccagcag caccggatct ttccccgccg ctttcatctg ctctttcacc
    16621 tgtggcatcg tgctctggaa ctggttgagc atattcgggt caaccggcac gctgtcgagc
    16681 atcactttgc cggcctgttg cgcctgattc actacgttgg tcagcagatt ttccagctca
    16741 ttattcagcg tatacaccgt cagctcctgc ttgcgaacga acggatgagt aatgctgcgc
    16801 cgcagcgcca ggcgcacatc ggccgccagc agaatatgat ctttagtcac cgtgctactg
    16861 gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg aaacgccttc ggtcagcagc
    16921 gcccggtaca ctttcagcaa ttggctgtaa ttgagcgccg cgcttaaatc ctccgccagg
    16981 cgcggtgcca tcgacgacaa acggttatgc aactgcgtga tgtcatcata gttaaacaaa
    17041 tcaggaatat agctgcgcac aatcttgttc acatgggtgg caatcacact ggcgctgtcg
    17101 atcacctgat accccatatt cagagccttc gctttctgcg caggctggat ccaggtgacc
    17161 ggtatcccgt acgccgggtc gttccccagc acgccgtcaa tctcgccgta ggtttcgctg
    17221 gagggcagcg ccatcagttt atccgccgga atatccgctt catcagcctc aatgccgttg
    17281 atgaaaatag cgtactggct gggcttgagg cggaagtttt cccgaatgcg gatctccggc
    17341 agcagcacgc cgttaccgtc ggaaatcacc tgccgaacgc cgcgaatccg ctgggtgagc
    17401 gggttgccct gcgctttgtc caccagcgcc accagcttgt agccgaggct taagctgatg
    17461 ggctcgatca gcggaatagt ttcccaactg acctgttgtt cgctggtttc agtgatggtg
    17521 cgggtcagcg tttcgaggct tttctcttcc gcctccgccg cctgcgggcg tttgctcatc
    17581 cgccagccgg taaagccaag cagggcgctg aacagcaaaa acggcagatg cggcatcccc
    17641 ggcaccaccg ccagcacgaa catgatcccg gtggcggtgt agagcaccga cgggcttgcc
    17701 agcaactggt ggcgcacgtc atgggcgata tcgccgttgt cgctgacgcg ggtgacgata
    17761 atcgccgccg cggtggagag cagcagggaa gggatctgcg ccaccaggcc gtcgccgatg
    17821 gtcatcagca cgtattgctg gaaggcggcg tcggcgctca ggttgtattt gaagatgccg
    17881 atacagacgc cgccgctcag gttgatcgcc agaatcatca tcccggcgat ggcgtctccg
    17941 cgtacaaact tcgatgcccc gtccatcgcg ccgtagaaat cggcctcgct ggcaacatct
    18001 ttacgccggg tttgcgcctg cgcctggttg atcaatccgg cgttaagatc ggcgtcaatc
    18061 gccatctgtt tgccgggcat cgcgtccagg gtaaagcggg cagaaacctc ggaaatacgc
    18121 tcggcctctt tggtgacgac gataaagttg attgtcaaca gcgtcagtaa atccgagcgt
    18181 gaaagcatta tcgccgcgct gcacgggcag tcgattttta acggtggtgg gctgtcgccg
    18241 ttaaataaaa tcagcccgtc tcatccgcca aaacctgcca ccgtggcggt gccggaagag
    18301 acggagaaaa aagcgcgtga tgtgaatgag aaaacggcgc tgctgaagaa gaaatccgcc
    18361 accgagcttg gcgagttggc aaccagtatc aacaccattg cccgtgatgc gcatatggaa
    18421 gcgaatctgg agatggagat tgttccccag ggattacgcg tgctgattaa agacgaccag
    18481 aatcgcaata tgttcgaacg cggcagcgcg cagattatgc cgttctttaa aactttgctg
    18541 gtggagctcg cgccagtgtt cgactcgctc tataacaaaa ttattattac cgggcatacc
    18601 gatgcgatgg cctacaaaaa caatatctac aacaactgga acctttcggg cgatcgcgcg
    18661 ctttcagctc gtcgggtgct ggaagaggcc ggaatgccgg aagataaagt gatgcaggta
    18721 agcgcaatgg cggaccagat gctgctggac gcgaagaatc cgcaaagcgc gggcaatcgg
    18781 cgtattgaga ttatggtgct gaccaaaagt gcgtccgata cgctgtatca atactttggt
    18841 cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc tggataagca gcaggtgctt
    18901 tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc tcaaaacctg aaatcactgt
    18961 atactttacc agtgttgaga ggtgagcaat gcgtaaaatc attcatgtgg atatggactg
    19021 ctttttcgca gcggtggaga tgcgcgacaa tcccgctctg cgcgatatcc ctattgctat
    19081 tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc aattatcccg cgcgtaaatt
    19141 tggcgtacgt agcgctatgc ctactgggat ggcgctcaaa ttatgcccac atctcacctt
    19201 gcttccgggg cgctttgacg cctacaaaga agcctcaaat catatccgtg aaatcttctc
    19261 gcgctacacc tcgcgcattg aaccgttgtc actggatgag gcttatctcg atgtcaccga
    19321 tagcgtccat tgccacggtt ctgcgaccct catcgcccag gaaatccgcc agacgatctt
    19381 caacgagctg caactgacgg catctgcggg cgtggcacca gtaaagtttc tcgccaaaat
    19441 cgcctccgac atgaataaac ccaacggtca gtttgtgatt acgccggcag aagttccggc
    19501 atttttacaa accttaccac tggaaaaaat ccccggcgtc ggcaaagtct cggcggcaaa
    19561 actggaagcg atggggctgc ggacctgcgg tgatgtacaa aagtgtgatc tggtgattct
    19621 gcttaaacgc tttggcaaat ttggccgcat tttgtgggag cgtagtcagg ggattgacga
    19681 gcgcgacgtt aacagcgaac ggttacgaaa atccgtcggc gtggaacgca cgatggcgga
    19741 agatattcat cactggtctg aatgtgaagc gattatcgag cggctgtatc cggaacttga
    19801 acgccgtctg gcaaaggtaa aacctgattt actgattgct cgccaggggg tgaaattaaa
    19861 gttcgacgat tttcagcaga ccacccagga gcacgtctgg ccgcggctga ataaagctga
    19921 cttaatcgcc accgcgcgta aaacctggga tgaacgccgc ggcgggcgcg gtgtgcgtct
    19981 ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa agacaactgg tgctgggatt
    20041 atgatgtata ctattatgta tattctggtg tgcattatta tgagggtatc actgtatgca
    20101 tcgaattctc gctgaaaaat cggtcaatat cactgagtta cgtaaaaacc cagctaaata
    20161 ctttattgat caaccagttg cggttctttc taataatcgc cccgcaggat atctcttaag
    20221 tgccagcgca ttcgaagcgt taatggacat gcttgctgaa caagaggaga aaaagcccat
    20281 aaaggcgcgc tttcgtccaa gtgctgcaag attagaggaa attacacgcc gcgctaaaca
    20341 atatcttaat gatatgacgg atgatgattt caatgacttt aaggaataag gatgcgggta
    20401 ttcaaaacaa aacttattcg cctgcaactt acagcagagg aacttgatgc gttaacggcg
    20461 gattttattt cctataagcg tgacggtgtt ttgccagata tatttggtcg cgatgcactc
    20521 tacgacgact cgtttacctg gccattaatc aaatttgagc gagttgctca tattcatctg
    20581 gcaaatgtga ataatccatt tccgccacag ttgcgccaat tcagcagaac gaatgacgaa
    20641 gcgcatttgg tatattgtca gggtgcgttt gatgagcaag catggttgct cattgccatt
    20701 ctgaagcctg aacctcataa actggctcga gataacaacc aaatgcataa aattggaaaa
    20761 atggcagaag cgtttcgcat gcgtttttga atttatatta tgaataacat acaaataaga
    20821 gattatcagc ctggcgattt tcagcaacta tgcgctattt tcattagagc ggttacgatg
    20881 accgtcagtc agcattattc actacaacaa attgccgcct gggcgcagat tgacgaatct
    20941 cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagttattaa tgcaaaactg
    21001 gtcggcttta ttacctgcgt tgaacactat atcgatatgt tatttgttga ccctgaatac
    21061 acccgccgtg gggttgccag cgctttgtta aaacctttta ttaagtctga atccgaactt
    21121 acggtggacg caagcataac cgcaaaaccc ttttttgaac gttatggttt tcagacagtt
    21181 aagcagcagc gcgttgaatg ccggggagcg tggtttatta atttttatat gcgttataaa
    21241 ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg cctagtatgt gcgcgttttc
    21301 ttattccaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg actctttgca
    21361 gacctttcca ggattaattc tttttttctt gccctggatt cgtctgccat ttcctgattt
    21421 tttaattgtt actctcaata attcaagtcg cagcacatgc aacttgaagt atgacgagtc
    21481 tatttacgga atggtatatg ggcaattata ttcgtccctt atccgatgcg gtatttacca
    21541 tcgcatctga tgacctgtgg atcgagagtt tagcgatcca acaattacac accacggcaa
    21601 atttacccaa catgcagcgc gtagtgggga tgccagattt acaccccgga cgcggctacc
    21661 cgattggcgc agcgttcttc tccgcaggcc gtttttaccc agcactggtc ggcaatgata
    21721 tcggctgtgg catggcgcta tggcaaacag atattctcgc tcgcaaatac aacgccgata
    21781 agtttgaaaa gcgattatca gctttggatg acgttgctga agaaagctgg ctggaggaaa
    21841 acctgccgtc agcatttgca cagcatccgt ggcgcagctc gcttggctct atcggtggtg
    21901 gcaatcattt cgctgaactg caacaagttg atcaaatcat caacgctgaa ctgtttgcac
    21961 tggcaggtct ggatgcgcag catctgcaac tgctggttca tagcggctcg agggggctag
    22021 ggcagtctat tttacagcgg catattgcct cgttttcgca tcatggtttg cctgaaggca
    22081 gtgacgacgc gctggcgttt gcgcgtttta atcgccacct gatcgctttg cgcataatgc
    22141 aacaggttaa ggccaccgga agtccggttc tggatgtggc gcataacttt gttagcgcgt
    22201 gtcgaatcgg tgatcaacaa ggggtcttac atcgtaaagg tgccacgcct gatgactgtg
    22261 gtctggtggt tattcccggt tcacgcggtg attactccag gctggttcag cccgtcagga
    22321 gtgaggaaac attgcattcg ctggcgcatg gggctgggcg taaatggggg cgcaccgagt
    22381 gtaaagggcg tctggcagcg aaatacacag cgacgcaact ctcgcgtact gaacttggca
    22441 gtcgggtaat ttgtcgcgat aaacaactca tcttcgaaga agcgccacaa gcctataaat
    22501 cggctgaaag tgtggtgcaa tgtcagggca ggccggatta attattcctg tcgcgcggct
    22561 gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa ggatgatctt gctataactc
    22621 tcctcagcgc aggggccgga agaatgttgt ctcgcagtga aaaaggcgct ggacaggcta
    22681 attaaagaag ctgcccgaca agacgtcgcg gtaacggtgc tggaaacaga aacaggccgc
    22741 tactctgaca cgctgcgttc ggcgctgatt tctctggatg gcgacaacgc atgggcgtta
    22801 agcgaaagct ggtgtggcac tattcagtgg atttgcccga gcccgtatcg cccacatcac
    22861 gggcgtaaaa actggtttct gggcattggg cgttttaccg ctgatgagca ggagcaatcc
    22921 gatgcgatcc ggtatgagac gctgcgttcg tcggggccgg gcggtcaaca tgtcaataaa
    22981 accgactcgg cggtacgcgc cacgcatttg gcatccggta ttagcgtgaa ggttcagtca
    23041 gagcgcagcc agcatgctaa caaacggctg gcgcgattgc tgattgcctg gaagctggat
    23101 cagcagcaat aggaaaatag cgcggtgctg aaatcacagc ggcgaatgtt ccatcaccag
    23161 attgaacgtg gcaacccgcg acgaacgttt acagggatgg cttttatcga aggataatga
    23221 aggatgaaac tgccggaagg cgattaaacg ccatccggca gcaaatcaaa taattacttc
    23281 gccggaattt ctttcagcag ttcagtcagc agtgtccagt aatgacctac gctttcgatg
    23341 tgaacttgct catccggaga gtgtggaccg gtgatagttg gcccgataga aaccatgtcc
    23401 atttccggat acggtttttt gaacagacca cattccaggc ccgcgtggat aatctggatg
    23461 ttcggcgtct tgttgaacag gcgctgatag gtttcacgta ccagatgcat caccggagaa
    23521 ttagcgtccg gctgccagcc aggatatgcg cctttcgctt cggttttcgc gccagccagt
    23581 ttacccagcg aatccagcat gctcaccacg tagtctttac cgctgtcgat tagtgaacgg
    23641 atcaggcagt gaatttctac gttattgtca gtcatggtca ccacaccgac gttcagggag
    23701 gtttcgacca caccttttgc cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc
    23761 agcagacgaa taaaggtatc gcgagatttc gcaatcaggg cagctttatc gttcgctaca
    23821 gagtccagca acaaggccag atttttctct ttctctgcca gctcgttttt caggatctcc
    23881 tgataggtat tcaccagaga tttcagtgcg tcgactttat cagctgcgac agcaatggtc
    23941 gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc
    24001 aggtccagtt cttctgcatg acccgccagg aagcgcacca gcagtttgtt ggcattaccc
    24061 aggccaacgt ggatttcacc gccggagtga ccgcctttca gaccttttaa ggttaacttg
    24121 aaggtttcaa aaccagctgg aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg
    24181 ataccccccg cacaacccat gtagatttca ccttcttctt cggagtcggt gttaatcaga
    24241 atatcagcct gcaaccagtt gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg
    24301 gtcatggtca gcagcacttc cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc
    24361 agtgcagagg ccataccaat gccgttatcc gcacccagcg tggtgccgcg cgctttaacc
    24421 cattcgccat caatataagg ctggatagga tctttcgtga agtcatgcac ggtgtcgtta
    24481 tttttctgcg gcaccatatc gaggtgggcc tgtaagacga ccggtttacg attttccata
    24541 cctgcggtag caggttgacg aatcaggata ttacctacct gatcgcgttc gacatggaaa
    24601 cctttctctt ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg
    24661 tgaggaatag aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac
    24721 agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg
    24781 gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtattg agaataccac
    24841 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    24901 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    24961 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    25021 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    25081 aaggcattat tgccgtaagc cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac
    25141 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    25201 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    25261 tggtggatac cggtggtact gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    25321 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    25381 cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    25441 ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt tgttcttttt aacttcaggc
    25501 aggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    25561 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    25621 tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac catccctcac
    25681 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc
    25741 ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgacga tgatttatac
    25801 gataccgtga ttggctaccg tggtggcaac tggatttatg agtgggccac ccaggcgatg
    25861 gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac tcagtggtcg acactggctg
    25921 catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga tgacctggcc
    25981 gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc
    26041 acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat
    26101 atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct ggatgcgatg
    26161 cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg
    26221 actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca ggactccagc
    26281 ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc
    26341 gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc ataccttgaa
    26401 tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct gttgagtgat
    26461 tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag tcgtttgggg
    26521 atgcatgatg cttccgacga agcgttacgc gtggagctga atcgctattc attaaaagtg
    26581 caaggattgc tggggcgtcg ctgcccaacg ccaatgttat caggctactg gaagaacgat
    26641 ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga cggtaaatta
    26701 ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc
    26761 ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca
    26821 gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa gagcagattg
    26881 atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga agataatcga
    26941 ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa
    27001 ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta cagctaccag
    27061 tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga tactgaagtg
    27121 gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga actgctgacg
    27181 acgctgaatc tgaagctgga accggcgaat gattttcgtg atgaaccggt gaagttaacg
    27241 gcgtgagtga aatgtgccgg atgcatcaca tccggcaata ttcattaaaa ctgatacgtc
    27301 atcccaaccg cgacagtatc atcattatta atattcagct tattatcgct atccagttgg
    27361 ttgattttat aatcaacaaa cgctgacata tttttgttga aataatacgt agcaccgacg
    27421 tcgatataat tgaccagatc ttcatcaccg atgccctcaa tatctttccc tttcgataag
    27481 acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac cgcttcaaag
    27541 ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt ttcagaatag
    27601 aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca tgcttctgca
    27661 cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga gttggtatag
    27721 gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt gccgaagcca
    27781 tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg cagggttaag
    27841 ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag accgctggcg
    27901 cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc caccaaattc cgggaacata
    27961 tcggtccagg cttccacgtc atacaacgcg cccaggttac gaccataatc gaaagaaccc
    28021 aaatctttat atttcaaccc ggcaaaagcg agacgcgttt tttgctgtgc agtatcactc
    28081 tccgctttat ttccggcaaa ctccgcttcc caccgaccat aaccagtcag ttgatcgtta
    28141 atttgagttt cacctttaaa accaaaacgg atataactct ggtcgccatc tttactggcg
    28201 ttatcactca tataatgcat ggctttaact ttgccataga catccagttt attaccgtct
    28261 ttattatata tttctgcagc ctgtacagat gcagatgcca caatgcccat caccactaat
    28321 gccagagtgc tctttttcat tttcattcct gattttaatt aacgcgcgaa tattcagcgg
    28381 gagagccccg ttgaaaacag gaaagttttt aacctgagat tgttaaagat atattacaga
    28441 ttaatgatat tctgaaa
//
LOCUS       DAJTRW010000050        28405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRW010000050 DAJTRW010000000
VERSION     DAJTRW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO326_002308"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO326_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5966670.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO326_002309"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO326_002309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5966671.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO326_002310"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO326_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5966672.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO326_002311"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO326_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5966673.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO326_002312"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO326_002312"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5966674.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="OO326_002313"
     CDS             6427..7056
                     /locus_tag="OO326_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5966675.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO326_002314"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO326_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5966676.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO326_002315"
     CDS             complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO326_002315"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5966677.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="OO326_002316"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="OO326_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5966678.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="OO326_002317"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="OO326_002317"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5966679.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14056..14817
                     /gene="adiY"
                     /locus_tag="OO326_002318"
     CDS             14056..14817
                     /gene="adiY"
                     /locus_tag="OO326_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5966680.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14954..16291
                     /gene="adiC"
                     /locus_tag="OO326_002319"
     CDS             14954..16291
                     /gene="adiC"
                     /locus_tag="OO326_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5966681.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16395..18038
                     /gene="eptA"
                     /locus_tag="OO326_002320"
     CDS             16395..18038
                     /gene="eptA"
                     /locus_tag="OO326_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5966682.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18035..18703
                     /gene="basR"
                     /locus_tag="OO326_002321"
     CDS             18035..18703
                     /gene="basR"
                     /locus_tag="OO326_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5966683.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18704..19804
                     /gene="pmrB"
                     /locus_tag="OO326_002322"
     CDS             18704..19804
                     /gene="pmrB"
                     /locus_tag="OO326_002322"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5966684.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO326_002323"
     CDS             complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO326_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5966685.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO326_002324"
     CDS             complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO326_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5966686.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21747..22625)
                     /locus_tag="OO326_002325"
     CDS             complement(21747..22625)
                     /locus_tag="OO326_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5966687.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO326_002326"
     CDS             complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO326_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5966688.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="OO326_002327"
     CDS             25253..25588
                     /locus_tag="OO326_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5966689.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="OO326_002328"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="OO326_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5966690.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="OO326_002329"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="OO326_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5966691.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="OO326_002330"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="OO326_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5966692.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28405
                     /locus_tag="OO326_002331"
     CDS             28257..>28405
                     /locus_tag="OO326_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5966693.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7419 c         6935 g         6755 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaacaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ctggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ccagcttggc agccagtcgc cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgat gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgtaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttcccta gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac
    13861 cccacgcttt tttttgcctg ttatttatcc tgtaaaaaaa tatgtacatg agaaattact
    13921 ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata ttcatcatgc
    13981 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
    14041 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
    14101 aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc taaccatatg
    14161 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
    14221 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
    14281 caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg cctgacgcca
    14341 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
    14401 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
    14461 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta tcaaattatt
    14521 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
    14581 ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca aataatcacc
    14641 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
    14701 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
    14761 tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
    14821 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
    14881 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
    14941 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
    15001 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
    15061 ctccactggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
    15121 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
    15181 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
    15241 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
    15301 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
    15361 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgc gtgcaggccg ttgccactgt
    15421 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt tccgtggtga
    15481 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac
    15541 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
    15601 ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat tgattgccgc
    15661 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
    15721 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgctggggc
    15781 cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacgttgct
    15841 ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga tttttgcccg
    15901 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
    15961 cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg tttcttccgt
    16021 ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
    16081 ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca ttgccttcct
    16141 ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
    16201 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata aaaacccgta
    16261 tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa cataaaagcc
    16321 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
    16381 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
    16441 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
    16501 tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg tcgatgccgg
    16561 ttgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
    16621 gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc ataatgactt
    16681 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccagcagaaa
    16741 gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
    16801 agctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
    16861 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactgtttt
    16921 ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc ttaagcccct
    16981 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
    17041 tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa aaacgtaaaa
    17101 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
    17161 acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc cctaataccg
    17221 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgtg
    17281 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
    17341 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
    17401 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
    17461 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
    17521 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
    17581 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
    17641 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
    17701 ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
    17761 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
    17821 cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat caaaagcggt
    17881 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
    17941 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggccgcgg
    18001 atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg ttgaagacga
    18061 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
    18121 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca gcctggtggt
    18181 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
    18241 aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
    18301 cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg aagagttaca
    18361 cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
    18421 tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
    18481 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
    18541 acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
    18601 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
    18661 gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
    18721 tctgcgccga cctatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
    18781 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
    18841 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
    18901 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
    18961 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
    19021 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
    19081 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
    19141 aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
    19201 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
    19261 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
    19321 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
    19381 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
    19441 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
    19501 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
    19561 cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta aatgcgggga
    19621 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
    19681 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
    19741 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
    19801 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
    19861 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
    19921 gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat ccgtttcagc
    19981 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
    20041 aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
    20101 ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc
    20161 cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca tcaggtaata
    20221 ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
    20281 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
    20341 cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa ggatcaccgc
    20401 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
    20461 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
    20521 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
    20581 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
    20641 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
    20701 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
    20761 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
    20821 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
    20881 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
    20941 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
    21001 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccacc
    21061 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
    21121 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
    21181 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
    21241 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
    21301 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
    21361 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
    21421 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
    21481 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
    21541 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
    21601 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
    21661 atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
    21721 taggactcat tgatgtaact gattatttac gccaaattta cagagaagat ttcctcgacc
    21781 attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
    21841 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
    21901 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
    21961 tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg ttgtgtcgct
    22021 ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag acggcaggca
    22081 tcgccaaatg caccccagcg caactcctcc agaacgataa aacagcgccc aggcaacgac
    22141 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg atcacctgcc
    22201 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
    22261 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
    22321 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag atgaccggga
    22381 tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg cgcatttaac
    22441 atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg catcagtcgt
    22501 tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg cgccagatcc
    22561 actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
    22621 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
    22681 tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc agcctgacca
    22741 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattctgtca
    22801 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
    22861 gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg cttaatgctc
    22921 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
    22981 cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc gtgctctgct
    23041 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
    23101 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
    23161 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
    23221 attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
    23281 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
    23341 cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
    23401 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
    23461 gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa gcgacgttta
    23521 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
    23581 gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga agcaacgcct
    23641 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
    23701 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
    23761 ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac tcatagcgcg
    23821 cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt ggcgtaatac
    23881 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    23941 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    24001 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    24061 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    24121 cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa ctttccagcc
    24181 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    24241 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc aaacttttca
    24301 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    24361 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    24421 tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt ggcgcgacat
    24481 gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc gtcggcagcg
    24541 cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca ttaatggtgg
    24601 ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt cgggaaattt
    24661 tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga ctccagggta
    24721 aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc agtaactgca
    24781 actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca tacagggtct
    24841 gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt agccagattg
    24901 tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta tatttagtgt
    24961 gttgtttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca acctt
//
LOCUS       DAJTRW010000051        28399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRW010000051 DAJTRW010000000
VERSION     DAJTRW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            553..1323
                     /locus_tag="OO326_002332"
     CDS             553..1323
                     /locus_tag="OO326_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5966694.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1314..1787
                     /locus_tag="OO326_002333"
     CDS             1314..1787
                     /locus_tag="OO326_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5966695.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1894..2433
                     /gene="dnaT"
                     /locus_tag="OO326_002334"
     CDS             1894..2433
                     /gene="dnaT"
                     /locus_tag="OO326_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5966696.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2436..3173
                     /gene="dnaC"
                     /locus_tag="OO326_002335"
     CDS             2436..3173
                     /gene="dnaC"
                     /locus_tag="OO326_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5966697.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3222..3716
                     /locus_tag="OO326_002336"
     CDS             3222..3716
                     /locus_tag="OO326_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5966698.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3956..6247
                     /gene="opgB"
                     /locus_tag="OO326_002337"
     CDS             3956..6247
                     /gene="opgB"
                     /locus_tag="OO326_002337"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5966699.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6385..7407)
                     /gene="lgoD"
                     /locus_tag="OO326_002338"
                     /pseudo
     CDS             complement(6385..7407)
                     /gene="lgoD"
                     /locus_tag="OO326_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7546..8460
                     /locus_tag="OO326_002339"
     CDS             7546..8460
                     /locus_tag="OO326_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5966700.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8675..10036
                     /locus_tag="OO326_002340"
     CDS             8675..10036
                     /locus_tag="OO326_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966701.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10085..11749)
                     /gene="tsr"
                     /locus_tag="OO326_002341"
     CDS             complement(10085..11749)
                     /gene="tsr"
                     /locus_tag="OO326_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5966702.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11868..12314)
                     /gene="hpaR"
                     /locus_tag="OO326_002342"
     CDS             complement(11868..12314)
                     /gene="hpaR"
                     /locus_tag="OO326_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5966703.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12586..13875
                     /locus_tag="OO326_002343"
     CDS             12586..13875
                     /locus_tag="OO326_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5966704.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13872..15338
                     /gene="hpaE"
                     /locus_tag="OO326_002344"
     CDS             13872..15338
                     /gene="hpaE"
                     /locus_tag="OO326_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5966705.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15340..16191
                     /gene="hpaD"
                     /locus_tag="OO326_002345"
     CDS             15340..16191
                     /gene="hpaD"
                     /locus_tag="OO326_002345"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5966706.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16201..16581
                     /locus_tag="OO326_002346"
     CDS             16201..16581
                     /locus_tag="OO326_002346"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5966707.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16649..17452
                     /gene="hpaH"
                     /locus_tag="OO326_002347"
     CDS             16649..17452
                     /gene="hpaH"
                     /locus_tag="OO326_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5966708.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17463..18266
                     /gene="hpaI"
                     /locus_tag="OO326_002348"
     CDS             17463..18266
                     /gene="hpaI"
                     /locus_tag="OO326_002348"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5966709.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18288..19643
                     /gene="hpaX"
                     /locus_tag="OO326_002349"
     CDS             18288..19643
                     /gene="hpaX"
                     /locus_tag="OO326_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5966710.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19653..20543
                     /gene="hpaA"
                     /locus_tag="OO326_002350"
     CDS             19653..20543
                     /gene="hpaA"
                     /locus_tag="OO326_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5966711.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20570..21507
                     /locus_tag="OO326_002351"
                     /pseudo
     CDS             20570..21507
                     /locus_tag="OO326_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21734..23292
                     /gene="hpaB"
                     /locus_tag="OO326_002352"
                     /pseudo
     CDS             21734..23292
                     /gene="hpaB"
                     /locus_tag="OO326_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23310..23822
                     /locus_tag="OO326_002353"
     CDS             23310..23822
                     /locus_tag="OO326_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5966712.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24215..26365
                     /gene="btsT"
                     /locus_tag="OO326_002354"
     CDS             24215..26365
                     /gene="btsT"
                     /locus_tag="OO326_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5966713.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26415..26618
                     /locus_tag="OO326_002355"
     CDS             26415..26618
                     /locus_tag="OO326_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5966714.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26629..27585
                     /gene="yjiA"
                     /locus_tag="OO326_002356"
     CDS             26629..27585
                     /gene="yjiA"
                     /locus_tag="OO326_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5966715.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27654..>28399)
                     /locus_tag="OO326_002357"
     CDS             complement(27654..>28399)
                     /locus_tag="OO326_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5966716.1"
                     /translation="LENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNL
                     DDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNS
                     GFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNE
                     YWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKD
                     VCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6948 a         7425 c         7125 g         6901 t
ORIGIN      
        1 ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat
       61 actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct
      121 ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa tacttaatga
      181 aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc gcagaatgcg
      241 ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg ttaacactaa
      301 aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa attcattata
      361 gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga
      421 gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa
      481 ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac
      541 aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg tatccagtgt
      601 ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga
      661 ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg
      721 ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc
      781 attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg
      841 ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg ttacccaaga
      901 tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt
      961 ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta tatccgccag
     1021 ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc tttcgccgcc
     1081 accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc caccattgcg
     1141 atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc cgtcgccgat
     1201 atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca
     1261 ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg
     1321 tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg
     1381 gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct
     1441 cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt gagtggtcaa
     1501 cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc tggtatctgg
     1561 cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt
     1621 ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga
     1681 ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc
     1741 tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt
     1801 aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc
     1861 ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac
     1921 gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc
     1981 ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc
     2041 ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga tgtcgctctg
     2101 gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc
     2161 gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc gctatggggc
     2221 gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag
     2281 gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa
     2341 atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct
     2401 gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc
     2461 aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc
     2521 tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc
     2581 gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact
     2641 gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc
     2701 gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag
     2761 gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat
     2821 ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata
     2881 gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg
     2941 atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg
     3001 atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga
     3061 tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttatggg
     3121 tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat taatccccgt
     3181 ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa actattaaac
     3241 atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt
     3301 cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac
     3361 aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga aaccaagcct
     3421 taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg aagcaaaagc
     3481 tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga
     3541 acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt ttgctgaaaa
     3601 caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg
     3661 tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg
     3721 cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt
     3781 tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta
     3841 acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg
     3901 cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccattgtc
     3961 agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg
     4021 acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg tcgttttaaa
     4081 tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg cggttctcta
     4141 taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg
     4201 cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg
     4261 tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt
     4321 ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg
     4381 cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact
     4441 caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt
     4501 cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac
     4561 gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc agtgcggcat
     4621 accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct tcttcccgca
     4681 gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg
     4741 cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct tcgaccactt
     4801 atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca acgactgggg
     4861 attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc
     4921 aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat
     4981 ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc agtcattcag
     5041 cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca aagcgtcacc
     5101 gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc
     5161 gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa
     5221 gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct
     5281 ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc
     5341 gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc cagatatcat
     5401 ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat
     5461 gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg
     5521 cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt
     5581 cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac aactctgggc
     5641 tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg gcggtcagca
     5701 aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta aagatacggt
     5761 catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg ataacgatat
     5821 tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag aggtgaaaca
     5881 gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc agctgggcga
     5941 tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc
     6001 caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca
     6061 aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga
     6121 tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct
     6181 cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg tagaacgtga
     6241 agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc cttctccggc
     6301 ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg gacgctgcat
     6361 ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca actcacgatt
     6421 gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc gatgggttaa
     6481 catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag cgaaatcttc
     6541 cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt ccggatcgga
     6601 gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt gacgaatcag
     6661 attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt tctgcgccag
     6721 cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg ggtccagcac
     6781 gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat ccgccaccac
     6841 cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg gccccgcccc
     6901 gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat gagcgctaat
     6961 agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt
     7021 cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc caatgactga
     7081 aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct gacaggcaac
     7141 ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt tacccagccc
     7201 aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa atggttgatt
     7261 tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt ttattaatgc
     7321 ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt tcggctgctg
     7381 gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt gccttagata
     7441 tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa cacacgcata
     7501 aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag tcgttcacaa
     7561 aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg tcatatccct
     7621 tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg cactacagtg
     7681 cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg aaacgactat
     7741 gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc ttcaatgagt
     7801 gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg tcagttacgc
     7861 cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag cccggtggtt
     7921 gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga aaagcgcggc
     7981 cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga gctacgcgaa
     8041 atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga tccccgctgg
     8101 ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat cggcaatagc
     8161 tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc agctgccgac
     8221 aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt tcactatcaa
     8281 tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca tatgaccatt
     8341 ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg taaccatttg
     8401 aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta tgctcattaa
     8461 gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa gataaatacc
     8521 gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt tgtgagcggt
     8581 agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg cacaattacg
     8641 taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata tcaccctcga
     8701 tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac cagatggatt
     8761 agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt tattattttt
     8821 tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt taacgattcg
     8881 tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt tttcactcgc
     8941 ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc cacgcctgat
     9001 gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca tggtgcacag
     9061 ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc cgatgaaccc
     9121 atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac gcccgatggg
     9181 cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc tggcggcaat
     9241 gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg ggatttttct
     9301 cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga ctgccgttga
     9361 acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca attttgccga
     9421 atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat tcagtggcat
     9481 caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa cagcctataa
     9541 cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg gggctgccgg
     9601 gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg ctccgattaa
     9661 aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta cgctgatagt
     9721 accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac tgttctgtat
     9781 tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt ctcgcatgac
     9841 tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt tcgcgccgat
     9901 cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa tcatttgcgg
     9961 ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc cgatcaacga
    10021 cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg catttttatg
    10081 gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta cgcggcgtag
    10141 ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc tgttgctgaa
    10201 tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc gcggcggcgg
    10261 cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc atctcagcaa
    10321 ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa gctatttcgc
    10381 ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt tccccggcgc
    10441 tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc aggcttttaa
    10501 tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc gcaaccaccg
    10561 caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc aaagccagaa
    10621 tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt ttctgcgaac
    10681 tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg ccgcgttgcg
    10741 ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg gcgttctgtt
    10801 tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa gcggcctgtt
    10861 gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca ccgctataga
    10921 tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc tgcatatggc
    10981 gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc ggtttcacca
    11041 gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc gctaccagcg
    11101 aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc atcacgccca
    11161 ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg atatcataga
    11221 gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc tgatatccct
    11281 gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg atcagctccg
    11341 ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg gcggtgctct
    11401 gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc tgtttcagcg
    11461 aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata ttattctgat
    11521 ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac aacgcgaccc
    11581 agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg aaattttctt
    11641 tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa aggccaaaaa
    11701 cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg gtttctcttt
    11761 cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa aaggtgtaac
    11821 ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta ttcattatcg
    11881 cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa cagatgggtt
    11941 aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc ttcttcaatc
    12001 tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat atagagcttc
    12061 cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat ccgcgtgagg
    12121 atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg aaaatccatt
    12181 gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa attatgccgt
    12241 ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa cgcaatggtt
    12301 agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata gaaacaatta
    12361 atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca aaattgatga
    12421 tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa tagtttgtta
    12481 attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa taaaagtaat
    12541 cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa aggcactatc
    12601 ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc gttccagcaa
    12661 tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg caatacggtg
    12721 attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag cggtgcgact
    12781 gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc ggcagagtac
    12841 atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt ttaccgtccg
    12901 gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt ggctctcagc
    12961 aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga tcactggaat
    13021 accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga atttgccacg
    13081 ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga aatacagcca
    13141 agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac
    13201 gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca cggtacgctg
    13261 tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa gccaccggaa
    13321 gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca gacctccgtg
    13381 cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt tattggcaag
    13441 caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta caccgtgtgt
    13501 aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct gcgggtcaaa
    13561 agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc gatcccggac
    13621 ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca aggcaccacc
    13681 gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt tatgaccctg
    13741 aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt ggtgcctggc
    13801 gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag
    13861 gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg
    13921 actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg
    13981 gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg aaatgggctg
    14041 atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga
    14101 acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc catcagacca
    14161 aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa gtctgccagc
    14221 agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg ctggtgcaac
    14281 cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc gccacctgga
    14341 aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg gaactctccc
    14401 cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg gcgggcgtgc
    14461 taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt catcatgacg
    14521 tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg aaaaacgccg
    14581 ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt tttgaagatg
    14641 ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc aacggcgaac
    14701 gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa ttcgttaaac
    14761 gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg
    14821 ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc cgtctcggca
    14881 ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac ctgcctgcgc
    14941 acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat aaccgtatgc
    15001 gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag
    15061 ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac atctggacac
    15121 aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg gtgttcgtca
    15181 acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc tccggcaccg
    15241 ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac gtctgcattt
    15301 ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt agcgttagcc
    15361 gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa aaaccacggt
    15421 tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg ggaaatgggc
    15481 gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta tcacatcaac
    15541 tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt tattcgcgac
    15601 atgacctaca actacgaggg caacccggag ttggggcagc taattgccga ggaagcctta
    15661 aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga atacggcacg
    15721 ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc catttcggct
    15781 ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat tctgaaagcg
    15841 atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt
    15901 attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt cgaccgccag
    15961 atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt ttgcaatatg
    16021 ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac ggtgatgctg
    16081 ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac cgagctgttc
    16141 ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta aggacgtttt
    16201 atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg cttacctgag
    16261 ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct tgggggaata
    16321 cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca cgattacgcc
    16381 tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg tcaggaagtg
    16441 ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga gagccgttac
    16501 ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa acagaacaac
    16561 gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata aggcgctgcc
    16621 gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca ccctgatcgc
    16681 ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct cgctggatta
    16741 cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc gcctgaaaat
    16801 cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag cgatgcaggc
    16861 aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt tcttccacga
    16921 tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg agctggcttt
    16981 tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt acaacgccac
    17041 ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca tcgatccgga
    17101 aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca acgccggagt
    17161 gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga tctccgccct
    17221 gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc tgaatcatcc
    17281 ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac aactggaaac
    17341 cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta agggcgacac
    17401 cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt aaggagagaa
    17461 cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt ggattatggc
    17521 tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc gactggttat
    17581 tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa ctacaggcga
    17641 ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg gtgcaaatca
    17701 aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa aacgccgacg
    17761 aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc ggtgtgggca
    17821 gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa aaagccaacg
    17881 atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga
    17941 ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg agcgccgata
    18001 tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag caggcgatcg
    18061 tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag caactggcaa
    18121 aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg
    18181 cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt tcgatggcga
    18241 agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg acgacctcta
    18301 ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt aataaattat
    18361 ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt gatcgcatca
    18421 atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc acaatgtttg
    18481 gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc agtaatatga
    18541 tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc tggggaattg
    18601 cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg cgtatgctgg
    18661 ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc tggtggttcc
    18721 cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca gtcactatga
    18781 tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg aatctaaaag
    18841 gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt gtcgtcacct
    18901 ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac gaaaaacagg
    18961 ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca tctgttccac
    19021 gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt gcttattttt
    19081 gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta cagagtttca
    19141 atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa ttctgcacaa
    19201 tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc aaaaagcata
    19261 cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg acagatcaca
    19321 gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc gcgatgacta
    19381 tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt gcgatggctg
    19441 tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc ggtattttga
    19501 aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg ctggtactgg
    19561 gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt tcatctgaac
    19621 ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc tttgaacatg
    19681 gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt gaagatgtgc
    19741 attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc cctcactggc
    19801 acgatagata ttttcaactc cattatctgg tcactggacg cattacttta caactggatg
    19861 agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg gtgccacata
    19921 ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag gagttaattt
    19981 ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta caaggtatct
    20041 gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat tggccgataa
    20101 tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa tcactggcgc
    20161 aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca acatgcagcg
    20221 tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa aattatcgcc
    20281 aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca cggcttaccg
    20341 agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa cgagtgagcc
    20401 gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata gcccttgatc
    20461 ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg ggttgctcac
    20521 caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca tgaaaatatc
    20581 tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct acgcctccga
    20641 accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt tattaaaagc
    20701 tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt taattgatgc
    20761 tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc agattgataa
    20821 tattttactc acccatttac acagtgatca tattctggac tatccatcag tcttaatgaa
    20881 tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc caggcacccg
    20941 agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc gtatggaaga
    21001 agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg aaggcgtagt
    21061 ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc ctttccctga
    21121 cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct tctcaggaga
    21181 tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac ttgtgcatga
    21241 ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg gtaaaacact
    21301 ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa ttgccaatga
    21361 agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc cggatgaaac
    21421 atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca tgaaaatatg
    21481 acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct tgttagcggg
    21541 gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta tttaactcca
    21601 ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca accgcgttca
    21661 acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc cgattaatac
    21721 tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc ctttcaccgg
    21781 ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt
    21841 gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg cccaactgta
    21901 cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg acaccggcag
    21961 cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca
    22021 acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc gtaccccaga
    22081 ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg gtcagttcga
    22141 gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact ttaaccacgc
    22201 gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt ttacatcaag
    22261 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
    22321 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
    22381 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa attaatctcc
    22441 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
    22501 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat cccatgggaa
    22561 aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga aggcggtttc
    22621 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga cttcattacg
    22681 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtgcaggcc
    22741 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
    22801 tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
    22861 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga acgcaacgtt
    22921 accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
    22981 cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca gcgcatcaag
    23041 atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca cgagctgtat
    23101 gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg ccaggcacaa
    23161 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
    23221 cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat gctggataag
    23281 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc ctgcgctttc
    23341 atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    23401 gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca ccgtcgctga
    23461 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggcaagttgt
    23521 gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg ggcatgacag
    23581 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    23641 cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg caggcgattg
    23701 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    23761 gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa gctgcgattt
    23821 aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    23881 atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc gcttacatcc
    23941 ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg gcaacatcac
    24001 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    24061 tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag tgagtgaatc
    24121 cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg cacctgtaag
    24181 ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    24241 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    24301 tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    24361 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    24421 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    24481 gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg gaccggttct
    24541 cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtgctggc
    24601 cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    24661 cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc tgtttggctg
    24721 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    24781 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    24841 tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta ttggtatcgt
    24901 gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt actggggtcc
    24961 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    25021 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    25081 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat
    25141 gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    25201 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    25261 cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    25321 aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca tcatcgaacc
    25381 gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca tgcctaacct
    25441 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    25501 acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    25561 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg cgccgacgct
    25621 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    25681 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    25741 ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    25801 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    25861 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    25921 cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    25981 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    26041 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    26101 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    26161 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    26221 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt
    26281 gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc cggaaggcgg
    26341 cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg gctttgttct
    26401 tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg
    26461 cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg aagaccaacc
    26521 atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag aacgcgcgct
    26581 acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat gaacccgatt
    26641 gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct gcgccatatt
    26701 cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg cgaagtctcc
    26761 gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac caacggctgc
    26821 atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct ggataatctc
    26881 gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat ggccgatccc
    26941 ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta cctgctggac
    27001 ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca gttcaccatc
    27061 gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga cgtcgcaggc
    27121 gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc ggtctacacc
    27181 gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt tatgctggaa
    27241 gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca aaacgatatt
    27301 tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc ccgcgtgatg
    27361 gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat gctgtggatt
    27421 gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag cgccgactgg
    27481 gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg gattcagttg
    27541 ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg ggaaagatca
    27601 actttttgag agttccaccg caacattatt acgagtggaa ctctttagat atgtcagacc
    27661 aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag ttcagccaat
    27721 ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt actattaagt
    27781 ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga atttttggaa
    27841 ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc ttgaggttgg
    27901 aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc aattgtgatt
    27961 ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa gcgaagcaaa
    28021 attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc atccagagca
    28081 gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga atcaaaaggc
    28141 atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg gcttttaatt
    28201 tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa ttgagaaaaa
    28261 tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat aaccggagga
    28321 atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat tgaagggtgt
    28381 tcaaaatcat tctctaaaa
//
LOCUS       DAJTRW010000052        27601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRW010000052 DAJTRW010000000
VERSION     DAJTRW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO326_002358"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO326_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5966717.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO326_002359"
     CDS             complement(1614..1937)
                     /locus_tag="OO326_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5966718.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO326_002360"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO326_002360"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5966719.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO326_002361"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO326_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5966720.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO326_002362"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO326_002362"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5966721.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO326_002363"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO326_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5966722.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO326_002364"
     CDS             7537..8574
                     /locus_tag="OO326_002364"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5966723.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO326_002365"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO326_002365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5966724.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO326_002366"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO326_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5966725.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO326_002367"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO326_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5966726.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO326_002368"
     CDS             10745..11626
                     /locus_tag="OO326_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5966727.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO326_002369"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO326_002369"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5966728.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO326_002370"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO326_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5966729.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO326_002371"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO326_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO326_002372"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO326_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5966730.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO326_002373"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO326_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5966731.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO326_002374"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO326_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5966732.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO326_002375"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO326_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5966733.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO326_002376"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO326_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5966734.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO326_002377"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO326_002377"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5966735.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO326_002378"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO326_002378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5966736.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO326_002379"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO326_002379"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5966737.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO326_002380"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO326_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5966738.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO326_002381"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO326_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5966739.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO326_002382"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO326_002382"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5966740.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO326_002383"
     CDS             24893..25273
                     /locus_tag="OO326_002383"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5966741.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO326_002384"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO326_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5966742.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO326_002385"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO326_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5966743.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="OO326_002386"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="OO326_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6865 c         7236 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatactaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAJTRW010000053        26514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRW010000053 DAJTRW010000000
VERSION     DAJTRW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            346..639
                     /locus_tag="OO326_002387"
     CDS             346..639
                     /locus_tag="OO326_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5966744.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            711..1370
                     /locus_tag="OO326_002388"
     CDS             711..1370
                     /locus_tag="OO326_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5966745.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1462..1908
                     /locus_tag="OO326_002389"
     CDS             1462..1908
                     /locus_tag="OO326_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5966746.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OO326_002390"
                     /pseudo
     CDS             complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OO326_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3388..3807
                     /locus_tag="OO326_002391"
     CDS             3388..3807
                     /locus_tag="OO326_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5966747.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3807..5075
                     /gene="umuC"
                     /locus_tag="OO326_002392"
     CDS             3807..5075
                     /gene="umuC"
                     /locus_tag="OO326_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5966748.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO326_002393"
     CDS             complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO326_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5966749.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO326_002394"
     CDS             complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO326_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5966750.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7559..8278
                     /gene="fadR"
                     /locus_tag="OO326_002395"
     CDS             7559..8278
                     /gene="fadR"
                     /locus_tag="OO326_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5966751.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8330..9862)
                     /locus_tag="OO326_002396"
     CDS             complement(8330..9862)
                     /locus_tag="OO326_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5966752.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10192..11490
                     /gene="dadA"
                     /locus_tag="OO326_002397"
     CDS             10192..11490
                     /gene="dadA"
                     /locus_tag="OO326_002397"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5966753.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11500..12570
                     /gene="dadX"
                     /locus_tag="OO326_002398"
     CDS             11500..12570
                     /gene="dadX"
                     /locus_tag="OO326_002398"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5966754.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12738..14152)
                     /locus_tag="OO326_002399"
                     /pseudo
     CDS             complement(12738..14152)
                     /locus_tag="OO326_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14763..16499)
                     /locus_tag="OO326_002400"
     CDS             complement(14763..16499)
                     /locus_tag="OO326_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5966755.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="OO326_002401"
     CDS             complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="OO326_002401"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5966756.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17608..18219
                     /gene="emtA"
                     /locus_tag="OO326_002402"
     CDS             17608..18219
                     /gene="emtA"
                     /locus_tag="OO326_002402"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5966757.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASISGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18221..18955)
                     /locus_tag="OO326_002403"
     CDS             complement(18221..18955)
                     /locus_tag="OO326_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5966758.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19156..19410
                     /locus_tag="OO326_002404"
     CDS             19156..19410
                     /locus_tag="OO326_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5966759.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19460..20920)
                     /locus_tag="OO326_002405"
     CDS             complement(19460..20920)
                     /locus_tag="OO326_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5966760.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20857..21414)
                     /locus_tag="OO326_002406"
     CDS             complement(20857..21414)
                     /locus_tag="OO326_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5966761.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21440..22294)
                     /locus_tag="OO326_002407"
     CDS             complement(21440..22294)
                     /locus_tag="OO326_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5966762.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22294..23103)
                     /locus_tag="OO326_002408"
     CDS             complement(22294..23103)
                     /locus_tag="OO326_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5966763.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23113..23871)
                     /locus_tag="OO326_002409"
     CDS             complement(23113..23871)
                     /locus_tag="OO326_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5966764.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23868..24848)
                     /locus_tag="OO326_002410"
     CDS             complement(23868..24848)
                     /locus_tag="OO326_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5966765.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24848..25870)
                     /locus_tag="OO326_002411"
     CDS             complement(24848..25870)
                     /locus_tag="OO326_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5966766.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6658 a         6463 c         6843 g         6550 t
ORIGIN      
        1 tggcctttgc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
       61 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc
      121 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
      181 tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg
      241 ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat
      301 tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt
      361 tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg
      421 cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg
      481 ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc
      541 gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt
      601 tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt
      661 atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac
      721 atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca
      781 gtaactatgc caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt
      841 ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt
      901 tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg
      961 caacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat
     1021 tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt
     1081 ttgataactc ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac
     1141 aaaatacaac gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg
     1201 acatgatcca taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg
     1261 ccggtgacgt tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg
     1321 agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc
     1381 cgccagaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt
     1441 aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac
     1501 gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat
     1561 aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc
     1621 aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc
     1681 attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat
     1741 cggttgctgg cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa
     1801 gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg
     1861 attgactggc aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag
     1921 atgctgatgg gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt
     1981 tgaaagcgct ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca
     2041 atctttaaaa aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag
     2101 agaataatcg cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc
     2161 tgatactcat tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt
     2221 aaatcatcat aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg
     2281 gcggctattt cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta
     2341 accgtgttag acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct
     2401 ggaatcagtt tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat
     2461 ggaccggcga cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc
     2521 tgtgactgga aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc
     2581 agcagtttcc cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct
     2641 tcattcagct tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc
     2701 attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca
     2761 ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt
     2821 aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc
     2881 ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag
     2941 atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc
     3001 aacgatttca gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt
     3061 tttacaacta ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc
     3121 aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga
     3181 atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt
     3241 cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga
     3301 caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa
     3361 aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
     3421 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca
     3481 gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact
     3541 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
     3601 ctgattgtcg atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
     3661 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
     3721 agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg
     3781 atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg
     3841 ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga
     3901 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg
     3961 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca
     4021 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
     4081 cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta
     4141 attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc
     4201 atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg
     4261 cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc
     4321 agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca
     4381 gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc
     4441 ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg
     4501 aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct
     4561 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     4621 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     4681 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac
     4741 tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg
     4801 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     4861 gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc
     4921 aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg
     4981 ccgggcaggg gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata
     5041 caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc
     5101 ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc
     5161 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     5221 tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     5281 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     5341 caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     5401 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg
     5461 gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata
     5521 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     5581 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     5641 aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     5701 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     5761 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     5821 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag
     5881 tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     5941 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     6001 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     6061 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     6121 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     6181 atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     6241 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     6301 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     6361 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     6421 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     6481 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     6541 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     6601 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     6661 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     6721 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg
     6781 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     6841 atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc
     6901 atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt
     6961 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata
     7021 gatacccgcc accataaaca tcaataacag caagacttca agatttgccg ccacctcttc
     7081 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc
     7141 ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     7201 caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat
     7261 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     7321 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat
     7381 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     7441 aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta
     7501 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     7561 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     7621 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     7681 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     7741 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa
     7801 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     7861 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     7921 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     7981 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     8041 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     8101 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     8161 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     8221 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     8281 ccttccgttt aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     8341 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     8401 gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat
     8461 ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat
     8521 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     8581 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     8641 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     8701 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     8761 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     8821 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     8881 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     8941 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     9001 aggtcgcaca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     9061 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     9121 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg
     9181 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     9241 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     9301 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     9361 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     9421 cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt
     9481 tatgtccata gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca
     9541 tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac
     9601 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     9661 taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa
     9721 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     9781 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     9841 tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     9901 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     9961 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
    10021 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
    10081 tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
    10141 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
    10201 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
    10261 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
    10321 gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa
    10381 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
    10441 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
    10501 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
    10561 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
    10621 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
    10681 tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg
    10741 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt
    10801 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
    10861 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg
    10921 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
    10981 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
    11041 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
    11101 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
    11161 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
    11221 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
    11281 gttgtcggga gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
    11341 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
    11401 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
    11461 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
    11521 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
    11581 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
    11641 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
    11701 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
    11761 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    11821 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    11881 atgaatcggt tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca
    11941 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat
    12001 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt
    12061 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    12121 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    12181 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    12241 ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    12301 gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta
    12361 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat
    12421 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    12481 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg
    12541 gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc
    12601 ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca
    12661 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    12721 ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact
    12781 tcagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg
    14101 aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac
    14161 gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag
    14221 gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca
    14281 gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta
    14341 aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata
    14401 agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt
    14461 cagtgcggca ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg
    14521 gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg
    14581 ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc
    14641 cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt
    14701 tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa
    14761 atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct
    14821 tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt
    14881 gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct
    14941 cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga
    15001 atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta
    15061 ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt
    15121 cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc
    15181 tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat
    15241 tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc
    15301 ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt
    15361 ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca
    15421 ttctccagcc ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat
    15481 cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca
    15541 gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga
    15601 atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg
    15661 atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc
    15721 ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc
    15781 gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg
    15841 gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagaccgatg
    15901 acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt
    15961 tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat tggatcatta
    16021 ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc
    16081 agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa taagccttca
    16141 atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt
    16201 acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga actggcctga
    16261 gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac catgtaggcg
    16321 aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag catgccgatc
    16381 gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata
    16441 ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaat
    16501 ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga
    16561 cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct
    16621 aatcgtcaac tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag
    16681 cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg
    16741 ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt
    16801 gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata
    16861 gagctgcaac agcatacgtt cgacgcggaa agggtgcacg ttaatatctt ccagcaccag
    16921 aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag
    16981 attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat
    17041 ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc
    17101 atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc
    17161 cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg
    17221 ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta
    17281 accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag
    17341 atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc
    17401 gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg
    17461 ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg caattccttg
    17521 ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg
    17581 cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg
    17641 ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt
    17701 ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc
    17761 gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg
    17821 gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaatctc cggacgtgat
    17881 gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag
    17941 cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc
    18001 attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt
    18061 gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg
    18121 gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc
    18181 tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg
    18241 ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg
    18301 tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc
    18361 catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga aaaacacccc
    18421 attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat tcggcaggct
    18481 ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag cggaaacgta
    18541 acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga
    18601 tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtaataaatg
    18661 ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac
    18721 cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg
    18781 cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt ttgctgatca
    18841 gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca
    18901 cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga
    18961 aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa aacttaatac
    19021 aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc ccagcatcga
    19081 tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc gattcatcga
    19141 aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt
    19201 atcggcaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc
    19261 gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tgacggctcc
    19321 atcagcggtt ttaatttgca cagcttcctg gtggcggtgg tgggcgctat cctggtgctg
    19381 ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat
    19441 gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt
    19501 cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat
    19561 tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg
    19621 cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg
    19681 ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa
    19741 gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca
    19801 atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat
    19861 ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg
    19921 catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac
    19981 cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg
    20041 ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg
    20101 tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca
    20161 cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct
    20221 cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga
    20281 cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc
    20341 gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg
    20401 caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc
    20461 tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt
    20521 acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt
    20581 cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc
    20641 agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct
    20701 gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa
    20761 tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat
    20821 tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca
    20881 aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac
    20941 tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc
    21001 tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc
    21061 ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg ggacataaat
    21121 gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg
    21181 ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg
    21241 agcgacattc tgtttatcca aggccttcat ggtgggttga tcgagaacgg tggtcgtcgt
    21301 cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg
    21361 ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata
    21421 aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag
    21481 ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat
    21541 ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg
    21601 acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta
    21661 cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa
    21721 ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt
    21781 catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat
    21841 gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg
    21901 cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt
    21961 gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc
    22021 cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt
    22081 gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg
    22141 cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct
    22201 ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta
    22261 acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc
    22321 ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg
    22381 ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac
    22441 gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa
    22501 atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg
    22561 taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct
    22621 gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg
    22681 gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc
    22741 actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc
    22801 ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt
    22861 gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt
    22921 ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga
    22981 ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt
    23041 gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag
    23101 catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac
    23161 ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac
    23221 tcgtccatct ggctctactt ggataatgct gtcggcaatg gcgttggcat ggagcgtatg
    23281 gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag
    23341 ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg
    23401 ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt
    23461 ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag
    23521 gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc ccatcaacac
    23581 catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc
    23641 ctgtgcgcgc tggcgatggc tgagtgtctg cacagagagg ctatccagta ggatctcgcc
    23701 ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt
    23761 cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac
    23821 tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct
    23881 ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt
    23941 gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg
    24001 ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca
    24061 atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac cgtgctggcg
    24121 gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc
    24181 gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg
    24241 ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg
    24301 gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg atcggccagc
    24361 gttttaatta gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc
    24421 agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc
    24481 accatcagcc cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg
    24541 attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact
    24601 aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt
    24661 aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca
    24721 gactcatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc
    24781 ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg
    24841 attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga
    24901 acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc
    24961 aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc
    25021 caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc
    25081 agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg
    25141 gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt
    25201 gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc
    25261 ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg
    25321 tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc
    25381 ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt
    25441 ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga
    25501 tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc
    25561 cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa
    25621 tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca
    25681 ttttttctgg cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg ctgacgatgc
    25741 gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa
    25801 aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg
    25861 ttatgggcat ggtgaacatc ctgaaaagta aaatcgatat atactttcat atatatcgat
    25921 aaaaatcatg ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg
    25981 caagcctgtt gaccctctct atcattaaaa atgtatttat caggtggcta atattattta
    26041 aatcatttaa ggctaaatta tttaaatatg aatgacatta ttgttttttt atcgtccacg
    26101 gttacgcgac gttgaagtta cttgttttat aatgagcgat ttttgatatc tcgccgagaa
    26161 aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag gtgaaaagaa
    26221 tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt gctgatagta
    26281 agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac gacgttatta
    26341 atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta ctgaatgcgt
    26401 ttattcggac gtgcaaaaaa agagatgata acagttacgg aaatatgata tttacgcttt
    26461 attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac ggcg
//
LOCUS       DAJTRW010000054        24935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRW010000054 DAJTRW010000000
VERSION     DAJTRW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..634)
                     /gene="yeaG"
                     /locus_tag="OO326_002412"
     CDS             complement(<1..634)
                     /gene="yeaG"
                     /locus_tag="OO326_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5966767.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAK"
     gene            1070..1816
                     /gene="mipA"
                     /locus_tag="OO326_002413"
     CDS             1070..1816
                     /gene="mipA"
                     /locus_tag="OO326_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5966768.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            1906..2667
                     /locus_tag="OO326_002414"
     CDS             1906..2667
                     /locus_tag="OO326_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5966769.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(2718..3602)
                     /locus_tag="OO326_002415"
     CDS             complement(2718..3602)
                     /locus_tag="OO326_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5966770.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3686..4681)
                     /gene="gapA"
                     /locus_tag="OO326_002416"
     CDS             complement(3686..4681)
                     /gene="gapA"
                     /locus_tag="OO326_002416"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5966771.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5023..5436
                     /gene="msrB"
                     /locus_tag="OO326_002417"
     CDS             5023..5436
                     /gene="msrB"
                     /locus_tag="OO326_002417"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5966772.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5478..5750
                     /locus_tag="OO326_002418"
     CDS             5478..5750
                     /locus_tag="OO326_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5966773.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6120..7196
                     /locus_tag="OO326_002419"
     CDS             6120..7196
                     /locus_tag="OO326_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5966774.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7223..8602
                     /locus_tag="OO326_002420"
     CDS             7223..8602
                     /locus_tag="OO326_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966775.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            8619..9664
                     /locus_tag="OO326_002421"
                     /pseudo
     CDS             8619..9664
                     /locus_tag="OO326_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            9685..10521
                     /locus_tag="OO326_002422"
     CDS             9685..10521
                     /locus_tag="OO326_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5966776.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10526..11473
                     /locus_tag="OO326_002423"
     CDS             10526..11473
                     /locus_tag="OO326_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5966777.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11483..12463
                     /gene="ydjG"
                     /locus_tag="OO326_002424"
     CDS             11483..12463
                     /gene="ydjG"
                     /locus_tag="OO326_002424"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5966778.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12600..13358
                     /locus_tag="OO326_002425"
     CDS             12600..13358
                     /locus_tag="OO326_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5966779.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13476..14834
                     /locus_tag="OO326_002426"
     CDS             13476..14834
                     /locus_tag="OO326_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966780.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(14927..15568)
                     /gene="pncA"
                     /locus_tag="OO326_002427"
     CDS             complement(14927..15568)
                     /gene="pncA"
                     /locus_tag="OO326_002427"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5966781.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15579..16595)
                     /gene="ansA"
                     /locus_tag="OO326_002428"
     CDS             complement(15579..16595)
                     /gene="ansA"
                     /locus_tag="OO326_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5966782.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(16762..18618)
                     /gene="sppA"
                     /locus_tag="OO326_002429"
     CDS             complement(16762..18618)
                     /gene="sppA"
                     /locus_tag="OO326_002429"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5966783.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18779..19330
                     /locus_tag="OO326_002430"
     CDS             18779..19330
                     /locus_tag="OO326_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5966784.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19447..20490
                     /gene="selD"
                     /locus_tag="OO326_002431"
     CDS             19447..20490
                     /gene="selD"
                     /locus_tag="OO326_002431"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5966785.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20495..22456
                     /gene="topB"
                     /locus_tag="OO326_002432"
     CDS             20495..22456
                     /gene="topB"
                     /locus_tag="OO326_002432"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5966786.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22583..23623
                     /locus_tag="OO326_002433"
     CDS             22583..23623
                     /locus_tag="OO326_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5966787.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23740..>24935)
                     /gene="gdhA"
                     /locus_tag="OO326_002434"
     CDS             complement(23740..>24935)
                     /gene="gdhA"
                     /locus_tag="OO326_002434"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5966788.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6351 a         5794 c         6433 g         6357 t
ORIGIN      
        1 ttttggcgat agcaatttgt tgcagaattg acggccagac cttcactacc cggaacttag
       61 tgatatcgcc accaaattca tgcaggcgtt ttgccgccca tggcgacatg atggtgccga
      121 gataacggcg aggaatgcca tactcttttt ccagaatctg agcatcttcc tgcggattga
      181 aaagacagaa cggatgatcg ttgaccgggc tacgctcacc gttggcgctc aatacataaa
      241 tcggtacgag ctgcattaat gatttcagtc gctcagcaag cgatgattta cccccaccca
      301 caggccccag cagatacagg atttgtttct tctcttccag cccctgagcc gcgtgtttca
      361 gataagagac aatctgttca atcgcgtctt ccatgccgta aaactcttca aatgccggat
      421 aacgtgcaat gacccggtta gaaaagagtc gagaaagtct ggggtcctgg gctgtatcga
      481 ccatgacagg ctcaccgata gccatcaata gccgctcagc cgcgttggca taagcactgc
      541 gatcttgccg acaagtggta agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt
      601 catatcgctg gcgatagtga tcgaatatat tcatggtatg ccgtcctttc gttttttagc
      661 acaggttaag agccgttcat atgaatagta gaggctcccg gaagaggtaa actgaacgac
      721 gtctcttgct aaaacacagc aactctcatg ccattttatc gacgatgaga acaacgtgcc
      781 cggtgataca ccttcaaaat taagcgtaga tggcaaatgg aaaacttgcc tgcacggctt
      841 tcttaatttc aatggcatat caacaactca tccataaatt ttgcataatt aatgtaaaga
      901 ccaggctcgc cagtaacgct aaatccattt ggctgtaagc gcggtgtcat ccgcggcagg
      961 aaaattaaac agttacttta aaaaatgaaa acgtaaaaag gttgggtttc gatgtattga
     1021 cgggtaaact ttgtcgcccg ctaaacattt gtttaaggaa tgattaattg tgaccaaact
     1081 caaacttctg gcacttggag tgcttatcgc aacgtctgca ggcgtagcgc acgctgaagg
     1141 taaattttcc ctgggcgcag gcgtaggtgt cgttgagcac ccatataaag attacgatac
     1201 cgatgtttac ccagtaccgg taatcaacta tgaaggcgat aacttctggt tccgtggctt
     1261 aggtggtggt tactacctgt ggaatgacgc aacggataaa ctttcaatta ccgcttactg
     1321 gtcgccgctt tacttcaaag cgaaagacag tggcgatcac caaatgcgtc acctggatga
     1381 ccgtaagagc accatgatgg ctggtctgtc ttatgctcac tttacccagt acggttacct
     1441 gcgtaccacc ctggctggcg ataccctgga taacagcaac ggcattgtct gggatatggc
     1501 ctggttgtat cgttacacca acggtggcct gaccgtgact ccgggtattg gtgtgcagtg
     1561 gaacagcgaa aaccagaacg aatactatta tggcgtatcg cgcaaagagt ccgctcgcag
     1621 cggtctgcgt ggctataacc cgaatgacag ctggagccct taccttgagc tgagcgccag
     1681 ctacaacttc ctcggcgact ggagtgttta cggtaccgcg cgctacaccc gtctgtctga
     1741 tgaagttact gacagcccga tggtggataa atcctggact ggcctgattt ctaccgggat
     1801 cacctacaaa ttctgataat gcatctttcc agggcatttt gtgcataaaa acagggcgtc
     1861 acgccctgtt ttgcattacg gtggtgcaat acctgggagg aaacaatgca acaaaaaatg
     1921 attcaattta gtggcgatgt ctcactgcca gccatagggc agggaacatg gtatatgggc
     1981 gaagatgcca gtcagcgcaa aacagaagtt gctgcactac gcgcaggcat tgaactcggt
     2041 ttaaccctca ttgataccgc cgaaatgtat gccgatggcg gtgccgaaaa ggtggttggg
     2101 gaagcattaa ccggtctgcg agagaaggtc tttctcgtct ctaaagtcta tccgtggaat
     2161 gctggcgggc aaaaagcgat aaatgcatgc gaagccagtt tacgccgtct caatactgac
     2221 tatctcgatc tctacttatt acactggtct ggcagtttcg cttttgaaga gactgttgct
     2281 gcgatggaaa aattgatcgc ccagggaaaa atccgtcgct ggggcgtttc taaccttggt
     2341 tatgccgata tgcaggaact ctggcagctg ccggggggaa atcagtgtgc cactaatcag
     2401 gtgctttacc atctcggttc acgagggatt gagtacgatc tactcccctg gtgccagcaa
     2461 cagcagatgc cggtgatggc ttacagtcca ttagcccagg ccgggcggtt gcgcaatgga
     2521 ctgttaaaaa acgcggtagt caacgaaatt gcacatgtcc aacaacatgc agctgcgctt
     2581 gaggtcgaac tttcgtcagc ggaattagct atgctggata aggcatatcc ggcaccaaaa
     2641 ggaaaaactg cgctggatat ggtgtgatgt tgatacgtgc cggatgcatc gcacccggca
     2701 cacaacatta acgcaaatta acgtttggca acgcgaatgg attgcgccag atgtgcaggt
     2761 ttctctttgg tgactttttg cgtttctgaa gcgtaagccg tttctacaca aacaaaggtc
     2821 ttgtagccat cgtccggcat gtcgcccatg ctaactgaaa gcgccggtcc cgggttccag
     2881 ccgacaacgt tcagatgatg ctggtggcct acggcgataa tacgattcag cgcttcatca
     2941 ttaatcacgc tgcaatcttg cggattcaga tacacgcgat cggtacggtc agggaaggtc
     3001 tgaataccgt cggtcagtac atcttctttc gcgtcattca ctttatcaat gaagcgatcg
     3061 cccagcccac tgacgcttac cttagcgata tcacccacgt taaagtaggt atgcagggca
     3121 gaggtggttt caaattcgcc atgtgattca agatcgattt cacaggtttt acccacgcgg
     3181 aaatgcgcta acagcgtaaa gtcgtgcggc cagaattttt tcgtctcttc gctttgtgtc
     3241 aattcaaaag tcagcgctac gccatcagca tcttcatgat gtgatttcag cgtccacggc
     3301 aggttgcggg caaaaccgtg cgcaggcaga ccttgttgtg ccgccggacc aaaccacggc
     3361 cagcaaaccg gtacgccacc gcgaatagcg acgccatttt tgaacggtgt gttgttgctc
     3421 aaccacagaa cttcttcttc acccgcaggt ttccacgaga gaaggtgtgc gccctgtaat
     3481 gcaaaagagg cttttacctg gggatgatcg accacaatga ggtccagttc atccagttta
     3541 cgacgggaga ggacagggga gatttgttcg atgaccggaa gggcaaaaat tttcttaatc
     3601 atgacgcagt cctttaactt cattttatca ggtaaaaaaa agagcgaccg aagtcgctct
     3661 ttttagatca cagtgtcatc tcaacttatt tggagatgtg agcgatcagg tccagaactt
     3721 tgttggagta accggtttcg ttgtcgtacc aggataccag tttcacgaag ttgtcgttca
     3781 gagcgatacc agctttagca tcgaacacgg aagtgcaaac ttcgccgttg aaatcggtag
     3841 atactacgtc atcttcggtg tagcccagaa cgcctttcat ttcgccttca gcagcagctt
     3901 taacggcagc tttgatctgc tcgtaagttg cagctttttc cagacgaacg gtcaggtcaa
     3961 ctacggatac gttcggggtc ggaacgcgga acgccatacc agtcagtttg ccattcagtt
     4021 ctggcagtac tttacctaca gctttagcag caccggtaga ggacgggatg atgttctggg
     4081 aagcgccgcg gccgccgcgc cagtctttgt gagacgggcc atcaacggtt ttctgagtag
     4141 cggtagtagc gtgaacggtg gtcatcagac cttcgatgat gccgaagtta tcgttgataa
     4201 ctttagccag cggagccagg cagttggtgg tgcaggaagc gttggaaacg atgtcctggc
     4261 cagcatattt gtcgaagtta gcgcctttaa cgaacatcgg agtgttgtct ttagacggac
     4321 cagtcataac cactttcttc gcaccagcgg tgatgtgttt acgagcagtt tcgtcagtca
     4381 ggaacagacc agttgcttca gcgacaacgt caacacccac ttcgtcccat ttcaggttag
     4441 ccggatcacg ttcagcggta acacggattt ttttaccgtt aacgatcaga tgaccgtctt
     4501 tcacttcaac ggtaccgtcg aaacggccgt gagtggagtc atatttcagc atgtatgcca
     4561 tgtaatcagc gtctaacagg tcgttgattg caacgatctc gatgtcagaa cgtttctgag
     4621 cagcacggaa aacaatgcga ccgatacggc caaaaccgtt gatacctact ttgatagtca
     4681 tatattccac cagctatttg ttagtgaata aaaggttgcc tgtaaaatta caaaaacctt
     4741 acgcagcgtc aagcggaatc gtgtcaatca ttgcgacaaa tcaatcctgt gcctaagcat
     4801 tacgcgactg actcgcctca cttttccttt gggcttgaga ccacatgggg tcggcgcccc
     4861 gaattttaaa gggcaattac gataaaaatg tgattttcat cacgatttag gtgcagccat
     4921 ttcgtcgaga tcaagtttag gacaaaattt tgccagttgg tgttaatgtt ttgttagaat
     4981 cagtcacgta atgtgagcac gattaaagtg agatgtgagt aaatggcgaa taaaccttcg
     5041 gcagaagaac tgaaaaaaaa tttgtccgag atgcagtttt acgtgacgca gaatcatggg
     5101 acagaaccgc catttacggg tcgtttactg cataacaagc gtgacggcgt atatcactgt
     5161 ttgatctgcg atgccccgct gtttcattcc gaaaccaagt atgattccgg ctgtggctgg
     5221 cccagtttct acgaaccggt gagtgaagaa tccattcgtt atatcaaaga cttgtcacat
     5281 ggaatgcagc gcatagaaat tcgttgcggt aactgtgatg cccatctggg gcatgtcttt
     5341 cccgacgggc cgcaaccaac aggcgaacgt tattgtgtta actctgcttc tttacgcttc
     5401 actgatggcg aaaacggcga agaaatcaac ggttgaaaaa acgattcagc aacttattcc
     5461 acaggagcgg aaaaattatg aatcttgatg acattatcaa cagcatgacg cctgaggtat
     5521 accagcgttt gtcgaccgcc gttgaactgg ggaaatggcc tgatggcgtt gcgttgaccg
     5581 aggaacaaaa agagaattgc ctgcaactgg tgatgctgtg gcaagcccgc cataataccg
     5641 aagcacagca tatgacaatt gacacaaatg gtcagatggt catgaagagc aaacagcagt
     5701 taaaagaaga ttttggtatc tcagcaaagc ctattgccat gtttaagtaa gtggttcatc
     5761 ccctttttgc cgcggctatg aaggggatag ccaacaatga ccccacgact cgtctcaatt
     5821 atctcgaccc gacctggcta agtcccagtc cgatctcttg cagtcgcgtt cacgcctgaa
     5881 tgacttctcc tgaacttaag tctcagacct atttggccgg taatccctct cgaatttgct
     5941 ctttttgcgt cccgccttcc accaacctgt aacagaccca gccattttct gcgtaattgt
     6001 gatttgagtt gtaaaatgca ggtcattatc tgtgttgtgc agatttaata ttgacctcaa
     6061 aaatcatgtg ggtaagtatg atggccatct aagcaaaaca aaacacagag gatgacgcaa
     6121 tgaaagcact ggctcggttt ggcaaggcct ttggcggcta caagatgatt catgtcccac
     6181 aacccatttg tggcccggaa gacgtagtga ctgaaattaa agccgcggca atctgcggcg
     6241 cagacatgaa gcactacaat gtcgatagcg gttctgatga gtttaactct atccgcggcc
     6301 atgagttcgc aggttgtatt gcgcaggttg gtgaaaaagt caaagactgg aaagtggggc
     6361 aacgcgtcgt gtcggataac agcggtcacg tttgcggcgt ttgtccggcc tgtgaacagg
     6421 gtgattttct gtgttgtaca gaaaaagtaa accttggtct ggataacaat acctggggcg
     6481 gtggtttttc caaatattgt ctggttcctg gtgaaattct caaaattcat cgtcatgcgt
     6541 tgtgggaaat ccctgatggt gttgattatg aggacgcagc cgtacttgac cctatctgta
     6601 atgcctataa atccatcgcg cagcaatcga aattccttcc tggtcaggat gttgtcgtca
     6661 ttggcactgg cccactcggg ctgttttccg tacaaatggc gcggattatg ggggcggtaa
     6721 atatcgtcgt cgttggtctg caagaagatg tggcggtccg cttcccggtt gcaaaagaac
     6781 tgggggcgac ggcagtagta aatggttcta ccgaagatgt ggtggcgcgc tgccagcaaa
     6841 tttgtggcaa agataatctg ggactggtga ttgaatgctc cggtgccaat atcgcactga
     6901 aacaagccat cgaaatgctc cgcccgaatg gggaagtggt acgcgttgga atgggcttca
     6961 aacctcttga tttctcgatt aatgacatta ccgcctggaa taaaagcatc attgggcata
     7021 tggcctatga ctccacctca tggcgtaacg ctatccggct attagccagc ggcgctatca
     7081 aagtcaaacc gatgatcacg catcgtatcg gcctgtcgca atggcgcgaa gggtttgatg
     7141 cgatggtcga taaaaccgca atcaaagtga tcatgactta cgactttgat gaataactac
     7201 tctcctgaag gaacgttatc gcatggaaca gataacaaaa ccgcattgtg gtgcacggct
     7261 ggatcgctta ccggattgcc gctggcattc gtcaatgttt gccatcgtcg cgtttggctt
     7321 gcttgtctgc tggagtaatg ccgttggtgg cttgatcctc gcgcagctga aagcgttggg
     7381 ctggacagat aattccacta cagccacatt ctcagcaatc acgaccgccg gaatgtttct
     7441 cggtgcgctg gtgggcggca tcattggcga caaaaccggt cgcagaaatg cgttcatcct
     7501 ctatgaggcc attcatattg cctcgatggt tgtcggtgct ttctcaccga atatggattt
     7561 tcttattgcc tgccgttttg tgatggacgt tgggctggga gctttactgg ttacactgtt
     7621 tgctggtttc accgaatata tgcccggtag aaatcgcgga acgtggtcca gtcgggtttc
     7681 ctttattggc aactggtcat atccgctctg ctcactgatt gcgatgggac tcacgccgct
     7741 gattagtgca gagtggaact ggcgggtaca actgcttatt cctgcaatat tgtcgcttat
     7801 cgctacggcg ctggcctggc gctactttcc tgaatccccg cgctggctgg aatcgcgcgg
     7861 acggtatcag gaagccgaga aagtcatgcg gagtatagaa gaaggcgtca tacgccagac
     7921 gggtaaacct ttgccgcctg tggttattgc tgatgacggt aaagcgccac aagcggtgcc
     7981 gtattcagcc ttactgacag gagtattact gaaacgcgtg atattaggct cttgtgtgct
     8041 gattaccatg aacgttgtgc agtacacact aattaactgg ttgccaacaa tattcatgac
     8101 ccaggggatt aatttaaaag actcgattgt tttaaatacc atgagtatgt ttggcgcgcc
     8161 atttggtatt tttattgcca tgctggtgat ggataaaatt ccgcgtaaaa caatgggtgt
     8221 ggggctatta atcctgattg cggtgctcgg atatatctat tcactgcaaa ccagtatgtt
     8281 gctcataacg ctgattggtt tcttcctgat tactttcgtc tatatgtacg tttgctatgc
     8341 ctcggcagtg tatgtccctg aaatctggcc gacagaggcc aaactccgtg gctccggtct
     8401 ggcgaatgcg gtagggcgaa tcagtggtat tgccgcacct tatgccgttg cagtgctgct
     8461 cagtagttat ggtgtaacgg gagtctttat tcttctgggg gctgtttcaa atattgtcgc
     8521 aattgctatc gccaccattg gaattgaaac caaaggtgtc tccgttgaaa gtttaagtat
     8581 tgatgcagta gccaataaat aatacgggtg gattcaaaat gaaaaattca aaagcaatat
     8641 tgcaggtgcc gggcacaatg aaaattattt cagcagaaat accagtgcct aaagaagatg
     8701 aagttttgat taaagtagaa tatgtcggta tttgtggttc agatgtacat ggttttgaat
     8761 caggcccgtt tattccgcct aaagacccaa atcaagaaat tggcctgggt catgaatgcg
     8821 ccgggacggt tgtggctgtg ggaagccgcg tgcgcaaatt taaaccgggg gatcgggtaa
     8881 atatcgaacc tggcgttcct tgcggtcact gtcgttactg tctggaaggc aaatataaca
     8941 tctgcccgga cgttgatttt atggcgacac aacccaacta ccgcggcgca ttaacgcact
     9001 atctgtgtca tccggagagc tttacttaca aactgcccga caatatggac acgatggaag
     9061 gggcgctggt ggagcctgcc gcagtcggga tgcatgccgc gatgctggca gatgttaaac
     9121 cgggtaagaa gataattatt ctgggagcag gttgtattgg tttgatgacg ttgcaagcgt
     9181 gcaaatgcct gggcgcaacg gaaattgccg tcgttgatgt actggaaaaa cgtctggcaa
     9241 taggggaaca gcctggtgcg acagtggtta ttaatggcgc aaaagaggac actattgcac
     9301 gctgtcagca atttaccgaa gacatgggcg cagatattgt tttcgaaaca gcgggttctg
     9361 cggtcaccgt taaacaggca cctttaatct ggtaatgcgc ggcggtaaaa ttatgattgt
     9421 tggtactgta cccggcgatt cggcaatcaa tttcctcaaa atcaatcgcg aagtcactat
     9481 ccagacggta ttccgctatg ccaatcgtta tccggtcacg attgaagcta tttcttcagg
     9541 gcgattcgat gtgaaatcga tggtgacgca tatttacgat tatcgggatg tacaacaggc
     9601 atttgaagag tcagttaaca acaaacgcga cattattaaa ggcgttatta aaattagcga
     9661 ttaattaata cgggagaaca gaacatgctc gcagatatca gatattggga aaacgatgcc
     9721 acaaataaat attatgcaat tgcccatttt aacgtatgga atgcagaaat gttgatgggc
     9781 gttaaagacg cagccgaaga agcgaaatcc ccggttatta tttcttttag tacaggtttt
     9841 gtcggtaaca cctcatttga agatttctct cacatgatgg tatcaatggc acaaaaagca
     9901 acggtgccgg taataactca ttgggatcat ggtcggagta ttgagattat tcataacgcc
     9961 tggactcatg gtatgaattc attaatgcgt gatgcttctg catttgattt cgaagaaaat
    10021 attcgtttaa ccaaagaggc tgttgatttc tttcatccgc tgggtattcc ggtagaggcg
    10081 gaattagggc atgtcggtaa tgaaaccgtt tatgaggaag cgctggcggg ctatcactat
    10141 accgatcctg accaggcggc tgaatttgtt gaacgtacgg gctgtgactc attggctgtc
    10201 gccatcggca accagcatgg ggtttatacg tcagagccgc aattgaactt tgaggtcgtc
    10261 aaacgcgtac gcgatgctgt ttctgttccg ctggttttgc atggcgcatc ggggatcagt
    10321 gatgccgaca ttaaaactgc aatttcgttg ggtattgcga aaatcaacat tcatacggag
    10381 ctctgtcagg ccgcaatggt agcagtgaaa gaaaaccagg atcagccttt cctgcattta
    10441 gaacgtgaag tacgtaaagc tgtaaaagaa cgggcattgg aaaaaattga actgttcggt
    10501 tctgatggca aagcggaata atgatatgga taatctcgac gttatttgta taggtgccgc
    10561 tattgttgat attccattac aaccggtcag taaaaatatc tttgatgtag attcttatcc
    10621 tcttgaaaga atcgcaatga gcaccggggg cgatgcaata aatgaagcaa caattatttc
    10681 tcgtctgggg catcgtaccg cattaatgag ccgcattggt aaagatgcct cagggcaatt
    10741 tattctcgac cattgtcgca aagagaatat tgatattcaa agcctgaaac aggacgtcag
    10801 catagataca tctattaacg ttggactggt gacggaggat ggcgagcgga cgtttgtcac
    10861 gaaccgtaac ggcagtttgt ggaaattaaa tattgacgat gttgattttg cgcggtttcc
    10921 tcaggcgaaa ttattatcac tggccagtat tttcaacagt cctctattgg atggtaaagc
    10981 gctaacagaa atttttacgc aagcgaaagc ccggcagatg atcatttgtg ccgatatgat
    11041 caaaccgcgg ttgaatgaaa cgctggatga tatttgcgaa gcattgagtt atgtcgatta
    11101 tctgtttcca aattttgccg aggaaaaatt actcacaggg aaagagacac tggatgaaat
    11161 tgctgactgc tttcttgcgt gcggcgtaaa aacggtggtg attaaaacgg gtaaagacgg
    11221 ctgctttatc aagcgtggtg acatgacgat gaaggtgccg gcggtcgcag gaataaccgc
    11281 catcgacacc attggcgcgg gcgataactt tgcttcaggt tttattgcgg cactgttaga
    11341 aggcaaaaat ctgcgtgaat gcgcacgctt tgccaatgca acggcggcta tctcggttct
    11401 aagcgtcggt gccaccaccg gcgtaaaaaa cagaaagctg gtcgaacaat tgctggaaga
    11461 atacgaagga taatgaaggc aaatgaaaaa gataccttta ggcacgacgg atataacgct
    11521 ttcgcgaatg gggttgggga catgggccat tggcggcggt cctgcatgga atggcgatct
    11581 cgatcggcaa atatgtattg atacgattct tgaagcccat cgttgtggca ttaatctgat
    11641 tgatactgcg ctaggatata actttggcaa tagtgaagtt atcgtcggtc aggcgttaaa
    11701 aaaactgccc cgtgaacagg ttgtagtaga aaccaaatgc ggcattgtct gggaacgaaa
    11761 aggaagttta ttcaacaaag ttggcgatcg gcagttgtat aaaaaccttt ccccggaatc
    11821 tatccgcgaa gaggtagaag ccagcttgca acgtctgggt attgattaca tcgatatcta
    11881 tatgacgcac tggcagtcgg tgccgccatt ttttacgccg atagctgaaa ctgtcgcagt
    11941 gcttaatgag ttaaaggctg aagggaaaat tcgcgctata ggcgctgcta acgtcgatgc
    12001 tgaccatatc cgcgagtatc tgcaatatgg tgaactggat attattcagg cgaaatacag
    12061 tattctcgac cgggcaatgg aaaacgaact gctgccgcta tgtcgtgata atggcattgt
    12121 ggttcaggtt tattccccgc tagagcaggg attgttgacc ggcactatca ctcgtgatta
    12181 cgttccgggc ggcgctcggg caaataaagt ctggttccag cgtgaaaaca tgctgaaagt
    12241 gattgatatg cttgaacagt ggcagccact ttgtgctcgt tatcagtgca caattcccac
    12301 tctggcactg gcgtggatat taaaacagag tgatttaatc tccattctta gtggggctac
    12361 tgcaccggaa caggtacgcg aaaatgtcgc ggcactgaat atcaacttat cggatgcaga
    12421 cgcaacattg atgagggaaa tggcagaggc cctggagcgt taaatactca acacgattca
    12481 cctctgtttc tgtgaaaact gtgatgaaaa tctgtgattc aaataggtta ttttgaaagt
    12541 aaacatcggt tatgcgataa tcgcgctaat gtgatgtaaa aagatacagg ggtgtaatcg
    12601 tggcggcaaa agacaggatt caggcaatta agcaaatggt tgccaacgat aaaaaggtga
    12661 cagtctcaaa tttgagtggg atttttcagg taaccgaaga aaccattcgc cgcgatcttg
    12721 agaagctgga agatgaaggc tttttgacca gaacctatgg tggtgctgtt ttaaatacag
    12781 cggtgttgac ggagaatatc catttttata agcgcgcttc atcgttttat gaagagaagc
    12841 tgcttattgc acgcaaggca ctacccttta tcgacaataa aaccaccatg gcagccgatt
    12901 ccagtagtac cgtaatggaa ttgctcaaat tgttacagga ccgtagtgac ctgacgttgc
    12961 taaccaactc ggcggaagcg attcatgttc tggctcagtc agaaattaaa gtcgtttcaa
    13021 cgggtgggga actaaacaaa aatacacttt ccctgcaagg aagaattact aaagagatca
    13081 tcagccgcta ccatgtcgat atcatggtaa tgagttgtaa aggtcttgat attaacagcg
    13141 gcgcgctgga ctctaatgaa gcggaagctg aaatcaaaaa gacaatgatc cgtcaggcga
    13201 cagaagttgc gttattggtt gatcactcta agtttgatcg taaagctttt gtccagttag
    13261 ctgactttag tcatattaat tatataataa ctgataaatc accgggtgca gagtggatag
    13321 cattttgcaa agacaataat atccagctgg tgtggtaatt taagcacacg ttgtcaatat
    13381 tatttgttcc tgcgactgga aataaaatga tgataaagat gcggcaagtg aatttgccgc
    13441 aattgctacc ttttatagca aatccgaggc acccaatgga acaatatgat caaattggcg
    13501 caagactgga ccgcttgcct ttggcccggt ttcattatcg tatatttggt ataataagct
    13561 ttagtctgtt attaacgggg tttttgagtt actcaggtaa tgtcgtctta gcaaagctgg
    13621 taagcaatgg atggtcaaat aatttcctct atgccgcctt tacctcggca ttaatgtttg
    13681 gttatttcat cggctcactt actggtgggt ttattggtga ctactttggg cggcgcaggg
    13741 cgtttcgcat aaatcttctc atcgtcggta ttgctgcaac aggggccgct tttgtccctg
    13801 atatgtactg gctcattttc tttcgcttcc tgatgggaac aggaatgggg gcgctgatta
    13861 tggttggcta tgcctcattt acggagttta tccccgcgac ggtgcgtgga aaatggtccg
    13921 cgcggctctc atttgttggt aactggtcgc ccatgctgtc tgcggcgata ggcgtggtgg
    13981 ttatcgcttt ttttagttgg cgaataatgt ttcttctggg tggtattggc atactgttag
    14041 cctggtttct ctcaggtaaa tactttatcg agtcgccacg atggctggaa gggaaagggc
    14101 aaatcgccgg tgcagaaagc caacttcgtg aagtagagca gcaaattgaa agggagaaga
    14161 gtattcgttt accccagctt actttgaacc agagcaacag caaggttaag gtaatcaagg
    14221 gtaccttcag gctcctgttt aaaggggaaa tgttacgacg tacattagtt gcgataactg
    14281 ttttaattgc aatgaacatt tcgctttata ccattaccgt atggataccg accatatttg
    14341 ttaactccgg cattgatgtc gataaatcaa tattaatgac cgctgttatt atgattggtg
    14401 ctccggtagg aatatttatt gcggcattaa ttattgatca ttttcctcgt cgattatttg
    14461 gctccgcctt acttattatt attgccgtgt taggctatat ctattcaagt cagactacag
    14521 agtgggcgat tttaatctat ggtctggtga tgatcttctt tttatacatg tatgtttgct
    14581 tcgcgtcggc ggtttatatc ccggagcttt ggccaacgca tttacgcctg cgcggttcgg
    14641 gtttcgttaa tgccgtcgga cggatcgtcg cagttttcac gccctatggc gttgcggcat
    14701 tattaacaca ttatgggtcg atcacggtgt ttatggtgct tggtgtcatg ttagtgctct
    14761 gtgcgctggt tctctccatt tttggcatcg aaacgcggaa ggtgtcgctg gaagagattt
    14821 ctgaggtgaa ttaaatcttt cgttgatggc atcaattgcc agatgtacca ctgacgtgtt
    14881 ttatttggcc tacaatgcca gtacagaatt gtaggccagc gtaaaattac ccctgcgtct
    14941 cttcccagtc tgccagcgta tatagcgttg ccccagctgc tgacatctcc ataaacgcgt
    15001 gtgcactgtc ctggggctgg atattcacgc cacgacagcc atcggtaatc acgtttacct
    15061 tataacctaa ctgtaacgcg tccagcacgg taaacttcac gcaatagtca gtagccaggc
    15121 ccataacgat caattcatcg atttcatgat cgcgcaacca gtcatcaagc gcggttttct
    15181 gccgacgtcc gttatcaaaa aaggcactgt aactgtcaac taaaggattt tcgcctttat
    15241 ggaacaccgc tgcgatctct ttttgattca gtaacggatg taattgtgcg ccttcactgt
    15301 tctgcacaca gtgttcaggc cagaaagttt gccgcaggcc gccgagttgg cctggcgtat
    15361 aaggctctac gccgtgctgg ctggcaaagc tgccgtgatt cgccggatgc cagtcctggc
    15421 tggcgataac cgcttctccg cgcgactggc accagtcaat aaggcggtta gcaacctcca
    15481 ccgtactgtc accttccggt acggcgagcg cgccaccagc acagaaatca ttttgtaaat
    15541 cgaccagtaa cagggcgcga tggggcattg cagtctcctt aatcatccgg cgtcagttcg
    15601 ccgcgcaggt tttggctcat ggccttgcga atggtttcag tatccagttc ctggctcagc
    15661 aggtaatgca gtttggttag cgtggcttct acagtcatat ctgcaccgtc aataacgccg
    15721 gcatgggcga gggcgttacc ggtggcataa ccactcatat tcactttacc ggacatacat
    15781 tgtgttaggt tgaccaccac aataccgcga tcgctggctt cttgtaattc ctgcaggaag
    15841 gctttgtttt gtggcgcatt acccacgcca taggagcgca gaatcaatgc tttcaccggt
    15901 tggcgcagaa aattgcgcac gacgtcagca gaaatccctg gataaatcgt caccacgcca
    15961 attggttgtg gcgtgattgg atgaacgatt aattcccctt caccgtgcgg ggcgggtggc
    16021 gtattcaaac gacgaatatg gataccagct tccagtaacg gaggaaggtt tggagaggca
    16081 aacgcatcaa aaccatcggc atgggctttg gtagtgcggt tgccgcgata caatcggtta
    16141 ttgaaaaaga gcgttacttc gttgatcgga taattcggcg caacgtacaa cgcattcagc
    16201 agattaattt gtccgtcaga gcgtaactca gccagcggga tttgtgaccc tgtcacaatg
    16261 accggtttac cgagattctc gagcatgaac gacagcgcag aggcggtata cgccatcgtg
    16321 tcggtaccgt gtagaatgac aaaaccatca tagtcgtcat agtgcgcttt aatatcttca
    16381 gcaatatgct gccagtcttc cggcgtcata tcagaagaat ccatcaacgg cgtatattca
    16441 tgaatggtga aatctggcat ctccgggcga tggaattccg gcatcagcgc cagttggcgt
    16501 tgtagatgac ctgacaccgg aatataaccc tgctcggaac gctgcatccc gatggtcccg
    16561 cccgtgtagg cgacgtaaat tgatttcttt tgcatgatat tgatatgaat gatatcgaaa
    16621 gagcaaaagt atatacgtga ggcaggagga gcgcacctga tactatgcgg ataaggaaat
    16681 atggggcgag acagcgggaa gaggattcgt cgaggtaaaa aataatgtgg ccgacgatct
    16741 gtcggccact cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa acgcataacg
    16801 gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg tagaggctac
    16861 cgaggcaagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg cccggacaga
    16921 accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag
    16981 atgccactgt ttcacttttg cgagctctgc tgctttggca accgcatcat cgaaatcccc
    17041 gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt ggccctgggc
    17101 gattttatca atttgctccg gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg
    17161 tttatagcca ttctcaatgc ttaattgcat catctgctgc gcttccggcg gcagtgccct
    17221 ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa caccaatcga
    17281 atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac cggtcagggt
    17341 gctggggtta gccacaatgt aattagctgg cgtggaaatc cagtaaccac cagatgccgc
    17401 catgccgccc atcgatacaa ccacagactt acccgctgcc cgggctgctg ccagttcagc
    17461 gcgaatcact tcagacgcgg taacgctgcc gcctgggcta ttaacacgca ggacaatcgc
    17521 tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac
    17581 attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga caccgatgct
    17641 gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt
    17701 tttatcagtc ttactccagc cgaactcttt ggtcagagct ttttcgattt cggcactcga
    17761 tgccagtgca tcgaccagct tgttttccag tgcatatttc gcggtatcgc caccggtttt
    17821 ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag ggatctgccg
    17881 gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc ggctgtcagc
    17941 ttcgcgggct gccggtgaca tatcatcacg aataaacggt tcaacggcag atttatacgt
    18001 acccacgcgg aacacatggg tggaaacttt cagcttatcc agcaacgatt tgtagtacag
    18061 accgttggta gcaaaaccgt gcagatcaac cacgccttgc ggtgacagcc aaattttatt
    18121 ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg cataaaccgg
    18181 tttcccgctg tcacgaaact ctttcagggc tttgccgatg tactgcatag acggctggtc
    18241 gccgcctgcg aagtttttca gatccatcac gataccggtg atattgcggt cgtccttcgc
    18301 ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgcagacgat cggaactggc
    18361 accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtctggtt tatcgacgat
    18421 cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg aatcaccacc
    18481 actgacctgc atccagatcc ccacaccaac cagtacgagg aaaataaaga acaggttaag
    18541 taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa
    18601 tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtcac aggcgcaata
    18661 gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg actgtgctgt
    18721 aacaaaatcc actctcgtgt taattttgtg aataaatatc acgacaggag ttaatcaaat
    18781 ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc
    18841 aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg accataagtc
    18901 catgcaaccg tggcattttt ttgtgattga aggggaaggg tgcgagcgtt tcagcgccgt
    18961 actggaacag ggggcgattg ctgccggtag tgatgataaa gctatcgaca aagcccgtaa
    19021 tgcgccgttc cgcgcaccgc tcatcatcac ggtggtggcg aaatgcaaag aaaatcataa
    19081 agtcccgcga tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc
    19141 agccgttgcc cagggatttg gcggcatctg gcgcagtggc gcattaactg aaagtccggt
    19201 agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc tctacctcgg
    19261 cacgccgcag ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac
    19321 ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca ggaattcaga
    19381 cactctcact tatcacttca cggaatgagg gttaccatac ccggattgca ttgacaggag
    19441 atgtccatga gcgagaactc gattcgtttg acccaataca gccacggagc tggttgcggc
    19501 tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt
    19561 gatccgaatt tgcttgtggg taatgaaacc cgcgacgatg cggcggtgta cgatctgggc
    19621 aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga taatcctttc
    19681 gattttggcc gcattgcggc gactaacgcc atcagcgata tcttcgcgat ggggggcaaa
    19741 ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc ggaaattgcc
    19801 cgcgaagtaa ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc gctggctggc
    19861 ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg gatcgtaccg
    19921 accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt cctgacgaaa
    19981 ccgctgggga tcggcgttct taccacggct gagaaaaaat cactgttgaa accagaacat
    20041 cagggactgg cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac
    20101 atcgaaggcg taaaagcgat gaccgacgtt acgggctttg gtctgctggg ccacttgagc
    20161 gaaatgtgtc agggggctgg tgtgcaggca cgcgtcgact atgaagcgat cccgaaactc
    20221 cccggtgttg aagagtacat taagttgggc gcagtacctg gcggcactga acgtaacttt
    20281 gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgatct gctgtgcgat
    20341 ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa tgaggttaaa
    20401 actacagccg ccgagtttgg cattgaactg acggcgattg gcgaactggt gccagcgcgc
    20461 ggcggtcgtg ccatggttga gatccgttaa tttaatgcgg ttgtttattg ccgaaaaacc
    20521 gagtctggcg cgcgccattg ctgatgtcct gcccaaaccg caccggaaag gcgatggctt
    20581 tatcgagtgc ggtaatggtc aggtggtgac ttggtgtatc ggtcacctgc ttgagcaggc
    20641 gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt tgccgattgt
    20701 cccggaaaag tggcaattac agccacgacc ctccgtgacc aaacaactta acgtcatcaa
    20761 acgcttcctg catgaagcca gcgaaatcgt tcacgccggg gacccggatc gtgaagggca
    20821 attgctggtg gatgaagtgc tggactatct gcaactggca ccggaaaagc gccagcaggt
    20881 gcagcgttgc ttgataaacg acctgaaccc gcaggcggtt gagcgggcga tcgaccgtct
    20941 tcgttccaac agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga
    21001 ctggctgtac ggcatcaata tgacccgtgc gtataccatt ctcggtcgca atgccggtta
    21061 tcagggcgta ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg
    21121 cgatgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt
    21181 gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta
    21241 ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg ttaaccgcat
    21301 tagtggtcaa ccggctattg tcaccagcta taacgataaa cgggaatcag aatccgcgcc
    21361 gctgccgttt tcgctttcgg cgttgcagat tgaagcggca aaacgcttcg gtctgagcgc
    21421 gcagaacgtg cttgatatct gccagaagct gtacgaaacg cacaagctaa tcacttatcc
    21481 gcgttccgat tgtcgctatt tgccagaaga acattttgcc ggacgccacg cggtgatgaa
    21541 tgccatcagc gttcatgcac cggatctgtt gccgcagcca gtggtagatc cagatatacg
    21601 caaccgctgt tgggatgaca aaaaggtcga tgcgcaccac gccatcatcc cgaccgcacg
    21661 gagttctgcg atcaacctga cggagaacga agcgaaggtc tataacctga ttgcccgtca
    21721 atacctgatg cagttctgcc cggatgcggt gttccgcaag tgtgttatcg aactggacat
    21781 tgccaaaggc aaatttgtcg ctaaagcccg ttttcttgct gaagcaggct ggcgcacgct
    21841 gttaggcagc aaagagcgcg atgaagaaaa cgacggcacg ccattgcctg tggtggcgaa
    21901 aggcgatgag ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc agccgccgcg
    21961 ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga
    22021 caaagatctg aaaaaggtcc ttcgtgcgac cgatggtctg gggacagaag caacccgtgc
    22081 cgggattatt gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca
    22141 ctccaccgac gccggaaaag cgttattcca ttcgctgcca gaaatggcga cgcgaccgga
    22201 catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca
    22261 ggactttatg cagccgctgg tggggacgct atatcagctt attgatcaag ccaaacgtac
    22321 gccggtgcgg cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg ctgataagaa
    22381 aaaggctgca ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg aagccggaag
    22441 cggggcgata gcgtaagcga gcgaatcttt cgtgctattc gagtcatatt ctgaaatatc
    22501 cagcggatca agaaagttcg ttggatattt ttttgcatgg ataaaattat cgcctctaaa
    22561 gtatgtaata acagggaatg tgatgaaaaa agttttattg caaaaccatc ctgggagcga
    22621 gaagtattct tttaatggct ggggaatatt taatagtaat tttgaacggg tgattaaaga
    22681 aaataaggcc atgctgcttt gtaagtgggg gttttattta acatgtgttg tcgctgtaat
    22741 gtttgtattc gcagcgataa catccaacgg tttgaatgaa agaggcctga ttaccgcggg
    22801 atgctctttt ctttatctat taattatgat ggggcttatt gttcgggccg gttttaaagc
    22861 aaaaaaagaa caactgcatt attatcaggc taaaggtatt gagccgctca gtatcgagaa
    22921 gttacaggcg ctacaattga tcgcacctta tcgattctat cataagcaat ggtctgaaac
    22981 gctagagttc tggccgcgaa agcctgaacc tggcaaagat accttccaat atcatgtgct
    23041 tccttttgac tcgatcgata tcatcagtaa aagacgcgag tctttagaga atcaatgggg
    23101 tatcgaagat agcgaaagtt attgtgcctt aatggagcat tttctttctg gcgaccatgg
    23161 agccaatacc tttaaagcaa acatggagga agccccagag caggttatcg ccttgttgaa
    23221 taaatttgct gtttttccct cagactatat ctctgattgc gctaataatc gctccggtaa
    23281 atcctcggcg aagctaatat gggcggcgga attatcatgg atgatctcga tatcaagcac
    23341 agcttttcaa aacgggacaa ttgaagaaga actggcctgg cattatataa tgcttgcttc
    23401 tcgaaaggcg cacgagttgt tcgaaagcga agaagattat caaaaaaata gtctaatggg
    23461 atttctttac tggcatatct gctgctatcg cagaaagtta acggatgctg aacttgaagc
    23521 gtgttatcgt tacgacaagc agttttggga gcactacagt aagaaatgcc gttggcccat
    23581 aagaaatgtt ccctggggag catcatccgt taaatactca taaacgcctg aaattttgcc
    23641 gggggcgctg cgcttgaccg gcctacatta gagcgtaact gcaatgaatt gtgcccatct
    23701 gtaggcctga taagcgtagc gccatcaggc atttacaact taaatcacac cctgcgccag
    23761 catcgcatcg gcaaccttca caaaaccggc aatgttcgcg ccctgcacgt agttggtttg
    23821 ctcaccttca ccaccatgct caacacaggc atggtggata tccagcatga tgtgatgcaa
    23881 acgtgcgtca actttctcgg ctttccagcc caggcgcgca gcgttttgtg ccatttccag
    23941 gcccgacgtt gctacaccgc cagcattagc cgctttaccc ggtgcaaaca gtacgcctgc
    24001 ctgctggaac agttcagtcg cttcgatggt ggtcggcata tatgcccctt cagcgacggc
    24061 tttaacgcca ttggcgataa gctgatgcgc ggcgtcaaca tccagttcgt tctgggtggc
    24121 gcaaggcagg gcgatatcaa ccggtacaga ccacggctgt tggccttcga gatagaccag
    24181 accaaattct ttggcgtaat ctgccgctcg accatcgcgg ctggctttga tttcgataag
    24241 acgtgccagt ttctctttcg tgaatccgct ttcatcaact acagtgccgc tggagtctga
    24301 cgcagtgatc acacgagcac caaattccat cgctttttcg atagcgtact gggcgacgtt
    24361 gccggagcca gaaacggaaa cgctcatccc ttcaaaaccc ataccgtggc gttttaacat
    24421 tgcttctgtg aaataaacca gaccgtagcc ggtagcttcc gggcgaataa gactaccgcc
    24481 aaatgaaagg cccttaccgg tgaagacgca ggcggtattg ttggagagct ttttcatcat
    24541 ccccgccata aagccgactt cacgaccacc aacgccgata tcacctgccg gaacgtcggt
    24601 atccgcgccc aggtggcgat acagttcagt catcagcgtc tggcaaaaac gcatcacttt
    24661 accttcgctt tttcctttcg gatcgaaatc gctgccgcct ttaccaccgc ccatcggcag
    24721 ggtagtcagg gcatttttga aggtttgttc aaagccgagg aatttgagaa tggaaaggtt
    24781 aactgacgga tggaagcgca taccgccttt gtacgggccg atggcagagc tgaactgcac
    24841 acgccatgca cggttgacct gtatctggtt gcgatcatca acccatacca cgcgaaactg
    24901 gatcacgcgc tccggttcaa ccagacgctc cagta
//
LOCUS       DAJTRW010000055        24540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRW010000055 DAJTRW010000000
VERSION     DAJTRW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..386)
                     /locus_tag="OO326_002435"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO326_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO326_002436"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO326_002436"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5966789.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO326_002437"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO326_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5966790.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO326_002438"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO326_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5966791.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO326_002439"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO326_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5966792.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO326_002440"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO326_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5966793.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO326_002441"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO326_002441"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5966794.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO326_002442"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO326_002442"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5966795.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO326_002443"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO326_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5966796.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO326_002444"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO326_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5966797.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO326_002445"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO326_002445"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5966798.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO326_002446"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO326_002446"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5966799.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO326_002447"
     CDS             10748..11212
                     /locus_tag="OO326_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5966800.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO326_002448"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO326_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5966801.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO326_002449"
     CDS             12236..13672
                     /locus_tag="OO326_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5966802.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO326_002450"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO326_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5966803.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO326_002451"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO326_002451"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5966804.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO326_002452"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO326_002452"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5966805.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO326_002453"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO326_002453"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5966806.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO326_002454"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO326_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5966807.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO326_002455"
     CDS             complement(20505..20798)
                     /locus_tag="OO326_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5966808.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO326_002456"
     CDS             complement(20795..22084)
                     /locus_tag="OO326_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5966809.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO326_002457"
     CDS             complement(22140..23078)
                     /locus_tag="OO326_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5966810.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO326_002458"
     CDS             complement(23098..23862)
                     /locus_tag="OO326_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5966811.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24180..>24540
                     /locus_tag="OO326_002459"
     CDS             24180..>24540
                     /locus_tag="OO326_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5966812.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAA"
BASE COUNT         6064 a         5931 c         6458 g         6087 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaagtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgattacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga actgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
    24001 gattgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt
    24061 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
    24121 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
    24181 tgtatcaacg ctgttttgat aatgccagtg aaacgctgtt tgtcgccggt aaaacgccac
    24241 ggctttcacg ttttgcattt agcgatgatc caaaatggga gtctggacat cacgttcatg
    24301 acaatgaaac cgagctgatt tacgtcaaga aaggggttgc aaggtttacc atcgattctt
    24361 cgttatatgt cgcacatgcg gatgacattg tggtgataga acgcggcagg ctgcatgcgg
    24421 tggcctctga cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt
    24481 ttcagggggc tgaggaaaat cagttactgc aaccgcattc ttgtccggta attgccgcgg
//
LOCUS       DAJTRW010000056        24344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRW010000056 DAJTRW010000000
VERSION     DAJTRW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(183..755)
                     /locus_tag="OO326_002460"
     CDS             complement(183..755)
                     /locus_tag="OO326_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5966813.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            973..1833
                     /gene="tesB"
                     /locus_tag="OO326_002461"
     CDS             973..1833
                     /gene="tesB"
                     /locus_tag="OO326_002461"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5966814.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1882..3168)
                     /gene="amtB"
                     /locus_tag="OO326_002462"
     CDS             complement(1882..3168)
                     /gene="amtB"
                     /locus_tag="OO326_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5966815.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3198..3536)
                     /gene="glnK"
                     /locus_tag="OO326_002463"
     CDS             complement(3198..3536)
                     /gene="glnK"
                     /locus_tag="OO326_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5966816.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3717..5498)
                     /locus_tag="OO326_002464"
     CDS             complement(3717..5498)
                     /locus_tag="OO326_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5966817.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5491..7263)
                     /locus_tag="OO326_002465"
     CDS             complement(5491..7263)
                     /locus_tag="OO326_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5966818.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7293..7751)
                     /gene="decR"
                     /locus_tag="OO326_002466"
     CDS             complement(7293..7751)
                     /gene="decR"
                     /locus_tag="OO326_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5966819.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7905..8723)
                     /gene="cof"
                     /locus_tag="OO326_002467"
     CDS             complement(7905..8723)
                     /gene="cof"
                     /locus_tag="OO326_002467"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5966820.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8823..10515
                     /locus_tag="OO326_002468"
                     /pseudo
     CDS             8823..10515
                     /locus_tag="OO326_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10580..11275
                     /gene="queC"
                     /locus_tag="OO326_002469"
     CDS             10580..11275
                     /gene="queC"
                     /locus_tag="OO326_002469"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5966821.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11327..11725)
                     /gene="fadM"
                     /locus_tag="OO326_002470"
     CDS             complement(11327..11725)
                     /gene="fadM"
                     /locus_tag="OO326_002470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5966822.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11819..12190)
                     /locus_tag="OO326_002471"
     CDS             complement(11819..12190)
                     /locus_tag="OO326_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5966823.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12341..14212)
                     /gene="ppiD"
                     /locus_tag="OO326_002472"
     CDS             complement(12341..14212)
                     /gene="ppiD"
                     /locus_tag="OO326_002472"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5966824.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14404..14676)
                     /gene="hupB"
                     /locus_tag="OO326_002473"
     CDS             complement(14404..14676)
                     /gene="hupB"
                     /locus_tag="OO326_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5966825.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14885..17239)
                     /gene="lon"
                     /locus_tag="OO326_002474"
     CDS             complement(14885..17239)
                     /gene="lon"
                     /locus_tag="OO326_002474"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5966826.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17427..18701)
                     /gene="clpX"
                     /locus_tag="OO326_002475"
     CDS             complement(17427..18701)
                     /gene="clpX"
                     /locus_tag="OO326_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5966827.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18827..19450)
                     /gene="clpP"
                     /locus_tag="OO326_002476"
     CDS             complement(18827..19450)
                     /gene="clpP"
                     /locus_tag="OO326_002476"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5966828.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19697..20995)
                     /gene="tig"
                     /locus_tag="OO326_002477"
     CDS             complement(19697..20995)
                     /gene="tig"
                     /locus_tag="OO326_002477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5966829.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21339..21656)
                     /gene="bolA"
                     /locus_tag="OO326_002478"
     CDS             complement(21339..21656)
                     /gene="bolA"
                     /locus_tag="OO326_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5966830.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21961..22539
                     /locus_tag="OO326_002479"
     CDS             21961..22539
                     /locus_tag="OO326_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5966831.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22583..24058
                     /gene="ampG"
                     /locus_tag="OO326_002480"
     CDS             22583..24058
                     /gene="ampG"
                     /locus_tag="OO326_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5966832.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5717 a         6703 c         5864 g         6060 t
ORIGIN      
        1 ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg
       61 tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac
      121 tggatcggtc aactgatcct taactgatcg gcattactcc gggcggagag ggttaacgtc
      181 gcttaatatt gcgtaggagc gggaactgcc gaagacggat tcacctgagt tggtgaagtc
      241 gaaggtacgg tagtcgttgc gccaccgctg gcctgaaccg gcacggcggt ttgctgtacc
      301 ggcaccaatg tcaggtcggc tttagttccg ccctggttga tcaccggctg aacggtatcg
      361 gtgataaata ccagtttgtc attcacggta atcgccgcac tcaacagaat acgcgcgttc
      421 ggctgaacat ctgccgggtt aaatggcaga acaaagctga atggtgactg tttaccttca
      481 gtacgcaccg ctttctgcgc cagcactttt gacggtgcat cggctaacga cgcgtcagaa
      541 agtgtcacgg tcagcacagc atcaggcggc agtgcgactt tctgacggat ccagacagta
      601 ccggagacat tcggttgctg gatagcaggt tgttgtgttg ctgaaataga cgtatttgca
      661 gccggggctg gcgtctgaat atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca
      721 accgctaaac cactggccat gtgcacgagt ttcattcatt tctccttatt atcaatgcac
      781 cagcgggcta actttcctcg ccggaagagt ggttaacaaa atagtaacgt caacaagtgt
      841 ggcacacatc acgcatttct gcctgtaatt agcccgtaat tcagactatt tcacccatcg
      901 gaccagtggc aaatttgcgt tatactcaac tcactttggc ttgctgcggc agctttgtta
      961 ctggagagtt atatgagtca ggcgctaaaa aatttactga cattgttaaa tctggaaaaa
     1021 attgaggaag gactctttcg cggccagagt gaagatttag gtttacgcca ggtgtttggc
     1081 ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga agagcggctg
     1141 gtacattcgt ttcacagcta ctttcttcgc ccaggcgata gtaagaaacc gattatttat
     1201 gatgtcgaaa cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc tgctattcaa
     1261 aacggcaaac cgatttttta tatgacggcc tctttccagg caccagaagc gggtttcgaa
     1321 catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc
     1381 gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt
     1441 ccgctggaag tccgtccggt ggagtttcat aacccactga aaggtcacgt cgcagaacca
     1501 catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag
     1561 tatctgctcg gttacgcttc tgatcttaac ttcctgccgg tagctctaca gccgcactgc
     1621 atcggttttc tcgaaccggg gattcagatt gccaccattg accattccat gtggttccat
     1681 cgcccgttta atttgaatga atggctgctg tatagcgtgg agagcacctc ggcgtccagc
     1741 gcacgcggct ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt tgcctcgacc
     1801 gttcaggaag gggtaatgcg taatcacaat taaaaaaata gccggaggtg aaaaccgtcc
     1861 ggctattttt tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc tgttgacgtc
     1921 cagcccttct cgctcttgtt cttccggcac acgtaggcca accgtcagat ccgccagttt
     1981 gtagccaata aatgccacca caccggacca gacgatcgta atggcgatgc tttccaactg
     2041 taccagcaac tgatggccca tcgtcacacc ttcagcgaag cccacgccgc ccagagaact
     2101 ggcggcaaaa atcccggtca tgatacagcc gacaatgcca caaacgccgt gcacaccgaa
     2161 gacatcgcag ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc cccacaaacc
     2221 cgccagacca gctaccacgc cgataatcaa cgcgccgcca accccaatgt agccgcacgc
     2281 tggcgtcacg ccgaccagac cggcaatcgc gccagaacac gctcccagca gagaaggctt
     2341 accacgcagc gcccattcac cgaagatcca gccaagaatt gccgccgccg ttgcgaccac
     2401 agtattcaca aatgccagtg ccgcgatttc attcgccgtg cccactgacc cggcgttaaa
     2461 gccaaaccaa ccgatataga gaatggcagt cccggtgaag accatcggca ggttgtgcgg
     2521 tttaaacgcc tctttaccga agcctacgcg tttgcctatc agatacgcgc ccaccagacc
     2581 ggcgattgct gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg caccgtgaga
     2641 agccagcaaa ccaccgcccc acaccatatg cgcaatcgga atgtaagaga gcgtcagcca
     2701 taccaccacg aaaatcaaca cagctgagaa gcggattcgt tccgccagcg ccccaactat
     2761 caagcctacg gtaatgcagg caaacgatcc ctgaaacgct acgtggatat actgataaat
     2821 gctgcccatc accgccgtca gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa
     2881 gaagttgttg ccctcaccaa acgccagcga ataaccgtaa accacccaga gaatacagac
     2941 cagtgcaaac gtcaccgtca cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa
     3001 cccaccgtaa aacagggcaa tccccggaat agtcataaac agcaccagcg cagtacaaat
     3061 catcataaac gcattgtcgg ctttatcggc caccgcaggt gcagccatta ccagtcccgg
     3121 cagcatcgcc agtgaagcaa gcccagtttt tatcgtcgct atcttcattt ttcgttcctg
     3181 ttgctgtgtg ccagagatta cagcgccgct tcgtcggctt cgccggtacg aatacgaatg
     3241 acgcgttgca attcagcgac gaagattttg ccgtcgccaa tttttccggt gtaagccgcc
     3301 ttactgacga tatcgatcac ttcatcgagt tggtcatcag caatcgccac atcaattttt
     3361 acttttggca ggaagttgac gctgtattcc gccccccggt acagctcggc atgccctttc
     3421 tgacgcccga aacctttcac ttcggtgacg gtcaggccct gaataccaat ggaagataac
     3481 gcttcacgaa cgtcttccag cttgaatggt ttgattatca cggtcaccag cttcatagat
     3541 cccctccggt cagaattcgg taatggttct gctacacgaa gaaggtattg caagcggtgt
     3601 gccagaaatg aaaaacgaga aagagagcag gaagttaaga agattgaaaa tgaaaacgca
     3661 ctatgacagt gcacagtgcg ttacattttt gcaccaacga tgaaatattg cgctattcag
     3721 gcgctcaatg attcctcttc acgcacgctg gctgccagct cttcgcccgc aagttgcagt
     3781 tgatacatct gccagtagcg tccctgggcc gccagcagtt gctggtgagt gccctgctcc
     3841 acggcttgcc cacgatgaag caccagaatg gtgtcggcat caataatagt tgataagcgg
     3901 tgagcaatca ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg ttgaatcgcc
     3961 tgttcagtac cggagtcaat gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc
     4021 tccaccagca cgcgcgccag tgccagcagt tgcttttgcc cgactgagag attatttccc
     4081 tgctcgccca gcggcgtata aatacaatca ctcatgctgc gcgccagctc cgccagttgc
     4141 acggtttcca gcgcctgcca gacgcgttct tcggagatat cccgccccag cgtcacatta
     4201 gcgaggaagg tatccgccag caccaccgga tcttgctgca ccattgccac gccctggcgc
     4261 agcgcgctat gacttagcga acttaatgga cgaccatcaa ggtgaatctc accttccgtt
     4321 agcgggtaat agcccatcaa taaactggcg agggtacttt taccactgcc ggtatgcccg
     4381 accagcgcca caaagttacg cgaaggcaca gagagattaa tgttctttag caccagattg
     4441 tcatcgcgat aagcaaatga cacgttatcg acttcgatgg tgccactctg taacgggtaa
     4501 tcatcattgc catattgctg gcgcggtccg tccatcagtt caaacacgcg ctcaccagca
     4561 acaacagcct gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg ttcgttaagt
     4621 cgcccaagat agctgataaa cgcatacaac acgcccactt caatggtgcc gctggcggag
     4681 aagccaaaca gcatcaataa gccacaaaga atgagcgatg aaaacagact cagcagcgga
     4741 cgcagcagaa aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg tgaacgactg
     4801 gcctccccca tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat aacgctcatg
     4861 ccattgatga tttcgttaaa gccgtcgttg atatccgcca gataggcgcg cacacggcgg
     4921 acaatcggcg tgctgtaacg ctggtatatc accattacca ccagcaccac cgggaaaatc
     4981 attatcgcca ccagcgccat tcgccagtcg aggctgaaca tcgccaccag catcgcgccc
     5041 accagtgcgg cactgcgcag gacagttgcc actacggtaa cgtagagatc gcggatcact
     5101 tcagtgtcat tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt
     5161 aatggctggc gtaacgcagc atccatcacg tcggtacgca actgttgcac tgcgcctact
     5221 gccgcccgat taaacagcag cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc
     5281 ccaacatacg ccgcagccag ccctgcaacc actttcaacg gcaggttatt tttcgctacc
     5341 atattgtcga taaaatagct gataagcagc ggcccactga cttctgccgc cgccgcaacc
     5401 cacatcatca ggaccgcaat ccccagcggt ttacgccacg gcgaaccata cgctaacagg
     5461 cgcttgagag tcggccacag ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc
     5521 cggaacgtcg tcgagcgccg cctccagttg ttgatagcga tacatatcgc gataccaacc
     5581 gctttgttgc gccagctcat catgattgcc acgctgggcg atatgtccgt gctgcatcac
     5641 aataatttca ctggcttccg tcagtgccga aaggcgatgg gcactgatga ttaccgttct
     5701 tccctgtccc cactgacgca ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc
     5761 cgaaagcgca tcatcaagga tgaggatttc cgcgttgact aataacgctc gagcaatgga
     5821 gatacgctgt ttttgcccac cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta
     5881 accttgcggt agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga catgctcaat
     5941 ctcttgctgg gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg tgtcagaaaa
     6001 aaggaatggc gtctggctga ccaccgccag gcggctacgc cagctatcga gttgtaactt
     6061 cgtcagagga atatcatgaa agcgaatatc cccctcgctg acgtcgaaat gacgctgaat
     6121 gagcgacaac agggtacttt tgccggaacc agtcggcccg caaataccca gcatctgacc
     6181 gggtttcagg gcgaaattga cgttttccag cgcaggatgg tcagtctgcg gatacgtgaa
     6241 ctggtgaata tttacatcca gttcgccacg cccttccggc acgggttcgc taccatcgtt
     6301 caccaccggc gcttccgcca gcatggcgcg aatacggctg tatgcagcac taccacgttc
     6361 cacaatgtta aacatccatg ccagcgccag cattggccaa atcatcagac ctaaatacat
     6421 cataaaactg gtgagctggc ccagcgttaa actgccctgc accaccatcc aactaccgcc
     6481 gccaatcgcc agcaagttcg ccataccaat cgcgatatag atggtcgggt cgaagcgagc
     6541 atcaatacgc gccacccgca tgtttttttt gccggtatct tcggcatccg cggcaaataa
     6601 cgccgactgg cgatcttcca gaccaaaggc tttgatcatg cggatactgg tgaggctttc
     6661 ctgggtgcgg tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa
     6721 ggcatcgccg ttgcgcttaa tcatgatcgc catcactggc atcggtaaca gggcaaataa
     6781 ggtcaactgc cagctaattt gcgtagacat cataatcaac acagcgcagc ccattaccag
     6841 tgaatccacc agcgtcagca ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt
     6901 ggtcgcacga gccatgagat cacccgtgcg atgacgcagg taaaactccg ggtgctgccg
     6961 gcttagctga cggtaataat cttcacgcag ttcaacagcc agttggtaag acgcgccaaa
     7021 cagcaatacg cgccagacat aacgcaggag ataaaccaca acggcaatca gcaccatggt
     7081 ggcgatccac atcaggctca gcccggtagt aaagtgttgt tctgtcacgc catcgacaac
     7141 aataccaacc acttttggcg gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac
     7201 agccccaaga tagcgacgcc attcccgacg gaaataccag cttaattgag caaataatcg
     7261 cacgcgttgt gtcctgacct gattctggat atttattcga tgggtaaaga agtggtgtat
     7321 ttaatctgtt ccatcgcgaa gctggaagtg acgtccgaca gccccggcac gctgtttacc
     7381 agacgcttat aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg catcagataa
     7441 tcgtattcac cagccatgcg ccagaccccc agcacttctg gcatttcggt aaccaccgtg
     7501 acaaagcggc aataccattc gctgctgtga tgttgcgttt ttatcagcac aaaggcggtc
     7561 aggccgaggc ctattttttc cggatccagc agggcgactt tgccgataag gataccgtcg
     7621 tcctccagcc gtttcaggcg cttccagcag ggggtggttg tcagattaac ggcttcagcc
     7681 agtgcctgca aagagagggt gcaatcctgc tgcagtaagg ccagcagctt acggtcaatt
     7741 ttatctaaca tagcccttcc acggagaatt tttttctctt ttaattctat tttaaaggtg
     7801 aaatcgcgac aatttattct gtgcggttta aacaccacgg cacaaaatga caaattcaac
     7861 gcaaaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc gggggaataa
     7921 ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg atttcggcaa
     7981 tgtccaatca ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc attgcccata
     8041 ataaacccgc taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc
     8101 atgcaatcgc gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc agcgccttta
     8161 ttgcagccca ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa atgtgcacgc
     8221 tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag
     8281 aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat ctgataacga
     8341 aaaccgctat agacaaatac ctgcaacaac gcagggctct ctttcccggt aaaccaaccg
     8401 tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag caccagctcc
     8461 gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga atgcacgcgc
     8521 gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat atgctgcatc
     8581 tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc
     8641 aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt gccatccata
     8701 tcaaatgcta ccagacgagc catttctttc tccactctgt gacacggtta atggtgattg
     8761 agtatcacct gatatatacg gaagtaatag tgaatagtta aataatattg ttccgggttt
     8821 atatgcgatt gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tcagccgcaa
     8881 accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg
     8941 ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga
     9001 aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa
     9061 caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaggt
     9121 gagctgcgca ctctgttaca gccatttatg ggcggacaat ggcaaaacga tacacccacg
     9181 ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt
     9241 gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact
     9301 cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacag gttacgctgg
     9361 gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac
     9421 ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc
     9481 gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat
     9541 tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca
     9601 ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag
     9661 agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact
     9721 ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc
     9781 ggcaaaccgg aggagctgca acgggtcagc caagtcagta gtggcatcag tttaggtttt
     9841 tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc
     9901 tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc
     9961 gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc
    10021 aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca
    10081 gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac
    10141 gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga
    10201 ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg
    10261 ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa
    10321 gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg
    10381 gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc
    10441 gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc
    10501 gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat
    10561 tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc
    10621 caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga
    10681 ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg
    10741 agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct
    10801 gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac
    10861 gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt
    10921 aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg
    10981 ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat
    11041 tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta
    11101 ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg
    11161 cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct
    11221 ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt
    11281 ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct
    11341 gctccagctt ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa
    11401 tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga
    11461 cctggtttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca
    11521 ggtcgcttaa taccgctgga cgacgatagt taatattgat attgaccacg acgaaggcga
    11581 tgttatgggc cgtcatccac tgaaaactgt cgctattttc caccccatcc cagcgggctt
    11641 cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc
    11701 cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag
    11761 aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt
    11821 acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct
    11881 ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg
    11941 ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa
    12001 tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg
    12061 ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca
    12121 gcgcgctatg agacattccg gcacaggcca gggacagtgt aatgagcagt gctttaattc
    12181 cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa
    12241 aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg
    12301 ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat
    12361 cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt
    12421 gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac
    12481 cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac
    12541 cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt
    12601 cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag
    12661 cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct
    12721 gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg
    12781 gtttcaccgc ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg tcgccgtcta
    12841 cggtgatgat gtcagagttg atgcccgacg cgccgttttc acctaccaga ccgccgttaa
    12901 agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc
    12961 aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt
    13021 cgttgcttgc cgcatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt
    13081 cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg
    13141 gctgaatgtc gtccagacgt acaatcagga aaccgaccga agatttgatg acaccagaca
    13201 gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta
    13261 accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta
    13321 atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag
    13381 ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct
    13441 gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca
    13501 gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt
    13561 agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga
    13621 tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac
    13681 ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt
    13741 tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat
    13801 agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt
    13861 taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct
    13921 cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg
    13981 ccagctcgga gtattgatcg cccagctgtt gctgcatacg attacgctcg ctgttaaagg
    14041 cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc
    14101 ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga
    14161 aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac
    14221 aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta
    14281 ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa
    14341 ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa
    14401 cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc
    14461 agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt
    14521 aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt
    14581 tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc
    14641 tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta
    14701 tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc
    14761 cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa
    14821 aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag
    14881 gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca
    14941 gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa
    15001 tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat
    15061 gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga
    15121 tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg
    15181 cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt
    15241 ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg
    15301 cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg
    15361 tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc
    15421 ccacttccgt ccacgccaga ccgattacct gaccgacacg gttttcgtta tcagcgcgac
    15481 catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat
    15541 gttttaatga cttatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt
    15601 cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg
    15661 cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca
    15721 gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca
    15781 cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca
    15841 tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct
    15901 ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt
    15961 tgtcgatctc atcgagcagg aacagcggat ttttcacgcc cactttcgcc attttctgga
    16021 tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat
    16081 cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact
    16141 gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga
    16201 ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca
    16261 gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc
    16321 tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt
    16381 ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg
    16441 cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggca
    16501 cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact
    16561 cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca
    16621 gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg
    16681 acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat
    16741 ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt
    16801 ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct
    16861 gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc
    16921 cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg
    16981 tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat
    17041 cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat
    17101 catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga
    17161 tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt
    17221 cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg
    17281 acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc
    17341 cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg
    17401 ttaactaatt gtatgggaat ggttaattat tcaccagatg cctgttgcgc ttccggctta
    17461 ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt
    17521 tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg
    17581 gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc
    17641 gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg
    17701 gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc
    17761 aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga
    17821 tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt
    17881 accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc
    17941 agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat
    18001 tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc
    18061 agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg
    18121 tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc
    18181 tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc
    18241 acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg
    18301 cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg
    18361 ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg
    18421 ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc
    18481 aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact
    18541 tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac
    18601 acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa
    18661 tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt
    18721 gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg
    18781 ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg
    18841 tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc
    18901 gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat
    18961 tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc
    19021 cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt
    19081 taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga
    19141 tggtgctgac atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga
    19201 tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca
    19261 ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa
    19321 aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg
    19381 tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc
    19441 tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc
    19501 ttatggacgg attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc
    19561 ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa
    19621 aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg
    19681 cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt
    19741 cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc
    19801 gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt
    19861 cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcgtca gctttcagct
    19921 cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt
    19981 cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg
    20041 cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt
    20101 cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct
    20161 tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag accttctacg gaaccatctt
    20221 caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt
    20281 tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt
    20341 ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt
    20401 caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc
    20461 cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt
    20521 caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc
    20581 cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac
    20641 cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca
    20701 gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga
    20761 tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc
    20821 cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta
    20881 cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga
    20941 tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt
    21001 tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt
    21061 cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa
    21121 gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt
    21181 gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa
    21241 aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatctttagc
    21301 aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca
    21361 cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa
    21421 gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc
    21481 attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta
    21541 aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg
    21601 aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa
    21661 tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg
    21721 tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta
    21781 tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg
    21841 ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg
    21901 caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac
    21961 atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa
    22021 ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg
    22081 atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa
    22141 gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgctctgcg tttcctgctg
    22201 caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg
    22261 gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc
    22321 tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa
    22381 atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa
    22441 aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac
    22501 accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg
    22561 caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg
    22621 ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg
    22681 caccttacag gcctggatga cggtcgaaaa tatcgatctc aaaaccattg gtttcttctc
    22741 tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac
    22801 gcccccattt tttgggcggc gacgcggttg gctgctcgcc acgcaaatcc tgttattagt
    22861 cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct
    22921 ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac
    22981 cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg
    23041 tttagggatg ctggtttccg gcggcctagc cctgtggttg gcagataaat ggttgggctg
    23101 gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct
    23161 tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc
    23221 acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct
    23281 gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt
    23341 cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat
    23401 tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct
    23461 gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga
    23521 taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg
    23581 cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca
    23641 atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg
    23701 ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt
    23761 accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga
    23821 caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc
    23881 ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt
    23941 gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg
    24001 cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc
    24061 tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc
    24121 agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat tattgacgcc
    24181 atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc
    24241 cagttgtcac tttaatgata attatttgtt aaataattgt tttatttcgc attggttata
    24301 ccaattgccc accaagactc cttctgcact ccccttttgt tata
//
LOCUS       DAJTRW010000057        23874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRW010000057 DAJTRW010000000
VERSION     DAJTRW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(32..904)
                     /gene="rluF"
                     /locus_tag="OO326_002481"
     CDS             complement(32..904)
                     /gene="rluF"
                     /locus_tag="OO326_002481"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5966833.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1252..2199
                     /locus_tag="OO326_002482"
     CDS             1252..2199
                     /locus_tag="OO326_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5966834.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2269..3075
                     /locus_tag="OO326_002483"
     CDS             2269..3075
                     /locus_tag="OO326_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5966835.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3085..3492
                     /locus_tag="OO326_002484"
     CDS             3085..3492
                     /locus_tag="OO326_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5966836.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3492..3986
                     /locus_tag="OO326_002485"
     CDS             3492..3986
                     /locus_tag="OO326_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5966837.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4052..4849
                     /locus_tag="OO326_002486"
     CDS             4052..4849
                     /locus_tag="OO326_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5966838.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4860..5648
                     /locus_tag="OO326_002487"
     CDS             4860..5648
                     /locus_tag="OO326_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5966839.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5700..6929
                     /gene="sorE"
                     /locus_tag="OO326_002488"
     CDS             5700..6929
                     /gene="sorE"
                     /locus_tag="OO326_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5966840.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7311..8000
                     /gene="pepE"
                     /locus_tag="OO326_002489"
     CDS             7311..8000
                     /gene="pepE"
                     /locus_tag="OO326_002489"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5966841.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8091..9722)
                     /locus_tag="OO326_002490"
     CDS             complement(8091..9722)
                     /locus_tag="OO326_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5966842.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9942..13625)
                     /gene="metH"
                     /locus_tag="OO326_002491"
     CDS             complement(9942..13625)
                     /gene="metH"
                     /locus_tag="OO326_002491"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5966843.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13825..14649
                     /gene="iclR"
                     /locus_tag="OO326_002492"
     CDS             13825..14649
                     /gene="iclR"
                     /locus_tag="OO326_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5966844.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14966..17150
                     /gene="espL4"
                     /locus_tag="OO326_002493"
                     /pseudo
     CDS             14966..17150
                     /gene="espL4"
                     /locus_tag="OO326_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17119..18856)
                     /gene="aceK"
                     /locus_tag="OO326_002494"
                     /pseudo
     CDS             complement(17119..18856)
                     /gene="aceK"
                     /locus_tag="OO326_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19040..20344)
                     /gene="aceA"
                     /locus_tag="OO326_002495"
     CDS             complement(19040..20344)
                     /gene="aceA"
                     /locus_tag="OO326_002495"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5966845.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20374..21975)
                     /gene="aceB"
                     /locus_tag="OO326_002496"
                     /pseudo
     CDS             complement(20374..21975)
                     /gene="aceB"
                     /locus_tag="OO326_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22244..23173)
                     /gene="metA"
                     /locus_tag="OO326_002497"
     CDS             complement(22244..23173)
                     /gene="metA"
                     /locus_tag="OO326_002497"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5966846.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23330..23773
                     /locus_tag="OO326_002498"
     CDS             23330..23773
                     /locus_tag="OO326_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5966847.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5875 a         6093 c         6055 g         5851 t
ORIGIN      
        1 tttggacgtt ttcatcagtc tgaggcgtta atcagcgtcc cttcttttta cgccccggcg
       61 aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt ttttccatct
      121 taacgactgg acgtttgatg cccgctgttt tcggtttggc cttcgcttta ggttttgcct
      181 cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc
      241 gccactcccc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta cgttccagct
      301 ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc agcccctgca
      361 ctagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt ttcgtcaccg
      421 tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta atcggtttat
      481 cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc ttattcacca
      541 gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg cggccaatcg
      601 ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg
      661 tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct tcggcttccc
      721 gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc tgatcgccaa
      781 tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga tccgcttcgc
      841 ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat gagtcgggca
      901 gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga aaaacagggt
      961 tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc
     1021 ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta cgaatcgctg
     1081 cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc
     1141 tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt tatatcagat
     1201 caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac
     1261 agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca ggatatgacg
     1321 caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg cttgcttaaa
     1381 cgaggccgcg atcagggaat ggtcaccatc gccatcaact atgactacaa cgaaaatctc
     1441 tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga
     1501 aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc gcaactgctg
     1561 gatcgcttgc tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca
     1621 ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt accaattatt
     1681 ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac ctacagcgcg
     1741 gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggctctgct ggataaccca
     1801 ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc ctactgggat
     1861 aatctggata tcgccctggt gggaattggc tcaccggcca ttcgcgacgg cgctaactgg
     1921 catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt tgctggcgat
     1981 atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat gagcgaaaaa
     2041 acgctctcta tcgaaatgaa taaattaaag caggcgcgat attccattgg cattgccatg
     2101 agtgaagaaa aatacagcgg aattattggt gcactgcgtg gaaaatatat taattgtctg
     2161 gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg atgtttaccg
     2221 taataaaaat tacggcgggt ccctattatc taaaatcagg agtctgttat gcaaacgtgg
     2281 ttaaatttgc aggataaaat cattattgtc accggcggcg catccggtat tggtctggcg
     2341 attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat tcacggtggc
     2401 gatggtcaat atgaaggtca taaaggctat cagttttggc cgaccgatat ttctagcaca
     2461 aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat cgacggtctg
     2521 gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg
     2581 cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt
     2641 tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg
     2701 aatgtttcct cagaaagtgg gctggaaggc tcagaaggac aaagctgtta cgccgctacc
     2761 aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa acacggtatc
     2821 cgtgtggtcg gtatcgcgcc cgggattctg gaaaaaaccg ggctacgcac gccggaatat
     2881 gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga aggctatacc
     2941 aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtagctga ttttgtttgt
     3001 tatctgttgt ctgaacgcgc cagctacatc accggagtaa ccactaacat tgcgggcggc
     3061 aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgccca cggcaaatta
     3121 gcttgcgcca tgctggaatc ggttcagatg gtttacggca acgccaatgt tgaagccgtt
     3181 gcatttgtgc ccggtgaaaa cgccggagac attgcggtaa agctggaaaa gttagtaagc
     3241 attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag cccgtggaat
     3301 gcggcagcaa tgcttgcgat gcgcaatcct cgtttgcggg taatcagcgg cctttctctg
     3361 ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga actgtgtgaa
     3421 cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc aacgacagag
     3481 gaggatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc cacggccagg
     3541 tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc aatgacgaag
     3601 tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg
     3661 ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa tatcaggacg
     3721 aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt cgccaggggg
     3781 taaaaatagg caccctgaat attggcggca tggcctggcg gcccggtaaa aaacagttaa
     3841 ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta aataatcttg
     3901 gtgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa
     3961 taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc tttaacaggc
     4021 agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac aaatcattgc
     4081 tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat ttcagactca
     4141 tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa aaaccggaat
     4201 aatactcggc ggtacgctgg aattgatagc tctctgctgg atgaacgtcg gcgcggcgca
     4261 atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg ttggtcagca
     4321 aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac
     4381 ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga
     4441 ggcgcggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc aggcgctgcg
     4501 cgtcgccatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg tcagcaatat
     4561 gttaaacgcc attcccgaat ttgtgacccg aggactacag attgctggcg gttttatcgt
     4621 ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga tgcctttctt
     4681 tttcctcggt ttcctcgcag gtggctacct cgatctcagc ctgctggcgt tcggtggcgt
     4741 cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggtgta aagctgaacc
     4801 tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac ggagccatca
     4861 tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc
     4921 aacaggcgtc ctttaacttt gaacgtattc acgggctggg cttttgctac gacatgatcc
     4981 ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc aggctacacc
     5041 tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgcg gaaattgatg
     5101 acggtaccat caacggcatc aaagtcggtc tgatgggacc attggcagga gttggcgatc
     5161 cactggtctg gggaacgctg cgcccgatta ccgccgcgct cggcgcatct ctggcacttt
     5221 cgggcaacat tctcggcccg ctgctgttct tctttatttt caacgcggtg cgtctggcga
     5281 tgaagtggta tggcctacag ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg
     5341 gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg
     5401 gcgtgctggt gaccaaatgg acgtcaatca acgtaccgtt ggtggtttca caaacgcatg
     5461 ccgccgatgg ctccaccgtc accatgaccg tgcagaacat tctcgaccaa ctttgccctg
     5521 gtttgctggc gctcggtctg acgctactaa tggttcgtct gctcaacaaa aaaattaacc
     5581 cggtatggct gattttcgcc ctgtttggct tagggattat cggcaatgct ctgggcttcc
     5641 tgtcctgatt cttcgccccg gcacgactgc cgaggccatc gctcaacatg aggtggttta
     5701 tgaaaacaac agctctgcgt ctttatggta aacgtgattt acgcctggaa acctttgacc
     5761 ttcctgaaat gcaggaggat gaaatcctcg caacggtggt cactgacagc ctgtgcctct
     5821 cttcctggaa agaggccaat ctgggtgaaa accataaaaa agtacccgac gatgtgacga
     5881 ccaacccaat catcatcggc cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt
     5941 ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg caactccccg
     6001 accgcccgga ttgccccggc tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg
     6061 ttattcccaa cgaggtcatg gaacaatatt gcctgctggc atacgacggc gaaacctatt
     6121 ttgaaggctc actggttgaa ccgctttcct gcgtgattgg cgcgttcaac gccaactatc
     6181 atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg cacacgctga
     6241 tcctcggcgg caccggacca atgggactgt tggcgattga ttatgcgctg catggaccag
     6301 ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt tatgcgcgca
     6361 agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc gatgcagcat
     6421 ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc gtctttgtgc
     6481 ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt tgcctgaatt
     6541 tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac gatgtgcatt
     6601 atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg
     6661 tcaaactgat cgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc
     6721 tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg
     6781 tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat caagcactgg
     6841 cggcgattct ggcgcgtcat caggggatct ggtccggtga ggtggagcaa tacctgctcg
     6901 cccatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat gtcgctggct
     6961 ggtcgcccca gcaacttcgc gaccggatgt gcggctgatt gctgaagcag cacaatttgc
     7021 acgagaagga taaaaaatat cagcgcctta aggggcgctc aaattggtgg caaagtacac
     7081 attgtttatg ccggatgcgg aatgaagcct tattcggtct acataatttt gaaaatccaa
     7141 tatattgcag ttatctggta ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt
     7201 catcagtctc agcgcccggt gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa
     7261 attcgcagtg gtagcatgcc ttccgtgatt tcttactaac cggagaagta atggaactgc
     7321 ttttattgag taactcgacg ctgccgggta aagcctggct ggaacatgca ctgccgctaa
     7381 ttgctgaaca gttgcagggt cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc
     7441 agacctggga tgattacaca gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg
     7501 tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga
     7561 ttgtcggcgg cggtaatact ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg
     7621 ctccaattac tgacgtggtt aaacgtggcg cgctgtatat tggctggagc gcaggcgcta
     7681 accttgcttg cccaaccatt cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt
     7741 tcgatgcgct aaatctgttc ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg
     7801 aaggccataa aggtgaaacc cgtgagcagc gtattcgcga actgctggtc gtcgcgccag
     7861 aactgacgat tattggtctg ccggaaggta actggatcac agtgagtaaa ggtcacgcta
     7921 cgctgggtgg cccgaacacc acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg
     7981 aagctggtca ccgtttttaa gtgaattgcc ggatgcatga tccggcatct tgttcatagt
     8041 gcctgatgcg acgcttgacg cgtcttatca ggcctacagg attcacaata ttaatactcg
     8101 tcccgtcctt catcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc
     8161 gagttcaggc gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatgctc
     8221 tgcacgtttt gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg attaagaatg
     8281 cgaaaacgat gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc agagaagaac
     8341 accgacattg ccagctttaa attactgagc aattgctcat agagcgcatc cagttccttc
     8401 aacccatcaa gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc
     8461 atgcgctcga cgatatcgga ggcctgttca aggttgagcg acatttcgat gatctccgcc
     8521 cagcggcgcg actcttcctc cgccaactct tcttttggca tccgcgccag atacagctta
     8581 atggcggtgt agagaacgtt gatatcatct gccagcttac gcagctcttt ctcctgccgt
     8641 ggctcgccgt gcatcacttt attcagccct tccatcatct gttccatggc gtcgccaatg
     8701 cgcagggttt cgcgcgcggc gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca
     8761 tccagatgtt taggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg
     8821 caaaaccgtg ccatcgggtc aacaaatggc agcatgacca ggcaacgtac aaggttgtag
     8881 aagacgtgga aatagatcac cagttccgct tttggcaatg acagcttccc cattgtctct
     8941 gccagcaaat ggacaaacgg caggataatc aggctaccca ccagcttaaa cagcagacta
     9001 cccagcgcga cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcagg
     9061 ccggaaccga ggttagctcc aatcaccaga cagagcgcca cggggaagga gataatgcct
     9121 gcagcggtca gagtcgcggt cagcagtaca gcagcaaggc tggagtagct gataatggcg
     9181 aacatcgcgc caatcagcgc atccagcaga atatcgccgg tcagcgaggc aaagatcacc
     9241 tgaacgccgt ttgcctgggt gatcggcgtt acggcctgca caatcaactc cagcgccagc
     9301 aaaatcagcc caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt
     9361 ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg acagccagga taagtcgaag
     9421 gtgagaatac gcgccattag cgccgtcccg acatctgccc ccagcacaat gaccagagcc
     9481 ggtgcgaggg ctaccagatc ctgagcgacg aacgaggtca ccagcatggt ggtggcatta
     9541 ctgctctgta ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg
     9601 acgctccggc taaggacagt acgcaaacgc gcgccgaaga cgcgcattac gccggttcga
     9661 acaatatgag tcccccagac cagcagggcg acggcagaaa gcaggtgaag cagcgttaac
     9721 acgagttcag attctcctta tcgttatatg ttcctgaagc aattgaatga ccaaaggacc
     9781 ggaaacacgc tgactggcat ctttactgtg ccttttgaca ttcgtttttc cggctaagat
     9841 tattccagcc tgtttccagt ataagtgttt catattaata aagagacagg gcactcattg
     9901 agcgccctgt ttgttgtaag gaaaagtgac agatttgtga atcagtccgc gtcatacccc
     9961 agattcggtg ccagccagcg ctcaacatcg gagacgctca tacctttacg gcgggcatgg
    10021 tcttcaacct gatcgcgctg aatttgcgcc accgcgtagt acttgctgtc cggatgactg
    10081 aaataccaac ccgaaaccga tgcaccaggc cacatcgcga aagattctgt gagtttcatg
    10141 ccagtatgtt tttccacttc cagcagctcc cagatggtgg ctttttccgt atgttccggg
    10201 caggccggat agcccggtgc cggacggatg ccctggtagt tttcgcggat cagttcttcg
    10261 ttgctgaggt tctcgttcgg cgcatagccc cagtagactt tacgcacacg ctcatggaga
    10321 tactccgcaa aggcttcggc taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa
    10381 tcatcgtgct gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc gccagtcacg
    10441 gcaaatgcgc cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga
    10501 cagtagttag cgaatcccgt tttttcggtc tgttgacgca gatggtggct gacgttgatc
    10561 acatgggtgc gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg gtttgccggg
    10621 aacaggccca ccacgccacg cggattcagc gttttctcgg cgcttaattt atccagcatg
    10681 tcgttggcgt ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg
    10741 cgaggatact tcccggccag cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta
    10801 cgcagcgttt cgatgctggc ttccacttcc tgcacgccga gacggtgcgc caccggcggc
    10861 gtgtaagcct gccagtcaaa tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt
    10921 ggtgtgcgtg gtttcttgcg cccgtgctga atacgcacgg tttcgtactc cttgcaggta
    10981 cgagcgacaa aatcatcacg ctgagtatcg gaaagcagcg ccgctaccac accaacggtg
    11041 cgcgaggcat tctgcacata caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc
    11101 gtgtgcgctt ttgaggtggt cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc
    11161 tccatctctt tcgccacgtt aaccatctcg tccagcgacg gcgtgataag ccccgaaagg
    11221 ccaatcaaat cagcattcac ttctttggcg atacggagaa ttttttccgc aggcaccata
    11281 acgccgagat cgacaatttc gtagttgtta cattgcagca ccacaccaac gatattttta
    11341 ccgatgtcgt ggacatcgcc tttcacggtg gcgatcacca tcttgccgtt ggttttaccc
    11401 tgctctttgc tggcttcaat aaacggttcg agatacgcca ctgcctgttt catgacgcgc
    11461 gccgatttga ccacctgtgg caggaacatt ttgccttcgc caaacagatc gccgaccacg
    11521 ttcatgccgt ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg
    11581 gcttcttcag tatcctgctc gataaactcg gttatgcctt tgaccagcga gtattccaga
    11641 cgtttattca cgtcccacga gcgccactcc gcttgctggg cgttagcagt gtcgtccgct
    11701 ttactgccgc gatatttctc ggcaagctcc agtaaacgct cagtgccatc gtcacgacga
    11761 ttaagaatca catcttccac cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc
    11821 agttgcccgg cgttgacgat ccccatatcc atgccattgc gaatagcgta gtagaggaac
    11881 actgcgtgaa tggcttcgcg caccggatcg ttgccacgga atgagaaaga aacgttagat
    11941 acgccgccgg aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata
    12001 aagtcctgcg cgtagttgtt gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg
    12061 tcgaagatga tatcttctgg cgggaagcca acctcttcgg tgaggatttt gtacgcccga
    12121 cggcaaatct cgattttccg tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc
    12181 accaccactg ccgcaccgta gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg
    12241 ccctctttca tcgagataga gttaacaatg cctttgccct ggatacactt cagacctttt
    12301 tcaatgacat cccattttga ggagtcgatc ataatcggca cgcgagcgat atccggttca
    12361 ccggcaatca gattgagaaa acgcaccatc gccgcttcgg catcgagcat cccttcatcc
    12421 atgttgatat cgataatctg cgcgccgttt tccacctgct gacgcgccac atccagcgcc
    12481 tcgctgtatt tctcttcttt gatcaggcgc ttgaacttag cagagccggt gacgttggtg
    12541 cgttcaccca cgttcacaaa caggctatcg tcgccaatgt tcagcggctc caggccagac
    12601 aaacggcagg ctacgggaat ttccggcagt ttgcgcggcg ctaatccttc tactgcacga
    12661 ctcatcgctg caatatgttg cggcgtggtc ccgcagcaac caccgacgat attgagaaag
    12721 cccgcttgcg cccattcacg tatctgtttt gccatcgtgt cggcgtcgag atcgtattca
    12781 ccaaaggcgt taggcagccc ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt
    12841 gatagttcct gcacgtactg gcgcagttca tcgggcccca gcgcacagtt caggccaaag
    12901 gtcagcgctt cggcgtggcg caatgagttg taaaatgctt cggtggtctg cccggagagc
    12961 gtgcgcccgg aggcgtcggt gatggtgccg gagatcataa tcggcagctc aacgcccagc
    13021 gcttcaaact ccgttttcac cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg
    13081 gtttcaatca ggatcagatc cgcaccacct tccaccagcg ctttggtgga ttcgcgatag
    13141 gccgccgcca gctggtcaaa agtgatgttg cggaatgccg gatcgttgac gtccggagaa
    13201 atagacgccg tgcggttggt tgggccaaga acaccggcaa cgtagcgcgg tttctccggc
    13261 gtgcgcgcgg tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt
    13321 tccgccgaca gggattccat ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg
    13381 ttggtttcga tgatatccgc gcccgcttca aagtaggcgt tgtggatagc ggcgatcact
    13441 tccggtttac tgagtaccag caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca
    13501 aagcgttcac cacgaaaatc ggcttcgttc agtcgatagc tctggatcat ggtgcccata
    13561 ccgccgtcca acaccagaat acgttcattt aactgcgcac gcagttgttc cactttgctg
    13621 ctcacacttg ctcccgacac gcttaaccca gatttaataa aaattcaaca aaccatactg
    13681 gaataaacgc atctgtggta aaagcgacca ccacgcaaca tgagatttgt tcaacattaa
    13741 ctcatcggat cagttcagca actattgcat tagctaacaa taaaaatgaa aataatttcc
    13801 acgatacaga aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa
    13861 cccgccgttg ccaccgcacc agcgactgga caggttcagt ctttaacgcg tggcctgaaa
    13921 ttactggagt ggattgccga atccaatggc agcgtggcac tcacagagct ggcgcaacaa
    13981 gccgggttac ccaattccac gacccaccgc ctgctaacca cgatgcaaca gcagggtttc
    14041 gtgcgtcagg tcggcgaact gggacactgg gcaatcggcg cacatgcctt tatggtcggc
    14101 agcagctttc tccagagccg taatttgtta gcaattgttc accctatcct gcgcaatcta
    14161 atggaagagt ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg
    14221 attattatcg accaggtaca gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt
    14281 aaattgccga tgcacgcttc cggtgcgggt aaagcctttt tagcccaact gagcgaagaa
    14341 caggtgacga agctgctgca ccgcaaaggg ttacatgcct atacccacgc aactctggtg
    14401 tctcctgtgc atttaaaaga agatctcgcc caaacgcgca aacggggtta ttcatttgac
    14461 gatgaggaac atgcgctggg gctacgttgc cttgcagcgt gtattttcga tgagcaccgc
    14521 gaaccgtttg ccgcaatttc tatttccgga ccgatttcac gtattaccga tgatcgcgtg
    14581 accgagtttg gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc gtacggtgga
    14641 atgcgctgac tttttctggc gggcagaggc aatattctgc ccatcatacc tgagtggcaa
    14701 tagaataagg gtgtctgtta atcgcattga cgccaaaata acttaatgtc atacacttca
    14761 cacactttta ttaactcagc attattttta aacatcaaac cacttaatta ttaacaaaat
    14821 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
    14881 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
    14941 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
    15001 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
    15061 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
    15121 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
    15181 ttccacgtcc gtcttacaag ctgataagaa agaaattacg atgattaata aaaacgaaaa
    15241 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
    15301 tcaacgctca gacactcttg ataaacaaga attaaaccaa aaatcagata gaaaagaaaa
    15361 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
    15421 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
    15481 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
    15541 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
    15601 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
    15661 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
    15721 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
    15781 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
    15841 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
    15901 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
    15961 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
    16021 aatcaccaac ttacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
    16081 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
    16141 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
    16201 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
    16261 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
    16321 ttacctaaac tggccgcaac acatcattta gataatgaac aggtgtataa attcctgagt
    16381 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
    16441 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
    16501 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
    16561 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
    16621 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
    16681 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
    16741 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
    16801 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
    16861 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
    16921 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
    16981 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
    17041 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
    17101 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
    17161 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
    17221 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
    17281 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
    17341 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
    17401 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
    17461 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
    17521 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
    17581 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
    17641 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
    17701 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
    17761 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
    17821 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
    17881 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
    17941 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
    18001 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
    18061 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
    18121 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
    18181 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
    18241 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
    18301 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
    18361 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
    18421 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
    18481 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
    18541 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
    18601 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
    18661 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
    18721 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
    18781 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
    18841 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
    18901 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
    18961 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
    19021 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
    19081 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
    19141 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
    19201 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
    19261 tgttaaacca catgctgtgg atacctgcta gggtgatgaa ctggaacttg tagcccatat
    19321 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
    19381 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
    19441 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
    19501 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
    19561 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
    19621 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
    19681 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
    19741 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
    19801 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
    19861 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
    19921 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
    19981 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
    20041 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
    20101 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gttgttgatg tagccttttt
    20161 tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc cagttgcgcc agcgtgcatt
    20221 caggattgac tgaaccgcgt aatttcacca catcttccgc actgtatggg cgagtaatgc
    20281 cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt gtacgggttt
    20341 tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt agcctggcag
    20401 ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc gtgcggcatc
    20461 gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg ctggcaatga ctttcatctc
    20521 ttcgcccagc atctggcgga acaaggcttt ggtcaccggt ttgccattgc tcaacgtttt
    20581 ttgatgatgg atccactgcc agatcgaggt acgggaaatt tcagccgtcg ccgcatcttc
    20641 catcaggcca taaatcggca cgcagccgtt gccggagatc cacgcttcga tgtactgcac
    20701 agccacgcga atgttagcgc gcataccttc ttcggtgcgt tcaccatcac aaggtgccag
    20761 cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat acttcaagct gatttttacg
    20821 ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg ggtgagcgat
    20881 ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta tccgctttta ctttgttgag
    20941 cacctggtta ttacgctctt catctttgct cggaataaac gcggccatgc cgcccatcgc
    21001 aaaagcaccg cgtttatggc aggttttaat cagcaggcgt gagtaagcat tcaggaatgg
    21061 tttatccatc gtcacggcct gtctgtctgg caggacacga tcgggatagt ttttcaacgt
    21121 tttgatatag ctgaagatgt aatcccagcg accgcagttc agaccaacaa tatggtcgcg
    21181 cagcgcgtga aggatttcgt ccatctggaa cacggcgggc agcgtttcaa tcagcaacgt
    21241 cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga agacttcgct
    21301 ccaccaggcc gcttcctgct aggactgggt tttcggcaga tagaaatagg gaccgctgcc
    21361 ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa acaggctacc
    21421 ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac cgcgtacccg
    21481 acaaatcaaa accgctggat tgggcttgag ctgataaatt ttgcctgctt cattggtata
    21541 gctgatggta ccgttaaccg catcgcgcag gttaatttgc ccgtcgatca ctttgttcca
    21601 gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg cgttcagtgc
    21661 gttgatcacc atcttgcgct ctaccgggcc agttatctct acgcggcggt cttctaagtc
    21721 cgcaggaatc ccgcgaattt tccaatcagc atcgcgaatg gaagccgttt ccgaaataaa
    21781 atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg ccagaagttt
    21841 attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg cttcggcagt
    21901 aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa gccagttcat cggttgttgt
    21961 tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc ctctgtgaac
    22021 gaaggaacgt tgtgcgcttt tcgttcagca caactaagac tactcattta aaatgcaaaa
    22081 tcaaaaacaa tttccatttt aattaaaaat acttgataac ttattgataa caatatgaat
    22141 aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa aaggcacccg aaggtgcctg
    22201 aggtaaggtg ctgaatcgct taacgatcga ctatcacaga agattaatcc agcgttggat
    22261 tcatgtgccg tagatcgtat ggcgtgatct gatagacgta atagttgagc cagttggtaa
    22321 acagcaaatt accgtgacta cgccagctcg ctcgcggttt gttttgcgga tcgttgtgcg
    22381 ggaaatagtt atacggtaca tccgggtcta gtccggcttc cacatcgcgt aaatattcct
    22441 gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg cgcttatctt
    22501 tgctggcaaa cagatatgca tccccttctt ccgtctctgc cagaatttcc agatcggtgt
    22561 aatcacgaat caacgctgcc ggaaagtcag catagcgcga atgcggtgcc aggaatgaat
    22621 catcaaagcc acgcgtcaga agcgcatgag gatggagaat atgatgctcg taaacgccag
    22681 agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag gatattgagc gcggcctgta
    22741 ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc agcacctgtt
    22801 tgatctgcgg ccagtaagcg acatcattaa actccaccag gcccagcggc gcaccggtta
    22861 caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa gttacagtag aagttgttca
    22921 gatgctctgc gggcgtgttg cgcgattcac gggaatcgat gcgcaacagc tgaatatcga
    22981 cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt ttcagtttca atcttcttcg
    23041 gcatcaggtt aaggatcaga accttaagtg gacgaatttc ctgaccagac gcacgagaag
    23101 ttgtcatcac aaagacgttt tcttcacgca agaaattgac ggcgggtagc tcgtccggca
    23161 cacgaatcgg cataacctga ttacctcact acatacgctt atacgtttag acatccagat
    23221 agctgaagat aaccagaaaa tttgccaatg tcgagcctgc atgttgaagg tgagaaagtt
    23281 tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa ggagagaaaa tggttattag
    23341 tattcgccgc tcacggcatg aggaagggga ggaactcgtt gcgatttggt gtcgatctgt
    23401 cgatgccacc cacgattttt tatcagcaga gtatcgggcc gagctggaag agctggtgcg
    23461 ttcctttctg ccggaagcac cgttgtgggt cgcggttaat gaacgggagc agccggttgg
    23521 gtttatgttg ttaagtgggc agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg
    23581 ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg atggcaccgg aactgacaac
    23641 caacgttaat gagcaaaatg agcaggcggt tgggttctat aagaaggtgg gttttaaggt
    23701 tacgggacgc tctgaggtgg acgatttggg gaaaccgtat ccgttgctga atctggcgta
    23761 tgtgggggaa taagccgcct ctgccgtgcc tgcccaagct ggagaagacg cgccagcgtc
    23821 gcacccggca tttttttcac caacgcaaaa aggccatcct gacggatagc cttt
//
LOCUS       DAJTRW010000058        22925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRW010000058 DAJTRW010000000
VERSION     DAJTRW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="OO326_002499"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="OO326_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5966848.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1197..1859
                     /locus_tag="OO326_002500"
     CDS             1197..1859
                     /locus_tag="OO326_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5966849.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="OO326_002501"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="OO326_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5966850.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2747
                     /locus_tag="OO326_002502"
                     /pseudo
     CDS             2393..2747
                     /locus_tag="OO326_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2785..3090
                     /locus_tag="OO326_002503"
     CDS             2785..3090
                     /locus_tag="OO326_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5966851.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /locus_tag="OO326_002504"
     CDS             3093..3497
                     /locus_tag="OO326_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5966852.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /locus_tag="OO326_002505"
     CDS             3487..3786
                     /locus_tag="OO326_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5966853.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4268
                     /locus_tag="OO326_002506"
     CDS             3972..4268
                     /locus_tag="OO326_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5966854.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4338..5324
                     /gene="yqjG"
                     /locus_tag="OO326_002507"
     CDS             4338..5324
                     /gene="yqjG"
                     /locus_tag="OO326_002507"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5966855.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5617..5985
                     /locus_tag="OO326_002508"
     CDS             5617..5985
                     /locus_tag="OO326_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5966856.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6192..7088)
                     /gene="yhaJ"
                     /locus_tag="OO326_002509"
     CDS             complement(6192..7088)
                     /gene="yhaJ"
                     /locus_tag="OO326_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5966857.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7193..7894
                     /locus_tag="OO326_002510"
     CDS             7193..7894
                     /locus_tag="OO326_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5966858.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7917..8081
                     /locus_tag="OO326_002511"
     CDS             7917..8081
                     /locus_tag="OO326_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966859.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8293..9603)
                     /locus_tag="OO326_002512"
     CDS             complement(8293..9603)
                     /locus_tag="OO326_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5966860.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9631..10962)
                     /locus_tag="OO326_002513"
     CDS             complement(9631..10962)
                     /locus_tag="OO326_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5966861.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11237..12601)
                     /gene="tdcG"
                     /locus_tag="OO326_002514"
     CDS             complement(11237..12601)
                     /gene="tdcG"
                     /locus_tag="OO326_002514"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5966862.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12673..13062)
                     /locus_tag="OO326_002515"
     CDS             complement(12673..13062)
                     /locus_tag="OO326_002515"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5966863.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13076..15370)
                     /gene="tdcE"
                     /locus_tag="OO326_002516"
     CDS             complement(13076..15370)
                     /gene="tdcE"
                     /locus_tag="OO326_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5966864.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15404..16612)
                     /gene="tdcD"
                     /locus_tag="OO326_002517"
     CDS             complement(15404..16612)
                     /gene="tdcD"
                     /locus_tag="OO326_002517"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5966865.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16638..17969)
                     /gene="tdcC"
                     /locus_tag="OO326_002518"
     CDS             complement(16638..17969)
                     /gene="tdcC"
                     /locus_tag="OO326_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5966866.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17991..18980)
                     /gene="tdcB"
                     /locus_tag="OO326_002519"
     CDS             complement(17991..18980)
                     /gene="tdcB"
                     /locus_tag="OO326_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5966867.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19079..20017)
                     /gene="tdcA"
                     /locus_tag="OO326_002520"
     CDS             complement(19079..20017)
                     /gene="tdcA"
                     /locus_tag="OO326_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5966868.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20207..20551
                     /gene="tdcR"
                     /locus_tag="OO326_002521"
     CDS             20207..20551
                     /gene="tdcR"
                     /locus_tag="OO326_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5966869.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20807..21347
                     /locus_tag="OO326_002522"
                     /pseudo
     CDS             20807..21347
                     /locus_tag="OO326_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21369..21716
                     /locus_tag="OO326_002523"
     CDS             21369..21716
                     /locus_tag="OO326_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966870.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21885..22535
                     /locus_tag="OO326_002524"
     CDS             21885..22535
                     /locus_tag="OO326_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966871.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6182 a         5780 c         5286 g         5677 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgctgct
      121 gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact gcctgccgaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca taacccgtac
      601 tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg tgatgaccac
      661 gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg
      721 cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga tttcgagttc
      781 aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac ggccaccagc
      841 ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt ctcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcaatcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg
     1141 cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaatggct
     1321 tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg
     1381 cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg atgagcggtc
     1681 tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc
     1921 tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg
     2041 acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
     3001 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3421 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtaga
     3661 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3781 ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt ttgaatattt
     3841 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3901 gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac attttaatgc
     4021 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     4081 atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta tttcacagca
     4141 actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat
     4201 tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg ctgaataaaa
     4261 agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc
     4321 tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg
     4381 ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca
     4441 ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt
     4501 atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac
     4561 tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg
     4621 gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat
     4681 ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg
     4741 tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca
     4801 tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc
     4861 tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt
     4921 ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg
     4981 aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc
     5041 taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga
     5101 cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat ggcttcctgc
     5161 gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac atccgtaatc
     5221 attacttccg cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc
     5281 aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg
     5341 aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct
     5401 tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag gccagataag
     5461 cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc acggcacttt
     5521 attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc
     5581 tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta
     5641 aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg
     5701 gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc
     5761 gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt accgtggtgg
     5821 gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgctattc
     5881 ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca
     5941 ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt aagcaccttg
     6001 taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg cggcgtaaac
     6061 gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc gtaggcctga
     6121 taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg
     6181 tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca ccaggatttc
     6241 gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc
     6301 gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc
     6361 gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt
     6421 aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc
     6481 gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc cggctcctga
     6541 tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag cttgcgcgag
     6601 ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc cgcccgcccc
     6661 tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag ttgggtattg
     6721 gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg
     6781 gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt
     6841 ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc gacattggtg
     6901 aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc ttccagtttt
     6961 tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa
     7021 ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa tgccctttct
     7081 ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat ctggctaaca
     7141 atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa ttatgattac
     7201 tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg cccggtatac
     7261 tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct
     7321 taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc ccaaagtcga
     7381 tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag gcaatcatgt
     7441 tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca gctatagcga
     7501 acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg atacctgccc
     7561 gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc agcaattaat
     7621 cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt ggctgcacca
     7681 tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac gcgcgtattt
     7741 gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag cggcgctaac
     7801 ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg ccgattcccc
     7861 actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag atgatgatga
     7921 gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa gaacctgctc
     7981 gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct atcgtcgcgg
     8041 atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt gccggatgcg
     8101 gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa acaccccgta
     8161 ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca acaacatcaa
     8221 caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc
     8281 aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg
     8341 ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa catcatgcgc
     8401 cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca
     8461 cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat
     8521 catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc
     8581 atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag
     8641 tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag
     8701 cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat
     8761 tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc
     8821 gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt
     8881 cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt
     8941 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
     9001 gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
     9061 aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt gggtaaacac
     9121 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
     9181 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
     9241 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg taattgcctg
     9301 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
     9361 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
     9421 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
     9481 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
     9541 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
     9601 catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
     9661 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
     9721 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
     9781 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
     9841 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
     9901 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     9961 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
    10021 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
    10081 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
    10141 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
    10201 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
    10261 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
    10321 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
    10381 cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga ataacaattt
    10441 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
    10501 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
    10561 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
    10621 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
    10681 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
    10741 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
    10801 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
    10861 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
    10921 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt
    10981 ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg
    11041 caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc
    11101 aagactttaa aataacggag atggataaga attttctact aaattaatca caagagaata
    11161 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
    11221 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
    11281 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
    11341 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
    11401 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
    11461 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
    11521 tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg ccaatctccc
    11581 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
    11641 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
    11701 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
    11761 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
    11821 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
    11881 cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
    11941 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc
    12001 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
    12061 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
    12121 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
    12181 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
    12241 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
    12301 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
    12361 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
    12421 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
    12481 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
    12541 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
    12601 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
    12661 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
    12721 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
    12781 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
    12841 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
    12901 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
    12961 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
    13021 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
    13081 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
    13141 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
    13201 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
    13261 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
    13321 gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg
    13381 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
    13441 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
    13501 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
    13561 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
    13621 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
    13681 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
    13741 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
    13801 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
    13861 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
    13921 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
    13981 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
    14041 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
    14101 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
    14161 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
    14221 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
    14281 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
    14341 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
    14401 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
    14461 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
    14521 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
    14581 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
    14641 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
    14701 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
    14761 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
    14821 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
    14881 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
    14941 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
    15001 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
    15061 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
    15121 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
    15181 ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
    15241 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
    15301 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
    15361 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
    15421 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
    15481 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
    15541 tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
    15601 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
    15661 gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga acaaaggttt
    15721 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
    15781 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
    15841 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
    15901 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
    15961 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
    16021 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
    16081 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
    16141 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
    16201 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
    16261 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
    16321 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
    16381 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
    16441 ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
    16501 taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt acggaaaact
    16561 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
    16621 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
    16681 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
    16741 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
    16801 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
    16861 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
    16921 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
    16981 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
    17041 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
    17101 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
    17161 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
    17221 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag
    17281 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
    17341 gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
    17401 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
    17461 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
    17521 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
    17581 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
    17641 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
    17701 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
    17761 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
    17821 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta
    17881 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
    17941 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
    18001 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
    18061 acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
    18121 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
    18181 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
    18241 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
    18301 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
    18361 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
    18421 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
    18481 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
    18541 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
    18601 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
    18661 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
    18721 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa
    18781 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
    18841 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
    18901 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
    18961 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
    19021 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaacaact
    19081 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
    19141 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
    19201 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
    19261 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
    19321 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
    19381 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
    19441 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
    19501 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
    19561 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
    19621 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
    19681 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcttcag
    19741 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
    19801 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
    19861 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
    19921 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
    19981 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
    20041 ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt
    20101 aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa tatcaacaag
    20161 ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga cggggataac
    20221 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
    20281 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
    20341 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
    20401 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
    20461 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
    20521 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
    20581 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
    20641 agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc
    20701 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga
    20761 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
    20821 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
    20881 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
    20941 cgttaataaa atttacagta taagtaatga actccggaga gtattatctg caattactgc
    21001 attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa ccggaaaata
    21061 tgagtccatt catcatagat atttctacag gagaacatga tgattatatc atacaaacat
    21121 tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa
    21181 aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc acccaaaaag
    21241 aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca gattgttggc
    21301 aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    21361 ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc
    21421 gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag
    21481 gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac
    21541 caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg
    21601 taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac acccaaatta
    21661 ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa aactaggaaa
    21721 tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcagga
    21781 ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact
    21841 tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct aatactattc
    21901 taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt
    21961 ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg
    22021 ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt
    22081 taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa
    22141 aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat
    22201 ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag
    22261 aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat tatgaaaata
    22321 tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag
    22381 aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg
    22441 ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc aatattttac
    22501 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    22561 tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
    22621 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
    22681 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
    22741 cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
    22801 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatagtgtc
    22861 gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt
    22921 acgta
//
LOCUS       DAJTRW010000059        22306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRW010000059 DAJTRW010000000
VERSION     DAJTRW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..2579
                     /locus_tag="OO326_002525"
     CDS             <1..2579
                     /locus_tag="OO326_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5966872.1"
                     /translation="SLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFFKDDPSMVA
                     DLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVN
                     TDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERW
                     DEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQ
                     TAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGF
                     APVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSV
                     NSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGG
                     GQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLG
                     TNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQV
                     NISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPD
                     ADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDD
                     GNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVA
                     HSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALD
                     AASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDG
                     IQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAI
                     CR"
     gene            2570..3553
                     /locus_tag="OO326_002526"
     CDS             2570..3553
                     /locus_tag="OO326_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5966873.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3651..4191
                     /locus_tag="OO326_002527"
                     /pseudo
     CDS             3651..4191
                     /locus_tag="OO326_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4199..4714
                     /locus_tag="OO326_002528"
     CDS             4199..4714
                     /locus_tag="OO326_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5966874.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4680..5417
                     /locus_tag="OO326_002529"
     CDS             4680..5417
                     /locus_tag="OO326_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5966875.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5528..6538
                     /gene="pyrD"
                     /locus_tag="OO326_002530"
     CDS             5528..6538
                     /gene="pyrD"
                     /locus_tag="OO326_002530"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5966876.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6712..7254
                     /gene="zapC"
                     /locus_tag="OO326_002531"
     CDS             6712..7254
                     /gene="zapC"
                     /locus_tag="OO326_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5966877.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7251..8360)
                     /gene="ycbX"
                     /locus_tag="OO326_002532"
     CDS             complement(7251..8360)
                     /gene="ycbX"
                     /locus_tag="OO326_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5966878.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8604..10712
                     /gene="rlmKL"
                     /locus_tag="OO326_002533"
     CDS             8604..10712
                     /gene="rlmKL"
                     /locus_tag="OO326_002533"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5966879.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10724..12631
                     /locus_tag="OO326_002534"
     CDS             10724..12631
                     /locus_tag="OO326_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5966880.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12761..14014
                     /gene="pqiA"
                     /locus_tag="OO326_002535"
     CDS             12761..14014
                     /gene="pqiA"
                     /locus_tag="OO326_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5966881.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14019..15659
                     /gene="pqiB"
                     /locus_tag="OO326_002536"
     CDS             14019..15659
                     /gene="pqiB"
                     /locus_tag="OO326_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5966882.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15656..16219
                     /gene="pqiC"
                     /locus_tag="OO326_002537"
     CDS             15656..16219
                     /gene="pqiC"
                     /locus_tag="OO326_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5966883.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16475..16642
                     /gene="rmf"
                     /locus_tag="OO326_002538"
     CDS             16475..16642
                     /gene="rmf"
                     /locus_tag="OO326_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5966884.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16712..17230)
                     /gene="fabA"
                     /locus_tag="OO326_002539"
     CDS             complement(16712..17230)
                     /gene="fabA"
                     /locus_tag="OO326_002539"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5966885.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17299..19059)
                     /locus_tag="OO326_002540"
     CDS             complement(17299..19059)
                     /locus_tag="OO326_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5966886.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19244..19696
                     /gene="matP"
                     /locus_tag="OO326_002541"
     CDS             19244..19696
                     /gene="matP"
                     /locus_tag="OO326_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5966887.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19772..20818)
                     /gene="ompA"
                     /locus_tag="OO326_002542"
     CDS             complement(19772..20818)
                     /gene="ompA"
                     /locus_tag="OO326_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5966888.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21174..21683)
                     /gene="sulA"
                     /locus_tag="OO326_002543"
     CDS             complement(21174..21683)
                     /gene="sulA"
                     /locus_tag="OO326_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5966889.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21902..>22306
                     /gene="sxy"
                     /locus_tag="OO326_002544"
     CDS             21902..>22306
                     /gene="sxy"
                     /locus_tag="OO326_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5966890.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGE"
BASE COUNT         5686 a         5232 c         5848 g         5540 t
ORIGIN      
        1 acagcctgtt aagctctggt ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt
       61 cggcagcgtt caatgcacaa gctgaaacct ggttcgatcc tgcttttttc aaagatgatc
      121 cctcaatggt ggccgatttg tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt
      181 atcgtgtcga tattgttctg aatcagacaa ttgtagatac gcgcaacgtc aattttgttg
      241 agataacgcc agagaagggg attgccgcct gtttgacgac tgaaagcctg gatgcaatgg
      301 gtgttaatac tgatgcgttt ccggctttta aacaactgga caaacaagtg tgtgtgccat
      361 tggcggagat tattccggat gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa
      421 tttcagtacc gcaaatcgcc atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt
      481 gggatgaagg gatcaacgcg ctattactgg gatattcgtt tagcggggct aacagtattc
      541 atagcagcgc agacagtgat tctggcgaca gctattttct gaatttaaac agtggcgtta
      601 atttaggccc atggagatta cgcaacaatt caacatggag tcgcagtagt ggccaaaccg
      661 cagaatggaa gaatctcagc agctatttgc agcgggcggt tattccacta aaaggcgaac
      721 tgaccgtagg tgatgattat actgcaggcg attttttcga tagtgtcagc tttcgtggtg
      781 tgcagctggc gtcagatgac aacatgctgc cagacagcct gaaagggttt gcgcctgtgg
      841 tgcgtggtat cgccaaaagc aatgcccaga taacgattaa gcaaaatggt tacaccattt
      901 accaaactta tgtatcgcct ggtgcttttg aaattagtga tctctattcc acgtcgtcga
      961 gcggtgattt gttagttgaa atcaaagaag cggacggcag cgtcaatagc tacagcgtac
     1021 cgttttccag cgtgccatta ctccagcgtc aggggcgaat caaatacgcg gtgacactgg
     1081 cgaaatacag aaccaatagt aatgaacagc aggagagcaa atttgcccag accacgttgc
     1141 agtggggcgg accgtgggga acgacatggt atggtggtgg acaatatgct gaatattacc
     1201 gtgccgccat gtttggtctg ggatttaacc ttggcgattt cggagcaatt tcgttcgatg
     1261 cgacccaggc gaagagtacg ctggcagacc aaagcgaaca taaaggtcag tcatatcgtt
     1321 ttctgtatgc caaaacgctc aaccaattgg gcactaattt tcaattgatg ggctatcgct
     1381 attcgacgtc gggtttctac accctttccg acaccatgta taaacatatg gatggctacg
     1441 aatttaatga cggtgatgat gaagatacgc cgatgtggtc gcgttattac aatttgtttt
     1501 acaccaaacg tggcaaactg caggtcaata tctcccagca attaggcgag tacggttcgt
     1561 tttatttaag tggtagccag caaacttact ggcataccga tcaacaggat cggctattac
     1621 agtttggcta caacacgcaa attaaagatc tctcgctggg ggtttcctgg aactacagta
     1681 agtcccgtgg tcaacctgat gctgatcagg tgtttgcact aaatttttcc ctgccgctca
     1741 atctgttgct ccccagaagt aatgatagct ataccaggaa aaaaaattac gcctggatga
     1801 cctctaacac cagtatcgat aacgaagggc acattacaca aaacctgggt ttaacggaga
     1861 cactactcga tgacggtaac ctgagctaca gcgtgcaaca gggatataac agcgagggga
     1921 aaacggctaa tggtagcgcc agcatggact acaaaggggc gtttgcagat gcccgagtgg
     1981 gctacaacta cagcgataac ggcagtcaac aacaactgaa ctacgctctt tcaggcagtt
     2041 tagttgccca ttcacagggc attaccctgg ggcaatcgct gggggaaact aacgtgctga
     2101 ttgcagcacc aggcgcagag aatactcgtg tggcgaacag caccgggctg aaaactgact
     2161 ggcgcggata taccgttgtc ccctatgcca cttcttatcg ggaaaatcga atcgcacttg
     2221 atgcggcatc gttaaaacgt aacgtggatc ttgaaaatgc agtagtcaac gtggttccca
     2281 ccaaaggggc gttggttctg gcggagttca atgcccatgc gggagcaagg gtattaatga
     2341 aaacatcaaa gcagggtata ccgctgcgtt ttggcgcgat agcgacgctg gacggcatac
     2401 agactaatag cggcattatt gatgatgatg gctcgctcta tatgtccggt ttgcccgctc
     2461 agggagcgat aactgtacgc tggggcgaag ctcccgatca aatttgtcat atcagttacc
     2521 agcttaccga acaacaaatt aactctgcga ttacgcgaat ggatgccata tgcagataat
     2581 ctttggagaa aaatgcgtgt tattactacg actatttttt gccgccgtct taatgctatg
     2641 gtgcgctcaa actgctgctt atatcgggca gtgtcatacc actcagggga atccgtatat
     2701 tggcgtcaat tttggcgtta aaactctgga tgaagaagaa aatacggctg gagtagttaa
     2761 agacaaattt tatcagtgga acgaatcgaa tgattattat gtttcctgtg attgcgataa
     2821 agacaatgtc agaagtggcc gatgggcatt cgccgcggat tcaccgttag tctatttagg
     2881 cgacaactgg tacaaaatta atgactatct tgccgccaaa gttttattgc aggttaaagg
     2941 cagttctcct actgcggttc ctttcgaaaa cgtgggcaca ggggcagata cacgatggca
     3001 tatttgcgat cccggcggtc aacgtttagg tggccagggg gctagcggta atagcggtag
     3061 cttttccctg aaaatattgc agccgttcgt tggttcggtc gtcattcctc ctatggcgct
     3121 ggcgcgatta tttgaatgct acaacatacc cgcaggtgat tcctgcacga ctactggtac
     3181 accggtttta gtgtattacc tgtctggtac gatcaattca cttggctcat gttccgtcaa
     3241 tgccggagaa acaatcgagg ttgatctggg cgacgtattt gcggctaact ttcgtgttgt
     3301 agggcataag cctcttgggg ccagaacggc agaacttgca attccggtca ggtgtaacac
     3361 gggaaacgca gggttagtta acgtcaatct gagtctgacg gcaacctcag accccagcta
     3421 tccccaggcg attaagacgt cacgtcctgg cgtgggcgtg gtggtgaccg atagccagca
     3481 caacattatt tcccctgctg gtgggacact accgctctct attcctgatg atgcagacag
     3541 tatccgcgaa tgaatgtcta tccagtcagc acgacaagcg taccaccaga aaccgggcga
     3601 tttgaagcca cggcaacggt gagaataaat tttgattaac gcgtgaacgt atgaagaaaa
     3661 aaacgatata tcagtgcgtt attttgttct ttagccttct taacatccat gtcgggatgg
     3721 ctgggcctga acaagtcagt atgcatattt atgggaatgt ggtcgatcag ggctgtgatg
     3781 tcgccaccaa aagtgcatta caaaatattc atattggtga ttttaatatc agtgattttc
     3841 aggccgcgaa taccgtaagc actgctgctg atttgaatat tgatatcacc ggttgtgccg
     3901 ctggtattac tggcgcggac gtccttttta gcggcgaggc tgacaccctt gcgccgacac
     3961 tgctcaaact aactgacaca ggcggaagcg gtggtatggc aacggggatt gccgtgcatt
     4021 cttgatgcgc aaagtcagca agaaatcccg ctcaatcagg tccagcctct tacgccctta
     4081 aaagccgggg ataacacact caaatatcaa cttcgttata agtccacaaa ggcgggagca
     4141 acgggcggta atgcgacggc ggttctctat tttgatctgg tttaccagtg aaggagtgat
     4201 gttgaaacgg ataatctgga ttctgttctt actgggatta acggggggct gtgagctatt
     4261 tgcccatgat ggcacggtca acattagcgg atcgtttcgc cgtaatacat gcgtgctggc
     4321 acaggatagc aagcaaatca atgtgcagtt aggcgatgtc tcgctgacac gtttttttca
     4381 tgggaattat ggcccggaga aatctttcat cattaatttg caggattgcg gaacagatgt
     4441 cagcacggtt gatgtcacct tttcaggaac ccccgacgga gtgcagagcg aaatgctgtc
     4501 cattgaaagt ggtacggatg cagccagtgg gctggcaatt gcgattctgg atgatgcgaa
     4561 gatattgatt ccgcttaacc aggcgagtaa agactacagc ctacatagcg gtaaggtgcc
     4621 attaactttt tatgcgcaat tgcgacctgt caatagcgat gtgcagtcgg gtaaagtgaa
     4681 tgccagcgcg acatttgtac ttcattatga ctaatacgtg gaacagatta gcgctcttga
     4741 ttttcgccgt tttatcgctg ctggtggcgg gtgaattgca ggcaggagtg gtggtcggcg
     4801 gaacgcgatt tatctttccg gcagacagag aatcgatatc tattttactg actaatacca
     4861 gtcaggaatc ttggcttatt aacagcaaaa tcaatcgccc aacgcgttgg gccggggggg
     4921 aagcgtcgac agtgccagca ctattactgg ccgctccgcc acttattctc ctgaagcccg
     4981 gtactacagg cacgttgcgc ttgctgagaa cggaaagcga catcttgcct gtggatcgcg
     5041 aaacgctatt tgagttaagc attgccagcg tgccatccgg caaagttgaa aatcagagcg
     5101 taaaagtggc gatgcgctcg gtatttaaac tgttctggcg acccgaaggt ttgccgggcg
     5161 acccgctgga agcttatcaa caattacgct ggacacggaa ttcgcagggt gtacaactca
     5221 ctaacccaac gccttactac attaacctga ttcaggtgag cgtaaatggt aaagcgttaa
     5281 gtaatgcggg agtcgtgccg cctaaaagcc agcgtcagac aaactggtgt caggtaatag
     5341 caccgtgtca tgtcgcctgg cggacgataa atgattacgg cgggttgagt gcaaagaagg
     5401 agcaaaatct gccctgaaac aggttctgaa aacgtttgcg ttttttttgc cgcaggtcaa
     5461 ttcccttttg gtccgaactc gcacataata cgcccccggt ttgcacaccg ggaatccagg
     5521 agagttcatg tactacccct tcgttcgtaa agcccttttc cagctcgatc cagagcgcgc
     5581 tcatgagttt acttttcagc aattacgccg cattaccgga acaccgtttg aagcactggt
     5641 gcggcagaaa gtgcctgcga aacctgttaa ctgcatgggc ctgacgttta aaaatccgct
     5701 tggtctggca gccggtcttg ataaagacgg ggagtgcatt gatgcgttag gcgcgatggg
     5761 atttggatcg atcgagatcg gtaccgtcac gccacgtcca cagcctggta atgacaagcc
     5821 gcgtctcttt cgtctggtag atgccgaagg tttgatcaac cgtatgggct ttaataatct
     5881 tggcgttgat aacctcgtag agaacgtaaa aaaggcccat tatgacggcg tcttgggtat
     5941 taatatcggc aaaaataaag atacgccagt ggagcagggc aaagatgact atctgatttg
     6001 tatggaaaaa atctatgcct atgcgggata tatcgccatc aatatttcat cgccgaatac
     6061 cccaggatta cgcacgctgc aatatggtga agcgctggat gatctcttaa ccgcgattaa
     6121 aaataagcaa aatgatttgc aagcgatgca ccataaatat gtgccgatcg cagtgaagat
     6181 cgcgccggat ctttctgaag acgaattgat ccaggttgcc gatagtttag ttcgccataa
     6241 tattgatggc gttattgcaa ccaataccac tctcgatcgt tctctggttc agggaatgaa
     6301 aaattgcgat caaaccggtg gcttaagtgg tcgtccgctt cagttaaaaa gcacagaaat
     6361 tattcgccgc ttgtcactag aattaaacgg tcgcttaccg atcatcggtg ttggcggcat
     6421 cgactcggtt atcgctgcgc gtgaaaagat tgctgcgggt gcctcactgg tgcaaattta
     6481 ttctggtttt atttttaaag gtccgccgct gattaaagaa atcgttaccc atatctaatt
     6541 atttcttcga attcctcttt ataagataat cagggcttta ttttcagacc tggttgtttt
     6601 atattcatcg ttgttgctta tttagacatt ttgtactttt attgttgagg ttattaagcg
     6661 aagcgacaat ggattgtgtt gctgcggttt tatagggtag gggagaggca gatgcgaatt
     6721 aaaccagacg ataactggcg ttggtattac gatgaagagc acgatcgaat gatgctcgat
     6781 ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca aaatgttgac tccagatgcc
     6841 ttttctcccg caggcttttg tgttgatgat gccgcgctct atttctcttt tgaagaaaag
     6901 tgccgcgatt ttaatttatc taaagaccaa aaagccgaac tggtgctgaa tgcactggta
     6961 gcaattcgtt atctcaaacc gcaaatgccg aaaagctggc attttgtctc ccatggtgaa
     7021 atgtgggtgc ccatgccggg cgatgcggcc tgcgtttggt tgagcgatac tcacgagcag
     7081 gtcaatttgc tggtcgttga atctggcgaa aatgccgcgc tatgcctgct ggcacaaccc
     7141 tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat catgaacgac
     7201 aggctgaaac cgcaggtgaa tgttgacagc ttcagcctcg aacaggcagt ctaacgcgtc
     7261 agcttaaggg cagtcttcgg tacacagcta cagcaaagaa tggtgccatc atcgcccatt
     7321 gctgattttt tcagcggtgt gacttcgcct tctaaaagct gaacacggca acttccacaa
     7381 atgcccgcgc ggcaagaata agggatacga attccctgat tttctaattg ctccagcaac
     7441 acttgttggt tatttccacg aaatgcctgt ccctgccagt caatatctac atttgcgtcc
     7501 ggttgttgcg tgatgttggc ggtatcatca gcggcagctg cgccgtaaat ttttgccgga
     7561 gccgttgcca gaatttccac ctcatcgcca acgcgaatca cgccgctatt acgggcaatt
     7621 aaattctgac caaaatcgac atcgccgtta tcctgggcag tgcggaaaga ttgcaatgtt
     7681 tttaatggtt cgcctgccgg atgtttttgc cctttttctg ggctgacggt ggtgaaaata
     7741 cagcggctac aaggtttaac cacatcaaac accacatcac caatgcgaat cactttccag
     7801 ctatcttctt cccaggctga cgcgccggaa accaccagat tggggcggaa ttgctccatt
     7861 tttacactgg ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt agcaagaagg
     7921 taaggatagc catcagcaaa tgacagaggt acagtgttgt ggcgtttcac gcgccgggtc
     7981 atttgtggcc ccacccagcg taattgcact tcgcgggaga aaaatccact tagccatttg
     8041 ttgatcgcgt ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc ggttggtgcg
     8101 tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac tgccatctgg tgcggtgaga
     8161 tgcaagccat catgcacagg tgaaggagta aaccgtacca tctggggaaa ctggcgagcg
     8221 gtaataaacg taccgtcagg ttccgtgatc ataaagatgc gatcgaaggc cagaccactg
     8281 acatcagcca gagcatgtgt aagaccaatg ccgcgcatcg atttaacagg atgaataaaa
     8341 agccgggtta atgtcgccac ggtgcggtcc tcaaatgaaa ataagccctc aactttatga
     8401 catgacgcgc ttattagcta taatgcgcaa caattttctt agcgcctgtc ccgttaggct
     8461 attttatttg tcagtttggc cctgggttgt gctcgaaatc ctcacgtact tgtgtacgct
     8521 ccggtttctc cgcgctctcc atgtccagac tgactgcaac aattacgcct actgcggtag
     8581 gctcttagag taaaagtgac gatatgaatt ctctgtttgc cagtacggcc cgtgggctgg
     8641 aagagctgtt aaaaactgaa ctggaaaatc tgggggccgt tgaatgccag gtggttcagg
     8701 gtggggtcca tttcaagggc gacacacggc ttgtttacca gagcctgatg tggagccgcc
     8761 tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt ttacagcgat ttagacctct
     8821 atctcggtgt tcaggcgatc aactggacag agatgtttaa tcctggcgcg accttcgctg
     8881 tccacttcag tggtttgaat gacaccatcc gcaacagtca gtacggcgcg atgaaagtga
     8941 aagacgcgat cgtcgatgct ttcacacgga aaaatctgcc gcgtccaaat gttgatcgcg
     9001 atgcgccgga tatccgcgtt aacgtctggc tgcataaaga aaccgccagt atcgctcttg
     9061 atctgagtgg tgatggttta catctgcgtg gctatcgcga tcgtgctggt attgcgccga
     9121 tcaaagaaac cctggcagcc gctattgtga tgcgatccgg ctggcagtca ggaacaccgc
     9181 tgctcgatcc gatgtgtggt tccggtacgt tgctgattga agcggcgatg ctggcgaccg
     9241 atcgcgcacc aggcttgcac cgtgggcgtt ggggctttag cggctgggcg cagcatgatg
     9301 aagctatctg gcaggaagtg aaagcggaag cgcaaactcg cgcccgtaaa ggcctagctg
     9361 agtatagctc tcatttttac ggttcggaca gcgacgcacg ggtgattcaa cgtgcgcgca
     9421 ctaacgcccg tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa gatgtcgcgc
     9481 aactgaccaa tccgctgccg aaagggccgt acggtacagt gttgagcaac ccgccatacg
     9541 gtgaacgtct ggacagcgaa ccggcgctga ttgcgctgca tagtctgctg ggccgaatca
     9601 tgaaaaacca gttcggtggc tggaatctct ctttgtttag tgcctcgccg gatctgctaa
     9661 gctgcctgca gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg ctggactgcg
     9721 tacagaaaaa ttaccacgtt gccgaaagca caccagacag taaaccggca atggtagcgg
     9781 aagattacgc caaccgcctg cgtaagaacc tcaaaaaatt cgagaagtgg gcacgccagg
     9841 aagggattga atgttaccgc ctgtatgacg ccgatctgcc ggaatataac gttgccgttg
     9901 accgttatgc cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact attgatgcgc
     9961 acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac catttcggta ctggggattg
    10021 cgccaaacaa actggtgctg aaaacccgtg aacgccataa gggcaaaaac cagtaccaga
    10081 aactgggcga gaagggcgag tttcttgaag ttaccgaata taacgctcac ttgtgggtga
    10141 acctgacgga ttatctcgat actggtctgt tcctcgatca ccgtatcgcc cgccgtatgc
    10201 tcggtcagat gagcaaaggc aaagatttcc tcaacctgtt ttcttatacc ggcagcgcca
    10261 ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac cgtggatatg tcgcgtactt
    10321 atctggaatg ggcagaacgc aacctgcgtc tgaatggcct gactaggcgt gcgcatcgcc
    10381 tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttc gatctgatct
    10441 ttatcgatcc gccaaccttc tctaactcaa aacgaatgga agatgcgttt gatgttcagc
    10501 gcgatcatct ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt gggacgatca
    10561 tgttctcgaa caacaaacgt ggcttccgta tggatctcga cggcctggca aaactgggac
    10621 tgaaagcaca agaaattacg caaaaaacgc tctcccagga tttcgcccgt aaccgccaga
    10681 tccacaactg ctggctgatt accgcagcct gaaaggaata gtaatgtcat taatcagtat
    10741 gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc gataacgcag aactgcatat
    10801 cgaagataac gaacgtgttt gtctggtggg ccgcaacggc gcaggaaaat cgacgttaat
    10861 gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc attatttatg agcaagattt
    10921 gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt gagggtagcg tttatgattt
    10981 cgttgccgaa ggcattgaag aacaagcgga atatctgaaa cgctatcacg atatttcgcg
    11041 cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg ttcaggaaca
    11101 gctggatcac cacaacctgt ggcagctgga aaaccgcatc aacgaagtgc tggcgcaact
    11161 ggggttagat cctaacgttg cgctgtcgtc gctttccggc ggctggttgc gtaaagcggc
    11221 attaggacgc gcgctggtga gcaatccgcg cgtattgctg ctggacgaac cgactaacca
    11281 tctggatatc gaaaccatcg actggctgga ggggttcctt aaaaccttca atgggacgat
    11341 tattttcatc tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca ttgttgatct
    11401 cgatcgcggc aagctggtga cctatccagg gaattacgac cagtacctgc aggaaaaaga
    11461 agaagccctg cgcgtggaag aactacaaaa tgccgagttc gatctcaaac tggcgcagga
    11521 agaagtgtgg attcgccagg ggatcaaagc acgccgtacc cgtaatgaag gccgcgtacg
    11581 cgccctgaaa gcgatgcgtc gcgaacgtgg tgaacgtcgc gaagtgatgg gtaccgcaaa
    11641 gatgcaggtg gaagaggcca gccgctccgg taagatcgtt ttcgaaatgg aagacgtttg
    11701 ctaccaggtt aacggtaagc aactggtgaa agatttttct gcccaggttc tacgtggcga
    11761 caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc acgctgctga aactgatgct
    11821 cggtcagctt caagcggaca gcgggcgtat tcacgttggc accaaactgg aagtggctta
    11881 tttcgatcag caccgcgcgg aactggatcc cgataaaacg gtgatggata accttgccga
    11941 aggtaagcaa gaggtgatgg ttaatggcaa gccacgccac gtattgggct atttgcagga
    12001 ctttctgttc catccgaaac gggcgatgac gccagtacgt gcgttttctg gcggtgagcg
    12061 gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc aacttattga ttcttgacga
    12121 accgaccaac gatcttgatg tcgaaacgct ggaactgctg gaagaactga tcgacagcta
    12181 tcagggcacg gtattgctgg ttagccacga tcgtcagttt gtcgataaca ccgttacaga
    12241 atgctggatc ttcgaaggcg gcggtaaaat tggtcgttat gttggcggtt atcatgatgc
    12301 ccgtggtcag caagaacaat atgtggcact caaacagcct gcggtgaaaa aaagcgaaga
    12361 agccgccgcg ccaaaagcag aaactgtaaa acgcagcagt agcaaactaa gctataaatt
    12421 gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat ctggaggcga agctggaagc
    12481 cctacagacg caagtggcgg atgcttcctt cttcagtcag ccgcatgagc agacgcaaaa
    12541 agtgcttgct gatatggctg ctgcagagca ggagctggag caagcctttg aacgctggga
    12601 gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat agtcgattta aacagagaca
    12661 gctgacaacg gtaaaattgc ctgatgaact gggtttatca ggcatgaaaa ccaacacttt
    12721 gttagtcatc tgtaaaatag cgcatcatta aggagtacca atgtgcgaac atcatcatgc
    12781 cgcgaagcac atcctgtgct cgcagtgtga catgctggtg gcgttaccgc gccttgagca
    12841 tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt gggatgcccc
    12901 tcggcagcgt cccaccgcct atgcgttggc tgcactgttc atgctgttgc tgtccaactt
    12961 gtttcctttt gtgaatatga acgttgcagg agttaccagt gaaattacat tactggaaat
    13021 tcccggcgtg cttttttctg aggactacgc cagcctcggc acctttttcc tgttgtttgt
    13081 gcaactggtt cccgcgtttt gtctgataac cattctgtta ctggtgaatc gcgcggaatt
    13141 accggtccgt ttaaaagagc aactggcacg ggtgcttttt caactcaaaa cctggggaat
    13201 ggcggagatt ttcctcgcgg gtgtgctggt cagtttcgtt aaactgatgg cttacggcag
    13261 cattggggta ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc aactgcgcgc
    13321 ttttcagtgc gttgatcgtc gctggttatg ggacgacatc gccccgatgc cagaactgcg
    13381 ccagccgcta aaaccaggcg tcacggggat acgtcagggg ctgcgttcgt gctcctgttg
    13441 tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt tgtagtacca aagggtacgt
    13501 tcggcgtaga aacagcctgc agtggacact cgcgctgctt gtaacgtcca tcatgctgta
    13561 tcttccggct aatattttgc ccatcatggt gacggattta ttaggctcga agatgccgtc
    13621 gacgattctc gctggggtca ttctgttatg gagcgaaggc tcttatcccg tcgctgcggt
    13681 gatctttctg gccagtatta tggtgccaac gttaaagatg atcgccatcg cgtggctgtg
    13741 ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga atgcatttga tttatgaagt
    13801 tgttgagttt gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg tgctctcggc
    13861 gctggtgcgt atgggaggtt taatgagtat ttatccggca atgggtgcat taatgtttgc
    13921 tttagtcgtc ataatgacaa tgttttctgc tatgacgttt gacccgcgtt tgtcgtggga
    13981 tcgtcaacct gaatcagagc atgaggagtc ctgacagtat ggaatctaat aatggggaag
    14041 ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat atttcctatc gtcacggcgc
    14101 tcattggggc ctgggttctt ttttatcatt acagccatca gggaccggaa gtgaccctga
    14161 tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac cattaaaagc cgtagcgttg
    14221 acgtcggcgt ggttgaaagc gccacactgg ctgatgattt gacgcacgtt gaaatcaaag
    14281 cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga caccgtcttt tgggtggtga
    14341 aaccgcagat tggtcgcgaa gggattagcg gcctgggaac gctgctgtct ggagtttata
    14401 tcgaactgca gccaggcgcg aaaggcagca aaatggataa atacgatttg ctggactcac
    14461 caccgttggc cccgcctgat gcgaaaggta tccgtgtgat tctcgatagc aaaaaagccg
    14521 ggcagctctc gccaggagat ccggtgctgt tccgtggcta tcgggtaggt tcggttgaaa
    14581 ccagcacctt cgatacgcaa aaacgcaata tcagttatca actgttcatc aatgcacctt
    14641 atgaccgact ggtgaccagc aatgttcgct tctggaaaga tagtggcatt gcggttgatc
    14701 tgacgtcagc ggggatgcgt gtggagatgg gctcattgac aacgctgctt agtggcggtg
    14761 ttagctttga tgtgccggaa ggtctggatt tagggcagcc agtggcaccg aaaacagctt
    14821 tcgttttgta tgatgatcag aagagcattc aggattcgtt gtacaccgat cacattgatt
    14881 atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc gggagctccg gtagaattcc
    14941 ggggtattcg cctgggtacc gtaagcaaag tgccattctt tgcgccgaat atgcgtcaga
    15001 catttaacga tgattaccgt attccggtac tgattcgtat cgagccagag cggctgaaaa
    15061 tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg aaacgtggtt
    15121 tacgcggatc gctgaaaacc ggaaacctgg tcactggtgc actgtatgtt gatctcgatt
    15181 tctatccaaa tacgcctgca ataaccggta ttcgtgaatt taatggttat cagattatcc
    15241 cgactgttag cggcggcttg gcgcaaatcc agcaacgact gatggaagcg ttggataaga
    15301 tcaacaaact gccattgaat ccgatgattg aacaggcaac cagtacgctt tctgaaagtc
    15361 agcgcacaat gaaaaacctg caaacgacgc tggatagcat gaacaagatc ctcgccagcc
    15421 agtcgatgca gcagttgccg acggatatgc agtcaacgtt gcgtgaattg aatcgcagca
    15481 tgcagggctt ccagcctggc tccgcagcct acaacaagat ggtggcggat atgcagcgcc
    15541 ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac gctcaatgag aagagtaacg
    15601 cgctggtatt tgaagcgaag gacaaaaaag atccagagcc gaagagggcg aaacaatgaa
    15661 aaagtggcta gtgacgattg cagcactgtg gctggccgga tgcagctccg gcgaaattaa
    15721 taaaaactat taccagttac ctgtggtgca gagcggtaca caaagtaccg ctagccaggg
    15781 caatcgtctg ttatgggtag agcaggtcac tgttcctgac tatctggcgg ggaatggtgt
    15841 ggtttatcaa accagtgatg tgaagtatgt gattgccaac aacaacttgt gggccagccc
    15901 gttggatcaa cagttgcgca acaccctggt tgccaacctg agtacgcaac tgcccggctg
    15961 ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta ccgtaacgga
    16021 gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt tgaaccacca
    16081 gggacaactg atcaaacgtc cgttccgtct ggaaggagtg caaactcagg atggttacga
    16141 tgagatggtt aaagtgctgg ccggtgtctg gagtcaggaa gccgcttcta ttgcacaaga
    16201 gataaagcgt ctaccttaat tataaagatt tgtaaatata accgtctccg gtatgttgcc
    16261 tgaggcggtt tttttgtttc taacgtgcgg aaaaatttgt tcctcttcac attttttgta
    16321 caaccgacat gcccgtgtag ctcacaaata tgacagtggc gtgaattttg cgcattgacg
    16381 gcagttatga ttcgcggtat tgcttaactg tgattgcaca tttagtaatc actgttttct
    16441 tttccaccag aaaccagtat gagggaaacg aggcatgaag agacaaaaac gagatcgcct
    16501 ggaacgggca catcaacgtg gttatcaggc cggcatcgcc ggacgctcaa aagaaatgtg
    16561 tccctatcag acgctgagtc aaaggtcaca atggctggga ggctggcgag aagccatggc
    16621 ggacagggta gtaatggcct gattctgtct ctttaaaaag aaacctccgc attgcggagg
    16681 tttcgccttt tgatactctg tctgatgata atcagaaggc agacgtatcc tggaacagac
    16741 cgactttcag gtcgctggcg gtatagatca gacgacaatc aaccagcact tcgccatctg
    16801 ccagacccat aatcagacga cgattaacaa tgcgtttaaa gtgaatacgg taggtcactt
    16861 ttttcgctgt cggcagtacc tgaccagtga atttcacttc gccaacgccc agcgcgcggc
    16921 ctttaccttc gccgcccagc cagccgaggt agaaccctac cagctgccac attgcgtcca
    16981 ggcccaggca tcccggcata accggatcgc caatgaagtg gcatccgaag aaccacagat
    17041 ccggattgat atccagttct gcttcaacat accctttgtc gaagttacca cccgtttcgg
    17101 tcattttgac cacacggtct atcatcagca tgttcggtgc tggcaattgc gggcctttag
    17161 cgccaaacag ttcaccgtga ccagaggcaa gaaggtcttc ttttgtatag gattcgcgtt
    17221 tatctaccat gttctctgta agccttattt tattgaagca cgcaggatag ctaacacgtg
    17281 tacgctgaac aagtccgatc agttcggaat aaaccagttc agccaacgta atggccatgg
    17341 aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata cgttcctgga tggtttgcat
    17401 cagcgtcgtt tggccttcgc catcccacac cagatttaat aataacggca gtgcgtcagt
    17461 cacatcgtct actgcccaga tggtgaattt gccttcttct accgctttca ccagttcact
    17521 gtgaagactt aaatggcgaa cgttagcagt ggggataatg acaccttgtt tccccgttaa
    17581 ctcacgttgc tggcaaatag caaagaagcc ttcgattttc tcatttaaac caccgaccgg
    17641 ctggacgcgg ccgaactgat cgactgaacc tgtgatagcg atactctgat tcactggcac
    17701 atcagcgagg gcgcttatca gggcgcagag ttcagccatc gaggcactat cgccatccac
    17761 ttcactgtat gactgctcaa atgtcagcga tgctgagaag gggatctgtt gctcaagctg
    17821 tagttccgac atcaggaacg cctgcatgat catcatccct ttcgcatgga tattgccgcc
    17881 aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca caacgcagct
    17941 aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga aattcaatga ccgaaagggc
    18001 gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct gaaggatctc
    18061 atcctgcata cgttcagcga ggaaaccttc gcgccattca cgctgctgca gcattaagtt
    18121 tagctgctcg ccggagaagg tgtcgccatc acacagggag gcgacctctt tacactggcg
    18181 gaggatccac tgcgggctaa gcggaagtgt ttcttgttca ccggtgtagc gtgctgcttc
    18241 gcggataagt accggccaag catccgctcc cggggcaggt aagtgattat gtctggcggt
    18301 aaatgtcacc cagcgacacc actggcttac tgactccgca tcgacaatct gcagagtatc
    18361 ttcaaattcg ctataaatag cctgctctga aagctctggc tccatctcct ggaaatcagc
    18421 caatgattcg cgttcgccta ccagaatgac tttcagcttc aatggcattg aaggcacaga
    18481 gacggggaga gggcgcgact catcaaacgc aacccagtca aaacgctcgc ggttaacgat
    18541 atttttcagc cgcatccaca gcagaggttg cgccagcagt gtacgcaaag agatgatgag
    18601 aataccgcca tttgcctgat gcaccagacc aggctgcagg gtaatgtcgc cattaaactg
    18661 gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg gcagcgacaa cttgcgttaa
    18721 agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag cggatggagt ggccggaaac
    18781 ctcatagtga tcgcccacca gttggcctgc atcgctttgt agcgtacgcg cggcatcggc
    18841 aataagattc agatactcag actcttccgg ggccttagcc agcataaaag aggaggatgc
    18901 tcgcgtatgc agcaactgct ccagcgcaaa ttgcagtcgc ggttgagtat cactgaataa
    18961 aggatcgttt tcgtcaatca aatgtggctg agcaaatatt tcctgatagc tatcggtatc
    19021 aggaaccagg tcacgccatg caagtttcgt aatggtcaaa gttgatgttt tttagtctgt
    19081 tgtcaaagcc gcgattatac cgtaaccggc actacagcac acgtagaaag caccgacaat
    19141 actcctggct gggcgttaaa gctcacagga tggagattct tttcttcact ggcctaaaaa
    19201 gctgatattc tgtaaagagt tacacggtaa cattgagatc gctatgaaat atcaacaact
    19261 tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg aagaagcatc gcgaagggga
    19321 gttaattacc cgttacatag aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc
    19381 gctcgaaaat gaacccgtgc tggtaaatgg ctggattgac aagcatatga atccggaact
    19441 ggtcaatcgc atgaagcaga ccattcgggc cagacggaag cgccatttta atgcggaaca
    19501 ccagcatacg cgcaaaaaat cgatcgatct ggaatttatt gtctggcaac gtctggctgg
    19561 tcttgcacag cgtcgcggta aaacgctttc tgaaacgatt gttcagctga ttgaagatgc
    19621 ggaaaacaaa gagaaatacg cgaataaaat gtcttctctt aagcaggatc tgcaggcatt
    19681 gctgggtaag gaataactga cgaaagtcag ttcaatttac taaaggcaaa aaaaaccccg
    19741 cagcagcggg gtttttctac cagacgataa cttaagcctg cggctgagtt acaacgtctt
    19801 tgataccttt aacttcgatc tctacgcgac gatccggagc caagcagtcg atcagtgcag
    19861 cacgctgttt cacgttgtca caggtgttgc cagtaaccgg gttggattcg cccataccac
    19921 gtgcggagat cttgtctgcc gggatacctt tggagatcag gtaatcaaca acagactgag
    19981 cacggcgctc ggacagaccc tggttgtaag cgtcagaacc gatgcggtca gtgtaaccca
    20041 gaacaactac ggaaccgtct ttcggatcta ggttgctcag ctggctgtac agctgatcca
    20101 gagcagcctg accttccggt ttcagggttg ctttgttgaa gttgaacaga acgtcagact
    20161 tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc tggagcaact accggagctg
    20221 cttcgccctg accgaaacgg taggaaacac ccaggctcag cagaccgttg tccggacgag
    20281 taccgatggt gtttgcgtca ccgatgttgt tggtccactg gtattccaga cgggtagcga
    20341 tttcaggagt gatcgcgtac tcaacaccac ctgcgaaaac cggagaaacg ccagtgtcgt
    20401 ggtctttaaa ggatgcgcca ccaggtacgt tagccttggt gtctgcacgc cataccatac
    20461 cacccagacg agtgtagatg tccagatcgt cagtgattgg gtaacccagt ttagcggtca
    20521 gctgaacgcc ctgagctttg tatgcgccgt tgatgttgtc gcctttgtac ggcatacgac
    20581 ctaaccagtc gtaacccatt tcaaaaccaa catacgggtt aacctggtaa ccaccaaaag
    20641 cacctgcacc cagttggttt tcgtgggtcg gaccattgtt aggaataaaa ccagtgtcat
    20701 ggtactggga ccagcccagt ttagcaccag tgtaccaggt gttatctttc ggagcggcct
    20761 gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc gatagctgtc tttttcattt
    20821 tttgcgcctc gttatcatcc aaaatacgcc atgaatatct ccaacgagat aacacggtta
    20881 aatccttcac cgggggatct gctcaatatt aactctaccg atatcttcgg cttatgccga
    20941 gcacccctgg cgatgtaaag tctacaacgt agttgaaaac ttacaagtgt gaactccgtc
    21001 aggcatatga aaaaaaagtc ttgtataagg tatgtttaat ctttttgtca gcgacaattt
    21061 acagaaaaaa aacgcggaaa ccgcttcaga caagccttcg caaggaaaat tagtcacgac
    21121 tgaaagcatt ggctgggcga caaaaaaagt tctaggatta atcctaaatt tacttaatga
    21181 tacaaattag agtgaatttt tagcccggaa agttgtctcg ttgtgtgaga ggatgcgctt
    21241 accggacgca taataaaccc catagcgtta ccttcatttg ccgcatcaac aagttcagca
    21301 tgctcttctt cagtcaaatc atctgtcaac caaccgatca ccacactgta attgcccgtg
    21361 cgtaaagcgc gaaccattga ctccactgtg tggcaagggg agagctggct aatctgcatt
    21421 actttcgtta agggtagccc agatgcctga acccattccc gactcagttt ttgttgcggt
    21481 gttaaccaga gttgccagcg cgattgctga ccgagttgct gtaacaatgg caacagtaga
    21541 agttgcgtca tcatgggctg atcttcgcga tagacaactt cactgataag cccggctgta
    21601 gtgttttccg tagagacacg cgcaatttta cttgctgcgg atgagaacga cgaagaacga
    21661 tgtgcatagc ctgaagtgta cataatcaat ccagcccctg tgagttactg tatggatgtg
    21721 cagtacatcc agtgacaaca aagatcaacc ctattttcgg aaagagcctc gcaaattttg
    21781 tcgttggtga cgggaaaaca taaattaatc ttgcccctta agaataagtt gcctattttc
    21841 gtagttaacg gatccgttaa tgtgaatcat tcttttatgt tatgatttta aaaggaattt
    21901 tatgaaaagc ctctcctata agcggatcta taaatcacaa gaatacctgg caacgttggg
    21961 cacaattgaa taccgatcat tgtttggcag ttacagcctg accgttgacg acacggtgtt
    22021 tgcgatggtt tctgatggtg agttgtatct tcgggcttgt gagcaaagtg cacagtactg
    22081 tgtaaaacat ccgcctgtct ggctgacata taaaaagtgt ggccgatccg ttaccctcaa
    22141 ttactatcgg gttgatgaaa gtctatggcg aaatcaactg aagctggtgc gtctgtcgaa
    22201 atattctctc gatgcagcgc tgaaagagaa aagcacgcgc aatacccggg aaagactgaa
    22261 agatttgccc aatatgtctt ttcatctgga agcgattctc ggggag
//
LOCUS       DAJTRW010000060        22150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRW010000060 DAJTRW010000000
VERSION     DAJTRW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..897)
                     /locus_tag="OO326_002545"
     CDS             complement(<1..897)
                     /locus_tag="OO326_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5966891.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTA"
     gene            complement(999..1979)
                     /locus_tag="OO326_002546"
     CDS             complement(999..1979)
                     /locus_tag="OO326_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5966892.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1987..2697)
                     /locus_tag="OO326_002547"
     CDS             complement(1987..2697)
                     /locus_tag="OO326_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5966893.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2914..4737)
                     /gene="typA"
                     /locus_tag="OO326_002548"
     CDS             complement(2914..4737)
                     /gene="typA"
                     /locus_tag="OO326_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5966894.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5110..6519
                     /gene="glnA"
                     /locus_tag="OO326_002549"
     CDS             5110..6519
                     /gene="glnA"
                     /locus_tag="OO326_002549"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5966895.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6692..7741
                     /gene="glnL"
                     /locus_tag="OO326_002550"
     CDS             6692..7741
                     /gene="glnL"
                     /locus_tag="OO326_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5966896.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7753..9162
                     /gene="glnG"
                     /locus_tag="OO326_002551"
     CDS             7753..9162
                     /gene="glnG"
                     /locus_tag="OO326_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5966897.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9274..9384
                     /locus_tag="OO326_002552"
     CDS             9274..9384
                     /locus_tag="OO326_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5966898.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9613..10986)
                     /gene="hemN"
                     /locus_tag="OO326_002553"
     CDS             complement(9613..10986)
                     /gene="hemN"
                     /locus_tag="OO326_002553"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5966899.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11175..11684)
                     /gene="yihI"
                     /locus_tag="OO326_002554"
     CDS             complement(11175..11684)
                     /gene="yihI"
                     /locus_tag="OO326_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5966900.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12266..12898
                     /locus_tag="OO326_002555"
     CDS             12266..12898
                     /locus_tag="OO326_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5966901.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13280..16066)
                     /gene="polA"
                     /locus_tag="OO326_002556"
     CDS             complement(13280..16066)
                     /gene="polA"
                     /locus_tag="OO326_002556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5966902.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16430..17362
                     /locus_tag="OO326_002557"
     CDS             16430..17362
                     /locus_tag="OO326_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5966903.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17403..18833)
                     /locus_tag="OO326_002558"
     CDS             complement(17403..18833)
                     /locus_tag="OO326_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5966904.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18988..19614)
                     /gene="dsbA"
                     /locus_tag="OO326_002559"
     CDS             complement(18988..19614)
                     /gene="dsbA"
                     /locus_tag="OO326_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5966905.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19631..20617)
                     /gene="srkA"
                     /locus_tag="OO326_002560"
     CDS             complement(19631..20617)
                     /gene="srkA"
                     /locus_tag="OO326_002560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5966906.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20694..20963)
                     /locus_tag="OO326_002561"
     CDS             complement(20694..20963)
                     /locus_tag="OO326_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5966907.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21033..21617
                     /gene="mobA"
                     /locus_tag="OO326_002562"
     CDS             21033..21617
                     /gene="mobA"
                     /locus_tag="OO326_002562"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5966908.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21599..22126
                     /gene="mobB"
                     /locus_tag="OO326_002563"
     CDS             21599..22126
                     /gene="mobB"
                     /locus_tag="OO326_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5966909.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5478 a         5546 c         5303 g         5823 t
ORIGIN      
        1 agcggtactg atgataaagg tgtagcatag atatttactc ggcgatttca ggatcttatc
       61 tgaaatcatg ccaccaatcg gtccaccaat cattttcaga cagtattggt taatgatgcc
      121 gtaagccccc accagcgcaa cgggcaatag atagatgttt ttcaggaatg gaatgaagaa
      181 tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgttg aaagcgataa gccagatggt
      241 tttgtctctc agcactgagg tcatactggt gttttgcgat gctccgtctt cttttttcac
      301 ctcaacggac ggtgcctctt ctttgtcatt caggacaaag aaaataataa tgcctacggc
      361 aatcacaata agggaataga accagatgcc tgctttaaaa cctaataagc cactgccaaa
      421 ccaggtaaat actgccaacg cagaaaatgc caccacggta tcgacaatgc cacgccctgt
      481 ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg cccaatcgac ttaccgactt
      541 gagcaagacc ggccagttca tcatgtcgca agtaacgcca aatagcgccc agacaaagag
      601 gatgccccaa taccccggca ttgtcgtcaa ataaacaccc agtaatcctg tcgcaatgag
      661 tgagaatgac atggtgtatc tgcgcggtag tttatcggca aaataaatag acagaaaaaa
      721 gcccactgtg gtgacaaatg agtttaccga catagcatta ccaatttgac cattggtcaa
      781 atggaaatat tcctgcatcg ggatataaaa cgcatctttc agcgacggta atttataaat
      841 tgtaccgcca cacagtgtta atagactaaa taacgcccat ttctttttcg tgagcataac
      901 atcccctaag cgatttttaa aatcactgtt ttaaaggcaa taagataccg accattcata
      961 tggaatggtt ttattaactg cgtatgagtt aaaacttatt agtggttcag aagatggttt
     1021 gcctcttgtt tgatttgttg tagtacgtta cgcacatgat ttatctgatt aattgcatct
     1081 tctttttctt tacgtaaccg cgcatccagt aaatgatttt ctggtagggg tgtttcactc
     1141 atctggcgat aagggatcca cggattatca tcttcgtctt caaagcggaa cgccgacgca
     1201 tagtaatcaa cctcttcttc caggccatat gccgtcacct gcggctcatc atcgccaagg
     1261 cagataaggt gcgttaataa cgctttgaat ggcatatcac cctgacctga aatacaggct
     1321 ttatgaccca ggccaccctg ttctttaacg atcaaggcat ctttaatatg gacctgggta
     1381 atatgcggtg ccatcgtttt taaggcgtcg atgggatgct catttgcatt gatcatgtta
     1441 gcaaaatcaa acagtaagga aagagactcc atctcacttt ctttgaccag cgacaccagc
     1501 tcatgacttt ttaaatcttc atgctgctca atcgtaaaag tcaggccgct gtcctgatac
     1561 gtttcccgta cataggcaat gtcgttagcg ataatcgata atacgtcgcg caaattacct
     1621 tcataacatg gataaaaacg tacggacgtt gccccagttt tcaacgcgat ggcgacggct
     1681 tcgtcaatag atgccttatc tgaagcgctg gtttcaatat gaatatcaag gttcagacgg
     1741 cgggctttat ccccaaaggc agagagttct ttatcgtcca tattaccaag agaaaaacgc
     1801 tcgccatcca gaacatggat tttgacgcca cgtagattat tttcactggc gatatcaagt
     1861 aaatcagcag gcaacactct ttccagccgc atatttaaat gaaaggcata agcatgtaaa
     1921 taaagaggaa gagtgtcgac acgttgtaga atctttcttg cgttatttat tgtcaccata
     1981 aaacacctac agattaatga tgaggtctac ggcctcaaat cgccaatatt caatatctat
     2041 ctggacaata ttgccttgct ggtcggcgcg atgcttttca attaagatcg ctggcatacc
     2101 cgcagaaccg ccaagatact tgctgatatc gcccggcatt ctgacgggtt tgaaaatcaa
     2161 tttttttact accgtctctt tttggtagca cttttcccag actgcagaaa atgagtggtt
     2221 ttcaagttgt tcaataaaac ccggagcaac ttcaggatta atatatgttt cgtgatagaa
     2281 aactttatgt ccttccagcg ccccccagcc ggtgatgcga tagagttcag ttgatggcgt
     2341 cactgcaatc agtggcgcga gcgtctcggg aatatcgctg gtacggtttt tctcggtaaa
     2401 cccccaagaa ggctctcgcc cttgctcaat tgcggcccgc tgaaagctcg ccgacagctc
     2461 cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc ttacgatcct tacggaagat
     2521 tttggattct gcctcgaggt tcaacaacgc ctggcgcagc gtcatacgtt taatgcccag
     2581 cagttctccc agctctcttt ctgaagggag ttttccaccc ggcgtggtga tgccatcttt
     2641 caaccaccga tccagtttct cttttgcatt ttctacggtg gattgattct ccgccattgt
     2701 ttaatccttt tggtttaaac cagatgaagc atcttacgct tacgccaaaa aagaaagcaa
     2761 ttaacggtgt atgactttgc agataattgt gacaaagctc tctattgacg taatcacaga
     2821 cttataaggg agatactcgc attggtgtgc gctcacgaca ggtaaaaaaa aacctgccat
     2881 cgctggcagg ttttttatga ctaaaaaacg aaattaatcg tctttcggtg cgcggttggc
     2941 gcggcgacga tcgttttccg tcaggtgacg tttacgaata cggatagagg tcggagtcac
     3001 ttctaccagt tcgtcatcat cgatgaactc cagagcttgt tccagagtca tgcggatagg
     3061 cggaaccaga acaacagctt cgtcagtacc ggaagcacgc atgttggtca gtttcttacc
     3121 ggtcaggcag tttacagtca ggtcgttaga gcggctatga ataccgataa tctgaccttc
     3181 gtaaacttct gcaccgtgac cgaggaacag cttaccgcga tcctgcagac cgaacagcgc
     3241 gaacgcgaca gctttaccct gaccgttaga gatcagtaca ccgttctgac gctgacccac
     3301 ttcgcccgga cgtacgtcgt cgtagtggct gaaggtggag tacagcagac cagtaccgga
     3361 agtcatggtc atgaactcag aacggaagcc aatcagacca cggcttggga tcacgtagtc
     3421 gagacgtacg cggcctttac cgtctggatt catgtttttc aggtcgcctt tacgttcgcc
     3481 cagcgcctgc atcacagaac cctgatgctg ttcttcaacg tccagcgtca cgttttcata
     3541 cggctcttgt ttacgaccgt ctatttcacg gaagattact ttcggacggg ataccgccag
     3601 ttcgaaacct tcacgacgca tgttttcgat aagaacagag aggtgcagtt caccacgacc
     3661 agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg cgcagcgcaa cgttgtgtac
     3721 cagttctttg ttcagacgat cgaggatctg acgagacgtt acgaacttac cttctttacc
     3781 gcagaacggc gaggtgttaa cgcagaagaa catagaaacg gtcggctcat caacggagag
     3841 tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg tcagaaatgt tcagttcgcc
     3901 caggcccgtg atcgccacga tatcgccagc ttccgccaga tcggtttcga tacgttccag
     3961 accgaggtgg cccagcactt taccgacttt cgcgttacgg gttttgcctt cgctatcgat
     4021 gatagtaacc tgctggttcg gcttcacttt accacgcttg atgcggccaa tgccgataac
     4081 gccaacatag ctgttgtaat cgagctgaga aatctgcatc tggaacggac cgtcaaggtc
     4141 aacgtccggc gcaggaacgt ggtcaacaat cgcctgatac agcggggtca tgtcttccgc
     4201 catatcttcg tggtccagac ccgcgatacc gttcagcgca gaagcgtaaa cgatcgggaa
     4261 gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga tcgaatacct gatccacaac
     4321 ccaatcagga cgcgcgccag ggcggtcaac tttgttgata acaacaatcg gcttcaggcc
     4381 gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc atcgggccgt caaatgcgtc
     4441 aaccaccagc agcactgagt ctaccatgga cattacacgt tcaacttcac caccgaagtc
     4501 ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca ttccatttga tagcggtgtt
     4561 tttcgcgagg atggtaatcc cacgctcttt ctccaaatcg ttggagtcca tcacgcgctc
     4621 ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg agcagcttgt ctaccagggt
     4681 ggttttacca tggtctacgt gcgcgatgat ggcgatatta cgcaattttt cgatcacaac
     4741 tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg attaatcgca ctacaaaaca
     4801 ggatcacaaa catcctccgc aaacaagtat tgcagagtcc ctttgtgatc gctttcacgg
     4861 agcataaaaa gggttatcca aaggtcattg caccaatatg gtgcttaatg tttccattga
     4921 agcactatat tggtgcaaca ttcacatcat ggtgcagccc ttttgcacga tgttaagcat
     4981 gataacgcct tttaggggca atttaaaagt tggcacagat ttcgctttat cttttttacg
     5041 gcgacacggc caaaataatt gcagatttcg ttaccacgac gacaatgacc aatccaggag
     5101 agttaaagta tgtccgctga acacgtactg acgatgctga acgagcacga agtgaagttt
     5161 gttgatttgc gcttcaccga taccaaaggt aaagaacaac acgtcactat ccctgctcat
     5221 caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg acggctcctc gattggcggc
     5281 tggaaaggca ttaacgaatc cgacatggtg ctgatgccag acgcatccac tgcagtgatt
     5341 gacccgttct tcgccgactc caccctgatt atccgttgcg acatccttga acctggcacc
     5401 ctgcaaggct atgaccgtga cccgcgctct attgcgaagc gcgccgaaga ttacctgcgt
     5461 tccactggca ttgccgacac cgtactgttc gggccagaac ctgaattctt cctgttcgat
     5521 gacatccgtt tcggatcatc tatctccggt tcccacgttg ctatcgacga tatcgaaggc
     5581 gcatggaact cctccaccca atacgaaggt ggtaacaaag gtcaccgtcc ggcagtgaaa
     5641 ggcggttact tcccggttcc gccggtagac tctgctcagg atattcgttc tgaaatgtgt
     5701 ctggtgatgg aacagatggg tctggtggtt gaagcccatc accacgaagt agcgactgct
     5761 ggtcagaacg aagtggctac ccgcttcaat accatgacca aaaaagctga cgaaattcag
     5821 atctacaaat atgtcgtgca taacgtagcg caccgcttcg gtaaaaccgc gacctttatg
     5881 ccaaaaccga tgttcggtga taacggctcc ggtatgcact gccacatgtc tctgtctaaa
     5941 aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc tgtctgagca ggcgctgtac
     6001 tacattggcg gcgtaatcaa acacgctaaa gcgattaacg ccctggcaaa cccgaccacc
     6061 aactcttata agcgtctggt cccgggctac gaagcaccgg taatgctggc ttactctgcg
     6121 cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt ctccgaaagc acgtcgtatc
     6181 gaagtacgtt tcccagatcc ggcagctaac ccgtacctgt gctttgctgc cctgctgatg
     6241 gccggtcttg atggtatcaa gaacaagatc catccgggcg aagccatgga caaaaacctg
     6301 tatgacctgc cgccagaaga agcgaaagag atcccacagg ttgcaggctc tctggaagaa
     6361 gcactgaacg aactggatct ggaccgcgag ttcctgaaag ccggtggcgt gttcacggat
     6421 gaagcgatcg atgcgtacat cgctctgcgt cgcgaagaag atgaccgcgt gcgtatgact
     6481 ccgcatccgg tagagtttga gctgtactac agcgtctaat agttgaagtt gtactacccg
     6541 gcgcacaacg ccgggattta gttgccgtgg aaactttcag cccatctctg catgggcttt
     6601 tttctccgtc aattctctga tgcttcgcgc tttttatccg taaaaagcta taatgcacta
     6661 aaatggtgca acctgttcag gagactgctt tatggcaaca ggcacgcagc ccgatgctgg
     6721 gcagatcctc aactcgctga ttaacagtat tttgttaatt gatgacaatc tggcgatcca
     6781 ttacgccaac cccgccgcgc aacaactgct cgcccaaagc tcccgcaaat tgtttggtac
     6841 gccgttaccg gaactgttga gctacttctc attaaatatc gagctgatgc aagaaagtct
     6901 ggaggcgggg caaggcttta ccgataacga agtgacgctg gtcatcgatg gtcgctcgca
     6961 tatcctttct gtgacggccc agcgtatgcc ggacggcatg atcctgctgg agatggcacc
     7021 gatggataac cagcgccgct taagccagga acaactacaa catgctcaac aagtagctgc
     7081 ccgtgattta gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg gtttacgtgg
     7141 cgcggcgcag ttactcagca aagcgttacc tgacccatca ctactcgaat ataccaaagt
     7201 gattatcgaa caggcggacc ggctgcgaaa tttggtcgac cgtctgttgg ggccgcagct
     7261 gcccggtacg cgcgttaccg aaagtattca caaagtggct gaacgcgtgg taacgctggt
     7321 gtcgatggaa ctgccggaca acgtgcggtt gattcgtgat tacgatccca gcctgccgga
     7381 actggcgcac gacccggatc aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct
     7441 acaggcgctg gggccggaag gcggtgaaat cattctgcgt acccgcaccg cgtttcaact
     7501 gaccttacac ggcgagcgct accggctggc ggcgcggatt gatgtggaag ataacgggcc
     7561 gggcattccg cctcatttgc aggatacgct gttttacccg atggtcagcg gccgcgaagg
     7621 tggcaccggg cttggcttat ccatcgcccg taatttgatt gatcagcatt caggcaaaat
     7681 tgaatttacc agttggccag gtcataccga gttctcggtt tacctgccta tcaggaaata
     7741 aaggtgacgt ttatgcaacg agggatagtc tgggtagtcg atgacgatag ttccatccgt
     7801 tgggtgcttg aacgtgcgct cgctggagcg ggtttaacct gtacgacatt tgagaacggc
     7861 gcggaagtac tggaggcgct ggcgagcaaa acgccggatg tgctgctttc agatatccgt
     7921 atgccgggaa tggacgggct ggcgctgctc aagcagatta aacagcgcca tccaatgctt
     7981 ccggtcatca ttatgaccgc acattccgat ctggatgctg ccgtcagcgc ctatcaacaa
     8041 ggggcgtttg attatctgcc caaaccgttt gatatcgacg aagcagtggc gctggttgag
     8101 cgcgctatca gtcattacca ggaacagcag cagccgcgta atattcagct taacggccca
     8161 acgaccgata tcatcggcga agcgcaagcc atgcaggacg tgttccgtat tatcggtcgg
     8221 ctttcgcgtt cttctattag cgtgctgatt aacggcgaat ccggcaccgg taaagaactg
     8281 gtcgctcatg ccctgcatcg ccacagtccg cgcaccaaag cgccgtttat cgcgctgaat
     8341 atggcagcta tcccaaaaga tttgatcgaa tcagaactgt ttggccacga gaaaggcgcg
     8401 tttactggcg cgaataccat tcgtcagggg cgttttgaac aggccgatgg cggcacgtta
     8461 tttctcgacg aaattggtga tatgccgctg gatgtgcaga cgcgtttgct gcgcgtgctg
     8521 gcagacggtc agttttatcg cgttggcggc tatgcgccgg tgaaagtgga tgtgcggatt
     8581 atcgctgcca ctcaccagaa tcttgaacag cgggtgcagg aaggtaagtt ccgtgaggat
     8641 ctgttccacc gcctgaacgt tatccgcgtt catctgccgc cgctgcgcga acgtcgggaa
     8701 gatattcccc gtctggcgcg ccatttttta caggttgccg cgcgcgaact gggcgtagaa
     8761 gcgaagttgc tgcatccgga aaccgaagct gctctgacgc gtctggcgtg gccaggcaac
     8821 gtgcgccagc tggaaaacac ctgccgctgg ctaacggtga tggccgccgg gcaggaagtg
     8881 ttgattcagg atttgcccgg cgaactgttt gaatcaacgg ttgcggagag tacttcgcaa
     8941 atgcaaccgg acagttgggc gacactttta gcgcagtggg cagacagagc gctgcgttcc
     9001 ggtcatcaaa atctgctttc cgaagcgcag ccagagctgg agcggacgtt actgacgacc
     9061 gcgttgcgac atacgcaggg gcataaacag gaagcggcgc ggctactcgg ctggggccgc
     9121 aacactctga cgcgtaagtt aaaagagctg gggatggagt gattcacggc ttgtgtgtaa
     9181 agattgatta ttgagcgcaa attgctggta ttttgcgctt tactgttccg ataagttcag
     9241 tatgatcttg cccggaaaac ggggagagtc attatgctgg aatcaataat taatctggta
     9301 tcgagtggcg cagttgacag ccacacaccg caaactgccg ttgctgccgt gctgtgtgcc
     9361 gcgatgattg ggctgtttag ctgaagctcg ttgtttatgc cggatgcgga gaaagtcgta
     9421 ggcctgataa gacgcgacca gcgtcgcatc aggcatcatg ctcagatgcc tgatgcggcg
     9481 taaacgcctt atccggccta caaaagcaat aatttcaaca ggttttgttg cccagcgcat
     9541 caggcaatct tgttctcagc acattgatgg cgctttcgtt ttctactttg taaacgaagc
     9601 gccattcact ctttaaatca cccgtgagaa ctgctgcatc cgcgctttct ggcgcagata
     9661 ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga cctttcgccg tcacctgtat
     9721 tcccttctca tccacatcca ccagcccatc ttttgctaac ggggcgagca gcttgagatc
     9781 ttccgcaaag taatcagcga agtgcaaatc ccactgtttc tcaagagggg cgtaatccag
     9841 acggaagttg cagatgagcg acttaatcac atcgcggcga atacagtcat cacgcgttag
     9901 cgcaatacca cgccacagcg cattgccttg ttcatacact tgctgatagt actgcttcaa
     9961 ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa cgcccatccc
    10021 cagcagatcg gtatcgccct gagtggtgta gccctggaag ttacgatgca gcacgccttc
    10081 acgctgggcc accgccagct cgtcatcagg acgggcaaag tgatccatac cgataaactg
    10141 atagcccgat tgcgtcagga aggcgatggt ttcctgcagg atatcgagtt tttgctgcgg
    10201 actcggcagg tcagcatctt tgattttgcg ctgagcagca aaaatggtcg gcagatgcgc
    10261 gtagttaaaa acgctcagac gatcggggtt cagctccgcc acacgtttca gggtaaaggc
    10321 gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg tcgatgttgg tggaggtaaa
    10381 accaatctca cgcgcatggt taagcagtgc aaagatgaac tcttcatcct gctcgcggtt
    10441 aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc atgctcaggc gattaaagcc
    10501 ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc cgcggatcga cttcgatcga
    10561 aatctccgca tcggtattga aatggaagtt ttcgcgcagc agcttcatca gacggctgat
    10621 ttgcgcttta ttcagatacg tcggcgttcc gccgccccag tgcaattggc tgacgtgacg
    10681 cccggcgaac agcggtgcac gatggacgat ttcttgctcc agcgcgtcca gatactgatc
    10741 ggccttgtgc tgctggcgag taacaatctt attgcaaccg cagaagtaac aaagcttatg
    10801 gcagaacggg atatgtacgt agagagataa tggacgctca ggatagcgcg ccacggcttg
    10861 taaaaacgcc tgttcgccga agtcttctga aaactccagc gcggtcgggt acgaggtgta
    10921 tcgtggcccg gaatagttat atttctggat cagggccaga tcccagtcga tttgctgtac
    10981 agacatgctc actccttccg atggcgtctc tggcggctac ggcgtaccgg cggatgcccg
    11041 tttcgggtca tcagccggtt gcgcagccac ttttgtcgcc gcgacaaccg ctgtagttta
    11101 acgaataacc acaccagata acatataacc ggaagggtta tcagcagaac gggaaggccc
    11161 acggcggcaa tccgttagtt gccccgcagc agacgcatca tgtcttcttg cttctcgtct
    11221 tcttcctctt cttcgtcatc gtcataagag aggccgagtt tctgcatcaa ctcatcaata
    11281 cgatccagtt tagcatccac ccaggattgc tcttcggcgc tcagggtttc gcctgcttcc
    11341 agacgttcca gcagcgcatc cagacactca tccgtttcca gtaattccaa ctccgcctgc
    11401 ggtgaaagca taggtttctc actcttcggt ttgtgctgtt tggtgacttt ttcagtcact
    11461 cccaatggaa taggggtttt actgccaata cgtggatctt ttggtgcgtt ctggcctttg
    11521 ctgcctgacg tggtgtttcc gcccgctgcg cggctgcccg gcgcatgacc acgacgtttt
    11581 ttctgacgct tacggtcacg agcttcctga tccagctcct cgcgtgtttt tcgacgcgct
    11641 tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt tcataatggt tcgtctttta
    11701 gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca aagaaaaaag
    11761 gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg tcttacaatc
    11821 cttgcaggcc gccaacacac cctgtttttc cattagccgg aacaatccgt gttgattcca
    11881 tttccgttta attacgtctc cggacgtttg tcttcctgac aatcctctgt cttcgcctcc
    11941 tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc tctatgggtg
    12001 ctactttacg cctttgcttt aaacaaacaa tcaaccgcca tcagtatttg cacattttca
    12061 gagttttata taaaagcaat tcactgattt atatagctta tttgacatgc atcgcagaat
    12121 tttctctgct atttcgcata aagactatcg gcaatgtctc acaaagcgta tggcttttac
    12181 ttacaacgct cacgccgaaa aatccgcctt ttattatggt tcaggtataa tcgccacaaa
    12241 gctttgactg acggagacga ccgctttgac taatttgaat tatcaacaga cgcattttgt
    12301 gatgagtgcg cctgatattc gccacctacc ttccgatacc ggaattgaag tggcttttgc
    12361 aggccgttcc aacgcaggta aatccagcgc gctgaacacg ctgactaacc agaaaagcct
    12421 ggctcgtacc tcaaaaaccc cagggcgcac ccagcttatc aacctgtttg aagtggctga
    12481 cggcaagcgt ctggttgact tgcctgggta cggttatgcg gaagtcccgg aagagatgaa
    12541 gcgcaaatgg cagcgtgcgc tcggcgaata cctcgaaaaa cgtcagagcc tgcaaggtct
    12601 ggtggtgcta atggatattc gccatccgct gaaagatttg gatcagcaga tgattgagtg
    12661 ggcggtagac agcaatatcg ccgttctggt gctgctgacc aaagcggaca aactggcaag
    12721 cggcgcacgt aaagcgctat tgaatatggt gcgtgaagct gtactggcgt ttaacggtga
    12781 tgtgcaggtt gaaacgtttt cttcgttgaa gaaacaaggc gtagacaagc tgaggcagaa
    12841 actggatacc tggtttagcg agatgcagcc tgtagaagaa acgcaggacg gcgaataatt
    12901 ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc ccgtaaattc aatagattac
    12961 aattctaccg ttggcctgat aaacatagcg catcaggcat tacggatctt ttctttcgcc
    13021 caataaaaaa cgccccagtc attactgact ggggcggcta aaatattcag ccaaatccga
    13081 ttacgtgaag taaaaggtct gaaagataga acatcttacc tctgtaccct acgcgactaa
    13141 ctttactctt ttttgttcag ttcagaaagc actttttgta attttttttc agttgttgca
    13201 taggaatttc taatagccat cacaaaacgt gacagcttat gttgcttact tacgaaaaaa
    13261 gggcatgttc aggcgaatct tagtgcgcct gatcccagtt ttcgccactc cccacttcca
    13321 ccagcaacgg cacatccaga cgggtacagt tttccatcag ttgatgaatc tgcttcgcga
    13381 cggcatcaac atcatcttta tgaacttcaa ataccagttc atcgtgtacc tgcatgatca
    13441 tacgtacacg cggttgctca gcctgtaacc acgcatcaac ggcaatcatc gcccgtttga
    13501 taatgtcggc ggcggttccc tgcattggcg cgttaatggc tgcacgttca gccgctgcac
    13561 gacgagcacc attgctggat ttgatatccg gcagatacag acggcgtccg tccagcgttt
    13621 caacgtagcc cagctctttc gcctgagcac gggtgcgttc catatactcc agcacgccag
    13681 ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc tttacgtgga atgttcaatt
    13741 gccgcgccag accgaaagca ctcatgccat aaatcagacc aaagttgatc gctttcgcgc
    13801 tacggcgttg ctcgctggtg acggtttcca gtggcaaacc aaacacttct gccgccgttg
    13861 cccggtggat atcttttcct tccgcgaatg cggtcagcaa gcctttatca cgcgagagat
    13921 gcgccataat gcgcagttca atctgcgagt agtccgctga gacaatcaca taatcctctg
    13981 gcgcaataaa cgcctggcgg atacgacgac cttcttcgtt acgcaccgga atgttttgca
    14041 ggttaggatc ggttgacgat aaacgtcccg ttgcagttac tgcctggtga taagaggtat
    14101 gcacacgccc ggttttcggg ttgatcatca gcggcagctt gtcggtgtaa gtcgacttca
    14161 gcttcgccag accacgatac tccagaatca cttttggcaa cggatagtcc agcgccagtt
    14221 cttccagtac ctcttccgac gttgacggcg cgccacccgg cgttttcttc agcggtttaa
    14281 taccctgttt ttcaaacaga atggtttgca actgcttggt ggaagaaagg ttaaattctt
    14341 cacctgcaat ttcatgcgct ttcttttcca gctcagccag acgcaaggtg agctcttcag
    14401 aatgattgtg cagcactttc ggatcgatct tcacaccgtt acgttcaatg cgtgaaagca
    14461 ccggcaccag cggcatttcg atattctcga agacgttcaa cggccctttg tgtttctgca
    14521 gatccggcca cattttcaga tgcaactgca aggtgacatc tgcatcttcg gcggcgtaac
    14581 gtccggcctc ttcgagggca atctggttaa aggtcagttg atttttgcct ttacccgcaa
    14641 tctcttcaaa agtgatggtt ttgtgcttca accaacgttc cgcgaggctg tccatatcgt
    14701 gacgcccggc aacgctattg agaatgtagg actccagcat ggtatcaaac gcaatcccac
    14761 gcagttcaat gccgtaattc gccagaatac cgcgatcgta tttcaggttt tgcccgacct
    14821 tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc gagtgcacgc tcgtgagaga
    14881 tttgatcggg cgcatcaaga taatcatgag caaccggaat atatgccgct acgcctggct
    14941 cgatagcaaa agaaagcccg accaggttag cagagatgtt atcaaggctg tcggtttcgg
    15001 tatcaaatgc aaataccggc gctttttcca gcttcgcaat ccacgctttc agtgtttctt
    15061 catcaaggat agtgacgtag ttgtcataag aaatcaccgt tgccgtcact tctggcgctt
    15121 cgtctgcaac actggtttcc tgaggcttcg cggctgtttt tgcccctttg gcctgtaacc
    15181 atttgcccac ttcgacatca gcagtccagc gtttgaactc atactttttg aacagcccca
    15241 acaactcttc cgctgccggt tgctgcactt ccagttgttc acaggtcagc tccagttcaa
    15301 catcggtttt aatcgtcgcc aactggtatg agagataagc aacttctttg ttttgctcga
    15361 gcttcgctgc cattgttttc gcgccacgga agctcaaccc agcaattttt tctggctcgg
    15421 catacagcgt atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt ttttcaccga
    15481 cgcccggtac gccaggaatg ttatcagagg agtcacccat cagcgccagg aagtcgatga
    15541 tcaattctgg cggcacgccg tacttattca ccacctcttc cggtccgagg atggtattcg
    15601 tcatggtatt gataagcgta atatttggcg tcaccagctg cgccatatct ttatcgccag
    15661 tgctaatcag caccggacgc ccggcttttt cggcttcgcg cgccagagta ccgataacgt
    15721 cgtccgcttc tacgccagaa accgccagca gcggcagtcc catcgcttta accatcgcgt
    15781 gcagaggttc gatttgtgct cgcagatcgt ccggcattgg cgggcgatgt gatttgtaat
    15841 gttcaaacag ttcatcacga aaggtttttc ccttggcgtc aaagaccacc gctgcatgcg
    15901 tcggtttata ttgcatgatc agactgcgca gcatgttgag gacaccatac atcgcaccgg
    15961 tcggctcgcc tgcgctgtta gtcagcgggg gaaacgcgtg atatgcgcga taaagataag
    16021 atgaaccatc tacaaggata agtggatttt gggggatctg aaccataatg tccgtgcctg
    16081 tttatcagat tatgggtaaa ggatgccaca gacaggatga aaacatgagt ttttcaccgc
    16141 ttttcgtgaa aatcttttgt agatctgctg gatcgctcgc aaatcgtcat gtggataact
    16201 ttgtgcataa attatttaca tcgcttttaa tttattatca tgctttttac agcatgatgt
    16261 ttgtctttgt ttttcatgca gttaaaaata aatccatccc taataattga gataatggga
    16321 ttttttctaa tgtggataga tatgaattat ttttctcctt aaggatcatc cgttatttgg
    16381 gtcattttct gctaaaatct gcgccttgcg cttctttagt gcactgtcta tggctaacct
    16441 tttgaataaa ttcattatga cgagaatact cgctgcgata acccttttgc tgagtatcgt
    16501 attgaccatt ttggtcacta ttttttgttc cgtcccgatc atcattgccg ggattgtaaa
    16561 actattgcta cctgtgccag tcatttggcg aaaggtttcg cgtttttgtg attttatgat
    16621 gtattgctgg tgtgaaggtc tggcggtatt actgcatctg aacccacacc tgcaatggga
    16681 agttcacggg ctggaagggt taagtaagaa gaactggtat ctgcttattt gtaaccaccg
    16741 tagttgggca gatattgtcg tactgtgcgt gctgtttcgt aagcacattc cgatgaataa
    16801 atatttcctc aagcaacagc tggcctgggt gccatttctt ggcctggcgt gctgggcgct
    16861 ggatatgccg tttatgaagc gttattcccg cgcttattta ttacgtcatc cggagcgacg
    16921 tggtcaagat gttgaaacca ctcggcgttc ttgtgaaaag tttcgcctgc atcccaccac
    16981 cattgtcaat ttcgttgaag gctcccgttt cacgcaagaa aaacatcagc aaacccactc
    17041 cacttttcaa aacttgttgc caccaaaggc ggcaggcatt gcgatggcgc taaatgtact
    17101 gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt tatccggaca ataaccgtca
    17161 gccgttcttc gatatgttaa gcggtaaact gacgcggatt gtcgtacatg tggatttaca
    17221 gcctattgcc gatgagttac atggcgacta catcaatgac aaaagcttta agcgccattt
    17281 tcagcaatgg ctgaactcat tgtggcagga aaaagaccgg cttctgacgt ctctcatgtc
    17341 atcacagcgt cagaataagt aagttttact cgcctctgaa tgagcagagg cgagtgagta
    17401 ttttaatgaa caaaacgtcc ggcacgagac ataaattctt cttcgctcat ctcctgtccg
    17461 ttaaaaacaa ctttacccgg tgtataacgc agctgcagcg aggcggtatt gttgtcaatc
    17521 gtgattaact gaaacatctg acctaatgtc atcatcccgc tgatttgttt atcagcctgc
    17581 ttttgcgctt tttccgcatc cattccttca gataaattaa gctgttttgc ggtttctgtg
    17641 acgacattaa gcggtaactt cacatcaaaa ttgagcgatt tgatatcttt gtttgtggat
    17701 gatgaagatt tggctgggtc ggcaatactg atatccagat tggcatttag ttcgccgagt
    17761 gcgttcttcc atcttaccgg ttgtttaatg gtcggctcac ttttttgtaa taacggtaaa
    17821 tactctttga acaatgcagc attcacttct tgcagagcaa cttcatcgtt tgctaactca
    17881 gggtgtgcag caagctgctt ctgcatcgca atgttatact ggatgataaa ttggcgcact
    17941 gctgagggat caatagattc agcaatcaat gaccactcac cactaccgag ttgctgattc
    18001 ccttttgtta acttatcaag ttcgtaggtc agattgacat tgatataatc tttgtcttta
    18061 acgcgatcca gcctggtttt tgcaacgaaa gcatcgattt gggcaacgtc ttccccgtga
    18121 ttgataatgt taatctgatt aaatttcgac tcgctttcac caaccggaaa tttcttttct
    18181 tctaatcgcg ccagagaatc aatggtaaaa ctttttgccg ttactttgtt cattggtgat
    18241 aattgcagaa caatatcttc gactttgcct tcaatatcca ggttagacaa tgaactacca
    18301 tcaccattaa aagtaaactg gccctcacca aaagaaaatt ttccatactc tgcaggggca
    18361 acatctaatg tcgtagagaa tgagccacca aacgcgaatc gcaatgtcgc ttccacgggc
    18421 gatttatttt tcgcggcgat aaatagcggt tgtgtcagtt cattattcac taagttcact
    18481 tttgccgctg ccagtgctgg gatgacatta ccatgcatta acattgtgat gggcaacgga
    18541 ccgtgctcaa catccacatc aaatacaact ttctggcctg gcttgatatt gagatcgggt
    18601 gcaccgttat cgaatgtcat ttgcatttga aaacgggaat taaaaaagcc tttgtcaaag
    18661 tttttataac ttaacgtcat atcgtaggca tgttcacctt tctttttcgc atcgttaaaa
    18721 tctttaataa acttttcgat accaggctga atttgcgtac ctgtgtacca tgtgccacca
    18781 ccccagatta cggctaacgc aacaataaca cctgtagctg acttacgtat catataaata
    18841 ttccctgata aaacagcaaa actttgaata tccacttatg ctgaaataat gtccagcggc
    18901 aggatgcatt atcagaatgt atttatattt attaaataat aaaaaaagcc cgtgaatatt
    18961 cacgggcttt atgtaattta cattgaatta tttttcctcg gacagatatt tcaccgtatc
    19021 agcatactgc tgaacaaaaa catccatatt gctggtatcc ataccctgcg gattcagctg
    19081 atatttaccg ttaacaaaca tcgccggaac accacgcaat tgcacatcag ctgcggcttt
    19141 ttcctgctga gcgaccagag atttcaccac gaagctgttc cacgccgcgt cgtactcttc
    19201 acctttaata cctgcattga taaatacatc gcggatatca gatgctgaac gaatggtctg
    19261 ggttttctgt acgccttcaa acagcggaac agtcactttg tcttccacgc ccagcgccat
    19321 cgccacagcc catgcctgag tcagatcttt gcccaggtca ccacccatga agttgacgtg
    19381 gtatttagtc atcttcacgc cttccggcag ttttttcttc acattatcag aaatatgcag
    19441 aacttcttca aactgatagc agtgcgggca gaagaaagag aaaaactcca gcacttgcgg
    19501 cgcgccagct accggttttt ccagggtagt gtactgttta ccatcttcat actgcgccgc
    19561 cgatgcgcta aacgctaaaa ctaaaccagc cagcgccagc caaatctttt tcatgatcta
    19621 ctctctccga ttaatacata ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct
    19681 gttctataaa agtcgccgtc tgtcgtagcc agtaatcttc cccggttaac cacgggaaat
    19741 ttttcgggaa cgcgggatca gcccaatgcc gcattagcca ggcaagataa taaaccaaac
    19801 gcatggcgcg taaaggttca atcagtccga tttcagcggt gtcgaactcg ctaaattctt
    19861 cataagcttc aataatagtt tccagttgca tccgctgctg ggctttatcg ccattgagca
    19921 acatccacaa atcctgaata gctggaccat tacgtgcatc atccagatca acaaacattg
    19981 gaccatcgcg ccagagaata ttcccggcgt ggcagtctcc atgtagccgc agaacggtga
    20041 aatcttcccg ccagtgtgct gtaacggcgg cgatcagctc atctgtcgct ttcagaaatg
    20101 ccgctttcaa cccggaaggt atcagtgtag cgtcctcaaa cagcttgcga ggctcaatga
    20161 gatattcatt cagaccgatg gtcgggcgat gaataaaaag ctgtttgcgc cccgtctggt
    20221 gcatacgccc taaataacgc ccaaccgctt ccatctgatc gatattatca gcttcgaact
    20281 ggcgaccacc gacgcttgga aaaacagcga aataaaatcc ctgatgattc aataaagtct
    20341 gaccgttaaa ggccacaggt gctgcgaccg gaacttcatc atttaccagc tgcaacgcaa
    20401 attgatgttc ttcgaggatt tgatcggctg tccaacgttc agggcgataa aatttgacga
    20461 caaaacgtcg acgctcttcg tcctgaaatt gatagacacg gttttcatag ctgttaagcg
    20521 gggtaagacc ggaatccacc cggatccctt gcttaaacag agcgtccatg atggtatccg
    20581 ggtgtagtgt ctggaaagta aaagcgctgt tgttcatccc atcatccgga aaacattacg
    20641 aatgatgaag gatatcattt cacgatgatt ttggtggcgc cgcttacaag cttttactct
    20701 ttaattacgc cgcgagcgcg taacagcgcc gttttgaagt cttcttcata atctttctgc
    20761 aaacccggga tcaccgcatc tttcgcggaa tcacgcattt tcaggtgata gatcagaatg
    20821 tcatccgtca aatccgccag ttcgccgtca aaacctgact ctttcgccag tttctgcaaa
    20881 aattgcaaca agttaaagtc tggctctttt tgccaggctg gctggaggag ttcaataact
    20941 tcattcagac gtttacattt catagtaatg ctccttactc ttgagacaga cacgttagca
    21001 gggtcaatcc cacaataaaa gaggcgatat cggtgaatct aatgacgacg ataacaggcg
    21061 ttgtgctggc aggcggtaaa gccagacgaa tgggcggcgt agataaagga ttgctggact
    21121 taaatggcaa accgctgtgg caacatgtcg ctgacgcgct taagacgcag ctctctcacg
    21181 tcgtggttaa tgctaatcgt catcaggaaa tctatcaggc aggcggtctg aaagtgattg
    21241 aagattcact ggcggattac ccaggccctc tggcaggaat gctttcagta atgcagcagg
    21301 aagctggtga gtggtttttg ttttgtccgt gcgatacgcc ttacattccc catgatttag
    21361 ccgcccggct taatcatcag cgcaaagatg cgcctgtcgt gtgggtccat gacggtgaac
    21421 gcgatcaccc gactattgct ctggtaaacc gcgctattga gcctttatta ctggaatatc
    21481 tgcaagcagg agaacgccgg gtaatggcat ttatgcgtct ggctggcggt catgcggttg
    21541 atttcagcga tcataaagat gcatttgtta acgtgaatac gccagaggag cttgcccgat
    21601 ggcaggaaaa acgatgatac cgttactcgc ctttgccgcg tggagtggta ccggaaaaac
    21661 tacgcttttg aaaaaactga tcccggcatt atgcgccaga gggatccgtc cggggctgat
    21721 taagcatacg caccatgata tggatgttga taagccaggc aaagatagct atgagctgcg
    21781 caaggctggc gcggtacaaa ccatcgttgc cagccagcaa cgatgggcct tgatgacgga
    21841 aacaccagac gaagaagagc tggatctaca ttttctcgca agtcgaatgg atacctcaaa
    21901 gctggatttg attctggtcg aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt
    21961 tcgcgatgga gccggacatc gacctgaaga attagtgata gacaggcatg ttattgctgt
    22021 agccagtgat gtgccgctta atcttgatgt cgcattactg gatattaatg atgttgaggg
    22081 gctggctgat tttgtggtgg agtggatgca aaagcagaac ggataagcaa gagtaggctg
    22141 gatcagacgc
//
LOCUS       DAJTRW010000061        21503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRW010000061 DAJTRW010000000
VERSION     DAJTRW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO326_002564"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO326_002564"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5966910.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO326_002565"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO326_002565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5966911.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO326_002566"
     CDS             complement(3121..3420)
                     /locus_tag="OO326_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5966912.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO326_002567"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO326_002567"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5966913.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO326_002568"
     CDS             complement(4632..5069)
                     /locus_tag="OO326_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5966914.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO326_002569"
     CDS             complement(5115..5456)
                     /locus_tag="OO326_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966915.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO326_002570"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO326_002570"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5966916.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO326_002571"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO326_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO326_002572"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO326_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5966917.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO326_002573"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO326_002573"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5966918.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO326_002574"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO326_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5966919.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO326_002575"
     CDS             12687..13217
                     /locus_tag="OO326_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5966920.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO326_002576"
     CDS             13329..13865
                     /locus_tag="OO326_002576"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5966921.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO326_002577"
     CDS             complement(13973..14797)
                     /locus_tag="OO326_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5966922.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO326_002578"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO326_002578"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5966923.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO326_002579"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO326_002579"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5966924.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO326_002580"
     CDS             complement(18729..19202)
                     /locus_tag="OO326_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5966925.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO326_002581"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO326_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5966926.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OO326_002582"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OO326_002582"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5966927.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21503
                     /locus_tag="OO326_002583"
     CDS             21362..>21503
                     /locus_tag="OO326_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5966928.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5402 a         5490 c         5277 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgc
//
LOCUS       DAJTRW010000062        21256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRW010000062 DAJTRW010000000
VERSION     DAJTRW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            17..790
                     /locus_tag="OO326_002584"
                     /pseudo
     CDS             17..790
                     /locus_tag="OO326_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(850..2148)
                     /gene="eno"
                     /locus_tag="OO326_002585"
     CDS             complement(850..2148)
                     /gene="eno"
                     /locus_tag="OO326_002585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5966929.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2236..3873)
                     /gene="pyrG"
                     /locus_tag="OO326_002586"
     CDS             complement(2236..3873)
                     /gene="pyrG"
                     /locus_tag="OO326_002586"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5966930.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4101..4892)
                     /gene="mazG"
                     /locus_tag="OO326_002587"
     CDS             complement(4101..4892)
                     /gene="mazG"
                     /locus_tag="OO326_002587"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5966931.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4988..7222)
                     /locus_tag="OO326_002588"
     CDS             complement(4988..7222)
                     /locus_tag="OO326_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5966932.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7270..8571)
                     /gene="rlmD"
                     /locus_tag="OO326_002589"
     CDS             complement(7270..8571)
                     /gene="rlmD"
                     /locus_tag="OO326_002589"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5966933.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8628..11384
                     /gene="barA"
                     /locus_tag="OO326_002590"
     CDS             8628..11384
                     /gene="barA"
                     /locus_tag="OO326_002590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5966934.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11616..12956)
                     /gene="gudD"
                     /locus_tag="OO326_002591"
     CDS             complement(11616..12956)
                     /gene="gudD"
                     /locus_tag="OO326_002591"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5966935.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12977..14317)
                     /locus_tag="OO326_002592"
     CDS             complement(12977..14317)
                     /locus_tag="OO326_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5966936.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14319..15671)
                     /gene="gudP"
                     /locus_tag="OO326_002593"
     CDS             complement(14319..15671)
                     /gene="gudP"
                     /locus_tag="OO326_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5966937.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16105..16554)
                     /locus_tag="OO326_002594"
     CDS             complement(16105..16554)
                     /locus_tag="OO326_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5966938.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16572..17354)
                     /gene="truC"
                     /locus_tag="OO326_002595"
     CDS             complement(16572..17354)
                     /gene="truC"
                     /locus_tag="OO326_002595"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5966939.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17354..17683)
                     /locus_tag="OO326_002596"
     CDS             complement(17354..17683)
                     /locus_tag="OO326_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5966940.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18305..18850)
                     /gene="syd"
                     /locus_tag="OO326_002597"
     CDS             complement(18305..18850)
                     /gene="syd"
                     /locus_tag="OO326_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5966941.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18918..19766
                     /gene="queF"
                     /locus_tag="OO326_002598"
     CDS             18918..19766
                     /gene="queF"
                     /locus_tag="OO326_002598"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5966942.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19878..21242
                     /gene="ppnN"
                     /locus_tag="OO326_002599"
                     /gene_synonym="ygdH"
     CDS             19878..21242
                     /gene="ppnN"
                     /locus_tag="OO326_002599"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5966943.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5227 a         5675 c         5068 g         5286 t
ORIGIN      
        1 tgcagcccaa ccaactattg tcccacaatt acagcaacaa gttacgggtc ttacgagtag
       61 cttaaactca caagaaaaga aagaactgac gcacaagtta gaatctattt tcaataatac
      121 ccaagtgcaa attgctgtat taattgtttc cacaaccaaa gatgagacaa ttgaacaata
      181 tgctacaaga gtttttgaca attggcgttt aggagatgcc aaacgtaatg atgggatact
      241 gatcgttgtt gcctagtcgg atcgcactgt ccgcatccag gtaggttatg ggctggaaga
      301 aaaggtaacc gatgctctgg ctggagatat catccgtagc aacatgatac ccgcatttaa
      361 acaacaaaaa ttagctaagg gattagagtt agctataatc gctttgaata accaactcac
      421 ttcacaacac caatatccgg ctaatccttc agaaagtgaa tcaacgtctt ccactgatca
      481 ttattacttt gctatttttt gggtatttgc agtgatgttc ttccctttct ggttttttca
      541 tcaaggcagt aatttttgtc gcgcatgtaa aagtggagtc tgtatttcag caatccatct
      601 tttagattta ttcctgttct ccgataaaac tttttccatt gctgtatttt ccttcttttt
      661 tactttcacc atatttatgg tctttacctg tttatgtgta cttcagaaaa gagcatctgg
      721 tagaagttat cattcagaca acagcggttc cgctggaggc tcaagtggcg gcgcatccgg
      781 ccgctggtaa ctccaccata aaaaatgcca gcccgaaggc tggcattatc aaatcagata
      841 aagtcagact tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
      901 cagagcttct tcgatacgaa tcagctggtt gtatttagca acacggtcag aacggctcat
      961 agaaccagtt ttgatctggc ctgcagcagt accaacagcc agatcagcga tggtagcatc
     1021 ttcagtttca ccagaacggt gagagataac tgcagtgtag ccagcatctt tcgccatctt
     1081 gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt
     1141 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
     1201 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc
     1261 agattcgtcc agaccgtctt cgatagaaac gatcgggtac tgtttggtca gttcttccag
     1321 gaagtgagtg aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt
     1381 accatctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
     1441 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
     1501 caggttcggc gcatagccac cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag
     1561 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
     1621 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
     1681 accgttgatg atgttcatca tcggaaccgg catggagtat ttgcccggag tgccgttcag
     1741 ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc
     1801 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
     1861 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
     1921 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
     1981 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
     2041 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
     2101 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
     2161 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccaaggta cgcgttgccg
     2221 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
     2281 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
     2341 agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga
     2401 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
     2461 gtaacggtga cgatgacgct caacaattgt cggcgcattg tatagctggc gaaccaggct
     2521 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt
     2581 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
     2641 cgggtactta cagtctggca caaattctgt agagttggcg ttctccatgt tggcaacatg
     2701 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
     2761 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc
     2821 gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg tttcaacatc
     2881 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
     2941 tgcttcgatc actgatttat aagcatccgg cagttcaatg tacttgccga ccataccgat
     3001 ggtgacttca cttaccggat tcgcttcttc gaagataacc tgttcccatt cggacagatt
     3061 tgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
     3121 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
     3181 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggtacag cgcgatctga
     3241 acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg
     3301 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
     3361 aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatagctt cgaggaacgg
     3421 caaggattcg atatcaccta ctgtaccgcc gatttctacc agtactacgt catgaccttc
     3481 gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgcacggt
     3541 tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt
     3601 ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc
     3661 caagtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
     3721 agtacctgga tcgacgttga tgtaaggatc cagtttcatg atggtcacat tgaggccacg
     3781 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
     3841 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
     3901 gacgatggaa taaccaagac gggaaagcag tatacccgaa catgacctgt gccacaaact
     3961 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
     4021 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg
     4081 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
     4141 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
     4201 tccacttcgc gaaaacgacg ctcgaatttt tcgttcactt tttgcaatgc gatttctgct
     4261 ttcgtcccta aatggcgagc cagattaacc gtggcaaaca gcaggtcccc catttcctcc
     4321 tccagtttag cctggtcgac aacagcctgc cgcgcctcgt acatcacctc gtcgatctct
     4381 tcgtagactt tatcaaccac cgggccaagc gtcgtccagt cgaagccaac gttggcgcaa
     4441 cgtttctgga ttttttgcgc ccgcattaaa gccggtaaac tacgaggaat atcgtccagc
     4501 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
     4561 acttcactac tgttttcggc agaactatca gcaaaaacat gcggatggcg acgctctaat
     4621 ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
     4681 tgcgcgtaaa acaccacctg gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa
     4741 tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg
     4801 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc
     4861 ataatagtga gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt
     4921 caggtgtaat accttcgagt aaaagatatc gctggatacg gctgacgcca tatccagcta
     4981 tgtaagacta actcccgtgc aaccgacgcg cgtcgataac atccggcacc tggttgagtt
     5041 tacccagcac gcgccccagc acttgcaggt tgtaaatctc aatggtcatg tcgatggtcg
     5101 ccagttgctg tttggtgtcg ctacggctgg caacgccaag cacgttcacc ttctcgttgg
     5161 cgagaatggt cgtgatatca cgtaacaacc cactacgatc attagccacc acgcggacca
     5221 ccagcgaata tccggcggag tagctctcac cccataccgc gtcaacaatg cgttctggcg
     5281 catgggagcg cagttccgcc agttgttcgc aatcggcgcg gtgtactgaa ataccgcgtc
     5341 cctgggtaat gaagccgaca atctcatctc caggaatcgg ctggcagcag cgcgcgatgt
     5401 ggtgcatcag gttaccaaca ccttcaacca ccacacgacc gttatcttta ctgcggtttt
     5461 gcggtgtgta gcttttttgc tgaagctgtt tcagcgcggc ggcgtcctgc tcttcggcac
     5521 tcggcttatt aaattgcgat tgcaggaagt tcaccatctg attgagacgg atatccccgc
     5581 caccaatcgc cgccagcaac tcgtcgacat cattgaagtt gtaacgcggc agcagatgtt
     5641 tttctgcttc tttcaggctg atccccagat gttccagctc gtcgtcaagg atttgccgcc
     5701 cagccagaat gtttttgtca cggtcctgtt tacggaacca ggcgtgaatt ttcgaacgcc
     5761 cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca gtcacggctg gggttcggct
     5821 gtttctgggt gataatttca atctggtcgc ccatttgcag ctggtaggtg aacggcacaa
     5881 tgcgcccgcc aatttttgcc ccgatgcagc ggtgtccgac atcactgtgg atgtggtaag
     5941 cgaagtccag cggcgttgat cccgcaggca aatcaacgac atcacctttc ggcgtaaaga
     6001 catacacccg gtcgtcaaac acctgactac gtacttcgtc gagcatttcg ccggaatcag
     6061 ccatctcttc ctgccacgca atcagtttac gcagccaggc aatccggtct tcatgtcccg
     6121 aacgtgcgcc gccagctgcc gcgccctctt tatatttcca gtgcgcagca acacccaact
     6181 ccgcatcttc atgcatctgt ttggtgcgga tttggatctc aacggttttt ccacccggcc
     6241 ccagaaccac ggtatgaata gactgataac cgtttggttt cgggttagcg acgtaatcgt
     6301 caaactcatc cggcaggtgg cgatagtgag tgtgcactat ccccagtgcg gcataacaat
     6361 cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc aaacagctca tcgaaggcga
     6421 ggttcttttt ctgcattttg cgccagatgc tgtagatgtg tttcggacga ccatacactt
     6481 cagctttaac gccttcagct ttcatctcag cgcgcagatg gccgacaaac tcttcgatat
     6541 agtgttcgcg gtcgagacgg cgttcatgca gcagttttgc gatgcgtttg tattcggtcg
     6601 ggtggagata acggaagcag taatcttcca gctcccattt cagttgccca atcccaagac
     6661 ggttcgccaa cggcgcgtag atattggtgc actcttttgc cgccagtaca cgttcatctt
     6721 ccggcgcatc ttttacttcg cgcagatgag caatacgctc cgccagtttg atgactacgc
     6781 agcgaaaatc atcgaccatc gccaataaca tccggcgaac gttatcgacc tgttcggagg
     6841 aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc cgccatatca cgcacgccgt
     6901 gaataaggtt aacaaccgac ttaccgacgc tctcacgcag cacatcttcg ctgactacgt
     6961 tggcatcagc cagagggaac agcagcgccg cccgcagcgt gtcaatgtcc atacttaatg
     7021 tcgagaggat ctccaccatc tcaacaccac gccacaataa cagactggca tccggatgcc
     7081 cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa gcactcacac gacttctggc
     7141 tggtaatacc cagacttgcg atccattttt ccggatcaaa ttcaccagcc ttattgatat
     7201 gtgcacttct taccgcaacc atcgtcctct cctttaggga ccagacctgc cgaaatcggc
     7261 aaatcgtaac tatttaacgc gcgagaaaag taccatcgat tccagatgtc ccgtgtgtgg
     7321 gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct gcttttaata acgcttcgct
     7381 atcccgagcc agcgttgcag ggttacagga tacataaact atacgaatag gttccagttt
     7441 tataatttgc tgcataacac ctgcggcacc tgctcgcgcc gggtccagca acactttatc
     7501 gaagccgttt ttcgcccacg gctgctttgt gacatcttct tcaagatttt cgtgataaaa
     7561 cgtcacattc tgtaagccat taagacgcgc attctgctgg cctttttcca ccagcgcagg
     7621 aacaccttct acaccgacca cactggcagc ttgtgtcgcc aatggcagtg taaagttgcc
     7681 cataccgcag aacagatcca gtacgcgatc ttcaggttgc acatccagcc attccaacgc
     7741 acgcgctacc attttttggt tcacacccgc attgacctga ataaaatcgc gcgggctaaa
     7801 agttaagcgc aacccgtttg agtcatacca gggcatctca ccagagacgg tttcgagtat
     7861 ctcactatcg ggggcgagat acagatccag gccttcagaa tgcgaaaagc gttccagttt
     7921 ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa atcatcagcg tgccgctggt
     7981 tgcctgtacc agttcaacat gaccaagatg gcgcatagct tgtaagctgc ccagacatgc
     8041 cctgactttg ggcagcaatg cttcaagttg gggcgctaaa atggggcatt gtttgacgtc
     8101 gacaatgtca ctggagcccg ctttgcgaaa ccccatctga agttgctgtg ttttcggtaa
     8161 gtagttcaga cttaaacgcg cgcggcggcg atagccccag ggaacatcgg cgatcacttc
     8221 agagacatcg tgtttcatta atcgggcgag tgccgcactt ttgcttcgct gctgtaaatc
     8281 cacgctggcg tgttgttgct gacagccgcc gcatacgcca aaatgaggac agcgtggcgt
     8341 ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta gcgcgggcat actgtttttt
     8401 atcttcagta acagcaactt ccgcgttttc ctgcggcaat aatccgggga taaataacgt
     8461 tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg aggtcgttga ctgaaacggt
     8521 tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag aattgcgcca ttggcgagac
     8581 tttctcaatt taacagtgtg accttaattg tcccataacg gaactccatg accaactaca
     8641 gcctgcgcgc acgcatgatg attctgatcc tggcaccgac cgtccttatt ggtttattgc
     8701 tgagtatctt tttcgtcgtg catcgctata acgacttgca gcgtcaactg gaagatgccg
     8761 gtgctagcat tattgagccg cttgcagttt ctactgaata tggcatgagc ctgcaaaatc
     8821 gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg ccattccgat attgttcgcg
     8881 cgatttcggt ttatgatgaa aataaccgac tctttgtcac ctccaatttt catcttgatc
     8941 cctcatcaat gcagctcggc agcaacgtgc cgtttcctcg ccagctcact gtcactcgtg
     9001 acggcgatat tatgatcctc cgcacgccga ttatttctga gagttactcc cccgacgaat
     9061 cgcccagtag cgatgccaaa aatagtcaaa atatgttggg atatattgcg cttgagctgg
     9121 atcttaaatc ggttcgcttg cagcaatata aagagatctt tatttcctgc gtgatgatgc
     9181 tgttttgtat cggtattgcg cttatttttg gctggcgctt aatgcgcgat gtaaccggtc
     9241 cgattcgcaa catggtgaat accgtcgacc gcatccgtcg cgggcaactc gacagccggg
     9301 tggaaggatt tatgctcggc gagctggata tgctgaaaaa cggtatcaac tcgatggcaa
     9361 tgtcgctggc tgcttatcac gaagagatgc agcacaatat cgaccaggcg acgtccgatc
     9421 tgcgtgaaac gctggagcag atggaaattc agaacgttga gttggatctg gcgaaaaagc
     9481 gcgcccagga agcggcgcgt attaaatccg agtttctggc aaatatgtca cacgagctgc
     9541 gtacaccact gaatggtgtt attggcttta cccgcctgac gctgaaaaca gaattaacac
     9601 caacgcagcg cgatcacctg aatacgattg aacgttcggc aaataatttg ctggcaatta
     9661 ttaatgatgt tctcgacttc tcgaaactgg aagcaggtaa gctgattctg gaaagtattc
     9721 cattcccact acgcagcacg ctggatgaag tcgttactct gctggcacat tcttctcacg
     9781 ataaagggtt ggaactgacg ctcaatatta aaagcgacgt gcctgataac gtgatcggcg
     9841 acccactgcg attacagcaa atcatcacta acctggtggg gaatgcaatt aaattcaccg
     9901 agaatggcaa cattgatatt ctggtagaaa aacgtgcgct gagtaatacc aaagtgcaga
     9961 ttgaagtgca gattcgggat accggcattg gtattcctga acgcgatcaa tcgcgcttat
    10021 tccaggcctt ccgacaggct gatgccagta tttcccgccg tcatggtggc accgggctgg
    10081 ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg cgatatttcg ttccatagcc
    10141 agccgaatcg cggttcaact ttctggttcc acattaatct cgatctgaac ccgaacatta
    10201 ttatcgaagg gccatccatc cagtgcctcg caggtaagcg cctggcctat gtcgaaccaa
    10261 actccgcagc agcgcaatgc acgctggata ttttaagtga aacgccgctg gaagtggttt
    10321 atagcccaac gttctccgcg ctgcctcccg cgcattacga catgatgttg ttaggcatcg
    10381 cggtgacctt ccgcgagccg ctaacaatgc aacatgagcg attagcgaaa gcggtatcga
    10441 tgaccgattt cctgatgctg gcacttcctt gccatgcaca agttaatgct gaaaaactca
    10501 agcaagatgg tatcggcgcg tgtctgctga aaccattaac acctacgcgc ctgttgcctg
    10561 ccctgacgga attttgtcat cacaaacaaa acacgctttt gcctgtaacc gatgaaagta
    10621 agctggcaat gacagtcatg gcggttgatg acaaccccgc taacctgaaa cttatcggcg
    10681 cattgctgga agatatggtg caacatgtgg aactttgcga tagcgggcat caggcagttg
    10741 aacgggcgaa acagatgccg ttcgatttga tcttaatgga tattcaaatg ccggacatgg
    10801 atggcattcg ggcctgcgaa ctcatccacc aactcccgca tcagcgacaa acgccggtta
    10861 tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa gctgcttggc gcagggatga
    10921 gcgattatct ggcgaaaccg attgaagaag agcgattgca taatttgttg ttgcgctaca
    10981 agcctggcag cggtatttcg tctcgcgtcg tgacgcccga agtcaacgaa attgtggtga
    11041 acccgaatgc gaccctcgac tggcaactgg cactacgcca ggcagcagga aaaaccgatt
    11101 tagcgcgcga tatgctgcaa atgttactcg atttcctgcc tgaagttcgc aacaaagttg
    11161 aggaacagct ggctggagaa aacccggaag gcctggttga tttgattcat aaactgcatg
    11221 gcagttgcgg ctatagcggc gtaccacgta tgaagaatct ctgccagctg atcgaacaac
    11281 agctacgtag cggtactaaa gaagaagatt tggagccgga gctgctggaa ctgttggacg
    11341 agatggataa tgtcgcgcgc gaagccagca aaattctcgg gtaacggacg tcttgtcgag
    11401 tagcaatcgg ctttttatga cgctggtttc agattggtga caaagtgcct gtgtttatgc
    11461 cggatgcggc gttaacgcct tatccggcct acaaaaccaa ttaaattcaa tgaattaaaa
    11521 aatgatgtag acctgataca tagcacaaca ggctattttg cgtttagcat cagtctcaaa
    11581 ccggctccag atagagccgg ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg
    11641 ttatcgaacg tccagccagg aatcagatac tgcattccca tcgcatcgtc acgcgcgcca
    11701 agcccgtgtt tctgatacag ctcatgggct ttcatcactt gatccatatc gatttctaca
    11761 cccagccccg gtttttctgg cacctgtacc agcccgcctt tgatctcaaa cggttctttg
    11821 gtcaggcgct gattaccttc ctgccagatc cagtgcgtat caatagcagt aattttaccc
    11881 ggtgcagcgg cggcaacatg ggtaaacatc gccagggaaa tatcgaagtg gttgtttgag
    11941 tgtgaacccc aggtcaggcc aaattcatgg cacatttgcg ccacacgtac cgaaccttgc
    12001 atagtccaga aatgcggatc cgccagcggg atatcaacgg attgtaacga gagcgtatgg
    12061 cccatctgcc gccagtcggt ggcgatcata ttggttgcag tcggcaggcc agtcgcgcga
    12121 cggaactctg ccatcacttc acgcccggag aaaccttgct ccgcaccaca cggatcttct
    12181 gcataagcca gcgaaccttt caggtattta ccaattttaa ttgcttcgtt cagcgaccag
    12241 gcaccgttag gatcgagcgt aatacgcgcc tgcgggaagc gttgcgccag tgccacaata
    12301 gactcggctt cttcttcccc ggccagtaca ccgcctttca gtttgaaatc gttgaagcca
    12361 tatttttcat acgccgcttc cgccaggcgc accaccgcat ccggcgtcat cgcttcttca
    12421 tgacgcaggc gataccagtc gcatgagtca tccggctggc tttgatacgg cagaggtgtg
    12481 gctttgcgat taccgacgaa gaacagataa ccgagcattt cgacttcgct acgctgttga
    12541 ccatcgccca gcagcgatgc cacgtttacc cccagatgct gccccagcag atccagcatt
    12601 gccgcttcta tcccggtaac tacatgaata gtggtacgca aatcaaatgt ctgcaaaccg
    12661 cgcccactag catcacgatc ggcaaaagta ttacgcacca gcgtcagaac gtttttgtat
    12721 tcacccagcg ttttacctac caccagcgga atcgcatctt ccagcgtttt acggattttc
    12781 tcgccgccgg gaatttcccc tacgccagtg tgaccggaat tatctttgat aatcacaata
    12841 ttacgcgtga agaacggtgc gtgtgcacca ctcagattca tcagcatact gtcatgaccc
    12901 gccaccggga tgacctgcat ttcagtaaca acaggcgtcg taaattgaga actcatattt
    12961 atgtcctttt tcagaatcaa tgacggccga aaacgggacg tttacggtca aaggtccagc
    13021 cggggatcag gtactgcatc ggacctgcgt cgttacgcgc accgcaaggc agacgtttat
    13081 aggcctcatg tgccttttgt acctgttccc agtccagttc cacgcccaga ccgggcgcat
    13141 caggaacggc aatttttccg tttttaatct ccagcggatt tttggtcagg cgacaatcgc
    13201 cctcctgcca aatccagtgg gtatcgatag cggtaggatt acccggtgcc gccgcgccca
    13261 catgggtaaa tatcgccaga gagatatcga aatggttgtt ggaatggcag ccccaggtca
    13321 gcccccagtc gtcgcaaagc tgcgccacgc ggactgcacc ggaaagcgtc cagaagtgcg
    13381 gatcggcaag tggaatatct accgcattga gcatcaccgc atgacccatt tcgcgccagt
    13441 tggtggcgat catgttagtc gcgacgggca agccggtcgc ccgtcgaaac tccgccatca
    13501 cttcacgtcc ggagaagccc tgttctgcgc cgcatggatc ttcggcatag gtaagaacat
    13561 cattcagccc tttgcataaa gaaatggctt catcaagcag ccatgcaccg ttgggatcaa
    13621 cggtaatccg cgcatccggg aagcttttct tcaatgcacg aacagtgtcg atttcttgct
    13681 cgccaggtaa caccccgccc ttaagtttga aatctttaaa gccatagcga tcctgtgagg
    13741 cttccgccag acgcacaacg gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc
    13801 actcatggtt gcccggcgta ttttccagat aaggaagatc ggttttggtc cgatcaccta
    13861 tataaaacag ataaccgagg acggtaatag tctcgcgttg cttgcctggc cccaacagtt
    13921 cgcagaccgg aacattcagc gccttaccta gcaggtcaag caaggcggct tccagcgccg
    13981 ccacggcatt aacgcgcaat tcaaaagtcc aggcaccttt gccaaaggta tcaaaatcgg
    14041 ctgcctgatt acctttatgt acctgctgaa ccactttatt caggcgcgca acttcctggc
    14101 ccagaaccat cggaatagca tcgaccagtg tttgataaat cacctctccg cccggcgctt
    14161 caccaatgcc ggtatgcccg gcgttatcgg tgagtaccac gatattgcgg gtgaagtatg
    14221 cgttatgcgc accaccaata ttgagcaaca tgctgtcatg tccggcgacc ggaatgactt
    14281 tcatatcagt aataacagga ctggattgtg tcgccatctt attgccccgc gacaggtttc
    14341 agctcgatac gcttgatatc gcccaccagc accaggtagc tcagtaccgc gattagggca
    14401 tgaacaccaa cataaatcag cgccccatta aacgagccag tcgtgccaac gatataacca
    14461 attgcgattg gcgtgacgat gccagaaatg ttaccgaaca tgttgaacag gccaccggaa
    14521 agaccgctga tctcttttgg cgcggtatct gccattactg cccaacccag cgccccgatg
    14581 cccttaccga agaaggccag cgccataaag ccgatgatca tccactcaac gttgacgtag
    14641 ttgcagaaca ccatcaccat cgacagcaac atgcccatta cgatcggtgt tttacgcgca
    14701 atgttcagcg atcccgtgcg gcgcatcagc cagtcggaaa taattccacc cagcacaccg
    14761 ccgataaaac cgcaaaccgc cggaacggaa gccacaaagc ccgctttcag aatcgacatc
    14821 ccacgcgcct gcaccagata aaccggaaac caggtaatga aaaagtaagt cagggcgttg
    14881 atacagtact gaccgatata aacgccgatc atcatccgtg acccgagcag ctgtttgatc
    14941 tgcccccact tcacgctgaa cggcacttta actttggtgc tttgctgatc catattgatc
    15001 agcgcgccac ccgcggcgat atactccagc tctttctgat ttacccccgg atgttgattt
    15061 ggctcgtgga tgactttcaa ccagataaag ctgatgacaa tgcccagacc gcccataaag
    15121 aagaagacgt gtgaccagcc cacttcatgc gtcagccagc ccataatcgg cgcgaagatc
    15181 accgttgcga agtattgagc ggagttaaaa atcgacaccg ccgttcccct ttcctgcacc
    15241 ggaaaccagg ccgcaacaat gcgactgttg ccggggaaag atggcgcttc agcaagcccg
    15301 accaggaagc gcagagtaaa cagggcaacg ataatgccga atccactaaa gatatcgacg
    15361 aagccttgca gcaaggtaaa catcgaccag ataaagatcg accagaagta gacgcgtttt
    15421 gaaccaaaac gatccagcaa ccaaccacca gggatctgcc cgataacata agcccatgag
    15481 aaggcagaga acacatagcc cattcccacg ggatcaaggc cgatatcttt ggccatttcc
    15541 gaaccggcaa tagaaagcgt agcgcggtcg ccgtagttga aggatgtgac gataaacaac
    15601 atcaccacta tccagtaacg agcattcgtg cgtttttcca cactgctcgc agcctgactt
    15661 aaagaactca ttgttgcact cctgaaaatt cgcgttagcc acgctcactc tggactgcga
    15721 catcgccagg aaatcagagg tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc
    15781 cgaatacggc gcgtgtttat atctggcaat agcagtataa aaagcgcgcc atagcggctc
    15841 accgtgcaac aacacaacat taatgcgttc aatgaggccc gattttggca ttagccctgg
    15901 acggtggaat ccacttcacg gaaatgaaaa caagaacaag aaaggaaggg taaaaacgaa
    15961 gaaataaaga agagtatgaa atggatcgct tgactctagg caaacgccag taaaaatccg
    16021 cgctatgaag cagtttttac tggcatttgc ctgaaaagat tcgattcagc accgctaaca
    16081 cgacatttac cgctcgctga catatcagga caacagcgtg ccccactgtt cgacccacgg
    16141 atttgattcc gtttccggtt ccgggttctc actggcgtca atcaacagca tttcgccaac
    16201 ccgctgagcg ctctgttcct gcaacaaggc atcgaactgt ttgccgccat tgcaaaaatt
    16261 tacataactg ctgtcgccga gcgcaatcac gccataacgc agattcggct ggaagcccag
    16321 actatctttg attccctgaa agagcggcac aatgctatca ggaaggtcgc cctgcccggt
    16381 cgtggacgta accaccagaa catacttatc ctgatagggc agccagtcgc ttaattcagg
    16441 atcttcaaat accgttgctt tgtggccctg tgcggtcaga attgcttctg cttcttccgc
    16501 cactaacagc gaatttccgt acatggtgcc gacaaaaata ccaatttccg ccataccgtt
    16561 ttctccctgg attaggaact tatctctcca tcctgacccg atggcgcact gaactcaacc
    16621 ctttcatttt caggaagcag accgcgccag ccaaattgtg ataacgcctg catccaggtg
    16681 tcgtccaggc ccgcgtgaat agtcagcggc tcgccagtaa aaggatgcgt caatgacaac
    16741 tgactggcat gtagcattaa ccgctggagg ccaaaatgct cagcaccgct gcgattctgg
    16801 cgtaaatcgc catgtttgct atcgccaata atcggatgac gcaaatgggc aagatgtcgt
    16861 cgaagctgat gtttgcgtcc ggttttcggc tccagttcca ccaggccgta gcgcgtggtc
    16921 gggtaacgtc cggtcgctac cggcatttct acggtcgcca gaccgcgata atgcgtcact
    16981 gctggctgcg ggcctttatc ttcgcgggca aatttatcag cgattttgtc cagttcttcc
    17041 accagtggat aatccaacac cgcttcttcc atcaaccagc cgcgcacaat cgcatggtaa
    17101 cgtttctgga tttggtgctg ttcaaactgt tgtgccagca gccgtccggc ctcgctggat
    17161 aatcccatca acaacacacc agaagtgggt cggtccagac gatgagcagt aaaaacatgc
    17221 tgtcctatct ggtcacgcac ggtttgcatg accactactt tctcgtcgcg atccagccag
    17281 ctacggtgaa ccagccagcc ggagggttta tttaccgcaa ccagccattc atcctgatag
    17341 agtatttcca gcattagctc gcatcatccg caaaaagagc atccagtttt tccaactcag
    17401 ccagaataag cgcgcgttgc ggatggtccg tcgccagcgc catttcataa tagggtgcaa
    17461 cagcaaaagc gcccggtaac ggctggttgt tatttaacaa atcgtgcatt cgcgggatca
    17521 gcccccactg caaccactcc agtggttcca tggtgtccat aaagaacggt tgggtactat
    17581 taaattgatg cggctggggt tcatcgtttc gccagtgctg atgttcacgc agtaacgctt
    17641 caagcgcctg caactggaga cgaacgcggt catgagtggt cataaagcaa cctcaataag
    17701 aaaaactgcc gcgaaggata gcaggaataa aaaaagggag cactgtattc acagtgctcc
    17761 cggttcgttt cgcagcattc cagctacttt tgttgctccc tgctcatcct tgacaacttt
    17821 tcctctggcc ttgcggccaa tcgttcatcc tgaactattg cttcctgctc acaccacccc
    17881 gatgtgatac ttcatcctga agtgtccctg gccttcctga cccaccgaat catcctgacc
    17941 ggttctcatt ctccatcctg gaggtgtcct ttaacgcgtc ctgcgtcatc ctcttcgctt
    18001 catccagaag cctttccctg aaacaccatc ctggtgtgtc ctgcagaagt gtcatcatcc
    18061 tgatgttcac ttcgttgtct gactccctgt cgacgaagat agaatcgtct ttttcgcgaa
    18121 gtcttacaag gcgcttacag acaatgcctt aaggaaaatt tcatatgaaa gttaagaatt
    18181 aacaatcaac attttgattt taaatgaaaa taacgattct tatcttttta agtattcgct
    18241 tatttcctgg cgatctccta cagagtctgt aagagatctc tcacaaccag atggggtatt
    18301 tggattacag aagaggctta agttgattaa ggaattccgc aagatttgag gctaaattcg
    18361 tccgtttacg tgttcccagc gtctctttgc acacttcacc ggagagatta cacaccgaga
    18421 tgacctctaa ctcttcttcc agcgtcgcga taaatagcgt gggcggcagt tttagccttt
    18481 tctgagtcac cagatgacca atcagatttt cctgcacccg acggaagtcg tcttcactcc
    18541 aggtttgcag caatgtcagt ttgatatcgc caaactgcgc gtgcatatcc ccggcaaact
    18601 gagtcgtata aaaggtatga attgtgggtt gtatcacaat atcaaaagcg cgttcaaccg
    18661 cgtttacatt ttgttccccg gtaaacggct ggggttgcca gtatacggca tcttcggtag
    18721 ttgaaataat gcacggtgaa ggaacaccgt acaattcctc acttaacggc cagcttttat
    18781 gctcttcatg ccacgcatcg cagtagcgtg cagtaaaatc tttcagggct tgtgcggtca
    18841 aatcgtccac taatttctct cttcacttaa accagataca cttgtcgttt agtttatctg
    18901 gtttatgacg gtgaaacatg tcttcttatg caaaccatca ggcacttgcg ggcctgactc
    18961 ttggaaaatc aaccgattac cgggatacct atgacgccag cctactgcaa ggcgttccac
    19021 gcagcctgaa tcgcgacccg ctgggtctga aagcggataa cctgcctttt cacggtacgg
    19081 atatctggac gctgtatgaa ctttcctggc tgaatgcgaa aggtttgccg caggtcgctg
    19141 tcggtcatgt tgaacttgat tacaccagcg taaatctgat tgagtcgaag agttttaagc
    19201 tctatctcaa cagttttaac cagacgcgtt ttaataactg ggatgaggtg cgccagacgc
    19261 tggagcgcga cttaagcact tgcgctcagg gtaagattag cgtggcgtta tatcgtcttg
    19321 atgaactgga aggccagccg ataggtcatt tcaatggcac ttgcattgat gaccaggata
    19381 tcactatcga taactatgaa ttcactactg actatctgga gaatgccacc agcggtgaaa
    19441 aagtagtgga agagacgctc gtcagccacc tgctgaaatc aaactgcctg atcacccatc
    19501 aaccagattg gggttcgatc caaattcagt atcgtggacg ccaaattgac agagaaaaac
    19561 tgctgcgtta cctggtctca ttccgtcatc acaacgagtt ccacgaacag tgcgtggaac
    19621 gcatctttaa tgacctgtta cgcttctgcc agccagagaa attgagcgtt tacgcacgtt
    19681 atacccgtcg tggcggtctg gacattaacc cgtggcgcag taatagcgat tttgtcccat
    19741 cgaccacaag actggttcgg caataaattt tttctcaatt ttgcgtgctg gattcacgca
    19801 gaaggttgtg aaaggtcatc aggcagggct attgtaatca aagggaatga cgatattcgt
    19861 cccataagga gtttttcttg attacacata ttagcccgct tggctccatg gatatgttgt
    19921 cgcagctgga agtggatatg cttaaacgca ccgccagcag cgacctctat caactgtttc
    19981 gcaactgttc acttgccgta ctgaactccg gtagtttgac cgataacagc aaagaattgc
    20041 tgtctcgttt tgaaaatttc gatattaacg tcctgcgccg tgaacgcggc gtaaagctgg
    20101 aactgattaa tcccccggaa gaggcttttg tcgatgggcg aattattcgc gctttgcagg
    20161 ccaacttgtt cgcggttctg cgagacattc tcttcgttta cgggcaaatc catcataccg
    20221 ttcgttttcc caacctgaat ctcgacaact ccgtccacat cactaacctg gtcttttcca
    20281 tcttgcgtaa cgctcgcgcg ctgcatgtgg gtgaagcgcc aaatatggtg gtctgctggg
    20341 gcggtcactc aattaacgaa aacgagtatt tgtatgcccg tcgcgtcggg aaccagctgg
    20401 gcctgcgtga gctgaatatc tgcaccggct gtggtccagg agcgatggaa gcgccgatga
    20461 aaggtgctgc ggtcggacac gcgcagcagc gttacaaaga cagtcgtttt attggtatga
    20521 cagagccgtc gattatcgcc gctgaaccgc ctaacccact ggtcaacgaa ttgatcatca
    20581 tgccggatat cgaaaaacgt ctggaagcgt ttgtccgtat cgctcacggc atcattatct
    20641 tccctggcgg tgtgggtacg gcagaagagt tgctgtattt gctgggaatt ttaatgaacc
    20701 cggccaacaa agatcaggtt ttaccattga tcctcaccgg cccgaaagag agcgccgact
    20761 acttccgcgt actggacgag tttgtcgtac atacgctggg cgaaaacgcg cgccgccatt
    20821 accgcatcat cattgatgac gccgctgaag tcgctcgtca gatgaaaaaa tcgatgccgc
    20881 tggtgaaaga aaatcgccgt gatacaggcg atgcctacag ctttaactgg tcaatgcgca
    20941 ttgcgccaga tttgcaaatg ccgtttgagc cgtctcacga gaatatggct aatctgaagc
    21001 tttacccgga tcaacctgtt gaagtgctgg ctgccgacct gcgccgtgcg ttctccggta
    21061 ttgtggcggg taacgtaaaa gaagtcggta ttcgcgccat tgaagagttt ggtccttaca
    21121 aaatcaacgg cgataaagag attatgcgtc gtatggacga cctgctacag ggttttgttg
    21181 cccagcatcg tatgaagttg ccaggctcag cctacatccc ttgctacgaa atctgcacgt
    21241 aatctcccct cccccg
//
LOCUS       DAJTRW010000063        21249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRW010000063 DAJTRW010000000
VERSION     DAJTRW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO326_002600"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO326_002600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5966944.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO326_002601"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO326_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5966945.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO326_002602"
     CDS             complement(2650..3201)
                     /locus_tag="OO326_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5966946.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO326_002603"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO326_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5966947.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OO326_002604"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OO326_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO326_002605"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO326_002605"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5966948.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO326_002606"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO326_002606"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5966949.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO326_002607"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO326_002607"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5966950.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO326_002608"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO326_002608"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5966951.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO326_002609"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO326_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5966952.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OO326_002610"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OO326_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5966953.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OO326_002611"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OO326_002611"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5966954.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO326_002612"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO326_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5966955.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO326_002613"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO326_002613"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5966956.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO326_002614"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO326_002614"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5966957.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO326_002615"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO326_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5966958.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OO326_002616"
     CDS             complement(18171..18566)
                     /locus_tag="OO326_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5966959.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO326_002617"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO326_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5966960.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OO326_002618"
     CDS             19482..20075
                     /locus_tag="OO326_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5966961.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OO326_002619"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OO326_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5966962.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21249
                     /locus_tag="OO326_002620"
     CDS             20795..>21249
                     /locus_tag="OO326_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5966963.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEG"
BASE COUNT         5094 a         5289 c         5615 g         5251 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaagg
//
LOCUS       DAJTRW010000064        21028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRW010000064 DAJTRW010000000
VERSION     DAJTRW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..697
                     /locus_tag="OO326_002621"
     CDS             <1..697
                     /locus_tag="OO326_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5966964.1"
                     /translation="QQVTEIANQTKSIAIAAKSEQQSQAEARANLALAEAVSAQQNVE
                     TTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATHKK
                     GLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVD
                     GLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTS
                     PLISTTPVAENVK"
     gene            936..995
                     /locus_tag="OO326_002622"
     CDS             936..995
                     /locus_tag="OO326_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5966965.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1312..1371
                     /gene="ibsE"
                     /locus_tag="OO326_002623"
     CDS             1312..1371
                     /gene="ibsE"
                     /locus_tag="OO326_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5966966.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1491..2924)
                     /gene="hldE"
                     /locus_tag="OO326_002624"
     CDS             complement(1491..2924)
                     /gene="hldE"
                     /locus_tag="OO326_002624"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5966967.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2972..5812)
                     /gene="glnE"
                     /locus_tag="OO326_002625"
     CDS             complement(2972..5812)
                     /gene="glnE"
                     /locus_tag="OO326_002625"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5966968.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5835..7136)
                     /gene="ygiF"
                     /locus_tag="OO326_002626"
     CDS             complement(5835..7136)
                     /gene="ygiF"
                     /locus_tag="OO326_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5966969.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7378..7998
                     /locus_tag="OO326_002627"
     CDS             7378..7998
                     /locus_tag="OO326_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5966970.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8062..9300
                     /gene="cca"
                     /locus_tag="OO326_002628"
     CDS             8062..9300
                     /gene="cca"
                     /locus_tag="OO326_002628"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5966971.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9463..10284)
                     /gene="bacA"
                     /locus_tag="OO326_002629"
     CDS             complement(9463..10284)
                     /gene="bacA"
                     /locus_tag="OO326_002629"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5966972.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10375..10743)
                     /gene="folB"
                     /locus_tag="OO326_002630"
     CDS             complement(10375..10743)
                     /gene="folB"
                     /locus_tag="OO326_002630"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5966973.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10848..11465
                     /gene="plsY"
                     /locus_tag="OO326_002631"
     CDS             10848..11465
                     /gene="plsY"
                     /locus_tag="OO326_002631"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5966974.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11478..12409)
                     /gene="ttdR"
                     /locus_tag="OO326_002632"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11478..12409)
                     /gene="ttdR"
                     /locus_tag="OO326_002632"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12616..13459
                     /gene="ttdA"
                     /locus_tag="OO326_002633"
                     /pseudo
     CDS             12616..13459
                     /gene="ttdA"
                     /locus_tag="OO326_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13456..14061
                     /gene="ttdB"
                     /locus_tag="OO326_002634"
     CDS             13456..14061
                     /gene="ttdB"
                     /locus_tag="OO326_002634"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5966975.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14109..15572
                     /gene="ttdT"
                     /locus_tag="OO326_002635"
     CDS             14109..15572
                     /gene="ttdT"
                     /locus_tag="OO326_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5966976.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15615..16628)
                     /gene="tsaD"
                     /locus_tag="OO326_002636"
     CDS             complement(15615..16628)
                     /gene="tsaD"
                     /locus_tag="OO326_002636"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5966977.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            16866..17081
                     /gene="rpsU"
                     /locus_tag="OO326_002637"
     CDS             16866..17081
                     /gene="rpsU"
                     /locus_tag="OO326_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5966978.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17192..18937
                     /gene="dnaG"
                     /locus_tag="OO326_002638"
     CDS             17192..18937
                     /gene="dnaG"
                     /locus_tag="OO326_002638"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5966979.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19132..20973
                     /gene="rpoD"
                     /locus_tag="OO326_002639"
     CDS             19132..20973
                     /gene="rpoD"
                     /locus_tag="OO326_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5966980.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5058 a         5826 c         5392 g         4752 t
ORIGIN      
        1 gcagcaggtc accgaaatcg ccaaccagac gaaatcgatc gctattgccg ccaaatcgga
       61 acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag taagtgcgca
      121 acaaaacgta gaaaccactc gccagaccgc agaagccgat cgtgctaaac aagttgccct
      181 aatcgctgcc gcgcaggatg cagaaaccaa agcggttgaa ctgaccgtgc gggcgaaagc
      241 agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt gagttagccg aagctacaca
      301 taaaaagggt ctggcggaag cagaagcaca acgtgcgctg aacgatgcta tcaacgtact
      361 ttctgatgaa caaaccagcc ttaaattcaa actggccttg ttgcaggcgc tacctgcggt
      421 aatagaaaaa tccgttgagc cgatgaaatc tattgacggt atcaagatta ttcaggtcga
      481 tggtctgaat agtggcagcg ctgcgggtga tgcaaacacg ggtaatgtgg ggggcggaaa
      541 cctagcggaa caagcattat cagccgctct ctcttaccgc acacaggctc cgctgattga
      601 ctccttgctc aatgaaattg gcgtttcagg cggttcactg gcggcattga cttcaccctt
      661 aatctcaaca actcccgtcg ccgaaaacgt aaaataaatt ttcagctatg aacgtccctg
      721 gcacaaaaag ccggggattt tttatatctg cgttccgcta aaaactgcaa atgatcaggt
      781 cgttgcaacg ttttgcgtga ccgctcgggg aaggcaaaac tgcctctggg aaagcgttgc
      841 gcgaggtccg gcgctcatca acaatcgggg agtcagcaaa gggctgaaac gggaaagccc
      901 ctcccgagga aggggcctta cagaaggaaa gggttatgat gaagctcgtc atcatactga
      961 ttgtgttgtt actcgtaagt ttcgcagctt attaacagcc aatcagaggg gggagaaatc
     1021 ctccctcacc cttgtgcctt tactttaggt tgaaaaaaaa tcagcgtcaa gaaggtttca
     1081 ttaacacata tccctggccc aaaaagccgg ggatttttta tatctgcgtt ccgctaaaag
     1141 gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgaccgc tcggggaagg caaaactgct
     1201 tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
     1261 tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt tatgatgaag
     1321 ttcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta acaactgatc
     1381 agagggggga gaaatcctcc cttacccttg ttcctttact ttaggttgaa aaaacaacag
     1441 cgtcaatagg cctgcctgct acgaagcgag atctgtgaac cgctttccgg ttagcctttt
     1501 ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc
     1561 accagcactt cgccgccgtt ggcccagact tctttactcc cggcgatctc ttctggttta
     1621 tagtcgccgc ccttcaccag taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg
     1681 tcttcttcaa acgacaccac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
     1741 tgttcgagcg ggttaacggg gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg
     1801 ttcacagcga caatcaagcg gtcacccagt ttgcgggcat ttgccagata gaagacgtgt
     1861 ccggcgtgca ggatgtcaaa gacgccattg gtcatcacca ctttttcacc gcgtttacgc
     1921 gctgccgcta cggccagctt cagttcctct tcggtcatca cgccaaagcc ggtatctgcg
     1981 cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg
     2041 accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga attacccgct
     2101 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
     2161 tcctgcgcct gggttggcat atgcagcggc gctttacccg gttgcagcag cgacataccc
     2221 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
     2281 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
     2341 ttcggtgtca gcagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
     2401 agcaccggaa cgcccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
     2461 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg attaatccgc
     2521 tcgtgcagcg gctgcggatc aacaccttcg aaaccttctt caaaatccag acggatcagc
     2581 tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg
     2641 aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca
     2701 atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt catcgccacg
     2761 ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg caccggcgct
     2821 tccggcgaga tacgactggt ggggccgtac cagtaacgat ccagcatcac atcaccaacc
     2881 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
     2941 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
     3001 ttgcccgcac cagatcacgc tctgcggtga agcaatcctc cgacacatgg cccggcaatt
     3061 cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca
     3121 tcgcttcctg ctcttccata atgtcgtttt gcgccagcag ctccagaata cgcacgttgt
     3181 ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga tattgggtaa
     3241 taaattcgat atcggtaatt ccgccttcat cggctttgat atcaaagcga ttacgatgtt
     3301 tattgccgag atgagcgcgc attttctcgc gcatttcccg cacttccgtt tgcagagttt
     3361 taccttctcg cggcagtgtc ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct
     3421 gcggatcgcc gtacactaca cgcgcacgca ccagcgcctg atgttcccac gtccaggcct
     3481 cgtttttctg ataatcggca aatgcttctg cggatgtcac cagcattccc gccgccccgg
     3541 acggacgcag tcgagcatcc acttcataca aaatgccgga agaggtacgc gtactgaaca
     3601 gatgcataat gcgttgcgcc agacgcagat aaaactgccg cccgtcgatt tcccgctcgc
     3661 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat aaggtcaaga tcggaactgt
     3721 agcctaactc ccagccgccc agcttgccgt agccgaccac cgcaaaaccg cgcccttcac
     3781 gttcgttgag gtgatttggc ttaccgtagc gggccaccat ttgaccccac gcctgctgaa
     3841 cgacggcatc tatcatagct tctgccagcc aggttaagtg atcgctcact ttcattaccg
     3901 gtagcgtacc ggcgatatcc gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac
     3961 gcagcgcctc aagctgttgt tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
     4021 gttcatcacg gtaggcatcg gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt
     4081 catccagcaa taatggataa cgcgctagct ggctggcaat catcggcgat gcggcacaca
     4141 gagaaatcaa atgtttaagc gccgcaggga attcactgag caattctaaa taggttgtgc
     4201 gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac
     4261 gcgcacagac atcacttagc agatgtggca tcagatggtc gagcacctga cgtcctcgcg
     4321 gcccgatagt acgcttatcc agctctttgc ggaaatcagc aatcatcgtt aggacctgtt
     4381 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat
     4441 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
     4501 cgccaatcaa ttcattaaac acccggcgca cattggtcat atgtgcggtc agcgccccgg
     4561 tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg ttaagctcat
     4621 cagaaggaag cgtttgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
     4681 gcaggaacag atatgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
     4741 cggcaatggc gctgagcgtt ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac
     4801 cgcgaatgag ctggaacacc tgaacgataa attcaatttc gcgaatgccg cctgcgccga
     4861 gtttgatatt gtcggtcaaa ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt
     4921 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca
     4981 tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc gctttgacca
     5041 tcgcgtaacg ctcccagtcg cgtccctgct cctggtaata atcctccaac gcggcaaagc
     5101 tcagcaccag cgggccactt tcgccaaacg gacgcagtcg catatccacg cgatagacga
     5161 agccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg cgggtaaaaa
     5221 actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa
     5281 aaatcagatc gatatcagag gagaaattca gttccccgcc gcccagctta cccatgccta
     5341 agatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac tcgcggcagc
     5401 aggcgtcata cagccagtca cgcgccgcaa caatcagcgt ctccgccaga taactgagct
     5461 gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca
     5521 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac
     5581 tgagcgcctc ctgcaaccac acgctgtaat gttgccattc gtcagcctgt ggcggttggc
     5641 tttccagttc cgtcagccac tctggatgcg caatcacgct gtcctgcaca aaatcactga
     5701 aagtaagtac tgacttcgcc tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc
     5761 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
     5821 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
     5881 tcattacgga aatgttcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
     5941 tgccagttct ccagccaggc ttgcgcgacg acaggatcat agtaacccgc cagcaacagt
     6001 attgagtcaa tatcacgcat caggcgtggc aactggtcac ggtagcgatc gcctaacggc
     6061 tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt
     6121 ttgaaggagt cgctaatttt gccctgcgct ttggcatcta aaaacggctg ccatgctttg
     6181 cttaccagcc attcggtcaa cgccagcttc gccattgccg tttcggtaga gtagaccgcc
     6241 gtcacggcag aaaccgcaga agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag
     6301 tgagtactcg ctttacgcgg cacaatgcca ccgaacagca tcagtgtatg acggactagg
     6361 ctaatggctg ccagcacctg ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca
     6421 tgatactgcc attgcgctaa caccagctcg agcgccgctt ccagcccctg ttccacatcg
     6481 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
     6541 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
     6601 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc
     6661 agctcgcaga taggctcagc aaattcaccc gctttcactt ccccctggtc gagagcgatt
     6721 tcaattcggc tgccatcgat ctctactagc catttttcgc gataaaaatc ggtgctaaac
     6781 agcgactgca cgcgggaagc gagatcggca ggcaattcgc cgttcggcca gatttccgtc
     6841 ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
     6901 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
     6961 ccacgaatac gtaagcccat atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa
     7021 atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag atggtcacgc
     7081 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatggcc
     7141 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
     7201 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
     7261 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
     7321 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
     7381 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc cgtctcacac
     7441 gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag cggtccggga
     7501 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
     7561 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
     7621 ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct ggaaaatcag
     7681 gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
     7741 gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acgggttaaa agaagaaaat
     7801 cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
     7861 ctggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg tggcgtgctg
     7921 gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg caaacgcaaa
     7981 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
     8041 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca
     8101 ttgttaggac tatcggtcaa agacagagat tgggtggtgg tcggcagtac gccacaggag
     8161 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa
     8221 acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg ttacaccggt
     8281 tttacttgct ataccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
     8341 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
     8401 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
     8461 cgcgtactgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt tcgtattgcc
     8521 gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
     8581 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc ccaggtgttc
     8641 ttccaggtac tgcgcgattg cggcgcactt cgcgttttat tcccggaaat tgacgcactg
     8701 tttggcgttc cggcacctgc caggtggcat ccggaaatcg acacgggtat tcatacctta
     8761 atgacgctct caatggcggc gatgctgagt ccgcaggttg atgtccgttt cgcaacttta
     8821 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca tcatgggcat
     8881 ggcccggcgg gtgttaagtt agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa
     8941 attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccgcac cttcccaatg
     9001 ctgaacccaa aaaccatcgt caaattattt gattccatcg atgcctggcg taaaccgcag
     9061 cgtatcgagc aactggcgct gaccagcgag gctgacgtgc gcggcagaac cggttttgaa
     9121 tcagcggact acccgcaagg ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg
     9181 ccgacaaaag cggtcgttga agcgggattt aaaggcgtgg agattcgcga ggagctgacc
     9241 cgacggcgga ttgcggcggt agccagctgg aaggaacaac gttgcccaaa gcctgactga
     9301 gtataaaact aaacgttagc acgaaccgtc ccctcgcccc ttcggggaga gggttagggt
     9361 gaggggaaca gaccagcacc ggtgcgaaca atcaccccca ccccggccct ctccctacag
     9421 agcgagggag aaaacccggc ctgctcccga accttttctc aatcaaaaga acacgacata
     9481 caccgcagcc gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat
     9541 caattgcagg aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac
     9601 ggcaaacatc gggatatcgc cggttgtcag gaagccccag cttttgtaga gatcgagcgc
     9661 cgttgcgccc atcatcatcg gtaccgccag caggaacgag aactcggaag cagcgtaacg
     9721 gctcacgccc atcagcatcc cacctgaaat ggtcgccccg gaacgggaga aacccggcca
     9781 cagcgccaga cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag
     9841 acccggcgca cgcggctctt tcggcttcag gcattcggcg gcaatcagca acaaaccgcc
     9901 aacgaccagc gcatacatca catttatcgg gttaaacaat gacttaatcg tgtcgtggaa
     9961 taatagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg
    10021 acctttgctt tcaccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa
    10081 cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacttc
    10141 aaaggttttc gccgtgtcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc
    10201 cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat
    10261 cagcagcgag tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt
    10321 ccgaactact gcagtatgac caggttataa ccgtttggtt taacagctgt aaaattaatt
    10381 attttctttc agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac
    10441 tgcgcctggc ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag
    10501 ctccgccacc tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct
    10561 gaccaccgtt tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc
    10621 cgctttacgg ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg
    10681 ttcccagtcg taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc
    10741 catcacgtcc tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt
    10801 atccacagat tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg
    10861 cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt
    10921 gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca
    10981 atgtgttacg tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga
    11041 aaggaatgtt gcctgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct
    11101 taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcggt tttaaaggag
    11161 gaaaaggcgt tgctaccgct tttggagcaa tcgcaccaat tagctgggat ctcaccggag
    11221 taatggcggg aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga
    11281 ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc
    11341 cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt
    11401 ggcgtcgtca ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg
    11461 agtgatttct ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc
    11521 tggcaccatg ccgccagaaa ttccgcgcag acacgaattt catgctgcga tagagcagct
    11581 cccggtaaac agcccagata ttggcgcttt gtgcatactc cggcaatact tgcaccagtt
    11641 tgccactctc cagaaacggc aacacatccc actcggaacg cagcataatc cctttgccct
    11701 ccagcgccca ttgcaacact atctcgccgc tattggagga aagatgcccg ctaaccttca
    11761 ccgatttttt ctcctgaccg ttccccaact cccatattcc gtgggtcata tcgcgctctt
    11821 tggtcaccag acagtcatga cgacttaatt cctgtaatga ttgtggctgc ggatattttt
    11881 gcagatactc aggtgctgcg cataatattc ttttattttt cgttaacaga tgcgcaatat
    11941 aataatcagg aatttcgtca ttaatacgaa tatccagatc aatattatcc tgcactaaat
    12001 caatttgccg atcgaacaat tcaaaatgca cctgtaactc aggataattg cgcataagtt
    12061 cggtaatggc tggcgcaata tggctgcgcc caaaaccaaa actgcaacca atacgaatca
    12121 tcccctccgg gcgcgttttg atttgcgtga cgtcatcgac cagtcgctga tattgggtga
    12181 ggatttccag cgcatgttcg taacaacgtt ggccactttc agtcagcgcc acgccgcgag
    12241 cggagcggtt cagcagcgtt gttgccagcg tattttcaag gatttggatc cgcttggtga
    12301 caaatgcagg cgtttgccca agcgtcgccg ctgcagcact aaaactcccg ctgtggacga
    12361 tttctaccag tacctgcaag tctttggcta aaggccagct attcagcatc gttaattatc
    12421 cgcagttgtg ataagcgcag tgtatttcgc aaaacattgc cattccctgc tctggcgcaa
    12481 tattcattct tattttcgcg ttgattcacg aactgttaat tacatattaa cggtggcaaa
    12541 aaatataaaa ccacattttt agtgttagtt tgtggcggtg aatttttcca gacaaataca
    12601 aaaactggag ttgccatgat gagcgaaagt aataagcaac aggcagtgaa taagttgaca
    12661 gagattgtcg ctaactttac cgccatgatt tctacccgaa tgcctgatga cgtggtggat
    12721 aaactaaaac agctaaagga tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg
    12781 atgttcgaca acatgcaaaa agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg
    12841 gagattatgt tcttcgttaa agtcggttcc cgcttcccac tgcttggcga gctgcaaagc
    12901 atactcaaac aagccgtgga agaggcaacc gtcaaagcgc cactacgtca caatgcggta
    12961 gaaatttttg acgaagtaaa caccggcaaa aataccggta gcggcgtacc gtgggtcacc
    13021 tgggacatca tccccgacaa tgacgatgcg gaaatcgaag tttacatggc aggcggcggc
    13081 tgcacgctac ctggccgctc gaaagtgtta atgccgtcag aaggctacga aggcgtggtg
    13141 aaattcgtct tcgaaaatat ctccaccctc gccgtaaacg cctgtccacc ggtactggtg
    13201 ggcgtgggca tcgccacctc ggtggaaacc gccgccgtac tctcgcgtaa agccattttg
    13261 cgcccgattg gcagccgcca tcccaatcca aaagcggcag aactggagct acgcctggaa
    13321 gaaggactca accgtctggg gattggtcca caagggctga ccggcaacag ttcagtgatg
    13381 ggcgcatcgt cgcggcacgc tgctggttca tgccgatctc acctttgaaa atctgtctca
    13441 cacccggagc gcgttatgaa aaagatcctg acaaccccga tcaaagctga agatctgcaa
    13501 gatattcgcg tcggcgatgt gatctacctg accggtacgc tggtgacctg ccgcgacgtt
    13561 tgtcaccgcc gtttgatcga actgaaacgt ccgatccctt acgatctcaa cggcaaagcg
    13621 attttccacg ctggccccat cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc
    13681 ggcccgacaa ccagtatgcg tatggaaagt tttgaacgtg aatttattga gcagaccggc
    13741 gtaaaactgg tggttggcaa aggtggtatg gggccgctga ccgaagaagg ctgccagaaa
    13801 ttcaaggcgt tacatgtgat tttcccagca ggctgcgcgg tgctggcggc aacccaggta
    13861 gaagagattg aagaagtgca ctggacagaa ctcgggatgc cggagtcact gtgggtctgc
    13921 cgggtcaaag agttcggccc gctgattgtc tctattgata cccacggcaa caacctgata
    13981 gccgaaaaca aaaagctgtt cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag
    14041 cacgtccatt acatcaaata accctcccgg agaggctcac ccctctcctt tttcgcaggc
    14101 ataacacgat gaaaccttcc actgaatggt ggcgatatct tgcgccgctg gcggtcatcg
    14161 ccattattgc tctacttccg gttcccgcag ggctggagaa ccatacctgg ctctactttg
    14221 ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt gccgtggtgg
    14281 cgatggtggg tatctccatc atcgccatac tctctccctg gctgctgttc agccccgaac
    14341 agctcgccca gccaggcttt aagttcaccg ccaaatccct ctcctgggca gtttctggtt
    14401 tttctaattc ggttatctgg ctcattttcg ccgcctttat gtttggcaca ggctatgaaa
    14461 aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatgggg catcgcacgc
    14521 tgtttctcgg ctatgcggtg atgttctccg agctgatcct ggcacctgta acaccgtcca
    14581 actcggcgcg tggtgcgggt attatctatc ccatcatccg taacctgcca ccgctctatc
    14641 aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg tggatgggga
    14701 ttgttgccga ctgcgtgacc agcgccattt tcctgacggc gatggcacct aacttgctgt
    14761 taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac tggttcctcg
    14821 ggatgttgcc gctcagcatt ttactggttc tgctggttcc ctggctggct tacgtgctgt
    14881 acccgccggt actgaagtct ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg
    14941 caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg
    15001 tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac agcgtggtgg
    15061 cgctgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagcggcat
    15121 ggaacgtttt cttctggctg gcctcgctta tcaccctcgc gaccggactc aacaataccg
    15181 gttttattag ctggtttggc aaactgttag caggcagctt aagtggttat tcgccgacga
    15241 tggtgatggt gacgttgatt gtggtgtttt atctactgcg ctactttttc gccagcgcca
    15301 cggcgtatac ctgcgccctc gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa
    15361 tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta
    15421 caccatacgc caccggaccc agtccgattt actacggtag tggttatctg ccaacggcgg
    15481 attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg gtgattaccg
    15541 gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa attgctctcc
    15601 ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg acgcacgcta
    15661 acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc
    15721 gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc
    15781 atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc
    15841 agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa cgtatcgacc
    15901 accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca
    15961 cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg
    16021 tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc cattttcgac
    16081 agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc aaacgcttca
    16141 ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc agtcacgctg
    16201 attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc cggcgggtta
    16261 tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat tgccggaacg
    16321 ttccaggcaa acgccagaga acgccccacg gttgcgccaa ccagcagtgc gccgactaat
    16381 ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt
    16441 agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc cagttcaggc
    16501 acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa
    16561 cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt
    16621 acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg
    16681 atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag
    16741 tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc
    16801 accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga
    16861 ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct
    16921 tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg
    16981 aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga
    17041 aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc
    17101 tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt
    17161 ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat
    17221 taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa
    17281 aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcatt
    17341 caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc
    17401 gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc
    17461 agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg
    17521 ccatcagagg caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc
    17581 tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca
    17641 cgaggttatc gctcgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa
    17701 gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac
    17761 taacgatcag ggacgcagtt acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga
    17821 taaacgcggt cgggtgattg gttttggcgg gcgcgtgctg ggcaacgata cccccaaata
    17881 cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg gtctttatga
    17941 agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt
    18001 ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac
    18061 cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg
    18121 cgaccgtgca ggccgcgatg ccgcatggcg agcgctggaa acggcgctgc cttacatgac
    18181 agacggccgt cagctacgct ttatgttttt gccggatggc gaagaccctg acacgctggt
    18241 acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc cactctccgc
    18301 atttctgttt aacagtctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg
    18361 tttgagtacg ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata
    18421 tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt
    18481 aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac
    18541 catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc
    18601 gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa
    18661 cacttgtctc tcccagccag gtctgaccac cgggcaactt ttagagcact atcgtggtac
    18721 aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat
    18781 tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg
    18841 ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga
    18901 gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga
    18961 gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc
    19021 tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat
    19081 cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa
    19141 aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc
    19201 tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac
    19261 atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat
    19321 gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg
    19381 ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt
    19441 gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt
    19501 gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc gatcacctat
    19561 ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc
    19621 ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct
    19681 gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac
    19741 gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa
    19801 ctacgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct
    19861 gccgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg
    19921 aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa
    19981 gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt
    20041 accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac
    20101 aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa
    20161 ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt
    20221 cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac
    20281 ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac
    20341 ctgattcagg aaggtaacat cggcctgatg aaagcggttg ataaattcga ataccgccgt
    20401 ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgttctatc
    20461 gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc
    20521 aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg
    20581 gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag
    20641 ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc
    20701 gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca
    20761 acgcacgacg tgctggctgg cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc
    20821 ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc
    20881 cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt
    20941 tctgaagtgt tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc
    21001 gcacccggca ctagtctctc tgcacaaa
//
LOCUS       DAJTRW010000065        21018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRW010000065 DAJTRW010000000
VERSION     DAJTRW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(138..566)
                     /gene="mxiM"
                     /locus_tag="OO326_002640"
     CDS             complement(138..566)
                     /gene="mxiM"
                     /locus_tag="OO326_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5966981.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(550..957)
                     /gene="mxiM"
                     /locus_tag="OO326_002641"
     CDS             complement(550..957)
                     /gene="mxiM"
                     /locus_tag="OO326_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5966982.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(950..1645)
                     /gene="mxiN"
                     /locus_tag="OO326_002642"
     CDS             complement(950..1645)
                     /gene="mxiN"
                     /locus_tag="OO326_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5966983.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1587..2114)
                     /locus_tag="OO326_002643"
     CDS             complement(1587..2114)
                     /locus_tag="OO326_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5966984.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2111..2836)
                     /gene="mxiJ"
                     /locus_tag="OO326_002644"
     CDS             complement(2111..2836)
                     /gene="mxiJ"
                     /locus_tag="OO326_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5966985.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2842..3135)
                     /gene="mxiI"
                     /locus_tag="OO326_002645"
     CDS             complement(2842..3135)
                     /gene="mxiI"
                     /locus_tag="OO326_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5966986.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3148..3399)
                     /gene="mxiH"
                     /locus_tag="OO326_002646"
     CDS             complement(3148..3399)
                     /gene="mxiH"
                     /locus_tag="OO326_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5966987.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3407..4522)
                     /gene="mxiG"
                     /locus_tag="OO326_002647"
     CDS             complement(3407..4522)
                     /gene="mxiG"
                     /locus_tag="OO326_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5966988.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4531..4989)
                     /gene="ipgF"
                     /locus_tag="OO326_002648"
     CDS             complement(4531..4989)
                     /gene="ipgF"
                     /locus_tag="OO326_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5966989.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(4989..5351)
                     /gene="ipgE"
                     /locus_tag="OO326_002649"
     CDS             complement(4989..5351)
                     /gene="ipgE"
                     /locus_tag="OO326_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5966990.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5361..6977)
                     /locus_tag="OO326_002650"
     CDS             complement(5361..6977)
                     /locus_tag="OO326_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5966991.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            7291..8775
                     /gene="icsB"
                     /locus_tag="OO326_002651"
     CDS             7291..8775
                     /gene="icsB"
                     /locus_tag="OO326_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5966992.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            8788..9177
                     /gene="ipgA"
                     /locus_tag="OO326_002652"
     CDS             8788..9177
                     /gene="ipgA"
                     /locus_tag="OO326_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5966993.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9193..9819
                     /gene="ipgB1"
                     /locus_tag="OO326_002653"
     CDS             9193..9819
                     /gene="ipgB1"
                     /locus_tag="OO326_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5966994.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9876..10343
                     /gene="ipgC"
                     /locus_tag="OO326_002654"
     CDS             9876..10343
                     /gene="ipgC"
                     /locus_tag="OO326_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5966995.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10349..12091
                     /gene="ipaB"
                     /locus_tag="OO326_002655"
     CDS             10349..12091
                     /gene="ipaB"
                     /locus_tag="OO326_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5966996.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            12111..13202
                     /gene="ipaC"
                     /locus_tag="OO326_002656"
     CDS             12111..13202
                     /gene="ipaC"
                     /locus_tag="OO326_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5966997.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            13253..14251
                     /gene="ipaD"
                     /locus_tag="OO326_002657"
     CDS             13253..14251
                     /gene="ipaD"
                     /locus_tag="OO326_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5966998.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            14260..16161
                     /gene="ipaA"
                     /locus_tag="OO326_002658"
     CDS             14260..16161
                     /gene="ipaA"
                     /locus_tag="OO326_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5966999.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSYPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            16165..16401
                     /locus_tag="OO326_002659"
     CDS             16165..16401
                     /locus_tag="OO326_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5967000.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            16618..17547
                     /gene="virB"
                     /locus_tag="OO326_002660"
     CDS             16618..17547
                     /gene="virB"
                     /locus_tag="OO326_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5967001.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(18493..19272)
                     /gene="ipaJ"
                     /locus_tag="OO326_002661"
     CDS             complement(18493..19272)
                     /gene="ipaJ"
                     /locus_tag="OO326_002661"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5967002.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(19872..20009)
                     /locus_tag="OO326_002662"
     CDS             complement(19872..20009)
                     /locus_tag="OO326_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967003.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            20625..>21018
                     /locus_tag="OO326_002663"
     CDS             20625..>21018
                     /locus_tag="OO326_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5967004.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTM"
BASE COUNT         7161 a         4306 c         3144 g         6407 t
ORIGIN      
        1 ccttccatgc tggagccagc tcaattatgt tacgttcttt atcttttaca agctcaacgt
       61 taactggttc atgtccacta gagtaaatgt aaaacatatt acttgtattt agacctttat
      121 atttactcac aatacaatta tatccctcga attaatttta atttaacttc cccatccttc
      181 tctataagga tggtatcatt tatcttatta ccttttaaaa caaccccgtc tttcagcata
      241 gaaaatagtt tatctatagc tatcttttgt gcatccatga agcacccatc tacttcctta
      301 acattaaagt tgcccatgaa tttacctgca ttaaaactat aaaccgcctt accactaata
      361 catttagagt aaacatttat acttttattg gttgtattaa acgaccataa taaatcattt
      421 ggaatagaaa aataatcctt acttacagga acaatatgcc attctttttc agaattagat
      481 gatgacttta aagcacaccc actcagtgtt aatagcaata tacttaaaat aaatattttc
      541 aacttattac taccatgtcg aatcatctgc ctctctccct gcttcaaaga cactaattaa
      601 tgaatcgaag gttgtagtct taatatagaa atcaatattg ttatatatgg acaatgatgc
      661 ataaaatccc tcatgcaaat cgacatattg atttgcatct atgagtttat taagaagtga
      721 acttttttgt gcagctgtaa gataaggaaa acgtgaattg aataattgtt caatcttatt
      781 gtgcagttca tcattatcta tggaatttaa ttcactctgt gagaaatatt gcattatttc
      841 atagattata tcctcatcaa atgattgcga agatgataat ctctcattta aattaacaac
      901 ttcttcacta tttaatcttt gctgcagagc attgcttgca tttatttgat taatcattaa
      961 caggattctc ttttaacaag tcttctgcaa tttgtctcag tttcttatgt gctaactctt
     1021 tgaaatattt tttatcattt tttgttaatt tttccgcaag ttctatttta acacctgata
     1081 ttacatcctg aggactaaac tccactactt gattaccact acagaatata taattattcc
     1141 cttcagaata ttttaaaata atcttgacat ctgagcgata tttatggaga tcaagttcta
     1201 actttttcct cagtgctaaa ttacacttgg gcaaaaccac ttttaattct gttactgtat
     1261 tacgcaacga actaagccac tgctccaata aagtgctaat gatcctctca tcatgtaaat
     1321 tttctgaaag caaactagtt atatagtcct caatatttct tctattttca ttttgcttta
     1381 acttccattc acaaatatag tcaataatat gctcaaaagc agtctgtata ccaatttgat
     1441 aaccatcaca tacagcatct atacgcagtg attcagcttt tttcgtagcc tcctttataa
     1501 cacaaattgc tttcttctta ctctcagtgt ctctatcttt tatggtttta cataattcct
     1561 tttcaattct ctttataaca acaccatcat aggcatgatg tctggatacc ggtaaagtgc
     1621 ctttttgcat attgcatacc ttcatttaat cgggacaaat ttctttctac aggcgtataa
     1681 tttccctcca aagcaattgg attaaataat aagtttattc tttcatatat accaacagat
     1741 atattttcct tccacgcaat aagatgccaa aaaccacttc gaatagcggc ctccccttgc
     1801 ttactgattt tgcccccata ataactaata accatctcac tatgattaat taaccgagac
     1861 tcttgactac cgataaaata agcaacagct ggaagtaaat tccagttggc aacaaacata
     1921 gcggttaccc cgcttaaagg ttcaatatcg caatttagat catatttatt aattatgaga
     1981 ttattaatct cacttctgat tacgccattt tctaacagtt cagaaggcaa attcaaccga
     2041 tttctattta tatagaacgc tggatcaaaa attattgaaa gatatttttt ataaattcca
     2101 tccattctta tcatattttg tttctcttga accaccctgt tttgaatgcc cataccaaaa
     2161 gaatgacaac tagtacagcc atcccgagaa ataaatatat tacttcattt gttaaaaatt
     2221 ccgatttaac ttccttaaca ggttgtacat ttgtataaac atattcttct ttcggagtta
     2281 atatgacaga tatattttca tacttaacat cagaaaaggt gtttttcaaa aatcgcttaa
     2341 tattactaac taatagttca gactctttcg gtgagtcata tatagcgatt actgatatat
     2401 gcatcggttt tgaagatata tttttttctt caagatcata gctaacatgt atttttgccg
     2461 aaataacacc accaatagaa actaaagact gttccagccg ttgctcaata gcactatata
     2521 aacgggcctt ttcagctctt ggagaagaca ctaatgaatc tgtaggaaac atttgtgaga
     2581 tatcaactct ctccggattt ggcaaatcgt acatgcgcat caaatcaact gccgatgcaa
     2641 atgtcccctt ttcgacttgt accgagatcc cctgtttacc tccatcaact tttctagcag
     2701 taatattatg gcgttctagc acagatatta tttcatttgc ctgtctttga gataaattag
     2761 aaattaactc ttcacgttgc tcacatccaa tcaacatcag caacaagaat aaaataaaac
     2821 ctttatacct aatcatataa attaagactt taataaagtt tcaacagccg aaactgtttt
     2881 tcttgctaac gtgccagcta atgatactcc tattgaataa tttgagagcg tcgtctgtaa
     2941 cttggccaaa gattctgggt ttaatgattc tggattgctt accatctcca ttatttgtga
     3001 tacttgaata tctgtatcca tcttaatatc actatattta gccgacacga cgtcttccag
     3061 acttgatatc tcttgagatt gaaaatcact ggctttgata atatcaacct gattgactgg
     3121 ataaatgtaa ttcatgaatg ctccctatta tctgaagttt tgaataattg cagcatcaac
     3181 atccttaatc actttcactg tattggattg cgcgttccta tataatgtat attcagataa
     3241 tttactttgg tattcagcca gcaactgtgg attcgaagga tttttagcta atttatctag
     3301 tgccaatgtt agttcacctt gtaatgtttg agttccatca tcaaaagttt cagataatga
     3361 actcaatgtc caatcatcat tcggtactgt aacactcatt ttatcctcac ttatttttat
     3421 ctaaaaaaaa ccagtgttta tcattcaaca ttacataact gtctttgctg ttaagatatg
     3481 atttaccttt aaaatcatca tctatcaaaa gtacagaaaa ctcaatatac tgatctccat
     3541 atatattttt atgttcagaa atcagtctat taatgagctc aagcttttca tctgtcagtt
     3601 cttctctgac agaatatgta actttattat tttcacaaat ctctttatac tggacattta
     3661 cctttgtgaa aataccttta gcatcccccc ttgcattatg atcgctaaga tagttgaatt
     3721 taatattttt gatatacgga aattcaacaa tcagagcatt tttcaattta tcaagttcgg
     3781 tatcttttga actatttctt tctttactta aaaacaccag taattccgct ttatcactca
     3841 ctaaattcaa cacatacaaa ttgataaatg ggaatcgttg attgatatat tgttgaattc
     3901 tatttatttc tttgtttgac accagaatat atctgcattt ggtaaatccg gttttattta
     3961 aggctacaga cgcccatacc gcatcatttt gtgtttcggc aagaatataa ttgcactgtc
     4021 cagtgttgac tattccatat ctcttatcat ctataatttc agcaatttct tttacttcat
     4081 ctttctttaa agagagggaa aaaattataa ttaacacaac aataacagca aagaaaaaaa
     4141 acaccgagtg gttcttatac attccgttta gtatatggcc tctcgattta tcctccctca
     4201 tatttttaag gtgaaaagaa atcccgtcat attcaaatga attaaattgt gaattgatat
     4261 caatttgctc acctttttca tcactaaatt gacagatatt atctttgctt atggtcaaat
     4321 aaattatacc atccgattta ctatctgtta caggaataat attttctggg aaattgtcgt
     4381 ttccaaactc aagtgtttct atcgtccgtc cgagaacaat taagttattc ccataatata
     4441 acggaaactc atccccgaca ccgtttgtga gtttcactaa taacctgaat ggtgcaagat
     4501 ttgagttctt tgcctcagac ataaatcctc ttatatcctt cgattattct gcttgctctc
     4561 tgctgctatc cgcaaatagc gtctatagat atcctctgcg tactttaatc tttctttttt
     4621 ctttttcgga gacgttcctg cattataggc tccaacagcc tcccaccctc ttccatacat
     4681 catcataaac tcatttaata actttgctgc ataatgtaca gaaagacatg gatgactaat
     4741 tagcatttcc tcagaatatc ccatttctct aagtctttta gaatgaaagt catttatttg
     4801 cattatacca taatctttac ttccattgtt attaacattg actgcagatt tattaaatcc
     4861 ggattctttt tccgcaatcg cttttaacaa acttgatgga atattgtacc tttcaccagc
     4921 cttatcccaa caatcagctc tccctaaata tgggataaaa cacagcaaaa taaatacgaa
     4981 acgggacatt aatacccctt cattcttcgc gcaaattcat ccttcagcca acgcacacgc
     5041 gaaatataat attctgtatt aacatataca tcctcgaatt catttatccc ggtaagatct
     5101 aatcgcgcaa ttagatttcc gccttcatca tcagtagcta agcatatctt ttctgagtaa
     5161 tttaggctca acgcatatat aagatcattt atattttcag gcaatgcaca aaaagggcat
     5221 aataaattta ttgaatctcc ttctttttca atatatatta gcacatcatc atcaagcatg
     5281 attgcttgat catcgatatc aattaaaggg atacccaagg ctcggcaaat aacatctgct
     5341 aaatcttcca tatattcctc ttatacaaat gacgaatacc ctttcaccat attccatatt
     5401 tttgggtccc ctattctttc agaataagat agctctaacg aagatagcgg caattttttc
     5461 atgactttat ttccgggcac accagtattc atctcttgga tttccatatt accactattc
     5521 attagaatag tagaaaataa tcttttctct tctgaggata atttactatt taattgagaa
     5581 aactgacctg tttcatgttt tcttattatt tctcttttaa tttcagcatc ctgcatacct
     5641 gttctgtcct tcccactctt acaattccag caaggtacag cacctatagt ataagccaat
     5701 agagtcattc tttgtgacaa tttatacggc tctccattat catttttttg taactttttg
     5761 gttacaatct cttttatctg gttagccaga tatatcacat cattcttaca tggtggattt
     5821 ttttctattg cttctgcagc ccaacctcca atcacaccat ttttgaggaa attatccccc
     5881 aacaaagaac atattgattc atcattaagc ttgtcaacat tcctccagcc taagcccatt
     5941 tttagcgcta attcatttac accaaagtta aatgtcacca cttttagatt aacgcttact
     6001 tctttcagaa gaccatcact attccgaatt aataatttcg ttggcttccc ccttttagag
     6061 tttaaccctt ttaatgcgtt cacctgatct tttaacatac tctcctctcc cccggttaaa
     6121 ctggtcggcg tcagaagaga agtcgaaaca atctttaaat ctactgtttt accagacaaa
     6181 gcctgtgata ataactctgg tctgctatat aatgctgcgc ttactaactc ctcagcttta
     6241 ttacgagcag caacagctct ttcagatgag ttttttttca atccataggc tgatataact
     6301 ccatgtctga tacccgaaaa aatttccttc ccctcatcat ccaccacttt tgaaagccac
     6361 atattcgcga tatgatcact ctctctcgta gaagcacaac aaattccttt tccattatac
     6421 tctttgacaa atatattttt attccctatc ttcatatgag aggcaggagt taattgaaaa
     6481 ccataatttt tcccatgatg actaatattc ttgtcgaccg gtccccaatt cttattattt
     6541 aaataatcag taaatatttg ttttattttc gtttgcaatt cctttttatt tttaatgcct
     6601 agctgtgctc ttatctcttc acttattact ttattaagct cattaatatg tcttttatta
     6661 acttcaccaa gtttataggt atctaatcgt tccatcatgg ctttaatttt tgacgtatca
     6721 ccacctgcca actttattgc ctcgcaaaaa acttcagctc tgtcacgcgc aaacaaaagg
     6781 ctttttcctg ttaagcttgt ttggttctgt aaatataatc gattaagcgc ttcgatcccc
     6841 ttatttcttt ctccattttt aactctttga tatgaaatca cgcttacacc tttgtgcttt
     6901 ccggtttctt ctgcgccttt ataggaatct ccgctttgaa atgaaacctg atgcaatccc
     6961 aaattagtta tgtgcattta attatcctca gtcataaatg gggcattgat tgcagttttg
     7021 tacctcgttt tacaacaaaa aagatactta ttcaacaggg ttctctcttt ttctataaca
     7081 ctccgagcct gatacatcag gcactttatc ttgtgggatt tcatgatgaa acgagcacta
     7141 catatgctca cgaggtacat attatgtaca gcaaaagaaa atttgattat gttaacaggc
     7201 tcaagtttct aaatttatta attgaatata ttatatacca aatacatttt acatccccac
     7261 aatcaccaag taatggagag ttaataaagt atgatcctca aaattagcaa tttcattgac
     7321 gcaagcaata caaaagggcc tatacgcgtt gaagatacag agcatggacc tatattgata
     7381 gcacagaaat tcaacctaaa ggatctcttt ttcagaacat taagcactat taatgcaaag
     7441 ataaattcac agatattaaa tgaacaacta aaaaattatc gactagagaa tcaaaaatcc
     7501 ttattgcttt tcctcaatac attagctagc gaaaaatctg cagaaagtgc gtttgccgcc
     7561 tatgaagcag ctaaaaactc aattcaacac tctttcacag gtagagacat caaattaatg
     7621 ttgaataccg cagagcgttt tcatggcatc ggtacagcca aaaatcttga aagacattta
     7681 gtttttcgtt gctggggaaa tagaggcata acccatttag ggcatactag tatcagtata
     7741 aaaaacaatt tactacagga accaactcat acctatctca gctggtatcc cggcgggaat
     7801 gttacaaaag atacagaaat aaactacctt tttgaaaaac gctcaggtta cagtgtcgat
     7861 acctataagc aagacaaact aaatatgatt tcagaccaaa cagccgaaag acttgatgca
     7921 ggccaagagg ttagaaactt attaaattca aaacaagatc aaaataacaa caaaaaaata
     7981 ttttttccca gagctaatca aaaaaaagac ccctatggtt attggggggt cagtgctgat
     8041 aaagtataca ttccattatc aggtgataat aaaacaaagg atggtaaaat cagccataac
     8101 ctttttggcc ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa agcagatgct
     8161 ttcagacaat tggcaaatta taaattaatc agtaaatctg aaaactgtgc tggtatggca
     8221 cttaatgttc ttaaagcagg aaattctgaa atatactttc cactccctga cgttaaattg
     8281 gttgctacac ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg cattgaaagt
     8341 ctcaatcagt cttataacga aataatgaag tatattgaat ctgattttga tctttccaga
     8401 ttgactcaat tacgacgcag ctatcttaaa agtttcaata aaattaacct tatccacaca
     8461 ccaaaaacat tcaagccttt atcaatttca ctttacaaac atcctactga aaatgtatct
     8521 tcagaagact ttgatgcggt catcaacgct tgccactcat acttagtaaa atcagcaccc
     8581 agcaatatga ctcgtgtatt aaacgaatta aaaaccgaag caacagataa aaaagaagaa
     8641 atcatcgaaa aatcaataaa aattattgat tattacaatt ctcttaaatc gccagatctt
     8701 ggaaccaaac tttacattca cgacttatta cagatcaata agcttttatt gaataactct
     8761 cattctaata tatagaaggc catagaaatg tgtcgcaaac tatatgataa actttatgaa
     8821 ataacaggtg caaagcttga ttttaacgat aaaaatcaag cctttatatt gctcgaagaa
     8881 caaatacctg tttgtataac agataatgat gaatacatct ttttgaccgg acttcttaat
     8941 gaacatgaac tttttacaga aaatattatt aatccagaac acatactcat ccttaactac
     9001 tctttgagta gagattatgg ttcttctatc tgtcttctcc ctgatactca tcaatgtgtt
     9061 ttgaccaaga aacactacaa aaaatatctt tcacctgatg aactcattga atctttgtat
     9121 gaatttttat tttgtattaa actaaccatt gcaaacatca cttcagaagt gaactaacat
     9181 atagggggta tcatgcaaat tctaaacaaa atacttccac aggttgaatt cgcgattcct
     9241 cgcccatcat ttgattcgtt aagtcgcaat aagcttgtaa agaaaatatt atctgttttt
     9301 aacttaaaac aacgctttcc tcagaagaat tttggatgcc cggtgaatat aaataaaatc
     9361 agggacagcg tcatagacaa aataaaagat agtaattcgg ggaatcaact attttgttgg
     9421 atgagccaag agcgaaccac ttatgtctct tcaatgataa acagaagtat agatgaaatg
     9481 gccatacaca atggagttgt tttgacctca gataacaaaa aaaacatatt tgcagctatt
     9541 gaaaaaaaat tcccggatat aaaacttgat gaaaaatcag cacaaacatc tatcagtcat
     9601 acagcactaa acgagattgc ctcatctggc ctcagagcaa agattttaaa acgctatagc
     9661 agtgacatgg atttgtttaa cacacaaatg aaggatctta caaatctagt atccagttcc
     9721 gtctacgata aaatatttaa tgaatcaaca aaagttttac aaatagaaat aagcgcagag
     9781 gttctaaagg ctgtataccg tcaaagcaat acaaattaaa tcttttatat taaatcttat
     9841 acttattaaa gtaaagcaga caaaaaggag accttatgtc tttaaatatc accgaaaatg
     9901 aaagcatctc tactgcagta attgatgcaa ttaactctgg cgctacactg aaagatatta
     9961 atgcaattcc tgatgatatg atggatgaca tttattcata tgcttatgac ttttacaaca
    10021 aaggaagaat agaggaagct gaagttttct tcaggttttt atgtatatac gacttttaca
    10081 atgtagacta cattatggga ctcgcagcta tttatcagat aaaagaacag ttccaacaag
    10141 cagcagacct ttatgctgtc gcttttgcat taggaaaaaa tgactataca ccagtattcc
    10201 atactggaca atgccagctt cggttgaaag cccccttaaa agctaaagag tgcttcgaac
    10261 tcgtaattca acacagcaat gatgaaaaat taaaaataaa agcacaatca tacttggacg
    10321 caattcagga tatcaaggag taattattat gcataatgta agcaccacaa ccactggttt
    10381 tcctcttgcc aaaatattgg cttccactga gcttggagac aatactatcc aagctgcaaa
    10441 tgatgcagct aacaaattat tttctcttac aattgctgat cttactgcta accaaaatat
    10501 taatacaact aatgcacact caacttcaaa tatattaatc cctgaactta aagcaccaaa
    10561 gtcattaaat gcaagttccc aactaacgct tttaattgga aaccttattc aaatactcgg
    10621 tgaaaaatct ttaactgcat taacaaataa aattactgct tggaagtccc agcaacaggc
    10681 aagacagcaa aaaaacctag aattctccga taaaattaac actcttctat ctgaaactga
    10741 aggactaacc agagactatg aaaaacaaat taataaacta aaaaacgcag attctaaaat
    10801 aaaagaccta gaaaataaaa ttaaccaaat tcaaacaaga ttatccgaac tcgacccaga
    10861 gtcaccagaa aagaaaaaat taagccggga agaaatacaa ctcactatca aaaaagacgc
    10921 agcagttaaa gacaggacat tgattgagca gaaaaccctg tcaattcata gcaaacttac
    10981 agataaatca atgcaactcg aaaaagaaat agactctttt tctgcatttt caaacacagc
    11041 atctgctgaa cagctatcaa cccagcagaa atcattaacc ggacttgcca gtgttactca
    11101 attgatggca acctttattc aactagttgg aaaaaataat gaagaatctt taaaaaatga
    11161 tctggctcta ttccagtctc tccaagaatc aagaaaaact gaaatggaga gaaaatctga
    11221 tgagtatgct gctgaagtac gtaaagcaga agaactcaac agagtaatgg gttgtgttgg
    11281 gaaaatactt ggggcacttt taactatcgt tagtgttgtt gcagcagctt tttctggagg
    11341 agcctctcta gcactggcag ctgttggttt agctcttatg gttacggatg ctatagtaca
    11401 agcagcgacc ggcaattcct tcatggaaca agccctgaat ccgatcatga aagcagtcat
    11461 tgaaccctta atcaaactcc tttcagatgc atttacaaaa atgctcgaag gcttgggcgt
    11521 cgactcgaaa aaagccaaaa tgattggctc tattctgggg gcaatcgcag gcgctcttgt
    11581 cctagttgca gcagtcgttc tcgtagccac tgttggtaaa caggcagcag caaaacttgc
    11641 agaaaatatt ggcaaaataa taggtaaaac cctcacagac cttataccaa agtttctcaa
    11701 gaatttttct tctcaactgg acgatttaat cactaatgct gttgccagat taaataaatt
    11761 tcttggtgca gcgggtgatg aagtaatatc caaacaaatt atttccaccc atttaaacca
    11821 agcagtttta ttaggagaaa gtgttaactc tgccacacaa gcgggaggaa gtgtcgcttc
    11881 tgctgttttc cagaacagcg cgtcgacaaa tctagcagac ctgacattat cgaaatatca
    11941 agttgaacaa ctgtcaaaat atatcagtga agcaatagaa aaattcggcc aattgcagga
    12001 agtaattgca gatctattag cctcaatgtc caactctcag gctaatagaa ctgatgttgc
    12061 aaaagcaatt ttgcaacaaa ctactgcttg atacaaataa ggagaatgtt atggaaattc
    12121 aaaacacaaa accaacccag attttatata cagatatatc cacaaaacaa actcaaagtt
    12181 cttccgaaac acaaaaatca caaaattatc agcagattgc agcgcatatt ccacttaatg
    12241 tcggtaaaaa tcccgtatta acaaccacat taaatgatga tcaactttta aagttatcag
    12301 agcaggttca gcatgattca gaaatcattg ctcgccttac tgacaaaaag atgaaagatc
    12361 tttcagagat gagtcacacc cttactccag agaacactct ggatatttcc agtctttctt
    12421 ctaatgctgt ttctttaatt attagtgtag ccgttctact ttctgctctc cgcactgcag
    12481 aaactaaatt gggctctcaa ttgtcattga ttgcgttcga tgctacaaaa tcagctgcag
    12541 agaacattgt tcggcaaggc ctggcagccc tatcatcaag cattactgga gcagtcacac
    12601 aagtaggtat aacgggtatc ggtgccaaaa aaacgcattc agggattagc gaccaaaaag
    12661 gagccttaag aaagaacctt gccactgctc aatctcttga aaaagagctt gcaggttcta
    12721 aattagggtt aaataaacaa atagatacaa atatcacctc accacaaact aactctagca
    12781 caaaattttt aggtaaaaat aaactggcgc cagataatat atccctgtca actgaacata
    12841 aaacttctct tagttctccc gatatttctt tgcaggataa aattgacacc cagagaagaa
    12901 cttacgagct caataccctt tctgcgcagc aaaaacaaaa cattggccgt gcaacaatgg
    12961 aaacatcagc cgttgctggt aatatatcca catcaggagg gcgttatgca tctgctcttg
    13021 aagaagaaga acaactaatc agtcaggcca gcagtaaaca agcagaggaa gcatcccaag
    13081 tatctaaaga agcatcccaa gcgacaaatc aattaataca aaaattattg aatataattg
    13141 acagcatcaa ccaatcaaag aattcgacag ccagtcagat tgctggtaac attcgagctt
    13201 aatatatcca agagccataa taatatatgg ctcttcctgt aaggaaataa ccatgaatat
    13261 aacaactctg actaatagta tttccacctc atcattcagt ccaaacaata ccaacggttc
    13321 atcaaccgaa acagttaatt ctgatataaa aacaacgacc agttctcatc ctgtaagttc
    13381 ccttactatg ctcaacgaca cccttcataa tatcagaaca acaaatcagg cattaaagaa
    13441 agagctttca caaaaaacgt tgactaaaac atcgctagaa gaaatagcat tacattcatc
    13501 tcagattagc atggatgtaa ataaatccgc tcaactatta gatattcttt ccaggcacga
    13561 atatccaatt aataaagacg caagagaatt attacattca gctccgaaag aagccgagct
    13621 tgatggagat caaatgatat ctcatagaga actgtgggct aaaattgcaa actccatcaa
    13681 tgatattaat gaacagtatc tgaaagtata tgaacatgcc gttagttcat atactcaaat
    13741 gtatcaagat tttagcgctg ttctttccag tcttgccggc tggatctctc ccggaggtaa
    13801 cgacggaaac tccgtgaaat tacaagtcaa ctcgcttaaa aaggcattgg aagaactcaa
    13861 ggaaaaatat aaagataaac cgctatatcc agcaaataat actgttagtc aggaacaagc
    13921 aaataaatgg cttacagaat taggtggaac aatcggcaag gtatctcaaa aaaacggggg
    13981 atatgttgtc agtataaaca tgaccccaat agacaatatg ttaaaaagct tagataatct
    14041 aggtggaaat ggcgaggttg tgctagataa tgcaaaatat caggcatgga atgccggatt
    14101 ctctgccgaa gatgaaacaa tgaaaaataa tcttcaaact ttagttcaaa aatacagtaa
    14161 tgccaatagt atttttgata atttagtaaa ggttttgagt agtacaataa gctcatgtac
    14221 agatacagat aaactttttc tccatttctg aggtgcgcca tgcataatgt aaataatact
    14281 caagcgccaa cattcttata taaggcaact tcaccatcat caacagaata cagcgagtta
    14341 aaaagcaaaa tatccgatat ccatagttcg caaacttctc taaaaacacc agcatcagtg
    14401 tctgaaaaag aaaactttgc aacgtctttt aatcagaaat gtcttgattt tttattttct
    14461 tcctcaggga aagaagatgt gttaagaagc atttattcca actcaatgaa tgcgtatgcc
    14521 aaaagcgaga ttctcgaatt ttcaaatgtt ttgtactcct tagtacatca aaatggtctt
    14581 aattttgaaa acgaaaaggg acttcaaaaa attgtcgcac agtattcgga actaattata
    14641 aaagataaat tatcccaaga ttctgccttt ggaccatggt cggcaaagaa taagaaactc
    14701 catcaattac gacaaaacat tgagcacaga cttgcactat tagcacaaca acacacatct
    14761 ggtgaagctt tatcattggg acaaaaactc ctcaatactg aagtatcatc atttatcaag
    14821 aataatattc ttgctgaatt aaagttaagt aatgaaactg tttcatctct caaactagat
    14881 gatttagttg acgcacaggc aaaacttgcc tttgatagtt tgcgcaatca acgtaaaaat
    14941 actattgata gtaaaggatt tggtataggt aaactgtcaa gagacttaaa tacagtagcc
    15001 gtgtttcctg agctgttgag aaaagtcctt aatgatattt tagaagatat aaaagattcg
    15061 tatcctatcc aagatggcct ccctacacct cccgaagata tgccagatgg cggaccaacc
    15121 cccggagcca atgagaaaac atcccaacct gtaattcact atcatataaa taatgataat
    15181 agaacttacg ataatagagt ttttgacaac agagtatatg acaatagcta tcacgagaac
    15241 ccagaaaatg atgcacagtc tcctacttct cagacaaacg atctattatc ccgtaacgga
    15301 aactcattac taaatccaca aagagcacta gttcaaaaag taacttccgt tctaccacac
    15361 tctatatcag atactgtcca gacatttgca aataattcag ctttagaaaa ggctttcaac
    15421 catactccag ataattcgga tggaataggt tcagacctgt taactacgag tagtcaagaa
    15481 agatctgcaa ataactctct ttctcgggga cacaggcctc tgaacataca gaactcttca
    15541 accacccccc ctctccaccc ggaaggagtg acaagcagta atgataactc atcagataca
    15601 actaaaagta gcgcttctct ttctcataga gtagcttcgc aaatcaataa attcaactca
    15661 aacactgatt caaaagtact tcagactgat tttttatcaa gaaatggaga cacatattta
    15721 acacgggaaa cgatatttga agcttcaaaa aaagtaacaa actccctaag taatcttata
    15781 tctctcattg gaactaaatc aggaacacaa gaacgagagt tacaggaaaa atcaaaggac
    15841 attacaaaat ccacaacaga acatagaata aacaacaaat taaaagttac agatgcaaat
    15901 ataagaaact acgtaacaga aaccaacgca gatacaattg ataaaaatca tgcgatctat
    15961 gaaaaggcaa aagaagtatc tagcgccctc agcaaggtat tgtcaaaaat tgacgatacc
    16021 tctgcagaat tacttacaga tgatatatct gatttaaaaa ataacaatga tattacagct
    16081 gaaaacaata atatatataa agcagcaaaa gatgtaacca cttccctatc aaaagtatta
    16141 aagaatatca ataaggatta aaatatgata aaagaaaaaa tattatcaat agtggccttt
    16201 tgctatggta tagcgtacag caaactatct gaagaaacta aatttattga agatctttct
    16261 gccgattctc tttctctgat tgaaatgctg gatatgatta gttttgagtt caatctacgt
    16321 atagatgaat ctacattaga acacattatt actattggag atcttatttc tgtagtcaaa
    16381 aatagtacaa aatcaatata gtaattgata agcatttttt catctatgga gctctcacat
    16441 cagagctcca caagaatatt attcttttat ccaataaaga taaattgcat caatccagct
    16501 attaaaatag taatttttaa gactaccgtt gactatcatc aacagttaca tacaatcttg
    16561 ctcacactgc atttaacttt tgtcaatata aatcaatatg aataaacagg gtgtgatatg
    16621 gtggatttgt gcaacgactt gttaagtata aaggaaggcc aaaagaaaga gtttacactc
    16681 cattctggta ataaagtttc ctttatcaaa gccaagattc ctcataaaag gatccaagat
    16741 ttaaccttcg tcaaccaaaa aacgaatgta cgcgatcaag aatccctaac agaagaatca
    16801 ttagccgata tcataaaaac tataaagcta caacaattct tccctgtaat aggaagggag
    16861 attgatggta gaattgaaat tctggatggc actcgtagaa gagcatctgc aatatatgca
    16921 ggagcagatc ttgaagttct atattcaaaa gaatatatat ctactcttga tgccagaaaa
    16981 ctagcaaacg atatacaaac agcaaaagag catagcatcc gagaacttgg tattggtctt
    17041 aattttctga aagtatcagg gatgtcctat aaagacatag ccaaaaaaga gaatctgtct
    17101 cgcgcgaaag tcactcgtgc ctttcaggca gcaagcgttc cacaggaaat aatatctcta
    17161 tttccaatcg cgtcagaact taactttaat gactacaaga tattattcaa ttattataaa
    17221 ggacttgaaa aggctaatga atctcttagt tctacactac caatattaaa ggaagaaata
    17281 aaagaccttg atacaaattt gcccccggac atatataaaa aagaaatttt aaacatcata
    17341 aagaaaagca aaaacagaaa gcaaaaccct tcgctaaaag ttgactcgtt atttatttct
    17401 aaagacaaac ggacttacat aaaaagaaaa gagaataaaa caaacagaac tctaatattt
    17461 acattatcta aaataaacaa aacagttcag agagaaatag atgaagctat tcgggatata
    17521 atttctcgcc atctatcgtc ttcataaaca tcgaacactg atgtaaactg cccaattcag
    17581 ataataaaaa tcttcgttga acttgacttt cagccggtgg ggtaattccg tcggctatct
    17641 gttattcaat ccccacgcac tgtgtttcct taaaattacg gtgaaaactc ataccctgct
    17701 gaccacgaga aaattatctg gtgaaattag taatattcat atcattgaaa atgaattgtg
    17761 caactaaggg atgcggcatg atggtcaaaa ttttatgtga atatgagtga ctggccgaaa
    17821 atattcagcg tatttttaat cgtttcaata aaaacagcga ggattagcag tcaacgcttt
    17881 tactactcaa tttttttaca tggtgataaa caaaaaatcc accattaaca gacttcgttc
    17941 acagtatcaa aaaagcttta agaatatatt agcagatttc tcaaatataa ttactgtttt
    18001 attctcctaa tcttcccata aaatgaccga cttacattat tttataatta taaacaagtt
    18061 tgattttaaa atcatattaa ttattaacaa gagatttatt tttctaaccc caaaagttat
    18121 gcttggtcaa agagtaactt ataatatcca tacccctcat agggcttaca gtttaaattg
    18181 cgggtataaa atacatctat tttcaaataa aataaagcat actccaatta caacgagtga
    18241 ctaaaataaa aatatcatcc aagtcaatca ctgtttgctc tgcaaaatat gtcagccttc
    18301 tcaaacttac cagcacttaa atatttaagc acacaatcat tccttgaggg agttcactta
    18361 tttcaagaga aacatttcgt cttcccacac aactaatccg cccaatatag ctaaatttgt
    18421 actcagtaat atcatttgtt atgcccccca ccccaaaata ttcaagctcc ttcacagata
    18481 aaaaacgaca atttacaaag cctcattagt tataactatg gaaataccta ttttagtata
    18541 tcctataaag ttagaattac ttcttcttgc acctaattcc atagttgaaa aacaagaata
    18601 attttctcct acagctggat ccatatagct tccatcaggt ctacacaaca cccagtggag
    18661 gccgacagga actccaataa aggatacagc aacagcttcc aaaactcttt ggttagaaga
    18721 taacacatct cgttgatgta ctatattaca tcccatgcca attaataagt cccttgcatc
    18781 gggataaata aaactaatta cttgcgaaaa aatgttactt tcttcaacga cataagcatt
    18841 caatccaaga agcctcgtag ccatgactat cttatcaggc atagaataac cggcatccgc
    18901 gatattatct ttgtgtattc tcggattata attaccacta gttattaagt ataaatcccg
    18961 ctcacaacga ttatccaaat caagagaact cttccttgtc atttcggaca tagacccttt
    19021 gtattgtggt attttatcaa cccctaattc ctttgcagca cagagtaaac tggcagctcc
    19081 acaagaattg tcatacatct gcttcctcgc caaaaaacaa ccttgttcgt ttactctatt
    19141 ctcatttacc ctcacatcag tctgatgaga tatcatatat tctcttggta tagccccctg
    19201 caataaaacc ccatataaca taacacctgt atgaatacat ccccgtttgc aaggcttccg
    19261 ttgttccgac atcgacgagt aattgcagca cagtattgca tccagcaatg cgaactacta
    19321 tgagaattag ctgaccgggt ccttattccc cgaacgaact ttaaatccaa aacctcatac
    19381 tcttttccgt cgtcatcacg tccgtcacca taacaacctt tcagcatcca cccttttatt
    19441 aagagttaat gtccatcagc atcagcaatt aataccgcct gatactgaca tatttttgtc
    19501 gtatttacgt ccataatcgg ttattgtgat acaggtcaca ccagcttatt tgctattgtt
    19561 acaataacca ccttggttga gagctgtttt ttcaactttg ttatcaactc ccccaactcc
    19621 ttggtcagta aggctgccga ccgagcacag tgaatagata gtatccgcag gtaccaccat
    19681 gcttacttaa tactcagaag gcgaagtgct ctccaattaa ctctgccgca ctatgcttat
    19741 aagctgcttt tcaatgccgt ctatcaacat gtccaattca ttcaatctgt tgtactggta
    19801 atccaacgca gtaatgatat atgcaggtat tctttcatcc actccagtaa cgtttcatgc
    19861 aaagcattaa tttacgcgct acggaacttc accagttgac tgagcaaacg atgtgggacc
    19921 agtactgcct gcagttcttc tattgttaca gcgatttctt taccaaacag ctgaaccacc
    19981 atcaagataa agatagcctg aacattcatc gcaccatttt gttaccaatg gcaaaagatt
    20041 taacgaaccg tgcctgcatc aggcggactt tttatccctg ctttgttagc tccctaacct
    20101 tgtacagaga gcctccataa gctcccatat caagcagaca tggttcgcag ttactgacat
    20161 acaccagaaa actctgacag cagactcact tatcctccac ttcactcata tccttattgg
    20221 tgagatgaac ctgaatgaca tgttttgcga cataaacgcc aaccagtgta tatttcacct
    20281 gtagagattc tacaatctgt agcgttatgc atctaatgtt aggctctatg atgtcagaaa
    20341 tctgcgagac tctacacctt ctgcatttac ctttatttat caccatgctc ctcagttaac
    20401 ggagaacgtg ttcattgcat tttgtcactt acatttcgcc tttggtatcg gggggatata
    20461 ggttccaacg ttccgcatga gaacctgaac cggcttcacg ccaccttgat accagatgcc
    20521 gcacaggtag tcaacaagtg tcacgaacgg tgcaatagtg atccacaccc aacgcctgaa
    20581 atcagatcca gggggtaatc tgctctcctg attcaggaga gcttatggtc acttttgaga
    20641 cagttatgga aattaaaatc ctgcacaagc agggaatgag tagccggacg attgccagag
    20701 aactggggct ctcccgcaat accgttaaac gttatttgca ggcaaaatct gagccgccaa
    20761 aatatacgcc gcgacctgct gttgcttcac tcctggatga ataccgggat tatattcgtc
    20821 aacgcatcgc cgatgctcat ccttacaaaa tcccggcaac ggtaatcgct cgtgagatca
    20881 gagaccaggg atatcgtggc ggaatgacca ttctcagggc attcattcgt tctctctcgg
    20941 ttcctcagga gcaggagcct gccgttcggt tcgaaactga acccggacga cagatgcagg
    21001 ttgactgggg cactatgc
//
LOCUS       DAJTRW010000066        20900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRW010000066 DAJTRW010000000
VERSION     DAJTRW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..79)
                     /locus_tag="OO326_002664"
     CDS             complement(<1..79)
                     /locus_tag="OO326_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR5967005.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIM"
     gene            complement(134..985)
                     /gene="manZ"
                     /locus_tag="OO326_002665"
     CDS             complement(134..985)
                     /gene="manZ"
                     /locus_tag="OO326_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5967006.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(998..1798)
                     /gene="manY"
                     /locus_tag="OO326_002666"
     CDS             complement(998..1798)
                     /gene="manY"
                     /locus_tag="OO326_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5967007.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1861..2832)
                     /gene="manX"
                     /locus_tag="OO326_002667"
     CDS             complement(1861..2832)
                     /gene="manX"
                     /locus_tag="OO326_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5967008.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3295..4845
                     /gene="yoaE"
                     /locus_tag="OO326_002668"
     CDS             3295..4845
                     /gene="yoaE"
                     /locus_tag="OO326_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5967009.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4849..6447)
                     /locus_tag="OO326_002669"
     CDS             complement(4849..6447)
                     /locus_tag="OO326_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5967010.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEHNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6578..7942)
                     /gene="sdaA"
                     /locus_tag="OO326_002670"
     CDS             complement(6578..7942)
                     /gene="sdaA"
                     /locus_tag="OO326_002670"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5967011.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8126..8704)
                     /locus_tag="OO326_002671"
     CDS             complement(8126..8704)
                     /locus_tag="OO326_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5967012.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8708..10069)
                     /gene="pabB"
                     /locus_tag="OO326_002672"
     CDS             complement(8708..10069)
                     /gene="pabB"
                     /locus_tag="OO326_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5967013.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10143..10322
                     /locus_tag="OO326_002673"
     CDS             10143..10322
                     /locus_tag="OO326_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5967014.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10442..10801)
                     /locus_tag="OO326_002674"
     CDS             complement(10442..10801)
                     /locus_tag="OO326_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5967015.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11163..11507)
                     /locus_tag="OO326_002675"
     CDS             complement(11163..11507)
                     /locus_tag="OO326_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5967016.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11639..13549
                     /locus_tag="OO326_002676"
     CDS             11639..13549
                     /locus_tag="OO326_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5967017.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13607..14302
                     /gene="tsaB"
                     /locus_tag="OO326_002677"
     CDS             13607..14302
                     /gene="tsaB"
                     /locus_tag="OO326_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5967018.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14342..14923
                     /gene="yeaY"
                     /locus_tag="OO326_002678"
     CDS             14342..14923
                     /gene="yeaY"
                     /locus_tag="OO326_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5967019.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15129..16814
                     /gene="fadD"
                     /locus_tag="OO326_002679"
     CDS             15129..16814
                     /gene="fadD"
                     /locus_tag="OO326_002679"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5967020.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16896..18011
                     /gene="rnd"
                     /locus_tag="OO326_002680"
     CDS             16896..18011
                     /gene="rnd"
                     /locus_tag="OO326_002680"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5967021.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18065..19030)
                     /gene="yeaX"
                     /locus_tag="OO326_002681"
     CDS             complement(18065..19030)
                     /gene="yeaX"
                     /locus_tag="OO326_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5967022.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19086..19703)
                     /gene="yeaW"
                     /locus_tag="OO326_002682"
                     /pseudo
     CDS             complement(19086..19703)
                     /gene="yeaW"
                     /locus_tag="OO326_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20045..20611)
                     /locus_tag="OO326_002683"
     CDS             complement(20045..20611)
                     /locus_tag="OO326_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5967023.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT         5152 a         5189 c         5383 g         5176 t
ORIGIN      
        1 gcatgatgaa ctgatcgtag atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta
       61 ccaggtccgt gattgtcatt aatcctccag ataaaaaaac ggggccaaaa ggccccggta
      121 gtgtacgaca gtcttacagt cccagcaggc cgcaagcgta accagcgata ccgatgacga
      181 agaagccaac gatgatccac agcgggttaa cttttttgcg cagcagccac atacaagcaa
      241 atgtcagcag cagtggtaca aggcctggca ttaactggtc aagaatagtt tgcacggtgg
      301 tgacacgggt ctgtccatcc tgcccggtga tggtcgaaac caccagcggg atgttgacat
      361 gtgtccactt gttaaccaat gcccccatga caaacaggcc gaggatagac gccccttccg
      421 tcagtttttg caggaagcca ccacccatat ctttaacgat atcgatacct ttggagtaac
      481 catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacagg atgaagaaca
      541 gcagcggacc taacaggctg ccgctcatcg cgataccggc acccagtgct gcaaataccg
      601 gacgtacggt tccccagaag atcgggtcgc ctacaccagc cagcggcccc atcaaaccga
      661 ctttgatacc gttgatagca ccgtcatcga tctctgcgcc attagcacgc tgttcttcca
      721 gcgccagggt tacgccgaga atcggtgcag ccacgaacgg ctgggtgtta aagaactcca
      781 ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg cgacgaattg
      841 ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc catgaaccct
      901 ggaagaggtt agaacgcagg aatacgccac gaatatcact ttgagtgagt tttttctcgg
      961 tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg ttatcgagat
     1021 cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc gggctaagtt
     1081 ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg ttaaagttgg
     1141 tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca gcacgcatca
     1201 tgttgataac catcgcataa ccaaccacca cgatcatgcc cccggcgata ttcagaccat
     1261 tggtcaccac ttccggaatc gcattcagca tgttctgtac ttcgctggta ccaacagaca
     1321 gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga gaagaaacgt
     1381 ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca gcgtgctgga
     1441 aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg gccagaggga
     1501 ttgccagtgc gatacctgca ccaatgctct gatgacctgc gataaccaga atggtagaaa
     1561 tgatagaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg
     1621 cgatcatttc cagcgtacca ccgataataa taccggtttt catatcccca agaacgatac
     1681 ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg attgatccca
     1741 tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta atctccattg
     1801 tacttctcct gtttacgtaa gacttaagtg tgaaaactga atgataatca acacaatacg
     1861 ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg tggaaacctt
     1921 acggacttcc agctcaatac cgcgtgcatt cagttttttg aacgcctcga tatctttttc
     1981 atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca taccaccgac
     2041 gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg ttgggttggt
     2101 aaataacagc attacgcgtt cgccagcata tttcgggttg ttgtagacgc gaatcatttt
     2161 ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga gcagtgtctt
     2221 acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga cattggtttc
     2281 tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc
     2341 aataaccatg tagtcgtttg gccccattgg ttttgccgga gttggagccg cttttggtgc
     2401 tgcggcagcg ggtgctggcg cggctttttc aaccggtttg gctttcagtg ctttcacgcc
     2461 ttcacggcct gtttctactg ccagcgcaac cagttcatca aagctcgggt catcatcacg
     2521 ggccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt cataatgctc
     2581 tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg tatcaacgag
     2641 aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtatt tttcaatcag
     2701 cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct gctcgcctaa
     2761 cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat
     2821 agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc cagatgtatt
     2881 tttgaatcgt cgtccacaat cgaatcgatt cagataaggg ctacaaccaa aaacccctgc
     2941 gtttcgcgat ttattttaga tatcgaaaaa attattttat gtgatgaaga tccgtaattt
     3001 aactttcgat tatcagaaat ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag
     3061 gaaggtaaat ctttgccaaa tcagaggcgt ctctgatatg tttaactccc gtttaacaac
     3121 catggagtat agggcagtag cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag
     3181 gccttttaac gcctgccttt ctggcactct atgccgcacc tttcgtttgc attttgtcgt
     3241 tacgcctgca ttatttctgg cgtcgaatag ctattcctta agcaggagct tgtcatggaa
     3301 ttcttaatgg acccctcaat ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg
     3361 ctgggtatcg ataacctggt cttcatcgcc attcttgctg acaaactgcc gccaaaacaa
     3421 cgcgataaag cgcgtttgct ggggttatca ctggcgctga ttatgcgtct ggggctgctg
     3481 tcgctgattt catggatggt cacgctgacc aaaccgctat ttaccgtcat ggatttctcc
     3541 ttctccggac gcgatctgat tatgttgttc ggggggatat tcttgctgtt caaagcaaca
     3601 accgaactgc atgaacggct ggaaaaccgc gatcatgatt ccggccacgg taaaggctac
     3661 gccagtttct gggtggtcgt cacacagatt gtcatccttg acgccgtctt ctcgttggat
     3721 gcggtaatta ctgcagtagg gatggttaac catctgccgg tgatgatggc ggcggtagtg
     3781 attgcgatgg cggttatgtt gctggcatcg aaaccgctga cgcgattcgt taaccagcac
     3841 cccacggtgg tggtgctctg tctgagcttc ctgttaatga ttggtctgag tctggtggca
     3901 gaaggtttcg gtttccacat tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc
     3961 attatcgaag tgtttaacca gattgcgcgt cgcaacttta ttcgtcacca gtcgactttg
     4021 ccgctgcgag cgcgtactgc cgatgccatc ctgcgtttga tgggcgggaa acgtcaggcc
     4081 aatgttcagc acgatgccga taacccgatg ccgatcccgg aaggtgcatt tgccgaagaa
     4141 gaacggtaca tgattaacgg cgtactgacg ctggcgtcgc gttctctgcg cgggatcatg
     4201 acgccgcgcg gtgaaataag ctgggttgac gctaatctcg gggtcgatga aatccgcgag
     4261 caactgctct cttcaccgca cagtctgttc ccggtatgtc gcggtgaact ggatgaaatc
     4321 atcggtatcg tacgtgctaa agaactgctg gtggcgctgg aagagggcgt tgatgtggcg
     4381 gcgattgctt cggcgtctcc ggcgattatc gtcccggaaa ccctcgatcc gatcaacctg
     4441 ctgggcgtgc tgcgtcgcgc tcgcgggagc tttgttatcg tgaccaacga gtttggtgtg
     4501 gtacaaggct tggtcacgcc gctggatgtg ctggaagcca ttgcgggtga attcccggac
     4561 gctgacgaaa cgccggaaat cattaccgat ggtaacggct ggctggtaaa aggcggtaca
     4621 gatttgcatg ccttgcagca ggcgcttgat gttgagcacc ttgccgatga cgatgatatc
     4681 gcgacggtcg cgggcctcgt gatctcggca aatggtcaca ttccccgtgt gggcgatgtg
     4741 attgatgtag ggccactgca tatcaccatc tttgaagcca atgattatcg tgttgatctg
     4801 gttcgcattg ttaaagagca accggcgcac gatgaagatg agtaagcgtt aacgtaacga
     4861 cataatgggc gtgatatgtc cattatgccg ggcgggcggc ggttggctgc ccgccagcca
     4921 tttgggaaaa tcacgtagcg gcatcggctg tgcgtacaaa tacccttgca aaatatgcac
     4981 gccatgacgg cgcaaatact tcgcctgttc ttgcgtttcc acgccctccg ccaccagttc
     5041 aatattcagt ctttgcccca gcgcgatgat gatatcggtc accgtcgagt taaccgcgtc
     5101 agaacctata gctgcggtaa atgacttatc aattttcagc acgtcaggac gtaatgtttc
     5161 aagccaggaa aacgagctgt tgccggtgcc gaagtcatca atcgccagtt tgacgttttt
     5221 acgatgcagc tcgcgggcaa tccgataatc aacatccagt aaggcatcgc gttcggtgat
     5281 ttcgaggatc agttgctgaa tcgggtgagc gctaaaccag tactgattga gatcttttat
     5341 cagcacacca cggcgaaaat ggctgggtgc gacgttaatg ccaacatgaa actgactact
     5401 catcgggaaa acatggtgct gacgaatggt ttctgccatc acatagtggg tcagtggcac
     5461 aattaaatga tgttcttccg cgataggaat aaacacatcc ggtgaaatcc agccctgacg
     5521 cggattattc cagcgcagca aaatctctac accaatacat tgctggctgc gcgcattaag
     5581 caaaggctga cagaacaatt cgaactcatg ttgcgccagg cccagattaa tttcgcggga
     5641 aaagctcatc cggtaagcgg tcgccagcca ggcgatatag cccaccagta aacttagcag
     5701 caccgccagc ggtaattgtg ttggaagata gtgccatgcc agcgccgtag cccccggacc
     5761 atttacgtta atggtaaaag gaaagctttg cgaagaaacc tgatagtttt cattgtgttc
     5821 aggttgcgga agggaatcta ccagcccatt accatagagc aaatgccgtt tgtccaccgt
     5881 caggctggcg ctactgattt gcggcagttg tggctcaagg agcattgccg tcagtaagtc
     5941 gatgttaatc atctctatga ccccagcatt gctactgccc gctgcaggcg tccattgaat
     6001 caaaaccgga ctgcctttaa tcagggcacg gtcgatcgtc aggcgtaaaa gtggttgcgg
     6061 tgcaggaagt tcagccagaa tgtccacgac gggcacattg cgataaccaa aaatgctgga
     6121 gcaataaagt gtgccgtctt gcaccaggcc aatggatcgc acagtttgga gttttgccgc
     6181 ctgtttacgt aatggcaaat gggcgacaga gcagggcaga ccaatcagcg gaagcaagac
     6241 tttgctacct gcctgtaggg gaagcagtac tttatctaat tcctctacag cgtgattggc
     6301 gaattgtact acccgttgtt gatttaagtt acgctgtgaa ataaatcgca cgctcagggt
     6361 cgaagcgagc gtaacgagtg cgcaaatcgt acaaacaatc attcgattac ggcgataggt
     6421 tttaatgatc cgttgtgctt tttgcatgcg ggccaccctg taagcgtatg gcgacaaacg
     6481 cctgacgcaa cagtggaagt gtagtgggga atattacagc agacgagaaa gcgggtataa
     6541 attcgcccat ccgttgcaga tgggcgagta agaagtatta gtcacactgg actttgattg
     6601 ccagaccacc gcgtgaggtt tcgcggtatt tggcgttcat gtccttaccg gtttcgtaca
     6661 tcgtttcgat gaccttatcc agcgagacgc gcggtgcact ggtgcggcgc agagccatcc
     6721 gtgcggcgtt aatcgccttc acagaggcaa tggcattacg ctcaatgcac ggcacctgaa
     6781 cctgccctgc aaccgggtcg caggttaaac caaggttgtg ttccatgcca atttccgccg
     6841 ccacgcaaac ctgttccggg ctaccgccca gcagttctgc aagacccgca gcagccattg
     6901 aacaggcaac acccacttcg ccctggcaac caacttccgc accggaaata gaggcgttca
     6961 ttttatacaa tgcaccaatc gcgcccgctg ccataaagta acgggtatag atgtctgggc
     7021 tgaccgattc aataaagtgg tcatagtaag ccagcactgc cggaacgata ccgcaggcac
     7081 cgttggttgg cgcagttacc acacgaccac cggcggcgtt ttcttcgtta actgccagcg
     7141 caaacatgtt tacccagtca atgacattca tcggatcgtt agacagttta tcgctggaaa
     7201 ctaacatccg gcgcagggca gacgcacgac gtggcacgcg cagcgggcct ggcagtacac
     7261 cttcggtgtt catcccgcga tcgatacatg cctgcatggt ttgccagaca tgcgcgaaat
     7321 actcgtcgat ctctttcttg ctgtgcagcg ccagttcgtt ctgcatagcg agaccagaca
     7381 gcgaatagcc ggtttcatta cagtacgcga gcagttcggt ggcagatttg aacggatacg
     7441 gcacgctcac ttcgttggca gcatcctgac caaagtgttc ttcatcgacg ataaaaccgc
     7501 cgccgatgga ataataagtt ttgctgtaga cgacttcatc gccgttatag gcgtggattt
     7561 gcataccgtt ttcatgcagc ggcaggttgc cgttatgaaa acgcatcccg ttgtcgcgcg
     7621 ggaaatccac ttcatgccgt ccctgtgcca gcagcagacg ttcgcgctct tttacgtcgc
     7681 gaataaaacc gggaatactg tcgatatcca cggtggcagg ttcgttacct gcaagaccca
     7741 taataatggc gatatcggtg tggtggcctt tacccgtcag cgacagtgaa ccataaacgt
     7801 ccacggcaac gcgagtaacg ctatccagta agcctttttc gaccagatca tcgacgaact
     7861 gtttacccgc cttcataggc cctacggtat gggaagatga gggaccaatc cccaccttaa
     7921 acatgtcgaa tagactaatc acgataatac tcctgacaag gtgactggac ttccagtaac
     7981 gaactaataa cagcgcatag tgtaagaggg aacgcggcga ctggcttaac tattcacatg
     8041 aattaaacta atcaaaaacc gcgatttaac taatattgcg atgattgtct caagtagtgt
     8101 tttgtagatg taaagataag tatagtcagg gggtcacacc aatttgcagc gccagctcac
     8161 gaattatgcc tgcggtcatt ccccatacaa aatactgttc gtaccaggac agccataccc
     8221 gatgtgaatc gccacggcgg tagatatcta aagggtgata acgacccaga tgtaatgcct
     8281 gggcgagcgg catttcaaac accgccgaga cttcatcttc actggcgcga tacggcagat
     8341 cgggcgggat aatgccgacc actggggtta cttgatagcc agttacgcta tcgacgggcg
     8401 gcagcacgcc gataacttca acggcggaag gcggtatagc gacctcttct tcagcttcgc
     8461 gcagcgcggc ggcgataact gatgcgtccg tgtcatcgac tgcacctcca gggaatgcca
     8521 cttgtccagc gtgtttacgc agatgaattg aacgctgagt cagcaacaac cccggttgcg
     8581 gtcgacggac gatggggatt aacacggcag cctgacgatg atttaggggt tcccggttaa
     8641 tttgcgggcg caaaagttga aagcgagata aaaaatcatc aagcgtcagg ctacggtatt
     8701 ccacgtctta cttctccagt tggcgtaata tcttattaac tttatcaaaa gtttcctgat
     8761 attccgcttc ttcctggcta tcggcgacaa ttccaccgcc cgcagagcag tatatttgtc
     8821 cgttaatggc agtcagcgtg cggatagtaa tgctggtatc catgttgccg caaaagctca
     8881 aatagccaat gctgccgcac caggcattac gtcgctgcgg ttccagttcg tcgataattt
     8941 ccatagcccg tactttcgga gccccggtta ttgagccacc aggaaaagct gcgcgcagca
     9001 gatcgctggc gtgtaactgt tctggtagtc gcgccgttat agtgctgacc agatgatgca
     9061 cggcagggaa gggttccacc acgaagagct ctggtacttt taccgaaccg gctacggcaa
     9121 cacgaccgat atcattacgc attaaatcga caatcatcag attttcggca cgatctttcg
     9181 ctgagttcgc cagtttttct gcttgtttgc tatcttcctg aggatcgggc aggcgtggta
     9241 gcgtgccttt aatcgggcga gtctggattt cactattatc acaaagaata aaccgctctg
     9301 gcgaaaggct taaaattgca ccctgttcaa gacgtaaaaa agcgctaaat ggcgcgcggt
     9361 tggcctgatt aagctgaagg aatgcctgcc attcatcgcc agaataggtc gcatgaaaac
     9421 gctgggcgag attcacctga tagcaatcac cgctgtgcag atattcctgt acctggcgaa
     9481 atttttcgcc gtactgctcg tgggtcatat tggattgcca gtcggaagtg agcgtgaaat
     9541 cttcctgcgg cgagaattgc tggctttcca gccaggcccg acgggcattg acatcattat
     9601 gactcagcaa agaaactgta tgacgctggt ggtcgacaat gagcgcccaa tcgtagatac
     9661 ccactgccat atccggcaga acgatatctt gttccgcaat ttctggcagt gactcaaaac
     9721 ggcggcccag atcgtagcca aacaacccca gtgcgccgcc ctgaaatggc aaatcttcgt
     9781 tatgcgttgg gcgaatgtct gcgcgatcca gcacctgctg gagcacctgt agcgggtcat
     9841 cagtggtcgt tgtgcgtttt tcgctttcac taacaacggt ttctttacct aaagtggtta
     9901 aagtgcaaat cggatcggcg accacaatat caaagcggct atacggatga tcggcatagc
     9961 cggagtgtaa aagcatcgcc cacggcaggt ggcttaagcg ggagaaataa aattcagcgg
    10021 cgtcctgacg ccagggtaaa gtaatcacag tgggagataa cgtcttcatc agtcctgact
    10081 ctactggcta tgtgccgggt agcatgaaat aattagcgcc aacaatttag caggagttaa
    10141 caatgtttgc aggtttacct tcactcatcc atgaacagca gcaaaaagct gtcgagcgga
    10201 tccaggaact gatggcacaa gggatgagta gcggccaggc aatcgcgctg gtggcagaag
    10261 agctgcgtgc caaccactcc ggcgagctaa tcgtggcgcg ttttgaggat gaagacgagt
    10321 aacacactaa tccgtatcga tttcgttata gaagccgatg atcatttgat tgtgagaaaa
    10381 aggacgcttt ttatatgtga ttaaaagcgt cctgacccat ttatgctgat ataaatttaa
    10441 ctcagaccag tgcttttagt tcttcctgca tatatgttga aaagtattca ggatttttaa
    10501 tcacagagcg ttcacctgtc tccatttttt ttgcccatcc aaccattttt tccgtgaacc
    10561 cagggttcca gccttcctgg tcagctcgcg ccgtaatatc agcggcactc gcgggtactc
    10621 ccagttcttt taaatattta aagattccct tcgccgtgct ttcattcatc gaacttggtg
    10681 ttggcgctga tacatccatg gcaccaatga aatccagggc tttatctatt acagcaggca
    10741 tattcttatc cttaaaatta accatgttat cgataaggct atatatgacc ttaatttgca
    10801 tctaatatca aagggattat aacgatatgg cacgagttaa cctgttgaca ttcaggtgtt
    10861 ttaaaggtag cagctcatgg tgtgttatcc agtaagatgg agaaaattaa cggtcagccc
    10921 gcacgctata accagattgt tcagctaaga aagaaaacca gagttgctaa tcaaagggcg
    10981 aacacaacgg catggttatg tttatttata tagttagttt tatagataaa attgaaatta
    11041 taaatataaa taatgaatta gcagaagcat caacagtaat atcttattct ctggtcgatt
    11101 tatgtaaaac cgaccagtat tatcgataaa ttatcgtctg acttattttg cttcgataaa
    11161 gcttacaccg cagccacaat cttaatttca actttatact tcgggttcat caaacccgct
    11221 tgtaccgtgc agcgcaccgg cgcatgaccc gcgacaaccc aggcatccca cgctttattc
    11281 atcgccgcga agtcgttttt atcggccagg aaaatggtgg catccagaat gctcgattta
    11341 ttgctgccct gtttttccag cacggcgtca atctgtgcca gcgtgttggc ggtttgctca
    11401 aaggcatcgg cgtcgaggtt ttccggtaca ccagtgtagt agagcgtgtt gttgtggatt
    11461 actacatcag accagcgggc ttcagcatcg atacgaacga tagtcatcat tttgtcctca
    11521 ttatacttcc attttcgtgc tgcaaagact gccacaatgt gaggttgccg tcaccacttc
    11581 aactggcgaa agcgccctca tcctgacata atccctgttc aaatcaacag ggggtagtgt
    11641 gacggacgat tttgcaccag acggtcagct ggcgaaagcg ataccaggct ttaagccgcg
    11701 agaaccacag cgacagatgg cggtagccgt cacccaggcg atagaaaaag gccagccgct
    11761 ggtggtggaa gcaggaaccg gtacgggcaa aacctacgct tacctggctc ctgcgctgcg
    11821 ggcgaaaaag aaagtcatta tctcgaccgg ctcaaaagcg ttgcaggatc agctctacag
    11881 ccgcgatttg ccaacagtct caaaggcatt gaaatatacg ggcaacgtgg cgctgctgaa
    11941 agggcgctca aactacctct gcctcgaacg tctcgaacag caggcgctgg cggggggcga
    12001 tctgccggta caaatcttaa gcgatgtgat cctgctgcgc tcctggtcta atcaaacagt
    12061 cgatggtgat atcagcacct gcgtcagcgt ggcggaagat tcacaggcgt ggccgctggt
    12121 caccagcacc aacgacaact gtcttggcag cgactgcccg atgtataaag attgctttgt
    12181 ggtcaaagca cgtaaaaaag cgatggacgc cgatgtggtg gtggtaaacc atcatctctt
    12241 tctggcggat atggtggtta aagagagtgg atttggcgag ttgatccctg aagcggacgt
    12301 catgatcttc gacgaagccc accagctacc ggacattgcc agccagtatt ttggtcagtc
    12361 actctccagt cgacaactgc tcgacctggc aaaagacatc accatcgcct accgcaccga
    12421 attaaaagac acccagcagt tacaaaagtg cgctgatcgt cttgcccaga gtgcgcagga
    12481 ttttcgtctg caactcggtg agccaggtta tcgcggtaac ctgcgtgagc tgttagctaa
    12541 tccgcaaatt cagcgggcat ttttactgct cgatgacacc ttggaacttt gttatgatgt
    12601 ggcgaaactg tcactggggc gttccgcctt gctggatgcg gcatttgagc gcgccacgtt
    12661 gtatcgcaca cggctgaagc ggctaaaaga gatcaatcag ccgggctaca gctactggta
    12721 cgaatgcact tcgcgccatt ttactctggc tctcacgccg ctcagcgtgg cggataaatt
    12781 caaagagtta atggcgcaaa agcccggtag ctggatcttc acctcagcaa cgctgtcggt
    12841 gaacgacgat ctgcatcatt tcacctcgcg gcttgggata gaacaggcgg agtcgttgct
    12901 attgccaagc ccgtttgatt acagccgcca ggcgttactc tgtgtgccgc gcaatctgcc
    12961 gcaaaccaac cagccaggtt ctgctcgcca gttagcggca atgctgcgac cgatcatcga
    13021 agctaacaac ggtcgttgtt ttatgctttg tacctcgcac gccatgatgc gcgatctggc
    13081 cgagcagttc cgcgctacca tgacgcttcc cgttttgttg cagggggaaa ccagtaaagg
    13141 ccaactgttg cagcagtttg tcagtgccgg taatgcgctt cttgtggcaa ccagtagttt
    13201 ctgggaaggg gtggacgtgc gtggcgatac attgtcattg gtaattatcg acaaattgcc
    13261 gtttacctcg ccggatgatc cactgttaaa agcgcgcatg gaagattgtc gtttgcgcgg
    13321 tggcgacccg ttcgatgaag tgcaactacc agatgccgtc attactctca aacagggggt
    13381 aggtcgactg attcgcgacg ccgacgatcg tggcgtgctg gtgatttgtg actatcggct
    13441 ggtgatgcgt ccttacggcg cgacgtttct cgccagtctg ccgcccgcgc cacgcacccg
    13501 tgacattgcc cgtgcggttc gtttccttgc gataccatcc tccaggtaat ttgttacgga
    13561 gtatggtatg atgcgcgcct ttttattgat ctaagcacga agacccatgc gaattctggc
    13621 tatcgatacc gcgacagagg cctgctctgt cgccctgtgg aacgacggta ctgtcaacgc
    13681 tcattttgag ctttgccctc gtgaacatac tcaacgaatc ttaccgatgg tgcaggatat
    13741 cctgaccacc agcggaactt ccctgactga tattaacgct ctggcttacg ggcgcggccc
    13801 cggtagcttt accggcgtgc gcattggtat tggcatcgcg caagggctgg cgcttggcgc
    13861 ggaattaccg atgattggcg tctccacgct aatgacgatg gcgcaagggg cgtggcgcaa
    13921 aaacggcgca acacgcgtgc tgtcagccat tgatgcgcga atgggcgaag tttactgggc
    13981 cgaatatcag cgtgatgaaa acggtatctg gcacggtgaa gaaaccgaag ccgtactcaa
    14041 acccgaactc gttcatgaac gaatgcaaca gctttccggc gaatgggtga cggtagggac
    14101 gggctggcaa gcctggccgg atctcggtaa agagagcggg ctggttttgc gcgatggcga
    14161 agtgttactc cccgctgcag aagatatgct gccgattgct tgtcagatgt ttgccgaggg
    14221 taaaacggtg gcggtggaac atgccgagcc ggtttatttg cgtaacaacg tcgcatggaa
    14281 gaaacttccg ggcaaagaat gaatcttaag agtatgccct gagaaaaaaa ggagtcgcat
    14341 catggcggtt caaaagaatg ttatcaaagg catactggca ggtacgtttg cgctaatgct
    14401 gagcggttgt gtcactgtgc cggacgccat taaaggcagc agtaccacgc cgcaacaaga
    14461 tttagttcgg gtgatgagtg cgccgcagct gtacgttggt caggaggcac gctttggtgg
    14521 caaagtggtt gcggtacaaa accagcaagg gaaaacccgc ctggaaattg ctaccgtacc
    14581 gctggacagc ggagctaggc cgacgctggg agaaccttct cgcggtcgca tttatgccga
    14641 tgtgaacggt tttctggacc cggtggattt ccgtggacaa ctggttacgg tagtcgggcc
    14701 aatcaccggt gcggttgacg gcaaaatcgg caatacgccc tataaattta tggtgatgca
    14761 agtaacgggt tacaaacgtt ggcatttaac ccagcaggtg attatgccgc ctcagccgat
    14821 tgatccatgg ttttatggcg gtcgtggctg gccctatggc tacggcggat ggggctggta
    14881 taatcccggc cccgcgagag tacaaacagt tgtaaccgaa taattgcttg tttttaaaga
    14941 atgcagaaac agcggctggt ccgctgtttc tgcattctta cggtaaagat aaaaataaat
    15001 agtgacgcgc ttcgcaacct tttcgttggg taattatcaa gctggtatga tgagttaata
    15061 ttatgttaac ggcgtgtata tcatttgggg ttgcgatgac gacgaacacg cattttagag
    15121 gtgaagaatt gaagaaggtt tggcttaacc gttatcccgc ggacgttccg acggagatca
    15181 accctgaccg ttatcaatct ctggtagata tgtttgagca gtcggtcgcg cgctacgccg
    15241 atcagcctgc gtttgtgaat atgggggagg tgatgacctt ccgcaagctg gaagaacgca
    15301 gtcgcgcgtt tgccgcttat ttgcaacaag ggttggggct gaagaaaggc gatcgcgttg
    15361 cgttgatgat gcctaactta ttgcaatatc cggtggcgct atttggcatt ttgcgtgccg
    15421 ggataatcgt cgtaaacgtt aacccgttgt ataccccgcg tgagcttgag catcagctta
    15481 acgatagcgg cgcatcggcg attgttatcg tgtctaactt tgcccacact ctggaaaaag
    15541 tggttgataa aaccgccgtt cagcacgtaa ttctgacccg tatgggcgat cagctatcta
    15601 cggcaaaagg cacggtagtc aatttcgttg ttaaatacat caagcgcttg gtgccgaaat
    15661 accatctgcc agatgccatt tcatttcgta gcgcactgca taacggctac cggatgcagt
    15721 acgtcaaacc cgaactggtg ccggaagatt tagccttcct gcaatacacc ggcggcacca
    15781 caggtgtggc gaaaggcgcg atgctgactc accgcaatat gctggcgaac ctggaacagg
    15841 tcaacgccac ctatgggccg ttgctgcatc caggtaaaga actggtagta acggcactgc
    15901 cgctgtatca catttttgcc ctgaccatta actgcctgct gtttatcgaa ctgggtgggc
    15961 agaacctgct tatcactaac ccgcgtgata ttccagggtt ggtaaaagag ttagcgaaat
    16021 atccgtttac cgctatcact ggcgttaaca ccttgttcaa tgcgttgctg aacaataaag
    16081 agttccagca gctggatttc tccagtctgc atctttccgc aggcggtggg atgcctgttc
    16141 agcaagtggt ggcagaacgc tgggtgaaac tgaccggaca gtatctgctg gaaggttatg
    16201 gcctgaccga gtgcgcgccg ctggtgagcg ttaacccgta tgatattgat tatcatagcg
    16261 gtagcatcgg tttgccggtg ccatcgacgg aagccaaact ggtggatgac gacgacaacg
    16321 aagtaccacc gggtcaaccg ggtgagcttt gtgtcaaagg accgcaggtg atgctgggtt
    16381 actggcagcg tccggatgct acagatgaga tcatcaaaaa tggctggtta cacaccggcg
    16441 acatcgcggt gatggatgaa gaaggattcc tgcgcattgt cgatcgtaaa aaagacatga
    16501 ttttggtttc cggttttaac gtttatccca acgagattga agatgtcgtc atgcagcatc
    16561 ctggcgtaca ggaagtcgcg gctgttggcg taccttccgg ctccagtggt gaagcggtga
    16621 aaatcttcgt agtgaaaaaa gatccatcgc ttaccgaaga gtcactggtg actttttgcc
    16681 gccgtcagct cacgggatac aaagtaccga agctggtgga gtttcgtgat gagttaccga
    16741 aatctaacgt cggaaaaatt ttgcgacgag aattacgtga cgaagcgcgc ggcaaagtgg
    16801 acaataaagc ctgagcgtta agtcagtcgt cagacgccgg ttaatccggc gttttttttg
    16861 acgcccacta aagagaaaac aatttgaatt accaaatgat taccacggac gatgcgctgg
    16921 cttctttgtg tgaagccgtc cgtgtctttc cggcgatagc cctggatact gaatttgttc
    16981 gtacgcgcac ttattacccg cagctggggt tgattcaact tttcgatggc gagcatctgg
    17041 cgctaattga tcctctcggg atcaccgact ggtcaccgct gaaagcgatc ctgcgcgatc
    17101 cgtccatcac aaaatttctc catgcaggca gtgaagatct ggaagtgttc ctcaatgtct
    17161 ttggcgaatt accacaaccc ttgattgaca cgcaaatcct tgctgccttc tgcggacgcc
    17221 cgatgtcatg gggtttcgct tccatggtgg aagagtattc cggcgttacg ctggacaaga
    17281 gtgaatcgcg caccgactgg ctggccagac cgctgaccga acgtcagtgt gaatacgcag
    17341 cggcggatgt ctggtatctg ttaccgatca ccgccaagct tatggtagaa acggaggcct
    17401 ccggctggct acctgcggcg ctggatgaat gccgcctgat gcaaatgcgt cgtcaggaag
    17461 tcgttgcgcc ggaagatgcc tggcgtgata tcaccaatgc ctggcagtta cgcacacgcc
    17521 aactggcctg tctgcaactg ttagccgact ggcgactgcg caaggcgcga gagcgtgatc
    17581 tggcggtgaa ctttgtcgtg cgtgaagagc atttgtggtc ggtcgcgcgt tatatgccgg
    17641 gaagtttagg cgaactggac agcctgggtt tatccggtag cgaaatccgc tttcacggta
    17701 aaacgctgct ggcgctggtg gaaaaagcgc agacattgcc ggaagatgcc ttaccgcagc
    17761 cgatgcttaa cctgatggac atgcctggtt atcgtaaagc gtttaaagcg attaagtcgc
    17821 tgattactga cgtgagcgaa acgcataaga tcagcgccga attgctggca tcgcgtcggc
    17881 aaatcaacca actgctgaac tggcactgga aactgaaacc gcagaacaat ttgccggagc
    17941 tgatttccgg ctggcgtggt gagctgatgg cggaagcatt acacaattta ttgcaggaat
    18001 atccgcagta aaatcttccg aagccggact gggcgcgctc agcgccacat ccggctttgg
    18061 caaactacaa atccaacacc aggcgtttac ccttcgcacg cgaacaacag atcaacatac
    18121 tttgctggct ggcacgctct tcatcgctaa aatactgatc ccgatggtca gcttcacctt
    18181 ccagtattgc cgtttcgcag gttccgcata ccccttcacg acataaacat tccactttcg
    18241 cggctttatt attttcaata acctgcaaaa tagtcatctc ttccggcacc acaaactctt
    18301 ttccggaacg ggcaagcacc agggtaaatg catcgccggt tttgtcttcg atagcaaatt
    18361 gctcaaagtg cagtgtatcg gcggcgatgt ccagacgcgc agcttcactt cttaccgctt
    18421 caattagcgc ctcggggcca caggtataaa cgtgtgtgcc aggttcgata tccgccaata
    18481 atcgcgctaa ttccagtcgt gttccggttg atgaatgatg caaatggact ttctcagcct
    18541 gcggatgctg gactagctca gcacgaaatg cgcaactttc tggatttcgc gagcagtaat
    18601 gtagctgcca gtcgatgtcg ctgtgttgca actctgccat gtgcgacagg aaaggggtga
    18661 taccaatacc gcccacgata aacagatgct ttctggctga gggaattagc gcaaacaggt
    18721 tattaggcgt tgagatcgtt aaccgatcgc ccacttttac ctgctgatgc aaaaagcggg
    18781 aaccgccgcg cgagttttct tccagtcgaa cggcaatctg ataactagag gtgttatgcg
    18841 gtgaactcag tagtgaatac gcattgctgt actggttatc accatcgctc atctgcacta
    18901 ggatgtgact tcctccggtg aacgcaggca gcggtttgcc atcggttgcc accagcgtga
    18961 agcgtttcac ctgttcggta aggggttcaa cctggctcac ctgtacttca aacatttgat
    19021 agtctgacat tgctgtactc catcaaaaat cagcccagcg gcggaaaata ccgccgccga
    19081 tgtcattagt ccttaaacac ctgcgccagc agattatgga aatgggcgat accatgttcg
    19141 gaaatgccac taccgctact gtcggccatg atgcgcccct gaccacgata gccacgcgat
    19201 ttcagccctt tctgtacgct ttcaaccaga cgtaaatctt ccggatggaa cacatcgcga
    19261 taccactcaa tcagcgattt ttgctcgtcg gttaactctt cattggtgaa gtaaatatcg
    19321 tagttttgca gggtagtttc agaatccacc gggaattcat aaatgaccgt catcatccct
    19381 ttgatcgggg tgacgttcag catcgtgcac ggccacagcc agaaaccgtg gaatgccgca
    19441 tccgtaccct cttcaaattt aaacgactgt tcggacggtt tggcgaaacc gtattgcagc
    19501 gtccagttac cgtgcatggt gtgccagtaa cgatcaacct gtacggagtc ggagaaacct
    19561 agatgcgccg gaccacagtg atagcactcg agatagttat cgacaatgtt cttccagttg
    19621 gcaggcgtgc gggtggtaaa gcgggccgct agtttcagat tgtggacttc cgggcaggct
    19681 tccagcactt tcgcgcccag accggtgtat tgattcactt gaagtacgaa aaaaaaccgg
    19741 gaggacattg gattattcgg gttctgatgg gattagattt ggtggggctt gcaagcctgt
    19801 agcgcaaatt ttagtcgtta atcaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt
    19861 tcattgaaaa tacgaaaaac aaaaacactg caaatcattt caataacagc ttcaaaaaac
    19921 gttcaaaacc gataacaacc aggctgtaac caaatgactc atatcacaaa tcagctcatg
    19981 ccgtttaggt atgttacatg tgtgattatg tgaggtgaag tatgttttag ctggttcatg
    20041 gttgttatac ggcttttttt acatcttgtg gttcttgtga aggagctaca agaccttcct
    20101 gttcataaat accatacttt gacaaaatct ttttacgagt atctgttggt aatgattcaa
    20161 gcattttggc tgtttgtaag gtttgctgcg aaataccttg caaatcagcc tggatggaat
    20221 catcggaaga aaaaatctca ggcataagag cttcaaatcc tttctttctg aacaactcaa
    20281 tcgcagaatg cagttccttg tcctctaaca attcgacaat tgcgatcagt ttagttttca
    20341 atgtgctgcg gtcaggttga gaatttttat tctcttgatt atttttatgc tcaaccattg
    20401 gcatgctctg actttggata aaccactcta ttggacgccc tgttgcgtag gctatttgtg
    20461 ctaatcgatc taaagacggg tatgtcttgc cagacaggta gtttctgata accccatcag
    20521 aaagtccgca cttagctgca aacgcccggc ttgattctcc attcattgcc tcttcaaggc
    20581 gttccttgaa acgcattatt tccgggggca taaaaatgcg ctctgcattt ttgctttctt
    20641 tttcacctgt catgcccaat ctctctgtta tataaagaat tttacagaat tctttgattt
    20701 gcactggtta aaataaaaat gcgcatagct tttgaattga gcatttttct gcgctatcgt
    20761 ttttcccgat agcggtagcc ggtatcagtt accgaaaacg ttaagagagt aaggatcgca
    20821 gaatgtcagc aaaaaaccaa gactggcacg cagagcagat aaaggctgct gttcgtatga
    20881 aggggaagac actatctcaa
//
LOCUS       DAJTRW010000067        20709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRW010000067 DAJTRW010000000
VERSION     DAJTRW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO326_002684"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO326_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5967024.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO326_002685"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO326_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5967025.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO326_002686"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO326_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5967026.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO326_002687"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO326_002687"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5967027.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO326_002688"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO326_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5967028.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO326_002689"
     CDS             complement(8218..9111)
                     /locus_tag="OO326_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5967029.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO326_002690"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO326_002690"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5967030.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO326_002691"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO326_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5967031.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO326_002692"
     CDS             complement(10242..11474)
                     /locus_tag="OO326_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5967032.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO326_002693"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO326_002693"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5967033.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO326_002694"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO326_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5967034.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO326_002695"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO326_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO326_002696"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO326_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5967035.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO326_002697"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO326_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5967036.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO326_002698"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO326_002698"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5967037.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO326_002699"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO326_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5967038.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5029 g         4972 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatcctc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaat
//
LOCUS       DAJTRW010000068        20680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRW010000068 DAJTRW010000000
VERSION     DAJTRW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            80..349
                     /locus_tag="OO326_002700"
     CDS             80..349
                     /locus_tag="OO326_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5967039.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            343..708
                     /locus_tag="OO326_002701"
     CDS             343..708
                     /locus_tag="OO326_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5967040.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(709..1497)
                     /gene="bioH"
                     /locus_tag="OO326_002702"
     CDS             complement(709..1497)
                     /gene="bioH"
                     /locus_tag="OO326_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5967041.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1535..2218
                     /gene="gntX"
                     /locus_tag="OO326_002703"
     CDS             1535..2218
                     /gene="gntX"
                     /locus_tag="OO326_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5967042.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2277..2852
                     /gene="nfuA"
                     /locus_tag="OO326_002704"
     CDS             2277..2852
                     /gene="nfuA"
                     /locus_tag="OO326_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5967043.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3213..4529
                     /gene="gntT"
                     /locus_tag="OO326_002705"
     CDS             3213..4529
                     /gene="gntT"
                     /locus_tag="OO326_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5967044.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4574..6658)
                     /gene="malQ"
                     /locus_tag="OO326_002706"
     CDS             complement(4574..6658)
                     /gene="malQ"
                     /locus_tag="OO326_002706"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5967045.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6668..9061)
                     /gene="malP"
                     /locus_tag="OO326_002707"
     CDS             complement(6668..9061)
                     /gene="malP"
                     /locus_tag="OO326_002707"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5967046.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9673..12378
                     /gene="malT"
                     /locus_tag="OO326_002708"
                     /pseudo
     CDS             9673..12378
                     /gene="malT"
                     /locus_tag="OO326_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12421..13437)
                     /gene="rtcA"
                     /locus_tag="OO326_002709"
     CDS             complement(12421..13437)
                     /gene="rtcA"
                     /locus_tag="OO326_002709"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5967047.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13441..14667)
                     /gene="rtcB"
                     /locus_tag="OO326_002710"
     CDS             complement(13441..14667)
                     /gene="rtcB"
                     /locus_tag="OO326_002710"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5967048.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14848..16446
                     /gene="rtcR"
                     /locus_tag="OO326_002711"
     CDS             14848..16446
                     /gene="rtcR"
                     /locus_tag="OO326_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5967049.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16428..17186)
                     /locus_tag="OO326_002712"
     CDS             complement(16428..17186)
                     /locus_tag="OO326_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5967050.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17203..18033)
                     /gene="glpG"
                     /locus_tag="OO326_002713"
     CDS             complement(17203..18033)
                     /gene="glpG"
                     /locus_tag="OO326_002713"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5967051.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18078..18404)
                     /gene="glpE"
                     /locus_tag="OO326_002714"
     CDS             complement(18078..18404)
                     /gene="glpE"
                     /locus_tag="OO326_002714"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5967052.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18594..20099
                     /gene="glpD"
                     /locus_tag="OO326_002715"
     CDS             18594..20099
                     /gene="glpD"
                     /locus_tag="OO326_002715"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5967053.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20368..20598)
                     /locus_tag="OO326_002716"
                     /pseudo
     CDS             complement(20368..20598)
                     /locus_tag="OO326_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4888 a         5690 c         5213 g         4889 t
ORIGIN      
        1 tcagtagata ctctaccctg cttaagtgct actttgtgct atcttgcatg taacacaaag
       61 tagcacttag ggaactgata tgcagacagt gaaattacgg caacaaggag gagccatgat
      121 cgttactatc ccacgagatc ttgccatcga tcttggctgg tcaccgggca cagagcttac
      181 cgtggagaaa aaaggcgata gcgttaattt gcgcgccaca gagcacaaac cgcgtgggcg
      241 tcttaccgtc gcgcagttgt tgagccaaat cgatgaaagc gaaattacgg agctaaatca
      301 gtccacagaa ggatgggctg aagggaaaaa aggtaatgaa gcatggtaaa ggcacggacg
      361 ccacatcgtg gtgagatctg gtattttaac cctgatccgg ttgccgggca tgaacttcag
      421 gggccacatt attgcattgt ggtaacggac aaaaaactca acaatgtttt aaaagttgct
      481 atgtgctgcc cgatttcaac aggggcaaat gcagcacgtt ccacaggggt gacggtgaac
      541 gtcctccccc gtgatacgca aaccggtaac ctgcatggcg ttgtactttg tcaccagcta
      601 aaagccgtcg atcttattgc ccgtggcgct aaatttcata ccgttgccga tgaaaaattg
      661 attagtgaag ttatcagtaa actggtgaat ttaatcgacc cacaataatt agctctcact
      721 taccaccaac accctctgct tcaacgccac cagcaggtga caaaactcga ccggatgcga
      781 aataaacggc gcatgggccg ctttggcgaa gatatatgat tcgctgtgag gccaaagttt
      841 atccagcatc ggcaccactt tgcgcggcac cagaccgtcg agatagccat acaatcgcaa
      901 aaacggcatg gacacgtttt gcagcggctg acggagatcg accgttttca ggatttccag
      961 cccgccatta agcacgtcaa cctccggcat cggtaacgcc agaacggttt tcttcaacgc
     1021 ccgcgcatcc tggcgcgccg tttcagtccc catggtttgt aacgccagga accgctccac
     1081 tgtacgctga aaatcatcac tgagttgctg ctgaaatccc gccagcacgt ccggttttat
     1141 ccccggccac tcgtcacgag cactaaaaca aggtgacgac gccacggtga ccagcgcctg
     1201 aacacgctcg ggatgggtta acgcaatctg gcttgccacc agcccgccca gactccagcc
     1261 taaccaaatg gctttatcag gtgcctgttg cagcacggct tcggccatat cagcaagtga
     1321 cagcgcacca aatccccggc tacgcccgaa gccgggcagg tcaacaaggt gcagcgtaaa
     1381 atgcgagcta agttcctcgt caatgcaacg ccacacttcg gcattcagtc cccatccgtg
     1441 cagcagcaca agatgaacat tcccctgacc tttggtctgc caccagatgt tattcatccg
     1501 ctattgttct cttttgactt acaaggatga acatatgcta acagtaccgg gattatgctg
     1561 gctatgccga atgccactgg cgttaggtca ttgggggatt tgttcggtct gttcacgcgc
     1621 cacccgcaca gataaaacgt tatgcccaca atgtggatta cccgccacac actcccatct
     1681 tccctgcggt cgctgcctgc aaaaaccgcc gccctggcaa agactggtca cggttgccga
     1741 ctatgcgccg ccgttaagtc cgcttatcca ccagcttaaa ttttcccggc gcagtgaaat
     1801 cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta cacgctcgtc gcaccaccgg
     1861 gttgcaattg ccggatcgca tcatcagcgt tccgttatgg cagcggcgtc actggcgtcg
     1921 gggatttaat cagagcgatt tgctgtgtca gccgttatca cgctggttgc actgccaatg
     1981 ggatagcgaa gccgtcacac gtacacgggc cactgcgacc cagcattttc tcagtgcccg
     2041 gctgcgcaag cgcaacctga aaaatgcctt tcgtcttgaa ttgcccgtgc aaggtcgcca
     2101 tatggtgatt gtggatgatg tcgttaccac cggaagtacc gtcgcagaga ttgcgcagtt
     2161 gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc ctttgtcgaa ccttgtagag
     2221 cctcgatgat gggcgtatta taaccaacta aaatagtcaa ctattaggcc attactatga
     2281 tccgtatttc cgatgctgca caagcgcact ttgccaaact gctggcaaat caggaagaag
     2341 ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc taacgctgaa tgtggtgttt
     2401 cttattgtcc gccggatgcc gtggaagcca ccgacactgc cctgaaattt gacctgctga
     2461 ccgcgtatgt tgatgagtta agcgcaccat acctggaaga tgcagagatc gactttgtta
     2521 ccgaccagtt gggttctcag ttaacgctga aagccccgaa cgccaaaatg cgtaaagtgg
     2581 cagacgatgc accgctgatg gagcgcgtgg agtatatgct gcagtcgcag atcaacccac
     2641 agcttgctgg tcacggtggt cgcgtttcgc tgatggaaat caccgaagac ggttacgcca
     2701 ttctgcaatt tggcggcggc tgtaacggtt gttccatggt cgatgtgacg ctgaaagaag
     2761 ggatcgagaa gcagctgctg aacgaattcc cggagctgaa aggtgtgcgc gatctcaccg
     2821 aacaccagcg cggcgaacac tcctactact aagttatccc ctcttttgta gattgcccga
     2881 tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg tgccagacgt tggcacagca
     2941 ttgctttggc aaatccctcc cttcgtttcc ccgcgataat atgaccaacc tctcataatt
     3001 taaatttacc ccgctctggt gattctcaaa cgccaaatgt tacccgtatc attcacatgg
     3061 gtaccaaaca tactcctgac atctgactac aataattagt tttagtgggt atcagtcgtg
     3121 gtgccgcaat atctctgttc ccgattggga taattagagt ttgtcgtcag aaaattgacg
     3181 ttacccataa caaatgaaag gccaggtaaa tcatgccatt agtcattgtt gctatcggtg
     3241 taatcttgtt gttgctcctg atgatccgct tcaaaatgaa cggcttcatc gctctcgtcc
     3301 tcgtggcgct tgctgttgga ttaatgcaag gaatgccgct ggataaagtt attggctcca
     3361 tcaaagccgg tgtcggcggg acgctcggta gccttgccct gatcatgggt tttggcgcaa
     3421 tgctgggcaa aatgctggca gactgcggtg gcgcacaacg tatcgccacc acgctgattg
     3481 ccaaatttgg taaaaagcac atccagtggg cggtggtgtt aaccggtttt accgttggtt
     3541 ttgccctgtt ctatgaagtg ggcttcgtgc tgatgctgcc gctggtgttt accatcgcgg
     3601 cttccgcgaa tattccattg ctgtatgttg gtgtaccaat ggcggctgca ctgtctgtga
     3661 cccacggctt cctgccaccg catccgggcc cgactgcgat tgccaccatt ttcaatgccg
     3721 atatgggtaa aaccctgctg tacggtacta ttctggcaat cccgaccgtg attctcgccg
     3781 gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc aatcccggaa ggtctctaca
     3841 gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg cgtcagcgtc tggacctctc
     3901 tggtgccggt tgtgctgatg gcgatgcgtg cgattgccga aatgatcctg ccgaaaggtc
     3961 acgctttcct gccggtagcg gagttcctcg gtgacccggt aatggcaacg ctgattgccg
     4021 tgctgattgc gatgttcacc tttggtctga accgtggtcg ttcaatggat cagattaacg
     4081 acacgctggt ttcttccatc aaaatcattg cgatgatgct gttgatcatc ggtggtggcg
     4141 gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa atacattgct tccatgatgc
     4201 acgaaaccaa catttctccg ctgctgatgg cctggtcgat tgctgccgta ctgcgtatcg
     4261 cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg tatcgcggca ccgctgattg
     4321 caacgacggg tgttagccca gagctgatgg ttattgcggt gggttccggt agtgtgattt
     4381 tctctcatgt gaacgatccg ggcttctggc tgttcaaaga gtactttaac ctgactatcg
     4441 gcgagaccat caaatcctgg tcgatgctgg aaaccattat ctcggtgtgc ggtctggtag
     4501 gctgtctgct gctgaatatg gtgatttgat aacactgact gccggatgca tcgcatccga
     4561 catcaaccgc actctacttc ttcttcgctg cagctctgcg ccgtctgtcc aaatccttca
     4621 gcaacttgtt cacgccatca tcggcaaaca tcgactcaag cgttgcggaa agcttgcgtc
     4681 gccagttttt atactggtaa ctggtgccag gaatattcac cggttcggcc atatccagcc
     4741 agtcttccgg ctgtagtcct aacagagcac tgttactgtc ggcaatgtag cgctgcaaac
     4801 cacggttcag cgtcggcgtc atcgacatca acgatgcctt atgcccggca cgttttggca
     4861 gacaaccata tttatgcagt gcatccagca gcccttgctt cgccagttcg cgatcctgat
     4921 acagaccgcg cagtaccact tcatccggat acagccccag ggttttgccc agcgttagat
     4981 ccccgctctc ccagtaacca cgcagcgttg gcaggtcatg tgtcgccgca accgccatcg
     5041 actgctccgg atacgctttc ggtgcacgga acgtcttctc gtggtcgttt tcgaaataga
     5101 gcactttgta agagtacaca ccgctgctgc gcagcttacc aacaatctct accggtacgg
     5161 taccgagatc ttcaccaatc accatacagc gatgacgttt actttcgagt gccagaatcg
     5221 agagcagatc atccaccgga tagtgaacat acgcgccctg atctgccgtc tcgccatacg
     5281 gtatccacca caaacgcagc atcgacatca catggtcaat tcgtaatgcg ccgcagtttt
     5341 gcatattggc acgcaacagc tcgataaacg gttcataggc acgcgcggtg atgatatgcg
     5401 ggtccattgg cggtaatccc cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc
     5461 caaccgatgc tttcaggcaa tatagttcac ggtcacacca ggtttccgcc ccaccttccg
     5521 ctacgccaac cgccagatca cgatacaagc caatcggcat ttcatagccc tggcttatct
     5581 cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa ccagagataa aaatcgacgt
     5641 catcacgatg ttcttcgcag aactgacgca cttctggtga atccacgttc ttatacatct
     5701 ctggccatgc aggccagccc cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg
     5761 catcaaaggc agcctgccag aacaggctgt cgccctgctc tgcaacaaac tggcgaaacg
     5821 cggccatctg ctcatcatca cgttgcgcga aacctttcca cgccattcgt aatgctgtca
     5881 tttttagggc ggtaaccgtg gagtaatcga cccagtcggc atcgcgcgcc tgttgcagcg
     5941 tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc ttcgctaaga tggaaatctt
     6001 caacggcgtt aacgtcgata taaatcacat tcagccaacg gcgagaagac gggctgtatg
     6061 ggctggcgct ctccggattt gccggataga gcgcatgaat cgggttcagg ccaatgaacg
     6121 acccgccacg ttttgccaca tccaccagca tcgctttgag atcgccaaaa tccccaatac
     6181 cccagttttt ttccgatcgc agcgtataaa gctgaacgca ggcaccccac agcttttgtt
     6241 tattcagcaa agcctgcggt tcgtaacaac gtttcggggc gacaatcacc cggcaatgcg
     6301 cgcgctggtc gtcctgggtg agtgttagcg tgtgataacc ttccggcagc ttcgtcggta
     6361 gattgaacgc tttgcccccc gttacatggc ctttgtactg cgttccttct tcggtggtca
     6421 gctgccagct atattcgccg ctgccctcca ccaccatcgg catttttttg ccgctggtat
     6481 aaaccatgac attcgggact ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg
     6541 cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg cggtttaccg tgggcattga
     6601 tgtaattggg gctaatcccc gccgccagcg cggcattatc cagacgtttg ctttccatcg
     6661 agcttcctta gcgttttgcc tgccagatac gagcctgata atcgcgaata gagcgatccg
     6721 agctaaacat accgcagcgg gcggtattga ggatcgccgc gcgagtccag gcctcctggt
     6781 cgcggtacag cacatccacc tgcttttgtg cctcaacata ggctgcgaaa tccgccatca
     6841 ccagatacgg atcgccgccc tgtttgccga tactgtgcag catctggtcg aaggcatgct
     6901 tatcgccgtc gctgtattta ccgctttcca gctctttcaa tactgcgtcc agcaccttat
     6961 ctttcttccg ccatttcacc gggtcgtagc ctttggccag aatagccttc acttgttcca
     7021 cggtatgacc aaaaataaag atattttctt caccgacttt ctcggcgatt tcaacgttcg
     7081 caccatccag cgtaccgaca gtaagcgcac cattgagcgc cagtttcata ttgccggtac
     7141 cggaagcttc tttacctgca gtcgaaattt gttcggagat atccgccgcc gggatcagtt
     7201 tttccgccgc cgaaacgcaa taatccggca ggaacaccac cttcaactta tcgccaacca
     7261 gtggatcgtt gttgatcacg tcagccactt tgttgatcgc aaagataata ttcttcgcca
     7321 ggtagtagcc cggtgccgct ttcgcgccga agaggaagac gcgcggtacg cgatcagcct
     7381 gcaggttttc acgaatttct ttgtacaacg ccagaatatg cagcagattc aggtgctggc
     7441 gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa
     7501 taccggtacg aactttcaca aactccgcca gacagacttt attcgcctgc ttgatctcgc
     7561 gatattgctg acggaatttc gcatcatcag cgaatttttc cagattgatc agctgatcga
     7621 gatcgttagc ccactctttt tgcagtgatt tatccaacag agccgccagt gcagggttgc
     7681 actgtttgat ccagcgacgt ggggtaatac cgttggtaac gttatggaat ttgttcggcc
     7741 atagctggtg atattccggg aacagatctt tcaccaccag atccgagtgc agcgccgcaa
     7801 caccgttcac cgcgaaaccg ccaaccacac acaggttcgc catatgcact tgtttatcgt
     7861 gcaccaccgc cagtttggcc cacacttttt catcgcccgg ccaggttttc tccaccagcg
     7921 ttttaaagcg agtattaatt tcgttaataa tctgcatatg gcgcggcagt aagcctttca
     7981 ccagtttcac atcccagcgt tccagcgctt ctggcatcag ggtatggttg gtgtaagcga
     8041 aagttttgct ggtgatggcc caggcgtcat cccagctcat ctggtgctca tcggtcagca
     8101 cgcgcagcag ttctggaatc gcgatagttg gatgggtatc gttcagctga ataacttcgt
     8161 aatccgccag ttcgtgcagt ttacgccccg ccagatgatg gcgacgcaaa atatccgcta
     8221 ccgaacaggc acactggaag tattgctgca tcaggcgcag ctttttaccg gcagtatggt
     8281 tgtcgtttgg atagagaact ttggtcagtt tttccgcatt gatgccttgc tgttcggcgc
     8341 gcaggaaatc accgtcgtta aatttagtta gatcaaacgg atgcgcatgc gtcgcctgcc
     8401 acagacgcag cggctgcgcc acgccattac gatagccgac aacggggaga tcccacgctt
     8461 gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt catcacttta ccgccaatcc
     8521 ctacctgcac atccagtgct tcgttgtggc ggaaccacgg gtagttactg cgatgccagt
     8581 catccggcgc ttcaacctgt ttgccatcga caaaagactg gcggaacaaa ccatattgat
     8641 agttcagacc gtaacccgtc gcagactgac cgacagttgc cattgagtcg aggaagcacg
     8701 ccgccagacg tcccagacca ccgttaccca gcgccgggtc gatctcttct tccagcaggt
     8761 cagtcaggtt gatgtcataa gccttcaacg aatcctgtac atcctgatac cagccgagat
     8821 tcaacaggtt gttgcccgtc agacgaccaa tcaaaaactc cattgagatg tagttaacat
     8881 gtcgctgatt cgccaccggt ttggcgaatg gctgagcacg cagcatttcg gccagtgctt
     8941 cactcactgc cagccaccac tggcgaggag tcatttcagc cgcagaattt aagccataac
     9001 gctgccactg acgtgaaagc acttcctgaa attgcttatc gttaaaaata ggttgtgaca
     9061 taggagttcc acttttctta gattttcaac acaacgttat cgctagtttg ccaggctcga
     9121 tgttgacctt cctcatcctg cgggggatta ggcagggagg agttgcgggg atgagcaagg
     9181 aaatgtgatc tcgaccactt aaagctagtg caaaccacag gattagcagc aaatcaatgc
     9241 catacagcgc agaaaatctg tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa
     9301 gcggaaggta actctataaa ttaagtaaag gagtgaaaca gtttcacaag taaaatatcc
     9361 agtgtgctcc atctcattct taatagattt attaagatca tctttttaga tggcactttc
     9421 atcaggaatg aagaagaaac ccttgcttaa atgaatctga tgaacataag ggaaaccagt
     9481 attcacgctg gatcagcgtc gttttaggtg agttgttaat aaagatttgg aattgtgaca
     9541 cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc attagaaagg tttctggccg
     9601 accttataac cattaattac gaagcgcaaa aaaaataata tttcctcatt ttccacagtg
     9661 aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc cggttcgact cgaccatacc
     9721 gtggttcgtg agcgcctgct ggctaaactt tccggcgcga acaacttccg gctggcgctg
     9781 atcacgagtc ctgcgggcta cggaaagacc accctcattt cccagtgggc ggcaggcaaa
     9841 aacgatatcg gctggtactc gctggatgaa ggtgataacc agcaagagcg tttcgccagc
     9901 tatctcattg ccgccgtgca gcaggcaacc aacggtcact gtgcgatatg tgagacgatg
     9961 gagcaaaaac ggcaatatgc cagcctgacg tcactcttcg cccagctttt cattgagctg
    10021 gcggaatggc atagcccact ttatctggtc atcgatgact atcatctgat cactaatcca
    10081 gtgatccacg agtcaatgcg cttctttatt cgccatcaac cagaaaatct caccctggtg
    10141 gtgttgtcac gcaaccttcc gcaactgggc attgccaatc tgcgtgttcg tgaccaactg
    10201 ctggaaattg gcagtcagca actggcattt acccatcagg aagcgaagca gttttttgat
    10261 tgccgtctgt catcgccgat tgaagccgca gaaagcagtc ggatttgcga tgacgtttcc
    10321 ggttgggcga cggcactata gctaatcgcc ctctccgccc ggcagaatac ccactcagcc
    10381 cataagtcgg cacgccgcct ggcgggaatc aatgccagcc atctttcgga ttatctggtc
    10441 gatgaggttt tggataacgt cgatctcgca acgcgccatt ttctgttgaa aagcgccatt
    10501 ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg gcgaagaaaa cgggcaaatg
    10561 cgtctcgaag agattgagcg tcaggggctg tttttacagc ggatggatga taccggcgag
    10621 tggttctgct atcacccgct gtttggcaac ttcctgcgcc agcgctgcca gtgggaactg
    10681 gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa gctggatggc ccagggattt
    10741 cccagcgaag cgattcatca tgcgctggcg gcaggcgatg cgctgatgct gcgcgatatt
    10801 ctgcttaatc acgcctggag tttgttcaac catagcgaac tgtcgctgct ggaagagtcg
    10861 cttaaggccc tgccgtggga cagtttgctg gaaaatccgc agttagtgtt attgcaggcg
    10921 tggctaatgc aaagccaaca tcgctatggc gaagttaaca ccctgctagc ccgtgctgaa
    10981 catgaaatca aggacatcag agaaggcacc atgcatgcag aatttaacgc tctgcgcgcc
    11041 caggtggcga ttaacgatgg taatcaggat gaagcggaac ggctggcaaa actggcactg
    11101 gaagagctgc cgccgggctg gttctatagc cgcattgtgg caacctcggt gctgggtgaa
    11161 gtgctgcact gcaaaggcga attgacccgc tcactggcgc tagtgcagca aaccgaacag
    11221 atggcacgcc agcacgacgt ctggcactac gccttatgga gtttaattca gcaaagtgaa
    11281 attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa cgcaggaaaa agcgttccag
    11341 ctgatcaacg agcagcatct ggaacagctg ccaatgcatg agtttctggt gcgcatccga
    11401 gcacaactgt tatgggcctg ggcacggctg gatgaagccg aagcgtcggc gcgtagcggg
    11461 attgaagtct tgtcgtctta tcagccacag caacagcttc agtgcctggc aatgttgatt
    11521 caatgctcgc tggcccgtgg tgatttagat aacgcccgta gccagttgaa ccgtctggaa
    11581 aacctgctgg ggaatggcaa atatcacagc gactggatct ctaacgccaa caaagtccgg
    11641 gtgatttact ggcaaatgac cggcgataaa gccgccgctg ccaactggtt gcgtcatacg
    11701 gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc aatggcgcaa cattgcccgc
    11761 gcacaaatct tgctgggcga gtttgaacct gcggaaattg ttctcgaaga actcaatgaa
    11821 aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta acctgttgct gcttaatcaa
    11881 ctgtactggc aggccggacg taaaagtgac gcccagcgcg tgttgctgga cgcattaaaa
    11941 ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg aaggcgaagc gatggcgcaa
    12001 caactgcgcc agctgattca acttaatacg ctgccggaac tggaacagca tcgcgcgcag
    12061 cgtattctgc gagaaatcaa tcaacatcat cggcataaat tcgcccattt cgatgagaat
    12121 ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac taatccgcac cagcccgttg
    12181 acccaacgtg aatggcaggt cctggggctg atctactccg gttacagcaa tgaacaaatt
    12241 gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc atatccgcaa tctgtaccaa
    12301 aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg cgcagaaact gctgaagatg
    12361 atgggttatg gggtgtgagt ttagccggat aatgcattgg atccggttta catctttaca
    12421 tcattcaata ctcacccgcg ttacgccatc tgtttctatc aaaccaaacc gcaccggcaa
    12481 gaaacgctcc accaccgcga tattggtcag cagatggcat gaaggatggg cgaccgtaaa
    12541 ttctcccgcg cccgccagcg ccatcggcag taccagttgg tcggcgagat attcccccac
    12601 cgccgctggg cttgccaggt agcgtttcac ctctttcact aactgcgcgg ctaccacctc
    12661 ggcactgacg cgcttttcac cgacgacgaa aaagcgttca gtgatatttt cactttcgac
    12721 ttccagcgag acggtattac ccggcccctg gtcgcgcggc aggttatgaa tattctgttc
    12781 atgcagggaa aaaatccccg ccagtgtagc gatttcacgc tcagcaacat ggcgcggcac
    12841 accagctaat agaacttctc cacgcatctg cacaatgttc ccgcgctcgc caagttgcaa
    12901 ggtgctaaac gatgccacgg gcgagacttc cgttgccacc acaccgcctc cggcaggata
    12961 aaaaccgtga cgtaacagcg tggtttgctg atgaattcct attttcgcca gcagcggctc
    13021 cagcacccgg cggataaaat ccgcaggcgg cgccgacggg ttatcggtgc cgccgctcac
    13081 ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc agcaccgttt gcagcaccag
    13141 cgtacaactt ccggcgctac cgatagcaaa gcggtaatcg ccgccgcgca cggtgccagg
    13201 ccggaagacc agacgctgcg atcccagctc cgcgccttcc accgttgccc tacaaatttc
    13261 cgcagccgct tttaccgcgg tcagatgctg gcgcagcagc cccggtttcg cccgtccggc
    13321 tcgaatgcca gtgatggtaa atggctggcc ggttatcatc gacaggctca gcgccgagcg
    13381 caggatctgc ccgccgcctt cgccctgtgc gccatccagc gcaatcatcc ttttcatcat
    13441 ttatcctttt acgcacacca cctgacgcag ggtgtagata acttccacca gatcgctttg
    13501 tgccgccatc accgcatcaa tatctttata cgccatcggg atttcgtcga tcacttcggc
    13561 atctttacgg cattccacat gcgcggtggc acgaatttgg tcttccacgc tgaacagttt
    13621 tttcgcttta gttcggctca ttacccgccc ggcaccgtgg ctgcacgaac agaacgactc
    13681 ttcatttccc agcccacgga cgataaagct ttttgctccc atcgaaccgg gaataattcc
    13741 atattgacca gcacgcgcag acaccgcacc tttacgcgtc acgtagatct cttcaccaaa
    13801 gagctgttct ttttgcacat agttgtggtg acagttgatc tcttccatcg ccagcgtttg
    13861 tggctgtctg accgttttct gcgtaatgct ctgcaacgcc gttaccacat tttccatcat
    13921 cgcatcgcgg ttaaggctgg cgaaaagctg cgcccaggcc acggctctta ggtaatcatc
    13981 aaagtattcc gtaccttcca taaagtacgc cagatcacgc gacggcaacg tctcaagttg
    14041 gtcctgcatc tctttttgcg ccagatcgat aaagtaagtc ccgatggcgt taccaattcc
    14101 gcgtgaaccg gagtgcagca taatccacac ctgctccgac tcatcaaggc agatttcaat
    14161 aaagtggtta cccgttccca gcgttcccag gtgtttatag ttgttagtgt taaggaaacg
    14221 cggatatttt tgcgttaacc actgataacc ggcctccagc tcagcccatt tcgcatcgac
    14281 gttaaaaggc gggttttccc atgcgccttt atcacgttta caacgcccgg tagtacgccc
    14341 gtgtggtacg gccgtttcaa tcgcttgacg cagctccgcc aggttttcag gcaggtctgc
    14401 cgccgttaac gcggtacgca gcgcgttcat tccacagcca atatccacgc ccaccgccgc
    14461 cggaataatc gcccctttgg tcgggatcac gctaccaatg gtggaacctt tacccaggtg
    14521 tacatcaggc attaccgcaa tatgtttgaa aataaacggc atcttcgccg tattaataag
    14581 ttgctgacgc gcatcggctt ctaccggcac gcctttggtc cacattttta ccggggcatt
    14641 ttcagtggtc agtaattcgt aattcatttt gtttttctct tttcgttgtt tgcggtcctg
    14701 ataattgcaa ccgtcgtgcc agaaaattaa ataagcagat taatttattg tttctaaaaa
    14761 gattatatct ttatatccgt aaacggagat ttcccgcaaa gccgatttac cgataatgaa
    14821 atatcgcctt ttataaggat atctaagatg cgtaaaacag tggcttttgg ctttgtcggt
    14881 accgtgctgg attatgccgg acgcggcagt cagcgctggt caaaatggcg tccgtcactc
    14941 tgtatatacc agcaagaatc gttggtcatc gatcgactgg aattattgca cgatgcccgc
    15001 tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa
    15061 gtggtgggcg ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc
    15121 ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta tttaattcac
    15181 atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggcgga agcgcgttat
    15241 ctacccgccc ggttgataca atcttcaact ccgcgcaaaa aagaacagcc gcgcggccca
    15301 ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc cagccgcttt
    15361 gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg gtattgccac gcgtaacccc
    15421 cacttcaacc gcatgattga gcagatcgaa aaagtggcga ttaaatcccg cgcgccgatt
    15481 ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg cgcgacgcat cttcgagtta
    15541 aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc
    15601 ggcgataccg cgatgtcgac gctgtttggt catgtgaaag gcgcgtttac cggggcgcgg
    15661 gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct tgatgagatt
    15721 ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ctattgaaga gaaaaccttt
    15781 tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg
    15841 cgcgatttgc gccagctggt tgccgaaggc aaattccgcg aagatctgta cgcgcggatc
    15901 aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat tgaaccgaac
    15961 ctggattatg aagtggagcg ccacgccaca ctcactggtg acagcgtgcg ttttaacacc
    16021 gaagcgcggc gtgcctggct ggcctttgca acatctcccc aggcaacatg gcgcggtaac
    16081 tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag cggacgcatc
    16141 actctggacg tggtggaaga tgagataaac cgtctgcgct ataactggca ggagagccgc
    16201 ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt
    16261 atgcaactgg aacacgttat cgctatctgc cgccaggcaa agtcgctttc tgctgccgga
    16321 cgacagcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta
    16381 cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc
    16441 agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg ggcgtcggtg
    16501 tagacggcat caaccatgct gatgctcccc atattgacca tcgcattacg gccaaatttt
    16561 gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa taatggcgcg cttggtacga
    16621 acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt cgatgccgct gatccccaga
    16681 atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg
    16741 ccatcgcggc tacgtaattc gccaccggcg agaataatgc ggaaatcttc ttttaccatc
    16801 aacgtgttag caacgttgag attgttggtg acaatgcgca aattgctgtg attgagcagc
    16861 gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga gccattgggg
    16921 atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt ggctttacga
    16981 tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg cgccgccatg atggcgcagg
    17041 atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa tagtctgcgg gctgacggag
    17101 aagtgctcca ccagttcctc ggtactgaca taaccctgct gtttaaccag ttcgataata
    17161 ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc gttttcgcgc
    17221 attgagcgaa tcaacaaaag ccatcgctaa acccacggct aatccggcga tgtgtgctcc
    17281 gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa
    17341 gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc cacgtagcca
    17401 gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa
    17461 tttttgctgc atatagccgc ttaacagggc gctgataagc gtaatgacaa ttagcttacc
    17521 gctaccgagg cgtttttcca ccgcgccgcc gagataccac caccaaagca ggttaaagag
    17581 gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt
    17641 cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc cgagaatttg
    17701 catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg gacctgcgcg
    17761 ttcacgcaag gcagcaaaga aaggataacg gcgataatgc aggccactgc cggtatggcc
    17821 tgccagccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag
    17881 ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg
    17941 ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac
    18001 gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa
    18061 ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg tcgctgccag
    18121 gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc
    18181 gccgcccctt tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg
    18241 ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc
    18301 cccatagcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac
    18361 ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct ctctttcttt
    18421 acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa
    18481 tgttatacat atcactctaa aatgtttttt caatgttacc caaagcgcga ttctttgcta
    18541 atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc agcatggaaa
    18601 ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg
    18661 ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt
    18721 ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc
    18781 tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg catatcgcct
    18841 tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgca
    18901 ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga tcaactggtt
    18961 tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc gaatattctg
    19021 actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag
    19081 gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga
    19141 ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg
    19201 ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc
    19261 cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg catacccaaa
    19321 agcaggccta cattctgcaa aacgaagata aacgcattgt gttcgtgatc ccgtggatgg
    19381 acgagttttc catcatcggc accaccgatg tggaatacaa aggtgatccg aaagcagtga
    19441 agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac tttaaaaagc
    19501 agctaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg
    19561 agtccgactc gccgcaagcc attacccgtg attacaccct tgatatccat gatgaaaacg
    19621 gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcag
    19681 aacatgcgct ggaaaaactg acgccgtatt atcagggtat tggcccggcg tggacgaaag
    19741 agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattacgcc gctcgcctgc
    19801 gccgccgtta cccgttcctg actgaatcgc tggcacgtca ctacacgcgc acttacggca
    19861 gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg gaagatttcg
    19921 gtcatgagtt ctacgaagcg gaactgaaat acctggtgga tcacgaatgg gtccgccgca
    19981 ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat
    20041 ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaaa
    20101 ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac aaacacaaaa
    20161 ctgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatatt gaatttgcat
    20221 ggtcttgtag gccagataag acgttcacgt cgcatccggc atgaacaaag cgcactttgt
    20281 caaaaatcta acctactttt taattcagga aattaccgca aagcccacgc catcataccc
    20341 aacgtaacaa aactcaggca cgttccccac tcgccccgag aaaaatagca ttaatgcgcc
    20401 cagcgtcagc ataaaaatat tgagcagaag gtaaattgtt ggcgtaaaga gtaaattgca
    20461 aacctcagga atagcggcaa gcccagcccc cagcatggca ataaccgctg caaaaaattg
    20521 cgtcagtatt gggatgccga aaaaagtcat taccagcgag gtcaaaatcc attttctatt
    20581 ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag tccccttgcc
    20641 ccttctggac acttttccga aatgatggcg aaaaaaacgg
//
LOCUS       DAJTRW010000069        20405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRW010000069 DAJTRW010000000
VERSION     DAJTRW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            127..468
                     /gene="raiA"
                     /locus_tag="OO326_002717"
     CDS             127..468
                     /gene="raiA"
                     /locus_tag="OO326_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5967054.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    571..696
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            572..619
                     /gene="pheL"
                     /locus_tag="OO326_002718"
     CDS             572..619
                     /gene="pheL"
                     /locus_tag="OO326_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5967055.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            718..1878
                     /gene="pheA"
                     /locus_tag="OO326_002719"
     CDS             718..1878
                     /gene="pheA"
                     /locus_tag="OO326_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5967056.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1921..3042)
                     /gene="tyrA"
                     /locus_tag="OO326_002720"
     CDS             complement(1921..3042)
                     /gene="tyrA"
                     /locus_tag="OO326_002720"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5967057.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3053..4123)
                     /gene="aroF"
                     /locus_tag="OO326_002721"
     CDS             complement(3053..4123)
                     /gene="aroF"
                     /locus_tag="OO326_002721"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5967058.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4333..4698
                     /locus_tag="OO326_002722"
     CDS             4333..4698
                     /locus_tag="OO326_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5967059.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4848..5366
                     /locus_tag="OO326_002723"
     CDS             4848..5366
                     /locus_tag="OO326_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5967060.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5356..6581
                     /gene="dgcN"
                     /locus_tag="OO326_002724"
                     /pseudo
     CDS             5356..6581
                     /gene="dgcN"
                     /locus_tag="OO326_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6597..7079
                     /locus_tag="OO326_002725"
     CDS             6597..7079
                     /locus_tag="OO326_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5967061.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7156..7503)
                     /gene="rplS"
                     /locus_tag="OO326_002726"
     CDS             complement(7156..7503)
                     /gene="rplS"
                     /locus_tag="OO326_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5967062.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7545..8312)
                     /gene="trmD"
                     /locus_tag="OO326_002727"
     CDS             complement(7545..8312)
                     /gene="trmD"
                     /locus_tag="OO326_002727"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5967063.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8343..8891)
                     /gene="rimM"
                     /locus_tag="OO326_002728"
     CDS             complement(8343..8891)
                     /gene="rimM"
                     /locus_tag="OO326_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5967064.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8910..9158)
                     /gene="rpsP"
                     /locus_tag="OO326_002729"
     CDS             complement(8910..9158)
                     /gene="rpsP"
                     /locus_tag="OO326_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5967065.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9295..10656)
                     /gene="ffh"
                     /locus_tag="OO326_002730"
     CDS             complement(9295..10656)
                     /gene="ffh"
                     /locus_tag="OO326_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5967066.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10823..11614
                     /locus_tag="OO326_002731"
     CDS             10823..11614
                     /locus_tag="OO326_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5967067.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11635..12921
                     /locus_tag="OO326_002732"
     CDS             11635..12921
                     /locus_tag="OO326_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5967068.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12976..13569)
                     /gene="grpE"
                     /locus_tag="OO326_002733"
     CDS             complement(12976..13569)
                     /gene="grpE"
                     /locus_tag="OO326_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5967069.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13692..14570
                     /gene="nadK"
                     /locus_tag="OO326_002734"
     CDS             13692..14570
                     /gene="nadK"
                     /locus_tag="OO326_002734"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5967070.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14656..16317
                     /gene="recN"
                     /locus_tag="OO326_002735"
     CDS             14656..16317
                     /gene="recN"
                     /locus_tag="OO326_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5967071.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16466..16807
                     /gene="bamE"
                     /locus_tag="OO326_002736"
     CDS             16466..16807
                     /gene="bamE"
                     /locus_tag="OO326_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5967072.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16869..17159)
                     /locus_tag="OO326_002737"
     CDS             complement(16869..17159)
                     /locus_tag="OO326_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5967073.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17149..17586)
                     /gene="ratA"
                     /locus_tag="OO326_002738"
     CDS             complement(17149..17586)
                     /gene="ratA"
                     /locus_tag="OO326_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5967074.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17757..18239
                     /gene="smpB"
                     /locus_tag="OO326_002739"
     CDS             17757..18239
                     /gene="smpB"
                     /locus_tag="OO326_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5967075.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18454..18816
                     /gene="ssrA"
                     /locus_tag="OO326_002740"
     tmRNA           18454..18816
                     /gene="ssrA"
                     /locus_tag="OO326_002740"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19021..20262
                     /locus_tag="OO326_002741"
     CDS             19021..20262
                     /locus_tag="OO326_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5967076.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5268 a         5333 c         4797 g         5007 t
ORIGIN      
        1 ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga caaaattatg agatttccat
       61 cacacatttt gacatcagga acggtatgct gaattcacca agacgggaag acaagaggta
      121 aaatttatga caatgaacat taccagcaaa caaatggaaa ttactccggc catccgccaa
      181 catgtcgcag accgtctcgc caaactggaa aaatggcaaa cacatctgat taatccacat
      241 atcattctgt ccaaagagcc acaagggttt gttgctgacg ccacaatcaa tacacctaac
      301 ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca ccgcaattaa cgaattgatc
      361 aacaagctgg aacggcagct caataaactg cagcacaaag gcgaagcacg tcgtgccgca
      421 acatcggtga aagacgccaa cttcgtcgaa gaagttgaag aagagtagtc ctttatattg
      481 agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga cagcgtgaaa acagtacggg
      541 tactgtacta aagtcactta aggaaacaaa catgaaacac ataccgtttt tcttcgcatt
      601 cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt gaaacagaat gcgaagacga
      661 acaataaggc ctcccaaatc ggggggcctt ttttattgat aacaaaaagg caacactatg
      721 acatcggaaa acccgttact ggcgctgcga gagaaaatca gcgcgctgga tgaaaaatta
      781 ttagcgttac tggcagaacg gcgcgaactg gccgtcgagg tgggaaaagc caaactgctc
      841 tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt tgctggaaag attaattacg
      901 ctcggtaaag cgcaccatct ggacgcccat tacattactc gcctgttcca gctcatcatt
      961 gaagattccg tattaactca gcaggctttg ctccaacaac atctcaataa aattaatccg
     1021 cactcagcac gcatcgcttt tctcggcccc aaaggttctt attcccatct tgcggcgcgc
     1081 cagtatgctg cccgtcactt tgagcaattc attgaaagtg gctgcgccaa atttgccgat
     1141 atttttaatc aggtggaaac cggccaggcc gactatgccg tcgtaccgat tgaaaatacc
     1201 agctccggtg ccataaacga cgtttacgat ctgctgcaac ataccagctt gtcgattgtt
     1261 ggcgagatga cgttaactat cgaccattgt ttgttggtct ccggcactac tgatttatcc
     1321 accatcaata cggtctacag ccatccgcag ccattccagc aatgcagcaa attccttaat
     1381 cgttatccgc actggaagat tgaatatacc gaaagtacgt ctgcggcaat ggaaaaggtt
     1441 gcacaggcaa aatcaccgca tgttgctgcg ttgggaagcg aagctggcgg cactttgtac
     1501 ggtttgcagg tactggagcg tattgaagca aatcagcgac aaaacttcac ccgatttgtg
     1561 gtgttggcgc gtaaagccat taacgtgtct gatcaggttc cggcgaaaac cacgttgtta
     1621 atggcgaccg ggcaacaagc cggtgcgttg gttgaagcgt tgctggtact gcgcaaccac
     1681 aatctgatta tgacccgtct ggaatcacgc ccgattcacg gtaatccatg ggaagagatg
     1741 ttctatctgg atattcaggc taatcttgaa tcagcggaaa tgcaaaaagc attgaaagag
     1801 ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt acccaagtga gaacgtagtg
     1861 cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg aatcatccgg cactggatta
     1921 ttactggcga ttgtcattcg cctgacgcaa taacacgcgg ctttcactct gaaaacgctg
     1981 tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg tcaataaacg cctgcttatc
     2041 gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta tagtaacgtt tgattaacgc
     2101 cagattacgc tctgacgaca taatgatgtc ggcataaagc tgcggatcct gagcaaacag
     2161 tcgcccgacc atcgccagct caaggcggta aatcggcgaa gagagcgcca gaagttgctc
     2221 aagctgaaca ttttcttctg ccaggtgcag cccgtaagca aaagtagcaa agtggcgcag
     2281 agcctgaata aacgccatat tctgatcgtg ctcgacagcg ctaatacgat gcaaccgagc
     2341 gccccagacc tgaatttgct ccagaaacca ttggtatgct tccggcttac gtccatcaca
     2401 ccagaccaca acttgctttg ccaggctacc gctgtccggg ccgaacatcg ggtgtaaccc
     2461 cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat ggtccatttt tcactgatgc
     2521 cagatcaacc agaatacaat ctttcggtaa aggcggtaat ttgccgataa cttgctcagt
     2581 aacgtggatt ggcacactaa caatcaccat tccggcatcg gcaacaatat cagccgctcg
     2641 atcccagtca tgttgctcca gaatccgcac ctgataaccc gatagtgtca gcatcttctc
     2701 gaacaggcgt cccatctgac cgccaccgcc gacgataacc accggacgca gtgacggaca
     2761 aagtgtttta aatcctttgt cgttttcact ggagtaagat tcacgcatca cccgacgcaa
     2821 aacatcctca atcagatctg gcggtacacc cagagcttcc gcctctgcac gacgcgaggc
     2881 caacatagat gcctcgcgct ccggaacata aataggcagt ccaaagcggc ttttcacctt
     2941 gcccacttca gcaaccagtt ccagacgctt cgctaataaa ttcagcagcg ctttatcgac
     3001 ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc ataataaacc tcttaagcca
     3061 cgcgagccgt cagctgcccg ttcagatcct gatgaatttc acgcagcaag gcatcggtca
     3121 tttcccagct aatgcaggca tcggttacgg atacaccgta tttcatttca ctgcgcggtt
     3181 gctcagaaga ctgattgccc tcgtggatat tactttcgat catcagacca ataattgagc
     3241 gattaccatc tttgatttga gcaaccacgg attctgccac cgcaggctga cggcgataat
     3301 ctttattgga attaccgtgg ctgcaatcta ccatcagaga cgggcgcagt cccgcctgtt
     3361 ccatctcttt ttcacattgc gcaacatccg cagggctata gttcggcgct ttaccaccgc
     3421 gcaggatcac atgcccgtcc ggattcccct gagtttgtag caacgcaacc tgccctgcct
     3481 ggttaatgcc aacaaaacag tgcggctggg cggcggcgcg catagcgtta attgctgttg
     3541 ccagactgcc gtcggtgccg tttttaaaac caaccggcat ggaaagcccg gaggccattt
     3601 cacggtgagt ttgcgattcc gttgtacgag caccaattgc tgaccagcta aacagatcgc
     3661 ccaggtattg cgggctattc ggatctaacg cttccgtcgc cagtggcagt cccatattca
     3721 ccagctcaag caacaattta cgcgcgattt gcagcccggc ttctacatca aaagagccat
     3781 ccatatgggg atcgttaatt aaccctttcc agccgacagt ggtacggggt ttttcaaaat
     3841 agacgcgcat taccagatag aggctatcgc tgacctctgc ggcaagagct ttaaatcgac
     3901 gagcatattc cagagcagtt tccggatcat gaatggaaca aggaccacat actaccagca
     3961 gacgaggatc gcgcccggcg ataatatctg aaatggtttt acgcgagtca gcaatctggg
     4021 cttcttgttg caggctcaat ggaaaagcgg ccttcagttg ttccggagtc attaaaacct
     4081 gttcgtcggt aatatgtacg ttattcagcg cgtctttatg catgatggcg atcctgttta
     4141 tgctcgtttg cgatagttgc tcctcagcga ggatgacgta acgataacac ataaagtaaa
     4201 gttttcaatc catatttcgt acatttttat ttacacaggc aatttagtcg cgctttcaac
     4261 ccttgcttct gtatagataa atttacatcc cctttaaaaa caatccgcta tgctttgaaa
     4321 aaggagaaag aaatgatgaa aaagtttatc gcccccttgt tggctttact ggttagcgga
     4381 tgtcagattg atccttatac tcacgcgcca accttgacca gcaccgactg gtatgatgtc
     4441 ggtatggaag atgcgatatc gggcagcgcc ataaaagatg acgatgcatt tagcgattca
     4501 caggcggatc gcggtctata ccttaaagga tatgccgaag ggcaaaagaa aacttgccag
     4561 accgatttta cttatgcccg gggactttcc ggtaaaagct ttcctgccag ctgcaacaat
     4621 gttgaaaatg ccagccaact gcatgaagta tggcaaaaaa gggctgatga aaatgccagc
     4681 acaatacgat taaattaatt aaaaccccac aaataaatta aacataagat tttactcatt
     4741 gtgttttatt tctcacattg atgacggtcg catgaaaaaa tgatatttta atgagcatcc
     4801 tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga tccctgaatg cgtttttctc
     4861 accgactgtt cttacttctg attctcctgc tgacgggagc accaatactc gctcaagagc
     4921 cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg tattgtcagt tacacccgct
     4981 ggcctgcgct atcagggcca ccaaagttat gtatcttttc atcttcacgt tttagtaccg
     5041 ccctacaaga gaatgccgcg acatctttgc cttatctgcc tgtcatcatt cacactcaac
     5101 aagaggcgat gatttcaggc tgcaatggtt tttattttgg caacgagtca ccgacattcc
     5161 agatggaatt aaccgaacaa tatccgtcaa aggcgttatt attaattgcc gaacaaaata
     5221 ccgagtgcat tattggtagc gccttttgtc tgatcatcca caataatgac gtcagatttg
     5281 ccgtaaacct ggatgcctca tcgcgtagcg gtgtaaaggt caacccggat gtcttaatgc
     5341 tcgcacggaa gaaaaatgat ggataacgat aattctctta ataagcgccc cacgtttaaa
     5401 agagcattac gcaacatcag tatgaccagc atatttatca ctatgatgct gatctggttg
     5461 ctgctttccg tgacctcggt gctgaccctg aaacagtacg cgcaaaaaaa cctggcactg
     5521 acagcagcaa caatgactta cagtctggaa gcagctgtcg tttttgccga tggccctgca
     5581 gcaactgaaa cactggcagc gctgggccag caagggcatt ttcaactgca gaagtacgtg
     5641 ataagcagca aaatattctg gcatcctggc attacccccg taaggatcca ggcgatactt
     5701 tcagcaattt cataagccac tggctcttcc ctgcccccat cattcagccg attcgtcaca
     5761 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
     5821 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
     5881 ccctcacccg ccatttgcac aatggcctgg tggaagcact gaaaaatatc accgatgtcg
     5941 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
     6001 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
     6061 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
     6121 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
     6181 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
     6241 cctggggtca tgcgacgggc gatagggtct tgattgaaat cgcaaaacgg ttagctgaat
     6301 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
     6361 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
     6421 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
     6481 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gcttgccgat cacaatatgt
     6541 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
     6601 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
     6661 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
     6721 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
     6781 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
     6841 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
     6901 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatt ggtggacaaa
     6961 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
     7021 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattgca accccttaaa
     7081 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
     7141 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
     7201 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
     7261 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
     7321 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
     7381 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
     7441 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
     7501 cataatttaa tctcttatcc tgggtaaact gatctctcgg gggcttacgc catcccatca
     7561 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
     7621 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
     7681 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
     7741 tccatccctt ctagcacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
     7801 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
     7861 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
     7921 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
     7981 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
     8041 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
     8101 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
     8161 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
     8221 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
     8281 tcaggaaaca ggctaattat gccaatccac atagcgccgc cttttaccgt ttatccggtg
     8341 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
     8401 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
     8461 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcaac gactttaccg
     8521 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
     8581 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
     8641 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
     8701 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
     8761 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
     8821 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
     8881 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
     8941 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
     9001 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
     9061 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
     9121 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
     9181 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
     9241 tgatttctgg agaaaatgca atcagcagtt aataactctg tcgcataagg tcatttagcg
     9301 accaggaaat cccggcggca tcattccctt catgcttctc atcatcttcg ccattccgcc
     9361 tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac tgcttcagaa ggcggttaac
     9421 gtcctgtacc tgcatcccgc aaccggcggc gatacgacgc ttacgcgaac ctttgatgat
     9481 ttctggctta gcgcgctctt tcatcgtcat tgagttgatg atggcttcca tacgcaccag
     9541 cactttatcg tccatctgcg acttgacgtt atccgggatc tgccccatgc ccggcagttt
     9601 gcccatcaag ctggccatgc cgcccatatt tttcatctgg cgcagttgct caagaaagtc
     9661 gttgagatcg aagccgtcgc ctttttttag cttgctggct aatttctctg cctgcgcgcg
     9721 gtcaactttg ctttcgatat cttcgatcag cgacagtacg tcgcccatgc cgagaatacg
     9781 cgacgcgatg cggtccggat ggaacggctc cagcgcctca gttttctcgc caacaccgag
     9841 gaacttgatc ggtttgccag tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc
     9901 gccgtccact ttggtcaaca ctacgccggt aagcggtaac gcttcattga atgcttttgc
     9961 cgtattggcc gcatcctgac cggtcatggc gtcaaccaca aacagggttt caaccgggtt
    10021 aatcgacgca tggacttgtt tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc
    10081 agcggtatcc accagcagca cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt
    10141 aacaatatca accggcttct gaccaacatc agaagggaag aaatcaacgc ccacctgctc
    10201 tgccagcgtc tcaagctgtt tgattgccgc cgggcgatag acgtcagcag aaaccaccag
    10261 cactttcttc ttgtgcttct cgcgcaggaa attaccgagt ttaccaacgc tggttgtttt
    10321 accggcacct tgcaggcctg ccatcagcac gaccgcaggc ggttgcgcag ccagattcag
    10381 ggtctggttc tcttcgccca tcgccgcaac cagttcgtta cggacgattt tgacgaactc
    10441 ctgccccggc gtcaggctct tattaacttc atgaccaacc gctttctctt ttacgcgatt
    10501 gataaactca cgcactaccg gcagagcgac gtccgcctcc agcagcgcca tgcgcacttc
    10561 gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca cggccactga tattgcgcag
    10621 cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg
    10681 gttggccgca atcgcgacac atcatcagta ttttgccgca gtataacatg aaggcgtctt
    10741 tgttgttatg caacggttgg agcagcgttc acctgacgct atactgcttc tctttcttat
    10801 tgctcaaact gtcgacatta ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc
    10861 cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat
    10921 ggctattatt tctgcggtca ttgcgctggt ctgccacgca atcgctctgg aagcccgcat
    10981 cctgcccgac ggtgatagcg gacaaaacct cagcctgctg aacgttggtt cattggtcag
    11041 tttggtgatc tgtacggtaa tgaccattgt ggcttctcgc aatcgtggct ggttgctgct
    11101 acccattgtc tatgcctttg cgcttatcaa cctggcgctg gcgaccttca tgcccaatga
    11161 atacatcacc catctggaag caacgcctgg gatgctgatg cacattggct tatcgctctt
    11221 ttcctatgcc acgctaatta tcgccgccct gtacgcgctg caactggcgt ggattgatta
    11281 ccaactgaag aacaagaagc tggcatttaa ccaagaaatg ccgccattga tgagtatcga
    11341 gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg ctaacgctca cgctttgcac
    11401 tggcctgttc tacatgcaca acctatttag catggaaaat atcgacaagg ctgtgctctc
    11461 tatcgtggcg tggtttgtct atattgtgct gctgtgggga cattaccatg aaggatggcg
    11521 tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta attctgacac tggcctactt
    11581 cggcagccga attgtccagc agttaatcag ctaaacccag aaaaggagtt tcccctggaa
    11641 cacatttcta ctactacgtt gatcattatt ctgatcatca tggtggtcat ttcagcctat
    11701 ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct atcgtcttcg acatatggcg
    11761 aaacagggta atcgctcggc caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg
    11821 ataagcctgg tgttaatcgg caataacctg gtcaatattc ttgcctccgc gctcggcact
    11881 attgtcggga tgcgtttgta cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact
    11941 tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca ttgccgcgct gtacccggaa
    12001 aaagtcgctt atccgagtag ttttctgctg gctccgctgc aaattttgat gatgccgctg
    12061 gtctggttgc tgaatgctat cacccgtatg ctgatgcgca tgatgggtat caaaaccgat
    12121 atcgtggtta gcggctcttt gagcaaagaa gagttgcgca ctatcgtgca tgaatcgcgc
    12181 tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg tgctcgatct ggaaaaaatg
    12241 accgttgatg acatcatggt accgcgcagt gaaattatcg gtattgatat caacgatgac
    12301 tggaaatcta ttctgcgcca actctcccac tcacctcacg ggcgcatcgt gctttaccgt
    12361 gattcgctgg acgacgccat cagtatgctg cgagtacgtg aagcctggcg gttgatgtcg
    12421 gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg cggacgagat ctattttgtg
    12481 cctgaaggta cgccgctcag cacgcagttg gtaaagtttc agcgcaacaa aaagaaagtc
    12541 ggcctggtcg tcaacgagta tggagacatt caggggctgg tgacggttga agatattctg
    12601 gaagagattg ttggcgattt cactacgtcg atgtcgccaa cacttgccga agaggtcacg
    12661 ccgcaaaacg acggttcggt gattatcgat ggcaccgcca acgtgcggga aatcaacaaa
    12721 gcctttaact ggcatctacc ggaagatgat gcccgcacgg ttaatggcgt cattcttgag
    12781 gcactggaag agatccctgt cgcaggcacc cgcgtgcgta ttggcaagta cgatatcgat
    12841 attctcgacg tacaggacaa tatgattaag caggtaaaag tttttcctgt gaaaccgctg
    12901 cgcgagagtg tggcggagta acgaaaacgg ccctgcattc gcatgcaggg ccgtgaatta
    12961 ttacgaaaac agaaattacg cttttgcttt cgctacagtt accatcgccg cacgaatcgt
    13021 acgaccattc agcgtataac ccttctgcat aatgcccagt acgttacctg gcgcaacgtc
    13081 atcagattcc accattgcga tagcctgatg cacattcggg tccagcggca cgttagtttc
    13141 ggcgatcact tcaacaccaa acttacgcac aacatccagc atcgacttca gcgtcagctc
    13201 aatgccttca accatcgcag acatatccgg gttagcttta tcagccactt ccagcgcacg
    13261 atccaggcta tcaatcaccg gcagcaattc gttgatgaat ttctccagcg cgaatttgtg
    13321 ggctttttca atatccagtt cagtacgacg acgcaggttt tccatttcgg cttttacgcg
    13381 caaaataccg tcacgttcac gggtctgggc ttcagccagc tgagcttcga gattcgcaat
    13441 tttttcatcg cgcggatcca cctgctcagc agaagcttct ggctcaactg cctcaatctc
    13501 ttcgtgctga tccatgataa tttcttccgg ggcttgcccc tcaggcgttt tctgttcttt
    13561 actactcatg aatttctccg cgtttttttc gcattcatct cgctaacttc gcttattatg
    13621 gggatcagtt tcagggtttc aagggaagca ctcacattgt catcaatctt cgcaacaagg
    13681 acctcggaaa aatgaataat catttcaagt gtattggcat tgtgggacac ccacggcacc
    13741 ctactgcact gacaacacat gaaatgctct accgctggct gtgcacaaaa ggttacgagg
    13801 tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa gaatgtgaaa actggcacgc
    13861 tcgcggagat tgggcaactg gctgatctcg cggtagtcgt tggtggcgac ggtaatatgc
    13921 tgggcgcggc gcgcacgctc gcccgttacg atattaaagt tattggaatc aaccgtggca
    13981 acctgggttt cctgactgac cttgaccccg ataacgccca gcaacaatta gccgatgtgc
    14041 tggaaggtca ctacatcagc gagaaacgtt ttttgctgga agcgcaagtc tgtcagcaag
    14101 attgccaaaa acgcatcagc accgcgatta acgaagtggt acttcatcct ggcaaagtgg
    14161 cgcatatgat agagttcgaa gtgtatatcg acgagatctt tgcgttttct cagcgatctg
    14221 atgggctaat tatttcgacg ccaacaggct ccaccgccta ttccctctct gcgggcggtc
    14281 ctattctgac cccctctctg gatgcgatta ccctggtgcc catgttcccg catacgttgt
    14341 cagcacgacc actggtcata aacagcagca gcacgatccg tctgcgtttt tcgcatcgcc
    14401 gtaacgacct ggaaatcagt tgcgacagcc agatagcact gccgattcag gaaggtgaag
    14461 atgtcctgat tcgtcgctgt gattaccatc tgaatctgat tcatccgaaa gattacagtt
    14521 atttcaatac attaagcacc aagctcggct ggtcaaaaaa attattctaa ttttacgcca
    14581 gcctctttac tgtatataaa accagtttat actgtacaca ataacagtaa tggtttttca
    14641 tacaggaaaa cgactatgtt ggcacaactg accatcagca actttgctat cgttcgtgag
    14701 cttgagattg attttcatag cggcatgacc gtaataactg gcgagaccgg tgcgggtaaa
    14761 tctattgcaa tagatgccct cggtctttgt ctcggtggtc gcgctgaagc cgacatggtg
    14821 cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt ctctgaaaga tacgccagcg
    14881 gccctgcgct ggctggaaga aaaccagctt gaagacgggc atgaatgttt gcttcgtcgc
    14941 gtgatcagca gtgatggtcg ctcccgtggt ttcatcaacg gtacagctgt tcctctgtca
    15001 caactgcgcg aactgggtca gttgctgatt cagatccatg gtcagcacgc tcatcaatta
    15061 ctcaccaaac ctgagcacca aaaattcctg cttgatggct atgccaatga aacctctcta
    15121 cttcaggaaa tgaccgcacg ttatcagttg tggcatcaaa gctgccgtga cctcgcgcat
    15181 catcaacagt taagtcagga acgcgccgcc cgtgcggaac tgctgcaata ccaattaaaa
    15241 gaacttaacg aatttaatcc gcagcccgga gagtttgagc aaatcgacga agagtacaaa
    15301 cgtctggcga acagcggtca attgctgacc accagccaga atgcattggc attaatggcc
    15361 gacggtgaag acgcaaacct gcaaagtcag ctttacacgg ctaaacaact ggtgagcgaa
    15421 ttgattggca tggacagcaa actgtccggc gtacttgata tgctggaaga agctaccatc
    15481 cagattgctg aagccagcga cgaactgcgc cactactgcg atcgtctgga tctcgatccc
    15541 aaccgactgt ttgaacttga acagcgcatc tcaaaacaga tttcgctggc acgtaaacat
    15601 cacgtcagcc ctgaggcatt accacagtat taccagtcgc tactggaaga acagcagcaa
    15661 ctggacgatc aggccgactc acaagaaacg ctcgcgctgg cggtaacgaa acatcatcag
    15721 caggcactgg aaaccgcgcg cgcattacac caacaacgcc agcaatatgc agaagaactt
    15781 gcacagctga tcaccgacag tatgcatgcg ctctcaatgc cgcatgggca gtttacgatt
    15841 gatgtcaaat atgacgagca tcacctgggc gctgacggtg ccgatcgtat tgagtttcgg
    15901 gtaaccacca acccaggtca gccaatgcag cctattgcca aagtcgcatc cggtggtgaa
    15961 ttgtcccgca tcgcactggc aatccaggtc atcacggcgc gtaaaatgga aaccccggca
    16021 ctgatttttg atgaagtgga tgtagggatt agcggtccaa cagcggcagt tgtcggcaaa
    16081 ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg ttacccacct gccacaagtc
    16141 gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa ccgatggtgc gatgacagaa
    16201 acacatatgc aatctctgga taaaaaagcg cggttacaag agctggcgcg cctgcttggt
    16261 ggcagtgaag tcacacgtaa cacactggcg aatgcgaaag aactgcttgc agcgtaaact
    16321 tttttcctgc ttcacggtca gagtaaacag caaaacgccg taagaccgga aagcaaaagg
    16381 ttttaaagtg atgaaaggtc tattatcatc ggcatattac agatgagcca cgtactgctc
    16441 gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg ctgactgctg cagcagcagt
    16501 actattgatg ttaaccgcag gctgttccac tctggagcga gtggtttacc gtcctgacat
    16561 caaccagggg aactatctga ccgctaacga cgtatccaaa atacgtgttg gcatgacgca
    16621 acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc gatccatttg gtacgaatac
    16681 ctggttctat gtcttccgcc agcaaccagg tcatgaaggt gtaacgcagc aaacgctgac
    16741 gctgaccttt aacagtagcg gtgtgttgac caatattgat aacaaacctg cgctgagtgg
    16801 taactaataa taaagttgct ctcaaagacg ttaaaaaagg tgctcaatga gcaccttttt
    16861 tctgtctgtt atttattcgc tgatttttct gctcgttgcc tgcgaagctc tttcggatcg
    16921 gcaatgagag ggcgataaat ctccacccga tcgccatcat gcacactatc gcttagtttt
    16981 gccggacggc tgtaaatgcc gactttgttt ttagttaaat cgatatcggt acgcaattcc
    17041 agcaagccac tggcgcgaat agcttcttca accgtcgcgc cctcctgcag cgtcactcgc
    17101 tgcaggtatt gcttctcagg tagcgcataa gccacctcaa cggcaatttt acctagcact
    17161 gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata ttagccgcca gctctttaaa
    17221 cacgcgacca aaggcgagtt caatcaactt attggtaaac tcaaagtcga gatgaaactc
    17281 gatacgacac gcctcctggc tcagcggcgt aaacttccat ccaccaatca atttcttgaa
    17341 cggcccatcc accagattca taagaatact ttggttactg gtcaactggt tgcgggtagt
    17401 aaacgttttg ctgatcccag ccttagagac atctaccgcc gcagtcatct gcccaggagt
    17461 ggactccaga atccgacttc cggtacaacc tggcaaaaac tgaggataag actgaacgtc
    17521 attcactaac tgatacattt gctccgcgct gtagggtacc agtgcggtcc ggctaatctg
    17581 aggcataaca atttccatca acaaaaatcc aacaaataat atcatttatc ctgctgtaaa
    17641 aaaaacgcta tcccggcgct gggtaacatc gggttcatgc taagatagag ccttgtcccc
    17701 cgcaggattg atatggggta ttttcgattt cagattaccg atgattcacg acgcttatga
    17761 cgaagaaaaa agcacataaa cctggttcag cgaccatcgc gcttaacaag cgcgcccgtc
    17821 acgaatactt tatcgaagaa gagttcgaag cgggacttgc cctgcaaggc tgggaagtta
    17881 aatccctgcg cgcaggaaaa gccaatatca gcgacagcta cgtccttctg cgtgacggag
    17941 aggcatttct gtttggcgct aacatcacgc caatggccgt ggcctccacg catgtggtgt
    18001 gcgatcctac ccgtacccgc aagttacttc tcaaccagcg cgaactggac tcattgtacg
    18061 gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc cctgtactgg aaaaatgcct
    18121 ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca gcacgataaa cgttcagata
    18181 tcaaagaacg cgaatggcag gtggataaag cacgcatcat gaaaaacgcc caccgttaaa
    18241 cctgcactcc aattattgac cagttcctca ccgcgcctcc ctctccggcg gcgcgaatga
    18301 acatcttatt ggctatcaca tccgacacaa atgttgccat cccattgctt aatcgaataa
    18361 aaatcaggct acatgggtgc taaatcttta acgataacgc cattgaggct ggtcatggcg
    18421 ctcataaatc tggtatactt acctttacac attggggctg attctggatt cgacgggatt
    18481 tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg taaaaagccg caaaaaatag
    18541 tcgcaaacga cgaaaactac gctttagcag cttaataacc tgcttagagc cctctctccc
    18601 tagcctccgc tcttaggacg gggatcaaga gaggtcaaac ccaaaagaga tcgcgtggaa
    18661 gccctgcctg gggttgaagc gttaaaactt aatcaggcta gtttgttagt ggcgtgtccg
    18721 tccgcagctg gcaagcgaat gtaaagactg actaagcatg tagtaccgag gatgtaggaa
    18781 tttcggacgc gggttcaact cccgccagct ccaccaaaat tctccatcgg tgattaccag
    18841 agtcatccga tgaagtccta agagcccgca cggcgcaagc cctgcgggct tttttgtgct
    18901 ccgaatttgt cccgcgaagt ctgatgccaa ctaattaaat ccgatccttt taggcacctt
    18961 gttaggcact tcataaagct atattgtctt tgaggtgcct aaaactatgg aaacccggca
    19021 atggcaagac aaaccaaacc tctatccgtt aaggaaatcg aatctgctaa acccaaggaa
    19081 gcggactacg ttctctatga tggcgatggc cttgagctac tcattaaatc cagcgggagt
    19141 aaaatctggc agtttcgcta cattcgccct gtcaccaaga cacgagcaaa gaaaagcata
    19201 ggcccttacc cgtcagttac ccttgccgat gccagaaact accgtgcaga gtcccgctca
    19261 ctcctagcta aacagattga tccgcaggaa catcagcaag aacaattgcg tagttcgctg
    19321 gaagccaaaa ccaatacttt ccagctcgta gctgaacgtt ggtggaatgt gaagaaagcc
    19381 agtgtgacag aggactatgc ggaagatatc tggcgctctc ttgaaagaga tgtctttcct
    19441 gcgattggcg acgttagcgt tacagatatt aaagctcata cactggttca ggccgtacaa
    19501 ccggttcagg ccagaggagc actggaaacg gttcgtcgcc tgtgtcaacg tattaatgag
    19561 gtcatgatct atgcccagaa cacagggctg attgatgctg ttcccagcgt taatatcggt
    19621 aaagccttcg aaaagcctca gaaaaagaac atgcccagca ttcgaccgga tcagctatct
    19681 caactgatgc agacaatgcg aacagccagc attagccttt ccacacgctg cctgttcatg
    19741 tggcaacttc ttactattac ccgccctgcc gaagcggctg aagctcgctg ggaagaagta
    19801 gacatagaag cgcaagagtg gaagattccc gcagcacgca tgaaaatgaa ccgcgaccat
    19861 actgttccat tgtcagatga agcaatagct gtgctggaga tgatgaaacc gctaagcggc
    19921 aaccgagaat ttatctttcc tagccgtatc aagcccaacc agcctatgaa cagtcagact
    19981 gttaacgcct cactaaaacg cgcaggtttt ggtggtgtgc ttgtttcgca tggtctgaga
    20041 tcaattgcca gtacggctct taatgaacaa ggctttccgc ctgatgccat tgaggcagca
    20101 ttggcccatg tggataagaa tgaggttcgt cgtgcttata accgcagcga ttacctggaa
    20161 cagcgtcgcc cgatgatgca atggtgggca aattttgtga tggcagcgga tcgtggaagc
    20221 atgattgaag gtgggataaa aggaatgaag ctagttgggt gactaaatag ctgcgcggca
    20281 tagtgtccca ctttcagcca gcccttgcag gttaagccac cagacagtaa acggcgggtg
    20341 tcgcgttatt aggcgcagct atttagtagt aatattagga ttggtgattc tgcactccag
    20401 aagtt
//
LOCUS       DAJTRW010000070        20385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRW010000070 DAJTRW010000000
VERSION     DAJTRW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..168
                     /locus_tag="OO326_002742"
     CDS             <1..168
                     /locus_tag="OO326_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5967077.1"
                     /translation="TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNA
                     IIATLKNVYCP"
     gene            178..702
                     /gene="ygaP"
                     /locus_tag="OO326_002743"
     CDS             178..702
                     /gene="ygaP"
                     /locus_tag="OO326_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5967078.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(749..1153)
                     /gene="stpA"
                     /locus_tag="OO326_002744"
     CDS             complement(749..1153)
                     /gene="stpA"
                     /locus_tag="OO326_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5967079.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1821..2270
                     /gene="alaE"
                     /locus_tag="OO326_002745"
     CDS             1821..2270
                     /gene="alaE"
                     /locus_tag="OO326_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5967080.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2307..2651)
                     /locus_tag="OO326_002746"
     CDS             complement(2307..2651)
                     /locus_tag="OO326_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5967081.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2803..3033
                     /locus_tag="OO326_002747"
     CDS             2803..3033
                     /locus_tag="OO326_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967082.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3380..3625
                     /gene="nrdH"
                     /locus_tag="OO326_002748"
     CDS             3380..3625
                     /gene="nrdH"
                     /locus_tag="OO326_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5967083.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3622..4032
                     /gene="nrdI"
                     /locus_tag="OO326_002749"
     CDS             3622..4032
                     /gene="nrdI"
                     /locus_tag="OO326_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5967084.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4005..6149
                     /gene="nrdE"
                     /locus_tag="OO326_002750"
     CDS             4005..6149
                     /gene="nrdE"
                     /locus_tag="OO326_002750"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5967085.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6159..7118
                     /gene="nrdF"
                     /locus_tag="OO326_002751"
     CDS             6159..7118
                     /gene="nrdF"
                     /locus_tag="OO326_002751"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5967086.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7473..8675
                     /gene="proV"
                     /locus_tag="OO326_002752"
     CDS             7473..8675
                     /gene="proV"
                     /locus_tag="OO326_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5967087.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHVLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8668..9732
                     /gene="proW"
                     /locus_tag="OO326_002753"
     CDS             8668..9732
                     /gene="proW"
                     /locus_tag="OO326_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5967088.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9790..10782
                     /gene="proX"
                     /locus_tag="OO326_002754"
     CDS             9790..10782
                     /gene="proX"
                     /locus_tag="OO326_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5967089.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10974..12153
                     /locus_tag="OO326_002755"
                     /pseudo
     CDS             10974..12153
                     /locus_tag="OO326_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12277..13014
                     /gene="ygaZ"
                     /locus_tag="OO326_002756"
     CDS             12277..13014
                     /gene="ygaZ"
                     /locus_tag="OO326_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5967090.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13004..13339
                     /gene="ygaH"
                     /locus_tag="OO326_002757"
     CDS             13004..13339
                     /gene="ygaH"
                     /locus_tag="OO326_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5967091.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13430..13960
                     /gene="emrR"
                     /locus_tag="OO326_002758"
     CDS             13430..13960
                     /gene="emrR"
                     /locus_tag="OO326_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5967092.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14087..15259
                     /gene="emrA"
                     /locus_tag="OO326_002759"
                     /pseudo
     CDS             14087..15259
                     /gene="emrA"
                     /locus_tag="OO326_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15276..16814
                     /gene="emrB"
                     /locus_tag="OO326_002760"
     CDS             15276..16814
                     /gene="emrB"
                     /locus_tag="OO326_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5967093.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16878..17393)
                     /gene="luxS"
                     /locus_tag="OO326_002761"
     CDS             complement(16878..17393)
                     /gene="luxS"
                     /locus_tag="OO326_002761"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5967094.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17543..19099)
                     /gene="gshA"
                     /locus_tag="OO326_002762"
     CDS             complement(17543..19099)
                     /gene="gshA"
                     /locus_tag="OO326_002762"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5967095.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19172..19600)
                     /locus_tag="OO326_002763"
     CDS             complement(19172..19600)
                     /locus_tag="OO326_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5967096.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19597..20163)
                     /gene="yqaB"
                     /locus_tag="OO326_002764"
     CDS             complement(19597..20163)
                     /gene="yqaB"
                     /locus_tag="OO326_002764"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5967097.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4914 a         5222 c         5096 g         5153 t
ORIGIN      
        1 acgcgcatta ccggactgag cgcctctgcg acatcacagc atctcgctcg tatgcgggac
       61 gaagggctta tcaacagcca acgggatgcc caacgcattc tatattccat taaaaatgag
      121 gcggtaaatg ccattatcgc caccctgaaa aatgtctatt gtccgtaagg agtcactatg
      181 gctttgacaa ccatttcgcc gcatgatgca caagaattaa tcgcacgcgg cgcaaagtta
      241 atcgatattc gtgacgctga tgaatatctt cgtgaacata ttcctgaagc agatctggct
      301 ccattatccg tgctggaaca gtcaggtctt ccacctaaat tacgtcgcga gcaaattata
      361 ttccactgcc aggcaggtaa acgcaccagt aataacgccg ataaattagc agcgattgcc
      421 gcccccgcag aaatattttt actcgaagat gggattgatg gctggaaaag agctggattg
      481 ccagtagcgg taaataaatc tcaacccttg ccgttaatgc gccaggtgca gatcgctgcg
      541 ggtggtttaa tattaaacgg cgttgtactg ggttataccg taaatagcgg tttcttctta
      601 ttaagtggct ttgttggtgc cgggttactg tttgcaggaa tcagcggttt ttgtggaatg
      661 gcaaggttgt tagataagat gccgtggaac caacgagctt gagaagcgac gccggacgcg
      721 ccctagcagc gacatccggc ctcagtaatt agatcaggaa atcgtcgaga gatttacctt
      781 ctgccagcgc ctgagcaatt ggcttcggtg tacggccctg accggtccag gttttagttt
      841 caccgttaac atcagtgaat ttatatttcg ccggacgcgg ctggcgtttt ttaccagcac
      901 gtggtgcagc agcagagcta ttacccaata actcttccgg gttaattccg tcagctttca
      961 tcagctccag ccaggtgcta attttttcct gacgctcagc cagttcacgc tgctgctgtt
     1021 cttcttcttc acgtctttct ttagtgacaa ccctgaattt ttcgagcatt tcttcaagaa
     1081 cgtcaatgga gaattcgcga gccatcgcac ggagggtgcg aatgttattt aaactttgta
     1141 acattacgga cataataaag aaaacctttt aacgccaaaa caaaaaagta tttcgtcgcg
     1201 tagtgtaagc gatcacttat tatatttcca caaaatttaa tctatttaca gtcctgcgcg
     1261 agattatttc agctgttatt ttctgaaact ctcagaataa tttcaagatt atatattagc
     1321 cattttgatg agtaatttag gttattggtg aacagagtgg cgctctctgc tcgctaactc
     1381 cgcaagcctg acaaaaccga agtgaataaa aatgcaatat aacagaataa aagaaaacac
     1441 ccctcactgg tgctttctgc tgttaatgta cattttctgg catcaaaaat gctgtaaatg
     1501 acccggtcat gacaaacacc gaattctcta cgcttatagc gttacctccc ccccaaactt
     1561 tcattagtga aattaaaggc aagatcatat ctaattgatt taattgaaaa taaatcatat
     1621 tggcatgtaa aaatccacaa tgtacaaaaa acgagccgtt acggaatatt ttatctacaa
     1681 aaactaacta aataaaaatt tttcactaat tgattagtca tagacagcga tatacgctat
     1741 gcaaaaatgc agatggcaat gagatccact gctttcatct ccattaacat cccattacgc
     1801 ttttattaag gagcattagc atgttctcac cgcagtcacg cttgcgtcat gcagttgcag
     1861 atacgttcgc gatggttgtt tactgttctg tcgtgaacat gtgtattgaa gttttcctct
     1921 ccggaatgag cttcgaacag tctttttatt ccagattggt agcgattccg gtgaacatct
     1981 taattgcatg gccatacggt atgtaccgcg atctgtttat gcgcgcggca cgcaaagtta
     2041 gcccgtcggg ctggataaaa aatctggctg atatcctggc ttatgtgacg ttccagtcac
     2101 cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg gcatcagatt atggcggcgg
     2161 tcagttcaaa catcgttgtt tcgatgttga tgggggcggt ttatggctac ttcctcgatt
     2221 attgccgccg actgtttaaa gtcagccgtt accagcaggt aaaagcctga ctcttctttt
     2281 cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc caaataaacc attcaaataa
     2341 cgttcaagcg caacacgcga actaaagcca tgcgggatac cgtaatgctc gtgtcgctcg
     2401 cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc ggaagcgggt
     2461 tcagccagtg ttgaactgcg ttcttttaat gttggatgaa ttaccagagc ggtacgtccc
     2521 attcgcgctt cgcgattaac atagacataa ttttccccac ggcggtaacc atacgctttc
     2581 tgggttacca catcgacagt aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt
     2641 cgtaaataca tatttgatcc tcgttatttt caaccgcggg cctaccttac ctgattgcgc
     2701 attcacaacg ctttcagaaa agtccataaa gccgcgatga acctcagtta agaaaattat
     2761 ggtctacact gaaaattaca tcgaattcta atgggggatc atatgtttaa ccgaccgaac
     2821 cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg atgtcaatca attagctgac
     2881 agcctcgaat ctgtattgaa gtcctggggc agcgacgcca aaggggaagc tgaagccgca
     2941 cgcagtaaag ctcaggcgtt gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc
     3001 gtccagcaag ccgcgcgcga tgccgttggc tgagcggatt cttttgttcg tgaaagaccc
     3061 tggtgtagcg tgggtacagc agctgcggta ggtattttta tcggcgcact gttaagcatg
     3121 cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt tgaccccgaa gcctgtcttc
     3181 ggggtttctt tttgcctggt gaatcacaaa aatccccctc ccccgtcacg ctcatatcca
     3241 gggtaatttc gatcactatt tgctatatat tgtgtagttg aatctttttt caactacatc
     3301 tagtatctct gtatcaacag agagacaacc cgacgcgtat catcgcgccg tatcttcatt
     3361 ttaaacggaa atacgaatca tgcgcataac tatttatact cgtaacgatt gcgttcagtg
     3421 ccacgccacc aaacgggcga tggaaaaccg gggctttgat tttgaaatga ttaatgtcga
     3481 tcgcgttcct gaagcagcgg aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt
     3541 gattgctggc gatcttagct ggtctggttt ccgtccggac atgattaacc gtctgcatcc
     3601 agcgccacac gcggccagtg catgagccag ctcgtctact tctccagcag ctccgaaaac
     3661 acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc gcatcccgct caatgagcgg
     3721 gaacggattc aggtagacga gccttacatc ctgatcgtgc cctcttacgg cggcggcggt
     3781 acggctggcg cggtgccacg acaggtaatt cgctttttaa acgacgagca caaccgggcg
     3841 ttgcttcgcg gcgttattgc ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc
     3901 ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt accgttttga actcatgggt
     3961 acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg aattttggca acgacaaccg
     4021 cagaatgcct gacgcaggaa acgatggatt accacgcgct gaatgcgatg cttaatcttt
     4081 acgatagcgc aggtcgcatt cagttcgata aagaccgcca ggccgttgac gcctttattg
     4141 cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca gcagcagcgc ctgaactggc
     4201 tggtcaacga aggttactat gatgaaagcg ttcttaatcg ctactctcgc gactttgtca
     4261 ttacgctgtt tgcccacgca cacaccagcg gttttcgttt ccagacattc ctcggggcat
     4321 ggaagtttta caccagctat acgttgaaga cattcgacgg taaacgttat ctggaagatt
     4381 ttgccgatcg agtcacgatg gtggcgctga cgctggcaca aggcgatgag acgctggcgt
     4441 tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc agccacgcca acattcctca
     4501 actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt tttgctgcgt attgaagaca
     4561 atatggagtc gattggtcgg gcggtaaatt ccgcactgca gctgtcgaaa cgcggcggcg
     4621 gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc gccaattaaa cgtattgaaa
     4681 atcaatcttc tggcgtaatt ccggtgatga aaatgttgga agacgcattt tcctatgcca
     4741 accaactcgg cgcgcgtcag ggggcaggtg cagtctattt acatgctcat catcccgata
     4801 ttctgcgttt tctcgacacg aaacgggaaa atgccgacga aaaaatccgc attaaaacac
     4861 tgtcgctggg cgtggtgatc ccggatatca ctttccatct ggcaaaagag aatgcgcaga
     4921 tggcgctgtt ttcgccctat gacgttgagc gagtttatgg caagccgttt gccgatatcg
     4981 ccattagcga acactatgac gaactggttg ccgatgaacg catccgcaaa aaatacctca
     5041 acgcccgtga tttcttccag cgactggcag aaatccagtt tgagtccggc tatccctaca
     5101 tcatgtatga agacacagta aaccgtgcta accctatcgc cgggcgcata aatatgagta
     5161 atctctgctc agaaattttg caggttaaca gcgcctcaga gtatgacgag aatctcgact
     5221 atgcccgcac aggccatgat atttcctgca atttaggttc gttgaatatt gcgcacacca
     5281 tggattcccc cgatttttcc cgcacggtag agactgccgt gcgcggttta acggcggtgt
     5341 cagatatgag tcatatccgc agcgtgccgt ccatcgaagc cggaaatgcc gcctcgcacg
     5401 ccatcggact ggggcagatg aatttacatg gttatctggc gagggaaggc atcgcttatg
     5461 gttcgccgga agccctggat ttcaccaatc tctatttcta taccatcacc tggcacgcac
     5521 tgcgtacctc gatgttgctg gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt
     5581 cacgctatgc cagtggtgaa tatttcagcc aatatctgca aggtaactgg cagccgaaaa
     5641 cggcgaaagt tggcgaactg tttgcccgca gctgtattac gttacctacc cgtgagatgt
     5701 gggcgcaact gcgcgacgac gtgatgcgct acggcatata caaccagaat ctccaggcgg
     5761 tgccgccaac cgggtctatc tcttatatca accatgctac gtcgagtatt catccgattg
     5821 tggcgaaagt agagatacgc aaagagggca aaacaggacg cgtttactac cctgccccgt
     5881 ttatgactaa cgaaaatctg gcgctgtatc aggacgctta cgaaattggc gcagaaaaga
     5941 tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca ggggctgtcg ctgacgctgt
     6001 ttttccccga taccgccacc actcgcgata tcaacaaagc gcagatttac gcctggcgca
     6061 aaggtatcaa aacgctctat tacatccgcc tgcgtcagat ggcgctggaa ggcactgaaa
     6121 ttgaaggctg cgtctcctgt gcactttaag gaatatctat gaaactctca cgtatcagcg
     6181 ccatcaactg gaacaagata tctgacgata aagatctgga ggtgtggaat cgcctgacca
     6241 gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga tattcccgcc tggcagacat
     6301 taactgtcgt agaacaacaa ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca
     6361 cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga tgcgctcacg cctcatgaag
     6421 aggcggtatt atcgaatatc agctttatgg aagcggttca tgcccgctct tacagttcga
     6481 ttttctcgac gctgtgccag accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa
     6541 acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca ttatcgcggt gatgatccgc
     6601 tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt gttctattcc ggtttctggc
     6661 tgccgatgta tttttccagc cgcggaaagc tgaccaatac cgcggacctg atccgtctga
     6721 ttatccgcga tgaagcagtc cacggttact acataggcta taaatatcag aaaaacatgg
     6781 aaaagatatc tctgggacaa cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg
     6841 aactctacga caacgagttg caatacaccg atgagctgta cgccgaaacc ccgtgggctg
     6901 acgatgtgaa agcgtttctc tgttacaaca ccaataaggc tttgatgaat ctgggctacg
     6961 aaccgttatt tcccgcagaa atggcggaag tgaatccggc aatcctcgcc gcgctttcgc
     7021 cgaatgccga tgaaaatcac gatttctttt ccggttcagg ctcctcttat gtgatgggga
     7081 aagcggttga aacagaagat gaagactgga atttctgagg gtgttatttt caaaaatatc
     7141 actacccgca gcagggaaat aattcccgcc aaatagcttt ttatcacgca aatattctgt
     7201 agtgatctac actgatactc tgttgcatta ttcgcctgaa actacaatat tcaggcgttt
     7261 tttcgctatc tatgacaaaa aatatcaact tttctcgatt tgctctcagc ccttatatca
     7321 cggaaaattc cggcgatttg ctcgcatcaa tattcatgcc acatttgccc tcaggggttg
     7381 cctcagattc tcagtatgtt agggtagaaa aaagtgacta ttcccattgg gtaatatatc
     7441 gacataaaca aataaagaaa tcattctatt gcatggccat taaattaaaa attaaaaatc
     7501 tttataaaat atttggtgag catccacagc gagcgttcaa atatatcgaa caaggacttt
     7561 caaaagaaca aattctggaa aaaactgggc tatcgcttgg cgtaaaagac gccagtctgg
     7621 ccattgaaga aggcgagata tttgtcatca tgggattatc cggctcgggt aaatccacaa
     7681 tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg gcaagtgctg attgatggtg
     7741 tggatattgc caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa aagattgcga
     7801 tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt gctggacaat actgcgttcg
     7861 gtatggaatt ggccggaatt aatgccgaag aacgccggga aaaagccctt gatgcactgc
     7921 gtcaggtcgg gctggaaaat tatgcccaca gctacccgga tgaactctct ggcgggatgc
     7981 gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc ggatatatta ttaatggacg
     8041 aagccttctc ggcgctcgat ccattaattc gcaccgagat gcaggatgag ctggtaaaat
     8101 tacaggcgaa acatcagcgc accattgtct ttatttccca cgttcttgat gaagccatgc
     8161 gtattggcga ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc ggcacaccgg
     8221 atgaaattct caataatccg gcgaatgatt atgtccgtac cttcttccgt ggcgttgata
     8281 ttagtcaggt gttcagtgcg aaagatattg cccgccggac accgaatggc ttaattcgta
     8341 aaactcctgg cttcggccca cgttcggcac tgaaattatt gcaggatgaa gatcgcgaat
     8401 atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc gattcgctta
     8461 aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct gattgatgcg ccgttagcag
     8521 tcgatgcaca aacgcctctt agcgagttgc tctctcatgt cggacaggca ccctgtgcgg
     8581 tgcccgtggt cgacgaggac caacagtatg tcggcatcat ttcgaaagga atgctgctgc
     8641 gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg ggataccacg
     8701 ccagcggcgg acagtgccgc gcaatccgca gacgcctggg gtacaccgac gactgcaccg
     8761 gctgacggcg gtggtgctga ctggctgacc agtacgcctg cgccaaacgt cgagcatttt
     8821 aatattctcg atccgttcca taaaacgctg atcccgctcg acagttgggt cactgaaggg
     8881 atcgactggg ttgttaccca tttccgtccc gtcttccagg gcgtgcgcgt tccggttgat
     8941 tatatcctca acggtttcca gcaattgctg ctgggtatgc cagcgccagt ggcgattatc
     9001 gttttcgctc tcatcacctg gcagatttcc ggggtcggaa tgggcgtggc gacgctggtc
     9061 tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg caatggtgac tctggcgctg
     9121 gtgttaactg ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat atggctggcg
     9181 agaagtccgc gagcggcgaa aattattcgt ccactgcttg atgccatgca gaccacgcca
     9241 gcgtttgttt atctggtgcc aatcgtcatg ctatttggta tcggtaacgt gccgggcgtg
     9301 gtggtgacga tcatctttgc gctgccgccg attatccgtc tgaccattct ggggattaac
     9361 caggttccgg cggatctgat tgaagcctcg cgctcattcg gtgccagccc gcgccagatg
     9421 ctgttcaaag ttcagttacc gctggcgatg ccgaccatta tggcgggcgt taaccagacg
     9481 ctgatgctgg ccctttctat ggtggtcatt gcctcgatga ttgccgtcgg cgggctgggt
     9541 cagatggtac ttcgcggtat cggtcgtctg gatatggggc ttgccaccgt tggcggcgtc
     9601 gggattgtga tcctcgccat tatcctcgat cgcctgacgc aggccgttgg gcgcgactca
     9661 cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg ttggtctgct gacccgccca
     9721 ttcattaagt aactctgcac ttgcccggtg acgccgggca ttatcaccct gccaaaaaaa
     9781 ggaataacaa tgcgacatag cgtacttttt gcgacagcgt ttgccacgct tatctctaca
     9841 caaacttttg ctgccgatct gccgggcaaa ggcattactg ttaatccagt tcagagcacc
     9901 atcactgaag aaaccttcca gacgctgctg gtcagtcgtg cgctggagaa attaggttat
     9961 accgtcaaca aacccagcga agtggattac aacgttggct acacctcgct tgcttccggc
    10021 gatgcaacct tcaccgccgt gaactggacg ccactccatg acaacatgta cgaagctgcc
    10081 ggtggcgata agaaatttta tcgtgaaggg gtatttgtta acggcgcagc acagggttac
    10141 ctgatcgata agaaaaccgc cgaccaatac aaaatcacca acatcgcaca actgaaagat
    10201 ccgaagatcg ccaaactgtt cgataccaac ggcgacggta aagcggattt aaccggttgt
    10261 aaccctggct ggggctgcga aggtgcgatc aaccaccagc ttgccgcgta tgaactgacc
    10321 aacaccgtga cgcataatca ggggaactac gcagcgatga tggccgacac catcagtcgc
    10381 tacaaagagg gcaaaccggt gttttattac acctggacgc cgtactgggt gagtaatgag
    10441 ctgaagccag ggaaagatgt ggtctggttg caggtgccgt tctccgcact gccgggcgat
    10501 aaaaatgccg ataccaaact gccgaatggc gcgaattacg gcttcccggt cagcaccatg
    10561 catatcgttg ccaacaaagc ctggaccgag aaaaatccgg cggcagccaa actgtttgcc
    10621 attatgcagt tgccagtggc cgatattaac gcccagaacg ccattatgca tgacggcaaa
    10681 gcctcagaag gcgatattca gggccacgtt gatggctgga tcaaagccca ccagcagcag
    10741 ttcgatggct gggtgaatga ggcgctggca gcgcagaagt aatttttatt cgggcggata
    10801 aggcgttttc gcctcatccg ccgctctgta cacaatgcct gatgcgacgc tgacgcgtcc
    10861 tatcatgcct acaaaccaca tcgcacattt caacaagcta tctttcatcc catattcatc
    10921 aacatccgct attattgatt tccagcttaa tcatcacctg atgaacaaaa ataatgacta
    10981 aacctaatca tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc accggtctgg
    11041 cggtagccag taactattac gcccagccat tgctcgacac catcgcgcgt aacttttccc
    11101 tttccgccag ttcggcaggc tttattgtta ccgccgcgca gttgggctat gccgcaggtc
    11161 tactgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga
    11221 ccttactggc agcgggcggt atgttaatca ctgccagcag tcagtcactg gcgatgatga
    11281 tcctcggtac ggcattaacc ggtttattct cagtcgtggc acaaattctg gttccgctgg
    11341 cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt atgagcggtc
    11401 tgctgttggg gatattgctg gcacgaaccg ttgccgggtt actggcaaac ctcggcggct
    11461 ggcgcaccgt cttttgggtt gcttcggtgt taatggcact gatggcgctg gcattatggc
    11521 gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg ttgggttccg
    11581 ttttcagcat gttcatcagc gataagatcc tgcgcacccg cgcgttgctg ggctgcatga
    11641 cctttgccaa cttcagcatt ctctggacct caatggcctt tttgcttgcc gctccacctt
    11701 ttaactacag cgatggtgac attggtctgt ttgggcttgc gggagctgcc ggagcgttgg
    11761 gcgctcgtcc ggcgggcggt tttgccgaca agggcaaatc gcaccacaac tttcggtctg
    11821 cttctgctgt tactttcatg gctggcaatc tggtttggtc acacttccgt actggcgttg
    11881 attatcggca ttctggtgct ggacctcacc gtacagggcg tgcatatcac taaccagacg
    11941 gtaatttatc gaatacatcc tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc
    12001 tactttattg gcggtgccgc cggttcgcta atttcagcct cagcctggca acatggcggt
    12061 tgggctggcg tttgtctggc tggcgcgacg attgccctgg ttaacttact ggtctggtgg
    12121 cgaggttttc atcgtcagga agccgcaaat taagcaatca gcaaccgttt tcgtggcttt
    12181 acacttataa gggtgttaag aagcccatca gtctgataag gttaagatat tcattcagtc
    12241 tatttataat attaacaatc gttaagcgta cactctatgg aaagccctac tccacagcct
    12301 gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat tgttattagt
    12361 tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt ctctcctctc
    12421 gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt cattaccgcg
    12481 atgctggcag ccgggagtag tttgtgggtt gctgcactga gcgtcatggc aatggatgtt
    12541 cgccatgttt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg
    12601 aaaaccgccc tatgggcgtt tggcctgacg gatgaggttt ttgccgccgc caccgcaaaa
    12661 ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc cttcagttca
    12721 tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg cttgctgcaa
    12781 ggttatcccg ccgttgaagc tgcattaggt tttatgcttc cggcactctt tatgagtttc
    12841 ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcttt agttggtgcc
    12901 cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat tgtctgtggc
    12961 tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct atgaggttct
    13021 gctgcttggg ttactggttg gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct
    13081 gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc tcgacaccat
    13141 tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag tgatgcacga
    13201 tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca gtttctataa
    13261 aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc tcgcctggaa
    13321 agtgatggcg attatataac ccacaagaat catttttcta aaacaataca tttactttat
    13381 ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa tggatagttc
    13441 gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag attttcctta
    13501 tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc tggagaaccg
    13561 caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt tgattacgct
    13621 ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc ttggatcatc
    13681 ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg
    13741 taaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag gtcacgagtt
    13801 tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt ccgcgctcag
    13861 cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca
    13921 gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa
    13981 tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc ctttttggca
    14041 agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga gcgcaaatgc
    14101 ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct
    14161 ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt ggtttttggt
    14221 actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga ttcaaattat
    14281 gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga
    14341 aggcgatgtt ctggtcactc tcgacccgac agatgctcgc caggcgtttg aaaaagccaa
    14401 aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca gcaaacagtt
    14461 gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg actacaaccg
    14521 ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga
    14581 cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt caacaataca atgccaatca
    14641 ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg ctgccaccga
    14701 agtacgtaac gcctagctgg cgctggagcg tacccgtatt gtcagtccga tgaccggtta
    14761 tgtttcccgt cgtgccgtgc aaccgggtgc gcaaattagc ccaacgacgc cgctgatggc
    14821 ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc agattgccaa
    14881 tatgcgcatc ggtcagccgg tcaccatcac caccgatatt tacggcgatg atgtgaaata
    14941 caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac tgcttccggc
    15001 gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctacctgtgc gtatcgaact
    15061 ggaccagaaa cagctggagc aatacccgct gcgcattggt ttgtccacgc tggtgagcgt
    15121 caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc
    15181 ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg acgatatcgt
    15241 aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag
    15301 gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg caggtgctga
    15361 actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc tcatcgctca
    15421 gccaaggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg atcccgctta
    15481 ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc accatcgcct
    15541 ttgctattgc gtcttgggcg tgtggtgtct ccagcagcct gaatatgctg atcttcttcc
    15601 gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt ctattgctga
    15661 ataactaccc gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg
    15721 tcgcgccaat ttgcggcccg atcctcggcg gttatatcag cgataattac cactggggct
    15781 ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca ctgcaaaccc
    15841 tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg ctggcactgc
    15901 tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag ctggactgtt
    15961 tttcatcaca ggaaattatc attcttaccg tggtggcggt ggtggctatc tgcttcctga
    16021 ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt aagtcgcgca
    16081 acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc ggcgctattg
    16141 ttctgctgcc gcagttgttg caggaggtct acggttacac ggcgacctgg gcaggtttgg
    16201 cctctgcgcc ggtagggatt attccggtga ttctgtcgcc gattatcggc cgcttcgcgc
    16261 ataaactgga tatgcggcgg ctggtaacct tcagctttat tatgtatgcc gtctgcttct
    16321 actggcgtgc ctataccttt gaaccaggta tggatttttg cgcgtcggcc tggccgcagt
    16381 ttatccaggg gtttgcggtg gtctgcttct ttatgccgct gaccaccatt acgctgtctg
    16441 gtttgccacc ggaacgactg gcggcggcat cgagcctctc taactttacg cgaacgctgg
    16501 cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag tcgatgcacc
    16561 atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc
    16621 aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc cagcagatca
    16681 ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc gggatattcc
    16741 tcgtactgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt ggcggcggag
    16801 gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg
    16861 tgaagatagt ttactgacta gatgtgcagt tcctgcaact tctctttcgg cagtgccagt
    16921 tcttcgttgc tgttgatgcg tacctcacgt tccagaatgc tacgcgcaat atcctgcgct
    16981 tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc cgggatctga
    17041 ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc
    17101 tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag
    17161 atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc agcaaacagg
    17221 tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac gcagaagcgc
    17281 agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact
    17341 gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca
    17401 cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata
    17461 tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactcc
    17521 cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg
    17581 tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat
    17641 cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag gcttctgcaa
    17701 acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga
    17761 aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc tgatacgctt
    17821 cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt
    17881 tacccacctg cagtaacggg aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg
    17941 gtttgcgacc ttcaaggatc acccggttcc agttaacgcg cgtacaggca agttcgctac
    18001 tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca
    18061 cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa
    18121 tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc
    18181 gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg ctattgattt
    18241 gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat ggcgttttga
    18301 ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc
    18361 tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt
    18421 aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag
    18481 aagaacaaat cgccggagat gcaccaaaca gataaggaat aacccaaccg aaacgatagt
    18541 aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca gcgcccgaga
    18601 tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg
    18661 aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac agcgttttaa
    18721 agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt
    18781 agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc gtataacgat
    18841 gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt gtaatgaatt
    18901 ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg
    18961 cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct
    19021 ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct
    19081 gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac
    19141 tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg
    19201 tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa aagattactg
    19261 gcccccagga gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca
    19321 tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta gctttctctt
    19381 gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc
    19441 caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggatgactg atcccggaaa
    19501 gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac
    19561 tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacagc
    19621 gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca
    19681 ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt
    19741 atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg
    19801 cgccagcaat gcctcagcga tggcgctttc actccccgtt cctacagcca ttgggcgacg
    19861 accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat
    19921 acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat cggcctgatt
    19981 cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcatcgc
    20041 ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt
    20101 aggcgccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta
    20161 catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt
    20221 cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag
    20281 atatttttga aggaaatttt ttggagaata tggtgcatcc gggaggattc gaacctccga
    20341 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggat
//
LOCUS       DAJTRW010000071        20269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRW010000071 DAJTRW010000000
VERSION     DAJTRW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO326_002765"
     CDS             complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO326_002765"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5967098.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO326_002766"
     CDS             complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO326_002766"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5967099.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1718..2990)
                     /locus_tag="OO326_002767"
                     /pseudo
     CDS             complement(1718..2990)
                     /locus_tag="OO326_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3173..4378)
                     /locus_tag="OO326_002768"
     CDS             complement(3173..4378)
                     /locus_tag="OO326_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967100.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4478..4834)
                     /locus_tag="OO326_002769"
     CDS             complement(4478..4834)
                     /locus_tag="OO326_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5967101.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4970..5077)
                     /gene="rprA"
                     /locus_tag="OO326_002770"
     ncRNA           complement(4970..5077)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO326_002770"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5263..6375)
                     /gene="ydiK"
                     /locus_tag="OO326_002771"
     CDS             complement(5263..6375)
                     /gene="ydiK"
                     /locus_tag="OO326_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5967102.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6764..9820
                     /locus_tag="OO326_002772"
     CDS             6764..9820
                     /locus_tag="OO326_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5967103.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9817..10227
                     /gene="menI"
                     /locus_tag="OO326_002773"
     CDS             9817..10227
                     /gene="menI"
                     /locus_tag="OO326_002773"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5967104.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10327..10515
                     /locus_tag="OO326_002774"
     CDS             10327..10515
                     /locus_tag="OO326_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5967105.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11063..11431
                     /gene="sufA"
                     /locus_tag="OO326_002775"
     CDS             11063..11431
                     /gene="sufA"
                     /locus_tag="OO326_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5967106.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11440..12927
                     /gene="sufB"
                     /locus_tag="OO326_002776"
     CDS             11440..12927
                     /gene="sufB"
                     /locus_tag="OO326_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5967107.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12937..13683
                     /gene="sufC"
                     /locus_tag="OO326_002777"
     CDS             12937..13683
                     /gene="sufC"
                     /locus_tag="OO326_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5967108.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13658..14929
                     /gene="sufD"
                     /locus_tag="OO326_002778"
     CDS             13658..14929
                     /gene="sufD"
                     /locus_tag="OO326_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5967109.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14926..16146
                     /gene="sufS"
                     /locus_tag="OO326_002779"
     CDS             14926..16146
                     /gene="sufS"
                     /locus_tag="OO326_002779"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5967110.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16159..16575
                     /gene="sufE"
                     /locus_tag="OO326_002780"
     CDS             16159..16575
                     /gene="sufE"
                     /locus_tag="OO326_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5967111.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16724..17728
                     /gene="ldtE"
                     /locus_tag="OO326_002781"
     CDS             16724..17728
                     /gene="ldtE"
                     /locus_tag="OO326_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5967112.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17791..18027)
                     /gene="lpp"
                     /locus_tag="OO326_002782"
     CDS             complement(17791..18027)
                     /gene="lpp"
                     /locus_tag="OO326_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5967113.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18338..19750)
                     /gene="pykF"
                     /locus_tag="OO326_002783"
     CDS             complement(18338..19750)
                     /gene="pykF"
                     /locus_tag="OO326_002783"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5967114.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5308 a         4860 c         5173 g         4928 t
ORIGIN      
        1 tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc ttatgcctga
       61 tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg cgcagaggct
      121 ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag acgagaaatt
      181 acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg ctcctgcatc
      241 tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc
      301 ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct
      361 tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg
      421 taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc gatcatatca
      481 accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc
      541 tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc tggcatggtt
      601 tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc ggcaaagtgg
      661 tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc
      721 acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc cgcaccaatg
      781 acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt
      841 caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat
      901 aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa
      961 ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg
     1021 accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt ctcaaggggt
     1081 ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc ttcagcaaag
     1141 gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc gttaacccgc
     1201 tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct
     1261 tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac
     1321 agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc
     1381 gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt gttgatggca
     1441 cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc
     1501 ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat tgctccggga
     1561 aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc tttttctaag
     1621 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caatccaatc
     1681 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
     1741 ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga ataataaaaa
     1801 cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
     1861 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
     1921 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
     1981 tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
     2041 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
     2101 cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc cggaccattt
     2161 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
     2221 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
     2281 acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
     2341 cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc tacgctggcg
     2401 tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaacaga
     2461 atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat atagctcacc
     2521 agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat gaccgccgaa
     2581 ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag
     2641 ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg gcaagcggga
     2701 ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca
     2761 aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa accgataccg
     2821 gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag agatgtcata
     2881 ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta aatacaaaga
     2941 acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat tgttctccga
     3001 cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat
     3061 gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc
     3121 agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac
     3181 ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga
     3241 taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg gcaatacttc
     3301 tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgtactgt
     3361 aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt aacgtcaggc
     3421 caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat attatcacca
     3481 cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata aaggtgtaca
     3541 gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta ataaatacac
     3601 aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg tatgacatgc
     3661 ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg
     3721 agatatagcc atataaggta taactggcta aatcgataat tgaacagcga tgttccgtag
     3781 agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg aacgtacaac
     3841 ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag gaccaaccga
     3901 accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat tgtccgctgg
     3961 aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa gcttccatca
     4021 aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa
     4081 cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc atatagcagc
     4141 acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa
     4201 gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca cccgcggcat
     4261 tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac
     4321 catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga tttttcatag
     4381 gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta tccattcatt
     4441 attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc tccagatcgt
     4501 gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa ttgccatcag
     4561 ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc
     4621 ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga cgcatagcca
     4681 acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac tgacgccagc
     4741 tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa
     4801 tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat gaaataaaca
     4861 ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac agtatcttgt
     4921 gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag
     4981 cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac tcaggggatt
     5041 tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag gcatacggac
     5101 tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt tgcgacaggt
     5161 actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa atatcaatta
     5221 gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat tcggtttctc
     5281 gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg ggacttcttc
     5341 cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa taaacagacc
     5401 gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga tcagcggtaa
     5461 atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc caaccacacc
     5521 gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga gccagataat
     5581 cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga tcattaacac
     5641 cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc caagcactgc
     5701 ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct gtgccgccag
     5761 cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc gaatgccttg
     5821 tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca gcgcacaatg
     5881 caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg tggtgccaat
     5941 ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt tgtgccagcc
     6001 tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat cgggtaacgt
     6061 catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga tactgttaac
     6121 cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca ccagaacggc
     6181 gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc aggtggcgat
     6241 aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc acagacatgc
     6301 cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat ccctgggctg
     6361 acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt
     6421 gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta acaggccgat
     6481 tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca
     6541 cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt tcgttcaccc
     6601 cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc tgcaactcga
     6661 aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc tgaaagaata
     6721 gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc cacagatttc
     6781 cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg agcaacaagg
     6841 ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga ccgacaacag
     6901 tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag atgtggcgct
     6961 gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc cccgcggcgg
     7021 cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata tgtcccgcca
     7081 tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg aggccggggt
     7141 gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg cgccggaact
     7201 ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat ccggtcaggg
     7261 atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg tgttgttggg
     7321 gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc tgggtaaatc
     7381 caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc agcaacgcca
     7441 gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg atctgcgtca
     7501 tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg gttcagaagg
     7561 gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta aagtgcgccg
     7621 tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc cgtttatggt
     7681 tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc tggcgcggga
     7741 agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca aagagatgct
     7801 cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg agcgagttaa
     7861 tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg tgattggctg
     7921 gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca aaaaagccgt
     7981 tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg aggatacctg
     8041 cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc tcgacagcca
     8101 cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg tccgtccggc
     8161 actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg atgacgtggt
     8221 ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc
     8281 tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca aagtgaaagc
     8341 ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag aaggtctcga
     8401 tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc agatccccat
     8461 tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa
     8521 ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc ggattcattc
     8581 accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg atcgcggcgt
     8641 tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc ggacgttaat
     8701 tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt
     8761 caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt gccccatcaa
     8821 aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc gttatttacg
     8881 cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga tggcacgcgc
     8941 gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct cggaaaaaca
     9001 tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac aaatggtggg
     9061 gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag agcagaaagc
     9121 gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc aagtggtggc
     9181 ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc cattttcgcc
     9241 aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga cggcgaaaaa
     9301 gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg gcgtcgatcc
     9361 ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg aacgtggcga
     9421 gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac atccagtggc
     9481 tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta ccgctttgcc
     9541 gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt
     9601 cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa accatgaaaa
     9661 ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc cgcgtaaccg
     9721 ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag gcacgggggt
     9781 acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg gaaaatcacc
     9841 ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga tattcgcttt
     9901 gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg gacaaagcag
     9961 cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat cggttccgtt
    10021 gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat caatgctaat
    10081 cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt gcatctcggt
    10141 tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt gtgctgttcg
    10201 tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac gcatattgct
    10261 tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc taaaagcgag
    10321 gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt acgccgtgac
    10381 cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga acttgaattt
    10441 gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt cgcctggcgt
    10501 ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc tggattaacc
    10561 tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata ttgttcaaaa
    10621 ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc
    10681 gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac
    10741 ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt
    10801 acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg
    10861 ctccttagat aataaccatt atctaacaat gagataccta attcttagcg tgcctgttaa
    10921 cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt acggtaaagc
    10981 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
    11041 agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc cacaggattt
    11101 cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg agctggtggc
    11161 aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct gcgcgggctt
    11221 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
    11281 cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca cggaagtcga
    11341 tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
    11401 atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa tactgaagca
    11461 actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg attcttcacc
    11521 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
    11581 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta tcgcgcatgg
    11641 ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
    11701 tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc gtctgaacct
    11761 ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
    11821 gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac
    11881 tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat tattttctgt
    11941 tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
    12001 gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc tgacggtacg
    12061 tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgcatt
    12121 aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga agacagctac
    12181 gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt gcacgcggca
    12241 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
    12301 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc
    12361 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
    12421 aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
    12481 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
    12541 accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
    12601 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca
    12661 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
    12721 agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
    12781 tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc
    12841 aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
    12901 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
    12961 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
    13021 gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc
    13081 gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa agatttgctt
    13141 gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca gtatccggtg
    13201 gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc agtgcgcagc
    13261 tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
    13321 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
    13381 ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt
    13441 cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc cgatggcgtg
    13501 aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca acgcattctc
    13561 gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt gaaatccggc
    13621 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
    13681 taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctcaccgca
    13741 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
    13801 ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca ttgcgggaga
    13861 tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc ggctggtgtt
    13921 tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg gatatgaagt
    13981 gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag tgtttctgca
    14041 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
    14101 ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag aggtgaacac
    14161 tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
    14221 ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta tcaatgtcgc
    14281 agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca gtcaccactt
    14341 tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata gtttcctgct
    14401 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct
    14461 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct
    14521 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
    14581 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
    14641 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata caaaaccgca
    14701 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
    14761 tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
    14821 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
    14881 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc
    14941 gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt gccgctggct
    15001 tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc cgaggccgag
    15061 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaggcg
    15121 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    15181 gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    15241 ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct
    15301 aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatccct
    15361 ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga tgagaaaact
    15421 cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc actggcggaa
    15481 atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg
    15541 atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt ctccgggcat
    15601 aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt gttgcaggag
    15661 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
    15721 acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
    15781 ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat agccgagtat
    15841 gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga tctcactctc
    15901 tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
    15961 gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca tcactgcgct
    16021 atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct ggccatgtat
    16081 aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca ccgtctgctg
    16141 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
    16201 ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg ccagcgtctg
    16261 ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg ccagagtcag
    16321 gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg cgacagcgat
    16381 gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg
    16441 caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat
    16501 ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    16561 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg
    16621 tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat aagcaccatc
    16681 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt
    16741 gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta
    16801 tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcaaga
    16861 aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa tgttgatcct
    16921 tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa ctattccttc
    16981 acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg cagagctgcg
    17041 cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag gtattggatt
    17101 gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc ctaacccaac
    17161 ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta aattaccgcc
    17221 agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg
    17281 taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt tgcgcgtcag
    17341 ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac
    17401 gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac ctaacgggat
    17461 gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg ttcagacaat
    17521 gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa
    17581 gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga gcagtggagc
    17641 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
    17701 agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa tggcgcacaa
    17761 tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc
    17821 cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc
    17881 gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag
    17941 ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc
    18001 gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt taatctccat
    18061 gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt gatgggaagg
    18121 cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag ataaaaaaat
    18181 tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat tatatagtga
    18241 aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag cgcccatcag
    18301 ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga acagatgcgg
    18361 tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg tcacctttgt
    18421 gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca tcagtagaag
    18481 tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga tgagccgttt
    18541 tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt actgcgcgag
    18601 cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt tcagcagttt
    18661 caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca ttgttgaact
    18721 cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg atagaaaccg
    18781 cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg tcagtaccgt
    18841 cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg tttttgatca
    18901 tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata catttttcga
    18961 tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc aggtcgccac
    19021 gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg aggccttcct
    19081 ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc aggtgctcac
    19141 ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag tctacgcctt
    19201 tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc agagcaatgg
    19261 aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc agcactttac
    19321 agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca tcgtcaacca
    19381 gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc gcaaccattt
    19441 cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca gctttcagag
    19501 aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg gtatcaagca
    19561 ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga atgcgctgac
    19621 cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg ccagcgtcca
    19681 gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa acaattttgg
    19741 tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact tgctttctgg
    19801 gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc tctaaggata
    19861 ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca acgtgagtgt
    19921 ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa gagtcaattg
    19981 gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt tcaaaaaata
    20041 tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga aaaggttacg
    20101 caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga ggataagtga
    20161 aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa taatttatcg
    20221 cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgat
//
LOCUS       DAJTRW010000072        20191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRW010000072 DAJTRW010000000
VERSION     DAJTRW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..507
                     /locus_tag="OO326_002784"
     CDS             <1..507
                     /locus_tag="OO326_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5967115.1"
                     /translation="GRLFREGFSNYDPIGVLNAMASQRAKEARGGELQINELLPASLD
                     AAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPV
                     MVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALAKVI
                     PFFNTHLG"
     gene            525..1163
                     /locus_tag="OO326_002785"
     CDS             525..1163
                     /locus_tag="OO326_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5967116.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1454..2182
                     /gene="mpaA"
                     /locus_tag="OO326_002786"
     CDS             1454..2182
                     /gene="mpaA"
                     /locus_tag="OO326_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5967117.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2157..3122)
                     /gene="ycjG"
                     /locus_tag="OO326_002787"
     CDS             complement(2157..3122)
                     /gene="ycjG"
                     /locus_tag="OO326_002787"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5967118.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3241..3747
                     /gene="tpx"
                     /locus_tag="OO326_002788"
     CDS             3241..3747
                     /gene="tpx"
                     /locus_tag="OO326_002788"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5967119.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3791..5332)
                     /gene="tyrR"
                     /locus_tag="OO326_002789"
     CDS             complement(3791..5332)
                     /gene="tyrR"
                     /locus_tag="OO326_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5967120.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5480..6541)
                     /locus_tag="OO326_002790"
     CDS             complement(5480..6541)
                     /locus_tag="OO326_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5967121.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6538..7935)
                     /locus_tag="OO326_002791"
     CDS             complement(6538..7935)
                     /locus_tag="OO326_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5967122.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8091..9089
                     /locus_tag="OO326_002792"
     CDS             8091..9089
                     /locus_tag="OO326_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5967123.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9200..10072)
                     /gene="ompG"
                     /locus_tag="OO326_002793"
                     /pseudo
     CDS             complement(9200..10072)
                     /gene="ompG"
                     /locus_tag="OO326_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10145..11226)
                     /locus_tag="OO326_002794"
                     /pseudo
     CDS             complement(10145..11226)
                     /locus_tag="OO326_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11240..11899)
                     /gene="pgmB"
                     /locus_tag="OO326_002795"
     CDS             complement(11240..11899)
                     /gene="pgmB"
                     /locus_tag="OO326_002795"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5967124.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11896..14163)
                     /gene="ycjT"
                     /locus_tag="OO326_002796"
     CDS             complement(11896..14163)
                     /gene="ycjT"
                     /locus_tag="OO326_002796"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5967125.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14160..14783)
                     /locus_tag="OO326_002797"
     CDS             complement(14160..14783)
                     /locus_tag="OO326_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5967126.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14780..15202)
                     /locus_tag="OO326_002798"
     CDS             complement(14780..15202)
                     /locus_tag="OO326_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5967127.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15224..15999)
                     /locus_tag="OO326_002799"
                     /pseudo
     CDS             complement(15224..15999)
                     /locus_tag="OO326_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16017..17069)
                     /locus_tag="OO326_002800"
     CDS             complement(16017..17069)
                     /locus_tag="OO326_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5967128.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17100..17942)
                     /locus_tag="OO326_002801"
     CDS             complement(17100..17942)
                     /locus_tag="OO326_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5967129.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17929..18810)
                     /locus_tag="OO326_002802"
     CDS             complement(17929..18810)
                     /locus_tag="OO326_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5967130.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18831..20114)
                     /locus_tag="OO326_002803"
                     /pseudo
     CDS             complement(18831..20114)
                     /locus_tag="OO326_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         4903 a         5542 c         4935 g         4811 t
ORIGIN      
        1 gggcgtcttt ttcgggaagg tttcagcaat tatgatccga ttggcgtatt gaatgcaatg
       61 gcgtcacaac gagcaaaaga ggctcgtggt ggagaattac agatcaacga attacttccc
      121 gccagcctgg atgcagcaaa agcccatgga ctgaccgaac gcgatgttta tgaagcaact
      181 gactactata aaacaccacg cgggcaacaa ccgggaggtg cgacaaaaat gttgttctca
      241 catgcacaga aaacgctggc ctgggatgcc ttcgcattta ctgaagtgtt actgacccag
      301 ccggtgatgg tagtagttgg tgaaaaagtc ggtgccttcg gtgcttatcg tgatggactg
      361 gaagtctatg gccgggcgat ggtttcgcag gatcgtcagt tagtttcgtt acccgatttc
      421 tcacattatg aactatatga caaaccggaa gcagttcagg aggcgctggc aaaagttatt
      481 ccttttttca acacccatct gggataagtt tcatgagaaa tattatgaaa aatgtactga
      541 ttcttggtgc tggcggtcag atagcccgcc atgtgattaa tcaactggca gataaacaaa
      601 ctattaaaca aactttattt gcccgtcagc cagcaaaaat acacaaaccc tatccaacta
      661 atagccagat tattatgggg gatgtactga atcatgcagc actgaagcag gccatgcagg
      721 gacaggatat tgtgtatgcc aaccttacgg gggaagattt agatattcag gcaaattcag
      781 tgatcgctgc gatgaaagcc tgtgatgtta aacgtctgat ctttgttctt tccctcggta
      841 tttatgatga agttccagga aaatttgtcg aatggaataa tgcagttatt ggtgaacccc
      901 taaaaccatt ccgcagggct gcggatgcaa ttgaggcttc tggactggaa tatacaattc
      961 ttcgccctgc ctggctgact gatgaagata ttattgatta tgagcttacc tctcgcaatg
     1021 aaccattcaa aggtacgatc gtatcacgta agagtgtcgc agcattaatt acagatatca
     1081 tcgataaacc tgagaaacac attggtgaga atataggcat caatcaaccc gggactgatg
     1141 gcgataagcc attcttcatg taaatcaatt atatagatac catcgtactg tactatgtgc
     1201 agtacgattt attaaactta ttaaggtgaa ctgatcaatc agatgcgatt aacatgtgta
     1261 gaaccagtaa gaataaaata cgccatgtat ataacattac gacaattcta atatctgcac
     1321 atcaatgaaa atgcaaacaa cttccggcaa aaaagtgata caagatcgat gacatagcgt
     1381 tgaagaaaag gatttgataa tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga
     1441 accagagacg atcatgaccg taacccgctc acgcgccgaa cgcggcgcat ttccgcccgg
     1501 aacagaacat tacggacgtt cattattggg cgcgccgtta atctggtttc ctgcccctgc
     1561 agccagccgc gaaagtggtt tgattctggc tggcactcac ggtgatgaaa actcttcggt
     1621 cgtcacgctc tcctgtgctc tgcggacttt gacaccttct ttgcgccgcc atcatgtggt
     1681 gctgtgtgtg aatcctgacg gctgccagct ggggttacgg gccaatgcta atggtgtgga
     1741 tttaaacaga aactttccgg cggcgaactg gaaggaaggt gaaacggttt atcgctggaa
     1801 cagcgccgct gaagaacgtg atgtcgtatt actgacaggt gacaaacctg gctccgaacc
     1861 tgaaacccag gcgctgtgcc agcttataca ccgcattcaa cctgcctggg tggtctcatt
     1921 ccacgatcca ctggcttgta ttgaagatcc cagacacaac gaattaggcg aatggctggc
     1981 ccagtcgttt gaattgcctc tggtaaccag cgtcggttat gaaacgcctg gctctttcgg
     2041 cagttggtgt gcagatctca acttgcattg catcaccgca gagtttccgc ccatctcctc
     2101 cgacgaagcc agcgaaaaat atctctttgc gatggcaaac ttgctgcgct ggcatcctaa
     2161 agatgcaatt cgcccgtcgt gaactgaagc gccggttcca catctaccgc cagccaggtc
     2221 ggtccgtcaa gatcggcgaa actgacctgc ggcaccagcg gtaaagcggc gctaatggca
     2281 cgagaggtac acaacatgca gcccagcatc agactgaaac cttgtgcacg cgcttcagtc
     2341 gccagcgcca gcgcttccgt cagaccaccg gttttatcga gcttaatgtt aaccatctca
     2401 tagcgccctt tcagcgcctt cagattgcta cgagtatgac aactttcatc agcacaaatc
     2461 ggcaacggat gaataaaatt ctccagcgcc gcatcgtcct gcgcaggaag cggttgttca
     2521 agcatcgcaa cgcctaaatc cgccaatagc tggcaacgcg ccgccaaccc ttctgcacgc
     2581 caggattcat ttgcatcaac gatcagcgtg gcatcgggca cagcggtgcg aattgccacc
     2641 atccgctcac tgataagatg gttatccagc ttcactttca gtaatttcgc gcctgcctgc
     2701 cagagtgttg atgcactatt ggccatctga tcaggcgtac cgatgacaac cgtctgtgca
     2761 gtgataactg tctcgggaag cgttatgccg atcaaatcag ccagcgattg ctgctgtttt
     2821 cgcgccgcga gatcccacaa tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga
     2881 attttttgca actcctcccg tgtcagccct ttctctaatt gcggcacgac gctcataatt
     2941 tgcgccatta ccgaggcatc actttctcca taacgcggat acggcgtgca ttcgccggtg
     3001 cctttaatac cctcttcttc cagttcaacc accaccacgc gcgcttcact gcgacttccc
     3061 cgggcaatca caaacggggt atgtaagggc caggcttcct caaatacctt aacgattctc
     3121 atctttactc cttgttgtcg cggtggtctg cggattaagc gcaaatagcg tttgctgtgt
     3181 tattgacagt tagcataaac taggtgtgac gttaactata tgtaaacagg aaagataatc
     3241 atgtcacaaa ccgttcattt ccagggcaac ccggttacag tcgccaattc catcccgcag
     3301 gcgggtagca aagcgcagac ttttactctc gtggcaaaag atctgtctga cgtcaccctc
     3361 ggtcagtttg cgggtaaacg caaagtgctg aacattttcc cgagtattga taccggtgtt
     3421 tgcgccgcat cagtacgtaa gtttaaccaa ctggcaactg agatcgacaa caccgttgtg
     3481 ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg
     3541 aacaacgtta tcaccctctc cactttccgt aacgctgaat ttctgcaagc ctacggtgtg
     3601 gcaattgctg atggcccact gaaaggtctg gcagcgcgtg ccgttgtggt tattgacgaa
     3661 aatgacaatg tgattttcag ccagctggtg gatgaaatca ccaccgagcc ggattacgaa
     3721 gcagctctgg ctgtactgaa agcataagtt aatatgcctg atggtgttgc accatcaggc
     3781 atattcgcgc ttactcttcg ttcttcttct gactcagacc atattcccgc aacttattgg
     3841 caatcgcggt atgtgaaacg ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc
     3901 gataaagctg ggttaatacc gagcgttcaa aacggctggt gatttcgtcc agcgaacctt
     3961 ccatcgcatc ttcgcccacg gctaccgttg cggcgtcata atccggcaac aaaatatcct
     4021 gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg atagatagcg ttctttaact
     4081 gccgcacatt tcccggccac gcataacgtg taagtacagt attcaggtca gcggccagtt
     4141 tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac gaacagctca gttaacggca
     4201 taatgtcctg cggacagtca cgtagcggcg gcagattgag cgtcaacacg ttcagacgat
     4261 aatagagatc ttcacggaac acgccttttt gcaccagttc gaccagattc ttctgcgtag
     4321 cgcaaatcac ccgcacatcg acatgcacct catggtcttc gccaacccga cggaaagtgc
     4381 catcattaag aaaacgcagt aatttcgcct gcatccgtgg tgacatttcc cctatttcat
     4441 ccaacagcac cgaaccaccg ttcgcctgct caaagaaccc tttcttccct tccggagcat
     4501 gaccaaacag ttcactctcg accgcatctt ctggtataga cgcgcaattc agcgccaggt
     4561 aaggtttgcc cgctctgggg cttgcctgat ggcaggcgta ggcaaaaaga tctttacctg
     4621 tacctgtgtc acccgtaatc agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt
     4681 cgacaacgtg cttcattttc gggctgacgg cgacaatttg gctgaaggcg ctgacgtcct
     4741 gggcggcgac attttgcaac tggcggccca tacgaatcgt tgatcgcaac atcaccaccg
     4801 caccggtcag gacgtgttga tcattttcat cctgaagata aacaggcgta atctccatca
     4861 ggaaattctg cccattaata acgacatgct cgttatgcga atcttgcggt tcgctttcca
     4921 gccaacgtaa aaaattaaag ccgttaatca attgtgcggc ggtatggttg cgcaggcgat
     4981 ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc catatccact ttgcttttca
     5041 tatcgacaga gagcacaggt tcaggcaacg cctccagtaa cgcgctcaac gccagatgct
     5101 cacgttcgga aggcatccac gggacagtac gcacatcggt aacacctgca atacggcgta
     5161 tttcggccat cagactgctg aaactctcaa actccagttc agcaaaattg aggtagattc
     5221 gcccaatggg atcaatctca ataccgcgta aatcaatgcc tcttagcacg agtagatcga
     5281 gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac ttccagacgc atgggaacct
     5341 tcacctgaaa aaagaacaat taatatgatg atatgacact atttggatag caggaaggtt
     5401 tctgtcaaca atcattgaca caaagacgga aaaacgggca cttcggcgta aagatatccg
     5461 gtcagcaccc gatattgcat tatttttcgc tgggcgtttt gcctttttgc agagtttctt
     5521 tcacctgacc gataagctga cgacggaaat ccccaaggcg aggtttgtca tcgtcaatcc
     5581 acggcagcgg gcggcaaagc tccatagctt taatcccgag tcgtaccgtc agaagtccag
     5641 caccaattcc ctgagctgcg cgggtagaca aacgagcagc gagatcttgc gacatccagt
     5701 ccatccccac ttcgcgcacc agttcgctgg ctccggcaaa agcgatattc agcaatacca
     5761 gcttaaacag acgcaaacgg ctgtaatacc ccagttcaat gccatacagc gtggcaatgc
     5821 gattaattaa acgcagattg cgccaggcga taaacgccat atcgaccaac gccagcgggc
     5881 tgaccgcaat catcaacgtt gattccgccg ccgaacggct gatttcgcgc cgcgcctggg
     5941 catctaaaac tggctggacc aaatgcgcat acaggctgac cacttcacga tcgttctgcg
     6001 tttcatggat tgaggcatac cagcgttgca gcgccggatg cgactgatca atacccgcct
     6061 gctgcgccag tttttcgcaa aatgcgcgac ctttgcccgt gccgtggcta tgcaataaat
     6121 cacgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg
     6181 ttaccacaga acctacgcca gcgccgataa tcaatgcccc agcggcacat ccacccagcg
     6241 ctacccagtc ctgggtttgc caggcattca ttgtccactg tataccctgc ccgacaacgc
     6301 ttgcgccaaa cagagccagc ccgcccatca ccattttgcg ccacaggctg cgtttcggac
     6361 gtaacgctgc gtccattacc gcttcgactt gcccctcttc ttcctgcgct tcgtcgagcg
     6421 tggccggggc aaaattttgc gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg
     6481 gattctgttc gacctccagc ggaccgtcga aatcaatacg tggttttaac ggttcggtca
     6541 tcgcaattta tctcctatta aaaattccag cgcggcatca agacgaatat gcggcagcgg
     6601 tttgtcgaca tccatcacct gcgggcgaaa cgcttcaaac tggaaccctt gcttgtccca
     6661 gaacgcctga ccgggcaaac gcgcaggaac ttcaccgggg taaacggtga gaagcgcacc
     6721 atcgctgagc cggttaccgc gcaatgccgg aattttctcg ccgttgacgt caataatgcc
     6781 gctggtggtc gcctgcaccg aggccagccc caggcaatcc atactaatcc tttcaaacgc
     6841 cgcattttgc caggcatcct gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg
     6901 atcgaccgtt acatggtccg ctttcatcgc ggcaaacagc agtttgtcga taaccggcga
     6961 gaataagcgg cgaaacagcg tgcgctgccc gtagtggaaa ctttgcatca gctgcgtcag
     7021 tgccagacgc atatcattaa atgcctgtgg cccactgttg agaggttgca ggcaatccac
     7081 cagcacaatc tggcggtcaa agcgcagaaa atgattctta tagaacccct tcaccacttt
     7141 ctcgcagtaa taattaaacc gctcgcgcag cattccggca ttggtgtgct tatcggcctg
     7201 cgccagtttg gactcgtccc aggcatcgac atccggccac gggaagaatt gcagcgcggg
     7261 cgcacctgcc atatctcccg gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg
     7321 ctgtttacag tggtggagat aatcggtcca cacggcggca atgtctgcca gacggttttc
     7381 gtcggcaggc gctagcgggt caagtccttc gcacatcatt cgccatttcg ccgaccattc
     7441 gccgcgctga ccattgagta agcccgtcat ctggcgcgac cagcttaagt aatcctgcgc
     7501 cagcatcggc aggtcgagca accattcgcc gggataatcc acaatttcca gatacagcgt
     7561 ggaggtatcc ttaaagtggc gcagcagcgc atcgttcgat ttaaaacgta acgccaggcg
     7621 aatttcactg acgccgcgcg taggcgtcgg ccaggcggga ggatcaccat ataactgcgc
     7681 cagtccttca tcataggtga agcgcggaat gccaaagtca cgctggggaa tgcgttttac
     7741 gcccagcaag cgctcttcac gcaccgcgct taacagcggc aaacgtgctc cggcatgaat
     7801 attgagcaac tgattaacca tcgcggtgat aaacgctgtt ttgccgctgc ggctaagtcc
     7861 ggttacagcg aggcgcagat gtctgtcgac accccgattc accagcgcat tgagttcatt
     7921 tttaagtcgc ttcattgctg tcctgtgctg ctctgaagac cagaaaaaaa atcattcgta
     7981 aggatatgaa tgtgggggcc aggccattga ttatcaactg cccgtgttgt catcgacggg
     8041 cgaactccat aagatgtgca gcattcacgt tcggacggag ttcctgacct atgtccccta
     8101 ctatttatga tattgccagg gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga
     8161 ataagcaaac taatatctcc ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat
     8221 tacagtatca gccaaacaag ctggcccgcg cgctgacctc ttcgggtttt gatgccatta
     8281 tggtgatttc tacccgttcg accaaaacta cggcgggtaa tccgtttttc tctgaagttt
     8341 tgcatgccat caccgccaaa gcggaagaag aaggtttcga cgtgatatta caaacgtcgc
     8401 acaatccggc agaagatctg caaaaatgcg aaagcaaaat taaacagaaa atgattaaag
     8461 gcattattat gctcagttcg ccagcggatg agtcattttt tgcccaactc gataaatacg
     8521 atattccggt ggtggtaatt ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg
     8581 ataccgataa ttttggtgac agcattgcgt tgaccgatgc attaattgaa agtgggcatc
     8641 aaaatattgc ctgcctgcat gctccgcttg atgtccatgt ttcggttgat cgcgttaatg
     8701 gctataagca aagtctgtcc gcgcataata ttgcagtgcg tgataaatgg attgttgacg
     8761 gcggttatac ccatgaaaca gcactgcaag cagcacggca attattaagt cagtcaccgt
     8821 tgcctgaggc agtgtttgcc actgacagcc tgaaattaat gagcatttat cgtgcggcgg
     8881 cagagaaaaa tattgctatt ccgcagcagt tagcggtggt gggttatagt aatgaaacgc
     8941 tgtcgtttat tttaacgcct gcaccaggcg gcatcgatgt tccgacgcag gagttaggtc
     9001 aacaaagctg cgagttatta ttccgcttaa tttcgggaaa accgtcacca cagaatatta
     9061 ccgttgccac gcatatgacg ctgaaataaa taccagctac cttgcctgaa tgccggatgc
     9121 gatgctggcg catcttatcc ggcctacatt tatagatcgg ataaagtact cgcgccgcat
     9181 ccggcaattt agcccattat cagaacgagt aatttacgcc gacacctgca taatggaatt
     9241 tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc gtattccagc gaaacggaaa
     9301 gtccgttctg gaaatcataa ccgtaaaata aacccacacg gttaaaatca tggccttcac
     9361 gttcaatatc atcctgccag tcccagttac tctagcgatc cagcccaatg cgcgtatacg
     9421 gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc gcgaatttct tgtgtggaaa
     9481 actcaccgtt attgcggctg tcgtccatat tgaagccgcg ctcgagataa tagttcactc
     9541 gcaaagcaac cgtttcgttg aaggtatatt gcagacctgt ttccgtttcg acacgggtat
     9601 cagcgtaacc ggtcgtgttc aaatcgttgg caaatttata catcgacaac caaccgttga
     9661 aacgtaaatc atcagtcagt ttcacatccc agtctggcgc gattttccag cgctgcatat
     9721 tcgccgtgtc tttgcccggt tcatcaacgt agtgataacc ataattacgg aaaccgccgg
     9781 tcaggccgaa actgaaatca tcgctttcga ggaactgata atgcacctcc agctccgggc
     9841 gatcaaacct cgttccacgt ttacccgcac tataatctac cggcccttcc tgataatagg
     9901 ccagagcaat tctccacggc ccgttggcgg cattaaaata gactgaaggc tccgccagcc
     9961 catccatatc ttcgccataa ccctcgacgt tttctatttc gtacatcgca ccgatattaa
    10021 agtgccagtc gctcttttcc tcggcctgtg cgcaggccat tcccgcacac atcaccagtg
    10081 cggtacaggg taataacttt ttttgttcct tgtattatcc tgggggaatg ccttccccct
    10141 gtatttagcg aattgctatt tccgtttctg catcaaagaa atgacatttc gtcatatcaa
    10201 aatgaatagt gatattttct cctgcatgat aatcatttaa cgcaccagca cggaccacta
    10261 actcgtgccc ccaaccgtgg tgtagagcat aaattccgca ccggttaatt ctgccacgct
    10321 aattttggcg gaaatgttat tttcctcttg cgcgtccgga tgaatatctt ccgggcgtat
    10381 tcccatcacg atgggcttat gcaaactttc ctgtgttttc agaaccgcta atttctcttc
    10441 gagaatggtt aatttaagcg tttccgtaac gaatttatcg ccatcgatcg tgccgcgaat
    10501 aaaattcatc gctggtgatc caataaatcc ggcaacaaac atattcgcgg gttggttata
    10561 aacggttttc ggcgcaccta cttgctgaac aatcccgtct ttcataatca caatccgcgc
    10621 cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tgttcagttt
    10681 ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc agcttggcat caaggttaga
    10741 gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac
    10801 acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc
    10861 gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat
    10921 cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg
    10981 gaacaccatc gcgatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat
    11041 caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgattt
    11101 accgcagccc gacgggccga caaatacgat gaactcttta tcggcaattt ccaggttgaa
    11161 gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg
    11221 agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca
    11281 gggagtccgt tgagggcaac agtaattgcg ccccggttaa gcccgcaccg atccccaccg
    11341 agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac
    11401 atgcctgcgg cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt
    11461 tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagcg
    11521 ccgctaaaat cgtcggcgca ttcagggaga cggaagccag ccctaccgga atctgctgtg
    11581 cacggagctc tgccagcaaa ttgcgaatac cgggcagcac agcattaacc gtcagctcgc
    11641 gcagtgagtg gacgtaaagc agatttttgc gatacgccag ttgcgcccgc tcctgcgggt
    11701 taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga
    11761 tcccttttag agattcgtta aactgcgcat caatactgat gccaatttca gcggcaatct
    11821 gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa
    11881 ttaccccttg cagtttcatt catcctcctg atgcgtggta gcggtcccat tgaagggcaa
    11941 aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct gaccgtccaa
    12001 acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcga gagtgacctg
    12061 taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct caagtaacgc
    12121 cggatttaga tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc
    12181 cagccagata gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc
    12241 accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca
    12301 gcgtgcagca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg
    12361 ttcataaaat tgcagattgg caagacacga tgccgatgag aactgctctg gcagcatgta
    12421 attgagcatc accacatcag cttgtttgag gatctgcatc tcgttcactt ctgcgcgtga
    12481 ataatccagc agaatggttt gcttccccgc cgccgctttg tatttcgcca gattaatcgc
    12541 cggcttagtc ataaacgaat catcctgcgg caaaacaccg tcgggctgaa tttctggcag
    12601 ccgcagttct ttaaggaaca tttcggcacg atgaataaac gcgtcgtcac tacagccaaa
    12661 ctggcgggca atactcagcg cctgctggac gttgtaatac accatatagc tggtgaaggc
    12721 gttattattg acatgttcgg tatattcgtc tggcccaata acatcatgaa tttccagacg
    12781 gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat
    12841 gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca
    12901 ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc
    12961 ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc
    13021 ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa
    13081 gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatggaa
    13141 cggcagcaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa
    13201 gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc gtcatgatgc gcagatgata
    13261 aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg ccatttaccg tgatacggcg
    13321 tttctgccac cattgacgcc agttttctgt tgatgctgca agatgttggt cgtaactctg
    13381 ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc cactcgtcta aagcagcttg
    13441 cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg
    13501 aagctgcgta ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac
    13561 atcaccgctc acctggcagc aacaactgat ggccacatca ctgcgcccat cctgggtggt
    13621 gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg
    13681 gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc
    13741 gtccagtggc ataatagtga tttccagcgc aatgagcggc agttggtctg ccgaaacaaa
    13801 gcgacggctg gcgatggtgt aacctgcgcc gttgctggta cgccagacaa cgctacggcg
    13861 taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac
    13921 ctcaccattt atggcaatct ccatccccaa gatatcaggc agattcacca gttcgttgat
    13981 ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt
    14041 gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga
    14101 tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt
    14161 cataattcca cacgcgtccc tgtttctgcg gattgatata acgccgccac cagttgctgg
    14221 atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac gtgattgata
    14281 aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca tttcccgttg catcagcgtc
    14341 attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accggcttta
    14401 tcaccacaaa agctgacgtt cataatcaac tgttcgcgga tgttgagtgc aaatgacgtt
    14461 tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg
    14521 ctgtaagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt gccgatcttt
    14581 tgaaaactat gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca
    14641 tccagcatat gaatgccgat gtcgatcagc aggccaccac cctgcagttc tttattggta
    14701 aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc
    14761 tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaacgat
    14821 ggtgaaagtc gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccacg
    14881 cctgttctgt cgtcatggcg ggcggttttt cgcacatcac atggcagcct gcttccagtg
    14941 ccatcagggt atgttcgtaa tgaaaacggt taggtgagcc gacgctaacc acatcaggtt
    15001 tcaccgccag cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg
    15061 ccagcgcctg cgcctgggaa aggtggctgt cacagacagc caccagttcc agatcgttgc
    15121 gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcccgcgcct attatcgcga
    15181 cccgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc
    15241 aggccaacga atcacggtac gcctgggcag gatcttctgc ccggatacgc ccttcataca
    15301 ccacataacc ctgatagtta tcggcgcgca gctgttcaaa cagcgcgtgg aaatcgaggg
    15361 tgccactgcc cggctggtaa cgatggttat cggcaatatg cacatgaccg agcaagtcac
    15421 ggttgtcgtg cagcgcctgc gccaggttat cttcttcgat gttcatgtga tagaaatcgc
    15481 cgataatctg cacatgttta agattgtttt cgacgatgta acgacgagca tcggcgaggg
    15541 tgttgatcat atgatcctga tagcggttta acggttcgag atacaccacg gttccggtac
    15601 gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cagtttgcgg tcgccgtcca
    15661 ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa
    15721 cccccccact tctgccagcg cctcgagaat gcgttcgatc tgctttaagc cattaagacg
    15781 acgctcttcg ataaagtcac caatccagcc gtcatagccg ccgcaggcgg tggtcactgg
    15841 taaaccggtt tctttgatcg ccgctttgac ttcttcgatg ttgttgacca gcagtttgcc
    15901 gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatctct cgcggatatt
    15961 ttccggaaag aatgcctgat tctgtgtgcc gattttcatt ttttcattcc tttgaattaa
    16021 aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg catgtagctt
    16081 tccgggctgt tggcaaaggt cactaccggg tcgattaaat cttcgcaatt gagataaccg
    16141 ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg cggataatcc
    16201 gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa atgcgcttcg
    16261 cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacatagga gatggtgccg
    16321 ccgtaggcca ggccgcgcag cgccgattgc agcgcgtcgg cgtaaccgct ggtttcgata
    16381 atcacatcag ccccctgctt gccggtcagc gttttgatct ctttgcctac gtcagtgcca
    16441 atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg atgggcgata
    16501 ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg ccagttggat ggcaatttga
    16561 ccgatcgcgc caagccctac caccacgaca aaatccccta cgcgtacgtt ggcatcgcgc
    16621 acgccgctca tggcaaactg cgccgggtcg taacagacgg cgtttttcca ggagctgcct
    16681 tgcggcattt tgcgcagctt gtagttattc actgcgttaa tgatgaccgt ctcggagagc
    16741 gggccgtagc cgcatacgct gtcgcccacc gcgtagtctg taacgtcgct gccgcactcg
    16801 ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat gccgcgcggc
    16861 gcatctgccg gacgcggggt gaacatctgc cactcgccgt taaagtcttc atcgataaac
    16921 gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg
    16981 cggatcttca cttcattggc taaaatcgcc cgatcttcat actcaaccag cgcagcaaca
    17041 cgcggtgctg tggctactaa ctttttcatg taaaactcct tgtaaagaaa ctggccgctt
    17101 tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccaa aagcgcatac
    17161 ataatcacca ccggcaatgc ggtcaccagc gaggccgcca tcatccgtcc ccagatgtaa
    17221 tcaggcgtac tgaacagcgc gttcaggccc actggtaagg tgaaattgct ggcgctggag
    17281 aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta gacgaacacc
    17341 gatatcagcc cggacatcgc caggggcacg gtgatacgga agatgatttg cagacggttg
    17401 aggccatcca tcatcgccgc ttcttcgatc tcatcaggga tggtgtcgaa gtaacttttc
    17461 agcataaaca cggcggtggg cagggtttgc gtcaccatgg taatgatcag cgccatctcg
    17521 gtgtcgtaaa taccaagcgc ggtgatgatt ttgaacagag gcaccaccag caaaatgcca
    17581 gagaacatat acaccgtgta aaagctggcg ttgatggtca tccggccttt aaagcgcagg
    17641 cgggaaagcg cgtaagctcc gagaatgccg agaaataccg ccaccacgga ggaaaccacc
    17701 gacaccacca tgctgttacg gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg
    17761 tcgacgtaat gctccagcgt ccactgttgc ggcagcagcg taggatgcag cgatatcgcc
    17821 tctttcgcgc ttttgaacga ggtcatcagc atcacaaaaa acggaaacag cgtgatgatg
    17881 agaaacagcg ccagcccgca gtaaaaaccg atacgactga gcgtgcgttt atttgttgcc
    17941 attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga tgaagagcac
    18001 caccgagatc gccgccgctt tccctaagtc attaaaggca aaggcggttt tgtagagata
    18061 gacaccaaga atatcgacct tggttgtcag cagataaaca tcagcgaaca tgtagaacat
    18121 ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataattgcgg gcagcgtgac
    18181 gatgcgaaac cgctgccagg cattagcgcc atccatctct gccgcttcgt acagcgattt
    18241 gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt agcgccagat
    18301 ggcgaacagc accaccagca caaagctact gcccggatta tcaaaccaca gcggtgcctg
    18361 ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc cgttgttgaa
    18421 catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag
    18481 cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa aacatcgcca cggccagccc
    18541 gagcacggtg ctgcccgcca ccaccagcgc cgtataccag accgtcatcc acagcgagtg
    18601 ccagaagccg ggatcggaga gaatgcgcac atagttactc accccaacaa acgttgactc
    18661 gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca ccatcggcca
    18721 cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcagcagcg caaacggcat
    18781 atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt ttagtgctgt
    18841 tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag atcggcttta
    18901 cccacggtga cgttatgcac catggagctc accacaccag aacccgtcac atcgcccatg
    18961 cgggtaaagt ttttatcccc tactgcgcca aagacctgaa tatttggcag ttcaccgatt
    19021 agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca ggtggcggta
    19081 gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac ccagtcggca
    19141 atgttgtccg cctgctccat aaaggtgaca aatttttctg ctgcttcggt cttttgtccg
    19201 gcggtaatgg tcagcgaggt caacatgccg tagaccgcag agtttttctc ggttggcacc
    19261 acgaaaccga cgtttttcgg gtcgccttct ttaatcacag ccggaaggat ataggtggag
    19321 taaatcgcca tcggcgcggt gccgttcata aaggcgtctt tcacttccat gatgtcgtta
    19381 gaacccggca tggtgttggc agcaaggtcg cggtaatagg tcagcgcctg catcatctct
    19441 ggtgtatcaa gggtgatttt gccttcggcg ttaaagacgt tggcctagtt ggataacgca
    19501 aactgggaga aggattgttc cgtcagcacg ctttctgccg taggcagcgc aataccgtat
    19561 tttttattcg ccgggtcatt cagtttctgt gcaacgtcca gcagctgttg ccagtttttc
    19621 ggctcctcca gccccgcttt tgccagcaca tctttgcgat accagatgcc gccaatccag
    19681 gcgctgacag gaacaccggt ccatgcgcta ccatcttcgg tacgcacaat acgcagtacg
    19741 ccatcgtaaa acgcgccttc accaacgttg ctgatgaccg tggcaaccgc tttgcgatcg
    19801 ataagctgct ctttatccat aactttggcg tagtcatggc tggtttcgat cacttccggc
    19861 agcgagccgc tacgtgaaag ggtgatgact ttggtgttat gagcgtcttc ttccacgggc
    19921 acttgtttaa cggtgatacc tgggttttct ttttcaaaac gggcaatcaa tttgctgata
    19981 actgcctgac gctcctgctc caccgaagaa tgcataaatt cgatggatac atgggtttta
    20041 ttttcttctt tacagcctga aatcagggcg caggaaacca gtgctgataa cagcacgatt
    20101 tttgatttaa tcatgagatg ttccttttta tttaatccac ataacctgcc aggggcgcag
    20161 cgttatttca ttacccaata tatcaacttc a
//
LOCUS       DAJTRW010000073        20084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRW010000073 DAJTRW010000000
VERSION     DAJTRW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO326_002804"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO326_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5967131.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO326_002805"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO326_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5967132.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO326_002806"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO326_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO326_002807"
     CDS             1631..2341
                     /locus_tag="OO326_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5967133.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO326_002808"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO326_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5967134.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO326_002809"
     CDS             complement(3469..4119)
                     /locus_tag="OO326_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5967135.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="OO326_002810"
     CDS             4282..6480
                     /locus_tag="OO326_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5967136.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="OO326_002811"
     CDS             complement(6449..6889)
                     /locus_tag="OO326_002811"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5967137.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO326_002812"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO326_002812"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5967138.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="OO326_002813"
     CDS             7607..8305
                     /locus_tag="OO326_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5967139.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="OO326_002814"
     CDS             8534..9742
                     /locus_tag="OO326_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967140.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="OO326_002815"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="OO326_002815"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5967141.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="OO326_002816"
     tRNA            11849..11925
                     /locus_tag="OO326_002816"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="OO326_002817"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="OO326_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5967142.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="OO326_002818"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="OO326_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5967143.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="OO326_002819"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="OO326_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5967144.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="OO326_002820"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="OO326_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5967145.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="OO326_002821"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="OO326_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5967146.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="OO326_002822"
     CDS             complement(18540..19811)
                     /locus_tag="OO326_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5967147.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4812 a         4974 c         5275 g         5023 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DAJTRW010000074        19593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRW010000074 DAJTRW010000000
VERSION     DAJTRW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1411)
                     /locus_tag="OO326_002823"
     CDS             complement(<1..1411)
                     /locus_tag="OO326_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation and assembly module TamB"
                     /protein_id="HCR5967148.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLI"
     gene            complement(1408..3141)
                     /gene="tamA"
                     /locus_tag="OO326_002824"
     CDS             complement(1408..3141)
                     /gene="tamA"
                     /locus_tag="OO326_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5967149.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            3347..3985
                     /gene="msrA"
                     /locus_tag="OO326_002825"
     CDS             3347..3985
                     /gene="msrA"
                     /locus_tag="OO326_002825"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5967150.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4308..5651
                     /locus_tag="OO326_002826"
     CDS             4308..5651
                     /locus_tag="OO326_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5967151.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(5713..5919)
                     /locus_tag="OO326_002827"
     CDS             complement(5713..5919)
                     /locus_tag="OO326_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5967152.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            6244..6798
                     /locus_tag="OO326_002828"
     CDS             6244..6798
                     /locus_tag="OO326_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5967153.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(6861..7799)
                     /locus_tag="OO326_002829"
     CDS             complement(6861..7799)
                     /locus_tag="OO326_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967154.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(8011..8751)
                     /gene="cysQ"
                     /locus_tag="OO326_002830"
     CDS             complement(8011..8751)
                     /gene="cysQ"
                     /locus_tag="OO326_002830"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5967155.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8941..10884
                     /gene="cpdB"
                     /locus_tag="OO326_002831"
     CDS             8941..10884
                     /gene="cpdB"
                     /locus_tag="OO326_002831"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5967156.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(11010..11390)
                     /locus_tag="OO326_002832"
     CDS             complement(11010..11390)
                     /locus_tag="OO326_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5967157.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            11479..12339
                     /gene="qorB"
                     /locus_tag="OO326_002833"
     CDS             11479..12339
                     /gene="qorB"
                     /locus_tag="OO326_002833"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5967158.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            12448..13413
                     /locus_tag="OO326_002834"
     CDS             12448..13413
                     /locus_tag="OO326_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5967159.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            13521..14183
                     /gene="ytfE"
                     /locus_tag="OO326_002835"
     CDS             13521..14183
                     /gene="ytfE"
                     /locus_tag="OO326_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5967160.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(14228..15640)
                     /gene="cycA"
                     /locus_tag="OO326_002836"
                     /gene_synonym="dagA"
     CDS             complement(14228..15640)
                     /gene="cycA"
                     /locus_tag="OO326_002836"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5967161.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(15948..16568)
                     /gene="fklB"
                     /locus_tag="OO326_002837"
     CDS             complement(15948..16568)
                     /gene="fklB"
                     /locus_tag="OO326_002837"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5967162.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            16787..17332
                     /locus_tag="OO326_002838"
     CDS             16787..17332
                     /locus_tag="OO326_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5967163.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(17316..17963)
                     /locus_tag="OO326_002839"
     CDS             complement(17316..17963)
                     /locus_tag="OO326_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5967164.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            18175..18966
                     /locus_tag="OO326_002840"
     CDS             18175..18966
                     /locus_tag="OO326_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5967165.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            18976..>19593
                     /locus_tag="OO326_002841"
     CDS             18976..>19593
                     /locus_tag="OO326_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5967166.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRI"
BASE COUNT         4896 a         5078 c         4781 g         4838 t
ORIGIN      
        1 taatcaagcc attgagtttc gacggccact ccgggaactc tttggcggtg ttaatgccat
       61 taagcgttag ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt
      121 cagttttccc ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac
      181 ctttggcatc aagggtaatg gtggctggcg ggatctcctg tcccttcact gccgtacgca
      241 tagagagcgt gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat
      301 actgcttctc accagtgaac ggccagtaaa gctgtttgct gttcacttcc acgttgagtg
      361 gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa
      421 ggttaacgcc tatctccagc tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt
      481 cacctttcaa cggctccacg ttcagtgtgc tgttgagggt gatatccacc ggccagttgt
      541 ctgacagctg cgccgtgccg ctggcgttga cgatcccctg gttggaatcg atatccaggg
      601 cgtccagttt agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga
      661 tgtccgtgtc gcccgtcacg cgcagctgct cgcccttaaa ctcttcaatg ttcaggttaa
      721 gcggcaaatg cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg
      781 tttcgccgag cggcttttcc tccggctgcg gattttcaat tttcggttcg accacttctt
      841 cctgcgccac ttccaccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt
      901 tcagggtcag ggttttctcc tgccagttca ggccggaggt gaagtccatc accgacaccg
      961 tggtgtcatc aatcttgatg ttgacgttgt ccagcgccac ccgtgtcagg gtgatgggat
     1021 acggcgtgga gagatccagc ggaccgctat cttcctcttc ttcaacctgt tcagaaggag
     1081 gcattttttt actgtcgatg ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa
     1141 cgctactgtt ccacaggcac tcaagaccga cccccagatg cagattaccc gcttttaccg
     1201 ccacgcctag ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga
     1261 ccttgccaat atccagtcct ggcacccagc gatccgccgc tttaaataca agatgcaggc
     1321 cgctggtggt gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca
     1381 cgccgaggct gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg
     1441 taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac
     1501 cggcgattcc cagcgtacgc cgaccccggt accggtttta aagtcgctgc ggcgaatatc
     1561 gcttaccgct tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta
     1621 ctggtattcc agcgatccgg ttatcaactt cgaggcccct ttcaggtcac cgttggcgta
     1681 tttcggggcg atagatttgt atttgtagcc gcgaatactg cgatcgcccc cgacgaagaa
     1741 acgcagatcc ggcggtactt tgtcgaaatc accggtttca atccagccca gcgtgccgcg
     1801 tgtcacaaag cgatggcgat cgtacagtgt gcggatccag acgtgctgcg cctggaaaac
     1861 ggagaaatcg acatctgaac cccaggccgt gttggagtag tcgatagagt agcgttgcga
     1921 gtctccccag gttggcatca ggccaccacg agaacgcgtg cggctaatca tcaccccagg
     1981 ataaaacagc atcgtggtgt tggtaatttc accctgggta aagtggtcga ggctccagcg
     2041 caggttaatg gcacgctgcc agccgctgga gagatcccag tagcgagaag ccaccagcgt
     2101 agtggagtca gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata
     2161 atattgttcc agtggattct tcagcagcgg cattttatag ctgaagtcga gggtctgttc
     2221 cggcgcggaa atactggtac tggtggtcaa actgtgaccg tatgagttca tccacggctt
     2281 tttccacgtc gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat
     2341 ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc
     2401 tttatcaaat tgtggagcca ccaccaccga gttaaaccag ccggtagcag aaagtcggcg
     2461 gttcagttcc gccagatctt tcgattcata ctcatcgccc tctttaaacg gcaccagatt
     2521 ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg
     2581 ttcgccactg ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag
     2641 ctgcgctttg gtaaattcgc tatcgaaata acctttacgc aacgcaatac tggttaagga
     2701 ctttttgaaa ttttcataat cgccctggtt cagtaccgta ccaatagccg ggcgagtatc
     2761 gagcaatttc aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc
     2821 gccaattaac accggcacac ctggcgtgac tttggcgatc aatacctgcc gccctttctt
     2881 tggcggtgga cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc
     2941 ttcacggatg gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact
     3001 ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc tgtcccgata acccctcgac
     3061 ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca
     3121 taactgtcgg atatagcgca cattttctcc tgaatatcct tttttcctgc ccctggaacg
     3181 ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa
     3241 tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct
     3301 tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga
     3361 taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt
     3421 agccacgctg catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat
     3481 tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg
     3541 cgtttacagc accgccgcag gctataccgg cggctatacg ccaaatccga cttatcggga
     3601 agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt
     3661 catcagctat gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat
     3721 gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga
     3781 acaggatgcc gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga
     3841 tgacgatcgt cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga
     3901 tgaccaccag caatatctgc ataaaaaccc atatggttat tgtggaattg gcggaattgg
     3961 cgtctgtctg ccgccggaag catagcgtta cgggtacaaa tgtagtttgt tgataaagtg
     4021 ctctttgtct atgcctgatg cggcttaaac gacttaccag gcctacaaaa tcgcccaaat
     4081 tcaatatatt gcaaggactg cgtaggcctg atcggcatag cgcatcaggc agtattgcat
     4141 ttatcagcag tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc
     4201 cactgaaaat gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt
     4261 aatcaacttt cccttccgag ggtctggcct gaaaggtcgg ataagatatg ttaaacagta
     4321 ttttagtcat actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc
     4381 ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc
     4441 aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc
     4501 tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc
     4561 acagcctgtt ctcccgctat atgtcggcag agctctctga gcaactgagc tttattctct
     4621 ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg
     4681 gtatgattgc gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt
     4741 acgtttgcac cccgctggtg tggttcttca acggcctggc gaacatgatc ttccgtattt
     4801 tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg
     4861 aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct
     4921 ttgagctgga atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt
     4981 ttgatctcca cgaagatgag caaagcctga agaataaggt ggcggaacat ccgcactcta
     5041 agttcctcgt ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat tctaaagacc
     5101 tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca
     5161 acacgctgat tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg
     5221 caggtgaaga cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc
     5281 tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg tcaggggctg gaagagcaga
     5341 ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg caccccaatt gacgacgtca
     5401 tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct
     5461 ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct
     5521 acaaatttga agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgga
     5581 tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa
     5641 gcgtcgcgta acccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
     5701 atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga
     5761 catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa
     5821 ttcaaaagcg ccaatgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt
     5881 cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt
     5941 acgcctgtgc ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt
     6001 gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac
     6061 aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca
     6121 tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc
     6181 acatatgatt aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac
     6241 accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc
     6301 gcacatcagt tcgaaatcgg tcagcgagtg ccgccgattg gcattaccga tcggggcgag
     6361 ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga
     6421 aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg
     6481 ctgattgaag cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccaccatt
     6541 gttaacaccg acgacgcgat tccgggttcc ggcatgtttg tgcgcagcag tctggagagt
     6601 aataaaaagc tttatccctg gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt
     6661 gcctggcagc tggatgaaga aagttccgct gtagtggtgc tggataaaga cggtcgcgtg
     6721 caatgggcca aagacggggc gctcacaccg gaagaggtgc agcaagtgat ggacctgctg
     6781 caaaagttgc tcaaataatg gcaataacat aaattctgga aaaataattt tcccctatcg
     6841 ttatctatgg gataggggaa ttaagtctct gagttcttac accgaactaa tactggtaac
     6901 gctagttgct cttttttaaa cgcctcaatg gtcgatttat atttctcgca aatcttttct
     6961 ttggtaatat tactatctgc ggccagattc gcacttacta tcgcatccac ttgttcggct
     7021 aattcgactt gttggcaacc tgcattaacc agaaaattat aaataagaga gtaatcttca
     7081 tcctccaaaa caacagagga accattacga aaacgttcat cccctccata gattttaagc
     7141 gcaaagccca aagattctat gactatggca aaattatcat caattatttt ttcgtaaata
     7201 taccatccat cgcattcttt aattccaccg cctggtattt ggagttcaaa tttaccccgt
     7261 tttaatttgc ttactacatc ctccaataag gcataaccga aagatacgtc accaaacgcc
     7321 tggcgaatat aacctttaat cctttttggt atgtcagact gttttttctg caaaatatca
     7381 caggcataca atcttctgtt ttgttctaac ggaaactcgc ggtttttgaa atccatgaat
     7441 gagttatact catttattgt tatgggaatt tcttcgtatt caaacccact gcctcgcaat
     7501 gttattgtat attcagcacc gttatcttta aggctgaaac tgataagaaa attatccatg
     7561 gcattaacag tttcattttt tgatgaaagc acttgataac tatttttatc aatctgtgtt
     7621 gcgcaacgaa aggactcccg caaagttaac agactatcat aattcgaaac ccaatctttt
     7681 ttcttaaatg catgctgaat attattaaaa aaatcgtttg aatgtgtttt tgtatgcgat
     7741 gactttacaa ataactccac cgcaccaata aaattattgt gtctgatcct gggtaacata
     7801 tggctacctt attttatttg gttattttat gtcgccatta aatatctcat tatagaagta
     7861 aactatcaaa aaacgctcat tattaaaatg taacttacta gcaattgaat ttttgcactt
     7921 taaatacaat ctctttttat ttataacttt ttaggttggg ataaatcagg atggcagaat
     7981 caggaaatac actgtttctg ccatctgaat ttagtaaatg gacactctga accccggatt
     8041 caggaacgac tcgcgcggag tgtaatccag cggtttaccc tgccagtcgt gaacgtgcgc
     8101 tccggcagct gcagccacag catgtccggc ggcggtgtcc caaatattcg ttggtccgaa
     8161 gcgcgggtac agctgcgcct gtccttccgc caccaggcag aatttcagcg aagagccgat
     8221 ggacgtggtc tgatgttcgc caagctgttg cagatactct ttcagctccg catctgcatg
     8281 ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg acctgaatca gcttgcgcac
     8341 accgcactct tctttccagg ccttgccttc tgccgcgctg tacattacgt tcattaccgg
     8401 cgcatacacc acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa
     8461 ttcgccatta cgtttaataa actctttagt accatccagc gggtctacca gccagtaacg
     8521 ctgccagtgc tgacggactt cccaaccggg aggatcttct tcagaaagga ccggaatatc
     8581 cggtgccagc gtacgtaaac cgtccatgat aacggtgtga gcggcaatat ccgctgccgt
     8641 taccggagaa ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg
     8701 cataatggca tcgcctgcat tccgtgcaag ctggcatact tgatctaaca tttctccacc
     8761 tcgtctctgt gagcggtgtt aacttattgt tttacttata ccctatcgtt aatgaatgcg
     8821 ccaactgtga tagtgtcatc attttcaaag cgtaaaattg tggcattctt cactgttcta
     8881 taagtaaggc gtttattctc ccttttcttt cgtattcccg atgataaaag gatgtccctg
     8941 atgattaagt ttagcgcaac gctcctggcc acgctgattg ccgccagtgt gaatgcagcg
     9001 acggtcgatc tgcgtatcat ggaaaccact gatctgcata gcaacatgat ggatttcgat
     9061 tattacaaag acaccgccac ggaaaaattc ggactggtac gtacggcaag cctgattaac
     9121 gatgcccgca atgaagtgaa aaacagcgta ctggtcgata acggcgattt gattcagggg
     9181 agtccgctgg ccgattacat gtcggcgaaa ggattaaaag caggtgatgt tcatccggtt
     9241 tataaggcgc tgaatacgct ggattatacg gtcggtacac tcggcaatca tgaatttaac
     9301 tacggtctgg attacctgaa aaatgcgctg gcgggagcga aattccctta tgtaaatgcc
     9361 aacgtcattg acgccagaac caaacagcca atgtttacac cgtatttaat taaagatacc
     9421 gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg
     9481 ccgccgcaaa tcatgggctg ggataaagct aatttatccg gaaaagtgac ggtgaatgat
     9541 attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcatt
     9601 gtcgttctgg cgcattccgg gctgtctgcc gatccgtata aagtaatggc ggaaaactca
     9661 gtttattacc tcagtgaaat tccgggcgtt aacgccatta tgtttggcca tgctcacgcc
     9721 gttttcccgg gtaaagattt tgctgatatc gaaggggctg atatcaccaa aggcacgctg
     9781 aatggtgttc cggcggtaat gccgggcatg tggggcgatc atcttggggt ggtcgactta
     9841 caactcagta atgacagcgg taaatggcag gtgacgcagg cgaaagcgga agcacggccg
     9901 atttacgaca tcgccaataa aaaatccctc gcggcggaag acagcaagct ggtagaaaca
     9961 ctcaaagccg atcacgatgc cacacgccag ttcgtcagca agccaatcgg taaatctgcc
    10021 gacaatatgt atagctatct ggcgctggtg caggacgatc cgaccgtgca agtcgtgaac
    10081 aacgcgcaaa aagcgtatgt cgaacattac attcagggcg atccggatct ggcaaaactg
    10141 ccggtgcttt cagctgccgc accgtttaaa gttggtggtc gcaaaaatga cccggcaagc
    10201 tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct ttatctctac
    10261 cccaatacgc tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc
    10321 tccgccggac agtttaacca gattgctcct aacagcacga aaccacagtc actcatcaac
    10381 tgggatggtt tccgcactta taactttgat gttattgatg gtgtgaatta tcagattgat
    10441 gttacccaac ccgcccgcta tgacggcgag tgccagatga ttaatgccaa tgcagagcgg
    10501 attaagaacc tgacctttaa tggcaagccg attgatccga acgctatgtt cctggttgcc
    10561 accaataact atcgcgctta cggcggcaaa tttgccggta cgggcgacag ccatatcgct
    10621 tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa
    10681 cgtgcggggg aaattcatcc ggcggcagat aacaactggc gtttagcacc gatagctgcc
    10741 gataagaaac tggatatccg tttcgaaact tccccgtcag ataaagccgc agcgtttatt
    10801 aaagagaaag ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt
    10861 tatcaggtgg atttgagtaa gtaaaacact tctttttcgg cctataaatc aaccgcatcc
    10921 ggcatttatt ggcggatgcg atgctgccgt atcttatcca ccctacaagc catgaaccgt
    10981 agaccggata agcgcagcgc atccggcagt tatgccgcac gttcatcccg caccgccaac
    11041 acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc gctcacctgc
    11101 tcgcccagcg gcgtgaggct atattccaca tgcggcggca ccaccggata cgcgatacgg
    11161 ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt ttcactcacc
    11221 ccaccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag cgccaccaga
    11281 atcaacaccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg gcactgttcc
    11341 gcaaagagat taccctcttt cagttgttgt gacaggctaa cctgactcat ttcatactta
    11401 cctttttgca cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa acacaagaca
    11461 aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact tggtcactat
    11521 attattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat cgttcgtaat
    11581 ccggcaaaag cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc tgactacagc
    11641 gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct gatctcttcc
    11701 agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc aaaggcggct
    11761 ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc gctcggcctc
    11821 gccgatgagc acatcgagac ggagaaaatg ctggctgatt ctggcatcgt ttacaccctg
    11881 ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg ccccggcagc actggaacac
    11941 ggcgtattta tcggtgcggc tggcgatggc aaaatcgcct cagcaacgcg ggcagattat
    12001 gcgacagctg cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt ttacgaactg
    12061 gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa acagagcggc
    12121 aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact gaaaagcgtc
    12181 ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc atcgaaaggt
    12241 ggtctgtttg ataacagcaa aacgcttagc aaattgattg gctgcccaac gacaacgtta
    12301 gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt ggcatcctcc
    12361 cgcatcctct ctgataatga cgggatgccg ggagcaatca tgtctgcttc ctgaacgttt
    12421 cttctgacag accaatggat gccagtaatg attagcggcg tgctgtacgc cctgttagca
    12481 gggttgatgt gggggcttat ttttgtcggg ccgttgatcg tgccggaata cccggcgatg
    12541 ttgcagtcga tggggcgtta tctggcgtta gggttaattg cgctacccat tgcctggctg
    12601 ggacgcgtgc gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt gatgctcact
    12661 atgatgggca acctcattta ttacttctgc cttgccagtg ccattcaacg tactggcgcg
    12721 cctgtttcca cgatgattat cggcaccctg ccggtggtga ttcctgtctt tgccaatctg
    12781 ctttatagcc agcgcgacgg caaactcgcg tggggaaaac tcgccccggc actgatttgt
    12841 attggcatcg gcctggcgag tgtgaatatt gctgagttaa accacggact ccccgatttt
    12901 gactgggcac gttatacctc tggcatcgtg ctagcgttag tttccgtggt ctgctgggca
    12961 tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca tccgatgatg
    13021 tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta tctcgtcgcc
    13081 tgttactggc tgaatataca aacgccggac ttctccttac cttttggccc ccgtccgctg
    13141 gtgtttatta gtctgatggt tgcgatagcc gtgctttgct catgggttgg cgcactctgc
    13201 tggaacgtcg ccagccagcg attaccgaca gtgattctcg ggccgctgat tgttttcgaa
    13261 acgctggcag gtttgctgta caccttttta ctccgccagc aaatgccgcc gctaatgacg
    13321 ctgagcggta tcgcgctgtt agtgattggc gtggtcatcg cggtcagagc aaaaccagaa
    13381 aaacctttaa ctgaatctgt ctcagaaagt tgacatgctg gcagtgagtt aaataagcct
    13441 ctgctacgta agggttatag cttttgcctt aaaggtgtat ttaaaataca tcttatctta
    13501 ttaagaatga ggtatcagct atggcttatc gcgaccaacc tttaggtgaa ctggcgctct
    13561 ctatccctcg cgcttcggct ctgtttcgta aatatgatat ggattactgc tgtggcggta
    13621 agcagacgct ggcgcgcgcg gcggcacgta aagaactgga tgttgaggtc attgaagctg
    13681 aactggcaaa gcttgctgaa caaccgattg agaaagactg gcgtagcgcc ccgctggcag
    13741 aaatcattga ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag ctgccggagc
    13801 tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc gtgccaaaag
    13861 ggctgacaaa atacctgacc atgctgcatg aagagctttc cagccacatg atgaaagaag
    13921 agcagatcct cttcccgatg atcaaacaag gcatgggcag ccaggcaatg gggccaatca
    13981 gcgtaatgga aagcgagcac gatgaagcgg gcgaactgct ggaagtgatc aaacacacta
    14041 ccaataacgt cacaccgccg ccagaagcct gcaccacctg gaaagcgatg tataacggca
    14101 ttaatgaact gattgatgac ctgatggatc acatcagtct ggaaaacaat gtactgttcc
    14161 cacgcgccct agcgggtgag tgatctaaaa gctggatggc attgcgccat ccagcatgat
    14221 aatgcggtta tttccgcagt tcagcagccc gcttcttacc aataaacagc cagcccaacc
    14281 ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg gcgagtgtca tcttccagtg
    14341 tcagcaacac gaccacgaac acaaagaacg ccatacatac ccagcacatc agcttgccga
    14401 gcggcatctt gtagatcgac ttttcatgca gatgaggacg ctgtttgcgg tacaccaggt
    14461 aagagcaaag gataatcgtc cagacgaaca taaacagaat cgcggaaacg gttgtaatca
    14521 tcgtgaacgc gccaatcacg ctaggattca catacaacat caccacgccg ccgagcagac
    14581 agatacacga gaaggtcaac cctttcgcgg gtactgcgcg cttagaaagt ttagcgaacg
    14641 ctttcggcgc aacaccttcc tgcgccagac caaacagcat acggctggta gagaagacac
    14701 cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg ctggcagccg
    14761 caggcaaacc taccaacaca aacagctcaa cgaacgggct tttctccggg actaccgaac
    14821 tccacggtgt cacggacata atcacaatca gcgcgaagac gtagaacata atgatacgga
    14881 tcggaatgga gttaatcgcg cgtggcagtg atttctccgg atctttggtt tccgcagctg
    14941 ttgtacctac cagctcaatc cccacgaaag cgaaaaccgc tatctggaat ccggcaaaga
    15001 agccacttaa acctttcggg aaccagccgc cgtcattcca caaatgcgcg aatgacgctt
    15061 ccacaccggt cggtgactga aagtgcatcg ccaccatgac caggccgacg acaatcagcg
    15121 acacgatggc gacgattttg atcatcgcaa accagaactc catctcaccg aacattttca
    15181 cggtggcaag gttgagcgtc agcagcagca ctatcaccgc cagcgaggcg acccagtcgg
    15241 agagatcggg gaaccagaac tgagcataag ccgtgatcgc caccacgtct gccataccgg
    15301 ttacaaccca gcagaaccag taagtccagc cggtgaaata tcccgcccac ggccccagta
    15361 aatcggaagc gaagtcacta aaagatttgt attccagatt cgaaagcagc aattccccca
    15421 ttgcccgcat cacgaaaaag agcataaaac caatgatcat ataaacgaaa atgatcgacg
    15481 gcccggcaag gctaatcgtt ttgccggacc ccataaacaa cccagtacca atggcacctc
    15541 caatggcaat aagctgaaca tgtcggtttg tgagattgcg ccgtagcgac tgttcagccg
    15601 gagcctgatc atcggcaacg acttttacct gatctaccat gttttttctt cctgtacctg
    15661 tctgtgttgt tcaggctcta cggcctttag tcagtctatg atacgacgaa tctgtatcgg
    15721 gactcatcga tattaggtaa caatacgcgg gatgaatact aagatttaga tctaatgtta
    15781 attttatgtt taaagtgagt gtcatatcac ggtgataacg cgaaacagct cacaaaaata
    15841 cacgcttacg gggtctttgc aacacaaaat agcgccgttt ggcaaactga agggtttatt
    15901 gctgaatgcc tgctcccctc tggttcgagg ggaacagaat gcgctgctta gaggatttcc
    15961 agcagttcga cttcaaacac cagggtgctg aacggaggga tggatgcacc tgcgccgcgc
    16021 tcgccatatg ccagttcctg cgggatagtc agttcccatt tagaacctac cggcatcaga
    16081 gtcagtgctt caatccagcc agggatcacg ccattaaccg ggaattcagc gggttcacca
    16141 cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt taccggtgta atgaacacga
    16201 acgcggtcgg tacgtgccgg aattgcgcct tcaccctggt tgatcacgcg gaattgcagg
    16261 ccagattcgg tgctattcac accttctttt ttggcgtttt cttccaggta tttcacgcct
    16321 tcagcagcca tcgcctggaa acgctgacga cgaacggcat cggcgcgttc gtggatttca
    16381 cgcagcgcgc gatgcaccac atcaaccggc acagccggat gtttgccttc cagcgcatcg
    16441 gcaatacctg caaccagtgc ttctggcagc agcccttcca ggccagattc actcagttgt
    16501 tgccctacct gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc aaaagttggg
    16561 gtggtcatcg tatttccttt cttagggtat aaaagtagcg ggcagcatat cagccacact
    16621 tctttgggta aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa gaaacaatag
    16681 caatatcccg tatgaatata ataaaggcgg gtttaccacg gacatcatag cgttagctta
    16741 ttcataggct atgattgagg aacaagacgt ggagcaggag gaaaccatgc ccgggcgctt
    16801 tgaactaaaa ccaaccctgg agaaagtctg gcacgcgccg gacaattttc gcttcatgga
    16861 cccgctgccg ccgatgcatc gtcgggggat cattattgcc gccattgtat tggtggtcgg
    16921 atttctgctt ccatctgatg atacgcccaa cgcgtcggtg gtgacgcgtg aagcgcagtt
    16981 agatattcag tcgcaatcac aaccgccgac agaagaacag ccgcaaacta cccaaacgca
    17041 accattccag ccagatagcg gaattgataa tcaatggcgc tcttaccgtg tcgaacccgg
    17101 taaaacgatg gcgcagctgt tccgcgacca cggactgccc gctaccgatg tctatgcaat
    17161 ggcgcaggta gaaggcgcgg gtaagccgct gagtaatttg caaaatgggc agatggtgaa
    17221 aatccgccag aacgccagcg gcgtggtgac gggcttaacc attgatacgg ggaataatca
    17281 acaggtcctg tttacccgcc agccggatgg cagttttatt cgtgcgcggt agatgaaggg
    17341 tcaaccaaaa gccagctaag gattgtctct ttgatgacct tacgtctggc ttccagtgcc
    17401 tggggggaaa ccatactgat atgataaact aggccaagcg tatgctggtt gatcaaataa
    17461 tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc tggcttaaaa
    17521 atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg agcatggtta
    17581 atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt ttcactatgc
    17641 acaattttta aaaaccaagg attagcaata taataatccc atgtaaaatc aataattttc
    17701 tcaatggctt tttgtggcgg aagatctgag aaatcaaggg ccttttcttt ttgtcgaata
    17761 tcgttccagg tatattccag cacttcgata aataaatttt ctttattacc aaagtggtga
    17821 taaaccgtct gtttactaca ccctgctgcc tttacaatat tatctactct ggccccttca
    17881 taaccatatt cggcaaattc attaaccgca catagcagca atttttcctt caggctctgt
    17941 acgttgctgg tatcaatata cataacgctt ccccacgacc tcttcgtggt gactatcata
    18001 acctgatttg atttcagctt cagaaaaaag ggcaaaaact tgtactttca tcaggttatc
    18061 aaatgtcctg tgatcgccat cgcatatcaa ccaactagat ggtttatctt tttatcctcc
    18121 agtcgcaagc taatcatcac caaggaaaca aaattcaaaa aagagagcta ttgtatgaaa
    18181 tccactcgtc ctgttgccgt gattaccgga gccactcgcg gtattggaaa gggctgtgcg
    18241 caggagcttg ctcgtggcgg gtttaacctg ctgattaacg atcgtcccga tgccgacagc
    18301 gtagaaaaac tgcatatcac acaacaagaa tgtttggcgg aaggcgtgga agtgatttgc
    18361 ttccctgctg atgtcggtga tctatctctg catgaggaga tgctcgacgc ggcgcaaaac
    18421 cagtgggggc ggctggactg tctgctcaat aacgcgggga tttcagtgaa aaaacgcggt
    18481 gacctcctcg acctcgaacc agacagcttc gatcaaaaca ttgccattaa cacccgcgcg
    18541 ccgttcttcc ttgctcaggc attcagtaag cgcttactgg cacagccgaa accggaagcg
    18601 gagctgccgc accgctcgat tattttcgtc agttccatca acgccatcat gctggcgatg
    18661 aatcgcggcg aatacaccat tgccaaaacc gccgtttctg ccgctgctcg tctgtttgct
    18721 gctcgccttt gtaatgaaca aattggcgtt tatgaagtgc gccctggtct catcaaaact
    18781 gatatgacta ttccggcaac ggcttattac gacgaactga ttgccaaagg tctggtgccg
    18841 tggggacgtt ggggttatcc ggctgatatt gcttccaccg tccgcgcgat ggcggaaggt
    18901 aaactgattt acacctgtgg tcaggcggtc gcaatcgacg gcggcttaag tatgccgcgc
    18961 ttctgaggct gaatcatgag agatctgtta caacgcccgg atttattcag tatcaatacc
    19021 gcgacactgg gctataaaac gccgctaccc gccattattg atgcctgcgc cgcacgcggt
    19081 attggcgcaa ttgccccctg gcgcagggag ttgcagggtg aagatctcca gcaaatcacc
    19141 cgccagcttg ccgccagtaa catgagcgtt tccggtttgt gccgtagcac ttactacacc
    19201 gcgccgacac tggcggaacg caaactggcg attgatgata accgccgggc gctggacgac
    19261 gcagcggtgc ttaacgccgc ctgttatatg caggtggttg gcggtctgcc aacgggcact
    19321 aaagatctat atgaagcgcg agaacaagtg aaacaaggga ttcgccagtt gctgccacat
    19381 tcgaaagatg tcggcgtgcc aattgcgctg gagccactgc acccgatgac agctgccgat
    19441 cgctcttgcc tgtgcacgtt acgtcaggcg ctggactggt gtgatgaact cgatcccgat
    19501 ggtgagtttg gcctcggcgt ggcggtggat gtttatcacg tctggtggga tccggatctc
    19561 gccagtcaga tcctgcgtgc cggaaaacgc atc
//
LOCUS       DAJTRW010000075        19304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRW010000075 DAJTRW010000000
VERSION     DAJTRW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..2053
                     /gene="pbpC"
                     /locus_tag="OO326_002842"
     CDS             <1..2053
                     /gene="pbpC"
                     /locus_tag="OO326_002842"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5967167.1"
                     /translation="FWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVARLLDPHP
                     KTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAASWAYLGKSPANLS
                     YSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQVKESREEPIWL
                     APRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIV
                     VNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASL
                     LQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNVG
                     LPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMS
                     SGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTG
                     RPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVICWPGGQSLPE
                     GDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAADCPQAKQEMV
                     NVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAE
                     ATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIATVKFVM
                     Q"
     gene            2203..2634
                     /gene="ndk"
                     /locus_tag="OO326_002843"
     CDS             2203..2634
                     /gene="ndk"
                     /locus_tag="OO326_002843"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5967168.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2784..3938
                     /gene="trmG/rlmN"
                     /locus_tag="OO326_002844"
     CDS             2784..3938
                     /gene="trmG/rlmN"
                     /locus_tag="OO326_002844"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5967169.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4223..5236
                     /gene="rodZ"
                     /locus_tag="OO326_002845"
     CDS             4223..5236
                     /gene="rodZ"
                     /locus_tag="OO326_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5967170.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5263..6390
                     /gene="ispG"
                     /locus_tag="OO326_002846"
                     /gene_synonym="gcpE"
     CDS             5263..6390
                     /gene="ispG"
                     /locus_tag="OO326_002846"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5967171.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6493..7767
                     /gene="hisS"
                     /locus_tag="OO326_002847"
     CDS             6493..7767
                     /gene="hisS"
                     /locus_tag="OO326_002847"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5967172.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7785..8405
                     /gene="yfgM"
                     /locus_tag="OO326_002848"
     CDS             7785..8405
                     /gene="yfgM"
                     /locus_tag="OO326_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5967173.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8416..9594
                     /gene="bamB"
                     /locus_tag="OO326_002849"
     CDS             8416..9594
                     /gene="bamB"
                     /locus_tag="OO326_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5967174.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9712..11184
                     /gene="der"
                     /locus_tag="OO326_002850"
     CDS             9712..11184
                     /gene="der"
                     /locus_tag="OO326_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5967175.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11254..11469
                     /locus_tag="OO326_002851"
     CDS             11254..11469
                     /locus_tag="OO326_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5967176.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11466..12836)
                     /gene="xseA"
                     /locus_tag="OO326_002852"
     CDS             complement(11466..12836)
                     /gene="xseA"
                     /locus_tag="OO326_002852"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5967177.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12998..14464
                     /gene="guaB"
                     /locus_tag="OO326_002853"
     CDS             12998..14464
                     /gene="guaB"
                     /locus_tag="OO326_002853"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5967178.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14533..16110
                     /gene="guaA"
                     /locus_tag="OO326_002854"
     CDS             14533..16110
                     /gene="guaA"
                     /locus_tag="OO326_002854"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5967179.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16205..16744)
                     /locus_tag="OO326_002855"
     CDS             complement(16205..16744)
                     /locus_tag="OO326_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5967180.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16760..17278)
                     /locus_tag="OO326_002856"
     CDS             complement(16760..17278)
                     /locus_tag="OO326_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5967181.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKPLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17589..17780)
                     /locus_tag="OO326_002857"
     CDS             complement(17589..17780)
                     /locus_tag="OO326_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5967182.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17798..17950
                     /locus_tag="OO326_002858"
     CDS             17798..17950
                     /locus_tag="OO326_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967183.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18132..19271
                     /gene="pdeF"
                     /locus_tag="OO326_002859"
                     /pseudo
     CDS             18132..19271
                     /gene="pdeF"
                     /locus_tag="OO326_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT         4545 a         4698 c         5250 g         4811 t
ORIGIN      
        1 gttctggaag catccggggg ttaatccatt ctcggtggcg cgcgcagcat ggcaagatct
       61 cacttcggga cgggttattt ccggtggcag cacgctcact atgcaggttg ctcgtctgct
      121 tgatcctcac cccaaaacat ttggtggcaa aattctccag ctctggcgcg cgttgcaact
      181 ggagtggcat ttgtctaagc gtgaaattct gatcttgtat cttaaccgcg ctccgtttgg
      241 cggtacgttg caggggatcg gtgcggcaag ttgggcttat ctcggaaaat cgcctgcgaa
      301 tttaagctat tccgaggcgg caatgctggc ggtgttaccg caagtgccca gccgactgcg
      361 cccggatcgt tggccggagc gtgccgaagc cgcgcgtaat aaagtgctcg aacggatggc
      421 tgcgcaaggt gtgtggtccc gtgagcaggt aaaagagtca agggaagaac ccatctggct
      481 ggcaccccga caaatgccgc aactggcacc gctgttttcg cgcatgatgc tcggtaaaag
      541 caaaagcgac aaaatcgtta ctacgctgga tgccggtctt caacgacgtc tggaagaact
      601 ggcgcaaaac tggaaagggc ggttgccacc gcgcagctca ctggcgatga tcgtggttaa
      661 tcataccgat atgcgtgttc gcggctgggt gggatcggtt gatctcaacg atgattcacg
      721 ctttggtcat gttgatatgg tcaatgcgat ccgatcgcca ggatcggtgc tcaaaccgtt
      781 tgtttatggt ctggcgctgg atgaaggctt gatccacccg gcatcactgc tgcaagacgt
      841 cccccggcgc accggtgatt atcgaccagg taactttgat agcggttttc atggcccgat
      901 caacatgagc gaggcgctgg tgcgctcgct gaacttacct gctgtgcagg tgctggaagc
      961 ctatggaccg aaacggtttg cggcaaagtt acgcaatgtt ggattgccgt tatatttgcc
     1021 caacggcgct gcgccgaatc tttcactcat tctcggcggc gctggtgcaa aactggaaga
     1081 tatggcggca gcgtataccg cgtttgctcg ccacggcaag gcaggcaaat tgcgcttaca
     1141 gcctgacgat ccgctgcttg aacggccttt aatgtcgtcg ggggcggcgt ggatcattcg
     1201 ccggattatg gctgatgaag cgcaaccctt gccggatagt gccttgccgc gcgtcgcccc
     1261 actggcatgg aaaacgggca ccagctatgg ctatcgtgac gcctgggcga ttggggttaa
     1321 cgctcgctat gtcattggga tctggactgg cagacctgac ggcacgcccg ttgttggtca
     1381 gtttggcttt gccagtgccg taccattgtt gaatcaggtc aataatatct tactgtcgcg
     1441 cagtgcgaat ctgccggaag acccgcgccc gaactcagtc actcgtggcg ttatctgctg
     1501 gccgggcgga cagtctttgc cggaaggtga cggtaactgt cgccgccgcc tggcaacctg
     1561 gctgctggat ggcagccagc cgccaacgct gttactgtca gagcaggaag ggattaatgg
     1621 cattcgcttc cccatctggc tggatgacaa agggcagcgc gtcgccgctg actgcccgca
     1681 ggcgaagcaa gaaatggtaa atgtctggcc gctaccgctg gaaccctggc tgcccgcatc
     1741 agaacgtcgc gctgtgcgct taccaccggc ctcgacaatt tgcccgccat acggtcacga
     1801 tgcccagctc cctctgcagc tgacaggcgt tcgcgatggc gcgattatta aacgtttacc
     1861 gggcgcagcg gaagcgactt tgccgttgca atcaagtgga ggggcaggtg aacgctggtg
     1921 gtttctgaat ggcgaaccgt taactgaacg cgggcgcaac gtgaccctgc atttgacgga
     1981 taaaggtgat tatcaattgc tggtgatgga tgatgtgggg caaatcgcga cagtgaaatt
     2041 tgtcatgcaa tagtctgttt tgtccctgat tgttgctaaa actcatttta ttttaaaaaa
     2101 aatgttacct tcatcaatag tcaacggccc tgttgctcat tataatccgc gccatctcgt
     2161 acgctggtac agacaacaac agaacaattt acagaggtaa aaatggctat tgaacgtact
     2221 ttttccatca tcaaaccgaa cgcggtagca aaaaacgtca ttggtaatat ctttgcgcgc
     2281 tttgaagctg cagggttcaa aattgtcggc accaaaatgc tgcatctgac cgttgaacag
     2341 gcacgtggct tttatgctga acacgatgga aaaccgttct ttgatggtct ggttgaattc
     2401 atgacctctg gcccgatcgt ggtttccgtg ctggaaggtg aaaacgccgt tcagcgtcac
     2461 cgcgatctgc tgggcgcgac caatccggca aacgcactgg ctggtactct gcgcgctgat
     2521 tacgctgaca gcctgaccga aaacggtacc cacggttctg attccgtcga atctgccgct
     2581 cgcgaaatcg cttatttctt cggcgaaggc gaagtgtgcc cgcgcacccg ttaataattt
     2641 cgtaaatgcc gcgtgcaaac gtggcatccg tgcgccagaa tttgtacaat gcagcgcccc
     2701 cggacgagca gccgctcacc ggggcgtttc ttttttcaac cctccagggg ccataacgtg
     2761 taataacgag gccggaatag catatgtctg aacaattagt cacacctgaa aacgtcacca
     2821 cgaaagatgg aaaaatcaac ctgctggatc tcaaccgtca gcagatgcgg gagtttttta
     2881 aagatttagg tgaaaaaacc ttccgcgccg atcaggtgat gaagtggatg tatcactatt
     2941 gctgcgacaa ctttgatgag atgaccgaca tcaacaaagt gttgcgcggc aaactgaaag
     3001 aggtggcgga aatccgcgca ccggaagtgg ttgaagaaca gcgttcatcc gacggcacca
     3061 ttaaatgggc gatcgccgtt ggcgatcagc gcgtcgaaac ggtgtatatc ccggaagacg
     3121 accgtgccac gctctgcgtc tcttcgcagg tggggtgtgc gctggagtgt aaattctgtt
     3181 ccaccgccca gcagggcttt aaccgcaacc tgcgggtatc ggaaattatt ggccaggtgt
     3241 ggcgtgcggc gaaaatcgtc ggcgcagcaa aagtcaccgg tcagcgccca atcactaacg
     3301 tcgtgatgat gggcatgggc gagccgctgc tcaacctgaa caacgtcgtt ccggcgatgg
     3361 aaatcatgct tgatgatttc ggttttggcc tgtctaaacg tcgcgtgacg ctttccactt
     3421 ccggtgtagt tccggcgctg gataaactgg gcgatatgat cgacgttgca ctggcaattt
     3481 ccctgcacgc gccgaacgac gaaattcgtg acgaaattgt gccgatcaac aaaaagtaca
     3541 atatcgaaac gttccttgct gcggtgcgcc gttatctgga gaaatccaac gccaatcagg
     3601 gccgagtcac tattgaatac gtgatgcttg accacgttaa cgacggcact gaacacgcgc
     3661 accaactggc ggaactgctg aaagatacgc cgtgtaagat caacctgatc ccgtggaacc
     3721 cgttcccgga cgcgccgtat ggacgcagtt cgaacagccg tatcgaccgt ttctcaaaag
     3781 tgctgatgag ctacggtttc accactattg tgcgtaaaac gcgtggtgat gatatcgatg
     3841 ctgcctgtgg tcagttggcg ggcgatgtta tcgaccgtac gaaacgtact ctgcgtaaac
     3901 ggatgcaggg tgaagccatc gatattaaag cggtctgata gccattacgc cacggttact
     3961 ttatgtaccg tggcgtaatc ttttatgaat gaagtattaa gttgcaattg tctggtcaat
     4021 attaacggtg cgtttttgct gactttaagg cagtatgtaa cgatgcaaca gtaagttagc
     4081 ctcagtgaat gtgggctttg tcacgagcac acagacggtc ttatactgta tgataacggt
     4141 taacttaacg gatgtttcgc ggtgtgggtg agcatgatgg ttcaccggca tctcaattct
     4201 catttaaacg tacctgcagc gaatgaatac tgaagccacg cacgaccaaa atgaagcact
     4261 tactaccggc gctcgcctgc gtaatgctcg cgaacaacta ggacttagtc agcaggccgt
     4321 tgccgagcga ctttgcctga aggtttccac ggtacgcgac attgaagaag ataaggcacc
     4381 cgccgatctt gcttcaacat tcctgcgcgg atatatccgc tcttatgcgc gtctggtaca
     4441 tattccagaa gaagaactgc tgccagggct ggaaaagcag gctccacttc gggctgcaaa
     4501 agttgcgcca atgcagagtt tttccctcgg taaacgccgc aaaaaacgcg acggctggct
     4561 aatgaccttc acctggctgg tgttgtttgt ggttatcggc ctgagcggtg cctggtggtg
     4621 gcaagaccac aaagctcagc aggaagagat caccactatg gccgatcaat cttcggcgga
     4681 actgagcagt aatagcgagc aggggcagag tgttccgtta aatacgtcga caactacaga
     4741 cccggctaca acgtccacgc cgccagcgtc tgtggatact accgcaacca acacgcaaac
     4801 acctgccgta actgcgccag caccagctgt tgatccgcaa cagaatgcgg ttgtttcgcc
     4861 ttcgcaggca aatgttgata ccgccgcgac cccggtacca acggcagcaa caacgccaga
     4921 tggtgctgcg ccgttgccaa ccgatcaggc tggcgtgacc acgccggcgg ctgatccgaa
     4981 tgcgctggtg atgaacttta ctgccgattg ctggctggag gtcactgatg ctaccggtaa
     5041 aaaattgttt agcgggatgc agcgtaaaga cggtaatttg aatttaaccg gccaggcacc
     5101 gtataaactg aaaattggtg cgccagccgc agtacagatc cagtatcaag ggaaacctgt
     5161 cgatctgagt cgttttatca gaactaacca ggttgcgcgt ctgaccctca atgccgaaca
     5221 atcaccggcg cagtaacaga cgggtaacgc gggagatttt tcatgcataa ccaggctcca
     5281 attcaacgta gaaaatcaac acgtatttac gttgggaatg tgccgattgg cgatggtgct
     5341 cccatcgccg tacagtccat gaccaatacg cgtacgacag atgtcgaagc aacggtcaat
     5401 caaatcaagg cgctggaacg cgttggcgct gatatcgtcc gtgtctccgt accgacgatg
     5461 gacgcggcag aagcgttcaa actcatcaaa cagcgggtta acgtgccgct ggtggctgac
     5521 atccacttcg actatcgcat tgcgctgaaa gtagcggaat acggcgtcga ttgtctgcgt
     5581 attaaccctg gtaatatcgg taatgaagag cgtattcgca tggtggttga ctgtgcgcgc
     5641 gataaaaaca ttccgatccg tattggcgtt aacgccggat cgctggaaaa agatctgcaa
     5701 gaaaagtatg gcgaaccgac gccgcaggcg ttgctggaat ccgccatgcg tcatgttgat
     5761 catctcgatc gcctgaactt cgatcagttc aaagtcagcg tgaaagcgtc tgacgtcttc
     5821 ctcgctgttg agtcttatcg tttgctggca aaacagatcg atcagccgct gcatctgggg
     5881 atcaccgaag ccggtggtgc acgcagcggg gcagtaaaat ccgccattgg tttaggtctg
     5941 ctgctgtcgg aaggcatcgg cgacacgctg cgtgtatcgc tggcggccga tccggtcgaa
     6001 gagatcaaag tcggtttcga tattttgaaa tcgctgcgta tccgttcgcg agggattaac
     6061 ttcatcgcct gcccgacctg ttcgcgtcag gaatttgatg ttatcggtac ggttaacgcg
     6121 ctggagcaac gcctggaaga tatcatcact ccgatggacg tttcgattat cggctgcgtg
     6181 gtgaatggcc caggtgaggc gctggtttct acactcggcg tcaccggcgg caacaagaaa
     6241 agcggcctct atgaagatgg cgtgcgcaaa gaccgtctgg acaacaacga tatgatcgac
     6301 cagctggaag cacgcattcg tgcgaaagcc agtcagctgg acgaagcgcg tcgaattgac
     6361 gttcagcagg ttggaaaaat aataacgtga tgggaagcgc ctcgcttccc gtgtatgatt
     6421 gaacccgcat ggctcccgaa acattgaggg aagcgttgag ggttcatttt tatattcaga
     6481 aagagaataa acgtggcaaa aaacattcaa gccattcgcg gcatgaacga ttacctgcct
     6541 ggcgaaacgg ccatctggca gcgcattgaa ggcacactga aaaacgtgct cggcagctac
     6601 ggttacagtg aaatccgctt gccgattgta gagcagaccc cgctattcaa acgtgcgatt
     6661 ggtgaagtca ccgacgtggt tgaaaaagag atgtacacct ttgaggatcg caatggcgac
     6721 agcctgactc tgcgccctga agggacggcg ggctgtgtac gcgccggcat cgagcatggt
     6781 cttctgtaca atcaggaaca gcgtctgtgg tatatcgggc cgatgttccg tcacgagcgt
     6841 ccgcagaaag ggcgttatcg tcagttccat cagttgggct gcgaagtttt cggtctgcaa
     6901 ggtccggata tcgacgctga actgattatg ctcactgccc gctggtggcg tgcgctgggt
     6961 atcttcgaac acgtaactct tgagctgaat tctatcggtt cgctggaagc acgcgccaat
     7021 taccgcgatg cgctggtggc attccttgag cagcataaag aaaagctgga cgaagactgc
     7081 aaacgccgca tgtacactaa cccgctgcgc gtgctggatt caaaaaatcc ggaagtgcag
     7141 gcgcttctca acgacgctcc ggcattaggt gattatctgg acgaggaatc tcgtgagcat
     7201 tttgccggtc tgtgcaaact gctggagagc gcggggatcg cttacaccgt aaaccagcgt
     7261 ctggtgcgtg gtctggatta ctataaccgt accgttttcg agtgggtgac taacagtctc
     7321 ggctcccagg gcaccgtgtg tgcaggcggt cgttatgacg gtcttgtgga acaactgggc
     7381 ggtcgtgcaa caccggctgt cggttttgcg atgggcctcg aacgtcttgt attgttagta
     7441 caggccgtta atccggaatt taaagccgat cctgttgtcg atatatacct ggtggcttca
     7501 ggtgctgata cacaatctgc ggctatggca ttagctgagc gtctgcgtga tgaattactg
     7561 ggcgtgaaat tgatgaccaa ccacggcggc ggcaacttta agaaacagtt tgcccgtgct
     7621 gataaatggg gtgcccgcgt tgctgtggtg ctgggtgagt ctgaagtggc taacggcaca
     7681 gcagtagtga aggatttgcg ctctggtgag caaacggcag ttgcgcagga tagcgtagcc
     7741 gcgcatttgc gcacgttact gggttaagga aggagaagga cagcgtggaa atttacgaga
     7801 acgaaaacga ccaggtagaa gcggttaaac gcttttttgc tgaaaatggc aaagcactgg
     7861 ctgttggggg gattttgggc gttggcgcac tgactggctg gcgctactgg aacagccatc
     7921 aggttgattc tgcacgctcc gcttctcttg cctatcaaaa tgcggttacc gcagtgagcg
     7981 aaggcaaacc ggatagcatc ccggcggcgg aaaaatttgc tgctgaaaat aaaaatactt
     8041 atggtgcgct ggcttctttg gaacttgcgc agcaatttgt tgacaaaaat gaactagaga
     8101 aagctgccgc ccagttacaa caggggctgg cagacacgag cgatgaaaat ctcaaagccg
     8161 tgataaatct gcgtcttgct cgcgttcagg tacagctcaa gcaggctgat gccgcgctga
     8221 aaacccttga taccatcaaa ggtgaagggt gggctgccat tgttgccgac ttgcgtggtg
     8281 aagcattgct gagcaaaggt gataagcaag gtgcgcgtag tgcatgggaa gcaggcgtga
     8341 aaagcgatgt tactccggca ctgagcgaaa tgatgcagat gaaaattaat aatttgtcca
     8401 tctgagaggg acccgatgca attgcgtaaa ttactgctgc caggactgct ttccgttacc
     8461 cttttaagcg gctgttcgct gtttaacagc gaagaagatg tggtaaagat gtccccattg
     8521 ccaaccgttg aaaaccagtt tacgccgacc acggcgtgga gcacttccgt tggtagcggc
     8581 attggcaact tctattccaa tcttcatccg gcactggcgg acaacgttgt ctatgcagcg
     8641 gaccgcgctg gtttggtaaa agcgctgaat gcggatgatg gcaaagaaat ctggtctgtc
     8701 agcctggccg agaaagatgg ctggttctct aaagagcctg cattactttc tggcggtgtg
     8761 accgtgtctg gtgggcatgt ctacattggc agcgaaaagg cgcaggttta cgcgctgaat
     8821 accagcgatg gtactgtggc atggcaaact aaagtcgcgg gtgaagcact ttcgcgcccg
     8881 gtggtcagcg acggtctggt gttaatccac accagtaacg gtcagttaca agcgctgaac
     8941 gaagctgacg gcgctgtcaa atggacagtt aacctcgata tgccttcgct ctctttgcgt
     9001 ggcgagtctg cgccggcaac ggcttttggt gcggccgtcg tgggtggcga taatggtcgc
     9061 gtcagcgcag tgctgatgga acagggccag atgatttggc agcagcgtat ttcccaggcg
     9121 accggttcta ccgaaattga ccgtctgagc gatgttgaca cgactcccgt cgttgttaac
     9181 ggcgttgttt tcgcgctggc ctataatggt aacctgacgg cgcttgatct gcgcagtggt
     9241 cagattatgt ggaaacgcga actgggttcg gtgaatgatt tcatcgtcga tggcaatcgc
     9301 atctatctgg tcgatcaaaa tgaccgggtg atggcgttga ccattgatgg cggcgttacg
     9361 ctgtgggcac aaagcgatct gctgcatcgc ctgctgactt ctccggtgct gtataatggc
     9421 aacctggtgg tcggtgacag tgaaggttat ctgcactgga ttaacgtcga agatggtcgt
     9481 ttcgttgccc agcaaaaagt tgatagttcc ggtttccaga ctgaaccggt tgccgctgac
     9541 ggcaaactgc tgatccaggc aaaagacgga accgtgtact ctattacacg ttaatcgtct
     9601 ctgtcgttca ctttgaaaac ggctcctgga caggggccgt tttcctgttt ttaacaacga
     9661 cgcgaatata gcgtgcgttg tctgatgatt tataaaaatg aggctttaaa catggtacct
     9721 gtggtcgcgc ttgtcgggcg ccctaacgta ggaaaatcca cgttatttaa ccgtctaact
     9781 cgcacccgag atgcgctggt tgcggatttc ccgggtctga ctcgtgaccg taagtacggt
     9841 cgtgcggaaa ttgaaggccg tgagtttatc tgtattgata ccggcgggat tgatggcaca
     9901 gaagacggtg tagaaacccg catggcggaa cagtcgctgc tggcgattga agaagcggac
     9961 gtcgtactgt ttatggtgga tgcgcgcgcg ggcctgatgc cggcagatga agcgattgcc
    10021 aaacatctgc gctcccgtga aaaaccgacc ttcctggtgg taaacaaaac tgacggtctg
    10081 gatcccgatc aggcagtggt tgatttctac tcgcttggtt taggtgaaat ctacccgatc
    10141 gccgcgtctc acggtcgtgg cgtattaagt ctgctggagc atgtgctgct gccgtggatg
    10201 gaagatctcg caccgcaaga ggaagtcgac gaagacgctg aatactgggc gcaatttgaa
    10261 gcggaagaga acggcgaaga agaagaggaa gacgacttcg acccgcaaag tctgccgatc
    10321 aaactggcga ttgtgggtcg tccgaacgta ggtaagtcta cactcactaa ccgtattctt
    10381 ggagaagagc gcgttgttgt ttacgacatg cctggcacga cgcgtgacag catctacatc
    10441 ccaatggaac gcgatggacg tgagtatgtg ctcattgaca ccgctggcgt acgtaaacgc
    10501 ggcaaaatca ccgatgctgt agagaaattc tccgtaatca aaacgttgca ggccattgaa
    10561 gacgccaacg tggtgatgtt agtgattgat gcgcgcgaag gtatttccga tcaggatctc
    10621 tcgctgctgg gctttattct caatagtggg cgctcacttg tcattgtggt gaataagtgg
    10681 gatggcctga gccaggaagt gaaagagcag gtgaaagaaa cgctggactt ccgtctgggc
    10741 tttatcgatt ttgctcgtgt gcactttatc tctgccttgc acggcagtgg tgttggtaac
    10801 ttgtttgaat cagtacgtga agcgtatgac agctccaccc gtcgtgtggg gacctctatg
    10861 ctgacgcgca tcatgacgat ggctgttgaa gatcaccaac cgccgctggt acgcggtcgt
    10921 cgtgtgaagc tgaaatatgc ccacgctggt ggttataacc cgccgattgt ggtgattcac
    10981 ggtaatcagg tgaaagacct gcctgattcc tacaagcgct acttgatgaa ctacttccgc
    11041 aaatcgctgg acgtaatggg atcgccgatt cgtattcagt tcaaagaagg ggaaaacccg
    11101 tatgcgaaca agcgtaacac cctgacgcca acccagatgc gtaaacgtaa gcgtctgatg
    11161 aagcacatca agaaaaataa ataattatcc gtaagtattt tatctgccat tccctctatg
    11221 aatgtagagg gaatggctac cggaggaatt catatggaac tgcattgtcc aaaatgtcag
    11281 catgtgcttg atcaggataa cggccatgcg cgttgcccat cctgcgggga atttattgaa
    11341 atgaaagctc tttgtccaga ctgccatcaa ccgttgcagg tgttaaaagc ctgcggtgca
    11401 gtagattact tctgtcagca cgggcatggc ttgatatcga aaaaacgcgt tgaattcgtg
    11461 ctggcttaat gaaccttttt acgcgatttt tttaccggct ggatgttttt tacttcactt
    11521 tctatccagc cgtcttccag acgtgtggtt agcatttcac ccgttttaac ttgcttcact
    11581 tttttcagta cattgccgtc agtagcagta gtaacgctgt atccacgcgc cagcgttgac
    11641 agtgggctta cggcttcgag gtgcgttact gcattaccga aacgttcacg cgtggcgcta
    11701 agctgtgcgc gcaggatttc tgctaaacga tattccagtt gctgaatgcg cgtttgcgtg
    11761 cgatgaatct tcggctgtgg attttgctga ttcagccgct gtgttaatcg ctgctgctgt
    11821 tgcccggcac gcttaagctg attttccagc gcaaagctca tccgtttttg caggcgttca
    11881 agcatggttt gctggcgtgc cagccggagc tgcggatgct gctgctgcaa gcgatgatga
    11941 atctgcgtaa agcgacgtgt acggttggcg agataataat ccatcgccat ctccagccgt
    12001 tgacgggccg attgcacctg gcgcagtaac tcttgctgat tacggctcac tacttcagcg
    12061 gcggcagacg gcgttggcgc acgcagatcg gcaacaaaat cggcaatggt cacatccgtc
    12121 tcatgcccga cggcgctgac gaccggaatg cggctggcaa aaatcgcccg cgctacgcgt
    12181 tcgtcgttaa aactccataa atcttccagc gaaccgccgc cgcgcccaac gatcaacacg
    12241 tcgcactcat tgcgctgatt cgccagttca atggcgcgaa caatttgccc cggcgcgtca
    12301 tcgccctgaa cggaggtggg gtagatgatc accggtagag aaggatcgcg acgttttaac
    12361 acatgcaaaa tatcatgtag cgcagcaccg gtttttgagg tgatcacacc aacgcaatgc
    12421 gcaggggagg gaagtggttt tttgtattgc tgatcgaaca aacattcagc ctgcaacttc
    12481 gctttgagct gttcgtactt ctgttgcagc agcccttcac cggccggctg catactctca
    12541 acgattatct ggtagtcgcc gcgcggctcg tagagcgtaa tattggcgcg aactaaaact
    12601 tgttgcccat gctgcggacg gaaggtgacc cggcggttgc tgttgcggaa catcgcgcag
    12661 cgtacctggg cggtgtcgtc tttgagtgta aagtaccagt gaccggaagc cggttgtgtg
    12721 aaattagaga tttcgccgct gatccaaacc tgtcccatct catgctcaag cagcagacga
    12781 accgtttgat tcaggcgact aacggtaaaa attgcagggg attgagaagg taacatgtga
    12841 gcgagatcaa attctaaatc agcaggttat tcagtctata gtaacccgcc cttcggggat
    12901 agcaagcatt ttttgcaaaa aggggtagat gcaatcggtt acgctctgta taatgccgcg
    12961 gcaatattta ttaaccactc tggtcgagat attgcccatg ctacgtatcg ctaaagaagc
    13021 tctgacgttt gacgacgttc tcctcgttcc tgctcattct accgttctgc cgaatactgc
    13081 tgacctcagc acccagctga cgaaaactat tcgtctgaat atccctatgc tttccgcagc
    13141 aatggatacc gtaacggaag cgcgcctggc tattgctctg gctcaggaag gcggtatcgg
    13201 ctttatccac aaaaacatgt ccattgaacg ccaggcagaa gaagttcgcc gtgtgaaaaa
    13261 acacgaatct ggtgtggtga ctgatccgca gactgttctg ccaaccacga cgctgcgcga
    13321 agtgaaagaa ctgaccgagc gtaacggttt tgcgggctat ccggtcgtta ccgaagaaaa
    13381 cgaactggtg ggtattatca ccggtcgtga cgtgcgtttt gttaccgacc tgaaccagcc
    13441 ggttagcgtt tacatgacgc cgaaagagcg tctggtcacc gtgcgtgaag gtgaagcccg
    13501 tgaagtggtg ctggcaaaaa tgcacgaaaa acgcgttgaa aaagcgctgg tggttgatga
    13561 cgaattccac ctgatcggca tgatcaccgt gaaagacttc cagaaagcgg aacgtaaacc
    13621 gaacgcttgt aaagacgagc aaggccgtct gcgtgttggt gctgcagttg gcgcaggtgc
    13681 gggtaacgaa gagcgtgttg acgcgctggt tgccgctggc gttgacgttc tgctgatcga
    13741 ctcctcccac ggtcactcag aaggcgtttt gcagcgtatc cgtgaaaccc gtgctaaata
    13801 tccggatctg caaatcatcg gcggcaacgt ggcaacagct gcaggtgcac gcgctttggc
    13861 agaagctggt tgcagcgcgg ttaaagtcgg tatcggccct ggttctatct gtacgactcg
    13921 tatcgttact ggcgtaggtg ttccgcagat cactgccgtt gctgacgcag ttgaagccct
    13981 ggaaggcacc ggtattccgg ttatcgcaga tggtggtatt cgcttctccg gcgacatcgc
    14041 caaagctatc gccgctggcg caagcgcggt gatggtaggt tccatgctgg cgggtactga
    14101 agaatctccg ggtgaaatcg aactctacca gggccgttct tataaatctt accgtggtat
    14161 gggttccctg ggcgcgatgt ccaaaggttc ctctgaccgt tatttccaga gcgataacgc
    14221 tgccgacaaa ctggtgccgg aaggtatcga aggtcgcgta gcttataaag gtcgcctgaa
    14281 agagatcatt caccagcaga tgggcggcct gcgctcctgt atgggtctga ccggctgtgg
    14341 tactatcgac gaactgcgta ctaaagcgga gtttgtacgt atcagcggtg cgggcattca
    14401 ggaaagccac gttcacgacg tgaccattac taaagagtcc ccgaactacc gtctgggctc
    14461 ctgattctct tcgcccgatt tcatgtcggg cgatttatat tatctgtttc acttgcctcg
    14521 gaataagcgt caatgacgga aaacattcat aagcatcgca tcctcattct ggacttcggt
    14581 tctcagtaca ctcaactggt tgcgcgccgc gtgcgtgagc tgggtgttta ctgcgaactg
    14641 tgggcgtggg atgtgacaga agcacaaatt cgtgacttca atccaagcgg aattattctt
    14701 tccggcggcc cggaaagtac tactgaagaa aacagtccgc gtgcgccgca gtatgtcttt
    14761 gaagcaggcg taccgatatt cggcgtttgc tatggcatgc agaccatggc aatgcagttg
    14821 ggcggtcacg ttgaagcctc taacgaacgt gaatttggct acgcgcaggt agaagtcgta
    14881 aacgacagcg cactggttcg cggtatcgaa gatgcgctga ccgcagacgg taaaccgctg
    14941 ctcgatgtct ggatgagcca cggcgataaa gttaccgcta tcccgtccga cttcgtcacc
    15001 gtagccagca ccgaaagctg cccgtttgcc attatggcta acgaagaaaa acgcttctat
    15061 ggcgtacagt tccacccgga agtgactcac acccgccagg gtatgcgcat gctggagcgt
    15121 tttgtgcgtg atatctgcca gtgtgaagcc ctgtggacgc cagcgaaaat tatcgacgat
    15181 gctgtagctc gcatccgcga gcaggtaggc gacgataaag tcatcctcgg cctctctggt
    15241 ggtgtggatt cctccgtaac cgcaatgctg ctgcaccgtg ctatcggtaa aaacctgact
    15301 tgcgtattcg tcgacaacgg cctgctgcgt ctcaacgaag cagagcaggt tctggatatg
    15361 tttggcgatc actttggtct taacattgtt cacgtaccgg cagaagatcg cttcctgtca
    15421 gcgctggctg gcgaaaacga tccggaagca aaacgtaaaa tcatcgggcg cgttttcgtt
    15481 gaagtattcg atgaagaagc gctgaaactg gaagacgtga agtggctggc gcagggcacc
    15541 atctaccctg acgttatcga atctgcggct tctgcaaccg gtaaagcaca cgtcatcaaa
    15601 tctcaccaca acgtgggcgg cctgccgaaa gagatgaaga tgggcctggt tgaaccgctg
    15661 aaagagctgt tcaaagacga agtgcgtaag attggtctgg agctgggcct gccgtacgac
    15721 atgctgtacc gtcatccgtt cccgggacca ggccttggcg ttcgtgttct gggtgaagtg
    15781 aagaaagagt actgtgacct gctgcgccgt gctgacgcca tcttcattga agaactgcgt
    15841 aaagcggacc tgtacgacaa agtcagccag gcgttcaccg tcttcctgcc agtacgttcc
    15901 gttggcgtaa tgggcgatgg tcgtaagtat gactgggttg tctctctgcg tgctgtcgaa
    15961 accatcgact ttatgaccgc acactgggcg catctgccgt acgatttcct cggtcgcgtt
    16021 tccaaccgca ttatcaatga agtgaacggt atttcccgcg tggtgtatga catcagcggc
    16081 aagccgccag ctaccattga gtgggaatga ttttgatccc tgcactatga atgaacaaaa
    16141 ccctccgtta ctacagaggg ttttttatct tcaagaatca taggcttgaa gttactaaca
    16201 tcgattaatt aaaccagctg tccgatttgt tctcttctgc tttgcccacg cttttcatca
    16261 gatcgcgacc gccttcagtc atatttctgt ttgcgtcagc ttcagattgc accacatcgg
    16321 tttgcgcagc tttgtgcttc agttcctgat cgataaattc gttttcgcgc ttaacgcggg
    16381 cttcttcttt cgccagcgcc agtttttgtt tctgaatctc taagctgcgt agctcatctt
    16441 cataactttg atcgcgtttt ttgtccgcag tggcttcggc gtccagttta tcctgacgag
    16501 ctttcttatt tgccgctgcc gttgccgctc ttttgttcgc ggcggcctgg gcatttgcgc
    16561 gacgttgctt ctcttgctgg atttccctgt tgcgctccgc gacccattcg tcatgctgcc
    16621 tttgctcttc atttttacct tgctgttccg cttctgcgac agcagagagt tgatcctgca
    16681 atgatgaggc tatagccgga tagcttaagg aggctaagat ggcgcaaaga aaaactttct
    16741 tcatgactcc tcctgattat tagctctttt caggacattt ggtatttggc tgaatacgcg
    16801 tttcgttata cgttgtggta ataacaacgg ctaaacctgt cgtaaactgg cactctttac
    16861 cctcctgggt ggaggtatac actttggtgc cttccttata tgttaaagaa acaccttcca
    16921 ctaagggttt atcattcacc atagaacccg ctgccgcgcc tacagctccg ccgccaactg
    16981 cccctgccgt cgttccggaa ttgctgccag aaccgacgtt gtggccgata acaccgccag
    17041 cgactgcgcc aataagcgcg ccgaaggctt gtgcgttccg tttattttgg gagttgtcta
    17101 cggcaacttt tgcgggaaga atggaaataa tattaacggt tttagtttct tgtttggtat
    17161 tcagttgatc ggtttgataa acatcggcgg catgatcgtc agcatttgac tggcatcctg
    17221 ccagagtgaa tgacgctaac attgccacag gcagaagaca ttttttaaat ttcatcacta
    17281 ttccttgtta tacacattac gaaaaataag acatttaaga tcatcaaaag aggaaagtaa
    17341 atgtattgcg tataaaggga tagattaaat ttattaaaat tcacgcaggg aataatttat
    17401 aaaaatatat cattacaaca tatatattaa ttttatattt atttcgcagc caaacaggat
    17461 aaacatcagt caggacaaag acgtaaaatt taagatgaaa gaatatctgc cgctttgata
    17521 atcccaaaga gcaaaagcat ccgacagcga agtgccgtta tgttctgtga aagtggagaa
    17581 ggtaaccgct aacgttgtac cggtgattcg acggagagtg tggattgctg agcttctttt
    17641 ttgctctgat ggtgctgcca ggcaccgacg gacgagtaga taaaacggcc aaagaagaag
    17701 ataaagctga tgagcagtac gatacgggtc atgcgactgt taaatcggtg tcgttttcgc
    17761 atactagttg cctgactcac aaaaggttcc ttgaagtatg tcccacgctg tggacggtac
    17821 ttacattaag gcacaacagg acaaaatggt caattcttcg ttatgtaaaa aagcgtcagt
    17881 ggatacatat tttaatgtta tggaagttaa tttaattatt tacaatgatg atgtaataat
    17941 gatgaattaa catgataaac gtaataattc attaatcagg gttattttgt ttgatatata
    18001 tcaattggaa ctttcatatg acacttagaa tcaatgctct ctatagtgat gaataatcat
    18061 cattcgaagt caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct
    18121 gctgagcatc tatgaaactg aatgcaactt atataaaaat acgtgataaa tggtgggggc
    18181 ttccgctgtt cctgccttct ttaatcttgc ccattttcgc ccacattaat actttcgcgc
    18241 atatttcttc cggtgaggtt tttctctttt atctgccttt ggcactgatg atcagcatga
    18301 tgatgttttt cagctgggcg gcattgccag ggatcacctt agggattttt gtccgcaaat
    18361 atgcagagct gggtttttac gaaacgctct cattaacggc taattttatt atcattatca
    18421 ttctctgttg gggcggttac agggtcttta ctccccggcg taacaacgtt tcacatggtg
    18481 atacccgttt aatttcccag cgtatattct ggcagatagt gtttcctgca acgctgtttc
    18541 tgatactttt ccagtttgct gcatttgtag gattactggc gagcagagaa aatctggtcg
    18601 gtgtcatgcc ttttaacctc gggactttaa tcaattatca ggccttactg gtgggtaatc
    18661 tgatcggtgt cccgctgtgc tacttcatca ttcgggtagt gcgaaatcca ttttatttac
    18721 gtagctatta ttcgcaatta aaacagcagg ttgatgccaa agtcaccaaa aaagagttcg
    18781 agctctggct actggcatta ggtgctttat tgttgctgtt atgcatgccg ttaaatgaaa
    18841 aaagcacaat ttttagcacc aattatacct tgtcattatt gctgcccctg atgatgtggg
    18901 gagcaatgcg ctatggttat aagctgattt cgctgctctg ggcggtcgtg ttgatgatca
    18961 gcatccacag ctatcaaaat tacattccca tttatcctgg ctataccacg cagctgacca
    19021 taacctcctc cagttatctg gtattctctt ttattgtcaa ttatatggct gtactggcaa
    19081 ctcgtcagcg agcggtagtc agacgcattc agcggcttgc gtatgtggac ccggtggttc
    19141 atctgccaaa tgttcgcgcc ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt
    19201 gttatttacg catccctggc atggaaatgc tggttaagaa ctatggcatc atgctgcgga
    19261 ttcaatatga ggtgtactgg caatagcgga cactaccatt tgtt
//
LOCUS       DAJTRW010000076        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRW010000076 DAJTRW010000000
VERSION     DAJTRW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(139..3654)
                     /locus_tag="OO326_002860"
     CDS             complement(139..3654)
                     /locus_tag="OO326_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5967184.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO326_002861"
     CDS             complement(4107..5369)
                     /locus_tag="OO326_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5967185.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO326_002862"
     tRNA            complement(5567..5642)
                     /locus_tag="OO326_002862"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO326_002863"
     CDS             complement(5748..6455)
                     /locus_tag="OO326_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5967186.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO326_002864"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO326_002864"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5967187.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO326_002865"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO326_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5967188.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO326_002866"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO326_002866"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5967189.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO326_002867"
     CDS             complement(11639..11914)
                     /locus_tag="OO326_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5967190.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO326_002868"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO326_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5967191.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO326_002869"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO326_002869"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5967192.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO326_002870"
     CDS             13874..14200
                     /locus_tag="OO326_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5967193.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO326_002871"
     CDS             14384..15103
                     /locus_tag="OO326_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5967194.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO326_002872"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO326_002872"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5967195.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO326_002873"
     CDS             16442..17404
                     /locus_tag="OO326_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5967196.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO326_002874"
     CDS             complement(17505..>18803)
                     /locus_tag="OO326_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5967197.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTRW010000077        18068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRW010000077 DAJTRW010000000
VERSION     DAJTRW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            256..780
                     /locus_tag="OO326_002875"
     CDS             256..780
                     /locus_tag="OO326_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5967198.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO326_002876"
     CDS             861..1535
                     /locus_tag="OO326_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5967199.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4013
                     /locus_tag="OO326_002877"
     CDS             1551..4013
                     /locus_tag="OO326_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5967200.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4029..5062
                     /locus_tag="OO326_002878"
                     /pseudo
     CDS             4029..5062
                     /locus_tag="OO326_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO326_002879"
     CDS             complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO326_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5967201.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO326_002880"
                     /pseudo
     CDS             complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO326_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6653..6925
                     /gene="rcnR"
                     /locus_tag="OO326_002881"
     CDS             6653..6925
                     /gene="rcnR"
                     /locus_tag="OO326_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5967202.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7017..7113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7148..7936
                     /gene="thiM"
                     /locus_tag="OO326_002882"
     CDS             7148..7936
                     /gene="thiM"
                     /locus_tag="OO326_002882"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5967203.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7933..8733
                     /gene="thiD"
                     /locus_tag="OO326_002883"
     CDS             7933..8733
                     /gene="thiD"
                     /locus_tag="OO326_002883"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5967204.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8798..9619
                     /locus_tag="OO326_002884"
     CDS             8798..9619
                     /locus_tag="OO326_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5967205.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9667..10413
                     /locus_tag="OO326_002885"
     CDS             9667..10413
                     /locus_tag="OO326_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5967206.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10387..11352)
                     /locus_tag="OO326_002886"
     CDS             complement(10387..11352)
                     /locus_tag="OO326_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5967207.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11349..12352)
                     /locus_tag="OO326_002887"
                     /pseudo
     CDS             complement(11349..12352)
                     /locus_tag="OO326_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12392..13444
                     /gene="fbaB"
                     /locus_tag="OO326_002888"
     CDS             12392..13444
                     /gene="fbaB"
                     /locus_tag="OO326_002888"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5967208.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13752..14606
                     /gene="gatY"
                     /locus_tag="OO326_002889"
     CDS             13752..14606
                     /gene="gatY"
                     /locus_tag="OO326_002889"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5967209.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14635..15897
                     /gene="gatZ"
                     /locus_tag="OO326_002890"
     CDS             14635..15897
                     /gene="gatZ"
                     /locus_tag="OO326_002890"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5967210.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15907..16359
                     /gene="gatA"
                     /locus_tag="OO326_002891"
     CDS             15907..16359
                     /gene="gatA"
                     /locus_tag="OO326_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5967211.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16390..16674
                     /gene="gatB"
                     /locus_tag="OO326_002892"
     CDS             16390..16674
                     /gene="gatB"
                     /locus_tag="OO326_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5967212.1"
                     /translation="MKRKIIVACGGAVATSAMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16678..18033
                     /locus_tag="OO326_002893"
     CDS             16678..18033
                     /locus_tag="OO326_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5967213.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4725 a         4112 c         4571 g         4660 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2641 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2701 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2761 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2821 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2881 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2941 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     3001 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     3061 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     3121 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3181 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3241 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3301 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3361 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3421 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3481 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3541 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3601 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3661 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3721 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3781 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3841 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3901 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3961 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     4021 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     4081 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     4141 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4201 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4261 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4321 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4381 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4441 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4501 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4561 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4621 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4681 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4741 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4801 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4861 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaagtat acacaaattt
     4921 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4981 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     5041 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     5101 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     5161 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5221 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5281 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5341 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5401 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5461 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5521 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5581 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5641 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5701 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5761 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5821 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5881 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5941 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     6001 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     6061 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     6121 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6181 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6241 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6301 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6361 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6421 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6481 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6541 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6601 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6661 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6721 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6781 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6841 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6901 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6961 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     7021 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     7081 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     7141 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7201 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7261 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7321 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7381 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7441 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7501 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7561 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7621 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7681 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7741 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7801 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7861 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7921 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7981 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     8041 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     8101 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     8161 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8221 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8281 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8341 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8401 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8461 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8521 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8581 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8641 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8701 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8761 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8821 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8881 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8941 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     9001 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     9061 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     9121 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9181 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9241 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9301 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9361 gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9421 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9481 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9541 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9601 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9661 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9721 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9781 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9841 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9901 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9961 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    10021 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
    10081 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    10141 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10201 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10261 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10321 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10381 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10441 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10501 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10561 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10621 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10681 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10741 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10801 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10861 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10921 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10981 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    11041 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    11101 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    11161 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11221 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11281 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11341 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11401 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11461 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11521 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11581 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11641 tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11701 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11761 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11821 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11881 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11941 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    12001 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    12061 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    12121 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12181 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12241 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12301 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12361 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12421 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12481 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12541 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12601 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12661 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12721 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12781 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12841 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12901 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12961 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    13021 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    13081 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    13141 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13201 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13261 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13321 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13381 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13441 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13501 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13561 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13621 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13681 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13741 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13801 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13861 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13921 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13981 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    14041 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    14101 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    14161 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14221 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14281 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14341 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14401 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14461 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14521 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14581 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14641 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14701 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14761 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14821 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
    14881 attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg
    14941 atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat
    15001 cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg
    15061 gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag
    15121 caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt
    15181 cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag
    15241 gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag
    15301 ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggcg
    15361 ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag
    15421 acccggacgg cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc
    15481 gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt
    15541 atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa
    15601 ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt
    15661 tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt
    15721 gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat
    15781 cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt
    15841 attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga
    15901 cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt
    15961 ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag
    16021 gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt
    16081 gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca
    16141 acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt
    16201 attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc
    16261 aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag
    16321 gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa
    16381 ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctgcg
    16441 atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc
    16501 cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact
    16561 gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct
    16621 ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg
    16681 ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc
    16741 atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg
    16801 catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt
    16861 ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc
    16921 ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg
    16981 attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt
    17041 gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt
    17101 tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc
    17161 gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac
    17221 ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc
    17281 ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt
    17341 ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac
    17401 gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca
    17461 cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt
    17521 ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg
    17581 gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct
    17641 gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc
    17701 gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc
    17761 atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg
    17821 gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt
    17881 tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt
    17941 attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt
    18001 actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc
    18061 cttaacaa
//
LOCUS       DAJTRW010000078        18042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRW010000078 DAJTRW010000000
VERSION     DAJTRW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            446..1129
                     /locus_tag="OO326_002894"
     CDS             446..1129
                     /locus_tag="OO326_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5967214.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO326_002895"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO326_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO326_002896"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO326_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5967215.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO326_002897"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO326_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5967216.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO326_002898"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO326_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5967217.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO326_002899"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO326_002899"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5967218.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO326_002900"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO326_002900"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5967219.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO326_002901"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO326_002901"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5967220.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO326_002902"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO326_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5967221.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO326_002903"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO326_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5967222.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO326_002904"
     CDS             complement(13386..13817)
                     /locus_tag="OO326_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5967223.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO326_002905"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO326_002905"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5967224.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO326_002906"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO326_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5967225.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO326_002907"
     CDS             16903..17862
                     /locus_tag="OO326_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5967226.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18042)
                     /locus_tag="OO326_002908"
                     /pseudo
     CDS             complement(17849..18042)
                     /locus_tag="OO326_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4547 a         4661 c         4672 g         4162 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gc
//
LOCUS       DAJTRW010000079        17597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRW010000079 DAJTRW010000000
VERSION     DAJTRW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..624)
                     /gene="chbR"
                     /locus_tag="OO326_002909"
                     /pseudo
     CDS             complement(1..624)
                     /gene="chbR"
                     /locus_tag="OO326_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(632..982)
                     /gene="chbA"
                     /locus_tag="OO326_002910"
     CDS             complement(632..982)
                     /gene="chbA"
                     /locus_tag="OO326_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5967227.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1041..2389)
                     /gene="chbC"
                     /locus_tag="OO326_002911"
                     /pseudo
     CDS             complement(1041..2389)
                     /gene="chbC"
                     /locus_tag="OO326_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2474..2794)
                     /gene="chbB"
                     /locus_tag="OO326_002912"
     CDS             complement(2474..2794)
                     /gene="chbB"
                     /locus_tag="OO326_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5967228.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3093..3431)
                     /gene="osmE"
                     /locus_tag="OO326_002913"
     CDS             complement(3093..3431)
                     /gene="osmE"
                     /locus_tag="OO326_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5967229.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3633..4460
                     /gene="nadE"
                     /locus_tag="OO326_002914"
     CDS             3633..4460
                     /gene="nadE"
                     /locus_tag="OO326_002914"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5967230.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4690..5577
                     /gene="cho"
                     /locus_tag="OO326_002915"
     CDS             4690..5577
                     /gene="cho"
                     /locus_tag="OO326_002915"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5967231.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5537..6112)
                     /gene="ves"
                     /locus_tag="OO326_002916"
     CDS             complement(5537..6112)
                     /gene="ves"
                     /locus_tag="OO326_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5967232.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6315..6800)
                     /gene="spy"
                     /locus_tag="OO326_002917"
     CDS             complement(6315..6800)
                     /gene="spy"
                     /locus_tag="OO326_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5967233.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7130..8098)
                     /gene="astE"
                     /locus_tag="OO326_002918"
     CDS             complement(7130..8098)
                     /gene="astE"
                     /locus_tag="OO326_002918"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5967234.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8091..9424)
                     /gene="astB"
                     /locus_tag="OO326_002919"
                     /pseudo
     CDS             complement(8091..9424)
                     /gene="astB"
                     /locus_tag="OO326_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9421..10899)
                     /gene="astD"
                     /locus_tag="OO326_002920"
     CDS             complement(9421..10899)
                     /gene="astD"
                     /locus_tag="OO326_002920"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5967235.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10896..11930)
                     /gene="astA"
                     /locus_tag="OO326_002921"
     CDS             complement(10896..11930)
                     /gene="astA"
                     /locus_tag="OO326_002921"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5967236.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11927..13147)
                     /gene="astC"
                     /locus_tag="OO326_002922"
                     /pseudo
     CDS             complement(11927..13147)
                     /gene="astC"
                     /locus_tag="OO326_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13593..14399
                     /gene="xthA"
                     /locus_tag="OO326_002923"
     CDS             13593..14399
                     /gene="xthA"
                     /locus_tag="OO326_002923"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5967237.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14566..15276
                     /locus_tag="OO326_002924"
     CDS             14566..15276
                     /locus_tag="OO326_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5967238.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15281..15958
                     /gene="ydjY"
                     /locus_tag="OO326_002925"
     CDS             15281..15958
                     /gene="ydjY"
                     /locus_tag="OO326_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5967239.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15973..16680
                     /locus_tag="OO326_002926"
     CDS             15973..16680
                     /locus_tag="OO326_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5967240.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16680..17228
                     /locus_tag="OO326_002927"
     CDS             16680..17228
                     /locus_tag="OO326_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5967241.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17238..17564
                     /locus_tag="OO326_002928"
                     /pseudo
     CDS             17238..17564
                     /locus_tag="OO326_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4354 a         4677 c         4299 g         4267 t
ORIGIN      
        1 tcgcgtcaaa tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc
       61 actaaactgc tctttatcat gcatcttttc taccgtactt ttcagccact acggtacatc
      121 atcaatcacc tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat
      181 ctcaacaaac tcttccagcc ctgtttcgcg gaaatttaat gaagaaatca ctgtttccac
      241 ataggtgaga aaagcgttat tggtccggta tacctgcgaa gcaacaaagc aataaggcaa
      301 caacggcagg taatgctgct caaaaaagcg tttactgatc ccaacgttca atatgcgcgt
      361 ggcaccaaac tcataaaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc
      421 ccgttccagt aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag
      481 agtaaattca taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat
      541 aaacacatgg aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg
      601 cgcgttaatc actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa
      661 ttaattcagt aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga
      721 ccagcaccag actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct
      781 gtaccagatg cgcttcattc aatgccatcc gtgactgatc catcatggct ttcgctgcgg
      841 caaaatcgcc ctgcttcgcc tgtttcaatg cggcatacgc caggctacgc gcttgtccag
      901 agttgatgat aagccccatc accacttctt ccagctcttc agcttctgtt tgcgtatcag
      961 gaatattatc gagatccatc atacatcgtt cctctctctt cttaccggca agattacccg
     1021 taccggcatc gattaaaatt tcaggggtta gcgatatctt cttcgctctc ttctttatca
     1081 atcgcatttt gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg
     1141 ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct
     1201 cccagaccgg ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg
     1261 tagtacgctg cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac
     1321 atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg
     1381 ccggacggca gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag
     1441 atagccagga tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc
     1501 atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca
     1561 tattgctgat aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt
     1621 gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca
     1681 taggcccagc ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg
     1741 atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa
     1801 agaataataa agccgggaat taatgccgag aaagaacgcg atactgaagc aggtacgctg
     1861 tcgggcagtt taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc
     1921 aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca
     1981 taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca
     2041 gccgccagcg catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc
     2101 attaaagaca ttattcctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca
     2161 ttaagtgttt caatggttga ggcgtcgagg cgaataccta aggaataaaa aacgacccct
     2221 ccccaaagct tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg
     2281 gcattaagcg aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa
     2341 ctgcaaaagg gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc
     2401 cttaaaaacc gcaatttaaa tattacggta ttgatttatg aaataactct ttgacgggaa
     2461 aatttaaaat aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca
     2521 cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg
     2581 gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat
     2641 cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa
     2701 cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg
     2761 cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct
     2821 gtcagccaga cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc
     2881 aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc
     2941 tttgacctta taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc
     3001 aaataattcg cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt
     3061 tccgttgtta ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg
     3121 tattcagcac aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca
     3181 aagtaggttt ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga
     3241 gcatggatca tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg
     3301 ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca
     3361 taagctgtac aacccgccag catggttaat accgccgctg cactcagaat tcctgccata
     3421 ttcttgttca tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg
     3481 ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt
     3541 tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag
     3601 gaagttttgt cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg
     3661 cgctgggcgc aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc
     3721 tgaaaagcta cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg
     3781 gtcaggactc cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg
     3841 aaactcgcaa cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg
     3901 acgaacaaga ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta
     3961 atatcaaggg cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga
     4021 gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg
     4081 cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat
     4141 tcttcactaa atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac
     4201 gccagggtaa acagttactg acggcattag gttgcccgga acacctttat aagaaagccc
     4261 caactgccga tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga
     4321 cctatgacaa tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa
     4381 caatagagaa ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg
     4441 atgatttctg gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg
     4501 ttcacgccgg atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac
     4561 attgcaggag tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat
     4621 caacccgcct cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg
     4681 gagtcaacag tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt
     4741 tatgaatatc ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg
     4801 tatctgtttc atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc
     4861 cgcagtcggg tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc
     4921 cggcgaatta gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga
     4981 ttaatcaaag aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt
     5041 gccctgcaat taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca
     5101 cgagccccca acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag
     5161 agcatcgctg atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc
     5221 ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag
     5281 agccatgagg aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt
     5341 tggccttggc aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat
     5401 attattcaaa actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta
     5461 attcggacac ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt
     5521 tccggtaact atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt
     5581 cactgaacaa cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc
     5641 agcacacgcc ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca
     5701 caaacaccac cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt
     5761 tcgccttaca gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg
     5821 tcagtttcgc ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg
     5881 tatggttaaa gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta
     5941 tcctttccat gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg
     6001 cacgccagta aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg
     6061 cagcgtttcg ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc
     6121 ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg
     6181 gtgatttacg ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa
     6241 ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt
     6301 ttttggctga aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct
     6361 gtcagacgct tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg
     6421 taaattttgt tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt
     6481 tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc
     6541 attgcgcggc gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt
     6601 tcgtggatct gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga
     6661 tgcggaccga acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta
     6721 gtggtgtctg cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac
     6781 agtgcagtta atttacgcat attctatatc cttcctttca gttatttatt acggctttct
     6841 taagtagcgt gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag
     6901 caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga
     6961 aaatttgatg gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa
     7021 gaactatacc ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat
     7081 ttataagaaa gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt
     7141 tttcgagcat taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat
     7201 actctacatc atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt
     7261 tctcaaacgg cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt
     7321 ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac
     7381 tctcaccaga tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt
     7441 tttgcccaaa gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc
     7501 tgacgctgaa atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc
     7561 ccgccgcacc cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata
     7621 caccgaactg cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca
     7681 cagattcttt gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac
     7741 aggtttctcc gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc
     7801 tatggcaata acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc
     7861 gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca
     7921 caggtgccgt ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg
     7981 ttaatggcgt cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt
     8041 ctcgctcggt gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg
     8101 ccccctccct cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca
     8161 tccagtgctt cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaaga
     8221 cgatcgcggt agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc
     8281 atcaccgccg gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg
     8341 cacgccgggc cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta
     8401 atcgggttgt cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga
     8461 cactcctgag gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta
     8521 aacagatagg tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc
     8581 ataaaaccgt tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc
     8641 aaaacagcac ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga
     8701 taacgtccgg gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg
     8761 ccaccgcctc gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat
     8821 tgccttcttc tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga
     8881 gacgattgtg gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat
     8941 ggacgctaaa tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg
     9001 cttccagcga acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat
     9061 ccagcgtatc ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg
     9121 aactgacgct ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat
     9181 cgctaccact gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa
     9241 tcacggcctg ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg
     9301 ccgccagtcg cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg
     9361 acaggcccgc gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt
     9421 tcatcgcacc acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa
     9481 cgagtccgac tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct
     9541 ggggcgatgg ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt
     9601 aagcggtttg ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt
     9661 ttcccgctcg ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg
     9721 aatcgcctca tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc
     9781 atccggcaca ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc
     9841 tgcttgtaat aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca
     9901 ggcagtaacc acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg
     9961 ttcgtcatcc cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa
    10021 cgcatcgccc tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc
    10081 ggctgtgaca aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc
    10141 atcgataatt agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc
    10201 ggagagctgg cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc
    10261 gagatcctcc agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag
    10321 cacgcccggc ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg
    10381 tgtcagttcg ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg
    10441 tccgttcggc aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg
    10501 gcgatgtcgc aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac
    10561 gtgatacgcc ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc
    10621 ttcccagcgc ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct
    10681 ttccagcaat ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc
    10741 ccagcgcgga aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc
    10801 atcattgcct tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc
    10861 ctggcccgtt atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac
    10921 aggcgcacca gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg
    10981 gttaaaatca aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga
    11041 taattttcat tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc
    11101 acttccacca gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt
    11161 ggcccgccgt cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc
    11221 gcgcgggcag gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat
    11281 aaaaagtggg tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg
    11341 gtgccgcaga gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt
    11401 tgccagaacg gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg
    11461 tcattaaact tgtcgcgaaa agccgccata aacataaagc gcgatttcga cagcaaatag
    11521 ccgttgccct ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg
    11581 gtgtgatcgt tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag
    11641 gcgtgaacca acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc
    11701 tcaatagcgc aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag
    11761 ccctgctcgc ttttgggcag ttcaccttgc caggttttga ttgccctttc gatacgcgcc
    11821 gaaagcgtgg cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc
    11881 tgcatcagcg ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc
    11941 ggctaacaaa gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt
    12001 cgctgacatt gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca
    12061 cgcctgcttt cgccgcttcc agagagatct gtttcgcttg cccggcgtaa tcggcattca
    12121 gtacacagcc aatcaacaaa cctaagccgc gaatttcact gaacaaacca tagcggtgat
    12181 taatagtatt aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct
    12241 ctggtgtgtt gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc
    12301 cataggtggt gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca
    12361 acgcaccgac cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca
    12421 cgccgtagtg catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat
    12481 caaaaatcag caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt
    12541 tgctggctgg caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg
    12601 cgtcgtcaat cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg
    12661 ccggcagtgg cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca
    12721 gcgtgcgacc atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc
    12781 ggtcgtgagc gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt
    12841 tacaaaagaa gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta
    12901 ccggctcgtt ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg
    12961 cttcacgcag ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt
    13021 cgatatactc tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa
    13081 agggtgccgg agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct
    13141 gagacatagc gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt
    13201 ttgcgctgta aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg
    13261 cggaggctaa ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata
    13321 aagtgaatac gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat gttatgcaga
    13381 agtaaaatat aattctagaa ttgtgatcat tgacgaaatt tactggaaat tactgcgcca
    13441 ttctgacgca gcgcgcacca aaagcgggca ttttttgcgc catcgttgac atcattaaca
    13501 accatcgatc aaatcactta acaacaggcg gtaagcaacg cgaaattctg ctaccatcca
    13561 cgcactcttt atctgaataa atggcagcga ctatgaaatt tgtctctttt aatatcaacg
    13621 gcctgcgcgc cagacctcac cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga
    13681 ttggcctgca ggagacaaaa gttcatgacg atatgtttcc gctcgaagag gtggcgaagc
    13741 tcggctacaa cgtgttttat cacgggcaga aaggccatta tggcgtggcg ctgctgacca
    13801 aagagacgcc gattgccgtg cgtcgcggct ttcccggtga cgacgaagag gcgcagcggc
    13861 ggattattat ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc aacggttact
    13921 tcccgcaggg tgaaagccgc gaccatccga taaaattccc ggcaaaagcg cagttttatc
    13981 agaatctgca aaactacctg gaaaccgaac tcaaacgtga taatccggta ctgattatgg
    14041 gcgatatgaa tatcagccct acagatctgg atatcggcat tggcgaagaa aaccgtaagc
    14101 gctggctgcg taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg atggacaggc
    14161 tgatgagctg gggggtggtc gataccttcc gccatgctaa tccgcaaaca gcagatcgtt
    14221 tctcatggtt tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc
    14281 tgctgctcgc cagccaaccg ctggcagaat gttgcgtaga aaccggcatc gactatgaaa
    14341 ttcgcagcat ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc cgccgctaat
    14401 ttagcagctc tcctggctca aactgggtca ggagaattaa ccttgagaaa aatcaacaaa
    14461 ctgtcagtaa tgatttgttg cctgccgtcc tttgttatac cgtcactgcg tttttagttg
    14521 tctgaccact tctctattat caagtttgat ataggaaact ccacgatgaa cgctgagcgt
    14581 aactttctgt ttgcctgtct tatttttgcg ctggtcattt acgctatcca cgctttcggt
    14641 ttattcgatc tgctcaccga tttaccccac ttacagacac tcattcgcca gagcggattg
    14701 ttcggttata gcctctatat tctgttattc atcaatgccc ccctctttct gttaccagga
    14761 agcatactgg tgatcgctgg aggaatagtt tttggcccgc tcttagggac acaactctca
    14821 ttaattgccg caacgctggc ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt
    14881 gatttactgc tgaaatacgt gggccatagc cataccttcc aggccattga aaaaggcatt
    14941 gcgcgtaacg gtattgattt tcttattctg acccgcttaa tcccgttgtt tccttacaat
    15001 attcaaaatt acgcttacgg attcaccacc atcgccttct ggccttatac ccttatttcg
    15061 gcactcacga ccctgcccgg tattgttatt tataccgtga tggcaagcga tctcgccaat
    15121 gaaggcatta cgctgcgctt tattttacaa ctctgtctgg cgggcctggc gctgtttatt
    15181 ctcatccagc tcgcaaaact ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc
    15241 cgcagcccac ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac attattcagt
    15301 gtcatggaaa aaaggactgg ctgcactctg cttactggct gttgccgggc ttagcggctg
    15361 cgatcaaaaa gagaatgcgg cggcaaaagt ggaatacgac ggactttcga acagccaacc
    15421 gctgcgcgtc gatgccaata accatacggt aaccatgctg gtgcaaatta atggtcgttt
    15481 cctcaccgac gacactcgtc acggtattgt gtttaaagat ggctccaacg gacataaatc
    15541 gctgtttatg gcttatgcgc ccccgaaagc attttatgaa gccctgaaag aggcaggtgg
    15601 tacgccgggc gaaaacatga cgatggataa taaagaaacg actcatgtca caggcagcaa
    15661 actggatatt tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat
    15721 tgttgacagt aatggcaaga aactggacat gcgctttggc ggtaatttaa cggcagcaga
    15781 agagaagaaa acgggttgcc tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa
    15841 tgcaacatac acttatggtg cggtggaaaa acacggcgaa gtgaaattca aaggcaatgc
    15901 ctcagttctc ccggcggata acacgctggc aacggttacc tttaaaatca ccgaataaag
    15961 ccaagataaa ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg tataaccctc
    16021 atcatcctgt tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt
    16081 atcaatcgca gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt
    16141 cagtcttacg gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt
    16201 gctgcaccgc tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc
    16261 tggggcggct tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg cttttttatc
    16321 gccagagtga tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt
    16381 atggacggct ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg gttattgcct
    16441 tttgtccctt tcgatccaat cagctatgct gccggtctga cttcaatacg ttttcgctcg
    16501 ttttttatcg ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcaggc
    16561 agcatgttaa caggcggtac tttctggttt gtcaccgaac tgtttattct gtttgccctg
    16621 accgtggtga tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgactga
    16681 tgggtttacc gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc
    16741 gtcgccatta cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc ccgtttatct
    16801 tttatttggt gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg
    16861 tagtgcgatc gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg
    16921 atatcaccag tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg
    16981 ctgactggcg gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtacgccg
    17041 aagccgcaag cgtcacaccg ccaacagtcg atgatgccct gcgtacccga ctggctgcgc
    17101 attttgacgc tcaggcgctc accgaactga cggcattgat cggcctgcaa aacctgtcgg
    17161 ctcgttttaa ttctgccatg gacattcccg ctcaggggct gtgccgtatt ccggaaaaac
    17221 gttcttaagg agggaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt
    17281 ctttggcaac acatgccagt gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg
    17341 tctggtttaa cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg
    17401 gcgagatgaa aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag
    17461 acgcggtgaa gcgcattcag accgaagctg ctgccggtcg taaaacgggc ggctcggtgg
    17521 atctgctctg ggtgaacggc gaaaacttcc gcaccttaaa agagtaatgc cagtcagtta
    17581 agcaactgac tggcatt
//
LOCUS       DAJTRW010000080        17292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRW010000080 DAJTRW010000000
VERSION     DAJTRW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO326_002929"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO326_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5967242.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO326_002930"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO326_002930"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5967243.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO326_002931"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO326_002931"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5967244.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO326_002932"
     CDS             complement(2205..2348)
                     /locus_tag="OO326_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5967245.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO326_002933"
     CDS             complement(3019..3306)
                     /locus_tag="OO326_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5967246.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO326_002934"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO326_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5967247.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO326_002935"
     CDS             3683..3922
                     /locus_tag="OO326_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967248.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO326_002936"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO326_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5967249.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO326_002937"
     CDS             5033..6406
                     /locus_tag="OO326_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967250.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO326_002938"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO326_002938"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5967251.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO326_002939"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO326_002939"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5967252.1"
                     /translation="MNMFFRLTALAGLLAIAGQTVAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO326_002940"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO326_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5967253.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO326_002941"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO326_002941"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5967254.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO326_002942"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO326_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5967255.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO326_002943"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO326_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO326_002944"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO326_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO326_002945"
     CDS             16524..16760
                     /locus_tag="OO326_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5967256.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO326_002946"
     CDS             16865..17056
                     /locus_tag="OO326_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5967257.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17292)
                     /locus_tag="OO326_002947"
     CDS             complement(17081..>17292)
                     /locus_tag="OO326_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5967258.1"
                     /translation="RYCQYTSVYEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWF
                     GHTPLRHRVDIGNLHYIDTGAVFGG"
BASE COUNT         4195 a         4359 c         4170 g         4568 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gacggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcataaact gaggtgtact
    17281 ggcaatagcg ga
//
LOCUS       DAJTRW010000081        16911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRW010000081 DAJTRW010000000
VERSION     DAJTRW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            182..1315
                     /gene="fepE"
                     /locus_tag="OO326_002948"
                     /pseudo
     CDS             182..1315
                     /gene="fepE"
                     /locus_tag="OO326_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1312..2127)
                     /gene="fepC"
                     /locus_tag="OO326_002949"
     CDS             complement(1312..2127)
                     /gene="fepC"
                     /locus_tag="OO326_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5967259.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2124..3116)
                     /gene="fepG"
                     /locus_tag="OO326_002950"
     CDS             complement(2124..3116)
                     /gene="fepG"
                     /locus_tag="OO326_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5967260.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3113..4117)
                     /gene="fepD"
                     /locus_tag="OO326_002951"
     CDS             complement(3113..4117)
                     /gene="fepD"
                     /locus_tag="OO326_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5967261.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4228..5460
                     /gene="entS"
                     /locus_tag="OO326_002952"
     CDS             4228..5460
                     /gene="entS"
                     /locus_tag="OO326_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5967262.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5464..6420)
                     /gene="fepB"
                     /locus_tag="OO326_002953"
     CDS             complement(5464..6420)
                     /gene="fepB"
                     /locus_tag="OO326_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5967263.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6712..7887
                     /gene="entC"
                     /locus_tag="OO326_002954"
                     /pseudo
     CDS             6712..7887
                     /gene="entC"
                     /locus_tag="OO326_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7897..9507
                     /gene="entE"
                     /locus_tag="OO326_002955"
     CDS             7897..9507
                     /gene="entE"
                     /locus_tag="OO326_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5967264.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9521..10378
                     /gene="entB"
                     /locus_tag="OO326_002956"
     CDS             9521..10378
                     /gene="entB"
                     /locus_tag="OO326_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5967265.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10378..11124
                     /gene="entA"
                     /locus_tag="OO326_002957"
     CDS             10378..11124
                     /gene="entA"
                     /locus_tag="OO326_002957"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5967266.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11127..11540
                     /gene="entH"
                     /locus_tag="OO326_002958"
     CDS             11127..11540
                     /gene="entH"
                     /locus_tag="OO326_002958"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5967267.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11721..13826
                     /gene="cstA"
                     /locus_tag="OO326_002959"
     CDS             11721..13826
                     /gene="cstA"
                     /locus_tag="OO326_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5967268.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            14008..14205
                     /locus_tag="OO326_002960"
     CDS             14008..14205
                     /locus_tag="OO326_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5967269.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14215..15303)
                     /gene="hcxA"
                     /locus_tag="OO326_002961"
     CDS             complement(14215..15303)
                     /gene="hcxA"
                     /locus_tag="OO326_002961"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5967270.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15412..16572
                     /gene="ybdL"
                     /locus_tag="OO326_002962"
     CDS             15412..16572
                     /gene="ybdL"
                     /locus_tag="OO326_002962"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5967271.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16573..>16911)
                     /locus_tag="OO326_002963"
     CDS             complement(16573..>16911)
                     /locus_tag="OO326_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967272.1"
                     /translation="AMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAAT
                     IRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFAD
                     RQYSRAWTVK"
BASE COUNT         3746 a         4412 c         4823 g         3930 t
ORIGIN      
        1 ataattatgg gttttttatt tgtttgattt ataggtttga tgaatatttc tcttaaatag
       61 agtgaatgtt gcattcagtg gaaggaattt acaaacttca gcttcggata attctcacca
      121 actggtaaag tgagcgccgt ggagattttc ccctattgga ctcattttca ggttataccc
      181 catgtcatca ttgaatatta aacagggaag tgacgctcat ttttctgagt atcctctggc
      241 gtcgcccagt aataatgaaa ttgatttact taatctaatc gaagttttat ggcgggccaa
      301 aaaaacggtc atggcggtcg tttttgcatt tgcctgcgca ggtttactga tctctttcat
      361 cctgccgcaa aaatggacca gctcagccgt tattactccc gccgaagcca ttcagtggca
      421 ggacctggag aaaaccttta ccaaactccg cgttcttgat ctcgatgtta atatcgaccg
      481 tggcggtgca tttaacctgt ttatcaagaa gtttcagtct gttagcttgc tggaagagta
      541 cctgcgttca tcaccttatg tgatggacta attaaaagag gcgaaaatcg atgaactgga
      601 tttgcatcgc gctattgttg cattgagcga aaaaatgaaa gcggttgatg acaatgctag
      661 taagaaaaaa gatgaaccgt cactgtatac atcctggacg ctaagtttta ccgcgccaac
      721 cagtaaagag gcgcagaccg ttttgagcgg gtatatcgat tatatctctg cgttggtggt
      781 gaaagagtcg atagaaaacg tccgtaataa actggagatc aaaacccagt ttgaaaaaga
      841 aaaactggct caggatcgca ttaaaacgaa aaatcaactt gatgcaaaca ttcagcgcct
      901 caattattca ctcgacattg ccaacgcggc aggaattaaa aagcccgtat acagtaatgg
      961 tcaggccgtt aaagatgacc ccgatttctc tatttccctc ggcgcagacg gtattgaacg
     1021 caaactggaa attgaaaaag cggttactga cgttgcggaa ctgaacggtg aattacgtaa
     1081 tcggcagtat cttgtcgagc aattaacaaa agcaaatgtc aacgatgtga attttacgcc
     1141 gtttaaatat cagttaagcc cgtcattgcc agtgaaaaaa gacggtccgg gtaaggcgat
     1201 tattgtgatc ctttccgcgc tgatcggcgg gatggtggct tgtggtagcg tgttattgcg
     1261 ctatgcgatg gcatccagaa aacaggatgc catgatggca gaccacttag tttaactatt
     1321 tgcggttgaa ggtgccgttc gtccgagcgg caccacaagc ggcattccgg ctaccggatc
     1381 gtcaatgatc atgcatcgca gaccataaat gcgctcaatc agttcagcag tgacaatctc
     1441 cttcggcgct ccctgagcaa caattttccc ttcccgcaat gcaatcaaat ggctggcgta
     1501 acgacaggcc tgattaagat cgtgcagcac cgccgccagg gtatagcctt tctcgcggtt
     1561 cagttcgctt aacagttcca gtagatcaat ctgatgactg atatccagcc aggtcgtggg
     1621 ttcgtcgagc agcataattg ccgtttcctg ggccagcacc atcgcgatcc acgctcgctg
     1681 gcgttgtccg ccagaaaggg tatccacgct ttgatctgcc agatgagtta ttcccgtggc
     1741 ctgcatcgct ttcgttaccg cttcttcatc ctctttgcgc cagcgggtaa acagcggttg
     1801 atgcggataa cgtccacgcg ccaccagctc ctgcacggtg atatcgcccg gcgtggtagc
     1861 attttgcgcc aacagaccaa tccggcgtgc aacttcttta ctggcgtaat gttgaatgtg
     1921 ctcgccatcc agccagacat gcccgtgagc aggcgtcatc aggcggctta aggtacgcag
     1981 taacgtggat ttaccacagc catttggtcc gataattgcc gtgaagtgac catcaggaat
     2041 ttctacagtc agattttccg caacggtata tttgccatat cccagggtta actgttcgcc
     2101 gcgcaaacgg gctactgatt cggtcatttt ttgcgagact cctgaattaa caagacgata
     2161 aggtaaatac cgccgaggct gacggtaacg acgccaaccg gaagttgata cggcataaac
     2221 agttgttggg cgcagagaac ggccgccagc agtaacagcg ctccgcacag cgccgcctgg
     2281 gtcagccccc agcgagcggt gccgctaatg cgtcgggcaa tgtgcggcgc gactaaagca
     2341 ataaaggaaa tcggcccggc aagcgccgtt gccgcagcgg taagcaccac tgcaaccagc
     2401 atcattaaca gacgcgaacg ctcgacgctg acgcccagcg cgcaggcggt atcatcgccc
     2461 atctccagca agcgcattcg ccgcaccagc aaggtggcgg caatgagcat caatataatg
     2521 atgggtgcgg aaggcgaggt ttttgcccac gtcaggccgt tgagcgatcc ggcattccac
     2581 aaacctgctg ttagcgccgt ttctaaagac gctttcagca acagccaggt attaaaggcc
     2641 accagcatgg cgcgaacgcc gataccgata ataatcaacc gaaaggtgtc gataccgtta
     2701 cgccaggcga gcagccagac caacagcgaa gtgataatgc cgcccaccat tgctgccagc
     2761 gcgatagccg tcagatcctg accaaacagc accatcgcca ccagcacgcc gctccacgcc
     2821 ccggtgttaa agcccattac gtcagggctg ccgagcgggt tacgcatcag cgactgaaaa
     2881 atcgcgccgc tgacgcccag cgccgcgcca atcaacagcg ccatcagcac gcgtggtaaa
     2941 cgccattcgg tgaccaccat cgtcatactg cgcggcgcat cgcccatcaa cgcggcgaat
     3001 acctgcgaga tttccagcgt gacggcaccg ctgcgtaatc cccagatacc tgcaaccaca
     3061 caggcggaaa tcagcagcaa acaggtgatg agtaatcggc gagagacgta aatcatgcac
     3121 cacctcgcgt tttacgtcgc acgaggaaga tcagcaccgg tgcaccaata aacgcactga
     3181 ccacagaaac gcgcagttcg ccgggcacaa tcacccgccc gatgatatcg gcaaacagca
     3241 gcagggcagg ggtagcaagt agcgtgacgg gcagcgacca gcgatgatcg gcacctacca
     3301 gccagcgcgc catgtgcggc atcatcaggc cgataaaggc aatcgggcca actaccgccg
     3361 tcgcactacc acaaagcacg gtaatcgcca gcagaccaat caactgtgtg cgcgccacgc
     3421 gactgcccag cgccgtcgcg gtgtcactgc cgaggctcaa actgttcagc gcgcgactca
     3481 gtaatagcgc agttgctccg gcgatcagaa ccgggatcag caccactttt aaggtatgta
     3541 gattgcgaat atccagcgaa ccggcttgcc agaaacgcag ttgatcgtag acgtcaggat
     3601 taagcagggc gatgccgctg gtcagtcctt ccagcaccgc cgccagcgcc acgcccgcca
     3661 gggttaaacg caccggactt aactgccctc caccctgact gccggtaaag gcaacaatca
     3721 atgaggccac cagcgccccg gcgaaggcca tcgccagttg ttcctgtgcg gatgagtaac
     3781 caaacagcgc cgcacccagc acaatggcaa agctggctcc ggcgttcacg ccaagcaagc
     3841 cggggtcggc aagtgggttt cgggtgaggg tttgcattaa cgccccggca aggccaagcg
     3901 cgccgcctgc cagtaaaccg gcaagggtac gcggcagccg cgcgtcgagc acgatggtgc
     3961 agtcggcgct ctggcaggtg ccggagaagg cctccagcac tacggaagcg gggagtgatt
     4021 ttgccccaat gagcaggctt aatgccgtcg cgataatcag taataacagc aatccgggca
     4081 cggcaatggc gcgtgtcacg gcaacagaac cagatatacg aacttccatg ataatgaaat
     4141 taattatcgt tatcgatctt atttggatat gttaacatgt gcagcctaag aataggtatt
     4201 taaaatattt gatggcaagg cattgtaatg aataaacaat cctggctgct taacctcagc
     4261 ctgttgaaaa cgcacccggc gtttcgcgca gtattcctcg ctcgttttat ctcaattgtg
     4321 tctctgggtt tgctcggcgt cgcggtgccg gtgcagatcc agatgatgac ccattctacc
     4381 tggcaggtgg ggctttcggt gacgctgacc ggcggcgcga tgtttgttgg cctgatggtt
     4441 ggcggtgtgc tggcggatcg ctatgaacgc aaaaaagtga ttttgctggc gcgcggcacc
     4501 tgtggcattg gcttcattgg actgtgcctt aatgcactgc tgccggagcc gtcattgctg
     4561 gcaatctatt tacttggttt atgggatggt tttttcgcat cacttggtgt tacggcgcta
     4621 ctggcggcaa catctgcact ggtagggcgt gaaaacttaa tgcaggccgg ggcgatcacc
     4681 atgttgaccg tgcgcctggg gtcggtgatt tcgcccatga ttggcggttt attgctggcg
     4741 accggcggcg tagcctggaa ctacgggctg gcggcggcgg gcacgtttat taccttgtta
     4801 ccgttattaa gccttccgga gttgccaccg ccaccgcagc cgcttgagca tccgttgaaa
     4861 tcattactgg caggatttcg ttttctgctt gccagcccgc tgctgggtgg tttattgacg
     4921 atggcgagcg cggtgctggt actgtatccg gcgctggctg acaactggca gatgtcagcg
     4981 gcacagattg gttttctcta cgcggcgatc ccgctcggcg cggctattgg tgcgttaacc
     5041 agcgggaagc tggcacatag tgcgcgacca gggttattga tgctgctctc cacgctggga
     5101 tcgttcctcg ccattggtct gtttggcctg atgccgatgt ggattttagg cgtggtttgt
     5161 ctggcgctgt tcggctggct gagcgcggtt agttcgttgc tgcaatatac aatgctgcaa
     5221 acgcaaaccc cggaagcgat gttagggcgg attaacggtt tgtggacggc gcagaacgtg
     5281 acgggcgatg ccataggcgc ggcgctgctg ggtggtttgg gcgcgatgat gacaccggtt
     5341 gcttccgcaa gcgcgagcgg ttttggtttg ttgattatcg gcgtgttgtt attgctggtg
     5401 ctggtggagt tgcgacgttt tcgccagacg ccgccgcagg tgacagcgtc cgacagttaa
     5461 tgcttaaaac agcgccttaa gccgttccag cacttgcatg gcgctgtagt aatccaggcg
     5521 gaacgtctcg gttcccagcg cataaacctg cttgttttgt accgcaggca ggtgcgcgag
     5581 cagtggattg gcataaatcg catcggcatc tttctgatca ccagcgaaca ggaatagcga
     5641 ttcaccgttt aaccccgcag ccagattttc cccaccaagc tgaatgatgt catggcgttt
     5701 gccctggctt tggctggcgt ttaaccctgc gggcaatttc gccagcgtaa agccgagttg
     5761 ttccagcatc tgcccttgtg ctgattccgg cgtccagaga ttggcactgt gtgcagcggc
     5821 ggtatagaca atggcagtga ccggctgcgg cggtaatttg atttgctctt tcgccgctgc
     5881 cagttgctta tcaaactgtg caatccgctc tgccgcttgt ttctcatgcc cggtaatttc
     5941 gccaagctgc gttaacagcg actgccagct tttgtcgtcg taattgatga ttaatgtcgg
     6001 ggcgatggtg gaaagctgat catacagcgc cagcgccgaa tccccgccgg ttgcgctaat
     6061 taaaatcaga tccggcattt gcgcggcaac ggcttcggcg ctcggttcgc cgatatagag
     6121 ccgttgcagt ttgcgttctt tcgccacctt gctccactgg cgtaaaaagc cctggtcatc
     6181 tgcgacgcgg ttattcggcg tggtcgcgcc gctggcgatc accggagcat caatcgccag
     6241 cagtgatccg gtcagggtga cgctggtgga aacaatacgc tgcggctggc tttccagggt
     6301 atgagtgccc cggctgtcag taatctgacg cggccagtcg gcggcctgaa ctacggctat
     6361 tcctgaaagc aaaagtcctg ttaatagaag ggcgttgcgg tagagcgggg cgagtctcac
     6421 aagtcagctt cctgttatta ataaggttaa gggcgtaatg acaaattcga caaagcgcac
     6481 aatccgtccc tttggggaga gggggcaaaa cggcgcagga catcacattg cgcttatgcg
     6541 aattcatcaa taatgcttct cattttcatt gtaaccacaa ccagatgcaa ccccgagttg
     6601 cagattgcgt tacctcaaga gttgacatag tgcgcgtttg cttttaggtt agcggccgaa
     6661 aatataagtg ataatcatta ttaaagcctt tatcattttg tggaggatga tatggatacg
     6721 tcactggctg aggaagtaca gcagaccatg gcaacacttg cgcccaatcg ctttttcttt
     6781 atgtcgccgt accgcagttt tacgacgtca ggatgtttcg cccgtttcga tgaaccggct
     6841 gtgaacgggg attcgcccga cagtcccttc cagcaaaaac tcgccgcgct gtttgccgat
     6901 gccaaagcgc agggcatcaa aaatccggtg atggtcgggg cgattccctt cgatccacgt
     6961 cagccttcgt cgctgtatat tcccgaatcc tagcagtcgt tctcccgtca ggaaaaacag
     7021 acctcagccc gctgttttac ccgcagccag tcgctgaacg tggtggaacg ctaggcaatt
     7081 cctgaacaaa ccacgtttga acagatgggt gcccgcgccg ccgcacttac cgccacgccg
     7141 caggtcgaca aagtggtgtt gtcacgattg attgatatca ccactgacgc cgccattgat
     7201 agtggcgtat tgctggaacg gttgattgcg caaaacccgg ttagttacaa cttccatgtc
     7261 ccgctggctg atggtggcgt cctgctgggg gccagcccgg aactgctgct acgtaaagac
     7321 ggcgagcgtt ttagctccat tccgttagcc ggttccgcgc gtcgtcagcc ggatgaagtg
     7381 ctcgatcgcg aagcgggtaa tcgtctgctg gcgtcagaaa aagatcgcca tgaacatgaa
     7441 ctggggactc aggcgatgaa agaggtactg cgcgaacgca gtagtgagtt acacgttccc
     7501 tcctctccac aattgattac cacgccgacg ctgtggcatc tcgcaactcc ctttgaaggt
     7561 aaagcgaatt cgcaagaaaa cgcactgact ctggcctgtc tgctgcatcc gacccccgcg
     7621 ctgagcggtt tcccgcatca ggccgcgacc caggttattg ctgaactgga gccgttcgac
     7681 cgcgaactgt ttggcggcat tgtgggttgg tgtgacagcg aaggtaacgg cgaatgggtg
     7741 gtgaccatcc gctgcgcgaa gctgcgggaa aatcaggtgc gtctgtttgc cggagcgggg
     7801 attgtgcctg cgtcgtcacc gttgggtgag tggcgcgaaa caggcgtcaa actttctacc
     7861 atgttgaacg tttttggatt gcattaagga gcgaggatga gcattccatt cacccgctgg
     7921 ccggaagagt ttgcccgtcg ctatcgggaa aaaggctact ggcaggattt gccactgacc
     7981 gacattctga ctcgccacgc tgcgagtgac agcatcgcgg ttatcgacgg cgagcggcag
     8041 ttgagttatc gggagctgaa tcaggcggcg gataacctcg cctgtagttt gcgccgtcag
     8101 ggcattaaac ctggtgaaac cgcgctggta caactgggta acgtcgctga actttacatt
     8161 acctttttcg cgctgctgaa actgggcgtc gcgccggtgc tggcgctgtt cagccatcag
     8221 cgtagtgaac tgaacgccta cgccagccag attgaaccgg cgttactgat tgccgatcgc
     8281 cagcatgcgc tatttagcgg ggatgatttc ctcaacacat ttgttgcaga gcattcttcc
     8341 attcgcgtgg tgcaactgca caacgacagc ggtgagcata acttgcagga tgcgattaac
     8401 catccggcag agggttttac tgccacgcca tcacctgctg atgaagtggc ctatttccag
     8461 ctttccggcg gcaccaccgg cacaccgaaa ctgatcccgc gcactcataa cgactattac
     8521 tacagcgtgc gtcgtagcgc cgagatttgt cagttcacac aacagacgcg ctacctgtgc
     8581 tcgatcccgg cggctcataa ctacactatg agttcgccag gatcgctggg cgtctttctt
     8641 gccggaggaa cggttgttct ggcggccgat cccagcgcca cgctttgctt cccgttgatt
     8701 gcaaaacatc aggttaacgt taccgcgctg gtgccgccgg cagtcagcct gtggttgcag
     8761 gcgctggctg aaggcgaaag ccgggcgcag cttgcctcgc tgaaactgtt acaggtcggc
     8821 ggcgcacgtc tttctgccac gcttgcagcg cgtattcccg ctgagattgg ctgccagttg
     8881 cagcaggtgt ttggcatggc ggaagggctg gtgaactaca cccgtcttga tgatagcgcg
     8941 gagaaaatta tccataccca gggttaccca atgtgtccgg acgatgaagt gtgggttgcc
     9001 gatgccgaag gaaatccact gccgcaaggg gaagttggac gcctgatgac gcgcgggccg
     9061 tacaccttcc gtggctatta caaaagtccg cagcacaatg ccagtgcctt tgatgccaac
     9121 ggtttttact gttccggcga tctgatctct attgatccag agggttacat caccgtgcag
     9181 gggcgcgaga aagatcagat caaccgtggc ggcgagaaga tcgctgccga agatatcgaa
     9241 aacctgctgc tgagccatcc ggcagtgatc tatgccgcac tggtgagcat ggaaaatgag
     9301 ctgatgggcg aaaaaagctg tgcttatctg gtggtaaaag agccgctgcg cgcggtgcag
     9361 gtgcgtcgtt tcctgcgtga acagggtatt gccgaattta aattaccgga tcgcgtggag
     9421 tgtgtggatt cacttccgct gacggcggtc gggaaagtcg ataaaaaaca attacgtcag
     9481 tggctggcgt cacgcgcatc agcctgaagg agagaacacg atggctattc caaaattaca
     9541 ggcttacgca ctgccggagt ctcacgatat tccgcagaat aaagttgact gggcctttga
     9601 accgcaacgt gccgcgttgt taatccatga tatgcaggac tattttgtca gcttctgggg
     9661 cgagaactgc ccgatgatgg agcaggtgat cgcgaatatt gctgcgctgc gcgactactg
     9721 caaacagcac aatatcccgg tttattacac cgcccagccg aaagagcaga gcgatgaaga
     9781 tcgggcgctg ttgaatgata tgtgggggcc gggcctgacc cgctcgccgg aacagcaaaa
     9841 ggtggtggat cgcctgacgc cagatgccga cgacacggtg ctggtgaagt ggcgctacag
     9901 cgcgtttcat cgttctccgc tggagcaaat gctcaaagag agtggacgta accagctgat
     9961 tattacagga gtatatgccc acattggctg tatgaccacc gcaaccgacg catttatgcg
    10021 cgatattaaa ccgtttatgg tggcggatgc gctggctgat ttcagccgtg acgagcattt
    10081 gatgtcgctg aaatatgtgg ccggacgttc tggccgggtg gtgatgaccg aagaattact
    10141 gccagcacct atccccgcca gcaaagcggc gctgcgtgag gtgatcctgc cgttgctgga
    10201 cgagtccgat gaaccgttcg atgacgacaa cctgatcgac tacggtctgg attcggtgcg
    10261 catgatggcg ctggcggcgc gctggcgcaa agtgcatggt gatatcgact ttgtcatgct
    10321 ggcgaaaaac ccgaccatcg acgcctggtg gaagctactc tcccgcgagg tgaaataatg
    10381 gatttcagcg gtaaaaatgt ctgggtaacc ggcgcaggta aaggtatcgg ctacgccacg
    10441 gcgctgacgt ttgttgaggc gggagcgaaa gttacaggtt ttgatcaagc gttcactcag
    10501 gagcaatatc cctttgcgac cgaagtaatg gatgttgccg acgccgggca ggtggcgcag
    10561 gtatgtcagc gactgttagc tgaaacggag cgactggacg tgctgatcaa tgcggcggga
    10621 attttgcgca tgggcgcgac cgatcagctc agtaaagagg actggcagca gacttttgcg
    10681 gttaacgtcg gcggtgcgtt taacctgttc cagcaaacca tgaaccagtt tcgccgtcag
    10741 cggggcgggg cgattgtcac tgtggcgtcc gacgccgcac acacggcgcg tattggtatg
    10801 agtgcttatg gcgcatcgaa agcggcgctg aaaagcctgg cgttgagcgt cgggctggaa
    10861 ctggcgggta gcggcgtgcg ctgtaatgtg gtttcgcctg gctccaccga caccgatatg
    10921 caacgcacgc tgtgggtgag cgatgacgcc gaagaacagc gtattcgcgg ctttggcgag
    10981 cagtttaaac tcggcattcc gctggggaaa atcgcccgtc cacaagagat cgccaacacg
    11041 attttgttcc tcgcctctga cctcgccagc catatcaccc tacaggatat tgtggtcgat
    11101 ggcggctcaa cgctgggggc ataagcatga tctggaaacg ccatttaacg ctcgacgaac
    11161 tgaacgccac cagcgataac acaatggtgg cgcatctggg aattgtgtat acccttctgg
    11221 gagatgatgt gctggaagcc gaaatgccgg ttgatacccg tactcatcag ccgtttggcc
    11281 tgctgcatgg cggcgcgtcg gcggcgctgg ctgaaacgct gggatcgatg gccggattta
    11341 tgatgacccg tgacgggcag tgtgtggtgg gcacggaact taacgccacc catcatcgcc
    11401 cggtgtctga aggcaaggta cgcggcgtct gccagccgct gcatcttggg aggcaaaatc
    11461 agagttggga aatcgtcgtt ttcgatgaac aggggcggcg ttgctgcact tgtcggctgg
    11521 gtacggcagt tttgggatga gcccgacaga attaaatgag attgcaggaa aactcggtta
    11581 acggagtgat cgagttaaca ttgttaagtt aaatattggt ttcaactccg atttacatgg
    11641 ttaccgtgtt gttaaatcgt acaaagatgt tatagaaaca aaatgtaaca tctctatgga
    11701 cacgcacacg gataacaact atgaacaaat cagggaaata cctcgtctgg acagtgctct
    11761 ctgtaatggg agcatttgct ctgggataca ttgctttaaa tcgtggggaa cagatcaacg
    11821 cgctgtggat tgtggtggcg tcggtctgta tctatctgat cgcttaccgt ttttatgggc
    11881 tgtatatcgc caaaaatgtg ctggcggttg acccgacgcg tatgacgcca gcggtgcgcc
    11941 ataacgacgg gctggactat gtgccgacgg acaagaaagt gctgttcggt caccattttg
    12001 cggccattgc cggagcaggg ccgctggtgg ggccggtgct ggcagcgcaa atgggctacc
    12061 tgccggggat gatctggctg ctcgccgggg tggttctcgc cggtgcggtg caggatttca
    12121 tggtgctgtt tgtttctacg cgccgtgacg gtcgctcgct gggtgagctg gtcaaagaag
    12181 agatggggcc aactgccggg gtgattgcgc tggtagcctg ctttatgatc atggtcatta
    12241 tccttgcagt actggcgatg atcgtggtga aagccctgac tcatagcccg tggggaacgt
    12301 ataccgttgc gttcaccatt ccgctggcga tttttatggg aatctacctg cgctatctgc
    12361 gtccgggccg cattggcgaa gtgtcagtga ttggcctggt gttcctgatt ttcgccatta
    12421 tctctggcgg ctgggtggca gaaagtccga cctgggcacc gtactttgac tttaccggcg
    12481 tgcagctgac ctggatgctg gtgggctacg gttttgtggc ggcggtgctg ccggtgtggt
    12541 tgctgctggc cccgcgtgac tatctctcga ccttcctgaa aatcgggact atcgtcggtc
    12601 tggcggtagg cattttgatt atgcgcccga cgctgaccat gcctgcgctg accaaatttg
    12661 tcgatggcac tggcccggta tggaccggta acctgttccc gttcctgttt atcaccatcg
    12721 cctgtggtgc ggtgtctggc ttccatgcgc tgatctcttc cggcaccacg ccgaagatgc
    12781 tggcgaacga agggcaggcg tgctttatcg gctacggtgg gatgttgatg gaatccttcg
    12841 tggcgattat ggcgctagtt tctgcctgta tcatcgatcc gggcgtgtat tttgccatga
    12901 acagcccaat ggcggtgctg gctccggcag ggacggcgga tgtagtcgct tctgccgcgc
    12961 aggtggtgag tagctggggc tttagcatta caccagatac gctcaaccag attgccagcg
    13021 aagtgggtga acagtcgatc atttcccgtg cgggcggtgc gccgacgttg gcggtgggga
    13081 tggcttacat cctgcacggt gcgctgggtg gtatgatgga tgtggcgttc tggtatcact
    13141 tcgccattct gtttgaagct ctgtttattc tgacggcggt agatgcgggt acgcgtgctg
    13201 cgcgctttat gttgcaggat ctgctgggcg tggtgtctcc tggcctgaag cggaccgatt
    13261 cactgcctgc taacctgctg gcaacagcgc tgtgcgtact ggcgtggggc tacttcctcc
    13321 atcagagcgt ggtcgatccg ctgggcggta ttaacactct gtggccgctg tttggtattg
    13381 ccaaccagat gctggcaggg atggcgctga tgctctgtgc cgtggtgttg ttcaagatga
    13441 aacgtcaacg ttacgcctgg gtggcgctgg taccaacggc ctggctgctg atttgtaccc
    13501 tgaccgctga ctggctgaaa gcgtttagcc cggatgcgaa agtcggcttc ctggtcattg
    13561 ctaacaagtt ccaggcaatg atcgacagcg gcaatattcc gtcgcagtat actgagtcac
    13621 agctggcgca actggtgttc aacaaccgtc tggatgccgg gttaaccatc ttctttatgg
    13681 tggtcgtggt ggtcctggca ctgttctcga ttaagacggc acttgcggca ttgaaagatc
    13741 cgaagccaac ggcgaaagaa acgccgtatg agccgatgcc ggaaaatgtc gaggagatcg
    13801 tagcgcaggc gaaaggcgcg cactaaagtc agagtgaggg gggatgttgg cgaatgttgg
    13861 catagtgccg agggttcccc ctcaccctaa ccctctcccc gatggggcga ggggactgac
    13921 cgagtgcgtt gatagcattt gtaggccgga taaagcgttc acgccgcatc cggcactctt
    13981 tcagcaacat ggttagcgga ggccaagatg tttgattcac tggcaaaagc cggaaaatat
    14041 ttaggtcagg cggcgaagct gatgattggt atgcctgatt acgacaacta tgtcgaacat
    14101 atgcgggtta atcatcccga tcaaacgccg atgacctacg aggagttttt ccgtgagcgg
    14161 caggacgcgc gctacggtgg aaaaggcggc gcgcgctgct gctaaatttg cacgtcaggc
    14221 tttaaacgat tccacttttt cgaacgctgc acgcaacgta tctggtgtca gcgtgactgg
    14281 caggtaatga atggactcca ccgggcgcaa ggtgtgggca atcactttgt cgatctccgc
    14341 ctgattattg atatccacct ccagctccgc cagcgtagtc ggcagatgaa aacgctgata
    14401 cgccccggtt aattgcgcca gcacatcatc ctgacccagc aaggcgcttt gcaccagaat
    14461 tccgtaagcg actttggtgc cgtgaagaaa cttctcggtt tgcggcagga cagtcagacc
    14521 gttatgcacg gcatgagccg ccgccacacg cgtaaaacga tcgcccagac caccgaccat
    14581 cccaccacca gcaataatag catccaccac atcgcaaaat gattgcgtta actgctgatt
    14641 ttgctgatcg gcaagtgcct gttcgctact gtttaacaag acgtcgcgaa tggcctgcgc
    14701 attattgatc cccagccgca cggttagcgg caacgtttct ggttgcggag ccagcaccac
    14761 cgcttcatac catttcgcca gcgtgtcacc gatccccgcc agcagatatt gttgcggtgc
    14821 attgaggata atctccggtt ccaccagcac cataaaattg gcgtcgtcga aaatctcata
    14881 atgcagcgcc tgtccggcat cgttatacca gacggagagc ggtgtccagg ctgcgcaggt
    14941 ggcggcgatc gtcggagcgg caacaaatgg cagaccgaga cggcgggcga gggctttcgc
    15001 ggtgtcgagc agtgcgccgc cgccgacgcc aatcaccacg ctgcgatcgt caccggactc
    15061 agccgccagt tgctgtacat cgctttcgct gcaatgacca cgaaacaaaa tatgctttgc
    15121 ccctggaagt ccaaacgctg gcggaagttt ggtttgcgcc gcagcaatgg cgcgtttgcc
    15181 gtagatccac accgcgcgag aaagttgttc atcagtgaaa aaattgtgca ggtgattgaa
    15241 acttcctgga tgtgaaaagt agttagccgg gccgacgacc acgcggatag gattgtgagg
    15301 catgttgtgt tgtccttatt gttttattta gacatctaaa cgtcttgatt gccaaatact
    15361 ggcatcgtgt tatagtgcct tcaacacgca acttcgtcag gtacaataaa aatgacaaat
    15421 aaccctctga ttccacaaag caaacttcca caacttggca ccactatttt cacccagatg
    15481 agcgcgctgg cgcagcaaca ccaggcgatt aacctgtcgc aaggctttcc tgattttgat
    15541 ggtccgcgct atttacagga gcggctggcg taccacgttg cccagggggc aaaccaatac
    15601 gcgcccatga ccggcgtgca ggccttgcgc gaggcgattg ctcagaaaac ggaacgtttg
    15661 tatggctatc aaccagatgc cgatagcgat atcaccgtaa cggcaggggc gacggaagcg
    15721 ttatacgcgg cgattaccgc actggtgcgc aatggcgatg aagtgatttg ttttgatccc
    15781 agctatgaca gttacgcccc cgccatcgcg ctttctgggg gaatagtgaa acgtatggca
    15841 ctgcaaccac cgcattttcg cgttgactgg caggaatttg ccgcattatt aagcgagcgc
    15901 accagactgg tgatcctcaa cactccgcat aaccccagtg caactgtctg gcagcaggct
    15961 gatttcgccg ctttgtggca ggcgatcgcc gggcacgaga tttttgtcat tagcgatgaa
    16021 gtctacgagc acatcaactt ttcacaacag ggccatgcca gtgtgctggc gcatccgcag
    16081 ctgcgtgagc gggcagtggc ggtttcttca tttggcaaga cctatcatat gaccggctgg
    16141 aaagtgggtt attgtgttgc gccagcgccc atcagcgccg aaattcgcaa ggtacatcag
    16201 tatctgacct tttcggtgaa taccccggca cagctggcgc ttgctgatat gctacgtgca
    16261 gaacctgagc attatcttgc gttaccggac ttttatcgcc agaagcgcga tattctggtg
    16321 aatgctttaa atgaaagccg gctggagatt ttaccgtgtg aaggtacata ctttttgctg
    16381 gtggattaca gcgcggtttc taccctggat gatgttgagt tttgccagtg gctgacgcag
    16441 gagcacggcg tagcggcgat tccgctgtcg gtgttttgcg ccgatccctt cccacataaa
    16501 ctgattcgtc tctgttttgc caagaaggaa tcgacgttgc tggcagcagc tgaacgcctg
    16561 cgccagcttt agctatttaa ccgtccaggc gcgggaatat tgacggtcgg caaacaactc
    16621 ttgcaggccg ttaatttgct tcagtcgcag cacttcgtcg ctgtccattc ccagctcttt
    16681 gccaatttta ttatcatccc atcccaactg gctaagttca cggacgattt ccgacatggc
    16741 ggtgatttga tggcgtccac gagcgcgatt atgacgaata gtcgcggcga tgcgttgatt
    16801 acgcgtacct tccagacagg tcaccggcaa ataacctttt aagcgtaatt ttaatgatga
    16861 tgagccttta ccaatttcat ggcggtgaaa accatccacg atttccatgg c
//
LOCUS       DAJTRW010000082        16846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRW010000082 DAJTRW010000000
VERSION     DAJTRW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..233)
                     /locus_tag="OO326_002964"
     CDS             complement(<1..233)
                     /locus_tag="OO326_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5967273.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGR"
     gene            complement(413..2854)
                     /gene="rnr"
                     /locus_tag="OO326_002965"
     CDS             complement(413..2854)
                     /gene="rnr"
                     /locus_tag="OO326_002965"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5967274.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2893..3318)
                     /gene="nsrR"
                     /locus_tag="OO326_002966"
     CDS             complement(2893..3318)
                     /gene="nsrR"
                     /locus_tag="OO326_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5967275.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3523..4821)
                     /gene="purA"
                     /locus_tag="OO326_002967"
     CDS             complement(3523..4821)
                     /gene="purA"
                     /locus_tag="OO326_002967"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5967276.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4925..5122)
                     /locus_tag="OO326_002968"
     CDS             complement(4925..5122)
                     /locus_tag="OO326_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5967277.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5204..6208)
                     /gene="hflC"
                     /locus_tag="OO326_002969"
     CDS             complement(5204..6208)
                     /gene="hflC"
                     /locus_tag="OO326_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5967278.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6211..7470)
                     /gene="hflK"
                     /locus_tag="OO326_002970"
     CDS             complement(6211..7470)
                     /gene="hflK"
                     /locus_tag="OO326_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5967279.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7556..8836)
                     /gene="hflX"
                     /locus_tag="OO326_002971"
     CDS             complement(7556..8836)
                     /gene="hflX"
                     /locus_tag="OO326_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5967280.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8912..9220)
                     /gene="hfq"
                     /locus_tag="OO326_002972"
     CDS             complement(8912..9220)
                     /gene="hfq"
                     /locus_tag="OO326_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5967281.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9306..10256)
                     /gene="miaA"
                     /locus_tag="OO326_002973"
     CDS             complement(9306..10256)
                     /gene="miaA"
                     /locus_tag="OO326_002973"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5967282.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10249..12096)
                     /gene="mutL"
                     /locus_tag="OO326_002974"
     CDS             complement(10249..12096)
                     /gene="mutL"
                     /locus_tag="OO326_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5967283.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12106..13443)
                     /gene="amiB"
                     /locus_tag="OO326_002975"
     CDS             complement(12106..13443)
                     /gene="amiB"
                     /locus_tag="OO326_002975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5967284.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13462..13923)
                     /gene="tsaE"
                     /locus_tag="OO326_002976"
     CDS             complement(13462..13923)
                     /gene="tsaE"
                     /locus_tag="OO326_002976"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5967285.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13895..15442)
                     /gene="nnr"
                     /locus_tag="OO326_002977"
     CDS             complement(13895..15442)
                     /gene="nnr"
                     /locus_tag="OO326_002977"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5967286.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLIRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15441..16580
                     /gene="queG"
                     /locus_tag="OO326_002978"
     CDS             15441..16580
                     /gene="queG"
                     /locus_tag="OO326_002978"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5967287.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16563..16616)
                     /locus_tag="OO326_002979"
     CDS             complement(16563..16616)
                     /locus_tag="OO326_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967288.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3823 a         4799 c         4226 g         3998 t
ORIGIN      
        1 cgtcctggct tcacgcgggc gataatgcct tgatgcacgg caccgtcgct tttctcgtcg
       61 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      121 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      181 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      241 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      301 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      361 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      421 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
      481 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
      541 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
      601 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
      661 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
      721 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
      781 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
      841 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
      901 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
      961 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1021 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1081 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1141 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1201 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1261 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1321 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1381 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1441 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     1501 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     1561 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     1621 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     1681 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     1741 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     1801 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     1861 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     1921 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     1981 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2041 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2101 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2161 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2221 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2281 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2341 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2401 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     2461 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     2521 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     2581 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     2641 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     2701 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     2761 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     2821 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     2881 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     2941 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3001 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3061 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3121 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3181 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3241 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3301 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3361 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3421 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     3481 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     3541 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     3601 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     3661 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     3721 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     3781 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     3841 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     3901 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     3961 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4021 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4081 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4141 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4201 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4261 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4321 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4381 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4441 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     4501 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     4561 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     4621 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     4681 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     4741 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     4801 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     4861 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     4921 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     4981 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5041 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5101 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5161 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5221 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5281 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5341 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5401 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     5461 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     5521 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     5581 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     5641 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     5701 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     5761 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     5821 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     5881 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     5941 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6001 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6061 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6121 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6181 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6241 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6301 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6361 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6421 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     6481 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     6541 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     6601 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     6661 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     6721 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     6781 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     6841 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     6901 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     6961 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7021 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7081 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7141 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7201 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7261 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7321 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7381 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7441 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     7501 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     7561 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     7621 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     7681 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     7741 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     7801 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     7861 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     7921 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     7981 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8041 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8101 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8161 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8221 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8281 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8341 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8401 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     8461 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     8521 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     8581 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     8641 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     8701 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     8761 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     8821 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     8881 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     8941 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9001 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9061 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9121 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9181 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9241 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9301 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9361 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9421 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
     9481 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
     9541 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
     9601 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
     9661 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
     9721 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
     9781 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
     9841 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
     9901 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
     9961 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10021 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10081 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10141 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10201 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10261 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10321 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10381 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10441 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    10501 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    10561 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    10621 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    10681 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    10741 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    10801 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    10861 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    10921 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    10981 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11041 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11101 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11161 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11221 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11281 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11341 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11401 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    11461 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    11521 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    11581 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    11641 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    11701 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    11761 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    11821 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    11881 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    11941 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12001 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12061 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12121 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12181 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12241 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12301 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12361 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12421 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    12481 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    12541 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    12601 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    12661 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    12721 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    12781 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    12841 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    12901 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    12961 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13021 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13081 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13141 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13201 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13261 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13321 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13381 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13441 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    13501 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    13561 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    13621 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    13681 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    13741 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    13801 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    13861 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    13921 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    13981 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14041 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14101 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14161 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14221 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14281 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14341 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14401 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    14461 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    14521 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcggatca
    14581 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    14641 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    14701 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    14761 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    14821 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    14881 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    14941 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15001 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15061 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15121 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15181 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15241 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15301 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15361 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15421 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    15481 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    15541 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    15601 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    15661 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    15721 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    15781 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    15841 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    15901 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    15961 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16021 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16081 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16141 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16201 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16261 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16321 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16381 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16441 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    16501 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    16561 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    16621 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    16681 cttatttatc aataggttaa aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    16741 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    16801 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAJTRW010000083        16396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRW010000083 DAJTRW010000000
VERSION     DAJTRW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="OO326_002980"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="OO326_002980"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5967289.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OO326_002981"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OO326_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5967290.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="OO326_002982"
     CDS             2342..2557
                     /locus_tag="OO326_002982"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967291.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="OO326_002983"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="OO326_002983"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5967292.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4456..5850
                     /gene="galP"
                     /locus_tag="OO326_002984"
     CDS             4456..5850
                     /gene="galP"
                     /locus_tag="OO326_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5967293.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5927..6424
                     /locus_tag="OO326_002985"
     CDS             5927..6424
                     /locus_tag="OO326_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5967294.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6519..7226
                     /gene="endA"
                     /locus_tag="OO326_002986"
     CDS             6519..7226
                     /gene="endA"
                     /locus_tag="OO326_002986"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5967295.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7306..8037
                     /gene="rsmE"
                     /locus_tag="OO326_002987"
     CDS             7306..8037
                     /gene="rsmE"
                     /locus_tag="OO326_002987"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5967296.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8050..9000
                     /gene="gshB"
                     /locus_tag="OO326_002988"
     CDS             8050..9000
                     /gene="gshB"
                     /locus_tag="OO326_002988"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5967297.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9109..9672
                     /locus_tag="OO326_002989"
     CDS             9109..9672
                     /locus_tag="OO326_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5967298.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9672..10088
                     /gene="ruvX"
                     /locus_tag="OO326_002990"
     CDS             9672..10088
                     /gene="ruvX"
                     /locus_tag="OO326_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5967299.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10264..11244)
                     /locus_tag="OO326_002991"
     CDS             complement(10264..11244)
                     /locus_tag="OO326_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5967300.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11262..11966
                     /gene="yggS"
                     /locus_tag="OO326_002992"
     CDS             11262..11966
                     /gene="yggS"
                     /locus_tag="OO326_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5967301.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11984..12550
                     /gene="yggT"
                     /locus_tag="OO326_002993"
     CDS             11984..12550
                     /gene="yggT"
                     /locus_tag="OO326_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5967302.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12547..12837
                     /locus_tag="OO326_002994"
     CDS             12547..12837
                     /locus_tag="OO326_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5967303.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12845..13438
                     /locus_tag="OO326_002995"
     CDS             12845..13438
                     /locus_tag="OO326_002995"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5967304.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13431..14567
                     /gene="hemW"
                     /locus_tag="OO326_002996"
     CDS             13431..14567
                     /gene="hemW"
                     /locus_tag="OO326_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5967305.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14881..15844
                     /locus_tag="OO326_002997"
                     /pseudo
     CDS             14881..15844
                     /locus_tag="OO326_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15889..16392
                     /locus_tag="OO326_002998"
     CDS             15889..16392
                     /locus_tag="OO326_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5967306.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4063 a         4222 c         4124 g         3987 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctacgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagaccattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacgctac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgagtctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accaaggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gccgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacgga ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccggcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca actgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct tacatcattg cagatgacag cgtttaatct atgatgatat aactcaatta
     4321 ttttcatgcc cttaaatcat aactaagata aatgttagtg taagcgatta cactgatgtg
     4381 atttgcttca catcttttta cgtcgtactc acctatctta attcacaata aaaaataact
     4441 atattggagg gcatcatgcc tgacgctaaa aaacaggggc ggtcaaacaa ggcaatgacg
     4501 tttttcgtct gcttccttgc cgctctggcg ggattactct ttggcctgga tatcggtgta
     4561 attgctggcg cactgccgtt tattgcagat gaattccaga taacttcgca cacgcaagaa
     4621 tgggtcgtaa gctccatgat gttcggtgcg gcagtcggtg cggtgggcat cggctggctc
     4681 tcctttaaac tcgggcgcaa aaagagcctg atgatcggcg caatcctgtt tgttgccggt
     4741 tcactgttct ctgcggctgc gccaaacgtt gaagtactga ttctttcgcg cgttctgctg
     4801 gggctggcgg tgggtgtggc ctcttatacc gcaccactgt acctgtcaga aattgcgccg
     4861 gaaaaaattc gcggcagtat gatctcgatg tatcagttga tgatcactat cgggatcctc
     4921 ggtgcttatc tttctgatac cgccttcagc tacaccggtg catggcgctg gatgctgggt
     4981 gtgattatca tcccggcaat tttgctgctg attggtgtct tcttcctgcc agacagccca
     5041 cgttggtttg ccgccaaacg ccgttttgtt gatgccgaac gcgtgctgct acgcctgcgt
     5101 gacaccagcg cggaagcgaa acgcgaactg gatgaaatcc gtgaaagttt gcaggttaaa
     5161 cagagtggct gggcgctgtt taaagagaat agcaacttcc gccgcgcggt gttccttggc
     5221 gtactgttac aggtaatgca gcaattcacc gggatgaacg tcatcatgta ttacgcgccg
     5281 aaaatcttcg aactggcggg ttataccaac accaccgagc aaatgtgggg gacagtgatt
     5341 gtcggcctga ccaacgtact tgccaccttt atcgcaatcg gccttgttga ccgctgggga
     5401 cgtaaaccaa cgctaacgct gggcttcctg gtgatggctg ctggcatggg cgtactcggt
     5461 acaatgatgc atatcggtat ccactctccg tcggcgcagt atttcgccat cgccatgctg
     5521 ctgatgttta ttgtcggttt tgccatgagt gccggtccgc tgatttgggt actgtgctcc
     5581 gaaattcagc cgctgaaagg ccgcgatttt ggcatcacct gctccaccgc caccaactgg
     5641 attgccaaca tgatcgttgg cgcaacgttc ctgaccatgc tcaacacgct gggtaacgcc
     5701 aacaccttct gggtgtatgc ggctctgaac gtactgttta tcctgctgac attgtggctg
     5761 gtaccggaaa ccaaacacgt ttcgctggaa catattgaac gtaatctgat gaaaggtcgt
     5821 aaactgcgcg aaatcggcgc tcacgattaa tctcctcagg cttcctcccg tcgcggagga
     5881 agccgcctct tgcagtcatc ttttcttcgc tctatcctct gccgctatga aaacatcccg
     5941 tctccctatc gccatccaac aggccgttat gcgccgcctg cgggaaaaac tcgcccaggc
     6001 caacctgaag ctagggcgta actacccgga gccaaaactc tcttacaccc agcgcggaac
     6061 ctccgccgga acagcctggc tggaaagcta tgaaatccgc ctcaatcccg ttttgctgtt
     6121 ggaaaacagt gaagctttta ttgaagaagt ggtaccgcac gaactggcac atttgctggt
     6181 atggaaacat ttcggccgcg tagcgccaca tggcaaagag tggaagtgga tgatggaaag
     6241 cgtgctgggc gttcccgccc gtcgtacgca tcagttcgaa ctgcaatccg tgcgtcgcaa
     6301 caccttcccc taccgctgta agtgccagga acatcagctt accgtacgcc gccataatcg
     6361 cgtagttcgt ggcgaggcca tctatcgctg tgttcactgc ggtgaacagc tggttgcgaa
     6421 ataaccacct gaactatcag gaactttcct gatctggctg attgcatacc aaaacaactt
     6481 tcgctacgtt gctggctcgt tttaacacgg agtaagtgat gtaccgttat ttgtctattg
     6541 ctgcggtggt actgagcgca gcattttccg gcccgacgtt ggccgaaggt atcaatagtt
     6601 tttctcaggc gaaagccgcg gcggtaaaag tccacgctga tgcgcccggt acgttttatt
     6661 gcggatgtaa aattaactgg cagggcaaaa aaggcgttgt tgatctgcaa tcgtgcggct
     6721 atcaggtgcg caaaaatgaa aaccgcgcca gccgcgtaga gtgggaacac gtcgttcccg
     6781 cctggcaatt cggtcaccag cgccagtgct ggcaggacgg tggacgtaaa aactgcgcta
     6841 aagatccggt ctatcgcaag atggaaagcg atatgcataa cctgcagccg tcagtcggcg
     6901 aggtgaatgg cgatcgcggc aattttatgt acagccagtg gaatggcggc gaaggccagt
     6961 acggtcaatg cgccatgaag gtcgatttca aagaaaaagc tgccgaacca ccagcgcgtg
     7021 cacgcggtgc cattgcgcgc acctacttct atatgcgcga ccaatacaac ctgacactct
     7081 ctcgccagca aacgcagctg ttcaacgcat ggaacaagat gtatccggtt accgactggg
     7141 agtgcgagcg cgatgaacgc atcgcgaagg tgcagggcaa tcataacccg tatgtgcaac
     7201 gcgcttgcca ggcgcgaaag agctaaccta cactagcggg attctttttg ttaaccccta
     7261 ccccacgcgt acaaccgcgt ggggagacga cgcggatttt taactatgcg tatcccccgc
     7321 atttatcatc ctgaaccact gaccagccat tctcacatcg cgctttgcga agatgccgcc
     7381 aaccatatcg ggcgcgtact gcgcatgggg ccggggcagg cgttgcaatt gtttgacggt
     7441 agcaaccagg tctttgacgc cgaaattacc agcgccagca aaaaaagcgt ggaagtgaag
     7501 gtgctggaag gccagatcga cgatcgcgaa tctccgctgc atattcacct cggtcaggtg
     7561 atgtcgcgtg gtgaaaaaat ggaatttact atccagaaat cgatcgaact cggtgtaagc
     7621 ctcattacgc cacttttttc tgagcgctgc ggcgttaaac tggatagtga acgtctgaac
     7681 aagaagcttc agcagtggca gaagattgca attgctgcct gtgagcagtg tggtcgtaac
     7741 cgggtgccgg aaatccgtcc agcgatggat ctggaagcct ggtgtgcaga gcaggatgaa
     7801 ggactgaaac tgaatcttca cccgcgcgcc agtaacagca tcaatacgtt gcctttaccg
     7861 gttgaacgcg tccgcctgct gattggcccg gaaggcggtt tatcggcaga tgaaattgcc
     7921 atgactgccc gctatcaatt tactgatatc ctgttgggac ctcgcgtttt gcgtacagag
     7981 acaactgcgc tcaccgccat taccgcgcta caagtacgat ttggcgattt gggctaacgg
     8041 agaagaataa tgatcaagct cggcatcgtg atggacccca tcgcaaacat caacatcaag
     8101 aaagattcca gtttcgctat gttgctggaa gcacagcgtc gtggttacga acttcactat
     8161 atggagatgg gcgatctgta tctgatcaat ggcgaagccc gcgcccatac ccgcacgctg
     8221 aacgtgaagc agaactacga agagtggttt tcgttcgtcg gtgaacagga tctgccgctg
     8281 gccgatctcg atgtgatcct gatgcgtaaa gacccgccgt ttgataccga gtttatctac
     8341 gcgacctata ttctggaacg tgccgaagag aaagggacgc tgatcgttaa caagccgcag
     8401 agcctgcgcg actgtaacga gaaactgttt accgcctggt tctctgactt aacgccagaa
     8461 acgctggtta cgcgcaataa agcgcagcta aaagcgttct gggagaaaca cagcgacatc
     8521 attcttaagc cgctggacgg tatgggcggc gcgtcgattt tccgcgtgaa agaaggcgat
     8581 ccaaacctcg gcgtgattgc cgaaaccctg actgagcatg gcacttgcta ctgcatggcg
     8641 caaaattacc tgccagccat taaagatggc gacaaacgcg tgctggtggt ggatggcgag
     8701 ccggtaccgt actgcctggc gcgtattccg caggggggcg aaacccgtgg caatctggct
     8761 gccggtggtc gcggtgaacc tcgtccgctg acggaaagtg actggaaaat cgcccgtcag
     8821 atcgggccga cgctgaaaga aaaagggctg atttttgttg gtctggatat catcggcgac
     8881 cgtctgactg aaattaacgt caccagccca acctgtattc gtgagattga agcagagttt
     8941 ccggtgtcga tcaccggaat gttaatggat gccatcgaag cacgtttaca gcagcagtaa
     9001 cccaccttag cgagaaggat ctcgttgaga ctctgagtga cagcgccctt ctttccacgc
     9061 atactgggcg ctgttgcttt tttgaaccag gaaacagaac ctctgacaat gaatttacag
     9121 catcactttc ttattgccat gcctgctctc caggatccga ttttccgtcg ttccgtggtc
     9181 tacatttgcg aacataatac caatggtgca atggggatca tcgtcaacaa gccgctggaa
     9241 aatctcaaaa ttgaagggat tctggaaaag ctgaagatca cgccggagcc gcgtgatgaa
     9301 tcaatccgtc tggataaacc ggttatgctc ggcggcccac tggctgaaga tcgcgggttt
     9361 attttgcata ctccgccctc caattttgct tccagcattc gcatttcaga caacacggtg
     9421 atgaccactt cccgcgatgt gctggaaacg ctcggcaccg ataaacaacc gtctgacgta
     9481 ttggtggctc tgggctatgc ctcctgggaa aaaggtcaac tggaacaaga aattctcgat
     9541 aacgcgtggc taacggcccc ggcggatctg aatattctgt tcaaaacgcc gattgccgac
     9601 cgctggcgcg aggcggcaaa actgattggt gtggatattc tcaccatgcc tggtgtggca
     9661 ggacacgcct gatgagtgga accttactcg ccttcgactt cggcaccaaa agcattggcg
     9721 tagcggtcgg ccaacgcatt accggcaccg ctcgcccttt gcctgcaatt aaagcgcagg
     9781 acggtacgcc ggactggaac ctcatcgaac gtttactgaa agagtggcag cctgacgaaa
     9841 tcatcgtcgg tttgccgctg aatatggacg gcaccgaaca gccgttaacc gccagagcgc
     9901 gcaaatttgc caaccgtatt catggtcgtt tcggtgttga agtaaagctc catgacgagc
     9961 gtcttagcac tgtggaagcc cgttccggtc tttttgaaca gggcggctat cgggcgctca
    10021 acaaaggcaa agttgactct gcctctgcgg ttattattct cgaaagctat ttcgagcagg
    10081 gatattaagg cgatctaaaa gcctggcgga tagtcgttac cgccatcttg ccggatgcgg
    10141 cgtgaacgcc ttatccagca cacatctggc agcggctgta ggtctgataa gacgcgttag
    10201 cgtcgcatca gacatttatt gcctttatag gccctataaa cttgcgcatc gggcatggca
    10261 acgtcacaaa cgcccttccc ccacccgctg ctgataactc tgctgaaacg ttaacatccc
    10321 caccttctgc ccggtttgaa taacatgcgg taactggtgg gttttccctt cgcgaatcaa
    10381 attccccacc gcgggagtgt taatcagtaa ttcaaacagc gccacgcgtc cttcctgttt
    10441 atccacttcc agcttttgtg acagcactgc ccgtaaacta cccgcaagtt gattacgtac
    10501 gggatctttt tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc
    10561 acgtgtatgt aaggttgcca gcaccagatg cccggtttct gccgccgtca gcgccagacg
    10621 gattgtctca ctgtcacgta gctctccgag caaaatcaca tcaggatctt cccgcaatgc
    10681 agcccgcaac cccgatgcga acgtcataca gtgcaaacca atttcccgct gctggatcaa
    10741 acatcgctgg ctggcataga gatattccac aggatcttcc agcgtcagaa tatgcgcatc
    10801 ggcatgttga ttaagatagc caaccatcgc cgccagcgtg gtagatttgc cactccccgt
    10861 agcccccgtc accagaatca ggccattctc gctcttgagt aattccggca ataccggtgg
    10921 tgcaccaagc tgttcgagct gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat
    10981 accttgccgt tgcgcgaacg cactgccacg caaccgctgg ttttccgcca gcgacacggc
    11041 aaaatccagc tggccatttt ccagcaatat tgcccgctga tcgtcatcca gccactcccg
    11101 cagtagctct tcgacgtccg gcgcatcaaa cggcgcagct tccattctgc cacgaatgcg
    11161 ccatcgtgcg ggccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag
    11221 ggccacaatt tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata
    11281 acctggcaca ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag
    11341 aagaaattac gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca
    11401 ttgatgccgg gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc
    11461 gccactttca ggaactgggc gtaacaggat tagaatggca ttttattggc ccgttgcagt
    11521 ctaataaaag ccgcctagtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc
    11581 gcattgctac ccgcctcaac gagcagcgcc cggcagaact tccccctctt aacgtactga
    11641 ttcaaattaa tattagtgat gaaaacagta agtccgggat tcaactggca gaactggacg
    11701 agctggcagc ggcggtcgct gaactaccgc gtttacgtct gcgcgggctg atggcaatcc
    11761 ctgcgcttga gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat
    11821 ttgccggact gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg
    11881 atatggaagc cgccattgcg gcaggtagca cgatggttcg tatcggcact gcaatttttg
    11941 gtgcgcgtga ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt
    12001 cctgctttca acggtcattg agctgtatac aatggtgctg ttattacgca tctggatgca
    12061 gtgtgctcat tgtgattttt acaccccctt ctcacagttt gtagtgaagg tagcgcagcc
    12121 aattatcggg ccactgcgcc gcgttattcc ggcaatgggt cccattgaca gcgcctcgct
    12181 gctggttgcc tatattctca gttttatcaa agccattgtg ctgtttgaag tggtgacctt
    12241 cctgccaatc atctggattg ccggtttact gattctgctg aaaaccatcg gcttgctgat
    12301 tttctgggtc ctgctggtga tggcgattat gagctgggta agccagggtc gtagcccgat
    12361 tgaatacgtg ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcttgctacc
    12421 ggcaatgggt gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgccatcaa
    12481 tatgggtgtc gcagaagtat tacaggcaac cggaaacatg ctgctgccgg ggctgtggat
    12541 ggcgttatga gtgccgtaac agttaatgat gacggtctgg ttttacggct ctatattcag
    12601 ccgaaagcca gccgtgattc tattgtcggt ttacatggcg acgaagttaa agtcgccatt
    12661 accgcgccgc cggttgacgg ccaggccaac agtcatctgg tgaagtttct cggtaagcaa
    12721 ttccgggttg ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa
    12781 attaaaatca ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta
    12841 tcctatgcaa aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc
    12901 gctgcttagc gactttggtc ttgatatcgt ggcccaaaca gacctcggcg ttgattccgc
    12961 tgaagaaacc ggcctaacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa
    13021 agtgaccggt ttaccggcaa ttgccgacga ctctggtctg gcggtagatg cgctgggtgg
    13081 cgcgccgggt atttactccg cgcgttattc cggtgaagac gcgaccgatc aaaagaatct
    13141 gcaaaaactg ttggaaacac tgaaagacgt accggacgac caacgtcagg cgcgtttcca
    13201 ctgcgtgctg gtatatctgc gtcacgcgga agatccgact ccgctggtat gccacggcag
    13261 ctggccgggc gtgattactc gcgaaccagc gggcactggt ggctttggtt atgatccaat
    13321 cttcttcgta ccttccgaag ggaaaaccgc cgccgaactg acccgcgaag aaaagagcgc
    13381 catttcccac cgtggtcagg cgttgaaact gctgctggac gctttacgta atggttaaat
    13441 taccgccgct gagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg
    13501 atttcaactc tcacgcgttg aaaggagaag tgccgcacga cgattatgtt cagcatctgc
    13561 tttgcgatct ggacaatgat gtggcttacg ctcagggccg tgaagtaaag acaatcttta
    13621 ttggcggtgg tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctagacggcg
    13681 tgcgtgcgcg tttgccgctg gcagcggatg cagaaattac tatggaagcg aacccaggca
    13741 cggtagaagc ggatcgcttt gtcgattatc agcgtgctgg tgtaaaccgc atctctattg
    13801 gtgtgcagag ttttagcgaa gaaaagctga aacgacttgg gcgtattcat ggcccgcaag
    13861 aagcgaaacg agcggcaaag ctggcgagcg gtttagggtt acgtagcttt aaccttgatt
    13921 tgatgcatgg actaccggat caatcactgg aagaggcgct tggcgatcta cgccaggcca
    13981 ttgaactgaa tccgccgcat ctttcctggt atcaactgac tatcgaacct aatacgctgt
    14041 ttggctcgcg accgccggtg ctgccggacg atgacgcgct gtgggatatt ttcgaacagg
    14101 ggcatcagtt attaaccgca gcgggttatc agcaatatga aacttccgct tacgccaaac
    14161 ccggttatca gtgccagcac aatctcaact actggcgctt tggtgactac atcggtattg
    14221 gctgcggcgc gcacggcaaa gtgaccttcc cggatgggcg cattctgcgt accactaaaa
    14281 cgcgtcatcc tcgtggtttt atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag
    14341 ccgcagataa gccgtttgag ttctttatga atcgcttccg tctgctggag gccgcgccgc
    14401 gcgtggagtt tattgcgtat accgggcttt gcgaagatgt gattcgccca cagttagacg
    14461 aggcgattgc ccagggttat ctcaccgaat gtgcggatta ctggcagata acggaacatg
    14521 gtaaattatt cttaaactca cttcttgaat tattcctcgc tgagtaatca ttaatgcgcc
    14581 tgcgggcgca ttaatcactt cttttatccc cccgttaatt aacctcggca gaaatatatt
    14641 tcttactgaa taaatatcga cgacatttat tatatatatt tagtgaatat ttactaatca
    14701 ttcattgatt ataaatatgt acaacctggc gcacaaattt aatatttaat ttctgacttt
    14761 atttcaagtt gtgataaaca tagacaagtt gtcagacagg ttaaccaatt tgtcgcacaa
    14821 atcactgata agcacaatta gatatcaacc aatttccatc cttcaacagg gagttttttt
    14881 atgaaaatga ggtcatttac ccgctcatta gtctgcgcat ccttgctggc tctggttagc
    14941 actggcgtaa acgccgcaga aaaagtggcg ttaaagttag cacacaacct ggaaagaagt
    15001 catgttgtac atcagagttt tgaagagctg gcgaaagagg taaaacagct gtcaaaaggt
    15061 aatatggtga tccgtattta tcccagcagc cagatgggta atgcgcgaga aactatggaa
    15121 ttgttgcaaa atggcgcact ggatatgacc aaaggttccg ccagtgatct ggagtctttt
    15181 gataatattt atgctatcta taatttgccg ttcttattaa gttgtttttg gtgaagttgg
    15241 taaagaaatt atggactcaa caaaagacaa aggctttttt gctttatcag cttatgtagc
    15301 gggtactcgc agtttctatg cgaaaaaacc gattacaaag ccagaagatc ttaaaggact
    15361 gaaaattcgt gttcagccaa gtccgaccac catcaaaatg attgaactga tgggtggttc
    15421 acccactcca atttcatttg gcgaagttta tactgcgatg cagcaaggtg tagtagatgg
    15481 cgcagaaaat aacgtgcctt cctggatgca gactcgacat attgaaattg ccaaagtttt
    15541 ttctgaagat gaacacgcct caattcctga ctttttggtt atctccacca aaacatggaa
    15601 taaattaacc ccagaacagc aacaaatcct cgaaacagcg gcgaagaaat ccgaagctta
    15661 tcaacaaaaa ttgtgggaaa aaattgacgc tgacacacgt gctcaggcga aagctatggg
    15721 gggtgaaatt gtaaaagtgg ataaagcgcc attccgtaaa gccgtgcaac ctctgtttga
    15781 cgacttcaaa aaagatccaa aacaagctgc tttgttagag aaattcgaca acgctgctca
    15841 ataatattcc gggccagcct ctggccctga ttttccagga tgcttacaat gatatttaac
    15901 cgcctgaaac ttgcggtaga ccgcgtcatt gccacgtttt ctgtcgccgt aatgcttgct
    15961 ctggttgtat gcgtagtctg gcaagtattt agccgctacg tacttaacca gccaagtaca
    16021 ttaaccgatg agctggcacg ctttttgatg atctgggtag gattattagg tgctgcatat
    16081 accgtcggcg cgcagcgaca tctctctatt gatttatttg ctctcgccct gaataagcgc
    16141 aaacaattac ttcttagtat agtcattaac gttctcattt taggttttgc cggttcggtc
    16201 attgtgactg gtggcctgaa attaattgat aaaacactgg caacatcaca ggtatctgcc
    16261 gctatgcaaa taccgatggg atacgtatat atcattctgc cattaagtgg agtggtaatg
    16321 atgttttatg ccttgtgttt tattaatcaa agcattcaac aactgaaaca acctgcgcag
    16381 gaggccagtt aatgga
//
LOCUS       DAJTRW010000084        16156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRW010000084 DAJTRW010000000
VERSION     DAJTRW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO326_002999"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO326_002999"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5967307.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO326_003000"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO326_003000"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5967308.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO326_003001"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO326_003001"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5967309.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO326_003002"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO326_003002"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5967310.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO326_003003"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO326_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO326_003004"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO326_003004"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5967311.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO326_003005"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO326_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO326_003006"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO326_003006"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5967312.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO326_003007"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO326_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5967313.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10182)
                     /locus_tag="OO326_003008"
                     /pseudo
     CDS             complement(8973..10182)
                     /locus_tag="OO326_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10403..10831
                     /gene="uspG"
                     /locus_tag="OO326_003009"
     CDS             10403..10831
                     /gene="uspG"
                     /locus_tag="OO326_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5967314.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10952..12517)
                     /gene="ahpF"
                     /locus_tag="OO326_003010"
     CDS             complement(10952..12517)
                     /gene="ahpF"
                     /locus_tag="OO326_003010"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5967315.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12762..13325)
                     /gene="ahpC"
                     /locus_tag="OO326_003011"
     CDS             complement(12762..13325)
                     /gene="ahpC"
                     /locus_tag="OO326_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5967316.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13697..14443
                     /gene="dsbG"
                     /locus_tag="OO326_003012"
     CDS             13697..14443
                     /gene="dsbG"
                     /locus_tag="OO326_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5967317.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14653..15555
                     /locus_tag="OO326_003013"
     CDS             14653..15555
                     /locus_tag="OO326_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5967318.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15702..15905
                     /locus_tag="OO326_003014"
                     /pseudo
     CDS             15702..15905
                     /locus_tag="OO326_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(15924..>16156)
                     /locus_tag="OO326_003015"
     CDS             complement(15924..>16156)
                     /locus_tag="OO326_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5967319.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         4013 a         4170 c         3894 g         4079 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc
     9361 cgtggtgctg cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg
     9421 acctgccgtt tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg
     9481 gatcgcgccg tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa
     9541 aatctgttcc gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccagc
     9601 accatagact gcaacgcttg aaccttgttg gatctgcaca ttttttgctg cctgccatgc
     9661 cgttggcaga atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt
     9721 aaacggcccc acattccctt tcgggacgcg gacatattcc gcctgcccgc caggaacgcc
     9781 accatacagg tgactataac caaacaatgc cgcgggagca ggtatctgtt ttttattgag
     9841 cgcagcgcct ttacccgcat tggtattttc gcaggcggca tattgctgta atcgacagaa
     9901 aaaacagtcg ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt
     9961 ttttacgtcc ttcccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc
    10021 atgtttaacc tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt
    10081 taccgtaata cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc
    10141 tacctgaacg tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt
    10201 tcaaaaaaga gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag
    10261 gttttgggag aattcctagt gttcagtgag aatatattaa tcacagaaac cgttcgtaag
    10321 gaaaaagaac cagacaaata tcagggataa atattataat catggagaat gatttgttca
    10381 tgattaacag ggagaaaggg ttatgtataa ggcaatcatt atgccagttg atgtatttga
    10441 aatggaattg agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt
    10501 tattcatcta cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc
    10561 tgatgtgcgt cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat
    10621 ggtcagccac ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt
    10681 gcgggatgaa gtcaatgagt tggcagaaga actgggggct gatgttgtag ttatcggttc
    10741 tcgcaaccca tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca
    10801 cgccaatctg cctgtactgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct
    10861 ggaacatccc ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg
    10921 ctaagcaatt gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt
    10981 caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg
    11041 gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttca catttcgcat
    11101 caatgataat ctcgcccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg
    11161 gcagcagacc aatctggaca aaaataccgg ccagttcgat gttgtgaata ttgccgctga
    11221 cacgatctcg atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt
    11281 gcgcattcag aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt
    11341 cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt
    11401 cgattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa
    11461 acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt agtgcgatac tgatcttcgc
    11521 ccggaacgtt catgttgcgc cattttgcac ccgtcgccac gataatgctg cgtgctttca
    11581 gtaccgcgcc agaagctgtt tcaatctggt gtagaccacc ttcaaccgct gccgggatca
    11641 gtttgctggc gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca
    11701 gtgcgcctgc caacttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa
    11761 cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac
    11821 gtgcggagta aattgctgcc gctgcacccg ccgggccgga accaacgatt aatacgtcat
    11881 aagcatcacg cttgttcagc tcttctgccg cacgattttc cgcgccagta tcaattttgg
    11941 caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg
    12001 ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg
    12061 cagtgtgctt gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg
    12121 ggcagttatg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc
    12181 gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg
    12241 ccagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt
    12301 tggaacctgg gttggtgatc aggaaagacg gcttacgcac tggcaagctg ttatcttctt
    12361 taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg
    12421 atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt
    12481 aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca
    12541 tcatcatgca agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag
    12601 aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga
    12661 gcaaggcgac tagcctgtga atccccggga gcttacacaa gtaagtgacc ggggtgagcg
    12721 ggcgacgcca acgccgctat ggcgtgaaag acgaaggaaa tttagatttt accaaccagg
    12781 tccagagacg gagccagagt tgcttcacct tctttccatt tagccgggca aacttcacct
    12841 gggtgagaag ctacgtactg agctgctttg attttacgca gcagatcaga cgcgtcacgg
    12901 ccaatgcctt cagcggtaac ttcgattgcc tggatgatac cctgcgggtc aacaacgaag
    12961 gtcgcacggt cagccagacc ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg
    13021 ccagtcgggt cgccgatcat cgcatatttg attttagcga tggtttcaga gctgctgtgc
    13081 catgctttgt gggtgaagtg agtatcggta gatactgcgt atacgtctac gcccagtttc
    13141 tgcagttctt cgtagtggtc agcaacgtca cccagttcgg tcgggcatac gaaagtaaag
    13201 tcagccgggt agaagaagaa gacgctccag cggccttcgg tatctttttc ggtgatttcg
    13261 atgaattcgc cgtttttgaa tgcctggttt ttaaaaggtt taattttggt gttaatcaag
    13321 gacatctata cttcctccgt gttttcgatg agatgtaagg taaccaattt ttgccgattc
    13381 ggctaatgcg tttccattat caaatcgata agttttacct tacaaccttc gtaagacaac
    13441 tttgtgaact ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt tccaggtgac
    13501 cgtgtaatct gaactaccct ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc
    13561 cttacaaatg caacaacgac atggattaca acaccctcat aaacaaaggg caatcacctg
    13621 atctaagctc ttacctatga cagtgatagg ttatgccttt tactcgactt ttgcactgac
    13681 tgaaaagaac aaattaatgt taaaaaagat acttttactg gctctgcttc ctgcaatcgc
    13741 cttcgcagag gaacttcctt ccccagtaaa agcgattgaa aaacagggca ttacaatcat
    13801 caaaacgttc gatgcccccg gaggaatgaa aggttatctc ggaaagtatc aggatatggg
    13861 cgtcaccatc tacctgactc ccgatggtaa gcacgctatc tctggttaca tgtacaacga
    13921 gaaaggtgaa aacctgagta acacacttat cgaaaaagaa atttacgcac cagccggacg
    13981 cgaaatgtgg caacggatgg aacaatccca ctggctcctc gacggtaaaa aagatgcgcc
    14041 ggtcattgtc tacgtcttct ccgatccgtt ctgcccatat tgtaaacagt tctggcagca
    14101 ggcgcgcccg tgggtagatt ctggcaaagt gcaattaaga acattgttgg ttggggttat
    14161 caagccagaa agcccggcga cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac
    14221 ctggcaacaa tatgaagcct ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag
    14281 tacagagcaa atgaaagtgt taagtgacaa tgagaaactg atggacgatc tgggggcaaa
    14341 tgtcacgccg gctatctatt acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt
    14401 gcccgatcag aaaacgctta atatcattat gggaaataaa taaaaggtaa ttcatggatg
    14461 gttgaactat atccctgctt ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc
    14521 taagtggctg tgctatttta gaaaaactta atcctgagtt aagcaaaaaa acataatcaa
    14581 taaaaataca tacgcaaaaa acattgatta agtgaatata ccatggaaga aaaatataaa
    14641 ccggagtagt gtatggccaa tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc
    14701 atatttgagt gtatttacca gcatttaagc attagcaaag ctgctgaatc actatatatc
    14761 accccatcag ccgtgagcca gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta
    14821 ttcattcgct caggaaaagg tatcgcgccg acgacaacag ggttgaatct gcatcaccat
    14881 cttgagaaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
    14941 aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg
    15001 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
    15061 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
    15121 ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt
    15181 tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa
    15241 gagttcaccc aattaatagc caaatcagca ggagttgatg atattcaaat ggaaatagac
    15301 gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat
    15361 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ctaccaccgt
    15421 gactttctta atttaaaaga gataaaacta gaacatccgt taccttcaat aaagctttac
    15481 atttcctata ataaatcttc tttaaataat ttggtgttct cccgatttat tgaccgtcta
    15541 aatgagagtt tttgatatat aactatagac agctactata actttcatct atttattcac
    15601 agcgcgaatg aaaaataaaa cactctgctt tacagtgttt actttataat taacagtctt
    15661 atctcactgt tcaccctgta acgttcagag agcgtcttca gatgtctatt tataaaattc
    15721 ctcttccgct caatattctg gaagctgcac gagagcgtat tacctagaca ctcaacaccc
    15781 taccccgcgt ttgtgtctca ttttctggcg gcaaagactc tggtttaatg ctgcatctga
    15841 cagccgaact tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc aactgggagg
    15901 cgcaatgaat cgccacggat aatctagaca cttccgagcc gttgataata ctggttttca
    15961 tattctgtcg gtgacatctg ttcgctagaa ccatgccgac gcttactgtt ataaaacatt
    16021 tcgatgtaat caaaaatatc actgcgggct tcttcccgcg ttccgtagat ctttttcttt
    16081 atccgttcac gtttcaacaa ctggaaaaaa ctttctgcaa ccgcattatc atggcagtta
    16141 ccgcgacggc tcatgc
//
LOCUS       DAJTRW010000085        15586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRW010000085 DAJTRW010000000
VERSION     DAJTRW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..78
                     /locus_tag="OO326_003016"
     CDS             <1..78
                     /locus_tag="OO326_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967320.1"
                     /translation="DDGLRFAIREGGRTVGAGVVAKVLG"
     gene            261..455
                     /gene="bfd"
                     /locus_tag="OO326_003017"
     CDS             261..455
                     /gene="bfd"
                     /locus_tag="OO326_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5967321.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            528..1004
                     /gene="bfr"
                     /locus_tag="OO326_003018"
     CDS             528..1004
                     /gene="bfr"
                     /locus_tag="OO326_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5967322.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1001..1468)
                     /locus_tag="OO326_003019"
                     /pseudo
     CDS             complement(1001..1468)
                     /locus_tag="OO326_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1847..2158
                     /gene="rpsJ"
                     /locus_tag="OO326_003020"
     CDS             1847..2158
                     /gene="rpsJ"
                     /locus_tag="OO326_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5967323.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2191..2820
                     /gene="rplC"
                     /locus_tag="OO326_003021"
     CDS             2191..2820
                     /gene="rplC"
                     /locus_tag="OO326_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5967324.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2831..3436
                     /gene="rplD"
                     /locus_tag="OO326_003022"
     CDS             2831..3436
                     /gene="rplD"
                     /locus_tag="OO326_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5967325.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3433..3735
                     /gene="rplW"
                     /locus_tag="OO326_003023"
     CDS             3433..3735
                     /gene="rplW"
                     /locus_tag="OO326_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5967326.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3753..4574
                     /gene="rplB"
                     /locus_tag="OO326_003024"
     CDS             3753..4574
                     /gene="rplB"
                     /locus_tag="OO326_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5967327.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4591..4869
                     /gene="rpsS"
                     /locus_tag="OO326_003025"
     CDS             4591..4869
                     /gene="rpsS"
                     /locus_tag="OO326_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5967328.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4884..5216
                     /gene="rplV"
                     /locus_tag="OO326_003026"
     CDS             4884..5216
                     /gene="rplV"
                     /locus_tag="OO326_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5967329.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5234..5935
                     /gene="rpsC"
                     /locus_tag="OO326_003027"
     CDS             5234..5935
                     /gene="rpsC"
                     /locus_tag="OO326_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5967330.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5948..6358
                     /gene="rplP"
                     /locus_tag="OO326_003028"
     CDS             5948..6358
                     /gene="rplP"
                     /locus_tag="OO326_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5967331.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6358..6549
                     /gene="rpmC"
                     /locus_tag="OO326_003029"
     CDS             6358..6549
                     /gene="rpmC"
                     /locus_tag="OO326_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5967332.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6549..6803
                     /gene="rpsQ"
                     /locus_tag="OO326_003030"
     CDS             6549..6803
                     /gene="rpsQ"
                     /locus_tag="OO326_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5967333.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6968..7339
                     /gene="rplN"
                     /locus_tag="OO326_003031"
     CDS             6968..7339
                     /gene="rplN"
                     /locus_tag="OO326_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5967334.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7350..7664
                     /gene="rplX"
                     /locus_tag="OO326_003032"
     CDS             7350..7664
                     /gene="rplX"
                     /locus_tag="OO326_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5967335.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7679..8218
                     /gene="rplE"
                     /locus_tag="OO326_003033"
     CDS             7679..8218
                     /gene="rplE"
                     /locus_tag="OO326_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5967336.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8233..8538
                     /gene="rpsN"
                     /locus_tag="OO326_003034"
     CDS             8233..8538
                     /gene="rpsN"
                     /locus_tag="OO326_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5967337.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8572..8964
                     /gene="rpsH"
                     /locus_tag="OO326_003035"
     CDS             8572..8964
                     /gene="rpsH"
                     /locus_tag="OO326_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5967338.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8977..9510
                     /gene="rplF"
                     /locus_tag="OO326_003036"
     CDS             8977..9510
                     /gene="rplF"
                     /locus_tag="OO326_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5967339.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9520..9873
                     /gene="rplR"
                     /locus_tag="OO326_003037"
     CDS             9520..9873
                     /gene="rplR"
                     /locus_tag="OO326_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5967340.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9888..10391
                     /gene="rpsE"
                     /locus_tag="OO326_003038"
     CDS             9888..10391
                     /gene="rpsE"
                     /locus_tag="OO326_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5967341.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10395..10574
                     /gene="rpmD"
                     /locus_tag="OO326_003039"
     CDS             10395..10574
                     /gene="rpmD"
                     /locus_tag="OO326_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5967342.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10578..11012
                     /gene="rplO"
                     /locus_tag="OO326_003040"
     CDS             10578..11012
                     /gene="rplO"
                     /locus_tag="OO326_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5967343.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11020..12351
                     /gene="secY"
                     /locus_tag="OO326_003041"
     CDS             11020..12351
                     /gene="secY"
                     /locus_tag="OO326_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5967344.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12383..12499
                     /gene="rpmJ"
                     /locus_tag="OO326_003042"
     CDS             12383..12499
                     /gene="rpmJ"
                     /locus_tag="OO326_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5967345.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12646..13002
                     /gene="rpsM"
                     /locus_tag="OO326_003043"
     CDS             12646..13002
                     /gene="rpsM"
                     /locus_tag="OO326_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5967346.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13019..13408
                     /gene="rpsK"
                     /locus_tag="OO326_003044"
     CDS             13019..13408
                     /gene="rpsK"
                     /locus_tag="OO326_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5967347.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13442..14062
                     /gene="rpsD"
                     /locus_tag="OO326_003045"
     CDS             13442..14062
                     /gene="rpsD"
                     /locus_tag="OO326_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5967348.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14088..15077
                     /gene="rpoA"
                     /locus_tag="OO326_003046"
     CDS             14088..15077
                     /gene="rpoA"
                     /locus_tag="OO326_003046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5967349.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15118..15501
                     /gene="rplQ"
                     /locus_tag="OO326_003047"
     CDS             15118..15501
                     /gene="rplQ"
                     /locus_tag="OO326_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5967350.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4014 a         3659 c         4153 g         3760 t
ORIGIN      
        1 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
       61 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      121 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      181 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      241 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      301 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      361 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      421 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      481 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      541 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      601 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      661 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      721 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      781 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      841 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      901 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      961 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1021 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1081 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1141 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1201 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1261 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1321 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1381 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1441 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1501 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1561 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1621 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1681 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1741 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1801 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1861 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1921 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1981 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2041 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2101 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2161 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2221 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2281 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2341 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2401 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2461 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2521 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2581 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2641 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2701 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2761 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2821 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2881 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2941 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3001 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3061 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3121 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3181 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3241 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3301 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3361 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3421 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3481 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3541 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3601 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3661 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3721 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3781 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3841 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3901 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3961 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4021 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4081 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4141 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4201 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4261 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4321 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4381 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4441 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4501 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4561 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4621 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4681 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4741 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4801 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4861 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4921 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4981 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5041 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5101 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5161 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5221 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5281 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5341 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5401 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5461 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5521 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5581 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5641 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5701 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5761 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5821 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5881 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5941 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6001 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6061 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6121 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6181 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6241 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6301 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6361 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6421 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6481 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6541 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6601 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6661 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6721 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6781 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6841 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6901 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6961 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7021 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7081 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7141 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7201 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7261 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7321 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7381 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7441 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7501 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7561 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7621 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7681 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7741 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7801 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7861 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7921 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7981 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8041 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8101 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8161 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8221 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8281 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8341 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8401 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8461 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8521 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8581 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8641 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8701 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8761 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8821 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8881 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8941 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9001 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9061 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9121 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9181 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9241 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9301 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9361 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9421 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9481 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9541 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9601 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9661 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9721 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9781 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9841 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9901 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9961 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10021 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10081 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10141 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10201 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10261 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10321 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10381 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10441 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10501 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10561 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10621 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10681 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10741 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10801 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10861 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10921 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10981 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11041 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11101 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11161 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11221 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11281 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11341 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11401 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11461 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11521 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11581 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11641 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11701 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11761 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11821 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11881 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11941 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12001 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12061 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12121 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12181 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12241 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12301 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12361 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12421 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12481 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12541 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12601 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12661 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12721 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12781 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12841 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12901 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12961 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13021 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13081 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13141 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13201 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13261 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13321 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13381 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13441 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13501 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13561 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13621 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13681 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13741 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13801 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13861 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13921 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13981 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14041 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14101 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14161 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14221 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14281 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14341 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14401 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14461 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14521 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14581 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14641 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14701 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14761 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14821 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14881 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14941 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15001 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15061 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15121 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15181 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15241 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15301 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15361 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15421 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15481 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15541 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTRW010000086        15262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRW010000086 DAJTRW010000000
VERSION     DAJTRW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO326_003048"
     CDS             complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO326_003048"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5967351.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO326_003049"
     CDS             complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO326_003049"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5967352.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO326_003050"
     CDS             complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO326_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5967353.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3829..4761
                     /gene="rfaD"
                     /locus_tag="OO326_003051"
     CDS             3829..4761
                     /gene="rfaD"
                     /locus_tag="OO326_003051"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5967354.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4771..5817
                     /gene="rfaF"
                     /locus_tag="OO326_003052"
     CDS             4771..5817
                     /gene="rfaF"
                     /locus_tag="OO326_003052"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5967355.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5821..6813
                     /gene="rfaC"
                     /locus_tag="OO326_003053"
     CDS             5821..6813
                     /gene="rfaC"
                     /locus_tag="OO326_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5967356.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6810..8018
                     /gene="rfaL"
                     /locus_tag="OO326_003054"
     CDS             6810..8018
                     /gene="rfaL"
                     /locus_tag="OO326_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5967357.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8054..9196)
                     /locus_tag="OO326_003055"
     CDS             complement(8054..9196)
                     /locus_tag="OO326_003055"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5967358.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9205..10218)
                     /locus_tag="OO326_003056"
     CDS             complement(9205..10218)
                     /locus_tag="OO326_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5967359.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO326_003057"
     CDS             complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO326_003057"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5967360.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO326_003058"
                     /gene_synonym="rfaI"
     CDS             complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO326_003058"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5967361.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO326_003059"
     CDS             complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO326_003059"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5967362.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12816..13940)
                     /locus_tag="OO326_003060"
     CDS             complement(12816..13940)
                     /locus_tag="OO326_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5967363.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="OO326_003061"
     CDS             complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="OO326_003061"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5967364.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4344 a         3436 c         3130 g         4352 t
ORIGIN      
        1 tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca gctatataaa
       61 agatcagcgc aattacgaac tctttattac actgacagga ttttacataa ttaacagatt
      121 atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca gctcagaata
      181 actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc atcgatagag
      241 aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc cgccatcttg
      301 taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc tttaaagatc
      361 actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc aatacgaccg
      421 ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga catttccaga
      481 ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag gttttctttc
      541 gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg gtattcgtta
      601 acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc agccataata
      661 cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa cgacagcgcg
      721 gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga gatattgtcg
      781 gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca gcccggacgg
      841 ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg acagttgcga
      901 cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt gaagcctttc
      961 acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac catcggcacc
     1021 gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt cccgcagatg
     1081 gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac agggacgtcg
     1141 gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc acatcctcag
     1201 gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg cgtaatttgc
     1261 tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt cggaacgacc
     1321 ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc aaatttctgc
     1381 gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc cgccagggta
     1441 aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc
     1501 aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac aattgctggt
     1561 gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc aaccacttct
     1621 ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag cgtgccggtg
     1681 atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc attttcaccg
     1741 acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt atctgccaga
     1801 tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc atcggtggca
     1861 atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc aagctcctgc
     1921 atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc atcaataata
     1981 gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag cgtttcaaac
     2041 aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat ccccaggaat
     2101 gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc
     2161 gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat cagatccgga
     2221 tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct tccatcagcc
     2281 acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa caacccttcc
     2341 gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg cattgcgatt
     2401 ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg acgagatgat
     2461 gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc tctcaacacc
     2521 tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg attttactgc
     2581 attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca gttggcatca
     2641 aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc tcagcgcctg
     2701 catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc attttcctct
     2761 ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga caaaaatgcc
     2821 ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca gaactaattt
     2881 atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa tatatacatg
     2941 attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc ctttttttat
     3001 agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca atcctcgagt
     3061 tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg ttttcactaa
     3121 acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca aaacatactc
     3181 tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg taaatgactc
     3241 atctttttga attttttcga tacgacttct gatatatcta aatttctttt ttatatcgat
     3301 aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt tcaaatcggg
     3361 agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt aactaaaata
     3421 aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc aatcccctct
     3481 accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta ataagaccat
     3541 taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta tattatccat
     3601 tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac tattcacatg
     3661 caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt tgcaattagc
     3721 atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat gattacagac
     3781 attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat gatcatcgtt
     3841 accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga taaaggcatc
     3901 accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa cctggtggat
     3961 ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat ggctggcgaa
     4021 gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac caccgagtgg
     4081 gatggcaagt atatgatgga taacaactat caatactcca aagagctgct gcactactgt
     4141 ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg cggacgcacc
     4201 tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg ttactcaaaa
     4261 ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca gattgttggc
     4321 ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc
     4381 gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc
     4441 gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa cctgtggttc
     4501 ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga atccttccag
     4561 gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata cattccgttc
     4621 ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac aaatctgcgc
     4681 gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga atacatggcc
     4741 tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg cccgtcttgg
     4801 gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag
     4861 gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg gatgccggaa
     4921 gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc
     4981 aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt accaaactcc
     5041 ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc
     5101 gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc ctggccgcta
     5161 atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac cgcacaagat
     5221 ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa atcatatacc
     5281 tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc gggtgcggag
     5341 tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa gcagctgatt
     5401 gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc gggcaatgag
     5461 attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc gggggaaaca
     5521 cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac taacgattct
     5581 ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta tggtccaagt
     5641 agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct gattaccggc
     5701 tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt gatcgacatt
     5761 actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga agcctgacgg
     5821 atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac gttgcccgca
     5881 ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt ggaagaaggg
     5941 ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt
     6001 cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc gtttcgtgaa
     6061 gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg
     6121 gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca aaccgctcgc
     6181 gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca gcagcacgcc
     6241 gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc gcaaacccag
     6301 ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc tggcgaatat
     6361 gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga acactggcga
     6421 gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg gggcgcgccg
     6481 catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga agtattgccg
     6541 aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg
     6601 gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac ggtttatgga
     6661 ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg tcgttctaca
     6721 agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa tcttgaaata
     6781 ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc tctctcgaga
     6841 aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt accacctatt
     6901 ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca ataacgacaa
     6961 tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca tttctttttg
     7021 gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc cctgatgcag
     7081 gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta tgcacaatag
     7141 caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct
     7201 catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact
     7261 atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg
     7321 tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt
     7381 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
     7441 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
     7501 tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc acacataagg
     7561 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
     7621 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
     7681 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
     7741 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
     7801 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
     7861 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
     7921 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
     7981 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
     8041 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
     8101 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
     8161 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
     8221 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
     8281 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
     8341 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg
     8401 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     8461 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     8521 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     8581 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt
     8641 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     8701 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     8761 ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     8821 taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat
     8881 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     8941 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac
     9001 tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg
     9061 gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc
     9121 acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata
     9181 ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa ttgctgtaaa
     9241 aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt tgtgctcatg
     9301 ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat aaatattacg
     9361 aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta taccagtata
     9421 atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat ttttttcttc
     9481 aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt tagcatgatg
     9541 tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta taattgactc
     9601 atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt cccgtaaatt
     9661 aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac acaaacgctc
     9721 agcgctttta ctttgcatag catctacatc aattaccact gccccatatt catctttaaa
     9781 ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat caagatataa
     9841 taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat aaatagatac
     9901 tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat gatataattt
     9961 aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact ctttgtctaa
    10021 gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa gaattgaagc
    10081 aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga cattaaacgt
    10141 ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt taaattgagt
    10201 aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc tcttcaatcc
    10261 tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata taagatatgt
    10321 atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat caatgcgatc
    10381 ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc ttaaaccttt
    10441 ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat gctgatgcaa
    10501 ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca aatactcatt
    10561 taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga aatttaatgt
    10621 tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac cacgtacccg
    10681 atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt ttaagaaacg
    10741 ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac gagcagtgtc
    10801 aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt taaccctgca
    10861 agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta tgaaaacttt
    10921 caacccattt atcgttttat tatatatcat aatgaattat tttaacctta aatctttaga
    10981 agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa tgcttttgtt
    11041 taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt
    11101 ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc ggataatcag
    11161 cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga
    11221 ctggattatc aactttgtct tttaattcat agttaatact atatcgggta ttatatttct
    11281 ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg
    11341 tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac gaaatgttat
    11401 ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat ccagaagcta
    11461 actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct ctttcagcta
    11521 caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta atactaccct
    11581 tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag aaataatctg
    11641 caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt aatgacttca
    11701 gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt gctaaatcag
    11761 aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat tcacaagaga
    11821 tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa agaaaatttc
    11881 tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga taatgaaaat
    11941 tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt ctgtatccaa
    12001 aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt tccctgattt
    12061 ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga atgtctttaa
    12121 gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc aggccaatat
    12181 tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct ggaacgcgcg
    12241 tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct tccttaccgg
    12301 aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta atgcccgcag
    12361 catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt acgcgaacat
    12421 ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt ggtgtcagat
    12481 cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg ccgaatgcca
    12541 ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc cattcgcggt
    12601 ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct ttcagggtcg
    12661 tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc agagtacggc
    12721 gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca aaaggatctt
    12781 tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc acccgttatg
    12841 atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata atgtcgcgca
    12901 ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa aatctcattc
    12961 aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc gacaatataa
    13021 tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat cgcttccagc
    13081 agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc cgccgccatt
    13141 aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac gccgcgtttt
    13201 tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag caatgtgttg
    13261 tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc acctttacgc
    13321 gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc attcttctta
    13381 cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg ataaatcccc
    13441 ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa atcggcgatt
    13501 tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt tgcccgctca
    13561 aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt ttcctgcgct
    13621 actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg cattttgttg
    13681 aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca ggcaaaatac
    13741 tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac ttcaaataca
    13801 tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc tcgggcggcg
    13861 actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa atatttatat
    13921 aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt aatttatcga
    13981 cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt cggtcacgct
    14041 gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg ttatttgacc
    14101 agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc gtattaactg
    14161 cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca tgatcgatta
    14221 acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt ggcgtctggc
    14281 atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg gacgtcagaa
    14341 cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg gcgttgtccc
    14401 agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt ccaacaccag
    14461 cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc atggttgtct
    14521 gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt tccaccacat
    14581 ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca gactgccgat
    14641 gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt atagcaacca
    14701 tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat accttgatga
    14761 gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt atgccgtaga
    14821 gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa agcagcacat
    14881 cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta gttaataaca
    14941 tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta tccacaagta
    15001 actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct accgccccag
    15061 gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt gtatcacttc
    15121 tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca ataaagaaat
    15181 caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga ttccttcctg
    15241 cacgtacagc agaaatcaat ga
//
LOCUS       DAJTRW010000087        15235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRW010000087 DAJTRW010000000
VERSION     DAJTRW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1163)
                     /locus_tag="OO326_003062"
     CDS             complement(<1..1163)
                     /locus_tag="OO326_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5967365.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLG"
     gene            complement(1362..1446)
                     /locus_tag="OO326_003063"
     tRNA            complement(1362..1446)
                     /locus_tag="OO326_003063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1410..1412),aa:Leu,seq:caa)
     gene            1642..2661
                     /gene="ahr"
                     /locus_tag="OO326_003064"
     CDS             1642..2661
                     /gene="ahr"
                     /locus_tag="OO326_003064"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5967366.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2790..4292
                     /locus_tag="OO326_003065"
     CDS             2790..4292
                     /locus_tag="OO326_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5967367.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4411..5493)
                     /gene="lptG"
                     /locus_tag="OO326_003066"
     CDS             complement(4411..5493)
                     /gene="lptG"
                     /locus_tag="OO326_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5967368.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5493..6593)
                     /gene="lptF"
                     /locus_tag="OO326_003067"
     CDS             complement(5493..6593)
                     /gene="lptF"
                     /locus_tag="OO326_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5967369.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6860..8371
                     /gene="pepA"
                     /locus_tag="OO326_003068"
     CDS             6860..8371
                     /gene="pepA"
                     /locus_tag="OO326_003068"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5967370.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8505..8948
                     /gene="holC"
                     /locus_tag="OO326_003069"
     CDS             8505..8948
                     /gene="holC"
                     /locus_tag="OO326_003069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5967371.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8948..11803
                     /gene="valS"
                     /locus_tag="OO326_003070"
     CDS             8948..11803
                     /gene="valS"
                     /locus_tag="OO326_003070"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5967372.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11857..13053)
                     /locus_tag="OO326_003071"
     CDS             complement(11857..13053)
                     /locus_tag="OO326_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5967373.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13246..13749
                     /locus_tag="OO326_003072"
     CDS             13246..13749
                     /locus_tag="OO326_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5967374.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13795..14211)
                     /gene="rraB"
                     /locus_tag="OO326_003073"
     CDS             complement(13795..14211)
                     /gene="rraB"
                     /locus_tag="OO326_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5967375.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14373..>15235
                     /gene="argF"
                     /locus_tag="OO326_003074"
     CDS             14373..>15235
                     /gene="argF"
                     /locus_tag="OO326_003074"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5967376.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGK"
BASE COUNT         3923 a         3896 c         3925 g         3491 t
ORIGIN      
        1 tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg ctcctggtga
       61 ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa cgaactacaa
      121 gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc cattacgcgc
      181 aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc gcccacaaaa
      241 atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag ggcctgccgc
      301 gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg cttgactcct
      361 tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc tatttctgac
      421 cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag ttcaaccgcc
      481 agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc aggtatacgg
      541 ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc tgcaatctct
      601 tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat cgcggtagta
      661 cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc tttaatgggt
      721 acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc agtaaagaga
      781 ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt ctgattgctg
      841 gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg ttctacctta
      901 acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg agcctcatcc
      961 cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc cagcatcttc
     1021 tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg gtgaaccagc
     1081 aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt agccgaacga
     1141 accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg tgggtacaaa
     1201 cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga tgtagattga
     1261 atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac tggattttag
     1321 gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga aggccggact
     1381 cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg atttcgccac
     1441 ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca cgccaccatg
     1501 caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta cactcagcga
     1561 tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac ttcccaaaca
     1621 acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa agaagcgggc
     1681 ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga tgttgaagtg
     1741 caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa cgaatgggga
     1801 ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt ggcactcggg
     1861 agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg
     1921 cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg cgagcaaggt
     1981 gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa
     2041 tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct gttgtgcggc
     2101 ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag ccgcgttggg
     2161 gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc aatgggatgc
     2221 gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc gatgggtgcc
     2281 gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg gcagtttgat
     2341 ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga ggcgctgacc
     2401 tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt tccggccttt
     2461 acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc ttatgagctg
     2521 cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga actgttcccg
     2581 atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg ctaccgagta
     2641 gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac ttaaatctct
     2701 ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc cgttgcctat
     2761 actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt gcccgcacgc
     2821 cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg attactggcg
     2881 caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg tcggaaatcg
     2941 gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg caggcaggaa
     3001 cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac tggcaaccgc
     3061 atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg gtgcgggcga
     3121 cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac gatgtgcaat
     3181 ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg ctgctcgact
     3241 ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc ttccagaatc
     3301 agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta ctgtcgctgg
     3361 aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa gactggatgc
     3421 gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa ctttatcaga
     3481 tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat gaacaattgc
     3541 cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag gcacatctgc
     3601 tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata aggcttattc
     3661 gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt ccggataatg
     3721 tgttcggcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg ccaaaagatc
     3781 agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt gataccgaaa
     3841 aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat gcaaaaggaa
     3901 gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg gggccggtga
     3961 cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa tatgaagatg
     4021 aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc agtaccgagc
     4081 agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt attcttggtg
     4141 gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa gatggtgtgg
     4201 tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta cgcgggatgt
     4261 tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc ccctcaccct
     4321 aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt tgtcaacaat
     4381 aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca gccacaggct
     4441 gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga tgccgtaaac
     4501 caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga agctgatacc
     4561 tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa acgacagcgc
     4621 catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca tgttgagctg
     4681 ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt gcaaaccact
     4741 gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg tgaggtcggt
     4801 tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg tcagatcaga
     4861 ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg cggtagcggc
     4921 ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga tgccacctaa
     4981 cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt tcgcccataa
     5041 accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc
     5101 catctgttcg ccctgcggcg cgacccattc gccaatcgcc atcgtcagca agaccagtgg
     5161 aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag aagcctgcat
     5221 caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc caagaagagc
     5281 cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat acattcctgc
     5341 gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa atttgataat
     5401 gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa tagttttacc
     5461 gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg cgaaaaactg
     5521 gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc caaagccaga
     5581 taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt accgccgttc
     5641 gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc tggcagcatc
     5701 gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat aagtgccatc
     5761 ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc acgatcggtg
     5821 tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc gagcgccacc
     5881 gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa atcacgtaac
     5941 agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga gccgtcgcgc
     6001 agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt accttttggt
     6061 cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc gatgaacagc
     6121 accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc catgccaggg
     6181 ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg tcccgcccac
     6241 atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc cgctttcacc
     6301 agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc ggtatacagt
     6361 ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag ctgcgccatt
     6421 tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc gccgtcaacc
     6481 gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa gatgaagagt
     6541 atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat cactttaaat
     6601 acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt aaacgtcatt
     6661 tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga ttcacctctc
     6721 agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga agtcatcgca
     6781 acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc gttgtcttta
     6841 agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg
     6901 gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga ttgcagaaca
     6961 gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg aactggaagg
     7021 aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg agcgaattct
     7081 ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc aggttattca
     7141 gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct ttctgactga
     7201 actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg agacagcaaa
     7261 agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc gtcgtccgct
     7321 gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg agcgcgcgat
     7381 ccagcacggt caggcgattg ccgccgggat aaaagcagcg aaagatctcg gtaatatgcc
     7441 gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg ctgacagcta
     7501 cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc tgggaatgca
     7561 ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg tgattgagta
     7621 caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt
     7681 cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga agtacgatat
     7741 gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc aactgccgat
     7801 taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg cctatcgtcc
     7861 gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca ccgatgccga
     7921 aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc cggaagcagt
     7981 gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc atattactgg
     8041 tctgatgacg aaccataatc cgctggccca tgaactgatt gccgcgtctg aacaatccgg
     8101 tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg agtccaattt
     8161 tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag gttgcttcct
     8221 gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa ccgcctggcg
     8281 ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac agttcctgtt
     8341 aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac cctaaccctc
     8401 tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat ctttcagggt
     8461 gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa aacgcaacgt
     8521 tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt
     8581 gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc tgtgaagatg
     8641 aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc
     8701 cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc gcctggccgc
     8761 aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt
     8821 ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac
     8881 tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct
     8941 ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt acgagcactg
     9001 ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat
     9061 gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat
     9121 catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg
     9181 tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga
     9241 aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct gggaatggaa
     9301 agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact ccgtcgactg
     9361 ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag aagttttcgt
     9421 tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact gggacccgaa
     9481 actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg
     9541 gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag attatctggt
     9601 ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg ttaacccgga
     9661 agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg
     9721 tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaagat
     9781 cacaccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc tgccgatgat
     9841 caacatcctg acctttgacg gtgatatccg tgaaagcgcc caggtgttcg ataccaaagg
     9901 taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac tcgagcgttt
     9961 tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg aagaaattaa
    10021 accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg aaccgatgct
    10081 gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg aagcggttga
    10141 gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg
    10201 cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta tcccggcatg
    10261 gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc gcaaggaaaa
    10321 taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca cctggttctc
    10381 ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg ccctgcgtca
    10441 gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct ggattgcccg
    10501 catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt
    10561 ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga tgtccaaatc
    10621 caagggtaac gttattgacc cgctggatat ggttgacggt atttcgctgc cagaactgct
    10681 ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac
    10741 cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc gcttcaccct
    10801 ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc tggaaggcta
    10861 ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acacagaagg
    10921 tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc gctggattct
    10981 ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga
    11041 tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact ggtatctgga
    11101 gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta ctcgccatac
    11161 gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac
    11221 cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct
    11281 gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg ccgacaccga
    11341 gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga acatcgcgcc
    11401 gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa
    11461 tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg tgctgcctgc
    11521 cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc tgctgatccc
    11581 gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag aagtggcgaa
    11641 gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct ttgtcgcccg
    11701 cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg cagaagcgaa
    11761 agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac agtgatgaaa
    11821 acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta aagaaggcat
    11881 aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt tattgattaa
    11941 aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt caatcatcca
    12001 tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca ccaacgcaca
    12061 catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac gcccatcatt
    12121 cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac tggtgctaac
    12181 agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca tttccataaa
    12241 ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact catttaatat
    12301 ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta aaaatgcgag
    12361 gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat gtataccgaa
    12421 actggtattg ctccagagaa aactcattac caggctatat aaagtaccat taaaaatacc
    12481 aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac tacttgaact
    12541 attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa tggccattaa
    12601 aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa aactaaatct
    12661 tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg ccataaatat
    12721 aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat ctgctgacac
    12781 tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa aaacattccc
    12841 accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat aaagataacg
    12901 cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg ttaacaacac
    12961 attcaccaca caaataagaa aatatctctc acctgtacca tgaaaaacaa aagaatggga
    13021 cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta aaaaatccat
    13081 tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata ctcttatcag
    13141 taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa gaagagagtg
    13201 gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa taacattgcg
    13261 ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat cgccagcgtc
    13321 atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac cgttgccgat
    13381 ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta ttgggtggtt
    13441 gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg tagtgaagcg
    13501 gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa agggctagcg
    13561 aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa acgctgctat
    13621 ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt gggctttgag
    13681 catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg gatgctacgc
    13741 gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa ttaattagtg
    13801 gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt cgccgttagg
    13861 atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg ccagcgtcat
    13921 cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc
    13981 gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg tcacttcgta
    14041 accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt ctgcggaaag
    14101 atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta attcttcaat
    14161 aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca tggctcattc
    14221 ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc tccacctttg
    14281 tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa ttgagtttta
    14341 attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat aagcatttcc
    14401 tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta gccgcgaagc
    14461 tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa aacatcgcgc
    14521 tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc gcatatgacc
    14581 agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat aaagagtcga
    14641 ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat cgcggctatg
    14701 gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg aacggcctga
    14761 ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag gagcatttac
    14821 ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt aacaacatgg
    14881 gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg gtcgcgccac
    14941 aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca caacaaaatg
    15001 gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct gactttatct
    15061 ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgaa cgcattgcat
    15121 tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac ccggaggtca
    15181 aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc aaaaa
//
LOCUS       DAJTRW010000088        14927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRW010000088 DAJTRW010000000
VERSION     DAJTRW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(470..1411)
                     /gene="fabY"
                     /locus_tag="OO326_003075"
                     /gene_synonym="yiiD"
     CDS             complement(470..1411)
                     /gene="fabY"
                     /locus_tag="OO326_003075"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5967377.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1456..1893)
                     /gene="dtd"
                     /locus_tag="OO326_003076"
     CDS             complement(1456..1893)
                     /gene="dtd"
                     /locus_tag="OO326_003076"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5967378.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1890..2762)
                     /locus_tag="OO326_003077"
     CDS             complement(1890..2762)
                     /locus_tag="OO326_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5967379.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2756..3355)
                     /gene="yihX"
                     /locus_tag="OO326_003078"
     CDS             complement(2756..3355)
                     /gene="yihX"
                     /locus_tag="OO326_003078"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5967380.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3454..4239)
                     /locus_tag="OO326_003079"
     CDS             complement(3454..4239)
                     /locus_tag="OO326_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5967381.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4273..5169)
                     /gene="yihV"
                     /locus_tag="OO326_003080"
     CDS             complement(4273..5169)
                     /gene="yihV"
                     /locus_tag="OO326_003080"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5967382.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5337..6233
                     /gene="yihU"
                     /locus_tag="OO326_003081"
     CDS             5337..6233
                     /gene="yihU"
                     /locus_tag="OO326_003081"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5967383.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6257..7135
                     /gene="yihT"
                     /locus_tag="OO326_003082"
     CDS             6257..7135
                     /gene="yihT"
                     /locus_tag="OO326_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5967384.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7152..8393
                     /gene="yihS"
                     /locus_tag="OO326_003083"
     CDS             7152..8393
                     /gene="yihS"
                     /locus_tag="OO326_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5967385.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8531..9433
                     /locus_tag="OO326_003084"
     CDS             8531..9433
                     /locus_tag="OO326_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5967386.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9632..11668
                     /gene="yihQ"
                     /locus_tag="OO326_003085"
     CDS             9632..11668
                     /gene="yihQ"
                     /locus_tag="OO326_003085"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5967387.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11714..13088
                     /locus_tag="OO326_003086"
                     /pseudo
     CDS             11714..13088
                     /locus_tag="OO326_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13131..14534
                     /locus_tag="OO326_003087"
     CDS             13131..14534
                     /locus_tag="OO326_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967388.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14602..>14927
                     /gene="ompL"
                     /locus_tag="OO326_003088"
     CDS             14602..>14927
                     /gene="ompL"
                     /locus_tag="OO326_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5967389.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3665 a         3848 c         3726 g         3688 t
ORIGIN      
        1 taaattaaca acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc
       61 ctccttccca cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt
      121 aaatgaatga tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta
      181 tagattttaa aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa
      241 atcgctcaaa cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag
      301 ctctacaaat aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca
      361 acgaagaata aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat
      421 gtaaatgacc ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt
      481 cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt
      541 caaacactgc acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt
      601 ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat
      661 ggggtttacc gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc
      721 cgccgaggtg gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca
      781 gcgagaataa actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca
      841 tggtggtgat aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa
      901 gcgggatatg ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa
      961 tatcatccag agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg
     1021 gcgtggtgat ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt
     1081 cacgggcgct acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca
     1141 tcgccatcag cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa
     1201 tggacgcttc attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct
     1261 cgtcgacgac catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg
     1321 gttgatgcag gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt
     1381 cttctgtttg tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca
     1441 accctggaag ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga
     1501 tacctgcata tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg
     1561 ttcgacgaaa tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact
     1621 tgggcgcatc ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact
     1681 gccgcccgcc tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta
     1741 gccgagcaca cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc
     1801 caataacacc aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct
     1861 ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct
     1921 gcttgtttta gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg
     1981 atacaccatg tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa
     2041 atgagctgat atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg
     2101 aacaggagtg cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta
     2161 ggaacaatgc tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc
     2221 agcacgttgt cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa
     2281 ctgatcgcca gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg
     2341 gcgaacgagt aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta
     2401 tcgatggagt acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc
     2461 atcttgttgg aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca
     2521 ggcagaaagt tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga
     2581 aaagcggcga aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca
     2641 taggcaaggt tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt
     2701 tttagccagg cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc
     2761 ataacacctt cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa
     2821 tgcctagctg attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc
     2881 tgggtgaaaa accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca
     2941 tccccagatc ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt
     3001 ccggccagaa ggtggtatgc aggcggttgg tattggaaag caccaccaca cgatgcccct
     3061 gctcacgcag tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct
     3121 gccagccgtg agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct
     3181 ctgcgaacgc ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat
     3241 gaaaactctt cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca
     3301 cacggttaaa gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg
     3361 cctcctttcg cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc
     3421 aaggtatgaa gccaggttaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat
     3481 ttcaatttgg ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa
     3541 gagcgcagtg acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt
     3601 gtgatcggcg accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc
     3661 ttcgtttaca tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac
     3721 cagataatca gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct
     3781 attatgagag cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag
     3841 aatatgcgcc acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc
     3901 ccgggcaaca tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat
     3961 atagtctgcc accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg
     4021 ctcgaacgcc gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat
     4081 caggccttgc tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag
     4141 cagatttgcc agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg
     4201 gtcgtgccgc gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat
     4261 caccctggca ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat
     4321 cccggctcgt ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac
     4381 tgactccgct aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc
     4441 tcccgcacct gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc
     4501 attttccagc cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag
     4561 cgtttgtgcc tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc
     4621 cggttctgaa aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt
     4681 aatgtccccg tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt
     4741 agcgccgtcg tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc
     4801 ctccaaccac tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc
     4861 gcctttggta tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt
     4921 gtaacgggtg ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc
     4981 atcacctacg cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc
     5041 cgtcgccgct ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc
     5101 cgtcggtaac ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac
     5161 acgaatcatg gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt
     5221 ttaaaaagtg acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac
     5281 aagcaaagca tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg
     5341 cagcaatcgc gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc
     5401 agcaagggca tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag
     5461 acaaaggtgc gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca
     5521 ttaccatgtt gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt
     5581 gcgaaggctt atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa
     5641 ccgataaatt gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag
     5701 gccgtacttc tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg
     5761 aacaagttga acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg
     5821 gcggtccggg catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca
     5881 atgcgctttc ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg
     5941 ccgtcaaagt gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa
     6001 acaaagtcct cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc
     6061 ttggcatcgc ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct
     6121 cacgggaggt ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca
     6181 ttctggaaca ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg
     6241 ataaaggaat aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg
     6301 ttttgccatg ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg
     6361 ggcacccgct ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc
     6421 cctctcgcct tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt
     6481 tgagcaaaac gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc
     6541 tggcaacggt attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat
     6601 caaacaagac ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc
     6661 gcagcaacgt ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt
     6721 aagcatcatt gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca
     6781 agcgatcatc gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga
     6841 aatgccgctt tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa
     6901 tgaccatatc aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc
     6961 gcgtgccgta cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt
     7021 ctgggcatcg gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc
     7081 gaaattacaa caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga
     7141 ggataaaagg aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa
     7201 ccgaccgcat ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag
     7261 gcaataaagg gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt
     7321 tgcacgttta ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc
     7381 acggtattaa agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct
     7441 gcgtgaatga cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc
     7501 tgctgggtgc cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt
     7561 acaccattga gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt
     7621 cctgggacga agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg
     7681 cggtggaagc tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg
     7741 cgattcgcgt ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta
     7801 acgaacactt cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc
     7861 atcgcttccg cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc
     7921 tgcacatcca tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag
     7981 atgccaaagg tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg
     8041 ggattgttta taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc
     8101 ctatcgtcga agcaatgggt acggtctacg cgctctacac cgtcactggc gatcgccagt
     8161 atgaaacctg gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa
     8221 atggttcctg gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg
     8281 gcaaacagga tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc
     8341 ccggcatggc tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct
     8401 gaaaataggg tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc
     8461 gtccggtgca gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa
     8521 ggtgccttgt atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc
     8581 gggggattat cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca
     8641 gggttgtcat ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa
     8701 agtgcttatt ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga
     8761 atatcagtta cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg
     8821 gcgagactgg caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc
     8881 gcccagttac ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca
     8941 cacgggtctt tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg
     9001 cgtgggtatt catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca
     9061 gcttccggca aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca
     9121 cgttgatgag ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga
     9181 ccacaccttt aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc
     9241 gctttcggta agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt
     9301 acagcgtcag ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg
     9361 tatcgactta ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg
     9421 acagagaaaa taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct
     9481 aaaggcaaca ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt
     9541 ttttatatat cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac
     9601 aatctcgttc tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga
     9661 ttttcaattt catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt
     9721 aacccataac aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat
     9781 gttccgcggt aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc
     9841 catcgtcagc cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag
     9901 cgccacgctg aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga
     9961 caaccttaac cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta
    10021 cggctgcggc gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac
    10081 cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa
    10141 agaaaatgcg ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac
    10201 gcagaagtat tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc
    10261 ggaataccat gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga
    10321 cacatacatc tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc
    10381 cgactggatt tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa
    10441 gaaactggac accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg
    10501 gtctgggatc cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag
    10561 cgaaaactac ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt
    10621 cctggcctat atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca
    10681 acacggctat ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt
    10741 ttacggcggc gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat
    10801 caaaaagaac atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct
    10861 gcccaccgac acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc
    10921 tgcgctgtgg gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat
    10981 cctcttcttt atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc
    11041 gggcgaccag aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc
    11101 gctgtcgctg gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac
    11161 cctgtttgag atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt
    11221 cacgccgatg atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg
    11281 cgatgcggaa actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc
    11341 ttatctgaaa gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct
    11401 gttcctgcat tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg
    11461 ccgcgacatt ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct
    11521 gccggaggat aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac
    11581 cgttaatgcg cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc
    11641 actgttcgcg tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa
    11701 agggagaaca tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt
    11761 taaagagaaa ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg
    11821 tacgctttat cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg
    11881 cattatcttt ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat
    11941 gttggattcc cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc
    12001 gtcattcccg gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac
    12061 cggtaaaacg gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat
    12121 gaactgctcc tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc
    12181 actggcggca tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt
    12241 cgtgccagtt atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac
    12301 gctgttttca ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg
    12361 ttatgtcgaa acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg
    12421 cgcaattgcc ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg
    12481 ggcgtttaac gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc
    12541 catcctgttg tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct
    12601 gatgcctggc gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat
    12661 ttgggtgctg ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc
    12721 gttctcctgc ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg
    12781 tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca
    12841 ccttctttcg caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc
    12901 aaattggcta tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt
    12961 tgatcttcat ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct
    13021 acagcctgaa cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca
    13081 cggcgtaata ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc
    13141 ataatccact gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg
    13201 tcggttcgaa tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg
    13261 aattagggat gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg
    13321 cgtttaccga tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag
    13381 gcaaatttcg tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc
    13441 agtttatcgc caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta
    13501 tgatgtttgg actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa
    13561 ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca
    13621 tagggctatt gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa
    13681 ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc
    13741 tgtgctacag aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca
    13801 gtattctcaa atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg
    13861 ctaacctgtg tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc
    13921 agtatgtgct gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca
    13981 tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt
    14041 atttagctgg catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta
    14101 actctttcac tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca
    14161 gcctgaactg ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc
    14221 gtgccgaagg ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg
    14281 ctggcttctt gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa
    14341 gcgatgcgac attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa
    14401 ttattgctgc attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga
    14461 ttattgagga aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt
    14521 ccgtaacgtt ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta
    14581 cgtttaaaaa atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg
    14641 acctctgcct cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc
    14701 tccgatcagg gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt
    14761 atgttaacca atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa
    14821 attgaaggtt ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc
    14881 ttaattaatg ataagagtat cagttcgggt ggtgcagtgt atctgga
//
LOCUS       DAJTRW010000089        14453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRW010000089 DAJTRW010000000
VERSION     DAJTRW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(97..363)
                     /locus_tag="OO326_003089"
     CDS             complement(97..363)
                     /locus_tag="OO326_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5967390.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            637..4161
                     /gene="nifJ"
                     /locus_tag="OO326_003090"
     CDS             637..4161
                     /gene="nifJ"
                     /locus_tag="OO326_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5967391.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4528..5657
                     /gene="ompN"
                     /locus_tag="OO326_003091"
                     /pseudo
     CDS             4528..5657
                     /gene="ompN"
                     /locus_tag="OO326_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5798..6232
                     /gene="uspF"
                     /locus_tag="OO326_003092"
     CDS             5798..6232
                     /gene="uspF"
                     /locus_tag="OO326_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5967392.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6408..7343
                     /gene="ttcA"
                     /locus_tag="OO326_003093"
     CDS             6408..7343
                     /gene="ttcA"
                     /locus_tag="OO326_003093"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5967393.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7472..8839)
                     /gene="dbpA"
                     /locus_tag="OO326_003094"
     CDS             complement(7472..8839)
                     /gene="dbpA"
                     /locus_tag="OO326_003094"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5967394.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9317..10300)
                     /gene="zntB"
                     /locus_tag="OO326_003095"
     CDS             complement(9317..10300)
                     /gene="zntB"
                     /locus_tag="OO326_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5967395.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10555..11787
                     /gene="dgcM"
                     /locus_tag="OO326_003096"
                     /pseudo
     CDS             10555..11787
                     /gene="dgcM"
                     /locus_tag="OO326_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11808..12371)
                     /gene="smrA"
                     /locus_tag="OO326_003097"
                     /pseudo
     CDS             complement(11808..12371)
                     /gene="smrA"
                     /locus_tag="OO326_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12625..14079)
                     /locus_tag="OO326_003098"
     CDS             complement(12625..14079)
                     /locus_tag="OO326_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5967396.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14076..>14453)
                     /locus_tag="OO326_003099"
     CDS             complement(14076..>14453)
                     /locus_tag="OO326_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5967397.1"
                     /translation="DGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGM
                     KPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAF
                     DDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3850 a         3644 c         3413 g         3546 t
ORIGIN      
        1 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
       61 acagacatta acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
      121 ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
      181 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
      241 actactggac atcgacgctt ttaaacttgg cgctacgtgc gccgcagtcg cctgctgaac
      301 aggctcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
      361 catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
      421 gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
      481 ttttttgccc tctgtttgat caaaacattc attactctga tgtgggggac acaaaagcga
      541 aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc gcagcaattt
      601 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
      661 gcggttgctt cggtgacgtt tcgcatcagt gaagttatcg ccatctaccc tattaccccc
      721 agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg
      781 ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat
      841 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
      901 atgatcccga cgctgtacaa actggcaggc gaactgacgc catttgtcct gcatgtagcg
      961 gcacgtaccg ttgccacaca tgcactttct atttttggcg atcattccga cgttatggcg
     1021 gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg tccaggaagc gcaagacttt
     1081 gctctcattt cgcacatcgc aacgctgaag agccgcgtgc catttattca tttctttgat
     1141 ggttttcgca cgtcccacga aatcaataaa attgtcccgc tggccgatga cactattctt
     1201 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa cccggaacat
     1261 ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc
     1321 aatccctggt acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttcgctgcc
     1381 gcgacaggtc gtcagtatca gccgtttgag tattacgggc atccgcaagc ggaacgggtg
     1441 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
     1501 cgtggcgaaa aagttggcgt gctgaaagtt cgtctgtacc gtcccttctc cgccaaacac
     1561 ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa
     1621 cccggtgccc aggcagaacc gctctatctg gatgtgatga ccgcactggc agaagccttt
     1681 aataatggtg agcgcgaaac cctgccccgt gtcattggtg ggcgctatgg tctttcatcc
     1741 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaacct
     1801 aaagcacgct ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg
     1861 gaaaacactc tgccaaactc ggcgaaactt gaagccttgt tttatggcct tggtagtgat
     1921 ggcagcgttt ccgcaaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
     1981 gcacagggct attttgttta cgactccaaa aaagcgggcg gcctgacggt ttctcacctt
     2041 cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
     2101 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctagcggc
     2161 attttcctgc ttaatacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa
     2221 gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
     2281 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
     2341 ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
     2401 agttacagta gcaaaggtca ggatctggtg gagcgcaact ggcaggctct ggcgctggcg
     2461 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
     2521 ccagtggttt ccgatgccgc ccctgatttc gtgaaaaccg taaccgcggc gatgctcgcc
     2581 gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc
     2641 accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc
     2701 tgtacccaat gtaaccactg cgtcgccgct tgcccacact cagctattcg cgcaaaagtg
     2761 gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctaga tgtgaaatcg
     2821 cgtgatatgc gcgggcagaa atatgtcttg caggtggcac cggaagattg caccggttgt
     2881 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
     2941 aatatgatgt ctcgccagga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
     3001 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg
     3061 attacaccgc tgtttgaata ttcaggtgct tgccccggtt gtggcgagac gccgtatatt
     3121 aaattgctga ctcagctcta tggtgaccgg atgttgatcg ctaacgccac cggctgttct
     3181 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
     3241 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
     3301 acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
     3361 ccggcggaat tactgacggc gttgaaatca gacgctacgc cagaggttcg tcgagaacag
     3421 gtcgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact tctgcgtgac
     3481 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg ggcttacgat
     3541 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg
     3601 ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt
     3661 gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
     3721 atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag
     3781 acggtgaaag cgattcagga agcggaagcg tatccgggac catcgctgat cattgcttat
     3841 agcccgtgtg aagagcatgg ttacgatctg gctctcagcc acgaccagat gcgccaactc
     3901 actgctaccg gcttctggcc gctatatcgc tttgatccgc gtcgtgccga tgaaggcaaa
     3961 ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac
     4021 gagcagcgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa
     4081 gatgctgcag ctaatttgca aaaacgctat gacttcctag cacaaatggc cggaaaagcg
     4141 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaaagc
     4201 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
     4261 ccaacagaaa ataaaatctg tgacaataaa ggcatataac ccgcgcagaa taacgtatag
     4321 gaaattaaaa gtataatttt tattttgtat gtattatctt cgatacattc accagactta
     4381 ttatcatttc agaggaatta tttgattaag gttttactta aggcgtaaca aatgatattt
     4441 atcggctaac tgaacttctc ctttaggatg ttttcactcc ccgaaatggg gataaaaggc
     4501 aaataaaata actaaaggat ttattcaatg aaaagaaaag tactggcact tttaattcct
     4561 gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt ataataaaga cggcaacaaa
     4621 ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc agcgaaagat
     4681 ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa cgatcaactt
     4741 actggctacg gtcaatggga atacaatatt caggcaaaca acactgaatc ttcaaaaaac
     4801 cagtcatgga cccgtctggc atttgccgga ctgaaatttg cagattacgg ttctttcgat
     4861 tacggacgta attatggcgt aatgtacgac atcgaaggct ggaccgatat gctgcctgaa
     4921 tttggcggtg actcttatac caatgcagac aactttatga ctggtcgagc caatggcgtc
     4981 gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag
     5041 tatcaaggta acaacgaagg agccagtaat ggtcaggaag gcaccaataa cgaacgcgat
     5101 gttcgtcatg aaaacggtga cggctggggt ctttccacaa catatgattt aggcatgggc
     5161 tttagcgctg gtgcggcata cacctcttct gaccgcacca atgaccaggt taaccatact
     5221 gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat
     5281 atttacctag caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat
     5341 tatgcagttg cgaacaaaac ccagaacttt gaagttacta gtaccagttt gatttttgtc
     5401 tgcgtccggc agtctctttc ctgatgtcta aaggccgtga ccttcacgct gctggtggtg
     5461 cagacaaccc ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga
     5521 cttactattt caataaaaac atgtccacct atgttgacta taaaatcaac ctgttggatg
     5581 aagatgacag tttctacgct gccaacggca tctctaccga tgatattgtc gctttaggtc
     5641 tggtttatca gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct
     5701 tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg
     5761 cgttacactt tatgcggata ctaaaacagg aggttttatg aacagaacga ttcttgtccc
     5821 tatcgatatt tcagattcag aattaactca acgcgtgatt agccacgttg aggcagaggc
     5881 aaagattgat gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc
     5941 ctctctgggt ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc
     6001 caaatcgcaa ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt
     6061 ccatgttgag gaaggctcgc ccaaagaccg cattctggaa ttggcgaaga agatccccgc
     6121 tcatatgatc atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa
     6181 cgccgcagct gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg
     6241 cccgcacatt gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct
     6301 aatccgtacc atacacgcca cagtttttat atcagatttc ttatgctgag cgttccggca
     6361 tgacgcatac tcttctgatg ccatataacg aattgagtcg cttttaaatg tcgcaaaatc
     6421 aagaaattag taagaaagaa caatacaacc tgaacaaatt acaaaaacgt ctgcgtcgta
     6481 acgtgggcga agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt
     6541 gcctctccgg gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa
     6601 gcgcgccaat caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc
     6661 cggaacacgt tctgcccgag tatcttgaaa agctgggcgt tgagtacaag attgttgaag
     6721 agaacactta cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt
     6781 gttctcggct acgtcgcggt atcctttatc gcaccgcaac ggaactgggg acgacgaaga
     6841 tcgcgttggg tcaccatcgt gacgatatcc tgcaaacgtt gttcttaaat atgttctacg
     6901 gcggcaagat gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta
     6961 ttcgtccact ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt
     7021 tcccgattat tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg
     7081 ctgacatgtt gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg
     7141 cgatgcagaa tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca
     7201 ttacccacgg ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc
     7261 cactacaacc ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacggc
     7321 tgaatgtggt tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt atcgggctac
     7381 tcttatggag gccggataag acgcggccag cgtcgcatct ggcaatcccg aataagatgt
     7441 ttactcttgc acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt
     7501 ttcctttaat cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga
     7561 ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc
     7621 cgatatctcc tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc
     7681 catcgataca caacgttgcc atttctgctt ccagcgtcac aatggaacta ttagctggcg
     7741 gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt
     7801 cttccggagc acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga
     7861 tgcgatgtac atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg
     7921 atttaatatc cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt
     7981 tagcaaaacg taccagggtc tgatcgcgtt gctccagatc gccgtgtaac gacaatgcac
     8041 tttgctctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa
     8101 acaccacaca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc
     8161 ctttactgga tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa
     8221 tttcaatcgc caaaggatcg cgttgcactc gtccgctgat tgcggcgatg gcttccggcc
     8281 aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa
     8341 tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca
     8401 gcgcatccag tgataccgtg cctttttgca agtgatccag caaacgaccc ggcgttgcca
     8461 cgataatatg cggcgcatgt tgcagcgaat cacgctgcat accgaacggt tgaccaccgc
     8521 acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca
     8581 cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg
     8641 catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg
     8701 cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg
     8761 tcatggttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca
     8821 gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg
     8881 tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg
     8941 gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgccaggaat
     9001 cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg
     9061 ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa
     9121 agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat
     9181 atggagcgca acgcccatcg cttgacgttg cattcacctg caagggagat attgccctga
     9241 atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat acagacagcc
     9301 ggaaaaattg cttttgttac aaccatttac tacggtgcaa ccataaagca acaccaccaa
     9361 taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgccaggga
     9421 tcccaccaag gttgacgcca aataacccgg tcagaaaggt actgggtaaa aagaccattg
     9481 ccatcaacga cattgtatag gtacgacgag ctaaattttc ctgcatcacc tgagcgattt
     9541 catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa
     9601 ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac ggcaaacgtt
     9661 cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc atcacaatta
     9721 attgtttgcg cagcagagcc aggaatccac gcggtggaat ttgctgatca aggagattat
     9781 cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcactggaa tgatcggtca
     9841 acgcatcgca cacatccacc agccatcccc cgcaatcggt cggacccgtg ccctcttcca
     9901 gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc
     9961 gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt
    10021 ttatacagcg caatgtaatc agcgtgcctt caccgaaacg gctgactcgg ggccgtgtgc
    10081 tctcgcccgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt
    10141 gggcgctatc atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca
    10201 catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg
    10261 catccgggac attaacgtcc gatcccttaa tcgcttccac tgtgcctcca tcatcaacgc
    10321 attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct
    10381 gaaatgatta ataaaattct gtctaaattg aatacaaaaa gcaaaatgct tttccgtata
    10441 caaaccgtgt gaagtgttaa atagcgtcta tcattatcag aattatctga tcatatgacg
    10501 tggctttttt gcgatcggat agcaacaaaa attgataaaa ataacgggat ctcaatgatt
    10561 acgcacaact tcaataccct ggacttactc accagtcctg tctggattgt ttcgcccttt
    10621 gaggaacagt taatttatgc caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt
    10681 agtcagctac gaaccggacc ctattccgtc tcctcacaaa aagaactgcc gaaatacctc
    10741 tccgatctgc aaaaccaaca cgatattatc gaaatcctca ctgttcagcg taaagaagag
    10801 gaaacagcat taagctgtcg gcttgttttg cgagagctga cagaaacaga accggtgatt
    10861 attttcgaag gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg ctcggcaaat
    10921 tatcagcgca aaaaacaagg tttttatgcg cgcttttttc tgactaactc tgcaccaatg
    10981 ttgttgattg acccgtcacg agatggacaa atcgtcgatg ctaacctcgc cgcgctcaat
    11041 ttctatggtt ataaccatga aacgatgtgc cagaaacata cctaggaaat aaatatgctt
    11101 gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt tacccggcgg tcataaccct
    11161 ttgaattttg ttcataaact ggcggatgga tctactcgtc atgtgcagac ctatgccgga
    11221 ccgattgaaa tttatggcga caagctcatg ttatgtattg tgcatgatat tactgagcaa
    11281 aaacggctgg aggagcagct ggaacatgct gctcaccatg acgcgatgac cggattactg
    11341 aatcggcgac agttttatca cattacggaa ccaggccaaa tgcagcatct cgccatcgct
    11401 caggattaca gcttgttgct catcgacacc gatcgtttta aacacattaa cgatctctat
    11461 gggcattcta aaggtgatga ggtgttatgc gccctcgccc gccccctcga aagttgcgct
    11521 cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga
    11581 accccactgg ataccgcgct ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg
    11641 agtatttcgg gcttaccacg ctttaccgtc agcattggtg tggcgcatca cgaaggaaat
    11701 gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat
    11761 ggacgcaacc gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca acgactgcgc
    11821 ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa
    11881 cacgccccgc tgccgccatg atgcggtagc gcggtgcaat atgcctgaac atcatcaaat
    11941 tcggtcagcc agcgcgccac atagctgcgg acaatattgg catgtgattt atcttcccgc
    12001 cctttaccat gaataatcag cacattacgc aaaccatccg ccagggcttg ttgaatgaaa
    12061 ctgaacacca ttttgcggca ctcttccacc ggctggcgca aaagattcag gctcgcctgt
    12121 tgcggatatt taccactgcg cagcttatcc agcaccccat gctacaaccc ttcctgccga
    12181 aactccagcg gctgacttag cgggatgatg tcgagaaatc cggtagtgag gaaattatca
    12241 agctgcagcg tgtcgatacg ctgcggcgca cgttggttac gcgttggatg ccagtggaca
    12301 tctgtagcac gtttcagcgg ctggacatct tccatggcgt caagaaacag cgatttgtcg
    12361 tcaaggttca tgtaacctcc tatgcacctt cacacgtcgt gattcagacg ggttaattgt
    12421 tcatacttac tgcatcgttc ccttcacaga ttgaaacaat actctacttc atacctgtga
    12481 gtatctgcgc aggcggggca ttttatacta gcctgttagc agtatcgaac agtttagccc
    12541 gagtttaatt cagcacaggc tccgatcagg atgatttatt catagcagga accaacaaaa
    12601 atgcgataaa taacagacca ctgattagca gaaaggtacc gaaaatgcca tagtttgcgc
    12661 ctccctcagc atcaattgac tcaataattt ggttgaagcc agtatttccg atgtctggtc
    12721 acttctactg cgattaaaaa ataaagcact tctggaaatg aagtcattca ccgtatgggg
    12781 aacagaaacc attttttcac ccattctcga aaaatgaaaa ttagttcgat tattgagaat
    12841 agagccacac agggcaatac caatcgcgcc cccaagatta cgtgttaaat taaatacacc
    12901 agaggctaac ttcagccgtt cttttggaat tccccccagc gttaatgtta cgatgggggc
    12961 catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga aacagtaatt cctgccatcc
    13021 ccattcgtgg gttatagggg taaaaagata cattgaaaac acaaatccgc ccagccccgc
    13081 catcaacagc catcgcagat ttattttttt acttaaccag aagtaaaagg gaacagaaaa
    13141 taactggaaa atacctgttg tgcaaacggc aaaacctatc tcttcggcat ttaatccgcg
    13201 aatctgcccc aaaaacacag gtattaaata tacagtagag aatatcccaa cccctccaga
    13261 aaaagaaaaa aaacatccca gagtgaaatt tttatcctta aaagcatgaa gatccattat
    13321 cggattggat attgtaagtg tacgcgcagc aaataaaata aaactcacca atgccagaac
    13381 tgatgtcagc aaaatagtat tatcatccag ccatccccag cgcgctcctt cttcaagggt
    13441 atattccaga catcccagag tcatggccaa caggataata ctcggataat cagcaacttt
    13501 cagtaaagag agatcaggtt tatcaaaatt aaccagaaaa ggaatactca aaactaaata
    13561 tatgccaggt aatatattga tataaaatag ccatcgccag tcgaggttat cagtgagcca
    13621 gccccccaga gttggcccta gcgtcggtga cagtgaggcc agcgcactga ctaccgcagc
    13681 agccaacccc aactctttac cctggtaata aataaacgcc atcgtgaaaa ctaaagggat
    13741 catggatgcg ccagcagctc cctgtaaagc acgaaacaaa atcatgatct gaatattcca
    13801 tgcaagtcca cacgcgatac tcattaaagt aaatattccc gcggatagag taaaaagcca
    13861 acgagtggaa aatacccgtg acagccaccc agagagcgga ataacaataa tctcagcaat
    13921 taaatagctg gtctgaagcc aggccatttc gtctttacct gcggacagac caccacctat
    13981 ttcatttagg gatgacgata caatttgaat atcaagctga gcaataaata atcccataca
    14041 catactggcg aaagcaatat gtttacgcat agacatcatc tctcatcaac actgacggtg
    14101 acggacagtc ccgggcgaag aagttgcttt aattcctttg cgtcgtcaaa tgcaatccgt
    14161 accgggacac gctgaacaat tttggtgaaa ttgccagtgg cattttctat cggaatcaag
    14221 ctgaaactgg ctcctgtcgc tggggaaagg ctttcaatgt gaccgtgaaa cacttcccct
    14281 ttgagaatat cagcacggat ctcagccttc attcctggct tcattcccgc tatttggttt
    14341 tctttataat ttgcatcaac ccacaactca ctgactggta ctagtgaaac taacgatgtg
    14401 ccgccttcca cccaactgcc tgtatgggcg ctgcggtttg cgacaatgcc atc
//
LOCUS       DAJTRW010000090        14416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRW010000090 DAJTRW010000000
VERSION     DAJTRW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..122)
                     /locus_tag="OO326_003100"
     CDS             complement(<1..122)
                     /locus_tag="OO326_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5967398.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHA"
     gene            305..448
                     /locus_tag="OO326_003101"
     CDS             305..448
                     /locus_tag="OO326_003101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967399.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(667..2205)
                     /gene="aldB"
                     /locus_tag="OO326_003102"
     CDS             complement(667..2205)
                     /gene="aldB"
                     /locus_tag="OO326_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5967400.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2370..3521)
                     /gene="yiaY"
                     /locus_tag="OO326_003103"
     CDS             complement(2370..3521)
                     /gene="yiaY"
                     /locus_tag="OO326_003103"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5967401.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3711..5555)
                     /gene="selB"
                     /locus_tag="OO326_003104"
     CDS             complement(3711..5555)
                     /gene="selB"
                     /locus_tag="OO326_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5967402.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5552..6943)
                     /gene="selA"
                     /locus_tag="OO326_003105"
     CDS             complement(5552..6943)
                     /gene="selA"
                     /locus_tag="OO326_003105"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5967403.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7041..7649)
                     /locus_tag="OO326_003106"
     CDS             complement(7041..7649)
                     /locus_tag="OO326_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5967404.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7720..8805)
                     /locus_tag="OO326_003107"
     CDS             complement(7720..8805)
                     /locus_tag="OO326_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5967405.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8808..9170)
                     /locus_tag="OO326_003108"
     CDS             complement(8808..9170)
                     /locus_tag="OO326_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5967406.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9707..11620
                     /gene="mtlA"
                     /locus_tag="OO326_003109"
     CDS             9707..11620
                     /gene="mtlA"
                     /locus_tag="OO326_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5967407.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11715..12863
                     /gene="mtlD"
                     /locus_tag="OO326_003110"
     CDS             11715..12863
                     /gene="mtlD"
                     /locus_tag="OO326_003110"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5967408.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12863..13450
                     /gene="mtlR"
                     /locus_tag="OO326_003111"
     CDS             12863..13450
                     /gene="mtlR"
                     /locus_tag="OO326_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5967409.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13462..13671)
                     /locus_tag="OO326_003112"
     CDS             complement(13462..13671)
                     /locus_tag="OO326_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967410.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13955..14317
                     /locus_tag="OO326_003113"
     CDS             13955..14317
                     /locus_tag="OO326_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5967411.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3525 a         3841 c         3620 g         3430 t
ORIGIN      
        1 gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa aataataatc
       61 ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata gtccaggaac
      121 atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa tgccatgatg
      181 cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt ctgaaatgtg
      241 ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac tattggccaa
      301 actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta aaccttcaat
      361 tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg gcctggttcg
      421 cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca gaaacgcact
      481 tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt gtaacaacgt
      541 gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt caggttcccg
      601 taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg cgaccagctt
      661 cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc acttggtttg
      721 ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt gtttgtagcc
      781 accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc gcccagcctg
      841 tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc ccgcgcccag
      901 gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga aggtggtcac
      961 cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct gaccaaacag
     1021 aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct tgcgccgccc
     1081 gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga ggatggtttc
     1141 cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg ggttaccgct
     1201 gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga ttgactcctg
     1261 cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa acagtgcaaa
     1321 gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag caaagaagat
     1381 atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg ttgcgtattg
     1441 cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga tgcgtttcga
     1501 ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca ccacgcccgg
     1561 cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg taagacgtgc
     1621 gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc agctcgccat
     1681 cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt catggaaatg
     1741 ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc gaatacacga
     1801 ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg tttcgcgaat
     1861 gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt gttccattcg
     1921 atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg cccatttatc
     1981 tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg aagacgccac
     2041 ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac catcggcagg
     2101 ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga tggggaaacc
     2161 atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg tctccttgcg
     2221 gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg gtaacagctt
     2281 tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa aagcggctga
     2341 caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat caccacaatt
     2401 tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag ggcattagtc
     2461 gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc cgggatatcc
     2521 actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc cgcgtcgttt
     2581 ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc agcggcgact
     2641 ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc gtgtggcagg
     2701 ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga agcattattg
     2761 aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt cgcattactg
     2821 ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt cagtgcacaa
     2881 gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg cgttaaggca
     2941 tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga ggagtcattg
     3001 acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg acgcgcttcg
     3061 tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt attgatggcg
     3121 atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg aatatcgccg
     3181 ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc accgcctaag
     3241 gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc gacgttttcc
     3301 gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg ttcttccagt
     3361 gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt gacaattaag
     3421 gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga atcagcgccg
     3481 atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc ctgaaagtgt
     3541 aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat tattattaat
     3601 tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc tttatttaaa
     3661 atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc ttatttttcc
     3721 ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt aaagccaatg
     3781 cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa gcgatcgcgg
     3841 aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat attggcaaat
     3901 tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat tattccttgc
     3961 tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt ctcttttgcc
     4021 agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg ccagatggcc
     4081 tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc gtgatgactg
     4141 aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc ttcatcttcc
     4201 attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc gcgatgttgc
     4261 tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc aaccggcgca
     4321 ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc gcgcatccct
     4381 tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc gcgttgcagg
     4441 tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag ccattgcaga
     4501 tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg cgctccggcc
     4561 agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc cagccataac
     4621 ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg tcccgtgacg
     4681 tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc atgggtttga
     4741 agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag ccagtcgcca
     4801 cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg ctgcccggca
     4861 ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat cggtttattt
     4921 acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc cgtaccggtg
     4981 acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg gaaactatgt
     5041 tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc atcaattccc
     5101 cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta ttcccgcaga
     5161 acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg atcggctttg
     5221 gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag atgctcacgg
     5281 gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc gataccacca
     5341 acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat aaaaccaggc
     5401 acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat gccgcgcttt
     5461 ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa ggttgttttg
     5521 ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc aaaaaccgtt
     5581 gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata cgaccaatca
     5641 ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta ccgcgtccat
     5701 catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc gaaccactgc
     5761 caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa tgtgcgacaa
     5821 ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg gtaagcaggc
     5881 gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc aacgtggctt
     5941 ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg tggctttgca
     6001 ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg cctaacaact
     6061 tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc ggcattggct
     6121 ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa tcagtcacca
     6181 ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc gctttggtga
     6241 acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta ttttcattca
     6301 ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg tgtagggtgc
     6361 agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc accagttcgc
     6421 cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat aacaccgccg
     6481 ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac agcagctgcg
     6541 ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg agggtcactg
     6601 gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa gctcgcccaa
     6661 ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg ctctgcgcct
     6721 ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca ggcagcgtct
     6781 ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat tccaccacgc
     6841 gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat aagcgatcaa
     6901 tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac ctggctgatc
     6961 aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac gtgagatggg
     7021 tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa actttcgcgg
     7081 ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca acctggcgca
     7141 acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc cagattgacc
     7201 gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag actgcggttg
     7261 attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg acgcgcctgt
     7321 tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc aattttgcgc
     7381 acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt cattaactca
     7441 atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt caccagcacc
     7501 ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata gggcagttca
     7561 ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt gcgtacaaac
     7621 gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga aaaatatcag
     7681 tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca aataaagata
     7741 atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat ggctgaaatg
     7801 gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt
     7861 agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg ttaatgattg
     7921 caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac tagtcaattt
     7981 gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat cgccaggttt
     8041 cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt gctcggggat
     8101 gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc ggatcagtac
     8161 ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat tatattttgc
     8221 ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga ccatactgtc
     8281 gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg catcctgcgc
     8341 gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca ccgccgcctg
     8401 gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg ctgaaacctg
     8461 ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat tatgcgtcgc
     8521 cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg ggtcgagctt
     8581 aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt cagtaacaat
     8641 tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg taaaagtgta
     8701 agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc ctcccagcgc
     8761 cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta gatctccggg
     8821 aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg ggaaattcgg
     8881 tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac atgtgactgc
     8941 ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa cggccagata
     9001 acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc atgggggtga
     9061 ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc
     9121 accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg
     9181 gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca ggctgatatt
     9241 ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg
     9301 atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg tagatcacat
     9361 caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc
     9421 aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa tcaccgcctt
     9481 gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc tctaccccct
     9541 atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac cgattcagtg
     9601 ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct
     9661 cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat
     9721 taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg
     9781 cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa
     9841 cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc tgctgatcgg
     9901 ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg ccattaccac
     9961 catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc
    10021 gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg
    10081 ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct
    10141 ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt
    10201 taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa
    10261 aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca
    10321 gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat
    10381 gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg
    10441 tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat
    10501 gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac
    10561 tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc
    10621 gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt
    10681 ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat
    10741 tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct
    10801 atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga
    10861 cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc
    10921 aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga
    10981 cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcaca
    11041 gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct
    11101 cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag
    11161 ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg
    11221 caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg
    11281 ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta
    11341 tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa
    11401 aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga
    11461 cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac
    11521 cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag
    11581 cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta ccctctcctc
    11641 acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga
    11701 tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta
    11761 tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg
    11821 tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt gaaaccgagc
    11881 aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc
    11941 tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg gaacgtattg
    12001 ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa tccccgctga
    12061 acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt catgtgatga
    12121 acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt gtcgattccg
    12181 ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg gaagtgacgg
    12241 tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca ctgccgaaca
    12301 tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc
    12361 tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc
    12421 gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg
    12481 gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg tacatccaga
    12541 aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc
    12601 agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc ggtacgctgg
    12661 aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg cacttccgca
    12721 gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg
    12781 cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa
    12841 ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa
    12901 cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa accgtgtgct
    12961 tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctttt
    13021 gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa
    13081 gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt
    13141 aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggaactgct
    13201 gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta ccgacgacga
    13261 aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc cgccacagtt
    13321 tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg
    13381 atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa aaaaagcctt
    13441 tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat aaagccgacg
    13501 ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc cggacggaat
    13561 cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg atgccatgaa
    13621 atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca tgatgatatc
    13681 tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa
    13741 gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt ttattatctt
    13801 tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg ttaatgatta
    13861 cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg cgcatactag
    13921 gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa aaaacgaaat
    13981 caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc tgtcgctggt
    14041 tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg aagcagaaat
    14101 tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga agctgatgaa
    14161 gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca agctgaagaa
    14221 aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg aaatgggcct
    14281 ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga ctggttcgat
    14341 cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc ctgtctactc
    14401 gcctaattac caaaca
//
LOCUS       DAJTRW010000091        14314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRW010000091 DAJTRW010000000
VERSION     DAJTRW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..3049
                     /locus_tag="OO326_003114"
                     /pseudo
     CDS             1..3049
                     /locus_tag="OO326_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3059..6705
                     /locus_tag="OO326_003115"
                     /pseudo
     CDS             3059..6705
                     /locus_tag="OO326_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6766..7173
                     /locus_tag="OO326_003116"
     CDS             6766..7173
                     /locus_tag="OO326_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967412.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7715..8803
                     /locus_tag="OO326_003117"
     CDS             7715..8803
                     /locus_tag="OO326_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5967413.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8814..11093
                     /locus_tag="OO326_003118"
     CDS             8814..11093
                     /locus_tag="OO326_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967414.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11086..12222
                     /locus_tag="OO326_003119"
     CDS             11086..12222
                     /locus_tag="OO326_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5967415.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12219..14222
                     /locus_tag="OO326_003120"
     CDS             12219..14222
                     /locus_tag="OO326_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967416.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3545 a         3755 c         3653 g         3361 t
ORIGIN      
        1 gattatgcga ataatgtcat tattattctg ccgtctggca ttgcacctga ctacttggag
       61 caatattcca gatttgaatt acgcctacgt aaacatttat cactggcgga agagtctctc
      121 tggcaaaaat gtgcacaaaa acttattgcc gcaattccac atattccaga atggcggcaa
      181 ccattaattg ctttgttatt acccgaaaaa ccagaaattg cacatgaaat tgcccagcat
      241 ctactggggc aaaaaaaatt accctcgctt gagtggttaa aaatagtggc gactgatgag
      301 cacattcttg cctcattaga aaaatatcac gagccatatg ccatttttga tgattactat
      361 tgtggtgcga tatggtcagc caccgtatta caggagcaag gtgttgcagc cctgccccga
      421 tttgctccct atgccgcaag tgactactgc gccgatgtgt tgcgtcatat caatcatccg
      481 ttcgcattaa cactgcttat acgtgtagcc gggcatacta aacgctgtca cgatcgaatg
      541 acgaaagcct gcgctgcgtt cccacacgca gcactggcag cactggcgga acttctagtg
      601 caaaaagaag agaatagttg gcgcattatg ctaatgacga tgtttatctc gcaaccagca
      661 ctggcagaac aggtcattcc ctggctttcg acacccgcag ttgcagtgct gaaatcatgc
      721 cagcaccaac tgacacagcc ctcaaaccat gccagcgccg atctactgcc agccatagta
      781 gtctccccac catggatttc gaaaaagaaa aaatcgccga ttccggtgct ggatttagcg
      841 ccattaggca tcgagccaat ctgttatctg acagaagaaa tcagtaatca acttttggcg
      901 aaatatatct ggtattcaaa acacatcacg gttagccatg aagaaagtac taccaacctg
      961 ttggcaagga tgggttttca acgacggatc gctggtacat atattaaagc tcccgaagcg
     1021 gtagttgagg catggctaaa tgaagattat tcaaccttac taagtgaatt taaggtgttt
     1081 cattcaccta ccgggcatta ttggcagttg gggattttga caacattgcc gctggaggct
     1141 ggagaaagca gtaaaagcat ggaatgccct tactctatct ccacataccg ataccgaata
     1201 cgccatgtta cattttggac tcaaaggttt acctggttta gttaactcaa ttacacgcta
     1261 tccacaggaa gccttgccca tcacgaatta cttcgcagcg agtgagctgg ctcctgccgt
     1321 cgcccgtgcc ttcaacaaac tcaaaacgct acgcgaaaac gcccgcacct ggctgttgaa
     1381 atacccggaa catgccctta ccggcctgct gcctgcggcg ctcggcaaag ccggtgaagc
     1441 acaggataac gcccgcgctg ccttgcgtat gcttaccgaa aacggtcatc agccattact
     1501 gcaagaaatc gcccggcgtt ataaccagtc ggaagtaaca gacgcggtga acgctctgct
     1561 tgcgctcgat cccttagata atcacccgac aaaaatcccc actcttccga ccttttatca
     1621 gccatcgatc tggacgcgcc cggtattaaa agcaaacgcc caatcactgc cagataacgc
     1681 cctcctccac ctcggcgaaa tgctccgctt ccctcaggaa gaggctctgt atccgggatt
     1741 attgcaggtg aaagacgcct gtaccgctga ctctctggct gaatttgcct gggatctgtt
     1801 taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca ctgcgttagg
     1861 cgttctcggt aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg cctggcctgg
     1921 tgaatcccag cataaacgcg ccaccgttgg gttggatatt ctcgccgcta tcggtagtga
     1981 tatcgccctg atgcagctta acggcattgc tcagaaactg aaattcaaag cattacagga
     2041 gcgggcaaaa gaaaagattg ccgacattgc cgagagccgc gaactcacgg tagcggagct
     2101 tgaagatcgg ttagcaccgg atctcggtct ggatgataac ggttcgttgc tgctggattt
     2161 tggcccacgc cagttcaccg tcagctttga tgaaacctta aaaccgtttg tgcgtgatgt
     2221 ttccggcagc cgcctgaaag acctgccaaa accaaacaaa agcgattatg agtcacaggc
     2281 gaacgatgct gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg tcgcggccca
     2341 gcaggtagca aggcttgaat ccgccatgtg cctgcgtcgc cgctggtcgc cggaaaactt
     2401 ccaactcttc ctggttgtgc atccgctggt tcgccactta acccgccgtc tgatttgggg
     2461 cgtttatagc gccgacaacc agctactggc ctgtttccgg gtagcggaag ataacagcta
     2521 cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta tcggcattcc
     2581 tcacgttctg gaaatatcac cgacggatgc tgtcgccttt ggtcagcttt ttgccgacta
     2641 cgaactgcta ccaccgttcc gtcagcttga ccgtaacagt tacgccctga cagaagccga
     2701 gcgcaacgcc agcgaactga cccgctgggc aggcagaaaa tgcccgagcg gccgggtcat
     2761 ggggctggca aataaaggct ggataaaggg cgaaccacag gatggaggct ggatgatcaa
     2821 acctctcggt cgctggtcgt taatcatgga aatcgacgaa ggttttgcgg tgggtatatc
     2881 accagccgaa cttagcgctg agcagctctt aagcaagctg tggctatggg aaggcaaagc
     2941 ggaaagctat ggctggggga gtaattcaac acaggaagcg cagttctccg tactcgatgc
     3001 catcaccgcc agcgagctaa ttaacgatat tgaagccctg tttgaataag gaaagagcat
     3061 ggacaaggaa ttaccgtggc tggcggataa cgcccaactg gaactgaaat ataaaaaagg
     3121 caaaacacca ctcagtcatc gcaattggcc gggcgaacca gtgcctgtta tcactgaaag
     3181 catcattcag acactgggtg atgaattgct acaaaaagct gagaagaaaa aaaacattgt
     3241 ctggcgttat gaaaattttt caccggagtg gcagtccgcc attacgcagg ccatcaactt
     3301 gatcggcgaa cacaaaccct cagtcccggc ccgcacaatg gcggcactag cctttatcgc
     3361 gcaaaatgac aaccaacagt tgctcgacga aatcgtccaa caagaggggc tggaatatgc
     3421 gactgaggtg gtgattgcac gccagtttat tgcacggtgt tatgagagtg atcctctggt
     3481 agtcacattg cagtatcaga acgaggacta tggctatggt tatcgctcag aaacctataa
     3541 cgaattcgat ctccgactgc gtaaacacct ctctctggca gaggaaagct gctggcagcg
     3601 ttgcgccgac aaactcattg ccgcactgcc aggaataacc aaagttcgcc gcccttttat
     3661 tgcgctgatc ctccccgaaa aaccagaaat cgccaatgag ttggtaagcc ttgaatgccc
     3721 gcggactcat tttcattcta aggagtggtt aaaagttgtt gctactgacc ccaaagcggt
     3781 gagaaaactc gaacgctact ggagccagga tatatttagc gatcgagaag ccagctacat
     3841 gtcgcatgaa aaccacttcg gctacgcggc ctgcgctgcc cttttgcgcg aacaaggact
     3901 ggcagtcgtt ccgcgcctcg cgatatatgc ccataaagaa gattgcggca gtctgctggt
     3961 acaaattaac catccgcaag tcatccgcac cttgctgtta gttgctgata aaaacaaacc
     4021 cagcctgcaa cgtgtggcta aatacagtaa aaacttcccc catgcgacac tcgccgcact
     4081 ggcagaactg ctggcgttaa aagaaccacc agcccgccca ggttatccaa tcatcgaaga
     4141 caaaaagctg cctgcacagc aaaaagctcg agatgaatac tggcgtacgc tgttacaaac
     4201 gctgatggca tcgcagccac aactggcagc agaagtgatg ccgtggttaa gtactcaagc
     4261 ccgggcagtg ctgaatagtt atttatcggc accgcccaaa ccagttattg atagcaccga
     4321 taacagccaa atgcctgaaa tcctcgtttc accaccatgg cgtgggagaa agaaaaagac
     4381 ttccatacgt cacttttcgc tgcctgatct tacgcttgca ccgcaaacgt acaggtcgcc
     4441 gaataaacac tggacgccgg aacagcaaac tgcaattgac cattttagcg ccatgtcttt
     4501 tgacgaacgt cttgctacga gaggagcgga tatcttcttg cgggaattag gttttgacgt
     4561 tcatagcctg gaatttaata agcgtcttct taatggacag ttggatgcct ttgatgctca
     4621 gcaactgcaa gaatacacat caataggact attgcgtact tatcacgcgc atcaggccaa
     4681 atctaaacag taccgggaag acgccaccaa tgcattactg gcacaagata gcattgcgtt
     4741 gatgggaggg tggcgaactt ttcagcacgg attttactat ggtaatgaac agcgttgggg
     4801 gatttggaat ctgtacctta tcgcgcagat gccgcgcgaa atggcggtat cgtgctggca
     4861 acaaattgtc gaggctgatt ttcattacac cggggtggaa tatttgttaa gtgtgctggg
     4921 taccgatgct ttgcctggat taaacaccgc ctttgcccga catccaaaag agatatttcc
     4981 gctgctgatt catttcggcg caacagaact ggccctgccc attgcccgcg tctggcacct
     5041 ttttgcgggc cagcgtaatc tggctcgcca gtggatttta caatggccgg aacatacggc
     5101 tactgcactt atccctcttg tcttcaccaa atctagcgat aaaagcgaag ctgcattact
     5161 tgccctgcgt ttactttacg aacatgggca tggcgaatgg ctacaaactg tagccaatcg
     5221 ttggcagcgt acagatctat ggcctgccct ggaacaatta cttaaacaga gtccaataga
     5281 gatttacccg gcacgcattc caaaagcccc tgatttctgg catccgcaaa tgtggtccag
     5341 gccgcgcctt atcactaata atcaacctgt taccgatgac gctctggaaa ttatcggcga
     5401 aatgctgcgc tttacccagg ggggacgttt ttatagcggg ctggaacaac tgaaaacgtt
     5461 ctgccagcca caaacgctgg cagcttttgc ctgggatctc ttcactgcgt ggcaacaagc
     5521 tggtgccccc gcaaaagaca actgggcatt tctggcgtta agtctctttg gtgacgaaag
     5581 cacggcacgg gatctaacga cacatatcct cgcctggcca caagaaggca aatctgcccg
     5641 tgcggtcagt ggcatgaaca tccttaccct gatgaataat gatatggcgc tgatgcagct
     5701 gcatcatata tcgcaacggg cgaaatcccg ccccttacgt gataacgcgg cggaatttct
     5761 tcaggtagtc gcagaaaatc gcgggctaag ccaggaagag ttagcggaca gattagtccc
     5821 aacactgggc cttaatgatc cgcaggcgtt gagttttgat tttggtcccc ggcagtttac
     5881 cgttcgcttc gatgaaaacc tcaacccggt tatctttgat cagcaaaacg ttcgccagaa
     5941 aagtgttccc cggttgcgcg ccgatgacga tcaactgaaa gcgcccgagg cactggcccg
     6001 actaaaaggg ttaaaaaaag atgctactca ggtgagcaaa aacctgctcc cgcgtcttga
     6061 agctgcccta cgtaccaccc gacgctggtc gctggcagat tttcattctc tgtttgttaa
     6121 tcatcccttt acccgtctgg ttacccagcg attaatatgg ggggttatcc ggcaaatgaa
     6181 ccgcgtcgtt tactcaacgc ctttcgtgtg gccgcagagg gggagttctg caatgcgcaa
     6241 gatgagccaa ttgacctgcc tgcggacgct ctgattggca ttgcccaccc gttagaaatg
     6301 acagcagaaa tgcgcagtga atttgcacag ctttttgccg attacgaaat tatgccgcct
     6361 tttcgccagt tgtcgcgccg cacggtgctg ctcacacctg acgagtcaac cagtaacagc
     6421 ctgactcgct gggaaggtaa atccgctacc gttgggcaac ttatgggaat gcgatacaaa
     6481 ggctgggagt caggttatga ggacgcattt gtctatgacc tgggtgagta ccggctggtc
     6541 cttaagtttt cacccggttt taaccactac aatgttgcta gcaaagcgct aatgagcttc
     6601 cgttctcttc gagtgtaccg tgacaataaa tccgtcactt ttgccgaact tgatgtgttt
     6661 gatttgagtg aggcgttaag cgcacctgac gtcattttcc attaaaacaa agcgggcagt
     6721 gatgcgactg cccgttatct acacgactta ccagcgggga aagcgatgat cgtgcaaaaa
     6781 gagctggttg ctatttacga ttatgaggtc cctgtacctg aagatccgtt ttccttcaga
     6841 cttgagatcc ataaatgctc tgaattattt acaggttccg tctaccgact ggaacggttc
     6901 cggctacgtc caacatttca tcaacgtgat cgagaggatg ttgacccgct aataaatgat
     6961 gcgttgattt atataagaga tgagtttatt gatgagcaaa aattacgagg cgaatcacct
     7021 gaacctgtaa tagtaatttt taattgtaaa ctacagaata tattcaacca acaaatagaa
     7081 ttatatactc tagataattc aagtcgcagc acatgcaact tgaagtatga cgagtatagc
     7141 tttattaaag gggggaacgg tattccccct tagatgcgtt tcaatttgcc cgttacttca
     7201 acaaatcaat caaccgtaac agagccacta atagctccag tacagcaatg agcacggtta
     7261 acttttgtgt cagcgtgtcc atccgctctc cttattaagg agcacaacac cagcacctta
     7321 cccttacact gcctgtcgat actggtttac gtttgttgag tcgccatgtt gcactccgga
     7381 tcagagcact acctgcggca ccacccgtcc ggtagccagg acttgaaata ggttcgatcc
     7441 ggcgcaactt cccaacagtc aggattataa atctcattat cttaattgca acactgtcca
     7501 gaggtggtta taattcgttc gttggaacac gatgttgcac aggcagtggt gttaggcctg
     7561 aaaatagtaa ataaaagaac ctgcctcacc agcagttttt tttatttact gtgatctgct
     7621 ttccagatat ttttcgctca aacaactaat gcgccaaaca tttattgcgc gtaaaatatc
     7681 gtttatttca ttaatacatt tcagggatga atatatgtca ccgcagaata atcatctgca
     7741 gcgtccgcct gctgctgtgt tatacgccga tgaactggca aaattaaaac aaaatgataa
     7801 cgcaccttgc ccgcccggtt ggcagttaag tttgcctgcg gcccgtgctt ttatccttgg
     7861 cgatagcgcg caaaatatta gccgtaaagt ggtgattagt ccctccgctg tcgaacgtat
     7921 gttagtcact cttgccaccg gacgcggttt gatgttggtg ggggaacctg gcaccgcaaa
     7981 atctctcctg tctgaattac tggcaacctc tattagcggc gatgccgggt taaccatcca
     8041 gggcggcgca tctactaccg aagatcaaat caagtacggc tggaactacg ccctgcttat
     8101 caatcatggt ccatcaacgg aagcgctggt ccctgccccg ctttaccagg gaatgcgcga
     8161 cggcaaaatc gtccgttttg aagagataac gcgtacaccg ctggaagtgc aagactgtct
     8221 gttaggtatg ctttctgaca gggtgatgac ggtgccggaa ctcactggtg aagccagtca
     8281 gctctatgcg cgagaaggct ttaatattat tgccactgcc aatacccgcg atcgcggcgt
     8341 caatgaaatg agtgctgcgc taaagcgtcg cttcgatttt gaaactgtct ttccgattat
     8401 ggatttcgcc caggagctgg agcttgtcgc cagtgcttcg gcgcgtttgc tggcccatag
     8461 cggtattccg cataaagtgc ctgacgcggt actggagtta ctggtccgca ccttccgcga
     8521 tctgcgtgcc aacggcgaaa agaaaacgtc aatggataca ctaacagcga ttatgtccac
     8581 cgctgaagcc gtgaacgtgg cccacgccgt aggcgtccgc gcctggtttt tagcgaatcg
     8641 cgcaggtgag ccagccgacc tggtggactg cattgccgga accatcgtca aagataacga
     8701 agaagatcgc gctcgtctgc gccgttactt cgaacagcgt gtcgccacac ataaagaagc
     8761 tcactggcag gcttattatc aagcccgcca ccgcctgccg tgaggaaaga tgcatgagcg
     8821 agccgttaat tgtcggcatc cggcatcata gtccggcctg cgcccggctg gtgaaatcgt
     8881 taatcgaaag ccagcggcca cgatacgtgt tgattgaagg ccctgctgat tttaatgacc
     8941 gggtagacga actgttttta gcccaccagc ttccggtagc tatttacagt tattgccagt
     9001 atcaggacgg tgcagccccc gggcgtggtg cctggacgcc atttgctgaa ttttcgccgg
     9061 agtggcaggc gctacaagcc gatcgtcgta ttcaggcaca aacttacttc atcgatttgc
     9121 cttgctgggc gcagagtgaa gaagaggacg attcgcctga tacgcaagag gaaagccaga
     9181 ccttgctgct gcgtgccacc cgcatggata acagcgacac cctgtgggat cacttgttcg
     9241 aagatgaaag ccagcaaact gcattaccct ctgcactggc gcactatttt gcccaactgc
     9301 ggggcgactc ccccggcgat gctctaaatc gtcagcgcga agcctttatg gcccgctgga
     9361 ttggatgggc gatgcagcaa aataatggcg acgtgttagt tgtctgcggt ggctggcacg
     9421 ctccggcact ggcaaaaatg tggcgcgaat gtccgcaggt aattaacacg ccagaattgc
     9481 cgtcgctggc agatgccgtt acaggttgtt atctcacccc ctacagtgaa aagcgccttg
     9541 atgtgctggc aggatacctt tcaggaatgc ctgccccggt ctggcaaaac tggtgctggc
     9601 agtggggctt acagcaggcg ggagaacaac tgctaaaaac ggttctcacc cgtttgcgcc
     9661 agcacaagtt gcctgcttcg accgcggata tggctgccgc tcatctgcat gcgatggcac
     9721 tggcacagtt gcgcggtcat acactaccgt tacgcactga ctggctggat gccatagcag
     9781 gatcgctgat taaagaagcc ctgaatgcgc cgttgccgtg gagctatcgc ggcgttattc
     9841 atcccgatac cgatccgatt ctgctaacgt tgatagacac attagcgggt gacggattcg
     9901 gtaaacttgc cccttctacg ccacaaccgc ctctgccaaa agatgtcact tgcgaactgg
     9961 aacgtaccgc aatctctctt ccggcggagc ttaccttaaa tcgctttaac cccaatggac
    10021 tagcgcaaag tcaggtgtta catcggctgg caatactgga gatcccaggg attgtacgcc
    10081 agcagggaag cacactgaca cttgcaggca acggtgaaga acgctggaaa ttaacccgcc
    10141 cgcttagcca gcatgcggca ttgattgagg ccgcctgctt tggtgccaca ctcctggaag
    10201 ccgcacgcca taaattagaa gccgatatgc tggacgcggg tggaatcggc agcattacca
    10261 catgtcttag ccaagcggcg tcagcgggtc tggcgtcctt cagtcaacaa ttactggagc
    10321 aactcacatt attaatcgcc caggaaaatc aatttgccga aatgggccag gcgctggaag
    10381 tgctatatgc cttatggcgg ctggatgaaa ttagcggtat gcaaggcgcg cagatattac
    10441 aaacgacgtt atgcgcggct atcgatcgca cgctgtggct gtgtgaatcc aacggcagac
    10501 cggatgaaaa ggagtttcac gctcacttgc atagctggca agcgttgtgt catatcctgc
    10561 gcgatctaca tagcggtgtg caactccccg gcgtttcgct ttctgcggcg gtagccttgc
    10621 tggagcgaca cagtcaggcg attcatgtcc tggcactgga tcgcggcgcg actcttggtg
    10681 cgctgatgcg tctggaacat cccaacgcta gtgccgaagc ggcgctgacg atgctggcgc
    10741 agttatcccc ggcacaatcc ggcgaggcgc tgcacggttt gctggcatta gcccgtcatc
    10801 aactggcctg tcagccggca tttatcgccg gtttcagcag tcatttaaat caactaagtg
    10861 atgccgattt taccaatgcc ctgcccgatt tacgcgcggc aatggcctgg ctaccgccac
    10921 gagaacgcgg gacgctggcg catcaggtgc ttgagcatta tcaactggcg caacttcccg
    10981 tttcggcact gcaaatgccg ttgcattgtc caccgcaagc cattgcacat catcaacaac
    11041 tcgaacagca ggcactggca tcgctgcaac actggggagt tttccatgtc tgaactgaac
    11101 gatcttctga ccacccgtga gctacaacgc tggcgattaa ttcttggcga agcggcagaa
    11161 acgacgcttt gtgggctgga tgacaacgcc cggcagatag atcacgcgct ggagtggctg
    11221 tatgggcgcg atcctgaacg gctccagcgt ggtgaacgct ctagtggatt aggtggctca
    11281 aatctcacca ctcctgagtg gatcaacagt attcacacgc tgtttccgca acaggtgatt
    11341 gagcggctgg aaagcgatgc cgtactgcgc tacggcattg aagatgtggt gacaaatctc
    11401 gacgtgctgg aacgtatgca gccttccgaa agcctgctac gcgccgtttt gcacaccaaa
    11461 catctgatga accccgaagt actggctgcc gcccgcagga ttgtgcgcca ggttgtggaa
    11521 gaaattatgg cgcgactggc aaaggaagtt cgtcaggctt tttctggtgt ccgcgatcgt
    11581 cgtcgccgct catttattcc actggcgcga aactttgatt tcaaaagtac tctgcgcgcc
    11641 aacctgcaac actggcaccc gcaacacggc aagttgtata tcgaattccc ccgctttaac
    11701 agccgcatta aacgccaaag cgagcaatgg caactggtct tactggttga tcaaagcgga
    11761 tcgatggtcg attcggtgat ccactctgcg gtgatggcgg cctgtttatg gcagttaccc
    11821 ggcattcgta cccatctggt ggcgtttgac acaagcgtcg ttgatctcac ggcagacgtt
    11881 gccgatccgg tagagttatt aatgaaagtg caattaggcg gcggaaccaa tatcgccagt
    11941 gccatggagt atggtcggca acttattgaa caacctgcaa aaagcgtcat tatcctcgtg
    12001 agcgattttt atgaaggggg ttcatcatca ttgctgacgc accaagtgaa aaagtgtgtc
    12061 cagagcggca tcaaagtgct gggactggca gcgctcgata gcacagcaac accttgctat
    12121 gaccgcgata cggcccaggc gctggttaat gtcggcgcac aaatagccgc catgacaccg
    12181 ggcgagctgg catcatggct tgcggagaat cttcagtcat gaattcacta cgtccggaat
    12241 tattagaact gacaccgcag gcactgacgg cgttaagcaa tgccggtttt gttaagcgca
    12301 gccttaagga actggaaaat ggcaacgtcc cggaaatcag ccatgagaac agcgctttaa
    12361 tcgccacctt cagtgacggt gtccgtaccc agctggcgaa cggccaggca ctgaaagagg
    12421 ctcagtgcac ctatggggcc agcggcatgt gccgtcatcg cgtgatgctg gtgttaagtt
    12481 atcaacgact ttgtgctacc gcccagccta cgggaaaaga agaggaatgg gatccggcaa
    12541 tctggctgga agaactggct acccttcccg atgccacccg caaacgcgca caggcgctgg
    12601 tcgctaaaag catcaccatt gagttgttct gtgcgccggg tgaaattccc tctgcccgct
    12661 taccgatgag cgatgtgcgt ttttattccc gcagcagtat tcgtttcgcc cgttgtgatt
    12721 gtattgaagg cacactttgc gaacatgtcg tactggcagt acaggccttt gtccaggcaa
    12781 aagcgcagca agcggaattg actcatttaa tctggcagat gcgcagcgaa cacgtcacat
    12841 catctgacga tccgtttgcc agcgaagaag gcaaaacgtg tcgtcaatat gttcaacaac
    12901 taagccaggc attatggctg agcggcatca gccagccgct tattcactac gaggccgctt
    12961 tcagccgcgc gcagcaagca gcggaacgct gcagctggcg atgggtgagt gaatcgctac
    13021 ggcaactgcg cgccagcgtt gatgccttcc acgcccgcgc cagccactat catgccggag
    13081 aatgcttacg tcagcttgcg gcattaaaca gtcgattaaa ttgcgcacaa gagatggccc
    13141 ggagcgacag tgttggtgaa gttcctcctg tgccgtggcg cacggtcgtt ggctctggca
    13201 ttgccggaga agcaaagctt gatcatctgc ggctggtgtc tttaggtatg cgttgctggc
    13261 aggatattga gcattatggt ttacgcatct ggtttaccga tcccgacacc ggcagtattt
    13321 tgcacctttc gcgcagttgg ccgcgaagtg aacaggaaga ctcaccggca gctacgcgtc
    13381 ggctgtttag ttttcaggct ggcgcactgg cgggtgggca aattgtttca caagcagcaa
    13441 aacgcagtgc cgatggcgag ctgctgttag ctacccgcaa ccgcttaagc agcgttgtac
    13501 cgctgtcgcc tgatgcctgg caaatgttga gcgcgccgtt acgccagccg ggcattgtgg
    13561 ctttgcggga atatttacgc cagcgtcccc ccgcctgcat acggcctctt aatcaggtcg
    13621 ataacttatt tattctgccg gtcgctgagt gtatttcgct cggttgggat agcagccgcc
    13681 agacgctgga tgcgcaggta atcagcggcg aaggtgaaga taatctgctg acgttatcac
    13741 tgccagcctc agccagcgcg ccttatgcca ttgaacgcat ggcggcgctt ttgcaacaaa
    13801 cagacgaccc cgtgtgtctg gtttctggct ttgtcagttt tgttgatggg caattgacac
    13861 tggaaccaca ggtaatgatg acaaagactc gtgcctgggc gctggacgca gaaactgcgc
    13921 ctgtggtggt atcactacct tctgccagcg ttctgcctgt gccgtctact gctcatcagt
    13981 tgctgatgcg ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg
    14041 aacagagcgc tattaatcag gcagagttgc tggcgaatga cctcaccgcg gtgggttttt
    14101 atcggctggc acatgtgttg gcacaatttc gtaatacaga aagcgaggca cgggtagaag
    14161 caatgaataa cggtgttttg ctttgcgaac aattattccc catgcttcag caacaaggat
    14221 gaaatagtgc tttttactaa gagttctact ccagttccgg actgctcacg ccacggtatt
    14281 aggcatatcc tatatagccc ctggtgagag tcac
//
LOCUS       DAJTRW010000092        13953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRW010000092 DAJTRW010000000
VERSION     DAJTRW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="OO326_003121"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="OO326_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5967417.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="OO326_003122"
     CDS             2218..2766
                     /locus_tag="OO326_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5967418.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="OO326_003123"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="OO326_003123"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5967419.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO326_003124"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO326_003124"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5967420.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO326_003125"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO326_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5967421.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO326_003126"
     CDS             complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO326_003126"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5967422.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO326_003127"
     CDS             complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO326_003127"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5967423.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9764..12379
                     /gene="pepN"
                     /locus_tag="OO326_003128"
     CDS             9764..12379
                     /gene="pepN"
                     /locus_tag="OO326_003128"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5967424.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO326_003129"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO326_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5967425.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..>13953)
                     /gene="ssuC"
                     /locus_tag="OO326_003130"
     CDS             complement(13350..>13953)
                     /gene="ssuC"
                     /locus_tag="OO326_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5967426.1"
                     /translation="ISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQMLRNV
                     PHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGLSG
                     IPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQTDV
                     VVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3548 a         3560 c         3524 g         3321 t
ORIGIN      
        1 aacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgctggt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgtgacg tggtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccgatcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcgctttc aacgccgccg ctttcggtga ttaaccaatg gcagctagcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggtctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg tgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggagcagat
     1201 ggtgctattg gcccggcaac gcgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggcac tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccagccta ttcgctgctc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcacttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta ccaccaactc tggcacgcaa agatattctg
     1501 ccgaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg gaggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt ttcgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggatgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tgatcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcaa ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgag ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttccattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attacccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat cgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttgatctggt gtgaacaaac ccgcctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatct acacttggtt atgagcgcct gcacaatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa aggcgcataa aaaagtccac ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaac
     3661 gcttacagca ctgccacaat cgattcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaacagca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcagacc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcaccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaatgc atcgctgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggagggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acgatttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcgttcagac tgttaatcag tgcatcgaag
     4381 tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg cttaacgctg
     4501 gtattttttg ccaggaagtc ggcagccacg cgtagtgcgc cagtgccacc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcaag gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggc
     4981 ataaaaaaaa caggaccaac gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gttttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt tatgacacct gccactgccg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggagctttg tgaacaccgt
     6361 catatttcca taggaacgtg atgatattta cagcaatttt tatctattta tatgatttac
     6421 ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat cgacctggca
     6481 caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg tgctatcagc
     6541 taagaaaaaa ctaatccgca ataagagtac gggttatttt ttgtgcgtaa atgttgcaat
     6601 gtaatattcc gtaacaggat gatcgttatg gcgggggtgc aaatagattg cttgacttaa
     6661 caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc tctttgagga
     6721 agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat cacgcacgtt
     6781 ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac ctgaatgcgg
     6841 aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat tcaggcccat
     6901 ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc caccaatgat
     6961 ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt cttcgttgag
     7021 gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg gtgctttaaa
     7081 gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc agtaaaccgg
     7141 gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt aatccacctg
     7201 cgcgaaatcg gcttcaatga agcgttccag acgtgaaacg gcatctttat ctacgcgttc
     7261 agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga catatttcag
     7321 catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt ccggctccag
     7381 catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga aagtcgggcc
     7441 gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact ggccagatac
     7501 ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt
     7561 acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg caccttcggt
     7621 atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg
     7681 atgcagcgcc tgcgccagcg tatggcgaac gcgcgcgacg gcaccaatca ggtttgtgcg
     7741 cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg ccgccatcgg
     7801 gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct gaatttcaaa
     7861 ttgttgcccc tggcccggcg atgccacgac tttacccgtc acaatgaccg agcagccggt
     7921 ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga cagcctgtac
     7981 aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt ttgaatctcg
     8041 gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc ggccctggag
     8101 tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg gaaaaaataa
     8161 acacttgtcc acccgtaaat gggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaag ggagccgatt
     8281 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
     8341 cgcccgaaca aacgctttat catggcagat agttttgcca gggctgtcag aaagtttcgc
     8401 caccggttta ccgttacact ctaccaactt aatcacaata ttcaggggtt ttacctgggg
     8461 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
     8521 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gcgttttact
     8581 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
     8641 gtcgccagag tcatgacgca ggccctgata ccgactagcg aactcgacac cgaaatcacg
     8701 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatactc
     8761 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
     8821 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
     8881 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
     8941 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccatcagat ggaagcgcga
     9001 catatcaaga ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
     9061 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
     9121 cagcaaagga acttcccaga ggatgacttc acgccacgga ccgcttaagc gaatatccag
     9181 cttgccatta tcgttggaca cggtgacttg ttccgggtta aagcggaact cgcgtaacca
     9241 gttaagatag tcggccttaa agaaaggcag ggcagagagc cactgatatt catcatcctg
     9301 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
     9361 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
     9421 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
     9481 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
     9541 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
     9601 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
     9661 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
     9721 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
     9781 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
     9841 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
     9901 tgcatcagat gctccgcttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
     9961 taatgatgag ccgtggaccg cctggaaaga agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcctggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggtaa agtgatcccg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatggtt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tagacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatccgg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccagact
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctgccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggt gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atc
//
LOCUS       DAJTRW010000093        13707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRW010000093 DAJTRW010000000
VERSION     DAJTRW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO326_003131"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO326_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO326_003132"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO326_003132"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5967427.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="OO326_003133"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="OO326_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO326_003134"
     CDS             complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO326_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5967428.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO326_003135"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO326_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5967429.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="OO326_003136"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="OO326_003136"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5967430.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="OO326_003137"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="OO326_003137"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5967431.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8207..9031
                     /gene="rhaD"
                     /locus_tag="OO326_003138"
                     /pseudo
     CDS             8207..9031
                     /gene="rhaD"
                     /locus_tag="OO326_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="OO326_003139"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="OO326_003139"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5967432.1"
                     /translation="MIRKTFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="OO326_003140"
     CDS             9656..10102
                     /locus_tag="OO326_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5967433.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="OO326_003141"
     CDS             10113..11564
                     /locus_tag="OO326_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5967434.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="OO326_003142"
     CDS             11554..12624
                     /locus_tag="OO326_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5967435.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..13274
                     /locus_tag="OO326_003143"
     CDS             12624..13274
                     /locus_tag="OO326_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5967436.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQYPVMLPTY"
     gene            13308..13707
                     /locus_tag="OO326_003144"
                     /pseudo
     CDS             13308..13707
                     /locus_tag="OO326_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         3265 a         3577 c         3609 g         3256 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aaacctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat ccggtaatgc
    13261 tgccaactta ctgatttagt gtatgatggt gattttaagg tgcttgcgtg gcttccattt
    13321 ccatcagatg tccttcctgc tccgctactg aaggcgtggt gcgtaacggc aaaagcactg
    13381 ccggacatca gcgctatctc tgctctcatt gccgtaaaac atggcaacta cagttcactt
    13441 acaccgcctc tcagcccggt acgcaccaga aaatcattga tatggccatg aatggcgtcg
    13501 gatgtcgcgc cagtgcacgc attatgggcg ttggcctcaa cacggtttta cgtcacttaa
    13561 aaaactcagg ccgcagtcgg taacctcgcg catacaaccg ggcagtgatg tgattgtctg
    13621 cgctgaaatg gacgaacatt ggggctacgt cggtgctaaa tcacgtcagc gctggctgtt
    13681 ttacgcgtat gacaggatac ggaggac
//
LOCUS       DAJTRW010000094        13618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRW010000094 DAJTRW010000000
VERSION     DAJTRW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO326_003145"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO326_003145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5967437.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO326_003146"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO326_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5967438.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO326_003147"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO326_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5967439.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO326_003148"
     CDS             3807..3995
                     /locus_tag="OO326_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5967440.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO326_003149"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO326_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5967441.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO326_003150"
     CDS             4756..5115
                     /locus_tag="OO326_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5967442.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO326_003151"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO326_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO326_003152"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO326_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5967443.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO326_003153"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO326_003153"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5967444.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO326_003154"
     CDS             10913..11254
                     /locus_tag="OO326_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5967445.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO326_003155"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO326_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO326_003156"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO326_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5967446.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3195 a         3474 c         3716 g         3233 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaag
//
LOCUS       DAJTRW010000095        13098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRW010000095 DAJTRW010000000
VERSION     DAJTRW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..890)
                     /locus_tag="OO326_003157"
     CDS             complement(<1..890)
                     /locus_tag="OO326_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5967447.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO326_003158"
     CDS             1131..1931
                     /locus_tag="OO326_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5967448.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO326_003159"
     CDS             2009..2779
                     /locus_tag="OO326_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5967449.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO326_003160"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO326_003160"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5967450.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO326_003161"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO326_003161"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5967451.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO326_003162"
     CDS             5034..5756
                     /locus_tag="OO326_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5967452.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO326_003163"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO326_003163"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5967453.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO326_003164"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO326_003164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5967454.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO326_003165"
     tRNA            7149..7225
                     /locus_tag="OO326_003165"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO326_003166"
     CDS             complement(7356..8402)
                     /locus_tag="OO326_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5967455.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO326_003167"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO326_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO326_003168"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO326_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5967456.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO326_003169"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO326_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5967457.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO326_003170"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO326_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO326_003171"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO326_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAJTRW010000096        12996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRW010000096 DAJTRW010000000
VERSION     DAJTRW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..615
                     /gene="mxiE"
                     /locus_tag="OO326_003172"
     CDS             <1..615
                     /gene="mxiE"
                     /locus_tag="OO326_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5967458.1"
                     /translation="VRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAE
                     KCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRT
                     IEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTI
                     TSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            602..2302
                     /gene="mxiD"
                     /locus_tag="OO326_003173"
     CDS             602..2302
                     /gene="mxiD"
                     /locus_tag="OO326_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5967459.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2321..3388
                     /gene="mxiC"
                     /locus_tag="OO326_003174"
     CDS             2321..3388
                     /gene="mxiC"
                     /locus_tag="OO326_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5967460.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3401..5461
                     /gene="mxiA"
                     /locus_tag="OO326_003175"
                     /gene_synonym="virH"
     CDS             3401..5461
                     /gene="mxiA"
                     /locus_tag="OO326_003175"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5967461.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5474..5875
                     /gene="spa15"
                     /locus_tag="OO326_003176"
                     /gene_synonym="spaK"
     CDS             5474..5875
                     /gene="spa15"
                     /locus_tag="OO326_003176"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5967462.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            5879..7171
                     /gene="spaL"
                     /locus_tag="OO326_003177"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5879..7171
                     /gene="spaL"
                     /locus_tag="OO326_003177"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5967463.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            7264..7602
                     /gene="spa13"
                     /locus_tag="OO326_003178"
                     /gene_synonym="spaM"
     CDS             7264..7602
                     /gene="spa13"
                     /locus_tag="OO326_003178"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5967464.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            7589..8467
                     /gene="spa32"
                     /locus_tag="OO326_003179"
     CDS             7589..8467
                     /gene="spa32"
                     /locus_tag="OO326_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5967465.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDTEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            8461..9342
                     /gene="spa33"
                     /locus_tag="OO326_003180"
                     /gene_synonym="spaO"
     CDS             8461..9342
                     /gene="spa33"
                     /locus_tag="OO326_003180"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5967466.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            9347..9997
                     /gene="spa24"
                     /locus_tag="OO326_003181"
                     /gene_synonym="spaP"
     CDS             9347..9997
                     /gene="spa24"
                     /locus_tag="OO326_003181"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5967467.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            10012..10272
                     /gene="spaQ"
                     /locus_tag="OO326_003182"
     CDS             10012..10272
                     /gene="spaQ"
                     /locus_tag="OO326_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5967468.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            10272..11042
                     /gene="spa29"
                     /locus_tag="OO326_003183"
                     /gene_synonym="spaR"
     CDS             10272..11042
                     /gene="spa29"
                     /locus_tag="OO326_003183"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5967469.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            11049..12077
                     /gene="spa40"
                     /locus_tag="OO326_003184"
                     /gene_synonym="spaS"
     CDS             11049..12077
                     /gene="spa40"
                     /locus_tag="OO326_003184"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5967470.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            12090..12332
                     /locus_tag="OO326_003185"
     CDS             12090..12332
                     /locus_tag="OO326_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967471.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         4171 a         1851 c         2532 g         4442 t
ORIGIN      
        1 gtccgaaatc aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt
       61 aatagttgtg caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat
      121 agccatgctg aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat
      181 accgaatcga tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta
      241 agtaatgcac tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg
      301 agaactattg agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg
      361 tcggaggctt attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa
      421 ctgaacactt ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc
      481 acttcagctg ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa
      541 acacgactag gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata
      601 aatgaaaaaa tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt
      661 taatgctaat aatattgact ctcatttatt agaacaaaat gacatagcga aatatgttgc
      721 tcaaagtgat acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat
      781 tgttgttagc aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc
      841 agaagaaatg cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa
      901 tgcattatac atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat
      961 atcacttaat tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc
     1021 tataagagga aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga
     1081 actggtagct aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa
     1141 agttaatttt ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat
     1201 gagaggggag gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa
     1261 tggtaaggct ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac
     1321 acagaaagta tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat
     1381 attagaggat gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa
     1441 tgatcagcaa atacagatta ttcgcgatat tatcactcag ttagatatag ccaaacgaca
     1501 tatagaatta tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt
     1561 taattggcaa ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc
     1621 atcagcatcg ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa
     1681 tcagaaaaag aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc
     1741 ggctatattt gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc
     1801 gttggagcat gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg
     1861 acaaattgag atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa
     1921 ttataataat gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat
     1981 agcgagagtt ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc
     2041 taacgaaata atatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa
     2101 gtataaaaca agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat
     2161 aaaggagtca agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat
     2221 acaaaagact acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt
     2281 ggtttcatac ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac
     2341 aggagttttt agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg
     2401 agatgagact gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc
     2461 atctgatgag atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact
     2521 gaaaggaaca aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa
     2581 tcacaagatt tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt
     2641 tatagacaga ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga
     2701 acttttgaat gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa
     2761 tgagataata tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc
     2821 taaattattt ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg
     2881 ttttatcatg gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg
     2941 ttttaatcac agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga
     3001 ttctgagaaa ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc
     3061 aattaagatg attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt
     3121 gctaaaagat ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca
     3181 atatccaagt gaaagtgagc aaattcttac ttctgttata gaagtatcac gagccagtca
     3241 tgaggattct gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat
     3301 atttaaaagt gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag
     3361 tgcatacaag aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa
     3421 gcaagtaagt actaagcctg aattaattat attagtgctt atggttatga tcattgctat
     3481 gttgatcata ccattaccta cataccttgt tgatttttta atagggctta atattgtact
     3541 tgctattctc gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt
     3601 cccttcggtt ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg
     3661 actaatttta gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat
     3721 tggtgatagt ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat
     3781 tgttataaca aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg
     3841 tatgccgggg aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc
     3901 aggagctaaa gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga
     3961 tggagcaatg aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa
     4021 tttaatagga ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt
     4081 atctacttat accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt
     4141 aatttctata agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg
     4201 acgtaacata atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct
     4261 tgccttggcg atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt
     4321 tactttgacg gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga
     4381 gtctgattct agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt
     4441 ggcaatgata gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt
     4501 tgccgaaaat aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca
     4561 gttctttatt gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct
     4621 taaggtcgat gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata
     4681 ttttgacaaa gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt
     4741 tgtttcaact tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa
     4801 tctaactaat attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc
     4861 acaagcttta ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt
     4921 agatcagttt gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat
     4981 acagagaatt tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt
     5041 aaaacttatt atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt
     5101 agttgaacat gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg
     5161 tgagattaaa gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag
     5221 gcaaacctct ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga
     5281 aactttagca catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt
     5341 atcagtagat atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact
     5401 cgttatatcg tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta
     5461 gtgaggttta aatatgagta acattaattt agttcaatta gttagagata gtcttttcac
     5521 gattggttgt cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc
     5581 tttggatagt atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc
     5641 taattttgat gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt
     5701 aatgttaatg aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga
     5761 gtacttacag ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc
     5821 agagattctg catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat
     5881 gagctataca aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt
     5941 ggaaacaagt cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga
     6001 aagtaatgaa attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt
     6061 aagcttgatt ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca
     6121 gtttcttcat acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga
     6181 ggttactgat aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa
     6241 tgctcctccg ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa
     6301 ggttattgat tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc
     6361 tggttgtggc aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata
     6421 tgttattggg ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa
     6481 aaactctgag aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt
     6541 tgatcgttgt aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg
     6601 acataaagta gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt
     6661 ggccttagcc gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag
     6721 cttacccaga cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt
     6781 ttacactgtt cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc
     6841 cattttagat ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc
     6901 aattgattcc ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg
     6961 tattatggcc gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat
     7021 tgactttggt gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat
     7081 atctgttgtt gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac
     7141 aatggagctt attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac
     7201 ggtctgttaa attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga
     7261 gatgtggagg cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa
     7321 cctgtaggcg ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta
     7381 aaaaagcata ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa
     7441 attcaaaaag atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt
     7501 aaaatatgta aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt
     7561 gatgagttat cacaagagga tatgaagtat ggcattagat aatataaacc taaattttag
     7621 tagtgacaaa cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct
     7681 cgttttgaag aagaagagga aggttgaaat tcctgagtac tctttaattg catctaacta
     7741 ttttactatt gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat
     7801 taaaaatgcg ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat
     7861 ggccattaaa gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa
     7921 tatcggcgat atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt
     7981 cgaaagaagt catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa
     8041 tggtcaaatt ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat
     8101 ttctttacta aatggaagtg ttgctaaatc atttgagctg cctgtaatga ataaaaatac
     8161 tgcagacata accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa
     8221 tgtttttcaa aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc
     8281 tgttagtatt tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg
     8341 aaataaactt gacttaattt tgaaacaaga tactgagggt aattacagat ttgatagcag
     8401 tcaacataat aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga
     8461 atgctaagaa ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa
     8521 ctctgcgagc gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta
     8581 gtcactctta cttccgtgtg tggggattgg gtaattcgta ttgatacatt atcttttttg
     8641 aaaaaaaaat acgaggtatt ttcaggattt tctacacaag aatctttact gcatttatca
     8701 aaatgtgtct ttatagagtc gtcatctgta ttttcgattc cagaactgtc tgataagatt
     8761 actttccgga tcacgaatga aatccagtat gcaactactg ggagtcattt atgctgtttt
     8821 tcctcttctt taggtattat ttattttgac aagatgccgg tattacgtaa tcaagtttct
     8881 cttgactcat tgcatcatct tttagagttt tgcttaggtt catctaatgt aaggctggct
     8941 actttaaaaa gaattcgcac tggtgatata atcatagttc agaaacttta taatttatta
     9001 ttgtgtaatc aagttattat tggggattat attgtgaatg ataataatga ggcaaaaatt
     9061 aatctgtcag aaagtaatgg tgagtcagaa cacacagaag tttctttggc attattcaat
     9121 tatgatgata tcaatgtaaa agtggacttt attcttttag aaaaaaatat gacaatcaat
     9181 gaactaaaaa tgtatgtaga aaacgaatta ttcaagtttc ccgatgacat agttaaacat
     9241 gtaaatatta aagtaaatgg ttctttggtt gggcatgggg aacttgtttc tattgaggat
     9301 ggttatggta tcgagattag ttcttggatg gtaaaggagt aatctcatgc tgagtgacat
     9361 gtccctcatc gctacacttt ctttttttac gctattacca tttttagttg ctgctggtac
     9421 ctgttatata aagttttcta ttgtttttgt aatggtacga aatgcacttg gtttgcagca
     9481 ggttccatca aatatgactc tcaatggtat tgcattgatt atggctttgt tcgtgatgaa
     9541 acccattatt gaagctggtt atgaaaatta tctaaatggc cctcaaaaat ttgatacaat
     9601 atctgatatt gtccgttttt ctgattctgg cttgatggaa tataagcaat acttgaaaaa
     9661 gcatacagat cttgagttag cacgtttttt ccaaaggtct gaagaagaaa atgctgattt
     9721 aaagagtgca gagaataatg attattcatt gttttccctc ttgcctgcat atgcgctaag
     9781 tgaaatcaaa gatgcgttca aaataggttt ttatttatat ttgccatttg ttgttgttga
     9841 tcttgttatt tcaagcattt tacttgcact tggtatgatg atgatgagtc caatcacaat
     9901 atcagtacca ataaaacttg tattatttgt tgcgcttgat gggtggggta tcctgtctaa
     9961 ggccttgatt gaacaatata tcaatattcc tgcttagatg atggaggtaa tgtgtctgat
    10021 atagtttata tgggtaataa ggctctttat ttaatcctta tcttttcctt atggccagta
    10081 ggtatagcta cggttattgg attatctatt ggtttgttac agacagtgac tcaactccaa
    10141 gagcagacac ttccttttgg tataaagctt ataggtgtct caatatcttt gctacttctt
    10201 tctggatggt atggtgaggt tttattgtct ttttgtcatg aaataatgtt tttaattaag
    10261 agtggggttt gatggacatt tcaagctggt tcgaaagtat tcatgtgttt ttaatactcc
    10321 tgaacggcgt tttttttaga ttggctccat tatttttctt tcttccattt ttaaataacg
    10381 gtataatttc tccatctatt agaatacctg tgatttttct tgttgcatca ggattaatta
    10441 cttctggtaa ggtagacata ggttcttctg tttttgaaca tgtttatttc cttatgttca
    10501 aggaaataat tgttggcctc cttctctctt tttgcttgtc tcttcccttt tggatatttc
    10561 atgctgttgg tagcattatt gacaaccagc gtggggcaac gcttagtagt tcaattgatc
    10621 ctgccaatgg tgttgatacg tctgagttgg caaaattttt caatcttttt tctgcagttg
    10681 tatttctata cagtggtggt atggtcttta ttttagaatc catacaattg tcttataata
    10741 tatgcccgtt attttctcaa tgttctttcc gtgtctcaaa tatcttaaca tttctgactt
    10801 tattggcaag tcaggctgtt attttagcca gtcctgttat gatagtattg ttactatcag
    10861 aagtattact tggtgtatta tcgagatttg ctccgcagat gaatgctttt tccgtatcat
    10921 taactattaa aagtttactt gcaatattta ttattttcat ctgttcttct actatttact
    10981 tttctaaagt tcaatttttc ctcggtgaac ataaattttt cacaaatcta tttgttagat
    11041 aaaatattat ggcaaataaa acagaaaagc cgacacctaa aaaactaaag gatgccgcaa
    11101 aaaaaggaca gtcatttaaa tttaaggatt taacgactgt tgttattatt ctggtaggga
    11161 catttactat aatatcattc ttttccttaa gtgatgtaat gctcttatac agatatgtaa
    11221 taattaatga cttcgaaatt aatgagggta aatacttttt tgcagtggtt attgtctttt
    11281 ttaagataat tggcttccca ctttttttct gtgttctttc ggctgtgttg ccaacattgg
    11341 ttcaaacaaa gtttgttctt gcgactaaag ctatcaagat tgatttttca gtattaaacc
    11401 ctgttaaagg gttaaaaaaa atatttagta taaagacaat aaaagaattt ttcaaaagca
    11461 ttctgcttct tattattcta gcattaacaa cctatttctt ttggattaat gaccgaaaaa
    11521 taattttttc tcaggtgttt tctagtgttg atggcttata tcttatttgg gggaggctgt
    11581 ttaaggatat aatattattt ttcttggcat tttctattct tgttattatt cttgactttg
    11641 tgattgagtt cattttatac atgaaagata tgatgatgga taaacaggag ataaaaagag
    11701 aatatataga gcaagaggga cactttgaga caaagtcgag aaggcgtgag ttgcatatcg
    11761 agattctttc agagcagact aaatctgata tacgtaattc aaaattagtg gtaatgaacc
    11821 cgactcatat tgcaattggt atttatttta atccagaaat agcgcctgca ccttttattt
    11881 ctctcattga aactaaccag tgtgccttgg ctgtcagaaa atatgcaaat gaagttggta
    11941 taccgactgt gcgtgatgtg aaattagcta gaaaactata taaaacacat acaaaatata
    12001 gttttgttga ttttgaacac ttggatgaag tcctacgtct tattgtttgg cttgagcagg
    12061 ttgaaaacac tcattaataa gtaaaggaga tgtgttatat gggagttaat ttctgtaata
    12121 aaataggtat tgatcagtct gaatttgaaa tagaatcttc catcattaac tccattgcta
    12181 atgaggtatt gaacccaata tctttcctta gcaataagga tataataaat gttttactca
    12241 gaaagatttc atctgaatgt gacctggtta gaaaagacat ttatcgttgt gctctggagt
    12301 tggtcgttga aaaaacacct gatgatttat aatcattcat aataactctt ttgctgatat
    12361 aaatattttt gttgaagata tttatgtagt cacaggatca attgtagaaa attgttatgt
    12421 ttttgttagg aactgcacta aataatatat gataagacag caaaaacgac tgaccataat
    12481 ccttctttta ttaggagttg ataagagaga ctattcatcc tgtaatgtta aaactctgtt
    12541 atatagtatt cgcgattatg caaagtctgt aaatgatcat gagatactaa ccgagagtaa
    12601 tcgtttatta tctcattgca tttcagatag taacggagct ttctttaaaa gtagtaaata
    12661 cgttcccctt aagtacttac gtaaaaggag aatagcacga aagataccaa atgattagca
    12721 ctttgctagt aagcttgctg gtaaactata ttgaccgttg ggtttgtttt gagactgtaa
    12781 tttaaattgt gtaattgcct gtttttgata tgtttactcc aacaacggag acaggtaaat
    12841 tatggacgaa aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaactga
    12901 ggccgacctc aatcagtttt cccgtatgct gacgaaatat cccggctctt aaaacgatag
    12961 tggctcaggc tttcattttt cagcgttccc cagaag
//
LOCUS       DAJTRW010000097        12898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRW010000097 DAJTRW010000000
VERSION     DAJTRW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO326_003186"
     CDS             complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO326_003186"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5967472.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1796..3214)
                     /locus_tag="OO326_003187"
     CDS             complement(1796..3214)
                     /locus_tag="OO326_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5967473.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3368..3712)
                     /locus_tag="OO326_003188"
     CDS             complement(3368..3712)
                     /locus_tag="OO326_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5967474.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO326_003189"
     CDS             complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO326_003189"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5967475.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4702..5637)
                     /locus_tag="OO326_003190"
     CDS             complement(4702..5637)
                     /locus_tag="OO326_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5967476.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5717..6832
                     /gene="yneK"
                     /locus_tag="OO326_003191"
     CDS             5717..6832
                     /gene="yneK"
                     /locus_tag="OO326_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5967477.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6982..8172
                     /gene="ydeA"
                     /locus_tag="OO326_003192"
     CDS             6982..8172
                     /gene="ydeA"
                     /locus_tag="OO326_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5967478.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO326_003193"
     CDS             complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO326_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5967479.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9074..9508
                     /gene="marR"
                     /locus_tag="OO326_003194"
     CDS             9074..9508
                     /gene="marR"
                     /locus_tag="OO326_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5967480.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9528..9911
                     /gene="marA"
                     /locus_tag="OO326_003195"
     CDS             9528..9911
                     /gene="marA"
                     /locus_tag="OO326_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5967481.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9943..10161
                     /gene="marB"
                     /locus_tag="OO326_003196"
     CDS             9943..10161
                     /gene="marB"
                     /locus_tag="OO326_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5967482.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO326_003197"
     CDS             complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO326_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5967483.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11355..12473
                     /gene="ydeE"
                     /locus_tag="OO326_003198"
     CDS             11355..12473
                     /gene="ydeE"
                     /locus_tag="OO326_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5967484.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12600..12695
                     /gene="mgtS"
                     /locus_tag="OO326_003199"
     CDS             12600..12695
                     /gene="mgtS"
                     /locus_tag="OO326_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5967485.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3398 a         3297 c         2948 g         3255 t
ORIGIN      
        1 caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata ggatgttgta
       61 actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
      121 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
      181 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      241 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      301 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      361 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      421 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      481 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      541 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      601 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      661 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      721 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      781 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      841 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      901 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      961 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
     1021 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
     1081 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1141 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1201 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1261 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1321 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1381 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1441 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1501 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1561 tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt ggtgcgacag
     1621 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1681 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1741 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1801 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1861 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
     1921 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
     1981 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
     2041 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
     2101 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
     2161 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
     2221 gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt
     2281 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga
     2341 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
     2401 gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat
     2461 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
     2521 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
     2581 cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc atcatctgtt
     2641 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
     2701 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
     2761 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
     2821 agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc agacgcttat
     2881 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
     2941 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
     3001 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
     3061 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
     3121 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
     3181 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
     3241 tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt cgctaacagc
     3301 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
     3361 gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
     3421 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
     3481 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
     3541 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
     3601 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
     3661 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
     3721 cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc
     3781 tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac
     3841 aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc
     3901 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
     3961 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
     4021 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
     4081 tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
     4141 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
     4201 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa
     4261 catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa taccctgttc
     4321 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
     4381 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
     4441 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
     4501 acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
     4561 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
     4621 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
     4681 atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa
     4741 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
     4801 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
     4861 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
     4921 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc
     4981 aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
     5041 cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta acaaacgcgc
     5101 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
     5161 cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
     5221 tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc
     5281 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
     5341 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
     5401 ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     5461 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     5521 cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca tcacattcgg
     5581 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     5641 ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg atgacttatg
     5701 actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat ctcttcctga
     5761 tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg attttattga
     5821 tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca ccaactcagc
     5881 cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga cttcttttcg
     5941 gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc ctgtcaagaa
     6001 tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc ctatttattt
     6061 agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc caaagcctgt
     6121 cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt ttatgaatga
     6181 ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag cgcatacacc
     6241 acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac gccagtttga
     6301 tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc gattatggaa
     6361 taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca ccgccattct
     6421 cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg attcattagg
     6481 ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt tagcagaaca
     6541 agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat tgcactatca
     6601 ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag catcattaag
     6661 tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg taaaaaatga
     6721 aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct cccctgtcag
     6781 accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat
     6841 gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa cgcgatccag
     6901 atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg
     6961 caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta
     7021 cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt
     7081 ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg
     7141 ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt aatgaccagt
     7201 caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta
     7261 ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt
     7321 gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc tccggccggg
     7381 aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat ggtcttaggt
     7441 ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt cttcgcgatt
     7501 ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt actgcctagt
     7561 gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc
     7621 atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag
     7681 ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta
     7741 ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg
     7801 tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct
     7861 gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg
     7921 atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc taccgacgtc
     7981 gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc gttggtaggt
     8041 aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct
     8101 tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa
     8161 cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt taatgatttc
     8221 caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg
     8281 ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg agctgcgtag
     8341 gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac
     8401 ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat
     8461 ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat tggcactggg
     8521 ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg
     8581 cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc
     8641 gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg ccaccatcat
     8701 gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat
     8761 gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta acggcagcaa
     8821 caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt
     8881 gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag
     8941 ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca
     9001 attcattcat ttgacttata cttgcctggg caatattacc ccctacaact aattacttgc
     9061 caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg
     9121 cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct
     9181 ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac
     9241 tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga
     9301 tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata agcgcggcgt
     9361 actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc aattagttgg
     9421 ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg caacacttga
     9481 gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg tccagacgca
     9541 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
     9601 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
     9661 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
     9721 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
     9781 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc
     9841 ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca ttaaatcatt
     9901 acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc actttcatcc
     9961 gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca aaccacgcag
    10021 ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga acaaccaccg
    10081 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    10141 tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt attaacttcc
    10201 cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10261 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10321 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10381 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10441 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10501 atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10561 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10621 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10681 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10741 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10801 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    10861 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    10921 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    10981 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    11041 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    11101 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11161 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11221 tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct ggtagagcgt
    11281 cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt attttagaga
    11341 tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11401 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    11461 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11521 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    11581 ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11641 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11701 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11761 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    11821 aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    11881 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    11941 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    12001 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    12061 cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg ctgatgacag
    12121 caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc aacaacctgc
    12181 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12241 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12301 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12361 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12421 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    12481 cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa tatctttctc
    12541 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    12601 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    12661 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    12721 ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaccg
    12781 ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt tacgctaaca
    12841 ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt ctcaatga
//
LOCUS       DAJTRW010000098        12772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRW010000098 DAJTRW010000000
VERSION     DAJTRW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(280..999)
                     /locus_tag="OO326_003200"
                     /pseudo
     CDS             complement(280..999)
                     /locus_tag="OO326_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1015..1347)
                     /gene="cusF"
                     /locus_tag="OO326_003201"
     CDS             complement(1015..1347)
                     /gene="cusF"
                     /locus_tag="OO326_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5967486.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1371..2753)
                     /gene="cusC"
                     /locus_tag="OO326_003202"
     CDS             complement(1371..2753)
                     /gene="cusC"
                     /locus_tag="OO326_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5967487.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2910..3593
                     /gene="cusR"
                     /locus_tag="OO326_003203"
     CDS             2910..3593
                     /gene="cusR"
                     /locus_tag="OO326_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5967488.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3583..5025
                     /gene="cusS"
                     /locus_tag="OO326_003204"
     CDS             3583..5025
                     /gene="cusS"
                     /locus_tag="OO326_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5967489.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5175..7412
                     /gene="nrfB"
                     /locus_tag="OO326_003205"
     CDS             5175..7412
                     /gene="nrfB"
                     /locus_tag="OO326_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5967490.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7399..10367
                     /gene="nfrA"
                     /locus_tag="OO326_003206"
                     /pseudo
     CDS             7399..10367
                     /gene="nfrA"
                     /locus_tag="OO326_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10368..11258
                     /locus_tag="OO326_003207"
     CDS             10368..11258
                     /locus_tag="OO326_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5967491.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11441..12202
                     /gene="envY"
                     /locus_tag="OO326_003208"
     CDS             11441..12202
                     /gene="envY"
                     /locus_tag="OO326_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5967492.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12248..12324)
                     /locus_tag="OO326_003209"
     tRNA            complement(12248..12324)
                     /locus_tag="OO326_003209"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12288..12290),aa:Arg,seq:tct)
     gene            12567..>12772
                     /locus_tag="OO326_003210"
     CDS             12567..>12772
                     /locus_tag="OO326_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5967493.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3127 a         3313 c         3240 g         3092 t
ORIGIN      
        1 gcatcagcca attcttgtgg gccagaatat ggtttaccat aaatagtacg cagttttgtg
       61 tcgcttatag ccataaataa tatttggtac gctcagaaat aatggtttgg tacacatctg
      121 gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac
      181 atactcaggt gaaaaaaagg ttgtttttcg agttataaca gacagttaat taactacagt
      241 ggacaatgct agacaatgac agacacagat aagcaaccta ccttcctctt tcgcgaccac
      301 gttatctttg gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat
      361 gggcgctttg agcgtaaagc gagtctggat tttttgcgtg gcgatcaggt ggcgaatatc
      421 cgcctccggc attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt
      481 accgccggtt tcgcgcaaca gtaaatactc actctgcgct tccacccagt cgggaatggt
      541 caggtcgaga agcggtgtgc ccttttgtac tttatcgccc acggtaagcg gatacacctt
      601 gtcgataaac ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac
      661 attcgtcggg aaactctggg caaaagtcag cggcccgcgc gtgacggtag ccgttttcac
      721 ccccagattc tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc
      781 ggcatatttc ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg
      841 cgtattggga tacatcgggt cgtaccagaa taagatttta cgttctgcgg tcggcatttt
      901 ttctgcgtgc ggttccgcct ggtcaaacca ggtaaaacct gccgctgaaa taataccgcc
      961 cgcgatcatg ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg
     1021 gctgacttta atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac
     1081 tttgtcgccg gttttaattt cactcatttt cgtctgcggg gtgatggtaa agcgcatggt
     1141 catctccggc cagttgacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc
     1201 cagatcaaca ccttttacca caccggtggc actaatgacc tgtggttgtg cttcgctcat
     1261 ggtttcatga tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa
     1321 cattgcgact tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc
     1381 caaccgcccc ctaaggcggt atacaaataa atttcgttaa cctgacgggc ataattcagg
     1441 tcaagtaaag tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa
     1501 cttaccgcgc cgtgctgata taatgcccgc gcccgttgca gggtaatttg cagcgacgcc
     1561 agataacgct gctgggcgct gatttgatcg tccaggcttt gacgtaatgc cagcgcatct
     1621 gccacctctt taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg
     1681 cgaatttcgg cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca
     1741 attttaggaa taaaattcca catcccgctg ctagcgttaa ataatgacga cagatcgctg
     1801 ctggcggttg atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca
     1861 ccaatattag cattagccgc cattaacgcg tgttcagctt ccataatatc cgggcgctgc
     1921 aataagattt gcgacgacaa gcccgccggt aatttaacgc tttgcaggct gccgctgttt
     1981 actgtctgcg cttgcggtag cttgccgtag cttcccaata acagttgcaa tgcattattc
     2041 gcctgcgcca gttccccctg acgtttagcg atgtcactgc gggtactttc tatcaccccg
     2101 cgagcctgtt ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac
     2161 gcatatgact gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc
     2221 agctgctgat tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc
     2281 tgggcttcct cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcga
     2341 ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct
     2401 gaatcgcctt taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag
     2461 cggtcggcat cggtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg
     2521 cgcaaatccc ggttattcac cagcgcctcg ctaatcagcg tcttcacctg attatcaaca
     2581 aaaaaggtgc gccagcccac gttctggtag ttatccgctg cgttaaccag gccgttctgg
     2641 ctgagtgaga actgctgcgg tacgggcatt accggacgct gataatccgg tgccagtgaa
     2701 caaccagtta gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca
     2761 taatttctgg tgattttatg tcgccaactt tactcgccag gctctgattt tccggtgaca
     2821 ggaaaatgac aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta
     2881 gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga
     2941 aaaccggaga atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg
     3001 acaacgggct gaatggctac catctggcga tgaccggtga ttatgacctg ataatcctcg
     3061 atattatgct gccagacgtg aacggctggg atatcgtgcg catgttgcgc tccgccaata
     3121 aagggatgcc gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc
     3181 tggagttggg ggcagatgac tacctggtga aaccgttcgc ttttgctgaa ctgctggcgc
     3241 gggtgcgcac tctcctgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg
     3301 ccgatctgat ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc
     3361 tgaccagtaa agaatttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc
     3421 cccgctcgct tatcgcctcg caggtatggg acatgaattt cgacagcgac actaacgcca
     3481 ttgatgtggc ggtgaagctg ctacgcggca aaatcgacaa cgactttgag ccgaagctga
     3541 ttcaaaccgt gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt
     3601 cagcgcccgt tttcgctggc aacccgcctg acctttttta tcagccaggc caccatcgcg
     3661 gcgtttttcg cctttgcatg gatcataatc cactcagtaa aagtgcattt tgccgagcag
     3721 gacattaatg atttaaaaga gattagcgcc acccttgaac gggtactaaa tcaccctgac
     3781 gaaacgcaag cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg
     3841 ttgatttccc tggcagatag ccacggcaaa acggtgtatc actcccccgg tgcgcctgat
     3901 atccgcgagt ttgcacgtga tgccataccc gataaagacg cgcggggtgg cgaggtgtat
     3961 ctcctttccg gcccgacgat tatgatgcca ggccacggtc acgggcatat ggaacacagc
     4021 aactggcgga tgattaactt gccggttggc ccgttggtgg acggcaaacc gatttatacg
     4081 ctctacatcg cgctgtcgat cgattttcat cttcattaca taaatgattt gatgaataaa
     4141 cttattatga ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta
     4201 cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa
     4261 gatctcgacg ttcgccttga cccgcagacc gtgccgattg agctggaaca gctggtactg
     4321 tcgttcaacc atatgatcga gcgtattgag gatgtcttta ctcgccagtc caatttctca
     4381 gcggatatcg cccacgaaat tcgcacacca attactaatc tcattacgca aacggaaatc
     4441 gccctcagcc agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa
     4501 gagctgacgc gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac
     4561 aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc
     4621 gattttttcg aggcgctagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa
     4681 cgtcaggttg cgggcgatcc gttgatgctg cgtcgggcgc taagcaacct gctttctaat
     4741 gcccttcgtt atacgccaac cagagagaca attgtagtac gctgtcagac ggtcgatcac
     4801 caggtgcaag tttccgtcga aaaccccggt acgcccattg cgcccgagca cttaccgcga
     4861 ttgtttgacc gtttctatcg cgttgacccc tcccgccagc gaaaaggtga aggtagcggt
     4921 attgggctgg cgatagtgaa atcgattgtt gtcgcgcata aaggcacggt tgcggtaacg
     4981 tcagatgcgc gggggacaag gtttgttatc acattacccg cttaattatt cagcaaacgg
     5041 caacttttat aaccagtgta aaaataacgt gccgcaatag tcatacatat taatggtaaa
     5101 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
     5161 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
     5221 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
     5281 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
     5341 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
     5401 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
     5461 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg
     5521 atgttgacga agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg
     5581 gtcccaccag caaagccgac tgcctgaaca acgtgctgga cgccatcacc cagtttgaac
     5641 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
     5701 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag
     5761 tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
     5821 cagaactgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg
     5881 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtaacg
     5941 gtattgcttt cgacgtgcag agtcttactg aagattacga cattggcttc cgcctgaaag
     6001 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
     6061 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     6121 tccccgatac cttttcgact gcggttcgac aaaaatctcg ctggatcatc ggcattgttt
     6181 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     6241 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     6301 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     6361 tttttagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta
     6421 accgcatcgt gcagcgagtg attttcgtca ctggctacta cggcctcacg cagggcctgc
     6481 tttccgtcct gcgtcttttc tggggcaacc tgattaactt tatggctaac tggcgcgtgc
     6541 tgaaacaggt tcttcagcac ggcgatccgc gtcgcgtcgc gtgggataaa acaacgcatg
     6601 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     6661 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
     6721 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg
     6781 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct
     6841 cgctgattgc cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc
     6901 tggaaaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     6961 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     7021 tcacagggtt acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     7081 acaatgcggt tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat
     7141 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     7201 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     7261 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     7321 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     7381 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     7441 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     7501 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     7561 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     7621 caacaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgtcatttt
     7681 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     7741 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt
     7801 gaagaactgc ttgcccagca aaaagcgggc gacgctgcgc cgaccctgcg ttgtcgcagt
     7861 gaagtcgggc agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac
     7921 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     7981 gcaatctacc tgaaacaatg atcccaggca gatacgctat acaatgaagc acgccagcag
     8041 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     8101 gacgatcgga tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt
     8161 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     8221 gaaaataagc cgctgtttac cacgggcgca caagagaaaa gttggctcta tctgttatct
     8281 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     8341 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     8401 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     8461 agcgtggcga cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa
     8521 gaaccgacaa atcttccccg cctggatcaa ctgacctggc aactgatgca gaacgagcag
     8581 tcacgcgaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc
     8641 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctagcaacg
     8701 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
     8761 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     8821 tcgccttcct acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     8881 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgag cgcctggcaa
     8941 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     9001 tggcagaaaa tcagtcttca cgacatgagc aatgatgatc tgctcgccgc cgccaacacc
     9061 gctcaggcag cgggaaatgg cgcagctcgc gatcgctggc ttcaacaggc agaacaacgt
     9121 gagctgggaa acaatgctca atactggtgg ctgcatgcgc gacgttatat cccagctcag
     9181 ccagaactcg cactgaacga tctcacgcgc tcaatcaata ttgccccttc tgccaactct
     9241 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg ttccggccgc ggtgagtgat
     9301 ttgcgcgccg cgctggaact ggaaccaaat aatagcaaca cccaggcagc gcttggttac
     9361 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     9421 gggcttccgg acgatccggc actgatccga caactggcct acgtgaacca gcgtctggat
     9481 gacatgcctg cgacgcagca ctacgcccgg ctcgtgattg atgacattga taatcaggca
     9541 taaccccact gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg
     9601 aggtcggtcg ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa
     9661 tgagtaccgc taacaataat gtcggcggtg cagcgcctgg gaaaagctat cgtagctacg
     9721 gacaactgga agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct
     9781 cagtttatag ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa
     9841 atccgatgtc cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg
     9901 ccgtcgaaca gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg
     9961 ccagcgcctc attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag
    10021 gctggtttgc ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg
    10081 cgtggacggc agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg
    10141 agccttacgc tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc
    10201 ttggcggtgt cggggtccgc tggaatattt ggaccggcga gacgcactac gacgcctggc
    10261 cgcacaaagt cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta
    10321 acggagagcg caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca
    10381 ttttcgtatt gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta
    10441 tctggcaacc acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga
    10501 gtcagttacg ttttcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg
    10561 catttaccca gccagaacag cgcacgttat tgtttaagcg tgccgcagct gcgcaacaga
    10621 ctggcctgaa gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac
    10681 agtcgtctgc agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag
    10741 ccagattatg gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa
    10801 ttgacgacct gaactggcgc agcgaagccg cccgtcaacc tttgctaaca tggttaaaca
    10861 acgcgcagcg gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg
    10921 ccggaaacat gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggag
    10981 ttaatgtctg ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac
    11041 gttatctaca ggccagcgcc gattgccaaa gctccgcccc agcaagcggc attgtttatg
    11101 aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa
    11161 ttgcctcgct gttagcgaaa cgttcctctt gcggtaaaga catgctctat ttctctctgc
    11221 gctatttgcc cgtcgcgcac ggcattctcg agtattaaat cccctccagg taagtcgggt
    11281 acgacctggc ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag
    11341 catgctaaag aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata
    11401 gatcctttga gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga
    11461 accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac
    11521 gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact
    11581 ctcaacgtta aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct
    11641 gttgttttta aataagaaat taacctgtgt aaagccctgg tcgcggctag caaccccggt
    11701 tatcgcttgt catagccgta caccggaagt gttccggcta gccaccaacc acagcaagca
    11761 gcaatccagc aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc
    11821 taactttctt gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt
    11881 ccgcgacagc gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat
    11941 tgtcgccagt tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa
    12001 taccagctat agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt
    12061 gatggataac aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta
    12121 ctttatctct gtttttaagg cgttttacag cctgacaccg ttgaattatc tcgccaaaca
    12181 gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa
    12241 gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc
    12301 tctatccaac tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat
    12361 acggtcaacc cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg
    12421 cgtctgattt tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct
    12481 gaatcaagaa aacccgtatt ttcatgtatc aaagtacaat ttaccgccct aacggaaaat
    12541 tgtccgctcc tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc
    12601 atcctatcat cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg
    12661 tcctgaaaac ggatgattac cgcataacca tcgattatct ccgaacccgt cctgttgatt
    12721 taatcattat ggatatagac ttgcccggaa cagacggttt taccttcctg aa
//
LOCUS       DAJTRW010000099        12696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRW010000099 DAJTRW010000000
VERSION     DAJTRW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO326_003211"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO326_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5967494.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO326_003212"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO326_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5967495.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO326_003213"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO326_003213"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5967496.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO326_003214"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO326_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5967497.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO326_003215"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO326_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5967498.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO326_003216"
     CDS             4025..4615
                     /locus_tag="OO326_003216"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5967499.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO326_003217"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO326_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5967500.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO326_003218"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO326_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5967501.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO326_003219"
     CDS             complement(6551..6658)
                     /locus_tag="OO326_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967502.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO326_003220"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO326_003220"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5967503.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO326_003221"
     CDS             7648..7875
                     /locus_tag="OO326_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5967504.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO326_003222"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO326_003222"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5967505.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO326_003223"
     CDS             complement(9447..10703)
                     /locus_tag="OO326_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5967506.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO326_003224"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO326_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5967507.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO326_003225"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO326_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5967508.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO326_003226"
     CDS             complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO326_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5967509.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3241 c         3200 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgtg
//
LOCUS       DAJTRW010000100        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRW010000100 DAJTRW010000000
VERSION     DAJTRW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            118..1815
                     /gene="maeA"
                     /locus_tag="OO326_003227"
     CDS             118..1815
                     /gene="maeA"
                     /locus_tag="OO326_003227"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5967510.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1949..2959
                     /gene="adhP"
                     /locus_tag="OO326_003228"
     CDS             1949..2959
                     /gene="adhP"
                     /locus_tag="OO326_003228"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5967511.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3105..3389
                     /locus_tag="OO326_003229"
     CDS             3105..3389
                     /locus_tag="OO326_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5967512.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO326_003230"
     CDS             complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO326_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5967513.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO326_003231"
     CDS             complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO326_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5967514.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO326_003232"
     CDS             complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO326_003232"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7620..7622),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5967515.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8439..9320
                     /gene="yddG"
                     /locus_tag="OO326_003233"
     CDS             8439..9320
                     /gene="yddG"
                     /locus_tag="OO326_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5967516.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9575..10675
                     /gene="ompC"
                     /locus_tag="OO326_003234"
     CDS             9575..10675
                     /gene="ompC"
                     /locus_tag="OO326_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5967517.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10839..11324
                     /locus_tag="OO326_003235"
     CDS             10839..11324
                     /locus_tag="OO326_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5967518.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11434..12423
                     /locus_tag="OO326_003236"
     CDS             11434..12423
                     /locus_tag="OO326_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5967519.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3336 a         3126 c         2940 g         3250 t
ORIGIN      
        1 accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac gccctggcgg
       61 taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg
      121 gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg
      181 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
      241 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      301 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      361 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      421 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      481 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      541 gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      601 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      661 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      721 caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      781 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      841 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      901 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      961 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
     1021 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
     1081 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
     1141 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1201 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1261 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1321 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1381 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1441 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1501 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1561 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1621 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1681 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1741 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1801 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1861 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1921 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1981 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     2041 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     2101 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     2161 cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag gatgcggtca
     2221 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2281 cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2341 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2401 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2461 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2521 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2581 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2641 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2701 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2761 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2821 cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt
     2881 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2941 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     3001 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     3061 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     3121 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3181 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3241 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3301 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3361 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg ccatttgaaa
     3421 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3481 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3541 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3601 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3661 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3721 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3781 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3841 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3901 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3961 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     4021 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     4081 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     4141 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4201 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4261 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4321 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4381 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4441 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4501 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
     4561 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4621 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4681 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4741 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4801 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4861 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4921 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4981 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     5041 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     5101 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     5161 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5221 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5281 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5341 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5401 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5461 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5521 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5581 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5641 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5701 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5761 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5821 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5881 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5941 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     6001 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     6061 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     6121 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6181 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6241 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6301 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6361 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6421 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6481 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6541 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6601 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6661 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6721 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6781 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6841 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6901 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6961 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     7021 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     7081 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     7141 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7201 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7261 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7321 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7381 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7441 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7501 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7561 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7621 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7681 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7741 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
     7801 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7861 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7921 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7981 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     8041 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     8101 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     8161 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8221 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8281 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8341 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8401 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8461 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8521 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8581 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8641 ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg tatgcggcga
     8701 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8761 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8821 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8881 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8941 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     9001 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     9061 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     9121 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9181 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9241 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9301 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9361 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9421 gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc
     9481 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9541 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9601 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9661 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9721 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9781 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9841 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9901 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9961 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
    10021 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
    10081 tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt atggtgctga
    10141 cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10201 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10261 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10321 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10381 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10441 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10501 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10561 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10621 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10681 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10741 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10801 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10861 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10921 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10981 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    11041 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    11101 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    11161 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11221 tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11281 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11341 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11401 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11461 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11521 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11581 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11641 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11701 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11761 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11821 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11881 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11941 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    12001 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    12061 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    12121 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12181 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12241 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12301 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12361 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12421 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12481 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat
    12541 aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat acacacttac
    12601 aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTRW010000101        12637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRW010000101 DAJTRW010000000
VERSION     DAJTRW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..1040
                     /locus_tag="OO326_003237"
     CDS             <1..1040
                     /locus_tag="OO326_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5967520.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO326_003238"
     CDS             1041..3206
                     /locus_tag="OO326_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5967521.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO326_003239"
     CDS             3220..4131
                     /locus_tag="OO326_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5967522.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO326_003240"
     CDS             4131..5426
                     /locus_tag="OO326_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5967523.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO326_003241"
     CDS             5471..5701
                     /locus_tag="OO326_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967524.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO326_003242"
     CDS             5679..6179
                     /locus_tag="OO326_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5967525.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO326_003243"
     CDS             6179..7597
                     /locus_tag="OO326_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5967526.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO326_003244"
     CDS             7597..8445
                     /locus_tag="OO326_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967527.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO326_003245"
     CDS             8445..8900
                     /locus_tag="OO326_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5967528.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO326_003246"
     CDS             8903..9595
                     /locus_tag="OO326_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5967529.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO326_003247"
     CDS             9605..10936
                     /locus_tag="OO326_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5967530.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12637
                     /locus_tag="OO326_003248"
     CDS             10937..>12637
                     /locus_tag="OO326_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5967531.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     D"
BASE COUNT         3398 a         3127 c         3332 g         2780 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gatgcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
    12601 ggtggcagaa gatcttggcg ggatgaaaaa ccttgac
//
LOCUS       DAJTRW010000102        12302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRW010000102 DAJTRW010000000
VERSION     DAJTRW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            508..591
                     /locus_tag="OO326_003249"
     CDS             508..591
                     /locus_tag="OO326_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5967532.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO326_003250"
     CDS             complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO326_003250"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5967533.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO326_003251"
     CDS             complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO326_003251"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5967534.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3114..5156
                     /gene="prlC"
                     /locus_tag="OO326_003252"
     CDS             3114..5156
                     /gene="prlC"
                     /locus_tag="OO326_003252"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5967535.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO326_003253"
     CDS             5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO326_003253"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5967536.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO326_003254"
                     /pseudo
     CDS             complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO326_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO326_003255"
     CDS             complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO326_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5967537.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8580..8915
                     /gene="uspB"
                     /locus_tag="OO326_003256"
     CDS             8580..8915
                     /gene="uspB"
                     /locus_tag="OO326_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5967538.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO326_003257"
     CDS             complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO326_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5967539.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10717..11916
                     /locus_tag="OO326_003258"
     CDS             10717..11916
                     /locus_tag="OO326_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5967540.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3018 a         3119 c         3272 g         2893 t
ORIGIN      
        1 gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga
       61 agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat cgggtcgctg
      121 ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc ggttttttta
      181 ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc taacgcatca
      241 atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc taattacact
      301 gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc
      361 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagatt
      421 gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
      481 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
      541 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
      601 cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg catggtcacg
      661 aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcg
      721 cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg
      781 tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg
      841 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
      901 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga
      961 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     1021 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     1081 cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata tttatcgacg
     1141 acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg
     1201 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc
     1261 agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggta
     1321 tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     1381 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     1441 atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc
     1501 aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc tggaatatcc
     1561 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     1621 ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     1681 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     1741 gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata atcagggccg
     1801 tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     1861 ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg
     1921 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     1981 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     2041 acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt
     2101 gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta ttcatctgct
     2161 gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     2221 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     2281 ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag
     2341 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg
     2401 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt
     2461 tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa
     2521 atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt
     2581 gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc
     2641 tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc aaatcgtcct
     2701 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctac
     2761 ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     2821 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     2881 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatt
     2941 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     3001 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     3061 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     3121 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     3181 ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc
     3241 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgttt
     3301 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     3361 gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac
     3421 atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga
     3481 acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc tctggcatag
     3541 gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt tctgaactgg
     3601 gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg
     3661 acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg
     3721 aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac ctgccggtaa
     3781 tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac agcacccgcg
     3841 cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg gaagagatcc
     3901 tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc
     3961 ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa
     4021 aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg
     4081 aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacagaaac
     4141 agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg
     4201 ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     4261 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac
     4321 tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     4381 tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt
     4441 atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg
     4501 aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg
     4561 aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc
     4621 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg
     4681 aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc
     4741 aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg
     4801 ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac
     4861 tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt
     4921 tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag
     4981 atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc
     5041 tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc
     5101 gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat
     5161 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
     5221 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg
     5281 gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt
     5341 gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg
     5401 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
     5461 gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat
     5521 ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
     5581 ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac ggcgctaacc
     5641 gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
     5701 agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
     5761 gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
     5821 ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg
     5881 catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg
     5941 ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag
     6001 ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc
     6061 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc
     6121 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
     6181 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag
     6241 ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga taaaccatgg
     6301 cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc
     6361 cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt ctttgccttt
     6421 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
     6481 gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt
     6541 gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
     6601 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
     6661 gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca gcaccagatt
     6721 ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag
     6781 taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc
     6841 tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca
     6901 caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc
     6961 tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg
     7021 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
     7081 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc
     7141 ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc
     7201 caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa
     7261 agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac
     7321 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
     7381 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat taatatttat
     7441 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
     7501 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
     7561 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa ttttgtattg
     7621 gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca
     7681 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     7741 tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg
     7801 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     7861 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcctaggtcg
     7921 ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag ctcggtcagc
     7981 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8041 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag
     8101 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgact
     8161 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8221 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     8281 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     8341 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atattatatt
     8401 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     8461 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     8521 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     8581 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     8641 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     8701 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     8761 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     8821 gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg
     8881 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa taacgtataa
     8941 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9001 ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa acacccaggc
     9061 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9121 cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat aactcgccag
     9181 gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9241 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9301 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9361 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac caggcatctt
     9421 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     9481 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     9541 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     9601 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     9661 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     9721 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
     9781 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
     9841 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
     9901 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
     9961 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10021 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10081 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10141 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10201 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10261 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10321 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10381 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    10441 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    10501 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    10561 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    10621 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    10681 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
    10741 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt aggacgccgg
    10801 gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc tggcggtggg
    10861 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
    10921 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
    10981 cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    11041 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt cagattgcgt
    11101 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
    11161 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
    11221 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    11281 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    11341 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta
    11401 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag ctactggcaa
    11461 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    11521 gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg
    11581 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
    11641 ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    11701 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    11761 ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggtgaagtg
    11821 atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    11881 gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc cgtcagccgt
    11941 ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt ctggtgttaa
    12001 ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat agtgtattta
    12061 ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa ggaaattatt
    12121 tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc ctctggattg
    12181 aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt atcaggtggt
    12241 gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa tatggctatt
    12301 cc
//
LOCUS       DAJTRW010000103        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRW010000103 DAJTRW010000000
VERSION     DAJTRW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..492)
                     /locus_tag="OO326_003259"
     CDS             complement(<1..492)
                     /locus_tag="OO326_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5967541.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO326_003260"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO326_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5967542.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO326_003261"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO326_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5967543.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO326_003262"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO326_003262"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5967544.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO326_003263"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO326_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5967545.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO326_003264"
     CDS             3646..4155
                     /locus_tag="OO326_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5967546.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO326_003265"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO326_003265"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5967547.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO326_003266"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO326_003266"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5967548.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO326_003267"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO326_003267"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5967549.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO326_003268"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO326_003268"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5967550.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO326_003269"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO326_003269"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5967551.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO326_003270"
     CDS             complement(8797..9690)
                     /locus_tag="OO326_003270"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5967552.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO326_003271"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO326_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO326_003272"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO326_003272"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5967553.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO326_003273"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO326_003273"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5967554.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTRW010000104        12055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRW010000104 DAJTRW010000000
VERSION     DAJTRW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(400..1044)
                     /gene="cheZ"
                     /locus_tag="OO326_003274"
     CDS             complement(400..1044)
                     /gene="cheZ"
                     /locus_tag="OO326_003274"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5967555.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1055..1444)
                     /gene="cheY"
                     /locus_tag="OO326_003275"
     CDS             complement(1055..1444)
                     /gene="cheY"
                     /locus_tag="OO326_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5967556.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1459..2508)
                     /gene="cheB"
                     /locus_tag="OO326_003276"
     CDS             complement(1459..2508)
                     /gene="cheB"
                     /locus_tag="OO326_003276"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5967557.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2511..3371)
                     /gene="cheR"
                     /locus_tag="OO326_003277"
     CDS             complement(2511..3371)
                     /gene="cheR"
                     /locus_tag="OO326_003277"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5967558.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3390..4991)
                     /gene="tap"
                     /locus_tag="OO326_003278"
     CDS             complement(3390..4991)
                     /gene="tap"
                     /locus_tag="OO326_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5967559.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5037..6698)
                     /gene="tar"
                     /locus_tag="OO326_003279"
     CDS             complement(5037..6698)
                     /gene="tar"
                     /locus_tag="OO326_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5967560.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6843..7346)
                     /gene="cheW"
                     /locus_tag="OO326_003280"
     CDS             complement(6843..7346)
                     /gene="cheW"
                     /locus_tag="OO326_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5967561.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7367..9331)
                     /gene="cheA"
                     /locus_tag="OO326_003281"
     CDS             complement(7367..9331)
                     /gene="cheA"
                     /locus_tag="OO326_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5967562.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9336..10262)
                     /gene="motB"
                     /locus_tag="OO326_003282"
     CDS             complement(9336..10262)
                     /gene="motB"
                     /locus_tag="OO326_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5967563.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10259..11146)
                     /gene="motA"
                     /locus_tag="OO326_003283"
     CDS             complement(10259..11146)
                     /gene="motA"
                     /locus_tag="OO326_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5967564.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11273..11851)
                     /gene="flhC"
                     /locus_tag="OO326_003284"
     CDS             complement(11273..11851)
                     /gene="flhC"
                     /locus_tag="OO326_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5967565.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11854..>12055)
                     /locus_tag="OO326_003285"
     CDS             complement(11854..>12055)
                     /locus_tag="OO326_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5967566.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2766 a         3377 c         2920 g         2992 t
ORIGIN      
        1 agtaaagata attacttgac cgacttaaac cgcagacatc ttgaacttgt tcttcatact
       61 aaccccaatc caagcggttc ggtaacgaag attttacgca ctatttttac taagcaggta
      121 aagagtttag ggccggaact ctgtcatttc ttgcaccatg aactgacgaa aaaacgttat
      181 gaagtatggc atcactacga tgtactgcac taccagtcat cgtcattttc tcgttcacaa
      241 tttaatgcat tgataaattt actggcgcaa caatcacgct tcgctgatct aaaattatca
      301 aactacaaat aaccatcctc cacgtactcc ctgaacgata cccgccaccc tgctatcggc
      361 ttggcatcat cgcgcaggcc gaagttcagg caatacaaat caaaatccaa gactatccaa
      421 caaatcgtcc acctgatcct gactggctac cacgccggct ttgctggtat cgacctgagg
      481 tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc
      541 caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat
      601 aacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt
      661 agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg ttaccagttc
      721 acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc
      781 ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc
      841 ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg
      901 gatggcttcc gccgcttcgg caatggcctg aaccagcccc agttcccgca aactgtcgcg
      961 cagcatatgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc
     1021 aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt
     1081 tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc
     1141 ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg
     1201 gcaatgccga catcgcgcca tccgcacgaa ttgttttcag caattccagg ccatccatat
     1261 tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag
     1321 cgtcgacacc atcttccgct tcctcaacat tattgaatcc cagctctttc agcaggttac
     1381 gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg
     1441 ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattg ttgccaacat
     1501 ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc
     1561 gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc
     1621 ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc
     1681 aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta ccgaaggccg
     1741 atgacggtta accgccgggc cgtcgtgaat tttgatttga taatttgcgc cactacgcgc
     1801 cagttccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga cacgttctcc
     1861 gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg
     1921 ggtgaaaccg ggcggcatat gttgggtaat taacaatgcc gggctggaaa gcggcaacgg
     1981 ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt
     2041 ttcagaactc aacaacggcc ctgccttcag cgttgtcggt gctgacaatg gcttatgtgc
     2101 tgcaaggctc gcctttgctg ccgtacgcac cttttcagca atcatttcgc tatacgccag
     2161 cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctattgccc ccagctccag
     2221 cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa gaaaccatca caacgggcat
     2281 tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac
     2341 atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcaa ccagcggatc
     2401 aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg
     2461 gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta
     2521 cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcgaggtg gctaaagttt
     2581 tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa
     2641 atctcctgct gggtagtttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca
     2701 aacggccccg gcacggtgta ctgtttcgcc agtaaattca gcggggcaaa atcaacatag
     2761 ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa
     2821 taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata gataccgctt
     2881 ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc
     2941 acggtgccca atgtgtcagc cagagtcatc gcaatgctgt acggctcttc gccggtcgaa
     3001 gccgccgcgc tccatacgcg atcctcgcca gaacggcgac gtgcgtgatc cgcgagcaga
     3061 gggaaatgat gagcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac
     3121 gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc
     3181 gtcagtccca gagaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg
     3241 tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg
     3301 tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga
     3361 gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc
     3421 gccgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa
     3481 agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag
     3541 gagttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg ctcaatcccc
     3601 cgccgttgtt cttccgacgc cgaagcaatt tctcccataa tgtcgttcac gcgggtcacc
     3661 gaactgacaa tatcggtcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg
     3721 acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg cgcgctacgg
     3781 ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg ctctccggcg
     3841 cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc aattccgtcg
     3901 ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg catggtgtga
     3961 gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt
     4021 tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata
     4081 ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcgtta
     4141 ttgcctgcga caatctccgc gatcccaatg tgcatctcat ggcttccctt acgcacatca
     4201 ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc tggcaaaaat ggcggtgatc
     4261 tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa
     4321 tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg
     4381 ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagcgccga aatctgatag
     4441 ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccag ctgccaggcc
     4501 tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa
     4561 agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa
     4621 cttttctccg tctctttttg caatgccctg acgtgctcgt tgcccgcagt catcgccata
     4681 aaacttttaa acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt
     4741 ttaatgtcat ccgccggata gctaagcgct gtcagagtgc ctgctttgtt cagcgcggta
     4801 ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc
     4861 tcgccgtgat tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg
     4921 ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata
     4981 cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa aactcgtcaa
     5041 aatgtttccc agtttggatc ttgttcagta attcgcagtc gtggctgtgc cggtggttgc
     5101 tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacgg
     5161 aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc tgcggcggca
     5221 gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc
     5281 aatgcgactt gatcgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata
     5341 atgtcagtca cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg
     5401 accagcaccg aaccggtatc aatgcgcgag acggagtctt caatgagggc tttgatctct
     5461 tttgccgcca gggcgctgcg actggcaaga ttacgcactt cacccgccac cacggcaaaa
     5521 ccacggccct gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc gaggatatta
     5581 gtctggaagg caataccgtc gataacgctg ataatgtcgg caattttctt cgaactatcg
     5641 gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg
     5701 tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact
     5761 gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttca
     5821 gtacgggagg aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca
     5881 tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg
     5941 ctctgcgcca ggtcacccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta
     6001 ccaccggcga tttcgcaaat gtgagcaata atttttgcca gcggagtaag caacatacgg
     6061 cgaatgccgt accacgccac cagcagaatc aataccacca ccagcgcgat aaccgccagt
     6121 tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt
     6181 tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt
     6241 ggctgagcga aataagctcc agtattgcca taatcaagat aatcaatcag ttcagttaac
     6301 gctgtgtaat agtttttata tttttcatca aaattacggc tggtagcgac catttcaggt
     6361 aacggtgcca tgcttttgaa ttttttataa tgcgtcgcgg cctgggccaa tgttttcctg
     6421 gcgctatcga gcaattcaac tttggcgtta ctttgttgat tggaggaatc catcatcatc
     6481 cgcaccgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc
     6541 agctcgccct gctgttcccg taattgattg gaaaccacaa agctcttctg gctatggtga
     6601 agggaagaaa aaaacaaact gccggaaata agctgtaaca gtgcgaatac ccccagcacc
     6661 attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag
     6721 ttgatctcgt tatcggcaag aaggggggaa actttattgc tgatgccacc cgccgcgaaa
     6781 ttgaaataaa aaacccgatg cgcagatcat cgggttcatt tcaattgagg aaatcgggag
     6841 aattacgcca cttctgacgc cgcgctatct aacagcgcca tctcttcgct gttcagcagt
     6901 ttttcgatgt tcaccagaat caacatacgg tcgcccagtg cgcccagtcc agtgagatat
     6961 tctgttgaaa gcgtcacggc aaactctggt gccggacgaa tttgctccgc cgtcaatgaa
     7021 agcacgtctg aaacgccgtc aaccacgatg ccgaccaccc gctgtccgag attcaggacg
     7081 ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtca
     7141 acaatcggca caataacgcc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc
     7201 gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg
     7261 tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc
     7321 tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt
     7381 tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca
     7441 cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt
     7501 caaggttttt aaccacaacc tggtgttgac caattaattg atccaccagc aaggcgtagc
     7561 ggcgaccgcc actttgtaag atcaccacaa ttccctgggt ggcttcggtt ttcgcgcccg
     7621 cgacgttgaa cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca
     7681 cccgctcgcc gccggccagt ggatggagat cggcttcacg gggttgcagt gattccataa
     7741 cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga
     7801 tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga
     7861 tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc
     7921 gcccggagac gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat atcagcattg
     7981 caacttcgtc gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca
     8041 gaattcgctc acggtttagc cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc
     8101 cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt
     8161 tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga
     8221 taatgcgttc tatcaggctc ttgtcgagct cggtggaact gcccaccagc gtcagttcta
     8281 cctgcttgcc gagttttccc gccagatcac gcaccagccg gggatagcga ctaaagacat
     8341 attccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt
     8401 gtaactgccc catgctggta atcaaatcac catgattaac cgggtccagt tcgctggaac
     8461 gctgggcaag catggactgg gtgataacca gctcgccgac gaggttaatt aattgatcaa
     8521 ccttttctac cgctacacgg atgctggtgg attcactgct gcgcgtcgtt ttttcccgct
     8581 ccacgcggcc ggttggcgct tgttcggctg ccagtttaag cacaggtggg gtagctattt
     8641 ttggcgaaac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta
     8701 ccgctgtgat gtcatcttcg gcgatatcgc ccggtaatat tgccgagaga gaatccgccc
     8761 ctttcaccac gtcatttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt
     8821 cgccggcctt caggcgcgaa aggataattc gtcgcggcaa ctgactgcga ctttgctcat
     8881 cttgcggttc acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt
     8941 tcgcttctaa tgccagttga cgcaaggcct ggcagatata atcgtagctg gctgcatccg
     9001 gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa
     9061 acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttca tcgagcaggt
     9121 tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt
     9181 tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct
     9241 gcaaaaccag cagatgctgc tccatatcag ccaacagttc gtccgcttca tcaaaaaatg
     9301 tctgataaaa atcgcttata tcgatgctca cgctgtcacc tcggttcggc tgatggcatt
     9361 gtgggaacac tgacctgtgg tgcaacctca ggtttttcta gggcgcttac tggctcattc
     9421 tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccaacagg
     9481 ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc
     9541 atgccgacga cacgtaacac tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg
     9601 gatgcattgg cccgatcggc agaaagctcc cagttgctgt aacctttctc accgctggcg
     9661 tagggaaaat catcggtatg acctgaaagg ctaatacggt tgggaatacc gttcagcaca
     9721 ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga catcggcact gccggttcta
     9781 aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg
     9841 attttgagat ggagacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga
     9901 tcaccccgca atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc
     9961 ggctgcttat tcacttcccc ctggctttgg gtgtaatcat caccaccgcc gggaattggg
    10021 ctttcactat tggaaatgcg atcgccgccc gtaaccgctg tcgccagtgg agtccggaag
    10081 tactccgcaa tctgaatcaa ctcttttggg ctggagatgg agatcagcca catcaccaga
    10141 aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa tcttccacga tccatgtgct
    10201 gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc
    10261 atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca
    10321 gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga
    10381 taggcggtgc gtaaccgttc agattagaaa gcagagtgac tttgacgcac tgcatcattt
    10441 tgctggtttc ggcgcttttc tgacgtaaaa cactcgctag tggggaaata aatccgtaag
    10501 ccaataaaat accgaggaaa gtccccacca tcgcatgtgc gataagcgcc cccagctcgg
    10561 cggcaggacg atcggctgaa cctaacgcgt gaacgccccc cattacagcc gcgacaatac
    10621 caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcctcgc
    10681 tttcgtgcgt ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac
    10741 cgctgataat caggcgcaga taatcgacga taaaatcaag catgacgcta tccgcgagga
    10801 tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg
    10861 aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca
    10921 tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc
    10981 ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa
    11041 tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc
    11101 cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc
    11161 actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggaaaaaaa
    11221 aagcagcggt acgtcgttac cgctgctgga atgttgcgcc acaccgtatc agttaaacag
    11281 cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt
    11341 tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag
    11401 gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta
    11461 atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc agtggtcctt
    11521 cttctgcttg tgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc
    11581 cattacacaa accggttttc agtaaaaact gccatgcatt acagaacatc gaagcatgaa
    11641 cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg
    11701 ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc
    11761 tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc
    11821 gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc
    11881 ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg
    11941 ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa
    12001 acggaagtga caaaccagct gattggtttc tgccagctta accatttgcg gaaga
//
LOCUS       DAJTRW010000105        12039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRW010000105 DAJTRW010000000
VERSION     DAJTRW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..458)
                     /locus_tag="OO326_003286"
     CDS             complement(<1..458)
                     /locus_tag="OO326_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5967567.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTM"
     gene            978..1877
                     /locus_tag="OO326_003287"
     CDS             978..1877
                     /locus_tag="OO326_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5967568.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            1926..2939
                     /gene="yhjD"
                     /locus_tag="OO326_003288"
     CDS             1926..2939
                     /gene="yhjD"
                     /locus_tag="OO326_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5967569.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3350..4672
                     /locus_tag="OO326_003289"
     CDS             3350..4672
                     /locus_tag="OO326_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5967570.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4854..6914)
                     /locus_tag="OO326_003290"
     CDS             complement(4854..6914)
                     /locus_tag="OO326_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5967571.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(6984..7751)
                     /gene="pdeH"
                     /locus_tag="OO326_003291"
     CDS             complement(6984..7751)
                     /gene="pdeH"
                     /locus_tag="OO326_003291"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5967572.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7983..8912
                     /gene="kdgK"
                     /locus_tag="OO326_003292"
     CDS             7983..8912
                     /gene="kdgK"
                     /locus_tag="OO326_003292"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5967573.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9008..10504)
                     /locus_tag="OO326_003293"
     CDS             complement(9008..10504)
                     /locus_tag="OO326_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5967574.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10725..12011)
                     /gene="dctA"
                     /locus_tag="OO326_003294"
     CDS             complement(10725..12011)
                     /gene="dctA"
                     /locus_tag="OO326_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5967575.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2893 a         3270 c         2966 g         2910 t
ORIGIN      
        1 aacatggtta aaatctcccg ctggcgggcg cttaacgatt tgagatcttt aatgtccttt
       61 tccggcgtcg tccgccagtc tccaggcaga aacatcatcc ccatcgccgc actatttacc
      121 gccaacgcaa atgtctcgac ggttgaatcg cgaggcacaa tggccagcac attaaaatgg
      181 ataacttcct gtaaccaccg tttattgcaa tccgtcgccg tgattaacac cttaacctca
      241 ggaaactgca ccacggtttt ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca
      301 tcgagcataa ctaaggcttc agggtaactt tccagctttt gccataactc gtctgcctga
      361 ctgaccccct gaatactcac tcctggaata cgctgctgta aactgatttt tattccatga
      421 ataaatattg actgcctgtc aaacatgact atttgcataa ctgaatctcc acctgaatac
      481 gttaaaaaga cttaagtagt ggaagggtat tacccgcgag aaaaaataag aattcgccat
      541 ttggcggtgg ccattctaca gagatgacgc gtagaaaata gttaccgata taaatagtta
      601 cagctaaacg cctgaaatta catgtcgagg gcactattta aaacaatttt gaggatttcc
      661 ttatattggt ggttagtacg catgcaatta aaaatgaaat tccgcgacta caagccaaaa
      721 taacaaatgg caatgagaca aaaataagca caaatagcca atacgtcctc tattcacttt
      781 aaagggaatc actgaaaaat acgctctgtt taaggggatt cacctttctc ataaagctat
      841 tccgcccttt tcctgctgag aaatcgccac attcgtcatg acaacattgt gaaacccggc
      901 attagatgtt agaaaaaaca ataaacaatg cgatatgcgc gttactgttt cgctgtgaac
      961 aacaatcggt caaagaaatg gataaaattc acgcaatgca gttgttcatc aaagtcgcgg
     1021 agctggaaag tttttcccgc gcggcggatt tctttgcttt gccaaaggga agtgtttcgc
     1081 gccagataca ggcactggaa catcaacttg gcacccagct tctccagcgc actacgcgac
     1141 gggtcaaact cacgccagaa ggcatgacct attatcaacg ggcaaaagat gtgttgagta
     1201 atctcagcga actggacggt ctgtttcaac aggatgccac cagtatcagc ggtaaattac
     1261 gcatcgacat cccgtcagga atcgcgaaaa acctgttact gccgcgtctg tcggaatttc
     1321 tctatcagca tccgggaatt gagctggaac tgagtagcca taaccgtccg gtagatattc
     1381 ttcatgatgg ttttgattgc gtgatacgca ctggcgcgtt accggaagat ggcgttatcg
     1441 cccgtcccct cggcaaacta accatggtca actgtgccag tccgcactat ctgacgcgct
     1501 ttggttatcc gcaaagcccc gatgatctga cttcacacgc aatagtgcgt tatacaccgc
     1561 acctgggtgt acatccgtta ggttttgagg ttgccagcgt taatggcgtc cagtggttta
     1621 agtctggcgg catgttgacg gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg
     1681 gtctggggat tattcagatc ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc
     1741 ttattgaagt gttgccaggc taccgtgccg agccgctctc cctttcgctg gtttacccgc
     1801 agcgtcggga gctttcccgg cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga
     1861 aagagcacct ggactgaccg actatacttt ttaagaatga ccacgacaga aggacaaaag
     1921 agcggatgac gcaggaaaac gagatcaaac gtcccaccca ggatctggag cacgagccga
     1981 ttaagcagtt ggataatagc gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca
     2041 ccaccactgc cgaaaaagtc caacgccaac cggttattgc gcaccttatt cgcgcgacag
     2101 aacgctttaa cgatcggctg ggtaaccagt ttggtgcggc tatcacctat ttctcatttt
     2161 tgtcgatgat cccgattttg atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc
     2221 atccgatgtt gctacaggat atcttcgaca aaattctgca aaacatcagc gatccgacac
     2281 tagccgccac gttgaaaaac accatcaaca ccgccgttca gcagcgtacg actgtagggc
     2341 ttgtcggcct ggcggtggca ctttattccg gcatcaactg gatgggtaac ctgcgtgaag
     2401 cgattcgtgc ccagtcgcgc gatgtctggg aacgctcgcc gcaagatcag gagaagttct
     2461 gggtaaaata tctgcgtgat tttatttcac tgattggttt gttgattgcg ctgattgtga
     2521 cgctttcgat cacctcggtt gccggttcgg cgcagcaaat gattattagc gccctgcacc
     2581 tgaacagcat tgagtggctg aaaccgacgt ggcgattgat tggcctggcg atttccatct
     2641 tcgccaacta tctgcttttc ttctggatct tctggcggtt gccgcgccac cgtccgcgca
     2701 aaaaagccct gattcgcgga acatttctgg ctgctattgg ttttgaagtg attaaaatcg
     2761 tgatgaccta caccctgcca tcgctgatga aatccccctc tggcgcagca tttggttccg
     2821 tgctggggct gatggcattt ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt
     2881 ggattgccac cgccgaatat aaagacgacc cgcgaatgcc agggaaaacg cagccttaaa
     2941 ataggccgga tgcggcgtaa acgccttatc cggcatacga ttttattcgg cctggctccc
     3001 cgtaggccgg ataagatgcg ccagcatcgc atccggctat aatgcgcaca taacctcttg
     3061 aaactcttcc ccagagcctc ttcagccatc tatttgggag caaacaattt cactccaact
     3121 cataacccca gcatataaat ccagttggta acttttattt aacctgaaac cagttttatc
     3181 cactatttat aaaattatgt gaagcatttc atagaagaaa aatcactggc ctaaacatta
     3241 tccccttttt gcctggtttt tgaccatttc cgcgatttgt tacacattga aatatcactt
     3301 ttgctgtgcg taatatggct attcgttagc caaaaaataa gaaaagatta tgcaagcaac
     3361 agccacaaca ctcgaccacg aacaagaata cacgccgatc aactcgcgta ataaagtcct
     3421 tgtcgcctct ctcattggca cggccattga gttcttcgac ttttacattt acgccactgc
     3481 ggccgttatt gtgtttccgc atatcttctt cccgcagggc gatccaacgg cagcaacgct
     3541 acagtcgctc gccaccttcg ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt
     3601 tggtcatttt ggcgatcgcg ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat
     3661 ggggatttcg accgtggtga ttggtctgct gccgggctat gccacgattg gtattttcgc
     3721 cccactgctg ctggcgctgg ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg
     3781 cggcgcggcg ctgctggcga ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc
     3841 cttcccacag ctaggcgcac cgattggctt cttctttgcc aacggcactt tcttgctgct
     3901 ttcctggcta ctgaccgatg agcagtttat gagctggggc tggcgtgtgc catttatctt
     3961 ctcggcggtg ctggtcatta tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt
     4021 gtttgagaaa gtcgctaaag cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac
     4081 caaacatgtt cgcgtaaccg tactgggtac gttcattatg ctggcaacct atacgctgtt
     4141 ttacatcatg acggtctact ctatgacctt cagtaccgcc gccgcgccag ttgggcttgg
     4201 cctgccgcgt aacgaagtgt tgtggatgct gatgatggca gttattggtt ttggcgtgat
     4261 ggtgccagtc gccggattac tggctgatgc ctttggtcgc cgtaaaagca tggtgatcat
     4321 caccacgctg atcatcctgt tcgcgctgtt cgcctttaac ccgctgctcg gttctggcaa
     4381 cccgattctg gtttttgcct tcctgctgct ggggttaagt ctgatggggc tgaccttcgg
     4441 gccgatgggt gcgctgttac cagagctgtt tccgacagaa gtgcgttaca ccggagcatc
     4501 gttctcttac aacgtatcgt cgattctcgg ggcttccgtt gcgccgtata tcgcagcctg
     4561 gttgcaggct aactacgggt taggtgcggt ggggttatat ctggcggcga tggctggctt
     4621 gacgttaatc gccctgctgc tgacccatga gacgcgacat cagtcgttgt aataagtgtg
     4681 aaaagcctga tgcgctacgc ttatcaggac tgaatttcca tacaatatat tgaattcaca
     4741 aggatttgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcacactttg
     4801 ccaaaaatct aaatctgttt aattgccgga tgtcagacat ccggcaatta tcatcacttc
     4861 ttcatctgcg acaatatcgt ccgacactga ttagcctccc cttcggaagg tgagatcagt
     4921 gccagtaacg ccgccgccgg tgttaccagc gttgccagtg ccgcagcaac agcaccacgg
     4981 gcaatcagcg gtccggcttt caccccagcc tgcggatttt taaacgtccc acgcacatac
     5041 agcggcgaac gcagtgtgat aatccgaatc cctttactct ccggatcaat agtcaaatcc
     5101 agctgttccg aagcaaaact tgccgtgccg gtaacattaa tcaacgcgtt ctcagtatcg
     5161 aaagcaaaaa tctgcgggcg cgccacgccg ttggcaatat tcagattcgc cgccgcgcag
     5221 ttcacccgca cctcatcgtc accaaatatc gcaccgacaa tgtagttgcc gacattcagc
     5281 ccaacaatct ccatcaggtt gcggctcacc agcccgtcat tcatcaacag tttcaggttg
     5341 ccgttactgt tgcctaaaag cgccgccacc gagttaccgc taccgcgtag ttccgcgtca
     5401 ccgttcattt cccccagcgt cttctgcatc agttccacat cgggcatcag ttctttcagt
     5461 ttcagtcgac gagcctgaat atctgcccgc ccctgcatcg gctttttatc gccttccaga
     5521 tgaatattcg ccgcaatgct gccgcccgcc atgccaaatt tcagcggttg caggcgcagg
     5581 tcagcatttt tgaggatgat atgagtagaa agatcgctaa tcggcaggct actgccatgc
     5641 tcaatgcgcc gccctttgaa gcgaacatcg gcatccataa cgtcccattt atcggtttcg
     5701 aagcggtcat aaggcagcac tttgcccgca ggctgaacgc ttttttcgcc cttcttctgt
     5761 tcagaccgtt tcgacttttc tgcccctttc ccggaatcaa cgccaatcaa cggtcccagg
     5821 tccgccagcc gcaattgccg cgactcgaca tcaccttcca gttttggtcg tggctttccg
     5881 gtggtgtaga ccagagaacc gtggatatcg ctatcgccaa ttcgcccatt aaaaccgcga
     5941 taatcaaaga ccgacgattt ttcagtgtcg attttcgcta ccagccgacc atccgtttca
     6001 aacggcgggg tatcgggcag cagaacgccc gtcagttcat agagatcacc cagtgaatcg
     6061 ccagaaaatt taagccgtaa atcgacaccg cccatcttca ttgggtcatt cacgacgcca
     6121 tcaaaagcaa cacgggtatt acctgaacgg aaatcagcct gtaccggaaa cggcgtccct
     6181 tcgccacgca gcgccagcat accgcctatt tttcccgtac ccgtgagcgg ttcaccgtta
     6241 taacgtccct gcgccttcag gccaaaaatg taatcgccca ccttttcttt atccgcttta
     6301 cctttcgatc cagtaacttc gctgaatggc agcggcttgc ctaacggatc aacaaaaatt
     6361 tccagatccg ctttgcttac tttgtcatca atggcgatcc gcccttgatc gaaaagaata
     6421 ttatccagcc gaaacgacca tgccgacggc tttgcattcg cgtctttgtt gtcatcgttg
     6481 gcaagattaa acgtccagtt attgttcttt tcagagaggc gaatcaggcg cgcgtcgggc
     6541 ttttcgagct tgatccacgg cagccagacc gttttggtca gcagcgccag cggtgccagc
     6601 gttgcctcta ctcgcggcaa atgcaccatc gtgacttcgg gaatatccgg tggattacca
     6661 agaatgatgt cttctgcatg tacatgaggc cacggcaccc agctgcgcca gccagtttct
     6721 tgtttttgcc gctcccacac cacgcccaga ttgccacgga tagcgaacgg acgattcaac
     6781 tccgcagaga ctttctggtt gatggtcggt ttgagtcgat tccagtcaaa agttgcaatc
     6841 aaaatgatcg ccacgacaat caacaacaag aaagcccctg aaatcgcagc ggttattttg
     6901 cctgccttgc tcatcgttcg cttcctcata ttcttcctgt gccagtccta aagatagtcc
     6961 agcccggcgg aaaatgaggc agcttatagc gccagaaccg ccgtattcag cgtttccatc
     7021 ggtgccgggc gtgaaagaaa ccagccttgt gcggcgaatg cgggcgagtt ctgaacatca
     7081 cgccactctt ccggtgtttc tacgccctcg acaatcaccc cgcgacaata gcgattcatt
     7141 agatgtaaaa gctgagaaaa gagtgtgcgg ccttccggcg actgacgcag catcacaaac
     7201 agttctcgcg cgattttgat gtagtcataa cgcacttcac ttagtgcaga gaaatttgcc
     7261 atcccggtac caaaatcatc cagccacagc gggccaaatt cacacatcga ggcaaaggtt
     7321 gaatctttcg gcagacggat atgctccacc agttcgaaac gcagccaggg aagacgctca
     7381 atctggcgca ggatttttgg ttgctgacgc agggcgatga gcgtagggcc atcaatattg
     7441 accgatgcca gcaggccgtg ctctacaaag aagtcggctt tttgcgccag caaatcaatc
     7501 tgctctttca caacctccat ccgatggctg acggtgattt cagtaaaata gcgatccggc
     7561 ggcaggcgtt gcgaagggtt caagggatgc gtgaccaccg ttaatagctc cacggccatt
     7621 aaccgcccgc atgtttgata gatcggctgc caggtgtaag cacgctcaca ctgcaaccaa
     7681 aaacgccgtt cctgcaagct ctcgatgctt gcttcagggt tgcttattcg ctggataacc
     7741 tgccttatca tcaaagatgt cctgtgttta aaagtgatgc tgcccggact cgacaaagat
     7801 tatcggcgcg taagggcaga actttaccgc ctggccgggg taaatttcct cactccacaa
     7861 cggaatcttt caggaatacg ttctggctca aaaaaataat ggaacactgt tttaatatgg
     7921 ttgaccagca aaccaccaca gcgcaaacta acgctaattt tttacagatc aggttcacga
     7981 ctatgtccaa aaagattgcc gtgattggcg aatgcatgat tgagctttcc gagaaaggcg
     8041 cggacgttaa gcgcggtttc ggcggcgata ccctgaacac ttccgtctat atcgcccgtc
     8101 aggtcgatcc tgcggcatta accgttcatt acgtaacggc gctgggaacg gacagtttta
     8161 gccagcagat gctggacgcc tggcacggcg agaacgttga tacttccctg acccaacgga
     8221 tggaaaaccg tcttccgggc ctttactaca ttgaaaccga cagcaccggc gaacgtacgt
     8281 tctactactg gcgtaacgaa gccgccgcca aattctggct ggcgagcgag cagtctgcgg
     8341 cgatttgcga agagctggcg aatttcgatt atctctacct gagcgggatt agcctggcga
     8401 tcttaagccc gaccagccgc gaaaagctgc tttccctgct gcgcgaatgc cgcgccaaag
     8461 gcggaaaagt gattttcgac aataactatc gtccgcgcct gtgggccagc aaagaagaga
     8521 cacagcaggt gtaccagcaa atgctggaat gcacggatat cgccttcctg acgctggacg
     8581 acgaagacgc gctgtggggt caacagccgg tggaagacgt cattgcgcgc acccataacg
     8641 cgggcgtgaa agaagtggtg gtgaaacgcg gggcggattc ttgcctggtg tccattgctg
     8701 gtgaagcgtt agttgatgtt ccggcggtga aactgccgaa agaaaaagtg atcgatacca
     8761 ccgcagctgg cgactctttc agcgccggtt atctggcggt acgtttgacg ggcggcagtg
     8821 cggaaaacgc ggcgaaacgc gggcacctga ccgcaagtac cgttattcag tatcgcggcg
     8881 cgattatccc gcgtgaggcg atgccagcgt aaacgcagca tatgcaaaag gctggataaa
     8941 gtgttacccc tctatccagc tcatccataa atacctgatg gttgtgctaa ctcatcacca
     9001 tcaggcatta ctgtgcaggt ggaatatccg tcacttcagg atgtacgtca tccgtggcga
     9061 cagaggtggt cgcagcggcg gtagatgggg ccatgatttg atcccaggcc gcctgcaacg
     9121 ctttcatgtt aaattccggc tcgcctttcg gctgcagcag tattaacgcc atatcattcg
     9181 acaactgctg acgcagatcc tgatttaaca tctccacagt caggctgtta aggaaatcct
     9241 gccgcagttt ctgatactgc tccggcgcga tatcgacgac ctgattttgc aacgaacgca
     9301 tccgctggcc catcagaata tcggtatcag cccgtgcata ggcggcaaac agtttctgca
     9361 gctccagttt cttttgcgcc actaacgcat tgaactcttc ttccggcaga cctttatcgc
     9421 gaaccttcgc cagttcacgc gccaccagat taaggttgct gttcagcttg tcgtttggtg
     9481 attcgatgtt gatggcacac tgcgcacgca gatacagcac acggcagtca aatccaagac
     9541 cgatgtcttt gctgtcactg gcgcttaacg cttgctgaac atgccagaac agcgcctcgc
     9601 gggccaggtc cgcacgccag tagcgcagca gtgcggctga ttcacgaatc ggctgccacg
     9661 gcgtatccca catgatagat aaccggtcct gacgcaccgc gtcagtcata atactcaccg
     9721 cttccgcacg cagcggagaa agcgtcggca ccggagctgg cgtttcacgt ttgcctttca
     9781 gttcgccaaa cgttttgttg atttggtcga caaccgagcg cgcatccacg tttcccacca
     9841 ccagtagcgt cattgcatcc ggggtgtacc atttctgata gaaatcttta attttttccg
     9901 cttcaacggg ttgtttcagc ggatcggcag gatcgtgacc taacaaggtt gaccctttca
     9961 gacgatagcg ccaccagccc tctttagtat cggcaggcca ggttgccacc atgtcctgac
    10021 tttgcagcgc gtggttgatg gtttctggtg tgatggtcaa tttgccagtg gcatttgcca
    10081 gataagagag cgcttctttc agcaaatcgt tacggttatt gggcaaactc agattaaaca
    10141 gcgtggtgtc ataagagaca attaccggcg gcatcgggcg tttagggtcg atcccctgct
    10201 gccacaatga acgcgcctgt gctgcgtcaa ggccaccgct ttgcgttagc gcaatacgag
    10261 ggatggcgtg actgtaaccg ctctgttgtg tactttcggc gagcgaaccg gtattaacca
    10321 gcaggcgaat ttcaacacga tcgctgggac gctggggggt ggtcagcact tgccactgta
    10381 aaccgttgga aagcgtcccc tgttgccatg ctggatcagg ctggagcgca tctgcctgca
    10441 catagccagc agtggccatc atcagcaaac cgcccgctaa aagtcgaatt tttatgccct
    10501 gcatgtgaac ccctgatcaa ctatcctggt aataaaaaaa ctgcccgttg tcggtcagtc
    10561 taaatgacgt taaaaacact tcgtgttaga ccgcaagaac atgaaaatgt cacggaagaa
    10621 gtgaaataaa cctgaactca cccaggtggt aagttcaggc acaggggtca attatgcgca
    10681 aacacccgca ctcggggaag ggagtgcggg cataagtggt gagattaaga ggataattcg
    10741 tgcgttttgc catccggcgc acgattattc agcacatcgt ccagtttttt gtggtccagt
    10801 tctttcaccc acttagcaac gacaatggtc gctacgccgt taccgaccag gttagttagc
    10861 gcacgagctt ctgacataaa gcggtcgata ccgaggatca gcgccagacc cgctaccggc
    10921 aaatggccca ccgcagagag cgtcgccgcc agcacgataa agccactacc cgttacccct
    10981 gccgcccctt tagaagaaag cagcaacacg attaacagcg tgatttggtg gacgatatcc
    11041 atctgactgt tagtggcctg ggcgataaac accgccgcca ttgtcaggta tatcgatgtg
    11101 ccatcaaggt taaacgagta gcctgtcggg atgaccagcc ccaccaccga tttacggcag
    11161 ccgagtttct ccatcttgtc gagcatacgc ggcagcgccg actcggaaga tgaagtcccc
    11221 agtacaatca gcagttcttc acggatgtag cggataaatt tgaagatact gaaaccagtc
    11281 gctttagcga ttgaacccaa taccagcacc acaaacagga tacaggtaat gtagaaacag
    11341 ataatcagct gccccagttg caccagtgtg ccgacgccgt atttaccgat ggtaaacgcc
    11401 attgccccga gcgcaccaat aggtgccaga cgcatgatca tattgatgat gccgaagatg
    11461 acctgcgaga aactttcgat cacgttaaaa atcagttggc ctttgctgcc cagacggtgg
    11521 agcgcaaaac caaacagtac ggcaaacagc agcacctgca gaatgttacc gctggcaaat
    11581 gcgccaatga cgctcgccgg gatgacatcc ataatgaagg cgacaatgcc ctggtctttc
    11641 gcctgatcgg cgtaaaccgc taccgctttc gcatcaagcg ttgccggatc gacgttcatt
    11701 ccggcaccag gctgcacgac gttaacgatg ataagaccaa taatcagcgc gatggtactg
    11761 acaatttcaa agtaaagcag tgcgactgcg ccggtacgac cgaccgcctt catgctttcc
    11821 atgcccgcaa tgcccgttac gacggtacaa aagatgacag gagcgatgat catcttaatg
    11881 agcttaacga agccgtcgcc aagcggtttc atttgctcgc ctatttcagg atagaaatgg
    11941 ccaaggagaa taccaatggc tatcgctgtc aggacctgaa agtaaaggct tttaaacaga
    12001 gaggttttca tagggtgtcc tttagtaaaa ccacaggtc
//
LOCUS       DAJTRW010000106        11991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRW010000106 DAJTRW010000000
VERSION     DAJTRW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO326_003295"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO326_003295"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO326_003296"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO326_003296"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5967576.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO326_003297"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO326_003297"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5967577.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO326_003298"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO326_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO326_003299"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO326_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5967578.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO326_003300"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO326_003300"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5967579.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO326_003301"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO326_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5967580.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO326_003302"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO326_003302"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5967581.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO326_003303"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO326_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5967582.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO326_003304"
     CDS             9553..10332
                     /locus_tag="OO326_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5967583.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO326_003305"
     CDS             10322..11131
                     /locus_tag="OO326_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5967584.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO326_003306"
     CDS             11218..11652
                     /locus_tag="OO326_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5967585.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>11991
                     /locus_tag="OO326_003307"
     CDS             11649..>11991
                     /locus_tag="OO326_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5967586.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGG"
BASE COUNT         2863 a         3233 c         3054 g         2841 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga c
//
LOCUS       DAJTRW010000107        11982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRW010000107 DAJTRW010000000
VERSION     DAJTRW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OO326_003308"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OO326_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5967587.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OO326_003309"
     CDS             complement(2051..2974)
                     /locus_tag="OO326_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5967588.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="OO326_003310"
     CDS             3191..4534
                     /locus_tag="OO326_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5967589.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OO326_003311"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OO326_003311"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5967590.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OO326_003312"
     CDS             6554..6778
                     /locus_tag="OO326_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5967591.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OO326_003313"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OO326_003313"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5967592.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OO326_003314"
     CDS             complement(7372..8352)
                     /locus_tag="OO326_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967593.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OO326_003315"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OO326_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5967594.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OO326_003316"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OO326_003316"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5967595.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11017
                     /locus_tag="OO326_003317"
     CDS             10361..11017
                     /locus_tag="OO326_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5967596.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OO326_003318"
                     /pseudo
     CDS             complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OO326_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11549..11982
                     /locus_tag="OO326_003319"
                     /pseudo
     CDS             11549..11982
                     /locus_tag="OO326_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3106 a         2800 c         3007 g         3069 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
    11521 tttcacagac caaagtatca tttgacctgt gaaacgatta caggcattta aattccagtt
    11581 aagaccaggt ggtcaacagg agtgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
    11641 tttcaatcgt gctctggcac gtcagaatga gaatcataag gccggtaata aatacatccc
    11701 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
    11761 taaagattct ccctcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
    11821 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggtgac agaatgatgc
    11881 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
    11941 actgggctgg atgcgctacc ggaatagccg tcaggtcacg gg
//
LOCUS       DAJTRW010000108        11816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRW010000108 DAJTRW010000000
VERSION     DAJTRW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..2233)
                     /locus_tag="OO326_003320"
     CDS             complement(<1..2233)
                     /locus_tag="OO326_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5967597.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNG"
     gene            complement(2167..2790)
                     /locus_tag="OO326_003321"
     CDS             complement(2167..2790)
                     /locus_tag="OO326_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5967598.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2787..4475)
                     /locus_tag="OO326_003322"
                     /pseudo
     CDS             complement(2787..4475)
                     /locus_tag="OO326_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4624..7251)
                     /gene="gyrA"
                     /locus_tag="OO326_003323"
     CDS             complement(4624..7251)
                     /gene="gyrA"
                     /locus_tag="OO326_003323"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5967599.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7398..8120
                     /locus_tag="OO326_003324"
     CDS             7398..8120
                     /locus_tag="OO326_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5967600.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8248..10182)
                     /gene="yfaL"
                     /locus_tag="OO326_003325"
     CDS             complement(8248..10182)
                     /gene="yfaL"
                     /locus_tag="OO326_003325"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5967601.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10192..11787)
                     /locus_tag="OO326_003326"
     CDS             complement(10192..11787)
                     /locus_tag="OO326_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5967602.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2731 a         3282 c         2919 g         2884 t
ORIGIN      
        1 gaccattacg ggtatacagc accgaaaaag tgatgttagg cgcaaaggaa ttgctcactg
       61 gtacccgggc ttcatacagg gtatcgttta aacgttgtag cgttagccag tttgccggat
      121 gcgaaagcag cgactgctgt tccacgcgat cgcgttccag cgtcaataac gcttcatcaa
      181 ttggctccgg gaaagtaatc agcatcttcg cggtttcgcc tggctggtac agcgttttat
      241 ccgccacgat atctaccgta ccagtatgcg ccgtgctgcc cttaccgctg acggcatgac
      301 ttaacccagc gagaattaag ccgtctttat cgcgtaatgt cagattgtag ttgccaggtt
      361 tagcgaaatt gacggtaaag gatttgccgc ctgacggtag ctctccgcta tggctcgtgc
      421 ggtcttcgag acgcaaccat tcatacgtaa caggaacctg ttttgaagat tccagcgcgg
      481 cataacggaa cacaaccgac tcgccgctat tactgtattg tgcggcggta cttaaagagt
      541 aatgcgccag gccgcgttca atgaggatct ctttggtggt ggtgacgcga tacgccgcgc
      601 cgtcactggc ggagacggtt aacaaatagc ggctcggttt atcggcggcg gggagattta
      661 acgccacatg accgctggcg tcggacaccg tttcgctgcc ttccagcgac acggggaaac
      721 gtccggcata acgcaaatcg ttaccgacca ttgataattg ctgagcgcgc aaacttaact
      781 gcacgcgggc attttttacc ggctcgccat ccgggtagag cagttgcagt ttgccgctga
      841 ccgcttcgcc agttttgaac tcttttttgt cgagagctaa accaatctcg aaatgtggct
      901 tgatgtagtt tgccacgcga aaactgctgc tatagacctg attgcggtaa gcaagacgta
      961 actcataacc tccggcaacg gcattttctg gcaggcggaa acttccctgc ccgccattgc
     1021 gcgcatccag cgtgacatcg acggtttgca acagactgcc gttagcgtcc agcaccgaaa
     1081 gcttcgccgg ggcgctgacg atgggggatg aatgcaacgg atcgcggaac tcgcggccca
     1141 tcactttaac atcgacacga tcgcctgcgc gatatagcgg gcgatcggta aaaatataca
     1201 agcgggtgtt gtagatttcg ctttcgtaga agaagttctc ggagacaaaa acgccgcctt
     1261 cagcatcctt acccagaatg tatgaacgtt ctggcgatat atgttgtaac tgcaaagtac
     1321 cgctgtcatc ggtcaccccg cgggtcatca cgccaagacc ttcagtccac aagatctctg
     1381 agccgggctt cgcttcaccc tgttttttac ccgcggtcca taccagaagc tcgttacctg
     1441 acactttgct aagcgccacg gtatcggaaa caaacaccac cgtcgtcgcc cgatacccac
     1501 ctaccatcgc ctcgacgagg tacagccccg gctcttgttg gccgagagga atataaatat
     1561 tacccggctg cggcgagatg aaattgctgg atgcgccttc cagttttacc ccttgctgcg
     1621 gctcgaccgg tttagcctgc catagtggat aacggaactg ttccaccaag ggatattttt
     1681 tcagcgggga aaactggttg ttctgtacat aacgggaagg tttaataatg gcattgccga
     1741 gatgtaattc gggtaatgcc tgagtcacat tctgccgtga ctgagaagag aaagtacgct
     1801 gcatcacgcg gcgagatttg ccgtaccagt tatcccacag ccaggtcagc gtattgttca
     1861 gcccgtcgcc cagatattgc ggttgcacca caatgcgatg caggtttttc tgctggcgca
     1921 aaaatgccat cgggtcagga atacgatata ggcgaacgtc cacgccgccg tactcttcca
     1981 tctgatagcg ccgataatca cgccccggcg cttccagtcg cactttcgcc tcttcactgc
     2041 tgctaaaact gctgtcagca agcaaaaaga atgttccccc ggcgggcggc gcatagttac
     2101 tggaaggaag cgaatcatca gctttagcaa gccctgttcc cacaagagac agggacaagc
     2161 acgctgctat cgcgccagaa aatttaaacg ataaatgcca atgaaattgg gattggaatc
     2221 gttgggtatc catcgggtgt ccttccatgt cataagttgt tgcagactga ctgcgcgcat
     2281 tccgttgtca gttttcgtgg cgcttccggt gtggtagatg acgtaacgcc ccatccagac
     2341 cattaagtgc tgggcatcgc cctgatcgaa aaaaatcata tcgccaggca gcgcctggtt
     2401 tatgtcttgg ccaataaact ggctgttgta ctgaatcaaa ttaatcgcgg tcacgtaggg
     2461 gccgtttttc ccgctcccct gattccagtt ttgcgccagt tgacgctgtt caggtgttag
     2521 cgtcatctct ggcggcaaat actggctaga taaaccgtta ctttttagcc atttactgtc
     2581 gtgaactttc agcgtctcgt tcgccgcaaa tcgcaccagg cccgcacaat cctgctgata
     2641 ccagcgtgga cttggcccct ggcggagctg ttcttgtgca atgcgtacaa accaggcgcg
     2701 aaacagcctg gattgttcga cattcagcat ttcgctatgg gcaacaacac aacacagcca
     2761 gcaaatcagc gccagcagcc cgtgtctcat agcggctgcc aggttatggg tagccactgc
     2821 caggcgacac cgggttccat ctgggccagc ttcatgacat aacgcggttg ttgagataaa
     2881 gcgtccagct tcggcattaa taaagtttgt gcggcgttat aaaaaaccgg ttcgagattc
     2941 ttcggcagac tggtcaacgt ttcgttacgc agcagtttcg ccacgccttg tggattgata
     3001 tagagcggaa cgatgccatc agtgggtatt acatccacca ttgccgggcg ggttttattc
     3061 agtgtttgca gggcgttatt aaccagcgtg tcatcgaggg agaaaagcag cgttttgttt
     3121 tgcatcgcca gcgacacacg gaaaaaataa tccgacatta attgatcggg ttgcgccgcc
     3181 tgcgctttcg gatactgacc gtatcgggaa ctcacttcgc gacaccagat ttgcgcttcg
     3241 ccctgctgag tctggcttac cggcaaaaca ccttctggcg ctttgctttc gtgcgcacca
     3301 atattttgcg taaacagttt ccctggcaat tgcgcctgtt cggcagtgcc atcaaactga
     3361 ccgacaaaca gcggggtttg caattttgag tcttcatacc agcacagccc cgcggcaccg
     3421 tctaacgccc cattcagctt gtcgttttcc tggctgatgt gcgaaagcat ctcttcggca
     3481 ataccgtgtg aatacggcac cgccacacag aagctggctc cggcaggcat actgttccag
     3541 accggcgtaa aatcgaaact ggcatctacg ctggcggatt catcatttaa cgccacaaaa
     3601 ctgtgccagc cgtcgttacc catttcgaag cgtacgccag caaaagaagg cattaaccgc
     3661 tggtagccaa aacccagcaa cctggcgctg accacgatgc gcaggcgtac tggcgttttt
     3721 tcggctgtac gctcttccag gccaaagctg gcttgccagc gttttttacc gctcaacaaa
     3781 tcacttgcga tcgccgtggc ttcggtatcc tgctgatcat ctttaaacaa catatccgta
     3841 ctggaaaaca ccagcatctt gtcctgatat gtcgcgaaca tcagggcgtt attgccgtta
     3901 tagcgcaact gataaacagg aatagtttca ctatttatct tgatgctact gatttccgtt
     3961 ttgcttaact agctgtcgct ggtagcggca aacaacaatg gctccagcag tttgcttaaa
     4021 ccactgcgct ggatcagcac catgtaatgt gaaagatggc cttgtttatc gtgccatagc
     4081 gccgcctgcg cgggctgatc taagagtgac gaaaagagct tatctttcag cgtgagatcg
     4141 tgttcataga caatgcggcg aatgctgcct tcaatgccca gacgatcggc atgattctga
     4201 taatagaaaa cgaaatcttc gctcagaaca tcgtggagaa acggaatggt gaggagatct
     4261 ttgggaagct ggctcagaga gtcgctgtct agaaagaggt ccggctcatt gagatcgatt
     4321 tgcagattgt tgtgcaccac cagcggcgac aacgtttttt ctggcccact gccagcatat
     4381 tgcaacgccc agacgccagc ggaaagcagt gctattgcgc caaaacctac aagaccatag
     4441 aaccgccagc ctttcgcctt tttttcacca ctcattgcca cattccttgt gtatagccag
     4501 ccatttttta cgggcacagc caaactttac cgcgccctaa tacgacaaaa gcccagactt
     4561 tgcagcctgg acttttcaat tcaaacaagg gagatagctc ccttttggca tgaagaagta
     4621 aaattattct tcttctggct cgtcgtcaac gtccacttcc ggagcaattt catcatcccc
     4681 ttccgcggca ctgccgtcga tggtatccag atcttcctcg tcaaccggtt cagcaacacg
     4741 ctgcagaccc actacgtttt catcttccgc agtacggatg aggatcacgc cctgggtgtt
     4801 acggcccacg atgctgattt ctgaaacgcg agtacgtacc agcgtaccgg catcggtgat
     4861 catcatgatc tggtcgcagt catctacctg caccgcgcca accactaaac cgttacgttc
     4921 ggtaaccttg atggagataa cccctttcgt cgcacgcgac ttggttgggt attccgccac
     4981 tgcagtacgt ttaccgtaac cgttttgcgt tgcggtgagg attgcgccat cgccacgagg
     5041 cacgatcaga gagacgactt tatcgccttc acctaagcga ataccgcgca caccagtggt
     5101 attgcagccc atcgcacgga cagaagactc tttaaagcgc accactttac cttcggcgga
     5161 gaacagcatt acttcatctt cgccactggt cagatcaacg ccgatcagct catcgccgtc
     5221 aaccagtttg atcgccactt taccggcggt acgcagacgg ttgaattcgg tgaggacggt
     5281 tttcttcaca gtaccgttag cggtcgccat gaagactttc acgccttctt caaactcggt
     5341 caccggcagg atcgcggtga tacgttcgtc ctgttccagc ggtagcaggt tgacgatcgg
     5401 acgaccgcgc gcgccacgag tggcttccgg caactggtaa actttcatcg aatagacgcg
     5461 accacggctg gagaagcaca gaatatggtc gtgcgtgttc gccaccagca gtcggtcgat
     5521 aaagtcttct tctttaatac gtgcggcaga tttacctttc ccgccacgac gctgcgcttc
     5581 gtattcagaa agcggctgat atttaacgta gccctggtga gagagcgtca cgaccacatc
     5641 ttcctgggtg atcagatctt ccaggttgat gtctgcgctg ttggcggtga tttcagtacg
     5701 acgtttgtca ccgaactgtt cacgaaccag ctccagctct tcgcggatca cttccatcag
     5761 acgatcggcg ctaccaagaa tacgcaacag ttccgcgatc tgatccagca gctctttgta
     5821 ttcgtcgagc agtttttcgt gctcaaggcc ggtcagtttc tgcaaacgca gatccagaat
     5881 cgcctgagct tgctgttcgg tcaggtagta cagaccatca cgcacgccga actctggctc
     5941 cagccattcc ggacgcgcag catcgtcgcc agcacgttcc aacatggcgg caacgttgcc
     6001 cagctgccac ggattagcaa ccagcgcagt tttcgcttct gcaggcgtcg gcgcatgacg
     6061 gatcagttcg atgatcgggt caatgttcgc cagcgccacg gctaatgctt caaggatatg
     6121 agcacgatcg cgagctttac gcagttcgaa aatagtacga cgggtcacca cttcacggcg
     6181 gtgacgaaca aacgccgcga tgatgtcttt caggttcatg atcttcggct gaccatggtg
     6241 caatgccacc atgttgatac cgaaagaaac ctgcaactgg gtctgggagt agaggttgtt
     6301 gagcacaact tcaccgaccg catcgcgttt cacttcaatc acgatgcgca taccgtcttt
     6361 gtcagactcg tcacgcagcg cgctgatgcc ttccacgcgt ttttctttta ccagttccgc
     6421 aatcttctcg atcaggcgcg ctttgtttac ctgatacgga atttcgtgga cgataatggt
     6481 ttcacgtccg gttttggcgt caacttccac ttcagcacgg gcgcggatat ataccttgcc
     6541 gcgaccggta cggtaagctt cttcaatacc gcgacgaccg ttaatgattg ccgccgtcgg
     6601 gaagtccggc cccgggatgt gttccatcag cccttcaatg ctgatgtctt catcatcgat
     6661 atacgccaga caaccgttga tgacttccgt caggttgtgc ggcgggatgt tggttgccat
     6721 acctacggcg ataccggaag aaccgttcac cagcaggtta ggaattttgg ttggcatgac
     6781 gtcgggaatt ttttccgtgc cgtcatagtt atcaacgaaa tcgaccgtct ctttttcgag
     6841 atcggccatc agttcatggg caattttcgc cagacggatt tccgtataac gcattgccgc
     6901 cgcagagtcg ccgtcgatgg aaccgaagtt accctgaccg tctaccagca tgtaacgcag
     6961 cgagaatggc tgcgccatac ggacgatcgt gtcataaacc gccgcgtcac catggggatg
     7021 gtatttaccg attacgtcac caacgacacg ggcagatttt ttataggctt tgttccagtc
     7081 attgcctagt acgttcatgg cgtaaagtac gcgacggtgt accggcttca ggccatctcg
     7141 gacatctggc agcgcacggc caacaatgac cgacatcgca taatccagat aggagctctt
     7201 tagctcttcc tcaatgttga ccggtgtaat ttctctcgca aggtcgctca tctaaccgct
     7261 atccctctac tgtatcccgg attcaaaggt cgcaaattat aacacagccg cgcagtttga
     7321 ggtaaaccta tacgctttat tcacatccaa tgcctgatat actcgtttgt cttgccaatt
     7381 acggagtaga agtgccaatg aatgccgaaa aatcgccgga aaaccataac gtagaccacg
     7441 aagagatcgc taaatttgaa gccgtcgcct cccgctggtg ggatcttgaa ggtgagttca
     7501 aaccgttgca ccgcattaac ccgctgcgtc tgggctatat tgccgagcgt gctggcggtt
     7561 tatttggcaa aaaggtgctc gatgtcggtt gtggcggcgg cattctggcc gagagtatgg
     7621 cgcgcgaagg cgcgacggtg accggtctgg atatgggctt tgagccattg caggtggcaa
     7681 agctgcacgc gctggaaagc ggcattcagg tggattacgt gcaggaaacc gtggaagagc
     7741 acgcggcaaa acatgccggg cagtatgatg tggtgacctg catggagatg ctggagcacg
     7801 tacccgatcc gcagtcagtg gtcagagcct gtgcgcaact ggtgaaacca ggcggcgatg
     7861 tctttttctc gacgcttaac cgcaacggca agtcatggct gatggcggtg gttggcgcgg
     7921 aatatatttt gcgcatggtg cccaaaggta cgcatgatgt gaagaagttt attaaaccgg
     7981 cagagctgct tggctgggtg gatcagacca gtttaaaaga gcgacatata accgggctac
     8041 attacaaccc gatcactaat tcctttaagc tcggccccgg cgtggatgtg aactatatgc
     8101 tgcacacgca gaataagtga ggttgatgtt tggccgcgcc aatgcctgat gcgacgcttg
     8161 cagcgtctta tcaggcctac aaatgctctc cgtaggccag ataaggcgtt tacgccgcat
     8221 ccggcaattg tgatggtcag acagcgatta ccatttcacc gtcatcgaca aaaaccctgc
     8281 cgtctgggca aaatcatcac tccctttctg ccacgccacg ctgccgcgca gggacactcg
     8341 ctgactgata ttgcccgtga ctcccacttt tatttcaccc cgtagcttca ccgcatcgtc
     8401 actgatagtg ctgccatctt cttcaatttc cgtcgcatgg ggatcgtgat agtaattcag
     8461 atctaatgtt ggtgtgacac caaccgcggt acgccattcg ctgtgtaaac ccagccgcgt
     8521 ctgaatatca tccccctgcg attgtgacac gcgcgcatgg ttagcggcgg taaaatcatc
     8581 ctgctgcacg ccctgataaa tcacctgcgc ctgcggttca atcaccacac cacgccccgg
     8641 taaccactga tacccagctt ccagcgaggc gataatcccc gacgaatggt aacgatccac
     8701 gccatcttca tgttcggaaa catcattgtt aaaccacgcg tattgcagcc agctatccag
     8761 ccaggccccc tgcttctgat taccgtgctg ataccagctt gaggtcagcc caacggcata
     8821 accgtggttc tggttatcgg cgcgagttcc ggtcatattc gagcggctgt cgccctgatt
     8881 atcactgtag ccaccaaccg ccccaagcat ccactcgcca tccgtgcccc agcgcccgct
     8941 aaacaagccg ccgctaagct gcaccgtaga agtgtcttcc tgctgagcca gttgccctgc
     9001 cgccgtgtaa tgataacgtc cgccgataac acgtaaattc agcgtctgac catcgccacc
     9061 agcgtgatcg cgtcgctcca tcataaacgc ctgatttgcc gcccgcaggt tattgagata
     9121 accgccaact ttggcattca gcaccggctg gtaagcaggc gtaggggccg ggtcgggggt
     9181 tgggtcagga tccggcgtgg gatcaggatc gggagtcggg tctggctcag gtggcgatgg
     9241 cggcgttact tcttgcgatc gcagatacca gtcgttgtta tcttccacta gcgtgtagtc
     9301 atacgctccc atgttgacgt agccactgcc agccagactg aactgtgcat tgttttgaaa
     9361 ctgcgtggga tcagctgcga aatcaaccac tttaatgcct gtcgatgtcg gctcaccaat
     9421 ccctgtaatg gagttaacca ccacagttgt gttgccagca gtattaccgt tcatcaccaa
     9481 ttgatcgctt accgagtcat cgccgtttaa ttcgctgtcg aatagtagcg taccgccgcc
     9541 ggtataatca ccgtttaccg tcagcgtatc gccagcgacg ccattttgca ggctaaccgc
     9601 accggacgta ttggtcagat caccattaac cgtcacgtca gcaagatagg tcaggctgtc
     9661 agtttcaagg ttggtattaa gctcgcctgt caacgtcacg ccatcggtag catttaccag
     9721 cgtcccatcg ccgttaaata gtgaggcatt aagggcgacg gaagtatcct gcccggtcag
     9781 acgcaaaacc gtaccatcgc tgatgtcgat agtgccgctg gaagtagcat caattgactg
     9841 aatatcctga tccgcgccag taacgaacgt tgccccatca ccaacccaca gtgacgaagc
     9901 ataaggaaag atatccgcaa catcgccttt cagggtgcct tcttcgacac gtaccgccga
     9961 ctgcgttgta ccgctggcgg tcagctccag cgtacccgcc cccgttttag taaatgtgct
    10021 accctcatcc tgatgctgcc cgctgctgtc agccatcagt gcgccccact gcgtgtctac
    10081 ccccgcatca actgccactt caccgtcggc gcgcatttca atatcacgcc catgctcagt
    10141 cgtcgtagag cgagtatcga cattggcagt aatctccatc acgctttctt gttacttatc
    10201 ggtaaagata acctggcggt tatagtgggt atctccaagc tgcgagttgt cgctcaccat
    10261 cagcgtaccg gaggcgattt gtgttgtgcc caaataacta ttattgtttg ccagacttac
    10321 ctgcccgtcg ctcgcatcga ccaccacgta gacgccatct ttcccgtcgc cgccgatatg
    10381 caaattgtcg ccacctgcaa gatctaccac atcctgactg ccgataaccg ttccgctgct
    10441 gccactgact tcaaggccat cattgtatga tgtgccgctc tgccaagtgg agaaatcaga
    10501 gagatcgagc acaccgccgt ttaacacgat cgactgcgga tcgtcctgga gagcggtaag
    10561 atctgccgcg tcgccttcca gcgaaagcac cgcaccatca tcaacgacta tatcgccggt
    10621 tagcgccatc gactgcgccc ctgccagcac gtagctgccg ttttgcgcaa tggtgagctg
    10681 accagcacct tcgatgatgc cagcaaaact gccctgatta acagtaacgt tgccaccagc
    10741 atcgatattt aaagtgccat tctgaaagcc cgtcaatgcg tgcacaaaag tttgttgggt
    10801 cgagccaacg tttagctcag cctgattctg atactgatca atactcccta tcgtcagacc
    10861 gtagcagtct tgcggatcgt cctggcaatg cgtatcgccg acattcatca gggagttgct
    10921 gcgacccagg gtaacttcac cgttttcaat ctgcatctca ccagtaaagt cattgttatc
    10981 tgcattaagt accagatcgc cggaacctgt tttggtgatt aacccggtac cagcaataga
    11041 gtcaacagct ccgtcattct ctgtattgcc gataaccagc gtttttccgt cggcaatatc
    11101 aaaggtaact tcacttaagc cgagatacat aaagccaccc gccgcagtgg agggaccatc
    11161 tccatagcct gctgcgctat tgttctcatc gactaacacg ccgccgttct ggctgtagct
    11221 gtcatcaaca gaaatatcaa taagataggg agccgtcgcg ctattggtat atatcgcccc
    11281 gccataacct tcggctgtgt tatttgtaaa ggcggtgtta tttattatcg tataacctga
    11341 aggatgcttg ctgtcgctat tattatcggt aacatcgatt gccccgccat cgccatcact
    11401 gtaacttgtt gatgtatatg cctggttatt gttaaaaaca tcatcactta aatagacgtc
    11461 attattaatg gtataaattg cgccaccttt gccatcatta gcgatattat tgatggcgtt
    11521 agtgacacgt aaatcgacgg caccagtatc attagtacca gaagaataga ttgcgccacc
    11581 atagccgcct gcgacgttac cggaaaaaat aacatcagta agattcagcg ttgagttttc
    11641 tttagcaaat atggccccgc cgttattata ttctcctgta acggtgttat tggcaaacag
    11701 agtcattcca gtttcatttt ctggtaatag tgaaaattcc gctccttgtt gcaaaaatac
    11761 ggccccaccg ctggcattat tggtcatatc cgaaaaaacc agccattgcc catcgg
//
LOCUS       DAJTRW010000109        11704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRW010000109 DAJTRW010000000
VERSION     DAJTRW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            116..985
                     /locus_tag="OO326_003327"
     CDS             116..985
                     /locus_tag="OO326_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5967603.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1188..1541)
                     /locus_tag="OO326_003328"
     CDS             complement(1188..1541)
                     /locus_tag="OO326_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5967604.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO326_003329"
     CDS             complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO326_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5967605.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3369..3662)
                     /locus_tag="OO326_003330"
     CDS             complement(3369..3662)
                     /locus_tag="OO326_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5967606.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3938..5194
                     /gene="yjeH"
                     /locus_tag="OO326_003331"
     CDS             3938..5194
                     /gene="yjeH"
                     /locus_tag="OO326_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5967607.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVSLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO326_003332"
     CDS             complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO326_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5967608.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6023..7459
                     /gene="aspA"
                     /locus_tag="OO326_003333"
     CDS             6023..7459
                     /gene="aspA"
                     /locus_tag="OO326_003333"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5967609.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7577..8878
                     /gene="dcuA"
                     /locus_tag="OO326_003334"
     CDS             7577..8878
                     /gene="dcuA"
                     /locus_tag="OO326_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5967610.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8994..9332
                     /gene="cutA"
                     /locus_tag="OO326_003335"
     CDS             8994..9332
                     /gene="cutA"
                     /locus_tag="OO326_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5967611.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9308..11005
                     /gene="dsbD"
                     /locus_tag="OO326_003336"
     CDS             9308..11005
                     /gene="dsbD"
                     /locus_tag="OO326_003336"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5967612.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11042..11617
                     /locus_tag="OO326_003337"
     CDS             11042..11617
                     /locus_tag="OO326_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5967613.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2814 a         3023 c         2879 g         2988 t
ORIGIN      
        1 atagagtgag cacggctatt tatgttattt taacaactat tactaccaag cccatttatc
       61 tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa cctacatggc
      121 cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact
      181 ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga
      241 actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata aactggatgc
      301 tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa tggcagaaaa
      361 catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac
      421 gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg
      481 tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc gagcaatcat
      541 tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct cactgttaga
      601 tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat atgacaccta
      661 ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc tttaccgctt
      721 taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac
      781 gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa
      841 aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca
      901 accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac aatccaccag
      961 caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca
     1021 gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc
     1081 cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg
     1141 aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca gttcgggcac
     1201 ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc
     1261 gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga
     1321 tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg
     1381 ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca
     1441 atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt
     1501 agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat
     1561 gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga tgaccaaaag
     1621 aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg cagggcaatt
     1681 acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg
     1741 catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg ccagccacag
     1801 aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga
     1861 tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa
     1921 cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag
     1981 cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca
     2041 ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt
     2101 cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt ttctctttca
     2161 tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta
     2221 cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga
     2281 tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg
     2341 tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca
     2401 gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca
     2461 gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc
     2521 cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct tcagcgatga
     2581 tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt
     2641 tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt
     2701 ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca
     2761 cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga
     2821 ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg
     2881 taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct ttcagttctt
     2941 caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg
     3001 cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg
     3061 taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca
     3121 tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga
     3181 tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga ccgagggtaa
     3241 ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac
     3301 cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg
     3361 tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct
     3421 tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca
     3481 actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc
     3541 agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca
     3601 gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc
     3661 attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg
     3721 gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa
     3781 gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat
     3841 cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc
     3901 agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact
     3961 ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc
     4021 cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt ggcccgtttt
     4081 gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag
     4141 cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac
     4201 cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac aaattgccgc
     4261 cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg
     4321 tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg ctaatctaca
     4381 aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat
     4441 caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc
     4501 tgcgttatca gtgatgttct ggtgttttgt cagtctggaa gcatttgccc atcttgcctc
     4561 ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc tgctgctggc
     4621 aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca
     4681 aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt
     4741 atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca tttatataca
     4801 gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg
     4861 cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt
     4921 gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta tttatgccaa
     4981 tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg
     5041 tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt
     5101 cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt ttctgccaaa
     5161 acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc
     5221 gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac tcaccatcaa
     5281 aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca
     5341 aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac
     5401 cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata atcagcgaaa
     5461 cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc tgcatcagca
     5521 cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa
     5581 ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct
     5641 caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta
     5701 atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca gcttatattg
     5761 tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg
     5821 gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga
     5881 ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa
     5941 tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg
     6001 cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg
     6061 taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa
     6121 cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat
     6181 ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc
     6241 gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt
     6301 cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt
     6361 gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa
     6421 cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg
     6481 tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg
     6541 ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca
     6601 ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga
     6661 agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac
     6721 agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact
     6781 ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga
     6841 ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa
     6901 aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa
     6961 cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc
     7021 ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc
     7081 agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc aggccatgtt
     7141 cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg
     7201 catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac
     7261 ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga
     7321 aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact
     7381 tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac
     7441 tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac
     7501 gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa
     7561 aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg cgatcttctt
     7621 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
     7681 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
     7741 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
     7801 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
     7861 ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
     7921 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta ctgcagtggt
     7981 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
     8041 cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
     8101 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
     8161 tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc gcggtgaaaa
     8221 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
     8281 tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
     8341 gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt
     8401 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
     8461 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
     8521 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
     8581 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
     8641 gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt
     8701 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
     8761 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
     8821 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
     8881 atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt
     8941 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
     9001 atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg gatgaagcga
     9061 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
     9121 tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
     9181 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
     9241 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
     9301 acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca cttccgtttt
     9361 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc
     9421 ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta
     9481 ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgagca
     9541 gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
     9601 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
     9661 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
     9721 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca
     9781 gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
     9841 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa
     9901 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
     9961 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
    10021 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
    10081 aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat
    10141 gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg cgattgccgg
    10201 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca
    10261 aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct
    10321 gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat
    10381 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
    10441 gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt
    10501 tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat
    10561 aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
    10621 gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa cggtagatga
    10681 gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga
    10741 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
    10801 agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt
    10861 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
    10921 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
    10981 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
    11041 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
    11101 caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg
    11161 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
    11221 agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
    11281 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt
    11341 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
    11401 gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg aagttgacga
    11461 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
    11521 ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg
    11581 cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat
    11641 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag
    11701 atta
//
LOCUS       DAJTRW010000110        11646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRW010000110 DAJTRW010000000
VERSION     DAJTRW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            598..795
                     /locus_tag="OO326_003338"
     CDS             598..795
                     /locus_tag="OO326_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967614.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(745..1938)
                     /locus_tag="OO326_003339"
     CDS             complement(745..1938)
                     /locus_tag="OO326_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967615.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2074..3798
                     /gene="iucA"
                     /locus_tag="OO326_003340"
     CDS             2074..3798
                     /gene="iucA"
                     /locus_tag="OO326_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5967616.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3799..4746
                     /gene="iucB"
                     /locus_tag="OO326_003341"
     CDS             3799..4746
                     /gene="iucB"
                     /locus_tag="OO326_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5967617.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4746..6488
                     /gene="iucC"
                     /locus_tag="OO326_003342"
     CDS             4746..6488
                     /gene="iucC"
                     /locus_tag="OO326_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5967618.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6485..7822
                     /gene="iucD"
                     /locus_tag="OO326_003343"
     CDS             6485..7822
                     /gene="iucD"
                     /locus_tag="OO326_003343"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5967619.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7828..10023
                     /gene="iutA"
                     /locus_tag="OO326_003344"
     CDS             7828..10023
                     /gene="iutA"
                     /locus_tag="OO326_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5967620.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10574..10717)
                     /locus_tag="OO326_003345"
     CDS             complement(10574..10717)
                     /locus_tag="OO326_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967621.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10956..>11646
                     /locus_tag="OO326_003346"
     CDS             10956..>11646
                     /locus_tag="OO326_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5967622.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEV"
BASE COUNT         2953 a         2894 c         2989 g         2810 t
ORIGIN      
        1 tggagtgaaa caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa
       61 atcatgactc ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta
      121 gttttacagg atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt
      181 cccaaaaaac ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag
      241 tcaggtgttt gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc
      301 agtaaaaata cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa
      361 aaagaaacct ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc
      421 cctgataccc atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa
      481 cgcgcagcgc agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac
      541 agagcaacgc tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg
      601 gagattacac cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc
      661 agaacactgc ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtagcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata acatttattg ttgataatga gaatcattat tgacataatt
     2041 gttattattt tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc
     2101 acagatgtgg ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa
     2161 ctgacagaat acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat
     2221 ttcagagtgg cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg
     2281 gtcacggcat caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat
     2341 aagctacgcc atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg
     2401 ctggaaagcc atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg
     2461 cgtgaaaaag cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc
     2521 caccctgcgc ctaagtctca tgaaccgttt aaccggcggg aggctgaacg ctacctgcct
     2581 gatatggcac cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt
     2641 gaaagtttgc atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct
     2701 cagttactca acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg
     2761 gaatatcttt tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac
     2821 ttaggtgagg ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc
     2881 accagccgcg atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact
     2941 ctgtccgtga aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc
     3001 tggcagatgc tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc
     3061 gggctgctcg atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat
     3121 ctgctggtgg atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca
     3181 cctgatggcg gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt
     3241 atcactgtgc aacaggccgc ccatgcatgg gttgatgcat actgtcagca agtgctaaag
     3301 ccgctgttta cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt
     3361 cttgtccaga tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc
     3421 gcttttatgc ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat
     3481 attttcaccc atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact
     3541 tttgctgtga ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct
     3601 cgtgtacgag catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac
     3661 tacgtgctgg aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat
     3721 cataacgaga acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg
     3781 caggctcagg aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc
     3841 tgtgaaaaac tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg
     3901 cactgtcccg gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc
     3961 gccgcaccac aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag
     4021 gcgctgacgc tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag
     4081 ttaccgttat ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca
     4141 gagcgtgaga tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt
     4201 tacgatccac gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca
     4261 gaacgtttca cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc
     4321 tcactggagg tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg
     4381 ctgattgggt gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa
     4441 gaccgcattg gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt
     4501 ggtgaacagc aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat
     4561 tacctgctgc tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac
     4621 cagcgcctgt tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc
     4681 ccacataagc gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt
     4741 ctgtaatgaa tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt
     4801 ctgagatgga gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca
     4861 ttaacctgcc gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt
     4921 ggattgatgc ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga
     4981 tgcagctaaa gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt
     5041 tgtatgccac gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca
     5101 gtgacctgat taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat
     5161 tcgtttttaa taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag
     5221 agtataccaa caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc
     5281 gctgcgataa cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt
     5341 ttacacgctt cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctgccgctgc
     5401 cggtacatcc gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgccg
     5461 aaggcaggat ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc
     5521 gtaccctgac caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct
     5581 acaatacctc atgctaccgg gggatccctg gcagatatat cgctgccgga ccactggctt
     5641 cacgctggct gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga
     5701 tccttggtga accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg
     5761 ctccctatcg ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc
     5821 tgaaaccgga tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc
     5881 agccgctggc aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc
     5941 aattgttccg ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc
     6001 ttattgcaca cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc
     6061 tgctgaaaga cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact
     6121 ctttgcctca ggaggtgcgt gatgtcacat cccgtctgag tgcggactac ttaatccatg
     6181 atttgcagac gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc
     6241 ttggcgtacc tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga
     6301 acaaacatcc acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca
     6361 ttcgcgtggt gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca
     6421 tgctgccgaa ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg
     6481 aatcatgaaa aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat
     6541 tgctgcattg tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca
     6601 tttttcctgg catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa
     6661 agatctggtc agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa
     6721 gcacaaaaag ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt
     6781 ttctgactat ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt
     6841 tgaaaatatt gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga
     6901 atattttgcc cacaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt
     6961 gaagcatgtg acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct
     7021 gagtggaaaa cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa
     7081 tgcattacgc ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa
     7141 ttttaacgca ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc
     7201 aggcttctcc ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac
     7261 atcggatggc atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt
     7321 tgaagttctg agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaactct
     7381 ggaaagtagt ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga
     7441 gagcctggaa agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat
     7501 acttccctca ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga
     7561 tgacttcact ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag
     7621 catgcaaact catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg
     7681 tattcttaat cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat
     7741 tcagtggcgc agcggcagcc ggaaaaaacc acagccggag gctgcttctt taactcacta
     7801 tacagcaaat attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt
     7861 ccgctgcttc ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc
     7921 atagtgtctg ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt
     7981 atcgaaaatg ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg
     8041 gctcagttaa tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac
     8101 atgcgtggcc gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc
     8161 gacagccgac aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc
     8221 ggcgcgacgg ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa
     8281 aaaggtcagc cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc
     8341 agtaaagatc acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc
     8401 ggacgtcttt ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc
     8461 accctgcttg ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga
     8521 accggtacgc tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa
     8581 agtcagggag acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg
     8641 agcagcacac catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg
     8701 catttgatca gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag
     8761 gtttactacc gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa
     8821 caggcgacgg ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact
     8881 ctgaacagcc aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag
     8941 cgctttacct ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg
     9001 aacaaccata agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg
     9061 gccttcctgc aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc
     9121 tatcagtata ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt
     9181 gctaccggga aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac
     9241 tttctgttca atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat
     9301 ttttcccagg gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt
     9361 gcagcagtga acggccatct tcccctgaca aagagcgtga acgtcagcga cagtaagctg
     9421 gaaggcgtga aagtcgattc ttatgaactg ggctggcgct ttatcggtga caacctgcgg
     9481 actcaaatcg cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg
     9541 accatcagtg tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac
     9601 ctgatcccgg atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg
     9661 aaagtgaacg gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca
     9721 gcttacatta actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc
     9781 gacgtaagcg atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt
     9841 agttggcagc ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac
     9901 tataccactg tctggggaca gcgtgcacct ctgtactaca gcccgggtta cggccctgct
     9961 tcactgtacg actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc
    10021 tgacggggat gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg
    10081 ttgtttacag ccagtataat atacagcctt aacaagatat tccgtctccg gaatgggctg
    10141 ttcaccactg aataacatta tgtctgtttt atggatcttt gactgaaatt tatcgcatat
    10201 cctcatttca ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc
    10261 tggcagatcc tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt
    10321 gctgcaatga aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta
    10381 aaaagaggta cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc
    10441 tgtcataaag tccggtaatg gagattgtta atacgatggt gcacgatgca tcgtcagtgc
    10501 ccggcccgga ggatgccccg tagctacata cggatcaggg ttggcaatat cgaatggcag
    10561 gctataggca aagttaaagc cccatggagt agcacaaaat atgccgcaca aaggaaacgg
    10621 tctgaataac gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga
    10681 tcgctaattc gtcgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc
    10741 gaaatattaa acattacccc tccccacatt gggcaaaaaa aaacaaaaaa cgatcaatta
    10801 gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt tacaatatag
    10861 aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt aattcttaat
    10921 actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac tcccttaaat
    10981 atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga gtttctggta
    11041 aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt acagtaaatg
    11101 cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt gcacaaaata
    11161 aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac ggagataaat
    11221 tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt ggcgcagcta
    11281 ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac cctcaggccg
    11341 cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga aattccaatg
    11401 attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc accccggcag
    11461 agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc acttctgacg
    11521 gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt attaatggtg
    11581 aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct agtctatttg
    11641 aggtca
//
LOCUS       DAJTRW010000111        11466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRW010000111 DAJTRW010000000
VERSION     DAJTRW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(247..522)
                     /gene="yjfN"
                     /locus_tag="OO326_003347"
     CDS             complement(247..522)
                     /gene="yjfN"
                     /locus_tag="OO326_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5967623.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(671..1000)
                     /gene="bsmA"
                     /locus_tag="OO326_003348"
     CDS             complement(671..1000)
                     /gene="bsmA"
                     /locus_tag="OO326_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5967624.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1182..1931
                     /gene="yjfP"
                     /locus_tag="OO326_003349"
     CDS             1182..1931
                     /gene="yjfP"
                     /locus_tag="OO326_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5967625.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(1928..2683)
                     /gene="ulaR"
                     /locus_tag="OO326_003350"
     CDS             complement(1928..2683)
                     /gene="ulaR"
                     /locus_tag="OO326_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5967626.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2791..3855)
                     /gene="ulaG"
                     /locus_tag="OO326_003351"
     CDS             complement(2791..3855)
                     /gene="ulaG"
                     /locus_tag="OO326_003351"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5967627.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4210..5607
                     /gene="ulaA"
                     /locus_tag="OO326_003352"
     CDS             4210..5607
                     /gene="ulaA"
                     /locus_tag="OO326_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5967628.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5623..5928
                     /gene="ulaB"
                     /locus_tag="OO326_003353"
     CDS             5623..5928
                     /gene="ulaB"
                     /locus_tag="OO326_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5967629.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            5938..6402
                     /gene="ulaC"
                     /locus_tag="OO326_003354"
     CDS             5938..6402
                     /gene="ulaC"
                     /locus_tag="OO326_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5967630.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6416..7066
                     /gene="ulaD"
                     /locus_tag="OO326_003355"
     CDS             6416..7066
                     /gene="ulaD"
                     /locus_tag="OO326_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5967631.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7076..7930
                     /gene="ulaE"
                     /locus_tag="OO326_003356"
     CDS             7076..7930
                     /gene="ulaE"
                     /locus_tag="OO326_003356"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5967632.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            7930..8616
                     /gene="ulaF"
                     /locus_tag="OO326_003357"
     CDS             7930..8616
                     /gene="ulaF"
                     /locus_tag="OO326_003357"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5967633.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8745..9020)
                     /locus_tag="OO326_003358"
     CDS             complement(8745..9020)
                     /locus_tag="OO326_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5967634.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9347..9742
                     /gene="rpsF"
                     /locus_tag="OO326_003359"
     CDS             9347..9742
                     /gene="rpsF"
                     /locus_tag="OO326_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5967635.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9749..10063
                     /gene="priB"
                     /locus_tag="OO326_003360"
     CDS             9749..10063
                     /gene="priB"
                     /locus_tag="OO326_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5967636.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10068..10295
                     /gene="rpsR"
                     /locus_tag="OO326_003361"
     CDS             10068..10295
                     /gene="rpsR"
                     /locus_tag="OO326_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5967637.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10337..10786
                     /gene="rplI"
                     /locus_tag="OO326_003362"
     CDS             10337..10786
                     /gene="rplI"
                     /locus_tag="OO326_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5967638.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(10857..11420)
                     /locus_tag="OO326_003363"
                     /pseudo
     CDS             complement(10857..11420)
                     /locus_tag="OO326_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2719 a         2827 c         3020 g         2900 t
ORIGIN      
        1 gaaatatgct tctccgccaa tggcgcaggc gtggtgtcag gtgatgttag atacgcgcgg
       61 cggcgtacgg ttgtcagagc agatccagaa tgatttattg ctgcgggcga cggggggagt
      121 gtgtgtgtaa gcgtatacga ctgatgcgac gctggtttcg attaactaaa tgaaatatgt
      181 gaaaattgta ggccggacaa ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga
      241 tgacactcat gcatacagta tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta
      301 catggtaata atgcggtacc atgacgcacc ttgttcatcg gcctttaacg ccacgatacg
      361 ttccacgtcc tgcggatccc ctgaaatatt gctaactgag atctgaccta tttcattcag
      421 gcccgtcacg cagtcagcac tggcgaattc tgcagactgt gccgtggcgc tcgcaggata
      481 agtcactaat tgcagcgagg gtgaggcaag taattgtttc atcgtcagct ccatttttcg
      541 tttccccaca tgttgcaggg cattccatcg cggtaataaa cgcccaagct tccagccggt
      601 aatttattgt gcacaggtaa acgcataggg acgcaaagca atgtaaagct gactatgtaa
      661 gttgtctgat ttatttacgg tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg
      721 tttcgtccac catgacgaca acgtaataat cagcttttgc agcgacagct tttgctctga
      781 tttctgctaa tgcgtcatcc ggagaacccc gaaccatcgt gcttacgcta cctattcgct
      841 gtaacccttg cgtctggtcg cggcgaatct cttgcggatg gtccgttact ggcggtgctg
      901 gctgtggcgt accttgcagt gcgctacagg cacttaacat caacaccaat aataaactgg
      961 caaaccggta aataacgcta ttacgtttcc tgctaaccat agtgtagtgc cttattaatt
     1021 taactttggg ggaatgttcc cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg
     1081 acgaaagcgt aacccacatc tcaacattat gaactaatac cgccagcaga aacttgtgct
     1141 aaggatttct tcatgtcata ccagacaaaa aggagagaca gatgattgaa atagaatcac
     1201 gcgagctggc agatattccc gttcttcatg cttatcctgt cgggcaaaaa gataccccgt
     1261 taccgtgcgt aattttttat cacggcttta cttcatccag cctggtgtat agctattttg
     1321 ccgttgcgct ggcgcaggct ggtttgcggg tgatcatgcc ggatgcgccc gatcacggta
     1381 gccgttttag tggcgacgaa gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa
     1441 gtatgcagga attcactact ttacgtgcgg caatagccga agaaaactgg ctgcttgatg
     1501 accgtctggc agtcggtggc gcgtcgatgg gcgcgatgac ggcactggga attaccgctc
     1561 gccaccccac ggtgaaatgt accgccagca tgatgggatc gggctatttt acatcgctcg
     1621 cccgttcact gtttccaccg ctgatacctg aaacggcagc acagcagaat gaattcaata
     1681 aaattgtcgc gccgctggca gagtgggaag cgacaaacca cctggaacaa cttggggaca
     1741 gacctctact gctgtggcat ggcctcgacg atgacgttgt gcctgccgac gaatcactac
     1801 gtttgcagca ggccttaagc gagacgggac gggataaact gctaacctgt tcatggcagc
     1861 caggcgtgcg tcaccgcatt acgcctgagg cgttagatgc tgccgtgaca tttttccgcc
     1921 agcatcttta aacacgcaga atgctgaccc cttgcgcttc cagttgttgc aggatttccg
     1981 ggttagcatt tttgccggtg atcagcatat cgatttgatc ggcacggcta aaaagcattc
     2041 ccgcgcgttc gccaatctta ctgctatcaa ccagtaccac cagtttccct accacgctca
     2101 gcatcttctg ctctgccatt gctgtcagca tatcggtttt atacaaccct tctgcggtca
     2161 gcccttttcc gctggtaaac atccagtgcc cggcatagag actgttttcg ctgccctgcg
     2221 ggcttaaagt gatggactga cttttgttgt actgtccacc cataatgatc acgctgtcat
     2281 gttcttgatc gatcaggtaa tttgccagcg gtagataatt agtgatgatt tgcactggct
     2341 tgccacacat ttcccgccca agcagaaacg cggtggagcc gcagttgatg actacgcttt
     2401 cgcccggatt aaccagctgc gaggccgctt tagcaatacg tactttttca tcgtgattct
     2461 gcgcctgatg cagattcatc ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc
     2521 cattgcgcac ctttttcagt ttgccgcttt cgccaagttt attgatatcg cgtcgcgcag
     2581 tggcaggcga aattcccaga cgctcaacga ctttctcaac ggtcacaaag cccaattgtg
     2641 cgagcatttc caggaggatt tgatgtcttt gtgcttcagt catgagctat tccgataaaa
     2701 tttgattttt ttagatgata tttgaaatag ccaggaaata ctacgccgga tagcgcgaac
     2761 ttctccacgc tatccggtat aaacgacaga ttacaggaat gacttgaacg gcagatccgg
     2821 ttcaatagtg aagcaatcat cgaaaccgcg cggatagtgg tactcgaagt tgtctttatc
     2881 cagcggccag gtaaatttgc cgccaacctg ccagataaac ggcttgaagc catacttcag
     2941 acgatctttt ttgatctccc acagcacgcg gatctcttgc ggatcggcct ggaagtttga
     3001 ccagatgtcg tggtggaacg ggataaccac tttcgcattc agggcttcac ccatacgcag
     3061 catatcggcg ctggtcattt tgtcggtaat accgcgcggg ttctcaccat acgatcctaa
     3121 cgccacatcg atctgatgtt cgttaccgtg cttcgcataa tagttagagt agtgggaatc
     3181 gccactgtga tatagggaac cgccaggggt tttgaacagg tagtttactg cgcgatcgtc
     3241 catgccatct ggcagtacgc cagccgcttt ttgatcggca ggcagggtga tcagtgcagt
     3301 acggtcgaaa gcgtcaagcg catgaatttc aatgtctttc acttttacaa catcgcccgg
     3361 tttgaccacg atgcaacgct ctttcggtac gccccagcca atccacaaat caacgcaggt
     3421 tttcggtcca atgaatggta cgtcatcagc acagttctgc atcacggcag cagcgacgtt
     3481 aacgtcgata tgatcgttgt gatcgtgagt cgccagtacc gcgtcgatct ggcgaatcgc
     3541 aaacggatca agaacaaacg gggtggtgcg taggtttggc tgcagttttt tcacgccagc
     3601 catgcgctgc atctggtgac cctgtttcat taacgggtta ccgtgacttt gtttgccagt
     3661 gccgcaccag aaatcaacgc aaacgttggt gccaccttct gatttcaacc agatcccggt
     3721 gcagcctagc caccacatcg caaatgtgcc aggggcgacc tgctcttgtt caatttcttc
     3781 attcagccag ctaccccact ccgggaaagt gctcaggatc caggattctc gggtgatact
     3841 tttcacttta ctcatcgcca tttaccttca tgatagttca attcgaatca acatgtgatt
     3901 ggttttgatt aatcctgaca ctattccttc aggaaggtaa tgaccatttt ttgacttttt
     3961 gccagggaag ttgttgttga ttttgagtgg ggaaagattt aatggaatgt gtagttaaat
     4021 taactaaatg aatttaaata aataattgtt tcgttgtgtg aatcccactc tatccatgtg
     4081 gaattatttg cgggtcgcgt cacatttaat cataaataat cttgttgtga ttacttttga
     4141 aaattagagt gagtgcacaa cattccgggt gtgcggaata cccggttacc tcttcttcag
     4201 gagatcgtta tggagatcct ctacaacatc tttaccgtgt tttttaacca ggtcatgacc
     4261 aatgccccgt tgttgctggg tattgtgacc tgtctgggct acatcctact gcgcaaaagt
     4321 gtcagcgtta ttattaaagg cacgattaaa accataattg gtttcatgtt gttgcaggca
     4381 gggtccggca tcctcaccag caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac
     4441 ggcattaacg gcgcaatttc cgatacttac gcgtcgatga tggcaaccat cgaccgcatg
     4501 ggcgatgcct atagctgggt aggttacgcg gtgttgttag cgctggcgct gaacatctgt
     4561 tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca tgctgactgg tcatatcatg
     4621 ttccagcagg cggggctgat tgccgttacg ttgtttattt tcggctactc catgtggaca
     4681 accattatct gcacggcgat tctggtttcg ctctactggg gcattacctc caacatgatg
     4741 tacaagccga ctcaggaagt gacggacggc tgcggtttct ccatcggtca ccagcaacag
     4801 tttgcatcat taattgccta taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt
     4861 gaagacctca aactgccagg ctggctgaat attttccacg acaacatcgt ctccacggcg
     4921 attgtcatga ccatcttctt tggtgccatt ctgctctcct tcggtatcga caccgtgcag
     4981 gcgatggcag gcaaagtgca ctggacggtc tatatcctgc aaaccggttt ttccttcgcg
     5041 gtggcgatct tcatcatcac tcagggtgtg cgcatgtttg tggcggaact ctctgaagcg
     5101 tttaacggta tctcccagcg cctgatccct ggtgcggttc tggcgattga ctgtgcggct
     5161 atttatagct tcgctccgaa cgccgtggtc tggggcttta tgtggggcac catcggtcag
     5221 ctgattgcgg ttggcatcct ggtcgcctgc ggttcctcga tcctgattat cccaggcttt
     5281 atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg ctaaccactt cggcggctgg
     5341 cgtgcggcgc tgaagatttg tctggtgatg gggatgatcg aaatctttgg ttgcgtctgg
     5401 gtggtgaaac ttaccggtat gagtgcctgg atggggatgg cggactggtc gattctggca
     5461 ccgccgatga tgcagggctt cttctccatc ggtatcgcct ttatggccgt catcattgta
     5521 attgcactgg cttatatgtt cttcgctggc cgcgcactgc gcgcagaaga agatgcagaa
     5581 aaacaattgg cagaacagtc tgcttaataa ggagttttga ttatgaccgt acgtattctg
     5641 gctgtgtgtg gcaacggaca aggcagttcc atgatcatga agatgaaagt ggaccagttt
     5701 ttaacccaat caaacattga ccacacggta aatagctgcg cggttggcga atacaaaagc
     5761 gagttgagcg gcgcggatat catcatcgct tctacgcaca ttgcgggcga aatcaccgtg
     5821 accggcaaca aatacgtggt tggcgtgcgc aacatgctct ctcctgccga ctttggcccg
     5881 aaactgctgg aagtgatcaa agcgcatttc ccgcaggatg tgaagtaagg acgcgccatg
     5941 aaattacatg attcgctggc ggagaataaa tccatccgcc tgcaggctga agctgagaca
     6001 tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg cggcggatgt tgtcgagccg
     6061 cgttactacc aggcgattct ggatgcggtt gaacagcacg gtccgtactt tgtgctcgca
     6121 ccaggcctgg caatgccgca cggtcgcccg gaagagggcg ttaagaaaac cggttttgcg
     6181 ctggtgacgc tgaaaaagcc gctggagttc aaccacgaag ataacgaccc ggtggatatc
     6241 ctcatcacca tggcggcggt cgatgccaac acgcaccagg aagtgggcat catgcagatc
     6301 gtcaacctgt ttgaagatga agaaaatttc gaccgtttac gtgcctgccg taccgagcag
     6361 gaagtactgg atctcattga ccgcaccaac gcggcagctt aagaaggaat tgaagatgtc
     6421 attaccgatg ttgcaagtcg cgctggacaa ccagactatg gatagcgcct acgaaaccac
     6481 tcgcctgatt gccgaagaag tcgacattat cgaagtgggt accattctgt gcgtgggcga
     6541 aggcgtgcgt gcggttcgcg atctgaaagc gctctacccg cacaaaatcg tgctggcaga
     6601 cgccaaaatt gccgatgcgg gcaaaattct ttctcgtatg tgcttcgaag ccaacgctga
     6661 ctgggtgacc gtgatttgtt gtgcggatat caacaccgcc aaaggtgcgc tggacgtagc
     6721 aaaagagttt aacggcgacg tgcagatcga actgaccggt tactggacct gggaacaggc
     6781 gcaacagtgg cgcgacgcag gtattcagca ggtggtttat caccgcagcc gtgacgcgca
     6841 ggccgctggc gtggcgtggg gtgaagccga tatcactgcg atcaaacgtc tttccgatat
     6901 gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa gatctgccgc tgttcaaagg
     6961 cattccaatt catgtgttta tcgccggtcg cagcatccgt gatgccgctt ctccggttga
     7021 agccgcgcgt cagttcaaac gttccatcgc cgaactgtgg ggctaaggag cggatatgtt
     7081 gtccaaacaa atcccgcttg gcatctatga aaaagcgctc cccgccgggg agtgctggct
     7141 ggaacgcctg cgactggcaa aaacgttggg cttcgatttt gtcgagatgt cggtagatga
     7201 aactaacgag cgcctgtcgc gccttgactg gagccgcgaa cagcgtctgg cgctggtgaa
     7261 tgctattgtt gaaaccggcg tgcgtgtgcc gtccatgtgc ctttctgccc atcgtcgttt
     7321 cccgttgggc agcgaagatg acgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc
     7381 catccagttc gcccaggacg ttggcattcg cgtgatccag ctggcgggct atgacgttta
     7441 ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt
     7501 tgagatggcg agccgcgcgc aggtgacgct ggcgatggag atcatggatt atccgttgat
     7561 gaactccatc agcaaggcgc tgggttacgc gcactatctc aacaacccgt ggttccagct
     7621 ctacccggat atcggcaacc tgtcggcgtg ggacaacgat gtgcagatgg agttgcaggc
     7681 cggaatcggg catatcgtcg cggtgcatgt gaaagacacc aaaccgggcg tcttcaaaaa
     7741 cgtgccattt ggcgaaggtg tggtggattt cgaacgttgc ttcgaaacgc tcaaacagag
     7801 tggctattgc gggccgtacc tgattgagat gtggagcgaa acggcggaag acccggcggc
     7861 agaagtggtg aaagcgtgtg attgggtgaa agcgcgcatg gccaaagcag gcatggtgga
     7921 ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag ccaacatgga tctgccgcgc
     7981 tatgggctgg tgacctttac ttggggcaac gtcagcgcta tcgaccgcga acgcgggctg
     8041 gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcagacga tatggtggtg
     8101 gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc catcttccga cactgcgacg
     8161 catctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac ccactccact
     8221 catgccaccg catgggcgca ggcggggctg gcgatcccgg cattaggcac cacgcacgct
     8281 gactacttct ttggtgatat cccgtgtacg cgtggattaa gcgaagaaga ggtgcagggt
     8341 gagtatgaac tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc cgagccgctg
     8401 catacgccgg gaattgtggt gtatcagcac ggaccgttcg cctgggggaa agatgcccac
     8461 gacgcggtgc ataacgcggt agtgatggaa gaagtggcga aaatggcgtg gattgcgcgc
     8521 agtattaatc cacaactcaa tcatatcgac agctacctga tgaataaaca cttcatgcgt
     8581 aagcacggtc ctaacgctta ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt
     8641 cctcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc cctgcgaagg
     8701 gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg ataaatatcc
     8761 gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac gtggtagtac
     8821 gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac gtgcttcgct ttcagtggcg
     8881 aaattatgat tgatataaat cacgcctaag ctttgcacat cgtccatatt tctggcctgg
     8941 tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac agccagaagg
     9001 gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg ctctcctctg
     9061 tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga ccatttctga
     9121 tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa cttcaaatca gcggtttaga
     9181 ggttatttgc gaaaggggag atttatttct gctccgccct tgagtttagc ggggtatgcc
     9241 agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct ccccgggatt
     9301 cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc gtcattacga
     9361 aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga tcgagcgcta
     9421 cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact ggggccgccg
     9481 tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga tgaacgttga
     9541 agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg atgccgttat
     9601 ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga tggttaaagc
     9661 gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg atgatgctga
     9721 agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt ccggcaccgt
     9781 gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc agttcgtgct
     9841 tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg caggcatggt gtcaaatgcc
     9901 cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg tcggcagtcg
     9961 cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga gcaaaatggt
    10021 tttgcatgcc gagcagattg aattgataga ttctggagac tagccatatg gcacgttatt
    10081 tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc gactataaag
    10141 atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca agccgtatca
    10201 ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc gctcgctacc
    10261 tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat taatacgact
    10321 ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc tgggtagcct
    10381 gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac cgcagggtaa
    10441 agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg ctgaactgga
    10501 agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa tcaatgcact
    10561 ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg gttccatcgg
    10621 tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta agagcgaagt
    10681 tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac gaagtgagct tccaggttca
    10741 cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg ttattcaacg
    10801 agacgtaaaa acgccgacca ttggtcggcg ttttgctttc tatttttcgt caggcattaa
    10861 tttcgcaagt agatccattc accattctct tgtgtccata agtcaggatg gtttgctggc
    10921 gttagatgca tacgatcact ccttatgtct cggtcctgcg gcactcgctc gaaacctaaa
    10981 cccgagtaga ttgcggctga atttgataag gaacgtagac caattttcaa atcatggggt
    11041 aaatcagaat acgcgtttag caatgcagca atgatgaatc cgagggcatt agaaacgcct
    11101 gtaacagtgt gaagttggtc gatataaaga tcgattgttg gtagttcaga tgagcagcaa
    11161 ttaggcattt gggcaggaag ggttacaacg tatcctatcg tttggccggt atagtctgga
    11221 gtactataac caggtcccaa aacaacctcg tgtattgtgt atttactgtt gggttcgaag
    11281 gattcgacat cttgcaataa atatttcagt tctgcggtta acctcgcacc cgcgtaagct
    11341 ttattgtatt tctgggcgtg aatattatct aatgtagctt catggttcca tccaaaagaa
    11401 ttatcaccat tcattgttac tgaatcgcca cgggtttaac agacacctca gagtcattta
    11461 agatgg
//
LOCUS       DAJTRW010000112        11424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRW010000112 DAJTRW010000000
VERSION     DAJTRW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(217..292)
                     /locus_tag="OO326_003364"
     tRNA            complement(217..292)
                     /locus_tag="OO326_003364"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="OO326_003365"
     CDS             851..3103
                     /locus_tag="OO326_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967639.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="OO326_003366"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="OO326_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5967640.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="OO326_003367"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="OO326_003367"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5967641.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="OO326_003368"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="OO326_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="OO326_003369"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="OO326_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="OO326_003370"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="OO326_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5967642.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWASYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="OO326_003371"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="OO326_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5967643.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO326_003372"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO326_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5967644.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11279..>11424)
                     /locus_tag="OO326_003373"
     CDS             complement(11279..>11424)
                     /locus_tag="OO326_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5967645.1"
                     /translation="IVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQ
                     TRD"
BASE COUNT         2930 a         2811 c         2858 g         2825 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gagctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
    11281 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
    11341 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
    11401 gcagaatgat ggtgatgacg attc
//
LOCUS       DAJTRW010000113        11352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRW010000113 DAJTRW010000000
VERSION     DAJTRW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1046)
                     /locus_tag="OO326_003374"
     CDS             complement(<1..1046)
                     /locus_tag="OO326_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5967646.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1668
                     /locus_tag="OO326_003375"
     CDS             1537..1668
                     /locus_tag="OO326_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5967647.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1928..2923
                     /gene="pgl"
                     /locus_tag="OO326_003376"
     CDS             1928..2923
                     /gene="pgl"
                     /locus_tag="OO326_003376"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5967648.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2964..3917)
                     /locus_tag="OO326_003377"
                     /pseudo
     CDS             complement(2964..3917)
                     /locus_tag="OO326_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4101..5153
                     /locus_tag="OO326_003378"
     CDS             4101..5153
                     /locus_tag="OO326_003378"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5967649.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            5229..6662
                     /locus_tag="OO326_003379"
     CDS             5229..6662
                     /locus_tag="OO326_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5967650.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6845..9025
                     /locus_tag="OO326_003380"
     CDS             6845..9025
                     /locus_tag="OO326_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5967651.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(9166..10449)
                     /locus_tag="OO326_003381"
     CDS             complement(9166..10449)
                     /locus_tag="OO326_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5967652.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(10584..11276)
                     /locus_tag="OO326_003382"
     CDS             complement(10584..11276)
                     /locus_tag="OO326_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5967653.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2836 a         2553 c         2821 g         3142 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg tagcgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaatg
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tagtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tcaaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt ttatttgtgc cggaagtgag
     1621 ttgattgttg cgcgaatggg aagttggcca gaagttgtta tggcatagtg tttacggtcg
     1681 ttgatacagg taaacaaaag cggggagagt catactccct gaatagtcgc gaaaatatct
     1741 gtggcattgt caaattcgtg tcggtgacta atattttagt aatatgccat tcgaaatgga
     1801 gtgctaactt attgatttaa ttgatgtgcg atattgtatt ttatcgtgtt ttgttgtctg
     1861 ttttttatcc agtaagtgtt gttatatgta tgaaagataa gggatttatc atttttgagg
     1921 tgtgttcatg aagcaaacag tttatatcgc cagccctgag agccagcaaa ttcacgtctg
     1981 gaacctgaat catgaaggcg cactgacgct gacacaggtt gtcgatgtgc cggggcaggt
     2041 gcagccgatg gtggtcagcc cggacaaacg ttatctctat gttggtgttc gccctgagtt
     2101 tcgcgtcctg gcgtatcgta ttgccccgga cgatggcgca ctgacctttg ccgcagagtc
     2161 tgtgctgccg ggtagtccga cgcatatttc caccgatcac caggggcagt ttgtctttgt
     2221 aggttcttac aatgcgggta acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg
     2281 cgtcgtcgat gtagtcgagg ggctggacgg ttgccattcc gccaatatct caccggacaa
     2341 ccgtacgctg tgggttccgg cattaaagca ggatcgcatt tgcctgttta cggtcagcga
     2401 tgatggtcat ctggtggcgc aggaccctgc ggaagtgact accgttgaag gggccggccc
     2461 gcgtcatatg gtattccatc caaacgaaca atatgcgtat tgcgtcaatg agttaaacag
     2521 ctcagtggat gtctgggaac tgaaagatcc gcacggcaat atcgaatgtg tccagacgct
     2581 ggatatgatg ccggaaaact tctccgacac ccgttgggcg gctgatattc atatcacccc
     2641 ggatggtcgc catttatacg cctgcgaccg taccgccagt ctgattaccg ttttcagcgt
     2701 ttcggaagat ggcagcgtgt tgagtaaaga aggcttccag ccaacggaaa cccagccgcg
     2761 cggcttcaat gttgattaca gcggcaagta tctgattgcc gctgggcaaa aatctcacca
     2821 catctcggta tacgaaattg ttggcgagca ggggctactg catgaaaaag gccgctatgc
     2881 ggtcgggcag ggaccaatgt gggtggtggt taacgcacac taaccgctga tttaaccggc
     2941 gcagtctctc ctgcgccggt gtattaactt atctcctgta acgcatgtct ctggcgttcg
     3001 acgatattgg tccacaaatt gtctttatcg tcagtccaca aattaatcag caaggaaaaa
     3061 aagcgttcag ctgccggaga aagcacggca tctttgcggc gaataatccc caatgtccga
     3121 cgtataacgg gttccaccag tgggatacca ataatcgaag aatagggcgc atggggcatt
     3181 gccagaccag gaagcgccga aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa
     3241 agatgacgca cttcgtaaaa ccagtccagc ttccagggct tgtcggctag ctgttgttct
     3301 atcaacaatc ggttgccgct ggaggaacgt acgccaatca ttttgtaacc cactaactcc
     3361 tgccattcta caagctactt tttggccaat ggatggtcac gccgacaggc cagcacgaat
     3421 ggctcgttga ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt catgtttatg
     3481 ccaaagtctg attcgttaca cagcaccgat tccatgcaat tattcgtgcc ttgttccaga
     3541 atccgcactt taatattggg atacagctca ttaaatttac cgattgccag cggtaaaaaa
     3601 taaaatactg cggtcggaat acacgctaat gtcaccatac cacgatgata agcattcata
     3661 tcacgaatat taaaaagcgt ttcatcgaat ttctttatta attcccgcgc ctccgggagc
     3721 aacctttttc ctgcttttgt taacgtaacc ttgcgagttg tacgctcaaa tagttgaata
     3781 tgtaaatctt cctccatctt ttttattctt cgcgttaatg caggttgcgt aatattgagt
     3841 aatttagcgg cattgttaaa tgaactggac tctgccagta tgacaaatgc cttcatactt
     3901 gataattcat gcttcattat tactccggaa aatggaagcg acgattttgg ggggctggtc
     3961 gttaaaaatt ttaactgcat ttagccaact taaattaatg aaaaaatgtt attaatcgtt
     4021 gagctaaagt cattagagat gctttgtcct taatgtaacc atatcgcaat aagttatgtt
     4081 tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct
     4141 cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa
     4201 tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt ggcggcggta
     4261 atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt gctgatgtcg
     4321 attatctgtt tgctcaagtt atcgtccatg agcaacgtgt cgataccacg cctaactgcg
     4381 gcaatatgct gtctggcgta ggggcattcg ccattgaaaa tggtttgatt gcagcgactt
     4441 cgccagttac ccgtgtacgt atccgcaacg tcaatacggg tacgttcatc gaagctgatg
     4501 tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac ggcgtaccgg
     4561 gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa actggaaaag
     4621 ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc tgtatcgata
     4681 tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggt tatgaattac
     4741 cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc cggctacaag
     4801 cgggtaaagc aatgggctta ggtgatgtca gtaatatggt tatccctaaa cctgtgctta
     4861 tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg cattcttgcc
     4921 atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca ttggaaggca
     4981 ccgtcacccg acaaatcgtc ccttctgtag gatatggcaa tatcaatatt gaacacccca
     5041 gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat
     5101 ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt
     5161 cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca gaaagaacag
     5221 gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg atcccatgta
     5281 ttattggttt tatgccagct ccggcaggat taagcgaact ggcgtgggtg ctttttggta
     5341 tttacctggc ggccattgtg ggtctggtta tcaagccttt cccggaacct gtcgtactgt
     5401 taattgccgt tgctgcctca atggtggtgg tcggtaactt atccgacggt gcgtttaaga
     5461 ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc tcggcgttta
     5521 ccttaagcgc cgcatttgtg accaccggtt taggtaaact tattgcctat ctgctgattg
     5581 gtaaaatcgg taacaccatg ctgggtctgg gttacgttac ggtattcctc gatctggtac
     5641 tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca
     5701 acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt
     5761 acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc tttaccgcaa
     5821 tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg caaattagct
     5881 ggggtggatg ggcgctggcc gccggattgc cgggcatcat tatgctgctg gtcaccccgc
     5941 tggtaattta caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta
     6001 aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct
     6061 ttgtgctggc gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg
     6121 tggcaatcgt tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg
     6181 ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct
     6241 ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg
     6301 catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc
     6361 gttatttctt cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc
     6421 tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact
     6481 cctatggcgg catggttact cactatggcg gcgcggcagg tccggtcatc tttggcgtgg
     6541 gttacaacga tattaaatcc tggtggttgg tcggtgcggt actgacgata ttaaccttct
     6601 tggtgcatat caccctcggc gtgtggtggt ggaatatgct gatcggctgg aacatgctgt
     6661 aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca
     6721 cttacttatg taagctcctg gggattcact cacttgtcgc cttcctgcaa ctcgaattat
     6781 ttagagtata tccatttatt atctttctgc gcacttcacg gtgcgcagat atctggagca
     6841 tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc agtaataacg aaataattgc
     6901 cgaagaacat ttcaccggcg aaattaaaaa agaagaagca aaaaaaggca ctattgcctg
     6961 gtctattctc tcttctcata atacgtccgg aaatatggat aaacttaaaa ttaagtttga
     7021 ttcattagcc tctcacgata ttacctttgt tggtattgta cagaccgcta aagcgtccgg
     7081 aatggaacgt ttcccgctgc cgtatgtgct gaccaactgc cataactcac tctgcgccgt
     7141 cggcggcacc attaacggtg atgaccatgt ttttggttta tcggcagctc agcgttatgg
     7201 cggtattttt gtgcctccgc atattgcggt catccatcaa tatatgcgtg agatgatggc
     7261 tggcgggggc aaaatgatcc tcgggtcaga cagccacacc cgttacggtg cattagggac
     7321 aatggcagtc ggtgagggtg gcggtgagtt ggtaaaacag ctgcttaatg acacctggga
     7381 tatcgactat ccgggcgtgg ttgcggtgca tctgaccgga aaacctgcgc cgtatgtggg
     7441 gccacaggat gtggcgctgg ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa
     7501 caaagtcatg gagttcgttg gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag
     7561 cgttgacgtg atgaccacgg aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga
     7621 agtgcataac tggctggcgc tgcacggtcg cggtcaggat tactgccagc ttaaccctca
     7681 accgatggcg tactacgatg gctgcatcag cgttgattta agcgccatca aaccaatgat
     7741 tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac acactgaacc agaacctgac
     7801 cgacattctg cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta aagccaaact
     7861 ctcgctgctg gataaagtgg aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg
     7921 ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc
     7981 ctgtggcaat gacaccttct cgctggcggt ttacccgtca tcacagccgg tgtttatgga
     8041 tctcgccaaa aaaggtgtgg tagcagattt gattggcgca ggcgcaatca tcagaaccgc
     8101 attctgtggc ccatgctttg gcgcgggcga tacgccaatc aacaacggtt tgagtattcg
     8161 ccacaccacg cgtaacttcc cgaaccgcga aggctctaag ccagccaatg ggcagatgtc
     8221 agcggtggcg ttgatggacg ctcgttctat cgctgcgact gcggcaaacg gtggctattt
     8281 aacctctgcc agcgaacttg attgctggga caacgtgccg gagtacgcct tcgatgtaac
     8341 gccgtataaa aaccgtgttt atcagggctt tgtgaaaggg gcaactcagc aaccgctgat
     8401 ttacggaccg aacattaaag actggccgga attgggtgcg ctgactgaca atatcgtcct
     8461 gaaagtgtgc tcgaagatcc tcgacgaagt caccaccacc gacgaactga ttccttccgg
     8521 tgaaacctct tcttatcgtt caaatccgat tggtctggcg gggaatgtca gcgagctgac
     8581 agaggtgttt gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac
     8641 tgaaattggt agcatggtct atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc
     8701 ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt gccgaggagt acgcgactaa
     8761 acgctaccgt tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc
     8821 aacctttgaa gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc
     8881 gggtacgacg tttaaaggtt atgtgatcca tgaagatgcg ccggtaacgg aaattacgct
     8941 ttacatggaa agcctgacgg ctgaagagcg cgagattatc aaggcgggta gtttgattaa
     9001 cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact
     9061 tattgtcgga tgcgatgctg gcgcatctta tccgacctac aaatcgcatc gaaccgtagg
     9121 ccggataagg cgtttacgcc gcatccggca aatagttaat agctcttact tcttctcctc
     9181 tgcaaccact ttgctgccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt
     9241 gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa
     9301 cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat
     9361 ggcgctatca cggatcacca cctgaccgtt ggtattagca tcaacatcca gcgagcggcc
     9421 cagttgcgcc acgccatcac cggaagcatt gaaacggctg tttacggcga ggaaaccgta
     9481 atagatgtta gacagcgtag ccggtgcaaa cacatatgct tcttgctggg tacgtgagtt
     9541 caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac
     9601 atccccttca atatagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg
     9661 gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt
     9721 gttaatctgt acttggtcac cgtcagtacg cagtgctacc gccggatggt tacctgcatc
     9781 tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcagtc cattgttttg
     9841 tgaccagaag accgcagaac acagaacacc gatactgtcg ctgcgtttgc tctggcagct
     9901 atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg
     9961 acgccagtcg gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg
    10021 tttttcacct gtaccgtaca gagtgattcc acctggagca gcagggacat acaccgttcc
    10081 ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtgcgct tgataattgc
    10141 cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac
    10201 aacaaagtca ggttgcgcag gcagggttat cggggacgga ttccacgctg cagcacctgg
    10261 tgtcagggat gcaaaatagt gttgcgcatc gaaattcagc gcttcttttg ccgacagaat
    10321 cgggcgagaa gaggtaccag gcgcagtttg atcagaagga cgttgatcgg gtggtgttga
    10381 gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa
    10441 tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt
    10501 tttatactaa gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt
    10561 cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg
    10621 tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg ttgagcaaac
    10681 ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta
    10741 ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat
    10801 actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga
    10861 atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat
    10921 gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag atatccatct
    10981 acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt aacaacagcc
    11041 agttccattg caagtctgag ccaacatggt gatgattctg ctgcttgata aattttcagg
    11101 tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg
    11161 accgggtttg ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt
    11221 gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt gagcattgca
    11281 gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct
    11341 ttaattttgc tc
//
LOCUS       DAJTRW010000114        11232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRW010000114 DAJTRW010000000
VERSION     DAJTRW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO326_003383"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO326_003383"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5967654.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO326_003384"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO326_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5967655.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="OO326_003385"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="OO326_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5967656.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="OO326_003386"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="OO326_003386"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5967657.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="OO326_003387"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="OO326_003387"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5967658.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="OO326_003388"
     CDS             complement(5841..6881)
                     /locus_tag="OO326_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5967659.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="OO326_003389"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="OO326_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5967660.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="OO326_003390"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="OO326_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5967661.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="OO326_003391"
     CDS             7823..8263
                     /locus_tag="OO326_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5967662.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="OO326_003392"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="OO326_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5967663.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="OO326_003393"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="OO326_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5967664.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11232
                     /gene="rsxC"
                     /locus_tag="OO326_003394"
     CDS             9492..>11232
                     /gene="rsxC"
                     /locus_tag="OO326_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5967665.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEP"
BASE COUNT         2614 a         2891 c         2830 g         2897 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caacaggcta
    11221 atgcggaacc ag
//
LOCUS       DAJTRW010000115        11101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRW010000115 DAJTRW010000000
VERSION     DAJTRW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO326_003395"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO326_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5967666.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="OO326_003396"
     tRNA            complement(622..696)
                     /locus_tag="OO326_003396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="OO326_003397"
                     /pseudo
     CDS             complement(770..1702)
                     /locus_tag="OO326_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="OO326_003398"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="OO326_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5967667.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3991)
                     /locus_tag="OO326_003399"
     CDS             complement(2933..3991)
                     /locus_tag="OO326_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967668.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="OO326_003400"
     CDS             complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="OO326_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5967669.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6069..6353
                     /locus_tag="OO326_003401"
     CDS             6069..6353
                     /locus_tag="OO326_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5967670.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6533..7843
                     /gene="fadI"
                     /locus_tag="OO326_003402"
     CDS             6533..7843
                     /gene="fadI"
                     /locus_tag="OO326_003402"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5967671.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7843..9987
                     /gene="fadJ"
                     /locus_tag="OO326_003403"
     CDS             7843..9987
                     /gene="fadJ"
                     /locus_tag="OO326_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5967672.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10190..10675
                     /gene="sixA"
                     /locus_tag="OO326_003404"
     CDS             10190..10675
                     /gene="sixA"
                     /locus_tag="OO326_003404"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5967673.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2542 c         2744 g         3040 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagactttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggtgg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcggca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgctcggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctaaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatgctc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gtttgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcaaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgtctggc
     3001 gattttgaag tcttttttca actttatctt cgagattctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtcagttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt agatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctcaa
     3841 gtagatgttt aatacaggtg cttattcctt ccttaccatt aaaaaggttt ttgaactctt
     3901 gtactaatcc attaatttca tcattgttat tttcattgat ggattctggg taagctgcgc
     3961 tggtattgtt gattgaaata gatggtagca tgattttatt ccatgttgat tgatttgtta
     4021 caaatatata ttacctaatg aaatagacta tcaaaaaacg ctcatttatt tagaacctat
     4081 ctttaatttg agtgtataag cgcacaatag ccaggggggt tgagtatcct ggaaatgcag
     4141 aaaagtaaac tctttgagaa ggacgagcag aacgcttgaa aatatgcatt tcccttcagg
     4201 tttgtctgtg gataccactt actgatttga aagggcaagc tgccatcagg ctgtttgcaa
     4261 aactcaggag gcaagcaatg aggaaactac tttcggtgaa gtgggggagt taaagtcacc
     4321 tgcatagcag gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt
     4381 accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt taattttcac
     4441 gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta
     4501 agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg
     4561 agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta
     4621 gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa
     4681 cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta
     4741 agccaggcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca
     4801 catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg
     4861 aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa
     4921 gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata
     4981 caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt
     5041 attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc
     5101 aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat
     5161 tttcgcgcga gcgtagatgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg
     5221 atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc
     5281 gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc
     5341 ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg
     5401 cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc
     5461 cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc
     5521 ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg
     5581 gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga
     5641 gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac
     5701 cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa
     5761 gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca
     5821 gctagaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc
     5881 agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat
     5941 ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact
     6001 ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga
     6061 aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg
     6121 gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaaaccgcg
     6181 cagaagcaga acaaacgctg gcggcgctga ctgaaaaatc ccgtagcgtg aaatccgaac
     6241 cgtgcaaaat ctccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt
     6301 tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct
     6361 cgggtacgta cgcccctgtc agttgctggc aggggcgttt tcttttcttt cttctgtctt
     6421 cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt
     6481 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
     6541 ggttttacca ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
     6601 gccttttgcc cgccaggcga cggcttttca tggcattccc acggttgatt tagggaagat
     6661 ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt
     6721 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
     6781 taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
     6841 ccaagcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc
     6901 cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgtacgt
     6961 gctggttgat gtcaacaaag ctcgaaccat gagccagcgc ctgaaactct tctctcgcct
     7021 gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg
     7081 gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga
     7141 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
     7201 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
     7261 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
     7321 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
     7381 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
     7441 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac
     7501 accgctggcg ctggaacgtg ccggtttgac gatgagcgaa ctgacattga tcgatatgca
     7561 cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc
     7621 tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
     7681 gcttggcggt tcgattgctt acggacatcc cttcgcggcg accggcgcgc ggatgattac
     7741 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
     7801 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat ggcatcagcg
     7861 tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt accggatgag
     7921 aaaatgaata ccctgaaggc ggaatttgcc tcgcaggtgc gcgccattat taagcaactc
     7981 cgtgaaaaca aagagttgcg cggcgtggtg tttatctccg cgaaaccgga caattttatc
     8041 gccggagcag acatcaacat gattggcaac tgcaaaacgg cgcaagaagc ggaagcactg
     8101 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg
     8161 gctattcatg gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc
     8221 gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta
     8281 cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagtac agcattagag
     8341 atgattctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat
     8401 gacgttgttc cgcactccat tttgctggaa gccgctgttg agctggcgaa gaaggatcgc
     8461 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
     8521 ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg
     8581 acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggctat
     8641 gacgctgaag ctcgcgcctt tggcgaactg gcgatgacgc cgcaatcgca agctttgcgt
     8701 agtatctttt ttgccagtac ggacgtgaag aaagatcccg gtagtgatgc accgcctgcg
     8761 ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
     8821 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
     8881 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
     8941 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
     9001 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
     9061 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct
     9121 ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat
     9181 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
     9241 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc
     9301 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
     9361 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt
     9421 ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
     9481 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
     9541 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
     9601 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc gctcattggc
     9661 gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
     9721 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
     9781 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
     9841 gattctctcg gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt
     9901 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
     9961 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    10021 gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    10081 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    10141 tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    10201 tatcatgcgt cacggcgacg cagccctcga ttccgccagt gattcggttc gtcctctgac
    10261 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga
    10321 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    10381 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    10441 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    10501 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    10561 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    10621 tacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgtgt
    10681 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcattag gcagcggata
    10741 tctgtcaacc tcactactat cgaagactct ccttatctat aaataaaaca ctttctacat
    10801 tcattcggaa attattcttc tacgcgtatc ttgattttgc ccaaagttac atgttgaaat
    10861 gaataatatc tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag
    10921 ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata gtgtttatat
    10981 attatattgt caaaagtgga taaatgccaa tcgcattata tgttcattgt ttgtttttta
    11041 agctgttgat attcattatt gatatacttt ctattttttc taaatactta ttccgtattt
    11101 t
//
LOCUS       DAJTRW010000116        11049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRW010000116 DAJTRW010000000
VERSION     DAJTRW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..272)
                     /locus_tag="OO326_003405"
     CDS             complement(<1..272)
                     /locus_tag="OO326_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5967674.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO326_003406"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO326_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO326_003407"
     CDS             complement(1132..1338)
                     /locus_tag="OO326_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5967675.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO326_003408"
     CDS             complement(1445..2008)
                     /locus_tag="OO326_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5967676.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO326_003409"
     CDS             complement(3383..5218)
                     /locus_tag="OO326_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967677.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO326_003410"
     CDS             5319..5606
                     /locus_tag="OO326_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5967678.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO326_003411"
     CDS             5578..7107
                     /locus_tag="OO326_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5967679.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO326_003412"
     CDS             complement(7277..8494)
                     /locus_tag="OO326_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5967680.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OO326_003413"
     CDS             complement(8861..9847)
                     /locus_tag="OO326_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967681.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="OO326_003414"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="OO326_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10439..11049
                     /locus_tag="OO326_003415"
                     /pseudo
     CDS             10439..11049
                     /locus_tag="OO326_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT         3411 a         2558 c         2128 g         2952 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gcctgatctt
    10381 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgacttacat
    10441 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
    10501 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
    10561 gtatgaatca cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
    10621 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
    10681 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
    10741 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
    10801 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
    10861 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
    10921 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
    10981 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc
    11041 agcccggtc
//
LOCUS       DAJTRW010000117        11036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRW010000117 DAJTRW010000000
VERSION     DAJTRW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            409..1239
                     /locus_tag="OO326_003416"
     CDS             409..1239
                     /locus_tag="OO326_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5967682.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO326_003417"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO326_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5967683.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO326_003418"
     CDS             complement(2368..3828)
                     /locus_tag="OO326_003418"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5967684.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO326_003419"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO326_003419"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5967685.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO326_003420"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO326_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5967686.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO326_003421"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO326_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5967687.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO326_003422"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO326_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5967688.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO326_003423"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO326_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5967689.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11036)
                     /gene="fimD"
                     /locus_tag="OO326_003424"
     CDS             complement(9005..>11036)
                     /gene="fimD"
                     /locus_tag="OO326_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5967690.1"
                     /translation="NSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHI
                     ITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGI
                     ARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPY
                     SSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY
                     RAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVG
                     YRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQ
                     LGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQML
                     ALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQ
                     TGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTL
                     GQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADN
                     VDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIV
                     ADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2683 a         2820 c         2682 g         2851 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgtta
//
LOCUS       DAJTRW010000118        10945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRW010000118 DAJTRW010000000
VERSION     DAJTRW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO326_003425"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO326_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5967691.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO326_003426"
     CDS             complement(1433..1759)
                     /locus_tag="OO326_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5967692.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO326_003427"
     CDS             1894..2235
                     /locus_tag="OO326_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5967693.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO326_003428"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO326_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO326_003429"
     CDS             complement(2833..3543)
                     /locus_tag="OO326_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5967694.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO326_003430"
     CDS             3651..3956
                     /locus_tag="OO326_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5967695.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO326_003431"
     CDS             4155..4475
                     /locus_tag="OO326_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5967696.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO326_003432"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO326_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO326_003433"
     CDS             complement(6299..7288)
                     /locus_tag="OO326_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967697.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7861)
                     /locus_tag="OO326_003434"
     CDS             complement(7373..7861)
                     /locus_tag="OO326_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967698.1"
                     /translation="MMFNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLSG
                     LPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAER
                     MREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWMD
                     SL"
     gene            complement(8182..8682)
                     /locus_tag="OO326_003435"
     CDS             complement(8182..8682)
                     /locus_tag="OO326_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967699.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8672..9187)
                     /locus_tag="OO326_003436"
     CDS             complement(8672..9187)
                     /locus_tag="OO326_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967700.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9618..9986)
                     /locus_tag="OO326_003437"
     CDS             complement(9618..9986)
                     /locus_tag="OO326_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967701.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10398..>10945)
                     /locus_tag="OO326_003438"
     CDS             complement(10398..>10945)
                     /locus_tag="OO326_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5967702.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2810 a         2783 c         2704 g         2648 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgaacatca
     7861 taggtaattc tcctcttgag agttgagact atcggtatcg cagacgcagt agcgcctacg
     7921 ggtctccccg catcggtcat ctcaaaaggt ttaaaattag gtttggtatg gtcaggactg
     7981 cttagcaaag gctcggcgta actgattgtc taaatcacgg tccaccagca tagctatcag
     8041 ctcgtctcga ctgtagtaag ggcgctcagg gtcttcgact ccaagtctca gcagagacag
     8101 cagatgacgt gtgggcaggc cacggtaacg aagggtcgct ttgccgatag gtgtacgagg
     8161 gtcaatcata gccatgatta cttacctcca ctcacagcga ccagcggtca cagcttgata
     8221 gtctgaatac tcagcctgtg agacttcacg tccggcgatg tagaggcctg tgccatgacg
     8281 ctggatgaat tccttggtct ccggcttgat gtcctcagca gacgctacag cagactgcac
     8341 ggtggcgctg gaggtcatag acacgaaggc tcgggttact gccagtccgt acccaaggtc
     8401 caaccaccga ccgaactcta tagcggctac tgggtcagcc agccacgagg tccctaagtg
     8461 acccatgtta atctttttaa caaacccgtt ttctgggttg gttagacttc taccagcctt
     8521 ggtcctcatg aagcgagcaa cctcggtact acggttatct tctaccttac caatctgaat
     8581 gcctacccgg cgtatctcat ccagtcgaaa caatccgtcc tgactggtga tgtcctgacc
     8641 gaagatatga atggtgtatt cctgcttatt gtcagaagtc ataagaggca cctcccgcca
     8701 gagcttcggc aaaggacttg aagcgctgtt cttggtcagc tttcgcctga aggcgttcgg
     8761 catcaggtgg gaccagctta tcaaaggtgc cgttttcgta atcttcagtt aggacctgaa
     8821 ggcggcggga ccatttgctc tccacctgca tcatcgcgtt agagatgtgc ttcaagtcgg
     8881 catcagagcg tgacgctatc caatccatct cttctgagtc accctcacag gtgtaaggat
     8941 tgttgaaata gaagatgtcc tgcaggccac gcttcagctc ttcctcggtg tgtgtctgga
     9001 ggtatgactg gataggctcc gcgtacatct cccggatttt ctcatagtcc cagtcttccg
     9061 cctgggcata ccatcgggat aacttcagca ggtctaagtt atagcgacag caatcgacac
     9121 tggactctgc attggtcagc aggatgatta agaactggcg gttagttact ggtaattcgg
     9181 tattcataca aagatgctcc tttggtaaag cactttctgt ctccacatgg agtccgcacc
     9241 attcagacgc atcctaaacg gacccatcag aaagtgctta acgaaagtga atggttaaat
     9301 gttgacggtc tggagtgacc atgttctcca tgtacgtata aaggtgcgac cgcatgtcag
     9361 gacgagctgt aaggactcca gatggtgagc ggtgatagac ttaagccaat gtagtgagta
     9421 gacgagctac taaccgagac gactaaagaa cgctaacgag ctggaggtcg atacaggagg
     9481 tctgtcagag gcggtcacgg gccttgatta cttaggagag atacttaatg tcggttgacc
     9541 agatgctcgc atgttctgac gcatcagact cagaggcgga ggctggtctc cctatagtga
     9601 tagtttaggg tccgtaatca tgaaccagct ttactggcgt ggtactggac ggatttactg
     9661 gcgctgacga cggtagcata gacgtagcga gcgtagcgag ttgaaaccat aggttcaccc
     9721 aaggcaacac gcttacggct cagggactcc tgcacagcgc ttacagttaa tgtgtcgtgc
     9781 gttagtagtt gggtcagaat caagtatggg ctgacaggct tggtattgct tgccattgat
     9841 tcgttcaggc gaccagcacc agtaaggaca tcatccagcc aaccactcag gtcgccctga
     9901 ccatcgaatg acagcaggtc cccgcagtgc tgctgcaagt aggctttggt gaacgtgttg
     9961 tcgtgagttg cgattggtcg tgccatagag tatctcctgt agttgaatta gttggttccc
    10021 ctatagtgat aatcaagtcc atcactggtg atgcatacag tagcgcgaca ggatgtgact
    10081 cacaggttga accgaatgtc aaggactaaa gacgcctcag gaattaagga agtatatata
    10141 aagagagacc agaggcgact cacagttaaa caggaatgtc aagaggagac cataggaatt
    10201 tggagggact ggagtttagg acgcagttta aataagacca cagacagact tagagtcgga
    10261 cagccgaagg cggaccagtg ggcctatagt caggagggat tatgattatt atcaccctcc
    10321 ctaacttcag tcagacttca gtgacccaag gtctcaacca tcaagccgta tatggtctcc
    10381 accttgggtc tccctactta aagtaaagct gtcttaagca catcagccag cctacccaca
    10441 gcgtgtcctt tcccgtacag gtcatacgta attgactgac tgctgtgacc taagatgcct
    10501 tgagcgtcca ccagattgac tccacgctct ttcattcggg tcgccagcca atgtctcaga
    10561 ctgtggagag agacatcctg agagtctcct agggcctcag tcaggaccct tgagttaaac
    10621 cagctagagt aggcgctcgg agtcatcccg aagagaggac cctcatcagg ctgcatgtct
    10681 acccacgaca ggaaggacgt caggtcaaac ccataagcgc catcggttaa cggcacaagt
    10741 cggactgaat gtttgttctt cacagacttc ccgtccccat cttcattgat gtcgatacag
    10801 accagtccat tgtccaaggt tacaatgtca cgtttagtca ggtggcagac ctcagcagac
    10861 cgagcgccag tgaccacaga caccagagac gcccatctac gggcctcaga ggtcgtgtga
    10921 gccgtgtgtc gtgtgaactg atact
//
LOCUS       DAJTRW010000119        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRW010000119 DAJTRW010000000
VERSION     DAJTRW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            154..504
                     /locus_tag="OO326_003439"
     CDS             154..504
                     /locus_tag="OO326_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5967703.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO326_003440"
     CDS             557..952
                     /locus_tag="OO326_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5967704.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO326_003441"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO326_003441"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5967705.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO326_003442"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO326_003442"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5967706.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO326_003443"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO326_003443"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5967707.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO326_003444"
     CDS             complement(5323..6423)
                     /locus_tag="OO326_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5967708.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO326_003445"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO326_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5967709.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO326_003446"
     CDS             complement(7571..8137)
                     /locus_tag="OO326_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5967710.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO326_003447"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO326_003447"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5967711.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO326_003448"
     CDS             10263..10751
                     /locus_tag="OO326_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5967712.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2668 a         2788 c         2606 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa atagtcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJTRW010000120        10737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRW010000120 DAJTRW010000000
VERSION     DAJTRW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            195..1649
                     /gene="amn"
                     /locus_tag="OO326_003449"
     CDS             195..1649
                     /gene="amn"
                     /locus_tag="OO326_003449"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5967713.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1992..2708
                     /locus_tag="OO326_003450"
     CDS             1992..2708
                     /locus_tag="OO326_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5967714.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3164..3239)
                     /locus_tag="OO326_003451"
     tRNA            complement(3164..3239)
                     /locus_tag="OO326_003451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3204..3206),aa:Asn,seq:gtt)
     gene            complement(3340..4967)
                     /gene="yeeO"
                     /locus_tag="OO326_003452"
                     /pseudo
     CDS             complement(3340..4967)
                     /gene="yeeO"
                     /locus_tag="OO326_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4972..5047
                     /locus_tag="OO326_003453"
     tRNA            4972..5047
                     /locus_tag="OO326_003453"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5005..5007,aa:Asn,seq:gtt)
     gene            complement(5085..6035)
                     /gene="cbl"
                     /locus_tag="OO326_003454"
     CDS             complement(5085..6035)
                     /gene="cbl"
                     /locus_tag="OO326_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5967715.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6137..7054)
                     /gene="nac"
                     /locus_tag="OO326_003455"
     CDS             complement(6137..7054)
                     /gene="nac"
                     /locus_tag="OO326_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5967716.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7382..7457
                     /locus_tag="OO326_003456"
     tRNA            7382..7457
                     /locus_tag="OO326_003456"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7415..7417,aa:Asn,seq:gtt)
     gene            complement(7513..8448)
                     /gene="ldtA"
                     /locus_tag="OO326_003457"
     CDS             complement(7513..8448)
                     /gene="ldtA"
                     /locus_tag="OO326_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5967717.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8510..9589)
                     /gene="cobT"
                     /locus_tag="OO326_003458"
     CDS             complement(8510..9589)
                     /gene="cobT"
                     /locus_tag="OO326_003458"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5967718.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9601..10344)
                     /gene="cobS"
                     /locus_tag="OO326_003459"
     CDS             complement(9601..10344)
                     /gene="cobS"
                     /locus_tag="OO326_003459"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5967719.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10341..>10737)
                     /locus_tag="OO326_003460"
     CDS             complement(10341..>10737)
                     /locus_tag="OO326_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5967720.1"
                     /translation="RPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDY
                     GGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFR
                     DIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2999 a         2735 c         2317 g         2686 t
ORIGIN      
        1 gggaactggc atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc
       61 gctttattga tgaaaggcaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc
      121 gccttatact tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg
      181 aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag gcactggata
      241 aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata
      301 ttacaattgg cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat
      361 cactgaccgt aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc
      421 gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt
      481 atctgaatga gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac
      541 cctcgcaaca tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc
      601 gctcaatgag cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg
      661 atgaaactgc agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg
      721 cgcgccgcgt cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac
      781 attttcaacc gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt
      841 ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg
      901 ggaactggat cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga
      961 aaaaacacca gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg
     1021 tgaatattgg cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac
     1081 gcccggatat ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg
     1141 gagattatgt ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc
     1201 cgcccgatat acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca
     1261 agttagtcag cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg
     1321 taaccacaga tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact
     1381 taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt
     1441 tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta
     1501 agcttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg
     1561 gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac
     1621 gaacctttaa tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat
     1681 gtgaaggatt aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc
     1741 cttgggcaga tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat
     1801 gctttcaaaa cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat
     1861 ataagattat ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat
     1921 ataatatgcc gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg
     1981 agatcattcc agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg
     2041 gtgcaacgtc taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag
     2101 gtgaacccga tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg
     2161 cacaagttcc aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg
     2221 aaacgttcgt acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg
     2281 agacattgac ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa
     2341 aaggcggcaa tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga
     2401 ttgtatttaa aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg
     2461 atgttcgtga tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc
     2521 ttcataaagg aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat
     2581 tagaaatgat tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag
     2641 ggcttgttga tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa
     2701 atctctaatt atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa
     2761 ttcttataaa aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca
     2821 gagaaataat atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg
     2881 caattacgta tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc
     2941 tctttatctt tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc
     3001 tgttggggag gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca
     3061 gaaatcaaaa aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt
     3121 ttaactgtcc tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga
     3181 ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa
     3241 tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa
     3301 atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt
     3361 tttttcacac ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat
     3421 tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc
     3481 aaccacaccc cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca
     3541 catgctcaac atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc
     3601 gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat
     3661 cacaacgacg tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa
     3721 gggagccgtt agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg
     3781 caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt
     3841 agaagcagaa ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat
     3901 aaaatttccg gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg
     3961 accactggta aataacactg attcgacact cgctggaata ccaatcccca tgacttccca
     4021 gataatgcta aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt
     4081 aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa
     4141 acccaaccca gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata
     4201 ttaagaatat tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga
     4261 agtgccccgc taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc
     4321 agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata
     4381 atttgttcgc caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc
     4441 accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat
     4501 gctacaacga cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg
     4561 aagctgtccg ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa
     4621 gtgctcagga cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc
     4681 ggggttatct cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg
     4741 ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag
     4801 tgaaaccaca tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca
     4861 aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa
     4921 atcgggtttg acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta
     4981 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     5041 ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca
     5101 atttcctctt cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca
     5161 ttacaaagtt ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc
     5221 aaccagacag tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc
     5281 tcttgttcgc cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag
     5341 gttttaatga catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg
     5401 gtaaatgcgt catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc
     5461 cacttcgcta ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga
     5521 acaagcaaac tatggtgcca acaaaaccat gggaaggcga cgagctgcgg gtcattactc
     5581 aaacgctcgc tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc
     5641 tgtggcgaac cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta
     5701 atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca
     5761 gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata
     5821 cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct
     5881 cgtcgaacaa atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg
     5941 cctgactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca
     6001 gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat
     6061 cccgttattg ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc
     6121 catcccgata taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac
     6181 taatcactga cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg
     6241 cgggtaaatt taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc
     6301 catttactgc gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc
     6361 cgctggcaat cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg
     6421 tgagccgccg tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg
     6481 ggagaaaaag gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt
     6541 gagttcctac caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag
     6601 gggaatgctc ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca
     6661 ctgcaccact attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct
     6721 gtaataaggg catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt
     6781 gccccgataa tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta
     6841 aaatggcccg tgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt
     6901 ttgtgcgaat caaaagctgt tgatttaact caccttccag tatggcaacc tgttggctga
     6961 gcgctggttg tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa
     7021 tttttacgaa gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc
     7081 ccaagtgccg ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt
     7141 tgatgcgcaa gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt
     7201 tttatcagca gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat
     7261 tacccaataa gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa
     7321 atgcatttca ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga
     7381 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     7441 tccagtcaga ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa
     7501 ttaccaacgc tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga
     7561 tcgatgttgc aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc
     7621 ccgcaaagat ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt
     7681 gcgcgacagc ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt
     7741 cactggctga tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt
     7801 gatatcgtca ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt
     7861 ggcattggta ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg
     7921 gttatcgggc cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg
     7981 ccggatgttc ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac
     8041 ccagttacgc ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt
     8101 attactgtct ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt
     8161 acgaacagtg gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg
     8221 caaaaaaaca tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg
     8281 tgcggcaaaa gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg
     8341 ccccactaaa cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct
     8401 ggcaaaaagc agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta
     8461 agtcgcgaat actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca
     8521 aatcagaagt cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt
     8581 tgtatatcgc acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac
     8641 ctaaacgcat atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg
     8701 cgcctttttc tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct
     8761 ggcaggctgc gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc
     8821 aggaagcagc acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg
     8881 ccaggacatc aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa
     8941 catcaatttt attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt
     9001 caggatcccg gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc
     9061 ccagttcacc tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata
     9121 tgacgtccaa aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat
     9181 tgccgctacc tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa
     9241 taccaacatc aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg
     9301 ttgttccacg ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga
     9361 ccccttcctc ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat
     9421 gcggtatgcc attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc
     9481 ctaggctacc aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg
     9541 cagaattgat ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct
     9601 ttatagcagt gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc
     9661 gcccgtttgc ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat
     9721 cgtcaccacc atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc
     9781 ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc
     9841 aagcccttct tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct
     9901 gaccgcacat gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct
     9961 taacaccaga atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa
    10021 acggctatca cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc
    10081 acaggtatca gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac
    10141 gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc
    10201 gctaatcgcg ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg
    10261 ttcgaaatcc agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag
    10321 catcgcccaa aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat
    10381 acttcatttg ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt
    10441 cgtgccagac gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc
    10501 tttgcggggc aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt
    10561 tccatcgcct gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga
    10621 ttagtcacca ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca
    10681 tgaattaatt catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcgg
//
LOCUS       DAJTRW010000121         9992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRW010000121 DAJTRW010000000
VERSION     DAJTRW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..134
                     /locus_tag="OO326_003461"
     CDS             <1..134
                     /locus_tag="OO326_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5967721.1"
                     /translation="NLLIIESFGKMAYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            124..297
                     /locus_tag="OO326_003462"
     CDS             124..297
                     /locus_tag="OO326_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5967722.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(346..1419)
                     /locus_tag="OO326_003463"
     CDS             complement(346..1419)
                     /locus_tag="OO326_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5967723.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1521..4232)
                     /gene="torS"
                     /locus_tag="OO326_003464"
                     /pseudo
     CDS             complement(1521..4232)
                     /gene="torS"
                     /locus_tag="OO326_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4315..5343
                     /gene="torT"
                     /locus_tag="OO326_003465"
     CDS             4315..5343
                     /gene="torT"
                     /locus_tag="OO326_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5967724.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5316..6008)
                     /gene="torR"
                     /locus_tag="OO326_003466"
     CDS             complement(5316..6008)
                     /gene="torR"
                     /locus_tag="OO326_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5967725.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6095..7267
                     /gene="torC"
                     /locus_tag="OO326_003467"
     CDS             6095..7267
                     /gene="torC"
                     /locus_tag="OO326_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5967726.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7267..9807
                     /gene="torA"
                     /locus_tag="OO326_003468"
                     /pseudo
     CDS             7267..9807
                     /gene="torA"
                     /locus_tag="OO326_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9804..>9992
                     /gene="torD"
                     /locus_tag="OO326_003469"
     CDS             9804..>9992
                     /gene="torD"
                     /locus_tag="OO326_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5967727.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2405 a         2575 c         2686 g         2326 t
ORIGIN      
        1 ataacctgtt gattattgaa tctttcggga agatggctta taacatttct tacctgacca
       61 gggtaccggg aaccaacacc ttactggcgt gttgctgtct ttcaagacca gacgaggtta
      121 acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg
      181 caaaaaatcg ccgagctgaa caagaataca gggaaagaag tctctgaaat tcgcttcatc
      241 gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt
      301 tgtgaacatc accccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat
      361 tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc
      421 cgcatacatt gcgtaaccaa tcaccgcaaa cataatcccc agaaccagta gcgtcattaa
      481 ccagccagac aacgcaaagg cttttttatt tgctgcaggt ttttgcagtg aaaaggtcaa
      541 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
      601 tacctgaatt aatttatcta ccgggatccg tgatgggcac ttttttgcgc atttgccaca
      661 atcgatacaa ctttcggcat tgcgacgaat cttaaacggc gacaatagcg aaaccacgcc
      721 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
      781 aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag
      841 cattttaacg tccatcacca cgctataggg cgacaacata aaatagtgaa tcgcctgagc
      901 gggcatcaat aacgcgatat agagaaataa actcaacagc aaatacttca cgccgcgcag
      961 aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc
     1021 gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag
     1081 taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg
     1141 taactgacct gttgcaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc
     1201 ttcgatacct cccggtctgg cgacaaatgt cgtactacct gccgtttcgt aatagcgcac
     1261 ccaataccag aacgtgatgg caatataaat attcattgcc aatagtaaga attgcgtcgc
     1321 tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcgtgcc
     1381 tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag tccgttaagt
     1441 tgcataccaa atgccactat tctagttatt cttaactggc tgatattgat tcaaatcgcg
     1501 ttcaggtctt tcttattcaa tcatgcttcc agagcggcaa cactgcgtgt aatttcttcg
     1561 tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg actggcgcta
     1621 cgcattccca gactcgagca actgcttttt agctgatgcg cggcacgctt tattttctcg
     1681 ctgtcctggc tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc atgttgttta
     1741 aataatgcca gccattcgtg gatcttctcc gtccccatta actgagcgtc ttcattgagt
     1801 tgcgagacat ccagtggtag atcgttattg gcttgcagtt ggagatagtg cgccagtaac
     1861 tgaccgagca cttcacgcgg caccggttta gggataatcc cgcgaaatag tgaactggta
     1921 cgctggcgca gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac cagcgacgga
     1981 tattgctgtg ccagttgccg ggcaagggta atgccgtcga tatccggcag atcaaaatcc
     2041 accagtgcgg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg
     2101 ccaatagcaa caacctgcgc accactggtg ttcagcatct caacggtaat tcgctgggtt
     2161 agcgggttat cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc ctgattggct
     2221 gtttttggca cgggtgcagt ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca
     2281 acctccggcg tgctggtggc gctcagttcg ccgcccatcg cctgggccag acggctactg
     2341 atagtcagcc ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa tggctggaag
     2401 atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat
     2461 tgctcgccat cagtgcgact acgcaggata atgtgccctt cgtcagtaaa acgcagggca
     2521 ttgctcaata ggttggtaat gacctgacga atacgtcgtg gatcgcccat taacgcgctc
     2581 ggtacatcat cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccgctc
     2641 attaattgca gggtactttc cagcagcggg cgcggttcaa agggctcatc gctgactgaa
     2701 acatttttgc cacctgcttc gatagccgaa taatcgagaa tatcgttgag gatggtcagc
     2761 aacgattcgc cagagtcggt aatcgcctgc aaatcatcac gctgggcgtt aagtgcgggg
     2821 ttatctgcca gcagttgagc agtgccgaga ataccgtaca gcggtgtgcg gatctcatgg
     2881 ctcatcgccg tcagaaacgc cgattttgcc tggctggctt tttctgcttc cgcccgtgcc
     2941 tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc
     3001 agctgttcgc ggtggcgatt cagtgcatga acactgctgc gaaacgcatc catcagccgc
     3061 ccgatggtat ccagctcccg tacgccagcg gtttccggga aaggggagtc aatatcaccg
     3121 tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgaa tgagcgataa
     3181 accacgcgcc agaggatcat aatcagtgcg caaagtgaaa ccatccccag caataacagg
     3241 ctgtattgcc cgcgtgcact ggctttttcc agatgtgcca gtccatgctg attgtgcagc
     3301 tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg ggcgatatta
     3361 ttttgggcga gagtttgcag gtgattgctg atttcactgt cctgctggaa cagcgccaac
     3421 agatcgctat attggctaac ggtagttaac gttgttgcga cctgcgcacg aactcccgga
     3481 tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc
     3541 agcgttggtg catttttctg gatctgctcc agccccagat tcatcaccat ttgctgcacc
     3601 cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc
     3661 agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa atcgtaaatc
     3721 ccggcctggg tcgctccagc ggaagttgtc gcattattcg cctgaccttg ctccaggcgt
     3781 gcgatctcat cggcggcagc tatctgctga ctgagttgcc gttgttgctg gcgtagttgc
     3841 agacgccgcc ccaccagttc cccttgctga cgtaatgaac gggagatctc ctgctcctgt
     3901 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     3961 ttcaggcttt atgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4021 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4081 atattcatgg taggaatcaa tgcctgagta ttgtcttttt ccacctggct gataaagcgc
     4141 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4201 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cacttaaggt gaaaaaggtt
     4261 gagtcgcaaa gtggaatgca tctagcataa agccttatta ttgatgaggc tatcatgcgc
     4321 gtactgctat ttttacttct ttcccttttc atgttgtcgg cattttcggc tgataacctg
     4381 ttgcgctggc atgatgcgca gcatttcacg gtgcaagcct ctatgccgct taaagccaaa
     4441 cgcgtatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4501 aactatggta tgcaggaggc tgctcgccgc tacggtgtgg atttaaaagt gctggaggca
     4561 ggcggctaca gccagttggc tacccagcaa gcacaaatcg accagtgtaa acagtggggc
     4621 gcagaggcca ttttgctcgg tagtagcacg acgtcatttc ccgacctgca aaagcaggta
     4681 gcaaatctgc cggtgatcga actggtaaat gctcttgatg atccccaggt gaaaagccgc
     4741 gttggtgtac cctggtttca gatgggctat caacctgggc gatatttggt gcaatgggcg
     4801 cacggtaaac cactgaacgt gctgctgatg cccggacccg ataacgccgg gggcagtaag
     4861 gagatggtcg agggttttcg cgcagccatt gccggaagcc cggtgcgtat tgttgatatt
     4921 gcgcttggtg ataacgatat tgaaatccag cgcaacctgt tgcaggagat gctggaacgc
     4981 cacccagaaa tcgacgtcgt tgccggaacg gccattgcgg cagaggcggc aatgggtgaa
     5041 gggcgtaacc tgaaaacgcc gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat
     5101 cgcgggctga agcggggaag agtgattatg gctgccagcg atcaaatggt ctggcagggg
     5161 gaactggcag ttgagcaggc catcaggcaa ttacaggggc aatcggtgtc tgataatgtc
     5221 agcccaccga ttttagttct gacgccgaaa aatgccgacc gagaacatat ccgccgctcg
     5281 ctgtcaccgg ggggatttcg tccggtctat ttttatcagc acacatcagc ggctaagaaa
     5341 taaccttcac catgttgcgt caccagtaaa tccgcgctga gtttatgacg taaacgacga
     5401 attaacacat cgacggtgcg caggtcaggg ttttccaccc gacgcgcaga aagcatacgc
     5461 agcagacgct cacggctgag aatttcgccc ggattagtca caaatgccac caacatttca
     5521 tactctgcgc gggtcagttt aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca
     5581 ttcaggcaat aacccgcaaa gcgatagcag tagtcctgag tgtgcggttg agcttgtcgc
     5641 gcgaggtcga ttcgccagag cagatttttc acccgtacta ccagttcgcg cagttccagc
     5701 ggtttggtga cgtaatcgtc tgcgcccatt tccagcccaa caatacggtc aatccgatcg
     5761 ctgcgtccgg taaccagaat aatccccacc gttgagcgtt ctcgcagggc gcgggttaac
     5821 atcaggccat tttcatcggg taagttgata tccagcagaa ttaaatctac cggctgattc
     5881 tgcataattt cccgtagccc ggcaccgctc gccgtaacgg aaacggtata cccctcctga
     5941 gtgaagtagg attgtaatcg cgcctgggta accggcttat cttcaacaat aacaatgtga
     6001 tgtggcatca gagggtttta ctcattctgt tcatatctgt tcatatttgc tcattaagat
     6061 cgcttcacta aaccataatt ctacaggggt tattatgcgg aaactctgga acgcgctacg
     6121 ccgacccagt gctcgttggt cggtactggc gctggtcgca attgggattg tgattggcat
     6181 tgcgctgatt gtattgccac acgttgggat caaagtcacc agcacaaccg aattttgtgt
     6241 cagttgccac agtatgcaac cggtgtatga agaatataaa cagtcggtgc atttccagaa
     6301 cgcctccggc gtgcgagctg aatgccatga ctgtcatatc ccgccggata ttccaggcat
     6361 ggtgaagcgc aaactggaag cgagcaacga catttaccag acctttattg cccactccat
     6421 tgatacacct gaaaaattcg aagccaaacg cgcggaactt gccgagcgtg aatgggcgcg
     6481 aatgaaagaa aacaactcgg caacctgtcg ctcctgccat aactacgatg cgatggatca
     6541 tgccaggcag catcctgaag cggcacgtca gatgaaggtg gcagcgaaag ataatcaatc
     6601 ctgcatcgac tgtcataaag gtattgccca ccagttaccg gatatgagta gcggcttccg
     6661 taagcagttc gatgagctgc gcgccagtgc taatgacagt ggtgacacgc tgtactctat
     6721 tgatattaag ccaatttatg cggcgaaagg cgataaagaa gcctctggtt ctctgctgcc
     6781 tgcttcggaa gtgaaagtcc ttaaacgtga cggcgactgg ctgcaaattg aaattaccgg
     6841 ctggacggaa agcgccggac gtcagcgtgt actcacccaa ttcccaggta aacgcatctt
     6901 tgttgcctcg attcgtggtg atgtgcagca gcaggtgaaa acgctggaga aaaccaccgt
     6961 tgccgacacc aataccgagt ggagcaagtt gcaggccacc gcgtggatga agaaaggcga
     7021 catggtgaac gatatcaaac cgatctgggc ctatgcggat tcgttgtaca acggcacctg
     7081 taacgagtgc cacggcgcac cggaaatcgc ccactttgac gctaacggtt ggatcggcac
     7141 gctcaacggc atgattggct ttaccagtct cgataaacgt gaagaacgca ctttgttgaa
     7201 atatctgcaa atgaatgcgt ctgacaccgc aggtaaggct cacggcgata agaaggaaga
     7261 aaaataatga acaataacga tctctttcag gcatcacgtc ggcgttttct ggcacaactc
     7321 ggcggcttaa ccgtcgccgg gatgctgggg ccgtcattgt taacgccgcg ccgtgcaact
     7381 gcggcgcaag cggcgactga ggctgtcatc tcgaaagagg gcattcttac cgggtcgcac
     7441 tggggggcta tccgcgcgat ggtgaaggat ggtcgctttg tggcggcaaa accgttcgaa
     7501 ctggataaat atccgtcgaa aatgatcgcc ggattgccgg atcacgtaca caacgcggca
     7561 cgtattcgtt atccgatggt acgcgtggac tggctgcgta agcgccatct gagcgacacc
     7621 tcccagcgcg gtgataaccg ttttgtgcgc gtgagctggg atgaagccct cgacatgttc
     7681 tatgaagaac tggaacgcgt acagaaaact cacgggccga gtgccttgct gaccgccagt
     7741 ggttggcaat cgacggggat gttccataac gcttcgggga tgctggcgaa agctattgcc
     7801 ttgcatggta atagcgttgg tacgggcgga gattactcta ccggtgctgc gcaggtgatc
     7861 ctgccgcgcg tagtcggttc aatggaagtg tatgaacagc aaacctcctg gccgctggta
     7921 ttgcagaaca gcaaaaccat tgtgctgtgg ggctccgatt tgctgaaaaa ccagcaagcg
     7981 aactggtggt gcccggatca cgatgtttat gaatattacg agcagctaaa agcgaaagtc
     8041 gccgccggtg aagttgaggt catcagcatc gatccggttg tcacatccac ccatgagtat
     8101 ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc aaactgacgt gccgctgcaa
     8161 ctggcgctgg cacatacgct gtacagtgaa aacctgtacg acaaaaactt cctcgctaac
     8221 tactgtgtgg gttttgagca gttcctgccg tatctgctgg gtgagaaaga cggtcagccg
     8281 aaagatgccg catgggctga aaaactgacc ggcattgatg ccgaaaccat tcgtgggctg
     8341 gcgcggcaga tggcggcgaa cagaacgcaa attattgctg gctggtgcgt gcagcgtatg
     8401 cagcacggtg aacagtgggc gtggatgatt gtggttctgg cggcgatgct ggggcaaatt
     8461 ggcctgccag gtggtggttt tggctggcac tacaacggcg caggcacgcc ggggcgtaaa
     8521 ggcgttattc tgagtggttt ctccggctct acgtcgattc cgcctgttca cgacaacagt
     8581 gactacaaag gctacagcag cactattccg attgcccgtt ttatcgatgc gatcctcgaa
     8641 ccggggaaag tgatcaactg gaacggtaaa tcggtaaaac tgccgccgct gaaaatgtgt
     8701 atttttgccg gaactaaccc attccatcgc catcagcaga tcaaccgcat tattgaaggc
     8761 tggcgcaagc tggaaacggt tatcgccata gataaccagt ggacctcaac ctgccgcttt
     8821 gccgatatcg tgctgcctgc gaccacgcag tttgagcgta acgatctcga ccagtacggc
     8881 aaccactcca accgtggcat tatcgccatg aaacaggtgg tgccgccgta gttcgaggcg
     8941 cgcaacgact ttgatatttt ccgcgagctg tgccgtcgct ttaatcgtga agaagccttt
     9001 accgaagggc tggacgaaat gggctggctg aaacgcatct ggcaggaagg tgtacaacaa
     9061 ggcaaaggac gcggcgttca tctgccagcg tttgatgact tctggaataa caaagagtac
     9121 gtcgagtttg accatccgca gatgtttgtt cgccaccagg cattccgcga agatccggat
     9181 ctcgaaccgc tgggcacgcc gagtggcctg attgagatct actcgaaaac catcgccgat
     9241 atgaactacg acgattgtca ggggcatccg atgtggtttg agaaaatcga acgctcccac
     9301 ggtgggcccg gctcgcagaa gtatccgttg catctgcaat ctgtgcatcc ggatttccga
     9361 cttcactcgc agctatgtga gtcggaaacg ctgcgtcagc aatatacggt agcgggtaaa
     9421 gagccagtgt tcattaaccc gcaggatgcc agcgcgcgcg gtattcgtaa cggtgatgtg
     9481 gtacgcgtct ttaacgctcg cggtcaggtg ttggcaggag cagtagtttc tgaccgctat
     9541 gcacccggcg tggcgcgaat tcacgaaggg gcatggtacg atccagataa aggcggcgag
     9601 cctggtgcgc tgtgcaaata cggtaacccc aacgtgctga ccatcgacat cgggacttcg
     9661 cagctggcgc aggcgaccag tgcgcacact acgctggtgg aaattgagaa gtacaatgga
     9721 acagtggagc aggtgacggc gtttaacggc cccgtggaga tggtggcgca gtgcgaatat
     9781 gttcccgcgt cgcaggtgaa atcatgacca cgctgacagc acaacagatt gcctgtgttt
     9841 acgcctggct ggcgcagttg ttctcccgtg aactggacga tgaacaactg acgcaaatcg
     9901 ccagtgcgca gatggctgaa tggttttcgt tgctgaaaag cgaaccgccg ctcactgcgg
     9961 cggtgaatga tctggaaaac agtattgcca cg
//
LOCUS       DAJTRW010000122         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRW010000122 DAJTRW010000000
VERSION     DAJTRW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO326_003470"
     CDS             complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO326_003470"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5967728.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQI"
     gene            complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO326_003471"
     CDS             complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO326_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5967729.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO326_003472"
     CDS             complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO326_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5967730.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO326_003473"
     CDS             complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO326_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5967731.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO326_003474"
     CDS             complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO326_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5967732.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO326_003475"
     CDS             complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO326_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5967733.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO326_003476"
     CDS             complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO326_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5967734.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO326_003477"
     CDS             complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO326_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5967735.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8980..>9925)
                     /locus_tag="OO326_003478"
     CDS             complement(8980..>9925)
                     /locus_tag="OO326_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5967736.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2559 a         2582 c         2292 g         2492 t
ORIGIN      
        1 tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat attcagtatg
       61 ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt catgtaataa
      121 ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc agtcatctca
      181 atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg gccacgcgcc
      241 tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa catatcctgt
      301 ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat tttcgcttcg
      361 gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg atgatggatc
      421 gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt gaatggcgcg
      481 tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg ttggtagcgg
      541 gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt agcccaaccg
      601 ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac cagcggcttg
      661 atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc
      721 ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat acgcagacag
      781 gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata aaaatcgatg
      841 gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc cattacgcca
      901 tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc accttccacc
      961 tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt cataattaac
     1021 tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat ctgcgccgtg
     1081 ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg cgtcgtcaga
     1141 tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc gcctgtattg
     1201 accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg
     1261 ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat ggcgtgtaat
     1321 gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc aaccgagggc
     1381 agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc gacgtgaatg
     1441 agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg tggtgtaata
     1501 acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact ggtgatgaaa
     1561 tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc aataacgctg
     1621 gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt gaaaatactt
     1681 ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc ccccaatgct
     1741 ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc atataccgct
     1801 ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac ttttttctgc
     1861 gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat aatggcgggc
     1921 attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat acacagcggc
     1981 accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc cattaccgcg
     2041 cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat catttcgctt
     2101 atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac catcagacat
     2161 agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag tcggcgttcc
     2221 agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga gtaattcaac
     2281 aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg cccgttctcg
     2341 aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc agtaaatgga
     2401 ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa cccaaacaag
     2461 gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt ccactgaaaa
     2521 gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag accgatcaga
     2581 ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc tttctcatca
     2641 ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac cgccaggaag
     2701 acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc caccaccata
     2761 ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc ctttaactta
     2821 aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc cccgactcct
     2881 gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg cggcaaaact
     2941 ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt tcaggttttg
     3001 cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt ctttgcgcac
     3061 gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac gttgatactg
     3121 caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt
     3181 tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca
     3241 ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt
     3301 aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc
     3361 aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac
     3421 ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc
     3481 tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt tttgctgaaa
     3541 gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt cctgtaattt
     3601 tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca cctcaagagg
     3661 aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca aggcatactc
     3721 tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc caattccgcg
     3781 ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat tattgttgag
     3841 aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa aaaaaccgac
     3901 ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac aatccagtca
     3961 tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga
     4021 gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt aactttgtgt
     4081 aattttagga atttataaag ttatatataa caaatcccaa taattaagtt attgggattt
     4141 gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc tcatcaccgc
     4201 ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa atattctggc
     4261 agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt acgctcggaa
     4321 tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct tcgtattcct
     4381 gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc atccgcgcga
     4441 acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac tccagactta
     4501 ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag attttgtccg
     4561 ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata accagtcgca
     4621 ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca tcgatcgcca
     4681 cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga tcgctgtcct
     4741 ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg cccatggtca
     4801 tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata tgtttattaa
     4861 gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga tgtagtcttc
     4921 tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact caatcaattc
     4981 gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt
     5041 gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa tacgcccggt
     5101 agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg ggcgaatggc
     5161 gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt aaccgctctg
     5221 gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc actgcacgcg
     5281 ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg cgatgttgtc
     5341 gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt tcgcacgcag
     5401 cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa tttcaccttc
     5461 cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca gcgtcgattt
     5521 accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat ccaggttgat
     5581 gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct gaattttact
     5641 cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga tgcttttcgc
     5701 taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa aagtgttaac
     5761 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg acgcacaggg
     5821 taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg ctcatcgcaa
     5881 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
     5941 ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg gcaatcgcca
     6001 gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg gagatcatct
     6061 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
     6121 gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc gcaattacgc
     6181 ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca aacagaccaa
     6241 ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
     6301 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
     6361 ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc agaccaccac
     6421 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccgtcgatac
     6481 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag gccatcgtcg
     6541 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
     6601 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
     6661 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
     6721 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
     6781 aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg aggcgctgtc
     6841 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
     6901 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
     6961 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
     7021 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
     7081 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
     7141 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
     7201 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
     7261 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
     7321 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
     7381 gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg tccataggaa
     7441 agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga caataatgcc
     7501 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
     7561 gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt actcttcagt
     7621 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
     7681 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
     7741 gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt
     7801 ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc ttgttctggt
     7861 ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
     7921 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
     7981 ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg
     8041 tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc acccggcaga
     8101 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
     8161 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
     8221 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa
     8281 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
     8341 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
     8401 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
     8461 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
     8521 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
     8581 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
     8641 cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca
     8701 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
     8761 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tataaggaat
     8821 atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa atgtgaagat
     8881 gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa gaaatgggtg
     8941 agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa tctcaacagt
     9001 tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa ggctcgcagg
     9061 agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt tatccatgtc
     9121 tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt aaatttttat
     9181 ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt cggtagcata
     9241 aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact caatagtgac
     9301 tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa aatctgcagc
     9361 tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc aactttgcgg
     9421 agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat ccaccggggt
     9481 gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa tcaattttcc
     9541 tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg atatacttaa
     9601 aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag catgatattc
     9661 aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa catcaaggtt
     9721 cggattaaga tcataataat taatattaga gtgaacccca gcataagtta atgcctggtt
     9781 tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat cacaatgaca
     9841 gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc caaccttatt
     9901 ttcgttcgcg gtaataagat atccc
//
LOCUS       DAJTRW010000123         9895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRW010000123 DAJTRW010000000
VERSION     DAJTRW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO326_003479"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO326_003479"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5967737.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO326_003480"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO326_003480"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5967738.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO326_003481"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO326_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5967739.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4682..6442)
                     /locus_tag="OO326_003482"
     CDS             complement(4682..6442)
                     /locus_tag="OO326_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5967740.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6572..7198
                     /locus_tag="OO326_003483"
                     /pseudo
     CDS             6572..7198
                     /locus_tag="OO326_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7397..8485
                     /gene="serC"
                     /locus_tag="OO326_003484"
     CDS             7397..8485
                     /gene="serC"
                     /locus_tag="OO326_003484"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5967741.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8556..9839
                     /gene="aroA"
                     /locus_tag="OO326_003485"
     CDS             8556..9839
                     /gene="aroA"
                     /locus_tag="OO326_003485"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5967742.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVAGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2518 a         2368 c         2478 g         2531 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa
     4441 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
     4501 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
     4561 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
     4621 aaacgccccg taatataact cagactaatc attaagccta cattgcgtag gctatttgat
     4681 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
     4741 actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg
     4801 cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa
     4861 acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt
     4921 gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg
     4981 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
     5041 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
     5101 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
     5161 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
     5221 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
     5281 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
     5341 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
     5401 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
     5461 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
     5521 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
     5581 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     5641 cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     5701 catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg
     5761 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     5821 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
     5881 tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     5941 aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt
     6001 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     6061 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc
     6121 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     6181 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     6241 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     6301 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     6361 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
     6421 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     6481 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     6541 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     6601 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     6661 tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg
     6721 tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat
     6781 gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg
     6841 gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga
     6901 gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat
     6961 tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg
     7021 tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga
     7081 ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg
     7141 tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca
     7201 gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca
     7261 tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag
     7321 ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc
     7381 aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc
     7441 ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc
     7501 ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa
     7561 ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct gccatggcgg
     7621 tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga
     7681 ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac
     7741 gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg
     7801 tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga
     7861 cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt
     7921 ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg
     7981 cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct
     8041 gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg
     8101 ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg
     8161 gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact
     8221 gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg
     8281 ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga
     8341 agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg
     8401 cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt
     8461 tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg
     8521 tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca
     8581 tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt
     8641 tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg
     8701 acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg
     8761 atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg
     8821 agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg
     8881 atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc
     8941 tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc
     9001 cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgct ggctccgttt
     9061 ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga
     9121 ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga
     9181 agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc
     9241 agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact
     9301 tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca
     9361 gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct
     9421 ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga
     9481 accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca
     9541 ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg
     9601 caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca
     9661 ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga
     9721 tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca
     9781 cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa
     9841 tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca cattg
//
LOCUS       DAJTRW010000124         9764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRW010000124 DAJTRW010000000
VERSION     DAJTRW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            382..2013
                     /gene="oppA"
                     /locus_tag="OO326_003486"
     CDS             382..2013
                     /gene="oppA"
                     /locus_tag="OO326_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5967743.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2099..3019
                     /gene="oppB"
                     /locus_tag="OO326_003487"
     CDS             2099..3019
                     /gene="oppB"
                     /locus_tag="OO326_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5967744.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3034..3942
                     /gene="oppC"
                     /locus_tag="OO326_003488"
     CDS             3034..3942
                     /gene="oppC"
                     /locus_tag="OO326_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5967745.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3954..4967
                     /gene="oppD"
                     /locus_tag="OO326_003489"
     CDS             3954..4967
                     /gene="oppD"
                     /locus_tag="OO326_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5967746.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4964..5968
                     /gene="oppF"
                     /locus_tag="OO326_003490"
     CDS             4964..5968
                     /gene="oppF"
                     /locus_tag="OO326_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5967747.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6021..6350)
                     /locus_tag="OO326_003491"
     CDS             complement(6021..6350)
                     /locus_tag="OO326_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5967748.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6385..7845)
                     /gene="cls"
                     /locus_tag="OO326_003492"
     CDS             complement(6385..7845)
                     /gene="cls"
                     /locus_tag="OO326_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5967749.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7988..8161
                     /locus_tag="OO326_003493"
     CDS             7988..8161
                     /locus_tag="OO326_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5967750.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8216..9469)
                     /gene="kch"
                     /locus_tag="OO326_003494"
     CDS             complement(8216..9469)
                     /gene="kch"
                     /locus_tag="OO326_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5967751.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2559 a         2286 c         2430 g         2489 t
ORIGIN      
        1 ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga gaatcgctta
       61 acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc gggcaaccgc
      121 cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata
      181 atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca
      241 gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca gcagaaagtc
      301 tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat
      361 aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc
      421 gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc
      481 acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca gtcattagat
      541 ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta
      601 ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg ggataataaa
      661 gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga tggcacgcca
      721 gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct
      781 ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg
      841 aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt agaagtcacc
      901 ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg
      961 ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc
     1021 aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc
     1081 ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct
     1141 tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac gtataacaac
     1201 atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat
     1261 ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg
     1321 cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa
     1381 gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg cgcaaaattg
     1441 actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg
     1501 ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg gtataacacc
     1561 tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt
     1621 gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggc
     1681 acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac ttccttcctg
     1741 aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac
     1801 agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact
     1861 aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat
     1921 gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc
     1981 tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct
     2041 gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat
     2101 gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta ttcttattac
     2161 tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg aacgtacttt
     2221 accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac
     2281 acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat cgtttaaata
     2341 taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg ccaaactggg
     2401 agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt
     2461 aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat
     2521 ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt tgcattggct
     2581 gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt
     2641 gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt
     2701 acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt
     2761 acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt
     2821 cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg ggattgggca
     2881 actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct
     2941 ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga
     3001 cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag
     3061 acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca
     3121 cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct ggtgctgatc
     3181 gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg
     3241 gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc
     3301 ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat ggtcggtgtt
     3361 gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc cggttatctg
     3421 ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc
     3481 atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct gattttcgtg
     3541 gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt
     3601 ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac gccgggcatt
     3661 gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc atcgctactg
     3721 gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag
     3781 ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga agtctctcca
     3841 tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt caactttatc
     3901 ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg
     3961 taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga
     4021 aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttaa
     4081 atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt tcgggtaaat
     4141 cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt ggcggatcgg
     4201 caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat aaactgcgcg
     4261 ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg
     4321 tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt
     4381 ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga
     4441 aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt gcgatggcat
     4501 tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg
     4561 tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat actgccatca
     4621 ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt
     4681 acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa cccgttcatc
     4741 cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt gaaacaatgt
     4801 tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc ccgttccagc
     4861 cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc
     4921 ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaactga
     4981 aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa tcaaagatgg
     5041 caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcactcttcg
     5101 cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt
     5161 tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct ggttaggtaa
     5221 agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat
     5281 tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga tcatcgctga
     5341 accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc
     5401 gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc
     5461 cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat
     5521 tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg tggttaacct
     5581 gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc
     5641 cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cagtagaact
     5701 ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc
     5761 ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg
     5821 ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat
     5881 tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca gacacgccgt
     5941 ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg tcagcccttt
     6001 ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt
     6061 tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc actgtccgcc
     6121 agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc
     6181 cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac
     6241 agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc
     6301 tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc
     6361 tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa acagtcgctc
     6421 ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg
     6481 cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc
     6541 caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag
     6601 acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata
     6661 aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc agccgaccag
     6721 catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct gcgccgccgt
     6781 gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata
     6841 ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc
     6901 ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt
     6961 gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata
     7021 aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg
     7081 ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta
     7141 cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa
     7201 aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat
     7261 tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg
     7321 gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt ccgccatgcc
     7381 gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct ggatgtcgcg
     7441 gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat tccctttgac
     7501 cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt
     7561 ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg
     7621 ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag
     7681 ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga
     7741 gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta
     7801 tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt
     7861 ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg
     7921 aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt
     7981 agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag
     8041 ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat
     8101 caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta
     8161 aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt aaactctatt
     8221 tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatac
     8281 catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta
     8341 aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta
     8401 ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac
     8461 tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg
     8521 ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt
     8581 caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt
     8641 caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc aggatcgtat
     8701 tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat
     8761 tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca
     8821 tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac aatcgtgctg
     8881 attcagaaac agggacaata tctccatagc cgacggttga catggtttct atcgaaaaat
     8941 agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac
     9001 cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg
     9061 cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc
     9121 aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt agcgctatca
     9181 acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt
     9241 tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca
     9301 actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata ccatagattg
     9361 ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg
     9421 tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg
     9481 taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg
     9541 tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagtgagc
     9601 aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata aagaaataca
     9661 gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag gaaaggccaa
     9721 acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAJTRW010000125         9757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRW010000125 DAJTRW010000000
VERSION     DAJTRW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            283..1401
                     /locus_tag="OO326_003495"
     CDS             283..1401
                     /locus_tag="OO326_003495"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5967752.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1467..1715
                     /locus_tag="OO326_003496"
     CDS             1467..1715
                     /locus_tag="OO326_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5967753.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1780..2148
                     /locus_tag="OO326_003497"
     CDS             1780..2148
                     /locus_tag="OO326_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5967754.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2242..2895
                     /gene="nfsB"
                     /locus_tag="OO326_003498"
     CDS             2242..2895
                     /gene="nfsB"
                     /locus_tag="OO326_003498"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5967755.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3003..4250
                     /gene="ybdG"
                     /locus_tag="OO326_003499"
     CDS             3003..4250
                     /gene="ybdG"
                     /locus_tag="OO326_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5967756.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4318..5694)
                     /gene="pheP"
                     /locus_tag="OO326_003500"
     CDS             complement(4318..5694)
                     /gene="pheP"
                     /locus_tag="OO326_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5967757.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5795..8938)
                     /gene="cusA"
                     /locus_tag="OO326_003501"
     CDS             complement(5795..8938)
                     /gene="cusA"
                     /locus_tag="OO326_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5967758.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(8950..9468)
                     /gene="cusB"
                     /locus_tag="OO326_003502"
                     /pseudo
     CDS             complement(8950..9468)
                     /gene="cusB"
                     /locus_tag="OO326_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2389 a         2696 c         2368 g         2304 t
ORIGIN      
        1 caaaatagct gtttatttat acagcaaatc ctgtttcgga cttctcactc cccatttgat
       61 caactgaagc tgaacccgtt tttttctgca tgcctcaatc gtcaaaattc ttcgccacca
      121 gccagctctg ccataacttg ccacattgaa aaagtagcca aagtgcttca ggcaccatca
      181 gttcaccatc accaaaaaaa agaaacattc cagaacttcc gcatctcgcc cagccgctcc
      241 gacttctata ctgaatagaa aacgtcaact taagagaaac ctatgccatt acccgatttt
      301 catgtttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt taatccgccg
      361 ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa taagatcacg
      421 gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac ggatgtttgc
      481 cgtgatatca accaggctgc cgggcagttt tcagcgatgc agaaagtcgt attgcaggca
      541 gccgcagacc atcatctgga aatttgcggc ggtggcacgc ccccgtttca gaaatggcag
      601 cgtcaggagg tatgcgacaa cgaacgctat caacgaacgc tggaaaactt tggctatctc
      661 atccagcagg cgaccgtttt tggtcagcat gtccatgttg gctgtgccag tggcgatgac
      721 gccatttatt tgctgcacgg cttgtcacgg tttgtgccgc actttattgc cctttccgcc
      781 gcgtcgccat atatgcaggg aacggatacg cgttttgccc cctcacgacc gaatattttt
      841 tccgcctttc ctgataatgg cccgatgccg tgggtcagta actggcaaca atttgaagcc
      901 ctgtttcgct gtctgagtta caccacgatg atcgacagca ttaaagatct gcactgggat
      961 attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac cccgttaacc
     1021 cttagccacg cggtaaatat ggcgggatta attcaggcca ccgcccactg gttactgacg
     1081 gaacgcccgt tcaaacatca ggagaaagat tacctgctgt ataaattcaa ccgtttccag
     1141 gcctgtcgct atggtctgga aggcgtcatt accgatccgc acactggcga tcgtcgaccg
     1201 ctaacggaag ataccttgcg attgctggaa aaaatcgccc cttcagcaca taaaattggc
     1261 gcatcgagcg caattgaagc actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag
     1321 ctgatgcgcg atttcgtcgc cgatggcggc tcgctgattg ggctggtgaa aaagcattgt
     1381 gagatctggg ccggtgacta agccgggaat tgccttacag aagaatatcc ctgacaatgg
     1441 tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg accaccgcag
     1501 caggcatttt gctgatggct ttcctctctt gcctgctgct gcccgccccc gcactggggc
     1561 tgacgctggc acaaaaactg gtgaccatgt tccatctgat ggatcttagc cagctttaca
     1621 ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt gttctgcgct
     1681 ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg
     1741 gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac
     1801 gaaacggcga aacgcctagc tcttgagtta ccctttgtcg agctttgctg gccttttggc
     1861 ccggagttcg atgtttttaa aattggcggc aagattttta tgctgtcgtc ggagctacgc
     1921 ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata
     1981 tacccaagca ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtattcc
     2041 ggcgaggaaa tctccgaagc gttacttcgc gatctgatca acgattcgtg gaatctggtg
     2101 gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta
     2161 tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa
     2221 ttacttacac atggagtctt tatggatatc atttctgtcg ccttaaagcg tcattccact
     2281 aaggcatttg atgccagcaa aaaacttacc ccggaacagg ccgagcagat caaaacgcta
     2341 ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt tgccagcacg
     2401 gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt caacaaacgt
     2461 aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc
     2521 tggctgaagc tggttgttga ccaggaagat gccgatggcc gctttgccac gccggaagcg
     2581 aaagccgcga acgataaagg tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat
     2641 gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaactt cctgctcggc
     2701 gtggcggcgc tgggtctgga cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat
     2761 gcagaatttg gtctgaaaga gaaaggctac accagtctgg tggttgttcc ggtaggtcat
     2821 cacagcgttg aagattttaa cgctacgctg ccgaaatctc gtctgccgca aaacaccacc
     2881 ttaaccgaag tgtaattctc tcttgccggg catctgcccg gctatttccc ctcagattct
     2941 cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat aaaggagacg
     3001 ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc gatcacacca
     3061 cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg catattattt
     3121 tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca cggctttggt
     3181 tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg caggggatta
     3241 tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca gatattctga
     3301 ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg
     3361 atgttatttt gaatctggcg cagaaattcc cggcggcatc tcagttaccg ctgaaaggga
     3421 tatttcaggg gattaaactg atcggcgcaa tcctggtcgg cattttgatg atctcgctgc
     3481 tgattggtca gtcgccagcg attctaatca gcggtcttgg tgcaatggct gccgtgctga
     3541 tgttggtatt taaagatccg attcttggtc tggtggcagg tattcagctt tccgcgaacg
     3601 atatgctgaa actgggcgac tggctggaaa tgccgaaata cggcgcggat ggcgcggtga
     3661 tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt accactattc
     3721 ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca gcatctggcg
     3781 ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt cttgatgaag
     3841 acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc agccgccatc
     3901 aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcggtatta aacctgcgcc
     3961 gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt aaccatccgc
     4021 ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat aacggtttac
     4081 cgctcgagat ctacgcgttt actaacaccg tggtgtggct ggaatatgaa agcattcagg
     4141 ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga cttcatcagt
     4201 cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac
     4261 cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt gaatgcctta
     4321 tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg gcagcaggat
     4381 cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc
     4441 gatgcaaaga tagttgccga acggatagag cagcgcctta aactgtgttt cacgcccctg
     4501 acgtcgcatc gccgcgcgaa aacgcagatg cgccaggcag atcataatcc agttcaacag
     4561 caacgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt
     4621 gattaacacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc
     4681 gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag
     4741 catgcggctg ttagagtaaa ccccgctgtt atacaccgac agcgatgcca ctagaatgac
     4801 gaagttcagc gcagaagcta ccacattgct gtcgagatta tggaaaatca tcacaaacgg
     4861 gctactgttg gatttcactt ccacccacgg atagagtgcc agtaaaacca ccagtgaacc
     4921 gatataaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa tgcttttttc
     4981 cggatcgcgc gcttcagcgg ctgtaatccc aatcagctcc agaccgccga aggagaacat
     5041 aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac caccgtagcg
     5101 ccagaggttg tcgatactgg ctttctcgcc gccgtaacca gaaaacagca gccacaggcc
     5161 aaagccgatc ataccgatga tcgccagcac tttaatcagc gcaaaccaga actcggtttc
     5221 gccatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc
     5281 ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag
     5341 ctctgccatt cccaccagca cgaacattac ccagtagttc cagccagaga ggaagcccgc
     5401 aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac
     5461 caccatctcg ccaagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc
     5521 cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa acagaccagt
     5581 accaattgcg ccacccagcg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag
     5641 cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt tcacgccttt
     5701 cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag
     5761 acacaatcca cacggttaaa cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca
     5821 gccacatcag cttatacgcc gccgggataa taaacagcga cagcaaaggt gcggtgatca
     5881 tgccgccaat catcggtgcg gcaatccggc tcatcacctc tgaaccagcc cccgttcccc
     5941 acagaatcgg cagcagaccg gcgataatca ccgccaccgt catcgctttc gggcgcacgc
     6001 gcaggaccgc gccgtgatac aacgcctcat ccagcttctg ctcgctgaat gtttgtggat
     6061 tattcaacga cggctcggcc tctatggcgt gacgtaaata catcagcatc accacgccaa
     6121 attcggcggc gaccccggca agggcaataa agccggtacc cgtcgccaca gaaagatgaa
     6181 agcccatcca ccacagtaac cagatgccgc ccaccagcgc aaacggtacg ctgctgataa
     6241 tcagcaacgc ttcgcccacc cgacggaatg ccagatacaa cagcacgaag atgatcatca
     6301 gtgtcatcgg caccatgagt ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc
     6361 cggagaatgc cacgctggtg cccggtttta actgcacttt ttcagctatc gctttttgca
     6421 aatcgtgaac caccgacacc atgtcacgat cgcgggcatc gatataaatc cagctcgtcg
     6481 ggcgcgcatt ctcggttttc agcatcgacg gtccggtaga gactttaacg tcggccacgt
     6541 ctgccagggt gatttgctgc ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg
     6601 gactatcgcg ccagctttgc ggataacgca gattaattgg ataacgggca atcccttcca
     6661 ccgtttcgcc aaccatcgcc ccgcccaccg cagaagtcac aaacaactgc acatccgcca
     6721 ccgtcatacc gtaacgtgcg gctttttcac ggttaatctc aacgttgata tagcgcccac
     6781 cttccagccg ctcagcaagc gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa
     6841 tttgctcagc catcgcgtcg atatccgcca gcacagtgcc ggaaacttta atgccgatgg
     6901 ggcttttaat gccggttgag agcatatcga tacggttacg aattggcggc acccacagat
     6961 tcgccagccc cggcagacgc acggtgttat ccagttcctc aatgattttg tccattgtca
     7021 tgcctggccg ccactgatcc tgtggcttaa gctggatggt cgtttctacc atctccagcg
     7081 gagcagaatc ggtggcggtt tccgctttcc cggttttgcc aaatacccgc gccacttcag
     7141 gtacgctcat aattagcttg tcggtttttt gcagcatact cgccgcctct gctgcggaaa
     7201 tccccggcag cgtcgatggc atatacaaca agtcgccttc attgatctgc ggtaaaaatt
     7261 ccccgccaac tttattgagc ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca
     7321 gcgtggtttt cggccagtgc agtactttca gcaacagcgg atgataaaca cgaatcaaaa
     7381 agcgattgag cggattactg ctttccggcg gaattttgcc acggatccag taacccatca
     7441 ggatagggat cactacgatc gccagcagcg ccgcacccgc catcgcatac gttttggtga
     7501 acgccaacgg gccaaacaga cgcccttcct gcccttccag ggtgaagatc gggataaacg
     7561 acaacgtgat aatcagcaga ctgataaaca gcgccggccc cacttcaaca gacgcatcgg
     7621 tgatcacctg ccagcgcgtt ttattatcca gcgtggcgtc aggatgctgg tgctgccact
     7681 cttccagctg tttatgcgca ttctcgatca tgacgatagc ggcatcgacc atcgccccga
     7741 cggcaatcgc aatgccaccc agcgacataa tattggcatt cagcccctgg aagggcatga
     7801 caataaaagc aatacacaac cccagcggca acgaaataat cgccaccagc gccgagcgca
     7861 catgccagag aaacggcgcg cagactaccg ccaccacaat aaactcttcc agcaacttgc
     7921 cgctgaggtt gtcgatggcc cggtcaatga gctggctgcg gtcgtatgtt gtaactatct
     7981 ccacgccttc cggcagacta cttttcagcg tttccagttt gtccttcacg gcggcgatca
     8041 cttctcgggc gtttttgccg gatcgcagga tcaccacccc gcccgccact tcgccttcgc
     8101 cgtttagttc ggcaatgccc cggcgcatct tcgggccaat ctggaccttc gcaacatcgc
     8161 gcagataaac gggcacgcca ttttcactgg cttttaaaac gatgtgatta aagtcgtcga
     8221 gcgtttgtag atagccgctg gcgcgcacca tatattctgc ttccgccagt tcgattgacg
     8281 aaccgcccgc ttcctggttt gaagcatcca gcgcgctttt tacttcggcg agactgatgc
     8341 catactgcgc caggcgctga ggatcgataa ccacctgata ctctttcacc acaccgccta
     8401 ccgacgccac ttccgcaacg tcaggaatgg ttttcagctc atatttgaga aaccagtcct
     8461 gcaatgagcg taaatcggca agatcgtgct taccgctgcg atccaccagt gcatattcat
     8521 agatccagcc aacacccgtg gcatctggtc ccagctcggc gctgactccc gcaggcagct
     8581 taccctgtac ctggttgagg tactccagca cccgcgaacg cgcccagtac ggatcggtgc
     8641 catcttcgaa aatgacatac acataagagt cgccaaactg cgagaaacca cgcacagtct
     8701 tcgcgccagg caccgacaac atggtggtgg ttagcggata agtcacctga ttttcaacga
     8761 tttgcggtgc ctggccggga tagctggttt taataatcac ctgcacatcg gagagatccg
     8821 gcaacgcatc cactggcgta ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg
     8881 cgcccatcag caccaaaaaa cggttcgcca ccgagcgacg aataatccat tcaatcattg
     8941 gttattccct caatgcgcat gggtagcact ttcagagcgc atccgctcca gtgcgccaga
     9001 aatattggct tcggaatcaa tcaggaacag gccgccggaa accacctttt caccttccgc
     9061 cagaccagag cgtaatgcgg tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg
     9121 tacaaagcgc ccgtcggcat caacggtaat cacccgctgt tcgttgccgg tatcaatcag
     9181 cgcctgtgac ggaatgagca gcatcggttc gctggcggtg ttgagttgca accaggcgtt
     9241 catgcccggt tttagcgcct cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg
     9301 ggtcgcggca tccacgccag gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg
     9361 tcgcgccgga acggtgaggg taaactgcga ggcatctttc accagccagg cgatggactc
     9421 cgggatcgca gcagtgaccc acaccgggtc cataccctga attcgcgatt acgcgcaatc
     9481 cccctgagaa ccgccatgaa caagggtttc atgatttgtg attttagttt ggtacgcaat
     9541 ttggtacaca acactatttt cacttcagcg ggtagtcatc aaactcacca taacgagcat
     9601 cgttgatgat gtatgtgatc accacaaata tcacatctgg actagttccg tcttcatgcg
     9661 caggtagact ggccccctga atctccagac aaccagtatc acttatttaa gtgatagtct
     9721 taatactagt ttttagactt gtcattggag aacagat
//
LOCUS       DAJTRW010000126         9679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRW010000126 DAJTRW010000000
VERSION     DAJTRW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(24..212)
                     /locus_tag="OO326_003503"
     CDS             complement(24..212)
                     /locus_tag="OO326_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5967759.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(431..1408)
                     /gene="epmA"
                     /locus_tag="OO326_003504"
     CDS             complement(431..1408)
                     /gene="epmA"
                     /locus_tag="OO326_003504"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5967760.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1733..3541
                     /gene="frdA"
                     /locus_tag="OO326_003505"
     CDS             1733..3541
                     /gene="frdA"
                     /locus_tag="OO326_003505"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5967761.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3534..4268
                     /gene="frdB"
                     /locus_tag="OO326_003506"
     CDS             3534..4268
                     /gene="frdB"
                     /locus_tag="OO326_003506"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5967762.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4279..4674
                     /gene="frdC"
                     /locus_tag="OO326_003507"
     CDS             4279..4674
                     /gene="frdC"
                     /locus_tag="OO326_003507"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5967763.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4685..5044
                     /gene="frdD"
                     /locus_tag="OO326_003508"
     CDS             4685..5044
                     /gene="frdD"
                     /locus_tag="OO326_003508"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5967764.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5107..6240
                     /locus_tag="OO326_003509"
     CDS             5107..6240
                     /locus_tag="OO326_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5967765.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6329..6862
                     /gene="blc"
                     /locus_tag="OO326_003510"
     CDS             6329..6862
                     /gene="blc"
                     /locus_tag="OO326_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5967766.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6859..7176)
                     /gene="sugE"
                     /locus_tag="OO326_003511"
     CDS             complement(6859..7176)
                     /gene="sugE"
                     /locus_tag="OO326_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5967767.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7352..7498)
                     /gene="ecnB"
                     /locus_tag="OO326_003512"
     CDS             complement(7352..7498)
                     /gene="ecnB"
                     /locus_tag="OO326_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5967768.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7609..7734)
                     /gene="ecnA"
                     /locus_tag="OO326_003513"
     CDS             complement(7609..7734)
                     /gene="ecnA"
                     /locus_tag="OO326_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5967769.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7786..8352)
                     /gene="efp"
                     /locus_tag="OO326_003514"
     CDS             complement(7786..8352)
                     /gene="efp"
                     /locus_tag="OO326_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5967770.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8394..9422
                     /gene="epmB"
                     /locus_tag="OO326_003515"
     CDS             8394..9422
                     /gene="epmB"
                     /locus_tag="OO326_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5967771.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2464 g         2270 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccattaa ggcgaatgga
       61 ataaagaata acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa
      121 taagccgatg ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct
      181 atcagactca tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta
      241 taacacgtgc ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac
      301 agaattgata cttggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa
      361 atgaaaacat atgctgcaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa
      421 tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc
      481 ccagcgccaa catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac
      541 cgactttcaa ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg
      601 cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat
      661 ggaaaccatt cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat
      721 gatcttcggt actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg
      781 tcggtttttc tttaccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg
      841 tgtcgcggtc ttcttcggta tcagcaacat tgctcaaatc cagtttcgct gcgacttccc
      901 gcagttgcgt tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt
      961 gataagaaag gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct
     1021 cgttcatcaa ccggtacata tcatagtgcg gtcgatacca ctccagcata gtgaactcag
     1081 ggttgtgata acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc
     1141 cacaaccagc aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat
     1201 tcatcccctg cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat
     1261 cggttaccgt cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcggcaa
     1321 agaaacgacg gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc
     1381 tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga
     1441 agtctactcg caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca
     1501 gtgactaaaa aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa
     1561 tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga
     1621 ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg
     1681 cacttaccct gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac
     1741 ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc
     1801 tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg
     1861 tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt
     1921 cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt
     1981 ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt ggggatgccc
     2041 atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga
     2101 gcgcacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac
     2161 ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt
     2221 tgatgatggt catgttcgcg gcctggtagc aataaacatg atggaaggca cgttggtgca
     2281 gatccgtgct aacgcggtcg ttatggctac cggcggtgcg ggtcgcgttt atcgttacaa
     2341 caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc
     2401 gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct
     2461 gatgaccgaa ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta
     2521 tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat
     2581 ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa
     2641 caccatctcc acgccgcgtg gtgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa
     2701 aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga
     2761 tccggttaaa gaaccgattc cggtacgtcc gactgcacac tacaccatgg gcggtatcga
     2821 aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc
     2881 tgttggtctg cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt
     2941 cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga
     3001 agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tagttaacca
     3061 ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg
     3121 ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct
     3181 gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct
     3241 gctctacacc attgaactga gccacggtct gaacgttgct gaatgtatgg cgcactccgc
     3301 aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg
     3361 tgacgacgtc aactttctca aacacaccct cgccttccgc gatgctgatg gcacgactcg
     3421 cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg
     3481 cgaagcggat gcagccgata aggcggaagc agccaataat aaggagaagg cgaatggctg
     3541 agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc
     3601 atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct
     3661 acatcaaaga caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt
     3721 gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc
     3781 gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc
     3841 tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg
     3901 gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt
     3961 atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg
     4021 gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag
     4081 atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggtgtat
     4141 ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg
     4201 cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac
     4261 cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc
     4321 acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt
     4381 ccggctgtgt ggttcagcat tgaactgatt ttcgcgctgt ttgccctgaa aaatggcccg
     4441 gaagcctggg cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg
     4501 atcactctga cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg
     4561 gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg
     4621 gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc
     4681 tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg
     4741 ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct
     4801 gctgccactg gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc
     4861 gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg
     4921 tttacaccgt atgcaccacg cgatgcacga tctgaaaatc cacgtgcctg cgggcaaatg
     4981 ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggtg tcgttacaat
     5041 ctaacgcatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca
     5101 gaccttatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctctta ctccacattt
     5161 gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa
     5221 caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc
     5281 tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta
     5341 ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc tcgaggggaa
     5401 atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat
     5461 gggatcacac tattacatct tgcgacctac accgctggcg gcctgccatt gcaggtgccg
     5521 gatgaagtga aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg
     5581 gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct
     5641 gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc
     5701 aaactcaacc atacgtggat taatgtacct cccccagaag aaaagaatta cgcctgggga
     5761 tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga agcttatggt
     5821 gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa gcaatttacc cccccttgat
     5881 atcaatgaga aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc
     5941 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc
     6001 atcattaacg gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag
     6061 cccccaactc ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt
     6121 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa
     6181 aactatccca atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa
     6241 aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac
     6301 ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
     6361 agctgcattt ctggtcgttg cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt
     6421 gaataatttc gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca
     6481 ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg
     6541 cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg
     6601 gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc
     6661 tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg
     6721 cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt
     6781 gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg
     6841 ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat
     6901 taacgccaga ctcgccagac gcatcgggtt agcggactca ccgagcagca caatgccggt
     6961 gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc ctaccggtaa
     7021 cgatttcatc gcccaggcaa gtagcgccat actgactatc atcgccgtca cggtaataac
     7081 actcggcgtc aaacggctaa agccgtgggt atatttcagg ccaacggccc ataccacttc
     7141 cagcagacca gcaataacta agataatcca ggacatatca ggctccggaa atttggggcc
     7201 gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat
     7261 tttgccaggt ggcaggggaa tttcaactat tacctttatg acgatcgcag aaaaaacaaa
     7321 cggcacgaca ctgctgtcgt accgttattg cttattgctg cgctttcgtt gctgcgccag
     7381 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
     7441 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
     7501 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
     7561 tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
     7621 gcgagagatg gagttaccga gatgtttgat gtcttcgccg aaaccgcaag cggtgttaca
     7681 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
     7741 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
     7801 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
     7861 aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
     7921 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
     7981 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
     8041 agcattgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
     8101 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
     8161 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
     8221 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
     8281 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
     8341 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
     8401 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
     8461 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
     8521 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
     8581 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
     8641 ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc gtagtgcctg
     8701 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
     8761 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
     8821 actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag atgattttct
     8881 ccggcggcga tccgctgatg gcgaaagagc acgagctgga ctggttgctc acacaactgg
     8941 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
     9001 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
     9061 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
     9121 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
     9181 acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt
     9241 acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
     9301 cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg ccgaaactgg
     9361 cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
     9421 aactgattaa acagggaagt taaacattaa aattgcctga tgcgctacgc ttatcaggcc
     9481 tacatcgtgc attgaaattg aatggatttg caatgaactg taggccggat aaggcgttta
     9541 cgccgcatcc ggcaagagca acaaacgcaa gtacagcaac cactgagaaa tttaatcttt
     9601 gcacgttgca taacctttaa ttattcatat gcattgctgt tgttactttt tatatagcta
     9661 acaccaataa ttaaccttt
//
LOCUS       DAJTRW010000127         9596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRW010000127 DAJTRW010000000
VERSION     DAJTRW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO326_003516"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO326_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5967772.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO326_003517"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO326_003517"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5967773.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO326_003518"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO326_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5967774.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OO326_003519"
     CDS             complement(2404..2607)
                     /locus_tag="OO326_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967775.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OO326_003520"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OO326_003520"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5967776.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OO326_003521"
     CDS             complement(3803..4015)
                     /locus_tag="OO326_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5967777.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OO326_003522"
     CDS             4275..4583
                     /locus_tag="OO326_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5967778.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO326_003523"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO326_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5967779.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGSVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO326_003524"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO326_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5967780.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OO326_003525"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OO326_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5967781.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OO326_003526"
     CDS             complement(8479..8982)
                     /locus_tag="OO326_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5967782.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9596)
                     /locus_tag="OO326_003527"
     CDS             complement(9070..>9596)
                     /locus_tag="OO326_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5967783.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2449 c         2285 g         2480 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgaaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagc
//
LOCUS       DAJTRW010000128         9501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRW010000128 DAJTRW010000000
VERSION     DAJTRW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..581)
                     /locus_tag="OO326_003528"
     CDS             complement(<1..581)
                     /locus_tag="OO326_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5967784.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSG"
     gene            complement(648..1373)
                     /gene="fimC"
                     /locus_tag="OO326_003529"
     CDS             complement(648..1373)
                     /gene="fimC"
                     /locus_tag="OO326_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5967785.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1410..1949)
                     /locus_tag="OO326_003530"
     CDS             complement(1410..1949)
                     /locus_tag="OO326_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5967786.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2014..2562)
                     /gene="fimA"
                     /locus_tag="OO326_003531"
     CDS             complement(2014..2562)
                     /gene="fimA"
                     /locus_tag="OO326_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5967787.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3043..3639)
                     /gene="fimE"
                     /locus_tag="OO326_003532"
     CDS             complement(3043..3639)
                     /gene="fimE"
                     /locus_tag="OO326_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5967788.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4117..4719)
                     /gene="fimB"
                     /locus_tag="OO326_003533"
                     /pseudo
     CDS             complement(4117..4719)
                     /gene="fimB"
                     /locus_tag="OO326_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6173..6889
                     /gene="nanC"
                     /locus_tag="OO326_003534"
     CDS             6173..6889
                     /gene="nanC"
                     /locus_tag="OO326_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5967789.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6909..8015
                     /gene="nanM"
                     /locus_tag="OO326_003535"
     CDS             6909..8015
                     /gene="nanM"
                     /locus_tag="OO326_003535"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5967790.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8080..9060
                     /locus_tag="OO326_003536"
     CDS             8080..9060
                     /locus_tag="OO326_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5967791.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2782 a         2072 c         1993 g         2654 t
ORIGIN      
        1 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
       61 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
      121 tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa tggcacacag
      181 gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc catactggcg
      241 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
      301 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
      361 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
      421 aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc ggcaaaagca
      481 caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
      541 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
      601 cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta ttccattacg
      661 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
      721 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
      781 agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt cgggttaatc
      841 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
      901 aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc gagctgtagc
      961 atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt catccagaat
     1021 aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg taaggtattc
     1081 tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca
     1141 ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc attatttgtc
     1201 acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc
     1261 actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
     1321 aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact cactgtcctg
     1381 ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa ccaggcctgg
     1441 gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc gatgaaatgt
     1501 agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt aatcggtacg
     1561 aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc tggcccctct
     1621 cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
     1681 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
     1741 tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
     1801 ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
     1861 ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg cagcaacgac
     1921 gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
     1981 ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct tgaaggttgc
     2041 atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg cctggaacgg
     2101 aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac cgtccagcgt
     2161 cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac tacctgcagc
     2221 agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac ctaagaaggc
     2281 aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt taaaaccaac
     2341 ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc ccaactgaac
     2401 ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
     2461 gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg aactgaggga
     2521 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa
     2581 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
     2641 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
     2701 tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat attctattat
     2761 tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa
     2821 gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt gaaattaatt
     2881 tacaagagaa ataatttaca tatcaaatag ttagatgctt tttgtcgttt tttaatattt
     2941 ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagtttggc cccaattgtc
     3001 ttgtgtttat ttgtttttta actttattat caataagtta aatcaaacct cttctctttt
     3061 taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact
     3121 ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg
     3181 ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg
     3241 agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat aggcctgctg
     3301 gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt cagcgccttt
     3361 ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa aacgtaacgg
     3421 gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc
     3481 aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa
     3541 cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc
     3601 ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga
     3661 gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata
     3721 aaaagataac cacacacgaa gttatgtggc tatcagtcaa ttacattaaa catcatacaa
     3781 aaataaaata tcactgatgt gacaacatct attttcattg atttgattca atataacaat
     3841 agattacaga ggattaactt ccagttactt aaaaaataaa gaaaaaagtg tggaaacagg
     3901 agaatttaag tgatgtgtaa acttatgaat gattttgttt atttattttg aagaacataa
     3961 atcattagat tttgtatggg tatggttaac ttaatgttta ttatgataat taagaatcac
     4021 acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt aagaataatg
     4081 tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat gacgctgtcg
     4141 tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg tataccagac
     4201 agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat ctattcccat
     4261 attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct ccagtgacaa
     4321 cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag aaagcggatt
     4381 ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag tacggatact
     4441 caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg ttgaaaagcc
     4501 tttttttaat cgatggatat atatacactt tgctttaaga tcaatatccg aaatcctcaa
     4561 tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca gacaataatt
     4621 acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt cactatgggt
     4681 aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt ataattacag
     4741 acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga ttatgtttat
     4801 aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga gcgaacttaa
     4861 atggagtagc gatagttact tataataact ttgccataca aacgccatgc tcttgcatgc
     4921 tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct catcattaat
     4981 gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa tcaacataaa
     5041 aaattcagca aaatttaata tttgttaaac tagccgtctc aaatatgaca ttattagtta
     5101 actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag tggctattat
     5161 catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct taggccatga
     5221 gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg ctttttcatc
     5281 tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc aatcacagct
     5341 attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa caccaacatc
     5401 aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa ccaagaaaca
     5461 tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt ttcaccactc
     5521 tcactgagtt agcttgcaaa gctttctttg tttagattct aaataaatta tttataaaac
     5581 gtatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg tctggcttca
     5641 catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt gcaaatctgg
     5701 ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag atgcaccacg
     5761 atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag ttctttcaca
     5821 tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc aacagacact
     5881 ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg aatattcaaa
     5941 tcaattaaaa atcagaagag attttatact caggtattta atctggatct ctgtttattt
     6001 aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata aaatacagtt
     6061 acccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat
     6121 tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa
     6181 ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa tttctcaggc
     6241 tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact
     6301 caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa
     6361 taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa
     6421 ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag
     6481 cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct
     6541 taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg
     6601 tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag
     6661 tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt atcgctatgc
     6721 aaaccacaag aaatgggcga cggaaaatgc ctttgttcta caataccata tgacgcccga
     6781 tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca acggcagaga
     6841 taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga
     6901 cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca tttgccgcaa
     6961 acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga gcaattgata
     7021 acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag ctagatacac
     7081 aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg agagagcaag
     7141 caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg
     7201 agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagctggg
     7261 ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt gtacacaacg
     7321 gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat tttgaagatc
     7381 tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac tattttgaca
     7441 aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc tcaacacagc
     7501 aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg gttgtgaata
     7561 aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga acggatgccg
     7621 tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat cccgtctcat
     7681 caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt atatttgctg
     7741 gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac tatgcgcatg
     7801 aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata
     7861 aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtt
     7921 tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttaattt
     7981 ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg gtaaaggggc
     8041 cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat aatatcaccc
     8101 gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta tggcgaagga
     8161 ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt agcgagattt
     8221 gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc actgactcac
     8281 tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac gaatcatcaa
     8341 acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact gcccttcatt
     8401 cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc ttttaccgcg
     8461 ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga
     8521 acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact ggcaaaaaat
     8581 ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt aatgaccagt
     8641 gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg tagggatcta
     8701 aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc
     8761 acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg caattatcaa
     8821 aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga aagaggactg
     8881 acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg ttcagcgtac
     8941 cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac ggcagcccgt
     9001 cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg cgaaaggtga
     9061 gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca acaaaaaaca
     9121 aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa ttgccaatat
     9181 aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat tgcttacaga
     9241 ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga aatctacgaa
     9301 catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg tcctgatatg
     9361 ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga tatgcttaat
     9421 gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt cattcctaaa
     9481 agcacccgcc attaatccca g
//
LOCUS       DAJTRW010000129         9422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRW010000129 DAJTRW010000000
VERSION     DAJTRW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            29..1144
                     /locus_tag="OO326_003537"
     CDS             29..1144
                     /locus_tag="OO326_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967792.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1823..2041
                     /locus_tag="OO326_003538"
     CDS             1823..2041
                     /locus_tag="OO326_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5967793.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2259..2501
                     /locus_tag="OO326_003539"
     CDS             2259..2501
                     /locus_tag="OO326_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967794.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2831..3760)
                     /gene="fdhE"
                     /locus_tag="OO326_003540"
     CDS             complement(2831..3760)
                     /gene="fdhE"
                     /locus_tag="OO326_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5967795.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3757..4392)
                     /gene="fdoI"
                     /locus_tag="OO326_003541"
     CDS             complement(3757..4392)
                     /gene="fdoI"
                     /locus_tag="OO326_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5967796.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4389..5291)
                     /gene="fdoH"
                     /locus_tag="OO326_003542"
     CDS             complement(4389..5291)
                     /gene="fdoH"
                     /locus_tag="OO326_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5967797.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5304..8354)
                     /gene="fdnG"
                     /locus_tag="OO326_003543"
     CDS             complement(5304..8354)
                     /gene="fdnG"
                     /locus_tag="OO326_003543"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7767..7769),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5967798.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8548..9381
                     /gene="fdhD"
                     /locus_tag="OO326_003544"
     CDS             8548..9381
                     /gene="fdhD"
                     /locus_tag="OO326_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5967799.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2307 a         2313 c         2443 g         2359 t
ORIGIN      
        1 gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa cagctgataa
       61 tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga tcaccaataa
      121 atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg tatatcatgc
      181 ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg tgttatgtgc
      241 tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag gaaatgtgcc
      301 aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg caattaagca
      361 gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa tgatgaggga
      421 ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg aagcgcatag
      481 ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga caggtgtggg
      541 cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc tttctggttc
      601 gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt cggatatttc
      661 tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag ataaattaaa
      721 tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga cggcgataaa
      781 tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat ctgcgaaaga
      841 tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg aaaaacgcct
      901 cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta gttgcgattc
      961 attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag ttcttcatgt
     1021 ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt tacaagattc
     1081 gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag ggatgggagt
     1141 ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca aaaatgacat
     1201 acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc atacagaaag
     1261 taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag gagttttgct
     1321 atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa actcatcgtc
     1381 gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg aaggaattcc
     1441 ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga ccggttggtg
     1501 gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg tgctgttagg
     1561 atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc tgaatataat
     1621 atattcatag cacccataac tcatcctggg cgataactaa accatttttg agtaataaat
     1681 gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca aaatgacatg
     1741 caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata cattaatgag
     1801 taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt tatcagaaga
     1861 ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat ctgcaatttt
     1921 acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta aagcaaaggg
     1981 aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc ataaggaatg
     2041 aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt attaatacaa
     2101 ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg aaaacgcctc
     2161 gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa gggtactttt
     2221 ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg gcaggtatag
     2281 atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt gatgatctcg
     2341 aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat caatacctga
     2401 taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg tgtgaggaag
     2461 atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa atgatgtcaa
     2521 tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg ccttatccgg
     2581 cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga taagcgcagc
     2641 gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc gatgtgttgg
     2701 agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa cgcggaatac
     2761 gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc cacccggaaa
     2821 acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa ctacgggcgt
     2881 agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct gccacggctt
     2941 caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag tcatcgcagc
     3001 tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct
     3061 gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac aggttgcagt
     3121 gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc ggcatagagc
     3181 cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg ccggggatca
     3241 gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc gctttatcgc
     3301 tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc agctcctgag
     3361 tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt tcaggtttca
     3421 gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc agaacgtgaa
     3481 tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc agatccatct
     3541 ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca gcaaagcgca
     3601 ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg cggcggttgt
     3661 ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta cgtttctcgc
     3721 tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct tttccgttgt
     3781 cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc tggtaaccca
     3841 tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat
     3901 gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga atcggatcac
     3961 cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc tcaccagcag
     4021 cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt tataacgccc
     4081 ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc aaaagatatc
     4141 atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga acgaggcaaa
     4201 cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc ccatgatttg
     4261 catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa
     4321 gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc
     4381 acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc ttcctcatcc
     4441 gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt agccgcaaag
     4501 ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc gctgatttcc
     4561 gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag cacgtacatg
     4621 acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta accgcgggtt
     4681 ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt gccgaagtga
     4741 atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac cacgcggtca
     4801 acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg aatgtcgaac
     4861 ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa gtcgacgata
     4921 ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca gcctggatcg
     4981 gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt ctgctccact
     5041 tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt gtcgtacacc
     5101 ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc cacctgacag
     5161 gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc ctggaagtcc
     5221 cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat atcttgagat
     5281 tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa ggacttaaac
     5341 tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc aataaagcct
     5401 tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc gatatctttg
     5461 ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt gatatagcca
     5521 cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt cgccagcgac
     5581 tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc gtgtttggtc
     5641 cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa cttatcggct
     5701 ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga gataacgttt
     5761 ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga
     5821 ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg catgataaac
     5881 ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca gccggtccac
     5941 ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc ctgcggatct
     6001 gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc
     6061 gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt gccttccggc
     6121 gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc gcgcaattgg
     6181 gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc cggatcggta
     6241 atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt cggctcatgc
     6301 ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc accctgttcg
     6361 gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc agtcagcgca
     6421 atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga gttaacgatt
     6481 gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt ctggatcttc
     6541 gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt ttcagtgttc
     6601 agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac tttgtttttg
     6661 ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac cttgccctct
     6721 ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg caaccagtca
     6781 aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat catagagacg
     6841 aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg ttttggcgtg
     6901 ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt catgtaacct
     6961 ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga gtgaccgcgc
     7021 agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg gatcatcgcc
     7081 atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc atacaggaac
     7141 gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt caggaacgcg
     7201 tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga aacgtgctgt
     7261 ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt ggcgaagccg
     7321 ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc cgcgtcgtag
     7381 ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct ggcgttggta
     7441 taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag caatacgcct
     7501 gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc taccgccgcc
     7561 gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat tttggcttcc
     7621 atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat caccacgacg
     7681 aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     7741 gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata
     7801 cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga cgcggaagca
     7861 cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt ttgcgcaatg
     7921 tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa
     7981 ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt cagacggctt
     8041 tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc gcggttgacc
     8101 gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt tgctccgtca
     8161 ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt attacgggtt
     8221 tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct gggtgcaaaa
     8281 cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa gaactgcctt
     8341 ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt tacattcgct
     8401 tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc aaaggtccag
     8461 aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat aatgttggtg
     8521 tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga aatcgaaaat
     8581 gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga tttacaacac
     8641 ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa cggcatttcg
     8701 catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg tttttcgctt
     8761 tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt tccttcctgt
     8821 aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt gaaggagcgc
     8881 cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca acttaatgac
     8941 atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa caaactggat
     9001 gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg ttgtactcac
     9061 gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc
     9121 catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag ctggcagcaa
     9181 ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgc
     9241 ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga agtggctgag
     9301 cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt ttatacccat
     9361 ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa ttgcaggact
     9421 ta
//
LOCUS       DAJTRW010000130         9418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRW010000130 DAJTRW010000000
VERSION     DAJTRW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(226..1128)
                     /locus_tag="OO326_003545"
                     /pseudo
     CDS             complement(226..1128)
                     /locus_tag="OO326_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1188..1466
                     /locus_tag="OO326_003546"
     CDS             1188..1466
                     /locus_tag="OO326_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967800.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1467..1994)
                     /gene="gadE"
                     /locus_tag="OO326_003547"
     CDS             complement(1467..1994)
                     /gene="gadE"
                     /locus_tag="OO326_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5967801.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2793..3365)
                     /gene="hdeD"
                     /locus_tag="OO326_003548"
     CDS             complement(2793..3365)
                     /gene="hdeD"
                     /locus_tag="OO326_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5967802.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3620..3952
                     /gene="hdeA"
                     /locus_tag="OO326_003549"
     CDS             3620..3952
                     /gene="hdeA"
                     /locus_tag="OO326_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5967803.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4068..4394
                     /gene="hdeB"
                     /locus_tag="OO326_003550"
     CDS             4068..4394
                     /gene="hdeB"
                     /locus_tag="OO326_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5967804.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4458..5104
                     /locus_tag="OO326_003551"
                     /pseudo
     CDS             4458..5104
                     /locus_tag="OO326_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5146..5676)
                     /locus_tag="OO326_003552"
     CDS             complement(5146..5676)
                     /locus_tag="OO326_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5967805.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5832..6398)
                     /gene="slp"
                     /locus_tag="OO326_003553"
     CDS             complement(5832..6398)
                     /gene="slp"
                     /locus_tag="OO326_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5967806.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6645..7868)
                     /locus_tag="OO326_003554"
     CDS             complement(6645..7868)
                     /locus_tag="OO326_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967807.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8449..>9418)
                     /locus_tag="OO326_003555"
     CDS             complement(8449..>9418)
                     /locus_tag="OO326_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5967808.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2748 a         1872 c         1956 g         2842 t
ORIGIN      
        1 cctttaaaat tcgtttttaa aggctacgat gacatttttg aagagtctta aaagaattaa
       61 aatacacggc gcgcctatct tgaatgtttt atcgatatcg aagcattgag catttttaac
      121 tcgttttatc ttgaatgcaa tcggcgcata acttattgaa tcccatcaaa tcccgtcttt
      181 ttttacccat tcccggttat cttgaataaa ttacctagtc ataaatacat tctggcggct
      241 accttcatcc actaatgccg tgacatacat gccaggcagc aagtcaccat ttgggttggg
      301 gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatca accgtcgggt cggagaattt
      361 cagcgtgccg gtctggctgt aacgtttacc attttccaga ttgagctgta ctggcgtact
      421 gccctgaacc tgtttgattt gcccactggc gacctcttct ttcatgcgta agaaatcttg
      481 caccgactgc gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc
      541 tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt
      601 aatcggcgag gtgacattcg cgtattgcag attgatcgtc gcctgttcaa cagccgcttt
      661 ggcgacggtg acattggctt ctgcttcatt cagctgggtg cgcgcggtgt cgtaatcctg
      721 acggctaacg tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt
      781 gctggcggta gagagcgctt tcgccagcga gcctttggcg gagtttagct cggcctgtaa
      841 aggtgcagga tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa
      901 gttgcgttta atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac
      961 ggttctaccg ggcaattcgc tcaacacatt gaccgaacca ggggagagtg tgacgacacc
     1021 gacctcaggc gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat
     1081 cgcgccgcag aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga
     1141 aaattcttga gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac
     1201 agaccagcgg catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac
     1261 atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc
     1321 gggcaacgaa gggttctact ggtggataca cataccaggg gaatcaagtc gttttagcct
     1381 ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc
     1441 atcaatttca gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg
     1501 tcgctcatac gtcttagttt cagcttgtac ataatgttga atttctgaat ctttaatgtt
     1561 tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt
     1621 tgcataattt tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt
     1681 ttaccataag taatctcaag aagatttttg atactttctt tgcggctaac aaaataaata
     1741 ggcgctttag cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca
     1801 tctaatttct ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca
     1861 atgataacct tgaagggttt attgcctact tttttgatct ctgacaggga attgattttt
     1921 atcatttcgt gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc
     1981 atgagaaaaa tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca
     2041 ttatttacat ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc
     2101 cttcgtgaac taatacgtct attaacacta atcaatactt cagatttatc catcacgcac
     2161 tccatatatt gatcctaagt aagattcctg gttgttatca gcttgtaaat agtaaacaaa
     2221 aagttaacgt ttgcggatta ctgcccaaga ataagttatc aataaccagt ttgtgatctc
     2281 tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg
     2341 acttaagctg gcaattggat tgccagcttt cttttaacgc attgaaatta cctgagtaaa
     2401 taacgcattc atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat
     2461 gtaatcaacg ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat
     2521 caacgcttac ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac
     2581 tacggaaaat atcagccatg ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa
     2641 attagatgaa attcagtagg ttgaaataat cactagcagg taattatttc aatgatagtg
     2701 cgcaattgat ctacaacact gcgtagcgga gagagtatta atcggatcat agtcacatca
     2761 agtgactatg atccgggtga caaccggggt aattattgct gcttaacgaa caaactggcg
     2821 aagctgaaca ggctggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc
     2881 gttacagaca ccatcggcgt tgcaccaagg aatatccagg caatgacgat atccagcaca
     2941 ccaataacga gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg
     3001 cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc
     3061 agctccggtg cacggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa
     3121 ccggataata ccggccagaa attatgactg cggttgctga ataacccgac aataagcgca
     3181 atacccgagc agattaataa tgcacccact actgtgctta aaatatcgcc agagacgaac
     3241 gggaaactga tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct
     3301 ctgcgatgct ttttcagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat
     3361 aacatagaac caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa
     3421 acgcgtctaa gaatgcagtc gatttaataa aaatttccta attgcagtat ctgatgcatc
     3481 tgtaactcat tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat
     3541 atacagaaaa ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt
     3601 tatagttgag gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct
     3661 tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca gctgataaca aaaaaccggt
     3721 caactcatgg acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt
     3781 tggttttgct gaagcgctga acaacaaaga taaaccagaa gatgcggttt tagatgttca
     3841 gggtattgca accgtaaccc cagctatcgt tcaggcttgt actcaggata aacaagccaa
     3901 ctttaaagat aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga
     3961 atgcgttaca tcgtacttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag
     4021 ctattcctcc tgttcatata taatctctat attgaatggg ttacaaaatg aatatttcat
     4081 ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac
     4141 aatctgcgtt ggcagccaat gaatctgcta aagatatgac ctgccaggaa tttattgatc
     4201 tgaatccaaa agcaatgacc ccggttgcat ggtggatgct gcatgaagaa acagtatata
     4261 aaggtggcga taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg
     4321 aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg
     4381 acttgccgaa ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct
     4441 cctttttgca tttttatatg acagcagaat ttattatacg tcttatactc gcggcaattg
     4501 cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac
     4561 atgtattaat tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg
     4621 acgtgctgtc tttagatcac gtcggactcg accccagccg tattgctgct caggtggtga
     4681 cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc
     4741 tgacgacggc agcggatatc tgggtgaccg ccgcataggt atggttattg gcagcggtat
     4801 gtacgaactg ggtatttatg gttcagtgat gaccttgttg gtgctggaag tcttccatca
     4861 attaaccttc cgcctgatga acaaaaatta ccatttacag ctgacattag tgaacggtaa
     4921 tacggtatca atgctcgact ggtttaagca gcaaaaaata aaaactgatt tagtttcgtt
     4981 gcaggaaaat gaagatcatg aagtggtcgc cattgatatc cagcttcatg ccacaacgtt
     5041 gatagaagac ctgttgcgat tattaaaagg tatggcaggg gtgaaaggcg tttcaattag
     5101 ctaatctgtg tggcagcgta gccagactca ccgtaagcct gaaattcaca ccagataatc
     5161 aatatgctga tggcgtacca gctcattgat ccgcttaaga ttgaggatca tcatgatatt
     5221 gtgtttatgg cagtagaacg tttttagcga tattttcagt ttctccagaa tatcttcagt
     5281 tgtcatttga ttcgcaatgc agctcagaac ctgatgttgc ttgcgcgaaa gtgacacatt
     5341 cgcttcaggc acagagcagt aactgccatt gatgagtgcg acaaaatcaa tgatagtgat
     5401 agtccgcgga acaaaggtca ccggtacttt ccccagacag cgtttaatat taaaggggga
     5461 gaaaacaatg acatggacag gttttaatcg ttcaatttga gtgagaaaat tagaatgaaa
     5521 tacattattc attaatgtat caataatgac gaaggcattc tgatgcaaca ttacgtcgat
     5581 ctcttcttcg ttctgtatat gaacgacatt acctttactc agaatgtttt tcatcgcggt
     5641 gaagaacatc gtatccctgg taattataag aaacataacc gactcctggt gcggacatta
     5701 actttataat cagtttgcca aaggtaatta acaggacagg gcttataata ataatagtta
     5761 gcttactgta tattaatttt atgtaataca tgatgcagat gctgagaaag catctgcatc
     5821 tttcggtggt gttatttgac cagctcaggt gttacctgac tcaccgcatt ggtgtagtaa
     5881 ggcgcacccc agcccggttc cggccagaat gcaccatagc cgtaatccca caggttatag
     5941 gtggtattaa ccacttctct caaatgccac acctggatgc cctgcatatt cacttccagg
     6001 aagttatacg ggactttatt gataaagcct ggttgttcac cctgaatggt accgaggatg
     6061 gtaacaaagt gattacgata gttcactgga tcaaggaagc cgctttgtct ggcgagcagt
     6121 cggccctgat agttggcttc aatatcaggc ttcgcatagc tatccaacgg taatacagcg
     6181 atttctaaca acgtatccgt tttgccattg ataacgttga taaccttccc accaaagcgc
     6241 gcttgttgac caacatataa ccccggctgg ttatgaacag caacaaaact tttttgaata
     6301 tcaggttggt tattgccttt gatattttgc ggaattgaac tacatgcggc aagcaaaaat
     6361 gaaaggctga ggatgagtgc accttttgtc atgttcatgt tactatcctt atcaacaaat
     6421 attaaaacca taatagatat ttctttatag ttttcatctg attctgaggt gatatgcctg
     6481 atagtaacgt aaacctgtct gatgtttata tcccttctgt ttagttcaat tccgttattt
     6541 tggggcgtgg tatgagtatc catcgcccaa taatataacc ctttgtttta aaattacata
     6601 aaatctattt ttattcaatg attataaagt gctaataatc aatattatgg tattatattc
     6661 tgataatgat ttgataagcg aattcttttg ccagatggta tttctatttc caccccataa
     6721 tttatatctt tttcattagg cagatctctc tttaaataaa aggggtatga aatcttttcg
     6781 ggttgaagtg tctcgtaatg aaattctatg aattcatttt tagacattgt tgcccatttt
     6841 tgaattttat tgtatagaga gggtgttgta aactcttgaa gttgtttata acatattttt
     6901 ttgaactctt gcttatattt taatatgtca tcaaatgaaa gattcactac ctttgcctct
     6961 aagaaatcaa ttattctcga agcaatttcc gctttcgcgc gtaaagagtt gtcattaata
     7021 gttaacaatg tagaaaacaa accattgatt tgtttgtcaa gatgttgttc atatttatgt
     7081 agtttgggga tgggattttc cttgtgtgaa aaaatagtgt taaataaatt tgcgtcagtg
     7141 aaatttatat taagtagatc ttcaaaataa aattttgata aggttaggac tagcttggac
     7201 atatcttgcc ctgtcatgtt gaaatcaata aagtcgcatc cagcaagggc ttccatccta
     7261 ttttcatttt tactgtgctc aatatacagc ttcgccattt ttaagtacaa gctttgaatg
     7321 tctcttatat cagttggtaa gtcagctcgt tgcccagcaa aatttttatt atcggaagaa
     7381 acggtttgga cactgaatct ttcgggtatt ccttcttcta cgagaatttt agttatgatt
     7441 ggattgttgg gttctggatt aacgacaaca atgatttgac atacagaatc cttatccttt
     7501 tcgtatctaa tgttatgaat ctgattaagt attgattgtg tctgttgcgt aagtggtgtc
     7561 gaacttatcg tttcaatgtt attaataaga cttacaagtt gaaccacaac ttttgagtag
     7621 tctgcatttg tatatgtggt gttcgtgtgg catgcattaa tttgttggag ttgttgttgc
     7681 gctaaatcaa attgttcagg gttgtatcct tctccagaga atgtggaatt aatgggttgt
     7741 tgttcttccc aattatcaca aggccacttc ggtgtgtctg gcgttttaac attatcggta
     7801 tttgttgtgc agttataaat atcgccgtga tatgtattta ttatacctgg ggtaaagtca
     7861 attgacataa cattccttat tcatcatctg ttttatgcta atgtttttaa attggataat
     7921 tagaacagga atggattttc ttctacacac aataaatata atgcgttata tcttcctgat
     7981 ataattacct ttaaattccc tttcatttcc acccggtaat atttgtgtat aaaaaaaggg
     8041 cagtgatagt atcgtaaagg aaaacgttaa caactatcaa aaaacgctca ttttaaaaag
     8101 ttattatgtt ctccgatagt gtgtgaagta atatttttgt gatgttttag atgtgtaata
     8161 atgaaccagg ttaaagtgta tttaaaaatg tgttgtatta gcgactgaaa tctgcctttt
     8221 aaaaaggagg agggtactga gacaaagagt cattgcttct ccgataaaat gtcagagaag
     8281 catgattacg tggttattgt ctgttgatga cttgttgacc gagccgtcat cattctcaat
     8341 gcattagtta aatcatttgt gatttttaag gattatcctg tctatgattt tctacttaga
     8401 aggatatggc actaactatg catcttatag tttactgctg gcatctattt aaaatagcaa
     8461 ttgagtcaaa tagcccatca agatagccat acctaatata accgtcagga atgcagctat
     8521 catcggcatt ctgaacattg atttaagtaa tatcacttcc gtcaggcttg cgccggcact
     8581 gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca gaacagatgc
     8641 cagagggata accgcctcag cccggatata cagcggaata ccgacaacgg cgcttaatgg
     8701 gatggcgaag ggattatctg ctcctgcatg agcggcaatc cacgctgagg gaataaagcc
     8761 ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat ctttaaactg
     8821 ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag ctttggtact
     8881 gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac aactcacttt
     8941 tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat ttgctgatga
     9001 gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa tactggcaag
     9061 aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac caaaggtcat
     9121 ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa aagtgagagt
     9181 cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg tcgagcaact
     9241 acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc cttttctggc
     9301 ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc tgacaccggc
     9361 actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca tctctgcg
//
LOCUS       DAJTRW010000131         9412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRW010000131 DAJTRW010000000
VERSION     DAJTRW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OO326_003556"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OO326_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5967809.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OO326_003557"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OO326_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5967810.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO326_003558"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO326_003558"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5967811.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO326_003559"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO326_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5967812.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OO326_003560"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OO326_003560"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5967813.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO326_003561"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO326_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5967814.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="OO326_003562"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="OO326_003562"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5967815.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJTRW010000132         9331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRW010000132 DAJTRW010000000
VERSION     DAJTRW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO326_003563"
     CDS             complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO326_003563"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5967816.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO326_003564"
     CDS             complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO326_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5967817.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO326_003565"
                     /pseudo
     CDS             complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO326_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4119..4874
                     /locus_tag="OO326_003566"
     CDS             4119..4874
                     /locus_tag="OO326_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5967818.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4953..5026
                     /locus_tag="OO326_003567"
     tRNA            4953..5026
                     /locus_tag="OO326_003567"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4985..4987,aa:Gly,seq:ccc)
     gene            5161..6771
                     /locus_tag="OO326_003568"
     CDS             5161..6771
                     /locus_tag="OO326_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5967819.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6803..7410
                     /locus_tag="OO326_003569"
                     /pseudo
     CDS             6803..7410
                     /locus_tag="OO326_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8341..9087
                     /locus_tag="OO326_003570"
     CDS             8341..9087
                     /locus_tag="OO326_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5967820.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2377 a         2304 c         2382 g         2268 t
ORIGIN      
        1 aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa
       61 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
      121 ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
      181 gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga tttcgcataa
      241 gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct
      301 tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac
      361 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt
      421 tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg
      481 ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg
      541 cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact
      601 ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag
      661 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
      721 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
      781 ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat
      841 atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga
      901 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
      961 cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg
     1021 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac
     1081 ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga tgacgttgag
     1141 ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat
     1201 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
     1261 aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg
     1321 cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
     1381 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat
     1441 aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
     1501 ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt atgtccgtat
     1561 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
     1621 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg
     1681 atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct
     1741 tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg
     1801 gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt
     1861 gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg
     1921 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
     1981 agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag catgactgcg
     2041 gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca
     2101 acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc ggtatcttgt
     2161 gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag
     2221 aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca
     2281 tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
     2341 gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat
     2401 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
     2461 cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg
     2521 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac
     2581 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
     2641 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
     2701 ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac
     2761 agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat ttgcccgtca
     2821 atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact
     2881 ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca
     2941 tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag
     3001 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
     3061 acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga
     3121 caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
     3181 gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc gccgttatgg
     3241 attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg taattcttgc
     3301 cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg
     3361 atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta
     3421 tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg aatatcaggc
     3481 acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg ttcatcatta
     3541 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc
     3601 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg
     3661 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt
     3721 tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac
     3781 gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac agatatgata
     3841 cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg ataaaatcaa
     3901 tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaactt
     3961 cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc
     4021 tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc
     4081 tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata
     4141 aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg ggctgttcgg
     4201 gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg
     4261 atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg gcgcgactga
     4321 acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga
     4381 aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc gcatcggtta
     4441 caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt tgttggttat
     4501 ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga
     4561 ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt
     4621 atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt
     4681 acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg
     4741 ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc
     4801 agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg cagggcagca
     4861 agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg ctaaggagag
     4921 cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacgaga
     4981 gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac acttcttaaa
     5041 gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata gttttactca
     5101 ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag gtctcagtac
     5161 atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat ccgtcttagg
     5221 atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta taaactagcg
     5281 tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg gcacatcaag
     5341 tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga gctacgggag
     5401 cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct gaacgacccg
     5461 gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct tcgggaagct
     5521 gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt tatcggtgtg
     5581 cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc ttaccttgag
     5641 cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga acctgaagtc
     5701 cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca ggagaacgcc
     5761 cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac gctaatcgag
     5821 atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga tatgctcaga
     5881 gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa gtctatgccg
     5941 ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt taccattaac
     6001 aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa cgacttgata
     6061 gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc ctccgacgct
     6121 cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa agcgtactcg
     6181 cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc gattacaggt
     6241 gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac tgaggctggg
     6301 accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag tatcaagaac
     6361 gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt attggctgac
     6421 tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat ggtgttcaat
     6481 ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt taaccgaacg
     6541 ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt tagacataca
     6601 gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc gattatgggt
     6661 catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt ggaaacgctg
     6721 aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta atcgcatagg
     6781 gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag aaaataagcc
     6841 ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa tggcttcgga
     6901 agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt ttgctattga
     6961 ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga cgcagaaaga
     7021 gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca attatatggg
     7081 gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta ttgctcacgg
     7141 cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc aagacgatat
     7201 tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt tgagcgcctt
     7261 gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa tggttacagg
     7321 ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc ttttggcgtc
     7381 agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat agaggaatgg
     7441 acccttaact atcactatag gggagagacc ctaagtcccc accttaatag cagtcacgac
     7501 ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga aaccatccgc
     7561 agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta agctatcttc
     7621 ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc tgtgataatc
     7681 gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc catttgtgga
     7741 caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt tgagtcttga
     7801 ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat cacaattcca
     7861 ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa ttatggtcgc
     7921 tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt cgcgtcaagt
     7981 tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat ctcgcctctg
     8041 acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta gccaccagcc
     8101 taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact taagtctatc
     8161 accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc agaccactaa
     8221 ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc cctttacgtg
     8281 ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga gtacacgtaa
     8341 atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat gacctcagtt
     8401 gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat tcgtaatatg
     8461 gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag cgagcttaac
     8521 gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg tgagagccga
     8581 acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta ttccgttcac
     8641 ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct tcaactggaa
     8701 gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac ttggggcgac
     8761 tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct ccagcatcgt
     8821 ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa gtattccgac
     8881 tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta taacttccgg
     8941 ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa gatgcgaggc
     9001 atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct gaagctggca
     9061 caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac ctgaggggcg
     9121 catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc ttggtctaga
     9181 cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc aactggtcgc
     9241 tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt taaacacctt
     9301 ttgagatgac cgatgcgggg agacccgtag g
//
LOCUS       DAJTRW010000133         9292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRW010000133 DAJTRW010000000
VERSION     DAJTRW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..257
                     /gene="mliC"
                     /locus_tag="OO326_003571"
     CDS             <1..257
                     /gene="mliC"
                     /locus_tag="OO326_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5967821.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            316..972
                     /gene="pdxH"
                     /locus_tag="OO326_003572"
     CDS             316..972
                     /gene="pdxH"
                     /locus_tag="OO326_003572"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5967822.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1101..2375
                     /gene="tyrS"
                     /locus_tag="OO326_003573"
     CDS             1101..2375
                     /gene="tyrS"
                     /locus_tag="OO326_003573"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5967823.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2434..3297
                     /gene="pdxY"
                     /locus_tag="OO326_003574"
     CDS             2434..3297
                     /gene="pdxY"
                     /locus_tag="OO326_003574"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5967824.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3341..3946)
                     /gene="gstA"
                     /locus_tag="OO326_003575"
     CDS             complement(3341..3946)
                     /gene="gstA"
                     /locus_tag="OO326_003575"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5967825.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4052..5554)
                     /gene="dtpA"
                     /locus_tag="OO326_003576"
     CDS             complement(4052..5554)
                     /gene="dtpA"
                     /locus_tag="OO326_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5967826.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="OO326_003577"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="OO326_003577"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5967827.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="OO326_003578"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="OO326_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5967828.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="OO326_003579"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="OO326_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5967829.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="OO326_003580"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="OO326_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5967830.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2225 t
ORIGIN      
        1 ttgagcgaat gcaaaccgat acgctggaat accagtgtga tgaaaaaccg ttgacggtca
       61 aactgaataa tccgcgccag gaagtcagtt ttgtttacga taatcaacta ctgcatctca
      121 aacagggcct ttcagcctct ggcgcgcgtt actccgacgg gatctatgtt ttctggtcga
      181 aaggtgagga agcgactgtc tataaacgcg accgcatcgt cttgaataac tgtcagttac
      241 aaaatccaca gcgttgagat ttttccaggg gcggcgcaca atagcgtcac ccactgacaa
      301 tccgtaaaga aaaccatgtc tgataacgac gaattgcagc aaatcgcgca tctgcgccgt
      361 gaatacacca aaggcgggtt acgccgccgc gatcttcccg ccgatccatt aacccttttt
      421 gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg accctaccgc gatggtggtc
      481 gctaccgtgg atgaacatgg tcagccttat cagcgcatcg ttttactcaa acattacgac
      541 gaaaaaggca tggtgtttta caccaacctc ggtagccgta aagcacatca aattgaaaat
      601 aatccgcgcg ttagcctgct gttcccgtgg catacccttg aacgccaggt gatggtgatc
      661 ggtaaagcag aacgactttc gactctcgaa gtgatgaaat attttcatag ccgcccgcgt
      721 gatagccaga ttggtgcatg ggtttcgaag cagtccagtc gcatttctgc ccgcggtatc
      781 cttgaaagta aattcctgga gctgaagcag aagtttcaac agggcgaagt gccattgccg
      841 agcttttggg gcggttttcg cgtcagcctt gaacagattg agttctggca gggcggtgag
      901 catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg atgcgtggaa gattgatcgt
      961 cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc tggtactcct gattctggca
     1021 ctttattcta tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa ggtgcagtcg
     1081 ttatatacat ggagattttg atggcaagca gtaacttgat taaacaattg caagagcggg
     1141 ggctggtagc ccaggtgacg gacgaggaag cgttagcaga gcgactggcg caaggcccga
     1201 tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt gcatttgggg catcttgttc
     1261 cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg ctggtaggcg
     1321 gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg
     1381 aagaaactgt tcaggagtgg gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt
     1441 tcgactgtgg agaaaactct gctatcgcgg cgaacaacta tgactggttc ggcaatatga
     1501 atgtgctgac cttcctgcgc gatattggca aacacttctc cgttaaccag atgatcaaca
     1561 aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg gatttcgttc actgagtttt
     1621 cctacaacct gttgcagggt tatgacttcg cctgtctgaa caaacagtac gatgtggtgc
     1681 tgcaaattgg tggttctgac cagtggggta acatcacttc tggtatcgac ctgacccgtc
     1741 gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcaccaaa gcagacggca
     1801 ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa accagcccgt
     1861 acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc ttcctgaagt
     1921 tcttcacctt tatgagcatt gaagagatca acgccctaga agaagaagat aaaaacagcg
     1981 gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gactcgtctg gttcacggtg
     2041 aagaaggttt acaggcggca aaacgtatta ccgaatgcct gttcagcggt tctttgagtg
     2101 cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg
     2161 aaaagggcgc agacctgatg caggcgctgg tcgattctga actgcaacct tcccgtggtc
     2221 aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc
     2281 ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg
     2341 gcaaaaagaa ttactgtctg atttgctgga aataatgcat taagtggaaa gggggagtga
     2401 gaaatcactc cccctggttt ttatacaggg aacatgatga aaaatattct cgctatccag
     2461 tctcacgttg tttatggtca tgcgggtaac agtgcggcag agtttccgat gcgccgcctg
     2521 ggcgcgaacg tctggccgct gaacaccgtt caattttcta atcacaccca atacggcaaa
     2581 tggactggct gcgtgatgcc gcccagccat ttaaccgaaa ttgtgcaagg cattgccgcc
     2641 attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc ggcggagcag
     2701 ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag cgaatccgca ggcgaaatat
     2761 ttttgcgatc cggtaatggg tcatccggaa aaaggctgta tcgttgcacc gggtgtcgca
     2821 gagtttcatg tgcggcacgg tttgcctgcc agcgatatca ttgcgccgaa tctggttgag
     2881 ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag aagcggttct ggcagcgcgc
     2941 gaactcattg agcaagggcc acaaattgtg ttggttaaac acctggcgcg agctggctac
     3001 agccgtgacc gttttgaaat gctgctggtc accgccgatg aagtctggca tatcagccgt
     3061 ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac gagcggttta
     3121 ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca tgtgaccgct
     3181 gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagct gcaagtggtg
     3241 gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa gctctgaaat
     3301 atctcatttc ggcccacaac gacctgtggg ccgatggcta ttactttaag ccttccgctg
     3361 acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc gcaatgtgct
     3421 ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagaaccgta aacagatagg
     3481 catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt gcctcgttca
     3541 catattgcag cttcttatcc agctgcgcgc gaactgtcgg tttgtactct tccggtgtat
     3601 ctgggcgaaa cagaggtgtg aaacctttat gcagctcggt ggcgatgtaa ttcagccatt
     3661 cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg tcggggacgc
     3721 tgtcggcaag atactgcata atcgcaacgc cttccgtcag caaagtacca tcgtccagca
     3781 gcaatgcagg cacctgcccc ttagggttaa cggaaaagta atcgtcaccg ttttcgagac
     3841 gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca cgcagggtga
     3901 tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt aactcctttt
     3961 gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaagccgc taacgaaaag
     4021 ttagcggctt gtaaaagagt ttccctgaat tttacgctac agtagctttc gccgctttgt
     4081 ctgcagcgtc atcctgcgtc atgcggtgca gtttaggcgc ggtcagcagc atcagtactg
     4141 caataacggc agtagcgaca ccaatctgca agaatacgcg accatagact tccagtgaca
     4201 tcagcgggtc ggtaacgtta tccggcacag ccatcatacc cgcaacataa ccaccaatca
     4261 ggtttgcacc ggcagtggtc aggaaccagc tacccataat gaagcccatc agacgctgcg
     4321 gaacgagttg tgcaaccatt gccagaccca gaccagagat catcagttcc ccgatgctct
     4381 gcaggccata gcttgcgacc agccagctga cagacacgat acctgcgtca gacgcgaatt
     4441 tcgcacccag cggcagaatc aggaacgcac cagaacacat caccatgccg attgcaaact
     4501 tggttggcat cggcagggta tcacccatct tgttatagat agcggccaga atcggactac
     4561 cgatgatgat ccagaacggg ttcagtgcct gatactgctc aggttctacg gccagaccca
     4621 gaatggagtg ctcaacgtta cgaatcgcaa agaagttcag tgacgttggc atctggctgt
     4681 acagcacgaa gaagatgatt gcttcgagca tcaggatgaa ggcaacgatc attttacgac
     4741 gcgcagcacc tttcatcgcg aaagcttctt taccgaagat aaccacaata ccgaaggcaa
     4801 caacgcccag cgccatacgc gcaacttcct gattgtgcag tagccaggtg gcaacagcga
     4861 tcagcgccac aacaccaata atggtcagca acaggttacg gtagttgact ggttcaaagt
     4921 ctggttttga accgtactgt ttaacccagc gttggcagaa ggcgaagtta acgatagtga
     4981 tcagcaggcc tacaacgctc aacgcaaacg caacactcca gccgtatttc gcggccagcc
     5041 acggggtggc tatcatagag aagaaagagc cgatgttgac ggacatgtag tacatggtga
     5101 atgcaccgtc cagacgtggg tcgtttttct catagcatgt agaaagcaga gaagacgggt
     5161 tagctttaaa caggccgtta ccgaccgcaa tagccgccat acccatataa acaataccgg
     5221 cgtcgtgacc agaccaggca accagagcat aaccaatcgc cagcacaata gcgccgagca
     5281 taattacgcg tttagtaccc agtaccttgt cacctaacca gccgccgata gcgaccagac
     5341 cataaaccag ggcactaaag gaagagaaaa gggtgattga atccgcttca gacataccca
     5401 gttgtttaac caggtaaaca gccataattc cttgtaggcc gtaataacca aaacgttccc
     5461 ataactcaat cgagaagatg agatagaacg ccttcggttg tttgaaagcg ttcaaactga
     5521 cgctttcagt tggtttttgg tttgcagtgg acacttttac ctcttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca tt
//
LOCUS       DAJTRW010000134         9135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRW010000134 DAJTRW010000000
VERSION     DAJTRW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..177)
                     /locus_tag="OO326_003581"
     CDS             complement(<1..177)
                     /locus_tag="OO326_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967831.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYL"
     gene            697..1392
                     /gene="minC"
                     /locus_tag="OO326_003582"
     CDS             697..1392
                     /gene="minC"
                     /locus_tag="OO326_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5967832.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1416..2228
                     /gene="minD"
                     /locus_tag="OO326_003583"
     CDS             1416..2228
                     /gene="minD"
                     /locus_tag="OO326_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5967833.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2232..2498
                     /gene="minE"
                     /locus_tag="OO326_003584"
     CDS             2232..2498
                     /gene="minE"
                     /locus_tag="OO326_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5967834.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2870..3214)
                     /locus_tag="OO326_003585"
                     /pseudo
     CDS             complement(2870..3214)
                     /locus_tag="OO326_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3249..3368)
                     /locus_tag="OO326_003586"
     CDS             complement(3249..3368)
                     /locus_tag="OO326_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5967835.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3329..3514)
                     /locus_tag="OO326_003587"
     CDS             complement(3329..3514)
                     /locus_tag="OO326_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967836.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3615..3788
                     /locus_tag="OO326_003588"
     CDS             3615..3788
                     /locus_tag="OO326_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967837.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3790..4134
                     /locus_tag="OO326_003589"
     CDS             3790..4134
                     /locus_tag="OO326_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5967838.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4138..4473
                     /locus_tag="OO326_003590"
     CDS             4138..4473
                     /locus_tag="OO326_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5967839.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4530..6851)
                     /locus_tag="OO326_003591"
     CDS             complement(4530..6851)
                     /locus_tag="OO326_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5967840.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6968..7177)
                     /locus_tag="OO326_003592"
     CDS             complement(6968..7177)
                     /locus_tag="OO326_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967841.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7928..>9135)
                     /locus_tag="OO326_003593"
     CDS             complement(7928..>9135)
                     /locus_tag="OO326_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5967842.1"
                     /translation="LLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTIS
                     DQHIRGALNVPLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNP
                     PPLFSFYRLIDKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFY
                     QPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQM
                     NAIASKLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVD
                     ESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTR
                     ILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILS
                     KPKVKVVVE"
BASE COUNT         2581 a         2121 c         1987 g         2446 t
ORIGIN      
        1 gagatatttc cttgttaact ctggggaaat gccatctgag ttcacacaaa tatacgggtc
       61 gcaaacaacg ccgcacgtcg gagattgaag tccgccagcg tcatgtccag acgccattga
      121 tgagaacatc atccccacac ctgacaatcc gatataaata atacgcatca tattcatagc
      181 acatcctgat aatccaaccg tacaacactt atgctcattt cgtcatcaat ccgtaatcaa
      241 aaccgctgac ctggacgagt tacgatgacc tgacagaagt atcagacgtc ttctgcggtt
      301 tctatgatag agcaattctg tggcggatat taagataacg ggtatctgtt ctttaatgat
      361 gctgcaaaaa atggaaagtt aagaaagcct ggcgtggtga aaaaatacac ttgcctcaat
      421 actggatatt taaacaggtc tggagatgct gtcacgatta gggtgcggaa aaaggaagcg
      481 taaataagaa gaatcaaaag tacgacggca atgggttgat tgacaagggt attttttaag
      541 ctatgaatca gcgccattta tcacagaata gacttttact ctgaataaat gggagggtga
      601 cttgcctcaa tataatccag actataacat gccttatagt cttcggaaca tcatcgcgcg
      661 ctggcgatga ttaatagcta attgagtaag gccaggatgt caaacactcc aatcgagctt
      721 aaaggcagta gcttcacttt atctgtggtt catctgcatg aggcagaacc taaggttatc
      781 catcaggcgc tgaaagacaa aatcgctcag gcccccacat ttttaaaaca tgcccccgtt
      841 gtactcaacg tcagtgcact ggaagacccg gtaaactggt cagcgatgca taaggcggtt
      901 tcggcaaccg gtttgcgggt tattggcgta agtggctgca aagatgcgca acttaaagcc
      961 gaaattgaaa aaatggggct gcctatcctg acggaaggaa aagaaaaagc gccacgtcca
     1021 gctcccgcac cgcaggctcc agcgcaaaat acaatgccgg tcacaaaaac gcgtttaata
     1081 gataccccgg tgcgttccgg tcagcgtatt tatgctccac aatgtgatct gattgttaca
     1141 agccacgtta gcgctggggc cgaattgatt gccgatggga acattcatgt ctatggcatg
     1201 atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg aaacgcaaat attttgtacg
     1261 aacctgatgg cggaattggt gtccatcgca ggtgaatact ggctgagtga tcaaatccca
     1321 gcagaatttt atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa
     1381 ccgttaaatt gatccctttt taacaaggaa tttctatggc acgcattatt gttgttactt
     1441 cgggtaaagg gggtgttggt aagacaacct ccagcgcggc catcgccact ggtttggccc
     1501 agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga
     1561 ttatgggttg cgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa
     1621 cgctaaatca ggcgttaatt aaagataagc gtactgaaaa tctctatatt cttccggcat
     1681 cgcaaacacg cgataaagat gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc
     1741 tgaaagcgat ggattttgaa tttatcgttt gtgactcccc ggcagggatt gaaaccggtg
     1801 cgttaatggc actctatttt gcagacgaag ccattattac caccaacccg gaagtctcct
     1861 cagtacgcga ctctgaccgt attttaggca ttctggcgtc aaaatcacgc cgcgcagaaa
     1921 atggcgaaga gcctattaaa gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa
     1981 gcagaggtga catgctgagc atggaagatg tgctggagat cctgcgcatc aaactcgtcg
     2041 gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc
     2101 tcgacattaa cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt ctgttgggag
     2161 aagaacgtcc tttccgcttc attgaagaag agaagaaagg cttcctcaaa cgcttgttcg
     2221 gaggataagt tatggcatta ctcgatttct ttctctcgcg gaagaagaac acagccaaca
     2281 ttgcaaaaga acggctgcag attattgttg ctgaacgccg tcgcagcgat gcagaaccgc
     2341 attatctgcc gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac gtacaaattg
     2401 atcctgagat ggtaaccgta cagcttgagc aaaaagatgg cgatatttct attcttgagc
     2461 tgaacgtgac cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa aacgcattta
     2521 tcttcaaggc agagtcattt ctgccttgag tttttcatcc ctctcatcca cgttgtggta
     2581 aagcggcgag tattcttgct gatactcctc attgctattt ccacctcccc ctttacacct
     2641 taaggctgtt cccacgcaca gaacaaaatg tttatgcctg gttgagtaaa taaccatgtt
     2701 attagtgtgt atatactggg agatgataac cccgcaattt cattattcat tgttctccca
     2761 taggatgagg tttcccgccg taactggcgg gctttttttg ccccagaatt ttctctttca
     2821 acatcctgta aacgaaaact gcgccgaagc gcagtttaat cgttgatggt cagaacgacc
     2881 agttcacacc agccaccgcg ttccacgggg actccacacc ggcaccatgg ctatacccca
     2941 ccccgaaatg cccgcttaac gtactgctga atgaggcttt aatacctgcc tggtatattc
     3001 cacgtctgcc cgacaaatca ttgacaaaat taccgtcact attcactttc acccgattat
     3061 catcgacaaa ttctttgcgc acagccgcct tcaggcaggg ttcaatttcc ataccgttcc
     3121 ccagacgcat gttgtaactc agcgttgcac ccagttcacg atatatactg cgggtatcga
     3181 ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac ctaagctctg
     3241 ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt
     3301 gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa
     3361 agaggcattt tttacacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca
     3421 gataatactg tcgtacgctt atctttagac aatgaggccg tcggaaaaat aaaatgacta
     3481 atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat aatattgata
     3541 aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt cttgaataaa
     3601 aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac gtaaaaaaca
     3661 taattcctat catcacagcc acgctcattt tgaattatct gaataatagc gagcgttctt
     3721 tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg
     3781 acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc
     3841 acgccagcga tagcggatat gaaccgcacc acgaaaggtg cattgctagg cgcaggcgtt
     3901 ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt
     3961 gttagtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagtg
     4021 ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt
     4081 aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg
     4141 atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat gacctgggcg
     4201 caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc
     4261 aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat
     4321 gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag cgccaaagtg
     4381 gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt
     4441 gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat
     4501 acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc caccatcact
     4561 gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca gacgctggcc
     4621 gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact caccccatct
     4681 tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg gatccagttc
     4741 atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga
     4801 ataaaggtgc ggaagccgag tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt
     4861 gagccattgg cttccgtgtg cgcattatga tcgacgccca tccagatagc ctgtgcctgt
     4921 ggctggaaga tccacgtata tttcgccgct tccagaccaa aggatttatt cagggcaaaa
     4981 ccgtaacccg cttccagcga gacggttgca cctttcgcgc tataggattc ataggctaat
     5041 tcatcccctt tcacggagtt tttaaaccag ctatattgcg cccaggagtc aatgtatgcg
     5101 ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgct atagcctctg
     5161 acactccctt tcgaacgata atccgacacg ctggaatgag ttaagttgta gtcgcgggca
     5221 taaccagcca tcactcccaa acgccaactg tcactatcgg taaaaccacc ggttaacaga
     5281 tcgcccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc gttgctgtca
     5341 cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg ctcctgttca
     5401 gttacaggat cgatgtagcg cgtttcaccc gcacggtcgt tcagacgcat aacaaaaaga
     5461 gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cggggcgcag gacgggggta
     5521 ggcgcaacag taggcgctga acttcctccg gagtttgttt caccgccgct cgttgcctgg
     5581 aagttagtca gataccagtt tttattggtc gcttcggtat tacctcgggt taatgaatat
     5641 tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt ccccatttga ttccccctca
     5701 acgctgataa tctctatccc atttaacgtg taagctccgc tgcctcctgc attggtaact
     5761 ttaactcggg ttgttccaga ggtatttcca ttaataacga ctttatccgt agctgaatta
     5821 tcatcatcca attcagttct gagatgcaat acgccattat taccaacgta attttcagtt
     5881 attgttaatc ttgttggttc gacatcccgt ccatctgccc gggaaatata gaccgtacta
     5941 ttatcaaccg tgaggttgct caccgttgag tcagctttaa tatcccaggt actattatct
     6001 gaaagcgaca gataggtatg agtgttatca tctgttgaaa tattagctga acccgttatt
     6061 tttgaattag ttgcatcgac ggtcaaatga cctttaatat tattgataaa aacatctgca
     6121 gccgatgata cctcactatc cgtaatggtc acgttactcg tctcatcttt atggtagagg
     6181 atttcatttg ctgaccgtag tgagctacca gataaaatat ctaccgttgc ttcctgacca
     6241 tttatggaag actccagcac aattccagac ccctgatgcg cgactgcaga tgtatttttc
     6301 agcgtcaccg tgccgccatt ataaatttta gcaatcgcgg cgtcaggacc cgttgtataa
     6361 atttctccct ggttaaatga aactgtcccc tggcgggcag cgatggcatg agcattgtcc
     6421 ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg cattggtata atcaagcgtg
     6481 tcaccagcct tgacgacagt gccattagcg tacgcggcat aactttctgt tccgtgggtt
     6541 gtgatctccc cttttgccat atccagtttt gctatggcat tattttcatt acttcctgta
     6601 tcggcagtac tttcttcatc aagagaagat acacacgcac acgcgcttcc gggagatgca
     6661 cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa taaatgtatc atctccgtca
     6721 gaaaaagagg gggtaaccga accgtcagag ttgactctta ttatggtgtc agtattcagg
     6781 tttccactcg tttgacttag caaaccaatt gagtaatccc ccgttgtgac aatcgtgttt
     6841 ttaccctgca aaataacagc accgccaaat tcacttacga ttgcgctagc tgttaatgct
     6901 gggcttagag gactattgtc agaaggatat aaaccgcccg ttgttacaat gacgccattg
     6961 ttaatggtta acttactacc ctctcctgca acggataccc gtaacttcca ttacccgttg
     7021 ttataactag cgcattatca atatctttta cttttccatc ttttattgcc acagcatgtt
     7081 tatattcatc gtgctgaata aactgttcag catatgcatt acccaccgcc agagcaataa
     7141 gtcccaaact aaacccgggg agttttttca gtttcataga ttaactatcc attcattgga
     7201 ttaacaaaca ttcctggtca aataacctat gagctcagat taccaaaacg gagggtaaag
     7261 acaaaaacaa aagcacaaaa aatttaaata gataaacagc gacaatatat aaatgataaa
     7321 taagtcggca ttatcatttt tcaccttatt ttcatgacat tgatcacttt ggggtgacta
     7381 ttttgtttaa caaataactc ttcgacatca aaattattaa tagttgccat ggtaattatt
     7441 tcggtgatat ataaaaacaa ctgtgataac accaaggttg acaactattt gcgcaataat
     7501 tcctttcctt caatgatcca ggcgttcact gccagcaacc cattcagccg catactgaca
     7561 gtgagaaact gaaagattat tgctgatccc tgtggctaac gagtctttct cttaaatcga
     7621 ttaaccagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt cacaagcact
     7681 tctcccagcc cggtactggg ttttgcaata aggatgacca ggagggtgat cagcataaat
     7741 gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt attcatgcta
     7801 ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa tgaacaatag
     7861 taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg gccttactga
     7921 tcaattttca ctcaaccaca accttcacct tcggtttaga aaggataatt ttgaccaggt
     7981 cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata ttattttggt
     8041 tcagatagcc aagttgttct ttcgtttcga caccttcagc gacgatactg atcgaaagtt
     8101 tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct gcgtttacgc
     8161 ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatga agataagaaa
     8221 gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt tcatgcagaa
     8281 tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg ctcacgctcg gtgacttcca
     8341 gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa atttaaacac tcgtcgacaa
     8401 acgtcggcga aataatatgc gaagcgctaa aattaattcc aatatgaaaa ccttccggca
     8461 gtttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt tgtgtaagcg
     8521 gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgaa atatatccac
     8581 cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct tcccgaccat
     8641 ttaccacagg ttggtaaaaa gggacgatct ccccattatt tattgcccgt cgcaggatct
     8701 cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag gaaggcagcg gcgctggcga
     8761 ttaacagaat aaatattaaa atcccaaagc ctttatcgat aagtctgtag aagctaaaga
     8821 gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc ttctcggtat
     8881 agggataatg tccattaagc gtcatcacat cagccgttgg tccaataatg tcatccgcca
     8941 cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga atatgttgat
     9001 cacttatcgt aaccaaaatg cgcgtatctg caaattggct ctgatagagc aggataggta
     9061 atctattaac ggtgtcgatt gccggagcca acagtaaatt actgtccggg acaacaggaa
     9121 tacgagacag cagga
//
LOCUS       DAJTRW010000135         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRW010000135 DAJTRW010000000
VERSION     DAJTRW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO326_003594"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO326_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5967843.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO326_003595"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO326_003595"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5967844.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO326_003596"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO326_003596"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5967845.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO326_003597"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO326_003597"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5967846.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO326_003598"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO326_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5967847.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO326_003599"
     CDS             5562..6035
                     /locus_tag="OO326_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5967848.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO326_003600"
     CDS             6064..6606
                     /locus_tag="OO326_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5967849.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO326_003601"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO326_003601"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5967850.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO326_003602"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO326_003602"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5967851.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO326_003603"
     CDS             8003..8260
                     /locus_tag="OO326_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5967852.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO326_003604"
     CDS             complement(8236..8790)
                     /locus_tag="OO326_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5967853.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2192 c         2252 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTRW010000136         8960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRW010000136 DAJTRW010000000
VERSION     DAJTRW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..699
                     /locus_tag="OO326_003605"
     CDS             <1..699
                     /locus_tag="OO326_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5967854.1"
                     /translation="AWYRNRAQFVLTDEERTYLSASVKPRPQLQFSEWLALFKHRTTW
                     GMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVGMWVNGIVVD
                     RLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALFCVHFAGTSA
                     WGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLALVIAACVTFT
                     GALCYFFIVKDRIE"
     gene            951..1868
                     /gene="oxyR"
                     /locus_tag="OO326_003606"
     CDS             951..1868
                     /gene="oxyR"
                     /locus_tag="OO326_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5967855.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1851..3251)
                     /gene="sthA"
                     /locus_tag="OO326_003607"
     CDS             complement(1851..3251)
                     /gene="sthA"
                     /locus_tag="OO326_003607"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5967856.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3572..4222
                     /gene="fabR"
                     /locus_tag="OO326_003608"
     CDS             3572..4222
                     /gene="fabR"
                     /locus_tag="OO326_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5967857.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4222..4581
                     /locus_tag="OO326_003609"
     CDS             4222..4581
                     /locus_tag="OO326_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5967858.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4621..5721)
                     /gene="trmA"
                     /locus_tag="OO326_003610"
     CDS             complement(4621..5721)
                     /gene="trmA"
                     /locus_tag="OO326_003610"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5967859.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5835..6025
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6090..7934
                     /gene="btuB"
                     /locus_tag="OO326_003611"
     CDS             6090..7934
                     /gene="btuB"
                     /locus_tag="OO326_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5967860.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7879..8736
                     /gene="murI"
                     /locus_tag="OO326_003612"
     CDS             7879..8736
                     /gene="murI"
                     /locus_tag="OO326_003612"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5967861.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2060 a         2171 c         2409 g         2320 t
ORIGIN      
        1 gcgtggtatc gcaaccgggc gcagtttgtc ctgactgacg aagaacggac gtatctctcc
       61 gcgtcggtta aaccgcgtcc acagctgcaa tttagcgagt ggctggcgct gtttaagcat
      121 cggacaacct gggggatgat tttaggtttc tctggcgtca attataccgg ttggctttac
      181 attgcgtggt tacccggtta tttgcaagcc gaacaaggtt tcagtctggc gaaaaccggt
      241 tgggtagcgg cgattccttt cctcgcggcg gcggtcggga tgtgggttaa cggtattgtg
      301 gtcgatcggc tggcgaaaaa aggctacgac ctggcgaaga cgcgtaaaac ggctattgtc
      361 tgcggtttga tgatgtcggc attaggcacg ttgctggtcg tgcaatcttc ctcgccagct
      421 caggcggtag cgtttatctc aatggcgctg ttctgcgtgc atttcgcggg aacgtctgca
      481 tggggactgg tgcaggtgat ggtgtcagaa acaaaagtgg cttccatcgc cgggattcaa
      541 aactttggca gttttgtttt tgcttccttt gctccgatcg taaccggttg ggtagtggat
      601 accacacact cgtttaatct ggcgctggtt atcgcggcct gtgtgacgtt caccggggcg
      661 ctgtgttact tctttattgt caaagatcgc attgagtaat cgttactatc aggctctctt
      721 gctgtgggcc tgtagaataa aaaaaagcgg atcctggaga tccgcaaaag ttcacgttgg
      781 ctttagttat tcgagttgag aaactctcga aacgggcagt gacttcaagg gttaaaagag
      841 gtgccgctcc gtttctgtga gcaattatca gtcagaatgc ttgataggga taatcgttca
      901 ttgctattct acctatcgcc atgaactatc gtggcgatgg aggatggata atgaatattc
      961 gtgatcttga gtacctggtg gcattggctg aacaccgcca ttttcggcgt gcggcagatt
     1021 cctgccacgt tagccagccg acgcttagcg gccaaattcg taagctggaa gatgagctgg
     1081 gcgtgatgtt gctggagcgg accagccgta aagtgttgtt cacccaggcg ggaatgctgc
     1141 tggtggatca ggcgcgtacc gtgctgcgtg aggtgaaagt ccttaaagag atggcaagcc
     1201 agcagggtga gacgatgtcc ggaccgctgc atattggttt gattcctacg gttggaccat
     1261 acctgctacc gcatattatc ccgatgctgc accagacatt tccaaagctg gaaatgtatc
     1321 tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaaa ctcgattgcg
     1381 tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccgttg tttgatgagc
     1441 caatgttgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc gtactgatgg
     1501 ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg tcactgtttg cgcgatcagg
     1561 caatgggttt ctgctttgaa gccggggcgg atgaagatac acacttccgc gcgaccagcc
     1621 tggaaactct gcgcaacatg gtggcggcag gtagcgggat cactttattg ccagcgctgg
     1681 ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct gccatgcatt aagccggaac
     1741 catgccgcac tattggcctg gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc
     1801 agctggcaga ggccatccgc gcaagaatgg atggccattt cgataaagtg ttaaaacagg
     1861 cggtttaaac cgtttaacgc agctacccga taggcttccg ccatcgtcgg gtagttaaag
     1921 gtggtgttga cgaagtactc aatagtgttg ccgccacctt tctgttccat aatcgcctga
     1981 ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt gaatacccag aatctctttt
     2041 gtttcccgat ggaacaaaat tttcagcgtg cccacgttca tgccgacgat ttgtgcacgt
     2101 gccagatgtt tgaactgggc gcggcccact tcatacggca ctttcatcgc ggtcagctgc
     2161 tgttcggttt tgcccacaga gctgatttcc gggatggtgt aaatgccggt agggatatct
     2221 tcaatcagat gtgcggtggc ttcgcctttt accagcgcct gcgcggcaat gcgcccctgg
     2281 tcataggccg ctgatgccag gctcggataa ccaatcacgt cgcccaccgc gtaaacgtgc
     2341 ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc cgcgactgtc agtttccagc
     2401 ccaatgttct gtaacgccag cgaatcggta ttaccggtac gaccgttggc atagagcagg
     2461 cagtcagctt tcagtttttt acccgacttc agatgcatga tcacaccgtc gtcacagctt
     2521 tcgatcttct cgtactcttc gttgtgacga atcactacgc cactgttcca gaagtggtag
     2581 gagagagaat ctgacatctc ttgatcgaga aatgccagca ggcgatcgcg ggtgttgatc
     2641 agatccactt ttacatccat accgcggaag atcgacgcat attcacagcc gatcactcca
     2701 gcaccataga taagtacatg gcgcggttcg tggtgcatgc taagaattga gtcgctgtcg
     2761 taaatgcgtg gatgggtgaa atcaacatct gttggatgat atggacgaga gccgcaggca
     2821 ataacaaatt tttcagcggt tagtgtttca acgctgccgt ccgggcaatc cagcgccaac
     2881 gtatgctcgt caacaaagcg agcgttcccc tgcaatattt cacagtgatt acgttcgtaa
     2941 aatccctgac gcatgcgcgt ttgttgatta atcacgttat cggcatggtt aaggatatcg
     3001 gcaaaagaag agcggagcag tcgggaatgg tcgctgtata gtgggttttg attgaattct
     3061 ataatgcggc tgacggcgtg acggagagct ttcgacggga tggtgcccca gtgggtgcaa
     3121 ccgccgccaa cattttgata acgctcgata actgcgacgc gcgcaccttg cttaaccagg
     3181 cccattgcag cgccttcgcc gccggggccg gaacctatta ctatggcatc gtaatcgtag
     3241 gaatgtggca tggtagggct tacctgttct tatacataaa agcaacagaa tggtaacatt
     3301 ctatcgcggg taagccaatt gatccccgtc atttatctgg ctatatcctg agcggccttt
     3361 gctttgtctg tttcttactt ttgccctgac gttttattgg atttttatcg acgatactct
     3421 ccgtttaagc ggcaggtttc cgctgtacgt aaaagaaccg gccagtaaat atgttttatt
     3481 gcgttaccgt tcattcacaa tactggagca atccagtatg ttcattctct ggtatagtgc
     3541 cagcagtact tttggcaagg attcagacat cgtgatgggc gtaagagcgc aacaaaaaga
     3601 aaaaacccgc cgttcgctgg tggaagccgc atttagccaa ttaagtgctg aacgcagctt
     3661 cgccagcctg agtttgcgtg aagtggcgcg tgaagcgggc attgctccca cctcttttta
     3721 tcggcatttc cgcgacgtag acgaactggg tctgaccatg gttgatgaga gcggtttaat
     3781 gctacgccaa ctcatgcgcc aggcgcgtca gcgtatcgcc aaaggcggga gtgtgatccg
     3841 tacctcggtc tccacattta tggagttcat cggtaataat cctaacgcct tccggttatt
     3901 attgcgggaa cgctccggca cctccgctgc gtttcgtgcc gccgttgcgc gtgaaattca
     3961 gcacttcatt gcggaacttg cggactatct ggaactcgaa aaccatatgc cgcgtgcgtt
     4021 tactgaagcg caagccgaag caatggtgac aattgtcttc agtgcgggtg ccgaggcgtt
     4081 ggacgtcggc gtcgaacaac gtcggcaatt agaagagcga ctggtactgc aactgcgaat
     4141 gatttcgaaa ggggcttatt actggtatcg ccgtgaacaa gagaaaaccg caattattcc
     4201 gggaaatgtg aaggacgagt aatgaaacaa gcaaatcaag atagaggtac gctgctgctg
     4261 gcgttggttg ctggtttatc gattaatggt actttcgcag cgctgtttag ctccattgtg
     4321 ccattttctg tattcccgat tatttccctg gtgctgacgg tttactgcct gcatcaacgt
     4381 tatcttaatc gcaccatgtc ggtaggcttg ccgggtctgg cagctgcctg ttttattctc
     4441 ggcgtactgc tgtacagcac ggtagttcgt gcggaatatc cggatatcgg ctctaacttc
     4501 ttcccggcag tactctccgt cattatggtg ttctggattg gcgcgaagat gcgtaaccgt
     4561 aagcaggaag ttgctgagta atcggtatca tgccggatgc tggcgcatcc ggcatgggtt
     4621 ttacttcgcg gtcagtaata cgccgcactc catatggtgc gtgtagggga actgatcaaa
     4681 cagagccaga cgttcgacct tgtgcgtctg gcttaatgtt tccagatttt tgcataacgt
     4741 ttccggattg caggagatat acagaatacg tggatacgcc tgcaccattt tctcggtttc
     4801 actgtccaga ccgctgcgcg gtgggtcgac aaaaatggtt tcgaactgat agctctttaa
     4861 gtcgatccct tgcaggcggt taaactcgcg cacaccattc atcgcctgag taaattcttc
     4921 tgccgccata cgaataattt gtacgttatc aatatggtta gcggcaatgt tgtattgcgc
     4981 ggcagcgact gacggtttgg cgatttcggt ggctaatacc cgatcaaaat tgcgcgccag
     5041 cgctaatgaa aagttaccgt tgccgcagta cagctccagt aaatcgcctt ttgagccttt
     5101 ggttacgtcc agcgcccatt ccagcatctg aatattcatc gccgcgttcg gctgggtaaa
     5161 gctgttttct acctgacgat agatcatctc tttccctgcg accggcagac gctcgtcgat
     5221 gtaatcttga tccagcgcga ttttggtttt cgttgcccga ccaatcagat gcacattcag
     5281 attctgcgcg cgcagtgcat cgcgcagtgc ttccgcttcc tgacgccact catcatccag
     5341 ctttttatgg tacagcaggg aaaccaccgc ctgattactc agcgtggtga ggtaatcaat
     5401 ctggaacaac ttgtggcgca gaacgggatt attacgcaca cccgcaatca tcgccgtcat
     5461 caactggttg atgagttcac tggcggcggg gaagctatcc acgcggatgc ggcttttggt
     5521 ttgttgatcg aaaatgatgt gatacaggtc atcgccatcg tgccagatgc ggaactccgc
     5581 gcgcatccgg taatgactga ccggcgagcg aaacacttcc ggaaccaggt cagaaaacgg
     5641 tgccatcata ctttgcaaac gtaccacttt ttcggctaac tgcgcttcat actgttctgt
     5701 tggaaggtgt tcgggggtca tgatgtatcc tgaaaaatta aagtacgcgg cgattgtagg
     5761 gattgctcat cagatgtcca gatcttgatg aattcctatt tgtgagctac gtctggacag
     5821 taacttgtta caacctgtag catccacttg ccggtcctgt gagttaatag ggaatccagt
     5881 gcgaatctag agctgacgcg cagcggtaag gaaaggtgcg atgattgcgt tatgcggaca
     5941 ctgccatccg gtgggaagtc atcatctctt agtatcttag atacccctcc aagcccgaag
     6001 acctgccggc caacgtcgca tctggttctc atcatcgcgt aatattgatg aaacctgcgg
     6061 catccttctt ctattgtgga tgctttacaa tgattaaaaa agcttcgctg ctgacggcgt
     6121 gttccgtcac ggcattttcc gcttgggcac aggataccag cccggatact ctcgtcgtta
     6181 ctgctatccg ttttgaacag ccgcgcagca ctgtgcttgc accaaccacc gttgtgaccc
     6241 gtcaggatat cgaccgctgg cagtcgacct cggtcaatga tgtgctgcgc cgtcttccgg
     6301 gcgtcgatat cacccaaaac ggcggttcag gtcagctctc atctattttt attcgcggta
     6361 caaatgccag tcatgtgctg gtgttaattg atggcgtccg cctgaatctg gcgggggtga
     6421 gtggttctgc cgaccttagc cagttcccta ttgcgcttgt ccagcgtatt gaatatatcc
     6481 gtggaccgcg ctccgctgtt tatggttccg atgcaatagg cggggtggtg aatatcatca
     6541 cgacgcgcga tgaacccgga acggaaattt cagcagggtg gggaagcaat agttatcaga
     6601 actatgatgt ctctacgcag caacaactgg gggataaaac acgggtaacg ctgttgggcg
     6661 attatgccca tactcatggt tatgatgttg ttgcctatgg taataccgga acgcaagcgc
     6721 agacagataa cgatggtttt ttaagtaaaa cgctttatgg ggcgctggag cataacttta
     6781 ctgatgcctg gagcggcttt gttcgcggct atggctatga taaccgtacc aattatgacg
     6841 cgtattattc tcccggttca ccgttgctcg atacccgtaa actctatagc caaagttggg
     6901 acgccgggct gcgctataac ggcgaactga ttaaatcaca actcattacc agctatagcc
     6961 atagcaaaga ttacaactac gatccccatt atggtcgtta tgattcgtcg gcgacgctcg
     7021 atgagatgaa gcaatacacc gtccagtggg caaacaatgt catcgttggt cacggtagta
     7081 ttggtgcggg tgtcgactgg cagaaacaga ctacgacgcc gggtacaggt tatgttgagg
     7141 atggatatga tcaacgtaat accggcatct atctgaccgg gctgcaacaa gtcggcgatt
     7201 ttacctttga aggcgcagca cgcagcgacg ataactcaca gtttggtcgt catggaacct
     7261 ggcaaaccag cgccggttgg gaattcatcg aaggttatcg cttcattgct tcctacggga
     7321 catcttataa ggcactaaat ctggggcaac tgtatggctt ctacggaaat ccgaatctgg
     7381 acccggagaa aagcaaacag tgggaaggcg cgtttgaagg cttaaccgct ggggtgaact
     7441 ggcgtatttc cggatatcgt aacgatgtca gtgacttgat cgattatgat gatcacaccc
     7501 tgaaatatta caacgaaggg aaagcgcgga ttaagggtgt cgaggcgaca gccaattttg
     7561 ataccggacc actgacgcat actgtgagtt atgattatgt cgatgcgcgc aatgcgatta
     7621 ccgacacgcc gttgttacgc cgtgctaaac agcaggtgaa ataccagctc gactggcagt
     7681 tgtatgactt cgactggggt attacttatc agtatttagg cactcgctat gataaggatt
     7741 actcatctta tccttatcaa accgttaaaa tgggcggtgt gagcttgtgg gatcttgcgg
     7801 ttgcgtatcc ggtcacctct cacctgacag ttcgtggtaa aatagccaac ctgttcgaca
     7861 aagattatga gacagtctat ggctaccaaa ctgcaggacg ggaatacacc ttgtctggca
     7921 gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg actccggcgt cggtgggttg
     7981 tcggtctatg acgagatccg gcatctctta ccggatctcc attacattta tgctttcgat
     8041 aacgtcgctt tcccgtatgg cgaaaaaagc gaagcgttta ttgttgagcg agtggtggca
     8101 attgtcaccg cggtgcaaga acgttatccc cttgcgctgg ctgtggtcgc ttgcaacacc
     8161 gccagtaccg tttcacttcc tgcattacgc gaaaagttcg acttcccggt tgttggtgtc
     8221 gtgccggcga ttaaacctgc tgcacgtctg acggcaaatg gcattgtcgg attactggca
     8281 acccgcggaa cagttaaacg ttcttatact catgagctga tcgcgcgttt cgctaatgaa
     8341 tgccagatag aaatgctggg ctcggcagag atggttgagt tggctgaagc gaagctgcat
     8401 ggcgaagatg tttctctgga tgcactaaaa cgtatcctac gcccgtggtt aagaatgaaa
     8461 gagccgccag ataccgttgt attgggttgc acccatttcc ctctactaca agaagaactg
     8521 ttacaagtgc tgccagaggg aacccggctg gtggattctg gcgcagcgat tgctcgccga
     8581 acggcctggt tgttagaaca tgaagccccg gatgcaaaat ctgccgatgc gaatattgcc
     8641 ttttgtatgg caatgacgcc agaagctgaa caattattgc ccgttttaca gcgttacggc
     8701 ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg gttgaatgtt gcgcggtcgg
     8761 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
     8821 gacacggaac aacggcaaac acgccgacgg gtcagcgggg ttctcctgag aattccggca
     8881 gagaaagcaa aaataaatgc ttgactctgt agcgggaaag cgtattatgt acaccccgcg
     8941 cagctgagaa aaagcgaagc
//
LOCUS       DAJTRW010000137         8886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRW010000137 DAJTRW010000000
VERSION     DAJTRW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..248)
                     /locus_tag="OO326_003613"
     CDS             complement(<1..248)
                     /locus_tag="OO326_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5967862.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="OO326_003614"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="OO326_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="OO326_003615"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="OO326_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="OO326_003616"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="OO326_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO326_003617"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO326_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5967863.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="OO326_003618"
     CDS             complement(2964..3278)
                     /locus_tag="OO326_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967864.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="OO326_003619"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="OO326_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO326_003620"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO326_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5967865.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="OO326_003621"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="OO326_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="OO326_003622"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="OO326_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5945..6104)
                     /locus_tag="OO326_003623"
                     /pseudo
     CDS             complement(5945..6104)
                     /locus_tag="OO326_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6108..7337)
                     /locus_tag="OO326_003624"
                     /pseudo
     CDS             complement(6108..7337)
                     /locus_tag="OO326_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO326_003625"
     CDS             complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO326_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5967866.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7704..8078)
                     /locus_tag="OO326_003626"
                     /pseudo
     CDS             complement(7704..8078)
                     /locus_tag="OO326_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8148..>8886
                     /locus_tag="OO326_003627"
     CDS             8148..>8886
                     /locus_tag="OO326_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5967867.1"
                     /translation="MFPDSFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRHWMIPLRKGAQY"
BASE COUNT         2250 a         2117 c         1988 g         2531 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca
     5761 tttcatactg aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg
     5821 ggccctaatt aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg
     5881 caagcagaca catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt
     5941 aagtgaggct ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc
     6001 gccagcgatt gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat
     6061 ggataatagt gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc
     6121 tgtatccagt catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga
     6181 cgctgttctg ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc
     6241 tgcagggctt ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc
     6301 cggacatgag ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa
     6361 ccaccgtaag catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg
     6421 atacctttcc ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg
     6481 ttacggtcat cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc
     6541 tcctggaccg gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg
     6601 cgcaggatgt catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt
     6661 gtggcacggc tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc
     6721 agatagtctg catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg
     6781 cgtgcaatgg gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc
     6841 agtttcggac gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct
     6901 gacacatcac atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc
     6961 ggacggatga cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac
     7021 tggcgcaggg gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct
     7081 tcctgaagtg cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga
     7141 agtttttctg agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc
     7201 cggttgattt cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc
     7261 atctggcgga tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga
     7321 gcaagtgagg tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc
     7381 cgttcgtttc gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt
     7441 aaggtgcacc ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct
     7501 ggtaaagagg cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg
     7561 tccgcggaag atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt
     7621 tgagaccagg ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg
     7681 cgaacctgca gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc
     7741 tgtaataacg ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc
     7801 tcacagcaca gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg
     7861 attttattat ccggctccgg cgttaacgtc acgggaagca gtgcaggcat attttttccg
     7921 gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag gttatcgttg
     7981 attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga ctgctccact
     8041 aaagcgattt taaattcata aggaaaatta ggccgccggt gtaatgccga tcagttaagg
     8101 atcagttgac cgatccagtg gctgtgtaag aatccggaaa cgctcacttg tttccggatt
     8161 cttttatgca cattggacag gctcttgatc tggtatcccg ttacgattct ctgcgtaacc
     8221 cactgacttc tctgggggat tacctcgacc ccgaactcat ctctcgttgc cttgccgaat
     8281 caggtactgt aacgctacgc aagcgccgtc ttcccctcga aatgatggtc tggtgtattg
     8341 ttggcatggc gcttgagcgt aaagaacctc ttcaccagat tgtgaatcgc ctggacatca
     8401 tgctgccggg caatcgcccc ttcgttgccc ccagtgccgt tattcaggcc cgccagcgcc
     8461 tgggaagtga ggctgtccgc cgcgtgttca cgaaaacagc gcagctctgg cataacgcca
     8521 cgccgcatcc gcactggtgc ggcctgaccc tgctggccat cgatggtgtg ttctggcgca
     8581 caccggatac accagagaac gatgcagcct tcccccgcca gacacatgcc gggaacccgg
     8641 cgctctaccc gcaggtcaaa atggtctgcc agatggaact gaccagccat ctgctgacgg
     8701 ctgcagcctt cggcacgatg aagaacagcg aaaatgagct tgctgagcaa cttatagaac
     8761 aaaccggcga taacactctg acgttaatgg ataaaggtta ttactcactg ggactgttaa
     8821 atggctggag cctggcggga gaacaccgcc actggatgat acctctcaga aagggagcgc
     8881 aatatg
//
LOCUS       DAJTRW010000138         8749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRW010000138 DAJTRW010000000
VERSION     DAJTRW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            7..438
                     /locus_tag="OO326_003628"
     CDS             7..438
                     /locus_tag="OO326_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5967868.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO326_003629"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO326_003629"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5967869.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO326_003630"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO326_003630"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5967870.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO326_003631"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO326_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5967871.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OO326_003632"
     CDS             complement(4440..5147)
                     /locus_tag="OO326_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5967872.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO326_003633"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO326_003633"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5967873.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OO326_003634"
     CDS             complement(6005..6208)
                     /locus_tag="OO326_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5967874.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7875
                     /locus_tag="OO326_003635"
                     /pseudo
     CDS             6397..7875
                     /locus_tag="OO326_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8749
                     /locus_tag="OO326_003636"
     CDS             7872..>8749
                     /locus_tag="OO326_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5967875.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPG"
BASE COUNT         2029 a         2386 c         2249 g         2085 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccagg
//
LOCUS       DAJTRW010000139         8721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRW010000139 DAJTRW010000000
VERSION     DAJTRW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..690)
                     /locus_tag="OO326_003637"
                     /pseudo
     CDS             complement(1..690)
                     /locus_tag="OO326_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(741..1316)
                     /locus_tag="OO326_003638"
                     /pseudo
     CDS             complement(741..1316)
                     /locus_tag="OO326_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1320..1720
                     /locus_tag="OO326_003639"
                     /pseudo
     CDS             1320..1720
                     /locus_tag="OO326_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2123..2704)
                     /locus_tag="OO326_003640"
     CDS             complement(2123..2704)
                     /locus_tag="OO326_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967876.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2751..6608)
                     /gene="sigA"
                     /locus_tag="OO326_003641"
     CDS             complement(2751..6608)
                     /gene="sigA"
                     /locus_tag="OO326_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5967877.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(6969..7973)
                     /locus_tag="OO326_003642"
                     /pseudo
     CDS             complement(6969..7973)
                     /locus_tag="OO326_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            7958..8335
                     /locus_tag="OO326_003643"
                     /pseudo
     CDS             7958..8335
                     /locus_tag="OO326_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8374..>8721)
                     /locus_tag="OO326_003644"
     CDS             complement(8374..>8721)
                     /locus_tag="OO326_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5967878.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2109 a         2072 c         2019 g         2521 t
ORIGIN      
        1 actgcagtac acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt
       61 atccgagtga cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat
      121 cagtggcgcg gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac
      181 ttctgcctcg atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt
      241 tggcactctt gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc
      301 cgttattctg ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac
      361 accgctgtaa ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc
      421 gaaccagacc gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca
      481 cagccgggca gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc
      541 cgcatgaact ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag
      601 cagttcagca acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg
      661 acgacggtat atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc
      721 cgcaacctgg gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca
      781 aatcctgctc gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct
      841 tcgacaacac cattactcca gcgactactg attgcctcac atatagctgc agcatcagct
      901 tccatccccg ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac
      961 caactgctta gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt
     1021 tgctgcgcta ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca
     1081 taattttctt catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact
     1141 gaaggaagtc tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc
     1201 gcatccctga cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc
     1261 cggctggcgt tataattacc actttctcgt tgctctttta accactccct ccatggtgtc
     1321 agcgaaaaac gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac
     1381 ccagtcgcga cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc
     1441 actgacgtac tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc
     1501 cttgcgggcg gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg
     1561 tgagcgggga gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc
     1621 agaaagctac cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag
     1681 cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc
     1741 cctcgctgta cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg
     1801 acgcgatcac cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc
     1861 ctgtgcaaca aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg
     1921 acgcgcgcct gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat
     1981 gaacattaca acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc
     2041 gaatgaccgg cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg
     2101 ggctaaaata ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa
     2161 gtactgagct tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc
     2221 cctgaaataa tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca
     2281 tatttttaat gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac
     2341 tgagtctgat gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt
     2401 cagtgaccag catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg
     2461 tgattgtaat gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc
     2521 cgtatcggaa ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt
     2581 tttgcctgat agtttctttt tttattcgct catgtttaag gcataacagg cagataaata
     2641 atgtcagaaa aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat
     2701 acatattctc tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag
     2761 taacggaagt tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca
     2821 aactcaagac cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga
     2881 atacgaccgt ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt
     2941 tcaccgttgg caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag
     3001 tccttaccgg agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag
     3061 tccttatcct tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat
     3121 acggccccat aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag
     3181 cggtagcctg cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt
     3241 ccagcaaagg tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata
     3301 taggcaccag agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg
     3361 ccactgaatg cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa
     3421 aggtcaagtc catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg
     3481 tcactgtatc caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc
     3541 tcgccattgg tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg
     3601 aaggctttgt aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca
     3661 gccaccttat atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc
     3721 acaggagata tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg
     3781 cttccttttg gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc
     3841 tgtatatcga ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta
     3901 tccgattgat ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca
     3961 tccagtgtgt ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa
     4021 agactgtcgc gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg
     4081 ttatacatgc tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag
     4141 ccacccagca aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta
     4201 tactggtcaa caccaaacag aatagatgct tttttgtctg attttatatt accattgacc
     4261 caggcataat tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac
     4321 atatagattg ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt
     4381 ccggccagtt caatagcttt atctacatac atttttccct gggcggtaac agtagctcct
     4441 ttgttgataa tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat
     4501 ttatcaccaa cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg
     4561 cctgctgaat ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt
     4621 cttcctgaga acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta
     4681 gcctctatgt gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa
     4741 tttaatttcc ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc
     4801 tgattgttgg acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt
     4861 atggcagcag aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct
     4921 ttaaatatgg catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag
     4981 atatcgtttt tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc
     5041 tgggtagtat tgataatatg atttatgctt atattacctg atacattccc atggtacata
     5101 tatgcatcag tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt
     5161 gttcccgcat ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt
     5221 gttccgccat ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg
     5281 gcatttattt ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca
     5341 gcactaagga tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca
     5401 tccagagtac cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat
     5461 tcaacggtgg tattattatc aatatctatc ccggcacctt tataagactt atcttttccg
     5521 tacactttat atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc
     5581 tgcttgagcg ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat
     5641 gataactctt gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg
     5701 tttaccagaa tattattaaa taacgagtag tttgtccagg ctatattggt actactgata
     5761 aaaccaactg tactggtaac accatggaaa acccattttt gttgttcttt atcatataga
     5821 taagaaccag aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta
     5881 taaatgtaac tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga
     5941 ttatagtttt gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca
     6001 cctgctctga agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc
     6061 gcatcttcgg gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg
     6121 aatttattca gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat
     6181 gctgtctgat cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca
     6241 gtaacactgt acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa
     6301 tcaggtacag gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca
     6361 tttgttgcac ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc
     6421 catatattat ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt
     6481 gaggataaaa taataccgag gataactttc tttcttgatg taccgacact aactttccgg
     6541 gtcagttcag aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt
     6601 ttattcatac gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca
     6661 acggggaact atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt
     6721 tatatttgta tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg
     6781 ttagatattg atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt
     6841 tgtttccggt caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat
     6901 gtccgacaga cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca
     6961 ggtggaactc agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg
     7021 atcaggcctg caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg
     7081 aatgcactgc cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat
     7141 ttccaggcat taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa
     7201 ccgctggccg gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag
     7261 acaccggccg tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt
     7321 gtctcaatac tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc
     7381 aggtccgtac tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg
     7441 cctttgaagg cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg
     7501 cgctggaatt tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta
     7561 gccagagcaa tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg
     7621 cggcttttgt ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca
     7681 gcatcagaga ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca
     7741 gagaggcgat caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt
     7801 gtgttacgga agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg
     7861 ttactttcga tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg
     7921 gtgatattat ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca
     7981 tgcgcgacgc aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc
     8041 tctgaaacta atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc
     8101 tgaacgcctg gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct
     8161 gatgcaggag aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta
     8221 tatcaggaag cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga
     8281 taataatgca gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag
     8341 gatcactcat tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga
     8401 tgattgggca gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt
     8461 aatggctact tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg
     8521 ttgttgagct ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata
     8581 tataagccat gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag
     8641 acgtctcagt tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact
     8701 ggaatattca cctggcacca g
//
LOCUS       DAJTRW010000140         8681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRW010000140 DAJTRW010000000
VERSION     DAJTRW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO326_003645"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO326_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5967879.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO326_003646"
     CDS             1828..3147
                     /locus_tag="OO326_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5967880.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO326_003647"
     CDS             3445..3486
                     /locus_tag="OO326_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967881.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO326_003648"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO326_003648"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5967882.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO326_003649"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO326_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO326_003650"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO326_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO326_003651"
     CDS             5815..6711
                     /locus_tag="OO326_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5967883.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO326_003652"
     CDS             6708..7682
                     /locus_tag="OO326_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5967884.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO326_003653"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO326_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2118 a         2116 c         2195 g         2252 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag c
//
LOCUS       DAJTRW010000141         8643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRW010000141 DAJTRW010000000
VERSION     DAJTRW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO326_003654"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO326_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5967885.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="OO326_003655"
     CDS             complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="OO326_003655"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5967886.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="OO326_003656"
     CDS             complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="OO326_003656"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5967887.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="OO326_003657"
     CDS             complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="OO326_003657"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5967888.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5267..7813
                     /gene="cyaA"
                     /locus_tag="OO326_003658"
     CDS             5267..7813
                     /gene="cyaA"
                     /locus_tag="OO326_003658"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5967889.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="OO326_003659"
     CDS             complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="OO326_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5967890.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8221..>8643)
                     /locus_tag="OO326_003660"
     CDS             complement(8221..>8643)
                     /locus_tag="OO326_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5967891.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2235 c         2224 g         2217 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     2101 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     2161 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     2221 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     2281 ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2341 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2401 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2461 gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt
     2521 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2581 gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2641 aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac ttttcagcaa
     2701 cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2761 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgatctt
     2821 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2881 ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2941 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     3001 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     3061 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     3121 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     3181 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     3241 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3301 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3361 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3421 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3481 aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac gaccgccatt
     3541 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3601 gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta cggtatgtag
     3661 cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc atttacgatc
     3721 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3781 atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3841 aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac ggctcactaa
     3901 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc ctctccgtta
     3961 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     4021 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     4081 tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc ggcgtagcta
     4141 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     4201 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca
     4261 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     4321 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4381 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4441 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4501 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4561 gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg cgggaattca
     4621 accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag gagcgcgact
     4681 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4741 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4801 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4861 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4921 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4981 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     5041 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     5101 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     5161 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     5221 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     5281 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5341 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5401 ccatccgcga atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5461 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5521 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5581 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5641 cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa gctgggccgc
     5701 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5761 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5821 attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5881 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5941 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     6001 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tgctgaaaac
     6061 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     6121 caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat actgcatgat
     6181 gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc tggatttagt
     6241 acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg
     6301 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6361 ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6421 gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6481 ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc
     6541 gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6601 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt caggttggta
     6661 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6721 ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6781 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6841 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6901 cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc cgacagctgc
     6961 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     7021 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     7081 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     7141 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     7201 tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     7261 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7321 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7381 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7441 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7501 tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7561 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7621 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7681 gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc cgttccgaac
     7741 aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc tattacagca
     7801 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7861 aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7921 cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt
     7981 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     8041 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     8101 ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     8161 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     8221 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     8281 aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac gagttttcgc
     8341 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8401 gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa tttgcaagct
     8461 ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa atgagcgatc
     8521 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8581 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8641 ttg
//
LOCUS       DAJTRW010000142         8521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRW010000142 DAJTRW010000000
VERSION     DAJTRW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO326_003661"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO326_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="OO326_003662"
     CDS             complement(998..2020)
                     /locus_tag="OO326_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5967892.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="OO326_003663"
     CDS             complement(2034..3536)
                     /locus_tag="OO326_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5967893.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OO326_003664"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OO326_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="OO326_003665"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="OO326_003665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5967894.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5852..6193)
                     /locus_tag="OO326_003666"
     CDS             complement(5852..6193)
                     /locus_tag="OO326_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5967895.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6196..>8521)
                     /gene="tamB"
                     /locus_tag="OO326_003667"
     CDS             complement(6196..>8521)
                     /gene="tamB"
                     /locus_tag="OO326_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5967896.1"
                     /translation="PELKLTGNVKQNKVNVDGTLKGNSYMQWMIPGLHLELGPNSAEV
                     KGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGTVEAPQLLADITARGLRWQ
                     ELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLVTLNAKGSEKQHELQLRIQ
                     GEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPWSLTRDIALDYRNKEQKISIGP
                     HCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLKPFMPETTQASGIFTGKADVAW
                     DTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLTAELRNNRAELGWTIRLTN
                     NGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIFTRGEKAAGMVSANLRLGGDVQ
                     SPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTLAGTVRTQQGEIYLNGDAD
                     WSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATPNLFTLDGRVDVPWARIVV
                     HDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVHVGNNVRIDAFGLKARLTG
                     DLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLFSGPPDQPYLNIEAIRNPD
                     ATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLRGQGLESDQSDSAAMTSML
                     IGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQVVVSGYVLPGLQVKYGVGIFDS
                     IATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
BASE COUNT         2008 a         2410 c         2150 g         1953 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtaatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaaatcg cgagatacgt cgcataaaac
     5821 aacgccaccc gaaggaggcg tgcttttatt attacttatc cctgtctaac cagtcgccgc
     5881 tttcaattag ctttaatcca tcgacgggtc gttgataaac gtacatccat gcactcccat
     5941 acggcgtctg aatcaactgg cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt
     6001 cggccagcgt ggcgttgtca atacgataaa cttcaccgtg taccgttccg ttccccggaa
     6061 ctgcgcctgg atagtggccc aggctataca actggtagtt atcgatactg aaatcgccca
     6121 gtaactgggc attggtcatc cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga
     6181 caaatattcg cattgctaaa actcgaactg atagagcaaa tccagtgcct ggtctacacc
     6241 agacacggct tccagatata gcttaggcat caggcgataa cgtaacgtga gtgttgctat
     6301 agagtcaaat atacccacgc cgtatttcac ttgcagacct ggcaatacat agccgctgac
     6361 cactacctgg gaggagtcgc ctactcccag ggtgtcgagc gctaaattgc ttacgccaaa
     6421 cgtctcgccg attttaccca caatctggcc actttgcgca acccccagac caatcagcat
     6481 cgaggtcatt gccgcactgt cgctctgatc gctctccagc ccttgtccac gtagcaaata
     6541 agacaatgca gcttgttgcg acatcgccgg gtcagagaag atctccgctt tcggttcgtc
     6601 cgccagacca gtgacgcgaa ctccggcgat tacatcgtct tctgtagcat ccgggttacg
     6661 aatagcttca atattaagat acggttgatc cggcggacca gagaacagta actcaccctt
     6721 acgcacaatc agatcctgac cataggcatg gaagcgccct tcagggatgt tgatctgccc
     6781 gttcaggccc agcccttgtt tgtcctgtac gacgttaaga tcgcccgtca gccgcgcttt
     6841 caggccaaag gcgtcaatgc gcacattgtt gccaacgtgg acaatcaggt tactgttaat
     6901 cggaatcgac gccgttttcg gctcttccgg ttgcaggtta tcgttaagca tcaccacatc
     6961 gctggagacg cctactgcgc tttccggcag atcgtgcacc acgatgcgcg cccacgggac
     7021 atccacgcga ccatcgaggg taaacaggtt tggtgtagcc tcgaatacaa catctggcga
     7081 tacatccatt cgtaccatcg gcggcacggt gatccgcact ttactgcctt tcgccgttac
     7141 tcgtgcccgc cagttttcaa tttggctcca gtcggcatca ccgttcaggt agatttcacc
     7201 ctgctgggtc cgtactgtac cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag
     7261 ctggctcggc tgcatatcaa acggcataaa gttgccgtcg atatccacac ccgtaacctg
     7321 aagctgacca aacaactgcg ggctttgcac atcaccaccc agacgcaagt tggcactcac
     7381 catccccgct gctttttccc cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt
     7441 acggatattg acgttgccac caagattacg gcggccttgc ggatcggtca cctgcacctg
     7501 tccatcaaac tggccgttat tggtcaggcg gatggtccag cccaattcgg cacggttgtt
     7561 acgcaattcc gccgtcagat tcagtgtctg aaacgccacc ggcagcgccg catcgttgac
     7621 ggtttgcgtt acctgcacgt tacgccccga aagggtgata ctgccctgcg gcagcccctc
     7681 tttcgtggtg tcccaggcaa catccgcttt acccgtgaag ataccgctgg cctgagtggt
     7741 ttctggcata aacggtttca gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg
     7801 cccttcggcc cccgcatcga tagtttgcgg cacgcacagt tccgcattcg ggttaagcca
     7861 gcagtgtggc ccgatgctga ttttttgctc cttattgcgg taatccagcg caatatcgcg
     7921 ggtcagcgac cacgggccga ccggcgtctg gaagcgggta ttgctaagag ttcccttcca
     7981 gcgttcttct ttgcgatcaa aacttcctgc cagattaagc tgcccggaga caggctcgcc
     8041 ctgaatccgc aactgtagct cgtgctgctt ttcgctgcct ttggcattca gggtgacgag
     8101 gttgatattt acatccggct gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat
     8161 ctgatcggtg gatttaatgt cgccttccac gcgaacctgc gccacggaaa gttcctgcca
     8221 gcgcaggccg cgcgcggtga tatctgccag tagttgtggc gcttccaccg tgccgcgtac
     8281 tttcaccagc cctttcgctg taccgccaag ccccggcagc gcgttatcca gccccggcgc
     8341 gttgatggtg gcatcaagat tgagatcttt tacccccagc tcgcctttca cttcggcact
     8401 gtttggcccg agttccagat gaagcccagg gatcatccac tgcatataac tgttgccttt
     8461 cagcgtgccg tcaacgttca ctttgttctg tttgacgtta ccggtcagct tcagttctgg
     8521 c
//
LOCUS       DAJTRW010000143         8473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRW010000143 DAJTRW010000000
VERSION     DAJTRW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..81)
                     /locus_tag="OO326_003668"
                     /pseudo
     CDS             complement(1..81)
                     /locus_tag="OO326_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            79..403
                     /locus_tag="OO326_003669"
                     /pseudo
     CDS             79..403
                     /locus_tag="OO326_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(433..3345)
                     /locus_tag="OO326_003670"
     CDS             complement(433..3345)
                     /locus_tag="OO326_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967897.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3348..3464
                     /locus_tag="OO326_003671"
     CDS             3348..3464
                     /locus_tag="OO326_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5967898.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3828..4670
                     /locus_tag="OO326_003672"
     CDS             3828..4670
                     /locus_tag="OO326_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5967899.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4673..5761
                     /gene="capU"
                     /locus_tag="OO326_003673"
     CDS             4673..5761
                     /gene="capU"
                     /locus_tag="OO326_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5967900.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5766..6716
                     /gene="virK"
                     /locus_tag="OO326_003674"
     CDS             5766..6716
                     /gene="virK"
                     /locus_tag="OO326_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5967901.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6781..7725
                     /gene="lpxM"
                     /locus_tag="OO326_003675"
                     /gene_synonym="msbB"
     CDS             6781..7725
                     /gene="lpxM"
                     /locus_tag="OO326_003675"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5967902.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7879..>8473)
                     /locus_tag="OO326_003676"
     CDS             complement(7879..>8473)
                     /locus_tag="OO326_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5967903.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2237 a         1924 c         2009 g         2303 t
ORIGIN      
        1 ctccgacata cctgcaggca aaggccatga aagccctgat ttccggaagc gagcgaacag
       61 tccacaaact gttgaacgtg gtgtcagcgg cggacaggga taagccctcg tcagcagttc
      121 cgtcagcact gcgacagcgt tctttacccg gtcaaaacag cgttgcggtg caccacgcct
      181 gacggatgaa ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct
      241 gcgccgtcag gggctgaggg caaaggcctc ccggaagttc agcccggtca gttaccgcgc
      301 acatggcctg cccgtgtcag aaaatctgct ggagcaggat ttttacgcca gtggtccgaa
      361 ccagaagtgg gcgggagaca tcacgtactt acgtacagat gaagttcggt tacatcctgt
      421 cagcacggaa cctcacgctt tttgagcggc acgacggatc ccccccaccc agtatccgac
      481 agacttttca ctgcgttcgt aaaaaccctt cttccctccc agcatatcca ctaattccgg
      541 tgacatatcc tgcagataag tctgcaaatc atgccccaga tcttccggtg actgcaactc
      601 ataaaccgcg cgccacacat cacccttcct tattttaagc cccgggttaa gaatatactg
      661 tccgtggttc agcctcagaa aaagcttttt tccataagga tttttgctgt taacttcatg
      721 ttgagagagc tggctgctga tataactcct tcgtttgcgg cgttccggca gcaacgcttc
      781 aggaagatga accacacact ctgccagtcg tgccgcactg aaggcttccg aaaaaaacag
      841 agcacttccc agatgcgtgt ggaacagcgc ctgcatcagg atcaccagaa acattgccat
      901 ctgccggttg tccagtttga tgaggcgtcc ttccacctgc agtgacagac tttcaggttt
      961 cagcagggta tacaactgcg cacttttctg ttgcgcataa cggggcgtac tgaccgccgc
     1021 tgacagaact tgatgataag ccgccagccc gtcattaccg gtcagcaagg gatctgcacc
     1081 acgctccagc agtaactgaa ccagtgcaat attacctgcc cgggctgctg acatcagcgg
     1141 agtaagatta aacggcgaac ggtgatcaac accataacgt tcaatatccc gcaacacggc
     1201 aaccgggtta ttcattgagt atggccggaa atacttcagc tccagtaact gcagagtctt
     1261 cgttctgggc tgccttgtgg gcttaaaatc ttcccggtac agtgcattat acagcgggaa
     1321 cagggaataa atcagactgt actccagcgc ctgcagttgt atggttttat cggctgtacc
     1381 tgcgaggata tgctgtttcc actgtcgtgc atcctctgcg gtgataatct gccatggcat
     1441 ctcagatgtc tgcaggatcc ggctacggat ggtgtccgcc tgctcctgct tcccctgttt
     1501 ttcaagcaga tgtgcggttt tctgccactc atcacggctg gaaatctctt cctgcagatt
     1561 cagaggctct tcctgatgcg tgagcgcaag cagactccac agaggatgct ccacctgctg
     1621 ttcaacaaga tacacattgt gtttagcacg ggtgagcgca acatacagcg cgttggtaaa
     1681 aaatttatag atttccgctg aacgatcctg cctgtcccgc ggacgggaat aacgggcctc
     1741 gccctcaaga tcagccggtg taagaccctc gcaaatatca tcaaatgcct gccgggcagc
     1801 gcttactata ttgtaaagga tcaccgtttc gtattccagc cccttaacct cctgaaccga
     1861 aaagaccagc ggcgtactga accagcatcg cgcctgtgct ttctgttccg gatgcatcac
     1921 aatgaccgct acacgattcg aaagtgaggt tttggcattc agttcctgct ttgtctcttc
     1981 cctatcgtcc agcaaccgga tggtcccttc tgcctgaccg cagcttctga caaaatggtg
     2041 actttcccga tcaatagcac taaagcggac ctgtttgagt ctcagcaacc ggttagccag
     2101 tgcagtgacg tggtgcccgt tacggtaatt cgcctgcagg atattaacgg ttgtgtcgtt
     2161 tccctgttgc tgccggaaaa agagactttt gagcgaactc catgagaaaa agctcggatg
     2221 aactatctga ttggcatcac cacagagtaa aaactgaccg ggatgacgta gcgttttcag
     2281 taccagttgc agttgaacag gcgtcatatc ctgaacttca tcaacaaaaa tgacatcata
     2341 acggggagtt gccaacgaaa gatactgatg actgagcaga ttactgtcat actgatgcga
     2401 ctgtttcagc catgcgatat aacgttcaaa caggacatag accgtcgggc ggtcctccat
     2461 gccatagagc gactgccgta tccccagtga cagatacgcc tcccgggaca gcggcccatt
     2521 cccggaggca accgcaccga tcacgcccct gaactcctca tacagggtat gggcagcccc
     2581 cagtgcggcg atagccctgt tgcggggcag ccagtcgctg aacgcactga aagtcacctc
     2641 cctgccttcc ggaatgcgca gagtttccag aaattccgtc agcgaaagaa agtcaatatt
     2701 ctgtacctca ctcccttccc ctgaagcgtc atagagagta cgcgcttttt ccaccagaaa
     2761 tgaggaaagg ctgagataca gtatatcacc agcagcctgc ttcatttttt ccagtaccag
     2821 cgccgtctta ccacttcccg ccgagccaac aatcaccagc ggtagcgggt gttcgtacag
     2881 tgcctgctga tcatcatcaa aggacagcat tttatccagg cggtggaaac gcccatgaga
     2941 cggattgata tacgtcagcg cttcagtcgc gatatcaaca ggatccggca ctggttgttg
     3001 ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag tgttataggc
     3061 atgattgcga atatattcca gaacaaggca gatagtttca ccgtggtagc gatacagcga
     3121 aaacagtaat cggtcccgga tactgagccg ggcgcggtaa agattggtgt caattttacg
     3181 aacatcagcc tgaacaaagt tatcggtggc aatagcctgt gcaaattttt tataacccgg
     3241 gatcgtgtca ggattcagag tatgataaag acagattttc atggctgccg gtttccgtcg
     3301 tgcaaaaaac atgctgattt tacgaaggaa tgccttactg aacaaccttg cccggaatac
     3361 cccccgaaat cagatggaac aatcagaaaa atgctctgca gttatggaat aaagaaggtg
     3421 gggtggtacg ccgggagggc gatccgctgg aaatggcgca gtagctgcag gcctgtcatg
     3481 atgcaggaat agaagttcgt gtacaggtta atgaaagcgt caccccgtaa ataaactctg
     3541 cgctgaaatt cctttttttc attggagtac ctgtgttgtt ctgaggtgaa catatcacct
     3601 ctgttcaggt agccaaattc agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa
     3661 gatatgataa tatatcattg tttatgaatt gttgatgttt aatttagtaa attatatcaa
     3721 atatgccatg ggtttgctat gttattcatc cagtatctgg aaataaaatg caccagcttt
     3781 attgggtttt ccttttgtct ctgcttgcta ggtgccagaa actttaaatg ttaaacgaag
     3841 ggggcatttt gttcaaagcc aatcatgttc cggtattaat gtatcatcat gtcagtcatt
     3901 gccccggact ggttacactt tctcccgtga cattccggaa gcaaataaaa tggttggctg
     3961 aaaacaactg gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac
     4021 tcccccggaa aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg
     4081 tatatccttt attaaaagag tttaatctca aagcacatat ttttttaatt accggtttta
     4141 ttgggaatgg tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac
     4201 atcaaatagc gacagggaat gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa
     4261 tgttgcaatc tggtctggtc gaatttcatg ttcatacgca tactcataca cgctgggata
     4321 aaaagttcag ttcacgagaa gaacagtgta aacatcttcg gcaggacttg ttgagcggga
     4381 gggaatattt gaaagaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt
     4441 attataacaa ggattatatt caggtcgctg aagaacttgg attctattat ttatatacaa
     4501 cagaaagaag aatgaatgct cctgctaaag gaactacacg gataggaaga ataagcacca
     4561 aagaacggga aagttgcgca tggctgaaaa gacgactttt ttattataca accccgtttt
     4621 tttcatcgtt gctggccttt cataaggggc caagattacc ggatgattaa taatgaatat
     4681 actatttacg gaatcatcac cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat
     4741 gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat
     4801 tgcttttgaa gccagtaaat tggggattga tatcacattc gcgttatttc gaaacagtct
     4861 tcacatccct actgcatgga gattactcgg aatagttcat ggttttcagc ccaatgcaat
     4921 cgtttgtcac agtgggcatg atagcaatat tgttggttta gtacggttat tcactaggaa
     4981 acatccattc agaattatca ggcaaaagac atatttgaca cgaaaaacaa aagttttttc
     5041 aataaatcat ttttgcgatg aggtgattgt tcccggaaca agtatgaaga cacatctgga
     5101 gcaggaagga tgtcgaaccc gggttactgt tgtgcctcca ggctttgatt tccagaaatt
     5161 atacgttgat tcccgaaaca gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag
     5221 gggatgcccc gttattgctc aggtaggaat gttgcgcccg gaaaaagggc acgaatttat
     5281 gttgaattta ctgttccatt taaaaatgaa tggacgacag ttctgttggt tgattgtggg
     5341 gtctggttcg cctgaactgc gggagcattt acagtatcag attgacagta tgggcatgca
     5401 tgatgatgtt tttattgctg acaatgtttt tcctgccgcc cccgtatatc gggttgccag
     5461 tctggtggtt ctgccttcag aaaacgaatc gtttggtatg gtactggcag aagcatcggc
     5521 attttctgtg cctgtactgg ccagtcagat tggtggaatc cctgatgtta ttcagaataa
     5581 ccagaccggg acattgttac cagcaggtaa taagcacgca tggatgtgcg ccctgaatga
     5641 tttttttaat gaccctgggc gtttttatca gatggctcgc caggcaaaac aggatataga
     5701 agagcggttt gatattaata aaactgcgtt aaaaatactc acattagcga agcacaagta
     5761 acaatatgtt ttctgtaagt aacttatcat ttatcggttt ccttaaaagg attgtttttt
     5821 cctcagattc actcccgggg aagtgggaac acagaaaatt ccggttcatg tacattttgc
     5881 gatgtgctat aaatccggtt gccagtattc gatattatta cgaactgcgt tccttgcagt
     5941 gcattgagga tattctggca atacaaccca cgttgccagc aagaattcat cgaccttatt
     6001 tgcataaagg ggggcgagcc tggtcccggg ggcaatatat tcttgagcat tatcgctttg
     6061 tgcaaaattt gccagaaaaa tattctgaat ttttgtttcc acaaaaatcg gtatcattag
     6121 tccaattcat cgggaaagat ggagaggatt ttgatatcca gtgttctccc agtggttttg
     6181 accgggaagg agagttgatg ttgtcattgt ttttcaataa aatagtgatt gcccggttaa
     6241 cattttcagt cattctgact caaaatggac acaccgcttt tattggcgga ttacagggag
     6301 cccccaaaaa taccggacct gatattattc ggtgcgcaac ccgggcctgt tatgggcttt
     6361 tcccaaaacg gattattttt gaagcatttt gtgcgttgat gaaagcttgt aatgtctctg
     6421 agtgtctggc agtcagtgag cacagtcatg ttttccggca attgagatac tggtatcaga
     6481 aacgcaaaac ctttgttgct gtctacagtg atttctggga gtctgtagca gggaaaacct
     6541 gtggtgactg gtacaaatta ccaacacagg tggttcgaaa gccgctaagc aatatagcta
     6601 gtaaaaaacg ctctgagtat cgaaaacgat acgctttgct ggattatatc catgaaactg
     6661 ctatccgctc tttggatgca tatcctgtaa actcagaaca tcaggactta aattaaggtt
     6721 tgatgtattc tctgaataaa atattaatga tgattttggt aggggcattc gcactaaata
     6781 atgaaaaaat acaaatccga gtttattcct gaatttaaga aaaattatct ttcccctgtt
     6841 tactggttta catggttcgt tttgggaatg attgcaggta tttcaatgtt tcccccttca
     6901 ttcagagatc ctgtcttggc caaaataggg cgttgggtgg gcagattgag cagaaaagct
     6961 cgtcgcaggg cgacgattaa tttatcgctt tgtttcccgg aaaagagtga tacagaacgg
     7021 gaaataattg tcgacaatat gtttgccaca gcattgcaat ccatagtgat gatggcagaa
     7081 ctggcaattc gtggtccgga aaagttccag aaacgagtgt tctggaaagg cctcgaaatt
     7141 cttgaggaaa ttcggcacaa taacagaaat gtgatttttc tggttcccca tggctggagc
     7201 gtggatattc ctgcaatgtt gctggcagct cagggggaaa aaatggccgc catgtttcat
     7261 cagcaacgaa atccagtgat tgattatgtc tggaattctg tacggcgtaa attcgggggg
     7321 cgcttacatt cccgggagga tgggataaaa ccatttattc agtcagtacg ccagggatac
     7381 tgggggtatt accttccaga tcaggatcat ggtcctgaat acagtgaatt tgctgatttt
     7441 tttgcgacct ataaagcgac attaccaatt attggacgtc tgatgaacat cagtcaggct
     7501 atgattatac cgcttttccc ggtttatgat gaaaaaaaac atttcctgac tattgaagtt
     7561 cggccaccaa tggatgcatg cattgccagc gcggacaata aaatgattgc ccgacaaatg
     7621 aacaaaacag tggaaatttt ggtggggtca catccggaac agtatatctg ggttttaaaa
     7681 ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc cttgaaatta taaagtacag
     7741 gtatttccac tagttgtttc ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt
     7801 tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa
     7861 tagctgcgct taataccgct acacttttgc cagcccatgt ttgcctccgg ggaatgggct
     7921 gacggtttcc ataaaatggc gaactttttt caacagttgc cacattgagc ggcactgatg
     7981 attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt tcaacatgat tcacccatgg
     8041 cgagtaaacc ggctgataaa tgcccctgaa cttcgggttc tccttcagcc agctctgtgt
     8101 ttcccggctt ttgtggataa tgtagttgtc cacgatcagc gtgatggttt tcgcacgacg
     8161 gtatgtcgct ttaagccgct tcagcaggct gatgaacagc gccgaacttt tgctgttgcc
     8221 gcccacatag ctgactttac ctgtcccgct gtgcagcgct ccggccagat aatatttttc
     8281 attctgtccc ggcgtgacca cccgtttttg ctgtccgcgc agttgccagt ccgcaccgat
     8341 tttgggatga agatggatat ccacttcatc ttcataaaag accggatgct ctgcgctgca
     8401 ttcgtccagt gctttatgga ttgctgccat cttttcatct ttatgcgggt cacggatacg
     8461 cagagttggc gca
//
LOCUS       DAJTRW010000144         8435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRW010000144 DAJTRW010000000
VERSION     DAJTRW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..178
                     /gene="csgC"
                     /locus_tag="OO326_003677"
     CDS             <1..178
                     /gene="csgC"
                     /locus_tag="OO326_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5967904.1"
                     /translation="QSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQA
                     LHLSQQWTPSSEKS"
     gene            299..610
                     /locus_tag="OO326_003678"
     CDS             299..610
                     /locus_tag="OO326_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5967905.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            705..1238
                     /gene="ymdB"
                     /locus_tag="OO326_003679"
     CDS             705..1238
                     /gene="ymdB"
                     /locus_tag="OO326_003679"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5967906.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1180..2661
                     /gene="clsC"
                     /locus_tag="OO326_003680"
     CDS             1180..2661
                     /gene="clsC"
                     /locus_tag="OO326_003680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5967907.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2669..3826)
                     /gene="mdoC"
                     /locus_tag="OO326_003681"
     CDS             complement(2669..3826)
                     /gene="mdoC"
                     /locus_tag="OO326_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5967908.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4220..5755
                     /gene="mdoG"
                     /locus_tag="OO326_003682"
     CDS             4220..5755
                     /gene="mdoG"
                     /locus_tag="OO326_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5967909.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5748..8291
                     /gene="mdoH"
                     /locus_tag="OO326_003683"
     CDS             5748..8291
                     /gene="mdoH"
                     /locus_tag="OO326_003683"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5967910.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2135 a         2000 c         2197 g         2103 t
ORIGIN      
        1 gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac ccattgcttt
       61 gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg ttactgtttc
      121 tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa agtcttaatt
      181 tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga atcagccaac
      241 ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa tagaataaat
      301 gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt ttattgccat
      361 tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg tggaaccggc
      421 atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga atggttctat
      481 tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa acgcgcaaat
      541 agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa tgaacatcga
      601 atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat ttaaccaaaa
      661 agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa acgcgtattc
      721 atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat gcggctaatc
      781 cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg ggtccggccc
      841 tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg gggcatgccg
      901 ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc gggccagtct
      961 ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg aatagcctac
     1021 gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt actggggttt
     1081 atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca gaatttatta
     1141 cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa aacgcccacc
     1201 tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct ggcgagcgcg
     1261 gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct ggagaaaagt
     1321 ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac gctggatgtt
     1381 cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc cctgttagcc
     1441 gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac gcccggactt
     1501 gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct ttttaatcct
     1561 ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg tctcaatcgc
     1621 cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg aggacgaaat
     1681 attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt agatgtcatg
     1741 gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg gtattgcaaa
     1801 tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc ggatcgcatc
     1861 gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg caaaatggaa
     1921 tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg ggcgaagaca
     1981 cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca ttcactgtta
     2041 ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat tatctcttcc
     2101 tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag aaaaggggta
     2161 aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt ccatgccgga
     2221 tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga actcaagccg
     2281 acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc cggtgccagc
     2341 ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc tttcaatttc
     2401 gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag cgaaacgctg
     2461 gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg gcagctccgt
     2521 ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga ggttattctc
     2581 aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc gtcgatattg
     2641 cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg ttctcacgct
     2701 taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa tgaatttcat
     2761 acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca agccagttgg
     2821 aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg tgaaccagat
     2881 agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag ttaagcaaac
     2941 ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg gtgatcaccg
     3001 actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt aaataagcga
     3061 caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac aaggctttaa
     3121 gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa aatagcgttt
     3181 gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca ataaaaatcg
     3241 ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat agttttacca
     3301 tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg cgcttaaata
     3361 tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg tgtgatatta
     3421 attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa ctttccgctt
     3481 tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg atggctgtta
     3541 acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag ggatagcgta
     3601 aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg cggaacgagt
     3661 ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc acatgccatg
     3721 tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc agccaggcgc
     3781 ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat ggttaactaa
     3841 tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg ccatagcgac
     3901 accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat catcgctacc
     3961 actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg gcaaacccta
     4021 cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg ttgcaatcag
     4081 gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc ggaatggctg
     4141 gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt gcttactaat
     4201 tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag taatgttaac
     4261 cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc aagctcaatc
     4321 cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt tccgcgacat
     4381 gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact ggaacaatct
     4441 gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata ccccggtcaa
     4501 aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg attatttcac
     4561 tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg ccggttttaa
     4621 agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca tgctcggggc
     4681 cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc gcggcctagc
     4741 aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt tctggatcga
     4801 gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact cgccgcgtgc
     4861 gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg atgtgcagtc
     4921 gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa ccagtatgtt
     4981 cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt tgcacgactc
     5041 taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt tgaataaccc
     5101 gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg gtctgttgca
     5161 acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc tccgtccaag
     5221 cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg tggaaattcc
     5281 aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc agctgccgga
     5341 gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg aagacaaact
     5401 gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg atgtgaagca
     5461 gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt ttaccggcgc
     5521 agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca ttggtgataa
     5581 tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct ggcgtctggt
     5641 gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg cgctggtgaa
     5701 tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg aataagacaa
     5761 ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg ccgaagactg
     5821 atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg cgggaggatg
     5881 attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat tcacttgctg
     5941 atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg ccagaagcaa
     6001 aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt ttctgggatc
     6061 gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa gaagagcagg
     6121 agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg ttgatcctga
     6181 cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct tatcagggtt
     6241 gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc tttatgcagc
     6301 ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg ttctgttggg
     6361 tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt ggtcgcgata
     6421 aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag catcgcacgg
     6481 cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc ttgcgtgcaa
     6541 cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac attctgagtg
     6601 acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag cttatcgctg
     6661 aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg aagcgtaaaa
     6721 gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac atggtggtgc
     6781 tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc ttgatggaag
     6841 ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg gatacgctgt
     6901 atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca gccggtttgc
     6961 acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc cgcgtgaaac
     7021 catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt gccggttcaa
     7081 tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg ggggtctgga
     7141 ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt gatgagctaa
     7201 aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc ctggttaagg
     7261 gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat ctctccgctc
     7321 cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat gcgttgaccg
     7381 aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag tggcgtcctg
     7441 agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg aagttattga
     7501 gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg cgcgttacat
     7561 tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc atgctgttcc
     7621 atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat tcaccgcagc
     7681 gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca cagctgctgt
     7741 tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg ttctggctgg
     7801 caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc agccgtgcca
     7861 ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag tattcaccgc
     7921 cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc tcccttgacg
     7981 atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc gcaatggcga
     8041 cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac gttgaacagg
     8101 cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg ctaagcgatc
     8161 cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga tattcttcat
     8221 gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa ccggatgcgg
     8281 cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac atcgttcctg
     8341 caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc acatccggca
     8401 aaaacaacga gccaataaaa ataccggcgt tatgc
//
LOCUS       DAJTRW010000145         8357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRW010000145 DAJTRW010000000
VERSION     DAJTRW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="OO326_003684"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="OO326_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /locus_tag="OO326_003685"
     CDS             1130..1237
                     /locus_tag="OO326_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967911.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="OO326_003686"
     CDS             complement(1290..2048)
                     /locus_tag="OO326_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5967912.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="OO326_003687"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="OO326_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5967913.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="OO326_003688"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="OO326_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5967914.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="OO326_003689"
     CDS             3761..4621
                     /locus_tag="OO326_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5967915.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="OO326_003690"
     CDS             complement(4662..5198)
                     /locus_tag="OO326_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5967916.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="OO326_003691"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="OO326_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5967917.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="OO326_003692"
     CDS             6176..6766
                     /locus_tag="OO326_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5967918.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="OO326_003693"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="OO326_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2167 a         1999 c         2006 g         2185 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcgg
//
LOCUS       DAJTRW010000146         8331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRW010000146 DAJTRW010000000
VERSION     DAJTRW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..157
                     /locus_tag="OO326_003694"
     CDS             <1..157
                     /locus_tag="OO326_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967919.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO326_003695"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO326_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5967920.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO326_003696"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO326_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5967921.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO326_003697"
     CDS             873..1244
                     /locus_tag="OO326_003697"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5967922.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO326_003698"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO326_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO326_003699"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO326_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5967923.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO326_003700"
     CDS             6947..7507
                     /locus_tag="OO326_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5967924.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO326_003701"
     CDS             7638..7850
                     /locus_tag="OO326_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5967925.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8331
                     /locus_tag="OO326_003702"
     CDS             8094..>8331
                     /locus_tag="OO326_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5967926.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2670 g         1662 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcaaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca t
//
LOCUS       DAJTRW010000147         8280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRW010000147 DAJTRW010000000
VERSION     DAJTRW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            53..361
                     /locus_tag="OO326_003703"
                     /pseudo
     CDS             53..361
                     /locus_tag="OO326_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            333..2243
                     /locus_tag="OO326_003704"
     CDS             333..2243
                     /locus_tag="OO326_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5967927.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2244..2447
                     /locus_tag="OO326_003705"
     CDS             2244..2447
                     /locus_tag="OO326_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5967928.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2444..4037
                     /locus_tag="OO326_003706"
                     /pseudo
     CDS             2444..4037
                     /locus_tag="OO326_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4027..5532
                     /locus_tag="OO326_003707"
     CDS             4027..5532
                     /locus_tag="OO326_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5967929.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5569..5916
                     /locus_tag="OO326_003708"
     CDS             5569..5916
                     /locus_tag="OO326_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5967930.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5974..7002
                     /locus_tag="OO326_003709"
     CDS             5974..7002
                     /locus_tag="OO326_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5967931.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7054..7440
                     /locus_tag="OO326_003710"
     CDS             7054..7440
                     /locus_tag="OO326_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5967932.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7452..7829
                     /locus_tag="OO326_003711"
     CDS             7452..7829
                     /locus_tag="OO326_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5967933.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7816..>8280
                     /locus_tag="OO326_003712"
     CDS             7816..>8280
                     /locus_tag="OO326_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5967934.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1925 a         1945 c         2552 g         1858 t
ORIGIN      
        1 ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc tcaaactact
       61 cattgccaga tgggaactga ccgcagaaca ggctgtcgca cagcagttaa aaaatcaggt
      121 gtcaaaagga aatcttatcg ataccggatt ttgtattttt gcccttagta agctggcgat
      181 ggctttatcc agtacgcttg actccattcc tttgtccatg caacgacagt tcccggattt
      241 aacgccgcgt catattgatc atctgaaaat ccttattgct aagggagcga atcagtgcgc
      301 gcgtgcgggg gataaattac cggatttact cgatgaatat atcagaacaa caactgagta
      361 atatgatggt ggcagtcact atcgcactgc agccactggt cagggtattg ccggtgacgg
      421 ctgtagaatg ggccgatcaa aattattacc tgcctaaaga atcgtcatac ggtgaggggg
      481 aatggaaaac gctgcagttt caggtcgcca tcatgaactg catgggcaat gacgagattc
      541 gtacagttaa cctgattaaa tctgcccgtg ttggttatac aaaaatggtg cttggtgtga
      601 tcgggtattt tattgagcac aaatcccgca acactcttct ttttcagccg acggactcag
      661 ctgctgaaga ttttatgaag tctcacgtgg aagcaacaat tcgtgatgtt ccttgcctga
      721 aaaaactttc tccctggctg ggccggaaac accgggataa tacgctcaca ctgaagcgtt
      781 tttcctctgg tgtggggttc tggtgtttgg gtggtgctgc tgccaaaaac taccgtgaaa
      841 aatctgtcga tgtggtctgc tatgacgaac tctcctcgtt tgaaccggat gtggaaaaag
      901 aaggttcgcc aacactgctt ggcgataaac gtattgaagg ttcggtatgg ccaaaatcca
      961 tacgcggttc aacgccaaaa attaaaggct cctgtcagat tgaaaaagct gcgaatgaat
     1021 ctgcgcattt catgcggttt tatgtccctt gccctcattg cggggaggag cagtatctga
     1081 agtttggcga tgatgcgacg tcgtttggcc tgaaatggga gaagggtaaa ccggaaacgg
     1141 tgtattacct gtgtgaacat aatggctgcg tgatccgtca gtctgaactt gatcagaccg
     1201 gggggcgctg gatttgtgac aataccggga tgtggacgtg tgacggtctg acgtttttca
     1261 gtgcttcggg taatgaaatt ccgccgccgc gctccatcac tttccatata tggacggcgt
     1321 acagtccgtt caccaccagg gtacagatag tctgtgactg gctggatgca ctgaaagatc
     1381 ccaacggcgt gaaaactttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
     1441 gcgaaaaact cgatcaccag gtactgatgg ataaggttgt gccttacacg gcaacggtgc
     1501 ctgtccgggt ggtttatctg acggcgggca ttgactcgca gcgaaaccgt tttgagatgt
     1561 atgtctgggg gtgggctccg ggagaggaag cctttctggt ggataaaatc atcatcatgg
     1621 gacgtcctga tgaggaagag acgctgttac gtgtggatgc ggcgatcaat aaaaaatacc
     1681 gccatgcaga cggtaccgaa atgaccattt cccgtgtctg ctgggatatc ggggggatcg
     1741 atggcgaaat cgtgtatcag aggtcaaaaa aacacggtat tttccgggta ctgccggtaa
     1801 aaggtgcctc tgtctatggc aagccggtga tcaccatgcc gaaaacccgc aatcagcggg
     1861 gtgtgtatct gtgtgaggtg gggacggata ccgcaaaaga aattctctat gcccgaatga
     1921 aggccgatcc cacccctgtg aatgaggcca catcgtatgc catccgcttt cctgataatc
     1981 cggagatttt ttcgcagaca gaggcacaac aactggtggc ggaggaactg gtggaaaagt
     2041 gggaaaaagg aaagatgcgt ctgttgtggg ataacaaaaa gcggcgtaac gaagcgctgg
     2101 actgcctggt gtatgcctat gcggcattac gtgtgtccgt gcaacgctgg caacttgatc
     2161 tggctgtgca gttgcatccc gcaaggatgc aactgcacaa ccgacccttg aagaactggc
     2221 agcgaagctg tccggaggag tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg
     2281 cgcgccttga tttattaacc ggtaaacggg ttgtgtcggt acagaaagac ggtcgcagga
     2341 tcgaatatac ggcagcgtcc ctgaatgagc ttaaccgtgc gatcaacgat gcggagtcgg
     2401 ttctgggtac gacccgacgc cgccgaccgc tgggagtgag gttatgaaac gaacgcctgt
     2461 cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg gaggcggcgc
     2521 aggatttggc gggcaaatgg cagagtggtt accaccggcg cagagtgccg atgcggccct
     2581 gttgcctgca ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca ataacggtat
     2641 cgcggctaat gcggtggccc tgcataagga tcatatcgtc gggcacatgt ttcttatcag
     2701 ctaccgtctg aactggcgtt ggctggggat gcgggagacc acggcaaaaa gttttgtcgg
     2761 ggaggtggaa gcagcctggt cggaatacgc agaaggaatg tttggcgaga tcgacgtgga
     2821 agggaaacgc acgtttacgg aatttatccg tgaaggggtg ggcgtgcatg cctttaacgg
     2881 cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc gtacgcgttt
     2941 taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga accactttct
     3001 gcgggccggg gttgaggtcg atcggtatgg gcgggcgatt gcgtaccata tctgtgagga
     3061 tgattttccg ttctctggtg ccggacgctg ggagcggatc ccgcgtgagc ttcccaccgg
     3121 gcgtccggcc atgctgcata tttttgagcc ggtggaggac gggcagaccc gtggggctaa
     3181 tcagttttac agcgtaatgg agcggatgaa gatgctggac tccctgcagg ccacgcagct
     3241 tcagtcggcc attgtgaagg cgatgtatgc agccacaatc gaaagtgaac ttgataccga
     3301 aaaggcattt gaatatatcg cgggggcacc gcaaggtcag caggataatc cgcttattaa
     3361 tattctggag aagttcaccc gctggtatga cacgaataac gtgacgctgg gcggtgttaa
     3421 aattccgcac cttttttcct ggcgatgcgc tgaacctgca gacagcgcag gattcagaca
     3481 atggattttc ggcgcttgaa caggcgctgc tgcggtatat tgcagccggt cttggcgttt
     3541 cctacgaaca gttgtcccgt gattactcga aggtcagtta ctcaagtgcc cgcgcctccg
     3601 ccaatgagtc gtggcgctat tttatggggc ggcgaaaatt tattgcggcc cggctggcca
     3661 cgcagatgtt ttcctgctgg ctggaagagg cgcttcttcg ggggattatt cgtccgccac
     3721 gggcgcgttt tgatttttac caggcgcgtt cagcctggtc acgggcagag tggattagct
     3781 ccggaagaat ggccattgac gggctcaagg aggtccagga atcggtgatg cgcattgagg
     3841 ccggactgag cacgtatgag aaagaactgg cactgatggg cgaagattat caggacattt
     3901 tccgccagca ggtcagggaa tccgcagagc gcgaaaaagc cggactctca cgtccggtgt
     3961 ggatagccca ggcgtatcag cagcagatag cggagagtcg caggccggaa gaggagacaa
     4021 catcacgtga gacgtaatct ttcacatatt atcgctgcgg cattcaatga gccgctgctt
     4081 ctggagcccg cctatgcgcg ggttttcttt tgcgcgctcg ggcgtgagat gggcgcagca
     4141 agcctttcgg taccgcaaca gcaggtacag cttgacgctc ccgggatgct ggctgaaacg
     4201 gacgagtaca tggccggagg caaacgacct gcccgtgttt accgggtggt gaacggtatt
     4261 gctgtactgc cggtgaccgg cacgctggta caccggctgg gtggcatgcg gccattttcc
     4321 ggaatgactg gctatgacgg cattgtcgcc tgtcttcagc aggcaatggc ggacactcag
     4381 gtacggggcg tactgctgga cattgacagt tctggcgggc aggccaccgg cgcgtttgac
     4441 tgcgctgaca tgatttaccg tctccggcag ctgaagcctg tctgggcact gtgcaatgac
     4501 acggcctgtt cagccgccat gttactggca tcggcctgct cccgacggct ggttacccag
     4561 acatcccgta tcggctccat tggggtgatg atgggccatg tcagctatgc cggtcatctg
     4621 gcacaggccg gtgtggatat cacgctgatt tacgccggat cgcacaaggt ggatggcaat
     4681 cagtttgaat ccctgccagc aaaggttcgc caggacatgc agcagcgcat tgatgcggcg
     4741 cgccggatgt ttgctgaaaa agtggcgatg tataccggtt tatctgtgga tgcggttatg
     4801 ggaacagagg ccgccgtttt tgaaggtcag tccggtattg aggccgaact ggcggatgaa
     4861 ttaatcaatg cgtcggacgc catcagcgtg atggctgcga cgctgaacac acacgataca
     4921 ggaggcacta tgccgcaatt aactgcaacg gaggctgccg cgcaggagaa ccagcgagtg
     4981 atggggatcc tgacgtgtca ggaagcgaaa ggacgtgaac aactggccac gatgctggca
     5041 gggcaacagg gtatgagcgt tgagcaggcc cgggcgattc tggccgtggc ggcaccgcaa
     5101 caatctggtg tatctgcgca gagtgaagcc gatcgcatta tggcatgtga agaggcgaaa
     5161 ggacgtgaac aactggcggc aacgctggcg gcgatgccgg agatgacggt ggaaaaagcc
     5221 cgtccgattc tggctgcttc accgcaggcg aatgccggac cctcacttcg tgatcagatt
     5281 atggccctgg atgaggcaaa aggggcagag gcgcaggctg aaaaactggc ggcctgtccg
     5341 gggatgaccg tggagaatgc ccgggctgta ctggcggcag cctcaggcag ggcagaaccg
     5401 gtttctgcat ccacaaccgc catgtttgaa catttcatgg cgaaccattc accggcagcg
     5461 gtacagggtg gcgtgccaca gacgtcagca gacggtgatg cggacgtgaa aatgctcatg
     5521 gccatgccat gaagtcagtg ctgaccatca atatgaggtt tttacaatat ggtaacgaaa
     5581 accatcactg aacagcgtgc ggaagtacgt atttttgccg gtaatgatcc ggcccatacc
     5641 gccacaggct gcagcgggat ttcctcggca acaccggctc tgacgcccct gatgctggat
     5701 gaggataccg ggaaactggt ggtctgggac ggacagaagg caggccatgt ggttggcata
     5761 ctggtactgc cgcttgaagg tacagagacg gtgctgacgt attacaagtc ggggaccttt
     5821 gcgacggagg ccatccactg gcctgagaga gtggatacac acaaaaaggc gaatgccttt
     5881 gccggcagtg ccctgagtca cgcagcgctg ccgtaacatt ttatcaggtc actgcggtag
     5941 cctgactgat ttctgaatga aaggaactga tttatgggat tgtttacgac ccgccagtta
     6001 ctcggttata ccgaacaaaa agttaaattt cgtgcgctgt ttctggagct gtttttccgc
     6061 cgtacggtga atttccatac cgaagaggta atgctggaca aaattaccgg aaaaacgccg
     6121 gtggcggcct atgtctcccc ggttgttgaa ggaaaggtgt tgcgtcaccg tggcggtgaa
     6181 actcgtgtac tgcgcccggg ctacgttaag ccgaaacacg aatttaatta ccagcaggcg
     6241 gtggagcgtc ttcccggtga agatccggcc cagctgaacg gcccggctta ccgtcgcctg
     6301 cgtatcatca ccgataacct caaacaggaa gagcacgcca ttgtccaggt ggaagagatg
     6361 caggcggtga atgccgtgct gtatggcaaa tacaccatgg aaggagagca gtttgaaact
     6421 gttgaggtcg attttggccg gtcggtggga aataacatta cgcaggcagg agggacggag
     6481 tggagcgctc aggaccgcga tacctttgat ccgacgcatg atctggatgc gtactgtgat
     6541 tttgcttccg gcaccatcaa tattgccatt atggatggta aggtctggcg tctgctgaat
     6601 ggctttaagc tgttccgcga aaaactggat acccgtcgtg gctcaaattc acagctggaa
     6661 acggcagtga aagacctggg ggcggtggtg tccttcaaag ggtattatgg tgatctggca
     6721 atcgtggtgg caaaaacgtc ctatgtggca gaggacggta ccgaaaaacg ttatctgccg
     6781 gacggcacac tggtactggg aaatacggcc gcagagggta ttcgttgcta cggtgccatt
     6841 caggatgcgc aggcattgtc tgaaggcgtg gtggcgtctt cccgttatcc gaaacactgg
     6901 ctgaccgtgg gtgatccggc gcgtgaattc accatgacgc agtccgcgcc gctgatggtg
     6961 ctgccggatc cggatgagtt tgtggtggtg caggtgaaat aatccgtgag cgggagctaa
     7021 atgctcccgt ttttcattca atcaggagcg ggtatggcta cgaaagaaga aaacctgaaa
     7081 cgtctttgtg agctggctga gaggctgggg cgtgaaccgg atgtctccgg tagtgcggca
     7141 gatattgcgc agcgagtggc ggaactggag gaagagcttg gagatgcggg ggaggtcgca
     7201 gagccggata cgtactcacc atcgaaagat gatccgaccg cgcataaagg cgagtcagtt
     7261 cctgaacagc caggcattgt taccggagac gaaacggggc aggttacggt ggtggcactg
     7321 gcgacactgc atacaaaatg taatggttca gtgatatttg tttcaccggg aacgtcgttt
     7381 cgtgtttctg ctgaggtggc tgccagcatg gcggcaggtg gtctggcaaa aaggcagtaa
     7441 caggagatgt cgtggacggt ttcgataatc tgtttgatgc tgcgcttgcc ggtgtcaatg
     7501 aagtcattct ccgtgatatg gggatcagcg ccgtcattac gtccggagaa cttgagggga
     7561 cacacctgac aggggttttt gatgatcccg aaagtatttc ttttgttgcc ggggggatcc
     7621 ggcttgagga ttcctcaccg tgcctgtttg tgaaaacggc agatatatcg cagttacgcc
     7681 gtcaggatac gttaactatc ggggatgatt ctttctttgt ggatcgtatt actccggatg
     7741 acgggggatg ctgttatatc cggttaaggc ggggcagtcc tgccccacag aataatgcca
     7801 gaatgaggcg atatgatgaa gggacttgag aatgccatcc gcaatctgaa cagcctcgat
     7861 acccgcatgg tgccacagac cagcgcatgg gcggtaaacc gtgtggcggc aaagattgtt
     7921 tctgtcgcca cacggcaggt tgcgcagaat accgttgccg gggataacca ggtgaaaggt
     7981 attcccctga aaacggtcag ggagcgtgtc aggctgctta aagccagccc ctcaggaaaa
     8041 atgtatgccc gcatgcgtgt taaccggggt aacctgcccg ccattaagct gggtaccgca
     8101 caggtcagac tggcccgctc ccggcatggc agcaactcac gtcatcgtgg cagcgtactg
     8161 aaagtgggga aatacctttt ccgggatgcg tttattcagc aactggcgaa tggtcgctgg
     8221 catgtgatgc ggcgtattga cggcaaaaat cgttacccca ttgatgtggt gaaaatcccg
//
LOCUS       DAJTRW010000148         8264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRW010000148 DAJTRW010000000
VERSION     DAJTRW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..393)
                     /locus_tag="OO326_003713"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO326_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO326_003714"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO326_003714"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5967935.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO326_003715"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO326_003715"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5967936.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6484
                     /locus_tag="OO326_003716"
                     /pseudo
     CDS             5797..6484
                     /locus_tag="OO326_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6077..6193
                     /locus_tag="OO326_003716"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6486..6852
                     /locus_tag="OO326_003717"
                     /pseudo
     CDS             6486..6852
                     /locus_tag="OO326_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6780..7253
                     /locus_tag="OO326_003718"
                     /pseudo
     CDS             6780..7253
                     /locus_tag="OO326_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            8006..>8264
                     /locus_tag="OO326_003719"
     CDS             8006..>8264
                     /locus_tag="OO326_003719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967937.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
BASE COUNT         2422 a         1899 c         1657 g         2286 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcggagtt cgaccgcctc tggaaaaaat
     6121 aatgccactg ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagtgaact
     6181 gcatattgcc ccatcaacgt attaccactg tcagcaacag cgacatcatc atgataaacg
     6241 cagtgcccgt gcgcagcgcg atgactggct gaagaaagag atactgcgcg tatacgatga
     6301 gaatcatcag gtatacgctg tgcgtaaagt ctggcatcag ttgttacggg aaggtatcag
     6361 agtggccaga tgcactgtgg cacgtctcat ggcggttatg gggcttgccg gtgttctccg
     6421 gggtaaaaag gtccacacta ccgtcagccg gaaagccgtt gccgcaggcg accgcgtaaa
     6481 ccgtcatcaa ggcaatgtgc cgcgtactcc gggtggcccg cagcggctgg tatacgtggt
     6541 gtcagcggcg gacaaggata aacacacgtc cgcagttccg tcagcactgc gacagcgttg
     6601 tccgcaaggc ttttacccag tccagcgtta cggtgcccca cgcctgacgg atgaactgtg
     6661 tgctctggtt accactttaa cgtaaaaacc gtggcagcca gtctgcgccg tcaggaactg
     6721 agcgcaaaag cctcccagaa gttcagcccg atcagttacc gtgcacacgg cctgcctgta
     6781 tcagaaaatc tgctgacgca ggatttttac gccagtggcc cgaaccagaa gtgggcggga
     6841 gacatcacgt actattacag ctcaccgact gcggggaaac acggggcgcc gggttattga
     6901 aggtgacctg tccagctact tcgacacagt acatcaccgg ctgctgatga aagccgtctg
     6961 ccgcaggatc agtgatacac gttttatgag actgctgtgg aaaacgccgt gttgatgttg
     7021 gtctcttccg ggcggtcagt gaaagtatgc cacagggcgg tgtgatctcg ccgttgctgt
     7081 cgaacatcat actgaatgag ttcgaccagt atctgaataa acgctacctg agtggaaaag
     7141 ccagaaagga tcgctggtac tggaatcaca gtatccagcg aggccgaagt actgctgtca
     7201 aagaaaactg gcaatggaaa cctgccgttg cttactgttg ctatgccgat gactgtgtgc
     7261 cacgaaggcg cgtcctgggg acgtgagtcc ctgtgcagcc atgcagcaca agagatgagg
     7321 gaaggcccct tggtgtcggc gtgcaggcgc aggcaccaaa ccatccactg ctgctgcatt
     7381 taagagatgt ggtggtgagc gagatgggaa aaggtcggac gtgagccgat caggtatggt
     7441 tcgaagagac gaacgaaagt gaaccgctga tgatgtgtcg aaagcatata gatgatgtca
     7501 aaacaatggt ggagtcgttc ctgtgggata tgtttactgc ctgcgcggca tccggcataa
     7561 aagcggcgtg aagtcgattc aggctcttgt ggggaacgtg gacctgtcac cccgatgtga
     7621 agggagaagc gcaagtggaa gccccatgag cgccagagta ccaatgcggg gtacagggac
     7681 ggagcaccaa gcgattgcgc agtaaacatg cttgacaact taattggggc cccctcttcc
     7741 ttttatggtg taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata
     7801 tttaatatac tttacaaaaa gtttgtatta gtggaataag gacaatagtt tatccaaact
     7861 gccaagcccc tttattataa gtaagatctg gtactaaata agcgttctaa accgccccga
     7921 gtaggcggcc tgacggctgc ggcttgtcat ggcctgtgat taccgttata aaaacagaca
     7981 atatcattgt ctttcaggta gttatatggc ccgttcagct aaaccacgca aacgaaaacc
     8041 tgcacctcaa agaagcaaac tcctccgcta tgtcgtaaaa cttcacgaag atgatttttt
     8101 tgacgaagaa gaagctgagg ttctgcgctt tgataatttt gacgatgccg ttgagtgttg
     8161 cgcagacctg aatattccgt tctttgtgga tgccggaaac aaaaaactgg tcttctggtt
     8221 tgtacgtgtt gatgacgaag ggtatccgga aattgcccgc tgca
//
LOCUS       DAJTRW010000149         8097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRW010000149 DAJTRW010000000
VERSION     DAJTRW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            7..657
                     /locus_tag="OO326_003720"
     CDS             7..657
                     /locus_tag="OO326_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967938.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO326_003721"
     CDS             629..1063
                     /locus_tag="OO326_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5967939.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO326_003722"
     CDS             complement(1836..2885)
                     /locus_tag="OO326_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5967940.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO326_003723"
     CDS             3336..4526
                     /locus_tag="OO326_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5967941.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO326_003724"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO326_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5967942.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO326_003725"
     CDS             complement(6373..7302)
                     /locus_tag="OO326_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5967943.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO326_003726"
     CDS             complement(7299..7661)
                     /locus_tag="OO326_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5967944.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2501 a         1545 c         1638 g         2413 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc caggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agtttcccat cctcccatct ctttaagcgc aagaaga
//
LOCUS       DAJTRW010000150         7958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRW010000150 DAJTRW010000000
VERSION     DAJTRW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..1277
                     /gene="yjeM"
                     /locus_tag="OO326_003727"
                     /pseudo
     CDS             1..1277
                     /gene="yjeM"
                     /locus_tag="OO326_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1428..4739)
                     /gene="mscM"
                     /locus_tag="OO326_003728"
     CDS             complement(1428..4739)
                     /gene="mscM"
                     /locus_tag="OO326_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5967945.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4761..5729)
                     /gene="psd"
                     /locus_tag="OO326_003729"
     CDS             complement(4761..5729)
                     /gene="psd"
                     /locus_tag="OO326_003729"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5967946.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5826..6878)
                     /gene="rsgA"
                     /locus_tag="OO326_003730"
     CDS             complement(5826..6878)
                     /gene="rsgA"
                     /locus_tag="OO326_003730"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5967947.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6973..7518
                     /gene="orn"
                     /locus_tag="OO326_003731"
     CDS             6973..7518
                     /gene="orn"
                     /locus_tag="OO326_003731"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5967948.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7729..7804
                     /locus_tag="OO326_003732"
     tRNA            7729..7804
                     /locus_tag="OO326_003732"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7762..7764,aa:Gly,seq:gcc)
BASE COUNT         1813 a         2063 c         2154 g         1928 t
ORIGIN      
        1 tcctggatga ataatagtgt cggaccacgt tttgccttca ttggtacgtt tatgtggttt
       61 tcctcttata tcatctggat ggtgagtacc tccgcgaaag tttgggtacc gttctcaaca
      121 ttcctctatg gtagtgacat gacccagcac tggcgtattg ccggactgga gcctacgcag
      181 gtggttggtc tgctggcagt agcatggatg attctggtca cagtcgttgc ttcaaagggg
      241 attaataaaa ttgcccgcat tactgcggtg ggcggtattg cagtaatgtg tctgaattta
      301 gtattgctgt tagtaagcat tactattttg ttattaaatg gtgggcattt cgcgcaggat
      361 attaatttcc ttgcatcacc gaacccgggt tatcagtccg gtctggcaat gctatcgttt
      421 gtggtatttg ctatttttgc ctatggcgga attgaagcgg ttggtggtct ggtcgataaa
      481 acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat tgttatttca
      541 atcggttatt cgctggcaat atttttatgg ggcgtcagca caaactggca gcaggtatta
      601 agtaatggtt ccgttaacct tggcaatatt acctatgtgc tgatgaagag cctcggggtg
      661 atgctgggta acgcactgca tttgtcacct gaagcgtcat tgtcgctggg cgtatggttt
      721 gcgcgtatta ccggactttc gatgttcctc gcttataccg gtgcgtttgc tattcaccgc
      781 tgaaagccat catccagggg acgccgaaag cgttgtggcc ggaaccgatg acgcgcctga
      841 atgcgatggg gatgccgtct atcgccatgt ggatgcagtg cgggttggtt actgtcttca
      901 ttctgctggt ttcgtttggt ggcggtaccg catcggcgtt ctttaacaag ctgacgctga
      961 tggcgaacgt gtctatgacg cttccttacc tgttcctcgc gctggctttc ccgtttttta
     1021 aagcacgtca ggatctcgac agaccatttg tgattttcaa aacgcgtatg tcggcaatga
     1081 ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa cgtcttcacc attattcagc
     1141 ctgtggttga agccggagac tgggacagca cattgtggat gattggcggc cctgtcttct
     1201 tctcgctgtt agcgatggcg atttaccaga actattgcag tcgcatggcg aataaacctg
     1261 agttagctct cgactgatca tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt
     1321 gtcaggaagt aatgcgtatg gatgatactc tccagggccg gtgataactg aagatgatgt
     1381 taagctgcta aatgagccac gttgacatca tttggcagct tcatgcatta caaacttccc
     1441 gcctgacgac ctttgcccgc agacgtcagc gttctccccg tttgtttacc gttgaggctt
     1501 tccagacgca tctggaaggg cgggaatggc atatcgatac cgtgggcgtg gaagcctgcc
     1561 agaatcagct ggtggatctc atggcgtagc ggcatacggt gacccatctc agcggcgtaa
     1621 atacgcagct cgaaaatctg aatcccctgt tgcagatcca ccaggaagac ttccggtgcc
     1681 gggttgtcga tcaccagcga gcagcgacgc gctgcggtga gcaggatttc cgtcacttct
     1741 tcactgttgg catcggcagg ggccggaatc gtcaacacta cgcgcgtgac cgagtcagag
     1801 agcgaccagt tgataaactg ctcggtaata aacgccttgt tcggcacgat tatctcttta
     1861 cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt ttgtcacgct accagtaaga
     1921 tcgcggatcg ttaccgtatc gccaatgcga atcggttttt cgaacaggat gatcaggcca
     1981 gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggca
     2041 accagccact gcaatttcga ccactcaata ccaatcattg agaagccgac cagcccgcca
     2101 atcagcatca gcagatattt tgtgatggtg gtgatggcgt aacccgtacc cggcgttaaa
     2161 tccaggtgct gcaaaatcgc cagttccagc agcgcgggca ggttgcgcac cagctgcgtg
     2221 gtgatgataa ataccagaat ggcaatcagc accgcaccga gggtaattgg ctccagactt
     2281 tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa
     2341 gcggaatgga tttctgacca cagcacaatg accgaaagca gggcgatcaa catcaaaatt
     2401 gagcgcacca gccgcaagga ttgcgcactg atggcatcga gatcgacttc gctttcatcg
     2461 acttcaattg ctccttccgg gctactgtga tgatgcgctt cctcttcgcc acgcgcacgt
     2521 tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa acgccagcct gcgacgctgg
     2581 atcagcatcc agcggcggat aacgtgataa accaccagta gcaggaacca gatggcaacc
     2641 gaggtttcaa gcctcgctaa cagtgcctgc gccgttgcca gataacccac cgccgacgcc
     2701 agaatggcaa ccagtggtgc gccaatcatc atgttccaca gcatatggtt ggtaatgttg
     2761 tcgccgctgc cttctttgtt gagatacaac ggaataccgg cttttttcag gcttagggtg
     2821 accaccgcca gcgcaccgca aatgaggata aagcaaagcc gtcccagcga accggaaaat
     2881 tcgcggtcgt cgaggttatc gaacatcatc agcgccataa tcagcccgat gctcatcagg
     2941 tagtagcgca tcccacggga aacacgttcg cgcggccagc caaaatgagc gataaacaag
     3001 ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc acagcagcgg cacggtggcc
     3061 gttacaccat cgccaatcgc gaccgccagc ggataaggcc acgcctcgcg caagccgtaa
     3121 cccagcgtca tccacagcac cggtaacggt gacgcgacga gaatcgacca gaaaagagtg
     3181 cgcaacgtca gccagaagtg atcctgagtc actttgccga ctttcgccgc cgaacgttca
     3241 agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat caacgcgcca
     3301 aacagcggca aaattgtctc tttgctggtc agcatcatca cactggcttt gcccaactga
     3361 ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg cgatttccag cggccaggcg
     3421 atggtcatcg ggcgcacgtc agaggcccag aacagatagc ggtgcgttgc ttcgttcacc
     3481 tctttcagcg catcctccag ttgcctgttg gagactttca gcttggtcag ttccaatagt
     3541 agcgtgtcgc caccctgcaa caatgagttc agcaactcac gctgagtgcg cagttgtgct
     3601 tccagaatac ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg gatttgccgt
     3661 agcagcggct gtttattaag taaatcctca taacgtaacc gttgcacacg caactgcgcc
     3721 atttcggtat caagctgttg tggttttggc atttctggta gccgtgccac ctgcgcccgc
     3781 agcgcttcgc cgagcaggtt ggacgatccc agccattgcg actgttcacg cagcgtattc
     3841 aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac gctgttgcga ggcaacgagc
     3901 tccatccgct gcgcctgttg attcagcgct gccgatagtt cgcggttaat tttgaattgc
     3961 gcgacgatat ctttcggcaa atcggcactg ttttctgcca gtaattcggt actttccagc
     4021 gcccgctccg cctcaagctg acgttggcta ttcaactgat tacgtaaggc ctgcaaatac
     4081 gcatccagtt gctggctctc tttttccgcc agctctgagc gtaagcgcgc taattcctgg
     4141 cggttattgg cagacagctg cgccagctcc agttcatcaa cgagcgcctt aagacgtgca
     4201 gagtcagact gcaacgcgaa attttgtgcc tgattgagcg gagtattgcc ggtaagcgtt
     4261 cccaggcggc gctcgatctc atttaactga cggcgggcgt cggtttgctg ttgcggcagt
     4321 tgattcagcg aatcggcaat ctcgcgggcg cgctcctgct cttgctgggc ctgacggctt
     4381 ttatccagca actggctgct gacctggaga atttcctgat tcagcgcgtc ggtagacatt
     4441 cccggcgaca cgctgcgcgg ctcgtcacga atgttgttta attgtgcgcg cagagtagcg
     4501 gagagtttcg gataattatc gataacttgc tgatattgtt tgatgcgctc aagggaacct
     4561 tttcgttcct caagcgcatt taaggcagac tggagcgcct ctacgacttc cggctgtgcg
     4621 ggtttcgccg cttttgcctg ctccagttcc tgagtgattt gtttgctatc gggggccgtc
     4681 gcggcgtacg ccccccaact gaggcaccag gccatcagaa aagtgataat caggcgcacg
     4741 tcagcgtttc ctttgatgga ttagacctgg tcttttttat cgtcaaccaa tgggctggcg
     4801 tcgtgttctg cttcgatctc ttcagcagga agtggggcgg gttcagcgtc tggcgtaaca
     4861 aaggtttcgg tagatactgc cagcggctgg ccaattttcg tgacagacag gctttccagt
     4921 tgctcaacca gattcacttt acccggtgca aacaggttga taacggtgga accgagttta
     4981 aagcgaccca tttcctggcc tttcagcagt gccacagaac cgtcgttttc cccggcaggc
     5041 caggtccagc gcttgatgat accttcgcgc ggcggcgtaa tggtgcccgc ccagaccgtc
     5101 tcaatgctgc caacaatcgt cgctccgacc agaatctgcg ccattgggcc aaattcggta
     5161 tcgaaaaggc aaatcacgcg ttcgttacgg gcaaacagat ttggcacgtt ctgagcggtg
     5221 agatggttaa cggaaaagag atcgcccggc acgtagatca tctcacgcag aataccgttg
     5281 cacggcatgt gtacgcgatg gtagtcacgc ggggagaggt aagtggtcac aaacgtaccg
     5341 ttgcggaaca ggtccgccat cagatagttg cctgccagca gggcttcgag gctgtagttg
     5401 tggcctttgg cttgcaggat tttatcttct tcgattttac ccaactggct gataacgcca
     5461 tcggcaggca tgaccagtac attcggatcg gtatcgattg ggcgtacttc gtcacgcagc
     5521 ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg tgtccggctt ttgcgcctct
     5581 ttcatgtcga ccttgtagta tttaacgaac agatcgataa ccagttttgt cagccatcct
     5641 gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc atagtttcgg cagaatgtac
     5701 tgtagcgaaa gtttaaatga atttaacaag gtagcctcca ggccattgtt ttgtcgttcc
     5761 tgatccggcc aacatgccgg atcctgaaaa aaaggggacg attctaacga cggttagctt
     5821 aattgtcagt catccgtatc agaaaagttt ttacgcgttt ttacctgcgc catgctttcc
     5881 agaatacggt gatagttttc gaaacgggtt tccgcgattt tcccttcctc aaccgcttcc
     5941 cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca cagacctaaa
     6001 tagtcatgga attcgacaaa gccctgagtg atttgttccg gctccaggtg ccagaggccg
     6061 aactcacgca caccagggga atcaatcaca tcaccgccgt gcgggaagtg atacagccga
     6121 gcggcggtcg tggtgtgctg accgagcccc gagttgtcag agacatcgtt ggtcaggatc
     6181 tctttttgca gccccagcaa cgcgttcagc aggctggatt taccgacgcc agactgcccg
     6241 gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg gtttcagccc atcctgagta
     6301 tggctggaaa ccatcaatac gcgataaccg atgttgcggt agatatccat ctgctcgttg
     6361 acgaacgcca tgccttcgtc gtccagcagg tctatcttgt tgagcacgat aatcggctca
     6421 atctgcaagg tttcgcaggc caccaggtaa cggtcgataa tattgagcga cagctccggc
     6481 aaaatggcgg agacaatgac aatctggtcg atgttggcgg ctatgggttt cacgccgtcg
     6541 tagaaatccg gacgcgttaa caccgaggtg cgctcatgca ccgcttccac gatccctttg
     6601 acatttacgc cttccgccgc cggtttaccc ggacgccaga ctacgcggtc gccggttacc
     6661 agcgaacgga tggtacggcg aatattgcag cggtgaacgt cgccatcggc ggattccaca
     6721 tcagcgtgca taccaaagcg gctgatgacg ataccttcat caggctcgcc aaacagattg
     6781 tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac gctggtgatt ggcgttcacg
     6841 cggcgctgct ggcctttgga gagtttattt ttactcaatc gtactgactc ctggtcgccc
     6901 ctgatgggca aaacatctat gatacacgca attgtggatc aatatagtca ctgtgaatgg
     6961 gtggaaaata gcatgagtgc caatgaaaac aacctgattt ggatcgatct tgagatgacc
     7021 ggtctggatc ccgagcgcga tcgcattatt gagattgcca cgctggtgac cgatgccaat
     7081 ctgaatattc tggcagaagg gccgaccatt gcagtacatc agtccgatga acagctggcg
     7141 ctgatggatg actggaacgt gcgcacccat accgccagcg ggctggtaga gcgcgtgaaa
     7201 gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc tcgaattctt aaaacagtgg
     7261 gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg gacaggaccg tcgtttcctg
     7321 tttaaataca tgccggagct ggaagtctac ttccactacc gttatctcga tgtcagcacc
     7381 ctgaaagagc tggcgcgccg ctggaagccg gaaattctgg atggttttac caagcagggg
     7441 acgcatcagg cgatggatga tatccgtgaa tcggtggcgg agctggctta ctaccgcgag
     7501 cattttatca agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc acttgcggcg
     7561 gcgtgatttt gacgctaaat cgaacgtctt gctgataatt tcagcacttg agataaaaac
     7621 gcaaaaaaaa cttttttggg gggttgcaga gggaaagatt tctcgtataa tgcgcctccc
     7681 gtaacgacgc agaaatgcga aaattacgaa agcaaaatta agtagtacgc gggaatagct
     7741 cagttggtag agcacgacct tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc
     7801 tccaaaattt gaaaagtgct gcaaagcaca gaccacccaa tcgcagtatt tcgaattgcg
     7861 acaaggcggc aactgaacga atccttggga acttacaaaa gtatgtgact aaggtgagtg
     7921 aaggcagcca aggcagtagc ggttcgaaag acgaagat
//
LOCUS       DAJTRW010000151         7909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRW010000151 DAJTRW010000000
VERSION     DAJTRW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(123..953)
                     /locus_tag="OO326_003733"
     CDS             complement(123..953)
                     /locus_tag="OO326_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5967949.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO326_003734"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO326_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5967950.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO326_003735"
     CDS             2379..2729
                     /locus_tag="OO326_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5967951.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO326_003736"
     CDS             2855..3415
                     /locus_tag="OO326_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5967952.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO326_003737"
     CDS             3418..6384
                     /locus_tag="OO326_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5967953.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO326_003738"
     CDS             6451..6828
                     /locus_tag="OO326_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5967954.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO326_003739"
     CDS             complement(7029..7688)
                     /locus_tag="OO326_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5967955.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAJTRW010000152         7724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRW010000152 DAJTRW010000000
VERSION     DAJTRW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO326_003740"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO326_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5967956.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO326_003741"
     CDS             1102..2562
                     /locus_tag="OO326_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5967957.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO326_003742"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO326_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5967958.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO326_003743"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO326_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5967959.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO326_003744"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO326_003744"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5967960.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO326_003745"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO326_003745"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5967961.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO326_003746"
     CDS             complement(6727..7245)
                     /locus_tag="OO326_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5967962.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7724
                     /gene="ydeI"
                     /locus_tag="OO326_003747"
     CDS             7521..>7724
                     /gene="ydeI"
                     /locus_tag="OO326_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5967963.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRG"
BASE COUNT         1933 a         1809 c         1954 g         2028 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggt
//
LOCUS       DAJTRW010000153         7682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRW010000153 DAJTRW010000000
VERSION     DAJTRW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..304)
                     /gene="csgB"
                     /locus_tag="OO326_003748"
     CDS             complement(<1..304)
                     /gene="csgB"
                     /locus_tag="OO326_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5967964.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSAND"
     gene            1058..1708
                     /gene="csgD"
                     /locus_tag="OO326_003749"
     CDS             1058..1708
                     /gene="csgD"
                     /locus_tag="OO326_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5967965.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1713..2102
                     /gene="csgE"
                     /locus_tag="OO326_003750"
     CDS             1713..2102
                     /gene="csgE"
                     /locus_tag="OO326_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5967966.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2127..2543
                     /gene="csgF"
                     /locus_tag="OO326_003751"
     CDS             2127..2543
                     /gene="csgF"
                     /locus_tag="OO326_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5967967.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2570..3276
                     /locus_tag="OO326_003752"
                     /pseudo
     CDS             2570..3276
                     /locus_tag="OO326_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3409..3900)
                     /locus_tag="OO326_003753"
     CDS             complement(3409..3900)
                     /locus_tag="OO326_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5967968.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4002..4556)
                     /gene="ycdY"
                     /locus_tag="OO326_003754"
     CDS             complement(4002..4556)
                     /gene="ycdY"
                     /locus_tag="OO326_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5967969.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4580..5317)
                     /gene="ycdX"
                     /locus_tag="OO326_003755"
     CDS             complement(4580..5317)
                     /gene="ycdX"
                     /locus_tag="OO326_003755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5967970.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5372..6310)
                     /gene="ghrA"
                     /locus_tag="OO326_003756"
                     /pseudo
     CDS             complement(5372..6310)
                     /gene="ghrA"
                     /locus_tag="OO326_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6545..6632
                     /locus_tag="OO326_003757"
     tRNA            6545..6632
                     /locus_tag="OO326_003757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6579..6581,aa:Ser,seq:gga)
     gene            complement(6782..7063)
                     /locus_tag="OO326_003758"
                     /pseudo
     CDS             complement(6782..7063)
                     /locus_tag="OO326_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7345..>7682)
                     /locus_tag="OO326_003759"
     CDS             complement(7345..>7682)
                     /locus_tag="OO326_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5967971.1"
                     /translation="SLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKR
                     GDKNLRRLLVQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAI
                     TTQKKEYQA"
BASE COUNT         2086 a         1842 c         1675 g         2079 t
ORIGIN      
        1 catcgttggc actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa
       61 tctttgcccg attgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct
      121 gccgtaactg agcactatta ttagtcccag cctgaccaat tatggctgcc tgattaaatg
      181 aagacttact caattcattt accgcgaagt tatattctga attagctaaa tcataacctg
      241 ctgcggctgc aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt
      301 tcatgttgtc accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt
      361 gttaacgcaa cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat
      421 atttttaata actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa
      481 aacccgcgta aatacgctgt tatctacgca aaaaatattt tgttttctct taaatctccg
      541 ttttccgcta tcaaaaatca ccagacagtc attcttcttg cccgtcgctg attgctgcga
      601 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa attgtccacg
      661 gaggtgtgga gaaaaaacaa aaacgtttta catgacgaaa ggactacacc gaaatatttt
      721 ttatatgtat tattagtaag ttagcaccat ttgtatgatt ttttaaaatt gtgcaataaa
      781 aaccaaatgt acaacttttc tatcatttct aaacttaata aaaccttaat gttaacattt
      841 taatataatg agttacattt tgttacatgt ttaacacttg atttaagatt tgtaatggct
      901 agattgaaat cagatgtaat ccattagttt tatattttac ccatttaggg ctgatttatt
      961 actacacaca gcagtgcaac atctgtcagt acttctggtg cttctatttt agaggcagct
     1021 gtcaggtgtg cgatcaataa aaaagtgggg tttcatcatg tttaatgaag tccatagtat
     1081 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
     1141 gcaccttaaa caatcgctag caattacagg aaaattacat aatattcaac gttctctgga
     1201 cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat
     1261 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
     1321 tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt
     1381 ttatgccatg gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgtggcga
     1441 atgctacttt acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta
     1501 taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg
     1561 tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa
     1621 aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc
     1681 ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg
     1741 cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat
     1801 tgttaactga ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata
     1861 aatgggaaag tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg
     1921 gaagctggat cacaataacg gtcaatcagg atgttatttt ccagactttt ttattcccgt
     1981 tgaaaagaga ctccgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa
     2041 atcgtcgcca gataaatcag gcgttattaa gtacggacga tttggcgcat gatgaattct
     2101 aaataaaaaa ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc
     2161 ttatttcgcc attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg
     2221 gtggtaaccc aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata
     2281 aagatccgag ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta
     2341 ctcaggccat ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac
     2401 cgggccgcat ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc
     2461 agttgaacgt gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac
     2521 aaaataactc aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt
     2581 atttcttttg gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc
     2641 cgccagaccg acattaatgc ctcgtgctca gagctacaaa gatttgaccc atctgccagc
     2701 gccgacgggt aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa
     2761 accctacccg gcaagtaact tctccactgc tgttccgcaa agcgccaggg cttacaaaac
     2821 ctgcttaacg agcgcaaggt tattcgtgcg gcacaagaaa acggcacggt tgccattaat
     2881 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatta tggttgaagg ttcgattatc
     2941 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     3001 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     3061 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     3121 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     3181 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     3241 aatgatggta tcgaccgtgg tctgtgggat ttgtaaaata aagcagaacg gcagaatgac
     3301 attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaatgccg
     3361 gaattaataa tccggcattt actgtttaag cgaaagcgtg agtgcccctc acgctttatt
     3421 tttgatttcc tgctcacggt gagccgtctt tgcactacgc gccgccagcc acaggccgct
     3481 gtttttcatt gcgtaaccaa ataccagccc aagcgccagc gaaggtaaca ccagtttcca
     3541 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag
     3601 cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc
     3661 gagaatttcc agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac
     3721 gccactaagc agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaagc
     3781 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
     3841 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
     3901 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
     3961 aacaaattat ctgttgcagt tatgagtgac gatgatcaca tttactcttc agaatcttct
     4021 tccagctcgt cccacattgc actaatggca tcacgggtta gcggtgccat ggtgcgccag
     4081 aaaggcgtgg ttgcatgggc ctccactttg ccgaggaacg caccacacca gggtaacaga
     4141 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
     4201 caggaagccg caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc cagtagcatc
     4261 cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta
     4321 cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
     4381 tgcgtcatat cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag
     4441 ttcgcagcca gtttcccctc acgaatcaga gtaaacagcg gcaccagtaa aggatcttgc
     4501 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat
     4561 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
     4621 attcaaggaa gttcagtaag cggcgcggag aaacattcaa aatgcgctct agcggaaaat
     4681 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag
     4741 aatccgagcc taatgccacc caacctccag catcacgtac cgctgcagct accgcacgac
     4801 agttgtcttc actgccctta cgtgagtgta aaaatgagga attattgatt tccagcgcca
     4861 cctgatgttt cgcggctgct tcagcaacgg ctttaacatc tatttcatat ttcggatttc
     4921 cgggatggct tattatatgc acattgccgc tggcgatagt ggagatcatc gcttgtgtat
     4981 tggtcgcttt gtcatgtggc gcaaaaaccg gctcatgaaa accggcaata atcagatcca
     5041 gcgagtcaaa cattttaccg ctgcagtcaa tttcaccatc aacattttta atgttagctt
     5101 caatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga
     5161 agtgccagtg atgcggcgca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt
     5221 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc
     5281 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
     5341 cgccaaggat agcaggaatc ctgatgcttt attagtagcc acgtgcgcgg tcgacttgcc
     5401 cgcagaccct ctcccctttt tcgagctggg caatagtgcg agaaatgtac tccacagctt
     5461 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
     5521 gcggactttc aggcggtaag ggttcacgat taaaaacatc cagcattgcg cctttaactt
     5581 tcccgctatc cagcgccgcg agcaggtcat cttccacaac atgaacacca cgcgccaggt
     5641 tgaggagata cgcgccatca ggtaattttt cgagtaattg ttgattaata atgccgacgg
     5701 tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca
     5761 gttcttcccg tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
     5821 aacgcagcgg aaagcgctag gtttgcagac tctgagcaac tttacagccc agtacgcctg
     5881 cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
     5941 aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
     6001 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
     6061 aagggttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca acaccggccc
     6121 cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccaga
     6181 ctaaaacata atcagcagag tcattatctc cgcttttcca tgccctgact cttgcctgag
     6241 gaatagcttt gcgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga
     6301 tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg
     6361 atctacaaag gccaccagca taacaaatcc gtggtcggtg gcaaaaaaag cagatttcgc
     6421 ttattaaaac cacacattga ttgaatgttt gaataaacgc gcgatttttt caaaaagttt
     6481 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     6541 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     6601 tatcgggggt tcgaatcccc ccctcaccgc cagattataa gtgcatgtta ttgtttaaca
     6661 tgcgcttttt tatttttcct tccagacagt atgccatcgg gtatgccatc tgaaaccggt
     6721 ttatatgccc cgcgatcata tcttatcgga caatttccga tctttgagca tactggcaaa
     6781 gctatactgc cagcatatca ggatagtgtt ttgcgataat atcattcagt ctgtcaacca
     6841 gttctggttt tctgaacgtg atatgcgcgg agcccttctt aaagtatctg atgctgaaca
     6901 tctcatcttc atagctctct ttaccctgaa cagactgaat gtgatcatcc agacgaaggg
     6961 tgatgtttcc ccggttgtcg gggattggtt taccgctgaa caggtgcaac attctttcca
     7021 gatcggcgag tcggtcagcc tgttgtccgg tgataagatg aaatctatgg tcacccccgt
     7081 ttttgcaaca ctgatttttg attaatggtt ggcttgctta aatctatccg gcgtctctgt
     7141 gggcaaggat gcccgcgccc cgatgagttc cgcacctgat aatcctaaaa atcccctcgg
     7201 cttcagtgag ccatttcatc tgttaggttt ttaatcaggt cgatttgccg ttcatgtcat
     7261 caataatcag tcttcgcaaa accagaaagg taacttgtat cttattgttt aaaataaact
     7321 aattgagtta tatcagcatc tatatcaggc ctgatattct tttttctgag tagtgattgc
     7381 ccaggcaatt cgagccagtt tatttgccag agcgcaggta accacattcg aatgcttctt
     7441 gttgagttgc tcccgaaccc actcagcaag ttttccatgc ttgcgttcaa gtcgcattat
     7501 gaatgagcgg gcacactgaa ccagtaaccg gcgtaaattt ttatcacctc gcttactgat
     7561 acccaacaga gtcgattttc ctccagtgct atactgccgg ggaacaagtc ctgtggaagc
     7621 tgcaaaatcc ctgctgcatg agaactgttt cccgtcacca agttgcgacg atagtaagct
     7681 gg
//
LOCUS       DAJTRW010000154         7374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRW010000154 DAJTRW010000000
VERSION     DAJTRW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..994
                     /locus_tag="OO326_003760"
                     /pseudo
     CDS             1..994
                     /locus_tag="OO326_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            999..1964
                     /gene="rbsC"
                     /locus_tag="OO326_003761"
     CDS             999..1964
                     /gene="rbsC"
                     /locus_tag="OO326_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5967972.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1989..2879
                     /gene="rbsB"
                     /locus_tag="OO326_003762"
                     /pseudo
     CDS             1989..2879
                     /gene="rbsB"
                     /locus_tag="OO326_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3005..3934
                     /gene="rbsK"
                     /locus_tag="OO326_003763"
     CDS             3005..3934
                     /gene="rbsK"
                     /locus_tag="OO326_003763"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5967973.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDKIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3938..4930
                     /gene="rbsR"
                     /locus_tag="OO326_003764"
     CDS             3938..4930
                     /gene="rbsR"
                     /locus_tag="OO326_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5967974.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4896..6323)
                     /gene="mdtD"
                     /locus_tag="OO326_003765"
     CDS             complement(4896..6323)
                     /gene="mdtD"
                     /locus_tag="OO326_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5967975.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6346..7038)
                     /locus_tag="OO326_003766"
     CDS             complement(6346..7038)
                     /locus_tag="OO326_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5967976.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1868 a         1795 c         1945 g         1766 t
ORIGIN      
        1 cgctgaccga taccgaaacc gaatccctgt tccgcgtcat ccgcgagctg aaatcgcaag
       61 gccactgtat tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg
      121 ttaccgtttt tcgtgatggg caatttattg ctgagcgcga agtggcatca ctgaccgaag
      181 attcgctgat tgagatgatg gtgggccgca agctggaaga tcaatatcca cacctggaca
      241 aagcgccagg agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg
      301 tctcttttac tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc
      361 gtaccgaact gatgaaagtg ctctacggcg cactgccgcg caccagcggt tacgtcaccc
      421 tggatgggca tgaagtcgtt acccgttcac cgcaggatgg cctggcaaac ggcattgtgt
      481 atatctccga agaccgtaaa cgtgacggtt tagtgctggg catgtcagta aaagagaaca
      541 tgtcgctgac agcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg
      601 aacagcaggc tgtgagtgat ttcattcgtc tgtttaatgt gaaaacgccg tcgatggaac
      661 aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga
      721 tgacaccccc aaagtattga tccttgatga gcctacccgt ggcgtagatg tcggcgcgaa
      781 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagta tcattctggt
      841 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
      901 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgtcgc
      961 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gataacccag actgtctctg
     1021 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
     1081 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
     1141 atattctcca gcaaacttcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
     1201 tgacgtcggg catagacttg tcggtaggtt ctctgttggc gctaaccggc gcagttgctg
     1261 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgctgct ctcacgttag
     1321 gtgccgcaat tggtgcggta accggggtga ttgtagcgaa aggtcgcgtc caggcgttta
     1381 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaacggta
     1441 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
     1501 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
     1561 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
     1621 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
     1681 gtctgctggc atcgctggcc gggatcattg aagtggcgcg tctctcctct gcacaaccca
     1741 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
     1801 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
     1861 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
     1921 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
     1981 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
     2041 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
     2101 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataaa cttggctata
     2161 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
     2221 taaccgttcg cggcaccaaa attctgctga ttaacccgac cgactccgac gcagtgggta
     2281 atgctgtgaa gatggctaac taggcgaaca ttccggttat cactcttgac cgccaggcaa
     2341 cgaaaggtga agtggtgagc cacattgcat ctgataacgt actgggcggc aaaatcgccg
     2401 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
     2461 ccggtacatc cgcagcccgt gaacgtggcg aaggcttcca gctggccgtt gctgctcaca
     2521 agtttaatgt tcttgccagc cagccagcag attttgatcg caccaaaggt ttgaacgtaa
     2581 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
     2641 tggcgctggg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
     2701 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
     2761 tcgctcagct acccgatcag attggcgcga aaggcgttga aaccgcagat aaagtgctga
     2821 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
     2881 tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
     2941 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
     3001 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
     3061 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
     3121 atttggcggc aaaggcgcga atcaggctgt ggctgctggg cgtagcggtg cgaatatcgc
     3181 gtttattgcc tgtactggcg atgacagcat tggcgagagc gttcgccagc agctcgccac
     3241 tgataaaatc gatattactc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
     3301 gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc
     3361 tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cagcgttatt
     3421 aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
     3481 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aactgctggc
     3541 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
     3601 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaagg gtatccgtac
     3661 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
     3721 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
     3781 cgcgttaatc acggcgttgc tggaagaaaa accattgcca gaggcgattc ggtttgccca
     3841 tgctgccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
     3901 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
     3961 ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
     4021 cagtgaagcg attaccgcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
     4081 agctctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
     4141 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
     4201 cggttatagt ctcgtccttt gcaataccga aggcgatgaa cagcggatga atcgcaatct
     4261 ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta ctgtgtaccg aaacgcatca
     4321 gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct accgtgatga tggactgggc
     4381 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
     4441 aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc tgtattaccg gtccgctgga
     4501 taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct
     4561 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
     4621 catgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
     4681 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
     4741 cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaaccac
     4801 tatccaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
     4861 aacccagccg acccttcagc aacaacgatt acaacttact ccgattctga tggaacgcgg
     4921 ttcggcatag atttacgctg tcttttgatc aaattattac catcggttgt tttcagaagc
     4981 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
     5041 tgttcgacag tcgttgtgcc ttccatccct tcataaatgc gaaggacggc cgcacttaca
     5101 gcaacgccta aactaatcga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
     5161 gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ctgcgtcgac
     5221 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgccggtgat
     5281 tgcaaagaga actgagcgat cattagccca ataatcaccg tgatccccac taatgtatgg
     5341 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
     5401 gctgtcggtg ccatcataca gccggcaata aacgcctgat aaccaaatcc tacctgtaac
     5461 atcaatggca taaggaacgg tacacagccg gtccccagac gggttgcaat attgcctacg
     5521 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgta
     5581 cgtcgcgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat taccgtcaag
     5641 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga aaagagaaca
     5701 aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa
     5761 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attaattaaa
     5821 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
     5881 ggccccacca gacccggcat ggcgacaaaa ttcaataccg gaagaagttc attacgagga
     5941 taagcacgca gtaaggccgg ccgagcaaca ggcatcatca ttgcgtcgcc tatcccttga
     6001 ataacccgga agacaaccag ctgtggtagc gaattagaaa gtgcgcaggc cagagaaccc
     6061 aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg taccgaagcg atcggctagc
     6121 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
     6181 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
     6241 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgacata
     6301 ctacgcttct ttttatcgct cattcaatgt cctgttatcg ggttatcact tatcaggtga
     6361 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc
     6421 gacaatcgcc tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
     6481 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
     6541 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
     6601 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt
     6661 gccaaccgtt gctgccagta ggcaggcttg cggctccaga cagatacgca taaccagaaa
     6721 gtgatcgatg acctgatgaa agttctcttc tgttatccac caagtaagca attcctgatc
     6781 aagaaaattc cagttcgatt gtggcatgac ccgagtacca attcgcggtc gcggtaaaac
     6841 catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa
     6901 ttgctcgccc agctcaatct caccgggcaa aatggtgccg ggttcatatt caccttttaa
     6961 gatccgttgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
     7021 ttgtgcgctt aaaggcatta cttatcttcc tttttctttt tattcctcct tagtatgcca
     7081 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg
     7141 gggtgttttg ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc
     7201 cgggataact ccctataata cgccaccact gacacggaac aacggcaaat acgccgccgg
     7261 gtcagcgggg ttctcctgag aattccgaca gaaaaagcaa aaataaatga ttgactctgt
     7321 agcgggaaag cgtattatgc acaccccccc ccgcgccgct gagaaaaagc gaag
//
LOCUS       DAJTRW010000155         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRW010000155 DAJTRW010000000
VERSION     DAJTRW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO326_003767"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO326_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OO326_003768"
                     /pseudo
     CDS             764..916
                     /locus_tag="OO326_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OO326_003769"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OO326_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5967977.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OO326_003770"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OO326_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OO326_003771"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OO326_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OO326_003772"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OO326_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5967978.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OO326_003773"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OO326_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5967979.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7086..>7188)
                     /locus_tag="OO326_003774"
     CDS             complement(7086..>7188)
                     /locus_tag="OO326_003774"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5967980.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2252 a         1356 c         1385 g         2195 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttgggaagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaatcgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct gtactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaagttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcaca cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc acttttcata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcacacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggtg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagaga attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactattt
//
LOCUS       DAJTRW010000156         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRW010000156 DAJTRW010000000
VERSION     DAJTRW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(296..1198)
                     /locus_tag="OO326_003775"
     CDS             complement(296..1198)
                     /locus_tag="OO326_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5967981.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO326_003776"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO326_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5967982.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO326_003777"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO326_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5967983.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO326_003778"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO326_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5967984.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO326_003779"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO326_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5967985.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO326_003780"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO326_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO326_003781"
     CDS             7090..>7183
                     /locus_tag="OO326_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5967986.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTRW010000157         7178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRW010000157 DAJTRW010000000
VERSION     DAJTRW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            18..869
                     /gene="hchA"
                     /locus_tag="OO326_003782"
     CDS             18..869
                     /gene="hchA"
                     /locus_tag="OO326_003782"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5967987.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO326_003783"
     CDS             complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO326_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5967988.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO326_003784"
     CDS             complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO326_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5967989.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3139..3552
                     /gene="hiuH"
                     /locus_tag="OO326_003785"
     CDS             3139..3552
                     /gene="hiuH"
                     /locus_tag="OO326_003785"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5967990.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3661..4665
                     /gene="msrP"
                     /locus_tag="OO326_003786"
                     /pseudo
     CDS             3661..4665
                     /gene="msrP"
                     /locus_tag="OO326_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4666..5301
                     /gene="msrQ"
                     /locus_tag="OO326_003787"
     CDS             4666..5301
                     /gene="msrQ"
                     /locus_tag="OO326_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5967991.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5559..6209
                     /gene="zinT"
                     /locus_tag="OO326_003788"
     CDS             5559..6209
                     /gene="zinT"
                     /locus_tag="OO326_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5967992.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2027 a         1627 c         1506 g         2018 t
ORIGIN      
        1 acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc gatattgctg
       61 aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt cccgtctctg
      121 atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt ctggtgatcg
      181 ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc ggtaaccatc
      241 cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa ttcgaagtgg
      301 cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac aaagatgaaa
      361 aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag aaactcgcgg
      421 atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt cctggtgggc
      481 atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta cagtgggcaa
      541 tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt ctggcacttc
      601 gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac gccgcagaca
      661 aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc ggcgaagagt
      721 tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta cataaggacc
      781 gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa ctggcggcgc
      841 aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta agcgcataat
      901 caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt aacttatggc
      961 gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg ttatgcttac
     1021 tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg actaaagaaa
     1081 ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat ctacggaaga
     1141 gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta agagagccgt
     1201 tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat cgaatggcgt
     1261 taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag atttgttgat
     1321 tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga tagtccaaaa
     1381 gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
     1441 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
     1501 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
     1561 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
     1621 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
     1681 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
     1741 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
     1801 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
     1861 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
     1921 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
     1981 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
     2041 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
     2101 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
     2161 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
     2221 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     2281 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     2341 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     2401 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     2461 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     2521 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     2581 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     2641 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     2701 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     2761 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     2821 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     2881 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     2941 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     3001 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     3061 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     3121 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagcc
     3181 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     3241 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     3301 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     3361 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     3421 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     3481 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     3541 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     3601 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     3661 atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
     3721 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     3781 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     3841 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     3901 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     3961 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     4021 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     4081 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     4141 ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     4201 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     4261 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     4321 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     4381 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     4441 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     4501 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     4561 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     4621 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     4681 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     4741 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     4801 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     4861 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     4921 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     4981 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     5041 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     5101 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     5161 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct ggctgtactg
     5221 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     5281 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     5341 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     5401 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     5461 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     5521 attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     5581 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     5641 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     5701 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     5761 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     5821 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     5881 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     5941 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     6001 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     6061 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     6121 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     6181 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     6241 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     6301 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     6361 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     6421 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     6481 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     6541 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     6601 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     6661 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     6721 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     6781 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     6841 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     6901 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     6961 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     7021 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     7081 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     7141 cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAJTRW010000158         7158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRW010000158 DAJTRW010000000
VERSION     DAJTRW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            17..1075
                     /locus_tag="OO326_003789"
     CDS             17..1075
                     /locus_tag="OO326_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5967993.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="OO326_003790"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="OO326_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5967994.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /locus_tag="OO326_003791"
     CDS             complement(1622..1726)
                     /locus_tag="OO326_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5967995.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="OO326_003792"
     CDS             1896..2342
                     /locus_tag="OO326_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5967996.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO326_003793"
                     /pseudo
     CDS             complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO326_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="OO326_003794"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="OO326_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..4796
                     /locus_tag="OO326_003795"
     CDS             4449..4796
                     /locus_tag="OO326_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5967997.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO326_003796"
     CDS             complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO326_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5967998.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5255..6280
                     /gene="dgcP"
                     /locus_tag="OO326_003797"
     CDS             5255..6280
                     /gene="dgcP"
                     /locus_tag="OO326_003797"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5967999.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6313..6411
                     /locus_tag="OO326_003798"
     CDS             6313..6411
                     /locus_tag="OO326_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5968000.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO326_003799"
     CDS             6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO326_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5968001.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6649..6840)
                     /locus_tag="OO326_003800"
                     /pseudo
     CDS             complement(6649..6840)
                     /locus_tag="OO326_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7020..>7158)
                     /locus_tag="OO326_003801"
     CDS             complement(7020..>7158)
                     /locus_tag="OO326_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968002.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
BASE COUNT         1727 a         1742 c         1744 g         1945 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatgcccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag
     4681 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     4741 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     4801 agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     4861 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     4921 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4981 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     5041 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct
     5101 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     5161 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     5221 tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt
     5281 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     5341 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     5401 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     5461 cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga
     5521 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     5581 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     5641 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     5701 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc
     5761 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     5821 tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc
     5881 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     5941 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     6001 tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     6061 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     6121 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     6181 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     6241 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     6301 attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc
     6361 gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa
     6421 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     6481 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     6541 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataacggc
     6601 tgaagcggcg cacgaaaaac gctttcgcgt ttttcgtgcg ccgcttcacc cctggttcag
     6661 catttcgtct gcagtctgaa ccgcaggcaa ccatttttca gcaagtctgc gctgggaatc
     6721 gctggcttta tcccatttgc tccacagtgc ctcacgatcg taatcgtgcg cttccagcgt
     6781 ggggcgggcg atttcaaaat acccctagct tgtttctatt tgctcagatt ttaataacaa
     6841 ctcagcacgt gctgccgctg gtagaactga tatatgaaat tccaaagcct ttgaacccgt
     6901 acgcttgcga cgtagttctg gatgattggc tgcaagcttt tcaagattgc ggcgaatact
     6961 cacctcatgt ttaggaaagc atgacattcc tacgcattct tgcgctgtta cccaatgggt
     7021 taacatgatg ttttctccat ataccgagaa ggccaaatct gggctggttc catatccaaa
     7081 tatctagcaa tgattgcttc gtatcgtggt atatggcagc gaaatacgtt acgcatagtg
     7141 tcaggcttta agcctgcc
//
LOCUS       DAJTRW010000159         6982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRW010000159 DAJTRW010000000
VERSION     DAJTRW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..417
                     /locus_tag="OO326_003802"
     CDS             <1..417
                     /locus_tag="OO326_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5968003.1"
                     /translation="LQSLVGGTVVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIE
                     QCSELSAVSASFVLATPTETDGAVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKP
                     TTDIRKDKCSKCMRGCELRNNTGNFGGFLSINKLSQ"
     gene            422..1165
                     /locus_tag="OO326_003803"
     CDS             422..1165
                     /locus_tag="OO326_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5968004.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            1102..1704
                     /locus_tag="OO326_003804"
     CDS             1102..1704
                     /locus_tag="OO326_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5968005.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1765..5244
                     /locus_tag="OO326_003805"
     CDS             1765..5244
                     /locus_tag="OO326_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5968006.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            5312..5911
                     /locus_tag="OO326_003806"
     CDS             5312..5911
                     /locus_tag="OO326_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5968007.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5975..>6982
                     /locus_tag="OO326_003807"
     CDS             5975..>6982
                     /locus_tag="OO326_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5968008.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT         1595 a         1713 c         2207 g         1467 t
ORIGIN      
        1 ctgcagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat
       61 gcggtgaact tcgtcaacgg aaacagcgac gccgatccgg aacaggaggt gatcagccgc
      121 tggcgcatcg agcagtgcag cgaactgagc gcggtcagtg cctcttttgt gctggccaca
      181 ccaacggaga cggatggcgc ggttttcccg gggcgtatca tgctggcgaa tacctgtatg
      241 tggacttacc gttctgatga gtgtggttac acgggcaggg cagtggctga cgagttcgac
      301 aaaccaacaa cggatatccg gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg
      361 cgcaacaata ccggtaattt cggcggtttc ctttccatca ataaactttc tcagtaaatc
      421 catgacacag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc
      481 gtgtggcttc gtggtgagaa cgccggaggg agacaggtat cttcccagcg agaatatctc
      541 cggtgagccg gaggaacggt tccggatggc tccggaggac tggctgcggg cacaaatgca
      601 gggtgagatt gtggcactgg tccacagtca tcccggtggt ctgccctggc tgagtgaggc
      661 tgaccggcgg ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc gggggacgat
      721 tcacaagttc cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg gggtgacgga
      781 ctgttacacg ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca
      841 tcgcggggat gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac
      901 agggctgtat caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt
      961 tggttcatcg gtgccgaatc atgccgccat ttactgcggc gatggtgagc tgctgcacca
     1021 tattcctgaa caactgagta aacgagagag gtataccgac aaatggcagc gacgcacaca
     1081 ctccctctgg cgtcaccggg aatggcacgc atctgccttt acggggattt gcaacgattt
     1141 ggccgccgca tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc actggccaca
     1201 cagctcccgg cgtttcgtca gaaactgagt gacggctggt atcaggtgcg cattgccggg
     1261 cgtgatgcag gtgaaaccga attgtctgcc cgtcttaatg agccgctggc aaatggtgca
     1321 gtgatccaca tagtaccgcg tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg
     1381 ctgggggcag ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt
     1441 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca
     1501 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc
     1561 tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg
     1621 cgcgtggggt cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt
     1681 caggttgtgg tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt
     1741 tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc
     1801 gaagcgaagg acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa
     1861 gggccggttg acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc
     1921 ctggatagcg aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag
     1981 caggagcaga caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg
     2041 gaagtgaaat acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg
     2101 cgcctgacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg
     2161 tcggaagtcc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
     2221 atcaccatta agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg
     2281 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag
     2341 ctgcagaaca aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac
     2401 ccgaacacgg cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg
     2461 agtcgcaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
     2521 acgcggcaat acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg
     2581 gcctggtgtc tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt
     2641 gcggcagatg tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg
     2701 ccggacggat ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag
     2761 cgtaaggcgt gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
     2821 aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac
     2881 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
     2941 aaggaccgcc ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg
     3001 gcgacagagc ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag
     3061 atggatgcct ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt
     3121 aaaacggaac tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc
     3181 catgtaccgg gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt
     3241 ggtcgcgtac tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
     3301 ctgccgtcct ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc
     3361 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc
     3421 gttgcagaat acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc
     3481 tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
     3541 ccggaaaaag aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg
     3601 gtgaatggtg tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc
     3661 ggggaatatc aggtgctggc gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc
     3721 ctgcttcgcc tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacagccagg
     3781 acgacggaaa ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc
     3841 cgggcggtaa atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg
     3901 gcaccggcag cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc
     3961 ccgaaactgg ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg
     4021 attgcggata tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg
     4081 atagccgcca gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg
     4141 aacaccgttg gcaaatcggc atttgtggag gccgtcggtc gggcgagcga tgatgcggaa
     4201 ggttatctga gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg
     4261 acgcagattg ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg
     4321 aatgtcagca atgaaatcac gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca
     4381 atccagcaga tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct
     4441 gtgaagctgc aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt
     4501 gagaatacgc cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg
     4561 attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc
     4621 atgaacgacg tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct
     4681 ccggcatttt ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt
     4741 aacgtgaacg cgaactccgg gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt
     4801 ctgggaaaat tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct
     4861 ttcccacggg actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt
     4921 tatgacgatc agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct
     4981 aaacatgagc ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac
     5041 ggtgctgaaa tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt
     5101 gatatgcctg ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta
     5161 aatggctggt atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc
     5221 acagcaggta tcagtatcag ctgaatttta taacccagat acgggcacca gaaatggtgc
     5281 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc
     5341 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcggtccac
     5401 gctgagcgcg gggtatcttc atgcctcgac gaacgctccc ggcagtgatg atctgaacgg
     5461 gattaacgtg aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag
     5521 ctatgccaac gctgaagatg agcaaaaaac gcattacagt gatacccgct ggcatgagga
     5581 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggctg tctgtgcgcg tgaatgaatg
     5641 gttcagcgcg tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga
     5701 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
     5761 caaccgccac agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga
     5821 atccgtggcc attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
     5881 tttcatcgtg ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc
     5941 ccgctggttc aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact
     6001 gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac
     6061 cagtccgacc gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta
     6121 tgtgattgag gctgagccgg ggtattacga ggttgcgctt cactgtaacg gctggcagcc
     6181 aacccgtgtc ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt
     6241 tctgaatgca ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat
     6301 ggtggcgcag gcgcagcaga gtgccggggc tgcagccgga aacgcacagc agacggcgca
     6361 ggatgtggcg gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg
     6421 acaggatgca accgtcacag ctgaggacag aaaggccact gcggaagatg tgacaagcac
     6481 aggagcaaat gcagccgcag ccggacagag cgcacaggat gccgcaggtt atgcccgcgc
     6541 agcagaacag gccaaaaatg acattgatgc tgcgctgacc ggcactctga aaatggctaa
     6601 ccatctgtca gaaatcgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct
     6661 ggggctgaaa agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg
     6721 ccgtctggcg atacccggcg cattcggctt tgggtgtgct tttctgcctg aagatgttat
     6781 ccgttttgac actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtgaata
     6841 ttccgttgct ggcccctacg gcatcatcat acccgacaca cggtttgaag gggtgctcag
     6901 catccggtgg actgatgcac gccctgagac aacagaaccg cggtacagag ccaaatccct
     6961 tactttttac ggcattaacg gc
//
LOCUS       DAJTRW010000160         6965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRW010000160 DAJTRW010000000
VERSION     DAJTRW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            256..1680
                     /gene="sbcB"
                     /locus_tag="OO326_003808"
     CDS             256..1680
                     /gene="sbcB"
                     /locus_tag="OO326_003808"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5968009.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1723..1950)
                     /locus_tag="OO326_003809"
     CDS             complement(1723..1950)
                     /locus_tag="OO326_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5968010.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1964..3022)
                     /gene="yeeE"
                     /locus_tag="OO326_003810"
     CDS             complement(1964..3022)
                     /gene="yeeE"
                     /locus_tag="OO326_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5968011.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3201..4559)
                     /gene="plaP"
                     /locus_tag="OO326_003811"
     CDS             complement(3201..4559)
                     /gene="plaP"
                     /locus_tag="OO326_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5968012.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4549..4611)
                     /gene="yoeI"
                     /locus_tag="OO326_003812"
     CDS             complement(4549..4611)
                     /gene="yoeI"
                     /locus_tag="OO326_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5968013.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4772..5755)
                     /locus_tag="OO326_003813"
     CDS             complement(4772..5755)
                     /locus_tag="OO326_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5968014.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5801..6625)
                     /locus_tag="OO326_003814"
     CDS             complement(5801..6625)
                     /locus_tag="OO326_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5968015.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6708..6962)
                     /gene="yoeB"
                     /locus_tag="OO326_003815"
     CDS             complement(6708..6962)
                     /gene="yoeB"
                     /locus_tag="OO326_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5968016.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1892 a         1817 c         1624 g         1632 t
ORIGIN      
        1 gcatcagcca cttcttgtgg gcccgaatat ggtttaccat aaatagtacg cagttttgtg
       61 tcgcttatag ccataaataa tatttggtac gctcagaaat aatagtttgg cacacatctg
      121 gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac
      181 atactcaggt gaaaaaaggg ttgcttttcg agttataaca gacagttaat taactacagt
      241 ggacaatgct agacaatgac agacacagat aaacaaccta ccttcctctt tcacgattac
      301 gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc
      361 gatgacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac
      421 tatttacccc ggcctggagc agtattaatt accggtatta ccccgcagga ggcacgggcg
      481 aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag
      541 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
      601 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
      661 ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
      721 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa
      781 catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg
      841 gtaaaaacgc gtcagccacg cctgtttgat tatctcttta cccatcgtaa taaacacaaa
      901 ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ctcacgtttc cggaatgttt
      961 ggggcatggc gtggcaatac cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc
     1021 aatgccgtaa ttatggcgga tttggcagga gacatttcgc cattactgga gctggatatc
     1081 gacacattgc gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt
     1141 ccggttaagc tggtgcatat caataaatgt ccggtactgg cccaggcgaa tacgctacgc
     1201 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt
     1261 ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtagcga tattcgcgga agccgaaccg
     1321 tttacacctt cagataatgt ggatgcacag ctttataatg gctttttcag tgacgccgat
     1381 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
     1441 acttttgttg ataaacgaat tgaaaagttg ttgttcaatt atcgggcacg caacttcccg
     1501 gggacgctgg attatgccga gcagcaacgc tggctggagc accgccacca ggtcttcacg
     1561 ccagagtttt tgcagggtta tgctgatgaa ttgcagatgc tggtacaaca atatgccgat
     1621 gacaaagaga aagtggcgct gttaaaagca ctttggcagt acgcggaaga gatcgtctaa
     1681 tttgaagccg ccgccgctgg tacggcggag gacaatgttg agtcaggctt tttgaacggt
     1741 gatgctccac gctgcatcgc caatctgctg ataatcggtg atggcatgtc cttcttctgc
     1801 cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc
     1861 gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg
     1921 cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag
     1981 ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc
     2041 cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca
     2101 gtccattacc gatagagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac
     2161 ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg
     2221 ctttggctgc aataaatgcc tccacgaaga tccctaacac caggaaaacg ccccagttaa
     2281 tgtatttcac gtcacccgcg accagaaatt gaagaatatt ggccgttggg gaagtgactc
     2341 ctaacccaaa catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac
     2401 cgatgagtac agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc
     2461 cggttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca
     2521 ccacccagag cgttatcacc agcaaaacgg taaccaacgg ccacacagac aaattaaaag
     2581 tctcagcaat agagttatgt tcagtagtgt agtgctgcaa ggtttgattt aaaccactgg
     2641 cgtgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc
     2701 cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa
     2761 ttcccagccc gaaaagatac ccacctataa cagtaccaag ccacgggaac gccccggctt
     2821 cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt gccgaaattg
     2881 caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac
     2941 cagtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc
     3001 cgcttaatat cattgaaaac ataaattaat taaccagatg aatgttaatg gggaaatttt
     3061 tcatgactgg tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt
     3121 aatgaggatt tcgataaaaa agaataaaaa aaccggagcc aaatgttcat tcgactccgg
     3181 tttattatta gaaaagatga ttatgctacg tcttcgtact gcggaaccgg attacggaag
     3241 ctcttggtga cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc
     3301 attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc
     3361 aggaacagat actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac
     3421 tgcgagatga ccgacaggtt aacaaaggtg aacgccacca gcgcaccaaa gttaatcagc
     3481 gccgttgcca ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg
     3541 atgttcatcg ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac
     3601 acgccgtcac gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg
     3661 gacgccagta cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca
     3721 acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg
     3781 aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc
     3841 aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga cagattgctg
     3901 atgccgtcaa agccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata
     3961 acatgcgcat cgccagacca gaacggacgc gtgctcgcca gcgtaccagc gccttcacct
     4021 tcaaatacgc cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg
     4081 acgattacag tgttgacgtt cgctacggat ttcagactac gcaggttaaa ggcggtcata
     4141 aaggccacca gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa
     4201 tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac
     4261 caacccacca taaagccgac agtcgggcta atggatttct gggcgtaagt gtatgcagag
     4321 ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca
     4381 atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca
     4441 aatgtatcaa acagcgtcat cggctgcata taggctagac ccatcattac aaccggaact
     4501 aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg
     4561 gttattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga
     4621 ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc
     4681 tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctaattaa acgtcggttt
     4741 ttaatcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg
     4801 agaggcttgt cataataatt tttctttgaa tggctgattt tccgtcatca gattttgcat
     4861 aaacaccggc gatcgcacta tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc
     4921 ctgcggtctg ttcattccag ccaatccaga caggttcaaa gagagtttgg gcgaccactt
     4981 tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc
     5041 cgcagccaag gccgctaatt tgcagctcca gtttggtttt aaaatcgaaa acggtaatgg
     5101 cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtatccccca
     5161 ccacaatcgc ccgatatcgt ttaatgatac gacggtttaa cggttcttct tcctgggcta
     5221 acggatgatg aggcgcgacc gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa
     5281 actcactgga agatggtggc tcatgcatcg cgccaacgat aatatccgct cgcccctgag
     5341 tcagggcatc ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat
     5401 gatgttgata aaaagcttca atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca
     5461 cgccaatcac cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt
     5521 ctcgcacggt atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga
     5581 atttagcgcg gtgtccgcta cgatcaagca attgaatatt taggtcgctt tccagcttgt
     5641 gaacggtgta actcagagcg gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac
     5701 tgccctcttt ttctatggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg
     5761 cccccttgca gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccaac ggcattacca
     5821 gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa
     5881 tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggttgcagc cccagtaaac
     5941 gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga
     6001 tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat
     6061 ggactaaatt aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtcccg
     6121 gatgacgtcc cggtccgacc aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta
     6181 aattgtgcag ccagtcttcg agttcttcta atactcgtcc actgttggtt accggattac
     6241 gcggggtagt ttctttaacc gtgccttgcg cgtcgccata gacagatgtt gagctggtaa
     6301 aaataattcg gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta
     6361 aatagaactc atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg
     6421 catccataag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca
     6481 gatagctatc aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc
     6541 cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatacct aaccacccta
     6601 acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc
     6661 tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa
     6721 cgacatgctg caatgagcag tgaatcatca gtaaccgcgt ataccagacg gtgctcctct
     6781 gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca ggggttctgg tttcccctta
     6841 ccttcaaatg gtgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc
     6901 ttatctgttt cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc
     6961 actca
//
LOCUS       DAJTRW010000161         6856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRW010000161 DAJTRW010000000
VERSION     DAJTRW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            53..1543
                     /locus_tag="OO326_003816"
     CDS             53..1543
                     /locus_tag="OO326_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5968017.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1595..2752
                     /locus_tag="OO326_003817"
     CDS             1595..2752
                     /locus_tag="OO326_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5968018.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2718..2840)
                     /locus_tag="OO326_003818"
     CDS             complement(2718..2840)
                     /locus_tag="OO326_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968019.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3037..4722
                     /locus_tag="OO326_003819"
                     /pseudo
     CDS             3037..4722
                     /locus_tag="OO326_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4760..>6856
                     /locus_tag="OO326_003820"
     CDS             4760..>6856
                     /locus_tag="OO326_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5968020.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKAR"
BASE COUNT         2018 a         1494 c         1453 g         1891 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
      721 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
      781 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
      841 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
      901 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
      961 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1021 taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga
     1081 tatcagcatt ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga
     1141 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1201 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1261 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1321 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1381 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1441 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga
     1501 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1561 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1621 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1681 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc
     1741 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     1801 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     1861 tacattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     1921 tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg
     1981 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2041 atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct
     2101 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2161 agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2221 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2281 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2341 tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2401 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2461 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2521 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2581 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2641 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     2701 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     2761 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     2821 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     2881 cgtcagggca ttagcaatac ccttgattat caatagcata accactgaaa attaaaaatt
     2941 tctatatatt tcatattgtt aaatgatgct taaataaatt tcatcagtaa atcattgcca
     3001 tagataatca ttggatggca caatcaattt tcgctaatga taaccattcc cattacgttt
     3061 tgtatgttga tagcaaagta tctctgtctg ctgaagccct tttggctgcg taagaataat
     3121 aaaacttcag tgttgttaat cattattatc ctcgcaatga tccttggcgt ggttaaaatt
     3181 caggtctggt taaacgactg gaataatgat tttttcaatg cgctaagcca gaaagaaacc
     3241 gataagcttt ggcagctcgt tctttggttc cctgccctgc tggggatctt tgtattaata
     3301 tccgcaaaca aaacatggtt aattaaacta ctgactattc gctggcgcga atggttaaca
     3361 gattactacc ttaatcgctg gtttgcagat aaaaactact acttcacgca gatctacggc
     3421 gagcataaaa atacagataa ccctgaccaa cgtatcgctg aagacattct cttactgatc
     3481 aacaaaacgc tcagtctttc atttggcttc atccagtcac ttagcatgct gatcaccttt
     3541 accgttattc tttggcaaag cgcgggtacg ctctcattca ctgttggtgg aacagaatgg
     3601 aatatccagg ggtatatggt ctataccgtc gtgctcattg tgatcggtgg aactttattt
     3661 acccataagg tgggtaaacg tattcgtccg cttaatgtgg aaaaacaacg tagcgaagcg
     3721 acatttcgaa ctaatcttgt gcagcataat aagcaggcag aactgattgc gctaagcaat
     3781 gctgagtctc tacaacgcca ggagttgcgc gacaattttc ataccattaa agagaactgg
     3841 catcgtttaa tgaatcgtca gcggtggctt gattactggc aaaatattta ttcgcgctcg
     3901 cttagcgttc ttccctactt tctgttattg ccgcagttta ttagcgggca gatcaatctg
     3961 ggcggactga tgaaatcgcg ccaggcattt atgctagtat cgaacaattt aagctggttt
     4021 atttataaat atgacgaact tgctgaactg gctgcggtta tcgatcgctt gtatgagttc
     4081 catcaactca ctgaatagcg ccctacgaat aagcctaaaa attgccaaca tgcggtacaa
     4141 gtggctaatg cgagtattcg tacgcctgat aataagatca tattagagaa cttgaacttt
     4201 catgtttcgc caggcaaatg gctattactg aaaggctact ctggtgcggg aaaaaccaca
     4261 ctgcttaaaa cattatccca ctgctggccg tggtttaaag gtgatatttc ttctcctgct
     4321 gacagttggt atgtgtcaca aacaccgtta atcaaaagcg gcttactgaa agagattatt
     4381 tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga gcgaagtact gcatcaggtt
     4441 ggtctgggaa aattggctgc gcgtattcat gaccacgatc gctggggaga tattctttcc
     4501 agcggcgaaa aacaacgtat cgccctggca cgattaattt tacgacgtcc gaaatggata
     4561 tttcttgacg aaactacctc tcatcttgag gaacaagagg ctatacgctt actgcgttta
     4621 gtgcgtgaaa aactacccac aagcggcgtc attatggtta cacatcaacc cggtgtctgg
     4681 aacctggctg atgatatttg tgacattagc acggttttat aacaacaata aaagccctgc
     4741 tgtacggaga tataaataaa tgaagcgagt tcttattcct ggcgtcattt tatgtggcgc
     4801 tgatgtggcg caggccgtcg atgacaaaaa catgtacatg tatttttttg aagagatgac
     4861 ggtctatgct cctgtccctg tacccgtaaa cggcaacacg cattacacca gtgaaagcat
     4921 cgagcgttta ccgaccggga atggcaatat cagcgatctg ctgagaacca accctgcggt
     4981 acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga gatattcgcc ctgagaaaat
     5041 ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg attgacggta ttagtgcaac
     5101 taataacctg aacccagcga atgagtccga tgccagtagt gcaaccaata ttagcgggat
     5161 gtcacagggg tattatcttg atgtcagctt actggacaat gtgacgcttt atgacagttt
     5221 tgtgccggtt gaatttggtc gcttcaatgg cggggtaatt gatgcaaaga tcaaacgctt
     5281 caacgctgat gatagcaagg tgaaattggg ttatcgcact acgcgtttgg actggttaac
     5341 atcgcatatc gatgagaata acaagagcgc atttaatcaa ggttcttcag gaagtaccta
     5401 tttctctcca gattttaaaa agaactttta taccttgtcg tttaatcagg agctggctga
     5461 taactttggc gttaccgccg gtttatcgcg ccgccagtct gatatcaccc gcgcggatta
     5521 tgtttcgaat gacggcattg tcgccggtcg ggcacagtat aaaaacgtta tcgatactgc
     5581 attgagcaaa tttacctggt ttgccagcga ccgctttacc cacgatttaa ccttaaaata
     5641 taccggctcc agccgtgatt ataataccag caccttcccg cagtctgatc gcgaaatggg
     5701 taataaatcc tatggtctgg catgggatat ggatacgcaa ctcgcatggg ccaaactacg
     5761 taccaccgtt ggttgggatc atattagtga ttatacccgt cacgatcatg acatctggta
     5821 caccgaactt tcatgtacat atggtgatat tacagggcgt tgtacccgtg gcggattagg
     5881 acacatttcc caggctgtag ataattacac cttcaaaaca cgcctggact ggcaaaaatt
     5941 cgccgtgggt gatgtttcgc atcaacccta cttcggcgcg gaatacatct attccgatgc
     6001 atggactgaa cgccataacc agtctgaatc ctatgtgatt aatgctgcag gaaagaaaac
     6061 taaccatacc atttaccata aaggtaaagg cagcctggga attgacaact acacgctgta
     6121 tatggcggat cacattagct ggcggaatgt gtcgttaatg cccggtgtgc gttatgacta
     6181 tgacaactat ctgtcaaacc acaatatctc cccgcgcttt atgacggaat gggatatttt
     6241 tgctgatcaa acctcaatga ttaccgccgg ttataaccgt tactatggcg ggaatattct
     6301 tgatatggga ttacgtgata tccgcaatag ctggacggaa tcggtatcag gtaataaaac
     6361 cctgacgcgt tatcagaatt tgaaaacgcc ttataacgat gaactggcaa tgggattgca
     6421 gcaaaaaatc gataagaacg ttattgcacg cgcaagtgaa gcgcatgatc aaatcagcaa
     6481 aagcagtcgt accgacagcg cgactaaaac caccattact gaatataaca acgatggcaa
     6541 aaccaaaacg cattcgttta acctcagttt tgaactggcc gaacccctgc atatccgcca
     6601 ggtagatatt aacccgcaaa ttgtctttag ctatatcaag agcaagggca acttgtcgtt
     6661 aaacaatggt tatgaggaga gcaataccgg tgataaccag gtggtttata acggtaatct
     6721 ggtctcttac gatagcgttc cagtggcaga ttttaataac ccattaaaga tctccttaaa
     6781 catggatttc acgcatcaac cgagcgggtt agtgtgggcg aatacgctgg cctggcaaga
     6841 agcgcgtaaa gctcgc
//
LOCUS       DAJTRW010000162         6803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRW010000162 DAJTRW010000000
VERSION     DAJTRW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            154..333
                     /locus_tag="OO326_003821"
                     /pseudo
     CDS             154..333
                     /locus_tag="OO326_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            858..2036
                     /gene="ltrA"
                     /locus_tag="OO326_003822"
     CDS             858..2036
                     /gene="ltrA"
                     /locus_tag="OO326_003822"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5968021.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2119..2298
                     /locus_tag="OO326_003823"
                     /pseudo
     CDS             2119..2298
                     /locus_tag="OO326_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2328..2810)
                     /locus_tag="OO326_003824"
                     /pseudo
     CDS             complement(2328..2810)
                     /locus_tag="OO326_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3066..4046)
                     /locus_tag="OO326_003825"
     CDS             complement(3066..4046)
                     /locus_tag="OO326_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5968022.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4046..5245)
                     /locus_tag="OO326_003826"
     CDS             complement(4046..5245)
                     /locus_tag="OO326_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5968023.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5567..5905)
                     /locus_tag="OO326_003827"
                     /pseudo
     CDS             complement(5567..5905)
                     /locus_tag="OO326_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5972..6754)
                     /gene="istB"
                     /locus_tag="OO326_003828"
     CDS             complement(5972..6754)
                     /gene="istB"
                     /locus_tag="OO326_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5968024.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1816 a         1580 c         1638 g         1769 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcag ttgctgaact gctggaaccg
      181 ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta aagtccatgc cgatgatatc
      241 cccgttccgg tccaggagcc gggcagcggt aaaacaagga ctgcccggct gtgggtctac
      301 gttcgtgatg accgtaacgc cggttcggaa attgcagcat aagttatcca tgctctgagg
      361 tgggtagagg tgggccctcc ggtcagggtt tacaggagca cagaccaaac gacccggtgc
      421 tgcttcattc taaggaatga ggtggtgagc gactcgggaa aaggcagaga tctactgtca
      481 cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa atcttagatg
      541 atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc tgcttactga
      601 ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc agggaacgtg
      661 agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag tagtgatgaa
      721 agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag aagacacaac
      781 ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa aagcactggt
      841 ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc gacgggcaga
      901 ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg aaccggctat
      961 gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag tcgaatggca
     1021 aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga gcgataaaac
     1081 ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt taccgaccag
     1141 gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt tatagctgga
     1201 tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg gtgctcaaag
     1261 ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc tggatggaag
     1321 cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc actccgcagg
     1381 gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt gatttgtgga
     1441 tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt gtagtacatt
     1501 gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc ttctcggaag
     1561 tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg tttaaaagga
     1621 gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt acgctgaaga
     1681 atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca gcaatgcgca
     1741 aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg
     1801 attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct
     1861 ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga
     1921 tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga
     1981 aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa
     2041 gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct
     2101 ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac
     2161 tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc
     2221 attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt
     2281 gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat
     2341 tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt
     2401 atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc
     2461 tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct
     2521 attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt
     2581 gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat
     2641 gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca gttaagcagt
     2701 tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga
     2761 taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc
     2821 ttacttttgt tgttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg
     2881 ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa
     2941 gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa
     3001 aagcaaaatt tcttttaaaa cagccaatta aaaactggcg agttgctggc gaaaaacttg
     3061 aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg atctaactta
     3121 tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt
     3181 tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc
     3241 tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt
     3301 ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata
     3361 ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc
     3421 agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct
     3481 ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt
     3541 tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt
     3601 tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa
     3661 acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct
     3721 gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat
     3781 ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg
     3841 gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtatc cactttgtca
     3901 tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga
     3961 actggagccg atatttcttc agtattattc aggatatttg tattcagcgt acggccaata
     4021 gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa
     4081 aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc
     4141 caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa
     4201 aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca
     4261 ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg
     4321 cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag
     4381 ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga
     4441 tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa
     4501 ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt
     4561 tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt
     4621 cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt
     4681 ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca
     4741 gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa
     4801 ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta
     4861 cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat
     4921 atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa
     4981 tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat
     5041 agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa
     5101 gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct
     5161 tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct
     5221 tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta
     5281 caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta
     5341 aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg
     5401 cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg
     5461 acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg
     5521 acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt
     5581 gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga
     5641 ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgtgcaatac
     5701 gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc
     5761 cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc
     5821 gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt
     5881 tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc
     5941 tccaccaacg gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc
     6001 cggcctttcg tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt
     6061 ggtgtaagat acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat
     6121 cccactgccc gaacggtaga ttggatgtca ggatcattgc gctcttttcg taacgtttag
     6181 cgatgacctg gaagaacagt tttgcttctt cctgactgaa cggcagatag cctatttcat
     6241 caatgatgag caggcggggg gccattactc cacgctgaag cgtcgtttta taacggccct
     6301 gacgttgtgc cgtagataac tgaagtaaca gatctgctgc tgttgtgaag cgaactttga
     6361 tacctacacg gactgcttca tagcccatcg ctattgccag atgggttttc cccacacctg
     6421 atggccccag taatacgata ttttcattac gttctatgaa gctgagtgag cgtaacgact
     6481 ggagttgctt ctgcggtgct ccggtggcga atgtgaagtc atactcttcg aacgttttca
     6541 ccgccgggaa ggctgccatt cgggtataca tcgcctgttt acgttgatga cgtgccagtt
     6601 tttcttcatg aagcagatgc tccaggaagt ccatataact ccattcctgg tctactgcct
     6661 gttgtgacag cgcaggcgct gcgcttataa ggctttccag ttgcaactgc ccggcgagcg
     6721 ccatcagtcg ttgatgttgc agttccatca tcacgccact cctctgcaga atgagtcgta
     6781 gatggagagt ggatgatgct ggg
//
LOCUS       DAJTRW010000163         6525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRW010000163 DAJTRW010000000
VERSION     DAJTRW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            441..1829
                     /gene="narU"
                     /locus_tag="OO326_003829"
     CDS             441..1829
                     /gene="narU"
                     /locus_tag="OO326_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5968025.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1911..5651
                     /gene="narZ"
                     /locus_tag="OO326_003830"
                     /pseudo
     CDS             1911..5651
                     /gene="narZ"
                     /locus_tag="OO326_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5648..>6525
                     /gene="narH"
                     /locus_tag="OO326_003831"
     CDS             5648..>6525
                     /gene="narH"
                     /locus_tag="OO326_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5968026.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1560 a         1715 c         1690 g         1560 t
ORIGIN      
        1 accttagatg aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga
       61 gaggaaccga tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga
      121 ataaatcatc actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac
      181 cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt
      241 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      301 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      361 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      421 gtgtgcatat gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      481 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      541 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      601 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      661 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      721 tgcctatatt cggtggacgc cgatggccgg tttttagtac tgcaatcctg attattcctt
      781 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
      841 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
      901 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
      961 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1021 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1081 catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1141 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     1201 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1261 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1321 ttggcccatt tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1381 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1441 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1501 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1561 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1621 gagaagccgt caccgaaacg gcggcaacgc tgggctttat ctcagccatt ggcgcagtgg
     1681 gcggcttttt tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg
     1741 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     1801 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accatgtcgc
     1861 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     1921 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     1981 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2041 aaatcgtgcg ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg
     2101 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2161 acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt
     2221 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg
     2281 aagccctcaa acaacacagc gatccggtac tggcgtgggc atcgattatg aatgatccgc
     2341 aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2401 aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg
     2461 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa
     2521 cgcgttatct gtcgctgctt ggcggcacct gttaaagttt ctacgactgg tattgcgacc
     2581 tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact
     2641 ggtataactc cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg
     2701 acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg
     2761 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     2821 gcgccctggc gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca
     2881 gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg
     2941 agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg
     3001 atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg
     3061 aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3121 tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3181 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3241 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3301 atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg
     3361 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3421 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3481 aatttgccga taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg
     3541 ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct
     3601 gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc
     3661 gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc
     3721 agatgaacag cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     3781 ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg
     3841 atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta
     3901 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     3961 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4021 atcatccgcg taatttgttt gtctggcgct ctaacctgct tgactcctcc ggcaaaggcc
     4081 acgagtatat acagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4141 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4201 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4261 tcgttctgcc caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc
     4321 catttattca tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg
     4381 aaatctacaa aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag
     4441 aaaccgacgt ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt
     4501 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga
     4561 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4621 cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa
     4681 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4741 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     4801 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     4861 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     4921 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg
     4981 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5041 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5101 ctatcgacac ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa
     5161 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5221 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg
     5281 cccgagaact gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc
     5341 tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg
     5401 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5461 ataactcggt cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5521 tggcctgggg ctttaactac tacggcaccg tcggttcgaa ccgcgacgaa ttcatcatga
     5581 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5641 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg
     5701 ctgccatacc tgttcggtga cctgtaacaa cgtctggacc ggacgcgaag gcatggagta
     5761 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     5821 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct
     5881 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat
     5941 tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagca caccggaagg
     6001 caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt
     6061 gatctggggc ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga
     6121 ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat
     6181 gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag
     6241 cggcgctatc tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg
     6301 tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag
     6361 cggcaagtca gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt
     6421 gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga
     6481 ccgcatcgag gaagcgtcga gcaccgagcg cgaagttgac ctcta
//
LOCUS       DAJTRW010000164         6471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRW010000164 DAJTRW010000000
VERSION     DAJTRW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            240..2672
                     /locus_tag="OO326_003832"
     CDS             240..2672
                     /locus_tag="OO326_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5968027.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO326_003833"
     CDS             2739..4208
                     /locus_tag="OO326_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5968028.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO326_003834"
     CDS             4208..5869
                     /locus_tag="OO326_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5968029.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO326_003835"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO326_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5968030.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT         1914 a         1524 c         1496 g         1537 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt c
//
LOCUS       DAJTRW010000165         6341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRW010000165 DAJTRW010000000
VERSION     DAJTRW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..117
                     /locus_tag="OO326_003836"
     CDS             <1..117
                     /locus_tag="OO326_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5968031.1"
                     /translation="MQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(456..1226)
                     /locus_tag="OO326_003837"
     CDS             complement(456..1226)
                     /locus_tag="OO326_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968032.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1271..1705)
                     /locus_tag="OO326_003838"
                     /pseudo
     CDS             complement(1271..1705)
                     /locus_tag="OO326_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1717..1938)
                     /locus_tag="OO326_003839"
     CDS             complement(1717..1938)
                     /locus_tag="OO326_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968033.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1939..2622)
                     /locus_tag="OO326_003840"
     CDS             complement(1939..2622)
                     /locus_tag="OO326_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5968034.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2686..2997)
                     /locus_tag="OO326_003841"
     CDS             complement(2686..2997)
                     /locus_tag="OO326_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968035.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(2994..3896)
                     /locus_tag="OO326_003842"
     CDS             complement(2994..3896)
                     /locus_tag="OO326_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5968036.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4336..5295
                     /locus_tag="OO326_003843"
     CDS             4336..5295
                     /locus_tag="OO326_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5968037.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5295..5690
                     /locus_tag="OO326_003844"
     CDS             5295..5690
                     /locus_tag="OO326_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5968038.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5873..6253
                     /locus_tag="OO326_003845"
     CDS             5873..6253
                     /locus_tag="OO326_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5968039.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1736 a         1651 c         1531 g         1423 t
ORIGIN      
        1 atgcagcttg aacgccagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttgc ctgcgctctg gcaaataaac tggcccgaat tgcctaggca
      121 atcactactc agcaaaaaga atatcaggcc tgatatatag atgctgacaa aactcaatta
      181 gcttattgca aacaataaga tacaagttac cgtctggttt tgcgaagatt gattattgat
      241 gacatgaacg gcaaatcgac ctgattaaaa acctaacagg ggaaaatggc tcactgaagc
      301 cgaggggatt tttaggatta tcaggtgcgg aactcatcga ggcgcgggca tcccttgccc
      361 acagagacgc cggatagatt taagcaagcc caccattaat caaaaatcag tgttgcaaaa
      421 acgggggtga ccatagattt tccccgccat cgtcatcatt tccggatttt tgccagggca
      481 tccgccatgc tgcggatttc ggcccgggct tcccggacaa cttcccgcag aacaccacgg
      541 tcaaaccacc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
      601 gacaggatat gctcaataca atcgctgacc agatacattt ccaccgccga aaaacgtgcg
      661 ccccggttca ggatggcatc gagcaccacg ggcaggtttt catcgtcttc atcgagccag
      721 caggggtgat cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagcaac
      781 gtctgaccgc ttgtggtcag cgccgcccgg atacgccgcg catcttctgc cccccggcaa
      841 ctgacactgc atactgcatc gcgcaccgca tcaagaactg ccctgatttc gtcttcggtc
      901 agccattcac cggcatacgg gaaagacacc tgcgttccgt ccgccggaag taccggcagc
      961 gactgccact gactgccgga cagaacaagt tccgctccgg ttccggtcag tttctgaatg
     1021 gcggaggcca ggtgcggcgg cagcacgcca tcgtggcggt taacgagtaa cgtcgccagc
     1081 cggggcgcat ccagccgcac gtaaatgctg atccagcccg gatagttcgg gttacattgg
     1141 tcaacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtgacgacaa
     1201 atcagagcat tcagcgtttc attcataatt catgaactcc ctgtatcgcc gcccacaggc
     1261 ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag caggctgcgg gcctgatcaa
     1321 ccatcgcctg tcggcgggta acgtattccg ggagggggaa accgtgatcc cgggcagccc
     1381 attcacgttt ccacagtgcc tgttcagcga tctgagtcag ggtctccagc agttcagcag
     1441 ggacggtcac ctggcggttt tcgcgcacgt tgctgataat ctccatcgcc gcggcggaag
     1501 aaataccgga ctcaatccgc tagtcttccg ggatgtcctc caggtgcgcc aggacggtcc
     1561 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctga atctcttccg
     1621 gggaccagat aaccagcatc agcggcgtgt ctcccgaata ctttgctgcc agcgcccggc
     1681 atagcgtttc gcatgttccg tacatactct ctcccctcaa tctttgaagc aggaatccag
     1741 ttccagaatt tcggtatatg cctccatcaa gaggcggttt gcgcgaaaca acgtctgacg
     1801 caccagacgt tccggcaggg tgtcagcatt aaagctgtac cgccgctcca tctcattcat
     1861 ggttgtacgc aggtcgcaca tggcattggc cacctccgcc acttcggcgc gaagtttttc
     1921 gtgtccggca tagttcattt acgcagcctc cggcataaac cagtcatcat tcgcggcccc
     1981 ctgctgcatg gcccgctgta cggcagccag tcgttgctgt ccggcacggt gatactgctc
     2041 aagcagctcg ataccgatat accggcgtcc ggactggagg gcggcgacgc aggttgaacc
     2101 gctgcctgca aacgggtcca gcactatcgc attcgggtgt gtgaagctct caatcagcgg
     2161 ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg ttgcccgaat atttccagcc
     2221 caacacgtcc ggcagcgggt tttgtggcag acgtggacgg ccttttgcca ggatgtaggc
     2281 gcattcgtgg cgatagccca catatgcggc cttcgatgtg tagtttttgg tgaagaccag
     2341 gtgaccaaca acgctgaatc ccgcattttt ccaggcagac ataaagcgat cgacgcggtt
     2401 ccagccatag aagctcacca tcagcgcgtc ttttttgagt acgcggtaca tttcattaca
     2461 ggccggttgc agccattcat cagttttatc accagcaatg gtacgcccct gacggtcacg
     2521 aaaaccgacg agatatggcg gatcggtgag gatgaaatca atggcgttat caggaatacg
     2581 ggccataaca tcgatgcagt taccgaggac aaaacgggac ataacagatc cttctcttac
     2641 agagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcatc gcttccggta
     2701 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tttgacagca aggttatgcc
     2761 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac gggctggcgt acactgtcac
     2821 cgacaccagc cgggagcggt aaaagcgccc gtcccgtgcc cgccggacgc gggacactga
     2881 ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag gagcacggcg
     2941 acatcctttg tcggaaaaga aacgggcggt tgcccgcccg tctccgtcat acttcatccg
     3001 ccataatgcg ccacattgtc gactatccac gcaggtccgg tgacttccgg ctgctcatcg
     3061 tcatctgtcg gggaagacca ttcaagttcc ccccacagca cattgacgag ctctccacgc
     3121 tcatacaact gccagccaca gaaatcacag ccctgttcgg cataccagtg ctccagcgtg
     3181 cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt ccggctgaca ccacggagtg
     3241 tcgaaatcca cctgaatgaa gttttctccc tgactgctga tattcaggtc atatccacaa
     3301 ggccacttca cgccataccg ttccgtatac cagtgatatg cagaaggaac accgttcaga
     3361 acaccaccat taaagccgtt cacttcaaca tccaggcggg tcggcagaac cgtcagcagg
     3421 tcaaacggca tggttttttc cggcactacg ttgtcacaca gacggttcca ccacaccgat
     3481 acatcctcgt taccccatat gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg
     3541 atggtttccc tggcatcatc cggtaatcct tcccaacgac gctggccagt accgctctgt
     3601 aaccagagtt catgcagcat caggcagttt tgttcatcca gcaacacacc gttctgcagg
     3661 tgtgtcagcc agcgggtgaa cgcgatattt tccgtcgata cagcaccacg tccggcagcc
     3721 gtcactccgg ggcactgttc agagcggata ttttcagtga tctgcagaag tccggcactt
     3781 ccggccagaa acagctgaat accttcattt gtggcgcggc gataaagcgg tgtgactgca
     3841 ccgctggcca gtcgtttaat ctcagcgatc tgtgccggct caccaggaaa atacatacga
     3901 ttcgagcacc agtttggcat gggaattctc ctctcacatg cggcctccat caggccgtgc
     3961 cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt
     4021 ggaggaggaa tgacgaaacg tgaccgggtg aggaggccgg cgacagcgac acacttgcat
     4081 cggatgcagc ccggttaacg tgccggcacg gcctggttaa atagtatccg tctcagttcg
     4141 tggcgcaaaa tgagaaacca ataagaactc aaaatgaacc acaacaagaa cataaagaga
     4201 ctctaaaaga gcaacaccgt ttgacataga ctcaaaatga gtcacaataa gaaacaataa
     4261 aaaactcaaa aagatgcacc gacataaagg atgctcaatt tgagataaac acaaactcaa
     4321 aacgaggcaa aaagaatgct aaaagtatca tgcgatgatg gttcaactaa cgtaaaactg
     4381 gcctggcttg aagatggcga ggtaagaacc agtctgtcag gaaacagctt taaagaaggc
     4441 tggaacccgg gactgtttaa tgccggcaaa gtttacaatt acgtcgtgga tgaaaaaaaa
     4501 tacacctatg atttgggaag cactgctgtc ataggtacaa cacatgtcag ttatcaatac
     4561 agcaccacca atctgcttgc catacatcat gcactgctga catccggcct tcagccacag
     4621 gatgtagaac tgacagtaac attacctgtg accgaattct ttgataatga caatcaacca
     4681 aatgaagaaa gaatagaaag aaaaaaggca aatgttctcc gtgagatatc tctgaataaa
     4741 ggggaaacat ttaaaattaa aaaagttaat gtaatgcctg aatctttgcc agctgcattt
     4801 gaatcactaa aaaaagataa agtaaacaaa cttgaacgca gcctgattat cgatcttgga
     4861 ggaaccactc tggattgtgg tttaattctc ggtgcatttg aaggcatttc tgaaatccgt
     4921 ggctattcag aaatcgggac atcccgcatc actcataccg tcatgaatgc gctgacgaaa
     4981 gcctccacac catgtaatta ttttattgct gatgagttaa taaaaaacag acacgataat
     5041 gaatatctcc agacattaat aaatgatgta gctgaaataa aaaacatttc tcatgtaata
     5101 gacagagaag tgaaatctct ggcagaaagc atcaggcagg aaatttcaac attctcagga
     5161 atgaaccgaa tctatctcac tggcggagga gccgagctta tttaccctca cataaagcaa
     5221 tatttcccga atctgaaagt gaacaaggtg gatgaacctc agtttgcatt ggtgaaagca
     5281 atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag aaagaaagtt gtggcatacc
     5341 tgcacccggc actttatccg caggacaacc tgacacaaca aacaatagac tctctcccag
     5401 tacaaatgcg gggagatttc tacagacaat cccttatttg cggagcagct ctttattctg
     5461 ttgccccccg actgttaacg ctaatcagcg tttttttcag tgagaaaatc actgcagaaa
     5521 atctggtgaa acttatagaa caaacaacag gatacacatc cacatcaatt gatataagtg
     5581 tgttaaaaaa catcatagag gcatcttcag aaaacaaatc agagagcatc acttcaaaag
     5641 atgatttcga agaacaaacc agacgcaatc tttctatgtt aaaaaaataa taaaagaaga
     5701 gcaggttcag tcaattacct gctcttcttt tatacatact aaaaaatcag atttttacga
     5761 atattcagat tatcataaaa aatgcaacag acaggaatgc aataattttg cattatctcg
     5821 ttgcttgtaa tgcaaatatg cattattgtc agtgtgtgac aataacagag agatgacgat
     5881 ggcgaccatt aacgcacgta ttgatgatga tataaagaac caagcagatg aagtgctgaa
     5941 gctgatgaat atcagtcaaa ctcaggctat tgcagctttt tatcagtata ttactgaaca
     6001 aaagaaactg ccatttgtga tcacctcaat tgtaaaaacg ccgcatgatt tattacgtga
     6061 aagtacagac atgttagctg aagctcttgc agtgatttcc aatctacagg tgtggactga
     6121 acagcaggat gggattggaa aagcaaagct aatggagtat taccgccgcc tcgacgcact
     6181 gtactgttgt gcaaaagaga aaataggtct tctttcagat aaccgggatg ctgaactagg
     6241 ctgtgtccct taatcatctg agttatagta actgtctgtt tacacaacat attgaactat
     6301 ggctcgctac gaccttcccg atgaggcatg gaccatcatc a
//
LOCUS       DAJTRW010000166         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRW010000166 DAJTRW010000000
VERSION     DAJTRW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..312
                     /locus_tag="OO326_003846"
     CDS             <1..312
                     /locus_tag="OO326_003846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5968040.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO326_003847"
     CDS             complement(388..852)
                     /locus_tag="OO326_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5968041.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO326_003848"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO326_003848"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5968042.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO326_003849"
     CDS             complement(1721..2410)
                     /locus_tag="OO326_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5968043.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO326_003850"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO326_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5968044.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO326_003851"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO326_003851"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5968045.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO326_003852"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO326_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5968046.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1585 a         1695 c         1552 g         1423 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag aatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 acaatgggta atgct
//
LOCUS       DAJTRW010000167         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRW010000167 DAJTRW010000000
VERSION     DAJTRW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO326_003853"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO326_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5968047.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO326_003854"
     CDS             2089..2238
                     /locus_tag="OO326_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968048.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO326_003855"
     CDS             complement(2983..3423)
                     /locus_tag="OO326_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5968049.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO326_003856"
     CDS             3641..4030
                     /locus_tag="OO326_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968050.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO326_003857"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO326_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO326_003858"
     CDS             complement(4585..5421)
                     /locus_tag="OO326_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5968051.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO326_003859"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO326_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5968052.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1432 a         1617 c         1641 g         1510 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRW010000168         6161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRW010000168 DAJTRW010000000
VERSION     DAJTRW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..430
                     /locus_tag="OO326_003860"
     CDS             <1..430
                     /locus_tag="OO326_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968053.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO326_003861"
     CDS             550..723
                     /locus_tag="OO326_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5968054.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="OO326_003862"
     CDS             823..1644
                     /locus_tag="OO326_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5968055.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="OO326_003863"
     CDS             1986..2459
                     /locus_tag="OO326_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5968056.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2475..2951
                     /locus_tag="OO326_003864"
     CDS             2475..2951
                     /locus_tag="OO326_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5968057.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3014..3235
                     /locus_tag="OO326_003865"
     CDS             3014..3235
                     /locus_tag="OO326_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5968058.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3398..3772
                     /locus_tag="OO326_003866"
     CDS             3398..3772
                     /locus_tag="OO326_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5968059.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3819..4193
                     /locus_tag="OO326_003867"
     CDS             3819..4193
                     /locus_tag="OO326_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5968060.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4190..4681
                     /locus_tag="OO326_003868"
     CDS             4190..4681
                     /locus_tag="OO326_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968061.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            4693..4890
                     /locus_tag="OO326_003869"
     CDS             4693..4890
                     /locus_tag="OO326_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968062.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4975..5535
                     /locus_tag="OO326_003870"
                     /pseudo
     CDS             4975..5535
                     /locus_tag="OO326_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            5607..>6161
                     /locus_tag="OO326_003871"
     CDS             5607..>6161
                     /locus_tag="OO326_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5968063.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT         1511 a         1655 c         1551 g         1444 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca ccgcttcggc ctgtgcaccg gagactggcc
     2041 tgcaacaact ggttgccact cccgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 attttggcct cattccacag tgggtcaccc tggaaccccg tgtcttcggc tggatggacc
     2161 gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg aaacatgacg cccggcgagc
     2521 gcagtctcat tctgcgggcc ctgcaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     2641 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     2701 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     2761 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     2821 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     2881 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     2941 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     3001 gaggtatcac atcatgagaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
     3061 tcctgcatcc cgtctttttc cgttctgtac cggtaagtat cgctggcacg gcagtgctga
     3121 agcgtatact ggtcgtgaag tgcaggatat tcccggcgtt cttgccgtgt ttgcagaacg
     3181 ccgtaaggac agttttggcc cgtatgtccg gctgatgagt gtcaccctga actgatgttc
     3241 cgtcacccgg gaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccatt
     3301 ctcatcaatg catacatctt caccgaaacc ggtcagcgcg ccggctgacc tgtcatcgtt
     3361 aaaccattct taaccacttt tcagagagga ttttatcgtg tcagacaaac tctccgggat
     3421 aactcatccc gatgacaatc acgaccgccc ctggtggggg cttccctgca cagtgaggcc
     3481 ctgttttggc gctcgtctgg tgcaagaggg taaccgcctg tattaccttg ccgaccgcgc
     3541 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
     3601 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gatatcagca
     3661 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg
     3721 ttacgtttat ctggctgttt atccgacacc agcacccgca accacctcat aaccccgaag
     3781 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
     3841 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
     3901 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgtc gatgaacgtg
     3961 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcagttaat tttctcgtgg
     4021 aaaaatacgc gctggtgcgt accgaccagc cgggattcag cacctgtccc cgctctcagt
     4081 taataaacag tattgatatc ctccgggctc gccgggcgac cggcctgatg acccgcgaca
     4141 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgaaact
     4201 ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaacatag ggcgcattgt
     4261 cgttgacgtc gatggtattg agctcgctga actgattaac atggtctgcg ataacggcta
     4321 ctcccttcgt gttgttgatg aatctgacca gacctcagca gaatgcacgc caccatttgc
     4381 tacccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca acgcctggtt
     4441 gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc attttacagg
     4501 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     4561 ggggctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt atggcgtcag
     4621 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     4681 accaggaaca acatgaaatc attaaccacg gaaacagcgc tggatattct gattgcgtgg
     4741 ctgcaggaca atatcgactg cggatcggga attatctttg acaacgatga ggataaaacg
     4801 gattcagcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg taccctgcgt
     4861 catctgcagc ttctgcacca gaaccggtga agctcactca ccagacttca ttcctctgac
     4921 gccagcctgc acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     4981 gatatcacaa tctcccgtcc ggaagtggtc aacgggcata cggacgttat ctgctcaacc
     5041 agcattcgcc acattctggc tgtacgaaag agtacactgt tgcaaatcga cacacttatc
     5101 cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg gaggtaaaac tgccccggac
     5161 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc cgaaacagca
     5221 atgaaagcca ttacccggaa tcttgatcgc gaaatctggc gtgacctgat gcaacgttcc
     5281 gggatgctgt cattaatgga tacgcaggcc cgtgatacat ggtaccggtc actggagtac
     5341 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     5401 aataaggatg aggtgtttga gcgaggggtg atcaacgtct tcagagggct gaactggaat
     5461 tacaaaacca attgcccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     5521 tgggaccggt ggggataatg atatggtcac ccccacctta acagcagtac gctgttaagg
     5581 taaatccctt taacggagag accatcatgc agaacatcac acttattggt atcgatctcg
     5641 gaaagcactc cttccacatt cattgtcaag acaagtctgg taaggctctt ctgcgtaaaa
     5701 aatttacccg tactaaactg atgcagtttc tggcgacatg tccgccctca gttgttgtaa
     5761 tggaagcctg tgcgggtgca cactttatgg ctcgctgtat ctctgatttt gggcatgaaa
     5821 ctaagctgat ttctccacag ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg
     5881 ttgatgcaga agccatatgc gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
     5941 gaactgagac ccagcaggcc atgagggcgt tacaccgggt cagagaatcg ctcatcagag
     6001 ataaggtgaa aacaaccaac cagatccacg gtttcttgct ggaatttgga atcagcctgc
     6061 cgataggtga tgctgtaata aaacggttgt ctctcgtgtt ggctgaacac gaaatccccg
     6121 aatatctgag ccgccttttg gtgaggttgc atacccacta c
//
LOCUS       DAJTRW010000169         6152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRW010000169 DAJTRW010000000
VERSION     DAJTRW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..172
                     /locus_tag="OO326_003872"
     CDS             <1..172
                     /locus_tag="OO326_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968064.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            277..1629
                     /gene="celF"
                     /locus_tag="OO326_003873"
                     /gene_synonym="chbF"
     CDS             277..1629
                     /gene="celF"
                     /locus_tag="OO326_003873"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5968065.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1642..2400
                     /gene="chbG"
                     /locus_tag="OO326_003874"
     CDS             1642..2400
                     /gene="chbG"
                     /locus_tag="OO326_003874"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5968066.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2447..4708)
                     /gene="katE"
                     /locus_tag="OO326_003875"
     CDS             complement(2447..4708)
                     /gene="katE"
                     /locus_tag="OO326_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5968067.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4891..5154
                     /gene="cedA"
                     /locus_tag="OO326_003876"
     CDS             4891..5154
                     /gene="cedA"
                     /locus_tag="OO326_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5968068.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5431..6152
                     /locus_tag="OO326_003877"
                     /pseudo
     CDS             5431..6152
                     /locus_tag="OO326_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1576 a         1310 c         1558 g         1708 t
ORIGIN      
        1 gatccgtatt aattttgcaa aaaaacaact ggaaatgacc aactattccg tgacggatat
       61 tgcgtttgag gccggttata gtagcccgag tttgtttatt aaaacgttta agaaattaac
      121 gtcctttacg cctaagagat atcgtaagaa attgactgaa tttaatcagt aagtcgttat
      181 acctgacaat tcacatatct gtcctgttgc tggattattt atgtccgggg gggcagatat
      241 gccagatatc agtattctgt actgaaggga gaaattatga gccagaaatt aaaagtcgtc
      301 actattggtg gcgggagcag ctataccccg gagttactgg aaggatttat taagcgttat
      361 cacgaattgc cggtcagcga attatggctg gtggatgtcg agggtggcaa agctaaactg
      421 gatattattt tcgatctctg ccaacggatg attgataacg ctggcgtccc gatgaagctt
      481 tataaaacgc tggattgccg cgaagcattg aaagatgctg atttcgttac tacccaactg
      541 cgcgttggcc aattaccggc gcgtgaactg gatgaacgta ttccattaag tcatggttat
      601 cttggtcagg aaaccaacgg cgcgggcggt ttgtttaaag gtctgcgtac cattccggtg
      661 atttttgaca tcgtaaaaga tgtcgaagaa ctttgtccga atgcatgggt gattaacttc
      721 actaacccgg cgggaatggt tactgaagcc gtttatcgtc ataccggatt taaacgcttt
      781 atcggcgtgt gtaatattcc gatcggcatg aagatgttta ttcacgatgt tctgatgctg
      841 aaagacagcg atgatttatc tatcgatttg tttggcctca accatatggt gttcattaag
      901 gatgtgctgg taaatggcaa gtcgcgcttt gccgaattgc ttgatggtgt ggcatccggg
      961 cagttaaaag tatctggcgt taaaaatatt ttcgatctgc catttagcga aggcttaatt
     1021 cgttctctga atctgttgcc gtgttcttat ttgctttatt acttcaagca aaaagagatg
     1081 ctggctattg aaatgggcga atactacaaa ggcggcgcac gagcgcaggt agtacagaaa
     1141 gtcgagaaac aactttttga gctgtataaa aacccggagt tgaaagttaa gccgaaagaa
     1201 ctggaacagc gcggtggggc ttattactct gatgcagcgt gcgaagtgat caacgctatc
     1261 tacaacgaca agcaagctga acattacgtt aatatcccgc atcatgggca tattgataat
     1321 attccggcag actgggcggt agaaatgacc tgtaagctgg ggcgcgatgg cgcgacgcca
     1381 catccgcgca ttacgcattt cgatgataaa gtgatggggc tgattcacac cattaaaggc
     1441 ttcgagattg ctgccagcaa cgccgcactt agcggagaat ttaacgatgt gttactggcg
     1501 ctaaacctta gtccgttggt gcattccgat cgcgatgctg agctgctggc acgcgagatg
     1561 attctggcgc acgagaaatg gctgccaaat tttgccgact gcatcgcaga gcttaaaaaa
     1621 gcacattaac cgaggctgat tatggaacgc ttactgattg ttaatgccga tgattttggc
     1681 ttaagcaaag gtcagaacta cggcattatc gaggcctgtc gcaatgggat tgtcacatcg
     1741 acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag tcgtgatgaa
     1801 ccgagtctgg ccatagggat gcactttgtc cttactatgg gcaagccact gacagctatg
     1861 ccggggttaa cccgcgatgg tgtgctggga aaatggatct ggcagttggc agaagaagat
     1921 gctttaccgc tggaagaaat tactcaggag cttgccagtc agtatttgcg tttcattgag
     1981 ctatttggac gcaaacctac gcatcttgat agccatcatc atgtgcatat gttcccgcag
     2041 attttcccga ttgttgccag gtttgcggct gaagagggga ttgcgttgcg catcgaccgt
     2101 cagccgctat ctaacgctgg tgatttaccg gctaatctgc gcagttcgca gggattcagt
     2161 agcgcgtttt atggtgaaga gattagcgag gcactgttcc tgcaagtgct ggatgacgca
     2221 agccatcggg gtgatccttc gctggaggtg atgtgtcatc cggcgtttat cgataatact
     2281 atccgtcaga gtgcttactg tttcccacgg ttaacggagc tggaagtgct gacatcagcg
     2341 tcgttgaaat atgcaattgc tgagcgtggt tatcggttgg gtagttatct tgatgtgtaa
     2401 gtaaggtggt aaggtcactc cccaggggac gtgaccttat ccatatttac gccggaattt
     2461 tgtcaatctt aggaatgcgt gaccacacgc ggtgttctgc catcagcgtt aacagttcat
     2521 ccataaaact accgtcggtg ctgtcagctt ccacaatccc ttcttcaccc tggtcagcta
     2581 ccttgattgt tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt
     2641 tgtaggcttc catcagatag tagttggcat cgccgttgtc agcgatatcc gcgatattgc
     2701 cgcaagggac aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta
     2761 taggcaacac cgtaccgtca tccgcagtca cttcacccat tcgggagtag agcagtttgg
     2821 catgaacgcc tttggccttc atggccttga gaatggccag aaggtctgcc gatctcactt
     2881 catcattaag taaaatcgct accacgcgac ctttcacatc accgtcagga atggcgtaca
     2941 aacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg atattcagct
     3001 ggtcgtcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa
     3061 tatgcgccag ctggtcaaca acgcgctcac gaatatacgg acgaacgact ttgcttaact
     3121 caaaactgaa accatcgaca atatggcgct gctcgaatgg cgtctgactt agccagaaca
     3181 gacgcggatg ggaataatat tcgccaaacg atgggctgcg ctcgcgaact ttattgcctt
     3241 ccacgcgctc ttggtatgat tcaaaaccgc cgcgtttcgg ccccggtggt gtttcgcgcg
     3301 gccagttatc gttgatcgag ttcggttcat aattcgccgg gttagtgtcg atccccatac
     3361 gatgcatgcc gtcacgctgg aaattatggt aagggcaggt cgggcggtta atcggaatct
     3421 catggaaatt cggcccacca agacgactga tttgtgtatc ggtataggag aacaaacgtc
     3481 cctgcaacag gggatcgttg gtgaagtcca gaccaggaac aatatgacct ggatggaatg
     3541 ctgcttgttc gttttcggca aagaagttat ccggattgcg attgagcacc attttgccga
     3601 cacgctgaac gggcaccagt tcttccggga tcagtttggt tggatcgaga agatcgaagt
     3661 cgaacttgaa ttcatcttct tcaggaatca actggaagcc cagttcgtat tccggaaaat
     3721 cgcctgcttc aatggcttcc cacaactcgc ggcggtggaa gtccgggtca cgtccggtga
     3781 gtttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc cagtggaaac
     3841 gtacaaacgt tgccttccct tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt
     3901 ccatggtgcg gtaactgcgg gggatgccgc gatccgacat cgcccacatc acgttgtgca
     3961 gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcactt tgcccttgtg
     4021 gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat ttatgcgcat
     4081 cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc tcttcagtat
     4141 agaacttggt ggcaaaacca cggatatcac gcacggtatc ggcagagcca gcgccaccct
     4201 gaacggtaga gaacctaaca aatactgggg tgattttgtt cggatctgag aggaaatccg
     4261 ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct gctgagccgc
     4321 gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca
     4381 gaataaaatc ttccagcagc gttggaccac ggttaccggc acgcagtgag ttttgatcat
     4441 cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt
     4501 cttccagaga attaagtttt tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg
     4561 taggttgtgc acctggcggt gttggttcag ccgctggacg atgagatccg tcctcaggtg
     4621 ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat
     4681 gtgggttctt ttcgttatgt tgcgacattg aactcgtctc cttaatttat tactgaaagg
     4741 gccgctgttt tggtcaaaaa actctctaac tatagaccac cagccagatc ccgcttcgga
     4801 gactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag gggctaaatc
     4861 ggctaaacta cagtttttct aatttttaat atgcgtttag tgaagccagt aatgaagaaa
     4921 ccgctccgcc agcaaaaccg ccagattatt agctatgtcc cacgcacgga acccgcgccg
     4981 ccagaacatg cgataaagat ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat
     5041 gttgcgtttg tactgatggg agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa
     5101 tctgcccaac gttgggcaaa tcagatccgc caggaagggg aagtgactga gtaaggatta
     5161 catacctccc ggttttgccg ggagttacag gacctgaaat tctgcatttc gttgctacat
     5221 tcattcaaac cacctgtaac aaaaagctgc ctttgacgag ctattgctat aaataagaga
     5281 tcttttttct ttaattattt atttatttta aaatggttaa gttttgctaa tcatgatgag
     5341 ttatttttga tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa atgacttaga
     5401 tttaaataag ataataattt tgttaacaat atgaggcggg agatgttttc tacaaactta
     5461 atacagagta attatggtga tttaaatatt aaaagcctgg ctttcgactc ttttaaagaa
     5521 aggcttgagt ctaccatggc ggcactcact ttctttatat caacgggtca atgtgactgc
     5581 gatgaaatag cagagtcgaa cttcaatcgt atgattgcct atatgtctaa cattaactat
     5641 gatgcctcaa agctaggagc gccagcactt agttttgata cgtatctgca ggacaatgtt
     5701 aaatatagag tgataattaa taatctttat ggcagtgagt ttcggataag agatattaat
     5761 gagaattttg ttgggatgga ttttacctgt gaattccgac ccgaaaaaat gacgaatttg
     5821 atcaatataa aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat
     5881 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
     5941 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
     6001 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa
     6061 acgtctcggg tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg
     6121 tattgataag ttggacattt ttttcgctga cg
//
LOCUS       DAJTRW010000170         6129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRW010000170 DAJTRW010000000
VERSION     DAJTRW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO326_003878"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO326_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5968069.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO326_003879"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO326_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5968070.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO326_003880"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO326_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO326_003881"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO326_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1614 a         1441 c         1449 g         1625 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacatt
//
LOCUS       DAJTRW010000171         6048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRW010000171 DAJTRW010000000
VERSION     DAJTRW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..143)
                     /locus_tag="OO326_003882"
     CDS             complement(<1..143)
                     /locus_tag="OO326_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5968071.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO326_003883"
     CDS             379..651
                     /locus_tag="OO326_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5968072.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO326_003884"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO326_003884"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5968073.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO326_003885"
     CDS             1111..1731
                     /locus_tag="OO326_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5968074.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO326_003886"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO326_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO326_003887"
     CDS             complement(3114..3767)
                     /locus_tag="OO326_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5968075.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO326_003888"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO326_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6048)
                     /locus_tag="OO326_003889"
                     /pseudo
     CDS             complement(5211..6048)
                     /locus_tag="OO326_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1426 a         1618 c         1598 g         1406 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattg
//
LOCUS       DAJTRW010000172         5991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRW010000172 DAJTRW010000000
VERSION     DAJTRW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            369..1277
                     /locus_tag="OO326_003890"
     CDS             369..1277
                     /locus_tag="OO326_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5968076.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1323..3314)
                     /gene="tkt"
                     /locus_tag="OO326_003891"
     CDS             complement(1323..3314)
                     /gene="tkt"
                     /locus_tag="OO326_003891"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5968077.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3592..4350
                     /gene="loiP"
                     /locus_tag="OO326_003892"
                     /gene_synonym="yggG"
     CDS             3592..4350
                     /gene="loiP"
                     /locus_tag="OO326_003892"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5968078.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4556..5476)
                     /gene="speB"
                     /locus_tag="OO326_003893"
     CDS             complement(4556..5476)
                     /gene="speB"
                     /locus_tag="OO326_003893"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5968079.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5612..>5991)
                     /locus_tag="OO326_003894"
     CDS             complement(5612..>5991)
                     /locus_tag="OO326_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968080.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1468 a         1566 c         1551 g         1406 t
ORIGIN      
        1 ttattttatt tttagggtac cgtccggcgg taaaaataaa atactcgtgc ggtttaatga
       61 aagcaattgg gtacgcgaga agaaaaaccc cacaaaccaa aataataaaa ccttaaataa
      121 caacaatatg aaattgatat ttttaaagtc aactttgtat ttgttatgtt catcacaaaa
      181 tcaacatttt gtttgttatt gtatttgtga tgcatatctc tatttattta ttgaatgttc
      241 gaaaacatac ttttctaatg caatgttttt tcgctatacc cctttataga ttctgaattc
      301 tcacctgtcg gctgttttct cctcatctca tgtgacatat tcccatactt tactcaagga
      361 agattgtcat gaaagcacct gtttcattaa cctcatttcg cccacaaaaa tctcttgcta
      421 tcgccattgg cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac accgttaatg
      481 aaggtgaacg cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc gaaccggggc
      541 tgggttttaa gatccctttt atggaaagcg tggagaaaat ttccacgcgt aatcaggctg
      601 tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa atgacagtat
      661 cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc tacaacacta
      721 ttgaagcatt aaaagatcgt ctaattgtcc gtcagttgcc aactcagctg gagaacattt
      781 tcggccaata taccgccatc agcgccgtac aggatcgtac aaaattggtt caggatctgc
      841 aaaatgccat gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc cagattgaaa
      901 atattgattt ttctgacgct tatgaaaagt cgattgaaaa ccgaatgaaa gcagaagtcg
      961 ccattgccac gcgtaagcaa aatctggaaa cagagaaaat ccaggcgcaa atagcggtaa
     1021 ctcaggccca ggcagaagca gacagtaaac tggcggcagc caaagcagaa gcggaaacta
     1081 ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg gctgaagcag
     1141 aagcaatccg cctgcgcggt gaagctctgc gggataatcc cggacttgta gccctcacca
     1201 ctgccgaacg ttgggacggt aaactgcccg ataccatgat ccccggcagc acagttccgt
     1261 ttatctctac taagtaaaaa aagggccgct ttcgcggtcc ttctcacaaa acgtcttact
     1321 gattacagca gcgcttttgc tttcgcaaca acgttatcaa cagtgaagcc gaactcttca
     1381 aacagcagct ctgccggagc agattcaccg aaggtggtca tgccgacgat agcgccgttc
     1441 aggccaacat acttgtacca gtagtcagca atacccgctt ctaccgcaac gcgtgcagta
     1501 accgctttcg gcagtacgga ttcacggtaa gccgcatcct gcttgtcaaa tgcgtcggta
     1561 gacggcatgg acaccacgcg cgctttcacg ccttcggcag tcagtttttc gtaggcagca
     1621 accgccagtt caacttcaga accggtggcg atgaagatca gttccggctg accagcacag
     1681 tctttcagca cataaccacc gcgcgcgatg tttgccagct gctcttcagt acgttcctgc
     1741 tgcgccaggt tctgacggga gaggataagc gcagtcgggc cgtcctgacg ctcaacgccg
     1801 tatttccacg cgaccgcgga ttcaacctgg tcacacggac gccatgtaga catgttcggg
     1861 gtcacgcgca gagaagcgac ctgctctacc ggctggtgag tcgggccatc ttcgcccaga
     1921 ccgatggagt cgtgggtgta aaccatcacc tggcgctgtt tcatcagcgc agccatacgt
     1981 acggcgttac gtgcgtattc cacgaacatc aggaaggtgg aggtgtacgg caggaagcca
     2041 ccgtgcaggg agataccgtt agcaatcgcg gtcataccga actcgcgaac accgtagtgg
     2101 atgtagttac ctgcagcatc ttcgttgatt gctttagaac cagaccacag ggtcaggtta
     2161 gacggtgcca ggtcagcaga gccgccgagg aattcaggca acagcgggcc gaacgcttcg
     2221 atagcattct gcgacgcttt acggctggcg attttcgccg gattagcctg cagtttagcg
     2281 ataaactctt tcgctttggc gtcgaagtca gacggcattt cgcctttcat acggcgggta
     2341 aattcagccg cttcctgcgg ataagctttc gcgtaagccg caaacttctc attccatgca
     2401 gattctttcg cctggcctgc ttctttcgca tcccactgag catagatttc agacgggatt
     2461 tcgaacggtg catatttcca gcccagctgt tcgcgggtca gggcaatttc agcgtcgccc
     2521 agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg gggaaccgaa accgatgatg
     2581 gttttgcaca tcagcaggga cggtttgtca gtcactgcgc gcgcttcttc aactgcgcgt
     2641 ttgatggatg ccgcgtcatg accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg
     2701 aaacgcattg ctgtgtcgtc ggtgaaccag ccttcaacgt gaccatcgat agaaataccg
     2761 ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg tacccgccag agagcaaact
     2821 tcgtgggaga tgccttccat catgcagccg tcgcccatga aggcgtaggt gtagtggtcg
     2881 acaatgtcgt ggcccggacg gttaaactgc gccgccagcg ttttttctgc aatcgccata
     2941 ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg tggtttccac accagcggta
     3001 taacctactt ccggatggcc cggagtttta gagtgcagct gacggaagtt tttcagttct
     3061 tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg
     3121 ccgttggaca gcacgaagcg gtcacggtca gcccaggacg gattctgcgg gttgtgtttc
     3181 aggaaatcac gccacaggac ttcggcaatg tcagccatac ccataggggc acccgggtga
     3241 ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct
     3301 ttacgtgagg acattttgac tccagatcgg atgatgaagg gcacgccctt aacgacttga
     3361 cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca atttaccgca agccgcgcgt
     3421 catgtacatg gaacatcctt ttgccgcttc agaaatctct ggatcatgct cgcatgttgc
     3481 gcaatctgct cgcccgtccg ctgcgctttt ccttatactg agactgagcg tcgattcacc
     3541 tgcaaacggc gcatttttag aataatcctg accttgtgcg gaagagaaaa catgaaaatt
     3601 cgcgccttat tggtagcaat gagcgtggca acggtactga ccggttgcca gaatatggac
     3661 tccaacggac tgctctcatc aggagcggaa gcttttcagg cttacagttt gagtgatgcg
     3721 caggtgaaaa ccctgagcga tcaggcatgt caggagatgg acagcaaggc gacgattgcg
     3781 ccagccaata gcgaatacgc taaacgtctg acaactattg ccaatgcgct aggcaacaat
     3841 atcaacggtc agccggtgaa ttacaaagtg tatatggcga aggatgtgaa cgcctttgca
     3901 atggctaacg gctgtatccg cgtctatagc gggctgatgg atatgatgac agataacgaa
     3961 gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt taggccatgt gaagaaaggg
     4021 atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg cggcctctgc gggcgggatt
     4081 gtcggcagtt tatctcaatc acaacttggt gatctgggcg agaaattagt caattcgcaa
     4141 ttctcccagc gccaggaagc agaagccgat gattattctt acgatcttct gcaccaacgc
     4201 ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac tggcgaagct ggaagaaggt
     4261 cgtcaaagct caatgtttga cgaccatcct gcatccgccg aacgtgccca gcatattcgc
     4321 gatcgcatca gcgcggatgg gattaagtaa agcctagtgg tgtcgaacgt aggtcagata
     4381 aggcgttcac gccgcatctg acattaatgg cacatttgtt accttgtgcg cattgccgga
     4441 tgcgatgctg gcgcatctta tccggcctac gggtgccgaa cgtaggtcgg ataaggcgtt
     4501 cacgccgcat ccgacattaa tggcacgttt tacccgtgcg catcgcatct ggtgcttact
     4561 cgcccttttt cgccgcctga atatacagca tctccagcgc cagcgtcgcc gcagccagag
     4621 cggtgatttc cgactgatcg tatgccggag ccacttccac tacgtccatc ccaacgatgt
     4681 tgagatcttt caggccgcgt accagtttaa tggcgcgatc ggaggtcagg ccgccaatca
     4741 ctggcgtacc ggttcccggc gcgaaagcag gatccaggca gtcgatatca aaagtcaggt
     4801 aaaccggcat atcacccaca atctgtttca cctgggcgat aacgtcatcc acgctgcgat
     4861 cgttcacctg gcaggcgtcc agcacggtaa agccgttgtc tttatcaaac tcggtacgaa
     4921 taccaatctg cacggaatga ttcgggtcga tcagaccttc tttcggcgcg gtatagaaca
     4981 tagtgccgtg gtcaaattca caaccgttcg cataggtatc ggtgtgggcg tcaaagtgta
     5041 ccagcgccat tttgccgaaa tgcttcgcat gagcacgcag cagcggcagc gtaacaaagt
     5101 ggtcaccacc gaaggagagc atacgcttac cggcagccag cagcttctcg gcgtgcgcct
     5161 gcagcttttc gctcatctca cgggcatcgc caaaggcata taccagatcg ccgcagtcca
     5221 cgacgttcag acgctcacgc atgtcgaaat tccacgggaa gcggttgtgt tcccaggcca
     5281 gattcgtcga aacctgacgg atcgctgccg gaccgtggcg accacccgca cgaccagaag
     5341 tggccatatc gaacggcacg ccagtaatca cccagtctgc atcgctgtca tacggctgga
     5401 agttcatcgg caggcgtaaa aaaccaaagg cattggaaac cagtgagtta tcgtattgat
     5461 gacctaaggt gctcatggat ggactcctat tacaaagtcg atataaaaaa acccttccgc
     5521 gtcgttaggc ccgacgagga agggttggat ttgcgaaaca caagtttgtg ctaattatcg
     5581 ccttaaatgg gcgaggcatc aagcgaggtt gctatttccg acacggtggc ggcgcatagc
     5641 cattgttatc tgccggaaag gcttgtccag agcacggcgg cagcgcgttt tgcgccgggc
     5701 cttgtaattc aaaggcggca aagccgatta ccccggtatt acgcacatcg ccttgcgccg
     5761 aattggctgc gtaggaatca gcaacgttgg cgaactgaaa agccgcttca gtatgtttat
     5821 ctttgcggaa tcctttaatc actaacgaat ctccggcatt cacaatatag ccgttgttat
     5881 tcagcgaacc ttgcttgccg ttcagcacat ccaggccatc agccgtagca acgatctcgt
     5941 agttggtgtt acgactgtaa ttacggacat ataattgata attcattccg a
//
LOCUS       DAJTRW010000173         5692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRW010000173 DAJTRW010000000
VERSION     DAJTRW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            92..502
                     /locus_tag="OO326_003895"
                     /pseudo
     CDS             92..502
                     /locus_tag="OO326_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            534..1148
                     /gene="istB"
                     /locus_tag="OO326_003896"
                     /pseudo
     CDS             534..1148
                     /gene="istB"
                     /locus_tag="OO326_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1317..5411)
                     /gene="sepA"
                     /locus_tag="OO326_003897"
     CDS             complement(1317..5411)
                     /gene="sepA"
                     /locus_tag="OO326_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5968081.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1406 a         1424 c         1354 g         1508 t
ORIGIN      
        1 gaaaaagctt attatgcttc catcggaaac gatgatctgg cagcctgagt tcacagataa
       61 aacactctcc aggaaacccg gggcggttca tgccgtcagg cagcagcgaa gcaaagcgtt
      121 actgacgtcg ctgaatgaat ggatggtgga gaagaatggt acgctgtcga aaaaatccag
      181 actgggcgaa gcgttcagct atgtactgaa tcagtgggat gccctctgtt attacagtga
      241 tgacggtctg gcggaggcgg acaataacac agcagaaaga gcgcttcgta ccgtctgtct
      301 cgggaaaaag aattacatgt tcttcggcag cgatcatgga ggcgatcgcg gagctctgct
      361 gtacggacta atcgggagct gtcggttgaa cggtatcgat ccagaagcgt atctgcgcca
      421 tatcctgagc gtactgccgg aatggccttc caaccgagtt gacgaactgc tgccatggaa
      481 cgtagttctc accgataaat aagcgtcaat acggccttcc gttgacgctt acagaactcc
      541 tgtggcgcga gagtgaaaaa ctgcgtcgtt ataaaaagga ggcccggttg ccagttgcca
      601 aaacgctaag cgaatacgac ttcattcagt tgccggaact gaatggagct cagttccagc
      661 aactctgtga aacgacagac tgggttgatg ctggagaaaa cgttctgctg ttcggggcca
      721 gcgggttggg gaaaagccat ctggcggcag cgatcgtgga tggcgtagta ggccagggct
      781 accgggcccg gttctacagc gcaggagagc tgttgcagga actacgtaaa gccagagctc
      841 agttgaaact gaatgagctg ctactgaaac tggatcgcta ccgggtgata gtggtggatg
      901 atcttggcta tgtcaaacgc gacagcgccg aaacgggtgt gctgttcgag ttaatagcgc
      961 atcgctatga acgtgggagc ctggtgataa ccagtaacca tccgttcagc atgtggggca
     1021 gcatcttcgt ggatgagact atggcggtgg cggcagcaga ccgactgatc catcacggat
     1081 atatgttcga actgaaaggg gaaagctaca ggaaaaagac agcgaaggca gtaacaagcg
     1141 tgacttgatg tcgcactgaa ggggcggcca gtatagttgg cgccagtcgg caaaactagt
     1201 tgacgtctaa tacctggacg gtcacttctg agaaaaggca tttacacaga atcttaaaca
     1261 ggctcacgga gttctttcag aaaaaggcct gccgaatggc agacctatcc cattcgtcag
     1321 aaaacataac ggaagttggc gtttatcgca ttatccacat tatatttccc gaacgctgat
     1381 ttctccagct ccagtcccaa acgcatatta tccttaattt ccgcattcat ccctaccgtc
     1441 atcagcatcc tgctatcttt ttcaccttcg aaacgtttct ctccggaagc atcctgcaga
     1501 accgtttctc cgttcgccag caggtcgaac tgataaccca gcccggctcg agctgtgatt
     1561 ttccagtcgt ctccggagaa ggctcttccc acgtcaacac cagtacggcc aatcagtggg
     1621 ttataatcct tgtctttcat gctcagagcc attccccggt cttcccagct aaaagctttt
     1681 cctgatacag aaccgtaaac cagctctatc tgtggctcca cccaggactc tttcgtcagg
     1741 tggtaacgat aaccaacttc agcaccggca taccaggaat gagagctgta gtcttttgtt
     1801 cccagtgagg caaaattcgc cgtgtgctga ttatcatgat ggagatattt accaatcagg
     1861 tcaaaataag ctccggaatt aaagagtgca gaggcataca gaccgccacc cacggatttg
     1921 ttttttccac tgaatgcgtg gctgcttgca ttgctgtccg tataggtcag caatgccccc
     1981 gtaaataaat ccacaccgtc cagctcatgc tttctgtcga caccaatctg aacgtgagtg
     2041 tagttatcgc tgtagtcacc atctgccgaa ccggtaccat tcattatgcg tgcccagaca
     2101 ccggcatccc cctgagtatc ccgcaggtca cccatacgtt tgttcaggtt attgacctct
     2161 gtaaggaagg acttataacc tgatgccata aagtcttttg cggctttcga ggctccggca
     2221 tcagcggtag tctgatatcc tgtcagcacc cattttgtgg catcatccgt tttttccgtg
     2281 ctgattactg gcgtaacgtt actgaaacca atctgctgcg taccggcagt gaacgttttc
     2341 tcatcactcc ctttcggtgc ggtaattaac gtcacgctca tttcactggc tggtttgttt
     2401 aaaaagtcaa ccaggacgct attattactg cctgacagtt tgtctgtgac attcaactgg
     2461 tcagcccggc tgttgttggt atgcatcagg aacgtggtat tatctgcaga aagctctttt
     2521 acttgtaaag tggcaaagtg tttcccgtcc cccagttcaa ttcgcgagcc cttagccttc
     2581 agtgcaccgg cctctgattt tccgtcagag taccacagag cattgatcat atttaccgta
     2641 ctctgtgccc ccgtaatact gcctctcaga acagcgttat atcccttcat taaggagctg
     2701 aataacggag agtccgtctt ccctgcatca gcatcactgt ctcccagatt aatggttgcc
     2761 gcagtggttc ctgcatgaat atccgaactc agatagccca tatttttaac agaaaagctg
     2821 gcgttatctt cgaggttaat cccttctgtt gtagaaatta cgggtgagta atacccctgt
     2881 ttctgcgcag aaggcatccc cgtcagggtc agagaagcat tttcttttac atccacagcc
     2941 ccggtgctga aaataccgct ctgagccgta agttttgctc cttttccgag ggtcaggctg
     3001 ctgttgacaa agctgccaac acggtcaaaa acagcattct gagaggtcac actcacggtg
     3061 ctatcataag cctcaatacc accggtgaat ctctccatga tttgcaggga tgatttattt
     3121 tccagcgtaa catttccgct gtaatcactc tggtcaccaa ctgtggttgc agtggcactc
     3181 ccttcaacag aggagaccgc attaccggtt ccgtcactcc tgtcagtaaa gacacggcta
     3241 cttcccagaa tgactgtact tccattggtg gcgttaatat ctccctgcat cttgccgttt
     3301 ctggacagat ggaatgttgc cgcatcgagt ttcagttttt ccagcgtaaa ctgacgggtt
     3361 tcccagtcgc tctggctgga agaagtcgtc gttccagcat gaataactgg atgaccctgg
     3421 aatatcagcg tgccggactt cttggttatc gaaccttctg gcaggttaac tgaaccatca
     3481 agagccagta catccttacc tgagagctgc ggaatattca catccatatt gccgtttagt
     3541 tggccgtgat acatataagg ctggctgctg gcctcaattt tttgttgctt cacagtatca
     3601 atggctttat tacgatcttt tccgacattc tgccagacac tgctgttgtt cagatcggag
     3661 aagaacgggg aatatgaact cgctttcagg atgaaatatt gcttggtaga actgtcataa
     3721 tataggtctc ccggcgctcc tctcccccca aaaatactga cagtattaac cggtacattg
     3781 atgtcactgg cttttaatgt ctgaaggtcg agtgttaccg ttgatttttt ctcactgtta
     3841 ttggtaatga tggccccgta gtcagccgcc tgaagacggg taaaggtcag attattacca
     3901 ttcagttcca gacgtcctcc acggtatccc cacgaaatat tatccggatt aacttgctgt
     3961 gagtcagaaa gcacaactgt cggacgaccg ctggcaatac caacagagct gaaagcctgt
     4021 acctttccgt cagcatccgc ctgctggttg agaataacgg taccgtcccc caccttgagt
     4081 ccgcctgcat tcacaccggt cccgtttact gtcagggtac cttcgccagt tttatgcagg
     4141 ttatcaccgg caactccgtt cacctgccac agaacgtggg ttcctttatc ggtgataatc
     4201 ccgcccccca tccatgtctg gccgttcagg gcggacaccc tgtaatcgcc ggcaaactgc
     4261 agatacccag ctccctgatt aacggaattc tgcagaacga cctcgccatt attgccggtg
     4321 aacaggaggt tttttcctgc gttcagatca tttcctttct ttccatgcat atcccaagtc
     4381 gtgtttccct gagtcagtgt gccggtgcca ttagccgcat catatttcca ctgcagtact
     4441 ccttcaccag aggtgtaggc tatagttttg tcaaaatcat tttgcggttg ctgaccaagg
     4501 aaatcctgag tggtgacgac ccagttattt ccatagttag ttcctgagga aagcacgcca
     4561 atcagtaccc attttttttg cagggaatca taagcaaaca ggggggagcc gctgtccccc
     4621 atattcagat aattagccag aggtccattc gcgggattaa aaatgtcacc ggtctgagct
     4681 gtaatcatct gtccgttgtt ataggagttc agcgcgctga cggttccccc aacaagaaat
     4741 ccgccattgg tatacacttg ggtgcgatta ccatttttgt ctttgacgta ctgcatcccc
     4801 cccccaaggc gatagaattc agtaaagcgt ccgccggcct gataggcacc gctcacagcc
     4861 ccgatgtcag acacttctgc cggcgccacc tctgtcacca gtttactcag gcggcgggtt
     4921 ttaatatcaa gaccgctatt attatttgta ccaaccgcag tataggtgtt tttggcgtat
     4981 ccgaagctca tcgtgtcaga accgctgaca tgtttggcgg taaccacata ctgaggggag
     5041 taaagtgtat ggtctcccgg aggcaggcta ccggtagtaa tagtggcact actgaaatca
     5101 gccatcgggg ctttatcaag tttaccgacc agattgccct gcttgtcgta aatggaaata
     5161 ttcgtggtcc ccggcgtaaa ctgaccttta ttctcggcaa agtcgcggaa tatctgataa
     5221 gggatctctg cactgaccgt tgctgacaga gctggccatg ccgaggaaag tgataaagct
     5281 gcaacagacg taagaataac ccgacgtgaa agtctgcgat ggcttttgca ggtaaccctg
     5341 cgggccagtt cggagacagc aatcaggctt ttggttatat ggcaatactt aagataatat
     5401 attttgttca tatagaaaca tcaacctaat tttgacaaag attaattaca taggatgtag
     5461 atggagcagt ccgagattct actaatatca attattaagc atctgttttt tagttggtgt
     5521 aatataattt ttattaaggt aagcggaaaa acaaccaaaa aaaacgcaag atgttatttt
     5581 tttatcattt tgagccagtt tggctggggg gatactcttc tcttaagctt cttgctcaac
     5641 tttaaaaata gttatgccag ttcggtatcg ataaggggca gtttggatat cg
//
LOCUS       DAJTRW010000174         5611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRW010000174 DAJTRW010000000
VERSION     DAJTRW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(111..1052)
                     /locus_tag="OO326_003898"
     CDS             complement(111..1052)
                     /locus_tag="OO326_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5968082.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1064..1553)
                     /locus_tag="OO326_003899"
                     /pseudo
     CDS             complement(1064..1553)
                     /locus_tag="OO326_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1557..2777)
                     /locus_tag="OO326_003900"
     CDS             complement(1557..2777)
                     /locus_tag="OO326_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5968083.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2781..3524)
                     /locus_tag="OO326_003901"
     CDS             complement(2781..3524)
                     /locus_tag="OO326_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5968084.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3415..4881)
                     /locus_tag="OO326_003902"
     CDS             complement(3415..4881)
                     /locus_tag="OO326_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5968085.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4881..5114)
                     /locus_tag="OO326_003903"
     CDS             complement(4881..5114)
                     /locus_tag="OO326_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5968086.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5102..5581)
                     /locus_tag="OO326_003904"
     CDS             complement(5102..5581)
                     /locus_tag="OO326_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968087.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1202 a         1579 c         1405 g         1425 t
ORIGIN      
        1 ccgagaaccg tcgggaagcg tgagcacaaa cggggtatgt acgtgtatta ctgcatcatt
       61 ttttgccatc gggtcatcct taatggcccc gccagggggc catgtggctg ttaaatgcca
      121 tcaacgtacg aaatggtttc tttgtacact ggctcgactg cacccagctt gccgtagtaa
      181 gtgacgatct gatacagacc gcgatactgc accggcacgc tctgaagcgg aaccagcggg
      241 tagcggacgt attttttatc gttggtgtac gcaactatgc gatccttatt ccccacacca
      301 cggcctttca gccatttaac cgcgcggata ttcagcggaa caccgttctg gtgatagctg
      361 atggtgttgg tctgaaggtt cgtcaacagg gactggttac ctgcagatga aacgatgatg
      421 ctggacaaca gagcaaactg ctcaggcggg atcagcaaat cacgcggaac cacagagtaa
      481 ccagaagcgg cccacgcatc agacagcacc tggttaatgc ttgcgcggat ttcgtccggt
      541 gttgaggttg cccacgtttt ggcagcgttg ttgacaggca cgccgtccag ggtaacaagg
      601 cctttcaggt ttaatgcgga atcgccaaca tatacctgtt catcgttatc catctgccat
      661 ttcagttgca taccgtcata cttctgcgta tcaatcgggc ggccgacctg ctgagcagcc
      721 tgcaattcta tgaccgtcca gccaagttcc atcccccaca ggttcagcgg gttaccggat
      781 ttgccgatat ccacgttcac gccagcaata gcggttgagt ctttgcctac ccagtttttt
      841 ccattcggat ttgcgccagt acccgcagcg gcgaagctgg tattcgtcca gctggaaatg
      901 tcatctgcga tggaggcgtc ttcacgcagt tgaatatcgc ggctccaggt gtaccccacc
      961 agtggcaggt tcagcgtctg gtcgagtctc tccagctccc cgatgagaaa ggcaccagag
     1021 ctgtcaacgg ttgcctgatc aaaagtaatc attcgtctgt tccttaaatc ttccaggaaa
     1081 tttctgcatt gccgtcagca tcaccggcac ctgtgaattc agcgttggtc agcaccacgt
     1141 ttttgccact gactgacgtg gacatgaatc cacccagagg cactttgatg gattcatcag
     1201 tggagatcgc cttttttgat agtgctggca tcaaaatcag aaccgagatt aacggtcacg
     1261 tagccacgct tcatggcgtc gcccgggaag ttcttgcctg ttcccacctg gcgaaccatg
     1321 tccggctgcg acgtggtcgg ataagggcgc acgtagatcc ccttcacctt gtctgcggta
     1381 tcaccatctg ccagcggcac gaaaaaaccg tcatcatcgt atttaccagc caggccatag
     1441 gcagcgaagg cgttatcgga tttaaggacc accggttcga cggttaagtc ctgcgggcga
     1501 gagacagccc cggcaatgcc aacaggcatc cggtacagaa atacattatt cattttttac
     1561 cctttacggt ttgcccagaa ttcagcgttt tgtttgttca gggaagcgat actggtcatg
     1621 cccatgttta ggcgctgtgc atcgccggtg gtggcgcggg tgtttcgccc tttggcaacc
     1681 tcagacacgg cattaaacgc catgtcgacc gattgtttcg gtaatttgcg gatatccgca
     1741 tcaccgacta tctggcgaac cagcgttttg tcagcagaag ccagaacctc gcgtttgaac
     1801 gcggtcggtt tcatcttacg gctcagatcg atacccggaa cgataacttc ggcacgccag
     1861 gctgagtcac cagtaatcgt ggtttcctct tcatcgtcct cgccgtcacc ggtcggatta
     1921 tcgtcaggct tattatcgtt atcgcccgtg gcatttcttt ccagcttagc cagcagggct
     1981 ttcagtaatg ttttgaggtc atcatcactg tcgccggttg gatctccgcc catctctggt
     2041 gctttgtccg gtagtggttg ctgcggggac aggttgatgt tgagattaac gccctgcggc
     2101 aaatccccct catctcctgt aaccgatgcg ggagccgact ccaccagttc gttcatggtg
     2161 tcggcatctc ctgtcttgat ggccgcacgc atgcggttcc accagttttt cttttgattt
     2221 gccattgtgt ctctgtctcc aattgcacaa cgatttccgg ctctgccttt agggacaaga
     2281 gccacatggt ttccggtaat atcgacctgc tcggctttac ctggctcggt ctgctcgtac
     2341 tccgcgtcat agccgcacga cacttcgcgc aggccatctt cgataagctg aatggcgctt
     2401 tcgtctttga cgataaggtc agccagcatc aaatcagact gctcacccgt cccgcgccgg
     2461 acattctgga ggtgcccgac agcaagcact ttccagttct cgggatttac cagccgcaca
     2521 ttcccgtttt catcttcagg atgcaggatc gtgatgctca tcccttcgaa tgaggcaagc
     2581 gtggccggat ggaatacctg ctcaggagaa cgcgtgacga ctatttcaac gaacttatcg
     2641 ggtttcagtt ttggcaggtc atcagcacca tagagctgct tacctgttcg tcctatcggc
     2701 acgtctttgc acagcaacga gccgtcagcc aggcggtatc gggtttcccc gagcctggtc
     2761 tcaaaaaagt atttcatggg ttatgctgcc cttttcattg cgaatggaac gcgaacaaac
     2821 acgatctctt tatagcagcg gcagtttggc agctcgcccg cgtggccggt catgccgtcc
     2881 aaagttggag gtgaatccca gcggacgaat ttacctttca tgtgatcgtg ggaatctcgg
     2941 acatcgccat catcggctgt acgccaaata taaccatccg agccaatagc tatcgctcgg
     3001 gccatatcca gcgcgcccgt tgctcttccc agttccgttc gggcaatcag atcggcccta
     3061 gacttagcaa catcgccggt agatgcaatc tctttagcaa actgctccgc ccgaccaccg
     3121 gtgacaacag cctctatcgc tttgttctgg atgtcgtaca ccctgtctgc ggcctcgaga
     3181 ggcagagatt tgatgtactt gacctgttcg gcgatgatgg attgcatcac ctggcccaca
     3241 ggagcgcttt ccacaagatt gcggagctcg cgactgatgt tcttgctgtg ttgccgccaa
     3301 actttctcgt tctgccgggt taggtccgca gtaaagtttt ccgcgacctt tgtcgcccag
     3361 ggggtgatga tttcactgta acgctccagc gccgcaataa tttccgtgat actgtcattt
     3421 gaaccatcgt agcgaccatt tacgatgtct ccgaccgccc tcgctatcct gcgtaggctg
     3481 gttcgatacc ggatttccgc ctgacggctc ctgcggttcg tcatcaggtt cgccgatgcc
     3541 gggcggcgct tcatcttcgg cattctcgat gtcctcgttg gtaatggatg cccctatgcc
     3601 ggttacgtca gaattttctc gcaaatcagt catagcggct ttcagtgtca tcagaccatc
     3661 acccagcgcc gtactgattg cgttggtggt gtttaacgcc accgttgagc gatcgacatc
     3721 agacatttgc cagagcgggt taaactcaaa cgtgaaatcg tccggcagcg gcttgccaag
     3781 ttccgaacga tgcatgatgt ccagtatccg ccgcaccgga agacgtaaac gcctctcctg
     3841 caacgaactg atgcgatcgt aatagttggc aaggtctgca tcaccggtag aaaatccttt
     3901 cggggactgt ccgaacaacc gcaccagtgg gataccaaca gcgccactaa tctgttctgc
     3961 aaactgcgaa aggatgtcat ccagaccact gaagctgtac tgatgggttt caaacttatc
     4021 ccgcgagtcc atgagcgtca tgccttcatt actctggaac tgtcgaatca ggtcgatatt
     4081 cttcagcaac gcttcatacg caggaccacc aagtgcgata agctcgcgta gcttctccac
     4141 gctgtaggta cgcaaatgcg ccttgtagac cagctgcgcc gcgccgacag tagcgctgtc
     4201 gaacgcggta agacgatccc agatacgctc tacaaccgac attccccatt cgttctcggt
     4261 catcttctgc tgaaatggca acgtgacgcc atcaaagcga atcaggcgac tgtgatgaat
     4321 gcgccaggca ggaattcccg ttgctgtggt caccacatcg taaaactcag gtttacccag
     4381 gtccggcccc atatctttaa tgcggcgggt cagtgccggg tcgaccatcc agcggtcgag
     4441 cgggagaatc cccttaaact tgcccttacc gatggtttcg ggtcgcagcg gggtcattgg
     4501 tgcctgcccc tcaatcatga tgaaacccac cgcgccgccg tagaggcgcg accatttcag
     4561 cacgtcattc agcgcatccc agatttgcaa ctcatccagt tgtgattcga gaatgccacg
     4621 atcttttgca tcaatttccg aagtgatgcg aatgcctttg cgggtcatat catccgggat
     4681 agcatcgact gcttcgccga tgatccagga tgaacgatag gaccattcca ccagcatgcg
     4741 gttacgactg gtgaaattag cccggtaggt ggatgctgag tgctggttag gtgtctgcat
     4801 ccctactcgg gcaataaaat tctcataacc atcagctgtg gcctgcacag ttcgccgcag
     4861 ggcttgtttg tttcgtgcca tcaggcctgt ctccccagct gttcccagat atccagcgat
     4921 gtatcgattg gcgcgaaggc cataatgaat gcatcagcaa cgtttggtga cggtatctcg
     4981 cgttttgcga ggtctttttt actttcgacc atcacacgtc cgttacggtc gaaatcacga
     5041 tgaggtgttg tcagttccag tttcagcttt tcaagcaacg gacaacgaga atctatgctg
     5101 atcagctcat ccacaggata ctgttctccg ttgttaatgg cgttaaacgt atttctgaaa
     5161 cggtcagcca ccatgcctga gccttaagat ttgcgaaaaa gtctttgttg gggatgccgt
     5221 tgtactcgtc atctggctca tgcacaccag caccggcgtt aaacctctgg taattcacac
     5281 gtcgcgcgta tgcgttctcg ctcttccggt cagcgttaat ttcagagaat ttagcaccgg
     5341 cagacgcacc aacaccgata gagtcgtaaa caatatctgc ttcacgctcc agcgccgcct
     5401 gataagtacg ctggcagctc ttcagtaatt catcttcttt ggccttccat tcgtcggccc
     5461 agaaaacaac ggatccgtga cggtaaacgt tagcgcactt atctgtacca ctgtcagcca
     5521 cgtcaaagcc aatacgcttt cttccactgg gttcgaaatt taacgttttg tgcgcatcca
     5581 ctgcggcttc tatccaagac agtttgatga t
//
LOCUS       DAJTRW010000175         5558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRW010000175 DAJTRW010000000
VERSION     DAJTRW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..641
                     /locus_tag="OO326_003905"
     CDS             <1..641
                     /locus_tag="OO326_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5968088.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO326_003906"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO326_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5968089.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO326_003907"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO326_003907"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5968090.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1378 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaa
//
LOCUS       DAJTRW010000176         5472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRW010000176 DAJTRW010000000
VERSION     DAJTRW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..279)
                     /locus_tag="OO326_003908"
     CDS             complement(1..279)
                     /locus_tag="OO326_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968091.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO326_003909"
     CDS             220..405
                     /locus_tag="OO326_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968092.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO326_003910"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO326_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5968093.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO326_003911"
     CDS             complement(1254..1670)
                     /locus_tag="OO326_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968094.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO326_003912"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO326_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO326_003913"
     CDS             1837..2862
                     /locus_tag="OO326_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5968095.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO326_003914"
     tRNA            complement(2915..3004)
                     /locus_tag="OO326_003914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO326_003915"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO326_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5968096.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO326_003916"
     tRNA            3996..4071
                     /locus_tag="OO326_003916"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5472
                     /locus_tag="OO326_003917"
                     /pseudo
     CDS             4233..5472
                     /locus_tag="OO326_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1465 a         1297 c         1279 g         1431 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttagacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tg
//
LOCUS       DAJTRW010000177         5467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRW010000177 DAJTRW010000000
VERSION     DAJTRW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..1620)
                     /locus_tag="OO326_003918"
     CDS             complement(<1..1620)
                     /locus_tag="OO326_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5968097.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWYPQLELIAGVLTSHKDG"
     gene            complement(1636..2520)
                     /locus_tag="OO326_003919"
     CDS             complement(1636..2520)
                     /locus_tag="OO326_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5968098.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2531..3136)
                     /locus_tag="OO326_003920"
     CDS             complement(2531..3136)
                     /locus_tag="OO326_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5968099.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3307..>5467)
                     /locus_tag="OO326_003921"
     CDS             complement(3307..>5467)
                     /locus_tag="OO326_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5968100.1"
                     /translation="VEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLG
                     NLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTI
                     KGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLIN
                     DEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDI
                     ATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQP
                     VVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELY
                     KDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEM
                     KHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAY
                     FCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPK
                     ADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVL
                     KELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWW
                     STLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPL
                     LALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1360 c         1136 g         1390 t
ORIGIN      
        1 accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt accacggctg
       61 cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg cgagtgagtt
      121 ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg ccagcgtagg
      181 ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt caaaacgcac
      241 ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat ttaactcact
      301 gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat ccagtacata
      361 gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg cgtggagatc
      421 ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa taccgcgcca
      481 tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac tgccttgcgg
      541 ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac tcattccgag
      601 atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa agcgatacgc
      661 taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc gatccgctga
      721 tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag catataagcg
      781 gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac ccaatgtgtc
      841 gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc tctgataaaa
      901 atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa gttgcgtggg
      961 gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga gatcgagcaa
     1021 gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg ccacccgttg
     1081 ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata gaccacgcag
     1141 acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa ccttaaaggc
     1201 cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc atgtggcaac
     1261 aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga cctggctgtc
     1321 gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa gagacgggct
     1381 ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat aaaaagcagt
     1441 atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt catgcccgcg
     1501 atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt gccgataata
     1561 caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg aattttccat
     1621 tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata ccggagcggt
     1681 gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata gttttccact
     1741 atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc aaaaaccact
     1801 aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg tcagcaacgg
     1861 tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac cttccagcgt
     1921 ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc cccataaata
     1981 ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta acgccccgcc
     2041 ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg caaaaacggt
     2101 cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg ggtcacccac
     2161 aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata acagaaacag
     2221 ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat ataaccaacg
     2281 cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac ttatcagcgt
     2341 aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac aaatgacgat
     2401 gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag gtaaccgcca
     2461 tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt tgccagagat
     2521 ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga ttaatcgcag
     2581 tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc atccactgtg
     2641 gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca cttttgccaa
     2701 atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg caaaactcgg
     2761 tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc ggtaaaaata
     2821 aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc aaaatcgccc
     2881 ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca ttgagcgcca
     2941 tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc accaacacca
     3001 acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga ttcgctgtaa
     3061 cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag agcggtttta
     3121 tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt gcatcaattc
     3181 ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata tcaaggtatt
     3241 gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa attattacgc
     3301 tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg cgcgcgtctt
     3361 accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct ctaacgccag
     3421 caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg gattattcat
     3481 cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg gggtgtggcg
     3541 gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg tactccacca
     3601 tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg ggtcgagtgc
     3661 ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct gcctcagaaa
     3721 aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga gaacaacagc
     3781 gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt ccaaacgtat
     3841 cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa tcagctcaga
     3901 caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat tatttttccg
     3961 atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat actgattttt
     4021 cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt gtaaagcagc
     4081 gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt ttttctccat
     4141 ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca cacgtattag
     4201 atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac tctcttgtgg
     4261 cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg ctaagcttgt
     4321 gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag tgagttgtcc
     4381 tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat caataatgga
     4441 ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc aatctctttt
     4501 cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat tcaacggacc
     4561 gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg gttcacggtc
     4621 gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact ccagaatctc
     4681 attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc tattaataag
     4741 ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca ttatggcaag
     4801 atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat ccagcatttg
     4861 caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt ttttcatctg
     4921 attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt tcagagcatc
     4981 ttcatcattt attagacccc aataatatgc atcaggattt attatcatca gcgctttttt
     5041 gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat ttgtcgaaga
     5101 cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa tactcccctt
     5161 aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg catttatcga
     5221 accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt taccgagaat
     5281 aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca aattgccaag
     5341 aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc cacgatgcac
     5401 tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt tgatagcttg
     5461 ttcaact
//
LOCUS       DAJTRW010000178         5388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRW010000178 DAJTRW010000000
VERSION     DAJTRW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..487
                     /locus_tag="OO326_003922"
     CDS             <1..487
                     /locus_tag="OO326_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5968101.1"
                     /translation="FPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIAN
                     EPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAA
                     PSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESA
                     H"
     gene            509..2500
                     /gene="cyoB"
                     /locus_tag="OO326_003923"
                     /pseudo
     CDS             509..2500
                     /gene="cyoB"
                     /locus_tag="OO326_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2490..3104
                     /locus_tag="OO326_003924"
     CDS             2490..3104
                     /locus_tag="OO326_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5968102.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3104..3433
                     /locus_tag="OO326_003925"
     CDS             3104..3433
                     /locus_tag="OO326_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5968103.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3445..4335
                     /gene="cyoE"
                     /locus_tag="OO326_003926"
     CDS             3445..4335
                     /gene="cyoE"
                     /locus_tag="OO326_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5968104.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4519..4830
                     /locus_tag="OO326_003927"
     CDS             4519..4830
                     /locus_tag="OO326_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5968105.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4965..5363
                     /locus_tag="OO326_003928"
     CDS             4965..5363
                     /locus_tag="OO326_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5968106.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1173 a         1341 c         1423 g         1451 t
ORIGIN      
        1 tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt
       61 cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca
      121 tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg
      181 cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg
      241 ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact
      301 ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt
      361 tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga
      421 aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc
      481 ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc
      541 gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct
      601 cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc
      661 cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg
      721 tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggtgaagc
      781 gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat
      841 cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat
      901 cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt
      961 tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct
     1021 ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg attactggat
     1081 atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac
     1141 cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat ttacctaggc
     1201 atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc
     1261 gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa
     1321 catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat
     1381 cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt
     1441 tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt tcatcgtttg
     1501 gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac
     1561 cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta
     1621 tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta tcgtcacctt
     1681 ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca
     1741 taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg
     1801 ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac
     1861 ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct
     1921 gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt
     1981 ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg gtattctctg
     2041 cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg
     2101 cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa
     2161 ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga aagagaaagg
     2221 cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc
     2281 aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg
     2341 gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga
     2401 cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt
     2461 cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg
     2521 cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga
     2581 tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg
     2641 tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg
     2701 ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt
     2761 acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg
     2821 gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc atggggccgg
     2881 atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca
     2941 cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca
     3001 gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt
     3061 ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga
     3121 tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat
     3181 cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat
     3241 tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct
     3301 gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct
     3361 aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca actacaacat
     3421 gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca
     3481 ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc
     3541 agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg
     3601 ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag
     3661 aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg
     3721 ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg
     3781 ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa acgccactca
     3841 gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat tggctactgt
     3901 gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg
     3961 cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac
     4021 attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat
     4081 atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa
     4141 tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat
     4201 aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc
     4261 actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca tacgctgctg
     4321 gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct ttttattgtc
     4381 tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga tgcacataaa
     4441 ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag
     4501 ttaatcaaag aagtcataat gcttggaaaa acagccgctc tatacgatgt cgataaaacg
     4561 ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa
     4621 gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc
     4681 gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg
     4741 atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa
     4801 aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg
     4861 taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt
     4921 tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt aatagcgact
     4981 gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc tgtactttta
     5041 cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca caaccttcag
     5101 aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa aagcggggct
     5161 ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac ccggcattac
     5221 caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat gctcgtctcg
     5281 gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg tttttggcga
     5341 ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTRW010000179         5366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRW010000179 DAJTRW010000000
VERSION     DAJTRW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..170)
                     /locus_tag="OO326_003929"
     CDS             complement(<1..170)
                     /locus_tag="OO326_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968107.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO326_003930"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO326_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5968108.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO326_003931"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO326_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO326_003932"
     CDS             complement(3616..4383)
                     /locus_tag="OO326_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5968109.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5366
                     /locus_tag="OO326_003933"
                     /pseudo
     CDS             4579..5366
                     /locus_tag="OO326_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1320 a         1387 c         1391 g         1268 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgcc
//
LOCUS       DAJTRW010000180         5340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRW010000180 DAJTRW010000000
VERSION     DAJTRW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO326_003934"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO326_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5968110.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO326_003935"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO326_003935"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5968111.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO326_003936"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO326_003936"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5968112.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO326_003937"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO326_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5968113.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAJTRW010000181         5172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRW010000181 DAJTRW010000000
VERSION     DAJTRW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..736
                     /locus_tag="OO326_003938"
     CDS             <1..736
                     /locus_tag="OO326_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5968114.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OO326_003939"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OO326_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5968115.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3597..3800
                     /gene="safA"
                     /locus_tag="OO326_003940"
     CDS             3597..3800
                     /gene="safA"
                     /locus_tag="OO326_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5968116.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3868..4629
                     /gene="ydeO"
                     /locus_tag="OO326_003941"
     CDS             3868..4629
                     /gene="ydeO"
                     /locus_tag="OO326_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5968117.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5031..>5172
                     /locus_tag="OO326_003942"
     CDS             5031..>5172
                     /locus_tag="OO326_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5968118.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1479 a         1101 c         1154 g         1438 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc
     3601 atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg
     3661 taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc
     3721 agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     3781 gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat
     3841 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     3901 catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg
     3961 atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc
     4021 ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     4081 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     4141 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     4201 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     4261 gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     4321 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     4381 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     4441 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     4501 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     4561 aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta
     4621 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     4681 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     4741 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt
     4801 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
     4861 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     4921 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     4981 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     5041 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     5101 catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt
     5161 tctcagactt ta
//
LOCUS       DAJTRW010000182         5143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRW010000182 DAJTRW010000000
VERSION     DAJTRW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..222
                     /locus_tag="OO326_003943"
     CDS             <1..222
                     /locus_tag="OO326_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968119.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="OO326_003944"
     CDS             complement(394..723)
                     /locus_tag="OO326_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5968120.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="OO326_003945"
     CDS             complement(728..913)
                     /locus_tag="OO326_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5968121.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="OO326_003946"
     CDS             complement(910..3549)
                     /locus_tag="OO326_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5968122.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="OO326_003947"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="OO326_003947"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5968123.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5143
                     /gene="hslJ"
                     /locus_tag="OO326_003948"
     CDS             4857..>5143
                     /gene="hslJ"
                     /locus_tag="OO326_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5968124.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANP"
BASE COUNT         1300 a         1318 c         1200 g         1325 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattagccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaaccagca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa ccc
//
LOCUS       DAJTRW010000183         5142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRW010000183 DAJTRW010000000
VERSION     DAJTRW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            38..778
                     /locus_tag="OO326_003949"
     CDS             38..778
                     /locus_tag="OO326_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5968125.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="OO326_003950"
     CDS             837..1223
                     /locus_tag="OO326_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5968126.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="OO326_003951"
     CDS             1232..1561
                     /locus_tag="OO326_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5968127.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="OO326_003952"
     CDS             1533..4574
                     /locus_tag="OO326_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5968128.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="OO326_003953"
     CDS             4574..4903
                     /locus_tag="OO326_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5968129.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..>5142
                     /locus_tag="OO326_003954"
     CDS             4903..>5142
                     /locus_tag="OO326_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5968130.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLT"
BASE COUNT         1263 a         1167 c         1676 g         1036 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cg
//
LOCUS       DAJTRW010000184         5098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRW010000184 DAJTRW010000000
VERSION     DAJTRW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..304)
                     /locus_tag="OO326_003955"
     CDS             complement(<1..304)
                     /locus_tag="OO326_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968131.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYK"
     gene            complement(372..2321)
                     /locus_tag="OO326_003956"
     CDS             complement(372..2321)
                     /locus_tag="OO326_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5968132.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2406..2741)
                     /locus_tag="OO326_003957"
     CDS             complement(2406..2741)
                     /locus_tag="OO326_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5968133.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2741..3097)
                     /locus_tag="OO326_003958"
     CDS             complement(2741..3097)
                     /locus_tag="OO326_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5968134.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3097..4593)
                     /locus_tag="OO326_003959"
     CDS             complement(3097..4593)
                     /locus_tag="OO326_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5968135.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4590..4751)
                     /locus_tag="OO326_003960"
     CDS             complement(4590..4751)
                     /locus_tag="OO326_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5968136.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4761..>5098)
                     /locus_tag="OO326_003961"
     CDS             complement(4761..>5098)
                     /locus_tag="OO326_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968137.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1190 a         1503 c         1118 g         1287 t
ORIGIN      
        1 ctttatatat tgggatcaaa aatgtcatcg tcgctggtga tgcattgttt gcaaaatcaa
       61 atacacctat acttttgccg aattcataag aagcttttct caatataatc gcaccgctgg
      121 cagaagggga tgggcacgat atcctcagtt gtctgaatcc atctgtatcc aagtcaacgt
      181 cattatctgc agatgtttta acgttactaa taaatcgtga aacaatctcc tcatttgtta
      241 gtattttcgc ctgagcacag caagaggtta atattgcaac cgtcattaaa aatggcttta
      301 tcacaaaaca aactccattg ttagcatgtt acgcgataat tttgcctcat ctttcaccac
      361 aaaaaaagaa ctcacctttt aaatggagaa tacccaacat catgggtgat tttcataaag
      421 ggatcgcctg ttttcggcaa atcgataacg cgcattccag gcggcgcgtt gtcaaacgtc
      481 accttaagtt cgctgcgggt tgccggtgaa gatgcaaggc ttaacagctg attcctttcc
      541 agtgatacat tcggggtgta gcgtttatcg ggagataaat acccatcacc acgtggcatc
      601 tgccatccgg tcatgtcata gacaaaatca tggaactgtt tgccccactc gtcgaactta
      661 tcattcagac caaatccact gttcagtgca tcagccacaa aatttttaac aagccaggga
      721 tgttcttttt caaactcctg cgccatcatg cccagttcaa gcaatccacc aatcagactg
      781 atctttccga ggccctttaa tttcagccct gtttttcctg ccgccgttgt ccattgattc
      841 tgggcgactg ttgccgcacg aatacttgcc accgccttca caccaatata cagggcagaa
      901 accgttgcta ttgtttttac tgcctgttcc catcccccca ttgctctgac aacctgatca
      961 acctcctgcc agactttttt cacaaccgga ccgaccgttt cccagttatc aattatgaga
     1021 tatgcaccac cgacgagaag cgcaattaat ccctttgccg gcgtcatatt catcacgcta
     1081 cccatgattt ttgttatgcg tgtcaatgtg ccaatcgcca cgcccatcgt cagtaatgcc
     1141 gcaccagttt tcgcgatggt ttttacgacc tcaggatttt ctctgacaaa cgtgcgcact
     1201 tcttccagaa aaggcttcat ctcttttatg ccttcattga gtgaaggcag gaaggtttcc
     1261 cccagcgtgg aagaaatcgc attgatctgg ttctgcagca gcaacaactg attttctgtc
     1321 gtcgcggcac gggcggcata ttctttctgc attgagccgc catactgctg cgcatccgcc
     1381 acacgattaa agttggttcg caacaaatcc agattcgtga gcagcggcgc aatggccccc
     1441 agggattcct tcccgaacag cgctttcagc acagcagcct gtttttcttt cggcactttt
     1501 gccagagaat ccagtacgtg cagcatggcc ccgcgggcgt ctttctgcat atccgccgcc
     1561 agttttttcg ggctaatgcg caacgagcgg agcacttttt tctgcgattt ggtggcagaa
     1621 tccctcgctg tcagcgacaa cataaaattc tttatccccg tcgcagcaat ttctgactcc
     1681 acccccattc cggcaatggt tgcccccatt gcggcaatct ctccggaggc cacacccgca
     1741 acactgccta aagggccaat acgggtcaca acatcagaaa tctttttcgc acttgcagga
     1801 ccggtattgc caagatagtt aatcttatcc gcaagccctg ccacctctcc ctgtgtcagt
     1861 ttaaaggcag tgcgccactg cgccatcatc tgaccggatt cttctgccgt ggtgtcaaag
     1921 gccacgccca tcttcacggc atcgtcagta aactgcatca gctcgtcacg ggcgatacca
     1981 gcctgaccac ccgccgccac aatttcggcg ataccttccg ccgacatggg cagttctgtt
     2041 gacaggtcgc gcacctgctc cgtcattgcc ttaaacgctt ccggcgtatc cagaccatcc
     2101 accactttcc ggacatccgc catttttgat tcaagggcaa tggcagattt gaccgggagc
     2161 gccagcgccc ccagtactgc ggttccggca cccgctgcgc ccagtgacag gctggcaaac
     2221 tcctttttaa aacctttcag ctggcgctgc attcccttca gcggtgccga taccttatcc
     2281 acggcagtga tgatcgcctt cagctgaaaa ctgtcagcca tgcttcatct cctcgttaat
     2341 gcggacggcc tcggcttcca gttctgtaaa ctgcgaaaca gccacccgtc gcagctccag
     2401 tggattcagt ttccagaacc aggcaacatt gtagagtcgt ttccggaggt gcttcccgtc
     2461 tccgactggg taaaaaaacg caggatctgc atgctggtct taaaaatatc cagttttgcc
     2521 atctgcgccg ccgatgaaca cgggatcccc gccagcaacg ggatatattt cagtgccacc
     2581 tggctgtcca gtttaatact gccttcaccg gaaataatga aaggaaaacc cagcgcctcg
     2641 atttcgtcat acgtgggctc gcgcaactcc agcacatgca gcatttcgtt atgcgctgtc
     2701 accggttttt ttaaaactat ttctgtaacg ttcattgcca ttactgatac cctccctctt
     2761 cgccgtggaa ttcaagatct gccgtgcctt cttcggcatt atgattagcc tctccgtgca
     2821 gccatgccgc cgaaagcaca tacaccatgc cgtttgccag ctcagcggta atcgtcatct
     2881 ggtctgaggt ggtaattttg tcgaccggaa agtttttggg cactttaaac gtgcccttaa
     2941 cgtaaggtga ccgccaggtc tccttgtaat ccacatcccc tgccatgccg acgatgtcat
     3001 cccggacatt ggtgttcatc ggcacttcaa tgccacctgt cagcgataac tgctgaccat
     3061 ccactttaaa aaaacaggtt ccggcgatct ttgccattat gctgactcct ctgaatactg
     3121 aagacggaac tggttaacca ccgcaaagac acgtaactga ttaacataat ccggcgggaa
     3181 cagcgtattc agtcggttcg ggttatctgc atcacgctca actatcagat actgtttaaa
     3241 cagatcgtat ttttccacaa taccggcacg ctccatctga cgatatgtcg ccagaagttc
     3301 ccctttgata acggcaggag tgacaatcgc ctgccccgga ccaaaacggg taccatcatt
     3361 tgccagcttg tgacgtccgt acttgcttgt gatgaccgat ttcagtttgc gaagaacata
     3421 tgcactggta tgcagagttt cactgtccag atagctgttg tctgccacgc catacgcatt
     3481 ctttttgtag gtggttacgg aacgctggat ctgcaacgtg ccgccttcca cataagccgt
     3541 cgccacgccg tgagataaaa gggtctgctg ctctgtcatg atgaatcgct tacctttcgg
     3601 tgccggaagc atccccacca gctcccccgt ctgtgtcgga cgggcaggat cattccggat
     3661 aaataccgct tcacgggcaa ggcgactggc aaccagttca tcgacaggcg actgggtttc
     3721 tttttcgtaa ccggcaagcg tgatatgttg ctgattatgc atatctccgg caccaaccag
     3781 ctctgacagc gttcccagtt ttgcggtata gacatgcccg tataactggc gcgcataact
     3841 ccagcgaccg ctgctgtcat tcatttcggt catcatcata ttgatggagg cggcatcgtt
     3901 gaacggcaga ccgataaagt cgaatgcctc atcgcccata gcagcaacag cggcggtaag
     3961 atcaggcgct ccgctaccgg cagcaccgac ttccgtcacc acccgaagcc ctgctggcaa
     4021 aatctcacca ccaccagaac cataataatt cagacagacc ggtaactcat taccatacag
     4081 ccccttatga cgggcagtca gcgtcaccac gcctgcatca gatgacgccg taaacggcag
     4141 ggtgataacc ccgtttaccg cttccttaat cgcggtggca accgcagtgg catcatcgcc
     4201 attcaccaca ggcacctgta cacgggagcg cccgacatac agactcaggg tgccgctttc
     4261 ctctgcttct ccggcaaccg tcaccctgac cgtcgccgcc gcccctctgg cttccggtac
     4321 tgcaataaca tacagttcac cgaaaggatc tgtctgacgg tagacttcca ccatacgcgc
     4381 cagctggctc cccgccccac aaatctggcg ggcataatct gccgatggca tcagcaccag
     4441 gctgttaacc tcaatggctg catcgttgct ggcatgcccg atcagtaatg caggcgcgct
     4501 ggtcaccgct gtatttgccg cagaattgtt catctcggcg tagaacaacg gcaccagcgt
     4561 attcgacgga acagtattaa aacttatcgt catgattttt tagccttatc ctgtttaaca
     4621 cgtaccacat cacccgccgc tatacggcgg aaccagtagc tgctttcttc cacatttcgc
     4681 ccttcggaag gcaaaaggtc tccacgggca gggtcaggaa ccgaccgccc tttcaggggt
     4741 ttcacaaaca tgagggactc ttaattctga gggaaacgca tttccagatg gtgctcaatg
     4801 tcaccatccg ggccagtacc aggatcgatg taatccacat caatactcag cagggataaa
     4861 tccggcaggg cattcacgtc ctctgcctgt cgcgtgtctt cttccgtgat ttcatacttc
     4921 gccgtaaaat caaactggta atacagttcg taacggttca gatccagcag ggtgccaccg
     4981 acataaacaa tctcgccccc ttgcgggtcc ggcatccatc caagcagggc tttccagagt
     5041 tctctccgga catcatgaac ggcgtcatag gctgcccact gccctttttc atcacgct
//
LOCUS       DAJTRW010000185         5080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRW010000185 DAJTRW010000000
VERSION     DAJTRW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..935
                     /gene="tauA"
                     /locus_tag="OO326_003962"
     CDS             <1..935
                     /gene="tauA"
                     /locus_tag="OO326_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5968138.1"
                     /translation="AALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVD
                     WRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKK
                     TISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDI
                     DGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSA
                     IDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPV
                     NKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            948..1715
                     /gene="tauB"
                     /locus_tag="OO326_003963"
     CDS             948..1715
                     /gene="tauB"
                     /locus_tag="OO326_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5968139.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1712..2539
                     /gene="tauC"
                     /locus_tag="OO326_003964"
     CDS             1712..2539
                     /gene="tauC"
                     /locus_tag="OO326_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5968140.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2536..3387
                     /gene="tauD"
                     /locus_tag="OO326_003965"
     CDS             2536..3387
                     /gene="tauD"
                     /locus_tag="OO326_003965"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5968141.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3493..4467)
                     /gene="hemB"
                     /locus_tag="OO326_003966"
     CDS             complement(3493..4467)
                     /gene="hemB"
                     /locus_tag="OO326_003966"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5968142.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1162 a         1277 c         1436 g         1205 t
ORIGIN      
        1 ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc gtggcgtatc
       61 aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct aaagagagcg
      121 gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg gcgctggctt
      181 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
      241 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
      301 taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc gtaccgttta
      361 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt aaaccagggc
      421 tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag cggggtgata
      481 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgc
      541 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
      601 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa agcgccatcg
      661 atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg gaaaacatca
      721 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggaa
      781 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
      841 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
      901 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
      961 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
     1021 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
     1081 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
     1141 ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg ctaccgtggc
     1201 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
     1261 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
     1321 atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
     1381 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
     1441 accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
     1501 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcatccg
     1561 gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt gttgcgggag
     1621 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
     1681 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
     1741 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
     1801 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
     1861 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
     1921 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
     1981 gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc taagcccgac
     2041 ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac cgctggctta
     2101 tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct tactgatcta
     2161 tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa gcgcgcagca
     2221 ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
     2281 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc tgggtgtggg
     2341 ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag gatttatggt
     2401 tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg cggtgattgc
     2461 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc tgacgccctg
     2521 gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc gtatatcggc
     2581 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacagctt
     2641 taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat tacgccgcag
     2701 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
     2761 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccggataac
     2821 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc gattctggca
     2881 gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat tgcggcctat
     2941 gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga gcatgatttc
     3001 cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca acgctggcgc
     3061 gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca tccggtgagc
     3121 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga tgtcagcgag
     3181 aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc ggagtttcag
     3241 gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt gactcagcac
     3301 tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac gatccttggg
     3361 gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg cccccttggg
     3421 gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc cccctttctg
     3481 agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa
     3541 aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga ctttctcttc
     3601 atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc tcacctgata
     3661 cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga ggtacgctcc
     3721 agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg actcacgaat
     3781 cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt ttaatgcgct
     3841 tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg aatacgacat
     3901 aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct gtacctggcc
     3961 gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa ccacggcttg
     4021 cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca
     4081 gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca tttctggcac
     4141 agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc aggcgtcgct
     4201 gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga taccggcgtt
     4261 ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca cgcctggcat
     4321 ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca acaccaggtc
     4381 gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg
     4441 acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat taaggtgcac
     4501 agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt gacaagaaga
     4561 gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc atattcactg
     4621 aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt ttggttgtgc
     4681 tgctataaat tgaataattc aaactttgtt gtatattgat aagatattta tctggtttgt
     4741 atttttgata tttataacgc gtatgcatca aaaataacat tatctgcaac atactgaaat
     4801 atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg tccacattcg
     4861 gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc gttagcgcga
     4921 aacataatat gttttctatg cattgataat tgatggatca acttattacg tccctgagga
     4981 gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag cattggcttg
     5041 cactctggct atcacctctc aggctaatgc agcgaccaac
//
LOCUS       DAJTRW010000186         4939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRW010000186 DAJTRW010000000
VERSION     DAJTRW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(162..746)
                     /gene="kptA"
                     /locus_tag="OO326_003967"
     CDS             complement(162..746)
                     /gene="kptA"
                     /locus_tag="OO326_003967"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5968143.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            996..1679
                     /locus_tag="OO326_003968"
     CDS             996..1679
                     /locus_tag="OO326_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5968144.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1676..2137
                     /locus_tag="OO326_003969"
     CDS             1676..2137
                     /locus_tag="OO326_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5968145.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2150..3322
                     /gene="iadA"
                     /locus_tag="OO326_003970"
     CDS             2150..3322
                     /gene="iadA"
                     /locus_tag="OO326_003970"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5968146.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3387..4298
                     /gene="hypT"
                     /locus_tag="OO326_003971"
                     /gene_synonym="qseD"
     CDS             3387..4298
                     /gene="hypT"
                     /locus_tag="OO326_003971"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5968147.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4291..4683)
                     /gene="iraD"
                     /locus_tag="OO326_003972"
     CDS             complement(4291..4683)
                     /gene="iraD"
                     /locus_tag="OO326_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5968148.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4680..4763)
                     /gene="idlP"
                     /locus_tag="OO326_003973"
     CDS             complement(4680..4763)
                     /gene="idlP"
                     /locus_tag="OO326_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5968149.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1167 a         1299 c         1228 g         1245 t
ORIGIN      
        1 ccagtcccca actgaccgcc tgccgtaaat cactttaatt tcagcagttg ttattaaagg
       61 ataccctttt acgagggtat cccttcagac tgataacaaa cgctacattg ccggatgtgc
      121 tgcgcttacc aggcctatat aacccctgca atatattgaa tttattgttt ttgtacccca
      181 gtcggcataa acgaccactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg
      241 ttttccgctt gccagaaagg gatgcctcgt ttcgccattt cctgtgcttt gacggttaag
      301 acaaccggcg atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga
      361 tgcacgtaat gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg
      421 cttgccgtac cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc
      481 tgcgaagtcg aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa
      541 cgctttttat cgctggttgc gaccacggta tccagcaatg cacgggttaa tcgcttgccc
      601 gctttttggg cacaaagtat gagcttattg atatccgccc atccttcacg gtccagtacg
      661 ataccaatcg cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc
      721 agctcttttt cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt
      781 actaatgtca ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gcagaacctt
      841 ttccacacag cgtaattttt cccctatgca aaaaacgcat agccagaaac atacttgcat
      901 tttccccata cccacatggc tgataaggtt tttcagttcc aggaattttc ctgactcaag
      961 caacacacaa acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag
     1021 ccggggagct ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca
     1081 taatcttttt tcccggtgtt ttttccggaa cagacagctg gtggcgcgtt tttgattttt
     1141 ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa tggagccacc acctcatttc
     1201 gcggggtggg tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt
     1261 cagtcattct ctctctgggg attatttcaa ttaccgatgg tcttggcgga ttacgcgccg
     1321 cgcagcagtt aatgacacct gtgctaaaac cgttgttggg cattccaggg atctgctcgc
     1381 tggcactgat cgctaacctg caaaacacgg atgccgccgc cggtatgacc aaagagctgg
     1441 cgcaggaagg tgaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg
     1501 gtagcgccat catcaccaac tacttctctt ccggcgtcgc ggtgtttgcc tttctgggca
     1561 cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt
     1621 tgcgcgtctg gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac
     1681 aactcaggtc cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc gtggctttac
     1741 tatcgccacg accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct
     1801 gaaaattacc ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg
     1861 gggattaccc ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc
     1921 agtcggtgtt gccgccagtc tggcaaccgc tgacgcatta accggccacg atgtcaccgt
     1981 tttactgccc gcaatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg
     2041 gacagccgaa gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc
     2101 attgctgtcg atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta
     2161 taccgcagcc ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg
     2221 aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca atatcccttc
     2281 tgacattgta ccgaactgca cggttgtcga tctcagcggg cagatcctct gcccaggttt
     2341 tattgatcaa cacgtccatt tgattggcgg tggcggcgaa gcaggtccca cgacgcgcac
     2401 gccggaagtg gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct
     2461 gggcaccgac tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa
     2521 tgaagaaggc atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcactat
     2581 tacgggttcc gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgcgc
     2641 catctctgat caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcaga
     2701 atcccgcgtt ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga
     2761 cagtaaaaag gcgttaaagc ctatttatga cctgctggaa aactgcgatg tgccgatcag
     2821 caagctgctg ccgacccacg ttaaccgcaa cgtaccgttg tttgagcagg cgctggagtt
     2881 cgcgcgcaaa ggcggcacca tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc
     2941 cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc
     3001 cgacggcaac ggtagccagc cgttcttcga tgacgaaggg aatttaaccc atatcggtgt
     3061 tgcaggtttt gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagact atgatttcag
     3121 tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acatgagcgg
     3181 gaaaggcgaa attctgccag gcaatgacgc agacttactg gtcatgacgc cagaattgcg
     3241 cattgagcag gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa
     3301 aggaacgttt gaaacggctt aaacggttgt cagccgccct aatggacgta gaatgccccc
     3361 aagggcggct gacagagtaa aacgtaatgg atgactgtgg tgcggttttg cataatattg
     3421 aaaccaaatg gctttatgat tttctgactc tggaaaaatg ccgcaatttt tcccaggcgg
     3481 cagtcagtcg caacgtctcg caaccggcat tcagccgccg catccgtgca ctggaacagg
     3541 cgattggcgt tgaattgttt aaccgccagg tgacgccact gcaactctca gaacaaggga
     3601 aaatcttcca ttcgcagatc cgccatctgt tgcaacagtt agaaagcaac cttgcagagc
     3661 tgcgtggcgg cagcgattac gcgcaacgta aaatcaagat agccgctgca cactctcttt
     3721 ccctcggtct gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg
     3781 aagccattga tgtcgatgaa gcggtcgata aactgcgtga agggcaaagt gactgtattt
     3841 tttcctttca cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat
     3901 ctcaattgtt ccctgtctgc gccagtgacg aacacggaga agcacttttt aacctcgcgc
     3961 agccacactt tccgttactg aattacagcc gcaactccta catggtgcga ttgattaatc
     4021 gcaccctgac gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg
     4081 agcttttaaa gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtatgcca
     4141 tccaacaaga aattcgcagc gggcagctcg ttgtgctgaa tcgggacgaa caggtgatcc
     4201 cgattcaggc ctacgcatac cggatgaata cccgcatgaa tccagttgcc gaacgcttct
     4261 ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctcaagcg tcgcactgcg
     4321 ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt ggtacagcat
     4381 tatctcaagg ctgatcgcca gagtgtggca gtacgcttct ttcaaattaa cctgcaacgc
     4441 cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg
     4501 gagatgtttg tcgctcgggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga
     4561 cggtaatgcc gaatgaggag aattaaaaag agtgaggaga tcgctgcata ccgatttacg
     4621 cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat
     4681 cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca
     4741 ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct
     4801 gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact
     4861 gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaacgct
     4921 agtatattct gaatattcc
//
LOCUS       DAJTRW010000187         4934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRW010000187 DAJTRW010000000
VERSION     DAJTRW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO326_003974"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO326_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5968150.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO326_003975"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO326_003975"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5968151.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO326_003976"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO326_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5968152.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO326_003977"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO326_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5968153.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>4934
                     /gene="yidZ"
                     /locus_tag="OO326_003978"
     CDS             4378..>4934
                     /gene="yidZ"
                     /locus_tag="OO326_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5968154.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLR"
BASE COUNT         1120 a         1185 c         1235 g         1394 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaa
//
LOCUS       DAJTRW010000188         4818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRW010000188 DAJTRW010000000
VERSION     DAJTRW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            186..370
                     /locus_tag="OO326_003979"
                     /pseudo
     CDS             186..370
                     /locus_tag="OO326_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            513..734
                     /locus_tag="OO326_003980"
                     /pseudo
     CDS             513..734
                     /locus_tag="OO326_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(755..817)
                     /locus_tag="OO326_003981"
                     /pseudo
     CDS             complement(755..817)
                     /locus_tag="OO326_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1267..2679
                     /gene="ospC1"
                     /locus_tag="OO326_003982"
     CDS             1267..2679
                     /gene="ospC1"
                     /locus_tag="OO326_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5968155.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2783..2932)
                     /locus_tag="OO326_003983"
                     /pseudo
     CDS             complement(2783..2932)
                     /locus_tag="OO326_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3008..4705
                     /gene="ospD3"
                     /locus_tag="OO326_003984"
     CDS             3008..4705
                     /gene="ospD3"
                     /locus_tag="OO326_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5968156.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1673 a          867 c          901 g         1377 t
ORIGIN      
        1 aaaaaatcat cttcgtgaag cttcacgaca tagcggggaa gtttgcttct ttgagaggcg
       61 ggtttacgtt tacgggattt agctgaacgg gccatataac cacctgaaag acaatgacat
      121 tgcctgtttt tataacggta attgcagacc atgacaaacc gcagccgtca ggctgcctac
      181 tcgagtcaca aggctctttg tgtccgatat gccggcagcg cattacccat gaaactggct
      241 ggaacgtaca ccataaggtg aagaaagtta tgggggcggt gaagaattat ctaaccttgt
      301 cttgttacat ccaaattgcc atcgacagtt acacagttgt gaagccggct ctctcaaaaa
      361 gggcttataa aggcttgagc ggtgtaccgg gaaaccggta tgcaccgttc ttaggggagg
      421 gcagtccagc aatgaactgt ccttacccga caaacatcca aaacgaacgg aacgtgctgg
      481 aatccgcata taacccgttg taaagtgagg aaatggacac ctcacttgct catgagaacg
      541 cccgcctgcg ggcactgttg cagacgcaac aggacactat ccaccagatg gctgaataca
      601 accgcctgct ctcacagcgg atggcggctt atgcttccga aatcaaccga ctgaaggcgc
      661 tggttgcgaa actgcagcgt atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa
      721 ccgaacggca gattccattt tcacgggcga tctacgccac acaggctctg ggttgcagtg
      781 actgctccat taaggcgatt ttgaactcat aagggaagta acaaaaaaac tgcaaaagta
      841 caataattat atatggccaa actaacctct attaaaaaac atcagttttt cacacttagt
      901 tgagcttcat gaaaaaatca tcgaggattt cattgcagaa tacacattcc actaactgga
      961 agcactccat aagaggaatc agagaaagta tcgttttttt atagtaaaat tcattgctgt
     1021 tcaataaaat tgatatagtt attaaattat gaaaataatt tcacaaaaaa gattaacttg
     1081 agttcttaag catcggattt tagatatttt gaaatatctg gcatcacata acatcaaaag
     1141 tatataatac tcttatctaa ttacatgcag tatattgtat atatactcaa aaattgtaat
     1201 tcataccctg ctatatcttg tagggaaaga tattaattaa aactgttttc atataaggtt
     1261 cattttatga atatatcaga aacactgaac tcagcaaata cccaatgcaa tatagattct
     1321 atggataaca gattacatac attgtttcca aaagtgacat cagtgcgaaa cgctgcacaa
     1381 caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa atattattaa aagtttcttt
     1441 aggaaaacta tagcagcaca gagttatagt agaatgttct ctcaaggctc taactttaaa
     1501 tctttaaata tagcaattga tgcaccatca gacgctaaag cctcatttaa ggctattgag
     1561 caccttgaca gattatcgaa gcattatata tctgaaataa gggaaaaact tcatcctctt
     1621 tctgcagagg aactcaattt gctttcgcta attattaatt ctgatttaat cttcagacat
     1681 caaagtaatt ctgatttgtc tgataaaatt ttaaacatta agtcattcaa taaaattcag
     1741 tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg atataaaaaa aatagctaat
     1801 catgactttg tgttttttgg cgttgaaatc tctaaccatc agaaaaaaca ccccctgaat
     1861 acaaaacatc acactgttga ttttggtgca aatgcgtata tcattgatca tgactctcca
     1921 tatggatata tgacattaac cgatcacttt gataatgcta ttccacctgt tttttaccat
     1981 gagcaccaat catttttaga taaattttca gaggttaata aagaagttag tcgatacgta
     2041 catggaagta aaggaattat agatgtacca atattcaata ctaaagatat gaagttaggg
     2101 ctcggattat acctgattga ctttattaga aaaagtgaag accaaagctt caaggagttt
     2161 tgctatggaa aaaatcttgc ccctgtggat ctggatagaa tcataaactt tgtttttcag
     2221 ccagagtacc atatacctag gatggtaagt acagaaaact tcaaaaaagt taagattaga
     2281 gaaatatcct tagaggaggc tgttacagca tctaattacg aagaaattaa caagcaggtc
     2341 actaacaaaa aaattgctct ccaggctctt tttctttcga ttactaatca aaaagaggat
     2401 gtcgccttat atatattatc taattttgag ataactagac aagatgttat ttccataaag
     2461 catgagttgt atgatattga gtatctactt agcgctcata attcaagctg taaagtactt
     2521 gagtatttta tcaataaggg attggttgat gtaaacacaa agttcaaaaa aactaatagt
     2581 ggggattgta tgttggataa cgcaataaaa tatgagaatg cagaaatgat aaaactatta
     2641 ttgaaatatg gtgcaacatc tgacaataaa tatatttaat caaaattgaa tatcgtttag
     2701 caaaaggcag tatttgcaca aaatatcgat atattgttta ttgtcagtat ggctcaatgt
     2761 gataatagtt ggaaagtttg atgggtttcg ccccgttgta gcggtagtcg aactggaagg
     2821 tcttcaggca ctgcttacag cgatagagca gcccccctga actggaatgg ccgttccgga
     2881 tacccccctg agtttcagag taacggtgac aaaccacatc aatctttgcc atcaatcatc
     2941 caaagggcaa agagtacaac aacactaagt ctgcgtcaca acccatcaat gaaaggaata
     3001 tatacatatg ccatcagtaa atttaatccc atcaaggaaa atatgtttgc aaaatatgat
     3061 aaataaagac aacgtctctg ttgagacaat ccagtctcta ttgcactcaa aacaattgcc
     3121 atatttttct gacaagagga gttttttatt aaatctaaat tgccaagtta ccgatcactc
     3181 tggaagactt attgtctgtc gacatttagc ttcctactgg atagcacagt ttaacaaaag
     3241 tagtggtcac gtggattatc atcactttgc ttttccggat gaaattaaaa attatgtttc
     3301 agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt atttattttg ttgaaaacgg
     3361 ttcatgggga gatattattt tttatatttt caatgaaatg atttttcatt ccgaaaaaag
     3421 cagagcacta gaaataagta catcaaatca caatatggca ttaggcttga agattaaaga
     3481 aactaaaaat gggggggatt ttgtcattca gctttatgat cccaaccata cagcaactca
     3541 tttacgagca gagtttaaca aatttaactt agctaaaata aaaaaactga ctgtagataa
     3601 ttttcttgat gaaaaacatc agaaatgtta tggtcttata tccgacggta tgtctatatt
     3661 tgtggacaga catactccaa caagcatgtc ctccataatc agatggcctg ataatttact
     3721 tcaccccaaa gttatttatc acgcgatgcg tatgggattg actgagctaa tccaaaaagt
     3781 aacaagagtc gtacaactat ctgacctttc agacaatacg ttagaattac ttttggcagc
     3841 caaaaatgac gatggtttgt caggattgct tttagcttta caaaatgggc attcagatac
     3901 aatcttagca tacggagaac tcttggaaac ttctggactt aaccttgata aaacggtaga
     3961 actactaact gcggaaggaa tgggaggacg aatatcgggt ttatcccaag cacttcaaaa
     4021 tgggcatgca gaaactatca aaacatacgg aaggcttctc aagaagagag caataaatat
     4081 cgaatacaat aagctgaaaa atttgctgac cgcttattat tatgatgaag tacacagaca
     4141 gatacccgga ctaatgtttg ctcttcaaaa tggacatgca gatgctatac gcgcatacgg
     4201 tgagctcatt cttagccccc ctctcctcaa ctcagaggat attgtaaatt tgctggcctc
     4261 aaggagatat gacaatgttc ccggacttct gttagcattg aataatggac aggctgatgc
     4321 aatcttagct tatggtgata tcttgaatga ggcaaaactt aacttggata aaaaagcaga
     4381 gctgttagaa gcgaaagatt ctaatggttt atctggattg tttgtagcct tgcataatgg
     4441 atgtgtagaa acaattattg cttatgggaa aatacttcac actgcagacc ttactccaca
     4501 tcaggcatca aaattactgg cagcagaagg cccaaatggg gtatctggat taattatagc
     4561 ttttcaaaat aggaattttg aggcaataaa aacttatatg gaaataataa aaaatgaaaa
     4621 tattacacct gaagaaatag cagaacactt ggacaaaaaa aatggaagtg attttctaga
     4681 aattatgaag aatataaaaa gctgaatatt atctcgtggt gatacactga atttgaggta
     4741 accggctcat ttaaaccgtc tggtctgtta cctccggttc cacaaaaata atgtccatca
     4801 ttttgaagtg gacactat
//
LOCUS       DAJTRW010000189         4696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRW010000189 DAJTRW010000000
VERSION     DAJTRW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            38..4156
                     /gene="pic"
                     /locus_tag="OO326_003985"
     CDS             38..4156
                     /gene="pic"
                     /locus_tag="OO326_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5968157.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4307..4549)
                     /locus_tag="OO326_003986"
                     /pseudo
     CDS             complement(4307..4549)
                     /locus_tag="OO326_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4588..>4696)
                     /locus_tag="OO326_003987"
     CDS             complement(4588..>4696)
                     /locus_tag="OO326_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5968158.1"
                     /translation="VDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1246 a         1072 c         1264 g         1114 t
ORIGIN      
        1 cttttatatc ccttgtaaac atcatggaga atccatagtg aataaagttt attctcttaa
       61 atattgcccc gtcaccgggg ggcttattgc tgtctctgaa cttgcccgca gggtaataaa
      121 aaagacatgc cgaagattaa cgcatattct tctggctggc attccagcaa tctgtctgtg
      181 ttactctcag atatcccagg cgggtattgt ccgttccgat attgcctatc agatttatcg
      241 tgatttcgcc gaaaacaaag ggctttttgt acctggtgcc aatgatattc cggtatatga
      301 taaggacgga aaacttgtgg gaagactggg taaagcccca atggccgatt tcagcagtgt
      361 gagctcaaat ggcgttgcta cgcttgtatc gcctcagtat atcgtcagcg taaagcataa
      421 cggaggatat cggagtgtga gctttggtaa tgggaaaaat acatattccc ttgttgaccg
      481 taataaccac ccttctattg acttccatgc tccacgtctg aataaactgg ttacagaagt
      541 tattccctca gcggtaacat cagaaggaac caaagccaat gcttataaat acactgaacg
      601 ttacaccgct ttttatcggg tgggtagtgg tacgcagtac actaaggaca aggacggaaa
      661 tttagttaag gttgccggtg gatatgcttt taaaacagga ggaaccacag gagttcctct
      721 gatatctgat gcaacaatag tctctaatcc cgggcaaact tataatcctg taaacggccc
      781 tttacctgac tatggagccc ctggggatag tggttctcct ttgtttgctt atgataaaca
      841 acaaaaaaaa tgggttattg ttgctgtatt aagagcatat gcaggtatta atggtgctac
      901 gaactggtgg aatgtcatac caacagatta tctgaaccag gttatgcagg acgatttcga
      961 tgcccccgta gactttgttt ccggactggg ccccctgaac tggacatacg acaaaacatc
     1021 aggcacaggt accctgagcc agggcagtaa aaactggacc atgcacgggc agaaagacaa
     1081 tgacctcaat gccggtaaaa atctggtatt cagcgggcag aatggtgcaa ttatcctgaa
     1141 agacagtgtg actcagggtg ccggttatct cgaatttaaa gacagttaca ccgtatctgc
     1201 tgaatccgga aaaacatgga cgggtgccgg cattattact gacaagggga cgaatgtaac
     1261 ctggaaggtc aacggcgttg ccggtgacaa cttgcataag ctgggggaag gaaccctgac
     1321 cataaacgga acaggtgtaa acccgggagg actgaaaacg ggagacggta tcgttgtact
     1381 taaccagcag gcagacactg caggtaatat ccaggccttc agttcagtga acctcgccag
     1441 cggacgtccg accgtggtgc tcggggatgc ccgtcaggtc aatccggata acatttcatg
     1501 gggataccgg ggaggtaagc ttgaccttaa tggtaatgcc gttaccttca cccgactgca
     1561 ggctgctgat tacggggcgg tgattacaaa taatgcacag caaaaatccc agcttttact
     1621 ggatcttaag gctcaggata caaatgtcag tgaaccgacg attggaaata tatccccctt
     1681 tggtggtacc ggcactccag gaaacctgta cagcatgata ctcaacagcc agacccgctt
     1741 ctatattctg aaatctgcca gctatggtaa cactctgtgg gggaacagcc tgaatgatcc
     1801 ggctcagtgg gagtttgttg gcatgaacaa aaacaaagca gttcagacag taaaagatag
     1861 gatcctggcc gggcgggcaa aacaacccgt tatctttcat ggtcagctga ccgggaatat
     1921 ggatgtcgcc attccacagg tgccgggggg aagaaaggta atctttgatg gtagcgtgaa
     1981 cctgccggaa ggtaccctga gtcaggacag tggcaccctg atattccagg gacatccggt
     2041 tatccatgcc tccatcagtg gcagtgcacc ggtcagcctg aaccagaaag actgggaaaa
     2101 ccgtcagttt acaatgaaaa cactgtcgct gaaagacgct gacttccatc tttcacgtaa
     2161 cgcctcgctg aacagtgaca ttaagtcgga taacagccat atcacactgg gaagtgacag
     2221 ggcatttgtg gataaaaatg acggaacagg aaattatgtc attccggagg aaggtacctc
     2281 tgtcccggac accgtgaatg acaggagcca gtatgaaggg aatattacgc tgaaccataa
     2341 ctcagccctg gatatcggca gcaggttcac cggggggatt gacgcttatg acagtgccgt
     2401 cagcatcacc tctccggacg tcctgttgac agccccgggt gcttttgccg gcagttcact
     2461 gacagtgcat gatggcggtc atcttacagc actgaacggt cttttcagcg acgggcatat
     2521 tcaggccggt aagaacggca aaatcaccct gagcggtaca ccggttaaag atacggctaa
     2581 tcagtatgcc cctgctgtat atctgacgga cggatatgac ctgaccggcg ataacgcagc
     2641 actggaaatt acccgtggag cacatgcttc cggtgatatt catgcctctg cggcatcaac
     2701 agttaccatc gggtctgaca cgccggcaga actggcttct gcggaaacgg ctgcatcggc
     2761 gtttgccggc agtcttcttg agggctataa cgcagcattc aatggtgcca taaccggtgg
     2821 cagggctgat gtcagtatgc ataatgcact gtggactctg ggtggggact ctgccatcca
     2881 cagtcttacc gtcagaaaca gccgtattag ttctgaagga gaccgtacat tccgtaccct
     2941 gacggtgaat aaactggatg caacaggcag tgattttgtt ttgcgtacgg acctgaaaaa
     3001 tgccgataaa attaatgtga ctgaaaaagc cactggttca gataacagcc tgaacgtcag
     3061 ctttatgaat aatcctgctc agggacaggc cctgaatatt cctctggtca cggcaccggc
     3121 gggaacttca gcagagatgt ttaaggccgg cacccgggtg acaggtttca gtcgggtgac
     3181 cccaaccctg catgttgata ccagtggtgg caatacgaag tggatactgg atggttttaa
     3241 agcggaggct gataaagccg ctgccgcgaa ggctgacagt ttcatgaatg ccgggtataa
     3301 aaacttcatg acggaagtta acaatctgaa caaacgtatg ggtgacctgc gtgacacaaa
     3361 cggtgatgcc ggtgcctggg cgcgcatcat gagtggtgcc ggttctgcag acggtggtta
     3421 cagtgataat tacacccatg ttcaggtcgg ctttgacaaa aaacatgaac tggacggtgt
     3481 ggacctgttt accggtgtca cgatgaccta taccgacagc agtgcagaca gccatgcatt
     3541 cagcggaaag acgaaatcgg tggggggcgg tctgtatgct tcagcattgt ttgagtccgg
     3601 tgcctatatc gatttgattg gtaaatatat tcaccatgac aatgattaca caggtaactt
     3661 tgctagcctg ggaacgaaac actacaacac ccattcctgg tatgccggtg ctgaaacggg
     3721 ttaccgctat cacctgacag aggacacgtt cattgagccg caggctgaac tggtttacgg
     3781 cgccgtgtcc gggaaaacat tccgctggaa agacggtgat atggacctga gcatgaagaa
     3841 cagggacttc agtccgctgg ttggaagaac aggggttgaa ctgggcaaga ccttcagtgg
     3901 taaggactgg agtgtgacgg cccgtgccgg aaccagctgg cagtttgacc tgctgaataa
     3961 tggagagacc gtactgcgtg atgcgtccgg ggagaaacgg ataaaaggag agaaggacag
     4021 ccggatgctg tttaatgttg gtatgaatgc gcagataaag gacaatatgc gctttggtct
     4081 ggagtttgag aagtcagcct ttggtaaata taacgtggat aatgcggtaa acgcgaattt
     4141 ccggtatatg ttctgatgtg ggggtaaagg gctgccggaa ggcagccttt ctcagagact
     4201 cttaaaatat aaaactgaag gtcttcggta agggggagtg tattagacgt caactagttt
     4261 tgccgactgg cgcgagcctg tttaagattc tgtgtaaatg cctttctcag aagtgaccgt
     4321 ccaggcggtc accgaactcg ataataaagc ggctcattgc cattcgccag tccttcaacg
     4381 gcatcgtcca tttctgggac gcagactgga ttgccagcca caccactttt ttcaccgagt
     4441 cgtctgtcgg gaacacttta cgctttttga tcgcatggcg gatcacgctg tttagcgact
     4501 cgatggcatt cgtcgtatag atcactttgc ggatgtccgt tggataagcg gtaagcgtac
     4561 agcgagggcc gtattgacgg ggatgtgtta ttcagctggc agtgctatgc gccacggaag
     4621 cagttcgctg acccggttga ccggccagtc tgctatgacg ccaagcacat ggcgaaggta
     4681 gctttctgga tccacg
//
LOCUS       DAJTRW010000190         4693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRW010000190 DAJTRW010000000
VERSION     DAJTRW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(40..990)
                     /gene="uspE"
                     /locus_tag="OO326_003988"
     CDS             complement(40..990)
                     /gene="uspE"
                     /locus_tag="OO326_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5968159.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1142..1894)
                     /gene="fnr"
                     /locus_tag="OO326_003989"
     CDS             complement(1142..1894)
                     /gene="fnr"
                     /locus_tag="OO326_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5968160.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2089..2604)
                     /gene="ogt"
                     /locus_tag="OO326_003990"
     CDS             complement(2089..2604)
                     /gene="ogt"
                     /locus_tag="OO326_003990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5968161.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRAIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2615..2758)
                     /locus_tag="OO326_003991"
                     /pseudo
     CDS             complement(2615..2758)
                     /locus_tag="OO326_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3148..3963)
                     /locus_tag="OO326_003992"
     CDS             complement(3148..3963)
                     /locus_tag="OO326_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5968162.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4060..>4693
                     /locus_tag="OO326_003993"
     CDS             4060..>4693
                     /locus_tag="OO326_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5968163.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1246 a         1099 c         1112 g         1236 t
ORIGIN      
        1 ggcccgataa ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc
       61 cagttcaacg ggtgtctgat actggtcagg tttaatgacc agcaggtcgc agcgaagatg
      121 atcaatcacc tgttccgccg tgttgccgag gaatgctgct gaaataccgg tgcgtccgac
      181 cgtgcccaga accacaattc ccgcctgtaa atgctccgcc aaatcaggaa tcacctcttc
      241 gggcagacct ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct gccgcagggc
      301 tttcattgcc agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg ggtcaaattc
      361 cggcagttca atcgcgatat taattggcgt taccggataa gcgccaacca gatgaacttc
      421 ggtatggttg acttgttctg ccagttcgat cgtctctttg accagttttt cattgagcgc
      481 attatgatac ggctcttcac tggcgagatt caccgccacc agcgccttgc ctccttccgg
      541 ccacggctgg tctttcacca tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc
      601 cgttggcgta aaaatcaccg cttccagacg gtcatgttgg tgcgccattt ttagcaccaa
      661 atcgtgtccg ccgctgatca cttcctgaat gatggcttcg aaaggacggt tatgccagac
      721 cactttaatt tcaatgggaa cgccagcatt gagataatat tttgcctgct cgtggatcca
      781 ggctgtacgc tggctgatga cgccctgacg catagcggta cgttcgtccg gggagagcag
      841 ggtggtcatt tcgtatgaga agtcatagat cggcaaaaag gctttaattt tgccaccaat
      901 ccgttgatgt aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat
      961 aacaacgagc atgttctgat acatagccat acagggtctc cttacaacaa ctgtcaacgc
     1021 agtttgtaat taaaagatta acccatatct ggtgaatgaa acagtgatga accttctgcc
     1081 agatcaataa atcagaaaaa tttaatgata tgacagaagg atagtgagtt atgcggaaaa
     1141 atcaggcaac gttacgcgta tggccagcaa gctgggccag cgcatcgtta ttttcgatgg
     1201 tgatgtattt acctttgact gccagcatac cgcttttctg gaagcgaccc agcagacggc
     1261 tgatggtttc taccgtcagg cccagatagt taccaatatc gccacgagtc atcgtcaggc
     1321 ggaattcacg aggggagaag ccgcgttggg caaaacgacg ggacaggttg tagatgaatg
     1381 cagccagacg ttcctcggca tttttcttcg acaacagcaa gatcatgtcc tgatcgcctt
     1441 tgatttcacc gctcatcaga cgcatcatct gctgacgcag attcggcatt ttaccggaca
     1501 aatcgtccag cgtttcgaac ggaatttcac ataccatcga ggtttccagc gcctgagcga
     1561 agctcgggtg atggccgctg ccgatggcgt caaatcccac taggtcgcct gctaaatgga
     1621 aaccagtgat ttgctcgtcg ccttgctcag tgattgtata acttttaatc gtaccggagc
     1681 ggatggcata aagcgattta agttcatcac cagccttaaa cagcgtctgg cctttctgaa
     1741 taggcttctt ccgctcaatg atattatcaa gctgatcaag ctcatgttcg ttgagtgtga
     1801 acgggatgca aagctggctg atgctgcaat cctggcaatg gatagcacaa ccgccagact
     1861 gaatgcgccg tataattcgc ttttccggga tcataggtct gctcaagccg taattgatat
     1921 ttgtcaattt taacatcttt ttagggagca agtaagtcta agcaaacctt aacagcagag
     1981 aattccgata ttagatgtaa atatatgtct atctatttga aactccttaa gttgtcaagg
     2041 gtaactttac ataaaagtgt gaacaagctg gcacaaattg tttaatgttt acagcaaaag
     2101 ataaccttcg tggcgcaata accactcttt tcgctgaact ccgcctgcat atccggtcat
     2161 ggtgccgttt cggccaataa cccgatggca aggtacgacg atgctgatgg gattcgatcc
     2221 gtttgccgca ccaacggcac gcgccgcgcc agggcggccc aattgttcag ccagttggcc
     2281 gtaatgcatt acctgcccgc aggggatagc gcgtagtgtt ttccagactt cgcgctgaaa
     2341 tggcgtcccc cccgtggcag tgggaagcgt atcaataatg ctaagattac cggcaaaata
     2401 ttcacgaagc ttgtcgctta aaccacctgg gttggtggca gaaatgcgct catagccttc
     2461 tttgcgataa tggatgtcca gcagctgcac catgcgttcg ctgtactctt cccattcaac
     2521 cgcccgcagg cgaaattgct catcgcaaat cacccacagt ggacccagtg gcgtggcaat
     2581 tttttcttca agtaatctca gcatccgttc tctcttaaga caaacgtggg taaataccct
     2641 gacctatcgg cagaccgaca agataccacg ccagcaacat cagcagccat accaccaaaa
     2701 agataagtgg atagggcaag actaacgaat agtaagtacc cagtttcgcg tctggtttgt
     2761 cgcagtctga tagcataaag cgatttttta atttttctgc ctgaagaaat gagggcaagc
     2821 catcacatgg atcgagggtg ggataattcc agtcagtgaa attttaccgt aggctatctg
     2881 ttatttgatt ctgtggcgtg atagccgcca gacaccactg aactcgtcac tcattcccca
     2941 atcccggcga gaatgtggtt atttacagag ctaaaaaatg aataaaaaaa tagagcctgt
     3001 cgacatccgc cagactctac agtacacaca gcagtgcatc ctcgtcttaa atccggcctg
     3061 gggtcctggt gaccggtgac agtgttataa cggttttacc catccgttgc caattttctt
     3121 cagtgacaga tttcacgaaa atataagtta aatcatttgt cttgtgattg cataattatg
     3181 catcgttaat cgacgagtaa atttaccggg cgagtagccc gttcgacgct taaacatatt
     3241 gccgaacgat tcagctgagg catagcccag acgatgggca attttggtca acgaatatcc
     3301 atcatcaaga agcgtcactg cagaaataac gtgcatttgc tgaacccagt ggcgaaatgt
     3361 taatcccgtc tctttaacaa ataagcgcat gagtgtacgg ctactgacgt taattttatc
     3421 cgctagatta tccagtgttg gcagataccc ttggttgttt agcaaattct cacaaagctg
     3481 ctgtagccgc tcatcatttg gccaagggag ttcaagtgac attttcaccc ccatttttat
     3541 ttcctgaagt atcagtgtct cgagggctga aaaacgtaac tgttgtttat ctgtggtact
     3601 ttgcgacggt ggaagttggt taattgcaat tagtaattcc cttaacaggg gtgaaatacc
     3661 cgtcatgaag caaactgttc ccagcgtttc actttccgct tctgaaacca gtacggtttt
     3721 taaaagtacg ttgcctgtcg caacaaactt atgcggcatt tgtgggggca gccaaagcgc
     3781 gctgctggca tggactagcc agacgctttc tccagcccat acacgaacga ctccttcagc
     3841 cgggtaaagc aactgggcat agagatgttt atgccaggga actgtacctc cattcagatg
     3901 ttccgaatcc agtccgataa tgtgatgccc tggtgatgct ttgggttgca gtgtaattgt
     3961 catttacagt aggtattttg tcagaaatca tcaaataagc catgttaaat tttaaatatg
     4021 acgtatgcaa gtttccggat atatacggtg taaagagtga tgataatttc taaaaaacag
     4081 cttattgggg tggtagcgat cggtattctt ttgactggcg ttgtcttttt tatctggagg
     4141 gtaagcaaag gaagatttat acagacgacg gatgatgctt acattggtgg taatatcacc
     4201 acggtggcaa gtaaagtttc tggttatatt tctgctatag aggtgcgcga taatcagagc
     4261 gtaaaaaaag gcgatattat cctgcgtctc gatgatcggg attatcgtgc aaatgtcgcc
     4321 aggcttgagg caaaaataaa aagtagcaaa gccaatctcg aaggcattca agccactatt
     4381 acgatgcagc agtcgataat tcaaagtgcc agtgaaacat ggcaggcagt aaaacatgag
     4441 gagcaaaagc gcctgcgaga tactgaacgt tatgaaaaac tcgcacaatc agcggcaata
     4501 tcgcaacaaa ttattgataa cgccaggttt gactaccagc aggtcgcagc aaaggagcgc
     4561 aaagccgcga acgatttcct ggtggagaag caacggctgg cagttttatc tgctcaggaa
     4621 gaaaacgtac gtgcgagtat cgaagaagtt caggccgctt tgactcaggc actactggat
     4681 ctggaatata ccc
//
LOCUS       DAJTRW010000191         4691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRW010000191 DAJTRW010000000
VERSION     DAJTRW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..416
                     /locus_tag="OO326_003994"
     CDS             <1..416
                     /locus_tag="OO326_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5968164.1"
                     /translation="QSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFE
                     WNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQ
                     AQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(617..1474)
                     /gene="sitD"
                     /locus_tag="OO326_003995"
     CDS             complement(617..1474)
                     /gene="sitD"
                     /locus_tag="OO326_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5968165.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1471..2328)
                     /gene="sitC"
                     /locus_tag="OO326_003996"
     CDS             complement(1471..2328)
                     /gene="sitC"
                     /locus_tag="OO326_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5968166.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2325..3152)
                     /gene="sitB"
                     /locus_tag="OO326_003997"
     CDS             complement(2325..3152)
                     /gene="sitB"
                     /locus_tag="OO326_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5968167.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3152..4066)
                     /gene="sitA"
                     /locus_tag="OO326_003998"
     CDS             complement(3152..4066)
                     /gene="sitA"
                     /locus_tag="OO326_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5968168.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4498..>4691)
                     /locus_tag="OO326_003999"
     CDS             complement(4498..>4691)
                     /locus_tag="OO326_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5968169.1"
                     /translation="IRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
BASE COUNT         1262 a         1139 c         1154 g         1136 t
ORIGIN      
        1 tgcaaagcgg caaatggggt gagatagccc cattcactgt gacaccagaa atgctggaag
       61 cggccagaga ggccagacgt caggaaattg aagcatggcg cgcagaacag gaggcgaagc
      121 tgttcacgtt tgaatggaac ggtcgtatct ggaatgctgg tcccgactca ctgggccgcc
      181 tgtccccggt agtcatgctg gcaaaatctg tcacagcaca aacacatatg gcgtggagcg
      241 atgccgataa tcagcaggtg aaactgtcga tgccggaact ggaagaactg gcggcagcaa
      301 tggtgcaggc gcaggtcgat cgcaacgatg agatttatcg ccgtcagcgg gagctgaagg
      361 aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg gaataacaga
      421 agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt tcgatagtgc
      481 gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg atagaattcg
      541 tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc ggacgatgct
      601 gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg tttccctttg
      661 tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg aataagacga
      721 caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca gtcattgaca
      781 ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt gtcaggagta
      841 ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct actgctttca
      901 gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc catctcgtat
      961 gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa tcccgccatt
     1021 ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt atgtcgccga
     1081 tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct ggtttaattg
     1141 caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc ataactgtat
     1201 cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag aatactccgg
     1261 cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg atgccaggaa
     1321 aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc agaaagaccg
     1381 acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga ctcataaagg
     1441 tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg ctttatgcgc
     1501 acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta aaagaaacag
     1561 cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt aactgaccca
     1621 cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca ggcggggaaa
     1681 acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga tcaccagaaa
     1741 ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga agaagaggat
     1801 ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga aaaaggtcac
     1861 cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa tgatagtgag
     1921 ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg tctgaatgtt
     1981 cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg acgagaagat
     2041 caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca gcatgctgcc
     2101 cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca gcatatatgc
     2161 ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg accagccttt
     2221 tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg agacccacat
     2281 cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct tgcttccctc
     2341 tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat aatgctttct
     2401 tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc caggttctcg
     2461 gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac catgaccgta
     2521 taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat cgttttgcct
     2581 tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac atcaacgccg
     2641 gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct cgccagaaag
     2701 acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa atccaccata
     2761 tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc tatacgcaac
     2821 atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac aggaaatgac
     2881 cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag cgcctgtcgc
     2941 gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc cataatcgct
     3001 ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat agatccactt
     3061 ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt gacagtaaca
     3121 tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg gctttaattc
     3181 cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt accgggccgt
     3241 tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg gtttcacgcg
     3301 caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc gggatatgat
     3361 ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt tgatcggcat
     3421 taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa gaaaaagccc
     3481 cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc ttacgcaggg
     3541 gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg taggtttgtg
     3601 catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa atcagagcat
     3661 tatctggcga catccatgca tggggattag gtttgccctc atagggtcct tcggtgatcc
     3721 ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga tgctggtaaa
     3781 agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt gccccctgcg
     3841 cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca ggcttggtta
     3901 tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg atgatggtaa
     3961 atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc gagcaggtga
     4021 gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt tagtacctat
     4081 agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa cctatacaaa
     4141 atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt tgtttcctgc
     4201 tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa tgactttatg
     4261 aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta gcttattgtt
     4321 gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt cagatagttg
     4381 tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt gttgattttc
     4441 ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac tttgcaacta
     4501 cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa tccacatacc
     4561 gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta taaatgccgg
     4621 gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt acggattacg
     4681 gcggcgaata c
//
LOCUS       DAJTRW010000192         4687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRW010000192 DAJTRW010000000
VERSION     DAJTRW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(163..951)
                     /gene="phoH"
                     /locus_tag="OO326_004000"
     CDS             complement(163..951)
                     /gene="phoH"
                     /locus_tag="OO326_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5968170.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1558..2823)
                     /gene="efeB"
                     /locus_tag="OO326_004001"
     CDS             complement(1558..2823)
                     /gene="efeB"
                     /locus_tag="OO326_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5968171.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2829..3956)
                     /gene="efeO"
                     /locus_tag="OO326_004002"
     CDS             complement(2829..3956)
                     /gene="efeO"
                     /locus_tag="OO326_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5968172.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4014..>4687)
                     /locus_tag="OO326_004003"
     CDS             complement(4014..>4687)
                     /locus_tag="OO326_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5968173.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1100 a         1315 c         1134 g         1138 t
ORIGIN      
        1 gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca gaacctcgct taggcctgtg
       61 tccatattac gtgggtagga tcagtttcag aaaaagcgct agtccgggca taaacaccca
      121 aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt atgcatgcag
      181 cgtacgttgg caaagtgccg aacgtacgca atcctctttt ccgaaccgga cgatcccaac
      241 catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg
      301 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
      361 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacaactgc
      421 attttcaaag gtacgtccac gcatataggc gaacggcgcg gtttccacct tccctatttc
      481 cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca agtcgtagac
      541 cgggcgaaaa tagggagcaa acttttctgc gatatcacca ggtaagaagc caagatcttc
      601 atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc
      661 ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa
      721 tatcagctgc ttactctcaa tagcctgcag atagtgcaat tgcgcttcat ttcgcgcaag
      781 gatgggcgag gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgctcatctg
      841 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
      901 cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca tggagagcac
      961 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
     1021 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
     1081 acgcacaccg tttgcggcgt cggtaacacg agtaaagaaa ggaagtgaga aaaatacaga
     1141 gtgacgaagt tgctaccgga ggagaaatct ccgcgagtgg tggagcgttg cttaacaaaa
     1201 cattgtccag aacaggacgt taccatccgc gatctccata gtgactgact atcactgccg
     1261 ggaacttccg ctgctactta ataagtacaa cagaactcgc attcattgca acaatatatt
     1321 cacttatatt taacgataac acccatttcg gtgacattaa tttctactgg aaagatgaca
     1381 gagtgatgac agtgatggaa aaagctgtgt gctttccgca ggattcgcag ggctggtaaa
     1441 atgtgcctcg tgtttgccgg attcggcgcg aacgccttac ccgacctaca agtgattaat
     1501 aatttgtgca caaaactcat gcccgataaa cctgcgcatc gggcatattt gcgtcgttca
     1561 aacctgcaat agcgcccttc ccagataatc gttcgcgtcc ttcactcccg gcagcgaaaa
     1621 aaaataaccg ccgccgatag gtttaacgta ttcctccagc gcttcgccat tgagcctttt
     1681 ttgtactgtc aggaagcctt tttccagatc gtgttggtag cagacaaaca gcaaccccat
     1741 atccagttgt ccggagttgg tgacccccaa cgaataactg tagccgcgac gcagcatcag
     1801 gctggactca ctctccgccg tgcggggatt cgccagccgg atatggctgt ccagcgcgat
     1861 caccttccct tccgggtcgc tggcgtaatc aggcacatca tgctcatgct gcattcccag
     1921 cggcgcaccg gtttgcttat cacggccaaa aatcgtctgc tgttctttca gcggcgttct
     1981 gtcccaaaat tccactcgaa actgaatcaa gcgtactgcc tgatagctgc caccgattgt
     2041 ccacgcaggc tcctgctgat ctgccgttac ccacaccact ttttgcatca acttatcatt
     2101 ctggctatcg ggattggcag tgccgtcttt gaaacccagc aaattaatcg gcgtctcttt
     2161 gcctttacta cgcgccgcgt gatcggaaat aaacccttcc cgcttccagc gcacactgag
     2221 caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg ttcaccgtgt cctgggtgtt
     2281 ggcgcaaatc tgtagcaaca catcaccatg acataacgcc gcatccagcg agtcgttggg
     2341 gaaacgcgtc atcttctgca gcttttttgg catctgtggc gcaaggccaa agcgctcatc
     2401 aaacaatgag tgacccaccg ataacgtgat ggtgagatta tcgggcgcaa tgtagccgcc
     2461 aagaatgccg gaatcgagtg gtggcaggcg cggatttggc gtttctggtg ctgctccgcc
     2521 ctgactcaga aaagcaaaac gctgagtcaa caagcgaaac aaccgctcaa gatcggcttt
     2581 atcgctggca agcacatcaa acgccaccag catcattgcg gcctgttgtg gcgtcaggat
     2641 ccctgcctga tgctcaccat aaaacggctg tttctcattg cgtgcatccg gtgaaagcgt
     2701 gcccggtgca ctttgcgttt tttgtgcatg agcgaccgga caacttcccg ccagcgcacc
     2761 aatccctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat ctttatactg
     2821 cataacgctt aatccagtcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca
     2881 gtaatcggcc ctttcagcgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa
     2941 ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact
     3001 tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc gacaattttc
     3061 tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct
     3121 tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc gccgaccact
     3181 tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa
     3241 agctgctcag cgtactgatc catccctttg gtggtgttgt cgccaaacaa tgctttttcc
     3301 agacggtgga aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc atcttcacgg
     3361 gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat acgctcatag
     3421 tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg
     3481 tcggtaaagg ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta
     3541 tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt
     3601 gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg
     3661 tattcgccag gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc
     3721 cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg
     3781 ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc
     3841 ttatccgtca cggtcacttt gacctgcggc acatcagcgg cgttagccat aaaagcagaa
     3901 gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg
     3961 agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg
     4021 gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg
     4081 agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata gccaaaaatc
     4141 ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat atcgaaggcg
     4201 atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc accagccgcc
     4261 agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg
     4321 aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc aagaccgagc
     4381 attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag
     4441 accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca
     4501 tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac tttgacgttg
     4561 cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc
     4621 acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg
     4681 aagatcc
//
LOCUS       DAJTRW010000193         4565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRW010000193 DAJTRW010000000
VERSION     DAJTRW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..475
                     /locus_tag="OO326_004004"
     CDS             <1..475
                     /locus_tag="OO326_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5968174.1"
                     /translation="WHIPHEKIWIEDQSTNCGENARFSITLLNQAVERVHTAIVVQDP
                     TMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSL
                     LTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            672..2111
                     /gene="aldA"
                     /locus_tag="OO326_004005"
     CDS             672..2111
                     /gene="aldA"
                     /locus_tag="OO326_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5968175.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2153..3153)
                     /gene="gap"
                     /locus_tag="OO326_004006"
                     /pseudo
     CDS             complement(2153..3153)
                     /gene="gap"
                     /locus_tag="OO326_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3342..3872
                     /gene="cybB"
                     /locus_tag="OO326_004007"
     CDS             3342..3872
                     /gene="cybB"
                     /locus_tag="OO326_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5968176.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4117..4290
                     /locus_tag="OO326_004008"
     CDS             4117..4290
                     /locus_tag="OO326_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968177.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4362..4511)
                     /gene="hokB"
                     /locus_tag="OO326_004009"
     CDS             complement(4362..4511)
                     /gene="hokB"
                     /locus_tag="OO326_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5968178.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1167 a         1050 c         1151 g         1197 t
ORIGIN      
        1 ctggcatatt ccgcatgaaa aaatctggat tgaagaccag tcaacaaact gcggtgaaaa
       61 cgcacgcttt agcatcacgc tattgaatca ggccgtagaa cgagttcata cggctatcgt
      121 tgtacaggac cccaccatgc agcggcgcac gatggcaacg ttccgccgta tgaccgggga
      181 caatcccgat gcaccacgct ggttaagtta tcccggattc gttcctcagt taggaaataa
      241 cgcagacagt gtaatcttta ttaatcagtt acaaggatta tggccggttg agcgttatct
      301 ctcactactc actggcgagc tgccgcgttt acacgatgat agcgatggct acggtccccg
      361 cgggcgagat tttatcgttc acgttgattt tccggcagaa gtcatccatg catggcaaac
      421 gctgaaacat gatgcggtgc tcatcgaggc gatggaaagt cgctcgttac gttaaaaatt
      481 gcccgtttgt gaaccacttg tttgcaaacg ggcatgactc ctgactttta tttctgcctt
      541 ttattccttt tacacttgtt tttatgaagc cctccacaga cttgtccttt cccgatgccg
      601 tctctctgat gattgatgtt aattaacaat atattcaccg aaaacaaaca tataaatcac
      661 aagagtcgcc catgtcagta cccgttcaac atcctatgta tatcgatgga cagtttgtta
      721 cctggcgtgg agacgcatgg attgatgtgg taaaccctgc tacagaggct gtcatttccc
      781 gcatacccga tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca gaacgtgcac
      841 aaccagaatg ggaagcgttg cctgctattg aacgcgccag ttggttgtgc aaaatctccg
      901 ccgggatccg cgaacgcgcc agtgaaatca gtgcgctgat tgttgaagaa gggggcaaga
      961 tccagcagct agctgaagtc gaagtggctt ttactgccga ctatatcgat tacatggcgg
     1021 agtgggcacg gcgttacgag ggcgagatta ttcaaagcga tcgtccggga gaaaatattc
     1081 ttctgtttaa acgtgcgctt ggtgtgacta ccggcattct gccgtggaac tttccgttct
     1141 tcctcattgc ccgcaaaatg gctcccgctc ttttgaccgg taataccatc gtcattaaac
     1201 ccagtgaatt tacgccaaac aatgcgattg cattcgccaa aatcgtcgat gaaataggcc
     1261 ttccgcgcgg cgtgtttaac cttgtactgg ggcgtggtga aaccgttggg caagaactgg
     1321 cgggtaaccc aaaggtcgca atggtcagta tgacaggcag cgtctctgca ggtgagaaga
     1381 tcatggcgac tgcggcgaaa aacatcacca aagtgtgtct ggaattgggt ggtaaagcac
     1441 cggctatcgt aatggacgat gccgatcttg aactggcagt caaagccatc gttgattcac
     1501 gcgtcattaa tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta cagaaaggca
     1561 tttatgatca gttcgtcaat ctgctgggtg aagcgttgca ggcggttcaa tttggtaacc
     1621 ccgctgaacg caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg
     1681 ttgagcaaaa agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag
     1741 cggtagaggg gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggaaa
     1801 tgtcgattat gcatgaggaa acctttggcc cggtgctgcc agttgtcgca tttgacacgc
     1861 tggaagatgc tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata
     1921 cccaaaatct gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca
     1981 tcaaccgtga aaacttcgaa gctatgcaag gtttccacgc cggatggcgt aaatccggta
     2041 ttggcggcgc agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt
     2101 tacagtctta atgagtgaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt
     2161 tagcgaattt ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc
     2221 aggcgaccgt tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat
     2281 caaacactga accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat
     2341 aaccaaatga ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag
     2401 tcactttttt ccgagaatgg acaccagctc agtgaccgaa cctgttttca ccggcacgcg
     2461 ttgcgcatga cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc
     2521 cgcccccgta gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt
     2581 accacgcggg ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt
     2641 gccgacttct atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt
     2701 ggtgcatgac gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattaacgtt
     2761 ataaacgatg gttttcattt caccggcagg agcggaaatc aacaccttct tcgcaccagc
     2821 atcaagatgc gcctgcgatt tatcggcgga ggtataaaag ccagtacatt cgacaatgat
     2881 ttctgcccct ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat
     2941 acttttccca tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaacggacc
     3001 atagtttgaa tcatgtttca gcagataggc gagaattttt ggggaagtaa gatcattaat
     3061 agcgacaacg tctatgttgc ttttgacttc aagtaaacga cgcaacacca gtcgaccgat
     3121 acgaccaaaa ccgttaatac caactttact catggttttc tcctgtcagg aacgttcgga
     3181 tgaaaattga tcctttccag gcttagacca ggattgtggg atgggcaatc tccattctta
     3241 cagtgaaacg taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc
     3301 ccgttatgtt aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa
     3361 ggttacaaat cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa
     3421 tggagtttcg tggtttcttc ccacgtagcg atcggccgct catcaacatg attcatgttt
     3481 cctgtggcat ctcaattctc gtgctgatgg tggttcgtct tttgttaaga ctgaaatacc
     3541 caaccccgcc gattatacct aagccgaaac cgatgatgac gggactggcg catttgggac
     3601 atttggtgat ttatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata
     3661 accggggcaa cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt
     3721 tcgaacgggt tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg
     3781 tcatcgggtt gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac
     3841 ttctacgcat gatgccgcgt aaacgttcct gaaggatatt taaagaaaac gcctgtacta
     3901 aaaccgaccc gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt
     3961 atttgtatga caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat
     4021 caattcaccc gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca
     4081 aatcgcacat aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg
     4141 tttatgggaa cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg
     4201 aaagggggga tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt
     4261 agtgcatcga aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa
     4321 tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc
     4381 atgagcgcag caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg
     4441 gtcaggagtg tgaatgccag aatcgtaatg cagataatga gcagacacac caccagaggg
     4501 ttgcgcttca tagcctttct ccttgccgga tggcgggtaa gaggctaaca atctgaattg
     4561 cttgg
//
LOCUS       DAJTRW010000194         4268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRW010000194 DAJTRW010000000
VERSION     DAJTRW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..1222
                     /locus_tag="OO326_004010"
     CDS             <1..1222
                     /locus_tag="OO326_004010"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5968179.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO326_004011"
     CDS             1222..1956
                     /locus_tag="OO326_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5968180.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="OO326_004012"
     CDS             2423..3184
                     /locus_tag="OO326_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5968181.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3228..>4268
                     /locus_tag="OO326_004013"
     CDS             3228..>4268
                     /locus_tag="OO326_004013"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5968182.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFK"
BASE COUNT         1171 a          820 c         1048 g         1229 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaa
//
LOCUS       DAJTRW010000195         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRW010000195 DAJTRW010000000
VERSION     DAJTRW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO326_004014"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO326_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5968183.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO326_004015"
     tRNA            complement(324..399)
                     /locus_tag="OO326_004015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO326_004016"
     tRNA            complement(406..480)
                     /locus_tag="OO326_004016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO326_004017"
     tRNA            complement(597..681)
                     /locus_tag="OO326_004017"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO326_004018"
     tRNA            complement(690..765)
                     /locus_tag="OO326_004018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO326_004019"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO326_004019"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5968184.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO326_004020"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO326_004020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5968185.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO326_004021"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO326_004021"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5968186.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJTRW010000196         4057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRW010000196 DAJTRW010000000
VERSION     DAJTRW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..389
                     /locus_tag="OO326_004022"
     CDS             <1..389
                     /locus_tag="OO326_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5968187.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO326_004023"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO326_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5968188.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO326_004024"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO326_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5968189.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO326_004025"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO326_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5968190.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO326_004026"
     CDS             3116..3487
                     /locus_tag="OO326_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968191.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            3546..4057
                     /locus_tag="OO326_004027"
                     /pseudo
     CDS             3546..4057
                     /locus_tag="OO326_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1065 a          790 c          880 g         1322 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgagag ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
     3541 ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc
     3601 gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat
     3661 ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata
     3721 tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt ggcctcaaca
     3781 cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg
     3841 cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg gggctaaatc
     3901 gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg ttgcgcacgt
     3961 attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga
     4021 cgtggtgata tggatgacgg atggctggcc gctgtat
//
LOCUS       DAJTRW010000197         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRW010000197 DAJTRW010000000
VERSION     DAJTRW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(886..1077)
                     /locus_tag="OO326_004028"
     CDS             complement(886..1077)
                     /locus_tag="OO326_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5968192.1"
                     /translation="MTKQEKTALNMTRFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO326_004029"
     CDS             1142..1465
                     /locus_tag="OO326_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5968193.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO326_004030"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO326_004030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO326_004031"
     CDS             2143..2673
                     /locus_tag="OO326_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5968194.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO326_004032"
     CDS             2680..2913
                     /locus_tag="OO326_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5968195.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO326_004033"
     CDS             complement(3459..3851)
                     /locus_tag="OO326_004033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968196.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          916 c         1207 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggtcatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTRW010000198         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRW010000198 DAJTRW010000000
VERSION     DAJTRW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..596
                     /locus_tag="OO326_004034"
     CDS             <1..596
                     /locus_tag="OO326_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968197.1"
                     /translation="LFIMAICMAMVLLFLYINKDNTKVIYSLKINQTTPGILVSCDSN
                     NNFACQTTMTEDVIQRITTFFQTSPDIKNREIRLEWSGDKRDLPTAEEEISRVQASII
                     KWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNK
                     YPAAICLALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(839..1534)
                     /locus_tag="OO326_004035"
     CDS             complement(839..1534)
                     /locus_tag="OO326_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5968198.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1527..2954)
                     /locus_tag="OO326_004036"
     CDS             complement(1527..2954)
                     /locus_tag="OO326_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5968199.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2965..3684)
                     /locus_tag="OO326_004037"
     CDS             complement(2965..3684)
                     /locus_tag="OO326_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5968200.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1065 a         1062 c          845 g         1070 t
ORIGIN      
        1 tcctttttat tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag
       61 acaatacaaa agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta
      121 gctgtgatag caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc
      181 gaattactac attttttcag accagcccag atatcaagaa cagagaaata aggctggaat
      241 ggtcaggaga taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt
      301 ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac
      361 aaacgccttc agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact
      421 ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata
      481 aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta atttctgtaa
      541 tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg cagtaataaa
      601 cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg gaagggaacc
      661 cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt actaatgcct
      721 ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag gttgtcctta
      781 acgctatttt caacctcgta tgccttcttc aggtttatgt tcagatctca tatctctctc
      841 agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg acgactttga
      901 taagctcaat atccgccgtt gagctgggac cactgatgat gttaatacat gacggcattc
      961 gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca cgcggcagga
     1021 ttgtgctttt acgcaggata aaaagagaag attccgggag caggctcaat gaacgcccgc
     1081 gctcggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat tcagcataca
     1141 cgacacccac tttagcctgc tctgcctgcg agatattctc ggcacctttc gccggatccc
     1201 aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc tccagcctcg
     1261 tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt attgcagctt
     1321 ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg ctggcaaact
     1381 gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg ttagcatagt
     1441 tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc agtgcctgag
     1501 caacgttatt caaaaattcg ctccgattat ccattctttt tctcctgcgc ctgatgtttc
     1561 ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc ttccatccag
     1621 tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca gcttgccgca
     1681 tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc ataagcgaac
     1741 atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat cacccgacga
     1801 tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc acaagcagtg
     1861 cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag tagcggagaa
     1921 atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc aatatggcga
     1981 tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac atcccgaaat
     2041 tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa ctcttcagga
     2101 ccatcaacgt gccccgcttc gcgcggccct gtcagccagg tgttgtatcc cgtcaaacgt
     2161 gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc aaacgtgggg
     2221 gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt acacattcgc
     2281 gcattacctt cattggtcac caggcatacc gaaccggtct ctgccaccgc gaaattacag
     2341 ccggtaatac ctatttcagc actgaggaaa tcttcacgga ttttttgccg gatgaataag
     2401 gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag cactcgacgg
     2461 atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg atcttgatcc
     2521 agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc atcctgcaac
     2581 acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac cttccgggca
     2641 tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt ttttgcaaaa
     2701 tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata agcgtcgaga
     2761 ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc ccagtgcccc
     2821 aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgct tgccaccgct
     2881 ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc tgtattactg
     2941 gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa tatgcatcac
     3001 tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc aactcacgtc
     3061 agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt ctttcaccat
     3121 ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc agcaggtatc
     3181 ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg taagtggctc
     3241 gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag ctctcccttg
     3301 caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg tgagatcctg
     3361 catacgcgcg gcaacctttt cggcacgtga tgcccattca ggttcatccg ccagatacgt
     3421 cgggtaactt tttatggcat aggtgcaaga gccagccggt gaaataatgg gatcgtcgtt
     3481 atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga tataaccgct
     3541 attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc gacagccgag
     3601 tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt caccaataca
     3661 ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc atttatgccc
     3721 gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt ttttaattgg
     3781 ctagcaacat accacccctc atttgtatga caaatgataa tctttcatcc aaacttcaaa
     3841 tgcaataact aattttattt ctttcggcat cgctcacatt ttatggggaa atcgacgatc
     3901 atttttggca taagaaatcc atttaaaaat ggctacatcc cttataccta ttcagagcta
     3961 cttattcagg ggttattgag atatacactg atggtgtaca aaaaaatcac cgacaataat
     4021 cacgtatcaa gtaatagtcg ga
//
LOCUS       DAJTRW010000199         3997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRW010000199 DAJTRW010000000
VERSION     DAJTRW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            33..338
                     /locus_tag="OO326_004038"
     CDS             33..338
                     /locus_tag="OO326_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968201.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            565..1728
                     /locus_tag="OO326_004039"
     CDS             565..1728
                     /locus_tag="OO326_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5968202.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            2077..3249
                     /locus_tag="OO326_004040"
     CDS             2077..3249
                     /locus_tag="OO326_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5968203.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(3303..3926)
                     /locus_tag="OO326_004041"
     CDS             complement(3303..3926)
                     /locus_tag="OO326_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5968204.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT         1263 a          843 c          837 g         1054 t
ORIGIN      
        1 gtaatgccaa aagcaccgga ggctggagca taatgaccac ttttaccgac aaagaactga
       61 ttaaagaaat taaagagcgt atcagcagcc ttgacgtgcg agacgatatt gagcgccgtg
      121 cttatgaaat cgcactccta tctctggaag tagaaccaga tgaacgcgaa gcttatgaat
      181 tattcatgga aaagcgtttt ggtgacttag tagatcgtcg gagagcaaaa aacggcgata
      241 acgaatacat ggcatgggat atgactctcg gttggatcgt ctggcagcaa cgagctggta
      301 tccatttctc aacaatgtca cagcaagagg tgaaataatg gagccataca gcctcacact
      361 cgatgaggcc tgtcattttc tcaagatatc cagaccgact gccattaact ggatacgcac
      421 agggcgtctt caggcaacac gcaaagatcc cactaagaat aaatctcctt acctcacaac
      481 acgacaagcc tgcattgcgg ctcttcagtc tccgctgcat actgtccagg tgagcgcggg
      541 tgatggcata acagaggaaa gaaaatgtca ctcttccgca gaggtgaaat atggtacgcc
      601 agtttcacat tgccgaacgg taaaagattt aaacagtctc ttggaacaaa ggacaaaagg
      661 caggcgacag aactccatga caagctaaag gctgaagcat ggcgggtcag caaacttggt
      721 gaaatacctg atataacgtt cgaggaagcg tgtgtcaggt ggcttgaaga gaaagcacat
      781 aaaaaatcac tggacgatga caaaagccgg atcggattct ggcttcaaca tttcgcagga
      841 atgcaactaa gagacattac tgaatcaaaa atttattcag caatgcagaa aatgacgaac
      901 cggcgtcatg aggaaaactg gagactcagg gcagaagcat gcagaaaaaa agggaaacct
      961 gttccagaat acacgccaaa accagcgtcc gttgcaacga aggctacgca tctttcattt
     1021 ataaaggccc tactaagagc cgcagagcgt gaatggaaaa tgctggataa ggcaccaatt
     1081 attaaagtgc ctcaaccaaa gaataaacgg atccgctggc tggagcccca tgaagcacaa
     1141 aggctgattg atgaatgtcc ggagccatta aagtctgttg ttgaatttgc actggcaaca
     1201 ggcttaagac gctcgaacat catcaacctt gaatggcaac aaatagatat gcagcgccgg
     1261 gtggcatgga taaacccgga agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat
     1321 gatactgcat gtcgcgtatt gaaaaaacaa atcgggaatc atcaccgttg ggtatttgtg
     1381 tacaaggaaa gctgtaccaa accagacgga acgaaagcgc caacggtcag gaagatgcgg
     1441 tatgacgcaa acacagcctg gaaagcggcg ctgagacgag caggtattga tgatttcaga
     1501 tttcacgact tgagacacac ctgggcaagt tggctggttc aagccggagt cccgttgtca
     1561 gtgttacagg aaatgggagg ctgggagtct atcgaaatgg ttcgtcgata tgctcacctt
     1621 gcacctaatc accttaccga acacgcacgg caaatagact cgatcctgaa cccatcggtc
     1681 ccaaattcgt cccagtcaaa aaataaggaa ggtactaatg atgtgtaact tattgattta
     1741 aatggtgccg ataataggag tcgaacctac gaccttcgca ttatgaatct gtagcaccaa
     1801 tcataactat ctgttttagc aagcattaac cgcattcact aagctatagt tgatgacaca
     1861 aacagaaagt tgatgcatga tgttgccata tatatgtcac aaatacggca caacgatctt
     1921 caaacatata gccacacatt cacagaagag cacaaagcct tgcaatccag tgcaaagctt
     1981 ggtgtgtctc cgttttgttt catcagttac acataactat atgatcatta actaaaagtc
     2041 agtatacttg gagaaatttt aattgcgtaa tagagcatgc atatgattga aattaacagt
     2101 ttattattaa taacatccgt gatattgatg tcgttattag ctgtggggtt atttgataaa
     2161 atttccccaa taaatcttgt tgagcacggc aggaataatc aaatagatgg aatgcggggg
     2221 ttcttagcaa ttttcgtgct tattcatcac gcagcaattt ggaatggata cttgtcatct
     2281 ggagtatggg aagcaccttc atcaaatctg ttagcaaatt taggccaagt tggtgtgtca
     2341 ttctttttta tgattactgg ttatctgttc ttttcaaaga ttatctctgg agatcaggac
     2401 tggacaaggc tttatgtatc aagattacta cgattaaccc caatgttcat agttagccta
     2461 tgtctcattt ttatcattgt aggttttaag tctggatgga gaatgcaggt atccacagaa
     2521 gagctttttg tgtcaataat gaagtggttg ccattcactg cgctaggtat gccgaacatt
     2581 aatgacgtaa aagattcatt tactatcaat gccgctgtaa catggacact tgtatatgaa
     2641 tggttctttt atttttctct tccggtaatt tccgcgctca taaaaagaaa agtcagtatt
     2701 tacatggtta tgattagcgc aatatcgcta tttgttttca ttttattttt cagtaaaata
     2761 cacatagcat catttctttt tgggctcctg gcatttttgc taaataaatc aaagatagtc
     2821 aatggaattg ccaaagcaaa agtaacacca attataataa ctgcgataat ggtttttgaa
     2881 atgacgtatt tcaaaacaac ttacgcaccg ctaccactga ttctttgtgg aataacattt
     2941 atcattatcg catcaggttg tgatttgtat ggaatattaa gattaaatat aaccagaaag
     3001 ctaggagaaa ctacctacag cgtttacctt ctgcatggga tattccttta ttgcctaatg
     3061 acgtggatta ttcctaataa ttacactgaa aatacattta tcatactggt aagcacaact
     3121 gcatttctta ttacgttcac atcatgccta acttttaaat taatagagac gccattcatc
     3181 aaattaacaa aacaaaccac aactttagta aaagaattaa tacccacatt aacaaacaac
     3241 aatcaataaa caataaacat aagccgccct acatatatgt ccaaatgggg cggcgaatgt
     3301 atttatatta gtctggtaat gctaaatgca aacgaactgg agattcctgc cccgctaagc
     3361 gtattgcgca gggtcaccct aaatgtatct acgccaatat aagtaacatc acaactaact
     3421 gaatcaggcg cagatatagg tgttatctgc acaatataac cgctagcaac tgctgcgagc
     3481 ggatgagaaa agctaacatc atacacacct gtagaaacca cgttaaccga tgaaacacca
     3541 gtagcaacgt cgatactacc attggcgtta atttttcccg agcgtgtgac tgacaatgca
     3601 gaagccgatg ttaccttact ggttgtttct gtaaaaacac acccaatgtc aactacctcg
     3661 caattagctc ctgttatcca gtatggtcgt tctgcacttg gctgataatc ccctgcgctc
     3721 tggaacgttg tgccgttgag tatgactgta acttttcccc cacctgagct tgttacttga
     3781 atattcgtgt gcgtgtatct tgcactgctc acgcgatgaa aatttccacc gtttataacc
     3841 acagttacag ggcgcgtccc agtattatct atagcaaggt ctgccccccc ggcattagcc
     3901 tcaaagtatg ggttgtttaa taccaaagcg cacgaaccgt taaggccatt tatagctaat
     3961 tgcgcaccac ctgtactgga atccccctga gtaccat
//
LOCUS       DAJTRW010000200         3958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRW010000200 DAJTRW010000000
VERSION     DAJTRW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..221
                     /locus_tag="OO326_004042"
     CDS             <1..221
                     /locus_tag="OO326_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5968205.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OO326_004043"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OO326_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5968206.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OO326_004044"
     CDS             515..1105
                     /locus_tag="OO326_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5968207.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OO326_004045"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OO326_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5968208.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OO326_004046"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OO326_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5968209.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OO326_004047"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OO326_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5968210.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OO326_004048"
     CDS             3454..3645
                     /locus_tag="OO326_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968211.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1070 a          982 c          986 g          920 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa
     3721 aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa cggaaccgac
     3781 gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc
     3841 cctcaaaatg gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca
     3901 gcggctcatg catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaa
//
LOCUS       DAJTRW010000201         3941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRW010000201 DAJTRW010000000
VERSION     DAJTRW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(135..296)
                     /locus_tag="OO326_004049"
     CDS             complement(135..296)
                     /locus_tag="OO326_004049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968212.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(293..481)
                     /locus_tag="OO326_004050"
     CDS             complement(293..481)
                     /locus_tag="OO326_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5968213.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            741..1076
                     /gene="iraM"
                     /locus_tag="OO326_004051"
     CDS             741..1076
                     /gene="iraM"
                     /locus_tag="OO326_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5968214.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1357..1465)
                     /locus_tag="OO326_004052"
                     /pseudo
     CDS             complement(1357..1465)
                     /locus_tag="OO326_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1988..2064)
                     /locus_tag="OO326_004053"
     tRNA            complement(1988..2064)
                     /locus_tag="OO326_004053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Arg,seq:tct)
     gene            complement(2166..2241)
                     /locus_tag="OO326_004054"
     tRNA            complement(2166..2241)
                     /locus_tag="OO326_004054"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2206..2208),aa:Ile,seq:cat)
     gene            complement(2386..3207)
                     /locus_tag="OO326_004055"
     CDS             complement(2386..3207)
                     /locus_tag="OO326_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5968215.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3222..3578)
                     /locus_tag="OO326_004056"
     CDS             complement(3222..3578)
                     /locus_tag="OO326_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5968216.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3591..>3941)
                     /locus_tag="OO326_004057"
     CDS             complement(3591..>3941)
                     /locus_tag="OO326_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5968217.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1117 a         1057 c          756 g         1011 t
ORIGIN      
        1 atccggaagc tgacaacaat aaaaaagcca ccagttaact actgatggct ctgataactc
       61 atgcaagcgt ctcagacgac ccactgacac taccggtgag tttaacgata ccttccattt
      121 gactggctca ctttttatga tgatgccggt gcatttatct ccagcaccag actttctatc
      181 tcaacgccat acgttgcatt tttggtaata tccgtcagcg tcagtgcatt tagtcccact
      241 gccagactgt cttttatggc ctggaatgcc gggccagtac gatgacgtag tatcactccg
      301 gctcagttgc accactgacc accacatcac cttctgctgc aatcgcctgc atcagggtat
      361 aaggggttat ggccaccgga ctaccaaacg gctgccagcc ctctttcagt ttatgtgtca
      421 gcttttccgc aagatctgac ggcggcgccg ccctgacaac atcatagtgt ttaaatgcca
      481 tggttctttc caacatctga aaaataattc tttaaaatac ctgacatgta atacagaaaa
      541 aacacaaaac cataccttaa ataaaaacct gattatcaag cagatatgca tggataaact
      601 acaagacgag atataaacca ccctgcattt aaataaacaa taaacaacat cagaaaaata
      661 attctgttct atggtttaat tcaaaaatat catttatact tttcagaaca tcaccagcaa
      721 ggcataaaca aggaaactaa atgaagtgga ttgtgattga tacagttatc cagccatcat
      781 gcggaatatc tttttcagtc atatggagta aaataaaatt aataatctgg tatcaatcgg
      841 atgctttctt acctcctgaa agtatattta cactgactca cacaggcatc atgctcaata
      901 acaaagtgct acctgtaacc atttacaacg tagtaccatt caataaaaca ttctggaatt
      961 taatcaaaaa cagccaggaa tgccctacaa atacagataa cgtattgaat gaatgcttta
     1021 ataaccgttg cactctgcaa atatgccctt atggactaaa acaacaaagt ccataaggag
     1081 tttactcaca tctgacaaaa tcaatataaa cagcccctcg ggagaggggc tggagagtgg
     1141 cgctatgtgc cattccatgg tgccgggtgc ctcccggtga attcagtacc agcacctgaa
     1201 tccgcgatta tcccatatac ctactcgctg attgcccctc cgcacagggg gattcaccat
     1261 gccagtttct tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc tgaattgtga
     1321 agtatttaaa aatttctccc gctaactgat acccggctaa cagtctggcg ttttcttttt
     1381 cagcaacggg aaagcaacaa ccaccacacc cgccaccagc acaccgtcag ccggcactga
     1441 cattatccgg ctgctgcaat gccattaaca aaaacagtaa gcaatcactt tttaccgtaa
     1501 caggtgataa tccagatatg tatctacccc agatgagtaa tccgaagttc atccatacca
     1561 caggtcctgg ctattctgtt gtactcctga acaagagcaa ataattctga attagcaacc
     1621 atgaactcat cgcaaaccct ctgtatagca tcactattca gaataataac gtctcttccc
     1681 gaaagacgat caggagtaca gaacaaaact gtcaaacggc tgaaggcctt tgctcgtgct
     1741 gcattgacta tatcaatacg ctgcctaagg atgaaacacc ccgacgcctc atcaatattc
     1801 actctaccca caccatatga atgataaata cttaatgctg aaaaaaccat tagaccgtat
     1861 aacaaatact caatcaatac ttaacagaac ttttattttt gacaaatata taatattttc
     1921 aacaatatcc tgagccaggt atatttcagt ataaggctct gccggaagga atctggaaga
     1981 atgaatatgg cgcgctgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc
     2041 tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg gggacccgcc
     2101 ccccgcacca acaaccctgt tatcgtgtcg tctgctcttc ctgataagct aatggcggtt
     2161 tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc
     2221 tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta atttcgcaat
     2281 aaaaaaaccc gctcagcggc gggttgtaga aactcttcta acgccaggca taaaacgccc
     2341 atcattatga cgaatttacc acagattccg gaaaaatcaa ccttgttacc tagttacctt
     2401 ttttaactgc cgctcagccc atgcttcttc aatatcaaac cgggtcacca gcgcatcata
     2461 aaatttctta actgtttttt cccatgacgc gcgtgttatc tggtttgtca cctcgcatat
     2521 agcattaaat gcctccgttg atggtagtct ttcatagcca cgaccaccac aacgctggca
     2581 gtctctgata acaggcatac cacgttttac cgactcttca cgatgaatgg cgacaccacg
     2641 cccacggcaa tccttacagg cggtggaaac ctcacccttt ccgccacact ccggacaggc
     2701 aacttttacc acctccctga cttttttcca ttcttcccag taaaacggat acacaccttt
     2761 cgtacacttt gcccataccg gcggcttacc atccggatac tggatcttgt ttgtaaaaac
     2821 tatgctttca ataaattttt ccccatagca acaagggcac tgctttttac tcgctgcgct
     2881 gcgggcataa tcctcaaaag cgtacgaagc cataatgcgc atcactaccg gttttatttc
     2941 tgccggaagt tttctcaacg ccgccacacg atcgcaccga ctgagtgcat aatctgccag
     3001 taattctgtt gcccgcgccc tgtcattcat actgatgccc attttcccca ggaacgcaga
     3061 aaaacccatc tcagcccgat tctgtgtcat gccctgcgcg gccatcacat cagtgatact
     3121 cagcgcatct tttgacgttg aggccgatgc atcggtcagg ccaggggatt ttggggagta
     3181 gtatttcggt aaatcttcca gtttcatttt ttgacctgct cttaatgcat tatggggtaa
     3241 atcttcaccc ccagacgtcc accagatact ggctgaccac gaacgatatt gatttcatca
     3301 aactgctcat cgtccattaa cactcccgca tgcgtcagcg catccagcgg tgctttcagg
     3361 atattgtcca ggtcgcgacg acgcttatcc ggtggctctg caatcacctt tatcgccagc
     3421 cttccggaca ggcttaattt cagccgctgc tggcgaacaa taagcgccac agcccggcga
     3481 taacgctttc cctcctccga gataaaatat gtgctgccac ggcgtcgcca gtaagtgttc
     3541 accgtcggcg ggtaaggtaa aaccaaatct atgagcatca gtcacctctt ttacccaagc
     3601 acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg agaaccatgc
     3661 ttttcttcga acgccagagg atccgcatga agctcgttgt gatgctcccg acacagcggt
     3721 agcgtgaaaa tatcgtgaga tttggtcccc attccgccct gaccatgacc aatcaggtga
     3781 tggggatcgt cggctggctt accacaacac gcacacggct gtgtcttcac ccagcgtttg
     3841 tatttctcgt taacccagcg gcgacgttta ggtcgtttca tgaaagattc cggagactca
     3901 ggatcaacgg caatgctgac caccgtcttt tcctgttgtg g
//
LOCUS       DAJTRW010000202         3931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRW010000202 DAJTRW010000000
VERSION     DAJTRW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(76..2109)
                     /gene="metG"
                     /locus_tag="OO326_004058"
     CDS             complement(76..2109)
                     /gene="metG"
                     /locus_tag="OO326_004058"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5968218.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2241..3350
                     /gene="apbC"
                     /locus_tag="OO326_004059"
     CDS             2241..3350
                     /gene="apbC"
                     /locus_tag="OO326_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5968219.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3612..3893
                     /locus_tag="OO326_004060"
     CDS             3612..3893
                     /locus_tag="OO326_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5968220.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          963 a          975 c         1021 g          972 t
ORIGIN      
        1 agacaaaatc gcaagaaaac aactttagga atttataaag aaaaggcgct gtcgatgcag
       61 cgccttgaag ggggattatt tcacctgatg acccggttta gcaccggcat ccgggcttag
      121 caggaaaata tctttcccgc caggaccggc agccatcacc atgccttcag agataccgaa
      181 gcgcatttta cgtggtgcca ggttagccac cataatggtg tgacgaccaa tcagtgcctg
      241 cggatccggg taagcagaac gaataccgga gaagacattg cgtttttcac cgccgagatc
      301 cagcgtcagg cgcagcagtt tgtcagaacc ttcaacaaac tctgcgtttt cgatcagcgc
      361 cacgcgcagg tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa tcggatcatc
      421 tgccagtggg ccagtaactg gcgcggtagt ggctttcact tcttctttag aggcttccac
      481 cagagcttca acctgtttca tatcgatgcg gttatacagc gccttgaacg gattcacttt
      541 gtggcccagc agcggttgct ggataccatc ccaggtcagt tccgtattga ggaaggcttc
      601 tgcacgctcg gtcagtttcg gcagtacagg cttcaggtaa gtcatcagta cgcggaacag
      661 gttgatgccc attgagcaaa tcgcctgcag gtcagcatcg cgaccttcct gttttgcgac
      721 tacccacgga gcctgctcgt cgacatagcg gttagccagg tcagccagtg ccatgatttc
      781 gcgcactgct ttaccaaatt cacggctttc ccacgcttca ccaatcactt cagcggcatc
      841 ggtaaaggtt ttgtacagct cagggtcagc cagctcgctt gccagcacac cgtcaaaacg
      901 cttgttgata aagcccgcat tacgggaggc caggttaacc actttgttca cgatatcggc
      961 attcacacgc tgaacgaaat cttccaggtt gagatcgata tcatcaatgc gcgaagagag
     1021 tttcgcagtg tagtagtaac gcaggctgtc agcgtcaaaa tgattcagcc aggtgctggc
     1081 tttaataaag gtgccgcgag acttggacat cttcgcgccg ttcaccgtca catagccatg
     1141 aacaaacagg ttggtcggct tgcggaagtt gctgccttcc agcatggcag gccagaacag
     1201 gctgtggaag taaacgatat ctttaccgat gaagtggtac agctcggcgg tggagtcttt
     1261 cttccagtat tcatcgaagc ttacgctgtc gccgcgcttg tcgcacagat tcttgaaaga
     1321 acccatgtag ccaatcggtg cgtccagcca gacgtagaaa tatttgcccg gtgcgttcgg
     1381 aatttcaaaa ccaaagtatg gcgcgtcgcg ggagatatcc cactgttgca ggccggattc
     1441 aaaccactcc tgcattttgt ttgccacctg ctcctgcaac gcaccgctgc gggtccatgc
     1501 ctgcagcatt tcgctgaaag agggcagatc aaagaagaag tgttcagaat cacgcatcac
     1561 tggtgtagca ccagaaacca ccgatttcgg ctcgatcaat tcagtcgggc tgtaggtcgc
     1621 gccgcagact tcgcagttat cgccgtattg atccggggat ttacatttcg ggcaggtgcc
     1681 tttcacaaaa cggtccggca ggaacatgcc tttttccgga tcgtacagct gagagatggt
     1741 gcggttttta ataaaaccgt tttctttcag gcgagagtag ataagttctg acaactggcg
     1801 gttctcttcg ctgtgcgtcg agtgatagtt gtcatagctg atgttaaagc ctgcgaaatc
     1861 agtctgatgc tcctgactca tttcgccaat catctgctcc ggggtgatac cgagctgctg
     1921 agctttcagc atgatcggtg taccgtgggc atcgtcggcg cagatgaagt tgacctcgtg
     1981 gccgcgcatt cgctggtaac ggacccagac atcagcctgg atgtgctcca gcatatggcc
     2041 gaggtggatt gagccgttag cgtacggcag tgcgcacgtc accagaattt tcttcgcgac
     2101 ttgagtcata gtaggcatta cttcttaata gtgaaaaggg gttagatagt accaaatggg
     2161 aaaatgttaa gtaagtactt ctctatattc ttcaggtaaa ctatcagctg atggtttatc
     2221 tatacaaaaa aggagtcggg atgaacgaac aatcccaggc caaatcacca gaagccttgc
     2281 gcgcaatggt cgccgggaca ctggccaatt ttcagcaccc aaccctgaag cataacctca
     2341 ccacgctgaa agcgttgcac catgtcgcct ggatggacga cacgttgcat gtggaactgg
     2401 ttatgccgtt tgtctggcat agcgcgttcg aagagttaaa agagcaatgt agcgccgaac
     2461 tgctgcgcat caccggggcg aaagctatcg actggaaact gtcgcataac atagccactc
     2521 tgaaacgcgt gaagaatcag ccgggaatta acggtgtgaa gaacattatc gcggtgagtt
     2581 ccggtaaagg cggcgtgggt aaatcgtcca cggcggtaaa cctggcgctg gcactggcgg
     2641 cggaaggggc gaaagtcggc attctggacg ccgatatcta cggcccatca attcccacca
     2701 tgctgggcgc agaaaatcag cgtccaactt cgccggacgg tacccacatg gcaccgatta
     2761 tgtctcatgg cctggcaacc aattcaattg gttatctggt cactgacgac aatgcgatgg
     2821 tgtggcgtgg accgatggcg ggcaaggcgt taatgcagat gttgcaggaa accttgtggc
     2881 cggatctcga ctatctggtg cttgatatgc cgccaggcac cggtgacatt caattgacgc
     2941 tggcgcaaaa cattccagta actggtgcag tcgtggtcac caccccgcag gacatcgcgt
     3001 tgatcgatgc gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg gtgctcggta
     3061 ttgtcgagaa catgagtgtg catatttgca gtaactgtgg tcaccatgaa ccgattttcg
     3121 gtaccggtgg tgcagagaaa ctggctgaaa aataccatac acagctgctg gggcagatgc
     3181 cgttacacat ctccttacgt gaggatctcg ataaaggcac gccaacggtt atcagccgtc
     3241 cggagagcga atttaccgct atctaccgcc agctggctga ccgcgttgca gctcagctct
     3301 actggcaggg tgaagtcatt cctggcgaga tttccttccg cgcggtctaa cgcctattaa
     3361 ccattctggt tattcttcat acaccgcaga gatgttatat tgatgcggtg tatttgtttt
     3421 tgccgcatta atgaacactt atttaatact gcgaatattc tgcggacctt cgtccgaaac
     3481 tctacaaagt cgagtgatcg tacaggcaat gcttcattat catccttcgt gctgtgtagt
     3541 ggatgtacag ctatagtttg ttatgcactc attctatcca ggcataacct ttactacagg
     3601 gataacgttc aatgaataag tattggctgt caggaattat ttttcttgcc tatggattgg
     3661 cttctcccgc agtctcttca gagactgcca cattaacgat taatggcagg atcagtcccc
     3721 caacatgcag tatggctatg gttaatagtc aaccacaaca gcattgtggt cagttaacgt
     3781 ataatgttga cacccgtcat caggtttctt cgcccgtcaa aggtgttacg acagaagtgg
     3841 ttgttgcagg tagcgatagc aaacgtcgaa tcgtgttaaa tcgctacgat taatttggca
     3901 acctgatttt aattgcattt agaaagacga a
//
LOCUS       DAJTRW010000203         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRW010000203 DAJTRW010000000
VERSION     DAJTRW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            437..2089
                     /locus_tag="OO326_004061"
     CDS             437..2089
                     /locus_tag="OO326_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5968221.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO326_004062"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO326_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO326_004063"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO326_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO326_004064"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO326_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1051 a          879 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cccccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTRW010000204         3728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRW010000204 DAJTRW010000000
VERSION     DAJTRW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO326_004065"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO326_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5968222.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="OO326_004066"
     CDS             2168..3445
                     /locus_tag="OO326_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5968223.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          903 a          850 c          937 g         1038 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaa
//
LOCUS       DAJTRW010000205         3659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRW010000205 DAJTRW010000000
VERSION     DAJTRW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO326_004067"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO326_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5968224.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO326_004068"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO326_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO326_004069"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO326_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          892 a         1092 c          908 g          767 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaa
//
LOCUS       DAJTRW010000206         3628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRW010000206 DAJTRW010000000
VERSION     DAJTRW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            19..897
                     /locus_tag="OO326_004070"
     CDS             19..897
                     /locus_tag="OO326_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5968225.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO326_004071"
     CDS             894..2285
                     /locus_tag="OO326_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5968226.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO326_004072"
     CDS             2282..2539
                     /locus_tag="OO326_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968227.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO326_004073"
     CDS             2634..2834
                     /locus_tag="OO326_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5968228.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO326_004074"
     CDS             2827..3207
                     /locus_tag="OO326_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5968229.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3407..3483
                     /locus_tag="OO326_004075"
     tRNA            3407..3483
                     /locus_tag="OO326_004075"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3441..3443,aa:Ile,seq:cat)
     gene            3485..3561
                     /locus_tag="OO326_004076"
     tRNA            3485..3561
                     /locus_tag="OO326_004076"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Arg,seq:tct)
BASE COUNT          947 a          799 c         1047 g          835 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tgacatatgg ttattattct gcctccggcc ctttagctca
     3421 gttggtcaga gcgagcgact cataatcgcc aggtcgctgg ttcaagtcca gcaagggcca
     3481 ccaaccacca ctagctcatc cggatagagc atcaaccttc taagttgacg gtgcgaggtt
     3541 cgagtcctcg gtggtgggcc agcgccgact tagctcagca ggcagagcaa ctgacttgta
     3601 atcagtaggt caccagttcg attccggt
//
LOCUS       DAJTRW010000207         3612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRW010000207 DAJTRW010000000
VERSION     DAJTRW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..421)
                     /gene="ychE"
                     /locus_tag="OO326_004077"
     CDS             complement(<1..421)
                     /gene="ychE"
                     /locus_tag="OO326_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5968230.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVW"
     gene            898..3573
                     /gene="adhE"
                     /locus_tag="OO326_004078"
                     /gene_synonym="adhC"
     CDS             898..3573
                     /gene="adhE"
                     /locus_tag="OO326_004078"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5968231.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          966 a          904 c          855 g          887 t
ORIGIN      
        1 cccagacgat ggtagaactg atcgcccctg gccccgccat caacggcaac gccaatggca
       61 ccacaccaat actttcacgt accgcggttt ctgatttttc ttgcttgttc tgtttatcct
      121 cgccaagctt gccgctgatc atcgacatcg ctatcgtcac caccaggatg cccccggcga
      181 tacggaacga atcaattgat ataccaaaaa gttgtagaat cgtgtcgccg agaaaaagcg
      241 agatccacaa gataatggcc acagacaggt tggctgtaag gttagttttg tttcgcgctg
      301 ccgctgtctg ataactggtc atgctgataa agacgggaat aatccctacc gggttgacca
      361 gcgcaaataa cccgatgaaa aatttgaagt aaacgggaaa atcaaaaagg gtctgaatca
      421 cggttcgctc cgaagcaaaa gccggataat gttagccata aataaggttg aaaagacgcg
      481 ctgacaatac gccttttgac agcatttttc acctcctaac tacttaaaat tgctatcatt
      541 cgttattgtt atctagttgt gcaaaacatg ctaatgtagc caccaaatca tactacaatt
      601 tattaactgt tagctataat ggcgaaaagc gatgctgaaa ggtgtcagct ttgcaaaaat
      661 ttgatttgga tcacgtaatc agtacccaga agtgagtaat cttgcttacg ccacctggaa
      721 gtgacgcatt agagataata actctaatgt ttaaactctt ttagtaaatc acagtgagtg
      781 tgagcgcgag taagcttttg attttcatcg gttaagcaaa tcatcaccgc actgactata
      841 ctctcgtatt cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg
      901 gctgttacta atgtcgctga acttaacgca ctcgtagagc gtgtaaaaaa agcccagcgt
      961 gaatatgcca gtttcactca agagcaagta gacaaaatct tccgcgccgc cgctctggct
     1021 gctgcagatg ctcgaatccc actcgcgaaa atggccgttg ccgaatccgg catgggtatc
     1081 gtcgaagata aagtgatcaa aaaccacttt gcttctgaat atatctacaa cgcctataaa
     1141 gatgaaaaaa cctgtggtgt tctgtctgaa gacgacactt ttggtaccat cactatcgct
     1201 gaaccaatcg gtattatttg cggtatcgtt ccgaccacta acccgacttc aactgctatc
     1261 ttcaaatcgc tgatcagcct gaagacccgt aacgccatta tcttctcccc gcacccgcgt
     1321 gcaaaagatg caaccaacaa agcggctgat atcgttctgc aggctgctat cgctgccggt
     1381 gctccgaaag atctgatcgg ctggatcgat caaccttctg ttgaactgtc taacgcactg
     1441 atgcaccacc cagacatcaa cctgatcctc gcgactggtg gtccgggcat ggttaaagcc
     1501 gcatacagct ccggtaaacc agctatcggt gtaggcgcgg gtaacactcc agttgttatt
     1561 gatgaaactg ctgatatcaa acgtgcagtt gcatctgtac tgatgtccaa aaccttcgac
     1621 aacggcgtaa tctgtgcttc tgaacagtct gttgttgttg ttgactctgt ttatgacgca
     1681 gtacgtgaac gtttcgcaac ccacggcggc tatctgttgc agggtaaaga gctgaaagct
     1741 gttcaggatg ttatcctgaa aaatggtgcg ctgaacgcgg ctatcgttgg tcagccagcc
     1801 tataaaattg ctgaactggc aggcttctct gtaccagaaa acaccaagat tctgatcggt
     1861 gaagtgaccg ttgttgatga aagcgaaccg ttcgcacatg aaaaactgtc cccgactctg
     1921 gcaatgtacc gcgctaaaga tttcgaagac gcggtagaaa aagcagagaa actggttgct
     1981 atgggcggta tcggtcatac ctcttgcctg tacactgacc aggataacca accggctcgc
     2041 gtttcttact tcggtcagaa aatgaaaaca gctcgtatcc tgattaacac cccggcttct
     2101 cagggtggta tcggtgacct gtataacttc aaactcgcac cttccctgac tctgggttgt
     2161 ggttcctggg gtggtaactc catctctgaa aacgttggtc cgaaacacct gatcaacaag
     2221 aaaaccgttg ctaagcgagc tgaaaacatg ttgtggcaca aacttccgaa atctatctac
     2281 ttccgccgtg gctccctgcc aatcgcgctg gatgaagtga ttactgatgg ccacaaacgt
     2341 gcgctcatcg tgactgaccg cttcctgttc aacaatggtt atgctgatca gatcacttcc
     2401 gtactgaaag cagcaggcgt tgaaactgaa gtcttcttcg aagtagaagc ggacccgacc
     2461 ctgagcatcg ttcgtaaagg tgcagaactg gcaaactcct tcaaaccaga cgtgattatc
     2521 gcgctgggtg gtggttcccc gatggacgcc gcgaagatca tgtgggttat gtacgaacat
     2581 ccggaaactc acttcgaaga gctggcgctg cgctttatgg atatccgtaa acgtatctac
     2641 aagttcccga aaatgggcgt gaaagcgaaa atgatcgctg tcaccaccac ttctggtaca
     2701 ggttctgaag tcactccgtt tgcggttgta actgacgacg ctactggtca gaaatacccg
     2761 ctggcggact atgcgctgac cccggatatg gcgattgtcg acgccaacct ggttatggac
     2821 atgccgaagt ccctgtgtgc tttcggtggt ctggacgcag taactcacgc catggaagct
     2881 tatgtttctg tactggcatc tgagttctct gatggtcagg ctctgcaggc actgaaactg
     2941 ctgaaagaat atctgccagc gtcctaccac gaagggtcta aaaatccggt agcgcgtgaa
     3001 cgtgttcaca gtgcagcaac tatcgcgggt atcgcatttg cgaacgcctt cctgggtgta
     3061 tgtcactcaa tggcgcacaa actgggttcc cagttccata ttccgcacgg tctggcaaac
     3121 gccctgctga tttgtaacgt tattcgctac aatgcgaacg acaacccgac caagcagact
     3181 gcattcagcc agtatgaccg tccgcaggct cgccgtcgtt atgctgaaat tgccgaccac
     3241 ctgggtctga gcgcaccggg cgaccgtact gctgctaaga tcgagaaact gctggcatgg
     3301 ctggaaacgc tgaaagctga actgggtatt ccgaaatcta tccgtgaagc tggcgttcag
     3361 gaagcagact tcctggcgaa cgtggataaa ctgtctgaag atgcattcga tgaccagtgc
     3421 accggcgcta acccgcgtta cccgctgatc tccgagctga aacagattct gctggatacc
     3481 tactacggtc gtgattatgt agaagatgaa actgcagcga aaaaagaagc cgctccggct
     3541 aaagctgaga aaaaagcgaa aaaatccgct taatcagtag cgctgtctgg caatataaac
     3601 ggccccttct gg
//
LOCUS       DAJTRW010000208         3540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRW010000208 DAJTRW010000000
VERSION     DAJTRW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..455
                     /gene="pgrR"
                     /locus_tag="OO326_004079"
     CDS             <1..455
                     /gene="pgrR"
                     /locus_tag="OO326_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5968232.1"
                     /translation="EMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAI
                     NLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVER
                     AIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            792..2405
                     /gene="mppA"
                     /locus_tag="OO326_004080"
     CDS             792..2405
                     /gene="mppA"
                     /locus_tag="OO326_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5968233.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OO326_004081"
     CDS             complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OO326_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5968234.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          771 c          816 g          947 t
ORIGIN      
        1 gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg
       61 ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca caattaatag
      121 atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa
      181 tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag
      241 acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg
      301 ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc actccagatt
      361 tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat
      421 ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta
      481 attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt
      541 acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcccgaa
      601 cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt tacactaaat
      661 ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt ttttgatgga
      721 agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta
      781 gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg gtcagcagca
      841 tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc
      901 tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc
      961 cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac gaaaaagggg
     1021 agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc tggactttta
     1081 ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct
     1141 acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg tttgccgcgc
     1201 tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct gatcagcttg
     1261 gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt
     1321 ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg
     1381 gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc
     1441 gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat aacgccaaaa
     1501 cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt
     1561 acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt
     1621 tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat tattatgcct
     1681 ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta agtatgacga
     1741 tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca gcgtggcatt
     1801 ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc
     1861 aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa
     1921 aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg
     1981 ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat
     2041 ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg
     2101 tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg cattcaggaa
     2161 atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa
     2221 ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag
     2281 caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg tggctgaaag
     2341 gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat attgtgaagc
     2401 attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc
     2461 gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt
     2521 ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa
     2581 gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt
     2641 cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt
     2701 tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta
     2761 aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg
     2821 acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa
     2881 ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt
     2941 cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa
     3001 tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag
     3061 aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata atggtgattt
     3121 tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt
     3181 tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca atcagggacg
     3241 tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt tctataacat
     3301 aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat tgcaccgcat
     3361 ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc atcagaatca
     3421 atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag
     3481 cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggcgg
//
LOCUS       DAJTRW010000209         3441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRW010000209 DAJTRW010000000
VERSION     DAJTRW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..320
                     /locus_tag="OO326_004082"
     CDS             <1..320
                     /locus_tag="OO326_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5968235.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYA
                     SMA"
     gene            477..1226
                     /locus_tag="OO326_004083"
     CDS             477..1226
                     /locus_tag="OO326_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5968236.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1248..1814
                     /gene="yieF"
                     /locus_tag="OO326_004084"
                     /gene_synonym="chrR"
     CDS             1248..1814
                     /gene="yieF"
                     /locus_tag="OO326_004084"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5968237.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1868..3205)
                     /gene="adeP"
                     /locus_tag="OO326_004085"
     CDS             complement(1868..3205)
                     /gene="adeP"
                     /locus_tag="OO326_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5968238.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3370..>3441
                     /locus_tag="OO326_004086"
     CDS             3370..>3441
                     /locus_tag="OO326_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5968239.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSR"
BASE COUNT          946 a          902 c          849 g          744 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactcca tcaactgccg ttggttgctc
      181 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
      241 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
      301 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
      361 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
      421 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
      481 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
      541 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
      601 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
      661 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
      721 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
      781 atatggaact acagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
      841 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
      901 cgcagctcat tacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
      961 atccgcgcga tctgcagctg ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
     1021 atgtagaagc gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgcccg
     1081 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cagctggaaa agcctgccgg
     1141 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     1201 ccgtgaagtc tttttcgcca aattgataga caaccacagg agtcattatg tctgaaaaat
     1261 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     1321 gtaccctgcc gaaaattgct ccggcgagca tggaagtcaa tgcgttacct tccattgccg
     1381 acattcccct gtatgacgct gacgtacagc aggaagaagg ttttccagca acggttgaag
     1441 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     1501 actcggtacc gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     1561 cgctggccgg taaaccggtg ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc
     1621 gctgtcagta tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc
     1681 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     1741 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc
     1801 gagttaagat ctaaataaaa aacccgccag taatcatgca tggcgggttt ttaatgcgct
     1861 atcgatttta atgagcgtcg ataaatacaa tcttcaggat aaacagcagc gcaacgatga
     1921 ttacgcacgg gctaaggtca cgcagacgcc cggtaccaat cttcatcacg cagtaggaga
     1981 taaagcccag cgcaatacct tcggtaatcg aaaagctgaa cggcatcatc acggcggtaa
     2041 taaacgccgg aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca
     2101 tcaacacgcc aacgtagatc aacgcgccag ctgcagcgta gtctggcacc attcccgcca
     2161 gcggcgacag aaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg
     2221 tacgaccgcc aaccgatacg ccggaagagg actcaatata agccgtaacg gaagaagtac
     2281 cgataaacga accggtcacg gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg
     2341 ggaatttccc cttctcatcc gccagacctg ctttatcggt cacgccaatc agcgtaccgg
     2401 aggagtcaaa caagttgacc aacatgaaag agaaaatcac ccctgccagc ccgaggttaa
     2461 acgacccggc taaatctaca tgacccacaa ctgtcattac gctcggcggc gcagaaacga
     2521 tgccattgta gtgcacatca cccagcatcc agcccagcag cgtcgtcacc acgatagaaa
     2581 ccagcaccgc tgcgtgaatg ttgcgcgagg ccagaatagc aatgatgaaa aagccgagga
     2641 tacccagaag tacgctgtga gaagtcagat taccgatgct caccagcgtt tccgagttag
     2701 cgacaatcac acctgcgttt ttcagcccca tcatgccgat aaacaggccg ataccgctgg
     2761 taatacccac acgcagactt accggaatgt tagcaatcat ccagtaacga acgcggaaaa
     2821 tggtcaaaag aagcagtccg acagcgcccc agaagattgc gcccataccg atctgccacg
     2881 gcagccccat tgcctggaca accacaaaag cgaaaaaggc gttaagcccc atcgcaggag
     2941 ccagcgccac cggaaggttt gcaaacaatc ccatcataat gctaccgaaa gcagcaatca
     3001 gacaggttgt tacgaagacg gcactggtgt ccatgccagc aacgcccaga atttgtgggt
     3061 taacaaatac gatgtagacc atcgtcagga aggtggtgaa cccggcaatc acttcagttc
     3121 gtgccgtagt accatgttcg cgcagtttaa aaacacgttc cagcatcctc tgaccagaag
     3181 tctgggtggt atgttgatga ctcattatct atttccgaac aaggagggaa aatccgtcgc
     3241 tatcgtatac caaaatgcga caataggcgc gtttgtgaga gactttttta ttggatttac
     3301 ttatacggca acgattgcgt tgcgcaaatc ggctctacga aaacgttaaa ctgattaaaa
     3361 aggaaaggca tgtcccggat agaagcggta tttttcgact gcgacggtac gctggtcgac
     3421 agtgaagtca tttgctcccg c
//
LOCUS       DAJTRW010000210         3248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRW010000210 DAJTRW010000000
VERSION     DAJTRW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..442
                     /locus_tag="OO326_004087"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO326_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO326_004088"
     CDS             1026..1742
                     /locus_tag="OO326_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968240.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO326_004089"
     CDS             2294..2698
                     /locus_tag="OO326_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968241.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3248
                     /locus_tag="OO326_004090"
                     /pseudo
     CDS             2761..3248
                     /locus_tag="OO326_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          671 c          745 g          923 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgtccttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaa
//
LOCUS       DAJTRW010000211         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRW010000211 DAJTRW010000000
VERSION     DAJTRW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO326_004091"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO326_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5968242.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO326_004092"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO326_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTRW010000212         3114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRW010000212 DAJTRW010000000
VERSION     DAJTRW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO326_004093"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO326_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5968243.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO326_004094"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO326_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO326_004095"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO326_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5968244.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO326_004096"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO326_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5968245.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3114)
                     /locus_tag="OO326_004097"
                     /pseudo
     CDS             complement(2163..3114)
                     /locus_tag="OO326_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          754 c          758 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gggc
//
LOCUS       DAJTRW010000213         3107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRW010000213 DAJTRW010000000
VERSION     DAJTRW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..589
                     /locus_tag="OO326_004098"
     CDS             <1..589
                     /locus_tag="OO326_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5968246.1"
                     /translation="ISRIGRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCSDSSD
                     VDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADVPKNMTQQNCQSKYENCY
                     YDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRFGSG
                     KKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            592..1755
                     /locus_tag="OO326_004099"
     CDS             592..1755
                     /locus_tag="OO326_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5968247.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1839..>3107
                     /locus_tag="OO326_004100"
     CDS             1839..>3107
                     /locus_tag="OO326_004100"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5968248.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSI"
BASE COUNT          676 a          744 c          908 g          779 t
ORIGIN      
        1 gatcagtcgc attggtagac cgaataatcc ttttgaaccg cgtcgcaatc gctacgcaca
       61 aaaatactta acgttggcgc taatgggcgg tgccgctttt ttcgtattga aaggttgtag
      121 cgatagtagc gatgtcgata acgacggcga cggaacattt tacgcgacag tgcaggattg
      181 tattgatgac ggtaataatg ccgacatttg cgcgcgtggc tggaacaacg ccaaagcggc
      241 attttatgcc gatgttccaa agaatatgac tcagcagaac tgtcagtcta agtacgaaaa
      301 ttgctactac gacaatgttg aacagagttg gatcccggtc gtttctggat ttttgttaag
      361 ccgggttatt cgtaaagatc gcgatgagcc gttcgtctat aacagcggcg gatcctcatt
      421 tgcttcccgc cccgtctggc gcaacacttc cggtgattac tcctggcgct tcggttctgg
      481 caaaaaagag tcttactctt caggcggctt taccaccaga aaagcgtcga ccgtttcgcg
      541 cggcggctat ggtcgttctt ccagcgcccg tgggcattgg ggaggctaat catgctgaga
      601 cacaacgttc ctgtgcggcg ggatctggac cagatcgccg ctaacaacgg tttcgacttt
      661 catatcatcg acaatgaaat ctattgggac gagagtcggg cttaccgttt tactctgcgc
      721 cagattgaag agcagatcga aaaaccgact gcggaactgc atcagatgtg ccttgaggtg
      781 gtggatcgcg cggttaaaga cgaagagatc ctgacgcaac tggcgatccc gccgttgtac
      841 tgggatgtga tcgccgaaag ctggcgcgca cgcgatcctt cgctgtatgg ccggatggat
      901 tttgcctggt gtggcaatgc gccggtgaag ctgctggagt acaacgccga tacgccgacc
      961 tcgttgtatg agtcggctta tttccagtgg ttgtggctgg aggatgcccg gcgcagcggc
     1021 attattccgc gtgatgccga tcagtacaac gccattcagg aacgcctgat ttcgcgcttt
     1081 agtgagcttt acagtcggga actgttttat ttttgctgct gtcaggacac cgatgaagac
     1141 aggagtaccg tgctgtactt gcaggactgc gcccagcagg cagggcagga gtcgcggttt
     1201 atctacattg aagatctcgg tttgggcgtc ggcggcgtac tgaccgatct tgatgataat
     1261 gtcatccagc gtgcatttaa gctgtatccg ctggagtgga tgatgcgtga cgataacggt
     1321 ccgctgctgc gcaagcgtcg cgagcaatgg gtggagccgt tatggaaaag tatcttgagt
     1381 aataaagggc taatgccgct gctttggcgc ttcttccctg gtcatcctaa ccttcttgca
     1441 tcctggtttg agggtgaaaa atcgcagatt gccgctggcg aaagctatgt gcgtaaaccg
     1501 atctactcgc gcgaaggcgg aaacgtcacc attttcgacg gtcagaataa cgtcgttgac
     1561 cacgctgatg gtgattacgc cgatgaaccg atgatctacc aggcgtttca acctctgccg
     1621 cgatttggcg atagctacac actcatcggt agctggattg tcgatgatga agcgtgcgga
     1681 atggggatcc gtgaagataa cacactgatc accaaagaca cctcacgttt cgttccgcat
     1741 tacattgctg gataagaatg ttttagcaat ctctttctgt catgaatcca tggcagtgac
     1801 catactaatg gtgactgcca ttgatggagg gagacacagt gcactggcaa actcacacca
     1861 tttttaatca acctatacca ttaaataaca gcaacttata cctgtctgat ggcgcgcttt
     1921 gcgaagcggt aacgcgtgaa ggtgctggct gggatagcga ttttctagcc agtattggtc
     1981 agcagttagg aacggctgaa tcccttgaac tggggcggct ggcgaatgta aacccgcctg
     2041 aactgttgcg ctacgatgcc caggggcggc gtctggacga tgttcgtttc catcccgcat
     2101 ggcacctgct gatgcaggcg ttatgtacca atcgggtgca caatcttgcc tgggaagaag
     2161 acgctcgctc cggcgcattt gtggcgcgcg cggcgcgttt tatgttacat gcgcaggttg
     2221 aggctgggtc gttatgtcca gtaacgatga cctttgccgc cacgccattg ttgttacaga
     2281 tgttacccac gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc
     2341 acttattgcc aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc
     2401 agggcggttc cgatgtcatg agcaacacca cccgtgcaga gcgtttggaa gatggctctt
     2461 atcggctggt ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc
     2521 tggcgcagac cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc
     2581 aacgcaacgc gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca
     2641 gttgcgaagt ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa
     2701 ttcgtctgat cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg
     2761 ccatgatgcg ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg
     2821 gtaatccatt gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc
     2881 ttgaagggca aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg
     2941 ccaaagaagc cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac
     3001 gaggtatgcc gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg
     3061 agagcgagct gccgcggctt taccgggaga tgccggtaaa cagtatt
//
LOCUS       DAJTRW010000214         3101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRW010000214 DAJTRW010000000
VERSION     DAJTRW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            2..2905
                     /locus_tag="OO326_004101"
     rRNA            2..2905
                     /locus_tag="OO326_004101"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3101
                     /gene="rrf"
                     /locus_tag="OO326_004102"
     rRNA            3001..>3101
                     /gene="rrf"
                     /locus_tag="OO326_004102"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          817 a          684 c          970 g          630 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcgccg atggtagtgt ggggtctccc catgcgagag t
//
LOCUS       DAJTRW010000215         3099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRW010000215 DAJTRW010000000
VERSION     DAJTRW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(106..1815)
                     /locus_tag="OO326_004103"
     CDS             complement(106..1815)
                     /locus_tag="OO326_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5968249.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO326_004104"
     CDS             complement(2247..2966)
                     /locus_tag="OO326_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5968250.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DAJTRW010000216         3059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRW010000216 DAJTRW010000000
VERSION     DAJTRW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..488
                     /locus_tag="OO326_004105"
     CDS             <1..488
                     /locus_tag="OO326_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5968251.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            568..1263
                     /locus_tag="OO326_004106"
     CDS             568..1263
                     /locus_tag="OO326_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5968252.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1292..>3059
                     /locus_tag="OO326_004107"
     CDS             1292..>3059
                     /locus_tag="OO326_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5968253.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          885 a          717 c          679 g          778 t
ORIGIN      
        1 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
       61 actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt ccattcacta
      121 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      181 ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc cctaatctgg
      241 caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt attgacgatg
      301 cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg atttatcaga
      361 tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac gtatcaaccg
      421 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      481 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      541 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gatattaacc
      601 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      661 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca
      721 gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag ccgtgcccca
      781 tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc actgcgaatt
      841 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
      901 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
      961 aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt aaacgatgcg
     1021 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1081 tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag caaaattgaa
     1141 tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg aaagaacgtg
     1201 aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1261 taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc agggacataa
     1321 acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg tgctcactgg
     1381 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1441 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
     1501 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1561 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1621 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1681 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1741 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1801 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1861 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     1921 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     1981 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2041 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacgcctt
     2101 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2161 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2221 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2281 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt
     2341 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2401 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2461 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2521 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2581 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2641 tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag
     2701 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg
     2761 gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga
     2821 tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata acgagaaaaa
     2881 cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca gcctgaatta
     2941 tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga atctcagtat
     3001 tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat cacttaacc
//
LOCUS       DAJTRW010000217         3056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRW010000217 DAJTRW010000000
VERSION     DAJTRW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..198
                     /locus_tag="OO326_004108"
     CDS             <1..198
                     /locus_tag="OO326_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5968254.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="OO326_004109"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="OO326_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5968255.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="OO326_004110"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="OO326_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5968256.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="OO326_004111"
     CDS             1822..2577
                     /locus_tag="OO326_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5968257.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2978..>3056
                     /locus_tag="OO326_004112"
     CDS             2978..>3056
                     /locus_tag="OO326_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5968258.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGE"
BASE COUNT          796 a          683 c          725 g          852 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcgtattc tgactcggcc
     2761 cctggcgcag caaagtcagg catttatact ctttttaaca catagaaaat tcatatcaat
     2821 ataataaaaa cataaaatat ttgcatatac atataatctt aaagtttagt ctatttaatg
     2881 ttcaatgaaa tatttctacc tgtataatct ttaaagatgt tgaacatata ttcacattaa
     2941 atatgattat gtacttgtta caaggataag gttataaatg aataaagtta caaaaacagc
     3001 tattgtgggc ttacttgcgc ttttcgcagg taatgccgct gcaaccgatg gtgaaa
//
LOCUS       DAJTRW010000218         2834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRW010000218 DAJTRW010000000
VERSION     DAJTRW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..561
                     /gene="ssuE"
                     /locus_tag="OO326_004113"
     CDS             1..561
                     /gene="ssuE"
                     /locus_tag="OO326_004113"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5968259.1"
                     /translation="MAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAPEDLLY
                     ARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLAT
                     GGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTAL
                     ETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            554..1513
                     /gene="ssuA"
                     /locus_tag="OO326_004114"
     CDS             554..1513
                     /gene="ssuA"
                     /locus_tag="OO326_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5968260.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1510..2655
                     /gene="ssuD"
                     /locus_tag="OO326_004115"
     CDS             1510..2655
                     /gene="ssuD"
                     /locus_tag="OO326_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5968261.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2664..>2834
                     /locus_tag="OO326_004116"
     CDS             2664..>2834
                     /locus_tag="OO326_004116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5968262.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASG"
BASE COUNT          625 a          783 c          784 g          642 t
ORIGIN      
        1 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
       61 aaactaaatg gcctagatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
      121 gatctgcttt atgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      181 caggctgatg ggctgattgt cgccacgcct gtgtataaag ccgcctattc cggcgcgctg
      241 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      301 gcgacgggcg gtaccgtggc ccatctgctg gcggtcgatt atgcccttaa accagtttta
      361 agcgcactga aagctcagga gatcctgcac ggcgtgtttg ccgatgactc acaattaatt
      421 gattaccatc acagacccca gttcacgcca aatctgcaaa cccatcttga taccgcgcta
      481 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      541 cgaggtaatg cccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc
      601 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      661 cagtattggt atggtgctgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      721 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      781 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      841 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      901 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
      961 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaaattcac
     1021 cgatatccag cccacttacc tgacgcctgc cgatgcccgc gccgcgttcc agcaaggtaa
     1081 cgttgacgcc tgggctatct gggatcccta ctactccgct gcgttattac agggcggcgt
     1141 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1201 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctggcaacct ttagtgaggc
     1261 cgatgcgtta acccgcagcc agcgcgaaca aagcatcgct ttactggcaa aaacgatggg
     1321 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1381 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1441 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
     1501 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccacggtga cgggcactat
     1561 ctgggaacgg aagaaggttc acgcccggtt gatcacggtt atctgcaaca aattgcgcaa
     1621 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1681 gcgtggctgg ttgcagcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1741 ctgcgtccca gcgtaacctc acctactgtt gccgcccgcc aggccgccac gcttgaccgt
     1801 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca
     1861 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1921 gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg
     1981 cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc
     2041 ggatcgtcag atgtcgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg
     2101 ggcgaaccgc cggaacttgt taaagagaaa atcgaacaag tgcgggcgaa agctgccgcg
     2161 tatgggcgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac
     2221 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac tatcgccaaa
     2281 gcacaagccg cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgctacat
     2341 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2401 cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgccgc gcgaatcaac
     2461 gaatacgccg cgcttggcat cgacagcttt gtgctttcgg gctacccgca tctggaagaa
     2521 gcgtatcggg ttggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2581 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcaa atgattttat cccccgtaaa
     2641 gtcgcgcaaa gctaaggaac acgatgatgg caacgccagt gaagaagtgg ttattgcgcg
     2701 ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg gcaactggcc tcctcggttg
     2761 gctggctgtc gacgcgtatt ttgccttcac cggaaggagt ggtgacggcg ttctggacgc
     2821 tctccgccag cggc
//
LOCUS       DAJTRW010000219         2812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRW010000219 DAJTRW010000000
VERSION     DAJTRW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..779
                     /locus_tag="OO326_004117"
     CDS             <1..779
                     /locus_tag="OO326_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5968263.1"
                     /translation="VGCDRVEPGNVGIKVNKLGDDKGVGEVVGVGRYWTGWNTEVYIF
                     PTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTVFQTYRKGVDDITDTDLR
                     QKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTPIGIQVMSLSYVGKPEYP
                     PTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQADAIRTKAQAEADAIRL
                     RGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            823..1572
                     /locus_tag="OO326_004118"
     CDS             823..1572
                     /locus_tag="OO326_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5968264.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1569..2138
                     /locus_tag="OO326_004119"
     CDS             1569..2138
                     /locus_tag="OO326_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968265.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2262..2504
                     /locus_tag="OO326_004120"
     CDS             2262..2504
                     /locus_tag="OO326_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5968266.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2508..2654
                     /locus_tag="OO326_004121"
     CDS             2508..2654
                     /locus_tag="OO326_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968267.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2663..>2812
                     /gene="xisR"
                     /locus_tag="OO326_004122"
     CDS             2663..>2812
                     /gene="xisR"
                     /locus_tag="OO326_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5968268.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDG"
BASE COUNT          763 a          680 c          749 g          620 t
ORIGIN      
        1 ttgtcggttg cgatcgcgtt gagcctggta atgttggcat caaggtaaac aaactgggcg
       61 acgataaagg cgtcggagaa gtggtcggtg ttgggcgcta ctggacggga tggaacactg
      121 aggtttacat cttcccgacc ttcaaacaaa tgaagaccta cgatgagcca ttcagcttcc
      181 agatgagtga cggtacaacc atcggctatc acatcggcgt ggcctacaaa gttgatccat
      241 ccaaagttac cacagtcttt cagacctacc gcaaaggcgt ggacgacatt accgacaccg
      301 acctgcgcca gaagatagcc gacgcactca accgactggc cagcaaaatg accaccgaca
      361 aatttatcga cggcggcaag tctgagctgc tggatgcagc tcttaaagac attcaggaag
      421 aaatgacgcc catcggtatt caggtaatga gcctctcata tgtgggtaag ccggagtacc
      481 cgcctactgt tatcgacagc attaatgcca aagtcacggc gaaccagaaa accctgcaat
      541 gcgagcagga agtaaaacag cgcgaagcgg aagccaacat gttgcgcgcg gaagctgccg
      601 gacaggcaga tgcgattcgc acaaaagccc aggccgaagc cgatgctatt cgtttacgcg
      661 gtgaagctct gcgccagaac ccaaacgtta tggagctgga agccatcaac aagtggaacg
      721 gtacactgcc gcaatacatg accagcggtg ccaacacacc atttattcag gttaagtaat
      781 acatatgccc ggtattacac gccgggctgt ctggagataa aaatgaatac tgtaaccatc
      841 aacaacaaac agtttccggt aatcgaatat cgcggtcagc gtgttgtgac gctggcgatg
      901 attgatgaag tccaccagcg accggaagga accgcgcgtg cagcattcaa ccgaaaccgt
      961 gagcatttca tcagcggtgt ggattatgcc gaattaggtg cggacgtaat acgtacggac
     1021 ctcccggaag gaacattctc taaatttgca ccatcgggga ttgtactttt tgaatcaggt
     1081 tacctgatgt tgacgaagcc attcaatgac gcccttgcct ggcaggttca gcgcgaactg
     1141 gttaacagct acttccgcac tcgcacaccg ctaacggaaa tcgagatgat cgctgcgata
     1201 gccgccgacg ctgttcgtca acagaagcgc ctgaatcatg tggaagaaca gctcgaaaca
     1261 gtcacagaag cggtggaaac catcaaacgc ggaaatatgc gcgccggata tgtcggttac
     1321 cgccaggtgg tagccaaaag tggaatgagt gacaccaagt gtcggaatct ggtgaacgca
     1381 tatcaaattc ctacagacac ccacgaattc atgactccgg acggtctgtt gtctcgcagg
     1441 gctatcgtcg agcttgagcc atttatggcg gcgttccacc aaatgatgtc agaggctgac
     1501 ccacgcggca cacgctggta tcacccgaag atggggcttt tccaggtgat cggatgggag
     1561 gataaagcat gatcatccag tcaaaactta ttcgcgccgc tctggtgtgc gctgctaaaa
     1621 acgacgttcg ttattacctg aacggtcttc acatcacgcc gaaacatatt gaggcaacca
     1681 atggttccgt ggcactgcgc atggctcacg gcatccggac gaagaaaaac atcattgtcc
     1741 agttcgaagg gggcgtcccg gccaaagccg aaacgacaga gctgattttt agtaaagagc
     1801 cgatcgctgt tcatcgcgac cagtttcagc gacgactgtc cattaccggc attaaactgg
     1861 tggacggttg ttttccggat ttggatcgca tcattccgaa aaagtttgac cgctgtactc
     1921 acccggtgct ccaggcgggt tacctgagtt atccagagaa gatgtttggt cgtgagcgta
     1981 aatttattcc cgtccagtta cgtccctccg gtgacgggca agcggtcaga attcagtttg
     2041 attccatcat caactcaatg tatggcaatc ctgaatttgt tgtgatgcct tgtcgtgatc
     2101 atggcgattt caacgtggct caggagcatc cggaatgaaa atcgaatacc aggacgccgc
     2161 aggaggtgaa tcaatgagct ggcctgatgc aatcgtaact ctggggatgg tattcgcagt
     2221 agcgtttgtt gtgtactcga tttgtcgatg gggataacca catgttcgct ttgattcaac
     2281 gcgggcagat atacacagac agagccggat acccagtaga aattactcgc agtactgagc
     2341 actcagtgtt ctttcggagg atggacggac gtaccgggcg agtacgcatc ggtgaattca
     2401 gtagcctgtt cgagcatatt gaccacctgg aatatcacaa gattctggct gaaacagaac
     2461 aagaaaagca cctgaagaaa ttacgcgcaa tgcaaaggaa gtaaaaaatg aataaagcgt
     2521 ttgagctatg ggtccaccag cgttacggca accactacga cctgacgcga gatgttgacg
     2581 gtttctactg tcgtgaaatt gtgaaacgaa tgtttgaggt ctggtgccac tgccgtgggc
     2641 tgagtgttgt gtgaggtaat gcatgggcaa tgtgattcaa ctggctccca atgaatgggt
     2701 ttgtgaaagc gttctaatcg caattaccgg gctcaaacca ggcacaattc ttcggacccg
     2761 gaaagaatgc tggatggttg gaagagagta tattcacgta tcaccagacg gt
//
LOCUS       DAJTRW010000220         2734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRW010000220 DAJTRW010000000
VERSION     DAJTRW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            106..852
                     /locus_tag="OO326_004123"
                     /pseudo
     CDS             106..852
                     /locus_tag="OO326_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            867..1289
                     /locus_tag="OO326_004124"
     CDS             867..1289
                     /locus_tag="OO326_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5968269.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1347..1703
                     /locus_tag="OO326_004125"
     CDS             1347..1703
                     /locus_tag="OO326_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968270.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            1872..2537
                     /locus_tag="OO326_004126"
     CDS             1872..2537
                     /locus_tag="OO326_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5968271.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          735 a          608 c          757 g          634 t
ORIGIN      
        1 ctgttcgcgc cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg
       61 ttgccggaca gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg
      121 acaggcggcg ttctggaacg catccgccga ctgaccccgc aacatgtaac cgccccattc
      181 agaacggtag cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag
      241 atcaaccgcc tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag
      301 ccgttgcacc gtaaatgctc gtttgcgaat taccaggtgc agaacgacgg ccagcgatac
      361 gcgttaagcc aggcgaaatc catcgccgat gaactgatga ccgggtgtac aaattttgcg
      421 ttcagcggaa aacctgatac cgggtagaat cacttagcgg cagctatcgg gaatcgcctg
      481 ctgaaagacg gtcagacagt gattgtggtt accgtggctg atgttatgag cgccctgcac
      541 gccagctatg acgacgggca gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat
      601 ctgctggttc ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtgctg
      661 caccagattg ttgatcgccg gacagcgtcg atgcgcagcg tggggatgct gacaaacctg
      721 aactatgagg tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac
      781 ggcgggcgct gggttaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
      841 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
      901 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
      961 tgtccgatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
     1021 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
     1081 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
     1141 atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga
     1201 atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
     1261 agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc
     1321 tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata
     1381 tcgttttatt tccgataaaa gaggatgacc ctcgtaatca ggttaatttt ctttataagc
     1441 catcggaaag accatattgc catcacgcct ctgtacgggt tgacgaaaaa gagcgtcagg
     1501 tccgctgtaa aatctgcggt gcggttgtgg agccgtttga ctggatgctc tctgtggcaa
     1561 aaagagaaac cagactggca gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc
     1621 ggagaaatat agaaaagctg atacagattg agcgtaacgc gaaagcgcgg atacgcaggg
     1681 cgacaaaatc cagaactgaa taattaaatt tagcactgtt aaaaatttaa tccttaaccg
     1741 gagggatttc tgcaccctca aatcatcagg agaccgcccg aaagggcggg gagcagtcac
     1801 acatctgttt ccgatagccc cgttctaatg ctacactctt tgatattttt atgacctcaa
     1861 taaacatatt tatgacagtt gctgatttca aacggcccaa attggagctc ccaaacgggg
     1921 caaacaaact actactgcac tcttgctgtg ctccatgttc cggtgaggtg atggaggcgc
     1981 ttcaggcctc ggaaatcgac tacaccatct ttttctacaa cccgaacatt catcctcaga
     2041 aagagtattt aattcgtaag gatgagaata ttcgctttgc tgaacaacac ggcgtgccgt
     2101 ttattgatgc tgattacgac acagacaact ggtttgaacg agccaaagga atggaatggg
     2161 agcccgaacg agggatccgt tgcaccatgt gttttgacat gcgttttgag cggacagcgc
     2221 tgtacgccgc tgaaaatggt ttcagtgtga tcagcagttc actgggcatt tcacgctgga
     2281 aaaatatgca gcaggttaac gactgtgggc ggcgagccgt cgcgcattat ccgggcatgg
     2341 tgtactggga ttataactgg cgcaagcagg gcggctcgtc ccgtatgatt gaaatcagca
     2401 agcgcgaaaa attctatcag caggaatatt gtggctgtgt gtattctctg cgcgatacca
     2461 atctacaccg caaatctcag ggacgccctc ttatcaaaat tggtcaactc cactacggta
     2521 aagaagagaa ggagtgattt tatggggcac ctttctgatt gatttcatat tggcgaggta
     2581 agtagaatga ctgcgggtgc ttgaggctat ctgcttcagg catgaacacc aaaaggcaga
     2641 tagagaaaag ccccagttaa cattacgcgt ccggcaagac gcttaacatt aatctgaggc
     2701 caatttcatg ctttgcacat gtaggttagc ctct
//
LOCUS       DAJTRW010000221         2687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRW010000221 DAJTRW010000000
VERSION     DAJTRW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..588
                     /gene="lsrF"
                     /locus_tag="OO326_004127"
     CDS             <1..588
                     /gene="lsrF"
                     /locus_tag="OO326_004127"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5968272.1"
                     /translation="PPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAA
                     QVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMG
                     AQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRNI
                     FQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            612..902
                     /gene="lsrG"
                     /locus_tag="OO326_004128"
     CDS             612..902
                     /gene="lsrG"
                     /locus_tag="OO326_004128"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5968273.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            942..1700
                     /gene="tam"
                     /locus_tag="OO326_004129"
     CDS             942..1700
                     /gene="tam"
                     /locus_tag="OO326_004129"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5968274.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1704..2618)
                     /locus_tag="OO326_004130"
     CDS             complement(1704..2618)
                     /locus_tag="OO326_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5968275.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          709 a          626 c          721 g          631 t
ORIGIN      
        1 cccccggcga ccaataagcc ggtggtactg cgggcgtcag gtgcgaactc tattctggcg
       61 gaattaagta atgaagccgt ggcgttatcg atggatgatg ccgtgcgcct gaacagttgc
      121 gcggtggcgg cgcaggttta tatcggcagc gaatatgaac atcagtcgat caaaaatatt
      181 attcagctgg tcgatgccgg aatgaaagtg ggaatgccga ccatggccgt gactggcgtg
      241 ggcaaagata tggtgcgcga tcagcgttat ttctcgctcg cgactcgaat cgccgctgaa
      301 atgggggcgc aaattatcaa aacctattat gtcgaaaaag gttttgaacg gattgttgcc
      361 ggatgtccgg tacccattgt tattgctggc ggtaaaaaat taccggagcg cgagacgctg
      421 gaaatgtgct ggcaggctat cgatcagggc gcttctggtg tggatatggg gcgtaatatt
      481 ttccagtctg accatccggt ggcgatgatg aaagccgtac aggcggtggt tcaccataac
      541 gaaacggctg atcgggcata tgaactctat ctgagtgaaa aacagtaact gcggatttaa
      601 ggagaagaat tatgcacgtc acactggttg aaattaacgt tcatgaagac aaggttgacg
      661 agtttatcga agtttttcgc cagaaccacc tgggctctgt acaggaagaa ggtaatttgc
      721 gcttcgatgt cttacaggac ccggaagtga attcgcgctt ttatatttac gaagcctata
      781 aagatgaaga cacagtggca ttccataaaa ccacgcctca ctacaaaacc tgtgtcgcga
      841 aactggaatc tttaatgacc gggccgcgta aaaaacgtct gttcaatggg ttgatgccgt
      901 gaggctacaa ttggggtaac gcgctgacgg gagtaaaaaa aatgtctgac tggaacccct
      961 ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg cttgccagag
     1021 tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt aacagcaccg
     1081 ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg tctccggcga
     1141 tgattgctga agcgcgcagt gctttgccag actgccagtt cgtggaagca gacatccgca
     1201 actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg caatggctgc
     1261 ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag ggcgtgctgg
     1321 cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcgc gaagttgctt
     1381 gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat gcttactacg
     1441 atattttgag cgaagccgga tgtgaggtcg atatctggcg aaccacttac tatcaccaga
     1501 tgccgtcgca ccaggcgatt atcgattggg tgaccgccac tggattacgt ccgtggttac
     1561 aggatctgac cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag atgctggaag
     1621 agcagtatcc actgcaagag aacggacaga tactgctggc atttccgcgt ctgtttattg
     1681 ttgcccgccg tacggagtaa ttatcacgtc agctggtaat gacgatcggg aagaattttt
     1741 gctggaattt ctgcttcatc gttcatctgt aacaggtcaa tttcaatagc gttgcagatg
     1801 gcatccagcg gtaaatcatt gttttcagta ccgaacggat cttccagttc ttccgccaga
     1861 caatccagcg aaataaaagt gtaggaaatc agcacagaga taaaaggcgt catgtaatgc
     1921 aggtccacga ccagcgcgaa cggcagcatg atacaaaaca gataaacggt acgatgcaaa
     1981 atcagggtgt aggcaaaggg aattggcgtg taggcaatgc gctcacagcc tgccagaacc
     2041 gctgaaatat cattaagccg atcgttgagg ctaataaaca ggatatctga aagctgtcca
     2101 ttgcggcgct gaaccgccaa ccattctccc attattaaca agatacggtt agctggagag
     2161 ttcgaagcca gtacacgctg aagatctcca gtcttgagat aatgagccag cacttccgcc
     2221 tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg cagcctggca
     2281 aactccctta cacttgccga atccggcaat gtcgttttta cctctcgcag taacgaccgt
     2341 gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc gtacccggca
     2401 ttattacgaa aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa cggtgcgagg
     2461 gtgaatttga tgcccagatg cgtgtaccag ggcagcatga atataacagc aatagaaaag
     2521 agaaaattga gtagtaagcg cgaggatatc ttggataata ctgagccgtg ccagacaaaa
     2581 atacggcgca gccagtgttg ttgtggacga acaatcatgg ttatcttcag gcgtggaaaa
     2641 gtcgccctat taaacgtgat tacgatcaca ttctcaagac gctactt
//
LOCUS       DAJTRW010000222         2656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRW010000222 DAJTRW010000000
VERSION     DAJTRW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..567
                     /locus_tag="OO326_004131"
     CDS             <1..567
                     /locus_tag="OO326_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5968276.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="OO326_004132"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="OO326_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5968277.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO326_004133"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO326_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5968278.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="OO326_004134"
     CDS             2169..2405
                     /locus_tag="OO326_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5968279.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2444..2530)
                     /locus_tag="OO326_004135"
     tRNA            complement(2444..2530)
                     /locus_tag="OO326_004135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2494..2496),aa:Leu,seq:cag)
     gene            complement(2565..2651)
                     /locus_tag="OO326_004136"
     tRNA            complement(2565..2651)
                     /locus_tag="OO326_004136"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Leu,seq:cag)
BASE COUNT          675 a          602 c          679 g          700 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     2521 ccaccttcgc acagtcatct tacttttttt gatatcgcct cgtttggtgc gagggggggg
     2581 acttgaaccc ccacgtccgt aagaacacta acacctgaag ctagcgcgtc taccaattcc
     2641 gccaccttcg cccagt
//
LOCUS       DAJTRW010000223         2628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRW010000223 DAJTRW010000000
VERSION     DAJTRW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..545)
                     /locus_tag="OO326_004137"
     CDS             complement(<1..545)
                     /locus_tag="OO326_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968280.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO326_004138"
     CDS             complement(1207..2196)
                     /locus_tag="OO326_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968281.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2628)
                     /locus_tag="OO326_004139"
     CDS             complement(2280..>2628)
                     /locus_tag="OO326_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968282.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          681 a          614 c          632 g          701 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa ccgtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggtg
//
LOCUS       DAJTRW010000224         2585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRW010000224 DAJTRW010000000
VERSION     DAJTRW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            133..562
                     /locus_tag="OO326_004140"
                     /pseudo
     CDS             133..562
                     /locus_tag="OO326_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            749..986
                     /locus_tag="OO326_004141"
                     /pseudo
     CDS             749..986
                     /locus_tag="OO326_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1407..1985
                     /locus_tag="OO326_004142"
     CDS             1407..1985
                     /locus_tag="OO326_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968283.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2094..2352)
                     /locus_tag="OO326_004143"
                     /pseudo
     CDS             complement(2094..2352)
                     /locus_tag="OO326_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2336..2585
                     /locus_tag="OO326_004144"
                     /pseudo
     CDS             2336..2585
                     /locus_tag="OO326_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          781 a          476 c          668 g          660 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtgt ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat
      781 actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc
      841 aacagcgatc ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa
      901 aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa
      961 tggtggaaaa cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc
     1021 atcccctaaa cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt
     1081 atatctggcg aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa
     1141 tggaatggca gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga
     1201 acctgacgtg ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat
     1261 ggcatcctca tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca
     1321 gtaatgggga aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc
     1381 ctgttaatat ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt
     1441 ttcatgatat atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag
     1501 cagttttatc aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg
     1561 ccgaaaaaga caatgagata acaaagaaag acaaagccaa gataatagat acattaactg
     1621 aaaactattc cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat
     1681 ctttatatat tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa
     1741 tatgtcatac tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg
     1801 gtagcagcat tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg
     1861 agtctactgg cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat
     1921 ttatgaatga aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg
     1981 tttaatttta gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt
     2041 cagcagggcc tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg
     2101 tggtggttga ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag
     2161 aagcatatgg ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc
     2221 tctatccgct gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg
     2281 tataccggct caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag
     2341 agcagcgcgc atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt
     2401 ggcgaaacaa ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc
     2461 tgttgaaagt cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg
     2521 gatgaccgaa tccaggcagg aaaagctgat atggctgcgt gcgcagatga agctgacgag
     2581 cctgt
//
LOCUS       DAJTRW010000225         2562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRW010000225 DAJTRW010000000
VERSION     DAJTRW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(216..419)
                     /locus_tag="OO326_004145"
     CDS             complement(216..419)
                     /locus_tag="OO326_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968284.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(421..576)
                     /locus_tag="OO326_004146"
     CDS             complement(421..576)
                     /locus_tag="OO326_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5968285.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(779..1186)
                     /locus_tag="OO326_004147"
     CDS             complement(779..1186)
                     /locus_tag="OO326_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968286.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1263..1490
                     /locus_tag="OO326_004148"
     CDS             1263..1490
                     /locus_tag="OO326_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5968287.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1474..1896
                     /locus_tag="OO326_004149"
     CDS             1474..1896
                     /locus_tag="OO326_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968288.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1974..>2562
                     /locus_tag="OO326_004150"
     CDS             1974..>2562
                     /locus_tag="OO326_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968289.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTE"
BASE COUNT          663 a          547 c          659 g          693 t
ORIGIN      
        1 acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc ccccatggag
       61 atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc ggtgatattt
      121 atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat cgcgcgtctg
      181 tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag acccggtgaa
      241 tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc gccagattcc
      301 agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc cagttgctgg
      361 aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt ctcgttcatt
      421 ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt taaacgtttt
      481 gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca cgccgtacac
      541 cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc cccttaacgc
      601 cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat aaaatttaga
      661 ataacctaat gagggtgatc aagatttttg tgtagaaaaa cctaagtttt ttgatgtaaa
      721 aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa aaaggggatt
      781 atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg ttgaaatttt
      841 caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc agtgcgtcga
      901 acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta tccgggatcg
      961 gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag cactgaagcg
     1021 cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt tcccattgag
     1081 aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac agttttaatt
     1141 ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta gggaattcta
     1201 atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct aaggagctcg
     1261 atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt gccaatgccg
     1321 caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa ggtcgcgcga
     1381 tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt tatgacgaat
     1441 atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga gcacatcgaa
     1501 tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa cgcaattact
     1561 gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa ggactggaac
     1621 aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca aagggaaaaa
     1681 attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg tcaccgactc
     1741 tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc gttgagtacg
     1801 gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca tttcagcggt
     1861 ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt gccggattgt
     1921 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
     1981 caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc aggcttgctg
     2041 agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc tgggcatggg
     2101 cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt acaaaaagtg
     2161 caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag gttggatggc
     2221 tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac ggaaaaagct
     2281 ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg aaacgaaaag
     2341 gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa gaggaagagg
     2401 aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     2461 tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg catgagtggg
     2521 tccggttcag acaggcattg cgaaaaccga ttcgaacgga gc
//
LOCUS       DAJTRW010000226         2520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRW010000226 DAJTRW010000000
VERSION     DAJTRW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            6..224
                     /locus_tag="OO326_004151"
     CDS             6..224
                     /locus_tag="OO326_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5968290.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO326_004152"
     CDS             226..591
                     /locus_tag="OO326_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5968291.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO326_004153"
     CDS             588..1253
                     /locus_tag="OO326_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968292.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO326_004154"
     CDS             1253..1618
                     /locus_tag="OO326_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5968293.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="OO326_004155"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="OO326_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5968294.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          708 a          524 c          655 g          633 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gacccggcgg gggagaaatc cctcgccacc gctgatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
//
LOCUS       DAJTRW010000227         2510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRW010000227 DAJTRW010000000
VERSION     DAJTRW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(64..822)
                     /locus_tag="OO326_004156"
     CDS             complement(64..822)
                     /locus_tag="OO326_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5968295.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO326_004157"
     CDS             complement(830..1933)
                     /locus_tag="OO326_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5968296.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2510)
                     /locus_tag="OO326_004158"
     CDS             complement(1943..>2510)
                     /locus_tag="OO326_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5968297.1"
                     /translation="YQIKTGQLRRSWPIAAVLIIGLPLLAQWLFGAALHWDVPALQGF
                     NFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQHEAARSLGLPNPVTLR
                     QVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSLFAGTVLNQTGQAIETIAM
                     TMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          684 a          671 c          589 g          566 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat
//
LOCUS       DAJTRW010000228         2475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRW010000228 DAJTRW010000000
VERSION     DAJTRW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(6..725)
                     /gene="yehT"
                     /locus_tag="OO326_004159"
     CDS             complement(6..725)
                     /gene="yehT"
                     /locus_tag="OO326_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5968298.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(722..2407)
                     /gene="btsS"
                     /locus_tag="OO326_004160"
     CDS             complement(722..2407)
                     /gene="btsS"
                     /locus_tag="OO326_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5968299.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          631 a          674 c          565 g          605 t
ORIGIN      
        1 gtcttttaca ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg
       61 gttaagccat tacgcaaaat caactcggcc tggccgttat cttccagacg aatctcctgt
      121 aaatgcgcga ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc
      181 aaggtacgta atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca
      241 ccgctcatcc gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta
      301 tgccccgtac aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc
      361 tgcttgctgc gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt
      421 ggcttcagca gataatcaaa ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac
      481 gcagtgagaa aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga
      541 ccactgatgc gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc
      601 gcgccgatcc cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc
      661 tgcaaaaata tacgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta
      721 atcatgcctc gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac
      781 aggcgacgct tattccatag tcatcgccaa accgttcacg taaacgctta tccaccagat
      841 tcatccccag cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct
      901 ccagcatcaa atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca
      961 gttgtgatgt cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca
     1021 attgctgctg ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc
     1081 gcgccttttc aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa
     1141 actccgaagg ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc
     1201 tggcctgttc gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa
     1261 aatgaggatt cacctgggcg tgaagcagtt tgatttctga ctgggtgagc atcgcttttt
     1321 gccgctcata ttgccctgca agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca
     1381 gcgtgcggtt tattgaactg aataaccggt ttttggcttc atacaatttg atggtgccca
     1441 tcacgcgctg attttcgcca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg
     1501 gatgcaaaga gcaacgataa ggtacttcgt tgccatcagc gtagaccact tcaccggttt
     1561 caatcgcttt taaggtgtaa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac
     1621 caattccggt aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca
     1681 gctcctgata cagtacctga gccactttca tgctgttcac ttcgttaaac ccctgtcgca
     1741 aaatgccttc cgtcgaggct gccactttca gcgcagtggc agaaaaagcc gaagtgtatt
     1801 tttcaaacat cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat
     1861 tggtgaccat cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc
     1921 gggcgatcgc aaggatgatc agcatttgca ccatttcagc gacgaacgtg acggcaccgg
     1981 cggtaatggg gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca
     2041 ggccgccgag caatccttca acgatggttg agatcatgca acttagcgcg gtcatgcccc
     2101 ccatcgaata tcgatgtaag ccgccggtca gaccaaccag cccaccgacg accggaccgc
     2161 cgagtaagcc gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt
     2221 gcaacccaaa ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt
     2281 tatgcggcag acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta
     2341 accacgcaat gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat
     2401 cgtacatacc cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta
     2461 actttctttg aactc
//
LOCUS       DAJTRW010000229         2447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRW010000229 DAJTRW010000000
VERSION     DAJTRW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..226
                     /locus_tag="OO326_004161"
     CDS             <1..226
                     /locus_tag="OO326_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5968300.1"
                     /translation="ENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPE
                     DWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(480..644)
                     /locus_tag="OO326_004162"
     CDS             complement(480..644)
                     /locus_tag="OO326_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968301.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1546..1830
                     /locus_tag="OO326_004163"
     CDS             1546..1830
                     /locus_tag="OO326_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5968302.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1818..2303
                     /locus_tag="OO326_004164"
     CDS             1818..2303
                     /locus_tag="OO326_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5968303.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          633 a          522 c          693 g          599 t
ORIGIN      
        1 tgaaaacgcg ttacgatcgg tggcggttgg aagaaaaaat tatctctttt tcggctcaga
       61 caagggagga gaaagtgcgg cgatcatcta cagtctgctg gtcacctgca aacagaacga
      121 agtggagccg gaggactggt tgcgcgaagt gatcgagaag ctcaatgact ggccgtcgaa
      181 ccaagtgcat gaactgctgc cctggaactt ctcgtctgta aaataatcct tacgctacgt
      241 acttctcggg gcgcttacct tcaaaatgat ggacattatt tttgtggaac cggaggtaac
      301 agaccagacg gtttaaatga gccggttact ttaaacgggt gatggacagc acctcaaaag
      361 ttcagatgga ttatatttgt ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg
      421 aacgacttgc tcaggggata gctgatggag ttactgaagt accgaaggac cactaaaaat
      481 cacaggccgg gaatttcggc gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt
      541 gatgttggat gcaatgctgt atgagagatg aggggcggcg tccagacgca gccttcaaac
      601 cgccttgagc tgctgccgtt aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga
      661 acatgtgtca ggtaggtgtt gcggagatga acgaaagtga actgtcgatg aagtgtcgaa
      721 aggaacctct aggcgatgtc gaaaatgtgc tatctacgca gcgcatgacg agtccagcag
      781 atatctggta ctggctgggc ggcatccggc atataggcag catgaacgca acacaggctc
      841 tcgtacggaa cgtgggaacc tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca
      901 ctgcaagcgc ctgagtaccg atgcgagaca caggggcaga tggcttcgta gtagtgagga
      961 agtgcctgta atgggcatgg agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa
     1021 ccggatagcc gggaggagcc catgagtcag ccaaagccgt tcgaggtctc taagtatgcg
     1081 gtttggaaag cataccagcg ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag
     1141 tcagtcgagg cgtttgaggt taccaatcga agctataccc ggtaatggcg caggttgacc
     1201 tgcacttggc caagtggatt gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg
     1261 cattcgagtg gttactgcga attcgtcacg actgcccgac gctgtttgca cactggtgct
     1321 tagcttacga tacttgaatg gttcaacgac aagagccgta tgacgcgaga gtgtcacgta
     1381 cggctccgtg ggagcgtagg ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg
     1441 cggttacgtc cacagaggta aatgccgctg acgcggcatc tgtaatctgt tactttgtgt
     1501 atatcatttg ctatatattg tcaatatgct attgttgtaa agcaaatgat atacggaggc
     1561 tttatgaaat caggtgttca gctcaatctc agagcaagag aatcacagcg gatactcatt
     1621 gatgctgctg cagaaattct tcataagtcg cgtacagatt tcattctgga aatggcctgt
     1681 aaagctgctg aagatgttat tctcgaccgg cgggtgttta attttaatga caggcaatat
     1741 gaagagttca ttgaaatgct tgatgctccg gttgcagatg atcctgccat cgaaaaattg
     1801 ctggcaagga aacctcaatg ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc
     1861 aggtcgctga atttgtcagc agtgaagctg tacttgatga ctggttaaag cagaaggagc
     1921 taaaaaatca ggcaatcgga gccacccgga cttttgttgt atgcagaaaa ggtacgcaac
     1981 aaatagtcgg tttttactca ctggccactg gtagtgtcaa tcatacagaa gctacaggta
     2041 accttcggcg caatatgcca gaccccattc ctgtaattat acttgcacgc ctggctgtag
     2101 atgtgtcatt tcgaggaaaa gggcttggcg cggatttact tcatgatgca gttcgtcgtt
     2161 gttacagggt tgccgaaaat atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa
     2221 acgcaaaaca gttttatatt catcatggtt ttaagccatc aaaaactcag gtgcaaacat
     2281 tgtttcttaa actccctcaa tagggagtaa tgccgatcag ttaaggatca gttgaccgat
     2341 ccagtggctg tgtaagaatc cggaaacgct cactcgtttc cggatttttc tgtgcacagt
     2401 aaaagaccac ctcctgagga ggtggctttt cagaatgccc cctataa
//
LOCUS       DAJTRW010000230         2405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRW010000230 DAJTRW010000000
VERSION     DAJTRW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>2405
                     /locus_tag="OO326_004165"
     CDS             <1..>2405
                     /locus_tag="OO326_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5968304.1"
                     /translation="NERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYT
                     SYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAK
                     WRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDI
                     DSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPM
                     VQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFR
                     VNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRML
                     TSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNE
                     LAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTS
                     LTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELS
                     SSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWAS
                     LQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLF
                     SSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQN
                     EPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVD
                     PQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSLDIQQ
                     RSV"
BASE COUNT          697 a          562 c          584 g          562 t
ORIGIN      
        1 acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc acaaaaacat
       61 ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg
      121 ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg actacgcaga
      181 aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc gcaacctttg
      241 aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt gcccatcagg
      301 atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat acccgtaatt
      361 tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg gcacaattgc
      421 gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg gtcggcgata
      481 tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt ccggctaaca
      541 aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc tttaatatca
      601 ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg ccaatggtac
      661 aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta gttcagctgt
      721 tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt tctggcggca
      781 ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt gtaaatgcac
      841 gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca accattgatc
      901 agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc acctggctga
      961 aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt cgcctggcat
     1021 ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac
     1081 gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga
     1141 accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa aaagcattgt
     1201 ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg gcggcttaca
     1261 tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg gaaattagca
     1321 gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt gccaaggtta
     1381 ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta tcgaataaag
     1441 gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat aaagcagtta
     1501 gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt gcggaaaact
     1561 cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga
     1621 ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac agcacgatta
     1681 acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc
     1741 agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct gatgaccgca
     1801 cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg ctggctgccg
     1861 atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt aatgtcgcag
     1921 aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac tctgattcgc
     1981 cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag
     2041 aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc cggacacctg
     2101 ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca aaagatctac
     2161 gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc ctctcggttg
     2221 atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca gaacgacatg
     2281 atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag gggatcagtg
     2341 agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc caacagcgta
     2401 gcgtt
//
LOCUS       DAJTRW010000231         2404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRW010000231 DAJTRW010000000
VERSION     DAJTRW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..2264
                     /locus_tag="OO326_004166"
     CDS             <1..2264
                     /locus_tag="OO326_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5968305.1"
                     /translation="CFIAIKKTLSSFGIKVDALKSFNDVDETVCLDPAPRIEGSSWQF
                     DSDKLQLNISIHQIYMDAMAYDYISPTRWDEGINALTINYDFSGSHTLRSDYGSQETD
                     TSYLNLRNGLNIGPWRLRNYSTLNTSDGRAEYNSISTWIQRDIAALRSQIMIGDTWTA
                     SDIFDSTQIRGARLYTDNDMLPASQNGFAPVVRGIAKSNATVIIRQNGYVIYQSAVPQ
                     GAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLAILKREGLTDVDVSVGELRD
                     EDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHAR
                     ANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFW
                     ETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNV
                     SWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSGWWGNSYATYSVSQNDNSGSSHQ
                     LGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNY
                     GIRGGILAHSEGVTLSQELGETIALVKAPGAARLEIDNMRGAATDWRGYTVKTQLNPY
                     DENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPV
                     PFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSFSTPE
                     SESGLIEQGVTCH"
     gene            2275..>2404
                     /locus_tag="OO326_004167"
     CDS             2275..>2404
                     /locus_tag="OO326_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5968306.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          642 a          522 c          599 g          641 t
ORIGIN      
        1 cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt gatgcgctca
       61 aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt attgaaggct
      121 catcctggca gtttgacagt gataaattgc agctgaatat atccattcac caaatctaca
      181 tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg attaatgcgc
      241 tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat ggttcacaag
      301 agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg tggcggctac
      361 gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc attagtacct
      421 ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat acgtggacgg
      481 cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact gataacgata
      541 tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca aagtccaacg
      601 ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt ccacaaggtg
      661 cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac gtaaccatca
      721 aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg gcgattctta
      781 aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat gaagacggat
      841 ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg atcactttat
      901 atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc ggtaaagatc
      961 ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat tttagccatg
     1021 atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt gacgacacag
     1081 acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat accctcaatg
     1141 agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac cgacgtagtc
     1201 gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta tacctgacat
     1261 taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa tttggctaca
     1321 gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat actgccagac
     1381 aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac atgctctctt
     1441 tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct gtttcgcaaa
     1501 acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg gaaagaaata
     1561 acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt ggcggtaata
     1621 tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac tacagccaaa
     1681 attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc gaaggggtaa
     1741 cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg gccgccaggt
     1801 tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg gtcaagacac
     1861 agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc tcgaagtcga
     1921 atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca gtggttaaag
     1981 cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat gcaaatggta
     2041 aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca gattcaggaa
     2101 ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga caggttacgt
     2161 tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt tcgacaccag
     2221 aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat ttttatgaac
     2281 aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag tgtagcactt
     2341 gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac gcttgatttc
     2401 tcgg
//
LOCUS       DAJTRW010000232         2341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRW010000232 DAJTRW010000000
VERSION     DAJTRW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(624..2228)
                     /locus_tag="OO326_004168"
     CDS             complement(624..2228)
                     /locus_tag="OO326_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5968307.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          610 a          558 c          512 g          661 t
ORIGIN      
        1 aattgtgtct ttaagttaac tttaagtaat actataagtt aatctttaat tgtgtcttta
       61 agttaacttt aagttaagcc cctgtaggga ctagagacca taattaagat gattgtgtct
      121 tattgctatt cacttatcga ctgacccaga gcacacagga agcattcact ttaagtagaa
      181 cgcttggtgt ctgtaattgg tcatcccgcc cctgaaagga tgcgataagt tacggttagt
      241 tgtcctagaa gagaaaactt tttgtgtccc ttctccctat agtgagtcgt attaattatc
      301 cttgacaagt agagaaaacc atgagtcaaa aacatgacca cacaggtctg tagtaagtgt
      361 catcctagaa gagaaaactt tttgtgtccc ttctccctat agtgagtcgt attaattacc
      421 ccttgacaag cagagaaaac tatgagtcaa aaacatgacc gcacaggtct gtagtaagtg
      481 tcatcctaga agagaaaact ttttgtgtcc cttctcccta tagtgagtcg tattaattat
      541 cccttgacaa gtagagaaaa ctatgagtca aaaacatgac cacacaggtc tgtagtaagt
      601 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      661 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      721 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      781 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      841 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      901 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      961 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
     1021 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
     1081 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
     1141 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
     1201 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
     1261 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
     1321 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
     1381 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
     1441 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
     1501 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1561 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1621 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1681 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctatcaata
     1741 tcattaaaaa gatcgacgta atctatcaga ggctgactgt ctcccgcttc gatacgcctc
     1801 atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc aatgcttagc
     1861 ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg gtcccggtag
     1921 atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc acactcagcg
     1981 atgtccttaa ggtgttccct catttcctca taggtagcgt tgggtctgtc tgcgtgtatt
     2041 gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct atccttggtc
     2101 cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag acggaagtag
     2161 tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact actggatagc
     2221 tgaatcatat gtctgcatgc cctgagaggg ttacacagtg ggttacagta agtaactctg
     2281 caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt taagaaaggt
     2341 c
//
LOCUS       DAJTRW010000233         2292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRW010000233 DAJTRW010000000
VERSION     DAJTRW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..884
                     /gene="proB"
                     /locus_tag="OO326_004169"
     CDS             <1..884
                     /gene="proB"
                     /locus_tag="OO326_004169"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5968308.1"
                     /translation="LAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNAR
                     DTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYT
                     ADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIA
                     AGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERG
                     SSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAIL
                     GYEYGPVAVHRDDMITR"
     gene            896..2149
                     /gene="proA"
                     /locus_tag="OO326_004170"
     CDS             896..2149
                     /gene="proA"
                     /locus_tag="OO326_004170"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5968309.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          542 a          575 c          668 g          507 t
ORIGIN      
        1 tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt
       61 atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct
      121 tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca
      181 atgagaacga tgctgtcgct acggcagaga ttaaggtcgg cgataacgat aacctttctg
      241 cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt
      301 tgtataccgc tgatccgcgc agaaatccgc aggcagaact gattaaagat gtttacggca
      361 ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca
      421 tgagtactaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg
      481 ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc
      541 tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc
      601 cggcgggtga aatcacggta gatgaagggg caactgccgc cattctggaa cgcggcagct
      661 ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc
      721 gcatttgcaa cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac aacagcgatg
      781 cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg ggatatgaat
      841 acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct ggccgatgct
      901 ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg
      961 cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat
     1021 cctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta gcgaagcgat
     1081 gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg atgtacgtca
     1141 ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac tggacagcgg
     1201 cctgcgtctt gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg
     1261 cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct
     1321 gcgtggtggt aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga ttcaggacgc
     1381 cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc
     1441 gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgtggtgg
     1501 cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca caggtggtat
     1561 aggcgtatgc catatttacg ttgatgaaag tgcagagatc gctgaagcat taaaagtgat
     1621 cgtcaacgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa
     1681 taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt
     1741 gacattacac gcagatgcag ctgcgctgac acagttgcag gcaggccctg cgaaggtggt
     1801 ggcggttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat
     1861 cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac aacactccga
     1921 tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc
     1981 cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg gtctgggtgc
     2041 ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact
     2101 gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg
     2161 tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg ttagttttaa
     2221 ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt
     2281 ggtagagcag cg
//
LOCUS       DAJTRW010000234         2250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRW010000234 DAJTRW010000000
VERSION     DAJTRW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO326_004171"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO326_004171"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5968310.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAJTRW010000235         2240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRW010000235 DAJTRW010000000
VERSION     DAJTRW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO326_004172"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO326_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5968311.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..>2240
                     /locus_tag="OO326_004173"
     CDS             2176..>2240
                     /locus_tag="OO326_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="cytoplasmic mutarotase that catalyzes the
                     conversion between beta-pyran and beta-furan forms of
                     D-ribose; RbsD is required for efficient ribose
                     utilization in E. coli; rbsD-mutant E. coli strains are
                     unable to use ribose as a sole carbon source; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5968312.1"
                     /translation="MKKGTVLNSDISSVISRLGHT"
BASE COUNT          465 a          528 c          600 g          647 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga
//
LOCUS       DAJTRW010000236         2209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRW010000236 DAJTRW010000000
VERSION     DAJTRW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..203
                     /gene="relB"
                     /locus_tag="OO326_004174"
     CDS             <1..203
                     /gene="relB"
                     /locus_tag="OO326_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5968313.1"
                     /translation="ARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAE
                     LVEIVKERLRNPKPVRVTLDEL"
     gene            203..490
                     /gene="relE"
                     /locus_tag="OO326_004175"
     CDS             203..490
                     /gene="relE"
                     /locus_tag="OO326_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5968314.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            562..717
                     /gene="hokD"
                     /locus_tag="OO326_004176"
     CDS             562..717
                     /gene="hokD"
                     /locus_tag="OO326_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5968315.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            935..1186
                     /locus_tag="OO326_004177"
     CDS             935..1186
                     /locus_tag="OO326_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968316.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1253..1531
                     /locus_tag="OO326_004178"
                     /pseudo
     CDS             1253..1531
                     /locus_tag="OO326_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1533..>2209
                     /locus_tag="OO326_004179"
     CDS             1533..>2209
                     /locus_tag="OO326_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5968317.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          558 a          534 c          588 g          529 t
ORIGIN      
        1 aagcgcgttc ttacgccgcg cttgaaaaaa tgggtgtaac tccttctgaa gcgcttcgtc
       61 tcatgctcga gtatatcgct gacaatgaac gcttgccgtt caaacagaca ctcctgagtg
      121 atgaagatgc tgaacttgtg gagatagtga aagaacggct tcgtaatcct aagccagtac
      181 gtgtgacgct ggatgaactc tgatggcgta ttttctggat tttgacgagc gggcactaaa
      241 ggaatggcga aagctgggct cgacggtacg tgaacagttg aaaaagaagc tggttgaagt
      301 acttgagtca ccccggattg aagcaaacaa gctccgtggt atgcctgatt gttacaagat
      361 taagctccgg tcttcaggct atcgccttgt ataccaggtt atagacgaga aagttgtcgt
      421 tttcgtgatt tctgttggga aaagagaacg ctcggaagta tatagcgagg cggtcaaacg
      481 cattctctga accaaagcat gacatctctg tttcgcaccg aaggtgacac ttctgctttg
      541 cgttgacagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc cctgatcgtc
      601 atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg cgaggtacga
      661 atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga ggagtaagag
      721 acccggcgag tgagaaatcc ctcgccacct ctgatgtggc aggcatcctc aacgcacccg
      781 cacttaaccc gcttcggcgg ttttttgttt ttattttcaa cgcatttgaa gttctggatg
      841 gtgccggaat agaatcaaaa atacttaagt agcgcgcagg gataaaaggg atggaccccg
      901 aacaggggag tgctacttat ctggaaggat tctgatgatg aacatcgaag aactgcgtaa
      961 aattttttgt gaagatggcc tctatgctgt gtgcgttgaa aatggaaata ttgttagtca
     1021 ttaccgcatt gtgtgtttgc aaaaaaatgg ggctgcgtta attaattttg tggatgcccg
     1081 agtgacggac ggatttatct tgcgcgacgg tgagtttgtc acttcattac aggcattgaa
     1141 agagatcgga ataaaagctg gcttttctgc tttttcagaa gaataaactc atctacaatc
     1201 ttgcgcgggg ctgaactccc gctgagtaac accgtgccac cggagaaaac cgatggcacg
     1261 caacgcaaaa tattacaatc atgataattc gaccgttctt gcccacacgc acgagcggta
     1321 ttctcacgca tttaagtcag actggtacca gcatccctca tgcactgaag aacaggccga
     1381 atggcttatt cagtgttacc gcaggcgcgg atgcgaggtt aaaaaagccc ttagcctcga
     1441 ctaccgtcac tagataatct ccgtcaggct cccttactcc gaacagccac cgcgtccgtc
     1501 ccgcacattc cagcaacgga tctggaggta acgtgcgggt attacttcga cctgttctgg
     1561 ttccggaact cgggctggtg gtccttaagc ccggtcgtga atccatgccg gtattccaca
     1621 atacccgggt actggtggag ccggaaccga aaagcatgcg taatctgccg tccggggtcg
     1681 ttcctgccgt tcgccagccg ctagtggaag acaaaacatt gctgccgttt ttcagtaacg
     1741 cacgggtgat tcgtgctgct ggtggtgctg gcgcattgtc tgactggctg ttgcgccata
     1801 ttaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtc attcaccgtt
     1861 atggtaccgg cgcaatggta ttgtgctggc actgcgacaa ccagctgcgt gaccagacat
     1921 ccgaatcact cgagcaactt gctcatcaaa acctgtcagc atggatgatt gacgtcatcg
     1981 gtcacgcaat aagcggtacg caggagcgtg aattatctct ggctgaatta tcctggtggg
     2041 cggtccgcaa tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt
     2101 tgcgtgcgga aaaaatccgc tcaatgtacc gtgaaagcga catcgtgccg ggagagcaga
     2161 ccgccaccag catactgaag cagcgcacaa aaaatcttgc gccgctgcc
//
LOCUS       DAJTRW010000237         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRW010000237 DAJTRW010000000
VERSION     DAJTRW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            80..259
                     /locus_tag="OO326_004180"
     CDS             80..259
                     /locus_tag="OO326_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968318.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO326_004181"
     CDS             311..505
                     /locus_tag="OO326_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968319.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO326_004182"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO326_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTRW010000238         2151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRW010000238 DAJTRW010000000
VERSION     DAJTRW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO326_004183"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO326_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5968320.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO326_004184"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO326_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO326_004185"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO326_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJTRW010000239         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRW010000239 DAJTRW010000000
VERSION     DAJTRW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO326_004186"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO326_004186"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5968321.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2095
                     /locus_tag="OO326_004187"
     CDS             1647..>2095
                     /locus_tag="OO326_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5968322.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAG"
BASE COUNT          568 a          438 c          542 g          547 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaa
//
LOCUS       DAJTRW010000240         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRW010000240 DAJTRW010000000
VERSION     DAJTRW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..776
                     /locus_tag="OO326_004188"
     CDS             join(<1..274,274..776)
                     /locus_tag="OO326_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5968323.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSSAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIRALKCAAGGSKKHPDIDGD
                     NGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKR
                     RGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVER
                     CFGRLKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
     gene            complement(946..1695)
                     /locus_tag="OO326_004189"
     CDS             complement(946..1695)
                     /locus_tag="OO326_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5968324.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          618 a          450 c          480 g          546 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aatgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgcaca gcttgatcgt gatgcgtact gcaatcgtaa tgtcgttgaa cggtgctttg
      661 ggcggctgaa agaatatcgc cgcatcgcca cgcgttacga caaaacggcg agaaattacc
      721 tggcgatagt gaaactgggc tgcatccgac tcttttatca acgcttacgt aattaaggga
      781 cacagcctag tgaaattacc gacaagaagg gaaagggcct ttccgagagt ggtaaaggag
      841 aggcactgga aataccccac agccctgaaa aagagccagt cagttgctta actgactggc
      901 attagccctg aaggtcgggg ttataaggcg tactggctaa aagcattatt ttttctgatt
      961 gttagcgaat tcatctttcg ctttttgtaa ttgtgcctgg aacacaggat ttgagtgcag
     1021 tgttgctaca atagcagagc caacaatacg tgctgcgtca acatcacttt gccagtgata
     1081 accgcaaata accctgctgt cccccagttc ataaccccgt ttcagaatgg tatcctggcg
     1141 tgccggattt atttcggaca atacaagtgc tgttgcccaa ccaatcgatg tatgacctga
     1201 tggataagag ccatttctgg ataatgtatc ctgttctttt gtattacacg tagaaacacc
     1261 ataaaaagca aaaggtcgaa tacgcatata atattctttt gctgaacgcg ttgccaggtc
     1321 tccggcatcc tcaatcatat ttgtcagtaa tttatgcaat tctggtgaat ctttagccgt
     1381 tatcggagaa ccaaaagcag cagaaaatac atttgccacg ccaccactac tgagattggc
     1441 atcctcagct gccagcttcc ctctttcagt atttcgcaat aaacgcccct tttcatacat
     1501 ggcctggtca ttcaggaagg caatacttcc gatttgtggt ggaggcggta ataatgccag
     1561 actgtcaata gcattatcgt ttgtcaggta gtaaaggtca ggttttgttg tcacatcatt
     1621 tcccggaggg aatgatgcaa aggtatttaa agaaaatacc ccgacaatac taagagtaaa
     1681 aagctgtctt ttcatataac atctccttat agaggataca tatttataat tattttatta
     1741 agagcataaa gtactggata ataaaattgt gtgtactatc acataattta aataaatcag
     1801 aagatgctaa aacaaaggtt acctattaaa tatccgttat gaacaagcat aaaaaggagg
     1861 acgtttaaaa cacccagaac aaacagttag aattaagttc ttaccaacaa ggggaataag
     1921 caacttgtat ttttgagttg cttttacaga caatgactcc aattagtaga acatatgttt
     1981 ttcgataaac gcaccgataa ctttttgagg tagcctgagt ttaacggaca ctccttcctg
     2041 aaatagaatg gcatcagaag gagccaatat ttacgggtaa agccctgttt atgg
//
LOCUS       DAJTRW010000241         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRW010000241 DAJTRW010000000
VERSION     DAJTRW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..799
                     /locus_tag="OO326_004190"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO326_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO326_004191"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO326_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO326_004192"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO326_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          438 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DAJTRW010000242         2057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRW010000242 DAJTRW010000000
VERSION     DAJTRW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(53..322)
                     /locus_tag="OO326_004193"
     CDS             complement(53..322)
                     /locus_tag="OO326_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5968325.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            379..1047
                     /locus_tag="OO326_004194"
     CDS             379..1047
                     /locus_tag="OO326_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5968326.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1363..>2057
                     /locus_tag="OO326_004195"
     CDS             1363..>2057
                     /locus_tag="OO326_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968327.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          620 a          453 c          382 g          602 t
ORIGIN      
        1 gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt tttctttttc gcttacattg
       61 tgtctggttc ttccggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca
      121 acacccgata cttcttccag gcttccagca acgagttttc ttcctccgtt gcgatctcca
      181 gatctacagc atcctgaagt ggcgcaatat gctcactgaa tccctggatg tagaactgtg
      241 tggtgacggt cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa
      301 tatcgctatg ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt
      361 cacaaataaa actataatat ggtgaaatta atgaaaaaaa acacagatga tggggctaaa
      421 atttacacac cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg
      481 cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga acatttaggt
      541 aacaaccatc tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt
      601 agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct
      661 ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgaacctta tcctgcggcg
      721 ttacatggtc aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat
      781 atatctacaa aaagctgtgt aatacgcaat gcggcgcagg ccttaactga tgatggaact
      841 ctatacggag ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg
      901 atgcgcattt acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta
      961 acacatatac tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta
     1021 atgttttccg cttcagggaa aaaatagcat ccaaccgcag cacgttcttg cttaagacgt
     1081 gctacggttt cctttacgat ttggggttgc gactttacct ctattgataa tgcattccgg
     1141 cagaacgttc aaatatgaac gtacgatatt taactaaccg aaaaacaaaa tatagcacag
     1201 gcgagacatt taccatctaa gaaaatagta tcgttttttt acagttaaat caacatcact
     1261 tccttaaaat gaaaacaata aatttcaatc ctgaaacatt ttatttcagc ttattattcc
     1321 ccagtatcac attttatcag gatatccttc tgcgaggtta ttatgcttcc tgtaaataat
     1381 ccccccctat ccactggaaa cgtctctttt tacagaacta catcaatcga caatgttcac
     1441 aataattatc tctccgaatg ggttgaatgg actaaaaaca gcatttccgg agaaaacagg
     1501 gaaactgctt ttacccggct ccaattatgt ctggagaaca gtgaaacatc gttggactta
     1561 tcttgtttag gtctcagatc tctaccacga ttgcctgaca atcttgatga aattaatgta
     1621 agcaataacc aactatcaat gctccccgag ctaccaaggg cattgaaaga gctgaatgca
     1681 agcagtaatc aattatctgc acttcctgaa ttaccagtgt cgctggaata tataaatgtg
     1741 agtgataacc atttgttcgc acttcctgaa ttacctgcgt cactagaata tattaatgta
     1801 agtgacaatc acctgtctgt acttccgagg ttaccaatgt cattggaatt acttgatgca
     1861 gccagaaatg ctttggaagt aataccagat tttccagaaa gagatgatca tattataaga
     1921 atattctggc ttaatcagaa ccggatcacg gcaattccgg aaagcatact tggcctcagt
     1981 tctgatagcg ttgtcaatct tagagaaaat caactatctc ccagaataat gcaaactttg
     2041 ttacaacaaa ccgccca
//
LOCUS       DAJTRW010000243         2052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRW010000243 DAJTRW010000000
VERSION     DAJTRW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..665)
                     /locus_tag="OO326_004196"
     CDS             complement(<1..665)
                     /locus_tag="OO326_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5968328.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
     gene            complement(616..1380)
                     /locus_tag="OO326_004197"
     CDS             complement(616..1380)
                     /locus_tag="OO326_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968329.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1575..>2052
                     /locus_tag="OO326_004198"
     CDS             1575..>2052
                     /locus_tag="OO326_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5968330.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYV"
BASE COUNT          499 a          517 c          453 g          583 t
ORIGIN      
        1 gccgcatcat catcagactc cggaacgccc tcatacacat gtttaaaacc atccggatca
       61 cggcgtcgcg ccgcgtcgat aaccttaagc atggtgtcag acagaaacgg attttcgtca
      121 tagttaattt tgcggatgag agtgccttcg ggcggatcaa caacgaagtt gcgccagacg
      181 aaatcagtaa caagtccggg gttgaatatg aaccagcatt ccgaaccctc tttacggatc
      241 gttggctcca gaattttcca ctggtattcc gtcagtgcgt gggcttcttc caaccacaac
      301 atatcgatcc cctccagtga cttaatttct tcgatgttgc gccataatcc ataaaaaaca
      361 aattccgagc cagtaacccg gttaatgatt ttgttgttca gaatacggaa acggtgccgc
      421 agaccaaacc tgtctatctg aattttgagc agggtataca ccgactcttc gattttattc
      481 tggatctgac gggcacaaca aaaacgcagg gtgtatttat tcgacagaaa tatggcaatg
      541 ccagcggcat cccatgattt tgacgatgac cgaccaccat aaagcacttt gttacgtgcc
      601 cgcatagtcc agaaactacg taaagccgga ttcagcgtcg gtttggatgt cagagtagaa
      661 gtcattgagg tcacgctctc cattgccatc atcaatacct gcatcacggc gaagacgatc
      721 agcctccaga gacaccttat cagtggcggc cttgcggtag gctgtatcag caaatatttt
      781 tcctaccgtc gcaagcgtgc caacgatgga ctcaatacga actgtattgc gcatcattgc
      841 tttctcggcg gcgctgatat tttcaatcag catcttcctt tcctggtccc cattagcatc
      901 ttccagcgac accaaccacc gaccaatatt ctctgcagcg acaaggttgt tagcacgaag
      961 gcgaaataat tcgtcctcga gcgtcaacgc tttagcgtcc tctatcacct catctttgag
     1021 cagaaggcga cgggcataac caccgtgttt taatgcctgc tggttaccgg gttggaatgg
     1081 gttagtcggg ggatcggtac gcattccgcg tatcggtttc gtatccggtg tagattctgt
     1141 ttttagttgc gtactttttt gtgtaaggcc agtaatggca ggctttctgc tggtacgcac
     1201 tttccttttt tgcgtaccat ttttgcaaac ctgcgtaccg ccactgcgtg cccaatcaag
     1261 ctttttggcc ctcttcctga tagccccttc tgtaacgccg tatttctcgc ctatatcacg
     1321 gaggctaagg actccggccc ggtatgccga ttcgatggcc tcccagtccg gttttgccat
     1381 gattatgctt cctgtgattt actgttatcg ctgcctgtca caataggttg ctttaattct
     1441 tcctcatcac tcactcctgc gacgctccgt ggcagggccc cccacaggag taccttgtaa
     1501 atgtaaggtg tttaagccta aattaagtcg tatcacggtt aaatagcaaa gaaaaattca
     1561 cagaaggaaa gacattgatg gagtggataa atgctttgat gaactatttt gtacttcatc
     1621 catatcgcct atgggggctt ctttttataa tagcgttcac aaaatccact ttactcattt
     1681 cggcgatatt gccaccagca tcaattatga tggccactgt aattacagtc agcaagacga
     1741 ctctttcacc atgggaagct ggaatcacgg tcatgagtgg cgcatggtgc ggtactatcg
     1801 ttaattacca tcttggtata ataattgggc atatccctca acttgcatgt ataatatcga
     1861 gccgttccaa tacaatagaa cgggttcgcc ttcagttgca aaataactct gtgtcaatac
     1921 ttttcacttc gcgctttatc gccgtgctgc gttacattgc tcctttggtg gcaggaatgt
     1981 tgcaacttca tccagtgaaa gtgtatacag tgagtctgat ttcagctgct agctggtcag
     2041 cactttatgt tg
//
LOCUS       DAJTRW010000244         2033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRW010000244 DAJTRW010000000
VERSION     DAJTRW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..363)
                     /locus_tag="OO326_004199"
     CDS             complement(<1..363)
                     /locus_tag="OO326_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5968331.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OO326_004200"
     CDS             complement(430..1644)
                     /locus_tag="OO326_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5968332.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          493 c          413 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcta ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttgaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggtcacca gaa
//
LOCUS       DAJTRW010000245         2011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRW010000245 DAJTRW010000000
VERSION     DAJTRW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..2011
                     /gene="sat"
                     /locus_tag="OO326_004201"
                     /pseudo
     CDS             1..2011
                     /gene="sat"
                     /locus_tag="OO326_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          740 a          350 c          393 g          528 t
ORIGIN      
        1 tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac agcggctctg
       61 gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca acccatggta
      121 ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat aaactggtta
      181 gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat gtaaccatta
      241 aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact caagaaaaaa
      301 taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt aaggataatt
      361 tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac ataaacggtc
      421 agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt gtaaactgga
      481 atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact ctgaatgttc
      541 aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt aatgaagaag
      601 gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta aataaagata
      661 attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt ggcacgctag
      721 atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat ggaacaacaa
      781 taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa gactcctaca
      841 tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat tctcaggata
      901 agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat gatgttgaag
      961 tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata ttcagaagca
     1021 cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc gttttgaaag
     1081 aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat aatcagcaga
     1141 cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca ttacacctga
     1201 acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata tcagcaaata
     1261 aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca gggaaaaata
     1321 ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc ataggagaaa
     1381 ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt gatcaagctg
     1441 tagttacact taatggtgca acctttctga ataatactcc tataagtata gataaaggag
     1501 caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc tccggtgaac
     1561 tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt ctccactacg
     1621 ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga aatatggcat
     1681 ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga cagcctgaaa
     1741 ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt ttgtatggct
     1801 ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc atgaataatg
     1861 cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa gacagtatgg
     1921 tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac cttactatag
     1981 atgacagtgc atttgtgctg cgtgcaaatc t
//
LOCUS       DAJTRW010000246         1986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRW010000246 DAJTRW010000000
VERSION     DAJTRW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..684
                     /locus_tag="OO326_004202"
                     /pseudo
     CDS             1..684
                     /locus_tag="OO326_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            686..1291
                     /locus_tag="OO326_004203"
     CDS             686..1291
                     /locus_tag="OO326_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5968333.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1278..1826
                     /locus_tag="OO326_004204"
                     /pseudo
     CDS             1278..1826
                     /locus_tag="OO326_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          488 a          472 c          545 g          481 t
ORIGIN      
        1 tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac gtaattcagt
       61 tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct ccacggtaga
      121 aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa aagccggagt
      181 cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag cccggcagat
      241 atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg caacacaacg
      301 ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat cagctggcag
      361 acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga ctacctgcct
      421 tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg tacagatggt
      481 ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc cggcatttac
      541 aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga tggttcgtca
      601 tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt gtcggatatc
      661 agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc ttgcgcagta
      721 tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc tttcccccga
      781 cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag aaaagtatgg
      841 tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa aggacgattt
      901 taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg atccctgtcc
      961 gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg acctgtctga
     1021 tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg actgttccgt
     1081 tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg cttatgttct
     1141 gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt tggcagaact
     1201 ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg tttcagtagt
     1261 tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg gtcccctttg
     1321 cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc agagagagcg
     1381 gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc tcagcgggag
     1441 gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc gcagcaatac
     1501 ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg gccattggtg
     1561 gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg ctgaatacca
     1621 cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta aatcttcttg
     1681 aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt cagaataaag
     1741 ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa cgctatgttc
     1801 atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat gcgctgagga
     1861 ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg caccttattc
     1921 ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg ttcaccatta
     1981 atttgc
//
LOCUS       DAJTRW010000247         1964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRW010000247 DAJTRW010000000
VERSION     DAJTRW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO326_004205"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO326_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5968334.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          332 c          311 g          699 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAJTRW010000248         1930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRW010000248 DAJTRW010000000
VERSION     DAJTRW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..862)
                     /locus_tag="OO326_004206"
                     /pseudo
     CDS             complement(1..862)
                     /locus_tag="OO326_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          515 a          454 c          452 g          509 t
ORIGIN      
        1 tggagattca gggggccagt ctagcagctg ctctacggtc atatttttaa ttgcgctccg
       61 gtttacaaga gcccacccct gtttttccag ataaaactgg aaagtatcca gggtacagac
      121 cagtgcaccg tcaggaacag tttcggtgaa tctgatattg ccgtgttcgt cgaagtgcac
      181 aaccagagtg cgaccgttac ccggaatcat cttgtcagcg gacggggtat tattctggtg
      241 cagttctgcc tccatgcggt cgaacatcag cgatgtaggc ctctttgaat gcagcggctt
      301 ttttcccagt gaagcccatc accaggaaaa cgaagccgtt tttggtgatt tggtacattg
      361 ggcgtttttc gcctttggtg tcggtgtagg tgacgtcctc aaaattgagg gcgttaaatt
      421 caggtgagca ttcagtattt gcgatggcac gtaacacatt gtcgtgtcgt ttgtggaaga
      481 actctgcaac cgcaacagac gtagtgacag cgcgaccgtt ttcgatggtt acgtcagggt
      541 gagaaagggc agggatagta gccatgatgg catcctcgag tgataagtta attaactcac
      601 caccgaggtt ttccacgacc ataagggtgg tgagacgtac aggggtggaa ataccggtca
      661 ctcgagaacc cggccagtct tgcgactgcc ctgcacatcc caccataatt tgaatggagc
      721 tgtgcattac gcataaaaaa accgcttcag cgcggctatg cgctcgagta acgttcgggt
      781 ttccacgccc ggcccccgtt ttatgaggtg cagatgcact ataattccac ccgttctggt
      841 tttcaatagc tacattcaac attttctctt acctttcatc accgaagtga actttgttga
      901 tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc ggatacaggg
      961 aaacccataa ccacccgata cgtgggaact aatcgctttt taactgttcc gcgtgcgctc
     1021 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggacatcag ccagcaatga
     1081 aactgatgcc gccaaaggta ccaatcaaca tggagtgttg tggcgtggtt gtcacttaag
     1141 cgtatggtca acctgacaac ccggtgtcct caacggggaa ggaataactc cgccatactt
     1201 accgccgcgt catttcgcgt tgtgtgcctg cttttaacta cgtcaggcga ggtggttcct
     1261 gttattcccc aacaacaaga aatttgtata atctgaatac cccaacaata agaagagtga
     1321 gcaagtgatg tattagcgat gtcagctctc aaagagactg catcaatgtt aaaattattc
     1381 aatgaagcaa aaactcaggc tgaggttgat cgcgctgtgt ctgaaataaa ctctaagttg
     1441 tcaagcattc agcttgaata tttgtcatct cattgatgag ctgacaatct ctaaaagcaa
     1501 tgaggctgcg ctcaaagcaa aactcacgga gatagaaaag tttaagttcc aaagtgaaga
     1561 ctgcgaacct cgctaattga ttactggaac gattgtgtat gccagacagt gcaatactag
     1621 ctctggcaag attaccatgt atttttgtcc agaatgtttt gcgaagagag caatatctat
     1681 attgcaacct ttccctatcg aggaaggcga taaatatacg aaaactcaat gcccctcctg
     1741 taaaaatgag tatctcatgg actacaattt caataatgat ttttgagtca cggtattcag
     1801 atttttaaag aacatgccgg atactcaccc gtgtccggcg cacgcactcc acctcacccg
     1861 tggagaactc cttaattacc aaccttggct tcgttggtta gccattaacg ctggtatgta
     1921 atcattctgg
//
LOCUS       DAJTRW010000249         1916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRW010000249 DAJTRW010000000
VERSION     DAJTRW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(56..1705)
                     /locus_tag="OO326_004207"
     CDS             complement(56..1705)
                     /locus_tag="OO326_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5968335.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          508 c          491 g          465 t
ORIGIN      
        1 cctgaagagg ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg
       61 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
      121 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      181 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      241 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      301 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccaaaa
      361 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      421 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      481 cgcgtcgcca gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta
      541 ccgcatcgcg acgggcactg gccttctggc ggaacagcgc ttctgtctct ttctcgcctt
      601 tcagcgccga gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga
      661 tggggatgaa ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg
      721 aagagtaatc ccaaccggag gccgctcccg cgcgtaaatc gcggtacacc tcgttgggcg
      781 ggcgaccaga atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt
      841 cgcgatcgtc ccagtaacgg ttgagcagcg atccgtccgg catccgcaca acatggcgat
      901 aggcctgatt agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttaa
      961 ggtggtcgag atagcggcgc gcaccgcgta caccatcttc ttcaaacaac tccaccatca
     1021 gcgcaaaaac cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt
     1081 gaccgtagtt ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc
     1141 gaccactttc cgccagtccc agcatggtga aataggaatc ccaatagtag gtttcgctaa
     1201 aacggccgcc cgggacaata tatgactgcg gtagcgccag cagagaagac cacggaatgt
     1261 gatcctgtgg ttcgcgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat
     1321 tttgcgggtc cgatacatac tcgctggagt agacctccgg cagccagaag tgattttcaa
     1381 caaacttgcg caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat
     1441 ccagcgggtc cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg
     1501 ccgactgaac atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg
     1561 cgggcagccc ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta
     1621 attcatacgg gtccagttca tagcagagat cgacctcgat catcagttcg tctggattag
     1681 ggttttgaat tttctgattg agcataagga tccaacctcc gaaattcgtc gctaattatc
     1741 gggcttttct ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga
     1801 tcacgtttat taacttgtca aaaaatataa cgcactaata tcaatggaat ttataaaaat
     1861 acggatttgg tgttgttttt cattttcaga acattcaatc ctgctctcgc cgcaat
//
LOCUS       DAJTRW010000250         1839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRW010000250 DAJTRW010000000
VERSION     DAJTRW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..302
                     /locus_tag="OO326_004208"
                     /pseudo
     CDS             1..302
                     /locus_tag="OO326_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            471..1835
                     /locus_tag="OO326_004209"
     CDS             471..1835
                     /locus_tag="OO326_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5968336.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          479 a          351 c          414 g          595 t
ORIGIN      
        1 agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga aataaaacca
       61 gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact acaagatttt
      121 atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt gatacttaat
      181 gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat cttaaacatg
      241 caagtaattt tggtattctc gatacaagaa taaatagtag taactactat agctgcaaat
      301 aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa ttgtttttgg
      361 ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta tataatgata
      421 agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat atgtcattat
      481 tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga ttatatcccg
      541 tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat tattttgtcc
      601 gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag atcggcatta
      661 tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga cttatttctg
      721 acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga ctggtaaatt
      781 ttctgatagg atatattccc gggatcatca catccgttac gttattttca acaatgtatt
      841 tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca ctggtgttct
      901 ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg cacaatgtcg
      961 ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc gacagtgctc
     1021 tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca atgagtgtgc
     1081 tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc attgaagaat
     1141 ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg agactctcca
     1201 ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac tgctgtatct
     1261 atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct aagtttttaa
     1321 gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg ggggggggtt
     1381 ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg tctgtcagac
     1441 tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc attattttat
     1501 taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac tatacaatcc
     1561 ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt tattcaatgg
     1621 agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca ttttcctata
     1681 tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat ctgggatggg
     1741 gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc acactgatga
     1801 gtcgtgaacg ctcactggag ttcgaaaaag agtaatttt
//
LOCUS       DAJTRW010000251         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRW010000251 DAJTRW010000000
VERSION     DAJTRW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(208..609)
                     /locus_tag="OO326_004210"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO326_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO326_004211"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO326_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5968337.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTRW010000252         1804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRW010000252 DAJTRW010000000
VERSION     DAJTRW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            178..1203
                     /locus_tag="OO326_004212"
     CDS             178..1203
                     /locus_tag="OO326_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5968338.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1271..1804
                     /locus_tag="OO326_004213"
                     /pseudo
     CDS             1271..1804
                     /locus_tag="OO326_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          420 a          421 c          461 g          502 t
ORIGIN      
        1 tttcgcacca ttgcggggcg ctgtttttat tatttcctgt ctacactcag cttattgcga
       61 cgcgaaatcg tgcgcaaaag ctggcactac ttttgcttat aagaggatgg ccacagacag
      121 gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg aagcattatg
      181 aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag
      241 gcgcatgctg gcgcgacgct tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc
      301 agtgatggat tacctaggtt ctcttatgcc gatgcagacg gtaagttttc aggtattgat
      361 gttgatgttt gtcgtggtgt tgccgctgct gtatttggtg acgacacgaa agtgaaatat
      421 accccactca cagcaaaaga acgcttcacc gctttacagt caggggaagt ggatttgctc
      481 tcccgtaata cgacctggac ttcatctcgc gatgccggga tgggaatggc atttaccggc
      541 gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct aaaaagcgcg
      601 aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga actcaacgtc
      661 gccgactatt tcaaggcaaa caatatgaag tacacaccag tgactttcga tcgctctgac
      721 gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcttcgga tcagtcgcaa
      781 ctgtatgcct tgcgcatcaa attaagcaac ccggctgaat ggattgtctt accggaggtt
      841 atctctaaag aaccgcttgg gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt
      901 gtacgctgga cgcttttcgc catgctaaat gctgaagaga tgggcatcaa ttcccagaac
      961 gtcgatgaaa aagcggctaa tccagcaacg cctgatatgg cacatctgtt gggtaaagaa
     1021 ggcgattacg gcaaggatct gaagttggat aataaatggg cctataacat catcaaacag
     1081 gtgagtaact actcggaaat ttttgagcgt aacgtaggtt cagaaagccc gctgaaaatt
     1141 aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc gcccgtgcgt
     1201 taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg ttatgctttc
     1261 cgaggtttct atgcctcatc gccgctcaac cgttaaaggc tcactctcct ttgccaaccc
     1321 tacggttcgt gcctggttat tccagatcct tgccgttgtt gctgttgtcg gcattgttgg
     1381 ttggttattt caaaacactg taacgaatct caataatcgt ggcattactt caggttttgc
     1441 ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt accagcaggg
     1501 cgacacctac ggacgcgttt ttattgtcgg cttactcaat acgctactgg tttctgcatt
     1561 gtgtattgtg ttcgcttctg ttttgggctt ctttatcggt ctggcgagac tttcggataa
     1621 ctggctgcta cgaaagcttt ccacaattta tattgagatc ttccgtaata ttgcaaatct
     1681 tcttctggta ctttgccgtg ttgcgcaatt tgcccggacc acgccaagct gtgagcgcgt
     1741 ttgatctggc ctttttgagc aatcgtgggc tttatattcc gtcaccgcag ctgggagacg
     1801 gatt
//
LOCUS       DAJTRW010000253         1773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRW010000253 DAJTRW010000000
VERSION     DAJTRW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..165
                     /locus_tag="OO326_004214"
     CDS             <1..165
                     /locus_tag="OO326_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5968339.1"
                     /translation="GSAGYWFLQWLDQVRPSQWAAIGVLGSLLLGLLTYLTNLYFKIR
                     EDKRKAARGE"
     gene            169..798
                     /locus_tag="OO326_004215"
     CDS             169..798
                     /locus_tag="OO326_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968340.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            864..1397
                     /locus_tag="OO326_004216"
     CDS             864..1397
                     /locus_tag="OO326_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5968341.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1614..>1773
                     /locus_tag="OO326_004217"
     CDS             1614..>1773
                     /locus_tag="OO326_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968342.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDE"
BASE COUNT          471 a          391 c          487 g          424 t
ORIGIN      
        1 ggcagtgccg gctactggtt tttgcagtgg ttggatcagg tcaggccatc acagtgggct
       61 gcgattggtg tgctgggaag tctgcttctg gggcttctga cttatctgac gaatctgtat
      121 ttcaaaataa gagaagacaa gcgtaaggct gcacggggag agtaaataat gaagcatgaa
      181 gaaatgaatc agcgattcaa tcacctggaa aatgaaatca ctgagcttaa taaaaaattg
      241 tcggcgttgg tgagttctga agatgaaaac aaacgccgcg atgagcatta tgcggcgatt
      301 tacgattact gccacaaagt cgctcacgag acttttatga agtttttgca ggaaaagttt
      361 ttaccggccg cattgtcaga aaaagaggcg gcttacctgc ggcctgagta cgtcattacg
      421 gttaacagtg ccggagagga agagcataaa agtgatttta ttgcgtctgc accggataaa
      481 gaccaggaac cccatcggcc tttcagggtt tcctgtgaag agggtgagtt cgtcgtttat
      541 gagaacggaa aacctgtccg ggcatcgcat catcactgcc tgaaaattat taatctcgct
      601 atcagatgcc tgaaagacga aaacaccaga gttatgaaac gcattggccg ttgcatgggg
      661 tatttgcagg tggcggcaga gattgaagca cttgccagtg gtgcagacat ggatgctgcg
      721 gtgcgggagg ctcttcttcg tgatttcaat actccccctt taagaaagag cctgatgact
      781 ggatccagcc ggggctgact tatcttaaaa ggcgtatata agttggctcg ttatttgttg
      841 ccgataaatc ctgataaata tccatgagcg caaaaatcaa atacggcctg tcggctgctg
      901 ttctggcgct gattgccgca ggcgcgtctg ctccccagat acttgaccag tttctgaacg
      961 aaaaagaggg taatcacaca acggcatacc gtgatggttc tggcatatgg accatctgtc
     1021 gtggtgccac aatggtggat ggtaagcctg tcataccggg aatgaagctg tcgaaggaaa
     1081 aatgtgacca ggttaacgct attgaacgtg ataaggcgct ggcatgggtg gagcgcaata
     1141 ttaaagtacc tctgaccgaa ccccagaaag cgggtatagc gtcattttgc ccctataaca
     1201 ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gctgaatgcc ggtgatcgta
     1261 agggcgcatg cgaggcgatt cgctggtgga taaaagatgt tgggcgcgat tgccgcatac
     1321 gttcaaataa ctgctatgga caggttattc gtcgtgacca ggaaagcgca ttagcctgtt
     1381 gggggataga tcaatgagca gagtcaccgc gattatctcc gctctggtta tctgcatcat
     1441 cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagagca
     1501 gcgcgacaaa aacgccggag aactgaagct ggcgaacgca accattactg acatgcagca
     1561 gcgccagcgt gatgctgatg cactcgatgc taaatacacg aaggagttag ctgatgcgaa
     1621 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
     1681 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
     1741 caccgtcgaa ctctctccag ttgctggacg aaa
//
LOCUS       DAJTRW010000254         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRW010000254 DAJTRW010000000
VERSION     DAJTRW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(110..577)
                     /locus_tag="OO326_004218"
     CDS             complement(110..577)
                     /locus_tag="OO326_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5968343.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO326_004219"
     CDS             complement(643..882)
                     /locus_tag="OO326_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5968344.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO326_004220"
     CDS             complement(1017..1622)
                     /locus_tag="OO326_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5968345.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTRW010000255         1708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRW010000255 DAJTRW010000000
VERSION     DAJTRW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..180)
                     /locus_tag="OO326_004221"
     CDS             complement(<1..180)
                     /locus_tag="OO326_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5968346.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="OO326_004222"
     CDS             complement(321..875)
                     /locus_tag="OO326_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5968347.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMCDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="OO326_004223"
     CDS             complement(872..1162)
                     /locus_tag="OO326_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5968348.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..>1708)
                     /locus_tag="OO326_004224"
     CDS             complement(1162..>1708)
                     /locus_tag="OO326_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5968349.1"
                     /translation="TPESCDFLHQIKIGEWIHADFKRVRNYAFHKRFFKLLQLGFDYW
                     TPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDTALLKS
                     FDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVLWNWIL
                     FRKFSSPEEVENVAAQLLEFA"
BASE COUNT          415 a          472 c          385 g          436 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cacatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtc
//
LOCUS       DAJTRW010000256         1665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRW010000256 DAJTRW010000000
VERSION     DAJTRW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..430)
                     /locus_tag="OO326_004225"
     CDS             complement(<1..430)
                     /locus_tag="OO326_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968350.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKE"
     gene            complement(1030..1278)
                     /locus_tag="OO326_004226"
     CDS             complement(1030..1278)
                     /locus_tag="OO326_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5968351.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          420 a          293 c          398 g          554 t
ORIGIN      
        1 gctccttcaa agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca
       61 ataatttaag tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa
      121 gttgtgtaag ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa
      181 ttgatttaat tcccgggggg atttcaggta atgttgtcag gcctaattca gacaagtcca
      241 ggttcgtctc ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt
      301 cttcttgtat gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc
      361 ggctagtgtc gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg
      421 tcgggagcat aaatatcagg gtgtattctt tatgttgaga ggtgttggat tcttttttat
      481 ctgctttgca atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga
      541 tatatagttt gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta
      601 cactatccta aactggatcg gtttaatgta aaaaaacgat actttttggg gggggggcaa
      661 tttttcctta aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg
      721 tatcagtgaa tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt
      781 attcttctgg aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg
      841 ttttgctgag actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat
      901 gattggcggg ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg
      961 gctggctggt gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt
     1021 acgtgctaat tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc
     1081 ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt
     1141 gctggtggct tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa
     1201 ttcttccttt aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat
     1261 gcagatttca aaacgcataa aaatccctct gcactgtgtt tttatacagt attattttta
     1321 actgtatgga taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg
     1381 caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac
     1441 tttgccgatt ttcatatgac atggcaatcc aacgctttga ttattctaaa gaaaacacat
     1501 gctcttgggc gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg
     1561 catactcttc aaggctggtg atccccaacc gcacccattt aggatgtgac cattgcggta
     1621 gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacg
//
LOCUS       DAJTRW010000257         1655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRW010000257 DAJTRW010000000
VERSION     DAJTRW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(156..1391)
                     /locus_tag="OO326_004227"
     CDS             complement(156..1391)
                     /locus_tag="OO326_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5968352.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO326_004228"
     tRNA            complement(1538..1614)
                     /locus_tag="OO326_004228"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          450 c          394 g          391 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DAJTRW010000258         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRW010000258 DAJTRW010000000
VERSION     DAJTRW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            240..509
                     /locus_tag="OO326_004229"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO326_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO326_004230"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO326_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO326_004231"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO326_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5968353.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          477 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaa
//
LOCUS       DAJTRW010000259         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRW010000259 DAJTRW010000000
VERSION     DAJTRW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..360
                     /locus_tag="OO326_004232"
     CDS             <1..360
                     /locus_tag="OO326_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5968354.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO326_004233"
     CDS             361..726
                     /locus_tag="OO326_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5968355.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO326_004234"
     CDS             723..1406
                     /locus_tag="OO326_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5968356.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          324 c          438 g          386 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJTRW010000260         1478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRW010000260 DAJTRW010000000
VERSION     DAJTRW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(20..289)
                     /locus_tag="OO326_004235"
                     /pseudo
     CDS             complement(20..289)
                     /locus_tag="OO326_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(404..1042)
                     /gene="ospI"
                     /locus_tag="OO326_004236"
     CDS             complement(404..1042)
                     /gene="ospI"
                     /locus_tag="OO326_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5968357.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1201..1478)
                     /locus_tag="OO326_004237"
                     /pseudo
     CDS             complement(1201..1478)
                     /locus_tag="OO326_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          418 a          366 c          262 g          432 t
ORIGIN      
        1 tggagattca gggggccagt ctacccagca agcgctgtaa ctcttttatt tgttttatag
       61 cggcagccag ttctgaggca ggaacgacat cctctccggc agcaacagca gtgagacttc
      121 cttccagata ttattttttc cacttaagca acaggctggg ctgaatacca tgcaaacgcg
      181 ccacatggga caccgtcatg ccaggctcca ttgtctgctg aataatggca attttttcct
      241 gaggagtttt acgtttacga agctccagcc ccagcaaagt tccagtcatc tcaaaattag
      301 aactagtgtt agacatatat ccaagcctat ctcttatttt aagatacatc cactgtctgg
      361 cgtttcaagg ggccggctca aatctatgta caggctctca gcatcagcaa agcctcttac
      421 ttttccaact actactgtta gcatagtgat caaattcctg atcagataca tgaattacct
      481 gaaccgaact attttcatct acaaatgcat ctggcgattt ttctacagga tcataaagat
      541 taaatccctt tcctgtttga acatccaaaa acataatttt ttcaccatcg ttaaatccag
      601 ctatccaatg agtggcctgt tcaatagaaa tcattactac acttcctttt gaagtatttt
      661 gtaaaatatt ttctgcaata cgctcacgaa atttactata agtttcatta ggatgtgaca
      721 ctatgccttc actatcaatt tgctctaaat gcctatgaaa aaatccggtc atatactctg
      781 atatttcatt aggtacggga gcctctctta ctccataccc taacatagcc atacaagcat
      841 gaagcgcaca ccctgaacag ttaccatccc caccagacgg atttatcatt ttctgtaaag
      901 tcgctgccaa ttcagaatct ttatccatat caccaagact tttagagttt aaagattcca
      961 gcaatttttt aacatttaca tttccctcct ctgtatgtgc ctcatatcca gcagttccct
     1021 gtaacgacac cccattaatc atattgtatc ctgacagtat atatactcac tatattttct
     1081 tcataacaaa tatccctctg atatgacaac aaaaaacgga gccggattcc tgtttttgtg
     1141 aactgcagct attcgtgtct aaaaagtgat ccacttacca ccaacggcct aatatataca
     1201 gaacggagta aacaggacta acagttcccg acaggtctca tcattccgtg gcccgaaagt
     1261 gtaagccaac acaccgccag ttttagtgtt gtacgaatac cagagccagt gttgttgcag
     1321 ccttgctgcc aacgaagcgc cactgctcat caagttcaca gataagggca acatcagcgt
     1381 gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc gtatgacggt
     1441 attgatgccg attttcagtg tcctggcggt atcacggc
//
LOCUS       DAJTRW010000261         1475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRW010000261 DAJTRW010000000
VERSION     DAJTRW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(5..184)
                     /locus_tag="OO326_004238"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO326_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO326_004239"
     CDS             708..992
                     /locus_tag="OO326_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5968358.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO326_004240"
     CDS             992..1267
                     /locus_tag="OO326_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5968359.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          402 a          369 c          362 g          342 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga cattg
//
LOCUS       DAJTRW010000262         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRW010000262 DAJTRW010000000
VERSION     DAJTRW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            82..1275
                     /locus_tag="OO326_004241"
     CDS             82..1275
                     /locus_tag="OO326_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5968360.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OO326_004242"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OO326_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJTRW010000263         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRW010000263 DAJTRW010000000
VERSION     DAJTRW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..312
                     /locus_tag="OO326_004243"
     CDS             <1..312
                     /locus_tag="OO326_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5968361.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
     gene            315..>1438
                     /gene="yiaN"
                     /locus_tag="OO326_004244"
     CDS             315..>1438
                     /gene="yiaN"
                     /locus_tag="OO326_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5968362.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIG"
BASE COUNT          260 a          377 c          376 g          425 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttga tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc
      481 tcctggcgat tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa
      541 aacgcattgt tgacctgccg atgaaactgg tggggcataa accaggcggg ctgggctacg
      601 tcggcgtgct ggcagctatg attatggcga gcctttctgg ttctgccgtt gcggataccg
      661 ccgccgttgc cgctctactg gtgccgatga tgcgcagcgc caactacccg gtcaaccggg
      721 cggcggggct gattgcctct ggcggcatta tcgcgccaat tattccccct tccattccgt
      781 ttattatctt cggtgtttcc agcggattat ccatcagtaa gctgtttatg gcgggcattg
      841 cccctggcat aatgatgggc gcaacgctga tgcttacctg gtggcggcag gccagtcgcc
      901 ttaatttacc ccgccagcaa aaagcaacga tgcaggaaat ctggcactct tttgtctccg
      961 gtatctgggc gctgtttctt ccagtcatca ttattggcgg gtttcgctcc ggtctgttta
     1021 cccctaccga agccggggcc gttgctgctt tctatgcgct gtttgtcgcc acagttattt
     1081 accgtgaaat gaccttcgcc acactttggc atgtactcat tggcgcggcg aaaaccacct
     1141 cagtggttat gtttctggtg gcctcggcac aagtttccgc ctggctgatt accattgctg
     1201 aactgccgat gatggtttct gatttactgc aaccgttggt cgattcaccg cgtctgctgt
     1261 ttatcgtcat tatggtggct atcctgattg tcggcatggt catggattta acgccgacgg
     1321 tattaattct taccccggta ttaatgcctt tagttaaaga ggcaggaatc gatccgattt
     1381 atttcggtgt catgtttatc attaactgct caatcggctt aattacaccg cctatcgg
//
LOCUS       DAJTRW010000264         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRW010000264 DAJTRW010000000
VERSION     DAJTRW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..169
                     /locus_tag="OO326_004245"
     CDS             <1..169
                     /locus_tag="OO326_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5968363.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO326_004246"
     CDS             154..792
                     /locus_tag="OO326_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5968364.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="OO326_004247"
     CDS             1020..>1406
                     /locus_tag="OO326_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968365.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAJTRW010000265         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRW010000265 DAJTRW010000000
VERSION     DAJTRW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(3..485)
                     /locus_tag="OO326_004248"
     CDS             complement(3..485)
                     /locus_tag="OO326_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5968366.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO326_004249"
     CDS             complement(476..778)
                     /locus_tag="OO326_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5968367.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OO326_004250"
     CDS             1050..1322
                     /locus_tag="OO326_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968368.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAJTRW010000266         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRW010000266 DAJTRW010000000
VERSION     DAJTRW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO326_004251"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO326_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5968369.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          228 c          286 g          405 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 ccccttacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTRW010000267         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRW010000267 DAJTRW010000000
VERSION     DAJTRW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            34..435
                     /locus_tag="OO326_004252"
     CDS             34..435
                     /locus_tag="OO326_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5968370.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO326_004253"
     CDS             447..818
                     /locus_tag="OO326_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5968371.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO326_004254"
     CDS             805..>1275
                     /locus_tag="OO326_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5968372.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTRW010000268         1245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRW010000268 DAJTRW010000000
VERSION     DAJTRW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..135
                     /locus_tag="OO326_004255"
     CDS             <1..135
                     /locus_tag="OO326_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5968373.1"
                     /translation="RHGANKQAISHAFDDVEFQERMAEHIRYMAETIAHHQVDIDLED
                     "
     gene            141..1238
                     /locus_tag="OO326_004256"
     CDS             141..1238
                     /locus_tag="OO326_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5968374.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          392 a          283 c          304 g          266 t
ORIGIN      
        1 cgccacggtg ccaataaaca ggcaatcagc cacgcatttg atgatgttga gtttcaggag
       61 cgcatggcag aacacatccg gtacatggct gaaaccattg cccaccacca ggttgatatt
      121 gatttggagg actaaaatct atggcgcttt ttcaacgagc aataaacaca caggcctatc
      181 taaaagcagg aattatgggg tttgctggcg acggcaaaac ctacacagca agcgaactgg
      241 ctattggtct ggtaatgctt atgcggcaac ggggaattga agctggtaac cgcccggtaa
      301 tgtttctgga cacagaaaca ggctctgact gggttaaacc ccgctttgat gcggaaaata
      361 tcgaattatt tacggcaaaa acccgctcgt ttgttgatct gcttgaagcc attaatgaag
      421 cggaaagcag cggttccgta atgatcatcg actccatcag tcacttctgg accggattat
      481 gcgacgagta tgccagacgc cgaaaccgta aacgaggact ggaattttca gactgggcgt
      541 ggctcaaaca ggagtggcga cgctttaccg atcgatttgt taacagtcag gcgcacatta
      601 ttatgtgcgg gcgtgcagga tatgagtatg actttttcga aggtgatgat ggcaaacgcc
      661 agttggcaaa aaccggtata aaaatgaaag cagaaacaga aaccggttat gaaccctcca
      721 tccggatcca gatggagaag caaatgaaca tagaaaccgg tcaggtgtgg cgcaccgcca
      781 gaatactaaa agatcgctcc acccgtattg atggacaggt attttcaaac ccaacattta
      841 aaaactttct tcctcatatc gagtctctta acctgggagg tgaacacccc ggcatcgata
      901 cctcacggga taacagtgaa ttatttgcca acgacggtac accgacatgg ctaaaagaaa
      961 agcgtgcaaa agaaatcgca cttgatgaaa ttatcgagtt gctcaacaag caccatgggg
     1021 gcaccagtaa cgacgccaaa cgcgcaaaag ctgatttgct ggaaaaaaca ttcacctccc
     1081 gctcatggga aagaatcaaa ggtatggact ggccgacaat caaagctggt cgtaatgcac
     1141 tatggattga actggaaggc gttgaatatg cattcccgga tccacaaaca caaaacgaaa
     1201 cgcaacaagc aggatacgat gagaatattc cggtgtaaaa ccaag
//
LOCUS       DAJTRW010000269         1219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRW010000269 DAJTRW010000000
VERSION     DAJTRW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>1219
                     /locus_tag="OO326_004257"
     CDS             <1..>1219
                     /locus_tag="OO326_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5968375.1"
                     /translation="KDDDHHFDARREKKFSRPDRKKIARYVTRTESHLEYLQSRGISP
                     EVVKRYEVVSGKVWNGERELDALVLPYKRDGELLQVKRISTERPDGKKVIMAEGDCEP
                     CLFGWQALDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNLD
                     RFEEIFISMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYI
                     GTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVH
                     GHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFE
                     DGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGD
                     HSEPLAWVIGSLLG"
BASE COUNT          312 a          264 c          356 g          287 t
ORIGIN      
        1 caaggatgac gatcaccatt tcgatgccag acgtgagaag aaattctcca gacctgatcg
       61 taagaaaatc gcccgctacg ttaccagaac agaatcccat cttgagtacc tgcaatcgcg
      121 tggcatatcg ccagaagtcg taaagcgcta cgaggttgtc agcggcaagg tgtggaatgg
      181 agaacgagaa ctggatgctc tggtgcttcc gtacaaacgc gatggtgagt tgttgcaggt
      241 caagcgaatc agcactgagc gcccggacgg gaagaaagtc attatggcag aaggtgattg
      301 cgaaccttgc ctgttcggat ggcaggctct ggacgctggc gtgagggtgg ttgtgctttg
      361 cgaaggcgaa attgattgta tgagctatgc gcaatacggc atctcggcgt tatccgtgcc
      421 gtttggtggc gggaaaggcg ctaagcaaca gtggattgag tttgagtatc acaacctcga
      481 caggtttgag gaaatattca tctcgatgga cgttgatgat gttggtcgtg aagccgcaag
      541 ggaaatcgta agccgactcg gtgaacatcg ttgccgtctt gttactctgc cgtacaaaga
      601 catcaacgaa tgcctgatga acggtgttac cgaggatgaa atctggcagt acatcggcac
      661 ggcatcctac ttcgatcctg aagaactcta cagtgcgcga gagttttacc aggacactat
      721 caacgctttc tacggcaagc agcagtatct gtttaatcca ccgtgggaat ctctggcaga
      781 taaattccag ttccgtgagg cagagttgac gctggtcaat ggtgtgcacg gtcacggaaa
      841 ggcatgccca ctgaatgagc ctattctttt agctgatggg acatggacta ctcacgggaa
      901 tgtaaaaatt ggcgatcagg tggcgtcagt agacggcaat ccgtcaactg tcactgggat
      961 attcccgcag ggtgttagag atgtttaccg agtcacattt gaagatggtc gttatgttga
     1021 ttgcgcaggc gatcacctat gggaggtcac tagtcgtgga ttcacgaaag gcgagaaacg
     1081 ccgcgtgata gataccttcg ggttgaagcg gttgagtgaa acgaagaggc acaaaaatgg
     1141 cgttaggatt cctgaaataa ctggtgactt tggcgaccac tcagagccat tagcatgggt
     1201 tatcggctcc cttctcggg
//
LOCUS       DAJTRW010000270         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRW010000270 DAJTRW010000000
VERSION     DAJTRW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            67..987
                     /locus_tag="OO326_004258"
     CDS             67..987
                     /locus_tag="OO326_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5968376.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            987..>1187
                     /locus_tag="OO326_004259"
     CDS             987..>1187
                     /locus_tag="OO326_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5968377.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYD"
BASE COUNT          338 a          275 c          285 g          289 t
ORIGIN      
        1 aatgatcaat atctatagtg atatcaccaa ccgtcaaaac gaaccgacaa ctccatgagg
       61 aactgaatga tgaataataa agtcagcttc actaacagca ataatccaac catctctttg
      121 tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc ggcaattgtg
      181 gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc cgaaaagctg
      241 gcggaaaagg gatttgtcac tattgcttat gacgcatctt atcaaggtga aagtggcggc
      301 gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc cgtgattgat
      361 tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc
      421 ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc catcggtacc
      481 gttagtgcgg tcaatattgg ttcgatgttc cgcaatggtt gggaaaacaa tgtgaaatct
      541 attgatgctc tgccacacat tgaagctggc tcaaacgccc gaaccagcga tattagcagc
      601 gaagaatacg ccattatgcc actggctcca atgaaggagg ccgatgcgcc gaacgaagaa
      661 ctgcgtcagg cctgggagta ttaccacaca cctcgtgcgc agtatccaac agcaccgggt
      721 tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca tatggcggaa
      781 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
      841 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
      901 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
      961 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
     1021 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
     1081 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
     1141 atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgac
//
LOCUS       DAJTRW010000271         1177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRW010000271 DAJTRW010000000
VERSION     DAJTRW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            261..>1177
                     /locus_tag="OO326_004260"
     CDS             261..>1177
                     /locus_tag="OO326_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5968378.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEE"
BASE COUNT          343 a          284 c          277 g          273 t
ORIGIN      
        1 ttcactgtag ttgatatcga cggtctatca ggaaacttta aaactcttgt tgaaggtgcc
       61 acaaaaatgt gcctagttta catcggaaat aatctgcccg cagaaaaaaa gccgaatttc
      121 tggcaaatct gattgcaatg aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca
      181 ctgtgcaggc ctgaatgaag aacctgggac atttattcat cactcgcagt aattttaatc
      241 tgagttgagg ttaaaaaaca atgaacaccg ataaacaagt ttacccactg tattacgaag
      301 caaaaaatga caaagtaaga aaacgtctcg gtattaaagg cggtttttac tgggctgaag
      361 cgaaaaaatt atccattgcc atctcccgtg gtgctgttgc gattgacgat gcaggctacg
      421 atgaagatga ctttaaaaaa cctgttcgcg tcaatttgcc cgttgttgat gacctcccgc
      481 cagaaggcgt atttgatacg gaattctgca accgttacga aaaaggcggg gaagatggca
      541 tcacaatggt atttatcgca tcttctccct ctgttcagga caaaccggcc agcactgaca
      601 ataccaacgt caacggcgaa gacatgacag aaattgagga gaacatgctc ctgccagttt
      661 ccggtcagga actgcccatt cgctggcttg cgcaacacgg cagcgaaaaa ccagtaacgc
      721 acgtttcacg cgacgaactc caggcattac acattgctcg ggatgaagaa ctaccagctg
      781 ttactgccct ggctgtttcc cacaaaacca gcctgctcga tccgctggaa attcgcgatc
      841 tccacaaact ggtgcgtgat actgacagag ttttccctaa tccaggcaat tcaagcctgg
      901 ggctgatgac tgcttttttc gaagcataca tggacgcaga ctacaccgat cgcggtctgc
      961 tgacaaaaga gtggatgaaa ggaaatcgtg tttcacgcat cacgcgcacg gcttccggcg
     1021 ctaatgctgg cggcgggaac ctcaccgatc gcggcgaagg tttcgttcac gatctgacgt
     1081 cactggcgcg cgacgtagcc actggcgtac tggctcgttc aatggacgtg gacatttata
     1141 accttcatcc ggcacacgct aaacgtgtcg aggaaat
//
LOCUS       DAJTRW010000272         1121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRW010000272 DAJTRW010000000
VERSION     DAJTRW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..720
                     /locus_tag="OO326_004261"
     CDS             <1..720
                     /locus_tag="OO326_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5968379.1"
                     /translation="IHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVNKPA
                     PSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFTGIC
                     YSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGVHNH
                     FGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRPLPA
                     DYVTHCQNGSVKIITPDSEDE"
     gene            complement(722..948)
                     /locus_tag="OO326_004262"
                     /pseudo
     CDS             complement(722..948)
                     /locus_tag="OO326_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          291 a          276 c          243 g          311 t
ORIGIN      
        1 atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat tcgtcgcgat
       61 ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat tgtaatggtg
      121 aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca tcgtgatgac
      181 ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat cttctttgat
      241 aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac cttcaccggc
      301 atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt aacagcaata
      361 ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag taactcttcg
      421 ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt gcataatcac
      481 tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt gatgaaccgt
      541 ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc ctctgccagc
      601 ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc ggcagattat
      661 gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga agacgaatga
      721 cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga ttacgttgca
      781 ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga cgctggtcat
      841 ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg cagagaatgt
      901 cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta cacccggatg
      961 gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc tgggttttta
     1021 atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc cagccggttt
     1081 gaattcatct cacaaccctg catagagcga atctcctgcc t
//
LOCUS       DAJTRW010000273         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRW010000273 DAJTRW010000000
VERSION     DAJTRW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            11..>1085
                     /locus_tag="OO326_004263"
     CDS             join(11..827,827..>1085)
                     /locus_tag="OO326_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5968380.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          249 a          279 c          341 g          216 t
ORIGIN      
        1 atccgtcctc atgttgtccg cttttactcc ccgcccgctg aaacgtctgt tcacggccaa
       61 ccagtgctgg acatccttcc tggatgcggg cggtctgcgc gatatcgagg ttgaggctgt
      121 cacaaaaatg ctggcctgcg gcacgcggat actgggcgtg aaggaataca tctgcgataa
      181 acctgagtgc ccccacgtca gatacgtcac taactcatgc ggcagccgtg cctgcccgtc
      241 ctgcggaaaa aaggccacag acctgtggat agcgacacag ctgaatcgtc ttcctgactg
      301 cgactgggta cacctggtct tcaccctgcc ggacacgcag tggccggtgt tcgaaagcaa
      361 ccggtggctg ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt atgccgcccg
      421 gaaacggggg caggaacccg gtatcttctg cgccatccac acgtatggcc gtcgtctcaa
      481 ctggcatccg catgtacatg tgtctgtaac ctgtggaggt ctgaataagc atggtcagtg
      541 gaaaaagctg agcttcctga aagacgcgat acgttcgcgg tggatgtgga atatgcggca
      601 acggcttctg aaagcgtggt cagaggggct ggcgatgccg gagtcgttgt cacatatcac
      661 gacggaatca cagaggagaa gcctggtgct gaaagccgga ggaaaatact ggcatgtgta
      721 catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc tacctgggtc gttatctgaa
      781 gaagccgccg atagcggcct cccggctggc acattacaac ggagggggag cctgagcttc
      841 cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca gcgtgagctg
      901 gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag gtacttcggg
      961 ttccttgcca accgggtgtg tggagagaag ctgccgcagg tgtaccgtgc tctggggatg
     1021 gataaaccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca gttcctgagt
     1081 cgtga
//
LOCUS       DAJTRW010000274         1081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRW010000274 DAJTRW010000000
VERSION     DAJTRW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO326_004264"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO326_004264"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5968381.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="OO326_004265"
     tRNA            complement(986..1062)
                     /locus_tag="OO326_004265"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          283 a          253 c          246 g          299 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 c
//
LOCUS       DAJTRW010000275         1005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRW010000275 DAJTRW010000000
VERSION     DAJTRW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..731)
                     /gene="virK"
                     /locus_tag="OO326_004266"
     CDS             complement(<1..731)
                     /gene="virK"
                     /locus_tag="OO326_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5968382.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFV"
     gene            complement(736..>1005)
                     /locus_tag="OO326_004267"
     CDS             complement(736..>1005)
                     /locus_tag="OO326_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5968383.1"
                     /translation="FGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAW
                     MCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          313 a          224 c          191 g          277 t
ORIGIN      
        1 acaaaggttt tgcgtttctg ataccagtat ctcaattgcc ggaaaacatg actgtgctca
       61 ctgactgcca gacactcaga gatattgcaa gctttcatca acgcacaaaa tgcttcaaaa
      121 ataatccgtt ttgggaaaat cccataacag gcccgggttg cgcaccgaat aacatcaggt
      181 ccggtatttt taggggctcc ctgtaatccg ccaataaaag caatgtgtcc attttgagtc
      241 agaatgactg aaaatgttaa ccgggcaata actgttttat tgtaaaacaa tgacaacatc
      301 aactctcctt cccggtcaaa accactggga gaacactgga tatcaaaatt ctctccatct
      361 ttcccgatga attggactaa tgataccgat ttttgtggaa acaaaaattt agaatatttt
      421 tctggcaaat tttgcacaaa gcgataatgc tcaagaatat attgtccacg ggtccaggcg
      481 cgcccccctt tatgcaaata agggcgatga attcttgctg gcaacgtggg gtgtattgcc
      541 agaatatcct caatgcacgg caaggaacgc agttcataat aatatcgaat actgacaacc
      601 ggatttatag aacatcgcaa aatgtacatg aaccgaaatt ttctgtgttc ccacttcccc
      661 ggtagtgaat ctgaggaaaa aataatcctt ttaaggaaac cgataaatga taagttactt
      721 atagaaaaca tattgttact tttgcttcgc taatgtgagt atttttaacg cagttttatt
      781 aatatcaaac cgctcttcta tatcctgttt tgccaggcga gccatctgat aaaaacgccc
      841 agggtcatta aaaaaatcat tcagggcgca catccatgcg tgcttattac ttgctggtaa
      901 caatgtcccg gtctggttgt tctgaataac ctcagggatt ccaccaatct gagtggccac
      961 tacaggcaca gaaaatgccg atgcttctgc cagcaccata ccaaa
//
LOCUS       DAJTRW010000276          973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRW010000276 DAJTRW010000000
VERSION     DAJTRW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            417..>973
                     /locus_tag="OO326_004268"
     CDS             417..>973
                     /locus_tag="OO326_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968384.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          347 a          189 c          157 g          280 t
ORIGIN      
        1 caaagccaac ggtattctga tttctgaagt taatttcaga ctcgttcaag ctccattttc
       61 aaaaaaaata tatcagggtt tgttactaat aacttctgaa aacagaaaaa aatagagcag
      121 taattctatt tcagagagga gtaactccat ctatcctcga tacgtactga acgttatcgt
      181 gatatattca tcatgctttc ttcaccatta ttcgagtata gggagagacg caatgacctt
      241 aaataccttt ttatggctga attaccattc tcaggaataa cagcagactc cttcccatag
      301 acggcttagg gggaaagcaa tacccggaga aagggtacaa agcaaactgt tttaaaatta
      361 ataagccata ttctatttaa ctagaattct tatcgttgat aagaattcta gttataatga
      421 ttaaatcaac caatatacag gcaatcggtt ctggcattat gcatcaaata aacaatgtat
      481 actcgttaac tccattatct ttacctatgg aactgactcc atcttgtaat gaattttatt
      541 taaaaacctg gagcgaatgg gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct
      601 tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta tctgagttag
      661 atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata agaaaaaacc
      721 tattaacaca tctccctgat ttaccaccaa tgcttaaggt aatacatgct caatttaatc
      781 aactggaaag cttacctgcc ttacccgaga cgttagaaga gcttaatgcg ggtgataaca
      841 agataaaaga attaccattt cttcctgaaa atctaactca tttacgggtt cataataacc
      901 gattgcatat tctgccacta ttgccaccgg aactaaaatt actggtagtt tctggaaaca
      961 gattagacag cat
//
LOCUS       DAJTRW010000277          965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRW010000277 DAJTRW010000000
VERSION     DAJTRW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..584)
                     /gene="arsB"
                     /locus_tag="OO326_004269"
     CDS             complement(<1..584)
                     /gene="arsB"
                     /locus_tag="OO326_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5968385.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVML"
     gene            complement(638..>965)
                     /gene="arsR"
                     /locus_tag="OO326_004270"
     CDS             complement(638..>965)
                     /gene="arsR"
                     /locus_tag="OO326_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5968386.1"
                     /translation="FKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLAL
                     LRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSGD
                     SKNICS"
BASE COUNT          264 a          280 c          236 g          185 t
ORIGIN      
        1 tgtagcatca ccagcgtggc aacaatcgcg gcgatatcca ccggcaccat caccgaggcg
       61 tattcgcgaa agccgaggcc aaagaaatca gcggaaacga tattcaccag gttggagaca
      121 ataagcggca ggctggcggt atcggcaatg aatccggccg ccatcacgaa cgccagcgtc
      181 gtgcctttac tgaatcctaa agccagcagc atggcgatga caatcggcgt caaaataagc
      241 gccgcgccat cattggcaaa cagggccgca acggcggcac cgagcaggac aatccaggta
      301 aacagcaagc gaccgcgtcc attgccccag cgtgaaacgt gcagcgctgc ccattcaaaa
      361 aagccggact catccagcag caggctgatg ataatgacgg caataaacgc cgccgtcgcg
      421 ttccagacga tattccacac caccggaata tcacccggat gaaccacgcc cgtaaccaac
      481 gccagtaccg cgccgagcgt tgcactccag ccgatgccta aacctttcgg ctgccagata
      541 accaatacga tggtcaggac aaagatagcg cctgccagta acataatgcc tcccggataa
      601 aacacatgtg caaatccata tgtgtttagc taaattttta actgcaaatg ttcttactgt
      661 ctccggaaca gttttgtctg gccaggttgc ggacaattgc ctgaatcttt tcctgttcac
      721 atcgccaggc ttcatcaata attttcgccg cccatgctgg aatatgcggc gataagcggt
      781 aatgaaccca cttaccttgc ttgcggtcca gcaatagccc gctttcacgc agcaatgcca
      841 gatggcggga tatcttgggc tgcgactgat cgagggcagt gcagagatcg cagacgcata
      901 actctcccag ttcgctaagc agtaaaacga tgcccagacg ggtttcatca gcaagaattt
      961 tgaac
//
LOCUS       DAJTRW010000278          955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRW010000278 DAJTRW010000000
VERSION     DAJTRW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(108..680)
                     /locus_tag="OO326_004271"
     CDS             complement(108..680)
                     /locus_tag="OO326_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5968387.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          304 a          198 c          177 g          276 t
ORIGIN      
        1 aaataactta cattaaactg ttgttttaaa gagcctgatt aaacaacttt ttctggacat
       61 aacgactccc tggccaaagc caactgaata aaaaggccct tgataaatta tttcttatat
      121 tcgacagtaa attgtgaatc agcgttcgcc gtaccagccg ttacggtcgc attggttgcc
      181 acatagcgtg caataaactt catttcagcc ttgccttcag catcgatagc ggtataagca
      241 gagtcgttat aaagtttaac ctgcgaatta tcggactggt taaaaatacg aataccaacg
      301 ccggttgccg caatcgcttg tcctgtaccg gtaaaatcac cagggttgcc tgctgtaagc
      361 ggcgtaccca ctttcaggtc gccgtcgccg ccaggcgctt ctgtaccatc aaaacgaacg
      421 gctgcttgag tgtacgttgc cgggcaattt tcaagtttaa tactgaactt ctgtggcgac
      481 gccagactac ctacacctga aaatgcattt ttactgactt tacccagtgg gacaatttga
      541 tcggctgtat cggaggtaac ttcacaagta gtgtcgacaa tttcaccgac gaagttaaca
      601 acaccatctt ctgccagtgc atgcccggcc agcattagag atgccagagt gaatgcaccg
      661 cctataatat tacgcttcat aaaaactcca ttgatataat atatccttta ccatgaatca
      721 tcacttatat taataagtgt gtttttgatt caaaggaata gtacaccaaa gagacggctt
      781 tatctaaaaa taatattaat tacgcaaaaa taaaatatag atttattgtt taaatattat
      841 ttaaacagta aaaaatcaag tttgttatca gacattaatg ataattcaga atatatttat
      901 atagaaagat agatgaattt attgatgtca gttataaaaa agggccaccg gtcgg
//
LOCUS       DAJTRW010000279          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRW010000279 DAJTRW010000000
VERSION     DAJTRW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            17..895
                     /locus_tag="OO326_004272"
                     /pseudo
     CDS             17..895
                     /locus_tag="OO326_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          229 a          224 c          242 g          200 t
ORIGIN      
        1 ttaaagagag gtgcccatga gcggtaagcg ttatcccgaa gagtttaaaa ctgaagcagt
       61 caaacaggtt gttgatcgcg gttattctgt tgccagcgtt gcaacacgtc tcgatatcac
      121 cacccacagc ctttatgcct ggataaagaa gtacggtccg gattcttcca ctaataaaga
      181 acagtcagat gctcaggccg agatccgccg tctccagaaa gagctgaaac gggttaccga
      241 cgaacgggac atattaaaaa aagccgcggc gtacttcgca aagctgtccg actgaggtac
      301 gcctttatcc gtgacaactc ctgttgctgg cctgttcgcc tgctctgtcg ggtgctggat
      361 gttcatccca gtggttttta cgcctggctt cagcagccgc attcacaacg ccatcaggca
      421 gacctgagac tgacaggaca gattaaacag ttctggctgg aatcgggatg cgtctatggt
      481 tatcgcaaaa tccatctgga tctgcgtgac agcgggcaac agtgcggagt aaacagagtc
      541 tggagactga tgaaacgtgt cggaataaag gctcaggtcg gataccgaag cccgcgggca
      601 cgtaaaggcg aggccagtat cgtgtcaccc aacaggctcc agcgacagtt caatccggat
      661 gctcctgatg agcgttgggt aacggacata acctacatca ggacccacga aggctggctg
      721 tatcttgccg ttgttgttga tctgttctca cgcaaaatta tcggctggtc catgcaatcc
      781 cggatgacaa aggacattgt cctgaacgca ctgctgatgg ctgtatggcg gcgtaatccc
      841 gaaaaacagg tgctggttca ttcggatcag ggcagtcagt acacaagcca tgagt
//
LOCUS       DAJTRW010000280          888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRW010000280 DAJTRW010000000
VERSION     DAJTRW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(182..>888)
                     /locus_tag="OO326_004273"
     CDS             complement(182..>888)
                     /locus_tag="OO326_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5968388.1"
                     /translation="KRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNAGQDCTAAC
                     RIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKAVEEAKATG
                     HIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNEEQVVNWAN
                     DSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLSGYGKDMSL
                     YGLEDYTVVRHVMVKH"
BASE COUNT          229 a          259 c          169 g          231 t
ORIGIN      
        1 gtttaataac gatactctaa agccttatgc taatcaactg tgacaggatg aaaaatcgcc
       61 atcaaacatt tagcctaatt tttacgccag ttattaatgt aatcattcta atacttaacc
      121 gccattaaac tcagaaccta aaacgatagt ggccgtccta taacagccac taacatctaa
      181 cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca taaagtgaca
      241 tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg catttcactt accagcatga
      301 aatgggtatt gacccaggta caaccatatt gcagccgtgc gctgacgcga tgcgccctgc
      361 ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc gcccagttca
      421 ccacctgttc ttcgttgtcg aagggcgtaa cactcactac tggaccaaat acctcttttt
      481 gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag taatagccat
      541 tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct ttcgcctctt
      601 ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca agctccgtag
      661 actcgtcatc cggcgcacca gattttaacg ttgccaccgc agcacccagt ttttccacca
      721 gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc cgcagtacaa tcctgtccag
      781 cattgtaata gccaaatgta cgtacacctt cgaccactgc ttcaatatcc gcatcatcaa
      841 aaacaatcac tggcgctttg ccaccaagtt ccatatgagt acgcttaa
//
LOCUS       DAJTRW010000281          887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRW010000281 DAJTRW010000000
VERSION     DAJTRW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(40..180)
                     /locus_tag="OO326_004274"
     CDS             complement(40..180)
                     /locus_tag="OO326_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968389.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            319..>887
                     /gene="queE"
                     /locus_tag="OO326_004275"
     CDS             319..>887
                     /gene="queE"
                     /locus_tag="OO326_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5968390.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQP"
BASE COUNT          250 a          182 c          212 g          243 t
ORIGIN      
        1 gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga taaataaatt
       61 gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag aaaaaataaa
      121 tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt cttctgacat
      181 aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta aatctgcatg
      241 taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt aacaattatc
      301 cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac cctgcaaggt
      361 gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg cccggttggc
      421 tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga agtctccctt
      481 ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag cagtgaagac
      541 ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat tacgggtggt
      601 gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa cggttttagc
      661 tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac ctgggttacc
      721 gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc actggagcga
      781 gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact ggatgaacta
      841 ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagcc
//
LOCUS       DAJTRW010000282          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRW010000282 DAJTRW010000000
VERSION     DAJTRW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..109
                     /locus_tag="OO326_004276"
     CDS             <1..109
                     /locus_tag="OO326_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5968391.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            447..840
                     /locus_tag="OO326_004277"
                     /pseudo
     CDS             447..840
                     /locus_tag="OO326_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          252 a          177 c          204 g          207 t
ORIGIN      
        1 aaatagtggg gacactatgc ttgacaatgc aatgaaaagc aaagattcaa aaatgattga
       61 ttttttatta aaaaatggag caatattagg caaacgattt gagatatgac ctaaaagctc
      121 ggccaaataa taatgtcgtc gattatcgaa gtcatcatga gtgattctgt ccccacagtg
      181 cagcatcagc atgacagtga attgtctggc gtggtccatt taatgagatg gagatggtcc
      241 ctctttccta aatagctgtg cctaataccg ctacactttt gccagcccat gtttgcctcc
      301 ggggaatggg ctgacggttc cttcaaaatc cattgcacgc agttatgtcg gagcgggaac
      361 ttgactcctg tctttacggg agtgaggtag cctgagttta acggacactc cttcctgaaa
      421 tagaatggca tcagaaggag ccaataatga gcagaaaaac ccaacgttac tctacggagt
      481 ttaaagccga agccgtcaaa acggttcctg aaaatcagct ttcgatcagt gaaggagctt
      541 cccgattatc tgttcctgaa ggcactttag gacaatgggt taccgccgca agaaaagggc
      601 tcaatacacc aggctcccgc acagtggctg agctggaatc tgaagttatg caactgcgta
      661 aggctttaaa tgaagcacga cttgagcgag atatattaaa aaaagcaaca gcgtattttg
      721 cacaggagtc gctgaaaaat acgcgttagt cgaacaatgg cgacaacaat tccctgttga
      781 agcgatgtgt cgggtacctg gtgtatccag gagcggttat tacgaccggg tacagcatgc
//
LOCUS       DAJTRW010000283          787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRW010000283 DAJTRW010000000
VERSION     DAJTRW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..408
                     /locus_tag="OO326_004278"
     CDS             <1..408
                     /locus_tag="OO326_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5968392.1"
                     /translation="DTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITA
                     PGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACR
                     IYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            432..>787
                     /locus_tag="OO326_004279"
     CDS             432..>787
                     /locus_tag="OO326_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5968393.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          208 a          177 c          224 g          178 t
ORIGIN      
        1 gacacaataa aaatagctta caccggagat ggtcgcgtgg tgtgggtcgg taaggatgtg
       61 acaggcatcg agccacgcaa tgccagcgtt atcgaagttc cggatattac cgctaaccga
      121 cggattaccg cgccgggtta ctggttttac cgcaatgatg aatttgtctt tgactacaga
      181 ctcaaagcgg aagatgagcg tgatgccctt ctggctcagg tcagtgcccg gacaggggaa
      241 tgggaagaag acctgctgct ggggttaatc agcgacgaag ataaagaaaa gctgaaagcc
      301 tgtcgtattt acgcgaaatc gctgcaggcg atggatttca gcaccatcac tgataaatcc
      361 tcatacaacg ccattgaatg gcccgcctct ccggaaggtt cttcctgatt taatttatcg
      421 cgagaaaaac aatgtctgta gtgatatcag gtgcgctgac tgatggcgca ggtatcccca
      481 tgtccggata ccatattatt ctgaaatccc gggtaaaccc cccggaagtg gtgatgaaca
      541 ctgttgctga tgtgatgaca ggaaacgatg gtgaatactg tttccatgcg cggactggaa
      601 aatatggtgt gtatctgaaa caggactggc gcaacgagta caacgttggc gacattgctg
      661 tatatgagga ctcaaagccc ggcacgctga atgactttct gattgctcct gatgagggcg
      721 acctgaaacc ggatgtcgtc aaacgctttg aggaaatggt ggcgcaggcg cagcagagcg
      781 ccggggc
//
LOCUS       DAJTRW010000284          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRW010000284 DAJTRW010000000
VERSION     DAJTRW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            459..>763
                     /locus_tag="OO326_004280"
     CDS             459..>763
                     /locus_tag="OO326_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5968394.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          259 a          152 c          127 g          225 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJTRW010000285          762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRW010000285 DAJTRW010000000
VERSION     DAJTRW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..762
                     /locus_tag="OO326_004281"
                     /pseudo
     CDS             1..762
                     /locus_tag="OO326_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          198 a          197 c          179 g          188 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat atgcattatt
      721 gctgctcaat agcatgccgg aaaaaacagc tgagcattac cg
//
LOCUS       DAJTRW010000286          757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRW010000286 DAJTRW010000000
VERSION     DAJTRW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            121..>757
                     /locus_tag="OO326_004282"
     CDS             121..>757
                     /locus_tag="OO326_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5968395.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAA"
BASE COUNT          157 a          206 c          227 g          167 t
ORIGIN      
        1 aatcgcccgc tgcacggagc gggaatttgc gaccattctt gccggtatca gcgccggcgg
       61 tatgtactgc ccggagtgtg gcacggttca ctggccggac ggagtcgccc cgcccttctg
      121 atgctccccc gttttgccga tatttttcag cagggaaacc gctggcttaa ctggctggag
      181 aaacaaccgg aaggggcagt ccgtccggtg gtaattgaat cagtgacaaa aatcatggcc
      241 tgcgggacca cactgatggg ctacacgcag tggtgttgtt catcaccgga ctgttgccat
      301 acaaaaaagg tctgcttccg gtgtaaaagc cgctcctgcc cgcactgcgg ggtgaaggct
      361 ggcgcacagt ggatacagta tctgctgagc ctggtcccct actgcccgtg gcagcatatt
      421 gtgttcacac ttccctgcca gtactggtcc ctggtgttcc acaaccggtg gttactggca
      481 gagatgagcc gcattgcagc ggatgtgata ctggaaatct gccatcaggc agatgtggag
      541 ccggggatat tcacggtgat ccacacatgg gggcgtgacc agcagtggca tccgcatatc
      601 catttatcga caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt
      661 tacgcccgta aggtgatgag catgtggcgc taccggataa cacggttgct gtcacggaaa
      721 taccctgacc tggtgatgcc ggatgcgctg gcagcag
//
LOCUS       DAJTRW010000287          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRW010000287 DAJTRW010000000
VERSION     DAJTRW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>756
                     /locus_tag="OO326_004283"
     CDS             <1..>756
                     /locus_tag="OO326_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5968396.1"
                     /translation="AIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTW
                     VSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAF
                     VGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPF
                     AISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSN
                     GIPKKGLLLAAVKMTALMILITLMNSAGGKASD"
BASE COUNT          144 a          179 c          199 g          234 t
ORIGIN      
        1 tggcgattgg tattaccgct gtatcttatc tttccacctt cttcccagta ttaaatgatc
       61 ctgttccggc gggtatcgcc tgtattgcta tcgtctgggt atttaccttt gtaaatatgc
      121 tcggcggtac ctgggtaagc cgtttaacca ctattggtct ggtgctggtt cttattcctg
      181 tggtgatgac tgctattgtt ggctggcatt ggtttgatgc ggcaacttat gcagctaact
      241 ggaatactgc ggataccact gatggtcatg cgatcattaa aagtattctg ctctgcctgt
      301 gggccttcgt gggtgttgaa tccgcagcag taagtactgg tatggttaaa aacccgaaac
      361 gtaccgttcc gctggcaacc atgctgggta ctggtttagc aggtattgtt tatatcgctg
      421 cgactcaggt gctttccggt atgtatccgt cttctgtaat ggcggcttcc ggtgctccgt
      481 ttgcaatcag tgcttcaact atcctcggta actgggctgc gccgctggtt tctgcattca
      541 ccgcctttgc gtgcctgact tctctgggct cctggatgat gttggtaggc caggcaggtg
      601 tacgtgccgc taacgacggt aacttcccga aagtttatgg tgaagtcgac agcaacggta
      661 ttccgaaaaa aggtctgctg ctggctgcag tgaaaatgac tgccctgatg atcctcatca
      721 ctctgatgaa ctctgccggt ggtaaagcat ctgacc
//
LOCUS       DAJTRW010000288          749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRW010000288 DAJTRW010000000
VERSION     DAJTRW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            53..274
                     /gene="kil"
                     /locus_tag="OO326_004284"
     CDS             53..274
                     /gene="kil"
                     /locus_tag="OO326_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5968397.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            268..444
                     /locus_tag="OO326_004285"
     CDS             268..444
                     /locus_tag="OO326_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968398.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            519..>749
                     /locus_tag="OO326_004286"
     CDS             519..>749
                     /locus_tag="OO326_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968399.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAE"
BASE COUNT          263 a          138 c          151 g          197 t
ORIGIN      
        1 aaataacttt cctgagattt cctttgtaag actgtttatt aaggaggctt ccatgattgc
       61 tcatcacttc ggaactgatg aaataccacg tcagtgtgtg actcctggcg attatattct
      121 tcatgaaggt cggacatata tcgcctcggc aaacaatatt aaaaagcgaa aactttatat
      181 tcgtagcctg actacaaaaa catgcatttc tgactgcatg attaaagtct tcctcggtcg
      241 tgatggttta cctgtaaagg cggagtcatg gtaatgacta agaaaataaa atgtgcttat
      301 cacctttgca ataaagaaat tgaagaaagc aaaatcatta caagaccact tcatttcatg
      361 cgtggagtta taccaacgac ggaaatgaaa aaatattgta gtgaaatctg tgccgaaaaa
      421 gaccagatgg cacacgaact ttaattaact gactatccga aactgaattt atgccagcaa
      481 tggcagggat tcgctcaacc ttaattaagg agaaaaacat gattaccagt tatgaagcca
      541 ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag cgtattggct
      601 acgtgattaa gacagaaaat aaagaaaccc cattcactgt ggttgatatc gacggtccat
      661 caggcaacgt taaaacactt aacgatggtg taaaaaaaat gtgtctggtg cacataggaa
      721 agaatctgcc cgcagaaaaa aaagccgaa
//
LOCUS       DAJTRW010000289          742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRW010000289 DAJTRW010000000
VERSION     DAJTRW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..237
                     /locus_tag="OO326_004287"
     CDS             <1..237
                     /locus_tag="OO326_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5968400.1"
                     /translation="DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAP
                     SVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(280..480)
                     /gene="glgS"
                     /locus_tag="OO326_004288"
     CDS             complement(280..480)
                     /gene="glgS"
                     /locus_tag="OO326_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5968401.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          229 a          169 c          132 g          212 t
ORIGIN      
        1 gatattttga gtgattatag ccatctggat atgggtaatg gactgctatt gaagatattt
       61 cataaagatg gaacagcaac ggaatttaac cgcttctcac aatttgcttc tttttcatcg
      121 tctagtgcac cttcggtcac cgcaccattc agggcagaac ttagtgcgaa cccggcagag
      181 actgttgttg agggaccgtt tagtaaagac gtaatcctca aaatcaccta taactagcat
      241 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
      301 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aagttctatg
      361 ttcttcatcc atgttaccag taacagccag aatatcgacc gggcgacctt ctgcgtgcat
      421 tctggcaaaa ctacgcgcca ggaaatcgaa attattcaaa gaattaagac tatgctccat
      481 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      541 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      601 actttttagt gaaaaataaa aaaccatatt aaaatacttt ctcttccaga acttctctaa
      661 catgtgttat tattaacaaa gacaaatgta agcagggaaa ctattccaag gtaaatattg
      721 cgtcactaaa tggacattgg ag
//
LOCUS       DAJTRW010000290          736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRW010000290 DAJTRW010000000
VERSION     DAJTRW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>736
                     /locus_tag="OO326_004289"
     CDS             <1..>736
                     /locus_tag="OO326_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5968402.1"
                     /translation="SPKGVIQFQYRYRWHGKPNRLGLGRYPSLSLKDARQITADLRKL
                     YFSGTDPRTYFEEKVENSMTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFP
                     NRPASSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPR
                     DFGSRAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTE
                     FDLLDNVWTVPKEHSKMGNVIRRPIFE"
BASE COUNT          193 a          182 c          190 g          171 t
ORIGIN      
        1 tcaccgaagg gggtcatcca gttccagtac cgctatcgct ggcatggcaa gcctaatcga
       61 cttgggcttg gtcgataccc atccctgtct ttgaaggatg ccagacagat cactgctgac
      121 ttgcgaaagc tctatttctc aggaacggat ccacgcacct attttgaaga gaaggtggag
      181 aactccatga cggtcgccca gtgtctcgac tactggttcg acaactacgt ctctacaact
      241 ctcagagaaa agacccaggc actttatcga tcaacggtta tgaagcgcat gcatgacgcc
      301 ttccctaatc gtccggcatc ttctatcacg gttaagcaat gggttgacct gcttaccgaa
      361 gaagaaaaag ataatccacg ccgagcaagg caggtgctaa gtcaactaag atcagcaatt
      421 agttggtgca tgcggcgtca gttgatagat agttgcgcaa ttatgagcat ccagccaagg
      481 gacttcggct cccgcgctga ggtaggggat cgggtactgt cgtatcacga actggctaag
      541 atttggcttg ctattgaaag aagccgtgcg tctacgtcaa ataagctcct tcatcagatg
      601 cttatgctgt ggggggcgag gctctcagag cttaggctgg caacaaagac agaatttgac
      661 ctgctggaca acgtatggac cgtaccgaaa gagcatagca agatgggtaa tgttatccgc
      721 cgtccaatct tcgaac
//
LOCUS       DAJTRW010000291          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRW010000291 DAJTRW010000000
VERSION     DAJTRW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            35..>723
                     /locus_tag="OO326_004290"
     CDS             35..>723
                     /locus_tag="OO326_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5968403.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          179 a          171 c          226 g          147 t
ORIGIN      
        1 tgcgatcccg ccaggcttca gggggtaaca gaccatgaaa aatattgcga caggaggcgt
       61 tctggagcgt atccgcagac tgaccccacc acatgtaacc gccccattca gaacggttgc
      121 ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt tgcgaggaga tcaaccgcct
      181 gaatcgtcag ttgcgggtgg aaaaaatcct gaaccgctcc ggcatccagc cgttgcaccg
      241 gaagtgttcg tttgcgaatt accgggcgca gaacgacggt cagcgacatg cactgagtca
      301 ggcgaaatcc atagctgacg aactgatgac aggctgtacg aattttgtgt tcagcggtaa
      361 gcccggcacc ggaaaaaatc atcttgcagc cgcgattggc aaccggctga tggcgaaggg
      421 gcgtagcgtg attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg agagctacga
      481 caacggcaaa tccggtgaaa aatttttaca ggagctttgt ggtgttgacc tgctggtcct
      541 ggatgaaatt ggcatgcagc gggatacgaa aaacgagcag gtggtactga accagattgt
      601 tgatcgccgg acggcatcgt tacgcggtgt ggggatgctg acaaatatta accatgcagc
      661 gatgaataca cttctcggcg agcgggtgat ggatcgcatg gtcatgaacg gcgggcgctg
      721 ggt
//
LOCUS       DAJTRW010000292          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRW010000292 DAJTRW010000000
VERSION     DAJTRW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..684
                     /locus_tag="OO326_004291"
     rRNA            <1..684
                     /locus_tag="OO326_004291"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          178 a          167 c          210 g          152 t
ORIGIN      
        1 gagctaacgc gttaagtcga ccgcctgggg agtacggccg caaggttaaa actcaaatga
       61 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgatgca acgcgaagaa
      121 ccttacctgg tcttgacatc cacggaagtt ttcagagatg agaatgtgcc ttcgggaacc
      181 gtgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt gaaatgttgg gttaagtccc
      241 gcaacgagcg caacccttat cctttgttgc cagcggtccg gccgggaact caaaggagac
      301 tgccagtgat aaactggagg aaggtgggga tgacgtcaag tcatcatggc ccttacgacc
      361 agggctacac acgtgctaca atggcgcata caaagagaag cgacctcgcg agagcaagcg
      421 gacctcataa agtgcgtcgt agtccggatt ggagtctgca actcgactcc atgaagtcgg
      481 aatcgctagt aatcgtggat cagaatgcca cggtgaatac gttcccgggc cttgtacaca
      541 ccgcccgtca caccatggga gtgggttgca aaagaagtag gtagcttaac cttcgggagg
      601 gcgcttacca ctttgtgatt catgactggg gtgaagtcgt aacaaggtaa ccgtagggga
      661 acctgcggtt ggatcacctc cttaccttaa agaagcgttc tttgtag
//
LOCUS       DAJTRW010000293          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRW010000293 DAJTRW010000000
VERSION     DAJTRW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            155..340
                     /locus_tag="OO326_004292"
                     /pseudo
     CDS             155..340
                     /locus_tag="OO326_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..>693)
                     /locus_tag="OO326_004293"
     CDS             complement(440..>693)
                     /locus_tag="OO326_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5968404.1"
                     /translation="RKNIKLLTKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGY
                     KALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          197 a          170 c          160 g          166 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gccttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt cagtaatttt atgtttttcc tga
//
LOCUS       DAJTRW010000294          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRW010000294 DAJTRW010000000
VERSION     DAJTRW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>682
                     /locus_tag="OO326_004294"
     CDS             <1..>682
                     /locus_tag="OO326_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5968405.1"
                     /translation="AAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFSKDA
                     NQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAMKKG
                     ALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGL
                     QDFTRSLTN"
BASE COUNT          215 a          123 c          163 g          181 t
ORIGIN      
        1 gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc cgcttatcag
       61 cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga tttcgtattt
      121 tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac aacgggttta
      181 gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag agagtctcta
      241 ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg tctgatgggg
      301 cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc tggtaagggt
      361 cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg tatggttcag
      421 gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat gaagaagggg
      481 gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa acaaatcacc
      541 gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg gaacttgaaa
      601 aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg tctgcaagat
      661 tttacaagaa gcttaaccaa tc
//
LOCUS       DAJTRW010000295          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRW010000295 DAJTRW010000000
VERSION     DAJTRW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(40..115)
                     /locus_tag="OO326_004295"
     tRNA            complement(40..115)
                     /locus_tag="OO326_004295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Ile,seq:cat)
     gene            complement(292..>682)
                     /locus_tag="OO326_004296"
     CDS             complement(292..>682)
                     /locus_tag="OO326_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5968406.1"
                     /translation="YKAWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQE
                     RLKKLIWLAAQAVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLD
                     SEALLFVMRTRSEQKAAFSKQSIAKVD"
BASE COUNT          171 a          175 c          135 g          201 t
ORIGIN      
        1 tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg
       61 aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg
      121 cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac
      181 gtcctgtgcc atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac
      241 catcgcgtac aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac
      301 ttttgcaata ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa
      361 caataaagcc tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt
      421 aaatgttttg gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc
      481 ataaccttcc ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt
      541 tttcaggcgt tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc
      601 ttcccacgcc cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca
      661 ccacaacaac caggctttat ag
//
LOCUS       DAJTRW010000296          667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRW010000296 DAJTRW010000000
VERSION     DAJTRW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            59..556
                     /locus_tag="OO326_004297"
                     /pseudo
     CDS             59..556
                     /locus_tag="OO326_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          167 a          166 c          199 g          135 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttccc
//
LOCUS       DAJTRW010000297          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRW010000297 DAJTRW010000000
VERSION     DAJTRW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            103..>659
                     /locus_tag="OO326_004298"
     CDS             103..>659
                     /locus_tag="OO326_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968407.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTRW010000298          652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRW010000298 DAJTRW010000000
VERSION     DAJTRW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            21..>652
                     /gene="ospC4"
                     /locus_tag="OO326_004299"
     CDS             21..>652
                     /gene="ospC4"
                     /locus_tag="OO326_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5968408.1"
                     /translation="MTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGK
                     TDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYH
                     IPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIA
                     LYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFIN"
BASE COUNT          242 a           85 c          116 g          209 t
ORIGIN      
        1 atgactctcc atatggatat atgacattaa ccgatcactt tgataatgct attccacctg
       61 ttttttacca tgagcaccaa tcatttttct tagataactt taaagaggtt gtggatgaag
      121 ttagcaggta tgtgcatggt aatcagggga aaactgatgt tccaatattt aacactaaag
      181 atatgaggct tggaattggg ttgcatctta ttgattttat aagaaagagt aaagatcaag
      241 gattcagaga gttttgttat aacaaaaata ttgatcctgt tagtctggat agaattataa
      301 actttgtgtt tcagcttgag tatcatatac caagaatgct aagtacagat aacttcaaaa
      361 aaataaaact cagagacata tctttggagg atgcgattaa agcatctaat tatgaagaga
      421 ttaacaataa agtgactgat aaaaaaatgg ctcatcaggc ccttgcttat tctttaggca
      481 ataaaaaagc tgatatagca ctttatttac tatctaaatt taattttaca aaacaagatg
      541 tcgcagagat ggagaaaatg aaaaacaata gatattgtaa tctgtatgat gttgaatact
      601 tacttagtaa agatggtgcc aactataaag tgctagaata ttttatcaat aa
//
LOCUS       DAJTRW010000299          651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRW010000299 DAJTRW010000000
VERSION     DAJTRW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            18..651
                     /locus_tag="OO326_004300"
                     /pseudo
     CDS             18..651
                     /locus_tag="OO326_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          195 a          162 c          165 g          129 t
ORIGIN      
        1 acaacacagg tgctccaatg aaaaaaagaa attttagcgc agagtttaaa cgcgaatccg
       61 ctcaactggt tgttgaccag aaatacacgg tggcagatgc cgccaaagct atggatgttg
      121 gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac
      181 caaaagcctc ccccattacc ccggaacaaa ttgaaatccg tgagctcagg aaaaagctac
      241 aacgcattga aatggagaat gaaatattaa aaaaggctac cgcgctcttg atgtcagact
      301 ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg tcacactctg
      361 ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc ctgaaaaacc
      421 agacggcaga cgggctgtat tacgcagtca ggtacttgag ctacatggca tcagccacgg
      481 ttcggccgga gcaagaagca tcgccacaat ggcaacccgg agaggctacc agatgggacg
      541 ctggcttgct ggcaggctca tgaaagagct ggggctggtc agctgtcagc agccgactca
      601 ccggtataaa cgtggtggtc atgaacatgt tgctatccct aactaccttg a
//
LOCUS       DAJTRW010000300          646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRW010000300 DAJTRW010000000
VERSION     DAJTRW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..>646)
                     /gene="arsB"
                     /locus_tag="OO326_004301"
     CDS             complement(<1..>646)
                     /gene="arsB"
                     /locus_tag="OO326_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5968409.1"
                     /translation="LKSPAEAIKDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAV
                     GALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVL
                     ADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCD
                     LGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAALALRL"
BASE COUNT          179 a          181 c          166 g          120 t
ORIGIN      
        1 agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat gataatccct
       61 gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac gtgcagccag
      121 agcagcgttg ccaggctacc aatcggggta attttcggcc ccaaatcgca gccaatcacg
      181 ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc aatggacaac
      241 gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc ggtgaggaat
      301 ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag tacgccagaa
      361 agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat gccgagcgag
      421 aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat cgcatgaccg
      481 cgtttagcga cgacaaataa tatcagtgcg cccacagctg caatggcgct caccggaatg
      541 ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac ccagccagtt
      601 ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagca
//
LOCUS       DAJTRW010000301          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRW010000301 DAJTRW010000000
VERSION     DAJTRW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(6..>637)
                     /locus_tag="OO326_004302"
     CDS             complement(6..>637)
                     /locus_tag="OO326_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5968410.1"
                     /translation="NHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKA
                     TRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKK
                     LNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSEC
                     FYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
BASE COUNT          171 a          143 c          123 g          200 t
ORIGIN      
        1 gacagttata cacgaggcat ataggtctga ttccgatatt caattggagt cagacctttt
       61 aatttcaggc taattcttct gctgttatag tattcaatat attccgtaac agcatccttc
      121 agttcgctta tattactgaa ctcatcaaga taaaaacact ccgactttaa ggttccaaag
      181 aaacactcca ccacagcatt atccagacaa ttgccttttc tggacatgct ttgtttaata
      241 ccatgttctt taaggatatt ttgatatctt ctcatacgat actgccatcc ctggtcagag
      301 tgcagaacag gatgctcgtg aggattaagc tttttgaatg cctgatcgag catattctca
      361 accatgttca tcactggtct ttccgaaagg ctgtaagaaa taacttcgtt gttgaagaga
      421 tctattactg gagacaaata cagcttgcgc ccattgactg caaattcagt aacatcggta
      481 acccacttct cgtttggccg cgtagccttg aaatctcttt ggagaacatt aggggcggtt
      541 tgccctacct ctcctctgta agagcggtat cgcttgacct taatcgctgc tttaagtgag
      601 agggttccca tcaggcgctg aacagcttta tggttaa
//
LOCUS       DAJTRW010000302          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRW010000302 DAJTRW010000000
VERSION     DAJTRW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            18..491
                     /locus_tag="OO326_004303"
     CDS             18..491
                     /locus_tag="OO326_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5968411.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTRW010000303          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRW010000303 DAJTRW010000000
VERSION     DAJTRW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..369
                     /locus_tag="OO326_004304"
     CDS             <1..369
                     /locus_tag="OO326_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968412.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
     gene            372..620
                     /locus_tag="OO326_004305"
                     /pseudo
     CDS             372..620
                     /locus_tag="OO326_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          183 a          127 c          159 g          151 t
ORIGIN      
        1 ccatccaggc ctgttgttgc agagcttggt gatgattcag gctggcattt tttcagccag
       61 agaaacccgg ataacagcat agtgttcact gttaacggac aggtaattcc gttaaattac
      121 ggaaacttcg atgcccgcta taaatatcga acagaggggg tacaggatgt acggtatggc
      181 catgaaatgt attacagccc cggcagtaac accgtttcgt ggagattttg cgcaccttcg
      241 ggacacgggc tgtcagggat ggcgatatcg gataccggcc gtaactcagc ggataacgtt
      301 gacggtgtgt attaccgacc actgcaaaaa ctgattaatg gcacctggta taacgtagcg
      361 agtatttaac aatgttgcat ttaaaaaata ttactgcggg taatccgaaa acagcagaac
      421 aatatcagat gacaaaacga tattcggtca cctggctttt ttcagaagac ggaaaaaact
      481 ggtatgaaga gctgaagaat ttcgccagga cacaattaaa ataacttaca ccggagacgg
      541 tcgcgtggtg tgggtcggta aggatgtgac aggcattgaa ccccgtaacg ccagtgttat
      601 tgaagttcct gatattaccg
//
LOCUS       DAJTRW010000304          612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRW010000304 DAJTRW010000000
VERSION     DAJTRW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>612
                     /locus_tag="OO326_004306"
     CDS             <1..>612
                     /locus_tag="OO326_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968413.1"
                     /translation="ALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPPSLTVLKA
                     SDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPDLPPALQK
                     LSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRLPELPQRL
                     QSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
BASE COUNT          167 a          187 c           93 g          165 t
ORIGIN      
        1 cagccctcaa tttaagttta ctcagattac gctctctccc atacctgccc ccgcacatac
       61 aagaacttaa catctctagc aatgagctac gctctctgcc agaactccct ccgtccttaa
      121 ctgtacttaa agccagcgat aacagactga gcaggctccc ggctcttccg cctcacctgg
      181 tcgctcttga tgtttcactt aacagagttt taacatgttt gccttctctt ccatcttcct
      241 tgcagtcact ctcagccctt ctcaatagcc tggagacgct acctgatctt cccccggctc
      301 tacaaaaact ttctgttggc aacaaccagc ttactgcctt accagaatta ccatgtgaac
      361 tacaggaact aagtgctttt gataacagat tacaagagct accgcccctt cctcaaaatc
      421 tgaggctttt aaacgttggg gaaaaccaac tacacagact gcccgaactt ccacaacgtc
      481 tgcaatcact atatatccct aacaatcagc tgaacacatt gccagacagt atcatgaatc
      541 tgcacattta tgcagatgtt aatatttata acaatccatt gtcgactcgc actctgcaag
      601 ccctgcaaag at
//
LOCUS       DAJTRW010000305          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRW010000305 DAJTRW010000000
VERSION     DAJTRW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..147)
                     /locus_tag="OO326_004307"
     CDS             complement(<1..147)
                     /locus_tag="OO326_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5968414.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLS"
     gene            complement(319..>594)
                     /locus_tag="OO326_004308"
     CDS             complement(319..>594)
                     /locus_tag="OO326_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968415.1"
                     /translation="LLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAV
                     EELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          148 a          143 c          128 g          175 t
ORIGIN      
        1 gctcaggttg tccagcaaca ggacgcgctt ctggttgtca cggatctttt tctcaacgtc
       61 ctgaatttca cgttgcagtt tgttcttacg acggaacaga cgaacaaatt ccagtacgtc
      121 ctggaatgaa ggcttggtag tttccatttt tatacccctg ataatgtgag agtcggattc
      181 gtttaagcaa ccgctattcg ttagggccaa cattactcat agctgccgca gatgacaatg
      241 cttttatccc ttttcactat catacccttt atccttgctg aatcgaagca gcagcaagat
      301 gattctgaag ttcaggaatt atttgcgcaa ggcccggcaa atcagattcg atagcaactc
      361 aagctgatgt gccgtttcca tgttcagcca cacataacca tagattggtg tttccacgac
      421 tttcaggtca tggtggttat cgagcaactg acgcagctct tccacagcat cgttcaattt
      481 ttccgtattg gcaaaaaccg gctgcggatt accttcgtac agcgcatgaa caaggctcag
      541 cagtatttgc tgcatcatgt gctgggtatc acaaagtttt tgcgcgttca ataa
//
LOCUS       DAJTRW010000306          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRW010000306 DAJTRW010000000
VERSION     DAJTRW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          218 a          105 c          121 g          150 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggggga tgaaaatacc tgaagcagta aatcatatta atgttcagaa taatattgat
      541 cttgttgatg gaaaaacgaa tccgaataaa gcaacaaaag cattacaaaa aaat
//
LOCUS       DAJTRW010000307          583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRW010000307 DAJTRW010000000
VERSION     DAJTRW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(215..>583)
                     /locus_tag="OO326_004309"
     CDS             complement(215..>583)
                     /locus_tag="OO326_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5968416.1"
                     /translation="DAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGAT
                     DKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVV
                     PIVGGIIGGLIYRTLLEKRD"
BASE COUNT          156 a          171 c          148 g          108 t
ORIGIN      
        1 aacaaaatcg ccgctgcgcg gtaccttcag cttatcccct tcggcaaccg ggtcgggcgc
       61 gaggtaacgt cctgtaagac gcgccctcag cccacatcca tgtgggctgc cccggccttc
      121 ggggaacgct tcagcgattt tgacgccacc aatacccgtg cggtttgtaa tgtcgaagat
      181 aaaacaacca gggcagataa aatgccaatg ctaattaatc acgcttttcc agcagagtcc
      241 ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag aaccacagtt
      301 gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc gggttaacag
      361 aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga ccaatagcga
      421 tcggcgcaaa acctgccgga gcgaatttgt cggttgcacc gtggatcact aacaggaagc
      481 ccgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg cctggtgaat
      541 gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtc
//
LOCUS       DAJTRW010000308          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRW010000308 DAJTRW010000000
VERSION     DAJTRW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..111
                     /locus_tag="OO326_004310"
                     /pseudo
     CDS             1..111
                     /locus_tag="OO326_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a          100 c           92 g          185 t
ORIGIN      
        1 ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct ggcccatgtt
       61 gttaccggaa agtatgcaga ccatctgcca ttataccgcc aaagtgatct actattccac
      121 acagctattt agctctcttt ttgttgagct cccatgattc tttcatttat tgttactaat
      181 tattttatgt tatcatgatg ttagaacacg ctctgctctg tgtctctatt ttatctatca
      241 tattctgagg attaactaca taacaaggcg caagatatat atacaaaaac aaataatacc
      301 gttgcaataa ttccttttag taaaggaggt gtgattaatt tcaggggatt tcagagtaaa
      361 aattgtacaa catattaaaa tatgttcatt tcgttataat acacacaagg aaatattaag
      421 ttagataata tattctaaat aaccatagat aaaaacgcac ataatgggga atattttatg
      481 aaaatacctg aagcagtaaa tcatattaat gttcagaata atattgatct tgttgatgga
      541 aaaacgaatc cgaataaagc aacaaaagca ttacaaaaaa t
//
LOCUS       DAJTRW010000309          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRW010000309 DAJTRW010000000
VERSION     DAJTRW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..528
                     /gene="rlmB"
                     /locus_tag="OO326_004311"
     CDS             <1..528
                     /gene="rlmB"
                     /locus_tag="OO326_004311"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5968417.1"
                     /translation="GIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHNLGAC
                     LRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIW
                     IVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVS
                     VATGICLFEAVRQRS"
BASE COUNT          126 a          149 c          169 g          126 t
ORIGIN      
        1 ggcattatcg cccgcgtgaa gccaggacgt cagtatcagg aaaacgatct gccggatctg
       61 atcgcttcgc tcgatcaacc gttcctgctg atcctcgacg gcgtaaccga tccgcacaac
      121 ctcggcgcgt gcctgcgtag tgcggacgcc gcaggcgttc atgcggtgat tgtgccgaaa
      181 gatcgctccg cacagctcaa cgctacggcg aaaaaagtag cctgcggcgc ggcagaaagc
      241 gttccgctga ttcgggtgac taaccttgcg cgcaccatgc gtatgttgca ggaagagaat
      301 atctggatcg tcggtacggc aggcgaggcg gatcatacac tctatcagag caaaatgacc
      361 ggacgcctgg cgctggtgat gggcgcggaa ggtgaaggta tgcgtcgcct gacccgtgaa
      421 cattgcgatg agttgatcag catcccaatg gcaggaagtg tttcttccct gaacgtttcg
      481 gttgcaaccg gaatttgctt atttgaagcg gttcgtcagc gcagctagtt cctctgaatt
      541 atgaaaagcc atccagattt ggatggtttt
//
LOCUS       DAJTRW010000310          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRW010000310 DAJTRW010000000
VERSION     DAJTRW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(14..144)
                     /locus_tag="OO326_004312"
                     /pseudo
     CDS             complement(14..144)
                     /locus_tag="OO326_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(142..237)
                     /locus_tag="OO326_004313"
                     /pseudo
     CDS             complement(142..237)
                     /locus_tag="OO326_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          160 a          113 c          147 g          150 t
ORIGIN      
        1 gtggcgcttc caatcagtag aatatgtgtt tttcaatgaa ggagccgatg acttttcgtg
       61 gatttccacg gaacgcgaga agcagattgt tttacgtgcc agccgcttga tgcgggtgcg
      121 gagggtcagg ttgttacgtt caagatatag catgtgctgg atgaagatcc cttttctggt
      181 cacctgttta tctttcgtgg ccgacggggt gacactgtta aaatgtaatg aacgcatccc
      241 acacaattaa acacggagta gtatgatggt taaaagggtg aaactggcag gtgtggagcc
      301 tcgcagcttt ccggcatcat actgcccaat acgggatgca taaagcgtcc tcagactgga
      361 gactccacaa atgaaatata caccggttgg cgttgatatg gcaaaacatg tcattcagat
      421 tcacttcatc aatgagcaca tgtcagcgcc agtgatataa gacggtaatg gggatgcgga
      481 cgatttcctg gactctcaga agatgaaaca tgtttactga agaacaaaaa attcgcgcta
      541 ttgagcttta tttcaaatat ggtaagaagc
//
LOCUS       DAJTRW010000311          569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRW010000311 DAJTRW010000000
VERSION     DAJTRW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(131..>569)
                     /locus_tag="OO326_004314"
     CDS             complement(131..>569)
                     /locus_tag="OO326_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5968418.1"
                     /translation="GKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRD
                     MINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLN
                     DAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          144 a          156 c           97 g          172 t
ORIGIN      
        1 tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg gctctttcaa
       61 atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa gaattgaagc
      121 agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata accattggtt
      181 tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca caggttctta
      241 aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg gtcatccccc
      301 gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc agcacctcac
      361 ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc cagctctttt
      421 gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc agacctgctt
      481 ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta gaggtcaaca
      541 tcaggttaaa catattcccc gtcttaccg
//
LOCUS       DAJTRW010000312          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRW010000312 DAJTRW010000000
VERSION     DAJTRW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..>541)
                     /locus_tag="OO326_004315"
     CDS             complement(<1..>541)
                     /locus_tag="OO326_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5968419.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQI"
BASE COUNT          130 a          116 c          135 g          160 t
ORIGIN      
        1 aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt cctggacgat
       61 ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa gtgccgtgag
      121 tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct gactgatcgc
      181 ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg tggctgtttt
      241 attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt tgtctgcttg
      301 ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa cttcgggcgg
      361 aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat ttttgtcagc
      421 cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata agtccgctac
      481 cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg cttgctccac
      541 a
//
LOCUS       DAJTRW010000313          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRW010000313 DAJTRW010000000
VERSION     DAJTRW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(217..>538)
                     /locus_tag="OO326_004316"
     CDS             complement(217..>538)
                     /locus_tag="OO326_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968420.1"
                     /translation="HGRLILQTDAQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIED
                     MAMFLSPLPVVLEWHCVMCEQFFHGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCG
                     GAST"
BASE COUNT          145 a          160 c          119 g          114 t
ORIGIN      
        1 gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga ttccgcacca
       61 cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat atccggtgtc
      121 gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca ggttggcaat
      181 acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac ctccgcagtc
      241 ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc tgcaataaat
      301 ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac acatcacaca
      361 atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct caatgtaagg
      421 gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac tcagggcatg
      481 taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc ggccatgg
//
LOCUS       DAJTRW010000314          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRW010000314 DAJTRW010000000
VERSION     DAJTRW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            88..>537
                     /locus_tag="OO326_004317"
     CDS             88..>537
                     /locus_tag="OO326_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968421.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLSETLWHEWVQFRQALRKPIRTELGAN"
BASE COUNT          124 a          115 c          168 g          130 t
ORIGIN      
        1 gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc caaacccttc
       61 ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag attatctggc
      121 ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc tggcctggat
      181 gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg gattgatatc
      241 gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa ttctccgcga
      301 cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt gcggcgcagt
      361 gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt tcgccagaaa
      421 aaatttgacc cgttgtcagt tgatctgccc gactggttgt cagaaacact ctggcatgag
      481 tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg cgctaac
//
LOCUS       DAJTRW010000315          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRW010000315 DAJTRW010000000
VERSION     DAJTRW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            109..390
                     /locus_tag="OO326_004318"
                     /pseudo
     CDS             109..390
                     /locus_tag="OO326_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          183 a          104 c          101 g          136 t
ORIGIN      
        1 tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca attaaaaata
       61 tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat gaaccctatg
      121 cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg aatcaaaaat
      181 ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt aattccctgt
      241 gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc tgaacttatt
      301 tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga aatctgcgcc
      361 gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact gaatttatgc
      421 cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac accaattatg
      481 aagccactgt tgcaactact gataacattg ttcacgaggt ttac
//
LOCUS       DAJTRW010000316          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRW010000316 DAJTRW010000000
VERSION     DAJTRW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..364)
                     /gene="osmC"
                     /locus_tag="OO326_004319"
     CDS             complement(<1..364)
                     /gene="osmC"
                     /locus_tag="OO326_004319"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5968422.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKA"
BASE COUNT          134 a          129 c          112 g          144 t
ORIGIN      
        1 ttgctttctg gatgatgccg tcaaaggtag aggcatcaat acccggcacc gcaacttcac
       61 tcttcagtgc gattttcgta atcgcaaaac cggcatccac tttatccagc gacacatcgg
      121 cggtggtatc aattgatgtt ggcgtgaatc ccgcttcccc cagcattaat gaaagcgcca
      181 ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc agggttggtt cctttttcgc
      241 cttcaaaacg cgtgttaaat ccatacggct gttggttcag cacgccgctc tcggtggata
      301 ctgttccctt cccgcgtttg atatcgcctt cccagtgtgc ctgacctttc ttatggattg
      361 tcattgttgc tctcctgtgg gcttaaaaag gaaattatag actacgtgag aaacagcacg
      421 aggataagca ggatattccg aataaaatac ggcgaaaata ttacttctgc acttaaatta
      481 aattcccaca atagataacc gggaatatat atctcaaga
//
LOCUS       DAJTRW010000317          509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRW010000317 DAJTRW010000000
VERSION     DAJTRW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(3..218)
                     /locus_tag="OO326_004320"
     CDS             complement(3..218)
                     /locus_tag="OO326_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5968423.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          163 a          126 c          102 g          118 t
ORIGIN      
        1 cattattcac ctctcgcagc cttacgcttg tcttctctga ttttgaagta cagatttgtc
       61 agataagtca ggaagcccag aaccagactc cccagtacac caatcgcagc ccactgtgac
      121 ggactgacct gatcaagcca ctgtaaaaac cagtagccag cactgcctgc ggaggtgccg
      181 taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc gcaaataacg
      241 gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaac cgcaaaagcg
      301 ggcgaaacga tatatacaat aaggaaagca ctctatccaa caaaccactc acagttaatc
      361 ggaataaaag cagagtgctt atgaatgatc gcctgcccga aggttagtat ttctgcacag
      421 caattttgca aaaaaaagcg atcattcata acttaaacgt ctttcagtca ctccgggatt
      481 tcccatcatc gcagactgaa agactctga
//
LOCUS       DAJTRW010000318          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRW010000318 DAJTRW010000000
VERSION     DAJTRW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>506
                     /locus_tag="OO326_004321"
     CDS             <1..>506
                     /locus_tag="OO326_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5968424.1"
                     /translation="IIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFNILR
                     EIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVAR
                     YPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYES
                     VINRIRTE"
BASE COUNT          138 a          126 c          128 g          114 t
ORIGIN      
        1 atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa aatctttaaa
       61 tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa aaccggcttc
      121 aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg tttgtatgac
      181 gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt gctgatcttt
      241 atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc ggaaatcgtt
      301 gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg gtttaacaac
      361 ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa aaccggcaac
      421 atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat ctacgaaagc
      481 gttattaacc gtattcgtac tgagtt
//
LOCUS       DAJTRW010000319          502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRW010000319 DAJTRW010000000
VERSION     DAJTRW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>502
                     /gene="yeaG"
                     /locus_tag="OO326_004322"
     CDS             <1..>502
                     /gene="yeaG"
                     /locus_tag="OO326_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5968425.1"
                     /translation="IKVLHPLLTATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFR
                     NNKNNEAFLDRVYIVKVPYCLRISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSIL
                     SRLKEPENSSIYSKMRVYDGESLKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKIL
                     SRVFNFD"
BASE COUNT          134 a          132 c          114 g          122 t
ORIGIN      
        1 gataaaagtg ctgcatccct tgttaaccgc cactcaggaa ggtaactaca acgggacgga
       61 aggtatctcc gccctgccgt tcaacgggat tattctcgcc cactcgaaca agtccgaatg
      121 ggtcactttc cgtaataaca aaaacaacga agccttcctc gaccgtgttt acatcgtgaa
      181 ggtgccgtat tgcttgcgca tttccgaaga gatcaaaatc tacgagaaat tgcttaatca
      241 cagtgaattg actcacgccc catgcgcccc tggcacgctc gaaacactgt cacgtttttc
      301 cattctttcg cgcctgaaag agccagaaaa ctccagcatt tattcaaaga tgcgggttta
      361 tgatggcgaa agtctgaaag acaccgatcc gaaagccaag tcgtatcagg aatatcgtga
      421 ctacgccggt gtcgatgaag ggatgaacgg tctgtcgacg cgttttgcgt ttaagatcct
      481 ctcccgcgtg ttcaacttcg at
//
LOCUS       DAJTRW010000320          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRW010000320 DAJTRW010000000
VERSION     DAJTRW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          145 a           90 c          120 g          146 t
ORIGIN      
        1 tccgagcaaa aagcggactt acaggatctg tgttaaggct gtcttatgat tcagacacta
       61 tcggccgtta tctgagaaat atacgagtaa ctggtaataa cacctgcaat ggtattgaca
      121 caaacattac agcagaagac tctgtcatca gacaggttta tggctgggta tttgataatg
      181 taatggtgaa tgaagttgaa accgcattat ttaatgcaag ggctctggca ctcaaaattt
      241 atagcatgtc aggctggaac ctgtagagtc ggtcttcact ttttaggcca gtgcgtaagt
      301 gttagtgtca gctcctgcca tttcagcaga ggaaattatt ctgctgatga aagctttggc
      361 atcaggattc agcctcaaac gtatgcgtgg tcgtcagagg cagttaggtc agaagcaata
      421 attttagaca gtgagaccat gtgcattggt tttaaaaatg ccgtctatgt tcatgattgc
      481 cttgatttgc atatggaaca a
//
LOCUS       DAJTRW010000321          499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRW010000321 DAJTRW010000000
VERSION     DAJTRW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(349..>499)
                     /locus_tag="OO326_004323"
     CDS             complement(349..>499)
                     /locus_tag="OO326_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968426.1"
                     /translation="LFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIF
                     PGLLN"
BASE COUNT          169 a           98 c           94 g          138 t
ORIGIN      
        1 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
       61 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
      121 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
      181 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
      241 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
      301 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
      361 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
      421 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
      481 cggtgccatg cggaacaat
//
LOCUS       DAJTRW010000322          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRW010000322 DAJTRW010000000
VERSION     DAJTRW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..>495)
                     /locus_tag="OO326_004324"
     rRNA            complement(<1..>495)
                     /locus_tag="OO326_004324"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DAJTRW010000323          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRW010000323 DAJTRW010000000
VERSION     DAJTRW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..488
                     /locus_tag="OO326_004325"
                     /pseudo
     CDS             1..488
                     /locus_tag="OO326_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          106 a          120 c          152 g          110 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatc
//
LOCUS       DAJTRW010000324          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRW010000324 DAJTRW010000000
VERSION     DAJTRW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>487
                     /locus_tag="OO326_004326"
     CDS             <1..>487
                     /locus_tag="OO326_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5968427.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAJTRW010000325          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRW010000325 DAJTRW010000000
VERSION     DAJTRW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..>478)
                     /locus_tag="OO326_004327"
     CDS             complement(<1..>478)
                     /locus_tag="OO326_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5968428.1"
                     /translation="VDAAVRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAEL
                     ESRNCGKPLHSAFNDEIPAIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVV
                     ASIAPWNYPLMMAAWKLAPALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVIN"
BASE COUNT          118 a          150 c          110 g          100 t
ORIGIN      
        1 atgttaatca cgcctgccgg gaagatatct ttcgccagct ctgccaactt caacgcggtc
       61 agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag cgccggagca
      121 agtttccacg cggccatcat cagcggataa ttccacggtg cgatagaagc cacgaccccc
      181 aacggatcgc gacggatcat cgaagtatga ccttcaagat attcacctgc cgccagacca
      241 ttcagacagc gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc cgggatttca
      301 tcattgaacg cactatgcag cggtttgcca caattacggg attccagttc ggcaaaaacc
      361 tgaccatttt cttcgataac atccgccagt ttcagcaggc attccgcacg cgctttcggt
      421 gtggtttgcc cccattcagc aaatgctgca tctgccgcac gcacagcagc atcgacct
//
LOCUS       DAJTRW010000326          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRW010000326 DAJTRW010000000
VERSION     DAJTRW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            36..419
                     /locus_tag="OO326_004328"
     CDS             36..419
                     /locus_tag="OO326_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968429.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            416..>477
                     /locus_tag="OO326_004329"
     CDS             416..>477
                     /locus_tag="OO326_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate transporter"
                     /protein_id="HCR5968430.1"
                     /translation="MNGIKTGLGITPGEHIISADS"
BASE COUNT          119 a          131 c          134 g           93 t
ORIGIN      
        1 cctgcgtgtc gtctggcggc tgaggaacat atactatgta cgcaaaatct tttctcgcac
       61 ttgatggcaa cggacgtctg accggtgccc gtaccgcaca gactgcacct tacgatagct
      121 acacctgcca cctttgcggc agtgcactca gataccaccc gcaatacgac actgaacggc
      181 cctggtttga acacgctgac gaagggctga cggagcacgg tcatgagtgc ccttatgtca
      241 ggccggaacg cagagaaata cggttgatta aacgtctgca gcaattcgtc ccggatacct
      301 tacccgtagt acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg
      361 agcagtactg cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa
      421 cggtataaaa acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactc
//
LOCUS       DAJTRW010000327          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRW010000327 DAJTRW010000000
VERSION     DAJTRW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          120 a          125 c           88 g          133 t
ORIGIN      
        1 gttttatgag gtgcaggtgc actataattc cacccgttct ggttttcaat agctacattc
       61 aacattttct ctttcctttc atcaccgaag tgaactttgt tgatgcggtg cctggtgcct
      121 ccaggtgacg ttaaccagtt aacaattaca gttggctttc ccacccaaac caataaggac
      181 taacatgact tttaactgtg ccgcgtgcgc atagccgcat tcaccgcatc acaaaattca
      241 ctttaaaaag ggcggacatc agccagcaat taaaccgatg ccgccaactg gtacttcaca
      301 cagcaatgtc gttatttaca accggaagcg cactcccacc atttaaattt cacagacaag
      361 accgactctt tatggatacc ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca
      421 cttccgggct ttggtggtat ctttactgaa gttctcacac aaccag
//
LOCUS       DAJTRW010000328          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRW010000328 DAJTRW010000000
VERSION     DAJTRW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            1..464
                     /locus_tag="OO326_004330"
                     /pseudo
     CDS             1..464
                     /locus_tag="OO326_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          168 a           80 c           90 g          126 t
ORIGIN      
        1 aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa agtaccagaa
       61 agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg cttactcaac
      121 gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt ttatggcaaa
      181 gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa ggatggcagt
      241 ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta taatattgac
      301 cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa atcctggtga
      361 cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg aatgcacggg
      421 taaaaatcat caaattaatg ttaaagccaa attgccacaa gaac
//
LOCUS       DAJTRW010000329          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRW010000329 DAJTRW010000000
VERSION     DAJTRW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>463
                     /locus_tag="OO326_004331"
     CDS             <1..>463
                     /locus_tag="OO326_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5968431.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTRW010000330          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRW010000330 DAJTRW010000000
VERSION     DAJTRW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            101..>450
                     /locus_tag="OO326_004332"
     CDS             101..>450
                     /locus_tag="OO326_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968432.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJTRW010000331          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRW010000331 DAJTRW010000000
VERSION     DAJTRW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..65)
                     /locus_tag="OO326_004333"
     CDS             complement(<1..65)
                     /locus_tag="OO326_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5968433.1"
                     /translation="MRRWISQNNIRLPRGAFFISA"
     gene            complement(40..228)
                     /locus_tag="OO326_004334"
     CDS             complement(40..228)
                     /locus_tag="OO326_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5968434.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(239..439)
                     /gene="cspG"
                     /locus_tag="OO326_004335"
     CDS             complement(239..439)
                     /gene="cspG"
                     /locus_tag="OO326_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5968435.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
BASE COUNT          128 a           97 c          105 g          110 t
ORIGIN      
        1 aaggcagata taaaaaaggc tccacgaggg agcctgatat tattttgtga aatccagcgg
       61 cgcatggtca gtgcgtattt gcagcgcgac gttatcaaag gtgatcatag tcgatttaca
      121 aaaaatacac ttagcgccca aagggtttcg ctccgtcaca tcgaatgccg atgtacggta
      181 ctgtgaacca tgacaacatg gacagcggaa atggagatgt tgagtaataa cggcaacctt
      241 agagcgtaac aacgttcgct gccgcggggc cacgttgccc ctgctcaata gaaaattcaa
      301 ctttctgatt ttcgttcagc gtgcggaatt cattgctctg gatggcggtg aaatggacga
      361 aaacgtcttt gctgccatca tcaggagtga taaagccaaa acctttatct gcgttaaacc
      421 attttactaa accagtcatt
//
LOCUS       DAJTRW010000332          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRW010000332 DAJTRW010000000
VERSION     DAJTRW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..269
                     /locus_tag="OO326_004336"
     CDS             <1..269
                     /locus_tag="OO326_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5968436.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="OO326_004337"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="OO326_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5968437.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DAJTRW010000333          425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRW010000333 DAJTRW010000000
VERSION     DAJTRW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(1..236)
                     /locus_tag="OO326_004338"
                     /pseudo
     CDS             complement(1..236)
                     /locus_tag="OO326_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT          133 a          105 c           72 g          115 t
ORIGIN      
        1 aaataaattc ccaacgggat tgccagcaac acagtaaaga ccaggctata aacaaaaatc
       61 atcgatgact aaatcacata cattgatgtc gtccactcag acagcggaat attgtactgt
      121 tctataacgc cgccgatggt cgcgcgagtc acaaccgacg ccgcaataat gaaaaccgcc
      181 aacaaaccaa agagaaattt cttccctttc gctgacttca gtttcgccat aatcatgatg
      241 taatccttgt gcagacaaat ttcttgttac ctaaacgtaa caataccatg acgtttcacc
      301 gttgtctgct ctgaaaacct cacctcttag ctggatttac aatcaacgca ttgattagtt
      361 agctaatgtt gctacttcct tccctgacta ttgatgttct gaatgaattg tcgcggtaaa
      421 atagc
//
LOCUS       DAJTRW010000334          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRW010000334 DAJTRW010000000
VERSION     DAJTRW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            203..>423
                     /locus_tag="OO326_004339"
     CDS             203..>423
                     /locus_tag="OO326_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5968438.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNA"
BASE COUNT          135 a          101 c           57 g          130 t
ORIGIN      
        1 cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta tatccgtgat
       61 gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt cagaaaacaa
      121 acttattttt attaaaatca attctttttc tttgaacatt caactgcgat atttgtaaaa
      181 acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat tccccgcata
      241 tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc aatacgcacg
      301 cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac gtactgttga
      361 gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa cgaagtgtaa
      421 tgc
//
LOCUS       DAJTRW010000335          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRW010000335 DAJTRW010000000
VERSION     DAJTRW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>398
                     /locus_tag="OO326_004340"
     CDS             <1..>398
                     /locus_tag="OO326_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR5968439.1"
                     /translation="KNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAILLS
                     FPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVYHE
                     LSRLITLRRSHNEFHPDNNFTIDTVNSSVMR"
BASE COUNT          120 a           86 c           87 g          105 t
ORIGIN      
        1 aaaaataatc ccgacggcac gcgcagtccc tatgagatga atgtcaccta tatgaatgcg
       61 ttaagccgcc gcgagagtag cgatgaagaa cgttgcacca ggtttatcct tgcccatgcg
      121 attttgttaa gtttccccgg tgtacccgcg atatatattc aaagtattct tggctcgcgt
      181 aatgattacg caggtgtcga aaaactcgga tataaccgtg cgattaaccg taaaaaatat
      241 cacagtgaag agataacacg agaactgaac gatgaagcta cattaaggca tgcggtatat
      301 catgagttgt cgcgtttaat tacacttcgt cgcagccata acgagtttca tccggataat
      361 aattttacca tcgatacggt taattcatcc gtaatgcg
//
LOCUS       DAJTRW010000336          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRW010000336 DAJTRW010000000
VERSION     DAJTRW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>394
                     /locus_tag="OO326_004341"
     CDS             <1..>394
                     /locus_tag="OO326_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5968440.1"
                     /translation="PSEINRLKALVAKLQRMQFGKSSEKLRAKTERWIQEAQERISAL
                     QEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGELSPLG
                     CDVSEQLELISSAFKVIEKQRPKLACRR"
BASE COUNT           99 a          111 c          111 g           73 t
ORIGIN      
        1 ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt atgcagttcg
       61 gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa gcacaggagc
      121 gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat gacccggtac
      181 tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca cttccccgtg
      241 ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt gaactcagtc
      301 cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt aaggttatcg
      361 aaaaacaacg ccctaaactg gcctgtcgcc ggtg
//
LOCUS       DAJTRW010000337          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRW010000337 DAJTRW010000000
VERSION     DAJTRW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          135 a           85 c           83 g           87 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg
//
LOCUS       DAJTRW010000338          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRW010000338 DAJTRW010000000
VERSION     DAJTRW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..241
                     /gene="rbsD"
                     /locus_tag="OO326_004342"
     CDS             <1..241
                     /gene="rbsD"
                     /locus_tag="OO326_004342"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5968441.1"
                     /translation="EMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIRYT
                     THEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            249..>382
                     /locus_tag="OO326_004343"
     CDS             249..>382
                     /locus_tag="OO326_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5968442.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     T"
BASE COUNT          110 a           98 c           96 g           78 t
ORIGIN      
        1 tgaaatgcag gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact
       61 ccacgaaacg ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga
      121 aattcgttac accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat
      181 tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg
      241 aggccgtcat ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa
      301 aagccctctc gggcgcagcg ttaaatgtct atccgggccg tgtgatggcg ctggtgggcg
      361 aaaacggcgc gggtaaatcc ac
//
LOCUS       DAJTRW010000339          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRW010000339 DAJTRW010000000
VERSION     DAJTRW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>375
                     /locus_tag="OO326_004344"
     CDS             <1..>375
                     /locus_tag="OO326_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5968443.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJTRW010000340          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRW010000340 DAJTRW010000000
VERSION     DAJTRW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..345
                     /locus_tag="OO326_004345"
     CDS             <1..345
                     /locus_tag="OO326_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5968444.1"
                     /translation="WARRPSGTIHHSDKGSQYVSLAYKARLKEAKLLASTGSTGDSYD
                     NAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNDDLAA"
BASE COUNT          114 a           89 c          100 g           70 t
ORIGIN      
        1 tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca gtatgtgtca
       61 ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg gagtactggt
      121 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
      181 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      241 tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc agaagcagaa
      301 aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca cagataaaac
      361 actctccagg aaa
//
LOCUS       DAJTRW010000341          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRW010000341 DAJTRW010000000
VERSION     DAJTRW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..311)
                     /locus_tag="OO326_004346"
     CDS             complement(<1..311)
                     /locus_tag="OO326_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5968445.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTRW010000342          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRW010000342 DAJTRW010000000
VERSION     DAJTRW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          100 a           87 c          113 g           71 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg
      241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa catcacgggg gccattcttg aacctgaaca actcaagaat agccgcgaaa
      361 cgttgtcatt a
//
LOCUS       DAJTRW010000343          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRW010000343 DAJTRW010000000
VERSION     DAJTRW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            91..198
                     /locus_tag="OO326_004347"
     CDS             91..198
                     /locus_tag="OO326_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5968446.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          100 a           85 c          108 g           78 t
ORIGIN      
        1 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
       61 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
      121 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
      181 ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc
      241 ccggaccagg cggcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
      301 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagatgagac ggattccgga
      361 acagaaaaac a
//
LOCUS       DAJTRW010000344          369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRW010000344 DAJTRW010000000
VERSION     DAJTRW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            175..>369
                     /locus_tag="OO326_004348"
     CDS             175..>369
                     /locus_tag="OO326_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968447.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT           93 a           84 c           86 g          106 t
ORIGIN      
        1 cagtgccgat attctcaatg tactggatag caaaaccgct gttgatacaa cgaacaccgg
       61 tgtggcgacc tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata
      121 gttcggattt tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga
      181 aacctctgtt tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc
      241 gccgccttac cgcaggatga aaagttaatt accgggcaac tggacaatga cttgcgatat
      301 atgatttatc cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcatacc
      361 ggttcattg
//
LOCUS       DAJTRW010000345          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRW010000345 DAJTRW010000000
VERSION     DAJTRW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          120 a           88 c           52 g          101 t
ORIGIN      
        1 ttctttataa atttaatctg taacaaaacc acttcccata tagaaatatt tgcaaaaata
       61 gtgcatgaaa aaagcttttc cctaccaatc cgggatataa ataatcatca gtaccacaat
      121 tattccacag caataaatct cttcccgccg ctccggacct ctgataatgg tctaaaatca
      181 ttgaggccac ttgcgacgac attgctcaat gaggaaagtt tggcgtgaat aatattcaac
      241 accagtgcag catcatcatt acatacttat gcccgccaac taccgctttc gatatattga
      301 ctcaattttt cagcctggtt aaaaatagcg accagctcag tcgcaaatat agtgactacc
      361 c
//
LOCUS       DAJTRW010000346          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRW010000346 DAJTRW010000000
VERSION     DAJTRW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            39..>357
                     /locus_tag="OO326_004349"
     CDS             39..>357
                     /locus_tag="OO326_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5968448.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAJTRW010000347          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRW010000347 DAJTRW010000000
VERSION     DAJTRW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(233..298)
                     /locus_tag="OO326_004350"
                     /pseudo
     CDS             complement(233..298)
                     /locus_tag="OO326_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT           92 a           83 c           75 g          104 t
ORIGIN      
        1 cgcataacac cctcctgtaa ctgtagttat gtacaggtgt tatttttatc tgtatggata
       61 accagtgtca attcctgata ttgtttatgg ggcatcaatg gggcatgtat gggacacttt
      121 ttatcggcga aattcgtcga agttcgtcga catggtaaac gaatcatcta tccaaccctt
      181 gaaaaacggc gctcctggac gatcttcgtc gatttttaaa aatgttgcgt cacgcgcgta
      241 acgtgacagg attaatatca caaagcaacg ccacttcacc aattgtgtaa agcgccatcg
      301 tctcaccctt gctcgcgagg tcccagttta actttagacg cagttttgcg aacc
//
LOCUS       DAJTRW010000348          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRW010000348 DAJTRW010000000
VERSION     DAJTRW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            87..194
                     /gene="ldrD"
                     /locus_tag="OO326_004351"
     CDS             87..194
                     /gene="ldrD"
                     /locus_tag="OO326_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5968449.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           84 c          100 g           75 t
ORIGIN      
        1 acctgacaac accacggggg ccattcttgc atccaaacaa tacaagaata gccgcgaaac
       61 gttgtcatta caatacaggc ggctatatga cgttcgcaga actgggcatg gccttctggc
      121 atgatttagc ggctccggtc attgctggta ttcttgccag tatgatcgtg aactggctga
      181 acaagcggaa gtaacgtgcc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg
      241 accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
      301 gcggataata ttctgcggaa tgcgcagcac agagtgaaga cgtgacggat
//
LOCUS       DAJTRW010000349          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRW010000349 DAJTRW010000000
VERSION     DAJTRW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            3..>344
                     /locus_tag="OO326_004352"
     CDS             3..>344
                     /locus_tag="OO326_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HCR5968450.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPY"
BASE COUNT           72 a           81 c           97 g           94 t
ORIGIN      
        1 caatgttcgg cataattata tctgtcatcg tattaattac gatgggctat ttgatcctga
       61 aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt cttcctgatg atgtgcggag
      121 tctggttagg gttcggtggt gtactcgatc ccgccaaaag cagcggctac ttgatcgtcg
      181 acatttataa tgaaatcctg cgcatgctgt ccaaccgcat tgccggattg gggctgtcga
      241 ttatggcggt gggcggttat gcccgctaca tggagcgcat aggagccagt tgcgcgatgg
      301 tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc gtat
//
LOCUS       DAJTRW010000350          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRW010000350 DAJTRW010000000
VERSION     DAJTRW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO326_004353"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO326_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5968451.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRW010000351          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRW010000351 DAJTRW010000000
VERSION     DAJTRW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>338
                     /locus_tag="OO326_004354"
     CDS             <1..>338
                     /locus_tag="OO326_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR5968452.1"
                     /translation="FTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLF
                     SKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMG
                     GNHPLNRFTT"
BASE COUNT          119 a           49 c           60 g          110 t
ORIGIN      
        1 attcacggaa gataaaaata aaacccttac cttcagatgg acgaaaaaac actttgagtt
       61 ttgtatggat aataaaatct ttttaaatca taaggggaag aagtcgtata aagaaaggaa
      121 tttatttctt ttctcaaaag gtgacaagat aacaattgaa gataatgtta ttgctgaaga
      181 atattcaaca atgcctgtga aaaatttctc cagtgtcggc gctttttcat ttccgacctg
      241 ccatttttca gggaatatac gtataggtcg attttgtagt attgcatcaa atgtaaaaat
      301 aatgggtgga aatcatcctt tgaatcgttt tactactc
//
LOCUS       DAJTRW010000352          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRW010000352 DAJTRW010000000
VERSION     DAJTRW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>331
                     /locus_tag="OO326_004355"
     CDS             <1..>331
                     /locus_tag="OO326_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5968453.1"
                     /translation="GKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPI
                     ALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGT
                     ETSNIQLA"
BASE COUNT           90 a           79 c           77 g           85 t
ORIGIN      
        1 tggtaaagtt attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac
       61 attgccaaat gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac
      121 tgatgtacca attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac
      181 ctttagtggt acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga
      241 tgcagcgacg aatgttgctc tacaaatgta tctgccagat ggttctacat cggtaactcc
      301 cggtaccgaa acgtcgaata ttcagttagc g
//
LOCUS       DAJTRW010000353          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRW010000353 DAJTRW010000000
VERSION     DAJTRW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..156)
                     /locus_tag="OO326_004356"
     CDS             complement(<1..156)
                     /locus_tag="OO326_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5968454.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGA"
BASE COUNT           81 a           84 c           80 g           82 t
ORIGIN      
        1 cgcgcccgac gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg
       61 aacaagttca acgatatggg cacggttcag acggcgcgat ccgcctgtta gcacactggt
      121 gccgagtttt accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg
      181 aaaaatgata tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcgag
      241 caaagcatgg cgttttgttg cgcgggatca gcaagcgtag cggcagttgt ttacgctttt
      301 attacagatt taataaatta ccacatt
//
LOCUS       DAJTRW010000354          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRW010000354 DAJTRW010000000
VERSION     DAJTRW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..>327
                     /locus_tag="OO326_004357"
     CDS             <1..>327
                     /locus_tag="OO326_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5968455.1"
                     /translation="PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTK
                     GVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPK
                     VKWRRE"
BASE COUNT          103 a           77 c           71 g           76 t
ORIGIN      
        1 taccttatct catccaggct tgggtagatc catttaataa agaggataaa agcaaagcgc
       61 cgtttaccgt tattccgcca gtatcacgat tagaaccttc gcaagaaaaa atattacgca
      121 ttattcacac caaaggtgtg tcattgcctg atgatcgcga gtcagtcttc tggctgaata
      181 ttaaaaatat tccgccatca gcgagcaaca aagcgacgaa ctcgctggaa atagcagtaa
      241 aaacgcgtat caagctgttc tggcgaccag caaatatccg ccttattccc gaagatgctg
      301 cgccgaaagt gaaatggcgc cgggaag
//
LOCUS       DAJTRW010000355          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRW010000355 DAJTRW010000000
VERSION     DAJTRW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
BASE COUNT          107 a           58 c           62 g           97 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aaca
//
LOCUS       DAJTRW010000356          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRW010000356 DAJTRW010000000
VERSION     DAJTRW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..203
                     /locus_tag="OO326_004358"
     CDS             <1..203
                     /locus_tag="OO326_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR5968456.1"
                     /translation="FTDDRLIDFMLQHPILINRPIVVTPLGTRLCRPSEVVLEILPDA
                     QKGAFSKEDGEKVVDEAGKRLK"
BASE COUNT           82 a           76 c           85 g           80 t
ORIGIN      
        1 aatttactga cgatcggtta atcgacttta tgcttcagca cccgattctg attaatcgcc
       61 cgattgtggt gacgccgctg ggaactcgcc tgtgccgccc ttcagaagtg gtgctggaaa
      121 ttctgccaga tgcgcaaaaa ggcgcattct ccaaggaaga tggcgagaaa gtggttgatg
      181 aagcgggtaa gcgcctgaaa taaagcggcg atatccactg ccgatagttg gtaaagtgcg
      241 ctttgtttat gtcggatgcg acgctaacgc gtcttatccg acctacaaaa ccgtgcaaat
      301 tcaggtaatg ctgccaactt act
//
LOCUS       DAJTRW010000357          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRW010000357 DAJTRW010000000
VERSION     DAJTRW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..281)
                     /locus_tag="OO326_004359"
     CDS             complement(<1..281)
                     /locus_tag="OO326_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5968457.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIV"
BASE COUNT          113 a           96 c           59 g           51 t
ORIGIN      
        1 acaataccgc cgacaacctg ggcaattacg tagccaacga cttcttttgc cggaaaacgt
       61 ccgccagccc ataaaccaat agtgaccgcc gggttaaaat gaccaccaga aatatgacca
      121 acagcgaagg ccatcgtcag aacggtcaaa ccaaatgcca gcgccacgcc ggcaaaacca
      181 atgcctaatt ccgggaagcc tgcggccagt acagcactac cacagccacc aaaaacaagc
      241 cagaaagtac caaaacattc agctgctaat tttctgaaca tatccacctc aattaaaaat
      301 tgaccctgtg aaaaatagg
//
LOCUS       DAJTRW010000358          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRW010000358 DAJTRW010000000
VERSION     DAJTRW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(<1..>314)
                     /locus_tag="OO326_004360"
     CDS             complement(<1..>314)
                     /locus_tag="OO326_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5968458.1"
                     /translation="ELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIH
                     SIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ
                     SL"
BASE COUNT           93 a           52 c           71 g           98 t
ORIGIN      
        1 gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt gaacattcat
       61 tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca aaataaccgt
      121 tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt gcccatatgg
      181 agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt gcgctaatct
      241 cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat acatctaatg
      301 tttccagtag ttct
//
LOCUS       DAJTRW010000359          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRW010000359 DAJTRW010000000
VERSION     DAJTRW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            108..290
                     /locus_tag="OO326_004361"
     CDS             108..290
                     /locus_tag="OO326_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5968459.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
BASE COUNT           90 a           65 c           81 g           75 t
ORIGIN      
        1 cgcttccgga tcgataccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa
       61 tctcagcgct gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg
      121 tcattggagt agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa
      181 gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcagtt aataacaatg
      241 gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgttaa tttcgattgg
      301 ttctcagatg a
//
LOCUS       DAJTRW010000360          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRW010000360 DAJTRW010000000
VERSION     DAJTRW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(52..310)
                     /locus_tag="OO326_004362"
                     /pseudo
     CDS             complement(52..310)
                     /locus_tag="OO326_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT           79 a           74 c           81 g           76 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc agatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg
//
LOCUS       DAJTRW010000361          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRW010000361 DAJTRW010000000
VERSION     DAJTRW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            46..>307
                     /locus_tag="OO326_004363"
     CDS             46..>307
                     /locus_tag="OO326_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5968460.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAA"
BASE COUNT           85 a           71 c           71 g           80 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggtttaatt cccaaaccta aaggcgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg atcgagcagc acatgtccct taatcaggct
      301 gctgctc
//
LOCUS       DAJTRW010000362          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRW010000362 DAJTRW010000000
VERSION     DAJTRW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..255
                     /locus_tag="OO326_004364"
     CDS             <1..255
                     /locus_tag="OO326_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5968461.1"
                     /translation="IGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNK
                     TIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          101 a           62 c           63 g           79 t
ORIGIN      
        1 attggacgtc tgatgaacat cagtcaggct atgattatac cacttttccc ggtttatgat
       61 gaaaaaaaac atctactgac tattgaaatt cggccaccaa tggatgcttg cattgccagc
      121 gcggacaata aaacgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggcca
      181 catccggaac agtatgtctg ggttttaaaa ttgttaaaaa cacgcaaatc aaacgaagcg
      241 gacccgtacc cttgaaatta taaagtacaa gtatttccac tagttgtttg taagcgtaca
      301 gcctg
//
LOCUS       DAJTRW010000363          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRW010000363 DAJTRW010000000
VERSION     DAJTRW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            complement(143..>303)
                     /locus_tag="OO326_004365"
     CDS             complement(143..>303)
                     /locus_tag="OO326_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5968462.1"
                     /translation="LNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYVLAHGLNTDDQL
                     AVGIVYQF"
BASE COUNT           84 a           57 c           77 g           85 t
ORIGIN      
        1 gaatgtgccg tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag atgaaatgcc
       61 aataaaaaag cctgagttcg atgagggacg aactcaggct ggtgttatgt tttacagtaa
      121 cacgcctcgg cgcgttggct gattagaact ggtagacgat accaactgcc agttggtcgt
      181 cggtgttcag tccatgagcg agaacataat cactcttatc aagcaggttg atctgataag
      241 cggtatatat gttcatattc ttattgaagt aataccaggt accgatttca atatagttca
      301 gac
//
LOCUS       DAJTRW010000364         7034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.159, whole genome shotgun sequence.
ACCESSION   DAJTRW010000364 DAJTRW010000000
VERSION     DAJTRW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.159"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            263..922
                     /locus_tag="OO326_004366"
     CDS             263..922
                     /locus_tag="OO326_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5968463.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO326_004367"
     CDS             complement(1123..1500)
                     /locus_tag="OO326_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5968464.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="OO326_004368"
     CDS             complement(1567..4533)
                     /locus_tag="OO326_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5968465.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="OO326_004369"
     CDS             complement(4536..5096)
                     /locus_tag="OO326_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5968466.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="OO326_004370"
     CDS             complement(5222..5572)
                     /locus_tag="OO326_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5968467.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="OO326_004371"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="OO326_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5968468.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT         1450 a         1968 c         2085 g         1531 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttt
//
LOCUS       DAJTRW010000365         6474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.165, whole genome shotgun sequence.
ACCESSION   DAJTRW010000365 DAJTRW010000000
VERSION     DAJTRW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.165"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..1005
                     /locus_tag="OO326_004372"
     CDS             <1..1005
                     /locus_tag="OO326_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5968469.1"
                     /translation="TIASDDLWIESLAIQQLHTTANLPNMQRVVGMPDLHPGRGYPIG
                     AAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSALDDVAEESWLEEN
                     LPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVHSGSRG
                     LGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKATGSPVLDVAHNF
                     VSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSEETLHSLAHGAGRK
                     WGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQCQGRPD
                     "
     gene            1067..1681
                     /locus_tag="OO326_004373"
                     /pseudo
     CDS             1067..1681
                     /locus_tag="OO326_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1738..3195)
                     /gene="pepD"
                     /locus_tag="OO326_004374"
     CDS             complement(1738..3195)
                     /gene="pepD"
                     /locus_tag="OO326_004374"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5968470.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3457..3915
                     /gene="gpt"
                     /locus_tag="OO326_004375"
     CDS             3457..3915
                     /gene="gpt"
                     /locus_tag="OO326_004375"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5968471.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4007..5251
                     /gene="frsA"
                     /locus_tag="OO326_004376"
     CDS             4007..5251
                     /gene="frsA"
                     /locus_tag="OO326_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5968472.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5309..5710
                     /gene="crl"
                     /locus_tag="OO326_004377"
     CDS             5309..5710
                     /gene="crl"
                     /locus_tag="OO326_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5968473.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5749..>6474)
                     /locus_tag="OO326_004378"
     CDS             complement(5749..>6474)
                     /locus_tag="OO326_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5968474.1"
                     /translation="DYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATY
                     RNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSD
                     RTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEA
                     VAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQ
                     LDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         1552 a         1608 c         1715 g         1599 t
ORIGIN      
        1 accatcgcat ctgatgacct gtggatcgag agtttagcga tccaacaatt acacaccacg
       61 gcaaatttac ccaacatgca gcgcgtagtg gggatgccag atttacaccc cggacgcggc
      121 tacccgattg gcgcagcgtt cttctccgca ggccgttttt acccagcact ggtcggcaat
      181 gatatcggct gtggcatggc gctatggcaa acagatattc tcgctcgcaa atacaacgcc
      241 gataagtttg aaaagcgatt atcagctttg gatgacgttg ctgaagaaag ctggctggag
      301 gaaaacctgc cgtcagcatt tgcacagcat ccgtggcgca gctcgcttgg ctctatcggt
      361 ggtggcaatc atttcgctga actgcaacaa gttgatcaaa tcatcaacgc tgaactgttt
      421 gcactggcag gtctggatgc gcagcatctg caactgctgg ttcatagcgg ctcgaggggg
      481 ctagggcagt ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa
      541 ggcagtgacg acgcgctggc gtttgcgcgt tttaatcgcc acctgatcgc tttgcgcata
      601 atgcaacagg ttaaggccac cggaagtccg gttctggatg tggcgcataa ctttgttagc
      661 gcgtgtcgaa tcggtgatca acaaggggtc ttacatcgta aaggtgccac gcctgatgac
      721 tgtggtctgg tggttattcc cggttcacgc ggtgattact ccaggctggt tcagcccgtc
      781 aggagtgagg aaacattgca ttcgctggcg catggggctg ggcgtaaatg ggggcgcacc
      841 gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc aactctcgcg tactgaactt
      901 ggcagtcggg taatttgtcg cgataaacaa ctcatcttcg aagaagcgcc acaagcctat
      961 aaatcggctg aaagtgtggt gcaatgtcag ggcaggccgg attaattatt cctgtcgcgc
     1021 ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctata
     1081 actctcctca gcgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag
     1141 gctaattaaa gaagctgccc gacaagacgt cgcggtaacg gtgctggaaa cagaaacagg
     1201 ccgctactct gacacgctgc gttcggcgct gatttctctg gatggcgaca acgcatgggc
     1261 gttaagcgaa agctggtgtg gcactattca gtggatttgc ccgagcccgt atcgcccaca
     1321 tcacgggcgt aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggagca
     1381 atccgatgcg atccggtatg agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa
     1441 taaaaccgac tcggcggtac gcgccacgca tttggcatcc ggtattagcg tgaaggttca
     1501 gtcagagcgc agccagcatg ctaacaaacg gctggcgcga ttgctgattg cctggaagct
     1561 ggatcagcag caataggaaa atagcgcggt gctgaaatca cagcggcgaa tgttccatca
     1621 ccagattgaa cgtggcaacc cgcgacgaac gtttacaggg atggctttta tcgaaggata
     1681 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
     1741 cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc
     1801 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
     1861 gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg
     1921 gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtaccagat gcatcaccgg
     1981 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
     2041 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgattagtga
     2101 acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
     2161 ggaggtttcg accacacctt ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc
     2221 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
     2281 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
     2341 ctcctgatag gtattcacca gagatttcag tgcgtcgact ttatcagctg cgacagcaat
     2401 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
     2461 gcgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt
     2521 acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa
     2581 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     2641 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     2701 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
     2761 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
     2821 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
     2881 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     2941 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc
     3001 catacctgcg gtagcaggtt gacgaatcag gatattacct acctgatcgc gttcgacatg
     3061 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     3121 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     3181 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     3241 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt attgagaata
     3301 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     3361 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     3421 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     3481 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     3541 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggggcgtt actggcgcgt
     3601 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     3661 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     3721 gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac
     3781 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     3841 atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     3901 atctccggtc gctaatcttt tcaacgcctg gcattgccgg gcgttgttct ttttaacttc
     3961 aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     4021 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     4081 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
     4141 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
     4201 aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     4261 atacgatacc gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     4321 gatggtgtgg caacaaaaag cctgtacgga agaagatccg caactcagtg gtcgacactg
     4381 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     4441 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     4501 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     4561 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     4621 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat
     4681 gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     4741 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     4801 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct
     4861 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     4921 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     4981 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     5041 agtgcaagga ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     5101 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     5161 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     5221 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa
     5281 aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag
     5341 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     5401 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     5461 tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta cctacagcta
     5521 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagatactga
     5581 agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct
     5641 gacgacgctg aatctgaagc tggaaccggc gaatgatttt cgtgatgaac cggtgaagtt
     5701 aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatattcatt aaaactgata
     5761 cgtcatccca accgcgacag tatcatcatt attaatattc agcttattat cgctatccag
     5821 ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat acgtagcacc
     5881 gacgtcgata taattgacca gatcttcatc accgatgccc tcaatatctt tccctttcga
     5941 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     6001 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga
     6061 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     6121 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     6181 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     6241 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     6301 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     6361 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa
     6421 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatc
//
LOCUS       DAJTRW010000366         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.175, whole genome shotgun sequence.
ACCESSION   DAJTRW010000366 DAJTRW010000000
VERSION     DAJTRW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.175"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO326_004379"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO326_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5968475.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO326_004380"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO326_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5968476.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO326_004381"
     CDS             2399..3235
                     /locus_tag="OO326_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5968477.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO326_004382"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO326_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO326_004383"
     CDS             complement(3790..4179)
                     /locus_tag="OO326_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968478.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO326_004384"
     CDS             4397..4837
                     /locus_tag="OO326_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5968479.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OO326_004385"
     CDS             complement(5582..5731)
                     /locus_tag="OO326_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5968480.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1452 a         1570 c         1552 g         1388 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgcaacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTRW010000367         2865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.221, whole genome shotgun sequence.
ACCESSION   DAJTRW010000367 DAJTRW010000000
VERSION     DAJTRW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.221"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..278
                     /locus_tag="OO326_004386"
     CDS             <1..278
                     /locus_tag="OO326_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5968481.1"
                     /translation="QQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(256..879)
                     /gene="tetR(B)"
                     /locus_tag="OO326_004387"
     CDS             complement(256..879)
                     /gene="tetR(B)"
                     /locus_tag="OO326_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5968482.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            961..2166
                     /gene="tet(B)"
                     /locus_tag="OO326_004388"
     CDS             961..2166
                     /gene="tet(B)"
                     /locus_tag="OO326_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5968483.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2279..>2865)
                     /gene="tetC"
                     /locus_tag="OO326_004389"
     CDS             complement(2279..>2865)
                     /gene="tetC"
                     /locus_tag="OO326_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5968484.1"
                     /translation="KNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKALVTK
                     GAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNK
                     NLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWF
                     MHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          754 a          537 c          591 g          983 t
ORIGIN      
        1 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
       61 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
      121 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
      181 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
      241 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
      301 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      361 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      421 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      481 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      541 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      601 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      661 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      721 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      781 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      841 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      901 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
      961 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     1021 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     1081 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     1141 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     1201 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     1261 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     1321 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     1381 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     1441 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     1501 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     1561 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     1621 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     1681 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     1741 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     1801 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     1861 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     1921 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     1981 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     2041 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     2101 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     2161 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     2221 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     2281 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     2341 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     2401 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     2461 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     2521 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     2581 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     2641 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     2701 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     2761 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     2821 gtgattgata ggtactctta aaattttctt gttgatgatt tttat
//
LOCUS       DAJTRW010000368         1763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.258, whole genome shotgun sequence.
ACCESSION   DAJTRW010000368 DAJTRW010000000
VERSION     DAJTRW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.258"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..799
                     /gene="blaOXA"
                     /locus_tag="OO326_004390"
     CDS             <1..799
                     /gene="blaOXA"
                     /locus_tag="OO326_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HCR5968485.1"
                     /translation="FLIIANIIYSSASASTDISTVASPLFEGTEGCFLLYDASTNAEI
                     AQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKT
                     WMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKIS
                     PEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNG
                     WFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            912..1703
                     /gene="aadA1"
                     /locus_tag="OO326_004391"
     CDS             912..1703
                     /gene="aadA1"
                     /locus_tag="OO326_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5968486.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          550 a          389 c          397 g          427 t
ORIGIN      
        1 ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag atatctctac
       61 tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg atgcatccac
      121 aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg caccagattc
      181 aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag atcagaaaac
      241 catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca atcatacacc
      301 aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc aaaaaattgg
      361 attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag acttctctgg
      421 agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct taaaaatttc
      481 accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc cagttaaaaa
      541 ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata atagtacaaa
      601 actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa acggatggtt
      661 tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg cacttacagg
      721 aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga tcaccattct
      781 aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc aatcaagtcg
      841 ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc ttatttcaaa
      901 cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag
      961 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1021 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1081 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1141 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1201 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1261 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1321 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1381 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1441 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1501 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1561 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     1621 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
     1681 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg
     1741 cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTRW010000369         1760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.259, whole genome shotgun sequence.
ACCESSION   DAJTRW010000369 DAJTRW010000000
VERSION     DAJTRW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.259"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..799
                     /gene="blaOXA"
                     /locus_tag="OO326_004392"
     CDS             <1..799
                     /gene="blaOXA"
                     /locus_tag="OO326_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HCR5968487.1"
                     /translation="FLIIANIIYSSASASTDISTVASPLFEGTEGCFLLYDASTNAEI
                     AQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKT
                     WMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKIS
                     PEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNG
                     WFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            912..1700
                     /gene="aadA1"
                     /locus_tag="OO326_004393"
     CDS             912..1700
                     /gene="aadA1"
                     /locus_tag="OO326_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5968488.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          547 a          389 c          397 g          427 t
ORIGIN      
        1 ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag atatctctac
       61 tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg atgcatccac
      121 aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg caccagattc
      181 aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag atcagaaaac
      241 catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca atcatacacc
      301 aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc aaaaaattgg
      361 attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag acttctctgg
      421 agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct taaaaatttc
      481 accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc cagttaaaaa
      541 ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata atagtacaaa
      601 actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa acggatggtt
      661 tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg cacttacagg
      721 aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga tcaccattct
      781 aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc aatcaagtcg
      841 ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc ttatttcaaa
      901 cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag
      961 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1021 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1081 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1141 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1201 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1261 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1321 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1381 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1441 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1501 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1561 cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc
     1621 gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca
     1681 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     1741 ggcttaactc aagcgttaga
//
LOCUS       DAJTRW010000370         1663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.263, whole genome shotgun sequence.
ACCESSION   DAJTRW010000370 DAJTRW010000000
VERSION     DAJTRW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.263"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..67
                     /locus_tag="OO326_004394"
     CDS             <1..67
                     /locus_tag="OO326_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCR5968489.1"
                     /translation="KSLWAVTVVATIVILFVALYW"
     gene            78..437
                     /gene="frdD"
                     /locus_tag="OO326_004395"
     CDS             78..437
                     /gene="frdD"
                     /locus_tag="OO326_004395"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5968490.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            500..1633
                     /locus_tag="OO326_004396"
     CDS             500..1633
                     /locus_tag="OO326_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5968491.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          397 a          425 c          433 g          408 t
ORIGIN      
        1 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
       61 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
      121 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
      181 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
      241 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
      301 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
      361 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
      421 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
      481 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
      541 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
      601 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
      661 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
      721 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
      781 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
      841 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
      901 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
      961 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     1021 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     1081 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     1141 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     1201 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     1261 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     1321 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     1381 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     1441 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     1501 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     1561 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     1621 cgctctacag taaaatttca tcgggtccga attttcggac ctt
//
LOCUS       DAJTRW010000371         1663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.264, whole genome shotgun sequence.
ACCESSION   DAJTRW010000371 DAJTRW010000000
VERSION     DAJTRW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.264"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            <1..67
                     /locus_tag="OO326_004397"
     CDS             <1..67
                     /locus_tag="OO326_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCR5968492.1"
                     /translation="KSLWAVTVVATIVILFVALYW"
     gene            78..437
                     /gene="frdD"
                     /locus_tag="OO326_004398"
     CDS             78..437
                     /gene="frdD"
                     /locus_tag="OO326_004398"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5968493.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            500..1633
                     /locus_tag="OO326_004399"
     CDS             500..1633
                     /locus_tag="OO326_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5968494.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVYRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          397 a          424 c          433 g          409 t
ORIGIN      
        1 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
       61 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
      121 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
      181 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
      241 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
      301 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
      361 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
      421 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
      481 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
      541 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgtatcgca caattacccc
      601 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
      661 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
      721 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
      781 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
      841 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
      901 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
      961 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     1021 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     1081 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     1141 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     1201 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     1261 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     1321 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     1381 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     1441 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     1501 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     1561 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     1621 cgctctacag taaaatttca tcgggtccga attttcggac ctt
//
LOCUS       DAJTRW010000372         1568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.268, whole genome shotgun sequence.
ACCESSION   DAJTRW010000372 DAJTRW010000000
VERSION     DAJTRW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.268"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            135..659
                     /gene="sat2"
                     /locus_tag="OO326_004400"
     CDS             135..659
                     /gene="sat2"
                     /locus_tag="OO326_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5968495.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            717..1508
                     /gene="aadA1"
                     /locus_tag="OO326_004401"
     CDS             717..1508
                     /gene="aadA1"
                     /locus_tag="OO326_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5968496.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          408 a          387 c          413 g          360 t
ORIGIN      
        1 taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc
       61 gcaaacctgt cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtgccag
      121 gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg
      181 ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg
      241 aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg
      301 atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac
      361 tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc
      421 gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac
      481 agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg
      541 caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag
      601 tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac
      661 aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga
      721 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      781 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      841 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      901 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      961 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1021 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1081 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1141 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1201 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1261 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1321 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1381 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1441 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1501 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     1561 gcgttaga
//
LOCUS       DAJTRW010000373         1565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.269, whole genome shotgun sequence.
ACCESSION   DAJTRW010000373 DAJTRW010000000
VERSION     DAJTRW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.269"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            135..659
                     /gene="sat2"
                     /locus_tag="OO326_004402"
     CDS             135..659
                     /gene="sat2"
                     /locus_tag="OO326_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5968497.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            717..1505
                     /gene="aadA1"
                     /locus_tag="OO326_004403"
     CDS             717..1505
                     /gene="aadA1"
                     /locus_tag="OO326_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5968498.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          405 a          387 c          413 g          360 t
ORIGIN      
        1 taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc
       61 gcaaacctgt cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtgccag
      121 gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg
      181 ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg
      241 aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg
      301 atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac
      361 tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc
      421 gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac
      481 agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg
      541 caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag
      601 tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac
      661 aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga
      721 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      781 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      841 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      901 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      961 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1021 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1081 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1141 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1201 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1261 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1321 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1381 ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg
     1441 cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca
     1501 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     1561 ttaga
//
LOCUS       DAJTRW010000374          997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.286, whole genome shotgun sequence.
ACCESSION   DAJTRW010000374 DAJTRW010000000
VERSION     DAJTRW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.286"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            4..834
                     /locus_tag="OO326_004404"
     CDS             4..834
                     /locus_tag="OO326_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5968499.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          373 a          193 c          175 g          256 t
ORIGIN      
        1 attatgaaaa acacaataca tatcaacttc gctatttttt taataattgc aaatattatc
       61 tacagcagcg ccagtgcatc aacagatatc tctactgttg catctccatt atttgaagga
      121 actgaaggtt gttttttact ttacgatgca tccacaaacg ctgaaattgc tcaattcaat
      181 aaagcaaagt gtgcaacgca aatggcacca gattcaactt tcaagatcgc attatcactt
      241 atggcatttg atgcggaaat aatagatcag aaaaccatat tcaaatggga taaaaccccc
      301 aaaggaatgg agatctggaa cagcaatcat acaccaaaga cgtggatgca attttctgtt
      361 gtttgggttt cgcaagaaat aacccaaaaa attggattaa ataaaatcaa gaattatctc
      421 aaagattttg attatggaaa tcaagacttc tctggagata aagaaagaaa caacggatta
      481 acagaagcat ggctcgaaag tagcttaaaa atttcaccag aagaacaaat tcaattcctg
      541 cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag ccatagaaaa caccatagag
      601 aacatgtatc tacaagatct ggataatagt acaaaactgt atgggaaaac tggtgcagga
      661 ttcacagcaa atagaacctt acaaaacgga tggtttgaag ggtttattat aagcaaatca
      721 ggacataaat atgtttttgt gtccgcactt acaggaaact tggggtcgaa tttaacatca
      781 agcataaaag ccaagaaaaa tgcgatcacc attctaaaca cactaaattt ataaaaaatc
      841 taatggcaaa atcgcccaac ccttcaatca agtcgggacg gccaaaagca agcttttggc
      901 tcccctcgct ggcgctcggc gccccttatt tcaaacgtta aacatcatga gggaagtggt
      961 gatcgccgaa gtatcgactc aactatcaga ggtagtt
//
LOCUS       DAJTRW010000375          996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.287, whole genome shotgun sequence.
ACCESSION   DAJTRW010000375 DAJTRW010000000
VERSION     DAJTRW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.287"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            4..833
                     /gene="blaOXA"
                     /locus_tag="OO326_004405"
                     /pseudo
     CDS             4..833
                     /gene="blaOXA"
                     /locus_tag="OO326_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
BASE COUNT          373 a          193 c          175 g          255 t
ORIGIN      
        1 attatgaaaa acacaataca tatcaacttc gctatttttt aataattgca aatattatct
       61 acagcagcgc cagtgcatca acagatatct ctactgttgc atctccatta tttgaaggaa
      121 ctgaaggttg ttttttactt tacgatgcat ccacaaacgc tgaaattgct caattcaata
      181 aagcaaagtg tgcaacgcaa atggcaccag attcaacttt caagatcgca ttatcactta
      241 tggcatttga tgcggaaata atagatcaga aaaccatatt caaatgggat aaaaccccca
      301 aaggaatgga gatctggaac agcaatcata caccaaagac gtggatgcaa ttttctgttg
      361 tttgggtttc gcaagaaata acccaaaaaa ttggattaaa taaaatcaag aattatctca
      421 aagattttga ttatggaaat caagacttct ctggagataa agaaagaaac aacggattaa
      481 cagaagcatg gctcgaaagt agcttaaaaa tttcaccaga agaacaaatt caattcctgc
      541 gtaaaattat taatcacaat ctcccagtta aaaactcagc catagaaaac accatagaga
      601 acatgtatct acaagatctg gataatagta caaaactgta tgggaaaact ggtgcaggat
      661 tcacagcaaa tagaacctta caaaacggat ggtttgaagg gtttattata agcaaatcag
      721 gacataaata tgtttttgtg tccgcactta caggaaactt ggggtcgaat ttaacatcaa
      781 gcataaaagc caagaaaaat gcgatcacca ttctaaacac actaaattta taaaaaatct
      841 aatggcaaaa tcgcccaacc cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct
      901 cccctcgctg gcgctcggcg ccccttattt caaacgttaa acatcatgag ggaagtggtg
      961 atcgccgaag tatcgactca actatcagag gtagtt
//
LOCUS       DAJTRW010000376          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.335, whole genome shotgun sequence.
ACCESSION   DAJTRW010000376 DAJTRW010000000
VERSION     DAJTRW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.335"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            20..493
                     /locus_tag="OO326_004406"
     CDS             20..493
                     /locus_tag="OO326_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5968500.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          162 a           95 c          100 g          136 t
ORIGIN      
        1 taacctctga ggaagaattg tgaaactatc actaatggta gctatatcga agaatggagt
       61 tatcgggaat ggccctgata ttccatggag tgccaaaggt gaacagctcc tgtttaaagc
      121 tattacctat aaccaatggc tgttggttgg acgcaagact tttgaatcaa tgggagcatt
      181 acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt acatctgaca atgagaacgt
      241 attgatcttt ccatcaatta aagatgcttt aaccaaccta aagaaaataa cggatcatgt
      301 cattgtttca ggtggtgggg agatatacaa aagcctgatc gatcaagtag atacactaca
      361 tatatctaca atagacatcg agccggaagg tgatgtttac tttcctgaaa tccccagcaa
      421 ttttaggcca gtttttaccc aagacttcgc ctctaacata aattatagtt accaaatctg
      481 gcaaaagggt taa
//
LOCUS       DAJTRW010000377          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600366
            SAMEA8900309-rid15818613.guided.336, whole genome shotgun sequence.
ACCESSION   DAJTRW010000377 DAJTRW010000000
VERSION     DAJTRW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900309
            Sequence Read Archive: ERR6004614
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477528.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477528.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:00:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,407
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 184 of 387
            Pseudo Genes (incomplete)         :: 106 of 387
            Pseudo Genes (internal stop)      :: 143 of 387
            Pseudo Genes (multiple problems)  :: 45 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900309-rid15818613.guided.336"
                     /serovar="S. flexneri 2a"
                     /isolate="600366"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-04-04"
     gene            20..493
                     /gene="dfrA"
                     /locus_tag="OO326_004407"
     CDS             20..493
                     /gene="dfrA"
                     /locus_tag="OO326_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCR5968501.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFAFNINYSYQIWQKG"
BASE COUNT          162 a           94 c          100 g          137 t
ORIGIN      
        1 taacctctga ggaagaattg tgaaactatc actaatggta gctatatcga agaatggagt
       61 tatcgggaat ggccctgata ttccatggag tgccaaaggt gaacagctcc tgtttaaagc
      121 tattacctat aaccaatggc tgttggttgg acgcaagact tttgaatcaa tgggagcatt
      181 acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt acatctgaca atgagaacgt
      241 attgatcttt ccatcaatta aagatgcttt aaccaaccta aagaaaataa cggatcatgt
      301 cattgtttca ggtggtgggg agatatacaa aagcctgatc gatcaagtag atacactaca
      361 tatatctaca atagacatcg agccggaagg tgatgtttac tttcctgaaa tccccagcaa
      421 ttttaggcca gtttttaccc aagacttcgc ctttaacata aattatagtt accaaatctg
      481 gcaaaagggt taa
//
